LOCUS       DADKXM010000001       106438 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.001, whole genome shotgun sequence.
ACCESSION   DADKXM010000001 DADKXM010000000
VERSION     DADKXM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 106438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.001"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..320
                     /locus_tag="JQR05_000001"
     CDS             <1..320
                     /locus_tag="JQR05_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5322269.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JQR05_000002"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JQR05_000002"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY5322270.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JQR05_000003"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JQR05_000003"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY5322271.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVLPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /locus_tag="JQR05_000004"
     CDS             complement(2164..3066)
                     /locus_tag="JQR05_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY5322272.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JQR05_000005"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JQR05_000005"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY5322273.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JQR05_000006"
     CDS             complement(3724..4953)
                     /locus_tag="JQR05_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY5322274.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="JQR05_000007"
     CDS             complement(5017..5457)
                     /locus_tag="JQR05_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY5322275.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JQR05_000008"
     CDS             complement(5562..6332)
                     /locus_tag="JQR05_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5322276.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JQR05_000009"
     CDS             complement(6329..7255)
                     /locus_tag="JQR05_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5322277.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JQR05_000010"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JQR05_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY5322278.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JQR05_000011"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JQR05_000011"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY5322279.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JQR05_000012"
     CDS             8809..11199
                     /locus_tag="JQR05_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY5322280.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12796)
                     /gene="cueO"
                     /locus_tag="JQR05_000013"
     CDS             complement(11246..12796)
                     /gene="cueO"
                     /locus_tag="JQR05_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY5322281.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVRLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12962..13309
                     /locus_tag="JQR05_000014"
     CDS             12962..13309
                     /locus_tag="JQR05_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY5322282.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13415..14281
                     /gene="speE"
                     /locus_tag="JQR05_000015"
     CDS             13415..14281
                     /gene="speE"
                     /locus_tag="JQR05_000015"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY5322283.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVNNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14297..15091
                     /gene="speD"
                     /locus_tag="JQR05_000016"
     CDS             14297..15091
                     /gene="speD"
                     /locus_tag="JQR05_000016"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY5322284.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15129..15491)
                     /locus_tag="JQR05_000017"
     CDS             complement(15129..15491)
                     /locus_tag="JQR05_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY5322285.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JQR05_000018"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JQR05_000018"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY5322286.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JQR05_000019"
     CDS             18618..20471
                     /locus_tag="JQR05_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY5322287.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWLQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAEEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQASAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(20542..21966)
                     /gene="lpdA"
                     /locus_tag="JQR05_000020"
     CDS             complement(20542..21966)
                     /gene="lpdA"
                     /locus_tag="JQR05_000020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY5322288.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22291..24183)
                     /gene="aceF"
                     /locus_tag="JQR05_000021"
     CDS             complement(22291..24183)
                     /gene="aceF"
                     /locus_tag="JQR05_000021"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY5322289.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAATAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24198..26861)
                     /gene="aceE"
                     /locus_tag="JQR05_000022"
     CDS             complement(24198..26861)
                     /gene="aceE"
                     /locus_tag="JQR05_000022"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY5322290.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27022..27786)
                     /gene="pdhR"
                     /locus_tag="JQR05_000023"
     CDS             complement(27022..27786)
                     /gene="pdhR"
                     /locus_tag="JQR05_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY5322291.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28327..29700
                     /gene="aroP"
                     /locus_tag="JQR05_000024"
     CDS             28327..29700
                     /gene="aroP"
                     /locus_tag="JQR05_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY5322292.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29743..30597)
                     /gene="ampE"
                     /locus_tag="JQR05_000025"
     CDS             complement(29743..30597)
                     /gene="ampE"
                     /locus_tag="JQR05_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY5322293.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30594..31145)
                     /gene="ampD"
                     /locus_tag="JQR05_000026"
     CDS             complement(30594..31145)
                     /gene="ampD"
                     /locus_tag="JQR05_000026"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY5322294.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31233..32126
                     /gene="nadC"
                     /locus_tag="JQR05_000027"
     CDS             31233..32126
                     /gene="nadC"
                     /locus_tag="JQR05_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY5322295.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLTENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32329..32769
                     /gene="ppdD"
                     /locus_tag="JQR05_000028"
     CDS             32329..32769
                     /gene="ppdD"
                     /locus_tag="JQR05_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY5322296.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32779..34164
                     /gene="gspE"
                     /locus_tag="JQR05_000029"
     CDS             32779..34164
                     /gene="gspE"
                     /locus_tag="JQR05_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY5322297.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34154..35356
                     /gene="hofC"
                     /locus_tag="JQR05_000030"
     CDS             34154..35356
                     /gene="hofC"
                     /locus_tag="JQR05_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY5322298.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTSELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35391..36434)
                     /gene="guaC"
                     /locus_tag="JQR05_000031"
     CDS             complement(35391..36434)
                     /gene="guaC"
                     /locus_tag="JQR05_000031"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY5322299.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36658..37278
                     /gene="coaE"
                     /locus_tag="JQR05_000032"
     CDS             36658..37278
                     /gene="coaE"
                     /locus_tag="JQR05_000032"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY5322300.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37278..38021
                     /gene="zapD"
                     /locus_tag="JQR05_000033"
     CDS             37278..38021
                     /gene="zapD"
                     /locus_tag="JQR05_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY5322301.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38031..38228
                     /gene="yacG"
                     /locus_tag="JQR05_000034"
     CDS             38031..38228
                     /gene="yacG"
                     /locus_tag="JQR05_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY5322302.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38328..38717)
                     /gene="mutT"
                     /locus_tag="JQR05_000035"
     CDS             complement(38328..38717)
                     /gene="mutT"
                     /locus_tag="JQR05_000035"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY5322303.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38777..41482)
                     /gene="secA"
                     /locus_tag="JQR05_000036"
     CDS             complement(38777..41482)
                     /gene="secA"
                     /locus_tag="JQR05_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY5322304.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41544..42056)
                     /gene="secM"
                     /locus_tag="JQR05_000037"
     CDS             complement(41544..42056)
                     /gene="secM"
                     /locus_tag="JQR05_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY5322305.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42286..43203)
                     /gene="lpxC"
                     /locus_tag="JQR05_000038"
     CDS             complement(42286..43203)
                     /gene="lpxC"
                     /locus_tag="JQR05_000038"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY5322306.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43304..44455)
                     /gene="ftsZ"
                     /locus_tag="JQR05_000039"
     CDS             complement(43304..44455)
                     /gene="ftsZ"
                     /locus_tag="JQR05_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY5322307.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44516..45778)
                     /gene="ftsA"
                     /locus_tag="JQR05_000040"
     CDS             complement(44516..45778)
                     /gene="ftsA"
                     /locus_tag="JQR05_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY5322308.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45775..46605)
                     /gene="ftsQ"
                     /locus_tag="JQR05_000041"
     CDS             complement(45775..46605)
                     /gene="ftsQ"
                     /locus_tag="JQR05_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY5322309.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46607..47527)
                     /locus_tag="JQR05_000042"
     CDS             complement(46607..47527)
                     /locus_tag="JQR05_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY5322310.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47520..48995)
                     /gene="murC"
                     /locus_tag="JQR05_000043"
     CDS             complement(47520..48995)
                     /gene="murC"
                     /locus_tag="JQR05_000043"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY5322311.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49049..50116)
                     /gene="murG"
                     /locus_tag="JQR05_000044"
     CDS             complement(49049..50116)
                     /gene="murG"
                     /locus_tag="JQR05_000044"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY5322312.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50113..51357)
                     /gene="ftsW"
                     /locus_tag="JQR05_000045"
     CDS             complement(50113..51357)
                     /gene="ftsW"
                     /locus_tag="JQR05_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY5322313.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51357..52673)
                     /gene="murD"
                     /locus_tag="JQR05_000046"
     CDS             complement(51357..52673)
                     /gene="murD"
                     /locus_tag="JQR05_000046"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY5322314.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFACLAKELG"
     gene            complement(52676..53758)
                     /gene="mraY"
                     /locus_tag="JQR05_000047"
     CDS             complement(52676..53758)
                     /gene="mraY"
                     /locus_tag="JQR05_000047"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY5322315.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53752..55110)
                     /gene="murF"
                     /locus_tag="JQR05_000048"
     CDS             complement(53752..55110)
                     /gene="murF"
                     /locus_tag="JQR05_000048"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY5322316.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55107..56594)
                     /gene="murE"
                     /locus_tag="JQR05_000049"
     CDS             complement(55107..56594)
                     /gene="murE"
                     /locus_tag="JQR05_000049"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY5322317.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56581..58347)
                     /locus_tag="JQR05_000050"
     CDS             complement(56581..58347)
                     /locus_tag="JQR05_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY5322318.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGTIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58363..58728)
                     /gene="ftsL"
                     /locus_tag="JQR05_000051"
     CDS             complement(58363..58728)
                     /gene="ftsL"
                     /locus_tag="JQR05_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY5322319.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58725..59666)
                     /gene="rsmH"
                     /locus_tag="JQR05_000052"
     CDS             complement(58725..59666)
                     /gene="rsmH"
                     /locus_tag="JQR05_000052"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY5322320.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59668..60126)
                     /gene="mraZ"
                     /locus_tag="JQR05_000053"
     CDS             complement(59668..60126)
                     /gene="mraZ"
                     /locus_tag="JQR05_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY5322321.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(60728..61732)
                     /gene="cra"
                     /locus_tag="JQR05_000054"
     CDS             complement(60728..61732)
                     /gene="cra"
                     /locus_tag="JQR05_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY5322322.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(61814..61900)
                     /locus_tag="JQR05_000055"
     CDS             complement(61814..61900)
                     /locus_tag="JQR05_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY5322323.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(61912..62403)
                     /gene="ilvN"
                     /locus_tag="JQR05_000056"
     CDS             complement(61912..62403)
                     /gene="ilvN"
                     /locus_tag="JQR05_000056"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY5322324.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGANVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(62406..64130)
                     /gene="ilvI"
                     /locus_tag="JQR05_000057"
     CDS             complement(62406..64130)
                     /gene="ilvI"
                     /locus_tag="JQR05_000057"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY5322325.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(64448..65392)
                     /gene="leuO"
                     /locus_tag="JQR05_000058"
     CDS             complement(64448..65392)
                     /gene="leuO"
                     /locus_tag="JQR05_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY5322326.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPS
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            66052..66138
                     /gene="leuL"
                     /locus_tag="JQR05_000059"
     CDS             66052..66138
                     /gene="leuL"
                     /locus_tag="JQR05_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY5322327.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            66231..67802
                     /gene="leuA"
                     /locus_tag="JQR05_000060"
     CDS             66231..67802
                     /gene="leuA"
                     /locus_tag="JQR05_000060"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY5322328.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            67802..68893
                     /gene="leuB"
                     /locus_tag="JQR05_000061"
     CDS             67802..68893
                     /gene="leuB"
                     /locus_tag="JQR05_000061"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY5322329.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            68896..70296
                     /gene="leuC"
                     /locus_tag="JQR05_000062"
     CDS             68896..70296
                     /gene="leuC"
                     /locus_tag="JQR05_000062"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY5322330.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKIMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            70307..70912
                     /gene="leuD"
                     /locus_tag="JQR05_000063"
     CDS             70307..70912
                     /gene="leuD"
                     /locus_tag="JQR05_000063"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY5322331.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(70961..72139)
                     /gene="setA"
                     /locus_tag="JQR05_000064"
     CDS             complement(70961..72139)
                     /gene="setA"
                     /locus_tag="JQR05_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY5322332.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(72241..72372)
                     /gene="sgrT"
                     /locus_tag="JQR05_000065"
     CDS             complement(72241..72372)
                     /gene="sgrT"
                     /locus_tag="JQR05_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY5322333.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            72461..74116
                     /locus_tag="JQR05_000066"
     CDS             72461..74116
                     /locus_tag="JQR05_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY5322334.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEMPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      74150..74249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            74280..75263
                     /gene="thiB"
                     /locus_tag="JQR05_000067"
     CDS             74280..75263
                     /gene="thiB"
                     /locus_tag="JQR05_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY5322335.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESNLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            75239..76849
                     /gene="thiP"
                     /locus_tag="JQR05_000068"
     CDS             75239..76849
                     /gene="thiP"
                     /locus_tag="JQR05_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY5322336.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMVSRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            76833..77531
                     /gene="thiQ"
                     /locus_tag="JQR05_000069"
     CDS             76833..77531
                     /gene="thiQ"
                     /locus_tag="JQR05_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY5322337.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(77645..78409)
                     /locus_tag="JQR05_000070"
     CDS             complement(77645..78409)
                     /locus_tag="JQR05_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5322338.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(78495..79373)
                     /gene="araC"
                     /locus_tag="JQR05_000071"
     CDS             complement(78495..79373)
                     /gene="araC"
                     /locus_tag="JQR05_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY5322339.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            79712..81412
                     /gene="araB"
                     /locus_tag="JQR05_000072"
     CDS             79712..81412
                     /gene="araB"
                     /locus_tag="JQR05_000072"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY5322340.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            81423..82925
                     /gene="araA"
                     /locus_tag="JQR05_000073"
     CDS             81423..82925
                     /gene="araA"
                     /locus_tag="JQR05_000073"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY5322341.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGDFHAFT
                     TTFKDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVFGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            83125..83820
                     /gene="araD"
                     /locus_tag="JQR05_000074"
     CDS             83125..83820
                     /gene="araD"
                     /locus_tag="JQR05_000074"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY5322342.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            83895..86246
                     /gene="polB"
                     /locus_tag="JQR05_000075"
     CDS             83895..86246
                     /gene="polB"
                     /locus_tag="JQR05_000075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY5322343.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNNELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQKG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            86411..89317
                     /gene="rapA"
                     /locus_tag="JQR05_000076"
     CDS             86411..89317
                     /gene="rapA"
                     /locus_tag="JQR05_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY5322344.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            89329..89988
                     /gene="rluA"
                     /locus_tag="JQR05_000077"
     CDS             89329..89988
                     /gene="rluA"
                     /locus_tag="JQR05_000077"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY5322345.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(90105..90920)
                     /gene="djlA"
                     /locus_tag="JQR05_000078"
     CDS             complement(90105..90920)
                     /gene="djlA"
                     /locus_tag="JQR05_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY5322346.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            91175..93529
                     /gene="lptD"
                     /locus_tag="JQR05_000079"
     CDS             91175..93529
                     /gene="lptD"
                     /locus_tag="JQR05_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY5322347.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEALGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            93582..94868
                     /gene="surA"
                     /locus_tag="JQR05_000080"
     CDS             93582..94868
                     /gene="surA"
                     /locus_tag="JQR05_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY5322348.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDTTLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRGRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            94868..95857
                     /gene="pdxA"
                     /locus_tag="JQR05_000081"
     CDS             94868..95857
                     /gene="pdxA"
                     /locus_tag="JQR05_000081"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY5322349.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            95854..96675
                     /gene="rsmA"
                     /locus_tag="JQR05_000082"
     CDS             95854..96675
                     /gene="rsmA"
                     /locus_tag="JQR05_000082"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY5322350.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDHFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            96678..97055
                     /gene="apaG"
                     /locus_tag="JQR05_000083"
     CDS             96678..97055
                     /gene="apaG"
                     /locus_tag="JQR05_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY5322351.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVHGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            97062..97904
                     /gene="apaH"
                     /locus_tag="JQR05_000084"
     CDS             97062..97904
                     /gene="apaH"
                     /locus_tag="JQR05_000084"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY5322352.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(97982..98461)
                     /gene="folA"
                     /locus_tag="JQR05_000085"
     CDS             complement(97982..98461)
                     /gene="folA"
                     /locus_tag="JQR05_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY5322353.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(98652..100513)
                     /gene="kefC"
                     /locus_tag="JQR05_000086"
                     /pseudo
     CDS             complement(98652..100513)
                     /gene="kefC"
                     /locus_tag="JQR05_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(100506..101036)
                     /gene="kefF"
                     /locus_tag="JQR05_000087"
     CDS             complement(100506..101036)
                     /gene="kefF"
                     /locus_tag="JQR05_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY5322354.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(101084..102478)
                     /locus_tag="JQR05_000088"
     CDS             complement(101084..102478)
                     /locus_tag="JQR05_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5322355.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(102537..102824)
                     /gene="fixX"
                     /locus_tag="JQR05_000089"
     CDS             complement(102537..102824)
                     /gene="fixX"
                     /locus_tag="JQR05_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY5322356.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(102821..104107)
                     /locus_tag="JQR05_000090"
     CDS             complement(102821..104107)
                     /locus_tag="JQR05_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5322357.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEASAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTSENTLSLGLVCGLHHLHDAKKSVPQMLEDFKHHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(104158..105022)
                     /locus_tag="JQR05_000091"
                     /pseudo
     CDS             complement(104158..105022)
                     /locus_tag="JQR05_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixB"
     gene            complement(105037..105807)
                     /locus_tag="JQR05_000092"
     CDS             complement(105037..105807)
                     /locus_tag="JQR05_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY5322358.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            106279..>106438
                     /locus_tag="JQR05_000093"
     CDS             106279..>106438
                     /locus_tag="JQR05_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="catalyzes the exchange of L-carnitine for
                     gamma-butyrobetaine in carnitine metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY5322359.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGW"
BASE COUNT        25750 a        29312 c        26698 g        24678 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac gatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcgaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcaagctgt tcaacctggt cctgccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcgttc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgctatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacgctcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgcggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggccttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggtgag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc ccggtcttgc
    12361 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacctcaccg
    12421 agcgcttgcc acctggcgga ataattccct gtgggccgcc gtcgacgtca cccggtactt
    12481 ccagcccgtg ccagtgtaac gtcgtctctt ccgccagttg gttgtagata tcaaccgtta
    12541 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12601 tagttgcagt tttctcgcca aaggtggact gacctgcgcc aatagttaac tgaatgcgat
    12661 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    12721 ctgcacggct ccacagtggc aaggctgaag ccacacccag tgcgacggaa tatttcaaga
    12781 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    12841 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    12901 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    12961 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt gcgcaaacag
    13021 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13081 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13141 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13201 tctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaaatg
    13261 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13321 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13381 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13441 aacgctacac gaccagtttg ggcagtactt tgcggtaaat aacgttctgt atcatgaaaa
    13501 gaccgatcac caggatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13561 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13621 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    13681 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    13741 cgatgcgggt gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    13801 cgacgatccg cgctttcaac tagtgatcga cgatggcgtc aatttcgtta atcaaaccag
    13861 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagcct
    13921 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    13981 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc atcgcaaact
    14041 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14101 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14161 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14221 tacagcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14281 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14341 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14401 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14461 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14521 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14581 agaacacccc ggcccactgc cagaaacggt ggttgctcat ctcgataaaa gtcatatttg
    14641 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    14701 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    14761 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttactc gcgacattaa
    14821 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    14881 tatgaaggcg ctgtatgaca tggtggatgt aaacgtctat caggaaaata tcttccatac
    14941 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15001 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15061 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15121 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15181 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc ttccatttca
    15241 tcgccagcga attccagttg attggcgcga accatcacct cttcagcgtc catccacagg
    15301 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15361 tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa ccagtgcccg
    15421 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15481 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt
    15541 gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15601 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatggttgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccgaag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcatcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca acaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcgg
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcggca ggtcggtaat
    20581 gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg
    20641 gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc cgatttcacc
    20701 cagcagctcg ccgccgttgg taccaacaat cgcaccaccg atcacacggt gagattcttt
    20761 gtcgaaaatc agcttggtca taccgtctgc gcagtcagaa gcgatagcac gaccagaagc
    20821 agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt ctttctcagt
    20881 cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc
    20941 gaaatagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt
    21001 gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt
    21061 acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtca acttccacgc cagctttgcc
    21121 tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg
    21181 ctgcggttca gctggtgctt ttttgccttc catcgtcaca taaataccgt cttctttcgc
    21241 ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt
    21301 gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca tttcaaccac
    21361 gtcaatctgt gaacccagcg cgtggtaaac ggtgcccatt tccagaccga tgataccgcc
    21421 acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca
    21481 gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac ccgctgcaat
    21541 gatcgcattg tcgaagttga tcacggtttt gccgttctca ccttcaactt ccagggtgtt
    21601 agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc
    21661 agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcaat
    21721 atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat
    21781 aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca ggcaaacacc
    21841 gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa
    21901 ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt
    21961 actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct ttcaggccgc
    22021 agcggcgtta gcttcacgcc ctcaccccag ccacttactt atgtaagctc ctggggattc
    22081 tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta ataccctaac
    22141 caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac
    22201 aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag attaccagaa
    22261 aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa tgtcagacag
    22321 cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca cgcggtggtc
    22381 gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat tccacaccgg
    22441 ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca caatcggcgc
    22501 gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc cctgcatttc
    22561 gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct cgcgagacag
    22621 ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac cgttcggggt
    22681 atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac cgtcttccga
    22741 cagcgaacta ttgaagcgag gcatctgctc aagagctgca gcaacggctt tcatgatgaa
    22801 gacaaccggg gtgatcttca catccagctt acgtttcgcc gcttcttcgt tctgctgttt
    22861 acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgggtaacat gcgggatcat
    22921 cacccagtta cggctcaggt tagcaccaga aattttctgg atacggccca gttccacttc
    22981 ttcgatttcg ccaaacttgc tgaagtccac cttcggccac ggcagcatgc cagggatacc
    23041 accgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca cgtaagcctg
    23101 aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg ccaggttaac
    23161 gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc
    23221 agcaaattca gatttgcctt ccgctttcgc agccggagct gctgccggag cttcagcttt
    23281 tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc
    23341 ttcaacttcg aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt
    23401 cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc
    23461 tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt
    23521 cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg
    23581 tgcaggggtc gctgccggag ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc
    23641 acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac
    23701 tttgatctct ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc
    23761 ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc
    23821 ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc
    23881 cgcgggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag ttgcagcgtc
    23941 tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc
    24001 aacagagact ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc
    24061 tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac
    24121 caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc
    24181 cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat
    24241 ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc
    24301 agcgccgcaa ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg
    24361 ctgtcggaac gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa
    24421 gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg
    24481 ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag
    24541 cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca
    24601 gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg
    24661 gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag
    24721 atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg
    24781 ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga
    24841 ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc
    24901 gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc
    24961 agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg gtcgccagcc
    25021 gcccagcaca gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg
    25081 atcatcggca gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg
    25141 cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca
    25201 acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga
    25261 cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga
    25321 tctttgatcg acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta
    25381 gagatctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca
    25441 agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga
    25501 gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg
    25561 atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg
    25621 ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa
    25681 cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg
    25741 aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg
    25801 tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga
    25861 acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc
    25921 agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac
    25981 atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc
    26041 ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc
    26101 aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc
    26161 atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg
    26221 tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggacc cagccccata
    26281 gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg
    26341 ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc
    26401 aggaaagcac gggcgtaaac gcccggggag atgtggccct ggaagtaaac caagtcgccg
    26461 ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg
    26521 gaagactgga aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc
    26581 accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta
    26641 cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg
    26701 cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac
    26761 tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga
    26821 gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc
    26881 taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag
    26941 acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac atcttctgga
    27001 taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc
    27061 acggcggctc tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg
    27121 atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt
    27181 gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg
    27241 gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt
    27301 gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag
    27361 atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc
    27421 ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaagg cgtgtcgtgt
    27481 ttcgagcaag tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct
    27541 gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa
    27601 gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg
    27661 ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat
    27721 caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta
    27781 ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt
    27841 ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca
    27901 tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca
    27961 caaatgtaga ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg
    28021 aattgaaccg attcacttac caattttttg atttttaagt caataaaaac gaatttaaat
    28081 tcactctgca tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa
    28141 ctcatcttca accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc
    28201 tgacggaagt ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa
    28261 acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga
    28321 ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc
    28381 catattcagc ttatcgcgct gggtggcgcg atagggacag ggctattcct gggtagcgcc
    28441 tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc
    28501 gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc
    28561 tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac
    28621 tactgggtac tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt
    28681 cagttctggt atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt
    28741 aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt
    28801 atcaaagtta ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc
    28861 aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac
    28921 ggcttcaccg gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa
    28981 ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact
    29041 aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca
    29101 ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag
    29161 ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc
    29221 gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg
    29281 aacgcgccaa aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg
    29341 gtgtctgcgc tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca
    29401 tttggcctgt taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt
    29461 ctggcgcata tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct
    29521 gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg
    29581 attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc
    29641 gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa
    29701 tctctctacg ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta
    29761 aatcgtcagt agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac
    29821 cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc
    29881 cagacgcgtt aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc
    29941 cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg
    30001 cacccaatcc agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt
    30061 ctggtatcgc gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac
    30121 gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt
    30181 aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg
    30241 gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg
    30301 tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag
    30361 caaaccaatc agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc
    30421 gcgtaacagt aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag
    30481 agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc
    30541 gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt
    30601 gtctccttgc tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt
    30661 ttccgatccg gcgcaatatc acaatggccg gtcatgttat tagcgatatc gggatagcga
    30721 tctatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga
    30781 gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga
    30841 tactgagaga ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca
    30901 ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa
    30961 ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac
    31021 tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg
    31081 cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac
    31141 aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac
    31201 cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca
    31261 cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg
    31321 cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt
    31381 taacggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg
    31441 gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct
    31501 ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc
    31561 catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag
    31621 gagttgccag taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt
    31681 tggatacgcg caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg
    31741 gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata
    31801 ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg
    31861 cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag
    31921 ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca
    31981 ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat
    32041 ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac
    32101 tcgacctttc aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg
    32161 ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc
    32221 aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc
    32281 tgatggcgtt tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa
    32341 cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat tttaagcgcc
    32401 attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa
    32461 acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat
    32521 acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc
    32581 atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc
    32641 gtcgtcatga cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc
    32701 aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac
    32761 gccaactaag gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag
    32821 ggagtcttgc tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca
    32881 catgagttgc tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg
    32941 acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc cgtagctgtt
    33001 caggagaagc atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa
    33061 caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt
    33121 atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca
    33181 gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg
    33241 caattcactg tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt
    33301 cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt
    33361 aacacgctgg gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca
    33421 cagggactgg tgctggtaac tggccctaca ggcagcggca aaacggtcac gctttatagt
    33481 gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga tccggttgag
    33541 atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccgggct cacctttcag
    33601 ggcgttttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg agagatccgc
    33661 gatggcgaaa cggcagaaat tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtcc
    33721 acgctacaca ccaattccac cactgaaacg ctggtacgtt tacagcaaat gggggtcgcc
    33781 cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt acgtaaactt
    33841 tgcccacatt gtcgccgaca gcaaggggag cccatccata ttccagacac tgtatggccg
    33901 tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg tttttatggt
    33961 cgtaccgcct tgtttgaagt cctgcccatt acaccagcca tacgccagct tatttccgct
    34021 aatactgaag ttgaatcgct ggaaacgcac gcccgacagg cgggtatgcg tacgcttttt
    34081 gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta
    34141 ctggggatgc cgcatggcaa gtaagcaact ctggcgctgg catggcataa ccggcgacgg
    34201 caatgcgcaa gatgggatgc tatgggcaga gagccgtact ttactgctta tggcactaca
    34261 gcaacagatg gttaccccac taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg
    34321 aggagataaa agcgcggaag tcattcatca actggcgacg ctacttaaag ccgggttaac
    34381 gctttctgaa gggctggctc tgctggcgga acagcatccc agtaagcaat ggcaagcgtt
    34441 gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg ccttattacc
    34501 ctggtcagag gcatttccgc cgctctatca ggcgatgatc cgcacgagtg aactgaccgg
    34561 taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaatcccagc gtcagttgac
    34621 cgacaaagtg aaatcagcgt tgcattatcc catcatcatt ttagcgatgg caatcatggt
    34681 ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata agaccttcaa
    34741 caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta gtggcgaatg
    34801 gggctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt tgctgatgcg
    34861 ccgcccgacc tggcttatcg cgcggcagaa attgctgtta cgcatcccga ttatgggttc
    34921 actgatgagg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga cacaaagtgc
    34981 aggcattact tttttacagg gcgtagagag cgtcagagaa acaatgcgct gcccgtactg
    35041 ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca tctggctggc
    35101 gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc
    35161 atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg ataacacaat
    35221 ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca taacgggagg
    35281 aattatcggt acgctggtgg tggcaatgta tctgccaatt ttccatttag gcgatgcgat
    35341 gagtgggatg ggataacgct ggcgtgttgc tccacgccag cgttgggaga ttacaggttg
    35401 ttgaagatgc ggttttcttg ttccagcaca cgaataaacg tggtgcgctt ggtcagctct
    35461 ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc
    35521 gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc tgctgcgcgg
    35581 tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat gccgtagaac
    35641 agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc
    35701 agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggctttcgc cacatcgccc
    35761 ggcgtggtac agccaccgtc gctgacgatc attccgccca gaccgtgcgc cgcatcggca
    35821 cattcgatta ccgcagaaag ctgcggataa ccgacgcctg ttttgacgcg agttgtacaa
    35881 acagaacctg ggccaatgcc aactttaacg atatcagcac cggagaggat aagctcctca
    35941 cacatttcac cagtcactac gttgccagca caaatggttt tggtcggcca cgcttcacgc
    36001 gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacatc aatacaaacg
    36061 aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac
    36121 gtgccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt gataaacgct
    36181 tgccactctt cgacagaaaa gtgtttatgc acagcagtca gaatatcaaa agaagccagc
    36241 gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcgataat cggcacgccg
    36301 gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac atcggaacgg
    36361 cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa cttcagatct
    36421 tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag agcgcaactc
    36481 cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt gtctttttta
    36541 cgctacaatc ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcactt
    36601 tcgcgcgctc atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg
    36661 gggtatatag ttgccttaac gggaggcatt ggcagtggta agagtaccgt tgccaatgcg
    36721 tttgctgatc tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa
    36781 ccaggtgcac ctgcgctaca tgccattgct gatcactttg gcgaaaacat gattgctgct
    36841 gatggaacat tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa
    36901 aactggctta acgacctgct gcatccgctg attcagcaag agacgcaaca ccagatccag
    36961 caagcaactt ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat
    37021 aaaaaagcga atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc
    37081 atgcagcgcg atgatgtaac tcgcgaacat gtcgaacaaa tccttgctgc tcaggcaacg
    37141 cgcgaagccc gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct
    37201 atcgcatcgg atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc
    37261 tcacaggaaa aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa
    37321 tgcgtacatg gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg
    37381 ttgaccacgc tggcgcgctg catttcttcc gtaatgtcag tgaattactg gatgttttcg
    37441 agcgcggcga agtccgcact gagctgttga aagaacttaa ccggcagcaa cgtaaactcc
    37501 agacctggat tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt
    37561 taaaagcggc ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag
    37621 atcgtttgat tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg
    37681 atttacctac attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag
    37741 aaacctggat tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa
    37801 ttcgccagtc ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg
    37861 gtggcgatgc cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa
    37921 tttccggaca taagagccgt tttgccattc gttttatgcc gctggacact gaaaacggac
    37981 aggtaccgga acgtctggat ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa
    38041 ctattacggt gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt
    38101 ttcggccatt ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag
    38161 aaaaacgaat ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa
    38221 agcagtgaca tttgtctgat gcctcacgta ggcctgataa gacgcggaca gcgtcgcatc
    38281 aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt atccgaccta cagacgctta
    38341 agcttcgcaa ttaccggttc attggctggc ggaaaatcat cggcattaag accgaccagc
    38401 gacatccact caccgggttg cccctcttta ccccacggca ccccttccca actttcgacc
    38461 agccagaacc acagagttat atgcctgtcc gggaattcat attccagttt ttcaaatagc
    38521 gaaaaatgtt gtggggtaat cccgacctct tcctgaagtt cacgcaccac cgcctgttcc
    38581 ggcgtttcgc ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca
    38641 tctgctgcgc gacgtgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca
    38701 atttgcagct ttttcattgt cttaaaccta taaaaaaggc gcagaatcct gcgcctttta
    38761 cttcaactgt tagcttttat tgcaggcgac catggcactg cttgtatttt ttaccagaac
    38821 cgcacgggca aggatcgtta cgtcctactt tgcgttcacc ggtttgcgcc gccagtgcag
    38881 ctgcggctgc agagtcgtca tcctgatggc taagctgctg catttgtgct aaacgctcgg
    38941 cttccatacg acgctgttgt tccagctcct caacctcttc aggcatacgt acctgaactt
    39001 tgctcagcgt actgataact tcatatttca acgactccag catcgctgca aacatggaga
    39061 acgattcacg tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac
    39121 cctgacgcag atagtccatc gctgccaggt gctctttcca cagggagtca agcgtttgca
    39181 gcatgacgcc tttctcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct
    39241 gatacacttc gatggactgc gccagaatgc gctcacgcag cgtctcttca tgcagttctg
    39301 gttctttatc cagccactcg gcaattggta aatcgaggtc gaaatcgttc ttcagacgtt
    39361 cctgcagccc cggaatatcc cacatttctt ccagcgactg tggcggaatg taggcatcaa
    39421 tggtggcttt gaacacatct tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat
    39481 ccagcagttc gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat
    39541 attccagcag ttgcttacga atgtcgaagt tacgactttc aactttacgc tgggcgttgg
    39601 caatcgcttt agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac
    39661 gcatcatgcc ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca
    39721 ggtagaaacg ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat
    39781 cgatacgacg ggattcgtga cgctcggtac cgatgatatg caggccacct gcttccagta
    39841 ccgcatcgtg acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat
    39901 tttccagcgc ggcaacttct gcctgccagc tacctccgag cacaatatct gtaccacgac
    39961 ccgccatgtt ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg
    40021 cttcgttggc gtggaatttg gcgttcagga cattgtgctt aataccggct ttggtcagtt
    40081 cgtttgacac cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt
    40141 tcgcagtacg ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt
    40201 agactaggtc cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat
    40261 ccagtttgta gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg
    40321 ccagtttttc atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt
    40381 cgttctggat ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc
    40441 gtcggccctg catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa
    40501 cgatgtagtc gacgtcacgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt
    40561 gcatcagcat gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt
    40621 tcaccagcag ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt
    40681 tttcgtccac cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac
    40741 ggatcaggtg cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg
    40801 ggccggaaat gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca
    40861 ccagcgcata gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atgttgtcgc
    40921 gcaggtagtc aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt
    40981 cgcgctttgc cggtgctggc atgcccggca ggttgatacc aacagtcagg ccaaggaatt
    41041 caaacagcgg acggttgttt tcggcgtcac gttgcgccag gtagtcgttg acggtaacta
    41101 cgtgcacgcc tttaccggtt agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt
    41161 taccttcacc ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta
    41221 actgaacgtc gaagtgacgc ataccaaaga cgcgcttact tgcctcacgc accacggcga
    41281 aagcttccgg gatcagattt tccagcactt cgcctttttc cagacgcgca cgaaactctg
    41341 cggttttccc tttcagttct tcatcggaga gtttttccat ctccggttcc atggcattga
    41401 tgatgttgac cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt
    41461 tagttaacaa tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa
    41521 aatgaagtaa aggtttattg ttgttaggtg aggcgttgag ggccagcacg gatgccttgc
    41581 gcctggctta tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca
    41641 atgcgataac ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc
    41701 agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagtgtttgc
    41761 ggtgccattg cgaaagaaag atgacggatt accgtgcgaa tagcatgttg atgccagtaa
    41821 tcaacggaat agttcgaatt cgggcggcgc gtgttcgctt ccagcaaggc caattgacca
    41881 aagttaactt tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt
    41941 tcggcggcgt tgctgagcgc aggcaaacct aaactcgccg cgaccatccc taataagaga
    42001 tgcggccaga agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta
    42061 ttgccatccc gatcgaattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgaa
    42121 gtccacagca cgaatgaaga taagttgtgc gtaaaagtgc ttaagaaaac agcgttcgca
    42181 ccgtttttat agctcttata aaattcaaaa aaaaacgact ggttcaccta gccggagaga
    42241 gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg ccagtacagc
    42301 tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca catattccca
    42361 ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat gaccggattt
    42421 ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac cgatcgcatc
    42481 gagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg
    42541 ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac cacgggactg
    42601 cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa
    42661 cgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag ccggatggtt
    42721 aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag cccacttatc
    42781 gccatcttcg acacgaacag tctctttgat gcgaacaaat tttttggcgc agttcaactc
    42841 gtcgataccg gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg
    42901 gatttccggc gcgttaactt cgataacaat gttatcgatg cccaggcccg cgagagcagc
    42961 attgaggtgc tctacggttg aaatccgtac atcatgctcg ttgaccagac acgtacagag
    43021 catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat tcaagtcggt
    43081 gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg tgactttctt
    43141 gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat
    43201 catcgtatta tctcgccaaa ttacctatcc aaccgaagtg tactatacat tcggcgggcc
    43261 agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac
    43321 gtaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc gcattgtcat
    43381 tcactacttt agcaaccggc ttctgctcct gggtcagcgg agccatccca tgctgctggt
    43441 agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt tcaggacgtt
    43501 tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag ctcgtcattc atatccgggt
    43561 caagagaagt accgataacc acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac
    43621 ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc cgtgatgtta accagcacgc
    43681 cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc atttcagcag
    43741 cttcttccgc acggtcttca ccgctcgcca cgccagaacc catcattgcg tagcccattt
    43801 cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga cgagtaatca
    43861 gttcggcgat accttgcaca gcgcctttca gtacatcgtt cgctgcgcca aacgcatcca
    43921 gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata gtgatcagag
    43981 agtccacatg cttggacagt tcagtgatcc cctgctcggc gaatgccata cgcttcttgc
    44041 cttcaaagtt gaaaggctta gtgacgacag caacggtcag gatacccaaa tcttttgcca
    44101 cttcagcgac gactggtgct gcacctgtac cggtaccacc acccataccc gcagcaataa
    44161 agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc gcgatcctca tcagccgcat
    44221 tgcggccaac ttctggatta gcgccagcgc ccagtccttt ggtgataccg ctaccgattt
    44281 gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt accgcgaaga
    44341 attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc
    44401 cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca aacatagttt
    44461 ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat aaaaattaaa
    44521 actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat gctgtaacac
    44581 gtttttctac ttcagcttca ccgttaagat gtgactcttt cccatagtga agcaatccca
    44641 ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaatg ttcagcggcg
    44701 cgccgatacg cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct
    44761 gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc ccttgttggc
    44821 gaagcttttc ctgcaactgc aatatctctt cgttgaccag gttgagcaac tcggtatagc
    44881 gcggctcgat cacctctgcc agggtctgac gttgcagact ccgcggcgga cgaccgccta
    44941 cgctcggcac ttccacgctc tcatcttttc caacgatgga acccagcgca caaccgtggc
    45001 gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg
    45061 tcacgacatt gccagcataa ggaattacct tagtgtggcg caatgcccca ccggtataaa
    45121 cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt tcacgttcat
    45181 cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg tcaactttca
    45241 gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga catgtgatca
    45301 ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac cggattcttg atcccttcct
    45361 gatagtcaat cgcatactct tgcgggatca catgcagcac acgatgctca tcgcgcacac
    45421 gcaccgattt cgcggtatgg acgacatttt ccacatcttc ttgcgtcact tcttcttcag
    45481 aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa agcgccagat
    45541 ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg cgttgtacgc
    45601 acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgt gacgggcagc
    45661 tgcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact aaagcggcaa
    45721 ccttcgcggt accaatttcc agtcctacta ccagttttct gtccgtcgcc ttgatcattg
    45781 ttgttctgcc tgtgcctgat tttgttgctg agtagattcc tctggcggca agggcgccca
    45841 gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt tgccatcggt
    45901 ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac gtttcatcgt
    45961 atcgccccgg ccaagattga gcttaatatc gttattcagc gtcaactgcc aggaacgccg
    46021 cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct gccccatttc
    46081 gcgatagccc tgcaacactt cattagcgct gccttccggg ccatacagca ttggaagcac
    46141 ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt ctaccatgtg
    46201 ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca attcatcagg
    46261 ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta tttgcgtctg
    46321 gatgatgttg acatcctggg tcataaaggt acccggctca cccaatgcca ggatcgactg
    46381 ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg
    46441 caggcgttgc gcatcttcca tccagctcaa cacgacccag ccgctcacca acactgtcgt
    46501 taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc ggcgagaaga
    46561 aacctcttct tcgctgtttc gcgtgttcag agcagcctgc gacatattag tccgccagtt
    46621 ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca
    46681 ccaggctgtg gctggtcata cccggtgagg tattggcttc cagcaaataa aactgtccat
    46741 cgctgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc gtccatgctt
    46801 tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg gggcagaaat
    46861 actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg gacggttgaa
    46921 tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc ggcccactta
    46981 gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat gcatcttgta
    47041 gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg ctcggcttaa
    47101 cgataaccgg caaacccaga gcagaaattt ctgctaactg cttatcgctc aggccttttt
    47161 caaactctgc gcgggttaat gctacccacg gcgcgaccgg taaaccggca ccttgccaca
    47221 gaagtttgct gcgtagttta tctattgaaa gcgcagatgc catcactccg ctgccggtat
    47281 aaggcaagcc catcagctcg agcatgccct gcagcgtacc atcttcaccg ccgcgaccgt
    47341 gtagcgcgat aaacactttc tgaaaaccca tcgacttcag ttgcgtcacg tcgacttctt
    47401 tcgggtcgac aggatacgca tcaataccgc cttcacgcag cccggctaac accgctgcgc
    47461 cagaattcag agaaacttcc cgctcagcgg aagtcccgcc caacaggacc gcgattttat
    47521 cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc taaagaacgg
    47581 gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc ggttaatacc
    47641 ggtgccagca tctcggctac ctgcgcaggg tccggcacca gaatgggatc aattttccca
    47701 cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca
    47761 gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt ggcgaaatca
    47821 tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac caggttttta
    47881 tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa cttccgtcgg gtggtggccg
    47941 tagtcatcga ccagcattgc cgtaccgctt ttaccattca ctggctccag cgggaattca
    48001 ccgaggaaat caaaacggcg accagtcccc tggaagctct ccagcgcacg caaaatagcc
    48061 tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta
    48121 tgacggcctg gcgcattcag ggtgacgcgc atcggctctt tgtcctgacg cagcagcgta
    48181 aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc atcttcgctg
    48241 aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca attcgcggat caccggatca
    48301 tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt gcagaaaatt aataaaggtc
    48361 tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc gatattggtg
    48421 acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc tgcttcggca
    48481 atcaggtacc gaccatgccc caaacgcgca tgaacccccg ccgcttttac cagcccgccg
    48541 ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc tggaaaccat cgcggtagtt
    48601 gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac gaaaacgcat taactcagcc
    48661 agcatttcgg cacgacggat caccggaata cgcgcttcat gagctgcgac aatttccggg
    48721 ttgtcggcag aaatggcgct ggaaacaacg accacgctgg catcacgtac gttttccggg
    48781 cgatggttga aataaatcgt cgcacccaga ttcagtaact gctgcgtgac cggatttggc
    48841 gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc aataccgccc
    48901 ataccggcac caccaatgcc gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg
    48961 atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa cttcttaaat
    49021 tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc acttcatttg
    49081 ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc atggttaata
    49141 aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta agctgtggct
    49201 gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag tattgctggc
    49261 ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg atttcactca
    49321 cagttaaagc accggagcgg caaacgacga catccgccca cgcatacgcc gccgccatat
    49381 catcaataaa ttcagtcact ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa
    49441 cggattgttg cgaacctttg ccgctctgat gccagatagt gactgaatca cccagtttcg
    49501 cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca cccactacca
    49561 acacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc agcgccagca
    49621 catcggtacg caccgggtta cccaccactt ccgcattagg gaaagcgcct ggaaacgcct
    49681 gcatcacttt ggtagcaatc ttcgccagcc atttattggt taagcccgca ataccgtttt
    49741 gttcatgaag tacaaccgga atgcctaacg accacgcggc cagaccacct ggacctgata
    49801 catagccgcc cataccgagt accacgtcag gtttgtacgc tttcataatc gcccgcgcct
    49861 gacgccaggc gttgaagata cgcagcggcg cagctatcag tgcttttata ccttttccac
    49921 gcaaaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact aagtccgctt
    49981 ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt agatagtgcg
    50041 caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc attaatcgct
    50101 ttccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca gacgcgtttc
    50161 ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc tcgaaccacc
    50221 gtaactgatt agcggcaatg tcagaccttt ggtcggtaac atccccgccg ccgcgcctac
    50281 gttaaccagc gcctggaagc taaaccagat gccaatagaa caggcgagaa aaccggaaaa
    50341 acggtggtca atttctaatg ctttgcggcc aatcgacatc gcacgaaaag cgacgaagaa
    50401 taccattaaa agtgctagta ccacaccgac ataacctaat tcttcgccga taatggcgaa
    50461 aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgaat tacctaaacc
    50521 ttgcccccaa agttcgccgc gaccaaacgc catcagcgat tgcgttaact gatagccgct
    50581 gccaaaggga tcttcccacg ggttccagaa tgcggtaaca cggcggatac ggtacggttc
    50641 ggcgaggatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa tgaactgcca
    50701 caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca ccaccaccgt
    50761 accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaatcacgc ccatcggttt
    50821 caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat agttggcgat
    50881 atagcaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca aaccgagatc
    50941 gatccaacgc gatgcccctt taacagcgct acccactacc agcacgatca tcagcaggat
    51001 gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg gcagacgcag
    51061 cgtaatgatc gccagaataa acgccaaaat cagatagaca ccatcacgct tcgcgaagaa
    51121 gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca ccatgataaa
    51181 gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct
    51241 gtcggtatct ttttcccgcg agcccatcac ccagcccttt agcgccgtgg agatccagac
    51301 caggatactg aatcctggca ggcgcggcat tttcaggcga gggagagata aacgcatcaa
    51361 cctaactcct tcgccagaca ggcaaactca ttgcctcgtt gttcaaagtt cttgaactga
    51421 tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac acgcggagcc
    51481 agcaagcgca tagcctgttc catagtttcg gtttgttctg ccacttccgg gcgtagcgcc
    51541 gccagctgcg cgccgtcacg accgaaacaa tacagacgta cgttatcgcc attcaggtaa
    51601 cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc ccagcaacaa atgcagcgtg
    51661 ccgtctacgt gcaggccatt cagcgccgct tccgtactgc cgacgttggt cgcttttgaa
    51721 tcgttaatcc agcgcacacc gttatgctcc agcaccactt caaagcgatg cggcagaccg
    51781 gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta accctgcggc atctgccagc
    51841 gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat ctctttcaca
    51901 ttcagcactt tctcgccttt aacccgcagc caggtttcgc cctgctgatg attcaggtga
    51961 tagtcaccca tgttgacgcc aaagctgacg cagcgttcat ccgcaccgcg aatcggcatt
    52021 gttaaggcat catcagcatt aaccacgcaa actttcgcgt tttcgtaaat gcgcagtttt
    52081 gctgcacgat actgttgtaa accaaacggg tagcgatcca tatgatcttc agtcacgttc
    52141 agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt ccagctggaa gctcgacagt
    52201 tccagcacgt acagttcaca ctcatcatcc agtagcatca acgcaggcag gccaatattg
    52261 ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag cgtggtgacc
    52321 gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc gcgacaaaac
    52381 agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc
    52441 gccagtgcaa taccgggact ggcgacaatc agatctgccg ccatcagcca ttcatcattc
    52501 agactgcccg tgtggcgttc tacggcttcg ggtaatttat ccaggccagg cggtgtcata
    52561 cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac gcaggaaagc
    52621 ccggtgaggc ccaggccgat aatgacaaca tttttaccct ggtaatcagc catgattaac
    52681 gtaccttcag cgttgccaga ccaatcagga ccagcatcag cgaaataatc cagaaacgca
    52741 caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc
    52801 ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgaca gaaagcgttt
    52861 ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgtagc agtacggcga
    52921 taatgcctaa cgcaccacct aacgccagcg aaccgacatc gcccataaag acctgcgccg
    52981 gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgcggta cagacaataa
    53041 ccagttcccc ggcgtgtcgc agatacggta tatgtaaata gctggcaaag ttcatgttgc
    53101 cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca
    53161 gaccgtcaag accatcggtc aggtttaccg cgttgccagt acccacaatg acgaagtaag
    53221 ccagcagaat gtagaacagc cccagttgcg gcatcacatc tttaaagaat gggaccacca
    53281 gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc gaaagcaaca cccagcgcaa
    53341 tgaccgacat ccagaaatat ttccaacgag cgatcaaccc tttggtgtct ttacgcacca
    53401 ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc accaacacgc
    53461 accagacgta cggattggac gggtaagccc acagcagcac ggagatcaca atcgctgtca
    53521 ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt
    53581 cgttacgcac tacctgacca aaggaaagtt tttgcaaatg agcaatcatg cgcgggccca
    53641 tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg
    53701 aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt
    53761 cccattctcc tgtaaagcgc gtactacctc ttccatggca gcactacgtg aacccttaac
    53821 taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgtaa ttaacgcagt
    53881 tttatcagca aaatgttcgc caacgccgct ggcggtgctg atagcatggc tttgtttacc
    53941 catgcttaac acgcggtcaa taccagcagc ttttgccgcc tcgcccacct gtacatggca
    54001 ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc ggtagcccgg
    54061 catttcagcc agtacctgaa cagctgcggt cattgaaccg acattggcgt tgtaggagtc
    54121 gtcgagcagc aactggtttt ctgccagttg gatggggaac agacggccag gaacagcttt
    54181 cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc
    54241 agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca gaacatcgac
    54301 gctaccggtt ggggtttgta gggtaaattc cataccgtgc gaggtcacat ggatattggt
    54361 ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc cacactttgc gtgagccaat
    54421 tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga taccgttttc
    54481 cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc
    54541 ttccagatgc gccgctgcca ggttgttgac cagcgccgct tccgggcgag tcagactcac
    54601 agtccaggca atctcgccct gatggttcgc gccaagttca ataactgcgt aatcgtattc
    54661 cggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat tgcctgccgt
    54721 ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc atctctttaa cggaggtttt
    54781 gccggaggac cccgtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc
    54841 cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcaggt cgatgtccag
    54901 cggacggcta accagtagtg cgcctgcgcc gccagctttc gcctggtcgg caaaatcgtg
    54961 ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag cccggcgtca gttttcgggt
    55021 atcagtggtt acagcatcaa gggtgatatc tgcaccttgc agttcaccgt tgagaatatc
    55081 ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg
    55141 acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc atggcctttg
    55201 cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt caccgcttca
    55261 gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag aatatcgttg
    55321 atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca
    55381 aactcttcgg caattgcgcc catcagtgga cgcttacctt tatcgcgatc gccaccacag
    55441 ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc
    55501 agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttacctgg cgcagtgaac
    55561 acttccatac gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcag atcagccagt
    55621 ggatagccca gcgccagcag tgttgccagc gcgagcaaca ggttgctgac gttaaaagcg
    55681 cccatcagat ggctttcaat ttcgccatcg ccccagcttg aactaaagcg aatggtcgcg
    55741 ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc gtccgtgaca gttcggattg
    55801 atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc
    55861 acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gttcagaata aagcagccat
    55921 ttcgcagctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg
    55981 gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag cccgtgggag
    56041 gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag ctcatgctga
    56101 acatcgactg ccgaaccggt agtattttct gtcgggatca ctttccccag cagaccgtta
    56161 ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccagaagc
    56221 tgggtcgtcg tggttttgcc gttggtgccc gttacgccta cgagacgtaa attgtcagag
    56281 ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag
    56341 atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct
    56401 gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc
    56461 tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc
    56521 atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa
    56581 ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt
    56641 tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga tggtaccaaa
    56701 gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac
    56761 aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt
    56821 attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa tggcgatacg
    56881 atagccttta atcgccgcct tcacgccgcc gccgcctggt agcgccacgc tttccatcat
    56941 atgcaccacg gtgcgaacaa tggattccgg gaagacacgt tcaccgggaa ccggggggtc
    57001 aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg
    57061 cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat
    57121 gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt
    57181 ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc
    57241 taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa
    57301 ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag gaatggtatt
    57361 gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcatcacca ccatcggttt
    57421 aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg cctctttcgg
    57481 cgtgccgctc agattgttag ggttgtatga cgggctgtta gccatcgcca gcacttcacc
    57541 tgtgttgaca tccaccagca cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt
    57601 gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg
    57661 cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc ggtctttacg
    57721 cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct caacgccctc
    57781 aatcccttga ctatcgacgt tagtaaagcc gatgaggtga gcagtcactt cgccggacgg
    57841 atagtaacgg cgagactctt cacgcagatg aatccccggc agtttcagtt ttttgatgta
    57901 gtccgccatg tcagggttta cctgacgcgc cagataaata aagcgccctt tcgggttggc
    57961 gttaatgcgg gcagaaagct gatccagcgg aatattgagc gcgttagcca gcgccttcca
    58021 gcggtcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc
    58081 ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga
    58141 ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca gcatatccgg
    58201 ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca
    58261 gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg
    58321 tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat
    58381 attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac
    58441 ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa
    58501 agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc
    58561 cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt
    58621 cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc
    58681 catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc
    58741 tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc ttcttcgccc
    58801 ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag ctgctcttca
    58861 gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc
    58921 acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc
    58981 acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg
    59041 cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgca
    59101 gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg
    59161 acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag
    59221 gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg
    59281 tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgttc agcatcatca
    59341 agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc aataagatcg
    59401 cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga
    59461 tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc
    59521 ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca
    59581 tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc
    59641 gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa
    59701 gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt
    59761 ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc
    59821 ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc
    59881 gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag
    59941 acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg
    60001 gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgttcccg
    60061 ataacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg
    60121 gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt
    60181 ttacggagcg taggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc
    60241 gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac
    60301 gttatgaata gctgtaagaa aaaatccctc atcgtcatca ctgttttaaa atgactcact
    60361 attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc
    60421 ttccccggtt aacagtcctt gttacgtctg tgtggcgctt gatgccagcg ggtttgcgca
    60481 aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc cgcatgtaac aaaataatgc
    60541 aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc agctggtgag ttaattaacg
    60601 ggcgggttta aaaccattta tcaataatag tcaggaatag tcttatttac tttaaggata
    60661 ttgatgtcca gtcccgtact ctacgcgcca gagggaaatt cacctggcgc gtatttttgt
    60721 tcgcggctta gctacggctg agcacgccgc ggcgatagag attacgttta atgcgcgtta
    60781 aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc agcacacgct
    60841 ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg acactgtaag aagtcgagca
    60901 gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttgccg tcgcgacgca
    60961 gcgtgacatc catcactcct tgcaacaacg caaacgacgt tgtgaacagc gcctgcggca
    61021 tcggatgcgt ttccagccat ttttcgaata actgggcagc cgcctcccgc tcatagctgt
    61081 tggcatacag gaaatgcact tcgcgcggat catctttcca agcagtacgg aaaccttgtt
    61141 cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagcacc gtctcggcgg
    61201 gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca ccaaccacgc
    61261 tggtgaaatg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac gggtcgttag
    61321 cccagcgttg ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata atggcatcaa
    61381 cctgacgctg taaaaggtgc tcaatgcagc gcatttcgtt gtctggctga tcttctgagc
    61441 aggcaatcag cagttgataa ccccgttgcc gcgcctggcg ttcaagatag ttagcgatgc
    61501 gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt gtgcgtccag
    61561 cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc acagccatga
    61621 ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta ataacatagc
    61681 ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc acaattgccc
    61741 cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt cattaacatc
    61801 taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact gatttcgcaa
    61861 aaaaagccca acgtgacatg ttgggctgca aattgcgcac tgagatcatt ctcaacgcat
    61921 tattttatcg ccgcgcgaaa gtccgaccac accagagcga gcaacttcca caattttcgc
    61981 cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac
    62041 ggtataaagc gagggggtga catcgataat ttgcccacgg aatatttccg tattacgttt
    62101 cacttcgtca cgcccgtaac cgctggcctg aattttcacc agcatgattt cccgctcaac
    62161 attcgcgccc tgccccaact cactcacgcg caagacatcg accagcttgt gtaattgctt
    62221 ttcgatctgc tcaagtactt tttcatcgcc cacggtctgg atggtcatac gcgataatgt
    62281 cggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagcctcgct gggaaaaaag
    62341 gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata atatccggcg
    62401 cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc cccgcgaatc
    62461 tgcatcgggt agacgtgctc actgccatcg acggtaacat caacaaacac caggcgatta
    62521 ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca gctcatgcgg atgagagatc
    62581 tggatcccga cgtgaccata ggcttccgcc agacggacga aatcgggtag tgattgcata
    62641 taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgtttcac catccccaga
    62701 tagcggttat tgagattcac caccagtacg ggcaactcgt attgcaacgc ggtagacagt
    62761 tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt ttcttctggc
    62821 aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc gagaccgccg
    62881 gaattgatcc agcgacgcgg tttgtcgaat ggataataaa gcgcagcaaa catctggtgc
    62941 tgcccgacat cggacgtcac gtaagcgtcc ccattagtca accgccaaag agtctcgatc
    63001 accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg aagagcgcgc
    63061 cactgttcaa tctgctgcca ccagtcgcgg atctcatcca gcggttgatg ggcggattct
    63121 tgcgacagga gttcaagcat ttgttcgagg acctggcgag catcccccac aatcgggata
    63181 tccgcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag aacagtggca
    63241 tttgggcagt actttgccag attgttcgtc gttcggtcat caaatcgtac cccgacggcg
    63301 aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg cattcccagc
    63361 attcccagtg cctgacgatg cgttgccgga aacgccccca gccccatcaa tgaggaaaca
    63421 acgggcagat tcaacgcctc caccgtttct ttcaactgct gatggcagcc cgccatgatt
    63481 gccccaccgc ctacgtagac aaccggtttt tttgccgcta ccagcgtttg cagagcacgc
    63541 ttaatttgcc ctttatgtcc ggtagtagtg ggattgtaag aacgcatact gaccgactca
    63601 gggcagacat agggtaattt gttcgccgga ttaagaatat ctttcggtaa atcaacgacc
    63661 actggtcctg ggcgaccact tgccgccagc cagaaagcct ttttcagcac ctgcggaatg
    63721 tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg gtcgcgaaat ccccaccatg
    63781 tcgcactcct gaaaggcatc gtaacctatc aacgaggtcg ctacctgccc ggaaaggaca
    63841 actaatggaa tggaatccat ataagcggtg gcgatgccgg taatcgcatt ggtcgcccct
    63901 ggacccgacg ttaccagcac gacgccgact tccccggtcg cacgcgccag gccatcggcc
    63961 atatgcaccg ccgcctgctc atgacgaact aatacatgat caataccacc cacggtatgc
    64021 aatgcatcgt aaatatcaag gactgcgcct ccgggataac cgaatacttg tttaacgccc
    64081 tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat ggcctgcctc
    64141 actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct taagcaattg
    64201 gcagccagag acataaacgc agggtaataa gaataaacag gaggaaaggt gaaaagaaac
    64261 gataaaatcc tccattacga aggattaaat tgataataaa tcggctgaat cccacaactt
    64321 acagcaattt ctctattttt ttaattaatc ctaaaataga gagtccacac attcagaata
    64381 aaattctaca ttcaataaaa aatgcagaat aaaccagaca ttcatgtctg gcctattctg
    64441 cattcagtta gcgtttgcaa attgagacta attgttcttc catccactga tggcctttat
    64501 cgcgcccggc agcttcatgc caggagagat aacaggttct gctgttttgt tttaacggca
    64561 gcggtaatac ctgtaattct aaggattcag cgaactcttc agccagccaa cgcggcgcaa
    64621 tagcgaccaa atgcgtttgc gacaccacgc taagtacgct catcattgcc atgccctgat
    64681 acgcgatact ggcttgctta tctaccgtgt cataccaagg ttgactaaat gacgcgaaac
    64741 gatcgagcga aaccgccgca tgttgttcgt tataaacatc atgtttcagt aacgggcctt
    64801 taattgttgg atgatttttg ctggctacca gcaccatttc atctttaaat aatggcacgc
    64861 tggtaaattc aggacgatgg aagtcttcat aactaatcac aaactccgtt tcctgataac
    64921 gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt atatttggcg
    64981 caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac gggctgcaaa
    65041 cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacta ggcaattcat
    65101 tttgtactag ttgcaatgcc tggcgaactg aaccaaaaag ttgaaatgcg cgagcagtcg
    65161 gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc ttcaggcgtg
    65221 caacagcgtt actgaccgca ggttgcgaca tacccagaac atgagcggca cgggtaatgt
    65281 tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc atgcgtagct
    65341 gtggcttgct taactccgcc gtctctgaat gatctgtttg tacctctggc atacttaact
    65401 ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat cctgaagaat
    65461 atgatttatc atgcatataa aataagaaaa agcaaaatga gtaaaattcg gaaaacataa
    65521 agatacgggt tctgataaaa acagaatcca ttgccgtgga atgagtcatt taccacagca
    65581 taataatcca taatgttttt gttgcgaaaa caatctaatc ataactaccg cgaatactca
    65641 atcatctaca aaatggatta aatgtgctta tttaataatt aatttatata tttaatgcat
    65701 taattcttaa cattaattga tcaataatat tcaccaaatc aatatcaaaa aaaatcgcaa
    65761 aacatataat tcaatacaaa tcatcaggat aggttttgca acgcgtgcat tttgtcccct
    65821 ttttcctcgt tgattagatg caaaaattta tgctgaaata tgtcaaccga tgaaaagcgt
    65881 cggtagttaa gcagaaatta atatcgctta ctttaaccac cgtagcacaa ttagctaatt
    65941 ttacggatgc agaactcacg ctggcgggac gtttttattg cgtcagggtt gacatccgtt
    66001 tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac
    66061 atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg
    66121 agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg
    66181 tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc atgagccagc
    66241 aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga
    66301 gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg
    66361 aagtcggttt ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc atcgcccgcc
    66421 aggttaaaaa cagccgcgta tgtgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg
    66481 cggccgaatc cctgaaagtc gccgaagcct tccgtattca tacctttatt gctacctccc
    66541 caatgcacat cgccaccaag ctgcgcagca ccctggacga ggtgatcgaa cgcgctatct
    66601 atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg
    66661 ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca
    66721 ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca
    66781 tcagcggcct gtatgaacgc gtacctaaca tcgacaaagc cattatttcc gtacataccc
    66841 acgacgattt aggcctggcg gtcggcaact cactggcggc ggtacatgcc ggtgcacgcc
    66901 aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcg ctggaagaag
    66961 tcatcatggc gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc
    67021 aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa
    67081 acaaagccat tgttggcagc ggcgcattcg ctcactcctc cggtatccac caggatggcg
    67141 tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa
    67201 tccagctgaa tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga
    67261 tggggtataa agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg
    67321 cggacaaaaa aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc
    67381 agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg
    67441 atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca
    67501 acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg
    67561 tcgaactggt gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg ctgggtcagg
    67621 tggatattgt cgccaactac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata
    67681 ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag
    67741 aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt
    67801 gatgtcgaag aattaccata ttgccgtatt gccgggggac ggtattggtc cggaagtgat
    67861 gacccaggcg ctgaaagtgc tgaatgccgt gcgcaaccgc tttgcgatgc gcatcaccac
    67921 cagccattac gatgtaggcg gcgcagccat tgataaccac gggcaaccac tgccgcctgc
    67981 gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gcggcccgaa
    68041 gtgggaacat ttaccaccag accagcaacc agaacgcggc gcgctgctgc cgctgcgtaa
    68101 gcacttcaaa ttattcagca acctgcgccc ggcaaaactg tatcaggggc tggaagcatt
    68161 ctgtccgctg cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg tgcgcgaact
    68221 gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa
    68281 agcgtttgat accgaggtgt atcaccgttt tgagatcgag cgtattgccc gcatcgcgtt
    68341 tgaatctgct cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta acgtgctgca
    68401 atcctctatt ttatggcgtg aaatcgtcaa cgagatcgcc acggaatacc cggatgtcga
    68461 actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt
    68521 tgacgttctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat
    68581 cactggctcg atgggcatgt tgccttccgc cagcctgaac gagcaaggtt ttggactgta
    68641 tgaaccggcg ggcggctcgg caccagatat cgcaggcaaa aacatcgcca acccgattgc
    68701 acaaatcctg tcgctggcac tgctgctgcg ttacagcctg gatgccgatg atgcggcttc
    68761 cgccattgaa cgcgccatta accgtgcatt agaagaaggc attcgcaccg gggatttagc
    68821 ccgtggcgct gccgccgtta gtaccgatga aatgggcgat atcattgccc gctatgtggc
    68881 agaaggggtg taatcatggc taaaacgtta tacgaaaaat tattcgacgc tcacgtagtg
    68941 tacgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctagt gcatgaagtg
    69001 acctcaccgc aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggc
    69061 aaaaccttcg ccactatgga tcacaacgtc tctacccaga ccaaagacat taatgcctgt
    69121 ggtgaaatgg cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga atttggcgtc
    69181 gagctgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag
    69241 ggcgtcacct tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc
    69301 gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaaca
    69361 caaaccctga aacagggccg tgcgaagatc atgaaaattg aagtccaggg caaagccgcg
    69421 ccgggcatta ccgcaaaaga tatcgtgctg gcaattatcg gtaaaactgg cagcgcaggc
    69481 ggcaccgggc atgtggtgga gttttgcggc gaagcaattc gtgatttaag catggaaggt
    69541 cgtatgaccc tgtgcaatat ggcgatcgaa atgggcgcaa aagccggtct ggttgctcct
    69601 gacgaaacca cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc
    69661 gacgacgccg ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc
    69721 gttgtcactc tgcaagcaga agaaatttca ccgcaggtca cctggggcac caatcccggc
    69781 caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa
    69841 cgtgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggtat tccgctgacc
    69901 gaagtggcta tcgacaaagt gtttatcggt tcctgtacca actcgcgcat tgaagattta
    69961 cgcgcggcag cggagatcgc caaagggcga aaagtcgcgc caggcgtgca ggcactggtg
    70021 gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga taaaatattt
    70081 attgaagccg gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac
    70141 gaccgtctga atccgggcga acgttgtgca tccaccagca accgtaactt tgaaggccgc
    70201 caggggcgcg gcgggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg
    70261 accggacatt tcgccgacat tcgcaacatt aaataaggag cacaccatgg cagagaaatt
    70321 tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat
    70381 catcccgaaa cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc atctgtttaa
    70441 cgactggcgt tttctcgatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt
    70501 cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct gtggctcctc
    70561 gcgtgagcac gcgccctggg cattgaccga ctacggtttt aaagtggtga ttgcgccgag
    70621 ttttgctgac atcttctacg gcaatagctt taacaaccag ctgctgccgg tgaaattaag
    70681 cgatgcagaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga tccatttcga
    70741 cgtggatctg gaagcgcaag aggtgaaagc gggagagaaa acctatcgct ttaccatcga
    70801 tgccttccgc cgccactgca tgatgaatgg tctggacagc atcggcctga ccctgcagca
    70861 cgacgacgct attgcgtctt atgaagcaaa acaacctgcg tttatgaatt agaccccttg
    70921 cccggtcaaa tgaccgggct ttccgctatc gtccacgtca tcaaacgtct ttaacctttg
    70981 cggttaaaaa taatgcgaca acagaaataa tcgcaattac ccagtagaca gcaaagtgcc
    71041 cccaactttg tgcaattgct ccctgaataa caccagccag aattaccccg gtagagatag
    71101 tgttggtaaa taaggtggta gctgcccccg ctcttccagg cattaaatcc tgaaaccata
    71161 gcatcccaat acccgcaaca atgccgataa atacagcgtt aaaaagttgc agcgtcatca
    71221 acgccatacg gctatgaaag aaaatcaatc cagtgtaaaa cagcactcct gccaccactg
    71281 caatgaccat cattcgccgc ttaccataac gtttgacata gtagccagcc agaatcattg
    71341 ctggtatttc cagtccagct gccgtcccca tcaggaaacc cgccagtttg tctggcaatc
    71401 ctaaatcgct actgatccac aacggcatat caataatgta catagtgttg caggtccaca
    71461 ttaacgtcga ggcgacaaat aacatccgta cgttactatc ctgccagtca ccttgcattg
    71521 ataaagcatt ttccgacggc agttctaccc gcgccacaga cggaagcata aatgcaatca
    71581 ataccagact gagtgtgaat atcccggcgg caatcgaaaa catcactgta aagccgtaat
    71641 tcaacgccag cataaaggcc agcggtggac cgattaccca tgccagagaa agctgcgcac
    71701 gcatcaccga gctaaacatc accacttctc gcgccgagtt atccgcgtat tcccgcgcca
    71761 gagcaaataa ctgtggcatt gccgtattgg ccagagatgc cagaagcaca ccacaggtga
    71821 taagcgtcag ataatgacga ttaaatgcaa acagtagcgc attgccaata gccatcaaac
    71881 agcaaaatat aatcagtttt cgccgatcgc cctgactgtc agaacgtttt gccaaccaga
    71941 ggcttacgcc gatcccagca atagcattca ccgtataaaa gagtccgatc cagaaaggtt
    72001 gcgcgccaac ctcacgactc agaaataagc tcaatgtagg agcctgtagc gccccggcca
    72061 cccccatcat aaaagcgacc agcataaatg ccgcgtaaac accgttcata cggcgagcca
    72121 tcgtcattat ccagatcata cgttcccttt ttagcgcggc gagaataaaa aaaaccagca
    72181 ggtataatct gctggcgggt gattttacac caatactcag tcacacatga tgcaggcaag
    72241 tcaactttca gaattgcggt catcccactg catcagtcct tcaagcatgg ttaatcgttg
    72301 tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat agtgctgata
    72361 aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact taatataggg
    72421 aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg ctcgtctgca
    72481 acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa
    72541 cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgtaccctgc tcaacaccat
    72601 gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgcggtaaac gctcgcgtct
    72661 gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc tgctggagca
    72721 ggatcgtatc gaccaactag tgcagttggt tggcgacaaa gcgactgtgc ggcaaatgct
    72781 ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg tgctctacta
    72841 tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa cccatattgc
    72901 ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac tggaagcaga
    72961 catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt tgcgtccagg
    73021 agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct ctttaaaacg
    73081 aatcaatacg ctgccgctct attcgcatat tacggacatt gtgtcgccga cgccctggac
    73141 gctggatatc catctcacgc agccggaccg ctggttgccg ttactgctgg ggcaagttcc
    73201 ggcgatgatc ctgccgtgcg aatgggaaac cctcagtaac tttgccagcc atcctattgg
    73261 caccggtccg tatgcggtga ttcgcaacag caccaatcaa ctgaaaattc aggcattcga
    73321 tgacttcttc ggttaccggg cattaatcga cgaagttaac gtctgggttc tgccggaaat
    73381 tgccgacgag ccagccggtg gtctgatgct aaaaggtcca cagggcgagg aaaaagagat
    73441 tgaaagccgc ctggaggaag gttgctacta tttactgttc gacagccgca cccatcgcgg
    73501 ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta atctggtcta
    73561 tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc tcccccgttg
    73621 gcaccatgcc cgcaccataa agagcgaaaa accggctggc ctggaaagcc tcaccctgac
    73681 cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc agattctggc
    73741 aagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc atgaaggaga
    73801 gatcgagagc gatatctggc ttaatagcgc caactttacg ctgccgctgg atttttcgct
    73861 gttcgcgcac ttgtgcgaga tgccgctgct ccaacactgt attccgatcg actggcaagc
    73921 cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc agcaactggt
    73981 cgccagtaaa gcaatggtgc cgcttatcca ccactggctg atcattcagg gacaacgcag
    74041 tatgcgcggc ctgcgtatga ataccctcgg ctggttcgat tttaaatcag cgtggtttgc
    74101 gccaccggat ccatgattgc tggtagataa caaaatcact acactaacgc cgttctcaac
    74161 ggggtgccac gcaaacgcgt gcgctgagaa aatacccgtc gaacctgatc cggataacgc
    74221 cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttttg aggtgcaaag
    74281 tgttaaaaaa atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg
    74341 ttctgactgt ttatacctac gattccttcg ccgccgactg ggggccaggt ccaaagatca
    74401 aaaaagcttt tgaagccgac tgtaactgcg aactgaaact ggtggcgctg gaagatggcg
    74461 tttcgcttct caaccgtcta cggatggaag gcaaaaatag taaagccgat gtggtgctgg
    74521 ggctggataa caatctgtta gacgccgcca gtaaaaccgg actatttgcc aaaagcggtg
    74581 tggcagcgga tgccgttaac gttcccggcg gctggaataa tgacactttc gtcccgtttg
    74641 attacggcta tttcgccttc gtctatgaca agaacaaact gaaaaacccg ccgcaaagcc
    74701 tgaaagaatt ggttgagagc gatcagaact ggcgggtgat ttatcaggat ccgcgtacca
    74761 gcacgccggg gctgggtctg ctgttgtgga tgcaaaaagt ctatggcgat gacgccccac
    74821 aagcctggca aaaactggcg aagaaaaccg tcacggtcac caaaggctgg agcgaagcct
    74881 acggcctgtt tttaaaaggt gaaagcaatc tggtactgag ttacaccacc tctccggctt
    74941 atcacattct cgaagagaaa aaagataact acgccgccgc gaactttagc gaaggtcact
    75001 atctgcaggt ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag ctggcgcaaa
    75061 aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc ggtaactgga
    75121 tgtatccggt ggcaaatgtc acgctgcctg ccgggtttga acaattgacc aaaccggcca
    75181 ccacgctgga gttcacacca gctgaagtgg cggcacaacg tcaggcatgg attagcgaat
    75241 ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa gcgccgccac
    75301 gctggtggta gcggttgcgc tcgcggcgtt tctcgccctg tggtggaacg cgccgcaggg
    75361 taactgggtg gcagtctggc aggacagcta cctgtggcat gtggtgcgct tctccttctg
    75421 gcaggcgttt ctctcggcgc tactctctgt cgtacccgcg atattcctcg cccgcgccct
    75481 ctatcgcagg cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat
    75541 cctcccggtg ctagttgctg ttttcggcat tcttagcgtc tatggtcgtc agggctggct
    75601 agcatcgctc tgccaatcgc tcggtctgga gtggaccttt tcgccctacg gcctgcaagg
    75661 cattttgctg gcgcacgtat tttttaatct gccgatggtg agccgcttat tactccaggc
    75721 actggaaaac attcccggcg aacaacgtca gcttgccgcc cagcttggga tgcgcggctg
    75781 gcattttttc cgcttcgtcg aatggccgtg gctacggcga caaatcccgc cggttgccgc
    75841 gcttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcgctgg gcggcggtcc
    75901 gcaggcaacc actatcgagc tggctattta tcaggcgctg agttacgact acgatcctgc
    75961 ccgcgcggcg atgctggcgc tgctccagat ggtgtgttgc cttgggctgg tgctgttgag
    76021 tcagcgacta agtaaggcca ttgcgcccgg caccacgctg ctgcaaggct ggcgcgatcc
    76081 ggacgatcgt ctgcatagcc gcatttgcga cacggtgtta atagtgctgg cgctgctgct
    76141 gttgctgcca ccgttgctgg cggtgatcgt cgatggggta aatcgccagt tgccggaagt
    76201 gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc
    76261 aggtgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg aactgcgggc
    76321 gcggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga tcctcgccat
    76381 gccggggatt gtgctggcta ccggcttctt tttactgctc aacaacacta tcggcctgcc
    76441 acaatctgct gacggcattg tgattttcac caatgcgtta atggcgatcc cttatgcgct
    76501 gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagtatgt tgtgtcagtc
    76561 gctgggcatt gaaggctggt cgcgcttaaa agtggtcgag ctgcgcgccc tgaaacgtcc
    76621 actggcgcag gcgctggctt ttgcctgcgt gctgtcgatt ggtgattttg gcgtagtggc
    76681 gttgttcggt aacgatgatt tccgtaccct gccgttttat ctctaccagc aaattggctc
    76741 ctatcgcagc caggacggcg cggtcaccgc gttaattctg ctactactct gttttctgct
    76801 gtttaccgtg attgaaaaac taccggggcg aaatgttaaa actgactgat atcacctggc
    76861 tttatcacca tttgccgatg cgttttagct taacggtaga acgcggcgag caggtggcaa
    76921 tcctcgggcc aagcggcgcg ggtaaaagta ccctgctgaa tttgattgcc ggttttctga
    76981 cgccagccag cggttcgctg actatcgatg gcgttgatca caccacgacg ccgccgtcac
    77041 gccgtccggt gtcgatgctg tttcaggaga acaacctgtt cagccacctg acggtcgcgc
    77101 agaacatcgg gctggggcta aatccggggt tgaaactgaa cgcggcacag caggagaaaa
    77161 tgcacgcgat tgcccgtcag atggggattg ataatttaat ggcgcggtta ccgggcgagc
    77221 tttccggcgg tcagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc gaacagccga
    77281 ttttattgct cgatgaaccg ttctctgcgc tcgatccggc gttacgtcag gagatgctga
    77341 cgctggtgag cacgagctgc cagcagcaaa aaatgacgct attgatggtg tcgcacagtg
    77401 tggaagatgc ggcgcggatc gccacacgct cggtggtggt cgccgacggg cgcatcgcct
    77461 ggcagggtaa gactgaagaa ttgttgagcg gtaaggcgag tgcttcggca atattgggga
    77521 ttacgggtta gtatgcactg tcagatgcgg cgtgaacacc ttatccgacc tacggacagt
    77581 gcgttttgta ggcctgataa gacgcggtca gcgtcgcatg aggcaacccg cacaagacaa
    77641 tcccctaaac cccaaccact ttacgcaaaa tatcgatata caccggcatc aacgggtggc
    77701 gaattaacac caccagcgcc accacgccga tggcagaaat caacggcgtc agccacaaca
    77761 aacggccgcg cgacaaataa tgacttaaac gatcggttgc tttaccgctg cgccataacc
    77821 gccagcacag ccagccacca acccacaaaa ataccgccgt cgccagcagc aaccatttaa
    77881 actcgccgct ctgcattccg gctggaatat cgatcgccgc gcccgccaga atcccaggca
    77941 ggaagtaaaa cggtggccac agcaggcagc cgataatatt cggcgtaatg aattttgcca
    78001 ccggcagatc cagcattccc gccaccattg gcaccagcgg acgcgtcggg ccaacaaagc
    78061 gaccgaccag aatggtgaac atgctgtgtt gatgcaacgc atgttcagtt ttatcgagta
    78121 atgctttgtt tttcttcaga aatgaccagc gatgcaacgg ctttttaaaa cgccagccca
    78181 gccagaagga gatccagtcg cccagcagac aaccgacaat ccctgccagc caggcgtgcc
    78241 agaaacttaa ctcgccgctg ccaatcagcg ctcccagccc cgccatcagc accgtaccgg
    78301 gtagaatcaa accgaccagc gccagcgact ccagaaaggc caccaacacc accgccatca
    78361 atgaatacac ggtagattgg gtaataaagt gttccagcaa tgcttgcata atgtgcctgt
    78421 caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc
    78481 tgattcgtta ctaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg
    78541 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
    78601 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
    78661 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
    78721 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
    78781 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtaca
    78841 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
    78901 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
    78961 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaaa
    79021 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
    79081 aagtaaaccc actggtgata ccattcgcga gcctccggat gacaaccgta gtgatgaatc
    79141 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaga caaattctcg tccctgattt
    79201 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
    79261 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
    79321 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
    79381 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
    79441 tgcgtctttt actggctctt ctcgctaacc caaccggtaa ccccgcttat taaaagcatt
    79501 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
    79561 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
    79621 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
    79681 ccatacccgt ttttttggat ggagtgaaac gatggcgatt gcaattggcc tcgattttgg
    79741 cagtgattct gtgcgagctt tggcggtgga ctgcgccagc ggtgaagaga tcgccaccag
    79801 cgtagagtgg tatccccgtt ggcaaaaagg gcaattttgt gatgccccga ataaccagtt
    79861 ccgtcatcat ccgcgtgact acattgagtc aatggaagcg gcactgaaaa ccgtgcttgc
    79921 agagcttagc gtcgaacagc gcgcagctgt ggtcgggatt ggcgttgaca gtaccggctc
    79981 gacgcccgca ccgattgatg ccgacggtaa cgtgctggcg ctgcgcccgg agtttgccga
    80041 aaacccgaac gcgatgttcg tattgtggaa agaccacact gcggttgaag aagcggaaga
    80101 gattacccgt ttgtgccacg cgccgggcaa cgttgactac tcccgctata ttggcggtat
    80161 ttattccagc gaatggttct gggcaaaaat cctgcatgtg actcgccagg acagcgccgt
    80221 ggcgcaatct gccgcatcgt ggattgagct gtgcgactgg gtgccagctc tgctttccgg
    80281 taccacccgc ccgcaggata ttcgtcgcgg acgttgcagc gccgggcata aatctctgtg
    80341 gcacgaaagc tggggcggct tgccgccagc cagtttcttt gatgagctgg acccgatcct
    80401 caatcgccat ttgccttccc cgctgttcac tgacacctgg actgccgata ttccggtggg
    80461 caccttatgc ccggaatggg cgcagcgtct cggcctgcct gaaagcgtgg tgatttccgg
    80521 cggcgcgttt gactgccata tgggcgcagt tggcgcaggc gcacagccta acgcactggt
    80581 aaaagttatc ggtacttcca cctgcgacat tctgattgcc gacaaacaga gcgttggcga
    80641 gcgggcagtt aaaggtattt gcggtcaggt tgatggcagc gtggtgcctg gatttatcgg
    80701 tctggaagca ggccaatcgg cgtttggtga tatctacgcc tggtttggtc gcgtactcgg
    80761 ctggccgctg gaacagcttg ccgcccagca tccggaactg aaagcgcaaa tcaacgccag
    80821 ccagaaacaa ctgcttccgg cgctgaccga agcatgggcc aaaaatccgt ctctggatca
    80881 cctgccggtg gtgctcgact ggtttaacgg tcgtcgcacg ccaaacgcta accaacgcct
    80941 gaaaggggtg attaccgatc ttaacctcgc taccgacgct ccgctgctgt tcggcggttt
    81001 gattgctgcc accgcctttg gcgcacgcgc aatcatggag tgctttaccg atcaggggat
    81061 cgccgtcaat aacgtgatgg cgctgggcgg catcgcgcgg aaaaaccagg tcattatgca
    81121 ggcctgctgc gacgtgctga atcgcccgct gcaaattgtt gcctctgacc agtgctgtgc
    81181 gctcggtgcg gcgatttttg ctgccgtcgc cgcgaaagtg cacgcagaca tcccatcagc
    81241 ccagcaaaaa atggccagtg cggtagagaa aaccctgcaa ccgcgcagcg aacaggcaca
    81301 acgctttgaa cagctttatc gccgctatca gcaatgggcg atgagcgccg aacaacacta
    81361 tcttccaact tccgccccgg cacaggctgc ccaggccgtt gcgactctat aaggacacga
    81421 taatgacgat ttttgataat tatgaagtgt ggtttgtcat tggcagccag catctgtatg
    81481 gcccggaaac cctgcgtcag gtcacccaac atgccgagca cgttgttaat gcgctgaata
    81541 cggaagcgaa actgccctgc aaactggtgt tgaaaccgct gggcaccacg ccggatgaaa
    81601 tcaccgctat ttgccgcgac gcgaattacg acgatcgttg cgctggtctg gtggtgtggc
    81661 tgcacacctt ctccccggcc aaaatgtgga tcaacggcct gaccatgctc aacaaaccgt
    81721 tgctgcaatt ccacacccag ttcaacgcgg cgctgccgtg ggacagtatc gatatggact
    81781 ttatgaacct gaaccagact gcacatggcg gtcgcgagtt cggcttcatt ggcgcgcgta
    81841 tgcgtcagca acatgccgtc gttaccggtc actggcagga taaacaagcc catgagcgta
    81901 tcggctcctg gatgcgtcag gcggtctcta aacaggatac ccgtcatctg aaagtctgcc
    81961 gatttggcga taacatgcgt gaagtggcgg tcaccgatgg cgataaagtt gccgcacaga
    82021 tcaagttcgg tttctccgtc aatacctggg cggttggcga tctggtgcag gtggtgaact
    82081 ccatcagcga cggcgatgtt aacgcgctgg tcgatgagta cgaaagctgc tacaccatga
    82141 cgcctgccac acaaatccac ggcgaaaaac gacagaacgt gctggaagcg gcgcgtattg
    82201 agctggggat gaagcgtttc ctggaacaag gtgacttcca cgcgttcacc accaccttta
    82261 aagatttgca cggtctgaaa cagcttccag gtctgcccgt acagcgtctg atgcagcagg
    82321 gttacggctt tgcgggcgaa ggcgactgga aaaccgccgc cctgcttcgc atcatgaagg
    82381 tgatgtcaac cggtctgcag ggcggcacct cctttatgga ggactacacc tatcacttcg
    82441 agaaaggtaa tgacctggtg ttcggctccc atatgctgga agtctgcccg tcgattgccg
    82501 tagaagagaa accgatcctc gacgttcagc atctcggtat tggtggtaag gacgatcctg
    82561 cccgactgat cttcaatacc caaaccggtc cagcgattgt cgccagcctg attgatctcg
    82621 gcgatcgtta ccgtctgctg gttaactgta tcgacacggt gaaaacaccg cactccctgc
    82681 cgaaactgcc ggtggcgaat gcactgtgga aagcgcaacc ggatctgcca actgcttccg
    82741 aagcgtggat cctcgctggt ggcgcgcacc ataccgtctt cagccatgcg ctgaacctca
    82801 acgatatgcg ccagttcgcc gagatgcacg acattgaaat cacggtgatt gataacgaca
    82861 cccgcctgcc agcgtttaaa gacgcactgc gctggaacga agtgtattac ggatttcgtc
    82921 gctaagtagc cacatcaggt atgtaacgcc tgatgcgacg ctgacgcgtc ttatctggcc
    82981 tacacgctgc gattttgtag gccggataag caaagcgcat ccggcattca acgcctgatg
    83041 cgacgctggc gcgtcttatc aggcctacgc gctgcgattt tgtagaccgg ataagcgaag
    83101 cgcatccggc acgaaggagt caacatgtta gaagatctca aacgccaggt attagaagcc
    83161 aacctggcgc tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt
    83221 gatcgcgagc gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc
    83281 gctgacgata tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag
    83341 ccctcctccg acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc
    83401 attgtgcata cgcactcgcg ccacgccacc atctgggcgc aggcgggtca gtcgattcca
    83461 gcaaccggca ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg
    83521 accgacgcag aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc
    83581 tttgaaaaac agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc
    83641 ccgtttgcat ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag
    83701 gtcgcttata tggggatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa
    83761 acgctgctgg ataaacacta tctgcgtaag catggcgcga aggcatatta tgggcagtaa
    83821 tgactgtata aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt
    83881 gatggatttt cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc
    83941 cgcaagggac agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc
    84001 ttgcaccgca agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata
    84061 ttttgcaggg tgaacaaggc tttcgtctga caccgctggc gttaaaggat tttcaccgcc
    84121 agccggtgta tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc
    84181 tgcgtgaagg tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga
    84241 tggagcggtt tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg
    84301 ttaatgcccg tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag
    84361 atattgaaac cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc
    84421 gcatcgttta tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg
    84481 aatacgtcgc cagccgcccg ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg
    84541 atcccgatgt gatcatcggc tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac
    84601 atgccgagcg ttaccgtctt ccgctgcgtc ttgggcgcga taataacgag ctggagtggc
    84661 gcgagcacgg ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg
    84721 acggtatcga ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg
    84781 tcgctcagga gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg
    84841 aaattgaccg ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt
    84901 gcgagctggt gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag
    84961 caacggtgaa cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc
    85021 tctattttcc gcgaatgcat cgcgctggtt atgtcgcgcc taatctcggc gaagtgccgc
    85081 cgcacgccag ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc
    85141 tggtgctgga ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg
    85201 tcgggctggt tgaaggcatg gcgcaacctg atccagagca cagtaccgaa ggttttctcg
    85261 atgcctggtt ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg
    85321 ggcgcgatga agccaaacgt cagggtaata agccgctttc gcaggcgctg aagatcatta
    85381 tgaatgcctt ttatggcgtg ctcggcacca ccgcctgccg cttcttcgat ccgcgcctgg
    85441 catcgtcgat caccatgcgt ggtcatcaga tcatgcggca gaccaaagca ttgattgaag
    85501 cgcagggcta cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg
    85561 cacattcgga agaagaggcg gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct
    85621 ggtgggcgga aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa
    85681 ctcatttctg ccgttttctt atgccaacca ttcgcggagc tgataccggc agtaaaaagc
    85741 gctacgccgg gctgattcag aagggcgata agcagcggat ggtgtttaaa gggctggaaa
    85801 ccgtgcgcac cgactggacg ccgctggccc agcagtttca gcaggagctg tacctgcgta
    85861 tcttccgcaa cgagccatat caggagtacg tccgccaaac catcgacaaa ctgatggcgg
    85921 gtgaactgga tgcgcggctg gtttaccgta aacgccttcg ccgtccgctg agcgaatatc
    85981 agcgtaatgt tccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aatcaaaagc
    86041 gcggtcgccc cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg
    86101 gcccggagcc gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc
    86161 agctacaacc cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta
    86221 tgaccgggca acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat
    86281 gcgtgacgaa tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata
    86341 acaccactac ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag
    86401 ccgaacacat atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt
    86461 gggacttgga accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac
    86521 tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc
    86581 tggtgatacc attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga
    86641 aaatggcttg ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct
    86701 gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc
    86761 cgggcagatt gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag
    86821 cgaacagttc cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgatcccgca
    86881 tcagctcaac atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga
    86941 cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc
    87001 tggcgctgct gaacgtgtgc tgattatcgt cccggaaacc ttacagcatc agtggctggt
    87061 agaaatgctg cgccgtttca acctgcgctt tgctctgttt gatgatgagc gttatgccga
    87121 agctcagcac gatgcttaca acccgtttga caccgaacag ctggtgattt gctcgctgga
    87181 ttttgcccgt cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct
    87241 ggtggtcgat gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca
    87301 ggccattgaa caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga
    87361 acagctgggg atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca
    87421 cgattttgcg cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc
    87481 catgctgctg gcaggtaaca aactgagcaa tgacgaactg aacatgctcg gcgagatgat
    87541 cggcgagcag gatatcgagc cgctgttgca ggcagcaaac agcgacagcg aagatgccca
    87601 gagcgcccgt caggagctgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct
    87661 gttccgtaac acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa
    87721 gctgccgcta ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg
    87781 taaaagtgcg gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt
    87841 tgaaggtgat aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta
    87901 cctgaccagc catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct
    87961 gcaactggag caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg
    88021 tatgtcgatt atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc
    88081 acaggtactg ctgtgctcag aaatcggttc tgaaggacgt aactttcagt tcgccagcca
    88141 catggtgatg tttgacctgc cattcaaccc ggatctactg gagcagcgta ttggtcgtct
    88201 ggatcgtatc ggccaggcgc acgatattca gatccatgtg ccttatctgg agaaaaccgc
    88261 tcagtcggtg ctggtgcgct ggtatcacga aggtctggat gcatttgagc acacctgccc
    88321 gaccggacgc actatttacg atagcgtata caacgatctg attaactatc tggcttcacc
    88381 ggatcaaacc gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct
    88441 gaaagcacag ctggaacagg gtcgtgaccg cctgctggaa atccactcca acggtggcga
    88501 aaaagcccag gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc
    88561 cttcgccatg aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat
    88621 gatcgtgctg acgccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga
    88681 tggcatcacc atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg cacagtttat
    88741 tacctgggag catccgctga tccgcaacgg tctggatctg atcctttctg gcgataccgg
    88801 tagcagcacg atttcactgt taaaaaacaa agcgttgcca gtaggtacgc tgttggtgga
    88861 actgatttat gtggttgaag cccaggctcc gaagcagttg cagctcaacc gcttcctgcc
    88921 accgacgccg gtacgtatgc tgctggataa aaacggcaac aacctggcgg cgcaggtaga
    88981 gtttgaaacc tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt
    89041 taacgccgtg cagcaggatg ttcacgctat ccttcaactg ggtgaagcgc agatcgagaa
    89101 atctgcccgt gcattgattg atgcagcgcg taacgaagcc gacgaaaaac tgtctgccga
    89161 gctgtctcgt ctggaagctc tgcgtgcagt gaacccgaac attcgtgacg acgaactgac
    89221 cgccattgag agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gttggcgtct
    89281 ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg agtcgaaaat ggggatggaa
    89341 aactacaatc caccgcagga accctggttg gttatcctgt atcaggatga ccatattatg
    89401 gtggtcaaca agccgagcgg tttgttgtca gtgccgggtc gtctggaaga gcacaaagac
    89461 agcgtgatga cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat
    89521 atggctacca gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa
    89581 cgccagttcc gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca
    89641 tcccccgcag aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa
    89701 cagaaagtct gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat
    89761 gcggcggata acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg
    89821 cgtgtgcata tgctggcgct ggggcatccg attctcggcg atcgttttta tgcatcacca
    89881 gaagcgagag cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat
    89941 ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg
    90001 cgcagttcgg taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc
    90061 tgatgcgacg cgggccgcgt cttatcaggc ctacagttta aaacttattt aaaccctttc
    90121 tgctgcttta tcagctcata tgcctgctga atttcctgcg ctttctgctt cgccatctcc
    90181 atcatctcag gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc
    90241 ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta
    90301 caggcatctt ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc gccagattgc
    90361 tgctgataac cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac
    90421 tgggcgcggg agatccctaa ttcttcagca atgacataca gcaccgcccg ttcattcggg
    90481 tgcagtgaac catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag
    90541 tcaaaacgac caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaatta
    90601 tctgattttc ccacccggaa cgcgttttgc gccgcagtac gggaagcgcc atgaaggttc
    90661 attcggtcca tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat
    90721 ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc
    90781 tggttggcga accacgccat tttacggcta cgggctttat caaacatatg gccaattaac
    90841 aggcctaaca ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg
    90901 atttttcccc aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc
    90961 cacaatataa aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat
    91021 aatttgcatt atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga
    91081 caggattaac actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc
    91141 aacgttaccg atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct
    91201 ggccaccatg attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc
    91261 acagtgcatg ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga
    91321 cttacccgtg actatcaatg ctgaccacgc gaaaggggac tacccggatg acgccgtgtt
    91381 tactggcagc gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct
    91441 ccatcaaaaa gaggcactag gacaaccgga gccggtacgt accgttgatg cgctcggtaa
    91501 tgtccattac gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa
    91561 caccaaagat accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg
    91621 taaagcggac ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag
    91681 ctttacctcc tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca
    91741 tgaccgcgaa gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtacc
    91801 gatcttttat agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt
    91861 gatcccgaac gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg
    91921 gaacatcgcg ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa
    91981 catcatgtgg gagaacgaat tccgctacct ctcccaggct ggcgctggct tgatggaact
    92041 ggactatctg ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg
    92101 tcgttggtta ttctactgga accactccgg ggtcatggat caggtgtggc gtttcaacgt
    92161 cgactacacc aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc
    92221 cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa
    92281 tgccaccgtt tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc
    92341 ggcagagccg cagttagacg ttaattacta ccagaatgat gttggtccgt ttgatacgcg
    92401 tatttacggc caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg
    92461 tgttcacctg gaaccgacca tcaatttgcc tctctctaat aactggggca gcatcaatac
    92521 cgaagcgaag tttctggcaa cccattatca gcaaaccaat cttgactggt ataactccag
    92581 aaacacgacc aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg
    92641 caaaatggtc tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga
    92701 accgcgcgcg cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga
    92761 ctcgtctctg ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct
    92821 tgaccgtatt gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga
    92881 tgctgccgtt gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg
    92941 cactggcgat gacaacataa catgggagaa tgacgacaaa acgggctcac tggtgtgggc
    93001 aggcgacacc tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga
    93061 tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga
    93121 ccgtctggta cagctgaatt accattacgc cagcccggaa tatattcagg ctacgctgcc
    93181 taagtactat tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc
    93241 cagctggcca attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc
    93301 gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg
    93361 cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga
    93421 caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac
    93481 gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt
    93541 accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc
    93601 tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata
    93661 aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc
    93721 agtcggtaaa actgaacgct gcgcaggcaa ggcagcagct tcctgatgac acgacgctgc
    93781 gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa
    93841 tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga
    93901 acaacatgac gctggatcag atgcgcggcc gtctggctta cgatggtctg aactacaaca
    93961 cttatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc
    94021 gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc
    94081 aaaacgacgc cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc
    94141 cgacctctga tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc
    94201 gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga
    94261 acggcggcca gatggggtgg ggccgtattc aggagttgcc ggggatcttc gcccaggcat
    94321 taagcaccgc gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata
    94381 ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg
    94441 ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc cgcgtgaaac
    94501 tggagcaaat tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag
    94561 agttctcgca ggatccaggt tctgctaacc agggcggtga tctcggctgg gctacaccag
    94621 atattttcga tccagccttc cgtgacgccc tgacccgcct gaacaaaggt caaatgagtg
    94681 caccggttca ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg
    94741 ataaaaccga cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct
    94801 cggaagaagc agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga
    94861 gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg
    94921 cccggactta gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc
    94981 cgatgccact ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc
    95041 ttattccccc aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt
    95101 cgcgctacgt gaatctgtca ctgcggggca gttagcgatt gaaaatgggc attacgtggt
    95161 ggagacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac
    95221 aggccccgtg cataaaggcg tcattaacga cgcaagcatt ccgtttaccg gtcataccga
    95281 gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact
    95341 tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc
    95401 tgcacttttg cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa aatttggtat
    95461 tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat
    95521 gggtacggaa gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat
    95581 gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc
    95641 cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt
    95701 cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg
    95761 caccgcgctt gaactggcgg gacgtggcga agccgatgtc ggcagtttta ttacggcgct
    95821 taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac
    95881 ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc acttcgtgat cgacagcatt
    95941 gtttctgcca ttaacccgca aaagggccag gcaatggtcg aaatcggccc cggtctggcg
    96001 gcattgaccg agccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc
    96061 gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag
    96121 caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt
    96181 gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat
    96241 actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt
    96301 gcaggaccga acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac
    96361 gtgatcccgg tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc
    96421 gccgtcgtgc gcctagtccc tcatgcaacg atgcctcacc cggttaaaga tgttcgcgtg
    96481 ttgagccgca tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc
    96541 ggcaacctgt ttagcgtcga ggtgttaacc ggaatgggga tcgacccggc gatgcgagcg
    96601 gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct
    96661 ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct
    96721 acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca
    96781 tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg
    96841 gcaatggccg tgaaaccgaa gtccatggcg aaggagtggt tggcgtccag ccacttatcg
    96901 cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca
    96961 tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg
    97021 tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg
    97081 gcgacgttca tggttgttac gatgaactga tcgcattgct gcataaagta gaatttaccc
    97141 ccgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcggcccg ggttcgctgg
    97201 atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg
    97261 atctgcatct gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga
    97321 caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctc
    97381 tgctgcaaat cgacgaagag aaaaagctgg tgatggccca cgcagggatc acgccgcagt
    97441 gggatctgca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg tcgagtgact
    97501 cctatccctt ctttcttgac gctatgtacg gcgatatgcc aaataactgg tcaccggaat
    97561 tgcgggggct gggaagactg cgttttatca ccaacgcctt tacccgtatg cgtttttgct
    97621 tcccgaacgg tcaactggat atgtacagca aagaatcgcc ggaagaagcc cctgccccac
    97681 tgaaaccgtg gtttgcgatt cctggccctg tcgctgaaga atacagcatc gcctttggtc
    97741 actgggcatc gctggaaggc aaaggtacgc cggaaggtat ttacgcgctg gataccggct
    97801 gctgctgggg tgggtcatta acctgcctgc gctgggaaga taaacagtat tttgtccagc
    97861 cgtcgaaccg gcataaggat ttgggcgaag cggcggcgtc ttaaacacag cctgatacag
    97921 gaaggccgga taagacgcga ccggcgtcgc atccggcact agccgtaaat tctatacaaa
    97981 cttaccgccg ctccagaatc tcaaagcaat agctgtgaga gttctgcgca tcggcgtcgt
    98041 ggaattcgct gaataccgat tcccagtcat ccggctcgta atccgggaaa tgggtgtcgc
    98101 cttccacttc tgcgtcgata tgcgtcagat acagtttttg cgcttttggc aggaactgct
    98161 cataaacgcg accgccgcca atcaccatga tttctggtac gtcaccacac gccgcaatgg
    98221 cttcatccac cgatttcacc cacgttacgc gatcgtccgt acccggttga ctgctgagga
    98281 taatattttt gcgtcctggc aacggacgac cgattgattc ccaagtatgg cggcccataa
    98341 tcacgggttt atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccacg
    98401 gcatggcgtt ttccatgccg ataacgcgat ctaccgctaa cgccgcaatc agactgatca
    98461 ttgagatttc ccgataaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac
    98521 tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctcccc
    98581 ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttac
    98641 gtcactcttt attaagatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt
    98701 ttaccttctt ctgttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtcc
    98761 tcggtgatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg
    98821 gtgtcattct caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca
    98881 tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagcgc cagacgcccg
    98941 gttttcagcg caccttcaaa ggtttcacgc tccggctttt caaggcctgc ctgacgcaaa
    99001 cgaatgtagt gatcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct
    99061 ttcaccatct ccgtcagttg caggttggtt tgcggatcgt caatagcgtt aatcagcact
    99121 tccgctttcg ccgctccggc agattccagt aaatccatcc gcgtggcatc gccataaaac
    99181 actttcatac caaatttacg cagcgtttcg atatggtccg gatcgtgatc gagcaccacc
    99241 attttcaccc cgctggagag cagtaaacgt ccggtaatct gtccaaaacg accgaatccg
    99301 gcgataatca cgcgcggctg ttcttcatcg atctcatcgg cttcacgcgc ctcctccgta
    99361 gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc catcgacagc
    99421 gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc
    99481 gcaccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccagcga
    99541 cgctgtttat ttggcacctg caacggtcgg gcaatcagcc acagcatggc gattttgatg
    99601 atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaatcag cgtgccaaag
    99661 tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaacggttcg
    99721 atatcgcttt ccaacgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac
    99781 gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacaagg
    99841 aataacgcca cgacactaaa cacttcccgc aagccagagc gggcaacaaa acgcagcgcc
    99901 ggacgcgtaa catagcgccc cagcaatacc accagtacca gcgcacccgc cacttttaac
    99961 gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgccgccag tagcgggatc
   100021 atcgccacca gcgggattgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcgg
   100081 cacatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgttgat
   100141 gagagcgcca gcgtcatacc gatcaattcc gcgacctgcc agcgcagccc aagtaacata
   100201 cagaacagcc ccagcaggcc accgcaaatc accatctgca atgcgccacc gccgaacact
   100261 gccgcacgca gcttccacag cctttgtgga tcgagttcga ggcgataata aacagcatca
   100321 gcaccacccc aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc
   100381 cccacgggcc aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac
   100441 gtaccgcaat gggtacaatc agcgccgccg atccgaggta gatcagcgcc tgaaccagcg
   100501 tatgactatc catgatgggc ctcctgccat tccagcagac gttgcttata gtggcgcgcc
   100561 tgcccttcga gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc
   100621 cagttcagcc cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc
   100681 ggatgcgcac caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa
   100741 tgtttgccat gcagcgccgt gccgccgtga ccgtaagccc agccgtgcga caaaacttta
   100801 tcgatccaaa gtttaagaag cggaggaatg ctgtaccact gcatcgggtg ctgccagacg
   100861 atcagatcgg cgcgagagag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga
   100921 tagagttgat aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc
   100981 cgtttattcg catgggaatg atgcggatac ggatgcgcat aaatgatgag gatcatagtt
   101041 attagcccgc gtgtttacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc
   101101 ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaaataat cccattctct
   101161 gcccgcggat cgtcatattg ctggtctgcg ccagtgacat tcctcgagtc tccggggcaa
   101221 acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac
   101281 tgataccgta attattgata aatatcggca gtgcccaggt cgaaacaatg gtgccaatac
   101341 gacttaagga cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat
   101401 taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg
   101461 ccatcactac cagccagatc cccatatccg ggattagccc caaaaccgcc agcgccagcg
   101521 tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca
   101581 tcggcggaat acagccgagc ataaagaaca ggctaatcac cacattccct agtgccgcgt
   101641 ttttgccaac ccccaatccc aacgaaccaa cgatttgcgg gccaaaggtg taaatggcga
   101701 acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc
   101761 ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg
   101821 gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct ttgcgtaata
   101881 accagcgggg agattctggc agttcgaatc gaccaatcaa aatcaacaaa caagggatcg
   101941 ccgcgctacc cagcatccag cgccaaccac cttccacatc ataaagccag tagccgacca
   102001 gatcggcgca ggtcgcgccg acataccaca tcgcggcaat aaagctgatg gaaaacgccc
   102061 gctgacgggt actggagaac tcggtgatca tcgaagtggc gatgggataa tctgcaccga
   102121 tgacaatgcc gatgagtacc cgcatcacca acagttcgac gggggatgaa acacacatcg
   102181 tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga
   102241 ctttatcgga gatatagcca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg
   102301 cgcccagcaa gccaatccag tcagcgtcca gtttcagcgc cggagtcagt tgctccagcg
   102361 ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt ccccacagca
   102421 aaatgcggcg gtgaatagag gagaatttga gatcgtcaaa gtttctggac ggttgcatgg
   102481 gttattcctg tttttttgca atgagagtca gtacggcgtt acgtatcaaa ccaacatcag
   102541 ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc cagcgccgac
   102601 cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc gaagcgcaca
   102661 ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag cagctccagc
   102721 acctgtttat cagcatcggc cttcacaaca atgtgcggat gctcttcatc gacattgaat
   102781 ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg gtcactcctt
   102841 tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg aggattttct
   102901 tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc acgcccaccg
   102961 ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaacgccggt agtttctggt
   103021 acatgcgcat atcgcgcagc gggccactct ccagatgctg acgatattcc gcgagttttt
   103081 gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca gcttccccgg
   103141 cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac attccggcgg
   103201 catcaccagc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg atgcctgctt
   103261 ccggcactac gtgagcggaa tattccacca gcttgccgcc cgcgatcagc ggtgcaacgg
   103321 ccggatgatg tttgaaatct tccagcattt gcggcaccga ttttttcgcg tcatgcagat
   103381 gatgcagacc acaaaccagc cccagcgaca gggtattttc actggtataa aggaagccgc
   103441 cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc tgattaccct
   103501 gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc ttcacgccaa
   103561 ccgccacatc cgtcggttta acgcgttttg ccatccccaa cttttcggca aggatggagt
   103621 tcaccccatc agcaaggatc accgttttcg cttcaatcac atcgccatcg gcttctacac
   103681 cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta attaactgcg
   103741 cgctcgcttc ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc aaaacggagt
   103801 aagaacgctg ggatggcgag gtttcgtcac cgttgcagta gtccatagtc atcgctgact
   103861 tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg gcggaatcgg
   103921 cgaaacctgg aataatgtgt tccagactgt gggcatagag acgcccgcca gtaacgttct
   103981 tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgccccttcg cgggcgagca
   104041 ccagtgcggc aaccgaaccg gcaagccctg caccgacgat gatggcgtca aagatatctt
   104101 cggacataag agctccctgt cagcggcaga gccgtaatgc agccctgcca gagtggatca
   104161 acgcgctaaa gctgcggtca gtgccggaag gatcttcacg gcatcgccaa caatgccgta
   104221 atccgcgtac tggaagatcg gcgcattttt atctttattg atggcgaaaa tggtttgcga
   104281 cgcgttagcg ccaaccatgt gctggatctg cccggagatc cccaccgcca ggtacagttc
   104341 aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc atttttcgtt
   104401 ttctgccacc ggacgagaac aggccaactc cgcacctatc gccttgcaaa gctgttctgc
   104461 cagcgcaatg ttctctttgc tgccaatgcc gcgaccgacg ctgaccacca gacgggcttt
   104521 gtcgagatcg acgctgttgc tctggcgcgc ctggctgagc cgcatcgaac gtgccgctgc
   104581 tgatggtcag taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccgccgt
   104641 aaaccatgtg tttcactgtt gctttaccgt cctgtacgct gacggtgctg gcatcgttag
   104701 acaccgccgc tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt
   104761 ttggcaacag caccagaccg tcagcgccgt gctggcgaat agtgtcagcc atgacaccgg
   104821 cgtaatcttc gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc
   104881 cgagctggat tgcctgtgcg ccgtcggcat cattgaggac aaaggcattg atttgattag
   104941 ctaaagcctg cgcaccgttc atcagttccg gcagacgaga gggggtatcg ctgaataccc
   105001 agacttgaga aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc
   105061 agcaaatgcg gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgctcgcg
   105121 ctgtttcggc gcggcaacct gttgttcaga ccaggcgtcc acggcgttaa aaccaatatc
   105181 cgccgccgac catacctgga ctggcttttt cgccgcgccg agaatggctt tcatcgaagg
   105241 aatttgtggg gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa
   105301 ggtttcggtt tcatcttcca gttcgcgctc aacggtgagg gtatctgccg tcagggagat
   105361 aattttgctg acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac
   105421 ctgctgggca taaaggtcgg aagaaccatc gccacagagg atcagatcaa agcctgcttt
   105481 ctgggcggct gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc
   105541 atcaatcacc acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc
   105601 gttggtcagg gctttaccgc ccacacttaa ggctgtcacc tgcgcctctg ctgcctgttg
   105661 ctttagctgg caagccgctt caatagcgtt gagatcgtat tggcttattt tggcatcggc
   105721 tttgctgaag tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac
   105781 gcacttatag caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt
   105841 catatcggtg gtgttaatta cagatatcac gccctttaat ttggaaataa aactttaatc
   105901 accaatattg aaaatgtcac acgcagataa atccaacttt caatattgtt aagttcctca
   105961 ccaatattga aaacatggcg tagcaaaaag aaattttcaa tattgtttta tagatcacca
   106021 atattgaaag tcagatgcta tttttttacc cacaaaagaa cataacaaac tgattaataa
   106081 ccaaaattaa cggaaaacgt cctctattca cccgaaacaa aaatgtgaca acaatcacag
   106141 aatacagctt attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa
   106201 taattatatt aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa atattcgttt
   106261 tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt
   106321 ttctttccgc cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat
   106381 gcggcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatggg
//
LOCUS       DADKXM010000002        77854 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.002, whole genome shotgun sequence.
ACCESSION   DADKXM010000002 DADKXM010000000
VERSION     DADKXM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.002"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..2265
                     /locus_tag="JQR05_000094"
                     /pseudo
     CDS             1..2265
                     /locus_tag="JQR05_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2266..3915
                     /locus_tag="JQR05_000095"
     CDS             2266..3915
                     /locus_tag="JQR05_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY5322360.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIHQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELVYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3920..4696
                     /locus_tag="JQR05_000096"
     CDS             3920..4696
                     /locus_tag="JQR05_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY5322361.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4971..7820
                     /gene="rcsC"
                     /locus_tag="JQR05_000097"
     CDS             4971..7820
                     /gene="rcsC"
                     /locus_tag="JQR05_000097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY5322362.1"
                     /translation="MKYLASFRTTLKASHYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JQR05_000098"
     CDS             complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JQR05_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY5322363.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JQR05_000099"
     CDS             complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JQR05_000099"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY5322364.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTLRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESDVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12098..13219
                     /gene="ompC"
                     /locus_tag="JQR05_000100"
     CDS             12098..13219
                     /gene="ompC"
                     /locus_tag="JQR05_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY5322365.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13331..14386
                     /gene="apbE"
                     /locus_tag="JQR05_000101"
     CDS             13331..14386
                     /gene="apbE"
                     /locus_tag="JQR05_000101"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY5322366.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14460..15524
                     /gene="ada"
                     /locus_tag="JQR05_000102"
     CDS             14460..15524
                     /gene="ada"
                     /locus_tag="JQR05_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY5322367.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15524..16174
                     /gene="alkB"
                     /locus_tag="JQR05_000103"
     CDS             15524..16174
                     /gene="alkB"
                     /locus_tag="JQR05_000103"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY5322368.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16250..17893
                     /gene="yojI"
                     /locus_tag="JQR05_000104"
     CDS             16250..17893
                     /gene="yojI"
                     /locus_tag="JQR05_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY5322369.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18111..19757
                     /gene="mqo"
                     /locus_tag="JQR05_000105"
     CDS             18111..19757
                     /gene="mqo"
                     /locus_tag="JQR05_000105"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY5322370.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGGAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNRHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(19907..20395)
                     /gene="eco"
                     /locus_tag="JQR05_000106"
     CDS             complement(19907..20395)
                     /gene="eco"
                     /locus_tag="JQR05_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY5322371.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20480..20695)
                     /locus_tag="JQR05_000107"
     CDS             complement(20480..20695)
                     /locus_tag="JQR05_000107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322372.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAIIFTRD
                     ISAGLLPHQNLTLKEQVKLSECSLSYS"
     gene            20804..21298
                     /gene="napF"
                     /locus_tag="JQR05_000108"
     CDS             20804..21298
                     /gene="napF"
                     /locus_tag="JQR05_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY5322373.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21288..21551
                     /gene="napD"
                     /locus_tag="JQR05_000109"
     CDS             21288..21551
                     /gene="napD"
                     /locus_tag="JQR05_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY5322374.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQQNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21548..24034
                     /gene="napA"
                     /locus_tag="JQR05_000110"
     CDS             21548..24034
                     /gene="napA"
                     /locus_tag="JQR05_000110"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY5322375.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            24041..24736
                     /gene="napG"
                     /locus_tag="JQR05_000111"
     CDS             24041..24736
                     /gene="napG"
                     /locus_tag="JQR05_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY5322376.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24723..25586
                     /gene="napH"
                     /locus_tag="JQR05_000112"
     CDS             24723..25586
                     /gene="napH"
                     /locus_tag="JQR05_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY5322377.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25583..26032
                     /gene="napB"
                     /locus_tag="JQR05_000113"
     CDS             25583..26032
                     /gene="napB"
                     /locus_tag="JQR05_000113"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY5322378.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            26042..26644
                     /gene="napC"
                     /locus_tag="JQR05_000114"
     CDS             26042..26644
                     /gene="napC"
                     /locus_tag="JQR05_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY5322379.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26663..27280
                     /gene="ccmA"
                     /locus_tag="JQR05_000115"
     CDS             26663..27280
                     /gene="ccmA"
                     /locus_tag="JQR05_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY5322380.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27277..27939
                     /gene="ccmB"
                     /locus_tag="JQR05_000116"
     CDS             27277..27939
                     /gene="ccmB"
                     /locus_tag="JQR05_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY5322381.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27981..28718
                     /gene="ccmC"
                     /locus_tag="JQR05_000117"
     CDS             27981..28718
                     /gene="ccmC"
                     /locus_tag="JQR05_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY5322382.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28715..28924
                     /gene="ccmD"
                     /locus_tag="JQR05_000118"
     CDS             28715..28924
                     /gene="ccmD"
                     /locus_tag="JQR05_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY5322383.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28921..29400
                     /gene="ccmE"
                     /locus_tag="JQR05_000119"
     CDS             28921..29400
                     /gene="ccmE"
                     /locus_tag="JQR05_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY5322384.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29397..31340
                     /locus_tag="JQR05_000120"
     CDS             29397..31340
                     /locus_tag="JQR05_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY5322385.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31337..31894
                     /gene="dsbE"
                     /locus_tag="JQR05_000121"
     CDS             31337..31894
                     /gene="dsbE"
                     /locus_tag="JQR05_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY5322386.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            31891..32943
                     /locus_tag="JQR05_000122"
     CDS             31891..32943
                     /locus_tag="JQR05_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY5322387.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33154..33801)
                     /gene="narP"
                     /locus_tag="JQR05_000123"
     CDS             complement(33154..33801)
                     /gene="narP"
                     /locus_tag="JQR05_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY5322388.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34121..36712
                     /gene="yejO"
                     /locus_tag="JQR05_000124"
     CDS             34121..36712
                     /gene="yejO"
                     /locus_tag="JQR05_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY5322389.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKMQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(36815..36891)
                     /locus_tag="JQR05_000125"
     tRNA            complement(36815..36891)
                     /locus_tag="JQR05_000125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36855..36857),aa:Pro,seq:ggg)
     gene            complement(36966..38726)
                     /gene="pbgA"
                     /locus_tag="JQR05_000126"
     CDS             complement(36966..38726)
                     /gene="pbgA"
                     /locus_tag="JQR05_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY5322390.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(38746..38973)
                     /locus_tag="JQR05_000127"
     CDS             complement(38746..38973)
                     /locus_tag="JQR05_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY5322391.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            39155..40162
                     /gene="yejK"
                     /locus_tag="JQR05_000128"
     CDS             39155..40162
                     /gene="yejK"
                     /locus_tag="JQR05_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY5322392.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(40301..40585)
                     /gene="rplY"
                     /locus_tag="JQR05_000129"
     CDS             complement(40301..40585)
                     /gene="rplY"
                     /locus_tag="JQR05_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY5322393.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(40710..42470)
                     /locus_tag="JQR05_000130"
     CDS             complement(40710..42470)
                     /locus_tag="JQR05_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY5322394.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALQLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            42620..43315
                     /gene="rsuA"
                     /locus_tag="JQR05_000131"
     CDS             42620..43315
                     /gene="rsuA"
                     /locus_tag="JQR05_000131"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY5322395.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            43343..44533
                     /gene="bcr"
                     /locus_tag="JQR05_000132"
     CDS             43343..44533
                     /gene="bcr"
                     /locus_tag="JQR05_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY5322396.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSV
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            44866..45210
                     /locus_tag="JQR05_000133"
     CDS             44866..45210
                     /locus_tag="JQR05_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322397.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(45214..46803)
                     /gene="yejF"
                     /locus_tag="JQR05_000134"
     CDS             complement(45214..46803)
                     /gene="yejF"
                     /locus_tag="JQR05_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY5322398.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLLVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(46805..47830)
                     /locus_tag="JQR05_000135"
     CDS             complement(46805..47830)
                     /locus_tag="JQR05_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5322399.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITILFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(47830..48924)
                     /locus_tag="JQR05_000136"
     CDS             complement(47830..48924)
                     /locus_tag="JQR05_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY5322400.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(48925..50739)
                     /locus_tag="JQR05_000137"
     CDS             complement(48925..50739)
                     /locus_tag="JQR05_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY5322401.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVKFTFQKFLTEGMPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDCLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(50821..52377)
                     /locus_tag="JQR05_000138"
     CDS             complement(50821..52377)
                     /locus_tag="JQR05_000138"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY5322402.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(52558..53124)
                     /gene="mepS"
                     /locus_tag="JQR05_000139"
     CDS             complement(52558..53124)
                     /gene="mepS"
                     /locus_tag="JQR05_000139"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY5322403.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(53536..54249)
                     /gene="lpxT"
                     /locus_tag="JQR05_000140"
     CDS             complement(53536..54249)
                     /gene="lpxT"
                     /locus_tag="JQR05_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY5322404.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALTIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(54288..55274)
                     /locus_tag="JQR05_000141"
     CDS             complement(54288..55274)
                     /locus_tag="JQR05_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY5322405.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(55392..56858)
                     /locus_tag="JQR05_000142"
     CDS             complement(55392..56858)
                     /locus_tag="JQR05_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY5322406.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNVQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYISGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(57081..57653)
                     /gene="yeiP"
                     /locus_tag="JQR05_000143"
     CDS             complement(57081..57653)
                     /gene="yeiP"
                     /locus_tag="JQR05_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY5322407.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            57808..58062
                     /locus_tag="JQR05_000144"
     CDS             57808..58062
                     /locus_tag="JQR05_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY5322408.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(58059..59246)
                     /gene="setB"
                     /locus_tag="JQR05_000145"
     CDS             complement(58059..59246)
                     /gene="setB"
                     /locus_tag="JQR05_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY5322409.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALALAREHADKTGREAVMFSSFLRAQVSLAWVI
                     GPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRR
                     DTLLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGY
                     FAKRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQD
                     LMPGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRI
                     KDV"
     gene            59614..60744
                     /locus_tag="JQR05_000146"
     CDS             59614..60744
                     /locus_tag="JQR05_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY5322410.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            60744..61682
                     /gene="fruK"
                     /locus_tag="JQR05_000147"
     CDS             60744..61682
                     /gene="fruK"
                     /locus_tag="JQR05_000147"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY5322411.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            61699..63390
                     /gene="fruA"
                     /locus_tag="JQR05_000148"
     CDS             61699..63390
                     /gene="fruA"
                     /locus_tag="JQR05_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY5322412.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            63814..64755
                     /locus_tag="JQR05_000149"
     CDS             63814..64755
                     /locus_tag="JQR05_000149"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY5322413.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            64743..65681
                     /locus_tag="JQR05_000150"
     CDS             64743..65681
                     /locus_tag="JQR05_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY5322414.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            65775..67025
                     /locus_tag="JQR05_000151"
     CDS             65775..67025
                     /locus_tag="JQR05_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY5322415.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLLFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAILPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(67096..67794)
                     /gene="yeiL"
                     /locus_tag="JQR05_000152"
     CDS             complement(67096..67794)
                     /gene="yeiL"
                     /locus_tag="JQR05_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY5322416.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKHLSGLA
                     LEMDPENKFSGMMQ"
     gene            67925..68866
                     /gene="rihB"
                     /locus_tag="JQR05_000153"
     CDS             67925..68866
                     /gene="rihB"
                     /locus_tag="JQR05_000153"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY5322417.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVVGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            68966..70216
                     /locus_tag="JQR05_000154"
     CDS             68966..70216
                     /locus_tag="JQR05_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY5322418.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(70272..71361)
                     /locus_tag="JQR05_000155"
                     /pseudo
     CDS             complement(70272..71361)
                     /locus_tag="JQR05_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(71364..72221)
                     /gene="nfo"
                     /locus_tag="JQR05_000156"
     CDS             complement(71364..72221)
                     /gene="nfo"
                     /locus_tag="JQR05_000156"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY5322419.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(72295..73344)
                     /locus_tag="JQR05_000157"
     CDS             complement(72295..73344)
                     /locus_tag="JQR05_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY5322420.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            73443..74324
                     /locus_tag="JQR05_000158"
     CDS             73443..74324
                     /locus_tag="JQR05_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5322421.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            74530..75999
                     /gene="lysP"
                     /locus_tag="JQR05_000159"
     CDS             74530..75999
                     /gene="lysP"
                     /locus_tag="JQR05_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY5322422.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            76291..>77854
                     /gene="cirA"
                     /locus_tag="JQR05_000160"
     CDS             76291..>77854
                     /gene="cirA"
                     /locus_tag="JQR05_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY5322423.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGIKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYY"
BASE COUNT        18732 a        19050 c        20996 g        19076 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgataa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgatgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaatcg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaaatg tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt
     1801 ccggtgaccg ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt
     1861 actctgcagc cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     1921 acgcttacca gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     1981 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     2041 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     2101 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     2161 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     2221 agtgtcgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     2281 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     2341 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     2401 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     2461 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     2521 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     2581 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     2641 gggcaatact ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     2701 ccagaaacct cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa
     2761 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca
     2821 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     2881 gaaatccatc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     2941 ggtagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     3001 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     3061 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccag cgtacttaat
     3121 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     3181 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     3241 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cacctgaggc agcgaaagcc
     3301 atgaccgtgg cgatacgtac ttttttacag caaaacgcca atcgggaagg tgattgcctg
     3361 accatccccg acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     3421 cgaacgatga ccgcctggac gcaggagctc gtctatgcag gcgatccggt tcattatcat
     3481 ggcagtcgcg ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag
     3541 ggggagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     3601 ggagctccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     3661 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     3721 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     3781 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg
     3841 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     3901 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     3961 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     4021 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     4081 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta
     4141 tgctgcggcg ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca
     4201 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     4261 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     4321 gggtacagga actattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac
     4381 cgatctcgat cttcatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     4441 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     4501 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     4561 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     4621 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     4681 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     4741 ctgctgggcg atctgctgac gccaggatat aaccattaac aagcgtcggc agattggtca
     4801 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcttggt taaggcaatg
     4861 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     4921 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     4981 ttgcttcttt tcgtacaacc ctgaaagcct cgcactacat gttcagagca ttggcgttag
     5041 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     5101 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     5161 aacgcacctc tgatgtgatg aaagagctta agtacatcgc cgaaaatcgc ttatcggcag
     5221 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     5281 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     5341 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     5401 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     5461 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     5521 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     5581 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     5641 gtgtcgagca gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg
     5701 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     5761 ttaagggcga tcctcgctgg atgcaggaac gctcttggtt tggctatacg gaagggttcc
     5821 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     5881 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     5941 tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc
     6001 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     6061 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     6121 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     6181 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     6241 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     6301 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     6361 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     6421 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     6481 tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     6541 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     6601 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     6661 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     6721 tgcgtttaca gcaggtcatc tccaacctgt tgagtaatgc cataaaattc accgataccg
     6781 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     6841 ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg
     6901 gaacgggcgt acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     6961 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     7021 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     7081 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     7141 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg
     7201 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggcaga gcggtagtga
     7261 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     7321 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     7381 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     7441 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
     7501 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
     7561 ttagtaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
     7621 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
     7681 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctct
     7741 cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca
     7801 ggaaatcgcg ggattcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
     7861 aatgccagat aagacactga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat
     7921 aatgggaatc gtaggccgga taaggcgttt acgccgcatc cggcaatcgg tgcaaatgcc
     7981 agataagaca ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact
     8041 taaggtcaca gaagagagat aattcagcag ggcgatatcg ttctcgacgc ccagcttcat
     8101 catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat
     8161 ctcggtcact aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag
     8221 acgcttgtca ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg
     8281 ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc
     8341 ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt
     8401 gttgttcata gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat
     8461 taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg
     8521 cgcatccagt ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac
     8581 attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc
     8641 atcggcaata attacgttca tattgttcat gtaataggct accttgctac agcaagctct
     8701 tgacataact gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa
     8761 tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata
     8821 cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag
     8881 tatacagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg
     8941 catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca
     9001 gttgcacttc aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga
     9061 cgcacaattg accaggccca atttcccgta cgtcagactc atcatcgctt aaaagcaagc
     9121 cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta
     9181 atctttcatc gggtgtgata caggttgcac cccaattttc taacaggcga gtgacaatat
     9241 tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt
     9301 cttcctcttc aacttccggg tcagcggcga gcattttgat atgcacagag tagcgtgtac
     9361 caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc
     9421 tcagccagaa tgccagcggg tccgcctttc cataacgatc gttttgggtc tggttgataa
     9481 acgggaagtg caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc
     9541 ggaatgtcag gcggtcttca gaggactcat cctgatcaac ctcaagggtg atttttccca
     9601 attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc
     9661 gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt
     9721 tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga
     9781 acagcaccgt ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttctt
     9841 cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga
     9901 gtttagccgc actctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt
     9961 tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt
    10021 tcttgtttac cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg
    10081 cgacctggct gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa
    10141 tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcagatgc ggcagcaaat
    10201 gatcggcaat tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc
    10261 cgagcggcaa tagtgagact atctcttcat tgattgcccg taaaatgcgc aattcgttat
    10321 tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat
    10381 agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt
    10441 gcaacgtatc cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag
    10501 agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg
    10561 tcccttcttt ctcattatca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac
    10621 tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg
    10681 ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc
    10741 aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca
    10801 acatttctgc acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg
    10861 tgagatcttt aaggggtagg gttgagatca gcaccagact gttatcctga ccattcaggt
    10921 aatacatcga ccacggtaca ttttctgcac cccacaatgt atccagataa gtggacatcc
    10981 gcagagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc
    11041 ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc
    11101 cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc
    11161 aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt
    11221 tcagccaggc gttaacggca ctttgtacca ttacccccat cgtcaccagt aacacaatga
    11281 tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg
    11341 ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc
    11401 agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc
    11461 ccgtgatatt tatcatcctg tatgggggga aatagatgta caagcgccat tttgctgtac
    11521 aaataaccta caaaaagccc aacgaaatta attgcaaata aacaaagatt gctggaaatt
    11581 atgctgatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca
    11641 cagaataatg aaaagtgtgt aaagaagggt aaaaaaaaac gaatgcgagg catccggttg
    11701 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    11761 agcgggagtt attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    11821 cttaagaata agttattgat tttaaccttg aattattatt gcttgatgtt aggtgcttat
    11881 ttcgccattc cgcaataatc ttaaaaagtt cacttgtatt tacattttga aacatctata
    11941 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
    12001 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
    12061 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
    12121 cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga
    12181 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacgacaa
    12241 gtctgtagat ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac
    12301 tgaccagctg accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa
    12361 cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtaggttc
    12421 tttcgactac ggtcgtaact acggcgtagt ttacgacgta acttcctgga ccgacgtact
    12481 gccagaattc ggtggtgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg
    12541 cttcgcaacc taccgtagca ccgacttctt tggtctggtt gacggtctga acttcgctgt
    12601 tcagtatcag ggcaaaaacg gtagcccgga aggtgaaggc atgaccaaca acggtcgtga
    12661 agctctgcgt cagaacggtg acggcgttgg cggttctatt acttatgatt acgaaggctt
    12721 cggtatcggt gctgcagttt ccagctccaa acgtactgat gaccagaact tcggtctgaa
    12781 ccgttatgat gagcgttaca tcggtaatgg cgaccgcgct gaaacctaca ccggtggtct
    12841 gaaatacgac gcgaacaaca tctacctagc tgctcagtac acccagacct acaacgcaac
    12901 tcgcgtaggc aacttgggtt gggcgaacaa agcacagaac ttcgaagctg ttgctcagta
    12961 ccagttcgac ttcggtctgc gtccgtccct ggcttacctg cagtctaaag gtaaaaacct
    13021 gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
    13081 tgcgacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
    13141 ggacgacaac caattcactc gtgacgctgg catcaacact gataacatcg tagctttggg
    13201 tctggtttac cagttctaat ctcgatggat atcgaacaaa gggcctgcgg gccctttttt
    13261 cattgttttc agcgtacaaa cacagttttt tggtgtactc ttgcgaccgt tcgcatgagg
    13321 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggttgccg cgctcttctt
    13381 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttactgttct
    13441 tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg
    13501 cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc
    13561 gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg
    13621 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gggcaaggac
    13681 cgatggtgcg atggatatta ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga
    13741 acaacagccg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
    13801 cttacagcac ttgacggtca tcaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
    13861 ggatttatat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg
    13921 cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag
    13981 ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg attcaaaaac caaccgataa
    14041 agaaaacgcg gttcaggctg tggtggatat caacggtcat gggattagca cctctggcag
    14101 ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg atccgcaaac
    14161 cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgctccga cggcgctgga
    14221 agccgatgcc tgggatactg gcttgatggt actcgggccg gagaaagcca aagaggttgt
    14281 tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct ttaaaacctg
    14341 gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa aattaaagcg caagattgtt
    14401 ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta
    14461 tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta gcccgcgacc
    14521 cgaatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt tgccgtccgt
    14581 cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat gccagcgagg
    14641 cactcgccgc cggatttcgc ccctgcaaac gttgtcagcc agacaaagcc aatccccggc
    14701 aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa
    14761 cgctggaagc cttagccgac caggtggcga tgagtccatt tcatctacat cggttgttta
    14821 aagcgactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
    14881 gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg
    14941 acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc
    15001 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggtcgtt
    15061 gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat gatgacgcga
    15121 cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga
    15181 cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
    15241 cgttaccgct ggatattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
    15301 cgataccttg tggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
    15361 aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc gtaataccct
    15421 gtcatcgagt ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta
    15481 aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
    15541 cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac ggcgttttgc
    15601 ttttaatgct gcggagcaac taatccgcga tattaatgac gttgccagcc agtcgccgtt
    15661 tcgccagatg gtcgctccag gcggatatac catgtcggtg gcgatgacca actgtgggca
    15721 tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
    15781 taaaccgtgg cccgccatgc cacaaagctt tcatgattta tgtcaacgtg cggctacggc
    15841 ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctcctggcgc
    15901 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt
    15961 ttctctgggc ttacccgcga tttttcaatt tggtggcctg aaacgtaatg atccgctcaa
    16021 acgtttgttg ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc ggctgtttta
    16081 tcacggtatt caaccgttga aagcggggtt tcatccactc accaccgact gccgctacaa
    16141 cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc
    16201 gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct
    16261 tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc taagcctcgc
    16321 cagtgcggca ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc
    16381 ggataccagt ctgctggtgt taccggagtt tctgggatta ttgctgctgt tgatggcagt
    16441 cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg
    16501 tagtgaattt atcaagcgga ttctggatac ccacgtcgag cgcattgaac aactcggtag
    16561 cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg cttttgtgcg
    16621 tctgccggaa ctggggcagg ggatcattct cactatcggt tcatcggcgt atctgtggat
    16681 gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcgatcacca tctggggcgg
    16741 ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc ctacgtgaaa ccgaagacaa
    16801 gctgtacacg gattttcaaa ctgtacttga agggcgcaaa gagctgactc tgaaccggga
    16861 acgcgccgag tatgtgttta acaacctcta catccccgat gcgcaagagt atcgccatca
    16921 tattattcgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca tcatgatgct
    16981 gggcgctatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg ataccaacgt
    17041 tgctgcgacc tattcgttga cgctgttatt cctgcgtacg ccgctgcttt cggcggttgg
    17101 cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca aattcgcgct
    17161 cgcgcctttc aaagctgagt ttccgcgccc gcaggcgttt cccaactggc aaacgctgga
    17221 gctgcgtaac gtgacgtttg cttatcagga taacgcgttt tccgttggtc cgattaatct
    17281 caccatcaaa cgtggcgagc tgctgtttct gattggcggc aacggtagcg gaaaatcgac
    17341 gctggcgatg ttgttgacgg gcttgtatca gccacaaagc ggcgaaatct tgctggatgg
    17401 caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg cagtgtttac
    17461 cgatgtctgg ctgtttgatc aactgctggg gccggagggg caacccgcta acccgcaact
    17521 ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg
    17581 tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct
    17641 ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt
    17701 ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta aaactatttt
    17761 cgctatcagt catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa
    17821 tgggcaactt agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg atgccgttgc
    17881 ccggacggca taatattacg acgctgacct ggcggactgc tgccgtcagg tcaatatgct
    17941 aaaaatccct tcatttctaa taaccctata attaatcaac gaaattataa tgtttctaaa
    18001 attagaatat aatttataaa cattatttaa atgttgttac ttaagtgtta ccgttgatgc
    18061 cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag atgaaaaaag
    18121 tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg gcggcaaaag
    18181 cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc attatgagcg
    18241 ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc atggtggagc
    18301 gcctggaggg gggcgcgcag gagagttcga acggctggaa caacgccgga accgggcatt
    18361 ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagcatc agtattgaaa
    18421 aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg caccaggtcg
    18481 agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat atgagctttg
    18541 tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg caacaaagct
    18601 cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag tgggcaccgt
    18661 tagtgatgga agggcgcgat ccgcaacaga aagtagcggc aacacgcaca gaaattggca
    18721 ctgatgtgaa ctatggtgag atcacccgcc agttaattgc ttccttacag aagaaatcta
    18781 acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac gataacacct
    18841 ggaccgttac cgttgccgat ctgaaaaacg gcactgcaca gaacatccgc gccaaatttg
    18901 tctttatcgg cgcgggcggt gcggcgctga agctgttaca ggaatcgggg attccggaag
    18961 cgaaagacta cgccggtttc ccggtgggcg gacagttcct tgtttcggaa aatccagacg
    19021 tggttaatcg ccatctggcg aaagtttacg gtaaagcatc cgttggcgca ccaccgatgt
    19081 cggttccgca tatcgatacc cgtgttctgg acggtaaacg cgtagtgctg tttgggccat
    19141 ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt gtgggatctg atgagttcca
    19201 ccaccacctc taacgtgatg ccgatgatgc acgtcgggct ggataatttc gatctggtga
    19261 aatatctggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagcg ttgaaagagt
    19321 actatccgca agcgaaaaaa gaggactggc gtttgtggca agcggggcag cgtgtgcaga
    19381 ttatcaagcg tgatgccgag aaaggtggcg tactgcgtct gggtaccgaa gtggtcagtg
    19441 accagcaagg gaccattgcg gcgctgctgg gggcatcgcc aggggcgtca accgccgcgc
    19501 cgattatgtt gaatctgctg gaaaaagtat ttggcgatcg cgtttccagc ccgcaatggc
    19561 aggctacgtt gaaagcgatc gttccgtctt atggacgcaa gctgaacggt gatgtagcgg
    19621 caacagaacg cgagttgcag tacaccagcg aagtgctggg gctgaaatac gacaaaccgc
    19681 aagcagcaga tagtacgccg aaaccgcagt tgaaaccgca acccgttcaa aaagaagtgg
    19741 cggatattgc gttgtaatga tgtgccacat ccggcatggt atgccggatg tggcgtatac
    19801 tgataagacg cgccagcgtc gcatcaggca accggctcgg gcgttagatg ttggatgcgg
    19861 cgtaaccgcc ttatccaacc aacggggtgc cgtacttcac aacagtttag cgaacttccg
    19921 cgttgtcaat tttctcttcc gccttccaga cgcggtattt cacatctaca ttgtctggcg
    19981 tatacaccac gatcggcagc ttgctgttgt aacgcagcat tccagcatcg cccagatacg
    20041 cggtgacaaa tttcttctct ttcttgccat ccgggcaggc catcatcgtg gaaaccggcg
    20101 agctaacttt atcaaagaca taataatcat agccccagcc ttccagcgtt ttgctttcca
    20161 gcttcccgcc gagacgatgc aaattgcaat cgacttccag cgtctgaccg attaacagtt
    20221 ctactttcag ggtagattca tcttcttgcg gggttaactg aatcacctga cgcttcatgc
    20281 ctgtttcagc ttgtggataa ggcgcgattt tttccagtgg ctggacgctt tctgccgccc
    20341 aggcggaagt ggtagcgaaa gcggcaaaca atactgcagg tagaatggtc ttcatttttt
    20401 tgctttcctt cttcatttat aggttttcac cgatgatgtc gctgaaggtt aacatattca
    20461 tcagatttat taatttgctt tatgaataac ttaaagaaca ttcagacaat tttacctgct
    20521 cttttagtgt taaattctga tgagggagca aaccagcaga aatatctctg gtgaatataa
    20581 tcgctatata aatatgttta taaccatttg aaatgtgagc aaaagcccgt ttttgccacg
    20641 cttcccgcca ctcttttgat cctgctacag gttttacccc gatcggggta tgcatctttg
    20701 acacatcctt taatatctta gcggctataa aaatggctta ttaattatgc ggtctatttg
    20761 gtcgctctca attttcagag cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc
    20821 ccgtcggggc atactcactg gtcgctggcg caaagccagt aacggtatcc gtccgccctg
    20881 gtccggtgat gaatctcatt ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc
    20941 ttgtgaaaac aacattctgc aacgcggcac gggtggctat ccgagcgtta atttcaaaaa
    21001 taacgagtgc agcttctgtt acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc
    21061 gcgccacacc agggcctggg atttgcagtt caccatcggg gacgcgtgcc tggcgtatca
    21121 gtcagttgaa tgccgccgct gtcaggatag ctgtgagcca atggcaatta tctttcgccc
    21181 gacgctctcc gggatctacc agccgcaact taatagccaa ctttgcaacg gatgtggagc
    21241 atgtgcagcc agctgtccgg tatcagccat aactgcggag tatctccatg cacactaact
    21301 ggcaagtttg cagcctggtc gtgcaggcca aaagcgaacg aatttcagac atcagcaccc
    21361 aacagaacgc ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga
    21421 ttgtggtggt ggaagcagaa gacagcgaaa cgctgatcca aaccattgag tcagtacgca
    21481 acgtagaggg cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag caaggtgagg
    21541 aaacaccatg aaactcagtc gtcgtagctt tatgaaagct aacgccgttg cggccgctgc
    21601 ggcggctgcc ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc
    21661 cattaaatgg gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg
    21721 aacgcagcag gggcgtgtgg tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg
    21781 cctgaactgc attaagggct atttcctgcc caagatcatg tacggtaaag accgtttgac
    21841 gcagccgctg ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat
    21901 cacctgggat caggctttcg atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa
    21961 agggccggaa tcgatcggta tgttcggttc tggtcagtgg actatctggg aaggttatgc
    22021 cgcgtccaag ctgttcaaag cgggcttccg ttcgaacaac atcgacccga acgcacgtca
    22081 ctgtatggcg tcggcagtag ttggctttat gcgtaccttt ggtatggatg agccgatggg
    22141 ctgctatgac gacatcgagc aggctgacgc gtttgtgctg tggggcgcta acatggcgga
    22201 gatgcacccg atcctctggt cacgcattac taaccgtcgt ctctctaacc agaacgtcac
    22261 cgtggcggtg ctttctacct accagcatcg tagcttcgag ctggcggata acggcatcat
    22321 ctttacgccg caatctgacc tggtgatcct gaactacatc gccaactata tcattcaaaa
    22381 caatgcgata aatcaggact tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga
    22441 catcggctac ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga atccgggttc
    22501 tgacgcctcc gaaccgatga gctttgaaga ttacaaagcc ttcgttgctg agtatacgct
    22561 ggaaaaaacc gctgaaatga ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct
    22621 gtatgccgat ccgaacaaga aagtcatctc ctactggacg atgggcttca accagcatac
    22681 ccgtggtgtg tgggccaaca acctggtcta caacctgcac ctgctgaccg gcaaaatttc
    22741 ccagccgggt tgcggtccgt tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg
    22801 tgaagtgggc acctttgctc accgtctgcc tgcggacatg gtggtaacta acgagaaaca
    22861 tcgtgatatc tgtgagaaga agtggaatat cccgagcggc accattccgg cgaaaatcgg
    22921 tcttcatgcg gtggcacaag accgtgcgct gaaagacggc aagctgaatg tttactggac
    22981 catgtgtacc aacaacatgc aggccgggcc gaacatcaat gaagagcgta tgccgggctg
    23041 gcgcgatccg cgcaacttca tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc
    23101 cgccgacttg atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc
    23161 cgaacgccgt actcagttct ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga
    23221 tctctggcag ttagtccagt tctcccgccg cttcaaaact gaagaagtat ggccggaaga
    23281 gctgctggcg aagaaaccgg aactgcgtgg caaaacgctg tacgaagttc tgtatgccac
    23341 ccccgaagtg agcaaattcc cggtatccga actggcggaa gatcagctga acgatgaatc
    23401 ccgcgagctg ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg
    23461 cggtcacggt cacgatctgg caccgttcga tgactaccac aaagcgcgcg gtctgcgctg
    23521 gccggtggtg aatggtaaag aaacacagtg gcgttacagc gaaggtaacg acccgtacgt
    23581 gaaagcgggc gaaggctata agttctacgg taaaccggat ggcaaagcgg tgatcttcgc
    23641 actgccgttc gaaccggcgg cggaagcacc ggatgaagag tacgacctgt ggctctctac
    23701 cggacgcgtt ctggagcact ggcacaccgg cagtatgact cgccgtgtgc cggaactgca
    23761 ccgcgccttc ccggaagcgg tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct
    23821 gcgccgtggc gacaaagtga aagtggtttc tcgccgtggc gaagtgatct cgattgttga
    23881 aacgcgcggt cgtaaccgtc cgccacaggg cctggtgtac atgccgttct tcgacgccgc
    23941 acagctggtt aacaaactga cgctggatgc gaccgatccg ctctcgaaag agacggactt
    24001 caagaagtgc gcggtcaaac tggagaaggt gtaagccatt atgtcccggt cagcgaaacc
    24061 tcaaaatggt cgccgccgct ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc
    24121 cgtgggtgtg gcgctggggt tacaacagca aaccgcacgc gcatctggcg tgcggttgcg
    24181 cccgcccgga gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg
    24241 tgttcaggct tgcccttacg acaccttaaa actggcgacg ctggcctctg gtctgtcggc
    24301 gggcacgcca tattttgtcg cacgggatat tccttgcgaa atgtgtgagg acattccgtg
    24361 cgccaaagtg tgcccaagcg gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg
    24421 gatggggctg gcggtactgg tggaccagga aaactgtctc aactttcagg ggctgcgctg
    24481 cgatgtttgt tatcgcgaat gcccgaaaat tgatgaggcc atcaccttgg agctggagcg
    24541 caacacgcgt accggtaagc acgcccgctt tctgccgacg gttcacagcg acgcctgtac
    24601 cggttgcggt aagtgcgaaa aagtatgcgt gctggaacaa ccggcaatca aggtgttacc
    24661 gctgtcactg gcgaaagggg agttaggtca ccattaccgc ttcggctggc tggaggggaa
    24721 caatggcaaa tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc
    24781 gcagtcaccg ttggctggtg ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt
    24841 tgagcggtcc atggtttggt gtgtggatct tgcacggcaa ctacagcagt agcctgttat
    24901 tcgacaccgt tccgctgacc gatccgctga tgacgctgca aagtcttgcc agtggtcatc
    24961 tgcctgccac ggtggcgttg accggtgcgg tgattatcac cgtgctctac gccctggcgg
    25021 gaaagcgatt attttgcagc tgggtctgcc cgctgaaccc gattaccgac ttagcgaact
    25081 ggttacgcag gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac attcgctacg
    25141 tgctgttggt ggtcattctg gtgggatcag ccttaactgg cacgctcatt tgggaatgga
    25201 tcaaccccgt ttctctgatg gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc
    25261 ttattctcgc gctgttttta tttgatctac tggtcgttga acacggctgg tgcgggcaca
    25321 tttgtccggt tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg
    25381 ccagcgatcg gcagaaatgt aaccgctgta tggattgttt tcatgtttgc ccggaaccgc
    25441 atgtgctacg tgccccggtg ctggatgaac aaagcccggt gcaggtcacc agccgcgatt
    25501 gcatgacgtg cggtcgctgc gtggatgtct gttctgagga tgtatttaca ataactacac
    25561 gatggagttc gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg
    25621 gacggcgatg ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt
    25681 tagccagtcg ccggaagtat ccgggacgca ggaaggggcc attcggatgc caaaagagca
    25741 ggatcggatg ccgctgaact atgtgaatca gccgccaatg atcccgcata gcgttgaagg
    25801 ttatcaggta acgaccaata ccaatcgctg cttgcagtgc cacggtgtcg aaagctatcg
    25861 caccactggc gcgccgcgta tcagtcctac tcactttatg gatagcgacg gcaaagtggg
    25921 cgcggaagtg gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc aggccgatac
    25981 cgcgccaatc gtggggaata cctttacccc atcaaaaggt tacgggaaat aagaggttat
    26041 tatgggaaat tctgaccgta agcctggtct gattaagcgc ctgtggaaat ggtggcgtac
    26101 ccccagccat ctggcgctgg ggacgctgct gttgatcggt tttgttggcg gcatcgtctt
    26161 ctgggggggc tttaacaccg ggatggaaaa agccaatacc gaagagttct gcattagctg
    26221 ccacgaaatg cgcaacacgg tgtatcagga atacatggat tccgtgcact acaacaaccg
    26281 tagcggcgtc cgtgcgacct gtccggattg tcacgttccg cacgagtttg tgccgaagat
    26341 gatacgcaag ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg ttattgacac
    26401 gccgcagaaa tttgaagctc atcgtctgac gatggcacag aatgagtggc ggcgcatgaa
    26461 ggacaataac tcgcaggagt gccgtaactg tcacaacttc gagtatatgg atacaaccgc
    26521 ccagaaatcg gttgccgcga agatgcatga ccaggcggtg aaagatgggc aaacctgtat
    26581 tgattgccat aaagggatcg cgcacaagct gcccgatatg cgtgaagtcg agccaggttt
    26641 ttaacagggt tattgcgtgg gtatgcttga agccagagag ttactttgtg agcgggatga
    26701 acgaacctta tttagtggct tgtcatttac gctgaacgca ggagagtggg tacaaatcac
    26761 cggtagcaac ggcgcgggga agacaacgct tctccgtttg ctgacggggt tgtctcgccc
    26821 tgacgctggc gaggttctct ggcaagggca gcccttgcat caggtacgcg acagctacca
    26881 tcaaaacctg ttatggatag gccatcagcc ggggatcaaa acccggctga cggcgttaga
    26941 aaatctgcac ttttatcatc gcgatggcga tgccgcacaa tgtctggaag ccctggcgca
    27001 ggccgggctt gccggattcg aagatattcc ggtaaatcaa ctttctgccg ggcaacaacg
    27061 ccgcgtcgct ttagcgcgtc tgtggttgac ccgtgccaca ttatggatcc tcgacgagcc
    27121 ttttaccgcg attgacgtta acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac
    27181 ggagcagggg gggattgtga ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa
    27241 aattcgccgc atttcactga cgcaaacgag ggccgcatga tgttctggcg tattttccgt
    27301 cttgaactgc gcgtagcgtt tcgccatagc gccgaaatcg ccaacccgct gtggttcttc
    27361 ctgattgtaa ttaccctttt tccgctcagt atcggtccgg agccgcaact gctggcgcgt
    27421 attgcaccgg gcattatctg ggttgctgcg ctgctttcat ccttgctggc gctggaacga
    27481 ctgttccgtg acgatttgca ggacggcagt cttgaacaat tgatgttgtt gccgttaccc
    27541 ttgcccgccg ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta
    27601 ctcatccttt cgccactggt agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg
    27661 atggcgctga cgctgctgct gggaacgcct acgcttggct ttctcggtgc accgggcgtg
    27721 gcgctgacag tgggacttaa gcgcggtggt gtgctgctca gcatactggt gttaccgctg
    27781 actatcccat tactcatctt tgccaccgcc gcgatggacg cggcttctat gcatttgccc
    27841 gttgatgggt atctggcaat tttaggcgcg ttgctggcag gcaccgcgac attaagtcct
    27901 tttgcgacgg cggcagcgtt acgaatcagc attcaataac gctggattac tgatttttgt
    27961 tcgagtctgg tatcgaaact atgtggaaaa cactgcatca actggcgatc ccaccacggc
    28021 tgtatcaaat ctgtggctgg tttataccgt ggctggcaat tgccagtgtg gtcgtgctta
    28081 ccgtcggctg gatctgggga ttcggctttg ctccggctga ttatcagcag ggaaatagct
    28141 accgcattat ctacctgcat gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa
    28201 tggcggtggc ggcgtttatt ggccttgtct ggcagatgaa aatggccaac ctggcggtgg
    28261 cggcgatggc ccccattggt gccgtgttta cctttattgc cctggttacc ggctctgcat
    28321 ggggaaaacc gatgtggggc acctggtggg tatgggatgc acgtctgact tctgaactgg
    28381 tgctgctgtt tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc
    28441 tggcgggccg tgcggcaggt atcctggtgc tgattggcgt ggtgaatcta ccgattattc
    28501 attactccgt ggagtggtgg aacaccctgc atcagggatc aacgcggatg cagcaaagta
    28561 tcgatccggc gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg
    28621 ccacgctgac gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt
    28681 gggtgagtga actgatactg aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa
    28741 tgaatttttc gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt
    28801 tattccgctg gtggttttgg tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg
    28861 tgtggcgcaa cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc
    28921 atgaatattc gccgtaaaaa ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg
    28981 ctgactatcg gtctggtgct atatgcgctg cgctcgaata tcgatctctt ttatacgccg
    29041 ggggaaattc tctacggcaa gcgtgaaact cagcagatgc cggaagtcgg tcagcgtctg
    29101 cgcgttggcg ggatggtgat gccgggtagc gtgcagcgcg atcccaattc gctgaaagtg
    29161 accttcacca tttacgatgc tgaaggctca gtggatgtct cttacgaagg cattttgccg
    29221 gatctgttcc gtgaagggca gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat
    29281 atcctcgcga aagaagtgct ggcgaaacac gacgaaaact acacgccgcc agaagttgag
    29341 aaagcgatgg aagctaacca ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga
    29401 tgccggaaat tggaaacggg ctgctgtgct tggcgttagg gattgcgctg ctgctgtccg
    29461 tgtatccgct atggggcgtg gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt
    29521 ttgcctggct gctgtttatg tctgtggctg gcgcatttct ggtgctggtc aatgctttcg
    29581 tggtcaacga ctttaccgtc acctatgttg ccagcaactc caatacccag cttccggtgt
    29641 ggtatcgcgt ggcggctacc tggggcgcgc atgaaggttc gcttctgctg tgggtgctgc
    29701 tgatgagcgg ctggaccttt gcggtggcga tttttagtca gcgtattccg ctggatattg
    29761 tggcccgtgt actggcgata atggggatgg tcagcgtcgg ctttttgctg ttcattctct
    29821 ttacctctaa cccgttctca cgcacgctgc cgaacttccc gattgaaggg cgcgatctta
    29881 acccgctgtt acaggatccg gggctgatct tccatccgcc tctgctttat atggggtacg
    29941 tgggtttctc ggtggcgttt gcttttgcca ttgcttcttt gctgagcggg cgtctggaca
    30001 gcacttatgc gcgttttact cgcccgtgga cgctggcggc gtggattttc ctgacgctcg
    30061 gcattgtgct cggttccgca tgggcctatt acgaactcgg ctggggtggc tggtggttct
    30121 gggatccggt agaaaacgcc tcgtttatgc cgtggctggt ggggactgca ctgatgcact
    30181 cactggcggt cactgaacaa cgcgccagct tcaaagcgtg gacattactg ctggcaatca
    30241 gtgccttctc gttgtgtctg ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg
    30301 tacacgcgtt tgcgtctgat ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg
    30361 tgattggcgg ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa
    30421 acaatgcgct gtggtcgcgg gaatctttgc tgttagcgaa caacgttttg ctggtcgccg
    30481 cgatgctggt ggtgttgctg gggacgctgc tgccgctggt gcacaagcaa cttggactgg
    30541 gcagtatttc gattggcgaa ccgttcttca acaccatgtt tacctggctg atggtgccgt
    30601 ttgcgctact gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa
    30661 tccgcaattt attgattatc gccttcatct ctacgctggt gctgtctctg ctgttgccgt
    30721 ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg gcctgctgga
    30781 ttgcggtgct ggcaactgcg gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct
    30841 tcagttattg ggggatggtg gcggctcacc tggggctggc agtgacaatt gttggcattg
    30901 cctttagcca gaattatagc gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg
    30961 atattcatga atatcgcttc accttccgtg atgtcaaaga ggtgactggc ccgaactggc
    31021 gcggcggtgt ggcgactatt ggcgtaacgc gagagggtaa accggaaacg gtgctgtatg
    31081 cggaaaaacg ttattacaac actgccgggt cgatgatgac cgaagcggca attgacggcg
    31141 gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg
    31201 tgcgtcttta ctacaaacca tttgttcgct ggatttgggc gggcgggctg atgatggcgt
    31261 tgggcggact gctgtgtctg tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa
    31321 ctgcgccgga ggccgtatga agcgcaaagt attgttaatt ccgttgatta tcttcctggc
    31381 gattgccgcg gcgctgctgt ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa
    31441 tctggaatcg gcgctcattg gcaagcctgt gccgaagttt cgtctcgaat cactggacaa
    31501 tccggggcag ttttatcagg cggatgtgct gactcagggc aaaccagtac tgcttaacgt
    31561 ctgggcgact tggtgtccga cctgccgtgc ggaacatcaa tatctgaatc agctttcagc
    31621 gcagggcatt cgcgtggtcg gcatgaacta taaagacgat cgccagaagg caatcagctg
    31681 gctgaaagag ctgggcaatc cttacgcgct aagcctgttt gatggcgacg gcatgttagg
    31741 gctggatctc ggtgtctatg gcgcgccaga aaccttcctt attgacggca acggtatcat
    31801 tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct
    31861 gtgggagaaa tacagtaagg aggccgcaca atgaggtttt tattgggcgt gctgatgctg
    31921 atgatctccg gctcagcgct ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag
    31981 gaacaacagt tccgtcagct cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc
    32041 attgccgatt ccaactcgat gattgccacc gacctgcgtc agaaagtgta tgaactgatg
    32101 caggaaggta aaagtaaaaa agagattgtc gattatatgg tggcgcgtta cggcaacttc
    32161 gtcacttacg atccgccgtt aacgccgctg accgtgctgc tgtgggtgct gccagtagtg
    32221 gctattggca ttggcggttg ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg
    32281 ccggaagcgt ttcctgaaca aagcgtgccg gaaggtaagc gtgccggata tgttgtttat
    32341 ctgccgggta ttgtggtggc gttaattgtg gctggcgtca gctactacca gactggcaat
    32401 tatcagcagg tgaaaatctg gcagcaggcc acggcacagg ctccggcgtt gctggacagg
    32461 gcgctggacc cgaaagccga tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg
    32521 atgcgtactc aactgcaaaa aaatccggga gatatagaag gctggattat gttgggccgc
    32581 gttggcatgg cgctgggtaa cgccagtatc gccaccgatg catacgctac tgcgtatcgc
    32641 ctcgatccga agaacagcga tgccgcactg ggatacgctg aagcgttgac tcgttcatct
    32701 gatcccaacg acaaccgcct cggcggtgaa ctgctacgtc agctggtgag aacggaccat
    32761 agcaatatcc gtgtgctaag catgtatgcg tttaatgcct ttgagcagca gcgatttggc
    32821 gaagccgttg ccgcgtggga gatgatgttg aaactcttac ctgccaacga tactcgccgt
    32881 gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa
    32941 taaaaagagg cccgatggtg atgccatcgg gctagactga tgacaaagtg cgcttagttt
    33001 atgccggatg cggcgtgaac gccttatccg gcctacaaaa ttgtgtaaat tcaataaatt
    33061 gatcgatcca cgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgtcataaa
    33121 tctcagcccg atgataaatc catcgggcta tttttattgt gccccgcgtt gttgcaggaa
    33181 cagaatggtg gccgccacgc gtgagcggac attgagttta cgcagcagat tgcgaatatg
    33241 tacttttact gtctgctcgg aaatattcaa caccgatgca atctgtttat ttgacagccc
    33301 ctgtgccagc tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc
    33361 ttccgcgcca aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt
    33421 gctgcctttc gctccggtac gaatcgcttc cagcaatact tccgggtcgc tgtctttcaa
    33481 cagataaccg tctgcgcctg cgtctatcag cgcaaagaca tcgctggagg catcggatac
    33541 ggtcaggata ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag
    33601 gccactcata cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc
    33661 cagatcgatc gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag
    33721 ctccagtaac tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg
    33781 aaaaggcgtt gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga
    33841 gcgtagcgtt ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg
    33901 tagtgaaaac tacaaccttg tcgttcgctt tggcatcatt ttcattataa agacggaatt
    33961 ttgcatcagc ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt
    34021 ctttaaacag tttttaaaaa ctgactccgg gtatggagct atgggtattt tctgtaccca
    34081 atgcttttaa ctgcaattaa tttcatagga tgaaagctca atgcatcaat ctggttctgt
    34141 ttctctttgt cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc
    34201 attggcatca acagttgagt atggtgagac agttgatggt gttatcctgg aaaaagatat
    34261 ccagctggtt tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat
    34321 aaaagaattt ggtgtaagta gtaatactga aattaacgga gggtatcaat acattgaaat
    34381 gaatggtacc gcagaatact cagtattaaa tgatggttat caaattgttc aaatgggtgg
    34441 cgcggcaaac cagactaccc tcaataatgg tgtgctacag gtttatggcc cagcgaatga
    34501 taccacgatt aaaggcgggc gcttaatcgt tgaaaaagat ggggggaccg tttttgccgc
    34561 tatcgaaaag ggaggactac tggaggttaa agagggggga tttgcgcttg cggtagatca
    34621 gaaagcaggc ggtgctatta aaacaaccac gcgggccatg gaggtattcg gaacaaaccg
    34681 tctcggtcag ttcgatatca agaatggtat tgctaataat atgttgttgg aaaacggcgg
    34741 aagtttgcga gttgaagaaa atgacttcgc ttataatact actgtggata gtggcggctt
    34801 actggaggtt atggatggcg ggactgcaac tggcgttgat aaaaaagcag gcgggaaatt
    34861 aattgtctca acgaatgcgc tggaagtgag tggtacaaac agtaaaggcc aatttagtat
    34921 aaaggatggt gtgtcaaaaa attatgaact ggatgatggt tccgggctca ttgttatgga
    34981 ggacacgcag gccattgaca ctatccttga tgagcatgcc actatgcaat cgctgggaaa
    35041 ggatactggt acgaaaatgc aggcaaatgc ggtatatgat ctcggtcgat cagaccagaa
    35101 tggaagtatc acgtattcct caaaagccat ctctgaaaat atggtaatca acaatggccg
    35161 cgctaatgtc tgggctggca caatggttaa cgtgtcagtc agagggaatg atggcattct
    35221 tgaggtcatg aagccgcaaa taaattatgc acccgctatg ttggtgggta aggtagtggt
    35281 ttctgagggc gcttctttta gaacgcatgg tgccgtggat accagcaaag cggacgtttc
    35341 gctcgaaaat agcgcatgga ccatcattgc cgatatcact acgacgaacc aaaacactcg
    35401 cctcaactta gccaaccttg cgatgtccgg cgcaaatgtg attatgatgg ctgagccagt
    35461 gactcgttca tctgtgacgg caagtgcgga aaatttcatt acgttgacca ccaataccct
    35521 gtcgggaaac ggcaattttt atatgcgtac cgatatggcc aatcatcaga gcgatcagct
    35581 caacgtcacc ggtcaggcaa caggtgattt caaaatattc gtgacggaca ccggtgccag
    35641 cccggcagca ggagatagcc ttacactggt aacaacgggc ggcggtgatg ctgcatttac
    35701 gttgggcaat gccggaggcg ttgttgatat cggtacgtat gaatatacct tgctggataa
    35761 tggcaaccat agctggagcc tcgcagagaa tcgcgcgcaa attacccctt caaccactga
    35821 tgtgctgaac atggcagccg cacaaccgct ggtatttgat gcagaactgg acaccgtgcg
    35881 tgagcgtctt ggtagcgtaa aaggcgttag ttacgatacg gtgatgtgga gttcggcaat
    35941 taacacccgc aacaacgtga ccactgatgc gggagctggt tttgagcaaa cattgacggg
    36001 cctgacgctc ggtatcgata gccgtttctc ccgtgaagaa agcagtacaa ttcgcggctt
    36061 gttctttggt tactctcatt ctgatattgg ttttgatcgc ggcggaaaag gtaatatcga
    36121 tagctatacc ctgggggctt atgccggttg ggagcatcag aacggtgcct atgttgatgg
    36181 ggtggtgaaa gttgaccgtt ttgccaacac catccatggc aagatgagta atggggcaac
    36241 agcgtttggc gattacaata gtaacggcgc gggtgctcat gttgagagcg ggttccgttg
    36301 ggttgacgga ttgtggagtg ttagacccta tctggccttt accggcttta ccacagatgg
    36361 tcaggactac acgttatcaa acggcatgcg cgcggatgtg ggaaataccc ggatattacg
    36421 cgctgaagcg ggaacggcgg taagctatca catggacctg caaaacggta cgacgctgga
    36481 accctggctg aaagccgccg tgcgtcagga atacgccgat tctaaccagg tgaaagttaa
    36541 tgacgatggc aaatttaata atgatgtggc tggaaccagt ggcgtttatc aggctgggat
    36601 aaggtcatcg tttaccccga cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc
    36661 aggggtagaa tcgccgtgga atactcaggc gggtgtggtc tggacgttct gataacagaa
    36721 aataaacagg ctgtgatgtg tcacggtctg tttatcgaat taattgcaga tataaaaaaa
    36781 ccaaccgtaa gggttggttt tttcttggga tttttggtcg gcacgagagg atttgaacct
    36841 ccgacccccg acaccccatg acggtgcgct accaggctgc gctacgtgcc gactcgtggc
    36901 tgctaatact accgttttcc acaccgattg caagtaagat atttcgctaa ctgatttata
    36961 attaatcagt tagcgataaa acgcttctcg tctgtcagca cttgcaataa caaacttaac
    37021 tgtggttttt catctttcac tctttcacca cgtaagttgt aagtgcggta tttaccgtta
    37081 ttgttcagca ccagcgtctt tttcggggtg gtaattgcca gcgtatcgtt atccgcagca
    37141 gtaacccagt aatgacggcg ttgagggttg aacaaatcct gaccttgtga atattcgctg
    37201 gcaggtgtgc tgacatgcag caggcgttgc atcagcgtcg tcatcagatc ggtatgatca
    37261 gtcagagcat taatacgctg cgccggcgtg cctggccagt gaatcactaa tggcacctgc
    37321 agatgaccgt gggaccagtc aaaggtttct tcctcttcgc tcagtggaat accccgaccg
    37381 gcagtgataa tcaccaccgt attgtccagt ttgccagaat cacgcagtgc attgagcacg
    37441 cggttgatct ggtcatcgac attgcctgcc gcccggctat atttccgtgc aaatgcctgc
    37501 tgattgctgt cgtcaatgtt agtgccattg aaagaaaccc acgagaacca gcggttatct
    37561 tcttgtgcgt agcggcccag ccagttgatc cactgcgtgg cggtctgatc gtcagattgg
    37621 gtgcgcacgc tcggcatcga gaaatctgac aacaatgcct ggcgatacag cgggctggta
    37681 aagccatctg atgagaataa ccccagctga tagccttgct gattaagcgc agtaattaat
    37741 gccgcaggcg tacgggtcga cagaatgccg tccatatagc tcggcgagat gccatagaac
    37801 aggccaaaga tgccgttgtc tgtagtgttg ccggagctca tatggcgcgt gaacgaaata
    37861 ttttgctcag caaaacctgc cagcgcaggc atctgcttct cgaagcgtga gtagttcagg
    37921 ccatcgacag taatcaacag cacattctgc ccggtgccca tatcgcgata gcgcagttcg
    37981 cttaacggat actgtacgga aacggcgtct ggattacctt gctcaataag acggcgttga
    38041 tactcctgcg catcaagcag gccatgcttc tcaaggaaac gtcgcgccgt catcgggtac
    38101 gaaagcggca ggttagcgcg ctgcatggtg atcgggcgat agaagttggc atcggcccag
    38161 atatacacca catgcgaggc gataaaggcg ataaataaga atgcggccag cgggcgcgcg
    38221 aagcgtcgac gacgcgtcag gctgcgcagc ttttgccagc tccacgtcgc aaacaccagt
    38281 tcaagcaata aaataaccgg cacgctgatg aacatcagct gccagtcgcg cgccatctca
    38341 ttttcgtctg ggttgataac caactgccag acgatgggat taagatggag atggaaacga
    38401 gtaaagactt cgctatcgat cagtaatagc gtcattcccg ccgtcgccag aatgacggac
    38461 aaaaacctca tcagcctctg ggagccgacg ataaaggtca gcgggaagag gatcagcaag
    38521 taggtggcga acaccaggaa gctgaaatgg ccgataatgc ttacgtagga ataaatgcga
    38581 ccagcaagcg ttgtcggcca gtcggcgata aacaggtaac ggctgccaat gacgagcgaa
    38641 agcagaatat tgaacagtgc aaaccagtgc ccccaactga ccatctggga gactttttca
    38701 cggtagcgct gacgatgagt taccataacc tgttatctgt ttcccttagt gcgctttgtc
    38761 ttcgttgata gaggactgta aggcgcgggc aaaagagttg gcaattgcct ggcgttgggc
    38821 cggggcaatg ctggtgttga taaggttagt gaccatgttg cctaacacca tcagggaaag
    38881 gtcggtcgga gccttatgtt tttccagtac gttgagcagc tctgcgagca attgttcaac
    38941 ctgttcatta ctgtagcggg aaatttgtgg cataaatcga aatcagttag ttgatgaaag
    39001 ggcaacatat taccgtagca acggtttttt ttctgctttt ttatgcgtta aatcattctc
    39061 gttacgctgc cttctgtaaa atgaccgcca tcttttattg cgtcgcttaa ccggcggtgg
    39121 ttgaataccg cccggtctta aaggagagtt tatcatgagt ctggatatca accagattgc
    39181 tctgcaccag cttatcaagc gtgatgagca gaaccttgag ctggtgttgc gcgattcatt
    39241 gctggaaccg acagaaaccg tcgttgagat ggtggcagaa ctgcatcggg tctatagcgc
    39301 caaaaataaa gcttacggct tgtttagcga agagagtgaa ctggcgcaga cgctgcgctt
    39361 acagcgtcag ggcgaagagg actttctggc atttagccgc gcggcaaccg gtcgcctgcg
    39421 tgacgaatta gcgaaatatc cttttgctga cggtggattc gtgctgtttt gccactaccg
    39481 ttatctggcg gtggagtatt tgctggtggc agttctgagc aacctgagca gtatgcgcgt
    39541 taacgaaaat ctcgacatca acccaaccca ttatcttgat atcaatcatg cggatattgt
    39601 tgcgcgtatc gatttaaccg aatgggaaac caatccagag tccacccgct atctcacttt
    39661 cctgaaaggg cgagtagggc gcaaagtggc ggatttcttt atggatttcc tcggggccag
    39721 cgaaggattg aacgcgaaag cgcaaaaccg tgggctgttg caagcggttg atgatttcac
    39781 tgccgaagca caactggata aagcagagcg acagaacgtg cgccagcagg tttatagcta
    39841 ctgtaatgag cagctgcagg caggcgaaga gatcgaactg gaatcgctgt cgaaagagct
    39901 ggcaggcgta agcgaagtga gcttcacgga gtttgcggct gaaaaaggct acgaactgga
    39961 agagagcttc ccggcagacc gtagcacact gcgtcagttg acgaagtttg ccggtagcgg
    40021 cggtggcttg actattaact ttgatgcgat gctgttaggt gaacgcatct tctgggatcc
    40081 agcaaccgat accttgacca ttaaaggcac gccgccgaat ttgcgcgacc aattgcagcg
    40141 ccggacgtct ggcggcaatt aatcggtttt gtgttctgaa tgccagatgc ggcgtgaacg
    40201 ccttatctgg cctgcaaaaa atagcaatac cgtaatcagg cataatctgc agatacaaaa
    40261 aaccccgccg gagcggggtt ttttacaact tattcagcaa ttaagcgcga acgaagtcga
    40321 tgtgctgcag cttcggtttg tacgggtgac gctgtacgtc ctgagcttta actttgattt
    40381 ctttaccgtc aacaacgatg gtcagaactt cgctgtagaa ttcagcttta gcttgcatgt
    40441 tcatgacttt gtcgtgatcc agctcgatag ccagcggtgc ttctttgcca ccgtagatga
    40501 ttgccgggaa cttgttagcg gcacgcaggc ggcggctcgc acccttaccc tgctctttac
    40561 gtacttctgc gttgatagta aacatttctt tctctcttaa attaatcctg ctacaggcga
    40621 cccagcaaca agtaagtgat ctgctttgcg tatgcaaaag cgggcgagat tctatactca
    40681 atcgccttat acatcaatca aaactaccat taaccgcgta attcgtgcgc ccgacgaaaa
    40741 cgaccttcgt aatcgaacac tttttcacgc acttgccagt actgaccttt catgcgggcg
    40801 acgacaaaat ccggatgtcg cagtaaggct tgctgggcga ggatatcggc ggcggtgatc
    40861 cagcgcagag ggatgcccgg tgtgcgcgta tgcgggcgga taaaaagctg ctcgaaggcg
    40921 gtacgctggg cgggcgtttg cagacggaaa cgctcactca catccgcgcc gtcttcatcg
    40981 taataggtga ttttcaacca ttcgcctttc tcgtcgtgcc catgctgcaa agacatgccg
    41041 ctacagcgta ataccagcgc atctttcagt tgtagcgccg cttttaacat atcatccgga
    41101 tcaaccagta ccgtgtcaca ttcgcggcag cggcgagcgg caatatcatt ttctgcgtta
    41161 cattgcgggc aatttttaaa acggaaacgg aagtcacact gttcgcgatg accgtcgtca
    41221 tcttcaaacc atccctgaca gcggcgacca aagtgttcaa tcaatgtccc gtcggcggtc
    41281 gttttccccc aaaaggtgtt ggcaaaaccg caggcagggc agaaaacctg taccggaacg
    41341 ttgtcacttt tgcctttcgg tgtaccaact tccggcgcgt agagatcgtg aggattaccc
    41401 gcataatcaa gaattaaaca atcagtcttg cccggagcga gacgcagacc gcgcccgaca
    41461 atttgttggt aaagactcac tgattcggta gggcgcagaa tggcgataag atcgacgtgc
    41521 ggggcgtcaa atccggtggt cagtactgcg acgttgacca gatagcgaaa acgctgggct
    41581 ttaaaatctt caattaacac atcgcgctca gcgccggggg tgtcgccagt aatcagtgca
    41641 gcatcttcgg caggcagtaa tcccacaatc tcttttgcgt gttcaacggt ggcggcgaaa
    41701 atcattaccc ctttgcgctt ttcggcgaac tccatgatct ggctgatgat atgtggcgta
    41761 atgcgttgct gttttttcag ctcacggttg agatcagctt cgctgaacag cccgttactc
    41821 tgtgcctgca agcggctgaa atcgtattgc actactggca tatccagtcg ttctggcggc
    41881 gtcagataac cgtgtttaat catataacgc agcggcagct cataaatgca gtcacggaaa
    41941 agggctttct catcgccgcg taccatgccg tgataatgga actggtagat ccagcctttg
    42001 cccaatcgaa aaggcgtggc agtcagcccc agcaggcgta agtggggatt cactttagtc
    42061 aggtgagtga ggatttgctg atactggctc tcttcatcgt caccaatacg gtggcattca
    42121 tcgacaatca acagcgaaaa ttcaccctga aaggcatcaa gattacgggc gaccgactga
    42181 acgctgccaa ataccacttt accgtggctc tctttgcgct ttagcccggc ggcaaaaata
    42241 tcggcttcca gccccagcgc ctgatacttt gcatggtttt gcgccaccag ttctttaacg
    42301 tgtgccagca ccagtacgcg accacgcgcc agccgtgcca gttccgctat caccaggctt
    42361 ttacctgcgc cggtgggcag cacgataacg gcaggggttt tatgacgacg aaaatggttg
    42421 agcgtggcat ccacggcttc ttgctgatat gggcggagtg taaaaatcat ggtttcgcgt
    42481 aggtagatta ttctgctaat agtatgccac ggttcttttt cccttgaggg aagttattag
    42541 accgctatac tgggcagaca acagttcaga ttttcgggtc attacccgac ctccgttttt
    42601 tacaggcaaa atcacacaca tgcgacttga taaatttatc gcacagcaac tcggcgttag
    42661 ccgtgctatt gccgggcgtg aaatccgcgg caatcgtgtc accgtcgatg gcgaaatcgt
    42721 tcgtaatgca gcgttcaaac tgctccctga acatgatgtc gcttacgatg gcaacccgct
    42781 ggcgcagcaa cacggtccac gttacttcat gctcaataag cctcagggct atgtttgctc
    42841 cacggacgac ccggatcacc cgacggtgct ctattttctg gatgagccgg tagcgtggaa
    42901 actgcacgcc gcaggacgtc tggatatcga taccaccggt ctggtgctga tgactgatga
    42961 tggtcagtgg tcgcaccgca ttacttctcc gcgccatcat tgcgagaaga cctatctggt
    43021 gacactggaa tcacctgtag ctgacgatac ggcagagcaa tttgctaaag gcgtgcagct
    43081 gcataacgaa aaagatctca ctaagccggc ggtgcttgaa gtgattaccc caacgcaggt
    43141 tcgtctgacc atcagcgaag ggcgttatca tcaggtgaaa cgcatgttcg ccgccgtggg
    43201 taaccacgtg gttgagctgc atcgtgaacg tattggcggt attacgctgg atgctgattt
    43261 agcccccggt gaatatcgtc cgttaactga agaagaaatt gccagcgtcg tctaactttt
    43321 caatgacttt caggagcccg ttgtgaccac ccgacagcat tcgtcgtttg ctattgtttt
    43381 tatccttggc ctgctggcca tgttgatgcc gctgtcgatt gatatgtatc tgcccgcgct
    43441 accggtaatt tcagcgcagt ttggcgtatc ggtgggcagt acgcagatga ccctcagtac
    43501 ttatattctg ggctttgcgt tggggcagtt aatctacggg ccgatggcag acagcttcgg
    43561 gcgtaagccg gtggttcttg gcgggacgct ggtgtttgcc gccgccgcgg tggcgtgtgc
    43621 gttggcacaa accatcgatc agctgattgt gatgcgtttc ttccacgggc tggctgcggc
    43681 tgcggccagc gtggttatca acgctttgat gcgtgatatt tacccgaaag aagagttctc
    43741 gcggatgatg tcgtttgtca tgctggtgac gaccattgca ctgctgatgg caccgatagt
    43801 tggcggctgg gtgctggtgt ggctgagctg gcattacatc ttctggatcc tggcattagc
    43861 ggcgattctg gcttcggcaa tgattttctt cctgattaaa gaaaccttac caccggagcg
    43921 tcgtcagcca tttcacattc gtaccactat tggtaacttt gcggcgctgt tccgccataa
    43981 acgtgtcctg agctacatgc ttgccagtgg tttcagcttt gccgggatgt tctcattctt
    44041 aagcgccgga ccgtttgttt atattgaaat taaccacgtc gcgccggaaa actttggtta
    44101 ttactttgcg ctaaacattg tttttctgtt cgtgatgacc atctttaaca gccgcttcgt
    44161 ccgccgcatt ggcgcgttaa atatgttccg ctcggggttg tggatacaat ttattatggc
    44221 agcgtggatg gtcatcagtg cgctgctggg gctgggattc tggtcgctgg tggttggcgt
    44281 tgcggcgttt gtgggctgcg tgtcgatggt gtcatccaat gcgatggcgg tcattcttga
    44341 tgagtttccc catatggcgg gaacggcatc ttcgctggca ggaaccttcc gttttggcat
    44401 aggggcaatt gttggcgcat tgctttctct tgcgaccttt aactctgcat ggccgatgat
    44461 ttggtcaatt gcattctgcg caaccagctc cattctcttc tgtctgtacg ccagtcggcc
    44521 gaaaaaacgg tgatctattg cacaacgagg aaggtaaaag gcttcctttg ttgatgcatg
    44581 tcaaccacaa atctatcatt tccccgatat atgtttattt tatgtaaaat caatttatgt
    44641 aaaaagtcac atcattgtag ttaaaaaggt tgagttagat cgcagaaacg ggtacatata
    44701 gccccgcaaa cgtgaccacg cccgcagata ttacttaaat cagagccata gaggccacgc
    44761 aggcgaggca tcaatcttta cgatctgtat aaagacggat tgttgatgat gtgttaaaat
    44821 tgatgtaaac aaattgtgaa gtgaatgtac ttccggggaa aataagtgac ttcattacaa
    44881 ctctcaatcg tccatcgact gccgcagaac tatcgctggt ctgctggttt cgcaggttcg
    44941 aaggttgaac cgattccgca aaatggaccg tgcggtgaca acagcctggt ggcgcttaaa
    45001 ttgcttagcc cggatggtga taatgcatgg tcggtgatgt ataaactaag ccaggcgtta
    45061 agcgacatcg aagttccatg ttcggtgctg gagtgtgaag gtgagccgtg cctgtttgta
    45121 aatcgccagg acgagtttgc tgcaacatgc cgattgaaaa attttggtgt ggcaattgcc
    45181 gaaccctttt caaactacaa tcctttttaa gcgtcagctc aacgccagta actgacgcgt
    45241 atactcctgc tgcggtgtgg caaacacgcg cgcgcacggt ccttgttcca ctacttcccc
    45301 ttgtcgcagt acgataacct gatgacataa cgcgcggaca acgtgcaaat cgtggctgat
    45361 aaacaaatag gccagttgat gcttttgttg caatgatttc aaaagcgtca atatttgcgc
    45421 ctgtaccgtt ttgtcgagtg atgatgtcgg ttcatcaagt atgatcagcg agggcttaag
    45481 aattaatgcc ctggcaatcg caatacgttg tcgctgacca ccagagaact ccgccggata
    45541 acggtggcgt gtttcaggat ctaatcccac ttcatgcatc acggctatca cttgttgttc
    45601 gcgttgtgcg gcagaaagcg tcggctggtg aaccagtaag ccttcctcaa taatctgcaa
    45661 aacgttgagt cgtgggttga gcgaggagtt tggatcctga aataccacct gaatacgatg
    45721 acgagtaggt aacagctggc ggcgatttaa atcttgcagt ggctgaccgt caaagacgat
    45781 gctgccctga gaggtaatca gtcgcagcag cgccagtccc gtcgtgcttt tcccggaacc
    45841 agactcgccc actaaaccca gtgtttcacc cgcccgtagc gtaaaactga tgtttttcac
    45901 caccacatta tgatccacaa tgcgcttcaa aatccctttg cgaatgggga aggcaacctg
    45961 aagctgttca acatccagca acgttgaggc aggttctggc aacggtaccg gatcgcctga
    46021 cggttcactg ttgagtagct tttgtgtgta aggatgagtg ggtgaggcaa atagcgtagc
    46081 ggcgttattt tgctcgacac agcgaccgtt ttgcattacc gccacgcggt gggccagttt
    46141 tctgacaatg ctgaggttat gagtaataaa cagcatgccc atattcagct cgccttgcag
    46201 ttcgcgcaac agctgtaaaa tctgcgcttg gacagagacg tccagtgcgg tggtcggttc
    46261 atcggcaatt aataattccg gtcgcgttaa cagcgccatc gcaatcatca cccgctggcg
    46321 ttcgccgccg gagagctgat gcggataatc tgtcagccgt tttgccgcct ggcggatacc
    46381 gacgcgatca aggcagttaa gaatttcgcc acgagccgct tcccgacgca tcccgcggtg
    46441 gagtgaaagc acttcataaa gctgtttttc cagggtatgc aatggattta acgacaccat
    46501 cggttcctga aaaatcatgg cgatcttatt accgcgtaca ccgcgcaacg tttgatcgct
    46561 ggcatgaagc agcgattcgc catgaaaacg aatatcgccg gagagatatt caaccggcgg
    46621 ggaagggagc aggcgtaaaa ttgacagcgc ggtaacgctt ttgcctgaac ctgactcacc
    46681 caccagcgcc agcgtttcgc cagcctcaat ctgtagtgaa acatcattga ctactgtacg
    46741 tacggtttgc tgatggcgaa aacccaccga caaattttca atcgctaaca gagtttgcgt
    46801 catgctacac cgccttatta ggatcaaatg cgtcgcggac ggcttcacca ataaagatca
    46861 gcaaagacaa taatatcgcc accgacaaga aggcggtgat cccaagccac ggggcctgaa
    46921 ggttattttt cccttgtaac agcagttcac cgagtgacgg tgaaccgagc ggtagaccga
    46981 agccgaggaa atcgagcgag gtcagggtgg ttatcgaact acataaaata aacggtaaaa
    47041 aggtgagggt cgcgaccatg gcattaggca acatatgacg caggatgata ctgcgatcgc
    47101 tgacgccaag cgcctgtgcc gcacgaatgt agtcgaaatt acgagtacgt aaaaactccg
    47161 cccgcaccac gccgaccaga ctcatccagc caaacaagat agtaattgcc agcagccacc
    47221 agaagttagg ctgtacgacg ctggaaagta aaataatcaa aaacagcgtc ggcatccccg
    47281 accatacttc aataaagcgt tgcccccaga gatcgacttt accgccgtaa tagccttgta
    47341 gcgcccccgc cagcacgccc atcacgctgg aacagagagt cagcatcagg ccaaacagaa
    47401 ccgagatccg cgtgccatag agaatgcgtg ccagcacatc gccgccgttt gcatccgttc
    47461 ccagccagtt ttgccgggag ggtggagaag ggaagggctt atcggtagca aagttgatac
    47521 tggtagcacc aaagcgaatc ggtgcccaca gtacccagcc gttattttcc agccgttgtt
    47581 tcagccacgg gtcctgataa tcagcctgac ttgccagcgg gccgccaaaa tcgctttcgc
    47641 tgtagttttt caacaacggg aaataccaac tgccgtcata acgcaccagc aacggtttat
    47701 cgttagcgat aagttcagaa cacaaactca aaccaaacaa aacgaggaaa atccataacg
    47761 accagtagcc gcgacggtta tgacgaaaac gcgcccaacg ggcctgattg acggggctga
    47821 gtcgcgacat taacgtccct caaaatctat acgcggatca accagcgtat agctgatatc
    47881 actgacaata ttcagcagca ggccaatcag ggtgaaaata tacaaggtac caaacattac
    47941 aggataatcg cgcgagacgg tcgcttcgta gcccagtaag ccaagaccat tgagtgaaaa
    48001 catcacctca atcagcagcg agccggtaaa aaacatgctg ataaacgtcg ccggaaaacc
    48061 ggcaatcacc agcagcatgg cgttgcggaa catatgtttc cagagaatgt ttttctcact
    48121 tacccctttc gcacgcgcgg tcaccacgta ttgcttgcgc acttcatcaa ggaatgagtt
    48181 tttggtcagc atggtcagcg ccgcaaagcc accaatcact gtcgccagca ccggtagcgt
    48241 gatatgccac agataatcgg tgattttctg ataccacggc agcgaatcaa agttagcgga
    48301 aaccaggccg cgtagaggga acaggtcgaa atagctgccg cccgcgaaga agacaatcag
    48361 caggatggca aacaaaaagg ccggaatggc gtagccgatg atgataaatg cgctactcca
    48421 gacgtcaaag cgactcccat tataaacagc tttgcgaatg cctaacggaa tcgacaccag
    48481 atagataatc agcgtgctcc acaatccgag ggtgatggaa accggcagac tgtctttaat
    48541 cagcgtcagc accgaggcgc tgcgaaacag gctatcacca aaatcaaagc ggatgtagtc
    48601 ccagagcatt ttgaagtaac gttcgtggat cggcttatcg aaaccgtagc gatgagtgat
    48661 ctcagcgatc acttctggat ctaatccgcg tccgccacgg taattactgt cgctgatatt
    48721 gccgacaccc gtttgcgcat ggctggcacg aacaccttca ccgcctgcgc cgggtaatac
    48781 tccggcatta ccaaactcaa tggcggcgat ggcctgatcg accggaccgc caggcgcaat
    48841 ttgcacgatg aaaaagttga tagtgataat agcccataac gttgggatca ccagcaacag
    48901 acggcgaatc aggtaagcgc ccatctactc tccctgttgt ctggctgacg gcagtttggc
    48961 cgctttatta acgtcatacc accaggtatc gatacccagg ctgtaaacag ggcgtacagc
    49021 gggttgggag aatttatccc accaggcgag acagtcttcc gccatgtacc acattggcag
    49081 catgtaataa ttccacgtta atacgcgatc cagtgctcgc cccaacggca gtaatttttc
    49141 tttatttccc tgcgcggcaa taatttggtt gatcagcgag tcgataaccg ggctttgcac
    49201 gccgggggca ttataagtgg aattgatata ttccgatgac caggaaatct gtaaatcgga
    49261 actgggccac ggcatcgccc gccataggcg cggcatcatg tcatagtcgc gactgcgcat
    49321 gcggttagtg atttgcgagt tatccacctt gcgaatgtcc atattgatac ccagccgttg
    49381 caggctgtgc tggaacggca atacccactg actattgctg cttgcgggaa gcaataattc
    49441 aaagctgagc ggctgacccg ttgtggcatt aacgcgttgc tgccccttca gcacccagcc
    49501 cgcttcgttg agaagtttgt cggcttttaa caggttgtca cgatcgtagc catcgccttt
    49561 agataccggc ggctggtaga tttgtgtgaa gacttctggc ggtagatctt ttttcattgg
    49621 tgccagaagc accagctccg cggcgtcggg gtaatttctg gccgcgtatt cggtattttg
    49681 aaaataactg ttcgtgcgac tccaggcgtt gtaaaacaac gccttgttca tccattcaaa
    49741 gtcaaaggcg agagtgattg cttcccgcac ccggcgatcg ctgaataccg cacgttggat
    49801 attaaacgcc agccagcgcg tatcctgggc tgattcgttc ttttgctcat ctttgatgat
    49861 gtatttttta tcgaaatttt taccggtata acgcgtggcc cagtttttag cgtcgttttc
    49921 catacgcaaa tcaaaggcac ctgctttaaa cgcttcaaag gcgacattat catcgaggta
    49981 ataatcgtag cgaatggtgt cgaaattcca gcgtccacgg tttaccggta agtttgctgc
    50041 ccagtaatct ttcacgcggg aatagacaat attttgcccc attttccagg acgtaacgtg
    50101 gtacggacca ctggcaagcg gaggcgtggc gagcgggtcg ctaagtttgt gatccttcca
    50161 gtacttttct ggaaataccg gcagcgaaaa cagactcaac atatcttctt tgccgggttt
    50221 agctaactca atgcgcacgg ttaacggcgc aatggctttg acggtggtgc ctttgtagac
    50281 cagacgaaat tgcggcatgc cttcggtcag aaatttttga aaagtaaact ttacatcgcg
    50341 ggcagtaatg ggagaaccat catgaaaacg ggcgcgcgga ttaatagcga cctccaccca
    50401 ggaataatcg tcagcatagc gtgcactttc agcaatcagc gggtaataac tgcctggttc
    50461 gtcatcggaa gtcgtaaata acgtgtcgta cagctgctcg gtgcgtgcgc ccgggttgcc
    50521 gcgcagtgca tagcggttga aattatcgaa ggtaccgagg gctgacaacg ttatctgccc
    50581 accttttggc gcggcggggt tcacataatc aaaatggttg aaattaaacg cgtaccgggg
    50641 ttcgcccagc acggcaaagg catagctttc cttgatagcc tgcgcctgcg caccgaaggt
    50701 gaacagagcg ataaacagca gcagtatgcg cacaatcatc tgcaacggat ttccttttac
    50761 tcatcattag ccgcaatccg gcatgctttc actatgaata ataagggacg taagcacacc
    50821 ttaccactgc ggcgtatacg gtttcttcag ccagcgaaca aaatcgtcca gcggtaacgg
    50881 gcgactaatc cagtagcctt gcatgaaatt aacgccgcgt tcgcttagcc atcgcgcttg
    50941 ttccggcgtt tcgacccctt cagtaactgt cagcatattg agacgtttcg ccagcgtcag
    51001 caccgcgtca agtacgggtg aagtgatcgt ttccgtaccg atggcgttga taaatccacg
    51061 gtcaattttc agataatcga gcgtaaaacg ctcaagatag ataagcgcgc tgtgcccggt
    51121 gccgaagtca tcaatagcaa tttctacgcc gaccgagtgc agccaggcga agagttgtgt
    51181 ggcttcttgc tctttcagca tatcgcgctc ggtaatttcc agcacaatct ggaaatggtg
    51241 tgcgggcagg gaagtgagca gtttctggat atctgcttta aagctttcgc tgtgcagatg
    51301 gtccggcgca atgttaatgc caaatttgac gcctaccggc agtacttttt ctaattctgc
    51361 ggcatcgcgg gcaattaact caaacaggtg ctgagtcagc ggcacaatca tcttttgcga
    51421 ttcggcaaag ttaatgaagg catccggggg aatttctccc gctacaggat gccgccagcg
    51481 tagcagtact tccaggcccg ttacccgcaa agcttgtgta tccaccaccg gttgatacgc
    51541 cacgtaaaat tgttcgcgct tgatggcggt catgatttct ctgccggggc gcatacgtac
    51601 gctcatcagg taatagcaaa gcaggccaac gacagttcca ctcatgccgc ccagtaaaaa
    51661 tgcgtaccac acatcgttcc atgtccagtc atccgcataa agccgaacgg tcagaggaat
    51721 gcccgcaatt ttagtttcac ggaccggcat gtcggttaat tcattaacgt tcatcaaacg
    51781 tgaagaaaag gtagatagcg cagtattgcc aataatgagg gcgacgccat cgtaatcttc
    51841 ctgccgtgaa ctataaaaga gagaaggcgt caggttgaga ttcagagcgg caaacacacc
    51901 gctatttttc agcaaagggt tgcgatacca gatgacgatt gcgggtttgt tcggcaccat
    51961 cggcgtgccg ggtaagatcg ccatatcgac gtttttatta atgtcgagcg ccggaatcaa
    52021 ttcattgagt ggaatgtcca tctcaccagt cgcagatgaa cagaatgttt ttttatcttt
    52081 caccagcaca aacgtacgga cattcatgct aaacgccgcg cgagcggtca gttcggggtt
    52141 agcctgctgg caggtatcta aggtcagcgg ctgaagccgg tcagtagtgg atttcaggtc
    52201 ggcaaaatag gtatcgagat acttttgtac gtcgataatc agtgtgtcgt atttgacttc
    52261 gtgcttatgc caggccacta aaaactgaag gcagctaacc agtatcgcaa tcatcacgcc
    52321 tgctacaatg caggtaagca ggagcttacg tccagaattg ggagcgcgta tgaacatgat
    52381 tccgttaatt tcctgaaatt acgacaacat ttaaaagata acaatgaccg gactatagcc
    52441 tggatcaaga cgcggaaaca tcattttctc tgatgaatga aaacaaaaaa gcactgccta
    52501 agcagtgctt tcagttaact cgtcaggata gccaagggat tgcatccaaa cggtttatta
    52561 gctgcggctg agaacccggc gtgcttcgtt gtaacgcttc ttccagtacg gttcattcat
    52621 gctggaaata ataacaccac tgctggtgga agcatggaca aactgattgt tgccgatata
    52681 aataccgaca tggcgtcccg ttgaaccggc acggaacaga actaaatcac ccgtacgcaa
    52741 attactgcgg gagacagatt tacccatttc ctgctgttcg taagtcgaac gcggaagttc
    52801 taagccaaat tgctcacgga atgtacgctg tacgaaacca gaacaatcga tacctttttt
    52861 agtgctgccg cccagacgat aacgtacgcc tttccagtca gcatactgat ccataattcg
    52921 cgattttacg tcgacattac gaaccaggtt ttcaaattca tcctgagaag cttgcagtga
    52981 tgatgtttca ctacccactg cacgtgtctc aggatgcata ttctttgcgg tgttatttgc
    53041 actacatgca gaaagcagaa ccgctactgc aatcgcggga atcccgcgca agatatatct
    53101 caaaatcggt tgagatttga ccatgttgtt tgttttccct tgaagtcctt aacgacaaat
    53161 atcgttataa aaaaatgcca agcgacacga ggctaattac ctgggcatgt tgaaacaatt
    53221 ccttaaggcc aaatttgtgg actaacgcac aaaattatat gctgtgagga taatctaccg
    53281 ccgaggcaaa acgagttcga gattacccga ttggtgcggt cttggcgagc ggttttacat
    53341 gattttttat aagaaaaagt gatacagaaa gttaataagc ggggttggtc aattaaagta
    53401 caattgagtg aaacgcatgc aatccattca tttacttaga gaatgatttt tgcgttcgat
    53461 agatgaaagc acggtgcgca tcatcaggat tatcctcact ataaaaataa ccctgatgat
    53521 gttaattact gtgagttatt tgttttggaa atgtttgttt tttcctggta gtgatttgtc
    53581 aaaaaaggtg attaatcgat cacttaatgg cgtcagcaac acccagggca aaccgatcaa
    53641 tatcacggtc atcgaaccga caatgatgtc agtaaaccag tgtgcgccaa tcattactct
    53701 gggaaatgca aaaaccacaa aaatagtaag ggcgataagg cctgcaactt tgccgaaata
    53761 acgccacatg aatgccgaaa aaataagcag catcatgccg tgatcgccgg gaaagctatc
    53821 tcgtgaggca tctttcgtgg gaacagagag cagttcgctg acgcggttaa tatcggtaaa
    53881 agtcaatgtt gggctggccc gttttacagg aattaatgcc tgacccagct ggtttaatac
    53941 cactgcagtt aatagcatga ccagacccat aatcacgata cgtcgtctgc caggggcgtt
    54001 ttctttcagc cagaaactca gcatcaacat acccatcgcc agcagtgaac aaccgtcgaa
    54061 ggcgcgattg ttggtcaatg caaccagcca taaaaaggcc ttactttcga ccagtttctg
    54121 attaaagaaa taaaaaatat ccgcatcaat cggcaaccag aatccatgat taacgggaat
    54181 ataccaggaa agaaacagcg cggggccgac aatattcaac aacactattt gcggcaaatt
    54241 tttaatcata ttttctctaa ttatcttact gaaaaacgca ggcaacctta cgcggtagtc
    54301 gctaaacgaa gcttcaacag cgcgctctgc aaggcattcc agtcagcttc gctgctggaa
    54361 atcagctcaa tacggctgtc cggtggcgca acgttttgcg tttcaatgtg caggtcatcg
    54421 ccctgacggt tgattcgtac cagcccttcg ggaatacgca gcacgccttt gacgcgttcc
    54481 accggtgcaa gtcgcgccca ttccagaatg ccaatggtgt cgaataccgt atcagcgtcg
    54541 aatatccagc cgcaggcctg atatccttgc ccgctgttca gactgcgacg ccagcgttga
    54601 tgctctggca ggcttaacgc tgctaaccct tttttcacga catgctgatg agaatgcgcg
    54661 gcgctggcgg gcaactcggc taaattgcga cgcggcaaat ccagaagatg accgtcaact
    54721 ttcccatgct cactgtgaat taattgtcga tcgccaccat tttgctgcca ccaacgctgt
    54781 agcgcttgct cactttcggg cgtcgcacgg tcggatttat tggcgacaat gatgtctgcg
    54841 gcagccagct ggtcacggaa gttttcattg ctggcgcttt tttcgtccag cagcaggcgt
    54901 ggatcgagaa tgcacaaggt ggcgcgcaga tctatccacg gttcatagac tggtgcggtt
    54961 aacagatcga ggatctgttt cggatggccc agcccggtcg gctctatcaa caagcggtct
    55021 ggttttccct gacgcagtaa ggtattcaac cctacctgca tgggtaaacc attaacgcag
    55081 cacatgcagc cgccggggat ctctttcagc aatgcgccgc tatcggcgag caaagcacca
    55141 tcaattccga cttccccaaa ttcattaacc agtaccgccc atttttcgtt gggatcttta
    55201 tgggctaaca gatgaagaat cgacgtggtt ttcccgctgc cgagaaaacc ggtgatgagg
    55261 ttggtcctgg tcacaacggc tccgcaatac tctggtaagt taactgagcg ttaatcttgg
    55321 ctaaaaaagg cagaaaagag aaggggagag cggacgaagg tgagcttcat ccgctttaac
    55381 cgcagaaatc gttaaatttt gagggtgttt aatagcgcct gatgtgcgcc gaattgtacg
    55441 atttgttgcc aggcttgttc gatggcctgc acaaaatgcg ggttatctgg cagatcatca
    55501 ccgaaaactt cacgcaggga aagcagggcg gttacgcgtt gttcactgct gctgcccgca
    55561 acaagttcgc gaattttatc gctaagcgga tcgcgaacat caatggcatt tccggcatca
    55621 tcaacgccgc tgatgtaacg catccagcca gcaacgccta atgccagcaa cgaccagtcc
    55681 gtttcacgcc ccagatgtat gcgaatacct gccagcatac gttgcggtaa tttctggctg
    55741 ccatccatcg cgatttgcca ggtcttatgt ttcagcgacg gattagcaaa acgtgcaatt
    55801 aacttatccg catattgtgt taaatcgaca tctttaattt gcagtgtcgg cgcttgctca
    55861 tccagcatta atgttctggc agcatagcga aatacgcgat cctgcatgca atcactgata
    55921 tgggcgaatc ctgagaggta acccagataa gcgagaaaag agtggctgcc attaagcata
    55981 cgcagtttca tctcttccca tggcaggaca tcattcacca tttgtacacc tgcgacttcc
    56041 caggcaggac gcccagcgat gaagttatct tcaaccaccc actggataaa cggttcgcag
    56101 ctaatcgcgc agggatcatt tacccccaga tgctggctga tttctgccag tgattcgtcg
    56161 gttgcagccg gaacaatgcg gtcgaccatg gttcccggaa aactgacgtg ttcttttatc
    56221 cacccggcga gttctggcga acgtttttct gccattctca gcaccgcgtt tttcaccaca
    56281 tggccattgt cgggaatatt gtcgcaggag agcacggtaa acggtgtaag gccgcgctca
    56341 cggcggcgtt tcagtgcttc gacgagaata cccggtgcgg agtgaggttc ttcaggggtt
    56401 tgtagatcgt gaataatccg cggattactg gtgtcgagtg caccggtggc cgggtcaata
    56461 caatagcctt tttcggtaat cgtcagggaa acaattgcca cctgtggctc gcaaaatttc
    56521 tcaataattg ccgctaagga atccagtttt gcattaaggc attcgtgaac ggcaccgaca
    56581 attatcacct gattgccgtc cgcacctttc tccagcacgg tatataaatg gttctgtgcg
    56641 cggagctggc tcatcagttg atcaccgctg aacaagctga tttcacagat cccccagtcg
    56701 ccgccctgga cattcagcac acgatcggtc agtaacgcct gatgagcgcg gtgaaaggcg
    56761 ccaaagccaa aatgaacgat acgtgattgc aactgctggc gatcgtagcg tggagcgtgt
    56821 acatgatgcg ggagcgtaac ggaggcaatt gtcttcatag gagtcacgtc ttagtaacga
    56881 taaattaaca atgacagtgt aaagttatat gacagcaatt gaaattggtc tggattgtta
    56941 atgcgatgaa gtgtgagggc gatcaatcaa cgagtgaaaa ttgagggcgt aaggtgttca
    57001 ggaagcgcaa aatagaagat tggtataata aattaaaaaa gggacagcct gagctatccc
    57061 ttttctgcat gcggctgaag ttagtcagca cgccccatat aacggcgttc ttcgatatgg
    57121 atacgaattt tttcgcccgg gcttaagtat tctggtacct gaatcaccag accagtgctc
    57181 aatgtcgccg gtttgttacg ggcgctggca gatgcccctt tgatgcctgg tgcggtttca
    57241 acgatttcca gatcaacggt ctgcggcagc tcaagcgcca gcagttgacc gtcccaggtc
    57301 agcacctgca tgtccggcat gccgccctct ggcataaaca gcaactcttc ttcaatctga
    57361 tctttggtga aggtatacgg ggtatagtct tctttatcca taaagacata ttcgttgcca
    57421 tcgacatagg agaagtcaac gtaacggcgg gtcagcgtca cggtgtcaac gatatcatca
    57481 cctttgaaac gctcttctac tttcagcccg gtacggacat cagaaaaacg cattttgtac
    57541 agcgtagcgg caccgcgggc agtgggcgac tgaatatcaa tatccttagc cagcagcagt
    57601 ttgccgttgt aattcagtac catacctttt ttaatttcgt tcgctcttgg catcgaaaaa
    57661 ttcctgtagt cgaaaatgtc aaaaatatcg cgacaaagta ctcgcgacgg gcctttcggg
    57721 caagtggtat tcgcactttt gctggtgcaa aaaaggtggt actgtgcgcg ctcatcaatc
    57781 cggtggttaa ccttaagaga acaacgtatg gaatgccgtc cgggttgcgg ggcgtgttgc
    57841 actgcccctt caatttccag ccctattccc ggtatgccag atggcaagcc cgccaatacg
    57901 ccctgcattc agcttgatga acagcaacgc tgtaaaattt tcacctcgcc actgcgaccg
    57961 aaagtctgtg caggcttaca agccagcgct gaaatgtgcg gtaactcgcg tcagcaggcc
    58021 atgacctggc ttatcgatct ggaaatgctg accgcgcctt aaacatcttt aatccgcagt
    58081 aagcaaaaca gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc
    58141 cagatctcgg cgacgatgcc cgccacagat cctgcgatga tccagcccac gcgcgacgtg
    58201 ttggtataga gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc
    58261 atcccgatgc cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga
    58321 atggcaggtg aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca
    58381 acgcgcatta agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc
    58441 gggatttcca gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc
    58501 agttcgttga taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc
    58561 aatgtacaaa tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc
    58621 gtgccggtcg ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg
    58681 ccgcaaacaa taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc
    58741 gctaacgcat aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa
    58801 aaagagctga acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca
    58861 agggcaaaca tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat
    58921 agcaaaacaa agtagttgcg attccaggca aaaagggtgc aggccagcac gcctaacagg
    58981 cagcaaaaga caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac
    59041 tgacttacca gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga
    59101 cgggcatgta cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca
    59161 ataccggtga gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg
    59221 ctggagactg cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt
    59281 ttacgctgct tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa
    59341 atgccatttc actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg
    59401 gattttccca tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg
    59461 ctcataactt tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg
    59521 tttcagcgcg atcttgtctt taaccctaag ccaggttggc gctttttttc tcatagaggc
    59581 tgaatcgttt caattcagca agagaggaga actatgttcc agttatccgt acaggacatc
    59641 catccgggcg aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg
    59701 gtgcaggccg gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa
    59761 acctcaacgt tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat
    59821 caggtgctga aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac
    59881 ggtcaggtag cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg
    59941 ctacgccagc tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca
    60001 acaacagcag aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag
    60061 ctcgacaacg aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg
    60121 ctgaacgctg cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc
    60181 atcaatgaac aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc
    60241 aatctgcgta gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa
    60301 acggcagcca tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag
    60361 cgtctcgctg atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca
    60421 acgttgctgg cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag
    60481 tttgtggtgc gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc
    60541 attaaacaat ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca
    60601 aacggacgta gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc
    60661 tttaccgccc agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct
    60721 gctggtcttg gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg
    60781 cttatgacct tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa
    60841 ccaccggtct gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg
    60901 gaattgatgt caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac
    60961 tgttcagcga gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta
    61021 acgttaagct gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggttttgaag
    61081 tcacccccgc cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg
    61141 atatggtctg tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact
    61201 ggatgactcg cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt
    61261 tagtagcagg tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa
    61321 tctgggcagg ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac
    61381 gtgaacaagg tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta
    61441 atgcctccgg cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg
    61501 caggggattc tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac
    61561 acacactgcg tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta
    61621 ttaccgatcg tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact
    61681 gacagcagga gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca
    61741 cgcgcctata tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc
    61801 atcgacaatc cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac
    61861 agcgcgctga acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct
    61921 gagctgttcc tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg
    61981 acagcaccgg ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact
    62041 ggcgtagcac acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc
    62101 tggtgggtga aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag
    62161 gaagtagccg cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa
    62221 tttgctggta aaccgatgta tcgtacctct accggtctgg cgctgaagaa aaccgcgcag
    62281 gaactggata aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg
    62341 gcgaccactg aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc
    62401 tcttacatgc tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt
    62461 ggtatcgaag cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt
    62521 ggttcagcct ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc
    62581 gatcgtccgg gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct
    62641 ggcttcattg gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt
    62701 acgcaactga aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta
    62761 atttccagtc tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc
    62821 attctcgaag ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg
    62881 ggggcgatcc tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg
    62941 tacgcattcg gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg
    63001 gcggcaggta tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa
    63061 ttcgacaaag cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt
    63121 tcggaaggtg caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc
    63181 gtgggtgggg cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg
    63241 cacggtggtc tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg
    63301 gtagcaatta ttgccggtac gctggtggcg ggtttggcct atgctttcct gaaacgtccg
    63361 gaagtggacg cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc
    63421 tgccctgtaa catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga
    63481 atttaaatgg ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt
    63541 tcctcaaggg cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca
    63601 caaattcctc gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct
    63661 gcccattttg cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca
    63721 tgtttaaata tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga
    63781 cagggttggt aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt
    63841 ataggttcgg cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat
    63901 tcaaatccag gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac
    63961 ctggcgttgc tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt
    64021 caatcgctgc taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg
    64081 ccgggagaaa atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt
    64141 gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt
    64201 gaatttattc agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg
    64261 gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgtgg
    64321 aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt
    64381 gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct
    64441 gcctggttcc atcaacatgg cctgaatcga ctggtattga gcatgggcgg cgacggcgtt
    64501 tattacagcg atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt
    64561 aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga
    64621 atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt
    64681 gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca
    64741 gaatgtctga attaaaaatt tcccctgaat tattacaaat ttccccggaa gtgcaggacg
    64801 ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc
    64861 cgttcccaca aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg
    64921 cagtacctgc cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa
    64981 aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac
    65041 cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg
    65101 cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg
    65161 cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg
    65221 ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa
    65281 ccttcggtgt gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta
    65341 ccagctcgtt tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg
    65401 cggtgaaatg gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac
    65461 agtttgcgat gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg
    65521 aagagcaggg cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc
    65581 tgaccggcgg tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg
    65641 cgagtgaaat tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc
    65701 cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg
    65761 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa
    65821 tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac
    65881 tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag
    65941 tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct
    66001 tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta
    66061 tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
    66121 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc
    66181 tcaacatcag caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg
    66241 agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg
    66301 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
    66361 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt
    66421 ttgcacgtat tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgttgt
    66481 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
    66541 tgaaaatcgc cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
    66601 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
    66661 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
    66721 ccaaccttgc tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc
    66781 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta
    66841 tatcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
    66901 tttcggctat tttgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
    66961 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
    67021 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
    67081 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
    67141 cgccagtcct gacaaatgct ttctgttttt aattagatat cctttcttgc ttttaattaa
    67201 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
    67261 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcttgtg agagtaatat
    67321 aaatgctgcc aggcgattca ctaacggaaa tgattgattc tgagttaaag aaacaatatt
    67381 acgataattt ttatgactta aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa
    67441 taacagcgga cggtaatgtt tcatagggag cgcaaggcac caacactctt caatagcctg
    67501 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcgccaataa aacagggagc
    67561 ggcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
    67621 gcctcgcgtc agataaaaca gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg
    67681 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa
    67741 cgcagattca ctcatcaatt gctgcttaag gtcattattg tggatttctt tcacgatgtt
    67801 tatccgctat gagatttctc atatttagca tacgtacgct gttgttaaga ttcaatcatc
    67861 ggcccggtcg aattttcttt gctgtagaaa aaaataacgc aactggaaac agaggaaata
    67921 aacaatggaa aagagaaaaa ttattctgga ttgtgaaccg ggtcatgatg atgctattgc
    67981 aatgatgatg gcagcgaaac atccggcaat agatttatta ggcatcacta ttgtagtggg
    68041 taatcagacg cttgataaaa cattaattaa tggcctgaat gtttgccaga aactggagat
    68101 taatgttccg gtttatgcgg ggatgccgca acccattatg cgtaaacaaa tcgttgccga
    68161 taatattcac ggtgaaaccg gactggatgg cccggtattc gagccgctga cccgccaggc
    68221 agaaagtacc catgcggtga aatatatcat cgataccctg atggcaagcg atggcgatat
    68281 cactctggtg ccggttggtc cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc
    68341 aatcctgccc aaaatccgtg aaattgtgct aatgggcggc gcttacggta caggcaactt
    68401 caccccatct gccgagttca atatctttgc cgacccggaa gccgcccgcg tggtgttcac
    68461 ctccggcgtt ccattagtga tgatgggcct cgatcttacc aaccagaccg tttgcacccc
    68521 ggatgtaatt gctcggatgg aaagggtagg cggccctgcc ggagagctgt tcagcgacat
    68581 catgaacttc actctcaaaa cgcagttcga atactacggc ctggctggcg gcccggtgca
    68641 tgacgccacc tgcattggtt atctgatcaa ccctgatggc attaaaaccc aggatatgta
    68701 cgtcgaagtg gacgtcaaca gcggcccttg ctacgggcgt acggtctgcg acgaactggg
    68761 cgttcttggc aagcccgcta ataccaaagt cggcatcact attgataccg actggttctg
    68821 gggcttagtc gaagagtgcg tgcgcggcta catcaaaacc cattaagtgt tgataacaag
    68881 ccgggacagt atcgcaagat gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc
    68941 aaaatgacat gaagagataa tcactatgga tgtcatgaga agtgttctgg gaatggtggt
    69001 actgctggca attgcgtttt tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt
    69061 tggcgcggcg ttagtgttac aggtcgtgat tggcgggatt atgctttggt taccgccagg
    69121 gcgttgggtc gcagaaaaag tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc
    69181 aggtagcgca tttatcttcg gttctctggt cggaccgaaa atggatacct tatttgatgg
    69241 cgcaggattt atctttggtt tcagggtatt accggcaatt atcttcgtca ctgcactggt
    69301 gagtattctc tactacatcg gtgtgatggg gatcttaatt cgcattctcg gcggtatctt
    69361 ccagaaagct ttaaatatca gcaagatcga gtcattcgtc gcggtcacca ccattttcct
    69421 cgggcaaaac gaaattccgg cgatcgtcaa accctttatc gatcgtctga atcgcaatga
    69481 attatttaca gcgatttgta gtggcatggc ctcgattgct ggttcgacaa tgattggtta
    69541 cgccgcactg ggcgtacctg tggaatattt gctggcggca tcgttaatgg cgatccctgg
    69601 cgggatcttg tttgcccgcc tgttaagccc ggctacggaa tcttcgcagg tttcttttaa
    69661 taacctctct ttcaccgaaa caccgccaaa aagcattatt gaagccgctg cgacaggggc
    69721 aatgaccggg ctgaaaatcg ccgccggtgt agcgacagtt gttatggcat ttgtcgccat
    69781 cattgcgtta attaacggta ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc
    69841 ctcgctggag tccattttag gttacctgct ggccccactt gcgtgggtaa tgggtgttga
    69901 ctggagtgat gcaaatcttg ccgggagttt gattggacag aaactggcaa taaatgaatt
    69961 tgtcgcttat ctcaatttct caccctatct gcaaacggct ggcactctgg atgctaaaac
    70021 cgtggcgatt atttccttcg cgttgtgcgg tttcgctaac tttggttcta tcggggtggt
    70081 ggtgggggcg ttttctgcgg ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt
    70141 acgcgcgctg gcggcggcga cactttctaa tctgatgagt gccaccattg ccgggttctt
    70201 tattggttta gcgtagtgtg caatttatga gccggattag atgcggtcaa gcgtcgaatc
    70261 cggcaacatt gttacaccat tggcactaat gacagcgcgt tatcggcaga cagggtaggg
    70321 ttgtttaagc tgccgctggc gcgtgaaatt gccgcgcaag ccatcgcaaa acgggcgctg
    70381 tcacggaaat tgtttccctc cagaaagcta tataccagac ccgccataaa accatcgtca
    70441 gcaccaaaac tgtctaccgt cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc
    70501 ttttcgctga aataaactga ctcatcgggc aaataaacaa acagttgctg aacaccttgc
    70561 tgatgcaatg cattcactgc ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat
    70621 aaaatttcca gctccggtaa agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg
    70681 attttgcccg ctttgaattc tgaaacggta tcgacaaaca ccgggatctc atcagcgagg
    70741 gtaaagaccc attccagcgc ctcggctgtc aggttgcaat cagccagtac cacgcccgca
    70801 tgacgaagta aatcgcgcga cccgttcagt agttgcggtg tcaactgttc cagcagatgg
    70861 gtatcgttaa tcgccagcac ggtttgatcg tctcgattgg caattgccag atacgtcgat
    70921 gtgctttgac catgcaaacg aacgcagccg gagacattca cgccggcacg gcgcgtttct
    70981 tctaggagca tttcgccata aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg
    71041 cgctaacagc gccagattgt gggcgatatt gcgtccaacg ccgcctgccg agcaatgaat
    71101 tgtaccggga tgagaagccg cttgcgggta atggatatcc gccatcccgc gaatatccat
    71161 attgattgtc cccaccacta cgcagtattc ctgctcggtg agaatgtaac ctttgccttt
    71221 aatccggcct ttgcgcatta aatccataat atgcgccgca acgcgcgaac gactgatttg
    71281 cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat
    71341 ctccttttcc cggttattca tcttcaggct accgcttttt cagtttgttg cgctttcagc
    71401 caggcgatct cttctgccca gatatccggg ttgatggttt caaggatcag cggaatgccg
    71461 tcgaaacggt cgttctgcat aatccagcgg aacgcatcat gaccgatatt gccttcaccg
    71521 aggctatgat ggcggtcaac gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc
    71581 ccgcgcagat acttaaagcc gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg
    71641 gctggagtac gcaaatcata cccggcagcg aaagcatggc aggtatcaat gcagacgccg
    71701 acgcgggatt tatcttccac gccgtcgata atcgccgcga gatgttcgaa tttaaaccct
    71761 aagttactgc cctgaccggc ggtgttttca atcaccgctg ttacaccttg agttttatcc
    71821 agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat cctcttctga aatctgcatc
    71881 agatggctgc cagggtggaa gttgagcaaa gaaagcccca gttgttcgca acgctgcatt
    71941 tcatctataa aggcatcgcg cgatttttcc agagcttcag tgaccggatg tccgaggttg
    72001 atcagataac tgtcgtgggg aagaatttgc gccgatgtgt agtgatattt ttcacaggcg
    72061 gctttgaatt catcgatggt ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt
    72121 ttggtgaaca aggcaaacgc ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc
    72181 agaccgccag cagcactaac gtgcgctcca atgtatttca tgcgaggact cctgttaaac
    72241 ccgctggagg aaaacggtaa tgatagcggg ttaacaggag caagaagtag tggtttatgc
    72301 gatgacgctt tgaatcacat agttaatcgc accaccacca acaatcagcc aggcaaacag
    72361 taccagtgcc atcagcagag gtttcgcccc agcttttttc agcgcgctga cgtgagtggt
    72421 cagacccagc gccgccatcg ccattgccag caggaaggta tccagcgtta ccagcatgtt
    72481 caccacgctc tgcggtaaca ggtggaacga gttaaagatg gcaactacga tgaacaagat
    72541 ggcaaaccac ggaatagtga ttttgctttt ctcgccgctg ttcgccccag acagctgttt
    72601 aacacgcgcc gccagcagga tgaggaacgg agccagcatc atcacgcgca gcattttgga
    72661 aataactgct gcgttttctg catccgggct gatggcatga cctgccgcca ccacctgcgc
    72721 cacttcgtgc acagtagaac caatgtagat accgaaagtt tccggactaa accattgaga
    72781 catcagcgga tatatcgccg ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt
    72841 tgcaacagcc acggttactt tactggcttc cgctttcact accggctcag tcgccagtac
    72901 cgcggcagca ccacagatac tgctaccggc accgatcaac caactggtgt gcttatccag
    72961 accaaacact ttctgcccca ggaagcaagc cagcaggaag gtactggaca gcgtcagcac
    73021 gtcaatgatg atcccactga taccgacatc ggcaatttgc gagaacgtca gacggaagcc
    73081 ataaagaatg atacccagac gtaataaata ttgcttggca aacagcacac caccgtcaca
    73141 gcttttccag atgtgcggat agatggtgtt gcctaaaacc atccccaaca agattgcgag
    73201 ggtgagggca ctaaacccgg cacccgcaac cgcgggaatg gaaccacccc acagggcgac
    73261 cccggtgata actgcactca gggctaaccc cggaataaaa tgccacagtg tacgatgttg
    73321 tttctgtaag gtgatattcg tcataaccct ctcctttaac cgggcataag gttacgacta
    73381 actggtttaa aaataaaatt gattatatat ttataattaa tctttataag tggtaagcga
    73441 ctatgcacat caccctccgg cagttggaag tttttgcaga agtattgaaa agtggatcaa
    73501 ccacccaggc gtcggtgatg ctggcgttgt cgcaatcagc agtgagcgca gccttgactg
    73561 acctggaagg gcagcttggc gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta
    73621 atgaacacgg gcggctgctc tatccgcgag cgttggcact gcttgaacag gcggttgaaa
    73681 tcgaacaact gtttcgcgaa gacaacggcg cgattcgtat ctatgccagt agtaccatcg
    73741 gtaactacat tctgcctgca gttatcgccc gttatcgcca tgattatccg cagttgccga
    73801 ttgaacttag cgttgggaac agccaggacg tgatgcaagc ggtgctggat ttccgcgttg
    73861 atattggctt tattgaagga ccgtgccaca gcactgaaat catttctgaa ccgtggctgg
    73921 aagacgagct ggtggttttc gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct
    73981 tagaacagct ggccgctgcg ccgtggatcc tgcgtgaacg cggttccggc acgcgggaga
    74041 ttgtcgatta tctgttgctg tcacatttac cgaagtttga gatggcgatg gaattaggta
    74101 actccgaggc aatcaaacat gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac
    74161 gtgtgattga agatcaattg caggcaggca cattaagtga agttgcggtc cctctgccgc
    74221 gcctgatgcg tacgttgtgg cgtatacatc atcggcaaaa acacctttcc aacgcgctac
    74281 ggcgctttct ggactattgc gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac
    74341 atgctggtgc tgtgtcgatt tcgtgacgca gcgccttcag catgcattcg ccagaaaaga
    74401 gattggctgc tttacttata atccctgggc gatcatgaag gtgtcttata accgtgtatt
    74461 tctgccggaa ggattgccaa tcgtctgcta caatcgcgcc atcattttta agatggatag
    74521 catttttgta tgggttccga aactaaaact acagaagcgc cgggcttacg ccgtgaatta
    74581 aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt
    74641 gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgctg
    74701 attggcctga tggtttactt cctgatgacc agtctcggtg aattggctgc atatatgccg
    74761 gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg
    74821 ctgggctgga actactggta caactgggcg gtgactattg ccgttgacct ggttgcagca
    74881 cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag tgcgttgttc
    74941 ctcggcgtta tcttcctgtt gaactacatc tctgttcgcg gctttggtga agcggaatac
    75001 tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg
    75061 attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa
    75121 gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct
    75181 ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac
    75241 attccacgcg cggtacgtca ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc
    75301 ctgattatca gcctgattat tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa
    75361 gacatcagcg tcagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgcggcg
    75421 gcggtgatga acgcagttat tctgacggcg gtgctgtcag caggtaactc cggtatgtat
    75481 gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct
    75541 aaattgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt
    75601 ttgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc
    75661 tccgggatga cgggttttat cgcctggctg gggattgcca ttagccacta tcgcttccgt
    75721 cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc
    75781 ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactac
    75841 gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc
    75901 ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca cttcgtacgc
    75961 tacagcgaaa tgaagttccc gcagaacgat aagaaataag tttcctccct tccttgctaa
    76021 gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa
    76081 aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgac gcaatcaaaa
    76141 aaggctgaca aatcagaggc tgttccggct ttctgggatg atcacctgca tacaaataag
    76201 tccaccgtga tgctgccgta cgcaagggga cgtaaagaag atgtgagcga tacccatttt
    76261 attttcgtag ttacctcatg gagatatgga atgtttaggt tgaacccttt cgtacgggtc
    76321 gggctgtgtt tgtccgctat ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc
    76381 gaaacgatgg ttgtcactgc atcttccgtg gaacaaaatc ttaaagatgc acccgccagt
    76441 atcagcgtca ttacccagga agacctgcag cgaaaaccgg tacagaatct gaaggatgtc
    76501 ctcaaagaag tgcctggcgt acaactgacg aacgaagggg ataaccgtaa gggcgttagt
    76561 attcgtggtc tggacagcag ctacaccctg attcttgtcg acggtaaacg cgttaactcc
    76621 cgcaatgccg tcttccgcca caatgatttc gatctgaact ggatcccggt cgattccatc
    76681 gaacgtattg aagtggttcg tggcccgatg tcgtcgctat acggctccga tgcgctcggc
    76741 ggtgtagtga atatcatcac caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc
    76801 gataccacca ttcaggaaca tcgcgatcgc ggtgatacct ataacggtca gttctttacc
    76861 agtggaccat taattgatgg tgtgctggga ataaaagctt acggcagcct ggcaaaacgt
    76921 gaaaaggatg acccgcaaaa ctcaacgacc accgataccg gagaaacgcc gcgtattgaa
    76981 ggattctcca gccgcgacgg caatgtcgaa tttgcctgga caccgaatca aaatcacgat
    77041 tttactgccg gatacggttt cgaccgtcag gatcgtgatt ccgactcgct ggacaaaaac
    77101 cgcctggaac gccagaacta ctccgtcagc cataatgggc gttgggatta cggcaccagc
    77161 gaactgaaat actacggtga gaaagtcgag aacaaaaacc ctggcaacag cagcccgata
    77221 acttccgaaa gcaatacggt cgacggcaaa tacacgttgc cgctgacggc gattaatcag
    77281 tttctcacgg ttggcggtga atggcgtcac gacaaactta gcgatgcggt gaacctgacc
    77341 gggggaacca gctccaaaac gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg
    77401 cggatcttcg agccgctggc gctgacgacc ggcgtgcgta tggacgatca cgaaacctac
    77461 ggtgaacact ggagtccgcg tgcctacctg gtttataacg ccaccgacac cgtaacggtg
    77521 aaagggggct gggcgacggc atttaaagca ccttctctgt tgcaacttag ccctgactgg
    77581 acgagcaatt cctgccgtgg cgcatgtaag attgtgggta gcccggatct gaaaccagaa
    77641 accagcgaaa gttgggagct ggggctttac tacatgggtg aagaaggctg gctggaaggg
    77701 gttgaatcca gcgttatcgt tttccgtaac gatgtgaaag atcgtatcag catcagccgt
    77761 acgtctgacg tcaacgctgc accgggctac caaaactttg ttggttttga gacgggcgct
    77821 aacggacggc gcataccggt atttagctac taca
//
LOCUS       DADKXM010000003        76222 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.003, whole genome shotgun sequence.
ACCESSION   DADKXM010000003 DADKXM010000000
VERSION     DADKXM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.003"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            125..448
                     /gene="iraM"
                     /locus_tag="JQR05_000161"
     CDS             125..448
                     /gene="iraM"
                     /locus_tag="JQR05_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY5322424.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDIKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(551..1801)
                     /gene="icd"
                     /locus_tag="JQR05_000162"
     CDS             complement(551..1801)
                     /gene="icd"
                     /locus_tag="JQR05_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY5322425.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1973..2626
                     /gene="rluE"
                     /locus_tag="JQR05_000163"
     CDS             1973..2626
                     /gene="rluE"
                     /locus_tag="JQR05_000163"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY5322426.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTKRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2636..3097
                     /gene="nudJ"
                     /locus_tag="JQR05_000164"
     CDS             2636..3097
                     /gene="nudJ"
                     /locus_tag="JQR05_000164"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY5322427.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3151..4257
                     /gene="mnmA"
                     /locus_tag="JQR05_000165"
     CDS             3151..4257
                     /gene="mnmA"
                     /locus_tag="JQR05_000165"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY5322428.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGSIIEQRLPLPV"
     gene            4293..4934
                     /gene="hflD"
                     /locus_tag="JQR05_000166"
     CDS             4293..4934
                     /gene="hflD"
                     /locus_tag="JQR05_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY5322429.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4938..6308
                     /gene="purB"
                     /locus_tag="JQR05_000167"
     CDS             4938..6308
                     /gene="purB"
                     /locus_tag="JQR05_000167"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY5322430.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6477..7148
                     /gene="phoP"
                     /locus_tag="JQR05_000168"
     CDS             6477..7148
                     /gene="phoP"
                     /locus_tag="JQR05_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY5322431.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7148..8608
                     /gene="phoQ"
                     /locus_tag="JQR05_000169"
     CDS             7148..8608
                     /gene="phoQ"
                     /locus_tag="JQR05_000169"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY5322432.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAEESMLGGARMEVIFGRQHSAPKDE"
     gene            8684..9805
                     /locus_tag="JQR05_000170"
     CDS             8684..9805
                     /locus_tag="JQR05_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY5322433.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9854..11080)
                     /gene="pepT"
                     /locus_tag="JQR05_000171"
     CDS             complement(9854..11080)
                     /gene="pepT"
                     /locus_tag="JQR05_000171"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY5322434.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEALSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11330..12466
                     /gene="potA"
                     /locus_tag="JQR05_000172"
     CDS             11330..12466
                     /gene="potA"
                     /locus_tag="JQR05_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY5322435.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12450..13307
                     /gene="potB"
                     /locus_tag="JQR05_000173"
     CDS             12450..13307
                     /gene="potB"
                     /locus_tag="JQR05_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY5322436.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13304..14098
                     /gene="potC"
                     /locus_tag="JQR05_000174"
     CDS             13304..14098
                     /gene="potC"
                     /locus_tag="JQR05_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY5322437.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14095..15141
                     /gene="potD"
                     /locus_tag="JQR05_000175"
     CDS             14095..15141
                     /gene="potD"
                     /locus_tag="JQR05_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY5322438.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15297..16118)
                     /gene="cobB"
                     /locus_tag="JQR05_000176"
     CDS             complement(15297..16118)
                     /gene="cobB"
                     /locus_tag="JQR05_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY5322439.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16134..17045)
                     /gene="nagK"
                     /locus_tag="JQR05_000177"
     CDS             complement(16134..17045)
                     /gene="nagK"
                     /locus_tag="JQR05_000177"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY5322440.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17074..18318)
                     /gene="lolE"
                     /locus_tag="JQR05_000178"
     CDS             complement(17074..18318)
                     /gene="lolE"
                     /locus_tag="JQR05_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY5322441.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18318..19019)
                     /gene="lolD"
                     /locus_tag="JQR05_000179"
     CDS             complement(18318..19019)
                     /gene="lolD"
                     /locus_tag="JQR05_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY5322442.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19012..20211)
                     /gene="lolC"
                     /locus_tag="JQR05_000180"
     CDS             complement(19012..20211)
                     /gene="lolC"
                     /locus_tag="JQR05_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY5322443.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20473..21546
                     /locus_tag="JQR05_000181"
     CDS             20473..21546
                     /locus_tag="JQR05_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY5322444.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21674..25120
                     /gene="mfd"
                     /locus_tag="JQR05_000182"
     CDS             21674..25120
                     /gene="mfd"
                     /locus_tag="JQR05_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY5322445.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMVHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25263..26225
                     /gene="ldtC"
                     /locus_tag="JQR05_000183"
     CDS             25263..26225
                     /gene="ldtC"
                     /locus_tag="JQR05_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY5322446.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPIGHHAIRLAAYDGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPVKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26308..26565)
                     /gene="bhsA"
                     /locus_tag="JQR05_000184"
     CDS             complement(26308..26565)
                     /gene="bhsA"
                     /locus_tag="JQR05_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY5322447.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26807..27439
                     /gene="comR"
                     /locus_tag="JQR05_000185"
     CDS             26807..27439
                     /gene="comR"
                     /locus_tag="JQR05_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY5322448.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27501..28040)
                     /locus_tag="JQR05_000186"
     CDS             complement(27501..28040)
                     /locus_tag="JQR05_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY5322449.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(28267..29571)
                     /locus_tag="JQR05_000187"
     CDS             complement(28267..29571)
                     /locus_tag="JQR05_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAY5322450.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29979..30521)
                     /locus_tag="JQR05_000188"
     CDS             complement(29979..30521)
                     /locus_tag="JQR05_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5322451.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30544..31569)
                     /gene="nagZ"
                     /locus_tag="JQR05_000189"
     CDS             complement(30544..31569)
                     /gene="nagZ"
                     /locus_tag="JQR05_000189"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY5322452.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31580..32404)
                     /gene="thiK"
                     /locus_tag="JQR05_000190"
     CDS             complement(31580..32404)
                     /gene="thiK"
                     /locus_tag="JQR05_000190"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY5322453.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(32385..33026)
                     /gene="lpoB"
                     /locus_tag="JQR05_000191"
     CDS             complement(32385..33026)
                     /gene="lpoB"
                     /locus_tag="JQR05_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY5322454.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQLGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33041..33418)
                     /locus_tag="JQR05_000192"
     CDS             complement(33041..33418)
                     /locus_tag="JQR05_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY5322455.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(33421..33780)
                     /gene="hinT"
                     /locus_tag="JQR05_000193"
     CDS             complement(33421..33780)
                     /gene="hinT"
                     /locus_tag="JQR05_000193"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY5322456.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34108..36297
                     /gene="fhuE"
                     /locus_tag="JQR05_000194"
     CDS             34108..36297
                     /gene="fhuE"
                     /locus_tag="JQR05_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY5322457.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDHVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(36357..37790)
                     /gene="ptsG"
                     /locus_tag="JQR05_000195"
     CDS             complement(36357..37790)
                     /gene="ptsG"
                     /locus_tag="JQR05_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY5322458.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38085..38882)
                     /locus_tag="JQR05_000196"
     CDS             complement(38085..38882)
                     /locus_tag="JQR05_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5322459.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38893..39897)
                     /gene="holB"
                     /locus_tag="JQR05_000197"
     CDS             complement(38893..39897)
                     /gene="holB"
                     /locus_tag="JQR05_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY5322460.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39894..40535)
                     /locus_tag="JQR05_000198"
     CDS             complement(39894..40535)
                     /locus_tag="JQR05_000198"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY5322461.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40525..41547)
                     /gene="yceG"
                     /locus_tag="JQR05_000199"
     CDS             complement(40525..41547)
                     /gene="yceG"
                     /locus_tag="JQR05_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY5322462.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGAWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41550..42359)
                     /gene="pabC"
                     /locus_tag="JQR05_000200"
     CDS             complement(41550..42359)
                     /gene="pabC"
                     /locus_tag="JQR05_000200"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY5322463.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISSDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDCEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(42479..43720)
                     /gene="fabF"
                     /locus_tag="JQR05_000201"
     CDS             complement(42479..43720)
                     /gene="fabF"
                     /locus_tag="JQR05_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY5322464.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43807..44043)
                     /gene="acpP"
                     /locus_tag="JQR05_000202"
     CDS             complement(43807..44043)
                     /gene="acpP"
                     /locus_tag="JQR05_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY5322465.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(44254..44988)
                     /gene="fabG"
                     /locus_tag="JQR05_000203"
     CDS             complement(44254..44988)
                     /gene="fabG"
                     /locus_tag="JQR05_000203"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY5322466.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45001..45930)
                     /gene="fabD"
                     /locus_tag="JQR05_000204"
     CDS             complement(45001..45930)
                     /gene="fabD"
                     /locus_tag="JQR05_000204"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY5322467.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45946..46899)
                     /locus_tag="JQR05_000205"
     CDS             complement(45946..46899)
                     /locus_tag="JQR05_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY5322468.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46967..48037)
                     /gene="plsX"
                     /locus_tag="JQR05_000206"
     CDS             complement(46967..48037)
                     /gene="plsX"
                     /locus_tag="JQR05_000206"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY5322469.1"
                     /translation="MKRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48118..48291)
                     /gene="rpmF"
                     /locus_tag="JQR05_000207"
     CDS             complement(48118..48291)
                     /gene="rpmF"
                     /locus_tag="JQR05_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY5322470.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(48343..48864)
                     /gene="yceD"
                     /locus_tag="JQR05_000208"
     CDS             complement(48343..48864)
                     /gene="yceD"
                     /locus_tag="JQR05_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY5322471.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            49063..49647
                     /gene="yceF"
                     /locus_tag="JQR05_000209"
     CDS             49063..49647
                     /gene="yceF"
                     /locus_tag="JQR05_000209"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY5322472.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49759..50718)
                     /gene="rluC"
                     /locus_tag="JQR05_000210"
     CDS             complement(49759..50718)
                     /gene="rluC"
                     /locus_tag="JQR05_000210"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY5322473.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            51291..54476
                     /gene="rne"
                     /locus_tag="JQR05_000211"
     CDS             51291..54476
                     /gene="rne"
                     /locus_tag="JQR05_000211"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY5322474.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASATPARPQPVE"
     gene            complement(54673..55626)
                     /gene="flgL"
                     /locus_tag="JQR05_000212"
     CDS             complement(54673..55626)
                     /gene="flgL"
                     /locus_tag="JQR05_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY5322475.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAVALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55638..57280)
                     /gene="flgK"
                     /locus_tag="JQR05_000213"
                     /pseudo
     CDS             complement(55638..57280)
                     /gene="flgK"
                     /locus_tag="JQR05_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(57346..58287)
                     /gene="flgJ"
                     /locus_tag="JQR05_000214"
     CDS             complement(57346..58287)
                     /gene="flgJ"
                     /locus_tag="JQR05_000214"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY5322476.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKSSEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRHENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(58287..59384)
                     /locus_tag="JQR05_000215"
     CDS             complement(58287..59384)
                     /locus_tag="JQR05_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY5322477.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(59396..60094)
                     /gene="flgH"
                     /locus_tag="JQR05_000216"
     CDS             complement(59396..60094)
                     /gene="flgH"
                     /locus_tag="JQR05_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY5322478.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANVSRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(60147..60929)
                     /gene="flgG"
                     /locus_tag="JQR05_000217"
     CDS             complement(60147..60929)
                     /gene="flgG"
                     /locus_tag="JQR05_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY5322479.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANVLSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(61067..61818)
                     /locus_tag="JQR05_000218"
                     /pseudo
     CDS             complement(61067..61818)
                     /locus_tag="JQR05_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(61838..63766)
                     /gene="flgD"
                     /locus_tag="JQR05_000219"
                     /pseudo
     CDS             complement(61838..63766)
                     /gene="flgD"
                     /locus_tag="JQR05_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(63778..64182)
                     /gene="flgC"
                     /locus_tag="JQR05_000220"
     CDS             complement(63778..64182)
                     /gene="flgC"
                     /locus_tag="JQR05_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY5322480.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSAPRSYQANVEVLNTVKSMVLKTLTLGQ"
     gene            complement(64186..64602)
                     /gene="flgB"
                     /locus_tag="JQR05_000221"
     CDS             complement(64186..64602)
                     /gene="flgB"
                     /locus_tag="JQR05_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY5322481.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64757..65416
                     /gene="flgA"
                     /locus_tag="JQR05_000222"
     CDS             64757..65416
                     /gene="flgA"
                     /locus_tag="JQR05_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY5322482.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            65492..65785
                     /gene="flgM"
                     /locus_tag="JQR05_000223"
     CDS             65492..65785
                     /gene="flgM"
                     /locus_tag="JQR05_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY5322483.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65790..66206
                     /gene="flgN"
                     /locus_tag="JQR05_000224"
     CDS             65790..66206
                     /gene="flgN"
                     /locus_tag="JQR05_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY5322484.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMRQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(66246..67781)
                     /gene="murJ"
                     /locus_tag="JQR05_000225"
     CDS             complement(66246..67781)
                     /gene="murJ"
                     /locus_tag="JQR05_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY5322485.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67891..68814)
                     /locus_tag="JQR05_000226"
     CDS             complement(67891..68814)
                     /locus_tag="JQR05_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY5322486.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68816..69463)
                     /locus_tag="JQR05_000227"
     CDS             complement(68816..69463)
                     /locus_tag="JQR05_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY5322487.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNVVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69474..70058)
                     /gene="rimJ"
                     /locus_tag="JQR05_000228"
     CDS             complement(69474..70058)
                     /gene="rimJ"
                     /locus_tag="JQR05_000228"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY5322488.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            70294..71502
                     /gene="mdtH"
                     /locus_tag="JQR05_000229"
     CDS             70294..71502
                     /gene="mdtH"
                     /locus_tag="JQR05_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY5322489.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            71566..72213
                     /gene="grxB"
                     /locus_tag="JQR05_000230"
     CDS             71566..72213
                     /gene="grxB"
                     /locus_tag="JQR05_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY5322490.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNDYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDSLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            72347..72907
                     /locus_tag="JQR05_000231"
     CDS             72347..72907
                     /locus_tag="JQR05_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5322491.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            73013..74059
                     /gene="pyrC"
                     /locus_tag="JQR05_000232"
     CDS             73013..74059
                     /gene="pyrC"
                     /locus_tag="JQR05_000232"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY5322492.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAEETVRWSVKQ"
     gene            74133..74378
                     /gene="dinI"
                     /locus_tag="JQR05_000233"
     CDS             74133..74378
                     /gene="dinI"
                     /locus_tag="JQR05_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY5322493.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74668..74922
                     /gene="bssS"
                     /locus_tag="JQR05_000234"
     CDS             74668..74922
                     /gene="bssS"
                     /locus_tag="JQR05_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY5322494.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            75037..76155
                     /gene="solA"
                     /locus_tag="JQR05_000235"
     CDS             75037..76155
                     /gene="solA"
                     /locus_tag="JQR05_000235"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY5322495.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
BASE COUNT        18575 a        20225 c        19032 g        18390 t
ORIGIN      
        1 ttgttttata taagcaatcg ttttaattaa tttatttttt gaaggggtaa tatactcata
       61 tgcaaaataa agaaatgaac atccaaatga actatattaa ataccgtggg ataagacata
      121 acaaatgaag tgggtagtaa ttgacacggt aattcaacct acatgcggca tatctttttc
      181 agccatatgg ggtgatataa aaatgatcat ctggtatcaa tctactatat ttcttcctcc
      241 tggaaggata tttacaccgg ttaagcttgg tattatcctt aaggaaaaag aatatcctat
      301 tactatttat aacattgcac cattcaacaa gaatttatgg agtttactca aaagcagtca
      361 agagtgtcct ccaggagaaa gcgaaataac aaataaatgt ttgcataaga gttgtattat
      421 aaaaatatgc ccatatgggc tcaagtaata ggttttatat aaatatgctc gatatgctat
      481 taataatatc cacataaata aaacaacggg cgtgttatac gcccgtttaa atatttaaca
      541 catgtagtga ttacatattc ttgatgatcg catcaccaaa ctctgaacat ttcagcagtt
      601 tagcgccttc catcagacgc tcgaagtcat aggttacggt cttggcgttg atcgcgcctt
      661 ccataccttt aacaatcagg tctgcggctt cagtccagcc catatggcgt aacatcatct
      721 ctgcggagag aataatagag ccagggttta ctttgtcctg accggcgtat ttcggcgcag
      781 taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat gtttgctcca ggggcgatac
      841 cgataccgcc aacctgcgct gccagagcgt cagaaatgta gtcaccgttc aggttcatac
      901 aggcgataac atcatattca gccggacgca gcaggatctg ttgcaggaat gcatcagcaa
      961 tcacgtcttt aatgacgatc tctttgccgg tattcgggtt tttaactttc agccacgggc
     1021 cgccgtcgat cagttcaccg ccaaattctt cacgcgccag ctggtagccc cagtctttaa
     1081 acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac cagggtcaca gagtcacgat
     1141 cgttagcaat tgcgtattcg atcgctgcac gaaccagacg tttggtgcct tcttcagaac
     1201 acggcttaat accgataccg caatgttccg ggaagcgaat tttcttcacg cccatctctt
     1261 cacgcaggaa tttaatcact ttctcggcgt cggcagagtc agctttccat tcgatacccg
     1321 cataaatgtc ttccgagttt tcacggaaga taaccatatc ggtcagttca gggtgtttaa
     1381 ccgggcttgg agtgccctga tagtaacgta ccggacgcag gcagatgtag agatccagtt
     1441 cctgacgcag ggcaacgttg agagaacgaa taccgccacc aaccggagtg gtcagcgggc
     1501 ctttaatggc aacgcgatat tcacgaatca gatcaagggt ttcagcaggc agccagacat
     1561 cctgaccata aacctgtgtg gatttttcac cggtgtaaat ttccatccag gagattttac
     1621 gctcgccttt ataggctttc tcgactgcag cgtcgaccac tttcagcatg gctggggtta
     1681 catctacacc gattccatca ccttcaatgt aagggataat cggattttca ggaacgttga
     1741 gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg aacaactact ttactttcca
     1801 ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc accacgttgc atatgcgttt
     1861 gcgtcctgcg atacggatgc tttagagcaa ttttttgtta atgatttgta attgacttgt
     1921 caatactacc ctattgtttg ccgtcatgaa agatgcgcgt tattaggcta taatgcggca
     1981 attcataatc tctgaaaata ccatgcaaaa aacttctttt agaaatcacc aggttaagcg
     2041 attcagctcg caacgttcta ccaagcgtaa acatgaaaac cagcccacgc gtgtgatcct
     2101 cttcaataaa ccctacgatg ttcttccgca gttcaccgat gaagccggac gcaaaacatt
     2161 aaaagaattc atcccggttc agggcgttta tgcagcaggt cgccttgacc gcgatagcga
     2221 agggttgctg gtgctgacca ataacggcgc gttgcaggcg cgtttaaccc agccgggtaa
     2281 acgcaccgga aaaatctatt atgtgcaggt ggaaggtatt cccacacaag acgcacttga
     2341 agccttgcgc aatggcgtaa ccttaaatga tggccctacc ctgcccgcag gcgcggagct
     2401 ggttgacgaa cccgcgtggt tatggccgcg gaatccacca attcgtgaac gcaaaagtat
     2461 tcccaccagc tggctgaaga tcaccttata tgaaggacgt aatcgccagg tgcgccgcat
     2521 gaccgcccat gttggtttcc ccacgctgcg actgattcgc tatgcgatgg gagattactc
     2581 tttggataat cttgccaatg gcgaatggcg agaagtgaca gattaaggat gcaaaatgtt
     2641 taaaccgcac gttaccgttg cttgcgtggt gcacgcagaa ggcaaatttt tagtcgttga
     2701 agagacgatt aatggtaaag cgttatggaa ccaacctgcc gggcatctgg aagccgatga
     2761 aaccttagtg gaagccgccg cccgtgaact gtgggaagaa accggcatca gcgcgcagcc
     2821 gcaacacttt attcgtatgc atcagtggat tgcgccagat aaaacgccgt ttttgcgttt
     2881 cctctttgcc attgagcttg agcaaatatg cccgacccag cctcatgaca gcgatatcga
     2941 ctgctgccgt tgggtcagcg ccgaagaaat tttacaggcg tcaaatcttc gttcgccgct
     3001 ggtggcggaa agtattcgtt gttatcaaag cgggcaacgt tatccgctgg agatgattgg
     3061 cgattttaac tggcctttta caaagggtgt catctaaaag gctgcgtgat agaatacgcc
     3121 gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa ccgcaaaaaa agtaatcgtc
     3181 ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc tgttgcaaca acagggatat
     3241 caggtcgaag gcctgtttat gaagaactgg gaagaagacg acggtgagga atattgcaca
     3301 gcggcagcgg atctggctga tgcccaggcg gtctgcgaca agctcggcat tgaactgcac
     3361 accgttaact ttgctgccga gtactgggac aacgtcttcg aactgttcct tgccgaatat
     3421 aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca aggagatcaa atttaaagcc
     3481 ttcctcgaat ttgccgccga agatttaggt gccgattata tcgctaccgg tcattacgta
     3541 cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg gtctggacag caataaagac
     3601 cagagctact tcctttatac gctcagccat gagcagattg cgcaaagcct gttcccggtc
     3661 ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag atcttggtct ggtcaccgcg
     3721 aagaaaaaag actctaccgg catttgcttc attggcgaac gtaaattccg cgagttcctg
     3781 ggccgttatc tcccggcgca accgggcaaa atcattaccg tcgatggcga tgaaattggt
     3841 gagcaccagg ggctgatgta tcacactctc ggtcagcgta aaggtctggg tatcggtggc
     3901 accaaagaag gcaccgaaga accgtggtat gtggtggaca aagacgtcga aaacaacatt
     3961 ctggttgtcg ctcagggcca tgaacacccg cggctgatgt ctgtcgggtt gattgcccag
     4021 cagctgcact gggtcgatcg cgaaccattc accggcacta tgcgttgcac ggtaaaaacc
     4081 cgctatcgcc agaccgacat tccgtgcacc gtcaaggcgc tggacgacga tcgcattaaa
     4141 gtgattttcg atgagccggt tgccgccgtg acgccgggcc agtctgccgt cttctataac
     4201 ggtgaagtgt gcctcggtgg cagtattatt gagcagcgtc tgccgctgcc ggtctgatta
     4261 ttatctttac ttaaaacaag gaagcagtga acgtggcaaa gaattactat gacatcaccc
     4321 tcgccctggc cggtatttgt cagtcggcac gcctggtgca acaactcgct caccaggggc
     4381 attgtgatgc cgatgcgcta cacgtctcac tcaacagtat tattgatatg aaccccagct
     4441 cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg cgtcgggctg gaaaccctgc
     4501 tcggcgtgct caatgccagc agttgccagg gcttaaacgc cgaattaacc cgctacacac
     4561 tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa aggcgcgctc gacactctgg
     4621 gcaaccggat caacggcctg caacgccagc tcgaacactt cgatttacag tccgaaacgc
     4681 tgatgagcgc gatggctgct atctatgttg atgtgatcag cccgcttggc ccgcgcattc
     4741 aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt gcaggcgaaa gttcgcgcaa
     4801 ccctgctggc aggcattcgc gccgccgtgc tctggcacca ggtcggcggc ggacgtctac
     4861 aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa acaaattctt gctcatttaa
     4921 ccccggagtt gtgatctatg gaattatcct cactgaccgc cgtttcccct gtcgatggac
     4981 gctacggcga taaagtcagc gcgctgcgcg ggattttcag cgaatatggt ttgctgaaat
     5041 tccgtgtaca agttgaagta cgttggctgc aaaaactggc cgcgcacgca gcgatcaagg
     5101 aagttcctgc ttttgctgcc gacgcaatcg gttaccttga tgcaattgtc gccaatttca
     5161 gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac cactaaccac gacgttaaag
     5221 cggttgagta tttcctgaaa gaaaaagtgg cggagatccc ggaactgcac gcggtttctg
     5281 aattcatcca ctttgcctgt acttcggaag atatcaataa cctctcccac gcattaatgc
     5341 tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg tcaactgatt gatggcatta
     5401 aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc tcgtacccac ggtcagccag
     5461 ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc ctaccgtatg gaacgccagc
     5521 accgccagct taaccaggtg gagatcctcg gcaaaatcaa cggcgcggtc ggtaactata
     5581 acgcccacat cgccgcttac ccggaagttg actggcatca gttcagcgaa gagttcgtca
     5641 cctcgctggg tattcaatgg aacccgtaca ctacccagat tgaaccgcac gactacattg
     5701 ccgaactgtt tgattgcgtt gcgcgcttca acaccattct gatcgacttt gaccgtgacg
     5761 tctggggtta tatcgccctt aaccacttca aacagaaaac cattgctggt gagattggtt
     5821 cttccaccat gccgcataaa gttaacccga tcgacttcga aaactccgaa gggaatctgg
     5881 gcctttccaa cgcggtattg cagcatctgg caagcaaact gccggtttcc cgctggcagc
     5941 gtgacctgac cgactccacc gtgctgcgta acctcggcgt gggtatcggt tatgcgctga
     6001 ttgcgtatca atccaccctg aaaggcgtga gcaaactgga agtgaaccgt gaccatctgc
     6061 tggatgagct ggatcacaac tgggaagtgc tggcagaacc aatccagaca gttatgcgtc
     6121 gctatggcat cgaaaaaccg tacgagaagc tgaaagagct tactcgcggt aagcgcgttg
     6181 acgccgaagg catgaagcag tttatcgatg gtctggcgtt gccagaagaa gagaaagccc
     6241 gcctgaaagc gatgacgccg gctaactata ttggtcgagc tatcacgatg gttgatgagc
     6301 tgaaataagc ctcaaatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6361 tccacttttt cttttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6421 cataaacaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6481 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6541 atgctggtca tcaggtcgat gacgcagaag atgccaaaga agccgattat tatctcaatg
     6601 aacatttacc ggatattgcg attgtcgatc tcggattgcc tgacgaggac ggtctgtcac
     6661 tgattcgccg ctggcgtagc aacgatgttt cactgccgat tctggtatta accgcccgtg
     6721 aaagctggca ggacaaagtc gaagtattaa gtgccggtgc tgatgattac gtgactaaac
     6781 cgtttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     6841 tggcttcaca ggtcatttcg ctccccccgt ttcaggttga tctctctcgc cgtgaattat
     6901 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     6961 tacgcaataa tggcaaagtg gtcagcaaag attcgttaat gctacaactc tatccggatg
     7021 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     7081 aggcacaata tccgcaagaa gtcattacca ccgttcgcgg ccagggctac ctgttcgaat
     7141 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     7201 gttggcaacg gcagcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     7261 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7321 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7381 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7441 gcaacgtgac gtgccctggc tgatgaaaat gatccagcct gactggctga aatcgaatgg
     7501 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7561 gatacagcaa cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc
     7621 ggtggcggta aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt
     7681 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     7741 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     7801 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     7861 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     7921 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     7981 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     8041 gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     8101 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     8161 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     8221 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     8281 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8341 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8401 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8461 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8521 taaaatcgtc gccgaagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8581 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     8641 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     8701 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     8761 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     8821 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     8881 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     8941 aaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     9001 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     9061 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     9121 aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     9181 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     9241 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggttttc gcgcgccaaa
     9301 tacgcgggaa ttaattagcg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9361 ctactacagc gatccggatg ttccacctcg cgctcatcct gcggatgttc tgccgcaaga
     9421 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9481 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9541 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9601 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
     9661 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
     9721 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
     9781 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcagca tcacatgccg
     9841 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
     9901 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
     9961 cagtgaacag gttcgggcaa ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
    10021 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg catcgcctgc tgggcgatat
    10081 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
    10141 ccaattcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
    10201 ttttacgttt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
    10261 cggcccgttc aacggtgcct ttcatgctcg ccaggtgata gaaaccttca tagccttctg
    10321 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10381 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10441 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10501 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10561 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10621 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    10681 taccatcggt ggtaatcaac gtctgaccca gtagctgatg cagcaccggg aacataaccg
    10741 gtgataaagc ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    10801 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    10861 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    10921 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    10981 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    11041 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    11101 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    11161 caagcgggaa tatttatcag cattacctta atgaatataa agctaagcca gtaattcaca
    11221 acaaagattg gcgattcaag cggtttgccg tagaattacc gccctcatta agagtcacca
    11281 aggtggttaa ccacaaaccc cgcatcggta agccatccgt tgcgtttaca tgggacagag
    11341 taaaaaattg aataaacaac cgagttcgct ttcaccgctg gtgcaattgg cgggaattcg
    11401 caaatgcttt gatggtaaag aggtcattcc ccagctggat ctgactatca acaatggcga
    11461 gttcctcacg ctgcttggcc cttctggctg cggtaaaaca accgttcttc gcctgattgc
    11521 aggtctggaa actgttgatt ccggacgcat catgctggat aacgaggaca tcacccacgt
    11581 tccggcggaa aaccgctatg tgaacactgt tttccaaagc tatgcacttt tcccccacat
    11641 gaccgtgttc gaaaatgttg cctttgggtt gcgcatgcaa aaaacccccg ctgctgaaat
    11701 tacgccccgc gtgatggaag ccctgcggat ggtgcagctg gaaaccttcg ctcaacgtaa
    11761 accgcatcag ctctctggtg gtcaacagca acgcgtcgcc attgctcgcg cggtggttaa
    11821 caagcctcgt ctgttgttgc tggatgagtc gctctcagcg ctggattaca aactgcgtaa
    11881 gcaaatgcag aacgagctga aagcgttaca gcgtaagctt ggcattacat tcgttttcgt
    11941 gactcacgac caggaagaag cactcaccat gtcggacagg attgtggtga tgcgcgatgg
    12001 tcgcattgag caagacggca cgccgcgtga aatctacgaa gagccgaaaa acctgtttgt
    12061 tgccgggttc attggcgaaa ttaatatgtt taacgccacg gtgatcgagc gactcgacga
    12121 gcagcgcgta cgcgccaacg ttgaaggccg cgaatgtaat atctacgtta acttcgccgt
    12181 tgaaccgggg caaaaactgc atgttctgct gcgcccggag gacttacgtg ttgaagagat
    12241 taacgacgac aaccacgctg aagggctgat tggttacgtt cgtgagcgta actacaaagg
    12301 catgacgctg gagtcggttg ttgaactgga aaatggcaag atggtgatgg tcagcgaatt
    12361 cttcaatgaa gacgatcctg actttgacca ctctctcgac caaaaaatgg ccattaattg
    12421 ggtagaaagc tgggaggtcg tactggctga tgaagaacac aagtaagttc cagaatgtag
    12481 tgattgtcac tattgtcggt tggcttgtgt tgtttgtctt tctgcccaac ctgatgatca
    12541 ttggcaccag ctttttgacc cgcgacgacg ccagtttcgt caaaatggtc tttacgctgg
    12601 ataactacac gcgtctgctc gatccgctct attttgaagt gctattgcac tcgctgaata
    12661 tggcgctgat cgccaccctc gcctgcctgg tgctgggcta tccatttgcc tggtttctgg
    12721 cgaagctgcc acacaaggtg cgtccgctgc tgctgtttct gctgattgtt ccgttctgga
    12781 ccaactcatt gattcgtatc tacgggctga aaattttcct cagcaccaaa ggctatctca
    12841 acgagttttt gctctggctg ggcgttatcg acacaccaat ccgtatcatg ttcacgccca
    12901 gtgcggtgat tatcggtctg gtttacattc tgctgccgtt tatggtgatg ccgctgtact
    12961 ccagtatcga aaaactggat aagccgttgc ttgaggcggc gcgcgatctc ggtgccagca
    13021 agttacagac ttttatccgc atcattattc cactgacgat gccggggatt attgccggat
    13081 gtctgctggt gatgctgcca gcgatgggcc tgttctatgt gtccgacctg atgggcggtg
    13141 cgaaaaacct gctgatcggt aacgtcatca aggtacagtt cctcaatatt cgtgactggc
    13201 cgtttggtgc agccaccagc attacgctga ctatcgtaat gggcctgatg ttgctggttt
    13261 actggcgcgc ttctcgtttg ctgaataaga aggtggaact cgaatgatcg gtcgactgct
    13321 tcgcggcggt tttatgaccg ctatctacgc gtacctgtat atcccaatca ttattttgat
    13381 tgtgaactcc tttaacagct cgcgctttgg catcaactgg cagggtttta ccaccaaatg
    13441 gtatagcctg ctgatgaaca acgacagcct gttacaggca gcgcagcatt cactaacaat
    13501 ggcggtgttt tcggcgacgt ttgctacgct tatcggttca ctgacggcag ttgcgctgta
    13561 ccgttatcgc tttcgcggta agccgttcgt tagcggaatg ctgtttgtgg taatgatgtc
    13621 accagatatc gtgatggcaa tttcgctgct ggtgctgttt atgctgctgg gtattcagct
    13681 tggcttctgg tcgctgctgt tctcgcatat caccttctgc ctgccatttg tggtggtgac
    13741 ggtgtattcg cgcctgaaag gttttgacgt gcggatgctg gaagcggcga aagatctcgg
    13801 tgccagcgaa tttaccattc tgcgaaaaat cattctgcca ctggcaatgc cagcggtggc
    13861 ggcgggctgg gtgttaagct ttaccctgtc gatggacgat gtggtagttt cttcgttcgt
    13921 caccggacca agttatgaaa ttctgccgtt aaaaatttat tcgatggtca aagtcggcgt
    13981 atcgccggaa gttaacgcgc tggcaaccat attactggtg ctgtcgctgg tgatggttat
    14041 tgccagccag cttattgctc gtgataaaac gaaaggtaac acaggggacg ttaaatgaaa
    14101 aaatggtcac gccacctgct cgcggcgggt gctctggcac tgggcatgag cgccgctcac
    14161 gccgatgaca acaacacgct gtatttctac aactggaccg agtacgtgcc gccaggactg
    14221 cttgaacagt tcaccaaaga aaccggtatt aaggttatct attcgactta cgagtcgaac
    14281 gaaaccatgt acgccaagct gaaaacgtac aaagacggtg cctatgacct ggtggttcct
    14341 tcaacctatt acgtcgataa aatgcgtaaa gaggggatga tccagaagat cgacaagtcg
    14401 aagttaacga acttcagcaa tctcgatcca gacatgctca acaagccttt tgacccgaat
    14461 aacgactact ccattccgta tatctggggt gcgacggcga ttggtgttaa cggtgatgcg
    14521 gtggatccga aatctgtcac cagctgggcc gatctgtgga agccagagta caaaggcagc
    14581 ctgctgttga ccgacgatgc ccgtgaagtg ttccagatgg cgctgcgtaa gctgggctac
    14641 tccggtaaca ccaccgatcc gaaagagatt gaagctgcat ataacgagct gaaaaaactg
    14701 atgccaaacg tcgcagcgtt taactccgat aacccggcta acccgtacat ggaaggcgaa
    14761 gttaacctcg gcatgatctg gaacggttct gctttcgttg cacgccaggc gggtacgcct
    14821 attgacgtgg tgtggccgaa agaaggcggc atattctgga tggacagcct ggcgatcccg
    14881 gcaaatgcca aaaacaaaga aggcgcgctg aaattgatca acttcctgct gcgcccggat
    14941 gtggcaaaac aggttgctga aactatcggt tatccaacgc caaaccttgc ggcgcgtaag
    15001 ctgttaagtc cagaagtggc aaacgataag acgctctacc cggatgctga aaccattaaa
    15061 aatggcgaat ggcagaatga cgttggcgca gccagcagca tttatgaaga gtattatcag
    15121 aagctgaaag caggacgtta attcagcaag cgggcggtgg tattaccgcc cgttttttag
    15181 ttagcaagga tgtggctaat gagtcaggac agtaaagttt tatcccgctc tttcgtcggt
    15241 attgtggtcc gcttgtaggc ctgataagcg tagcgcatca ggcaatgctt cctgctttat
    15301 aagcccttca gcaacttttc aacaaattct ggcaccacct ggcttgccgg gccgtaatat
    15361 ttctcggcaa attcattacc aacctgactc ggttcaagat tcagttcaac ggtgtgagca
    15421 ccatgtagtt tggcttcgtg tacaaaccca gccgccggat agacatgccc ggaagtacca
    15481 atggcaataa agacatccgc catcgacaac gccatataaa tttcatccat gccgagtggc
    15541 atttcgccaa accaaacgac gtgcgggcgc aagggtgccg ggaactggca acaatggcat
    15601 ttatcttctg gcgtaacgtc tccggtccag tcgagaacct gaccactttg cgaacaacga
    15661 actttcagca attccccatg catatgaatg acattggtat tacctgcgcg ttcatgcagg
    15721 ttgtctatat tctgcgttac cagcaaaaag cgatcgccga gagcgtcttg cagtttagcc
    15781 agcgcaagat gcgcggcgtt aggctgaatt tctggctgct gcagctgtcg acgacgggcg
    15841 ttataaaacg cttgcaccag ttcaggatcg cgatcgaaac cttccggcgt tgccacatct
    15901 tcaacccgat gttcttccca caggccatct gcggcgcgaa aggtacgaat acctgattcc
    15961 gcagaaattc ctgcccctgt cagtacgagt actcttggtt tttccattgc ttccggcacc
    16021 actttatctc tgaaaaaaat acgctgacgc aaacgctcgc gcaggcggcg tttattttta
    16081 cgaaaacgac ttaaccgatg accccgacgc gacaacatag caacctctgt tgtttaatcg
    16141 gttagatgta ggaaggccgc accacgcatt cctcccgcat caccgtggcg cgcgcgttca
    16201 atgcgcggaa cacgagctac aggtaagaga tgacaaggca acctgtccgc cagttgcgtt
    16261 gtgattgccg ggaaattcga taagccacca ccaatgacga ccaggtcagg gtcaacaatg
    16321 gtcaggatat ttcccagaga aaccgctaat aaatccagat aacgctcaac gtgcgccctt
    16381 gcctgctcat cgccttgatc ataaagcgca ataatttcgg gagcctgcaa cggttgatga
    16441 taatagtgtt gatacagcca cgcaaaaccg cgaccagaca gataattttc aatgcagcca
    16501 tgctgaccac agccacagcg gcgtaacggg aaatccaacc ccatcatggt taacgcatca
    16561 accggcagac gcatatggcc aaactcgccg gtaatgtagc ttttaccagt aatcggtttg
    16621 ccgttgaaaa tcagcccgcc gccaacgccg gtgccgagaa ttaaccccat caccagtgga
    16681 tattgcgtaa attcatcatc ccaggcttct gaaagggcaa aacagttggc atcgttatca
    16741 aggcgtacat cgcgatcaag acgtgcgctc aggtcggcac gcagcggttt gccgctggca
    16801 gcggggacat tagcggcata cagcttacca tcttctgttt ccggcattcc cggaataccg
    16861 atgccgacag aacctttaca gccaaaacgt tggtcagctt cagctaccag ctcacacact
    16921 gcatctaaaa atgcgtcata gctgtcacgc ggtgtcggca cccgcttttc ccactgcaac
    16981 tgccgaccgc tatcaaacac gccaagcgca atttttgttc caccaatatc aaacccgtaa
    17041 tacattgccg ctccttgttt taatgtactg cctttactgg ccgctaagga ctcgcgcagg
    17101 gtcaatatta ctggcgcgcc gcgccggata ccaacttgcc aaaagactca gcaacaatgc
    17161 tgtgaccagt acgtagaaga cgtccagcca gtgcaattcc gatggcagga agtcaataaa
    17221 atagatatcg ctggagagga actgatgacc gatcagcttt tcaatccact caataatcgg
    17281 ggtaagttgc agtgaaacaa ctacgccgat aaccacccca cacaggctgc cgaacagccc
    17341 tgccagcaat ccataccaga caaagatggc gcgaattaaa ccatctttcg cccccagcgt
    17401 tcttaatact gcgatatcgc cactcttgtc tttcaccgcc atcactaagg tggagacgat
    17461 gttgaaacag gccacgccaa tcaccagtac catcgccaga tacataatgg cgcggatcat
    17521 ctggatatcg cgatacatat agccgtaagt accaatccaa cttttaatat aaacatagct
    17581 gttggtcact tcacccgcat cgcgtaccag cttattggcg ttgaaaacat ccgtcatttt
    17641 aagggcaata cctgacacgc tggaacccat atcaagatat tgttgggcat ccgccagcgg
    17701 gatcatggca aaactgtgat cgagttgacc actcaactgc aaaataccgg caatgtgcaa
    17761 acgcacacgt tttggctgca tcagtttatg ctcgggattc gagttgggga tcatgatcga
    17821 cacccaatcg ccctgcttca ctttcagcgc atccgccacg cctttgccga tgataatttg
    17881 ctgttcgccc gctttaaaat tgcgccacgc atccccctga acaaacgagg gtaatgcgct
    17941 cagacgctgt tcctgttgtg ggttaacgcc cttcacctgg attgcgcgca gattcgctcc
    18001 actttccacc agaccggtga aattgatata cggcgcagcg gcggcaatac ccggcacttt
    18061 ctgcacgtta tccagtgctt cctgccagtt agtccatggc tgatttaccg cttcgatttc
    18121 gccatgcggc accaccgcca gaatgcggtt attcagttcg cgttcaaagc cgttcatcgc
    18181 gcttaagccg acgatcaata ccgccacgcc gagggcaatg ccaatggtag aaatgacaga
    18241 gattagcgac accatgccgc cgcgtcgccg tccgcgacta aaacgcaggc caatcaataa
    18301 cgataaaggc atcgccatta ctccgccccc atcaggctca gttccgccgt cagacgtcca
    18361 tcacgcattt ccagttggcg gctcatacgt ttcgccagtt gcaggtcgtg agtgaccacc
    18421 aggaaagcgg tgccctgcaa gcgattcaac tccccaagca actggaagat gctgtcggcg
    18481 ttacgcgcat cgaggttacc ggtaggttca tccgccagta ccagacgcgg attattgacc
    18541 agcgcacggg caatcgctac acgctggcgt tcgccgccag aaagttcaga tgggcggtga
    18601 ttcgcacgat gctccagccc caccgctttt aacatctcca gtgcacggct gttgatttca
    18661 gcgggctttt tcttgccaat cagcagtggc atagccacgt tttccagggc agtaaaatcc
    18721 ggcagcaggt ggtgaaactg ataaataaag cccagcttct ggttgcgcag ttctgctttc
    18781 gccgctgaag acagtttgct catcggctga ccgttaaaga tcacatcgcc ggaggttggc
    18841 gtatccagcc cgcctaacag gtgcagcaag gtacttttac cggaaccaga gctaccgaca
    18901 attgccatca tttcaccttc gcccacgctg aaactgacat tgtgcagcac atcggtttgc
    18961 acactgcctt cctgatagcg tttgcacagg ttgtcgcatt gcaacaggat cttattcata
    19021 acgtaaggcc tcagcgggtt gtgtggcggc agcgcgccat gaagggtaaa gcgtagacag
    19081 cagcgcgata gccatcgcca ccagcgcaat aacaatgacc tgtaaaggtt cgatagccac
    19141 cggcagcgcc gcgccatcaa gcaggacgcc gattatcggc atcagattat ttaactggct
    19201 ggcaagcagt gcgccaagcg ccgctccgag gattgcaccg ataatcccgg cgctggcccc
    19261 ttgcaccata aagaccatca tgatttgtcg cggagttaag ccttgcgttt gcaggatcgc
    19321 gacttcgccc tgcttctcca tcaccatcag ccccagcgag gtaataatgt taaacgccgc
    19381 aacagcgaca atcaggctca gcagtaagcc catcatattt ttttccatgc gtactgcctg
    19441 gaacagctcg cctttacggt cacgccagtc ctgccatttg ctgccttcag gcagtttttg
    19501 ctgacttaaa gagtcaactt tcagcggctc atccagccac aaacgccagc cggtaatatt
    19561 ggctgccgga taacgcatca gacgcgaggc atcctcaata ttcaccagca tttcatagcc
    19621 atcgacttca ctgttggcgg cgaaagtgcc aatcacattg aacaggcgct gacttggaat
    19681 acgccccatc ggcgtgaact ggctggcaga tggcaccatc acgcggattt gatcaccgcg
    19741 attgacgccc aactgtgagg caagttgttc gccgaggatg acattatatt tccccggctc
    19801 gaggtcagtt tgtttcacat tgaccagata cggcgttagt ggatcttttt gcgccgggtc
    19861 gataccgagc atcaccccga ccgccacgct gcgcgcgctt tgcagtacca catcaccggt
    19921 agtaataggt gcgacgcgat taacgccgtc cagtttgact gccgtttccg ggagttgctg
    19981 cgggttaaga gagccatgct cagaagagag aattgcctgt ggcatcaggc caaggatgtt
    20041 gttttgcagc tcgcgctcaa agccgttcat cactgacaat actgtgacca gcgccatcac
    20101 cccgagggta atgccgatgg tagaaagcca ggagacgaaa cgaccgaagc gatccgctgc
    20161 acgcccacgc atgtaacgca ggccaataaa tagagcgaca ggttggtaca tgaaatccgt
    20221 ctggttgctg tagcaaagac acgagtatat aagcgaaagc aagatgggta aacgtctgaa
    20281 ctgtaagtga tcgtcagtgc taatctgaaa agcccccgta aatcaatcgg ctaaattgcc
    20341 atgttttatc atcaatcttg ccgcctgatc ttaatctgaa aaatcatcca atacagacag
    20401 atacccatta cacctcgggt cataaccagc aggatcaacc gcatgtaaat taaaaaagag
    20461 cgtgttgtcc tgatgaaaca aaaagagcta tggattaacc agatcaaagg gttatgtatt
    20521 tgtctggtgg tgatttatca ctcggtcatt accttttatc cgcatatgac cacttttcag
    20581 catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta ccttgccccc
    20641 tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta tatcgacagc
    20701 gtaccgtggg gaaattgtct cgataaacgg atctggaaca tcttctgggt gctggcgctt
    20761 tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc acctgagcgc
    20821 gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg
    20881 atgatcaccg ccagcaccag cttatggtat ctgtatgcgt taattgtcta tttcgtggta
    20941 tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg
    21001 gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct
    21061 tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg
    21121 ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg
    21181 tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat
    21241 cagtacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac
    21301 accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt
    21361 gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat
    21421 ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag
    21481 cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct
    21541 cgttaaacca tcgccccagc cggggcgatt caatttgcta aaccatgtcg attacggata
    21601 atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa
    21661 tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca
    21721 gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga
    21781 acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca
    21841 tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct
    21901 tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta
    21961 ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg
    22021 cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct
    22081 gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgccatg ttgaccaggt
    22141 gatggtgcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag
    22201 tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga
    22261 cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcgcacga
    22321 atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga
    22381 agtgaagcgc gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg
    22441 gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt
    22501 ccctgccaat accttgctcg tgaatactgg cgatctggaa accagtgccg aacgtttcca
    22561 ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc
    22621 accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg
    22681 agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca
    22741 gaaactgcca gacctggccg ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa
    22801 gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga
    22861 agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga
    22921 tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga
    22981 taaggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg
    23041 ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga
    23101 gctgcatatt ggtcagccgg tggtccatct ggagcacggc gtcggtcgct atgccggaat
    23161 gaccacgctg gaagctggcg gcattactgg cgagtacctg atgctcacct atgccaacga
    23221 cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc
    23281 ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa
    23341 agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc
    23401 cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag
    23461 cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttagcgacat
    23521 gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga
    23581 agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtag cggtgttggt
    23641 gcctaccacc cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg
    23701 gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct
    23761 tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag
    23821 tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt
    23881 gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc
    23941 aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat
    24001 cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct
    24061 ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta
    24121 caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgcctgaagc
    24181 acggattgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga
    24241 tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga
    24301 catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct
    24361 gcaccagtta cgcggtcgcg tcggacgttc acatcatcag gcatatgcat ggctgctgac
    24421 gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct
    24481 ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc gcggcgcggg
    24541 tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat
    24601 ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga
    24661 tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt
    24721 catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga
    24781 aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc
    24841 ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag
    24901 gaagctggaa ggtaatgaga aaggcggggt gatcgaattt gccgagaaga atcacgttaa
    24961 tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc
    25021 gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg
    25081 ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct
    25141 gcgcccccat ctgcaacatt tacaaattct ttgcacttcc ctgcactatc cgacactgtc
    25201 accatccata attcaggctt atctgtttat tacaataacc ttatatttat tatggatttt
    25261 tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc gccgctgccg
    25321 tggcgctggc gctaccggca aaagccaaca cctggccgct gccgccagcg agcagtcgtc
    25381 tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg
    25441 ccaaaaaata taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt
    25501 acgtaccgcg cgcgggcagc gtgttaacga tcccgttgca aaccctactt ccagacgcgc
    25561 cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta
    25621 aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac
    25681 cgacaatggt gaccacagtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa
    25741 acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg ccggctggac
    25801 cggataaccc aataggccat catgcgattc gtctggcggc ctatgacggc gtttatttgc
    25861 ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc
    25921 tgcgggatga cgatatcaaa acactcttta gccaggtcac cccaggcacc aaagtgaata
    25981 tcatcaacac tccggtaaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac
    26041 atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata
    26101 gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga
    26161 tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc
    26221 tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca
    26281 gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt
    26341 attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg
    26401 cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac
    26461 tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat
    26521 ggagcttaaa atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg
    26581 cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatcga tagatgtgat
    26641 ctggatcaca tacaagatat tttttataac aatcattaat taatttaaaa atctttaaat
    26701 ttcagttaaa tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta
    26761 tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc
    26821 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
    26881 tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct
    26941 cgtcgaagcg accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg
    27001 gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
    27061 gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc
    27121 caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac
    27181 cctctcccca gattcaggag atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga
    27241 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
    27301 tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
    27361 acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc ggaacgactt tgcgtttatg
    27421 gcccgaactg gtgaaataag tcgcaaaaaa agcccctcaa cctgagcgaa tgaggggcca
    27481 aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc gttactatct
    27541 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
    27601 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
    27661 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca
    27721 tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accaccaaac
    27781 tgatgcccta tcacgccacc agcaacagcg ccgagcaccg acccggtaat gcgattttca
    27841 tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta
    27901 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac
    27961 acgttcagac tggccactgc cgctacgccc aacgcagctg cgacaccaat cccgataccc
    28021 gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag
    28081 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatgac tggaaatact
    28141 taaggattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
    28201 ctaccgctcg taccatttgt ccagggcgga taaggcgttt atgccacatc cgccagtgta
    28261 cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat
    28321 cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat
    28381 atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat
    28441 caggctacca acggtggaga agttcgacag cgataccagc gaaccatgat ctttatactg
    28501 ataatttttc agcggcttac cgttcatctg cgccagaatg ttgttcattg cgcaagtcgc
    28561 catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga
    28621 cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgagtg gtttgcagcg tcggttccac
    28681 caccagctgg ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc
    28741 tttgatcccg gctgcccata ccatcagatc agcctcaata tattcgccat ctttagtgtg
    28801 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt
    28861 cgttagctcg ttgtgggccg cagcagagat acgtggcggt aatgcaggca aaatacgttc
    28921 tcccgcttct accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct
    28981 gtgcagttgc ttgaccgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc
    29041 gccgacaatc gcaatgttca ctttgccatt tgcgcccagg ttggcagagt atttcaggaa
    29101 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
    29161 gttctctttg acgcctggcg tattgaaatc gttagaggtg ctacccagcg ccatgaccag
    29221 ggtgtcatag gcgattttac gttccggaac cagcagttca cctttctcgt cgcgtagttc
    29281 tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca actggaactg
    29341 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
    29401 gccagtcgcc acttcgtgca gcagcggttt ccacaggtgg ctgtggttac gatcgaccag
    29461 cgtaattttg gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag
    29521 cccaccagca ccgccgccga caatcacaat ctttttcaat ggcgtagtca acgtgacccc
    29581 cttaaattta ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca
    29641 ggttacagca atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat
    29701 accaaaattg atgttaatca agttttgttg cttaaaagat aggcaacttt accgggtgag
    29761 atcaaaaaat agagtttatt gtttattatt tgagcaaatt cggaagagta atatagggtg
    29821 aagcgtatat cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt
    29881 gatgctcgac tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca
    29941 tcaggtaccg gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct
    30001 ttaatgcgct gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag
    30061 acaatctcgt aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca
    30121 ttacgcgaca aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac
    30181 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
    30241 gggaacaaat tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc
    30301 gcccagtatc cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc
    30361 aacattttgt ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg
    30421 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
    30481 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
    30541 gggttagtga cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct
    30601 gatcgctttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
    30661 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
    30721 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
    30781 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
    30841 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
    30901 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
    30961 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
    31021 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
    31081 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
    31141 cagggctttt tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc
    31201 gacatccagc actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
    31261 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa
    31321 ggattgtgct gccggtaagc gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc
    31381 ctgatctacc gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc
    31441 acgtaactgg gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg
    31501 atgcgccagt atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac
    31561 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
    31621 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctcctttga
    31681 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
    31741 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca
    31801 ccgccgccag ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta
    31861 accctgatgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
    31921 gtaaaggtcg tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccacag
    31981 tccgccgcgc cggatcgctt tgctgccagt acagttccag taagggcaac agcgttattc
    32041 gccagccaaa acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
    32101 ccggcaaata cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat
    32161 ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca
    32221 agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct
    32281 gatgttcaat gagaaaacta ccgccactaa gcccactatt aaacgtcgtg acgggatgaa
    32341 actgcgggaa aaagcgcgac agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg
    32401 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
    32461 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
    32521 cgggcaatgc ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac
    32581 tgttgcttag ccatcgacag ttgctgggcg gaaaccaggg taaatttccc gttattagcc
    32641 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
    32701 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
    32761 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
    32821 gccgttcgat cttcgtgctc aattgggcct agctgctgcg ggatcgtcgg caccgagggc
    32881 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
    32941 accggtgcag gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc
    33001 aaggcgtagc gactcatttt tgtcatcaag attcacccct ttacaaatat agataaagtc
    33061 tgactttgtg cgcccccaga aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg
    33121 gaatggtaac gctgcgtggc ctttccagag gatgcatctc cagccctctg gcgtcatacc
    33181 agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc gttataaagc gttgaggatg
    33241 ctgaaggttg aatatcagac gtcgaaagga cgggcttttc tgcactgatg cccgcagcca
    33301 ataaagatga ctccatcacc agcgattgct catcattcac cggaatttcc ggatgtgaac
    33361 gacagcccac cagcagtaac aacaccagag acaccagccc aaagcatcct tttctcatcg
    33421 ttacagacct ttatgcgcca gcattggtcc cagcggacgg ccacccaaca agtgcatatg
    33481 gatgtggtaa acctcttgtc cgccatggcg gttggtattc atgatcagac gatagccatc
    33541 ttcggcaata ccttcttgct cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc
    33601 ctgctcatgc tcagctgaca cgtcgttcac agtcggaatg aggatattcg gaatgatcag
    33661 aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat catcctggta
    33721 gacgatatcg gaggggatct cacgacgaat aattttgctg aatatagttt cttctgccac
    33781 gacattttcc tttttcaaat agccatgcta cgccgcgcca cactgcgagt atagagtatg
    33841 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
    33901 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
    33961 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttgcatttgc
    34021 aaacaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg
    34081 acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    34141 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    34201 tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    34261 gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg
    34321 cagatgactc aacgtgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa
    34381 gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt
    34441 caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg
    34501 gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat
    34561 atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg
    34621 ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg tgaatttaaa
    34681 ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa
    34741 agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac
    34801 gactcatggc tggaccgcta caatagtgaa aagaccttct tctcgggcat tgtcgatgct
    34861 gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat
    34921 agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat
    34981 cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt
    35041 atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct
    35101 gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt
    35161 atgctggttg ggccatacag taattatgga cctggctttg atcatgtcgg cggcaccggt
    35221 tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa
    35281 ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt
    35341 tacttcagtt catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat
    35401 ggcaatttcc cacaaaccga ctggtcacca cagagcctgg cgcaggacga taccacacat
    35461 atgaaatcgt tatatgctgc cactcgtgtc acccttgccg atccgctgca tctgatcctc
    35521 ggcgcacgtt ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac
    35581 accacgcctt acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc
    35641 tatacctcta ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca
    35701 atcaccggta acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc
    35761 accacgttag ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct
    35821 atccccggca gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg
    35881 gtggaatttg aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg
    35941 cgctatattg cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg
    36001 gttaaaatgt tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt
    36061 gttaactggc aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc
    36121 gagcaaggta gctacgcgct ggtggatctc ttcacccgct accaggtgac gaaaaacttc
    36181 tcgttacagg ggaacgtcaa taacctgttc gacaaaacct acgataccaa catggaaggt
    36241 tctatcgtct acggcgcacc gcgtaatttc agcattaccg gcacgtatca attctgaata
    36301 acacctgtaa aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag
    36361 tggttacgga tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc
    36421 gcctgaacac cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccagcc
    36481 tgatccactt tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg
    36541 ttagtaatgt tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta
    36601 cctgttgctt ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct
    36661 ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg
    36721 aacagccaca gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgag
    36781 aacgacgtac cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga
    36841 atcgcgtgga tgatgtacag gatcggcgca acgaacatga agaagaactc gatcggctcg
    36901 gtgataccgg tcaggaacga ggtcagcgcc gcggagatca taataccgcc cactttcgcg
    36961 cggttttctg gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt
    37021 ttgaacagga aaccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga
    37081 atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa
    37141 ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa
    37201 atgccaaacg caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca
    37261 gaaccaatcg gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca
    37321 gaaatgatcg gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata
    37381 cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg agataatccc tccgagtacg
    37441 ccagtatccg ccaagtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc
    37501 gcaaccacgg ccatggtttt aaccatgatg ccataggcaa caactgcggc cagcgcggat
    37561 acgccatcgt tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt
    37621 gcaaagacgg aaccgcctgc ttctgccata acatgcgata caacggcggg cagccagctg
    37681 aaattcgcgg aaccgacgcc cagcagaata cctgcgatag gtagtacgga taccggcagc
    37741 atcagcgatt taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt
    37801 gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc
    37861 cgtgtacagg gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca
    37921 atttgtttga cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac
    37981 aaaacatatg tcacaatatt tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac
    38041 tttactcgtt ttagccatta attacgagct ttaaaaaaac tcattcaacg gatggattga
    38101 aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt
    38161 tcttcaacgg caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca
    38221 ggttgattct cttttcctcg atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc
    38281 agtaatcgat ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag
    38341 gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca gtaatttacc cgccgtttct
    38401 ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga
    38461 attgccagcg tatcggcacg ggcgtcacgg gtatggacga taaccggctt gttcagttca
    38521 cgaccaatct ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg
    38581 taataataat ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg
    38641 cgtaaatctt ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat
    38701 acgacgttgt cacgttcgcc taccagatcc cgcatatgta aataacccgg taatgttgtg
    38761 gcgactgcca gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg
    38821 tccttatgca aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac
    38881 atgatgtctc tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc
    38941 aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg atgcctgtga cagacattaa
    39001 ctgttcacga atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg
    39061 gtttgccagt tcggcgacca ggcccggcac atcaacattg gtcacctgcg cagcgccatg
    39121 atggcgtttt agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg
    39181 ttcatgatta agggccgcca gcagcgaata ccagtcgccc gatggcacgc tatatgccaa
    39241 cgcctgacac aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc
    39301 gccaggcgaa ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac
    39361 ttcgcgtgaa agccaagtca cggcgtactg ttctggaggc ggcgcaaggt aatgtaaccg
    39421 acaacgacta cgtaatgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca
    39481 ggtttctgct ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag
    39541 taaggcagca tcggttaccc agacaacttt cgcaccacct aggcgtgcgt gctcattcag
    39601 cttttcggtg acctcacgta ccgcatcaat gcccagcgta tttttccctt tttcgggagc
    39661 cagggtgtag taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc
    39721 gcaacttttg tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa
    39781 agcatcatcg cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc
    39841 ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac catggatacc atctcatgcg
    39901 tccaactcct tcacccagtg ggtcacggta gtgcggattg catccatcac ggcctccagc
    39961 ggctgggtgg catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg
    40021 gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc
    40081 gcacgcgcgc gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtcgggg
    40141 cgaaaatccc cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt
    40201 cctccgccct gatacgcctg agtggagaga tcgtggcgat cgccaatcac ccaggtgccg
    40261 ttagccagcg caggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc
    40321 agaacttcgg ctttatcggt aatgacttca tcgcctaccg atttgatatc cagcaccagg
    40381 cttcttaact tttcggcaag ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg
    40441 atacccagtt gctcgagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct
    40501 tccagcccct caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata
    40561 atcctgcaca gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc
    40621 atcggccaca aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc
    40681 cgcccccggc gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc
    40741 tgtcggcgtt tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta
    40801 aatcacggtt gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga
    40861 ggcaacctga tcgcgttcac tggcaacggc ggtttctttt tcgataattg atgccatcgt
    40921 caccagctga tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc
    40981 gaccgctttc accattttct tgtgagctcg cttgagtaac gcgacatcgg tggtattggc
    41041 ggtatacatc aaggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc
    41101 aagtgcctgc gctacggtgg cgtacttatc gtcactcagc gtgtgcttga tatacggagc
    41161 ctcacgcaat tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagcgggaa
    41221 ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg cgcacggtca tctgcggtgt
    41281 aaagcggtaa gtcccggctt taaagtgaga aagatccggt tcgatacgca gcagccattg
    41341 aaaaacccgt gggcgattga tgatcttatc agcataaagc tgttcaccga gcgccagacg
    41401 tccggtccct ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc
    41461 aagatggcga accttccagg cgcccacacc agcggcgata cccagtacca ccagcaataa
    41521 caagataatc aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa
    41581 tattcatata acgttgctga cgaaaaggtg acatcgccac aggcacgtac gggcatcact
    41641 ggcattaacg cattacaaat aaccatctca tctgcctgca acgcctcctc cagcgaggct
    41701 tgcacttcga caagctgata agaggattgt gccagtaaac ggatacagaa ttgtcgcata
    41761 atgccgttaa cacctgcctg atccagtcgc ggcgtataaa ctacattgcc cttccgccag
    41821 aacaaattag ccgcacagca ttccgtaacc cacccttcgc agtcaaggac cagcgcctca
    41881 tcagcgtttg tctgctcaag atgagagcga atcaatactt gctcaagccg attaagatgt
    41941 ttaatacctg caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtcatc
    42001 ccttcgttac gcaaacggtc gtaatgtgca ggataagccg taacggagag aatccgcgtt
    42061 gctggtccgc tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact
    42121 ttcagcacac cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgaggc
    42181 cagaagtcac tggaaatcat caaccgctga caagcatcct gtagtcgctg gatatgcgcc
    42241 gataacaaac tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac
    42301 tgcgttgccc gatcgcttac tgccagcgat tcctgcttat aaccgttaat taagaacata
    42361 ccggctcctt atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa
    42421 taaaagctaa gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt
    42481 agatcttttt aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg
    42541 tgtattccat tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt
    42601 catccgggtt atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg
    42661 agtagataga ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg
    42721 agcttaccaa cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag
    42781 ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt
    42841 caatgcctgc atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg
    42901 gcggtgacgt catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa
    42961 ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat
    43021 cgcccagtac gaagccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat
    43081 cattgcgggt agataatgca cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg
    43141 ctttctctgc gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag
    43201 catggccaat gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac
    43261 gcaggccata catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga
    43321 agaatgggct gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca
    43381 gtccgaggcc gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg
    43441 ttatttcaag gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga
    43501 aggcatccat cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta
    43561 ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc
    43621 cactctgacc ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca
    43681 acatgcccag tccggtcaca actacacgac gcttagacac gtttgtcctc cagggaggga
    43741 aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat
    43801 gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt
    43861 ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc
    43921 aacggtgtca agagaatccg cgcctaggtc ttcaacgaaa gaagcattgt tggtaacttc
    43981 ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct
    44041 catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat
    44101 gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg
    44161 ccttcccccc caataacgca aatcattttg cactaattat tactcataac cacgcagact
    44221 gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac
    44281 atgcaaagtt tcccccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt
    44341 ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg
    44401 ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt
    44461 tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc
    44521 cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc
    44581 gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag
    44641 acggaaaaca gatgaaaggt tagtttcgat aatatcgttc cactcttcat ctttcattcg
    44701 cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa
    44761 ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat
    44821 cagacctttg ccgttggcac ctaaataatc actgatcgcc tgagcgccat tttcactggt
    44881 cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat
    44941 tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt
    45001 ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt
    45061 gtcgacaatg cgtttcgtca ggccagtaag caccttgccc gggccgactt catagagatg
    45121 ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa
    45181 ctgacgtacc agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt
    45241 attcacaaca gaaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt
    45301 gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagcgc
    45361 gcgttttgcg ccagcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc
    45421 ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc
    45481 agcttcttca cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc
    45541 gcccgtgcct tccggtacgg cttcttgcat gaacttaccg cgcatctcaa ccagacgcac
    45601 cgcatcagcg aaatcaatca cgccagcgca aaccagcgcg gagtattccc ccaggctgtg
    45661 accggccatc attgccggtg ctttaccgcc ctgctgctgc catacgcgat acagcgcaac
    45721 agatgcagtc aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc
    45781 ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc
    45841 ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg
    45901 tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag
    45961 cgcggagccc caagtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt
    46021 aatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt
    46081 accgtggcga tccagcgtca ccacgacatt gtccatcgac ataccgagtt ttttcgccgt
    46141 tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc
    46201 aagattattc gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt
    46261 gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc
    46321 gtttggcagc gtcagcaatt caccataact accgtcggca tgcagatggg tggaaatgat
    46381 tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat
    46441 agtcccacga tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc
    46501 atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc
    46561 tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat
    46621 ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc
    46681 aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat
    46741 ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac
    46801 aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc
    46861 gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg
    46921 ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc
    46981 cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg
    47041 caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa
    47101 aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac
    47161 aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta
    47221 gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca
    47281 gcagagaaag gaacatcctg acaacacctt ccatcgtctt taacgtgaca tttcctgtaa
    47341 agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc
    47401 cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcga
    47461 gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca
    47521 ccacctcttc agccagaact gagcccataa tggcaaattg caccaacatt gtgctgtcac
    47581 aatcgacgtt ggcccctaaa tcgaggacca ccgttttgcc cttttgctga tgtggtaata
    47641 ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc
    47701 ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca
    47761 gctccagggc catacgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg
    47821 catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt
    47881 cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt
    47941 gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc
    48001 ctcccatgac atctaacgcc agggttagac gtttcaaggt atcgtcgccg cccggtttgg
    48061 tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta
    48121 cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg
    48181 tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga
    48241 acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac
    48301 tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct
    48361 ggctaatacg gcaaatgggt ttggcttttg cacttcttca ggcagttcac caaagaccat
    48421 gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag
    48481 gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat
    48541 cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca
    48601 atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac
    48661 cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat
    48721 ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc
    48781 aggggtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt
    48841 caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc
    48901 aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact
    48961 gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt
    49021 aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta
    49081 gcctccacct cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt
    49141 gcagcaccag aggtcgacga aaccccacgc agcgacgaat caccgcgaca gttggtgctt
    49201 cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt
    49261 ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa
    49321 aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg
    49381 gcgttgttta attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat
    49441 ttccgtcacc taagcgaggc ggagattgat aattacgtgc gtaaggagca tcccctgcac
    49501 tgcgcgggta gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagaggga
    49561 cgtgacccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg
    49621 gaagggaaaa acccgctgat gggatagttg tttgcgccgg atgcggttac gtcgcatccg
    49681 gcaatcggtg caggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat
    49741 caggcttata tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc
    49801 ttcatccatc ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa
    49861 cgctgcggcg tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc
    49921 aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat actgtgtatg
    49981 cacacggatc tgatgagtac gtccggttac cgggctacaa cgcaccaggg tggcaaatgc
    50041 atagcgttct tccactttaa agcgtgtttc cgacggtttg ccttcctgac tgacacgcac
    50101 gatacgttcg ccgctttgca gaatattttt caataacggc gcttgaacgc tcttcacatg
    50161 cgactgccac tgaccgcgca ccagcgccag gtaatctttt tgcattcctt tttcacgtaa
    50221 ttgctcatgc agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaagt
    50281 gtcccggtcg agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa
    50341 accttcgata acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt
    50401 attcagcacc aggatatgat catcttcata taagatgacg tccgccagcg ccgccacctt
    50461 ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtgggatacg
    50521 cacctcatca cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac
    50581 ttcaccttta cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg
    50641 caaaaagtta tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga
    50701 tggagtctct gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat
    50761 ttttctatgc ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga
    50821 tggttattaa accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat
    50881 ggtaaaagtc atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc
    50941 catccttgtt aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac
    51001 gcagcaatgg cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc
    51061 cttaccggta gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg
    51121 cgctgttttt ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat
    51181 aaccgtgagg ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt
    51241 tactttgccc gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa
    51301 tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc
    51361 tgtatgacct ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag
    51421 gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac
    51481 gtcacggttt cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg
    51541 ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga
    51601 tcgataaaga agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg
    51661 gtagctatct ggttctgatg ccaaataacc cgcgcgcagg tggcatttct cgccgtatcg
    51721 aaggcgatga ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca
    51781 tggggcttat cgtgcgcacc gctggcgtag gcaaatctgc tgaggcgctg caatgggatt
    51841 taagcttccg cctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc
    51901 cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc cttccgcgat tatttacgtc
    51961 aggatatcgg cgaaatcctt atcgataacc cgaaagtcct cgaactggca cgtcagcata
    52021 tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc
    52081 cgctgttcag ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc
    52141 gtctgccatc cggtggttcc attgttatcg acagcaccga agcgttaaca gccatcgaca
    52201 tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga aactgcgttt aacaccaacc
    52261 tcgaagccgc cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg
    52321 ttatcgactt catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc
    52381 gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc
    52441 tgctggaaat gtcccgccag cgcctgagcc catcactggg tgaatccagc catcacgtct
    52501 gcccgcgctg ctccggtacc ggtaccgtgc gtgacaacga atcgctgtcg ctctctattc
    52561 tgcgcttgat cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc
    52621 ctgtgccaat cgcttcttat ctgctgaatg aaaaacgttc tgcggtgaat gcgattgaaa
    52681 cgcgtcagga cggtgttcgc tgcgtgattg tgccaaacga tcagatggaa accccgcact
    52741 accacgtgtt gcgcgtgcgc aaaggggaag aaacgccgac cttaagctac atgctgccga
    52801 agctgcatga agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc
    52861 ctgaacaacc tgcactggca acctttgcca tgccggatgt gccgccagcg ccaaccccag
    52921 ctgaacctgc cgcgactgtc gtagccccag caccgaaagc tgcaacggca acaccagcag
    52981 ctcctgcaca acctgggctg ttaagccgct tcttcggcgc actgaaagcg ctgttcagcg
    53041 gtggtgaaga agccaaaccg accgagcaac caacaccgaa agcagaagcg aaaccggaac
    53101 gtcaacagga tcgtcgcaaa cctcgtcaga gcaaccgccg tgaccgtaat gagcgccgcg
    53161 acacccgtag tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc
    53221 gccaggcaca gcagcaaact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg
    53281 aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta
    53341 acgatgataa acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg
    53401 ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc
    53461 agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg
    53521 tggttgaaga aactgccgct gccgaaccaa ttgttcagga agctccagct ccacgcacag
    53581 aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga
    53641 acaatgctga taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc
    53701 acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt
    53761 cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga
    53821 tccgctatcc aattgtacgt ccgcaagatg tacaagttga agagcagcgc gaacaggaag
    53881 aagtacaagt gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaacctgttg
    53941 ttagcgcgcc agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg
    54001 aaccacaacc ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg
    54061 aacagccgca ggtgattacc gagtctgatg ttgccgtagc ccaggaagtc gcagaacacg
    54121 cagaaccggt ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg
    54181 ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg
    54241 aagtcgcagc agaagttgaa acggtagccg cggttgaacc agagatcacc gttgagcata
    54301 accacgctac cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc
    54361 gtcacagtga ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc
    54421 atacggcaac gcatcatgcc tctgccactc ctgcgcgtcc gcaacctgtt gagtaataat
    54481 tagctcaaag taatcaagcc ctggtaactg ccagggcttt tttatttcat gtttgaatca
    54541 acatattaaa attcatcaaa tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa
    54601 aatgcccgcc ccggcgatcc cagagcgggc agacaaaaca tacccagttt catgatatgt
    54661 tttaaagcga gcttatttgt tgagctggaa gagcgacaat ccctgcatat cggtaaatgc
    54721 tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag ttgcattcca
    54781 gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat cgctacctaa
    54841 ggaatccagc gactccagtt tgttcagctg cgtgcctaat tccgcgcgaa cggtaaggac
    54901 attattcagc gagtttttca gtccacggtt ggttttatcc aatgccacag cggcgatttc
    54961 tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcgc tatccagcat
    55021 ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt tactggtaat
    55081 actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag catcgacctg
    55141 ttgcttaata ctttctgttc cgccgacata atcaccgtta gcctcactaa acggcgcagt
    55201 ctctgtttta taaccggcaa aaatgtagcg cccattaccg tcagtggtgt tcgccagatt
    55261 cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcccggt catcgtcact
    55321 caaggtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag cagtggtgac
    55381 ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt
    55441 gtactggctg ttttgcgcct gtgcctggga gagaacaaca gcttgtgatg cagcaatggg
    55501 atcgtctgaa gggttaacga ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca
    55561 ttctgcctga gaattggtga tgccgcgcat gttttgctgg tacatcatct gggtactgaa
    55621 acgcatttta tctcccctta gcgaatgtta atcagagcat caaaaatcgt gtttgccgtc
    55681 tgcagaacct gcgcattcgc caggtaatac tgctgaaaac gttgcagatt tccgtactcc
    55741 tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt caccacatta
    55801 ccttgcgtgg tgctactggt tttcaacatc gcggttttat taccgatatc actcactaac
    55861 gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt gctttgcaga
    55921 tccagcaggg cctgaccgtt gcggttgtcg ctatcacccg catcttcttc gctcgccatc
    55981 gcgattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc acttactggt
    56041 ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc cagaccatca
    56101 aatgccactt taccgttggc atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg
    56161 gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag taccacggag
    56221 gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc
    56281 gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc cgttagcatc aaatccggct
    56341 ttgtgttggc tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag cgtattacgc
    56401 gtctggtcca gatcctgaga acggaatgtc agaatgccgc ccagcgaccc ggtattcagt
    56461 aatttctccg ggatctcaat attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga
    56521 gaagggtcgg cgctggaagg aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt
    56581 gagtaaccat tggcatcgtg atgttataag taccgccatc ctgaacgctg acttctacgc
    56641 caacaatctg gtttaattcg ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg
    56701 acgcccctgc ccccacgcct gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt
    56761 tagcgtaatt gttgatctga tcaacgctgg cacctatcgc gatattgacc tgtttgtcct
    56821 ggtcgcgcag atattgatcg gtggttttaa actgattcac caacccttct gatttcccaa
    56881 tcagcgcctg gcgcgccgcc gggtcttccg cgttactcac cagcgtttgc aggctggtga
    56941 agaaatcctg catctgtgtt gccagcgaag aggtactggt ggagagcata ttgtcgattt
    57001 tcgacatctg ctcatagcgg gcggtcaggc cgctactttg cgtctgcgca gcacgtaact
    57061 ggttggtaat aaacgtatca tactcacgct gcacaccaga aacgtagacg ccattgccaa
    57121 cccagccgcc agcgcccaac gtgctattgg cctgcgccat aatagtggtt tggcgggtat
    57181 atccggcaac gttatagctg gagatattat tacttgccgt atttaacgcc gcctgggccg
    57241 cgttcagtcc gctcatggcg ttattgatca agctggacat ggaggttcct tataagcctt
    57301 caattacaga gtattatcgg cagcgacccg ccggacttga gttattcaga acagattatc
    57361 aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc gatttcatct gctggatcat
    57421 gttggtgagt ttacgggcat agtgaggatc ggtagcatag cccgcgtcct gtaggacctg
    57481 cgccccctgt tccgcactcg cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa
    57541 cagcccaacg taatccgaca atgcttccag atacgagctg tagacccgaa acttcgcttt
    57601 tactttcttc gcttcgccgt tttcatattc agtcgtggtg atttcagtga cttgcccttt
    57661 ccagttgcca gaggctttga caccaaacag gttatagctt ggctcgccgt tttcgtggcg
    57721 gatttgccgt tgcccccaac cagattcaag cgccgcctga gcgaggatca aatgatgtgg
    57781 cacaccgctt tgctggcttg ccagttgggc gggcagcgag agttgcgcga ggaatgctct
    57841 actgtcaccc ggcagcgaat catcgtagtt acgtggtacg gccttttgca ccagctgcga
    57901 aagcgtctgg ttttgataac gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg
    57961 cgtggactcc tctggcaatg gttgttctgg cgtcatctgt ttaaccatca tctctgcaag
    58021 ccccagacct ttgcccgccg tcatctgttg ggcaatttgc tggtcataca tactggtata
    58081 cagacgagtg tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt
    58141 caacatcatc tgcacgaaca tcccttccac ctgacgggcc accggacgga tatttgccgc
    58201 cggatcttcg ctcgatttcg cctttagttc gttgagtgat tgcgcatccc aggccgcact
    58261 tgccagtagt ttgctgtcgc tgatcatcag atgatttcca gttttgcccg cagacatccc
    58321 gcactttgca ttgattgcaa aatagacatc agatccatcg gcgtagcgcc cagcgcattg
    58381 agcgcacgca ccacgttatt gaggctggcg ctggaacgta cgctttgcag cgaaccgccg
    58441 ctctggcgta aatcgatctg cgtttgtgga gtaacaacag tctgtccacc accaaacggt
    58501 gtatctggct ggctgacatt ggcctgacga ttaactgtta ctgagagatt tccctgcgct
    58561 accgcgcagc tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta
    58621 atcactactt tagcgtcctg cggggtgaca ttaacctgca tattctggat atcggcaagg
    58681 aaacggacct gggaactgtt gccacttggt acgcgcacct gaatagtccg cgcatctaac
    58741 gcggtggcgc tgccatatcc acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg
    58801 ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa ctggctgggc
    58861 aattcacgtt caataaccgc accattggtg atccgtccac cgttcagttg gttaacctga
    58921 acactgctac cgcccgcgga ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc
    58981 gcatacacct gactgtcaac gcccttaagc ggtgtcatca acaacgtacc tccacgcaag
    59041 cttttggcat tacccatgga agaaaccacc acatcgatgg tttgcccctg acgtccaaac
    59101 ggaggaagtg acgctgtcac cattaccgca gcgacgtttt ttagctgcat attggtgccc
    59161 gtcggaacgg taattcccag ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc
    59221 gtctgggttg tctggtcacc ggtgccatcc agccccacta ccaggccata gccaatcagt
    59281 gagttttgcc ttaccccctg aacactggtg agatcgcgaa tacgctcagc ctgagccgcc
    59341 gtcgtaacca gtagaagaat taatgcagag agaaatttaa tcaccacagc ctcacttaca
    59401 ttggcgacag gttaaggaag aaacgctgca accagcccat attttgcgct tcgttaatgt
    59461 agccattgcc tacgtattca atacgcgcat ccgccacctg agtagacggt acggtattgc
    59521 tgccgctgat agtgcgtgga ttaaccacgc ccgagaagcg aataaattcg gtaccctgat
    59581 taatggcaat ctgtttttca cccaccacat gcaggttgcc gttgaccagt acctggtcaa
    59641 ccgtcaccgt caacgtgccg ctaaaggtat tgctggcatt ggccccgccc tttccgttga
    59701 acgtgttacc accggaggct tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat
    59761 agcgcggcac agtatcaaag ccaaaattag ttttaccgtc acggctgaca ttcgcagagg
    59821 agcttttgct ggcgctgacg ttctcctgca acacgatggt cagcgtatcg ccaatattgc
    59881 gtggtcgacg atcttcaaac agcggttgat agccatagtt aatcggctga gcagactgga
    59941 aaatagaacc gttggcgacg ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct
    60001 gcaccagcgg cgtggagggt atccaggcgc agccggttag tgaaagcacc aacaagctgg
    60061 aaatggcata agtatgcgca gcgttttttt gcatggctat catcttcaaa aatcagtaaa
    60121 ccggtgaacc tgccaccggt tatgccttag agttgcgtca gtttttgcag catctgatcg
    60181 gtggtggaca ccgctttact gttgatttcg taagcgcgtt gcacctgaat catattgacc
    60241 agttcttccg ccacgttgac gttagacgtt tcaacatacc cttgatacag cagtcccgcg
    60301 ccgttcaggc ccggcgtgct ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg
    60361 ttttcgccaa tgctctccag tccggtatca ttcataaagg tggtgagatt gagctgccca
    60421 acctgaaccg gagctgcctg gccttgttgg gttacgctga ccacgccatc acgaccgatg
    60481 gtgatactta acacattcgc cggaatggtg atcgctggct gcacctgaaa accaccagcc
    60541 gtcaccagct gcccgttctg atccacctgg aaagagccgt cacgggtata ggctgatgag
    60601 ccatctggca acattacctg gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta
    60661 ttggtctgcg acagattgcc ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc
    60721 gtgccgattt gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg
    60781 cgaatggttt gataaagcag atcttcaaac accgcgcgct gacgcttaaa accgttagta
    60841 ctgacgtttg ccaggttgtt ggcaatgacg tccatattgg tttgctgggc gtcaaggccc
    60901 gttttggcga tccataatga actgatcata taatgtcctg tagtggattg ctgacaagca
    60961 gagcagaagc acgcgacagt gtgaatttcc cctcacccta accctctccc caaaggggcg
    61021 agggaatagt ccgcgcgctt ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca
    61081 gcagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt
    61141 caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc
    61201 cttccagaac ccccgacatc acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg
    61261 tggcctgggt ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc
    61321 ccgtggcttt catcagtttc agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg
    61381 gattgagcgc cgagattgtg ccatcggcag caatcgtgat ttccgcccct tccggcacag
    61441 caattggccc cgcctcgcct atcaccggat gcccctgaat tgtcagttgc ccggtgggat
    61501 caacctgaat gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca
    61561 gccatcctgc tgcaacgcaa cgtccagcgg gcacgaggtg taatccatct tgccgggcgt
    61621 catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc
    61681 caccggcact gcgcgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc
    61741 cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt
    61801 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    61861 tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    61921 cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg
    61981 tcagggtgcc aaagtttccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    62041 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    62101 gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccgaca accgtaccgt
    62161 catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca
    62221 caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcaga ctaaacgtgg
    62281 cgggttctgc accgttaatt gcgccggttg cgatattatt cgccatcgca ccatccacta
    62341 atgtgccatt agcattaaat tccagtgttg tcgctgtctt cgcaatgctg tttggatcac
    62401 tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaaa tagacgctca
    62461 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
    62521 ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga
    62581 tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat
    62641 tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
    62701 gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac
    62761 ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag
    62821 caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac
    62881 cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg
    62941 tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt
    63001 tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa
    63061 atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga
    63121 gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg
    63181 gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt
    63241 tcggcgcagt gctgccatca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg
    63301 tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg
    63361 taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc
    63421 cttcttcact gccggttccc gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga
    63481 tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg
    63541 tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct
    63601 cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca
    63661 gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac
    63721 tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta
    63781 ttgaccgagc gtaagggttt tcagcaccat gcttttcacc gtgttgagca cttcaacatt
    63841 ggcctgatag ctgcgtggcg ccgacatggt gttaaccatc tccccgacga catcaacgtt
    63901 cggcattttt acgtagccct ttgcatctgc cagcggatta cctggttcat aaaccagttt
    63961 gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg
    64021 tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt
    64081 cacgctatca gcatttgcca gattactggc cgccacgttc aggcgctggg actgggcagt
    64141 taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg
    64201 taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg
    64261 caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag
    64321 cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc
    64381 cgggatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg
    64441 ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt
    64501 atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc
    64561 ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc
    64621 aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa
    64681 atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa
    64741 atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt
    64801 cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt ttagcgccca
    64861 actcgtgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc
    64921 gccatgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa
    64981 tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg
    65041 aaattatgtg gttgccgcga tgcctattgt gcggggagga aagctggaag ctggcaatgt
    65101 caaactgaaa cgtggacggc tggataccct gccaccgcgt acggtgctgg atatcaatca
    65161 acttgttgat gccgttagcc tgcgcgatct atcccccgat caacctatcc agttaaccca
    65221 gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga
    65281 tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc
    65341 gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag atgggaatat
    65401 tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca
    65461 tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa
    65521 gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acagccgggc
    65581 ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact
    65641 gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat
    65701 tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc
    65761 gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga
    65821 tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta
    65881 tgaggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg
    65941 cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca aatagcgttg
    66001 atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc
    66061 aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt
    66121 tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaccccatc
    66181 gcggcagtaa aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg
    66241 cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca
    66301 gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc
    66361 atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc
    66421 accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg cggagtaaag
    66481 attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca
    66541 atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt catcaattgc
    66601 gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa
    66661 aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt
    66721 aacgcccgct gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa
    66781 acggttaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagtgc caacaggaaa
    66841 caaagacgca acccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa
    66901 cttttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggaggga
    66961 aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag
    67021 gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggc
    67081 cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc
    67141 agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc
    67201 gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg
    67261 aaaccaatca tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga gaagcggttc
    67321 cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag
    67381 gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc
    67441 gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc
    67501 gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc
    67561 ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag
    67621 atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct
    67681 gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg
    67741 gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct aatccggacc
    67801 cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg
    67861 gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt
    67921 gtcaacgata cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt
    67981 ttgcacgcat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagagtgct
    68041 ctgccaacca ggaatcggtt tattcactac accctgcccg cgctcctcac gccattcgcg
    68101 catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact
    68161 tccggcacgg cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc
    68221 ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt
    68281 gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa
    68341 ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc
    68401 ggtggcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac
    68461 catcaacgtc agttttttcc gcgccgccag ttccaccagc ctttcagcat cgcgcagatt
    68521 ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac
    68581 gtcaaagtgg ctggcggtgc tggagtgcac aaaaaccgca tcacaactgg cggcaaggct
    68641 ggataacgaa tcggcataag gaatgcgcca gctgtcacaa attggcaggg ctttggcgcg
    68701 cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca
    68761 cgctttttgc gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat
    68821 ctcccagatg ggccagcaac gaatcaagac gttgtttcag ttctgccact tcgatttcca
    68881 gggcttcgac gcgggtctgt aaatcaccgt caaccacgtt cgacatagcc gttaccgccg
    68941 gctgatcttc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt
    69001 cgcgggccag acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtcg
    69061 actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac
    69121 ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca
    69181 gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac
    69241 caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca
    69301 cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga
    69361 ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc
    69421 aaccaattac gcgagcttcc agggcagtaa gttgatattt catgagattc tccttagcgg
    69481 ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca
    69541 atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca
    69601 ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc
    69661 tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt
    69721 tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa
    69781 ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa
    69841 ccttgtttat gaaattcgtt aatcatccct agcctggcct gccagcctga tggataacag
    69901 tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa
    69961 tccgccagac gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt
    70021 aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta
    70081 acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc
    70141 aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt
    70201 ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag
    70261 aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac
    70321 ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc
    70381 ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt
    70441 attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt
    70501 gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga
    70561 ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg
    70621 ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt
    70681 ccacaacaac gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc
    70741 attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca
    70801 ggggccgttt tgtttgtgct atgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa
    70861 ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt
    70921 tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg
    70981 atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa atggatgtat
    71041 gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag
    71101 cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg
    71161 ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc
    71221 gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga
    71281 gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt
    71341 tatatcggtg gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg
    71401 tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt
    71461 cagaaacgcg ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga
    71521 ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt
    71581 tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc
    71641 gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa
    71701 aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc
    71761 gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc
    71821 attgaagagt ggctgcgcaa ggtcaatgac tacgccaaca aactgctgtt gccgcgtttt
    71881 gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag
    71941 aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacag tctgattaag
    72001 aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat
    72061 ggcgaacttt cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta
    72121 gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca
    72181 caaatcaatt tgttatcatc aatggcgatt taatcctgaa ccgccgcggc agcctgtatt
    72241 cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc
    72301 tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga acaagttttt
    72361 atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata
    72421 caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga
    72481 tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacttga ccagccaaat
    72541 tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc
    72601 cctgttcggt agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga
    72661 taaagaaaaa ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc
    72721 ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa
    72781 ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat
    72841 ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac
    72901 ggattaatga cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg
    72961 cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc
    73021 accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg
    73081 cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat
    73141 gcccaatctg gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat
    73201 tcttgacgcc gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga
    73261 ttcgctggat cctaatgagc tggagcgcgg atttaatgaa ggcgtgttca ccgctgcaaa
    73321 actttacccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat
    73381 catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt
    73441 gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga
    73501 acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga
    73561 tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca
    73621 tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt
    73681 acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt
    73741 taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag
    73801 ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt
    73861 tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt
    73921 ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc
    73981 tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgagg aaacggtacg
    74041 ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat tttgcctgta
    74101 taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa
    74161 cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc
    74221 agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt
    74281 tatcggttat tggcgcaaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa
    74341 cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg
    74401 ccgggtcgcc ccggcttttt tattttactt acgaattttg agtacaaaat ttctacactt
    74461 cctcaaaata attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca
    74521 tataaatctt aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgaataccct
    74581 tacttattgg tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg
    74641 tctagtaaca cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat
    74701 ccgctcgtag ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg
    74761 cactaccaga ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg
    74821 ttaaccactg atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt
    74881 gaatccggtg atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac
    74941 ccaaaaggca cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa
    75001 ttactctgct taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt
    75061 ggcagcggtt ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg
    75121 ctaatgaccg acgcccatat gccaccgcat caacacggca gccaccacgg cgatacgcga
    75181 ttaattcgcc atgcttatgg tgaaggcgaa aagtatgtcc cgttggtcct ccgcgcgcaa
    75241 acgctgtggg atgaactttc ccgccacaac gaagatgatc ccatttttgt acgctctggt
    75301 gtcattaacc tcggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa
    75361 cagtggcaac tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa
    75421 atacgcgtcc cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc
    75481 gaactggcga ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc
    75541 aactgcccgg tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggttgac
    75601 ggtgagtatc aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc
    75661 ccggagctgc ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc
    75721 tatagcgtga agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat
    75781 tatggttttc cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt
    75841 atccattcag cggatgaacg tgttccgttt gcggaagtgg ccagtgatgg gtcagaagcc
    75901 ttcccgttct tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc
    75961 acctatgata attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat
    76021 acactgctca ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa
    76081 atagctgccg attttgcgca agaccaaaaa agcgattttg atttgacgcc attcaggctt
    76141 tcccgcttcc aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt
    76201 taatgcgtcg gctgttgcac ta
//
LOCUS       DADKXM010000004        72414 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.004, whole genome shotgun sequence.
ACCESSION   DADKXM010000004 DADKXM010000000
VERSION     DADKXM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.004"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..319)
                     /locus_tag="JQR05_000236"
     CDS             complement(<1..319)
                     /locus_tag="JQR05_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY5322496.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIH"
     gene            complement(336..596)
                     /locus_tag="JQR05_000237"
     CDS             complement(336..596)
                     /locus_tag="JQR05_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY5322497.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(587..1857)
                     /locus_tag="JQR05_000238"
                     /pseudo
     CDS             complement(587..1857)
                     /locus_tag="JQR05_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1935..2300)
                     /gene="queD"
                     /locus_tag="JQR05_000239"
     CDS             complement(1935..2300)
                     /gene="queD"
                     /locus_tag="JQR05_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY5322498.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            2616..4415
                     /gene="cysJ"
                     /locus_tag="JQR05_000240"
     CDS             2616..4415
                     /gene="cysJ"
                     /locus_tag="JQR05_000240"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY5322499.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            4415..6127
                     /gene="cysI"
                     /locus_tag="JQR05_000241"
     CDS             4415..6127
                     /gene="cysI"
                     /locus_tag="JQR05_000241"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY5322500.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILTSLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            6201..6935
                     /gene="cysH"
                     /locus_tag="JQR05_000242"
     CDS             6201..6935
                     /gene="cysH"
                     /locus_tag="JQR05_000242"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY5322501.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            7199..7351
                     /locus_tag="JQR05_000243"
     CDS             7199..7351
                     /locus_tag="JQR05_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5322502.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            7545..8696
                     /locus_tag="JQR05_000244"
     CDS             7545..8696
                     /locus_tag="JQR05_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY5322503.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPRPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(8782..9819)
                     /gene="iap"
                     /locus_tag="JQR05_000245"
     CDS             complement(8782..9819)
                     /gene="iap"
                     /locus_tag="JQR05_000245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY5322504.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KPPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10072..10980
                     /gene="cysD"
                     /locus_tag="JQR05_000246"
     CDS             10072..10980
                     /gene="cysD"
                     /locus_tag="JQR05_000246"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY5322505.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10982..12409
                     /gene="cysN"
                     /locus_tag="JQR05_000247"
     CDS             10982..12409
                     /gene="cysN"
                     /locus_tag="JQR05_000247"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY5322506.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSLGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12409..13014
                     /gene="cysC"
                     /locus_tag="JQR05_000248"
     CDS             12409..13014
                     /gene="cysC"
                     /locus_tag="JQR05_000248"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY5322507.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13064..13387
                     /locus_tag="JQR05_000249"
     CDS             13064..13387
                     /locus_tag="JQR05_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY5322508.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13581..13892
                     /gene="ftsB"
                     /locus_tag="JQR05_000250"
     CDS             13581..13892
                     /gene="ftsB"
                     /locus_tag="JQR05_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY5322509.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            13911..14621
                     /gene="ispD"
                     /locus_tag="JQR05_000251"
     CDS             13911..14621
                     /gene="ispD"
                     /locus_tag="JQR05_000251"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY5322510.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSENSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14621..15100
                     /gene="ispF"
                     /locus_tag="JQR05_000252"
     CDS             14621..15100
                     /gene="ispF"
                     /locus_tag="JQR05_000252"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY5322511.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTFGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15097..16146
                     /gene="truD"
                     /locus_tag="JQR05_000253"
     CDS             15097..16146
                     /gene="truD"
                     /locus_tag="JQR05_000253"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY5322512.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16127..16888
                     /gene="surE"
                     /locus_tag="JQR05_000254"
     CDS             16127..16888
                     /gene="surE"
                     /locus_tag="JQR05_000254"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY5322513.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            16882..17508
                     /gene="pcm"
                     /locus_tag="JQR05_000255"
     CDS             16882..17508
                     /gene="pcm"
                     /locus_tag="JQR05_000255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY5322514.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17648..18787
                     /gene="nlpD"
                     /locus_tag="JQR05_000256"
     CDS             17648..18787
                     /gene="nlpD"
                     /locus_tag="JQR05_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY5322515.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            18850..19841
                     /gene="rpoS"
                     /locus_tag="JQR05_000257"
                     /pseudo
     CDS             18850..19841
                     /gene="rpoS"
                     /locus_tag="JQR05_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(19961..20368)
                     /locus_tag="JQR05_000258"
     CDS             complement(19961..20368)
                     /locus_tag="JQR05_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY5322516.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            20515..21108
                     /locus_tag="JQR05_000259"
     CDS             20515..21108
                     /locus_tag="JQR05_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY5322517.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21108..22535
                     /locus_tag="JQR05_000260"
     CDS             21108..22535
                     /locus_tag="JQR05_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY5322518.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            22546..22782
                     /locus_tag="JQR05_000261"
     CDS             22546..22782
                     /locus_tag="JQR05_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322519.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(22822..24186)
                     /locus_tag="JQR05_000262"
     CDS             complement(22822..24186)
                     /locus_tag="JQR05_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY5322520.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSCGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMVGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(24281..25051)
                     /locus_tag="JQR05_000263"
     CDS             complement(24281..25051)
                     /locus_tag="JQR05_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY5322521.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDA"
     gene            complement(25056..25694)
                     /locus_tag="JQR05_000264"
     CDS             complement(25056..25694)
                     /locus_tag="JQR05_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY5322522.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(25691..26953)
                     /locus_tag="JQR05_000265"
     CDS             complement(25691..26953)
                     /locus_tag="JQR05_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY5322523.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMHHHPVNPMTDRYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(26950..27722)
                     /locus_tag="JQR05_000266"
                     /pseudo
     CDS             complement(26950..27722)
                     /locus_tag="JQR05_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            27888..28685
                     /locus_tag="JQR05_000267"
     CDS             27888..28685
                     /locus_tag="JQR05_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5322524.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(28736..29392)
                     /gene="pphB"
                     /locus_tag="JQR05_000268"
     CDS             complement(28736..29392)
                     /gene="pphB"
                     /locus_tag="JQR05_000268"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY5322525.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRMQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(29498..32059)
                     /gene="mutS"
                     /locus_tag="JQR05_000269"
     CDS             complement(29498..32059)
                     /gene="mutS"
                     /locus_tag="JQR05_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY5322526.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNGPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32135..32314
                     /locus_tag="JQR05_000270"
     CDS             32135..32314
                     /locus_tag="JQR05_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322527.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            32346..32699
                     /locus_tag="JQR05_000271"
     CDS             32346..32699
                     /locus_tag="JQR05_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY5322528.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32736..34804)
                     /gene="flhA"
                     /locus_tag="JQR05_000272"
                     /pseudo
     CDS             complement(32736..34804)
                     /gene="flhA"
                     /locus_tag="JQR05_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(34878..35888)
                     /gene="hypE"
                     /locus_tag="JQR05_000273"
     CDS             complement(34878..35888)
                     /gene="hypE"
                     /locus_tag="JQR05_000273"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY5322529.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(35885..37006)
                     /gene="hypD"
                     /locus_tag="JQR05_000274"
     CDS             complement(35885..37006)
                     /gene="hypD"
                     /locus_tag="JQR05_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY5322530.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(37006..37278)
                     /gene="hypC"
                     /locus_tag="JQR05_000275"
     CDS             complement(37006..37278)
                     /gene="hypC"
                     /locus_tag="JQR05_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY5322531.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(37269..38141)
                     /gene="hypB"
                     /locus_tag="JQR05_000276"
     CDS             complement(37269..38141)
                     /gene="hypB"
                     /locus_tag="JQR05_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY5322532.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYRHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(38145..38495)
                     /gene="hypA"
                     /locus_tag="JQR05_000277"
     CDS             complement(38145..38495)
                     /gene="hypA"
                     /locus_tag="JQR05_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY5322533.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            38707..39168
                     /gene="hycA"
                     /locus_tag="JQR05_000278"
     CDS             38707..39168
                     /gene="hycA"
                     /locus_tag="JQR05_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY5322534.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            39293..39904
                     /locus_tag="JQR05_000279"
     CDS             39293..39904
                     /locus_tag="JQR05_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5322535.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            39901..41727
                     /gene="hycC"
                     /locus_tag="JQR05_000280"
     CDS             39901..41727
                     /gene="hycC"
                     /locus_tag="JQR05_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY5322536.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAIFKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            41730..42653
                     /gene="hycD"
                     /locus_tag="JQR05_000281"
     CDS             41730..42653
                     /gene="hycD"
                     /locus_tag="JQR05_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY5322537.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            42671..44380
                     /locus_tag="JQR05_000282"
     CDS             42671..44380
                     /locus_tag="JQR05_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY5322538.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRLA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            44390..44932
                     /gene="hycF"
                     /locus_tag="JQR05_000283"
                     /pseudo
     CDS             44390..44932
                     /gene="hycF"
                     /locus_tag="JQR05_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
     gene            44932..45699
                     /gene="hycG"
                     /locus_tag="JQR05_000284"
     CDS             44932..45699
                     /gene="hycG"
                     /locus_tag="JQR05_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY5322539.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            45696..46106
                     /locus_tag="JQR05_000285"
     CDS             45696..46106
                     /locus_tag="JQR05_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY5322540.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46099..46569
                     /gene="hycI"
                     /locus_tag="JQR05_000286"
     CDS             46099..46569
                     /gene="hycI"
                     /locus_tag="JQR05_000286"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY5322541.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            46612..47363
                     /locus_tag="JQR05_000287"
                     /pseudo
     CDS             46612..47363
                     /locus_tag="JQR05_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47536..47847
                     /locus_tag="JQR05_000288"
     CDS             47536..47847
                     /locus_tag="JQR05_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY5322542.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            47844..48263
                     /locus_tag="JQR05_000289"
     CDS             47844..48263
                     /locus_tag="JQR05_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5322543.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(48378..49802)
                     /locus_tag="JQR05_000290"
     CDS             complement(48378..49802)
                     /locus_tag="JQR05_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY5322544.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(49811..51268)
                     /gene="ascF"
                     /locus_tag="JQR05_000291"
     CDS             complement(49811..51268)
                     /gene="ascF"
                     /locus_tag="JQR05_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY5322545.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            51525..52538
                     /locus_tag="JQR05_000292"
     CDS             51525..52538
                     /locus_tag="JQR05_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5322546.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            52687..53214
                     /gene="hydN"
                     /locus_tag="JQR05_000293"
     CDS             52687..53214
                     /gene="hydN"
                     /locus_tag="JQR05_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY5322547.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            53367..55619
                     /gene="hypF"
                     /locus_tag="JQR05_000294"
     CDS             53367..55619
                     /gene="hypF"
                     /locus_tag="JQR05_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY5322548.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPLLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWLAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(55747..56880)
                     /gene="norW"
                     /locus_tag="JQR05_000295"
     CDS             complement(55747..56880)
                     /gene="norW"
                     /locus_tag="JQR05_000295"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY5322549.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRTDDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTGMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(56877..58316)
                     /gene="norV"
                     /locus_tag="JQR05_000296"
     CDS             complement(56877..58316)
                     /gene="norV"
                     /locus_tag="JQR05_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY5322550.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            58503..60017
                     /gene="norR"
                     /locus_tag="JQR05_000297"
     CDS             58503..60017
                     /gene="norR"
                     /locus_tag="JQR05_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY5322551.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            complement(60014..60979)
                     /gene="gutQ"
                     /locus_tag="JQR05_000298"
     CDS             complement(60014..60979)
                     /gene="gutQ"
                     /locus_tag="JQR05_000298"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY5322552.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(60972..61745)
                     /gene="srlR"
                     /locus_tag="JQR05_000299"
                     /gene_synonym="gutR"
     CDS             complement(60972..61745)
                     /gene="srlR"
                     /locus_tag="JQR05_000299"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY5322553.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(61812..62171)
                     /gene="gutM"
                     /locus_tag="JQR05_000300"
                     /pseudo
     CDS             complement(61812..62171)
                     /gene="gutM"
                     /locus_tag="JQR05_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            complement(62276..63055)
                     /gene="srlD"
                     /locus_tag="JQR05_000301"
     CDS             complement(62276..63055)
                     /gene="srlD"
                     /locus_tag="JQR05_000301"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY5322554.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(63059..63430)
                     /gene="srlB"
                     /locus_tag="JQR05_000302"
                     /gene_synonym="gutB"
     CDS             complement(63059..63430)
                     /gene="srlB"
                     /locus_tag="JQR05_000302"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY5322555.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(63441..64400)
                     /gene="srlE"
                     /locus_tag="JQR05_000303"
                     /gene_synonym="gutE"
     CDS             complement(63441..64400)
                     /gene="srlE"
                     /locus_tag="JQR05_000303"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY5322556.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(64397..64960)
                     /gene="srlA"
                     /locus_tag="JQR05_000304"
                     /gene_synonym="gutA"
     CDS             complement(64397..64960)
                     /gene="srlA"
                     /locus_tag="JQR05_000304"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY5322557.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            65216..66301
                     /gene="mltB"
                     /locus_tag="JQR05_000305"
     CDS             65216..66301
                     /gene="mltB"
                     /locus_tag="JQR05_000305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY5322558.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            66446..66943
                     /gene="pncC"
                     /locus_tag="JQR05_000306"
     CDS             66446..66943
                     /gene="pncC"
                     /locus_tag="JQR05_000306"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY5322559.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            67023..68084
                     /gene="recA"
                     /locus_tag="JQR05_000307"
     CDS             67023..68084
                     /gene="recA"
                     /locus_tag="JQR05_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY5322560.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            68327..68827
                     /gene="recX"
                     /locus_tag="JQR05_000308"
     CDS             68327..68827
                     /gene="recX"
                     /locus_tag="JQR05_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY5322561.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            68955..71585
                     /gene="alaS"
                     /locus_tag="JQR05_000309"
     CDS             68955..71585
                     /gene="alaS"
                     /locus_tag="JQR05_000309"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY5322562.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKQEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLGRTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVTEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            71820..72005
                     /gene="csrA"
                     /locus_tag="JQR05_000310"
     CDS             71820..72005
                     /gene="csrA"
                     /locus_tag="JQR05_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY5322563.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            72321..72413
                     /locus_tag="JQR05_000311"
     tRNA            72321..72413
                     /locus_tag="JQR05_000311"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72355..72357,aa:Ser,seq:gct)
BASE COUNT        17026 a        18690 c        19675 g        17023 t
ORIGIN      
        1 gatgaataca gaaaaaaact tccgctcttg ccgcttttag catcgatgct ttggtgctga
       61 taattccatc cgcctcggtc accagtttca aaaactgaat caccacttct ttatttgacg
      121 cgccttccag caaatcaaca tgaataaaag catatttccc ggcgttctta atttttttaa
      181 cgatattact gatggtgcag atattgccgt acaacacgga aataaactgg cattcagaat
      241 cgatggccag ttgcaggctg gcattatctt taacggcagc aatcaccgga ttctggcgga
      301 gcaggtgtaa gaggggcata aattatcctt attatttatc caaagcggta ggtgatgccg
      361 aatccggaag gcggatagcg ccactgttgt agtgtggatt cgtcgcacag caaacggcag
      421 gtgccacact ccaggcagcc gcgatagtca atacgtaagt caccttcctg tgtgagagaa
      481 aaaagaccta ccggacaggc gttaatcaac cgttgtacca tctgacgctc aacggagtca
      541 gccggaacaa tgtgcggcgc atcagcaacg cgccagagat tacgggctac agacatcgca
      601 gactcctgat aacatcgccc gccagatgcc acaggccatg acgacgtaaa tgacgccaga
      661 ataactggcg cagcggtgga acaggtttat caccctgatc ccataaatcg cgggaaatat
      721 cctgcattaa cgcaggccac gcccgatacc agccaggggt tgcaaaagcg ccggaacatg
      781 ctgataacgt tgtagaacat cccatagcag gctgcgctct acgttgtgat gataaagcgc
      841 aaacagattt tgcggctcgc ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg
      901 tgcgccagtc agcgccatat ccatgccgcg cacggaaatt ccggtattga cgcaactgcg
      961 caacgcatcg cccaccagca gccagccgtt accggcgtat tgcaccggca tactgtgcaa
     1021 gccaccttct ggcaccagat gcgcaccata ctccagtgat tccgtgtttt tgataagcgg
     1081 gcgcactgcc ggatgcgctt taaagcgagt cagcagctcg cttgccggaa cacgactttg
     1141 cgtaagggaa gagagcgggc aaacaatccc taacgagagc gtttgttgat tagtataaag
     1201 aaatgcgccg ccgggtaagt catcacagat tccgccgctg aacagcaacg ctgcgccttc
     1261 gttattctcc agatgaaaac gttcttcaat agcggatgtt tccagcgaca gcacttcttt
     1321 gatccccaac gccatcgctt cgccagcagg acgagtcact aacccgtgac gttcagccag
     1381 gacgctgttg gcaccttctg ccagcaccac ataacgggcg cggagaatat cgtcaccaca
     1441 aataacgcca cagactctgc cgttttcttc atacagtgca tccaccgtcg ctccggggat
     1501 gcattcgaca ccttcttttt cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa
     1561 tacactccag gattcaccgc cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt
     1621 taacagggaa aggctttcgt gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg
     1681 cgggaggagt tccgcgagtg catgggtata taaccgcccg ccggaaagat ttttgctgcc
     1741 ggggatttca gcgcgttcga gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc
     1801 gcaagcggtg cctgcaatac cggcaccaat aataataatg tcgcagtcgt cttccatgtc
     1861 atgccccaga tatccagaac acaacaccct aacatagcgt tactaaaggg aaattgaccg
     1921 ccgacacact cttatcattc gccgcgatag atacaccctg cggtgcaggt ttcttttacc
     1981 atcaccgcac ttaacagcgg cacaacgggt ttaacctgat cccaaatcca ttttgctaaa
     2041 acttcgctgg ttgggttttc cagacctgga atatcattga gataatggtg atcgaggcgc
     2101 tcataagttg gtttaaacgc cgctttcagt tcagcgaaat cgataatcca gcccgtatgc
     2161 ggatcgactt ccccggtaat ttccagtcgc accataaagg aatgcccgtg caggcgacca
     2221 catttatgcc cttccgggac atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta
     2281 aataacgtgg tggacatcat aatttctcta caggacgata aaaccgccgt agagtaccgg
     2341 aaaggctgtt ttttcgcatt atctaaaacg actttgttgc gcaaaatcgc tgatttatct
     2401 taatgattgg ctaaattcat ttgtttttca ttaggttggt taatctattt tgttgttaaa
     2461 gactattgct aaaacaggtt agtcgatttg gttattagtt atcgctatcc cgtctttaat
     2521 ccacaccgtt caccccgtta accttacctt ctcttctgtt ttatgggcgc tgacagggcg
     2581 cagaaacagc tttgcttact ggaacataac gacgcatgac gacacaggtc ccaccttccg
     2641 cgttgcttcc gttgaacccg gagcaactgg cacgccttca ggcggccacg accgatttaa
     2701 cgcctaccca gcttgcctgg gtttctggct atttctgggg cgtgctcaac cagcagcctg
     2761 ttgcgcttgc agtgacgcca gcgcccgccg cagaaatgcc gggtataacg attatctctg
     2821 cttcgcaaac cggcaatgcg cgccgggttg ctgaagcatt acgcgatgat ttattagcag
     2881 caaaactgaa cgttaagctg gtgaacgcgg gcgactataa attcaaacaa atcgccagcg
     2941 aaaaactgct catcgtagtg acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg
     3001 tcgcgctgca taagttcctg ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg
     3061 ccgtgtttag cctcggcgat agctcttatg aatttttctg ccagtccggg aaagattttg
     3121 acagcaagct ggcggaactg ggtggtgaac gcctgctcga ccgtgtcgat gctgatgttg
     3181 aataccaggc tgctgccagc gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg
     3241 cgcctgtcgc ggcaccttcg caatccgtcg ctactggcgc ggtaaatgaa atccacacca
     3301 gcccgtacag caaagacgcg ccgctggtgg ctagcctctc tgttaaccag aaaattactg
     3361 ggcgtaactc tgaaaaagac gttcgccata tcgaaattga cttaggcgac tcgggcctgc
     3421 gttaccagcc gggtgacgcg ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag
     3481 aacttgtcga actgctgtgg ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt
     3541 tgcctctgaa cgaagcgcta cagtggcact tcgaactgac cgtcaacacc gccaacattg
     3601 ttgagaatta cgccacgctt acccgcagtg aaacactgct gccgctggtg ggcgataaag
     3661 cgaagttaca gcattacgcc gcgacgacgc cgattgttga catggtgcgt ttctccccgg
     3721 cacagcttga tgccgaagcg ctaattaatc tgctgcgccc gctgaccccg cgtctctatt
     3781 ccattgcctc ctcgcaggcg gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc
     3841 gttacgacgt ggaaggccgc gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg
     3901 tggaagaaga gggcgaagtc cgcgtattta tcgaacataa cgataacttc cgcctgcccg
     3961 ctaacctgga aaccccggtg attatgattg gcccaggcac cggtattgcg ccgttccgcg
     4021 cctttatgca gcaacgcgcc gctgacgaag cgccaggtaa aaactggctg ttctttggca
     4081 atccgcattt tacggaagac ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg
     4141 gtgtgctgac gcggatcgat ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac
     4201 aagataaact gcgcgaacag ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca
     4261 tttatgtctg cggcgacgct aatcgcatgg cgaaagacgt tgagcaggca cttctggaag
     4321 tgattgccga atttggtggc atggacaccg aagcggcgga tgaattttta agtgagctgc
     4381 gcgtagagcg tcgttatcag cgagatgtct actaatgagc gaaaaacatc cagggccttt
     4441 agtggtcgaa ggaaaactga cagacgccga gcgcatgaag cttgaaagca actacctgcg
     4501 cggcaccatt gcggaagatt taaacgacgg tctgaccggc ggctttaagg gcgataactt
     4561 cctgctgatc cgcttccacg gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg
     4621 tgctgaacag aagctggagc cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg
     4681 agttatcacc accaaacagt ggcaggcgat cgataaattt gccggtgaaa acaccatcta
     4741 tggcagcatt cgcctgacca accgccagac gtttcagttc cacggcattc tgaaaaagaa
     4801 cgtcaaaccg gtgcaccaga tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa
     4861 cgacatgaac cgtaacgtac tctgcacctc gaacccttac gagtcgcagc tgcacgcaga
     4921 agcgtacgag tgggcgaaga agatttctga acatctgctg ccgcgtaccc gcgcgtatgc
     4981 ggagatctgg ctcgatcagg aaaaagtcgc caccaccgat gaagaaccga tccttggtca
     5041 gacctacctg ccgcgtaagt tcaaaaccac ggtagtgatc ccgccgcaga acgatattga
     5101 tctgcacgcc aacgacatga acttcgtggc gatcgcggaa aacggcaagc tggtgggctt
     5161 caacctgctg gtgggcggtg ggctttctat cgaacacggt aacaagaaaa cctacgcccg
     5221 cacggcgagt gagtttggct atctgccgct agagcatacg ctagcggtgg cggaagccgt
     5281 cgtgacaact cagcgtgact ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata
     5341 cacactggag cgcgtggggg ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat
     5401 caaatttgaa ccgatccgcc cgtatgaatt tacagggcgc ggcgatcgta ttggctgggt
     5461 taagggcatt gatgataact ggcacctgac gctgtttatc gaaaatggtc gcatccttga
     5521 ttatccgggg cgtccgctga aaactggcct gctggagatc gcgaagatcc acaaaggtga
     5581 tttccgcatt acggcgaacc agaatctgat catcgccggt gtgccggaaa gcgagaaagc
     5641 gaagatcgag aaaattgcca aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga
     5701 aaactcgatg gcctgcgtgt cattcccgac ttgcccgctg gcgatggcgg aagcggagcg
     5761 tttcctgccg tcttttatcg acaacatcga taatttaatg gcgaaacatg gtgtcagcga
     5821 tgagcatatc gtgatgcgtg taacaggctg cccgaacggt tgtggtcgcg cgatgctggc
     5881 ggaagtgggc ctggtgggta aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg
     5941 cattgggaca cgtatcccac ggatgtataa agaaaacatc accgagccgg aaatcctgac
     6001 gtcgcttgat gaactgatag ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg
     6061 cgactttacg gtgcgtgcgg gcatcattcg cccggtgctc gatccggcgc gcgatttgtg
     6121 ggattaacca tcagcccggt cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca
     6181 aggcaaacag tgaggaatct atgtccaaac tcgatctaaa cgccctgaac gaactgccga
     6241 aggtagatcg cattctggcg ctggcagaaa ctaacgccga actggaaaaa ctggacgctg
     6301 aaggccgcgt tgcctgggcg ctggataatc tgcccggtga atatgtactt tcttccagct
     6361 ttggcattca ggcggcggtg agcctgcatc tggtgaatca gattcacccg gatattccgg
     6421 tgatcctcac cgacaccggc tatctgttcc cggaaaccta ccgctttatt gacgagttaa
     6481 cggacaaact caagctcaac ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg
     6541 aagcacgcta cggcaaactg tgggaacagg gcgttgaagg cattgaaaag tacaatgaca
     6601 tcaacaaagt cgaaccgatg aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg
     6661 ctggcctgcg gcgtgaacaa tccggcagcc gtgccaattt accggtgctg gcaattcagc
     6721 gtggcgtatt taaagtgctg ccgattatcg actgggataa ccgaactatt tatcagtacc
     6781 tgcaaaaaca tggcctgaaa tatcacccat tatgggacga aggatattta tcggtgggcg
     6841 atacccatac aacccgtaaa tgggaacccg gcatggcgga agaagaaacg cgtttctttg
     6901 gcttaaaaag ggaatgtgga ttacacgaag ggtaaatatt gccggataaa ttaaacgcct
     6961 ctgtcagaaa tgatgggggc gtttttattt tgcgagcagc attccggcat ttaaaggtga
     7021 acatcgcagg gaaaattaac aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt
     7081 taagcattcg cgaggttcca gatggacaaa agccccaggc gatatttcta tcaacctgag
     7141 gctcgcgttc gaacgtagca attcggatgt tagtctcttc tttgcaggga ggcaagacat
     7201 gctgacaaaa tatgcccttg tggcaatcat cgtactgtgt tgtacagtac tgggattcac
     7261 gttgatggta ggtgactcgt tatgtgagtt gagtatcaga gaacgtggta tggagtttaa
     7321 ggcagttctc gcttacgaat cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac
     7381 tgaattgtga tggtttggtc tcctgcaccc ttttttaaaa tttatatttt ttatgtgaat
     7441 atatagtctc tatttattat tatgaatttc aaatgaaaat cattaattta ttcataatgt
     7501 aaatgtttta ttggctgata tatatcacag ataattttta aaatatgcgt aaatatcctt
     7561 taagtttact gaaggataaa aatattgtga ctttctttga tttctgggga aaaacccgac
     7621 gtggcgagaa agagggtagc gacgactatc accttctttg ctggcatttg ctggatgtgg
     7681 ccgcaatggg ctatttaatg gttaaaagaa attgcttcgg gctggctgat tattttcgtc
     7741 aattagggat ttctgacaag gaacaggcag ctcaattttt cgcctggttg ctgtgctggc
     7801 acgatattgg aaaatttgcc cgctcttttc aacaacttta cctgccccct gaattcaaga
     7861 ttcaggaagg tgccagaaaa aattacgaaa aaatctctca ttcaacgctg ggctactggt
     7921 tgtggaatca ttatttaagt gaatgtcagg agttacttcc ttcatcttcg ctctctccgc
     7981 gcaaacttag gcgagtgata gaaatgtgga tgcccgtgac gacagggcat catggtcggc
     8041 cccctgatcg tatggatgaa ttggataatt ttctgcctga agacaaagct gcagcacgag
     8101 attttctcct tgaaatcaag ccactgtttc cgcgaataga gatccccaca ttctgggatg
     8161 atgacgaggg cgttgaactt ataaaacaac tttcctggta tatctctgca accgtcgtac
     8221 ttgcagactg gacagggtcg tcaacgcgat tttttccacg cgtcgcacag gcaatggata
     8281 ttgaagatta ctggcaaaag gctttagttc aggctcaaaa cgcattaacc gtctttccgc
     8341 cacaagcaga aaccgcgcct ttcaccggaa ttaatacgct gtttcctttt attgagaata
     8401 cgacaccgtt acagcaaaag gtgctggatc tggatatcaa tcagccaaga ccacagttgt
     8461 ttattctgga agatgtgacc ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca
     8521 ggttgatggc tgcgggtaac gctcaggggc tgttttttgg cctgccaaca atggcaacgg
     8581 ccaatgccat gtacgatcga ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt
     8641 tccccgctcc agcggagata aaccggcgca atacggtgcg gttttcccgg aagtaaaccg
     8701 gacgcactgg atgcgatgat gggtatcact tagaattccc cgcccctgcg gtagaactcc
     8761 cagctcccat tttcaaaccc atcaagacgc cttcgccagc tccttcacca acggtagcat
     8821 tatccgcata acgtcacggc agcgacgttc tattcttcca ggaagtgcct tatcaatatg
     8881 ttgttgatta tccagtctta cgtcatgcca gctatttccc gccgggaagg caggtgtttt
     8941 tgcgcgttgc tgataaccat ccttatttcc aagattccag ttagtcgctt ccaccgaaag
     9001 tacagcaatg cccgctttgt cgaatatttc tgcgtcatta caacacccag tgcctttcgg
     9061 ataattttta ttcaaacccg gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc
     9121 cagcgccctg tcgcgcgtta atttccttac tgcttcaggg ggttttacac cgctgttgaa
     9181 atacaattta tcgccaacaa ttaagttatc gagattaatc accagcagcg tatttttctt
     9241 ttcggtgtca ctcatccgct tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc
     9301 gctggtcgcc acaaatcgaa taccatactc ggtaggcgta tttttcaggc gttctgccag
     9361 ttccagcatg acacctaaac ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc
     9421 gagattggca tctgcgtcag catcgctcag cggggcgtaa gtatccagat gcgccataat
     9481 gatgatctgc tgcggcgctt tgccttcatg agcggcaatc accgtacttc ctgtcacgtt
     9541 atgccagttc ttgcgattat cgcgggcggt ataaatatac cgactattaa atgtccgaat
     9601 atcactgcga taacccattt gctgaaactg ttggcgaata taatcggcag ataacatttc
     9661 tgctggagtt ccggtcatgc gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt
     9721 agcaaaatcg ccaggtttag gtgacgaggc gtgtacgggg agaataaagc atacgccgag
     9781 cgccagggca gcggtacggt ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg
     9841 caaatttttt taccgccata gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc
     9901 ctaaacgccc gaaatccggt gccttaagca ctttttgata ttagctttgc caaatcgtta
     9961 ttccgttaag gaactactca ttctaattgg taatttcatt cgttctctta cgctccctat
    10021 agtcagaaac atctgatggc aagaaaatag cggtattgca aaggaacggt tatggatcaa
    10081 aaacgactta ctcacctgcg gcaactggag gcggaaagca tccacattat tcgcgaggtg
    10141 gcggcagaat tctcaaatcc ggtgatgctc tactctatcg gtaaagattc cagcgtcatg
    10201 ctgcatctgg cgcgcaaggc gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc
    10261 gataccggct ggaaattccg cgagatgtat gagttccgcg atcgtacggc gaaagcctac
    10321 ggctgcgagc tgctggtgca taaaaacccg gaaggcgtgg cgatggggat taatccattc
    10381 gtgcacggca gcgcgaaaca taccgatatt atgaaaactg aaggcctgaa acaggcgctg
    10441 aacaaatacg gttttgatgc cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc
    10501 gctaaagagc gaatctactc tttccgtgac cgcttccatc gctgggatcc gaaaaatcag
    10561 cgcccggaac tgtggcacaa ctacaacggg caaattaaca aaggcgaaag catccgcgtc
    10621 ttcccgctct ctaactggac cgagcaggat atctggcaat acatctggct ggaaaatatc
    10681 gacattgtgc cgctgtatct cgctgcggaa cgtccggttc tggaacgcga cggtatgttg
    10741 atgatgattg atgacaaccg tatcgacctg caaccgggcg aagtgatcaa aaaacggatg
    10801 gtgcgtttcc gtacgctggg ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa
    10861 acactgccgg aaatcatcga agagatgctg gtttccacca ccagtgaacg tcagggtcgc
    10921 gtgattgacc gcgaccaggc gggatccatg gagctgaaaa aacgtcaggg gtatttttaa
    10981 gatgaacacc gtacttgcac aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat
    11041 tgcgcaacaa cataaaagcc tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa
    11101 aagtaccctg attggtcgtc tgctgcacga tacccgccaa atctacgaag atcagctctc
    11161 atcgctgcat aacgacagta agcgtcacgg cacccagggc gaaaagctgg atctggctct
    11221 gctggtggac ggcctgcaag ctgagcgcga acagggcatc actattgatg tggcctaccg
    11281 ctatttctct accgagaagc gtaaatttat tatcgccgac accccagggc acgagcagta
    11341 cactcgcaat atggcgactg gcgcatcgac atgtgaactg gcgatcttac tgatcgatgc
    11401 ccgtaaaggc gtgctcgatc aaacccgtcg tcacagtttt atctccacac tgttggggat
    11461 caaacatctg gtcgtggcga tcaacaaaat ggatctggtg gattacagtg aagagacgtt
    11521 cacccgtatt cgtgaagatt atctgacctt tgccgggcag ctgccgggta atctggatat
    11581 ccgctttgtg ccgctctccg cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat
    11641 gccgtggtac agcggtccga cactgctcga agtgctggaa accgtggaga tccagcgagt
    11701 ggtggatgcc cagccaatgc gcttcccggt gcagtacgtt aaccgcccaa atctcgattt
    11761 tcgtggctac gccggaatgc tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt
    11821 gctgccctct ggtgtggaat caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga
    11881 agaagccttt gccggagaag cgatcaccct ggtgctgacg gatgagatcg atatcagccg
    11941 tggtgatctg ctgctggcgg cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga
    12001 tgtggtatgg atggcggaac agccgctttc cctgggccag agttatgaca tcaaaattgc
    12061 cggtaagaag acgcgtgctc gtgttgatgg cattcgttat caggttgata ttaataacct
    12121 tacccaacgc gaagttgaaa acctgccgct gaacggcatc ggcctggtgg atctcacttt
    12181 tgacgagcca ctggtgttag atcgctatca gcaaaatccg gttaccggtg ggctgatttt
    12241 tatcgatcgc ctgagcaatg tgaccgtggg tgccggtatg gtgcacgagc cagttagcca
    12301 ggcaacagct gcgtcatctg aattcagtgc attcgaactg gaattgaatg ccctggttcg
    12361 tcgccacttt ccgcactggg gcgcgcgcga tttgctgggg gataaataat ggcgctgcat
    12421 gacgaaaacg tcgtctggca tagtcatccg gtcactgtgc aacaacgcga actacaccac
    12481 ggtcatcgtg gtgtagtgct gtggtttacc ggcctctccg ggtccggtaa atcaacggtc
    12541 gccggggcgc tggaggaggc gttacataaa ctcggcgtca gtacgtatct gctggatggc
    12601 gacaatgttc gccacggatt atgcagcgat ctcggtttta gcgatgccga tcgtaaagag
    12661 aatatccgtc gcgtcggtga agtggcgaat ttgatggttg aagccggact ggtggtgctg
    12721 accgcattta tctcgccaca ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa
    12781 gggcgcttta ttgaagtgtt tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca
    12841 aaaggcttat ataagaaagc gcgtgccggt gaactgcgca actttacggg aatagattcc
    12901 gtttacgaag cgcctgaatc ggcagaaatt catctcaatg gtgaacaatt agtaacaaat
    12961 ttggtacagc aattattaga tctgttgaga cagaacgata ttatcagatc ctgagacacc
    13021 accgggtttc catgcccggt tcgtcagagt aacaggatca aatatgcgca atagccataa
    13081 cattacacta acaaataacg acagccttac tgaggatgaa gaaactacat ggtcactgcc
    13141 tggtgccgtg gtcggtttta tctcctggtt atttgcgctg gcgatgccga tgttgattta
    13201 tggctctaac acgctgttct tctttatcta cacctggcct ttttttctgg cactgatgcc
    13261 tgtcgcggta gtggtgggga ttgcgctgca ttcattgatg gacggaaagc tacgctacag
    13321 tattgttttc accctggtga cagtaggcat tatgtttggc gcactgttta tgtggctact
    13381 gggctaattt gtactttctt gtctcctctg ttcataattc aaaccgtaac taataatgag
    13441 attatgttct gcacgccctg ggtatacgta acaatggaca aatgtggtac atttgcccgc
    13501 gttgtcgcgg tatccccaac agaggatgta gagtcgtctt cggatgcatg ggatgatgat
    13561 gccgtttttc agggggcagg atgggtaaac taacgctgct gttgctggct attctggtct
    13621 ggctacagta ttcgctgtgg ttcggtaaga acggtataca tgactatacc cgcgtcaatg
    13681 atgatgtggc ggcactgcaa gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt
    13741 ttgccgaaat tgacgatctc aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg
    13801 aactcagcat gaccaggccg ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc
    13861 gcgcacagtc tgcggggcaa aacaatcgat aaatcagccc gggaattaac atggcaacca
    13921 ctcatttgga tgtttgcgcc gtggttccgg cggccggatt tggccgtcga atgcaaacgg
    13981 aatgtcctaa gcaatatctc tcaatcggta atcaaaccat tcttgaacac tcggtgcatg
    14041 cgctgctggc gcatccccgg gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc
    14101 gttttgcaca acttcctctg gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg
    14161 agcgtgccga ttccgtgctg gcaggtctga aagccgctgg cgatgtgcag tgggtattgg
    14221 tgcatgacgc cgctcgtcct tgtttgcatc aggatgacct cgcgcgattg ttggcgttga
    14281 gcgaaaacag ccgcacggga gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg
    14341 ccgaaccggg caaaaatgcc attgctcata ccgttgatcg caacggctta tggcacgcgc
    14401 tgacgccgca atttttccct cgtgagctgt tacatgactg tctgacgcgc gctctaaatg
    14461 aaggcgctac cattaccgac gaagcctcgg cgctggaata ttgcggattt catccgcagt
    14521 tggtcgaagg ccgcgcggat aacattaaag tcacgcgccc ggaagatttg gcactggccg
    14581 agttttacct cacccgaacc atccatcagg agaatacata atgcgaattg gacacggttt
    14641 tgacgtacac gcctttggcg gtgaaggccc aattatcatt ggtggcgtac gcattcctta
    14701 cgaaaaagga ttgctggcgc attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc
    14761 attgcttggc gcggcggcgc tgggggatat cggcaaactg ttcccggata ccgatccggc
    14821 atttaaaggt gccgacagcc gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa
    14881 gggttatacc tttggcaacg tcgatgtcac tatcatcgct caggcaccga agatgttgcc
    14941 gcacattcca caaatgcgcg tgtttattgc cgaagatctc ggctgccata tggatgatgt
    15001 taacgtgaaa gccactacta cggaaaaact tggatttacc ggacgtgggg aagggattgc
    15061 ctgtgaagcg gtggcgctac tcattaaggc aacaaaatga ttgagtttga taatctcact
    15121 tacctccacg gtaaaccgca aggcaccggg ctgctgaaag ccaatccgga agactttgtg
    15181 gtggtggaag atttgggctt tgagcctgat ggtgaaggtg agcatattct ggttagaatc
    15241 ctcaaaaacg gctgcaatac ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt
    15301 cattcccgtg aagtcagctt cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg
    15361 ttatgtgctc gcgtgccggg caaggaaatg cccgatctga gcgcctttca actggaaggc
    15421 tgccaggtgc tggagtatgc gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt
    15481 aacgccttta ccctggttct gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg
    15541 atcgatattt gcgtaaaagg tgtaccgaac tacttcggtg cccaacgttt tgggattggc
    15601 ggtagcaact tgcagggcgc gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc
    15661 aataaacgga gtttctggtt gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct
    15721 gagcgcctca aaaaagcaga cgttaatcaa gttgttgacg gcgatgcgct acaattagcc
    15781 ggacgtggta gctggtttgt agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc
    15841 aacgataaag agctgatgat aaccgccgca ttgccgggca gtggcgaatg gggaactcag
    15901 cgtgaagcgc tggcattcga acaagcagct gtcgccgcag aaactgaatt acaagcttta
    15961 ctggtgcgcg aaaaagttga agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta
    16021 agctggaact ggtgggatga cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt
    16081 tttgcaacca gcgttgtcag ggaacttatc aacacaacag gtgattatgc gcatattgct
    16141 gagtaatgat gacggggtac atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga
    16201 gtttgctgac gttcaggtgg tcgcccccga tcgtaaccgc agcggcgctt caaattctct
    16261 gacactggaa tcctccctgc gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat
    16321 gggaaccccg accgattgtg tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga
    16381 cattgttgtg tccggaatta acgccgggcc gaatctgggg gatgatgtta tttattccgg
    16441 tacggtagcc gccgcgatgg aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct
    16501 tgacgggcat aaacattacg acactgccgc ggcggtaacc tgttcaattt tgcgcgcact
    16561 gtgtaaagag ccgctgcgca ccggacgtat tcttaatatt aacgttccgg atttaccctt
    16621 ggatcaaatc aaaggtattc gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt
    16681 gatcccgcag caagatccgc gcggcaatac gctgtactgg attggcccgc cgggcggtaa
    16741 atgtgatgcc ggtccgggga ccgattttgc tgcggtagat gagggctatg tctccatcac
    16801 gccgctgcat gtggatttaa ctgcgcatag cgcgcaagat gtggtttcag actggttaaa
    16861 cagcgtggga gttggcacgc aatggtaagc agacgcgtac aagcacttct ggatcaatta
    16921 cgtgcgcaag gtattcagga tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa
    16981 aaattcgttg atgaagcgtt tgaacaaaaa gcctgggaca atatcgcctt gccgataggt
    17041 caggggcaga caatttcgca gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg
    17101 acgccgcagt cgcgggtgct ggaaattggc actggttcgg gatatcaaac ggcaatcctg
    17161 gcgcatcttg tccagcatgt ttgctcggtt gaacggatta aaggcttgca gtggcaggca
    17221 cgtcgccgcc tgaaaaatct tgatttacat aatgtttcaa cccgtcatgg cgatggatgg
    17281 caaggttggc aggcacgtgc gccgtttgac gctatcattg ttacggcggc accgccggaa
    17341 attccaactg cgctaatgac gcagctggac gaaggcggga ttctcgtctt acccgtaggg
    17401 gaggagcacc agtatttgaa acgggtgcgc cgtcggggag gcgaatttat tatcgatacc
    17461 gtggaggccg tgcgctttgt ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa
    17521 tacctggatt tttcctggtt attttgccgt aggtcagcgt atcgtgaaca tcttttccag
    17581 tgttcagtag ggtgccttgc acggtaatta tgtcactggt tattaaccaa tttttcctgg
    17641 gggataaatg agcgcgggaa gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact
    17701 ggtttcgcta tggctggcag gctgttctga cacttcaaat ccaccggcac cggtcagctc
    17761 cgttaatggc aatgcgcctg caaatactaa ttctggtatg ttgattacgc cgccgccgaa
    17821 aatggggacg acgtctacag cgcagcaacc gcaaattcag ccggtacagc agccacaaat
    17881 tcaggctact caacaaccgc aaatccagcc agtgcagcca gtagctcagc agccggtaca
    17941 gatggaaaac ggacgcatcg tctataaccg tcagtatggg aacattccga aaggcagtta
    18001 tagcggcagt acctataccg tgaaaaaagg cgacacactt ttctatatcg cctggattac
    18061 tggcaacgat ttccgtgacc ttgctcagcg caacaatatt caggcaccat acgcgctgaa
    18121 cgttggtcag accttgcagg tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc
    18181 cattacccag gccgacgcag cagagcaagg agttgtgatc aagcctgcac aaaattccac
    18241 cgttgctgtt gcgtcgcaac cgacaattac gtattctgag tcttcgggtg aacagagtgc
    18301 taacaaaatg ttgccgaaca acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt
    18361 accaacagca agcacaaccg agccgactgt cagcagtaca tcaaccagta cgcctatctc
    18421 cacctggcgc tggccgactg agggcaaagt gatcgaaacc tttggcgctt ctgagggggg
    18481 caacaagggg attgatatcg caggcagcaa aggacaggca attatcgcga ccgcagatgg
    18541 ccgcgttgtt tatgctggta acgcgctgcg cggctacggt aatctgatta tcatcaaaca
    18601 taatgatgat tacctgagtg cctacgccca taacgacaca atgctggtcc gggaacaaca
    18661 agaagtgaag gcggggcaaa aaatagcaac catgggtagc accggaacca gttcaacacg
    18721 cttgcatttt gaaattcgtt acaaggggaa atccgtaaac ccgctgcgtt atttgccgca
    18781 gcgataaatc ggcggaacca ggcttttgct tgaatgttcc gtcaagggat cacgggtagg
    18841 agccacctta tgagtcagaa tacgctgaaa gttcatgatt taaatgaaga tgcggaattt
    18901 gatgagaacg gagttgaggt ttttgacgaa aaggccttag tagaagagga acccagtgat
    18961 aacgatttgg ccgaagagga actgttatcg cagggagcca cacagcgtgt gttggacgcg
    19021 actcagcttt accttggtga gattggttat tcaccactgt taacggccga agaagaagtt
    19081 tattttgcgc gtcgcgcact gcgtggagat gtcgcctctc gccgccggat gatcgagagt
    19141 aacttgcgtc tggtggtaaa aattgcccgc cgttatggca atcgtggtct ggcgttgctg
    19201 gaccttatcg aagagggcaa cttggggctg atccgcgcgg tagagaagtt tgacccggaa
    19261 cgtggtttcc gcttctcaac atacgcaacc tggtggattc gccagacgat tgaacgggcg
    19321 attatgaacc aaacccgtac tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt
    19381 tacctgcgaa ccgcacgtga gttgtcccat aagctggacc atgaaccaag tgcggaagag
    19441 atcgcagagc aactggataa gccagttgat gacgtcagct gtatgcttcg tcttaacgag
    19501 cgcattacct cggtagacac ccgctgggtg gtgattccga aaaagcgttg ctggacatcc
    19561 tggccgatga aaaagagaac ggtccggaag ataccacgca agatgacgat atgaagcaga
    19621 gcatcgtcaa atggctgttc gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat
    19681 tcggtttgct ggggtacgaa gcggcaacac tggaagatgt aggtcgtgaa attggcctca
    19741 cccgtgaacg tgttcgccag attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc
    19801 aaacgcaggg gctgaatatc gaagcgctgt tccgcgagta agtaagcatc tgtcagaaag
    19861 gccagtctca agcgaggctg gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc
    19921 tggataagac acgtcagcgt cctacccggc aatcagcatt ctatgtgctc atcatcttgc
    19981 gcaccaactg cataaacagc tcctgctctt ctccactcaa acgccccaaa aattcctcat
    20041 ccacgctgtc accaataggt atcgccgcag caagtagctt ttctccttcg gcagtcagcc
    20101 agacaaagcg tcgccgcttg tcagcgacgt catgttcacg tctgactaag ccacgatttt
    20161 ccattcttgc caacatttct gctagcgttg ctttggtgct gactgctgct tctatcagtg
    20221 ccacctgttc aataccaggt ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg
    20281 tcaggtcagg taactcgtgc tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact
    20341 gatggaacgc tttatttcgt aacgccatgt aaactccggg ctaattctct gtcgctatca
    20401 tagcggtttt ttagtgacga agaagatgtt gttacttttc aatccttgcc gctgtggaaa
    20461 tgaaggagta cgttaataat aacgttcgta tacgaacaat taagagaata agcaatgaaa
    20521 ctgatcgtcg ggatgacagg ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg
    20581 ctgcgtgaga tgccgaatgt cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc
    20641 attgaactgg aaacgcctta cagcgctcgc gatgttgctg ccctcgcaga cttcagccat
    20701 aacccggcgg atcaggcggc gatcatctca tccggttctt ttcgtaccga cggcatgatc
    20761 gttattccgt gcagtatgaa aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg
    20821 gtagggcgcg cggcggacgt cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt
    20881 gaaatgccgc ttagcaccat ccatctcgaa aatatgctcg cactttcacg catgggcgtg
    20941 gcgatggtgc cgccgatgcc tgccttttat aaccatcccg aaacggtaga tgacattgtc
    21001 caccatgtgg tagcccgcgt gctggatcaa tttggcctcg aacatcccca cgccaggcgc
    21061 tggcaaggat tgccgcaggc ccggaatttt tctcaggaga atgaataatg gcatttgatg
    21121 atttacgcag ctttttacag gcgcttgatg accacggcca gttactgaaa atcagcgaag
    21181 aagtgaacgc tgagccggat ctggcagcag cagctaacgc caccgggcgt atcggcgacg
    21241 gcgcgcccgc gctgtggttt gataatattc gcggctttac cgatgcccgc gtggcgatga
    21301 ataccatcgg ttcctggcag aaccacgcga tttcccttgg cctgccacca aacaccccgg
    21361 tcaaaaagca gattgatgag tttatccgcc gctgggataa cttcccgatt gccccggagc
    21421 gccgcgccaa tccagcctgg gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg
    21481 atattctgcc gctgtttcgc ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt
    21541 gcgtggtttc ccgcgatccg ctcgacccgg ataacttcgg caagcagaac gtcggcatct
    21601 accgcatgga agtgaagggc aagcgtaagc tcggcctgca accggtgccg atgcacgata
    21661 tcgccctgca tctgcataaa gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc
    21721 tcggtaacga tccgatcatc acgctgatgg gggccacgcc gctgaaatac gatcagtctg
    21781 agtatgaaat ggcaggcgcg ctgcgtgaaa gcccgtaccc gatcgccacc gccccgctga
    21841 ccggttttga tgtgccgtgg ggttcagaag tgatcctcga aggggttatc gaaagccgta
    21901 aacgcgaaat cgaagggccg ttcggtgagt ttaccgggca ctactccggt gggcgcaaca
    21961 tgaccgtggt gcgcatcgat aaagtctctt atcgcagcaa accgattttc gaatcactct
    22021 atctcggtat gccgtggacc gaaatcgact acctgatggg gccagccact tgcgtaccgc
    22081 tgtatcaaca actgaaagcc gagttcccgg aagtgcaggc ggtaaatgcc atgtacaccc
    22141 acggcctgct ggcgattatc tccaccaaaa aacgctacgg cggctttgcc cgcgcggtgg
    22201 gcctgcgtgc gatgaccacg ccgcacggtc tgggctacgt aaagatggtg attatggtcg
    22261 atgaagacgt tgacccgttc aacctgccgc aggtgatgtg ggcgctctcc tcgaaagtaa
    22321 acccggcagg ggatttggtg cagttgccga atatgtccgt gctggaactc gaccctggct
    22381 caagcccggc ggggatcacc gacaagctga ttatcgacgc taccacgcct gtcgccccgg
    22441 acaaccgtgg tcactacagc cagccggtgg tggatttgcc ggaaaccaaa gcctgggctg
    22501 aaaaactgac cactatgctg gctgcacgta aataaggaga acaagatgat ttgtccacgt
    22561 tgtgccgatg aacagattga agtgatggtg aaatcgccgg tgaaagatgt ctggacggta
    22621 tatcagtgcc agcattgcct ttatacctgg cgcgataccg aaccgctacg ccgcaccagt
    22681 cgcgaacatt acccggaagc gttccgcatg acgcagaaag atattgatga tgcgccaatg
    22741 gtgccgagca tcccgccgct gctggcggaa ggtaagcgct aaataaaaag gtccgatgcc
    22801 catcggacct ttttattaag gtcaaattac cgcccatacg caccaggtaa ttaagaatcc
    22861 ggtaaaaccg agaatggtcg ttaacactgt ccaggttttc agaccgtctg ctaccgacaa
    22921 ccccagatat ttggtcacaa tccagaaccc tgagtcatta atatgtgacg ccccaagccc
    22981 accaaagcag gctgccagcg tcaccaatac gcactgaatc ggattcaatc ccatcaccgc
    23041 ttctgagagt aacccgccgg ttgtcagtat tgctacggtt gctgacccct gcgatgcacg
    23101 cagcgccagt gaaataataa atgcggctgg taacagaggc aggtcaatca tttgtaacat
    23161 gttggcaagg gctttgccga cgcccgattc caccagcact ttgccaaata cccctccagc
    23221 accagtaacc aaaatcacta ccgccgcagt aggaagcgct gagcccataa tgtcgctggt
    23281 gtgttgtaag ctccagccgc gacgtaaagc caataaccag aatgccagca ccagcgcaat
    23341 cattagagct accattggtg agccgatcag ctgtagcgta ccaagcaggg gatgcgaagg
    23401 cggcatcagt gttgcggaaa ccgtacccac catgataatc gcgataggaa taacaattag
    23461 cgaggtgacc agcgcgacgc ccggtggatt tattttatcg cttaattttg tcgcgccttc
    23521 ctcactggcc ggagccagtt gcatctgttc cagtacttct actgacatcg catattggcg
    23581 tttattgatt attttcgctg caaagtagcc aacaacccct acgggaatag aaatcgcaat
    23641 accgatgatg gttagccagc cgatgtctgc gtggagtaac cccgctgcgg cgacagggcc
    23701 tggatgcggc ggtaccgcca cgtgaacagt gagcatgatc ccagcgacag gcaggccaaa
    23761 tttgagtggc gatattttgg ctaccttggc aaaaccgtaa atgattggcg caagaataat
    23821 aaagccgaca tcaaagaaga cgggaatacc gaggaagaac gctgccagag tcagcgcagc
    23881 gatagttcgt ttgtcaccta acttgcgact gaaataatta gccagtgact ctgcaccaca
    23941 agagtgttcg atcatacgcc ccagcatagc gcccagacca ataataatag tgacggaacc
    24001 aagcacaccg cccatcccgg cgatcatcac tttacccact tcgcccgccg gtatacccgc
    24061 cgcaagtgcg actaacaggc tgacgaggag cagagcaacg aatggttgta cctttgcctt
    24121 gatgaccagc agcaacagca tgattacgcc agctaacgca atgcataaca atgtaattgt
    24181 ggacatggga aaccctgtct gaaagttata gttaacctac cccatccgta gatgggggga
    24241 tgtatgggta cgttgtaatt agggatttaa cgaattagcg ctaggcgtca aaccagccaa
    24301 gcccttcttc ggtgaggcca cgaggtttat attcacaacc gatccagccc tgatatccca
    24361 cctcatcgaa caggcggaac agccacggat agttgatttc tccatcgtcc ggttcatgtc
    24421 gatcaggtag tccggcaatt tgtacgtgcg catatttccc ggcgtagtcg cggattaaat
    24481 gcgtcaggtt gccatctact ttttgcgcat gaaaagtatc tagttgaata aacacgttat
    24541 ctcgcgcaac ctcttcaaca atagccagtg cctgatactg gctggagaag agataatgag
    24601 gcttaacgcc ggggctgagt gcttcaacta atattcgctt gccgtgtggc gcaaagcggt
    24661 cggcagcgta gcggagatta tcgataaata ctgcccggta ccgttcagca tcttcgccag
    24721 cgggcacgac gcctgccatc acatggactt gttcacaatt gagcgccaat gcatattcca
    24781 gtgccaggtc gatgtctgcg tgtgcttcgt gctcacgtcc gggaagggcg gataatcccc
    24841 attcccccgc attaatatct ccgggagcgg tattgaacag cgccagtgtc agatggtttt
    24901 gctccagttg cttttggatt tgcagggtgg agtagttata gggaaacaga aattccacag
    24961 catcgaaccc ggcttttcgc gctgcggcga agcgttcaat aaaaggcact tcggtgaaca
    25021 tcatggataa attagctgca aaacgaggca ttgcattaac tccttaattc cgcaatttca
    25081 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
    25141 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
    25201 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
    25261 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
    25321 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
    25381 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
    25441 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
    25501 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
    25561 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
    25621 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
    25681 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
    25741 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
    25801 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
    25861 cggaggtctc accgcctgcg acaataaagc gtgtcacgcc ttccgctgct aaccgcgccg
    25921 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
    25981 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
    26041 cctggcccag aacccactct gccagttcgt gtgcataagc ggccagagtt tcggttgaga
    26101 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctggc
    26161 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
    26221 ctgcttcgcg agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca
    26281 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
    26341 ggtgttcggt cagcgcatca agcaccgcgt agcggtagcc ctcttgctgt aagcgagcca
    26401 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
    26461 gccctgtgga ttgcgcttca accagacggg gaagatagcg gtcggtcatg ggatttaccg
    26521 ggtgatggtg catcccggat tcggccagca gttgattcat tacgaacaaa tacccctgat
    26581 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
    26641 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
    26701 agcagtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
    26761 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
    26821 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
    26881 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
    26941 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
    27001 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
    27061 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
    27121 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
    27181 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
    27241 cagcgggatc cccgcacggg tgccagtcgt tcaaaggcaa tatcgctacc ggaggccatg
    27301 acagtcattt caccgttagc ggcttttact gcaccaccag aaactggcgc atccagcatt
    27361 tccagatcga atccagccag agcggtagca atttcttgcg catcagcact agcgatagtg
    27421 gaagaaacca ttactgccgt accgggtttc agatgttgtg caacgcctgt ttcaccaaac
    27481 agcacctgtt taacctgggc cgcattgacc accagcacca gcagagcgtc cagtttttcg
    27541 gcaaacgtcg cggcgttatc agaaaccccg caagcacctg cctctttcaa cgtagcgcag
    27601 gcattgctgt tcaggtctgc gccccaggta gaaaaacctg cgcggacaca tgacagtgct
    27661 gctcccattc ccattggccc taagccaacg ataccgacat gaaactcaga tcccgttttc
    27721 atctgctctc cttgttaatt taagtgatat tttgtttgat attgtgaata tgagcgctga
    27781 aagataacga tatggtgagc tgattcacat aaattaacat tttgtgttat tttatgtgaa
    27841 ctaagcgtta gttgcgccgc gcacgtttcg caggcaaata gcgtagaatg tcagcaggac
    27901 aaaggaagga gcaaaagttg atacccgtag agcgtcgcca aatcatcctt gagatggtag
    27961 ctgaaaaagg cattgtcagt attgctgaac taacggacag aattaatgtg tcacatatga
    28021 ccattcgtcg ggatttacaa aaactggagc agcagggagc cgttgtgctg gtgtccggag
    28081 gcgtccagtc tccgggacgc gtggcgcatg aaccttctca tcaggtaaaa actgcgctgg
    28141 caatgacgca aaaagcggct attggcaagc tggcggcaag tcttgttcag ccgggaagtt
    28201 gtatctatct ggatgcggga acgaccacgt tagcgatagc acagcatctg attcacatgg
    28261 agtcactgac tgtggtcaca aacgatttcg ttattgcgaa ctacttgctc gacaacagta
    28321 attgcacaat tattcacact ggcggtgcag tgtgtcggga aaaccgttcc tgtgtcgggg
    28381 aagccgctgc gaccatgctg cgcagcctga tgattgatca ggcttttatt tctgcatcgt
    28441 catggagtgt gcgggggatt tctacgccag cggaagataa agtcacggtg aaacgggcga
    28501 ttgccagtgc cagccgccag cgagttttgg tctgtgatgc gacgaaatat ggtcaggtgg
    28561 cgacatggct ggcgttacca ttaagcgagt ttgatcagat tattacagac gacggtttgc
    28621 cggagagtgc cagtcgcgcg ctggcgaagc aggatctctc tttgctggta gcgaaaaatg
    28681 aataatggcc tgcaataaca tctggttact catgcttcac agaagaagca tgagactacc
    28741 ttattttata aaatgacagc cgcccacttt tcggcgatcc ggtatcaata taaatctggt
    28801 tagcgaacgt ctgaatgtta tcaaacatca tatgtccaaa tataaaataa tcagcgccgt
    28861 ttatttgttg taactcgcca ttaagcgatt tctgcatacg ataaacaggc cagagtaatt
    28921 cgctctccgc tatttctttg ccaaagagat attcatcacc cggataatct gcatgtgcga
    28981 tgacatattt tattatgtcg ttagtgattt caataatatg tggaaggtga tggaatttca
    29041 gcaacagatc tattgcctct tgttgctctg aatcatttaa atcgaaaaac cagtcaccac
    29101 cgctggcaag ccacatattg ccatcgccgg tcgcgaacgc atccagcgcc attgcttcgt
    29161 ggttgccttt aacggagata aaccagggct ggtttagcag gcgcaggacg tcaagactct
    29221 ccggcccacg atcaatatta tcgccgacag aaataagtaa gtcggtttcg gggaaaaaag
    29281 agagttgatg taagcgggat tgtaataact gatattcacc atgaatatca ccaacgaccc
    29341 atatatggcg atagtgatgg gcattgattt tttgatagcg tgtagatggc atggttttac
    29401 cctgtaaaat aagctttcct attatacagg gtatttttat ttgattcgtc agttgtcgtt
    29461 aatattcccg atagcaaaag actatcggga attgttatta caccagactc ttcaagcgat
    29521 aaatccactc cagcgcctga cgcggagtca gtgaatccgg gtcgagattt tccagtgctt
    29581 cgaccgcagg cgaagtttct tctggcactg acagcaaaga catctgcgta ccatccactt
    29641 gcgtcgcggc ggcgttcggc gaaatgcttt ccagctcacg cagtttttgc cgtgcgcgct
    29701 taataacctc tttcggtacg cccgccagag cagcaaccgc cagaccgtag cttttgctcg
    29761 ccgcgccgtc ctgcacgctg tgcataaagg cgatagtgtc gccgtgctcc agcgcatcga
    29821 gatgtacgtt ggcgacgcct tccattttct ccggtagctg ggtcagctcg aaatagtggg
    29881 tggcaaacag cgtcaatgcc ttaatcttat tcgccagatt ttctgcgcac gcccacgcca
    29941 gcgacagacc atcgtaggtg gacgttccac gcccgatctc atccattaac accagactgt
    30001 attcggtggc gttatgtaaa atattggcgg tttcagtcat ctccaccata aaggttgagc
    30061 gcccggaagc caggtcatct gccgcgccta cgcgggtaaa gatgcgatcg ataggtccaa
    30121 tctcgacttt ttgtgccggt acatagctgc cgatgtaggc catcagcgca atcagtgcgg
    30181 tctggcgcat ataggtactt ttaccgccca tgttcgggcc ggtaataatc aacatacggc
    30241 gctgcggcga cagattcagt gggttagcga taaatggccc attcagtact tgttcaacta
    30301 ccggatggcg accttcggta atgcgaatgc ccggtttatc aatgaaggtc gggcaggtgt
    30361 agttcagggt ataggcccgt tccgccaggt taaccagcac gtcgagttcc gccagcgcgc
    30421 tcgcgctctg ttgcaacgct tccagatgcg gcaacagcag gtcgaacagc tcttcataaa
    30481 gctgtttttc cagtgccagt gctttgcctt ttgaggtgag aactttatct tcgtactctt
    30541 ttagctctgg aatgatgtag cgctcggcgt ttttcagcgt ctggcgacgc atgtagttga
    30601 tgggtgccag atggctttgc ccacggctga tttgaatgta gtagccgtgc accgcattaa
    30661 agccaacttt cagcgtgtcc aggccggtac gttcacgctc gcggacttcc agacgctcca
    30721 gataatcggt cgcgccgtca gccagcgcgc gccactcatc cagctcttcg ttatagcccg
    30781 atgcgataac accaccgtcg cgtaccagca ccggcggtgt gtcgatgatt gctcgctcca
    30841 gcagatcgcg cagctcggca aactcgccca tcttctcacg tagcgcctgt accggtgcac
    30901 tatcgacagt ttctaactgc gcacgcagct ccggcagttg ctggaaagcg tggcgcatac
    30961 gggccagatc gcgtgggcga gcagttcgta aagccagacg tgccagaata cgttccaggt
    31021 cgccgacctg acgcagtacc ggctgcaact cggcggtgaa atcctgcaat gcgccaatag
    31081 tttgctggcg ctcaagcaac acgcgggtat cgcgcactgg catatgcagc cagcgtttca
    31141 gcatacgact acccatcggc gtgacggtgc agtcgagcac agaagccagc gtattttccg
    31201 caccgcccgc caggttctga gtaatttcca ggttacgacg tgtcgcggca tccataatga
    31261 tgctgtcctg ctcacgttcc atggtgatag aacgaatatg cggcagggtc gtgcgttggg
    31321 tatctttcgc atactgcaac agacaaccgg cagcacaaag tccgcgcggc gcgttctcga
    31381 cgccaaaacc gaccagatcg cgggtgccaa attgcagatt caactgctgg cgcgcggtgt
    31441 cgatttcaaa ctcccacagc gggcgacggc gcaggccgcg acggccttca atcagcgaca
    31501 tctcggcgaa atcttctgca tacagcagtt ccgccggatt agtgcgttgc agctctgccg
    31561 ccatcgtttc gcggtcggcc ggttcgctca ggcgaaaacg cccggagctg atatccagcg
    31621 tcgcgtagcc gaaacctttg ctgtcctgcc agatagccgc cagcaggttg tcctgacgct
    31681 cctgtaacag ggcttcatcg ctgatggtgc ctggcgtaac gatacgcaca actttgcgct
    31741 caaccggacc tttgctggtc gccggatcgc caatttgttc gcagatggca acggactctc
    31801 cctgattcac cagtttggcg agatagtttt ccaccgcatg gtagggaatc cccgccatcg
    31861 ggatcggctc tcccgccgaa gcaccgcgtt tggtcagtga aatatccagc agttgcgacg
    31921 cgcgttttgc gtcgtcataa aacagttcat aaaaatcacc catccggtaa aacagcagga
    31981 tctcgggatg ctgggctttc agcttgagat actgctgcat catgggcgta tgggcgtcga
    32041 aattttctat tgcactcatg gggttatgtc cggttccctg atattaaatg gggtgtgatg
    32101 gtttttattt tcgttgtaat cacataaggc gcacatgata ccggagttaa tcatttcagg
    32161 ggaagaagcg aacggcgagt tcttgatgct ggttccggga aagatgctgt tttgcagctt
    32221 ttgcgtgatg cttatgaagc aagtacgcaa aattgtatcg attggcgaac ccttcttctt
    32281 tagagtcata gtgcccttta agattaagat gtaatataaa tgcatcttaa tgaggtggag
    32341 gcagcatgtc cggcaagcgt atctctcgtg aaaaactgac gattaaaaaa atgatcgatc
    32401 tttatcaagc gaaatgcccg caggcgtcag cggagccgga gcattacgag gcgttgtttg
    32461 tttacgcgca aaagcggctg gataaatgtg tgttcggcga agagaaacca gcctgtaagc
    32521 agtgtccggt gcactgttac cagcctgcaa agcgtgaaga gatgaaacag attatgcgtt
    32581 gggccgggcc gagaatgctg tggcgtcatc cgatcttaac cgtgcgtcat ctcatcgatg
    32641 acaagcgtcc ggtgccagaa ctgccggaaa aatatcgtcc gaaaaaacct cgtgaatagt
    32701 atgccggaca ggcagatctg tccggcaatt tgcagttaaa tcaatgccga tttatcaatt
    32761 cccagccgct tcatccgtga cagcagggtc gtgcgtttca gccccagacg ttgcgcagcg
    32821 cctttaggcc cggcaaccac gctgttggtt tctttcagca cgcgcacaat caactgatat
    32881 tcatcttcgc cctcctgggc gacaaccgtt gcggcaggcg gcgtttcagg ctccggtaaa
    32941 gcaatatctg gcaatgacag ctgcagcacg ttgccgcgtg ttagcaatac tgcgcgctca
    33001 atgacgtttt ccagttcgcg tacgttaccc ggccactcca tgttgctcaa ggtgcgcagc
    33061 gtctcggcag gaatgctgtc gatattgcgc cccagacgac gggcaatttt gaaggtaaag
    33121 gctttcgcca gcagcggaat atcttccgga cgctcgcgta gtggcggcag gtgaatcggg
    33181 aatacgttca ggcggtaata gagatcgcta cggaactctc ggtcggcgac catttttttc
    33241 agatcgcggt tagtcgcggc aattaaacgt acgtccgtct gaatgatttt gttgctgccg
    33301 agacgttcaa actcctgttc ctgcaataca cgcagcaact tcggctgtaa ctccagtggc
    33361 atatcgccca cttcgtcgag gaacagagag cttttatccg ccagttcaaa acgaccaatg
    33421 cgctgggcgc tggcaccggt aaaagcccca cgctcatgac caaacagatc gctttccagc
    33481 aatccggcag gcatcgccgc gcagttcatt ttgaccatgc ggcgattatt acgcccactg
    33541 agattatgga tcgcacgggc aatcagctct ttacccgtgc cagtttcacc gaggatcagc
    33601 acggtactgt cactttgcgc caccatttca acttgtttaa gcacgctgta catggcttcg
    33661 ctgcggccaa taatctcgcc aaattcacta tcaacattgt tgagctgctc ggtcagggcg
    33721 aggttttcat caaccagccg ttctttcaga cgatggattt cctgataggc gagggcgtta
    33781 tcgacagcga ttgccacacg ttcggcaatc tggcgcagca aattcagatt ggtagtggta
    33841 aacactttct cttcgcattg cgccagtttc agcacgccca gcatggtgtc gccagacatc
    33901 agcggtaaca ggcacaaggt ttgaatctgg ttgccccagg tgtcgaacaa catgcgttca
    33961 tagggggcta aatcgtcccg ctcgtggaga ttgatcagca gcatctcttt acttttgaac
    34021 acgcgttcgg tgagggttcc ggcttcatcg acttcgctct gttcgtgggc gggatgttgt
    34081 ttatcaagat agtgagtgga gtagatgttg agtttgtttt tacggtggct gcgtaagaca
    34141 atgctgatat cgtcaatgtc gaaatagtaa tggatttctt tggcgacttc gctgaccagt
    34201 tcgtccatat ccaggcggga aagcaccgcg ttggtaatgg caaccaggat gcggaagtta
    34261 tcgagctccc ggcataacaa ctcatagtcg acattgttga cgacgcggct ctggatttgt
    34321 tcggtgacga cagaaacgat ctgcgtaaat gtttgcagac gattgaactc tttttcgctc
    34381 cagggacgat cgtcataacg aataaattca cagccaccaa aaatatgccc ttccgccgcc
    34441 agtggcatca ggcaatagtg accaaatttg gggtatagcc cacctgcggc caggtttcac
    34501 aaaactcttc gtaactgcaa tgcagcgtat cagggcgcga caaaatgctg cgtaccggac
    34561 cgtgtgccag aacagtttcg tcttcatatt taatgggggt gtctttttca cgcgacgcgt
    34621 aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg tcggcgagcg
    34681 cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg ggctgctgca
    34741 atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc ggtgtatatg
    34801 acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg actaactata
    34861 ggagatttag aattttatta gcatatacgc ggaagcggtt cggcgtgtgg taaatcgagg
    34921 gttcgtttca cgccatacag accggcaaga cgaacacctt tacgttccac cacttcacca
    34981 atcagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac ttgctctgcc
    35041 gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttcag ggcgtccagt
    35101 cccagcaatt cgcaaacgcc gcgcacggca ggtttaaccg gcagcgctga ttcagaaatt
    35161 tcaataccac aaccgcaggc tgccgcgaac tcatgaacca ccgcgtttac accaccacgg
    35221 gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat aagcggcgtc
    35281 agcaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg caggttaagg
    35341 atagtcgccc cgtggtcgcc gagtgtaccg ctaaccagca atacatcgcc tgcggttagc
    35401 gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt gttgataaac
    35461 agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat ggcaatgcct
    35521 gccgtgcggg cggtttctgc catgctggtc actacggctt tcagtgtctc catcggcaat
    35581 ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact gaccgcaacg
    35641 tcattcgcgg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa gaacagcggg
    35701 tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttccgctac cagctgcgcc
    35761 agatcaagac gtgcctgatc ttcctgctct gccagccacg ggttggcaaa ggcttccata
    35821 aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc gagttggata
    35881 ttattcacgc ttcactctcc tgctgacgat actgatacca cgcggcgcac gctccttcgg
    35941 aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca aacagcgggc
    36001 attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc gggtcatcgc
    36061 agacctgctg cggtgccggg cggaaatgtg cttcggcatc gaatcgttga taatccggcg
    36121 tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg tcgccgttga
    36181 cacagaacac atcggcaatc gcctgttgcg ccagcaggtt accggcatcc ggtaccaccc
    36241 gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc agcatgacca
    36301 cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga tgaaaatcgc
    36361 tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc ggcgcgagga
    36421 acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg ataagcgtaa
    36481 tgtgctggca gaagaagtaa aaattctgca catcacgcgc tttcgcctgt tgcagagtga
    36541 tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaacaccact ttgcgggttg
    36601 gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc acatcggcac
    36661 cgcgtgcttt tgcctgcaac agcgatccct gtttccctgg tacgcgcatg gcgtcgccaa
    36721 aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg attctaccca
    36781 tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt tcaggcagta
    36841 actggtcgag gccgaattta aaaatggcgt gggtatgacc gccacacact tccataatcc
    36901 gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga tgctcaatta
    36961 actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt tttcctcgcc
    37021 atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg cgtcgagagt
    37081 gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgaa ccagtaccca
    37141 ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta aatcgacatc
    37201 gcgctgaatg ccgcagacgt cgactttcgc ctggttgccg tcaatggtgc ggatctggcc
    37261 gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc catcccttcg
    37321 ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg ggcgcaggcg
    37381 atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt gagcagcatc
    37441 agcgaggcgg cggcaaacat atgccgatat ttcagcggtt tgtcttcacc ttcggtaacg
    37501 gaaagcaccg ctactttgtg tttttcaccg agatcgaagc tggccgggca tacgaggttg
    37561 ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg tgcggcgtcg
    37621 gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat cgctggtgta
    37681 ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcgccttc aataaccgcg
    37741 cacggaacgc tgtctttcag gcgcattaag gtttccgtca gcagggtggt tttaccggaa
    37801 ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg cgcgcggttg
    37861 cgttcagcca gacggttgtt tttgtccagc acgtcaattt cgacttccag catccgacgc
    37921 tggctcatgc ccggtgcgtg agtgcccgct tcgccatgac cgtaatgcag gtcgccttct
    37981 tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat ctgcgggcgt
    38041 gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc atcaccctcg
    38101 atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc ctggtctatt
    38161 tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc accatgacac
    38221 tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca tgtttcacac
    38281 cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac gctgccgcgg
    38341 caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa tgcgccaatt
    38401 ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg ttcgatcaat
    38461 tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa ctattgccag
    38521 ctacaagcaa taattgcgcc agtgttgatt atccctgcgg tgaataatgt cgatgatgtc
    38581 gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct ctttaataac
    38641 aataaattaa aggttggcgc aaaaaatgct taaagctggc atctctgtta aacgagtaac
    38701 ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca gcggcatcgt
    38761 cgtctacagg accagtggca catctactgc aattcgctgg ttcaggggat cacgttatcg
    38821 aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg tttcgtcctt
    38881 tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca caccatcgag
    38941 tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc gcaactgagc
    39001 attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt tctggaacac
    39061 tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgccattga aaacaatgtg
    39121 ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc tgaggctttg
    39181 cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg tcgaaaatga
    39241 catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc tggtgaatcg
    39301 ttttgtaatt gccgactcca cgctctgtat cggctgccac acctgtgagg ccgcctgttc
    39361 agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga tgctcaatga
    39421 aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgcg cggtggtctg
    39481 cccggttaac gccatcaccc gcgttgatgg ggccgtgcag ttgaatgaaa gtctgtgcgt
    39541 aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt ccggcagccg
    39601 tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac cgcctgctcc
    39661 ggcgcgtgtc agcacattgc ttgactgggt gccaggtatt cgcgcgatcg ccgtcaaatg
    39721 tgacctttgt agctttgatg aacaaggtcc ggcctgtgtg cggatgtgcc cgactaaagc
    39781 cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc gtgagctgac
    39841 ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg gagaggctaa
    39901 atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc cgctgttctg
    39961 gcatttctct tttcttttca aaaagcgcta agtggctgga tagccggaat tggcggcgcg
    40021 gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc ggttggcgtg
    40081 agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ccccgcttaa cgcgatttgg
    40141 ctgattaccc tcggcctttg cggtctgttt gtcagcctct ataacattga ctggcatcgc
    40201 cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc cgccgtctgc
    40261 gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat ggccttgtgt
    40321 gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct ggggcgtctt
    40381 ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg cacgctggat
    40441 ctgcgcctgc tggatatgcg tatgcaacag ctgccgctcg gttccgatat ctggctgctc
    40501 ggtgtgattg gctttggcct gctggctggg attattccgc tgcacggctg ggtgccgcag
    40561 gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt catgaaaatt
    40621 ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct gtggtggggg
    40681 atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta tgcgttgatg
    40741 gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg cattatcctg
    40801 ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct gattgctctt
    40861 ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag cgtactgttc
    40921 ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa actcggtggt
    40981 attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat ggcaatggct
    41041 gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc cttcttcaaa
    41101 ctgagcaata gtggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc tgtgggtctg
    41161 gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt cacgttcctc
    41221 ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct gatgagcgta
    41281 agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc gtggctgctg
    41341 ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac cgtttctcaa
    41401 ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat tatggcgatt
    41461 ttcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg cggctacgac
    41521 catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa acaggcgttt
    41581 gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc gggctggcag
    41641 tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc ggtactggtg
    41701 gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta attcaggcgc
    41761 tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg cgcgcccgtc
    41821 tgcataaccg tcgcgggccg ggcgtgttgc aggagtatcg cgacattatc aaactgctgg
    41881 gccgccagag cgttggcccg gatgcctctg gctgggtgtt ccgcctgacg ccgtatgtga
    41941 tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc ggttctccgc
    42001 tgccgcaact gggcgatttg atcaccttac tgtatctctt cgccatcgcg cgtttcttct
    42061 ttgccatttc cggtctggat accggtagcc cgtttaccgc tatcggtgcg agccgtgaag
    42121 cgatgcttgg cgtgctggtc gaaccgatgt tgctgcttgg tctgtgggtt gccgcacagg
    42181 ttgccggttc caccaacatc agcaacatta ccgacaccgt ttatcactgg ccgctgagcc
    42241 aaagcatccc gctggtgctg gcgctctgcg cctgtgcgtt cgccactttt atcgaaatgg
    42301 gcaaactgcc gttcgacctg gcggaagccg agcaggagtt gcaggaaggt ccgctctccg
    42361 aatacagcgg tagcggcttt ggcgtcatga aatggggtat cagcctgaaa cagctggtgg
    42421 tgttgcagat gttcgtcggg gtgtttattc cgtggggaca aatggaaacc ttcaccgtcg
    42481 gtggactgct gctggcgctg gtgattgcca tcgtcaaact ggtggtcggc gtcctggtta
    42541 tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg cgcattacct
    42601 gggctgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg tgattaaaga
    42661 gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc tgaatgaggc
    42721 atttccaggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga ctgtcaccgt
    42781 aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg gtggctggct
    42841 gtcggtgctg tttggtaacg acgaacgcaa actgaatggt cattacgccg tttattacgt
    42901 gctgtcgatg gagaagggca ccaagtgttg ggtaacggtt cgcgtcgaag ttgacgccaa
    42961 caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg gcgagcgcga
    43021 agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgtc gtctggtgct
    43081 gccggatgac tggccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca
    43141 gcgtctggca ccgactaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa
    43201 gaaaaacaac gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggtcactt
    43261 ccgtctgttc gtcgatggcg aaaacattat cgacgccgac taccgtctgt tctacgtcca
    43321 tcgcggtatg gaaaaactgg cggaaacccg catgggttat aacgaagtga ccttcctctc
    43381 tgaccgtgtg tgcgggatct gcggctttgc ccacagcacc gcctacacca cgtcggtgga
    43441 aaacgcgatg ggtattcagg tgccagaacg cgcgcagatg atccgcgcca ttctgctgga
    43501 ggtggaacgc ctgcactcgc atctgctcaa cctcggcctc gcctgtcact ttaccggctt
    43561 cgactccggc tttatgcagt tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat
    43621 ccttaccggt gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct
    43681 gctgaaagat gacatgatcc agacccgcca gctggcgcaa caaatgcgtc gtgaagtgca
    43741 ggagctggtg gatgtgctgc tgagtacgcc gaacatggaa caacgcaccg tcggcattgg
    43801 tcgtctggac ccggaaatcg cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag
    43861 cggtctcgcc cgcgatactc gcgccgatca cccgtttgtc ggttatggcc tgctgccaat
    43921 ggaagtccac agcgagcagg gctgcgacgt tatttcccgt ctgaaagtgc gaatcaacga
    43981 ggtctatacc gcgctgaata tgatcgacta cggtctggat aacctgccgg gcggtccgct
    44041 gatggtggaa ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc
    44101 gcgcggcgac gatatccact ggagcatgac cggcgacaac cagaagctgt accgctggcg
    44161 ctgccgtgcc gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gtggcaatac
    44221 cgtttccgat gcgccgctga ttatcggtag cttggaccct tgctactcct gtaccgaccg
    44281 catgaccgta gtcgatgtgc gtaagaagaa gagcaaagtg gtgccgtaca aagaactcga
    44341 gcgttacagc attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt
    44401 tatcaaaaaa gtcatcaaaa ccggcacggc gacctcgtct tatccgctgg agccgattgc
    44461 ggttgataaa aacttccgtg gtaagccaga gcagaacccg cagcagtgca tcggctgcgc
    44521 ggcttgcgtc aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccacagg
    44581 agagctggcg tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt
    44641 ttgaccgacg gcggcgatca aactgtcgca agagtacgaa ctggcggtgt ggaagaaaga
    44701 agacttcctg caacagtccc gcttcgcgct gtgcaactgc cgcgtctgca atcgtccttt
    44761 cgccgtacag aaagagatcg actacgccat tgcgctgctt aagcacaatg gtgacagccg
    44821 cgcggaaaac caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct
    44881 ggtgccgtcc gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat
    44941 ttattaggcc cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc
    45001 gccagcatga aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctatgt ttaccgcgtg
    45061 gactgcggcg gctgcaacgg ctgcgaaatc gaaattttcg ccacgctttc tccgctgttc
    45121 gatgcagaac gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg
    45181 tttaccggcg cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg
    45241 ccggacccga aaatctgtat ctcatacggt gcctgcggta acagcggcgg gatcttccac
    45301 gatctctact gcgtgtgggg cggtacggat aaaattgtcc ctgtggatgt ttatatccct
    45361 ggctgcccgc caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg
    45421 gagcagaaaa ttcacgcccg tgggccgggt gaactggatg aacaaccggc ggagatcctg
    45481 catggcgata tggtgcagcc gctgcgcgtg aaagtggatc gcgaagcacg tcgcctggcg
    45541 ggttatcgtt acggtcgtca gattgccgat gattacctta cacagttagg gcagggcgaa
    45601 gaacaggttg cacgctggct ggaagcggaa aacgatccgc gtctgaacga gattgtcagc
    45661 catctgaatc atgttgttga agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc
    45721 aactgagccg taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg
    45781 tctattacag cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc
    45841 tcacctgccc atgggatgaa tatctggcat ggattgccac tctggaggcg ggcagcgaag
    45901 cccgccacaa aatggaaggc gtaccgaaat atggcgagat cgtcatcgac attaaccatg
    45961 tgccgatgct ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagcagg
    46021 aatggagtac aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt
    46081 tgatggtgag gagactgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc
    46141 gatgatggcg ctggcccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg
    46201 gtggtgattg acggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc
    46261 ccgacacgac tgctgattgt cgacgccacg gatatggggc taaaccccgg cgaaatccgc
    46321 atcatcgacc cggatgatat cgccgagatg tttatgatga ctacccataa catgccgttg
    46381 aattacctta tcgaccagtt aaaagaagac attggtgaag tgattttcct cggcattcag
    46441 ccggatatcg tcggctttta ctacccgatg acccagccga ttaaagatgc ggtagaaacc
    46501 gtttatcaac gactggaagg ctgggaagga aatggtggat ttgcgcagtt agcggtggaa
    46561 gaagaataat tctccatcag ggaattcagg gaattgaaca tcagaacagg gatgtgcgct
    46621 tttttattga gcttagtgct gcctgcacag gccaccagct ttaccgaata tctgccaatg
    46681 agtgacagcg aatacgcgca aaagcgggcg ctgaaacctc tcctgaccat gccttatgat
    46741 gccgagcaaa cctggcattt taggaaagtg ggcgttgctg gcgtgacgct tgagaagatg
    46801 ccaaatgaag aagattactg gcaattaacc gctaaagacc gcgccggtaa atcctggact
    46861 gtgcctgtgg gtatgctggc aaacatagcg ggcaaagggc aactctatcg cgccgatctt
    46921 gatcgtaacg gcattcagga tctggttatc tggttatctg gttaccaagt accggtaatg
    46981 gtctggctcc ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg
    47041 tctttgaacc caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt
    47101 tgcaaggcaa tggtcacacg caattgctgg atatgcagtt taattctggt tactggataa
    47161 ccagtctgta tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttgacaagt
    47221 taagctatcc cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaacc
    47281 ccatcgccgg gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa
    47341 agggggatgt gcttgagggg tgaaccagaa ctgacttttt gcagagtggc tatgaggatt
    47401 ttgggggtaa ttatatcgcg cacaagtgtt tacatattgt gaacttggat ttgatgagct
    47461 atagtttccg aacacataag gatagaactc gcagatgtgg ctgtttactt actataagga
    47521 agttaaaact taaaaatgca catcatttct aaagcgccat ttgaagagtc tgcgcgtaaa
    47581 tatcctaacg acgctctggc tcttcaggca ttgtatcggg ttattaagga aacggatttt
    47641 tctacgccag aagagatgcg aacggcgttt cccaacctcg acaattttaa atatcgcaac
    47701 aaatggtacg ttctggatgt aggtgggaac aacctaaggg tgattgccta cataaacttt
    47761 gttaataagc ggttctttgt gaagcacata actaatcacg ccgagtatga caaactgaca
    47821 cgttactaca gagagaacaa agaatgactg ctaatgctgc acgcgctgtt aaagcaacaa
    47881 gagaactggt caatgcagtc ccctttctgg gaggtagtga ctcagaagat gattatcgtg
    47941 aagcactgga actggtcgaa tatttgattg aagaagatga caccaatcca cttatcgact
    48001 ttctggcgag ccgcattgca gaatatgaaa acaacaacca agagtttgca gagttcgaca
    48061 aagccgttgc ggcaatgcct gttggcgtgg cgttacttcg cacgctgatt gatcaacata
    48121 atctgaccta tgcggatttg aagaacgaaa tcggctctaa atccctggtc agtcagattt
    48181 tgtctggaca acgatccctg acgatatccc acattaaggc actttcagcc cgatttggcg
    48241 ttaagccgga atggtttctt tgatttttga ctaatgccaa atgctaaatt gcccgatgcg
    48301 ctgcgcttat cgggcctaca tgtcgcacga actatgtagg ccggataagg cgttcacgcc
    48361 gcatccggca ctgttactta ctctaaatct tccccattac tggcaatcac ttttttatac
    48421 caccagaacg atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct
    48481 acgtagacaa aaccgtagcg tttgctcatt tcacccgtag aggcggaaac taaatcaata
    48541 cagccccatg tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg
    48601 cggatatgtt cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca
    48661 agttcatctt ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacgatcg
    48721 tacatcatat tcatggtgat acgcagaccg agtggatcaa ttccccagcc ccagtcgctc
    48781 acttgcagat acgggttacg cagcgatttc accacgttcg ccgcactgct gttgttggcg
    48841 ttcatctccg ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg
    48901 tttttcagga tctcatcatc gcccagcgct ttatctatgg ttaccccttt ttcgcgaaat
    48961 acgcgggcag agtaagccgg atacgcgccg cgcgcctgca catcgataaa aaacaggttt
    49021 tcccgatctt tctccagcgc cgcccagaca tcttccggtt tgcagctgta agggtagaag
    49081 ttaccgcccg ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg
    49141 gtggctagcg cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga
    49201 ttttcacctt cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg
    49261 atttcattga aggttagcca gtatttcacc agaccatcaa atgcttcaaa gcaggttctg
    49321 gcgtagcggc tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc
    49381 agatgcatcg gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta
    49441 cactcttcaa agacaggacg ataaaaagca atgccctgct gattgggcgt gatttcatcg
    49501 ccctgcggaa agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct
    49561 gccatcaggg cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga
    49621 taaaactcgt catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga
    49681 tgctcgccgt gtgggatcat atcgacggtg gtcagccctt ttccgccttc acggaacgca
    49741 ccttcagact ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac
    49801 attttcatcc tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct
    49861 ttcgcgcctg ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga
    49921 ggataaacga cagcatcacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg
    49981 gattcgccgg atcgaagaat tgcacgctgg taaatagccc cggcgctgcc attgagtggc
    50041 tggcaagccc cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga
    50101 taagcggacg tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca
    50161 taatggctga tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc
    50221 acggcaccgc cagtgatgaa ccgcccagcg acaggttagc gccgatctct gatggcatga
    50281 ccattccttc tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt
    50341 gcatcccggt cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc
    50401 ccagataacc atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa
    50461 tcgggccaat cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga
    50521 ggaagttttt ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc
    50581 accaggtcat caccagcgcc gggatcaccg tgtaggtgta tttcaccgcg gtgaccggga
    50641 tcagggcaaa ttcgacatgt tcaccctggg ccgctttcgc cattaattca ataaaactcg
    50701 gatgtaccag cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg
    50761 cggcagatgc cgcgaccatt agcggcagga agaagaaagc accgtcacca atcacattca
    50821 gaatggttaa ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca
    50881 gcagtttgac catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca
    50941 gcgcatcgag gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct
    51001 gtgcgggctg catatctccc ggcagcaggc tgacgacttc ctgaaaggcc tgcgaaaccg
    51061 tattgccgat aattacctga cactggttgt cactacgtac cacgccgagc acgccgggga
    51121 tggtttttaa cgtcgggctg tcgataagct ggtcatcttt gataacaaag cgcaaacgcg
    51181 tcatacagtg cgtgactgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc
    51241 gtgccagcgc cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg
    51301 tagttgttat cagtcgacgt gacggaaaaa tgcctatgct gaaacggaat taagaaaccg
    51361 gtttcacaaa atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt
    51421 tatatttgtg aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc
    51481 aaacggtgca gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc
    51541 tggaagtggc gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta
    51601 atggctacgt cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcagtt
    51661 accgcccaaa tttgctggcc cgcaatctgt cggcgaagag tactcagacg ctggggctgg
    51721 tagtgaccaa cacgctttat cacggcattt attttagtga attactgttt catgccgcgc
    51781 gaatggcgga agagaaaggg cgacagttgc tattggcaga tggtaaacac agcgccgaag
    51841 aagagcgcca ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc
    51901 cgcgtttttt aagcgtggat gagattgatg acatcattga cgcgcacagt cagccgataa
    51961 tggtgcttaa tcgccgcctg cgcaataata gcagccatag cgtctggtgc gatcataaac
    52021 agaccagctt taacgccgtg gcagagttga taaacgccgg gcatcaggag attgctttcc
    52081 ttaccggctc gatggattcc cccaccagca ttgaacgtct tgccgggtat aaagacgcgc
    52141 tgtcgcagca tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg
    52201 ccagcggggc cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag
    52261 ttgccagtaa cgacgatatg gcgataggtg cgatcaaagc gttgcacgag cgcggcgtag
    52321 cggtgccaga gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg
    52381 ttccggcgct ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac
    52441 ggctgatttt tatgctcgat ggcggggatt tctcaccgcc gaaaaccttt agcggaaaac
    52501 tgatccgccg cgactcactc attgcccttt cgcgataaac attgtcatca catgtcatcg
    52561 tcactagtgt cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca
    52621 gcaaattaaa aactggcatg atttgtgaat gtatcggcgc attaactgtc attgctggag
    52681 aatttgatga accgtttcat cattgctgac gcgagtaaat gtattggttg ccgtacctgt
    52741 gaagtggcgt gcgtggtttc tcatcaggaa aatcaggact gtgcatcgct gaccccggaa
    52801 acatttttac cgcgtatcca tgtcattaaa ggtgtgaaca tttccacggc gacagtttgc
    52861 cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga atggtgctat cagccgtgat
    52921 aaagggtttg ttcatgtgat gcaggaacgt tgcattggtt gcaaaacctg cgtggtggct
    52981 tgcccgtatg gcgcgatgga agtggtggta cgtcctgtga ttcgcaacag cggcgcgggt
    53041 ctgaatgtac gggctgacaa agccgaagcc aataaatgcg acctgtgcaa ccatcgtgaa
    53101 gacggcccgg cgtgtatggc ggcttgcccg acccatgcgc tgatttgtgt cgatcgtaat
    53161 aaacttgaac aactgagcgc agaaaaacgc cgccgtacgg cgctgatgtt ctaaaaatct
    53221 tacagaactg ttctggcatc aaaggaattc ccggaaatat tcgtagaata cggagcgggc
    53281 ggtatcgccg acctgagact gccggatacg acgctgtcgt gtccggcagg gcgggttacg
    53341 caattcatga tggcgggaat aactcaatgg caaaaaacac atcttgcggt gtccaactgc
    53401 gtattcgtgg caaagtgcag ggcgtcggtt ttcgtccgtt tgtctggcag ctggcacagc
    53461 aattaaatct tcacggcgat gtatgtaatg acggcgatgg cgtagaagtc cggctgctgg
    53521 aagacccgga aacgtttctt gttcaattgc atcagcactg tccgctactg gcgcgtattg
    53581 atagcgtcga gcgtgagccg tttatctggt cacaactgcc caccgagttc accatccgcc
    53641 agagcaccgg cggcaccatg aatacgcaaa ttgtcccgga tgccgccact tgccctgctt
    53701 gccttgccga aatgaatacc ccaggcgagc ggcgttatcg ttatccgttt atcaactgta
    53761 cccactgcgg cccgcgtttc accattattc gcgcgatgcc ttacgaccgc ccgtttaccg
    53821 tgatggcggc gtttccgcta tgtccggcct gtgacaaaga gtaccgcgac ccgctcgatc
    53881 gtcgcttcca cgcccagccg gtggcctgcc cggagtgtgg cccgcatctt gaatgggtaa
    53941 gtcatggtga acatgcagaa caagaggcgg cattacaggc agctatcgca cagttaaaaa
    54001 tgggcaacat tgtcgccatc aaagggattg gcggatttca tcttgcctgc gatgcacgta
    54061 acagtaacgc ggtggcgaca ctgcgggagc gcaaacatcg cccggcgaaa ccgctggcag
    54121 tcatgttgcc agtggcagaa agtttaccag acgctgcgcg ccagttgctt accacgcccg
    54181 ctgcgccgat tgtgctggtg gataaaaaat acgtacctga gctttgtgat gatatcgccc
    54241 ctggccttaa cgaagtcggg gtgatgttgc ctgcgaaccc gctccagcat ttgctgttac
    54301 aggaactgca atgcccgctg gtgatgacct ccggcaacct gagcggtaaa ccaccagcta
    54361 tcagcaacga acaggcgctg gcggatttgc agggcattgc cgacggattc ttgctacata
    54421 accgcgacat cgtgcagcgg atggatgatt cagtggtgcg cgaaagcggc gaaatgctgc
    54481 gccgttcgcg ggggtatgtg ccggatgcgc tggttttgcc tccgggcttt aaaaatgttc
    54541 cgcctgtgct gtgtcttggc gcggatctga aaaatacctt ctgcctggtg cgcggtgaac
    54601 aagcggtgtt gagtcagcat ctgggcgatt taagtgacga tggcatccag atgcagtggc
    54661 gcgaagcgtt acgcctgatg caaaacatct acgatttcac tccgcaatac attgtgcatg
    54721 atgtgcatcc gggctatgtc tccagccagt gggcgagcga aatgaatctg ccgacgcaaa
    54781 cggtactgca tcatcatgcc catgcagcgg cgtgtctggc agagcatcag tggccgctgg
    54841 atggcggtga tgtcattgct ttgacgctcg acggtatcgg tatgggggag aatggcgctt
    54901 tgtggggcgg cgagtgcctg cgggtgaact atcgcgaatg tgagcacctg ggcggcttgc
    54961 ctgcagtggc gcttccgggt ggcgatttgg cggcgaagca gccgtggcga aacctgctgg
    55021 cgcagtgcct gcgctttgtg ccggagtggc agaattaccc tgaaacggca agtgtgcaac
    55081 agcaaaactg gagcgtactg gcgcgggcca ttgagcgtgg aattaacgcg ccgctggcat
    55141 catcgtgtgg gcggttgttc gatgcagtgg cggcggcact gggctgtgcg ccagccacgt
    55201 taagttatga aggtgaagcg gcttgtgctc tggaggcgct cgcagcctca tgcgacggag
    55261 tgacgcatcc ggtgacgatg ccgcgggtgg acaatcaact ggatctcgcc actttctggc
    55321 agcagtggct gaactggctg gcaccggtta atcaacgcgc gtgggcgttt catgatgcgc
    55381 tggcgcaggg ttttgccgcg ttgatgcgtg agcaggccac gatgcgtggt atcactacgc
    55441 tggtatttag cggcggggtt attcataacc gtttgctgcg tgcacgtctg gcgcattatc
    55501 tcgctgattt cacattgctc tttccacaga gtttaccggc gggtgatggc ggtttgtctc
    55561 tggggcaggg ggttattgct gcggcgcgtt ggttagcggg tgaagtccag aacggataaa
    55621 gcgtgttgca tttggacacc cttgccggat gcggcgtaaa tgccttatcc ggcctacaca
    55681 tgcgtccccg taggtcggat aagacgcgcc agcgtcgcat ccgacatttt aggcacagta
    55741 gcccacctac accggcaacg ttttcaacaa tacaaatgcc tctttcatcc gatcctcact
    55801 gaccacaaag gcgcgaagct ggtcagcatc gtcaacgccg cgcgccacca ttccctggcg
    55861 ttcggtatta atctgccagc gtaaatcctg acgctgggtt tcgcctgcca gatgcagcgg
    55921 taattccggc gttttgattt tcaccagcat cgccggtagt ttcagcggcg tgttattgcc
    55981 gagaagattt tttgccagca ccatcccgct aagttgaatc ggctgcagga acggcaatac
    56041 ctgaccgtta atttccgcgc aatcgcccag cgcgtaaata tcggcattac tggtttgcag
    56101 ataactatcg acgcaaacgc cgcgattaat cgtcagcccg gcgcgtcgtg ccagggctgt
    56161 ttccgggcgc agtccggtgg cggcaattac cgcatccact tcgatactac gctggtggtc
    56221 cagcgttgcc agaatgccag aatccgtttt ttccagcccc tgcaactgag atttcaacag
    56281 cagatgaacg cccatccccg tcaaccgatg ctgcaagcgg ctgcttactt ctggtggcat
    56341 taatgacgcc agaatactgg cagcgttgtc gattagcgtg accgctttgc ctgcacgaca
    56401 aaaatccatc gccagttcgc taccaatcaa accaccgccg acaatcaaca cgcgtcgggc
    56461 atcccgcagt tgcgtttcac aggcgcgata ctcttgctga ctatttaacg tcagcattaa
    56521 ctcacgccca ggcacaggcg ggacaaaggc gctggcaccg gttgccagca ctaacttgtc
    56581 gtattgccac tgattattct ggcttttcac cacatgggct tcggcatcga tatccgtcac
    56641 ccaggtgtgc ggaaacaggc gcagattaaa ctgcccggca aattcacccg ccgtctggcg
    56701 ggtaaggtcg tcggtacgtt gcccctgact gataacatgg ctgaggtcgg gtttgttgta
    56761 ctcatctatg ctgtcggcgg caatcagggt taatggaata gtggcgtcct gtttgcgaat
    56821 atttttcacc agttggcggg cggcgaagcc cgaaccgatg atcacaatgc cgttgctcat
    56881 tttgcctccg atgccagttc gtcaaagacg tctttgccga gggagcattc cgggcagagg
    56941 aagttatccg ggacttcact ccacggcgtt cctggcgcaa cgtcctgcat tggctcgcct
    57001 tttgccggat cgtaaatcca ctggcagacg ctgcactgca ttcgtgggcc gaggtcagcc
    57061 gtcgtcgtgg cagaggtttc ttctttaact accgtattca ccgtgctctg cggcagcggc
    57121 gcgagcgccc actgacgggc gatttcgcga ccgtgttcac ggcataactc cagagcgtcc
    57181 tggtctggtc gccatttggc tttcaggctg agcgacattt cgaaacccgc atcctgcagg
    57241 cgcgtggaaa ggcgatccac cgcaccgccg ctccagccgt gagatccgaa agcactggcg
    57301 cgtttgttac ggaagcgtaa tccggtcatc tcctccacca gcccggcgat tttcggcatc
    57361 atcacgttat tcatcgtcga agtgccgacc agcacgcctt ttgagcggaa gacattggtc
    57421 aggatttcgt ttttatcgct tcgggcgacg ttgaaaattt tcaccgccac gcgtgggtcg
    57481 gtttccgcaa ttccctgggc gatagcgtca gccatcatgc gggtgttatt cgacatggtg
    57541 tcgtagaaaa tggtgattct gtcttcctga taatcagcgg cccacttcag gtacagctcg
    57601 acaatttgcg tcgggttatc gcgccagacc acgccgtggg aggtcgctat catatcgact
    57661 ggtaagttaa agcccaggat ctcggtaatt ttcggcgtta ccaggcggct gaacggcgtc
    57721 aggatattgg cgtagtaacg ctggcactgc tcgaaaagct ccgtctgatc cacttcatcg
    57781 ttgaacagat gctcgtcgca gtagtgctga ccgaaagcat cgttactgaa cagcaccgcg
    57841 tcgcctgtca ggtaagtcat catgctgtcc ggccagtgca gcattggtgt ttcgacaaaa
    57901 atgagctgtt tgccgttgcc gatatccagc gtgtcgccag ttttcaccac attaaaattc
    57961 cactccggat gatggtgatg accatttatc gagtcgatag cgttggcagt acagtagatc
    58021 ggcgtatcgg gaatttgcgc catcagttcg gtcagcgccc cggcgtggtc ctcttctgca
    58081 tggttaatca cgatgtaatc gatatccgcc agatcgattt cattacgcag gttctgcaca
    58141 aattcgcggc tgaatttatg gtcgacggtg tcgatcagca cgtttttttc ttcgcggatg
    58201 aggtagctgt tgtagctgct gccgcgcagc gttttatatt ccgtgccgtg aaaatcacgc
    58261 acttcccagt cacgttgacc cacccaatga atgttatttt tcaccacaat agacatagca
    58321 acctcaattt attcagcgtg ttctaaaaag atgtcttgct aattgcagat tgcgtgccag
    58381 tttcttatct gattgttttt actctataaa taaaattatg ctcattaatg agtagtcaaa
    58441 atgactatca atcaaatggt cgatatgaca atatctatag tcaaattgac agtgaggcaa
    58501 agatgagttt ttccgttgat gtgctggcga atatcgccat cgaattgcag cgtgggattg
    58561 gtcatcagga tcgttttcag cgcctgatca ccacgctacg tcaggtgctg gagtgcgatg
    58621 cgtctgcgtt gctacgttac gattcgcggc agtttattcc gcttgccatc gacggtctgg
    58681 caaaggatgt actcggtaga cgctttgcgc tggaagggca tccacggctg gaagcgattg
    58741 cccgcgccgg ggatgtggtg cgctttcccg cagacagcga attgcccgat ccctatgacg
    58801 gtttgattcc tgggcaggag agtctgaagg ttcacgcctg cgttggtctg ccattgtttg
    58861 tcggacaaaa cctgatcggc gcactgacgc tcgacgggat gcagcccgat cagttcgatg
    58921 ttttcagcga cgaagagtta cggctgattg ccgcgctggc ggcgggagcg ttaagcaatg
    58981 cgttgctgat tgaacaactg gaaagccaga atatgctgcc aggcgatgcc acgccgtttg
    59041 aagcggtgaa acagacgcag atgattggct tgtcccctgg catgacgcaa ctgaaaaaag
    59101 agattgagat tgtggcggcg tccgatctca acgttctgat cagcggtgag acgggaaccg
    59161 gtaaggagct ggtggcgaaa gcgattcatg aagcctcgcc acgggcggtg aatccgctgg
    59221 tctatctcaa ctgtgccgca ctgccggaaa gtgtggcgga aagtgagttg ttcgggcatg
    59281 tgaaaggagc gtttactggc gctatcagta accgcagcgg gaagtttgaa atggcggata
    59341 acggtactct gtttctcgat gagatcggcg agttgtcgtt ggcattgcag gccaagctgc
    59401 tgagggtgtt gcagtatggc gatattcagc gcgttggcga tgaccgtagt ttgcgggtcg
    59461 atgtgcgcgt gctggcggcg actaaccgcg acttacgcga agaggtgctg gcagggcgat
    59521 tccgcgccga tttgtttcat cgcctgagcg tgtttccact ttcggtgccg ccgctgcgtg
    59581 agcggggcga tgatgtcatt ctgctggcgg ggtatttctg cgagcagtgt cgtttgcggc
    59641 aggggctctc ccgcgtggta ttaagtgccg gggcgcgaaa tttactgcaa cactacagtt
    59701 ttccgggaaa cgtgcgcgaa ctggaacatg ctattcatcg ggcggtagtg ctggcgagag
    59761 ccacccgcaa cggcgatgaa gtgattcttg aggcgcaaca ttttgctttt cctgaggtga
    59821 cgttgccgcc gccagaagcg gcggcggtgc ccgttgttaa gcaaaacctg cgtgaagcga
    59881 cagaagcgtt ccagcgtgaa actattcgcc aggcactggc acaaaatcat cacaactggg
    59941 cagcctgtgc gcgaatgctg gaaactgacg tcgccaacct gcatcggctg gcgaaacgtc
    60001 tgggattgaa ggattaaata atcccggcct gatagaaatc ctgcaggtta attgcgccgg
    60061 tgagtttgcc gttttcatct accactggtg cggcagtgat tttgcgtttc atcagaactt
    60121 ctttggcgtc gatggcgcga ctttgtgcct gtaatgtggt gccgcctgtc gtcatcgctt
    60181 cattaactgg cgtggtgagt gcgccgccgc ctaccagcca gcgacgtaaa tcgccgtcgg
    60241 taaagacgcc ttgtacctgt tgttgagcgt cacataccgc caccagcccc agaccggtgc
    60301 ggctgagttc cagcatcgca tccatcacgc tggcggttaa cgctacctgt gggatagcgt
    60361 catcacgacg catcagatga tgcactttat tcagcaagcg agcgcccagt gccccagctg
    60421 ggtgggagcg ggcaaaatct tcttcattaa aaccgcgcgc ctgcatgacc gccatcgcca
    60481 gcgcgtcacc catcatcagg gtattgacgg tgctggaggt cggcgcaagg tgcatcgggc
    60541 aggcttcgcg ttctacggag atatccagca ccgctttcgc cgccaggccc agcggtgacg
    60601 tcggtttgcc ggtcatcgcc agcagcgcga tagatttatc ttccagacgc ggaataatca
    60661 gatccagttc cttcgcgcca ccggagtaag agataaacag catcacatcg cggctttcaa
    60721 tcatccccag atcgccgtgc agtgcttctg ccggatggac aaaaaaagcc ggtgtgccgg
    60781 tactggcaag tgttgcggcg attttcttac caatgtggcc cgatttgcca attcccgaaa
    60841 ccaccacttt gccttcgcag tgcaggatga tattggcggc gcgaacaaaa tcatcgccca
    60901 gacgttccgg taaacggctt gcttcctgca actccagcat taacgtctga cgtcccgcgt
    60961 tcagtagtgc ttcactcatt gctctctccg gttatgatca catcgatccc tttctcttcc
    61021 agcgcctgac gaaacgccgg atcgatacct gcgtcggtaa ttagcttatc gacgctttca
    61081 agactgcaaa ctacgttggg gcttttacgg ccaaactttg atgagtccgc catcaaaatc
    61141 acttcgcgcg cggcattgca cattgcctta ctgacggtat aaacctcgtt gaacgtggtt
    61201 acgcccgcat tgagatcgat gccgtcggtg cccataaaca atttatcgaa ggtgaaatgc
    61261 tcgaaggcat tctctgccag ttgcccgtga aatgaggccg attttttgcg aaatgttccg
    61321 cctggcatca ggatagtttg ttcgttatcc agttcggata gcgcattgac gatatgcagg
    61381 ctgttggtca tcaccgtgat gttattaaag cgcgagagca ggggaaccat ctgcaaaacg
    61441 gtactgccag catcaagaat gatcgaatcg ccatcatgga taaaactaac ggcagcttct
    61501 gcaatcagct ctttcttgtg ggtgttgatg agcgttttat gatcgatagg cggatcggat
    61561 tcctctttat tcaacaccac tccgccataa gtacgaatga cggttccggc atgttccaga
    61621 atgaccagat ctttgcgaat ggttgtgcct gtggtgtcaa agtattgcgc caattcttca
    61681 accgagcatt taccctgctt ttgcagatac tccagaatgg cggcctgacg ctgacgaggt
    61741 ttcatgggcg tgattacctg atttaggttt caaaatgata acttcgcaag caagtaacat
    61801 tcacaacgaa attatccacg tttcagttta agcgccaatg atagagcatt ctgtgatagt
    61861 ggatcatggg gaaagatcac atcgggctgt aaatcacccg catgcatgcc ggtaattgcg
    61921 ggaatttttt gcggtcgggc gaagacagtc agtcctttca taaacaaggt gtcgacgatg
    61981 cactgctgat cgtccagcgc gatggcgacc acgacccgcg gtttaaagcg cccgctggaa
    62041 cggcccacgc caacccgccc ttgctggcat agtgtgtcga aggcacggtt aaaacgagaa
    62101 atttgccagc cgcctaaggc cagttgcgca cactaggcga taacggcgac ggtgatgagt
    62161 gcggatacca taacttttcc ttaatgtagt ggcggaagtc ctgtatgcct gatgagcgta
    62221 gcgcatcagg cattctatag tctcacggag atccttaacg gatctccgct gttgatcaga
    62281 acatcacctg accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac
    62341 tggcgtagaa cagcagcata ttcagcacat cttgataatc gcagccgcgt ttgagcggta
    62401 ctttgtcgat gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt
    62461 gtggcaacag tgactggaac atcggcgatt tcagcaggtt accgagcatc agtgaatgca
    62521 ccgtaatgcc gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa
    62581 atttcgctgc gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga
    62641 tctgaataat gcgaccctga atcccgtcgc ggatcatcaa acgcgaaaat tcacgggcac
    62701 acaggaaata acccaccaga ttcacttgta gcgaacggtc aaaatcgccg agctggaagt
    62761 cgctgataaa ggctgctttt gctattccgg cgctatagac cagcaaatcc acgcgaccaa
    62821 agatttcatc taccccacga gagagcgcca gaacgctttg ctcgctagtg gcgtcagcac
    62881 caaaaccgta cgccatacct tcaccatatt cggcgttaat ttcttgtgcc acatttgcgg
    62941 ctttgtcgct ctgaatatcg acaaccgcga cgcgataccc ctcggcagcc agaccgtggc
    63001 acaggaacgc gcctaaggtt tgcccaccac cgatgacaac ggcaacctga ttcatttttt
    63061 actccttaac agattcaaac ttcaaaaccg atcccggcgc gatatcgtcg ggtacagggc
    63121 ctgccacatg gacagtgccc ggaaattccg cttcgtttaa accatcgaag cgcagggtga
    63181 catgacccag ttcgcgaagg ttgtcttccg ccacgctgcc aacagcggtc accggatagc
    63241 gatgctgccc gagtgaaaat tgcaaaccgg gatggagtgc acctttcaac tcgccgtggc
    63301 aatgaatgaa gcaatactct tcgaggtccg ctggcgcgcc ttcacgaaag gtgatgagca
    63361 tctggtcgct gagggcgtca gtggcactcg cgccgatacg ggtgatggtg gtctgataaa
    63421 taacggtcat gtttcagcct ctattgatag ataaaaccgg agacaaacca ggcgatcagt
    63481 acagtcggcg cgccggttaa aaagcggctc accagtacag aagggacacc gacgcgaacc
    63541 gtgtcctgac gggcttccgc cagcgacaaa ccgaccggga tgaagtcgca ggccgcctgc
    63601 gcgttgatgg caaacagtgc cggtaaagcc agatgcggcg gaatattgcc gagaccaatc
    63661 tgcacgccaa tcaatacgcc gataacctgt gcgataactg cgcctgggcc gaggaaaggt
    63721 gaaagcagtg ggaaggagca gatgagcgcc agcatgacca gacccagtgg atggctcgcc
    63781 agcggagcaa gaccgtgggc aatccagtca ccaaggccag aagccataat gatgccaatg
    63841 agcgccgaga cgaatgccat aaacggcaga atggttttta atacagtgtc gatggtgtca
    63901 cgaccagact gaaacaacac ggcaacggcg gaccccatgc ccattcccac cttcgccagt
    63961 aaaccatcgc tttgttcggt gattttctta ctggtgtcat agtcacggcc cacggaagag
    64021 ggttgtggtg tcgcatcacc cactacagtg atgttttctt cttttacgcc agagacataa
    64081 atatcttcca caatgtactg cgccagcgga ccggacttgc ccgtcgagtg gatattaatg
    64141 gtgggaatac gtcgtttcgg atagatgccg cagcgtaatg tgccgccaca gtcgattacc
    64201 gcgacaccaa tttccgcctc cgcgggttca ccttctttaa atccgtcaat agcctgccag
    64261 ccagtaagtt gtgccagttt gtcaacaatc gcaggccggg taccggcggt gatatagacg
    64321 atttttttgc ccggcgtggc ttccagctca agcgggccgc cccagccacc cgttcctttt
    64381 tcgatccgaa tacgcgtcat gatgttgctc ctgccaggtg aactttttgt tcaagttgaa
    64441 tgcccatctt tttctcaaaa atggcggtgg tcagatcggt cacccagccg cggaagaaat
    64501 tggtgaccag accaaccagc agataactca ccgccagtgg gccaagtggc aggttcagcg
    64561 ttgtcagacc gctggcaatg ccaagataaa caaacagttc gccagggttg atatggggga
    64621 agaggccatt cattgagtgg cagctataag aggccgccgc gtagtagctg ggtttgtact
    64681 tttccggcat aaagcgaccc aggcttaagg tcatcggatt gcaaaagaca aacgtgccaa
    64741 tgcacggtaa cagtaggtaa cgggaaacag ggttaccggc gcaacgttga gcaaaacgtt
    64801 caatacgatg ctgaccgata aaattaatca gtgcgttcat gataaccagc aggctaatca
    64861 acagcggaag aatgccggtc accatcccgg taaacacctc tccgcccttt tggaacagcc
    64921 cgataaacca ctctgcacca tgagtaatgg tttctatcat tgttctctcc ttcaggattt
    64981 attgttttat taccaaatgg caacctaata taatcagatt gaaaggttta aaagtgttat
    65041 tttgatcgca aaatgaaaga taaatatttt aatttgaaag tttgaataaa agtatagcga
    65101 ggggaatgag ttgagttatg taaagtccgt atcgggcagt gactaccgct tccttgtgcg
    65161 gggcgtgatg ctttaccata cttgcccctg gttgaatctg ttaaatggac ccctcatgtt
    65221 caagcgtcgt tatgtaacat tgcttcccct ttttgtgttg cttgccgcct gtagcagcaa
    65281 gccaaaacct actgagactg aaacgaccac cggaacgccg tctggcggct tcctgcttga
    65341 gccgcagcac aatgtgatgc agatgggcgg cgatttcgct aataacccga atgcccagca
    65401 gttcatcgac aaaatggtga acaaacacgg tttcgatcgt cagcagttgc aggaaattct
    65461 ctcccaggcg aagcgtctgg attcggtact gcggctgatg gataaccagg caccaaccac
    65521 atcggtgaaa ccgccatcag gtccgaacgg cgcatggctg cgttatcgca aaaaatttat
    65581 tacgccggac aacgtgcaga acggtgtggt tttctggaat cagtatgaag atgcgttgaa
    65641 tcgcgcgtgg caggtgtatg gcgtaccgcc ggaaattatc gtcgggatta tcggcgttga
    65701 aacccgctgg gggcgcgtga tggggaaaac tcgcatcctc gatgcgctgg caacgctgtc
    65761 atttaactac ccacgccgcg cggagtattt ctctggcgag ctggaaacct tcctgctgat
    65821 ggcgcgcgac gagcaggacg atccgctcaa tctgaaaggt tcctttgccg gggcgatggg
    65881 ctacggacag tttatgccgt cgtcttacaa acaatatgcg gtagatttca gcggcgacgg
    65941 gcatatcaac ctgtgggatc cggttgatgc gatcggtagc gtggcgaact atttcaaagc
    66001 gcacggctgg gtgaaaggcg atcaggtcgc gataatggca aacggtcagg ctccaggctt
    66061 gccaaatggt ttcaaaacta agtacagcat ttcgcagctt gccgccgcag gtttaacgcc
    66121 acagcagccg ctgggcaacc atcaacaagc cagcctgctg cgtctggatg ttggcaccgg
    66181 ctaccagtac tggtacggtc tgccgaactt ctacaccatc acccgttaca accacagcac
    66241 ccattacgca atggcggtct ggcagttagg acaagccgtg gcgctggcgc gagtacagta
    66301 gctatttaca agaggggggc gtgagcttcc ctctggctaa tcagcttttt tctgaatcct
    66361 cctcgtaaaa ttgcaacgcc aacaccatct tcctgacgaa agtgctatct tgtccggcat
    66421 aaatttttga ctgacagagg ttgtgatgac tgacagtgaa ctgatgcagt taagcgaaca
    66481 ggttgggcag gcgctgaaag cccgtggcgc aaccgtaaca actgccgagt cttgtaccgg
    66541 tggttgggta gcgaaagtga ttaccgatat tgccggtagc tccgcctggt ttgaacgcgg
    66601 atttgtcacc tacagtaacg aagccaaagc gcagatgatc ggcgtacgcg aagagacgct
    66661 ggcgcagcat ggcgcggtga gtgaacccgt cgtggtggaa atggcgatag gtgcactgaa
    66721 agcggctcgt gctgattatg ccgtgtctat tagtggtatc gccgggccgg atggcggcag
    66781 tgaagagaag cctgtcggca ccgtctggtt tgcttttgcc actgcccgcg gtgaaggcat
    66841 tacccggcgg gaatgcttca gcggcgaccg tgatgcagtg cgtcgtcagg ctactgcgta
    66901 tgcattgcag accttgtggc aacaatttct acaaaacact tgatactgta tgagcataca
    66961 gtataattgc ttcaacagaa catattgact atccggtatt acccggcatg acaggagtaa
    67021 aaatggctat cgacgaaaac aaacagaaag cgttggcggc agcactgggc cagattgaga
    67081 aacaatttgg taaaggctcc atcatgcgcc tgggtgaaga ccgttccatg gatgtggaaa
    67141 ccatctctac cggttcgctt tcactggata tcgcgcttgg ggcaggtggt ctgccgatgg
    67201 gccgtatcgt cgaaatctac ggaccggaat cttccggtaa aaccacgctg acgctgcagg
    67261 tgatcgccgc agcgcagcgt gaaggtaaaa cctgtgcgtt tatcgatgct gaacacgcgc
    67321 tggacccaat ctacgcacgt aaactgggcg tcgatatcga taacctgctg tgctcccagc
    67381 cggacaccgg cgagcaggca ctggaaatct gtgacgccct ggcgcgttct ggcgcagtag
    67441 acgttatcgt cgttgactcc gtggcggcac tgacgccgaa agcggaaatc gaaggcgaaa
    67501 tcggcgactc tcacatgggc cttgcggcac gtatgatgag ccaggcgatg cgtaagctgg
    67561 cgggtaacct gaagcagtcc aacacgctgc tgatcttcat caaccagatc cgtatgaaaa
    67621 ttggtgtgat gttcggtaac ccggaaacca ctaccggtgg taacgcgctg aaattctacg
    67681 cctctgttcg tctcgacatc cgtcgtatcg gcgcggtgaa agagggcgaa aacgtggtgg
    67741 gtagcgaaac ccgcgtgaaa gtggtgaaga acaaaatcgc tgcaccgttt aaacaggctg
    67801 aatttcagat cctctacggc gaaggtatca acttctacgg cgaactggtt gacctgggcg
    67861 taaaagagaa gctgatcgag aaagcaggcg cgtggtacag ctacaaaggt gagaagatcg
    67921 gtcagggtaa agcgaatgca actgcctggc tgaaagataa cccggaaacc gcgaaagaga
    67981 tcgagaagaa agtacgtgag ttgctgctga gcaacccgaa ctcaacgccg gatttctctg
    68041 tagatgacag cgaaggcgta gcagaaacta acgaagattt ttaatcgttc gttattgatg
    68101 ataatagata atgtgtgcac ggacggtccc ctcgcccctt tggggagagg gttagggtga
    68161 ggggaacagg ccagcactgg cgtgaaaaat tactctcacc tcgatcctct aacgaattgg
    68221 gcctgcggga acattctgca tactcattga ctattaaatc tgtttatcag caaaacctgg
    68281 gtcgcatctg cggccctttt gcttttttaa gttgtaagga tatgccatga cagaatcaac
    68341 atcccgtcgc ccggcatatg ctcgcctgtt ggatcgtgcg gtacgcattc tggcggtgcg
    68401 cgatcacagt gagcaagaac tgcgacgtaa actcgcggca ccgattatgg gcaaaaatgg
    68461 cccagaagag attgatgcta cggcagaaga ttacgagcgc gttattgcct ggtgccatga
    68521 acatggctat ctcgatgaca gccgatttgt tgcgcgcttt atcgccagcc gtagccgcaa
    68581 aggttatgga cctgcacgta ttcgtcagga actgaatcag aaaggtattt cccgcgaagc
    68641 gacagaaaaa gcgatgcgtg aatgtgacat cgactggtgc gcactggcgc gcgatcaggc
    68701 gacgcgaaaa tatggtgaac ctttgccaac tgtcttttca gaaaaagtta agatccagcg
    68761 ttttctgctc tatcgtggct atctgatgga agatatccag gatatttggc gaaattttgc
    68821 cgactgaacg catacggtat tttacttccc agtcaagaaa acttatctta ttcccacttt
    68881 tcagttacca gcccggcggt taagacacgc tggagctggt ggcgatattt cgttagcttg
    68941 atttcaggat aattatgagc aagagcaccg ctgagatccg tcaggcgttt ctcgactttt
    69001 tccatagtaa gggacatcag gtagttgcca gcagctccct ggtaccccat aacgacccaa
    69061 ctttgttgtt taccaacgcc gggatgaacc agttcaagga tgtgttcctt gggctcgaca
    69121 agcgtaatta ttcccgcgct accacttccc aacgctgcgt gcgtgcgggt ggtaaacaca
    69181 acgacctgga aaacgtcggt tacaccgcgc gtcaccatac cttcttcgaa atgctgggca
    69241 acttcagctt cggcgactat ttcaaacacg atgccattca gtttgcatgg gaactgctga
    69301 ccagcgaaaa atggtttgcc ctgccgaaag agcgtctgtg ggttaccgtc tatgaaagcg
    69361 acgacgaagc ctacgaaatc tgggaaaaag aagtcgggat cccgcgcgaa cgtattattc
    69421 gcatcggcga taacaaaggt gcgccatacg catctgacaa cttctggcag atgggtgaca
    69481 ctggtccgtg cggcccgtgc accgaaatct tctacgatca cggcgaccac atttggggtg
    69541 gccctccggg aagtccggaa gaagacggcg accgctacat tgagatctgg aacatcgtct
    69601 tcatgcagtt caaccgccag gcagatggca cgatggaacc gctgccgaag ccgtctgtag
    69661 ataccggtat gggtctggag cgtattgctg cggtgctgca acacgttaac tctaactatg
    69721 acatcgacct gttccgcacg ttgatccagg cggtagcgaa agtcactggc gcgaccgatc
    69781 tgagcaataa atcgctgcgc gtaatcgctg accacattcg ttcttgtgcg ttcctgatcg
    69841 cggatggcgt aatgccgtcc aatgaaaacc gtggttatgt actgcgtcgt atcattcgtc
    69901 gcgcagtgcg tcacggcaat atgctcggcg cgaaagaaac cttcttctac aaactggttg
    69961 gtccgctgat cgacgttatg ggctctgcgg gtgaagacct gaaacgccag caggcgcagg
    70021 ttgagcaggt gctgaagact gaagaagagc agtttgcccg tactctggag cgcggtctgg
    70081 cgttgctgga tgaagagctg gcaaaacttt ctggtgatac gctggatggt gaaacggctt
    70141 tccgtctgta cgacacctat ggcttcccgg ttgacctgac ggctgatgtt tgtcgtgagc
    70201 gcaacatcaa agttgacgaa gctggttttg aagcagcaat ggaagagcag cgtcgtcgcg
    70261 cgcgcgaagc cagcggcttt ggtgccgatt acaacgcaat gatccgtgtt gatagtgcat
    70321 ctgaatttaa aggctatgac catctggaac tgaacggcaa agtgaccgcg ctgtttgttg
    70381 atggtaaagc ggttgatgcc atcaatgcag gccaggaagc tgtggtcgtg ctggatcaaa
    70441 cgccattcta tgcggaatcc ggcggtcagg ttggtgataa aggcgaactg aaaggcgcta
    70501 acttctcctt cgcggtggaa gatacgcaga aatacggcca ggcgattggt cacatcggta
    70561 aacttgctgc gggttctctg aaagtgggcg acgctgtgca ggctgatatt gatgaggctc
    70621 gtcgcgcccg tattcgtctg aatcactccg caacgcacct gatgcacgct gcgctgcgcc
    70681 aggttctggg gactcatgta tcgcagaaag gttcactggt taacgacaag gtgctgcgct
    70741 tcgacttctc acacaacgaa gcgatgaaac aggaagagat tcgtgcggtc gaagacctgg
    70801 tgaacgcaca gattcgtcgc aatttgccga tcgaaaccaa catcatggat ctcgaagcgg
    70861 cgaaagcgaa aggtgcgatg gcgctgttcg gcgagaagta tgatgagcgt gtacgcgtgc
    70921 tgagcatggg cgatttctcc accgagctgt gtggcggtac tcacgccagc cgcactggtg
    70981 atattggtct gttccgcatc atctctgaat cgggtactgc tgcaggcgtt cgtcgtatcg
    71041 aagcggtaac cggagaaggc gctatcacca ccgttcatgc agacagcgat cgcttaagcg
    71101 aagtcgcgca tctgctgaaa ggcgatagca ataatctggc ggataaagtg cgctcagtac
    71161 tgggacgtac gcgtcagtta gaaaaagaac tacaacagct taaagaacaa gctgccgcac
    71221 aggagagcgc aaatctttcc agtaaggcaa ttgatgttaa tggtgttaag ctgttggtta
    71281 gcgagcttag cggtgttgag ccgaaaatgt tgcgtaccat ggttgacgat ttaaaaaatc
    71341 agctggggtc gacaattatc gtgctggcaa cggtaaccga aggtaaggtt tctctgattg
    71401 caggcgtatc taaggacgtc acagatcgtg tgaaagcagg ggaactgatt ggtatggtcg
    71461 ctcagcaggt gggcggcaag ggtggtggac gtcctgacat ggcgcaagcc ggtggtacgg
    71521 atgctgcggc cttacctgca gcgttagcca gtgtgaaagg ctgggtcagc gcgaaattgc
    71581 aataatataa gcgtcaggca atgccgtgga ctcgcttcac ggcattcgca ttaacgctat
    71641 cgacaacgat aaagtcaggt tgaagttgtg tatatcggct aaacttaggt ttaacagaat
    71701 gtaatgccat gactgcttac atgtaatgtg tttgtcattg cttacttttt ggcgttatat
    71761 gatggataat gccgggatac agagagaccc gactctttta atctttcaag gagcaaagaa
    71821 tgctgattct gactcgtcga gttggtgaga ccctcatgat tggggatgag gtcaccgtga
    71881 cagttttagg ggtaaagggc aaccaggtac gtattggcgt aaatgccccg aaggaagttt
    71941 ctgttcaccg tgaagagatc taccagcgta tccaggctga aaaatcccag cagtccagtt
    72001 actaatcttt ccgcgtctca tctttatcgg tgagacgcac cctcaaaatt tctccttcac
    72061 tctatagtct tttcgcttta ctcccgttca ttcaacttaa gtctccattt ttttgcatta
    72121 ctgctatctg ccagacctcc gtttttctgt agataaaaca ctctttttga cgtttttaca
    72181 gactaattga gcgtgaagtg tgcaaacgat aaaagtgtgg gaaaaattgt ttgacttata
    72241 agtctcagaa agtaatatgt gcgccacgca gcgacgatga gcgataaaca agttcttcga
    72301 agcactcgta agaggcgtgt ggtgaggtgg ccgagaggct gaaggcgctc ccctgctaag
    72361 ggagtatgcg gtcaaaagct gcatccgggg ttcgaatccc cgcctcaccg ccat
//
LOCUS       DADKXM010000005        65606 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.005, whole genome shotgun sequence.
ACCESSION   DADKXM010000005 DADKXM010000000
VERSION     DADKXM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.005"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JQR05_000312"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JQR05_000312"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY5322564.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JQR05_000313"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JQR05_000313"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY5322565.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JQR05_000314"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JQR05_000314"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY5322566.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JQR05_000315"
     CDS             complement(3025..3480)
                     /locus_tag="JQR05_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY5322567.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JQR05_000316"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JQR05_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY5322568.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JQR05_000317"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JQR05_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY5322569.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JQR05_000318"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JQR05_000318"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY5322570.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="JQR05_000319"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="JQR05_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY5322571.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="JQR05_000320"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="JQR05_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY5322572.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKNGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASINVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="JQR05_000321"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="JQR05_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY5322573.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="JQR05_000322"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="JQR05_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY5322574.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="JQR05_000323"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="JQR05_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY5322575.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JQR05_000324"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JQR05_000324"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY5322576.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEESGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="JQR05_000325"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="JQR05_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY5322577.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="JQR05_000326"
     CDS             19305..19370
                     /locus_tag="JQR05_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322578.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="JQR05_000327"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="JQR05_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY5322579.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="JQR05_000328"
     CDS             complement(19754..20377)
                     /locus_tag="JQR05_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY5322580.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JQR05_000329"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JQR05_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY5322581.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDNAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JQR05_000330"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JQR05_000330"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY5322582.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="JQR05_000331"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="JQR05_000331"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY5322583.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRINLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="JQR05_000332"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="JQR05_000332"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY5322584.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /locus_tag="JQR05_000333"
                     /pseudo
     CDS             25216..26372
                     /locus_tag="JQR05_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26461..26847)
                     /locus_tag="JQR05_000334"
     CDS             complement(26461..26847)
                     /locus_tag="JQR05_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY5322585.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JQR05_000335"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JQR05_000335"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY5322586.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JQR05_000336"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JQR05_000336"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY5322587.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="JQR05_000337"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="JQR05_000337"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY5322588.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="JQR05_000338"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="JQR05_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY5322589.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="JQR05_000339"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="JQR05_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY5322590.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="JQR05_000340"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="JQR05_000340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY5322591.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPIATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="JQR05_000341"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="JQR05_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY5322592.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="JQR05_000342"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="JQR05_000342"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY5322593.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="JQR05_000343"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="JQR05_000343"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY5322594.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="JQR05_000344"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="JQR05_000344"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY5322595.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="JQR05_000345"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="JQR05_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY5322596.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMFVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="JQR05_000346"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="JQR05_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY5322597.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="JQR05_000347"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="JQR05_000347"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY5322598.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="JQR05_000348"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="JQR05_000348"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY5322599.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGTNAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="JQR05_000349"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="JQR05_000349"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY5322600.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="JQR05_000350"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="JQR05_000350"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY5322601.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="JQR05_000351"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="JQR05_000351"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY5322602.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="JQR05_000352"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="JQR05_000352"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY5322603.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="JQR05_000353"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="JQR05_000353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY5322604.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLTFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEQEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="JQR05_000354"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="JQR05_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY5322605.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="JQR05_000355"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="JQR05_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY5322606.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="JQR05_000356"
     CDS             53915..54304
                     /locus_tag="JQR05_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5322607.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="JQR05_000357"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="JQR05_000357"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY5322608.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="JQR05_000358"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="JQR05_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY5322609.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="JQR05_000359"
     CDS             complement(55963..56163)
                     /locus_tag="JQR05_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY5322610.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56873
                     /locus_tag="JQR05_000360"
                     /pseudo
     CDS             56329..56873
                     /locus_tag="JQR05_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="JQR05_000361"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="JQR05_000361"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY5322611.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="JQR05_000362"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="JQR05_000362"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY5322612.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="JQR05_000363"
     CDS             complement(58171..58995)
                     /locus_tag="JQR05_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY5322613.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQR05_000364"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQR05_000364"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY5322614.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYNKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQR05_000365"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQR05_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY5322615.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQR05_000366"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQR05_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY5322616.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQR05_000367"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQR05_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY5322617.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQR05_000368"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQR05_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY5322618.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQR05_000369"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQR05_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY5322619.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="JQR05_000370"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="JQR05_000370"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY5322620.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15511 a        16266 c        18077 g        15752 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccggacgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggaactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgct cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cactgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc taggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggccgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt atcattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg acagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatct aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgctcaa tctgtacaat ccggtgaata ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga agaatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tattaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgattct cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggctgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gattcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac gccgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatttgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggtgcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcgggccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gcattgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggataaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa acctgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccaatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagcgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accaaccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 gcatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacag ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgtggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta ataccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa tcgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggcaaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacaac ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgt ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga ccttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40861 ccaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcactaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgcgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctgacatt tttatttcct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtagcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcattaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagcaggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaacatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttgagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgctg gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gctaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcgcgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 atgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccgca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggcga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggctttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggccgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagttcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgataccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gttgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttccttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacgg acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagcag gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagaaca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtaatatacg
    65581 ccacctcgcg acagtgagct gagagc
//
LOCUS       DADKXM010000006        65019 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.006, whole genome shotgun sequence.
ACCESSION   DADKXM010000006 DADKXM010000000
VERSION     DADKXM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.006"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQR05_000371"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQR05_000371"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY5322621.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQR05_000372"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQR05_000372"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY5322622.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQR05_000373"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQR05_000373"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY5322623.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JQR05_000374"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JQR05_000374"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY5322624.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISDAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JQR05_000375"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JQR05_000375"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY5322625.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDTLGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCCDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JQR05_000376"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JQR05_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY5322626.1"
                     /translation="MHSTEVQAKPLFSWKTLGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JQR05_000377"
     CDS             9708..10559
                     /locus_tag="JQR05_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5322627.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JQR05_000378"
     CDS             complement(10546..10887)
                     /locus_tag="JQR05_000378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY5322628.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JQR05_000379"
     CDS             complement(10889..11767)
                     /locus_tag="JQR05_000379"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY5322629.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JQR05_000380"
     CDS             complement(11733..14030)
                     /locus_tag="JQR05_000380"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY5322630.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JQR05_000381"
     CDS             complement(14081..14401)
                     /locus_tag="JQR05_000381"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY5322631.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15480)
                     /locus_tag="JQR05_000382"
     CDS             complement(14416..15480)
                     /locus_tag="JQR05_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY5322632.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAL
                     IAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGALTNSL
                     TQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGI
                     CIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMV
                     GSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLAR
                     KNGSSTDEKEDDLDLDFEIN"
     gene            15789..18290
                     /gene="ptsP"
                     /locus_tag="JQR05_000383"
     CDS             15789..18290
                     /gene="ptsP"
                     /locus_tag="JQR05_000383"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY5322633.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSL
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18302..18964
                     /gene="fsa"
                     /locus_tag="JQR05_000384"
     CDS             18302..18964
                     /gene="fsa"
                     /locus_tag="JQR05_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY5322634.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18975..20077
                     /gene="gldA"
                     /locus_tag="JQR05_000385"
                     /pseudo
     CDS             18975..20077
                     /gene="gldA"
                     /locus_tag="JQR05_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20352..20969
                     /locus_tag="JQR05_000386"
                     /pseudo
     CDS             20352..20969
                     /locus_tag="JQR05_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20996..21934)
                     /gene="yijE"
                     /locus_tag="JQR05_000387"
     CDS             complement(20996..21934)
                     /gene="yijE"
                     /locus_tag="JQR05_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY5322635.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPRAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21995..24175)
                     /gene="katG"
                     /locus_tag="JQR05_000388"
     CDS             complement(21995..24175)
                     /gene="katG"
                     /locus_tag="JQR05_000388"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY5322636.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24504..25394)
                     /gene="metF"
                     /locus_tag="JQR05_000389"
     CDS             complement(24504..25394)
                     /gene="metF"
                     /locus_tag="JQR05_000389"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY5322637.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRSEMSYAICHTLGVRPGL"
     gene            complement(25743..28175)
                     /locus_tag="JQR05_000390"
     CDS             complement(25743..28175)
                     /locus_tag="JQR05_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY5322638.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVMTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28178..29338)
                     /gene="metB"
                     /locus_tag="JQR05_000391"
     CDS             complement(28178..29338)
                     /gene="metB"
                     /locus_tag="JQR05_000391"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY5322639.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29615..29932
                     /gene="metJ"
                     /locus_tag="JQR05_000392"
     CDS             29615..29932
                     /gene="metJ"
                     /locus_tag="JQR05_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY5322640.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30116..30724
                     /locus_tag="JQR05_000393"
     CDS             30116..30724
                     /locus_tag="JQR05_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY5322641.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30785..30997)
                     /gene="rpmE"
                     /locus_tag="JQR05_000394"
     CDS             complement(30785..30997)
                     /gene="rpmE"
                     /locus_tag="JQR05_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY5322642.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31200..33398
                     /gene="priA"
                     /locus_tag="JQR05_000395"
     CDS             31200..33398
                     /gene="priA"
                     /locus_tag="JQR05_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY5322643.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33554..34579
                     /gene="cytR"
                     /locus_tag="JQR05_000396"
     CDS             33554..34579
                     /gene="cytR"
                     /locus_tag="JQR05_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY5322644.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALCRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34671..35630
                     /gene="ftsN"
                     /locus_tag="JQR05_000397"
     CDS             34671..35630
                     /gene="ftsN"
                     /locus_tag="JQR05_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY5322645.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADTPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35723..36253
                     /gene="hslV"
                     /locus_tag="JQR05_000398"
     CDS             35723..36253
                     /gene="hslV"
                     /locus_tag="JQR05_000398"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY5322646.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36263..37594
                     /gene="hslU"
                     /locus_tag="JQR05_000399"
     CDS             36263..37594
                     /gene="hslU"
                     /locus_tag="JQR05_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY5322647.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKMKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37661..38587
                     /gene="menA"
                     /locus_tag="JQR05_000400"
     CDS             37661..38587
                     /gene="menA"
                     /locus_tag="JQR05_000400"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY5322648.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38680..39165
                     /gene="rraA"
                     /locus_tag="JQR05_000401"
     CDS             38680..39165
                     /gene="rraA"
                     /locus_tag="JQR05_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY5322649.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39250..39495)
                     /gene="zapB"
                     /locus_tag="JQR05_000402"
     CDS             complement(39250..39495)
                     /gene="zapB"
                     /locus_tag="JQR05_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY5322650.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39921..40766
                     /gene="glpF"
                     /locus_tag="JQR05_000403"
     CDS             39921..40766
                     /gene="glpF"
                     /locus_tag="JQR05_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY5322651.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            40789..42297
                     /gene="glpK"
                     /locus_tag="JQR05_000404"
     CDS             40789..42297
                     /gene="glpK"
                     /locus_tag="JQR05_000404"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY5322652.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKTVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42527..43537
                     /gene="glpX"
                     /locus_tag="JQR05_000405"
                     /pseudo
     CDS             42527..43537
                     /gene="glpX"
                     /locus_tag="JQR05_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            43634..44380
                     /gene="fpr"
                     /locus_tag="JQR05_000406"
     CDS             43634..44380
                     /gene="fpr"
                     /locus_tag="JQR05_000406"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY5322653.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44385..44813)
                     /gene="uspD"
                     /locus_tag="JQR05_000407"
     CDS             complement(44385..44813)
                     /gene="uspD"
                     /locus_tag="JQR05_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY5322654.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44840..45139)
                     /locus_tag="JQR05_000408"
     CDS             complement(44840..45139)
                     /locus_tag="JQR05_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY5322655.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45351..45791)
                     /locus_tag="JQR05_000409"
     CDS             complement(45351..45791)
                     /locus_tag="JQR05_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY5322656.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            45892..46491
                     /locus_tag="JQR05_000410"
     CDS             45892..46491
                     /locus_tag="JQR05_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY5322657.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46599..47366
                     /gene="tpiA"
                     /locus_tag="JQR05_000411"
     CDS             46599..47366
                     /gene="tpiA"
                     /locus_tag="JQR05_000411"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY5322658.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47421..48176)
                     /gene="cdh"
                     /locus_tag="JQR05_000412"
     CDS             complement(47421..48176)
                     /gene="cdh"
                     /locus_tag="JQR05_000412"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY5322659.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48283..49272)
                     /locus_tag="JQR05_000413"
     CDS             complement(48283..49272)
                     /locus_tag="JQR05_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5322660.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49592..50554)
                     /gene="pfkA"
                     /locus_tag="JQR05_000414"
     CDS             complement(49592..50554)
                     /gene="pfkA"
                     /locus_tag="JQR05_000414"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY5322661.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50735..51637)
                     /gene="fieF"
                     /locus_tag="JQR05_000415"
     CDS             complement(50735..51637)
                     /gene="fieF"
                     /locus_tag="JQR05_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY5322662.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKILAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51787..52287)
                     /gene="cpxP"
                     /locus_tag="JQR05_000416"
     CDS             complement(51787..52287)
                     /gene="cpxP"
                     /locus_tag="JQR05_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY5322663.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHKQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52437..53135
                     /gene="cpxR"
                     /locus_tag="JQR05_000417"
     CDS             52437..53135
                     /gene="cpxR"
                     /locus_tag="JQR05_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY5322664.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53132..54505
                     /gene="cpxA"
                     /locus_tag="JQR05_000418"
     CDS             53132..54505
                     /gene="cpxA"
                     /locus_tag="JQR05_000418"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY5322665.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54611..55285)
                     /gene="yiiM"
                     /locus_tag="JQR05_000419"
     CDS             complement(54611..55285)
                     /gene="yiiM"
                     /locus_tag="JQR05_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY5322666.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIALGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55434..56417)
                     /gene="kdgT"
                     /locus_tag="JQR05_000420"
     CDS             complement(55434..56417)
                     /gene="kdgT"
                     /locus_tag="JQR05_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY5322667.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVVTAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56677..57297)
                     /gene="sodA"
                     /locus_tag="JQR05_000421"
     CDS             complement(56677..57297)
                     /gene="sodA"
                     /locus_tag="JQR05_000421"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY5322668.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57582..58616
                     /gene="rhaT"
                     /locus_tag="JQR05_000422"
                     /pseudo
     CDS             57582..58616
                     /gene="rhaT"
                     /locus_tag="JQR05_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58613..59551)
                     /gene="rhaR"
                     /locus_tag="JQR05_000423"
     CDS             complement(58613..59551)
                     /gene="rhaR"
                     /locus_tag="JQR05_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY5322669.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(59535..60371)
                     /gene="rhaS"
                     /locus_tag="JQR05_000424"
     CDS             complement(59535..60371)
                     /gene="rhaS"
                     /locus_tag="JQR05_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY5322670.1"
                     /translation="MTVLHSVDFFPSCNASVAIELRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60659..62128
                     /gene="rhaB"
                     /locus_tag="JQR05_000425"
     CDS             60659..62128
                     /gene="rhaB"
                     /locus_tag="JQR05_000425"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY5322671.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62125..63384
                     /gene="rhaA"
                     /locus_tag="JQR05_000426"
     CDS             62125..63384
                     /gene="rhaA"
                     /locus_tag="JQR05_000426"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY5322672.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63626..64450
                     /gene="rhaD"
                     /locus_tag="JQR05_000427"
     CDS             63626..64450
                     /gene="rhaD"
                     /locus_tag="JQR05_000427"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY5322673.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRQDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFAVTPLASALAL"
     gene            64460..64774
                     /gene="rhaM"
                     /locus_tag="JQR05_000428"
     CDS             64460..64774
                     /gene="rhaM"
                     /locus_tag="JQR05_000428"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY5322674.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        15841 a        17522 c        16648 g        15008 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca acaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgttcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct gacgccatcc gtattcaggg ccagtcggga cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg tactgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatacgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgctgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt
     7861 tagctggaaa accctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacacc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    15241 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    15301 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    15361 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    15421 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15481 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15541 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15601 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagcatgccg caaaacggcg
    15661 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15721 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15781 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15841 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15901 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15961 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    16021 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    16081 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    16141 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    16201 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    16261 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    16321 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    16381 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    16441 gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagatacctg caagaacgtg
    16501 ccctagacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16561 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16621 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16681 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16741 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16801 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16861 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    16921 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16981 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    17041 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    17101 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    17161 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    17221 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    17281 tgaaaatcat gatcccgatg atctcctcac tggaagagat cttatgggtg aaagaaaaac
    17341 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    17401 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    17461 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17521 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17581 attacgccgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17641 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17701 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17761 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17821 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17881 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17941 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    18001 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    18061 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    18121 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    18181 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    18241 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    18301 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    18361 cccgattgcc ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatctg
    18421 ggaagtgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18481 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18541 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18601 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18661 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18721 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18781 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18841 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18901 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18961 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    19021 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    19081 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    19141 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    19201 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    19261 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    19321 ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    19381 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    19441 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19501 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19561 cccaggcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19621 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19681 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19741 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    19801 atttggtacg ctgacgcagc tggtactgga aaacgcgccg gtggaggaaa tcgaaaccgt
    19861 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    19921 agatgtcccg gcgaaaatgc ccattgttgc aaaagcatct tgtgcagaag gtgaaaccat
    19981 ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgcactgc tggtagctga
    20041 ccagtacggt cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt
    20101 ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta cgcaatctct
    20161 gcaatatatt gaatttgcgt gcttttgtag gccggataag gcgttcactc cgcatccggc
    20221 actctgtgcc agtaatctga aactcccacg ttgttcagga gttttttatc cgcttctgga
    20281 cattttctcc acagaacaat cgttagcccg gaaggtcgaa tctgtgactc ttattttcac
    20341 ggacgaacca gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc
    20401 actccttaaa atctcccgcc gttccaccaa ccgtggtaca aatctaggcc aatatcaacc
    20461 ttgccatcgc agacggtgcc agacagcaaa ttggcagcac gctattttac gatccggcgt
    20521 atatgcagct tacctatcct ggcggtgatg ttccgcaaga acgcggtgta tgttccgatg
    20581 tggtgatccg cgcattgcgc agccagaagg tcgatttgca gaaactggtt cacgaagata
    20641 tggcgaagaa ttttgctgag tacccgcaaa aatggaagtt aaagcgcccg gacagcaaca
    20701 tcgatcaccg tcgggtgcct aatctggaaa cctggtttag ccgccacaac aaaacacgcc
    20761 ccatcagtaa aaatgccggt gactatcaag cgggcgatat tgtctcctgg cgattagata
    20821 acgggctggc gcatattggg gtggtgtcag atggcttcgc ccgcgacggt acgccgctgg
    20881 tgatacataa cattggcgca ggcgcgcagg aagaagatgc gctgtttagc tggcggatgg
    20941 tggggcatta tcgttatttt gttaaataag agatcgcccc acatgaagaa aaaattcaga
    21001 tgctttttac actgacagct tcttttttct tacggctcac cagcgccagt gccagcacaa
    21061 tcagcacaat accgctacct tcaacggccc tcggattctc accgagcagc caccaggaaa
    21121 agagtacgcc acaaacggga acggccagtg tgcttaagct ggcaatactg gcaggcaagt
    21181 ttttcagcac aaacagccat aagctccacg ctagcgccgt cgccagaatc gcactgtagg
    21241 ccagcgccca gaacacggtg ggctgccagt caatttcacg ttgcggcacc agtaaagcga
    21301 ccacactcat caccagcgcc gcatacagca tctgccagga tgttaacgat aataaatcca
    21361 cgcgcggatg acgggcgtat aggcgtttag caacaatcgc gctcgccccc caactaacac
    21421 cggagaggat tgccagcatg gcacttttca tcgaagagaa atcgagttgc cacggctgca
    21481 acaccaaaaa taagccgaaa gcggcaatca gaatcgcgaa atattgccca cgtcgcaggc
    21541 gttcaccgag aaacaacgcg gcgaaaatca ccacccagaa cggcatggta tagctcagga
    21601 tcgccacctt ccccgcacct ccgctgacca acgcccactg cgccagacca accatcccgc
    21661 aggtttgtaa cagggcaatg gctaaggtgt atttaaacgg cgtcgggcgc attccgcgac
    21721 cacgtaataa aaggacgatg aataaaacga gagcgccgaa aatgcagcgt aaggcggtaa
    21781 agtcgaaggc accgatgtaa ctggtgactt gcttcatgaa aatccagcta taactccaga
    21841 taagtgtgag cacaaccagg ccactgattg ccagtgggtt gctctttcct gcggcagaca
    21901 tgagagatcc agtgtgtagt tttcctgtgg acactatacg ccggtaaaga aacttcagcg
    21961 actgcctgaa agacagtcgc tgaagaaggt cagattacag caggtcgaaa cggtcgaggt
    22021 tcatcacttt cacccatgcc gctacgaagt ctttaacaaa cttctcgtgg gcatcgctgc
    22081 tggcgtaaac ttccgctact gcacgcagga cagagttaga accaaatacc agatccgcac
    22141 ggctggctgt gtatttcact tcgccggttt cgcggtcacg tccttcgaac agctctttcg
    22201 attcgtcagt cgctttccac tcgtaacgca tatccagcaa gttcacgaag aagtcattgc
    22261 tcaatacgcc aacgcggtca gtgaagacgc cgtttttgct gccatcgaag ttggcaccca
    22321 gtacacgcat cccgcccacc agcgcagtca tttccggcgc ggtcagcgtc agttgctgtg
    22381 ctttgtcgat cagcagtgac tcggtggtgg aaacgtccag acgagcgcga tagttacgga
    22441 aaccgtcagc aattggctcc agcagctcaa acatctcaat gtcagtctga tcctgacgcg
    22501 catcaacgcg acccggcgca aacggtacat gaatgctcaa acctgcggcg cttgcggctt
    22561 tctcaacacc aaccacacca gccagcacga tgatatccgc cagcgaggct ttaccagact
    22621 ctttctggat tttctccaga acaggcagag cacgaacggc tgcggcgttc acatcccagt
    22681 cgcgctgcgg cattaatgcc agacgcgcac cgttggcacc accgcgtttg tcgccaccac
    22741 ggaaggtaga agcagatgcc caggccaccg ataccagctc actaacagac agaccagaat
    22801 ccgcaatcgc gaatttcaga tcgataatgt cctgctcggt cgggttgtag atcggctgcg
    22861 gcagcggatc ttgccagatc agatcttctt tcggcacttc cggcccgatg tagcgagatt
    22921 tcggccccat atccctgtgc gtcagtttga accaggcacg ggcaaaggct tcgttgaacg
    22981 cctgcggatc gttgaggaaa cgacgagaga tcttctcgaa ctcaggatca aaacgcagcg
    23041 tcaggtcggt caccagcatt gtcggtttac gtttcttcga cggatcgaac ggatccggga
    23101 taatttccgg tgcgtctacc gcttcgaact ggattgctcc agccggactg cgggtctgta
    23161 cccactcata cttgaacaga ttctcgaaga aatagttgct ccactgggtc ggcgtctggg
    23221 tccagactac ttccagacca gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc
    23281 tcgcccagcc taaaccttgt tcttcaatcg gtgcagcttc tggatcagga cctacgtttg
    23341 atgtcggacc ggcaccgtgg gttttaccca gcgtatgacc acccgcaatc agcgccacgg
    23401 tttcttcgtc gttcatgccc atgttgccga aggtcgcgcg gatagctgct gccgcagaaa
    23461 gcggttcgcc gctgtgatcc gggccttccg ggttaacgta aatcaggccc atctcggttg
    23521 cacccagcgg tgctttcgcc agcgcttccg gatgacggtg agtcagccag gctttttcat
    23581 caccccagtt aacatccaga tccggttccc agacgtcttc acgaccggca ccaaaaccga
    23641 aggtacggaa gccggagttt tccagcgcca cgttacctgc gaggataaac aggtcggccc
    23701 aggagatttt ctgaccatat ttctgtttga ttggccacaa caggcgacgc gctttatcga
    23761 ggcttacgtt atccggccag gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc
    23821 cgccgcgtcc atcgattgaa cggtaagtac ctgcgccgtg ccaagccata cgaataaaca
    23881 gaccggcgta gctgccccag tcggctggcc accacggctg agattctgtc agcagggctt
    23941 tcagatcttt tttcaggccg tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt
    24001 cctcacccag cgggttagaa cgattagaat gttggtttaa caggtcaaca cggagttgat
    24061 ttggccacca gtcgcgagtg gttgtgcccg cccccgcgct ctggtcgtga ccgccctgat
    24121 ggaacgggca tttgccagtg gctgtggtgt tatggatatc gtctgacgtg ctcatcaatg
    24181 tgctcccctc tacagtgtta ccgttacgat acacagcgtt agagagaagt tataattgaa
    24241 ttaatccacg gatgcgatag ttgagatctt acatattgtt ggttaaagag atgtagatca
    24301 aattgatcgt aattaagctt ttttgattat gtccgaattc ggacaaatgc tttataaaaa
    24361 agggttttag cttcatatcc ttcaagtaat gagggagaaa acaaggctga caaccttatt
    24421 ttcccgccct catttcgagg cagcattttg tgctctgttt aaaatttgtg atcactgtgt
    24481 gattttcaca aaagccacac tatttataaa ccaggtcgaa cacccagcgt atggcaaatc
    24541 gcgtaactca tttcagagcg gttaagcgta tagaagtgga aatctttcac tccttcacgg
    24601 cttaaaatct tcaccatatc catggcaata ttcgcgccca ccagtttgcg ggtttcggca
    24661 tcgtcatcca gcccgtcaaa catttgcgcc atccacgccg gaatacgcac gttggtcatg
    24721 tcggcaaatt tcttcgcctg tttaaagtta gataccggca aaatgcccgg gatgatttcc
    24781 acatcaatgc ccgccgatac acagcggtca cgaaaacgca ggtagctttc gacatcgaag
    24841 aagaactgag taatcgcgcg gttggctccg gcatccactt tgcgtttcag attaagcaaa
    24901 tccgcctgag cgctttttgc ttccgggtga acttccggat acgccgccac ggatatatcg
    24961 aaatctgcca cttcttttaa cagcgtcacc aggtcagaag catacatttc tggcttacca
    25021 cttcccggcg gcagatcgcc acgcagtgcc acgatatgac gaataccgtt attccagtag
    25081 tcgcgtgcaa tggtgcgcag ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc
    25141 gcttccagac cagtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg
    25201 ccggagttcg cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg
    25261 atggagttcc acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa
    25321 acgttaatct gcccctggac ttctgccagg ctctgattca gggcatcccg ctggctggcg
    25381 tgaaaaaagc tcatacctta cctcatcaat cgcttgtagt tatatgttgt gttgtgcaca
    25441 tctatccatt tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa
    25501 aaatgaccaa ttatggtgag gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga
    25561 aaaagaaaat ccgatatgca aagcaaaccg gactgcaaat ataaactaac catgtactta
    25621 ttgcttcttg ccggattcgt cgtaaacgct tcatccggcc taactctatg ataattcaat
    25681 atatttcatt agaaaccata gacccgaaaa catgagtacc gggcattatt aaatttctga
    25741 aattacaaca actgtgccag ccggttgata tccgactgaa tcgccccggc ggtgacgtcg
    25801 cgcccagcgc caggcccacg gatcaccagc ggattatcgc ggtaccagcg gctttcgatg
    25861 gcaaagacgt tatcgcatgg cagcagtgat gccagcggat ggtcttcacg caccgcctcc
    25921 acgcccacac gcgctttgcc gttggcatcg aaacgcgcca cgtagcgcag caccagcccc
    25981 atttcgcggg ccgcttccag ccgttgcacc atctgctcgt taagttcatc gccattttca
    26041 aagaaatggt cgatgctgcc gccttcgcaa tgagcaggca ccagcgattc cacacgtacc
    26101 tggtccggtt caatgttgta acccgcttca cgcgccagaa tcaccagctt gcgcattacg
    26161 tctttgccgg agaggtcgtc acgcggatca ggctcggtta agccctgctg ccacgcctga
    26221 tccaccagct cggtaaacgg cacgctaccg tcgaattgca ggaacagcca ggagagcgtg
    26281 ccggagaaga tcccactgat cgacaaaatg gtatcgccgc tgtcgatcag atcgcgcacg
    26341 gtatggttga tcggcaagcc cgcaccaacg gtggcgttgt atagccagtg acgcccggtt
    26401 ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt cgcttgctcc cgccagtttg
    26461 ttggcactga tgacgtggaa accgtggctg gcgaaatcga gatactgatc agcaagctgc
    26521 tggctggcgg taacgtccaa caccactaaa tcatcatacg gatgggcgcg catccacagg
    26581 aaaagcgact cttcatcctg ctcgaccgct tcatcgttga agaaggctaa cgcgcggctg
    26641 gcgtccagcc cgtcataact caacagactg cggcggctgt ccaccacgcc tgccagcaca
    26701 aattcaaagc cggtacgcgc cgaaagcgtg ctctgctcgc gggcgaacag ttccagccag
    26761 cgagagccga tattgccttt gccgaacaat accaggccga tgcgtttttc tgcgcggaaa
    26821 acggactgat gcagcccctg aatcaggctt tcggtcgggc cggtgcgcag tactgccacc
    26881 agactgatgc cgtcatcgga ctgccacgta aactcgaccg gctggccttt cagttgctgc
    26941 cagaagcggt ggcaatgcag cgggttacgg gtgacacccg ccccgaccat cgccaccagc
    27001 gccagcccct gacgcaggcg cagttctcca ggtaatcccg cttcgtcgag gattttcagc
    27061 gcactgtcgg ccacttctga ggtgtagcaa aattgcagca actggcgatc gttatgcacg
    27121 ccaaccgcca gtggacgcac ctgggcacgt ttcaggatct ggtcgatctc tttatgcgcc
    27181 agtttgaaat cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg
    27241 gtgacaatac gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga
    27301 tccggcgtgt agctacagcg cagctgcagg tcgatttcgc tgccagaaac tggctgtaaa
    27361 gtacgggcgt gaagaacggg agccgccaga cgcgccagtt cgctggcctc atccagacgc
    27421 agcaacggca gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg
    27481 acgtcgctcc agatggttac acgagaaaca cccgccagcg caccgatttg tgtcgcggaa
    27541 tagtcggaac cgttacgccc cagcaacacc gtttcaccgg cgttgttacg gctgataaac
    27601 ccggtcatca ccagacgttt gctcggatgt tgtaccagta gctgttgcag caacgggtaa
    27661 gaaagccctt catcaacctg cggttgcgca gcgcgttcag cgcgtaaaaa ctcacgggca
    27721 tcaagccagg ccgctggcag cccttgttga ttaagtaccg cagacatcag gcgcgccgac
    27781 cacacttcac cgtggcccac cacttccgca tacaccgcat cgttaatacc actgtcgagc
    27841 aacgccgcca ggcgctcaag atcgctgaca aaagcgctaa tgaggctatc ggcttcttca
    27901 gcgggtagca gaccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga
    27961 tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg
    28021 ctaccggcgg cggaaaccac catcatatcg tcaggctgag agaactccgc cataatgccc
    28081 gcgacacgca aataacactt cacatcagcc agactactgc caccaaattt atgcagctga
    28141 cgacctttcg cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga
    28201 aaccattttc caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca
    28261 gcagcgtctc ggagatcccg gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca
    28321 tggttgcggc gtgagagatt aaactttcca ctccccctaa tgattccgcc agcgtaaaca
    28381 acgacagccc gcccaggaaa cgacacagcg tctgctcatc gccatccagt tcaaaactca
    28441 acattgcgcc aaaacctttt tgctgacgcg cggcaatttc atgcccctga ttttccggca
    28501 gcgacgggtg atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct
    28561 gcgcgttgcg ctgcgccagc tccatacgcg gcaccagcgt tcgcaacccg cgtagcagca
    28621 gatagctgtc aaacgcgccg cccgtcacgc caatattgtt tgcccaccag gcaagttcag
    28681 tgacaacgtc cgggtcttta gcaatcacca cgccagccac cacgtcagag tgaccgttca
    28741 gatatttcgt acatgaatgc aacaccagat cggcacccaa tgccagcgga ttttgtaatg
    28801 ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc
    28861 agattttcgc aatatccacg acgcgtaaca atggattact tgggctttct accagtacca
    28921 gtttgggttt ttctgccagc gctgcccgta atgcatgttc atcgccttga tcaacaaaca
    28981 acacgcgata gcaaccgcgt ttcgccagac tgtcgaacag gcgatagcta ccgccgtagc
    29041 agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt accaggtgaa
    29101 tcgcggacat gccggtatta gtaagtactg cgccagcacc gccttccagt tctgccagcg
    29161 cacgctgaac cacatcgcgc gttgggttgc cgcgacgcga gtaatcatgc gcgcgcggtt
    29221 cattaaatcc ggtaaagtta taggtgctgg aaagatggat gggagggaca acgcaaccat
    29281 actgttcgtc gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt
    29341 gatgaagttt cctgggcttt gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa
    29401 tggacgtcaa tacatctgga catctaaact tctttgcgta tagattgagc aaatcccaaa
    29461 tagccgttaa aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa
    29521 cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc acataaattg tggggtctgg
    29581 tctcaattta ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat
    29641 cagcccatac gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat
    29701 tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct
    29761 gcgtcacgct accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca
    29821 acctttgcca gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc
    29881 aaaagagatc atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcggaaa
    29941 agagcggcgc ggagtggaat cgcctgatgc actacgctta tcaggcctac gtcatattgc
    30001 aatttattga atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat
    30061 aaacaacgag cacgttgtct gcgacccacc gctttttata catggacgtt taactatgaa
    30121 aaacaggctg ctgatcctca gcctgctggt ttctgtacct gcctttgcct ggcagccgca
    30181 aactggcgac atcatctttc aaatctctcg ctcatcgcaa agtaaagcga tccaactggc
    30241 aacccattcc gattacagcc acaccggtat gctggtgatg cgtaacaaaa agccctacgt
    30301 ttttgaagcg gtcggcccgg tgaaatacac cccgctcaag cagtggattg cccatggtga
    30361 aaagggcaac tacgttgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa
    30421 actgacgcaa acggcaaaac gttatctcgg taaaccgtat gatttcagct tctcatggag
    30481 cgacgatcgc cagtactgtt cggaagtggt gtggaaggtt taccagaacg cgctgggaat
    30541 gcgcgtgggc gaacagcaga agttgaaaga gtttgatctc agcaacccgc tggtccaggc
    30601 gaagctcaaa gaacgctacg gtaaaaatat tccgcttgag gaaacggtag tttcaccaca
    30661 agccgttttc gacgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg
    30721 gtgatacgac caaaattcag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc
    30781 ggtcttattt gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg
    30841 tagcaacatc acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt
    30901 tcaggtcatg accaacggtg gagcggattt tcattacgtt accgcaagag cagctagcag
    30961 taatttcttc gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg
    31021 cgtcgctctt ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac
    31081 gatcatttcg tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc
    31141 cgcactcttc tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta
    31201 tgcccgttgc ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc
    31261 cagaaggcat gacggttaaa gctgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg
    31321 agcgcatcgg gattgtggta tcagttagcg atgccagcga actgccgctc actgagctaa
    31381 aagcggtagt cgaagtgctg gatagtgagc cggtgtttac tcactccgtc tggcgattgc
    31441 tgctatgggc ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc
    31501 cgattttact acgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca
    31561 ctgaacaagg tcaggcggtg gatctgaaca gcctgaaacg ctcacccaag caacaacagg
    31621 cgctggcagc gttacggcaa ggcaaaatct ggcgcgacca ggttgccacg ctcgaattta
    31681 atgatgccgc gttgcaggcg ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac
    31741 cagagtttag cgactggcga acgaactatg ccgtttctgg tgagcggttg cgattgaata
    31801 ccgaacaggc caccgccgtt ggcgcaattc atagcgcggc agatactttt tctgcctggc
    31861 tgctggcggg cgttaccggt tccggtaaaa cggaggttta tctcagtgta ctggaaaacg
    31921 tgctcgctca gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa
    31981 ctatcgcccg ttttcgtgaa cggtttaatg cgcccgtgga agttctgcat tccggtctga
    32041 acgacagcga gcgtctttcg gcgtggctga aagcgaaaaa tggcgaggcg gcgattgtga
    32101 tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt gtcattgacg
    32161 aagagcacga cagttcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg
    32221 cggtgtatcg tgcgcatagc gagcaaatcc cgattattct tggctccgca acgcccgcgc
    32281 tggaaacgtt gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgccggg
    32341 cggggaatgc gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg
    32401 caggtctggc tccggcgtta atcacccgta tgcgccagca tttacaggcc aacaatcagg
    32461 ttatcctctt tcttaaccgc cgtggctttg cgcctgcgct actgtgccac gactgcggct
    32521 ggattgccga atgcccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt
    32581 tgcgctgtca ccactgtgac agtcagcgtc cggtgccgcg ccagtgccct tcctgcggtt
    32641 ccacgcacct ggtacccgtg gggctgggca ccgaacagct tgaacagacg cttgcgccgc
    32701 tgttccctga tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggggcgc
    32761 tggaacagca actggcggaa gtacatcgcg gcggtgcgag gattttgatc ggcacacaaa
    32821 tgctggcgaa aggtcaccac tttccggatg tgacgctggt ggcgttactg gatgtggacg
    32881 gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt cgctcagctt tacacccagg
    32941 tcgctggtcg tgccgggcgt gcgggtaaac aaggcgaagt ggtgctgcaa acgcaccatc
    33001 cggaacatcc tctgttgcaa acgttgctct ataaaggcta cgacgccttt gccgaacagg
    33061 cgctggctga gcgacggatg atgcaactgc cgccatggac cagccatgtg attgtgcgtg
    33121 cggaagatca taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc
    33181 tctccagccc gctggcagac gacaaactgt gggttctcgg tccggttccg gcactggcac
    33241 ctaaacgtgg cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct
    33301 tgcaacacat cattagtggt acgctggcgc tcatcaatac aataccggat tcccgcaagg
    33361 tgaaatgggt actggatgtt gatccgattg agggttaaac cgcttacgat gcgaggcgga
    33421 tcgaaaaatt caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct
    33481 atctgctaaa aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag
    33541 gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc
    33601 cctcaaggca aaagtctcta cagcgactgt ctcccgagca ttaatgaatc ccgataaagt
    33661 ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt atttaccgca
    33721 gcctatgggg cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga
    33781 tatctgcgat cccttcttta gcgaaattat tcgcggtatc gaagttacgg cggcaaatca
    33841 cggatatctg gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat
    33901 cgatttgatc atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt
    33961 tgatgccagc attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaatttgc
    34021 accggaactg gagctgccta ccgttcatat cgacaatctg accgccgcat ttgatgcagt
    34081 aaattattta tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat
    34141 gccactgtgt cactaccgcc tgcaaggcta tgttcaggcg ctgtgtcgct gcggaattat
    34201 ggttgatcca caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcaat
    34261 gcaacagctg cttgatcttc cacaaccgcc tactgcagtc ttctgccata gcgatgtgat
    34321 ggcgctcggc gcactttctc aggcaaaacg ccaggggctg aaagtcccgg aagacctttc
    34381 cataatcggt tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat
    34441 cgcgcagccg cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca
    34501 ggggcaacac gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc
    34561 aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc
    34621 gcttaagtaa catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac
    34681 gagattatgt acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga
    34741 aaaagcaacg aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgttc
    34801 ttgtgacctt tatcggaggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga
    34861 cgctgcaaag ccagaaagtg accggaaacg gactaccacc aaaaccagaa gaacgctggc
    34921 gctacattaa agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg
    34981 ccggtggtga agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac
    35041 aaatgcaagc tgatatgcgc cagcagccaa cgcagctggt tgaggttccg tggaatgaac
    35101 agacgccgga acagcgtcag caaacgctac aacgccagcg tcaggcgcag caattagcgg
    35161 agcagcaacg cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc
    35221 gtacgtcgca agccgcgccg gtacaggcgc aaccgcgcca gtctaaacca gcttctactc
    35281 agcagccgta ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc
    35341 agcaagctgc gccggttgct cgtgccgctg acacgccaaa accgacggcg gagaaaaaag
    35401 acgaacgccg ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg
    35461 tacgtgctca gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga
    35521 atcgtgtggt cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt
    35581 tgaagatggc gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa
    35641 atccccccca tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta
    35701 ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt
    35761 ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa
    35821 aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga
    35881 tgcttttacg ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt
    35941 taaagccgcc gttgagctgg caaaagactg gcgtaccgat cgcatgctgc gcaaacttga
    36001 agcactgctg gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt
    36061 ggtgcagcca gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc
    36121 ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt gaaattgctg aaaaggcgtt
    36181 ggatattgca ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag
    36241 ctacaaagcg taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact
    36301 ggataagcac atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa
    36361 ccgctggcgt cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat
    36421 cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct
    36481 ggcgaatgcg ccgttcatca aagttgaagc gaccaaattt accgaagtgg gctacgtcgg
    36541 taaggaagtt gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt
    36601 ccaggctatc gagaaaaacc gttatcgcgc tgaagagctg gcagaagaac gtattctcga
    36661 cgtgctgatc ccacctgcta aaaacaactg gggacagact gaacagcagc aggaaccttc
    36721 cgctgctcgt caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat
    36781 cgagatcgat cttgccgcag caccgatggg cgttgaaatt atggctcctc cgggcatgga
    36841 agagatgacc agccagctgc agtccatgtt ccagaacctg ggcggccaga agcaaaaagc
    36901 gcgtaagatg aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact
    36961 ggtgaacccg gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt
    37021 gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc
    37081 tcgtgaaggc gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg tttccaccaa
    37141 acacgggatg gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc
    37201 gaaaccgtct gacctgatcc cggaactgca aggtcggctg ccaatccgcg ttgaactgca
    37261 ggcactgacc accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt
    37321 gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat
    37381 taaacgcatc gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg
    37441 tcgtttacac actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt
    37501 aagcggtcaa aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt
    37561 ggcagatgaa gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt
    37621 ttgatggggc tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag
    37681 ccgaactcag gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc
    37741 aattatcgtc gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct
    37801 gctggcacta attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg
    37861 cgatgcggta aaaggcagcg ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa
    37921 aggggtcatt acccagcaaa agatgaaacg ggcgctcatt attaccgtcg tgctcatctg
    37981 tctctccggg ctggcactgg ttgcagtggc atgccatacg ctggccgatt ttgtcggttt
    38041 cctgattctt ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc
    38101 ttatggttat atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttgagtgt
    38161 catggggagc tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac
    38221 cgcatgcggc ctgctggcaa cggcagtact gaatattaat aacctgcgtg atatcaatag
    38281 cgaccgcgaa aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg
    38341 ttatcatgcc tgcctgctga tgggctcgct ggtgtgtctg gcgctgttta atctcttttc
    38401 gctgcatagc ctgtggggct ggctgttcct gctggcggca ccattactgg tgaagcaagc
    38461 ccgttatgtg atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg aacgtactgt
    38521 caagggagcg ttactgacta acctgctgtt tgttttaggg atattcctaa gccagtgggc
    38581 agcataactg acaaatatca attaacaatt gatgattttg ccaacagccc acatagcgcg
    38641 atatactgaa aattctcgca gtaactgaat gttaagccta tgaaatacga tacttccgag
    38701 ctttgtgaca tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc
    38761 ggacgggcgt cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg
    38821 ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt
    38881 tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg
    38941 gaaggtctgg tcatttacgg cgcggtgcgt caggtagatg acctggaaga gttggatatc
    39001 ggcatccagg cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc
    39061 gatgtccgcg tcaattttgg cggtgtcacc ttcttctccg gcgaccatct ttatgccgac
    39121 aataccggga ttattctttc agaagatccg ctggatattg aatgataaga aaggcaccgc
    39181 aaggtgcctt ttttctgcgt tacctgttgg cctacacagt aaagaaatta cgcgaaagat
    39241 gaagcgtaat cagacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc
    39301 gttctgctgt tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc
    39361 attttgaact tcctgcgaca atgagttgtt tttttctttc agctcttcga tttccatctg
    39421 caacagagtg atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc
    39481 taatgacatt gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc
    39541 ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga
    39601 agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat
    39661 ccttctccta gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat
    39721 gatctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt
    39781 ttatgacgaa gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa
    39841 gttaacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat
    39901 taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg
    39961 aattcctcgg taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag
    40021 tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa
    40081 tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg
    40141 cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag
    40201 ttgccggcgc tttctgcgct gcggctttag tttacgggct ttactacaat ttatttttcg
    40261 acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggctggca
    40321 ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg
    40381 tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac
    40441 cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta
    40501 tgggcccatt gacaggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg
    40561 cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc
    40621 tggtgccgct tttcggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac
    40681 tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggaa accacaactc
    40741 cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa
    40801 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    40861 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt
    40921 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    40981 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    41041 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    41101 cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat
    41161 atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg
    41221 atcctcgacc atgtggaagg ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt
    41281 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    41341 accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg
    41401 ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    41461 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    41521 ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac
    41581 acctatggca ctggctgctt tatgctgatg aacactggcg agaaaacggt gaaatcagaa
    41641 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa
    41701 ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt
    41761 aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat
    41821 gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg
    41881 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    41941 attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg
    42001 cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc
    42061 gatattctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg
    42121 gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct gcaagagaaa
    42181 gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac
    42241 gcaggctgga aaaaagcggt caaacgcgcg atggcgtggg aagaacacga cgagtaatgt
    42301 caggcacctg aatcgattgc ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc
    42361 aggcctacga gccaatgcca ttttgtgtag gcgggataag gcgttcacgc cgcatccggc
    42421 atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa tcccaccctt
    42481 cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact
    42541 tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg
    42601 acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa
    42661 ctaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat
    42721 gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga
    42781 tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc
    42841 agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt
    42901 cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa
    42961 tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc tggctaaacc
    43021 acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc
    43081 ggacggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct
    43141 gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga
    43201 tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa
    43261 tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa
    43321 agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat
    43381 taccaaaggc gatctgctgg aaggtattag ccgcaaaggc aatatcgcga ctaccgaaac
    43441 gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga
    43501 tcgcaaagac ccggaaatgc aggtgcacat tctctgagtg atttgatcga ttgagccttc
    43561 cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa
    43621 acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac
    43681 cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt
    43741 taccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa
    43801 ctcgcccgat aatcccgatc tggagtttta cctagtcact gtccccgatg gcaaattaag
    43861 cccacgattg gcggcactga aaccgggcga tgaagtgcag gtggttagcg aagcagctgg
    43921 cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg caaccggtac
    43981 agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa
    44041 tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca
    44101 agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac
    44161 ggcagcgggg tcgctcacgg gacggatacc ggcattaatt gaaagtgggg aactggaaag
    44221 cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca
    44281 gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg
    44341 tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac
    44401 ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc cggcatcaaa
    44461 cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata
    44521 atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat
    44581 tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga
    44641 atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata
    44701 tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt attcaccagt
    44761 aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac
    44821 tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct
    44881 cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa
    44941 tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca
    45001 gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg
    45061 tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt
    45121 tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt
    45181 aagaataatg cgccgttaag ggaagaataa acaagtaatg atcttatgga ttaccatttt
    45241 ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc
    45301 gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt
    45361 gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct
    45421 tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca
    45481 aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagcagc
    45541 atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc
    45601 ttaacagtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc cgcggtctga
    45661 atatcgagta gattcttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg
    45721 cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc
    45781 tgtatggtca tgtaagttcc ttgatggttg tcttttcctg gattctaccc ttttgacaag
    45841 ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca
    45901 gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca
    45961 caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat
    46021 ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct gccactgaac
    46081 gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa
    46141 attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc
    46201 attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct
    46261 caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc
    46321 cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca
    46381 gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt
    46441 gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca
    46501 aagcctttgt gccgatgaat ctctatactg cttcacagac ctgctgccct gcggggcagc
    46561 catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg
    46621 gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta
    46681 aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg
    46741 atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc
    46801 tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa gacatcggcg
    46861 ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac
    46921 tgattgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg
    46981 gtgaaaccga agctgaaaac gaagcgggaa aaactgaaga agtttgcgca cgtcagatcg
    47041 acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac
    47101 ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca
    47161 aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc
    47221 agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg
    47281 acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg atcgttaaag
    47341 ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt
    47401 actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg gatttcctcg
    47461 gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaacac aaacgaatta
    47521 tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggcact
    47581 tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc
    47641 gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc
    47701 gccagatttt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga
    47761 aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga
    47821 accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag
    47881 ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc
    47941 atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc ggcattgggt
    48001 ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca
    48061 aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg
    48121 gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt
    48181 ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc
    48241 ggtcgcgaga aaccaccggg tttgataatt atcctaggga aatcagcgtt tgctgatctg
    48301 atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc
    48361 ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc
    48421 agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag
    48481 atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac
    48541 caccgacacc gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc
    48601 tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc
    48661 aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac
    48721 gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg
    48781 cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt
    48841 aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg
    48901 attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag
    48961 acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca cgtcataggc
    49021 cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc
    49081 tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt tccagtgggc
    49141 gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag
    49201 aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc
    49261 ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag
    49321 gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg
    49381 gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc
    49441 ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga
    49501 attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca
    49561 ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc
    49621 agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca
    49681 gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag
    49741 cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct
    49801 ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg
    49861 ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt
    49921 tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa
    49981 cgacaacgaa ttcacagcca ccggcaatgg ccgcagccaa ggtcagatcg ccacaataac
    50041 ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt
    50101 cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt
    50161 tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac
    50221 gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt
    50281 ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg
    50341 cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct
    50401 gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt acttccagac
    50461 cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac
    50521 cgccgcttgt caacacacct attttcttaa tcatgactac ctctgaactt tggaatgcaa
    50581 aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat
    50641 agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata
    50701 cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct
    50761 ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc ggaaaacgcc
    50821 gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt
    50881 cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat
    50941 cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttctt
    51001 catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc
    51061 tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt
    51121 accaagacaa ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat
    51181 gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac cgctgaaacg
    51241 agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat
    51301 ctgtcatcgg tgttggagat accagatgtt gaatacccgt caaaaacagg aatagtgccg
    51361 aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa
    51421 acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg
    51481 acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat
    51541 accaccatgc caaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg
    51601 ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac
    51661 gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa
    51721 aaaaaaaacc ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag
    51781 ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg
    51841 ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc
    51901 ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg
    51961 acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa
    52021 attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg
    52081 tttgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact
    52141 tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca
    52201 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc
    52261 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    52321 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    52381 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    52441 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    52501 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    52561 acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca
    52621 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    52681 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    52741 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    52801 agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtgctga
    52861 atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt
    52921 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa
    52981 gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat atgcacattt
    53041 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc
    53101 gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    53161 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    53221 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcaggggc tgatgattga
    53281 gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    53341 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    53401 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    53461 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    53521 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    53581 cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt
    53641 caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    53701 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    53761 actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac
    53821 cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct
    53881 gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga
    53941 aagcaaggaa ctcgagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga
    54001 tctgttggtg atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc
    54061 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    54121 gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaatc caaacgccct
    54181 ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga
    54241 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    54301 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg
    54361 cgatcgtgaa tctggcggta caggattggg gctggcgatt gttgaaaccg ccattcagca
    54421 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    54481 gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc
    54541 cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac
    54601 actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc
    54661 ggcgcttttg catcgtccgc gtccagcttt tcgataaccc ggcggcggag agtaaacggt
    54721 gatactgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca
    54781 catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc cctagcgcta
    54841 tcacgctata caaccacccc accttacctg tgttttgcat caactgcgct atatcgctga
    54901 tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg
    54961 cctctcccca gcggaaaata tcgcccatat gaacattact ttccgtgaga ccgtcggttg
    55021 agagattttc gccaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgca
    55081 cccagtagag atagtgctca cgaggataat gacacagcgc tctgtctggc ccgccgtgaa
    55141 cctttttctc cgcctgctcg tcaccttcca gccccagctc cgtcagcatc aactcaccat
    55201 caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg
    55261 tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac
    55321 catatttatt ttatgtcagc cgtgctgttt tcgcggtaag tatccaggca ggaactcaat
    55381 agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc
    55441 accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag
    55501 gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcactaccag cgaagttgct
    55561 gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc
    55621 gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat
    55681 ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc
    55741 cccagcaaac cagtctgggc aatcacagtc aaatcaatgg tattgcccag cgcgaaggca
    55801 aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt ctcgtaattc agggtcaaga
    55861 ttccccaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca
    55921 aacgaggcaa tcccggcagt gcccagaata atcatcgtca tgagcggccc ggactccaac
    55981 gacatcaaca caaatgcccc agcttcttct ttcgtaccgt actgctgcat gatggaagcg
    56041 taaagtccgc cgttggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca
    56101 aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc
    56161 cacgcgacgg caattttagt taccaccagc gtaccggatt tacgcagtac cgttcccgtc
    56221 gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta
    56281 ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg
    56341 tggcacagtg cgccaaggaa tagcgggacg agcatcatcc cccccgggat tttctcaatc
    56401 gagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg atgcgtaatt
    56461 cctcaatgga gcatcagtgt tgatggaaac gaagatagac attcatttaa tcaataaaag
    56521 tgatcaaaac cacatcaatt gaaacgctgt tttattttta taggatcatt gtttttattt
    56581 tggaattgat cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta agctgatatg
    56641 cggcctatac gcctcattgc agcaggcggc aaatgattat tttttcgccg caaaacgtgc
    56701 cgctgcttcg tcccagttca ccacgttcca gaactctttg atgtagtccg gacggcggtt
    56761 ctggaatttc aggtagtaag catgttccca cacatccagg cccagaatcg ggaagccgga
    56821 agcgccagaa atagcttcac ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc
    56881 cagtttatcg cctttcagca ccagccatgc ccagccggaa ccaaagcggg aagctgccgc
    56941 tttttcaaat tctgctttga agttatcaac ggagccgaag tcacgttcga tagccgcttt
    57001 caggtcaccc tgcagggtgg tgcctttttt cagacctttc cagaacaggc tgtggttagc
    57061 gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct gctggcagct ggtccagttt
    57121 ggtgatcagc tcttcaaccg gcaggttggc aaattctggc aggctttcca gcgccgcgtt
    57181 ggcgttgttt acgtaggtct gatggtgttt ggtgtggtgg atttccatgg tctgcttatc
    57241 gaagtgcggt tccagggcat cgtaagcata cggcagggat ggcagggtat agctcatatt
    57301 catctccagt attgtcgggc ggccgattgt taatgccgcg taagcagttg gttcattata
    57361 gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt
    57421 tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa
    57481 atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc
    57541 gtcggagatg tgaagcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga
    57601 tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca
    57661 aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga
    57721 ttattctgcc gtgggccatc agcgccctgt tactaccgga tttctgggcg tattacagct
    57781 cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg aggatcggta
    57841 atatcaacta tggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca
    57901 ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg
    57961 tgctgattaa caccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg
    58021 gcgtagggat tgtaactcga gccgggcagt tgaaagagcg caagatgggc attaaagccg
    58081 aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt ttctctgccg
    58141 ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcacttggcg
    58201 tcgatccact gtatgtcgct ctgccaagct atgttgtcat catgggcggc ggcgcgatca
    58261 ttaacctcgg tttctgtttt attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg
    58321 acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt actctcggca ctgggcgggt
    58381 tgatgtggta tctgcaattc tttttctatg cctagggcca cgcccgcatt ccggcgcagt
    58441 atgactacat cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg
    58501 ggctggtgct gaaagagtgg aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg
    58561 gttgtgtggt gattattgtc gccgccaaca tcgtcggcat cggcatggcg aattaatctt
    58621 tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca
    58681 ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc
    58741 ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga tattgattga
    58801 tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct
    58861 catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc cgggtaatca
    58921 gcttatccag caacgtttcg ctggatgttg gtggcaacga atcactggtg taacgatggc
    58981 gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacggca
    59041 catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca
    59101 cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct
    59161 gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat
    59221 cgttaacgga agcgtaggag tgtttatcgt cagcatgaat ataaaagaga tcgccacggg
    59281 taatgcgata aggacgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca
    59341 gctcacaaaa atcatgtgta tgttcggcaa agacatcttg cggataacgg tcagccacag
    59401 cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca
    59461 ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt
    59521 tgagaagatt cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac
    59581 cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga
    59641 taggcgatgt cagtgacgct ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt
    59701 cgcaggcggt tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt
    59761 agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca
    59821 aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc
    59881 tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg
    59941 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    60001 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct
    60061 tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc
    60121 acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg atcgcgtacg
    60181 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    60241 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc
    60301 gggagccgga gttctatcgc cacggacgcg ttacaagacg gaaaaaaatc cacactatgt
    60361 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    60421 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    60481 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaattcagc aaattgtgaa
    60541 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    60601 ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    60661 gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc
    60721 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg
    60781 gctgcatagc caaaacggtt atgtcacctg ggatgtggat agcctggaaa gtgccattcg
    60841 ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac
    60901 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc
    60961 ttatcgcgat agccgcacca atggcctgat gccgcaggca caacagcaac tcggcaaacg
    61021 cgatatttat cagcgtagcg gcatccagtt tctacccttc aatacgcttt atcagttgcg
    61081 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    61141 ggattacttc agttatcgcc tgaccggcaa gatgaactgg gaatatacca atgccaccac
    61201 cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    61261 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat
    61321 ttgcccacag ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc
    61381 ggttatcgcc tcgccgttaa acggttcacg cgccgcttat ctctcttctg gcacctggtc
    61441 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat
    61501 caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    61561 gctgcttcag cgagtgcttc aggaacggca aatcaacgat ctcccggcgc ttatcgccgc
    61621 gacacaggca cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa
    61681 tcctgacgag atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    61741 gggaagtgat gctgaactgg cgcgctgtat tttcgacagt ctggcgctgc tgtatgccga
    61801 tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc tcgcaactgc atattgtcgg
    61861 cggcggctgc cagaacacgc tgctcaacca gctatgcgcc gatgcctgcg gtattcgggt
    61921 gatcgccggg cctgttgaag cctcaacgct cggcaatatc ggcatccagt taatgacgct
    61981 ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg cgaatctgac
    62041 cacctttacc cctaatcctg acagtgaaat tgcccactat gtggcgcaga ttcactctac
    62101 acgacagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg
    62161 aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca acttgatcgt
    62221 ttacccgttt caatgcactg ctggcagggc gatgatgttt ccggttttga aaacccggaa
    62281 ggttcgctga ccggggggat tcaggccaca ggcaattatc cgggcaaagc gcgtaatgcc
    62341 agtgagctac gtgccgatct ggaacaggct atgcggctga ttccggggcc gaaacggctt
    62401 aatttacatg ccatctatct ggaatcagat acgccagtct cgcgcgacca gatcaaacca
    62461 gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac
    62521 ccctcctgct tttcgcatcc gctaagcgcc gatggcttta cgctctcgca tcccgacgac
    62581 agcattcgcc agttctggat tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt
    62641 ggcgagcaac tcggcacacc atcggtgatg aacatctgga tcccggatgg tatgaaagat
    62701 atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc
    62761 agcgagaagc taaaccctgc gcaccatatc gacgccgttg agagcaaatt gtttggcatt
    62821 ggcgcagaga gctacacggt tggctccaat gagttttaca tggggtatgc caccagccgc
    62881 cagactgcgc tgtgcctgga cgccgggcac ttccacccga ctgaagtgat ttccgacaag
    62941 atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagccg tccggttcgc
    63001 tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcaattgc cagtgagatt
    63061 gtgcgtcacg atctgtttga ccgggtgcat atcggccttg acttcttcga tgcctctatc
    63121 aaccgcattg ccgcgtgggt cattggtaca cgcaatatga aaaaagccct gctgcgtgcg
    63181 ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt
    63241 ctggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtctggga aatgtattgc
    63301 caacgtcacg atacgccagc aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa
    63361 gcgattttga gccagcgtgg gtaaacactg ccggatgcgg cacgagcgcc ttatccggcc
    63421 tacgggtcga atgtccatgc gggctgtcac cctcaccccg gccctctccc tgaaagggcg
    63481 agggagaaaa acatagcctc gtgcggcaac ggagctgaac accgctccgg acagtcccct
    63541 cgcccctacg gggagagggt tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc
    63601 aacctaattt acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg
    63661 aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgagcgca acggcggcaa
    63721 cctgacgctg cgccaggatg acgccgatat cgcgctttat cacgacaact tccacccaca
    63781 accgcgctat attccgctca gccaacccat gcctttactg gcaaatacgc cgtttattgt
    63841 caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta acttaggcgt
    63901 cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa ccaacgaagc
    63961 cgttccaact tctgaacttc cggctcactt tctctcccac tgcgagcgca ttaaagccac
    64021 caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg ccctcaccta
    64081 tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca gcaccgaatg
    64141 tctggtagta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg gcacggacga
    64201 aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca
    64261 cggtgtgttc ggttccggac cgacgctgga tgaagccttt ggtttaatcg acaccgcaga
    64321 aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag
    64381 ccgtgaagag ttgatagcgc tcggcaagcg cttcgccgtt acaccactag ccagtgcgct
    64441 ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtaa accccgacgc
    64501 ccacgaagag tatcagcgtc ggcataatcc catctggccg gaactggaag cagtgttgaa
    64561 atctcacggt gtgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc
    64621 catggtagag attgaatctg aagaacggtg gaatgcggta gccagtactg atgtttgcca
    64681 acgttggtgg aaatatatga ccgatgttat gcccgctaac ccagataaca gcccggtgag
    64741 tagcgagctg caagaagtgt tttacctgcc gtaattgccc aatgtgccgg agatgcctcc
    64801 ggcacaatat tttaaatttc gatttgaaaa attaaacata ttacatctcc cggtaagtta
    64861 tatttccctg atacattgtg agtaaatcac aaaaataatg aataacccat taatgattca
    64921 tgtggtttat ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt
    64981 ctcaaattgt cattgtttac cgacatatcg cctctcatc
//
LOCUS       DADKXM010000007        64310 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.007, whole genome shotgun sequence.
ACCESSION   DADKXM010000007 DADKXM010000000
VERSION     DADKXM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.007"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..380
                     /locus_tag="JQR05_000429"
     CDS             <1..380
                     /locus_tag="JQR05_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322675.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVN
                     AGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
     gene            516..1436
                     /gene="speB"
                     /locus_tag="JQR05_000430"
     CDS             516..1436
                     /gene="speB"
                     /locus_tag="JQR05_000430"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY5322676.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1642..2400)
                     /locus_tag="JQR05_000431"
     CDS             complement(1642..2400)
                     /locus_tag="JQR05_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5322677.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVAPGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            2678..4669
                     /gene="tkt"
                     /locus_tag="JQR05_000432"
     CDS             2678..4669
                     /gene="tkt"
                     /locus_tag="JQR05_000432"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY5322678.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            complement(4715..5623)
                     /locus_tag="JQR05_000433"
     CDS             complement(4715..5623)
                     /locus_tag="JQR05_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY5322679.1"
                     /translation="MKAPVSLTSFHPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            6001..6444
                     /gene="cmtB"
                     /locus_tag="JQR05_000434"
     CDS             6001..6444
                     /gene="cmtB"
                     /locus_tag="JQR05_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY5322680.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            6472..7860
                     /locus_tag="JQR05_000435"
     CDS             6472..7860
                     /locus_tag="JQR05_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY5322681.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITCLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLPVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7875..9152
                     /locus_tag="JQR05_000436"
     CDS             7875..9152
                     /locus_tag="JQR05_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5322682.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            9149..10005
                     /gene="yggF"
                     /locus_tag="JQR05_000437"
                     /pseudo
     CDS             9149..10005
                     /gene="yggF"
                     /locus_tag="JQR05_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            10027..10536
                     /gene="fumE"
                     /locus_tag="JQR05_000438"
     CDS             10027..10536
                     /gene="fumE"
                     /locus_tag="JQR05_000438"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY5322683.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10533..11246
                     /locus_tag="JQR05_000439"
     CDS             10533..11246
                     /locus_tag="JQR05_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY5322684.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(11218..11895)
                     /locus_tag="JQR05_000440"
     CDS             complement(11218..11895)
                     /locus_tag="JQR05_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY5322685.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKFRQRGTSVVAISHDAAFTRRHFSRMVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(11889..12566)
                     /locus_tag="JQR05_000441"
     CDS             complement(11889..12566)
                     /locus_tag="JQR05_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY5322686.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12554..12937)
                     /locus_tag="JQR05_000442"
     CDS             complement(12554..12937)
                     /locus_tag="JQR05_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY5322687.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(12953..13261)
                     /locus_tag="JQR05_000443"
     CDS             complement(12953..13261)
                     /locus_tag="JQR05_000443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322688.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(13262..13840)
                     /locus_tag="JQR05_000444"
     CDS             complement(13262..13840)
                     /locus_tag="JQR05_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY5322689.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13863..14294)
                     /locus_tag="JQR05_000445"
     CDS             complement(13863..14294)
                     /locus_tag="JQR05_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5322690.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14666..15685
                     /gene="epd"
                     /locus_tag="JQR05_000446"
     CDS             14666..15685
                     /gene="epd"
                     /locus_tag="JQR05_000446"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY5322691.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15735..16898
                     /gene="pgk"
                     /locus_tag="JQR05_000447"
     CDS             15735..16898
                     /gene="pgk"
                     /locus_tag="JQR05_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY5322692.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            17113..18192
                     /gene="fbaA"
                     /locus_tag="JQR05_000448"
     CDS             17113..18192
                     /gene="fbaA"
                     /locus_tag="JQR05_000448"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY5322693.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            18383..19243
                     /locus_tag="JQR05_000449"
     CDS             18383..19243
                     /locus_tag="JQR05_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY5322694.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19382..20017
                     /gene="argO"
                     /locus_tag="JQR05_000450"
     CDS             19382..20017
                     /gene="argO"
                     /locus_tag="JQR05_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY5322695.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            20110..20850
                     /locus_tag="JQR05_000451"
     CDS             20110..20850
                     /locus_tag="JQR05_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY5322696.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            21017..21913
                     /locus_tag="JQR05_000452"
     CDS             21017..21913
                     /locus_tag="JQR05_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5322697.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(21910..23388)
                     /gene="scpC"
                     /locus_tag="JQR05_000453"
     CDS             complement(21910..23388)
                     /gene="scpC"
                     /locus_tag="JQR05_000453"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY5322698.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYNNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23412..24197)
                     /gene="scpB"
                     /locus_tag="JQR05_000454"
     CDS             complement(23412..24197)
                     /gene="scpB"
                     /locus_tag="JQR05_000454"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY5322699.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDNLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(24208..25203)
                     /gene="meaB"
                     /locus_tag="JQR05_000455"
     CDS             complement(24208..25203)
                     /gene="meaB"
                     /locus_tag="JQR05_000455"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY5322700.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMMLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDSDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSME
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(25196..27335)
                     /gene="scpA"
                     /locus_tag="JQR05_000456"
                     /pseudo
     CDS             complement(25196..27335)
                     /gene="scpA"
                     /locus_tag="JQR05_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(27539..28432)
                     /locus_tag="JQR05_000457"
     CDS             complement(27539..28432)
                     /locus_tag="JQR05_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY5322701.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28815..29474
                     /gene="rpiA"
                     /locus_tag="JQR05_000458"
     CDS             28815..29474
                     /gene="rpiA"
                     /locus_tag="JQR05_000458"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY5322702.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29730..30962
                     /locus_tag="JQR05_000459"
     CDS             29730..30962
                     /locus_tag="JQR05_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY5322703.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            31191..31250
                     /gene="ibsC"
                     /locus_tag="JQR05_000460"
     CDS             31191..31250
                     /gene="ibsC"
                     /locus_tag="JQR05_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY5322704.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31583..31642
                     /locus_tag="JQR05_000461"
     CDS             31583..31642
                     /locus_tag="JQR05_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY5322705.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(31743..32345)
                     /locus_tag="JQR05_000462"
     CDS             complement(31743..32345)
                     /locus_tag="JQR05_000462"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY5322706.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32366..32549)
                     /gene="ssrS"
                     /locus_tag="JQR05_000463"
     ncRNA           complement(32366..32549)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JQR05_000463"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32591..32920)
                     /gene="zapA"
                     /locus_tag="JQR05_000464"
     CDS             complement(32591..32920)
                     /gene="zapA"
                     /locus_tag="JQR05_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY5322707.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            33088..33666
                     /gene="ygfB"
                     /locus_tag="JQR05_000465"
     CDS             33088..33666
                     /gene="ygfB"
                     /locus_tag="JQR05_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY5322708.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33692..35017
                     /gene="pepP"
                     /locus_tag="JQR05_000466"
     CDS             33692..35017
                     /gene="pepP"
                     /locus_tag="JQR05_000466"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY5322709.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            35014..36192
                     /gene="ubiH"
                     /locus_tag="JQR05_000467"
                     /gene_synonym="visB"
     CDS             35014..36192
                     /gene="ubiH"
                     /locus_tag="JQR05_000467"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY5322710.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLVAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            36215..37417
                     /gene="ubiI"
                     /locus_tag="JQR05_000468"
                     /gene_synonym="visC"
     CDS             36215..37417
                     /gene="ubiI"
                     /locus_tag="JQR05_000468"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY5322711.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37491..37574)
                     /locus_tag="JQR05_000469"
     CDS             complement(37491..37574)
                     /locus_tag="JQR05_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322712.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            37865..38959
                     /gene="gcvT"
                     /locus_tag="JQR05_000470"
     CDS             37865..38959
                     /gene="gcvT"
                     /locus_tag="JQR05_000470"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY5322713.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38983..39372
                     /gene="gcvH"
                     /locus_tag="JQR05_000471"
     CDS             38983..39372
                     /gene="gcvH"
                     /locus_tag="JQR05_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY5322714.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39491..42364
                     /gene="gcvP"
                     /locus_tag="JQR05_000472"
     CDS             39491..42364
                     /gene="gcvP"
                     /locus_tag="JQR05_000472"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY5322715.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            42631..43374
                     /locus_tag="JQR05_000473"
     CDS             42631..43374
                     /locus_tag="JQR05_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5322716.1"
                     /translation="MAIALVTGGSCGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(43431..44864)
                     /locus_tag="JQR05_000474"
     CDS             complement(43431..44864)
                     /locus_tag="JQR05_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY5322717.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMCFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            44909..45220
                     /locus_tag="JQR05_000475"
     CDS             44909..45220
                     /locus_tag="JQR05_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY5322718.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            45384..46043
                     /locus_tag="JQR05_000476"
     CDS             45384..46043
                     /locus_tag="JQR05_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY5322719.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(46239..47219)
                     /gene="ygfZ"
                     /locus_tag="JQR05_000477"
     CDS             complement(46239..47219)
                     /gene="ygfZ"
                     /locus_tag="JQR05_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY5322720.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTALAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            47462..47728
                     /gene="sdhE"
                     /locus_tag="JQR05_000478"
     CDS             47462..47728
                     /gene="sdhE"
                     /locus_tag="JQR05_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY5322721.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            47709..48116
                     /locus_tag="JQR05_000479"
     CDS             47709..48116
                     /locus_tag="JQR05_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HAY5322722.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(48156..48677)
                     /gene="fldB"
                     /locus_tag="JQR05_000480"
     CDS             complement(48156..48677)
                     /gene="fldB"
                     /locus_tag="JQR05_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY5322723.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            48789..49685
                     /gene="xerD"
                     /locus_tag="JQR05_000481"
     CDS             48789..49685
                     /gene="xerD"
                     /locus_tag="JQR05_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY5322724.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            49710..50420
                     /gene="dsbC"
                     /locus_tag="JQR05_000482"
     CDS             49710..50420
                     /gene="dsbC"
                     /locus_tag="JQR05_000482"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY5322725.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            50426..52159
                     /gene="recJ"
                     /locus_tag="JQR05_000483"
     CDS             50426..52159
                     /gene="recJ"
                     /locus_tag="JQR05_000483"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY5322726.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            52250..53348
                     /gene="prfB"
                     /locus_tag="JQR05_000484"
     CDS             join(52250..52324,52326..53348)
                     /gene="prfB"
                     /locus_tag="JQR05_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY5322727.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            53358..54875
                     /gene="lysS"
                     /locus_tag="JQR05_000485"
     CDS             53358..54875
                     /gene="lysS"
                     /locus_tag="JQR05_000485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY5322728.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFITLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(54918..55466)
                     /gene="idi"
                     /locus_tag="JQR05_000486"
     CDS             complement(54918..55466)
                     /gene="idi"
                     /locus_tag="JQR05_000486"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY5322729.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            55521..55592
                     /locus_tag="JQR05_000487"
     CDS             55521..55592
                     /locus_tag="JQR05_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322730.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(55589..55714)
                     /locus_tag="JQR05_000488"
     CDS             complement(55589..55714)
                     /locus_tag="JQR05_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322731.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(55716..57164)
                     /locus_tag="JQR05_000489"
     CDS             complement(55716..57164)
                     /locus_tag="JQR05_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY5322732.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            57600..59519
                     /gene="ygfT"
                     /locus_tag="JQR05_000490"
     CDS             57600..59519
                     /gene="ygfT"
                     /locus_tag="JQR05_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAY5322733.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQRIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFERHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEQGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGAAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            59519..59962
                     /locus_tag="JQR05_000491"
     CDS             59519..59962
                     /locus_tag="JQR05_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5322734.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDIISAPVMCHQCENAPCVGACPVQALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELR"
     gene            complement(60043..61410)
                     /locus_tag="JQR05_000492"
     CDS             complement(60043..61410)
                     /locus_tag="JQR05_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5322735.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(61446..62762)
                     /gene="guaD"
                     /locus_tag="JQR05_000493"
     CDS             complement(61446..62762)
                     /gene="guaD"
                     /locus_tag="JQR05_000493"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HAY5322736.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPSKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLGYERN"
     gene            complement(62780..64180)
                     /gene="xanQ"
                     /locus_tag="JQR05_000494"
                     /pseudo
     CDS             complement(62780..64180)
                     /gene="xanQ"
                     /locus_tag="JQR05_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
BASE COUNT        15647 a        16097 c        17155 g        15411 t
ORIGIN      
        1 tcggaatgaa ttatcaatta tatgtccgta attacagtcg taacaccaac tacgagatcg
       61 ttgctacggt tgatggcctg gatgtgctga acggcaagca aggttcgctg aataacaacg
      121 gctatattgt gaatgccgga gattcgttag cgattaaagg attccgcaaa gataaacata
      181 ctgaagcggc ttttcagttc gccaacgttg ctgattccta cgcagccaat tcggcgcaag
      241 gcgatgtgcg taataccggg gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc
      301 aaaacgcgct gccgccgtgc tctggacaag cctttccggc agataacaat ggctatgcgc
      361 cgccaccgtg tcggaaatag caacctcgct tgatgcctcg cccatttaag gcgataatta
      421 gcacaaactt gtgtttcgca aatccaaccc ttcctcgtcg ggcctaacga cgcggaaggg
      481 tttttttata tcgactttgt aataggagtc catccatgag caccttaggt catcaatacg
      541 ataactcact ggtttccaat gcctttggtt ttttacgcct gccgatgaac ttccagccgt
      601 atgacagcga tgcagactgg gtgattactg gcgtgccgtt cgatatggcc acttctggtc
      661 gtgcgggtgg tcgccacggt ccggcagcga tccgtcaggt ttcgacgaat ctggcctggg
      721 aacacaaccg cttcccgtgg aatttcgaca tgcgtgagcg tctgaacgtc gtggactgcg
      781 gcgatctggt atatgccttt ggcgatgccc gtgagatgag cgaaaagctg caggcgcacg
      841 ccgagaagct gctggctgcc ggtaagcgta tgctctcctt cggtggtgac cactttgtta
      901 cgctgccgct gctgcgtgct catgcgaagc atttcggcaa aatggcgctg gtacactttg
      961 acgcccacac cgatacctat gcgaacggtt gtgaatttga ccacggcact atgttctata
     1021 ccgcgccgaa agaaggtctg atcgacccga atcattccgt gcagattggt attcgtaccg
     1081 agtttgataa agacaacggc tttaccgtgc tggacgcctg ccaggtgaac gatcgcagcg
     1141 tggatgacgt tatcgcccag gtgaaacaga ttgtgggtga tatgccggtt tacctgactt
     1201 ttgatatcga ctgcctggat cctgctttcg cgccgggaac cggtacgcca gtgattggcg
     1261 gcctgacctc cgatcgcgcc attaaactgg tacgcggcct gaaagatctc aacatcgttg
     1321 ggatggacgt agtggaagtg gctccggcat acgatcagtc ggaaatcacc gctctggctg
     1381 cggcgacgct ggcgctggag atgctgtata ttcaggcggc gaaaaagggc gagtaagcac
     1441 cagatgcgat gcgcacgggt aaaacgtgcc attaatgtcg gatgcggcgt gaacgcctta
     1501 tccgacctac gttcggcacc cgtaggccgg ataagatgcg ccagcatcgc atccggcaat
     1561 gcgcacaagg taacaaatgt gccattcatg tcagatgcgg cgtgaacgcc ttatctgacc
     1621 tacgttcgac accaccaggc tttacttaat cccatccgcg ctgatgcgat cgcgaatatg
     1681 ctgggcacgt tcggcggatg caggatggtc gtcaaacatt gagctttgac ggccttcttc
     1741 cagcttcgcc agtttttcaa agctggtggc aagacctgcc gggctgatgc cgcgttggcg
     1801 cagaagatcg taagaataat catcggcttc tgcttcctgg cgctgggaga attgcgaatt
     1861 gactaatttc tcgcccagat caccaagttg tgattgagat aaactgccga caatcccgcc
     1921 cgcagaggcc gcagctactc gcacggcatt tgtaccaggc gccacctgca tccctttctt
     1981 cacatggcct aacgccacgt gccccatctc gtgaccgatc accgcttcga cttcgttatc
     2041 tgtcatcata tccatcagcc cgctatagac gcggatacag ccgttagcca ttgcaaaggc
     2101 gttcacatcc ttcgccatat acactttgta attcaccggc tgaccgttga tattgttgcc
     2161 tagcgcattg gcaatagttg tcagacgttt agcgtattcg ctattggctg gcgcaatcgt
     2221 cgccttgctg tccatctcct gacatgcctg atcgctcagg gttttcacct gcgcatcact
     2281 caaactgtaa gcctgaaaag cttccgctcc tgatgagagc agtccgttgg agtccatatt
     2341 ctggcaaccg gtcagtaccg ttgccacgct cattgctacc aataaggcgc gaattttcat
     2401 gttttctctt ccgcacaagg tcaggattat tctaaaaatg cgccgtttgc aggtgaatcg
     2461 acgctcagtc tcagtataag gaaaagcgca gcggacgggc gagcagattg cgcaacatgc
     2521 gagcatgatc cagagatttc tgaagcggca aaaggatgtt ccatgtacat gacgcgcggc
     2581 ttgcggtaaa ttgttggcaa attttccggc gtagcccaaa acgcgctgtc gtcaagtcgt
     2641 taagggcgtg cccttcatca tccgatctgg agtcaaaatg tcctcacgta aagagcttgc
     2701 caatgctatt cgtgcgctga gcatggacgc agtacagaaa gccaaatccg gtcacccggg
     2761 tgcccctatg ggtatggctg acattgccga agtcctgtgg cgtgatttcc tgaaacacaa
     2821 cccgcagaat ccgtcctggg ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc
     2881 catgctgatc tacagcctgc tgcacctcac cggttacgat ctgccgatgg aagaactgaa
     2941 aaacttccgt cagctgcact ctaaaactcc gggccacccg gaagtaggtt ataccgctgg
     3001 tgtggaaacc accaccggtc cgctgggtca gggtattgcc aacgcagtcg gtatggcgat
     3061 tgcagaaaaa acgctggcgg cgcagtttaa ccgtccgggc cacgacattg tcgaccacta
     3121 cacctacgcc ttcatgggcg acggctgcat gatggaaggc atctcccacg aagtttgctc
     3181 tctggcgggt acgctgaagc tgggtaaact gattgcgttc tacgatgaca acggtatttc
     3241 tatcgatggt cacgttgaag gctggttcac cgacgacaca gcaatgcgtt tcgaagctta
     3301 cggctggcac gttattcgcg acatcgacgg tcatgacgcg gcatccatca aacgcgcagt
     3361 tgaagaagcg cgcgcagtga ctgacaaacc gtccctgctg atgtgcaaaa ccatcatcgg
     3421 tttcggttcc ccgaacaaag ccggtactca cgactcccac ggtgcgccgc tgggcgacgc
     3481 tgaaattgcc ctgacccgcg aacagctggg ctggaaatat gcaccgttcg aaatcccgtc
     3541 tgaaatctat gctcagtggg atgcgaaaga agcaggccag gcgaaagaat ctgcatggaa
     3601 tgagaagttt gcggcttacg cgaaagctta tccgcaggaa gcggctgaat ttacccgccg
     3661 tatgaaaggc gaaatgccgt ctgacttcga cgccaaagcg aaagagttta tcgctaaact
     3721 gcaggctaat ccggcgaaaa tcgccagccg taaagcgtcg cagaatgcta tcgaagcgtt
     3781 cggcccgctg ttgcctgaat tcctcggcgg ctctgctgac ctggcaccgt ctaacctgac
     3841 cctgtggtct ggttctaaag caatcaacga agatgctgca ggtaactaca tccactacgg
     3901 tgttcgcgag ttcggtatga ccgcgattgc taacggtatc tccctgcacg gtggcttcct
     3961 gccgtacacc tccaccttcc tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc
     4021 tgcgctgatg aaacagcgcc aggtgatggt ttacacccac gactccatcg gtctgggcga
     4081 agatggcccg actcaccagc cggtagagca ggtcgcttct ctgcgcgtga ccccgaacat
     4141 gtctacatgg cgtccgtgtg accaggttga atccgcggtc gcgtggaaat acggcgttga
     4201 gcgtcaggac ggcccgactg cgcttatcct ctcccgtcag aacctggcgc agcaggaacg
     4261 tactgaagag cagctggcaa acatcgcgcg cggtggttat gtgctgaaag actgtgctgg
     4321 tcagccggaa ctgatcttca tcgccaccgg ttctgaagtt gaactggcgg ttgctgccta
     4381 cgaaaaactg actgccgaag gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc
     4441 atttgacaag caggatgcgg cttaccgtga atccgtactg ccgaaagcgg ttactgcacg
     4501 cgttgcggta gaagcgggta ttgctgacta ctggtacaag tatgttggcc tgaacggcgc
     4561 tatcgtcggc atgaccacct tcggtgaatc tgctccggca gagctgctgt ttgaagagtt
     4621 cggcttcact gttgataacg ttgttgcgaa agcaaaagcg ctgctgtaat cagtaagacg
     4681 ttttgtgaga agggccgcga aagcggccct ttttttactt agtagagata aacggaactg
     4741 tgctgccggg gatcatggta tcgggcagtt taccgtccca acgttcggca gtggtgaggg
     4801 ctacaagtcc gggattatcc cgcagagctt caccgcgcag gcggattgct tctgcttcag
     4861 ccgcactttt taaacgaata gtttcagcct ctgccgcgcc tcttacgcga atagtttccg
     4921 cttctgcttt ggctgccgcc agtttactgt ctgcttctgc ctgggcctga gttaccgcta
     4981 tttgcgcctg gattttctct gtttccagat tttgcttacg cgtggcaatg gcgacttctg
     5041 ctttcattcg gtcttcaatc gacttttcat aagcgtcaga aaaatcaata ttttcaatct
     5101 ggaccccgtc aattaccact ggcccaacaa cggctttgcg catggcattt tgcagatcct
     5161 gaaccaattt tgtacgatcc tgtacggcgc tgatggcggt atattggccg aaaatgttct
     5221 ccagctgagt tggcaactga cggacaatta gacgatcttt taatgcttca atagtgttgt
     5281 aggtggtata aaccgcgcct gcttctgaag gtttaatgtg gaagctgact gatactgtca
     5341 tttgtgctgg ttgctggtcg cggctgtagg cctgtaatcc ctgatagacc acagcctgat
     5401 tacgcgtgga aattttctcc acgctttcca taaaagggat cttaaaaccc agccccggct
     5461 cggcaacttt tacgattttt ccgtagcgca gcaaaatgcc gcgttcacct tcattaacgg
     5521 tgtaatacga gagaaatggc agcacaacca cggcaagcac gccaatggcg atagcaagag
     5581 atttttgtgg gtgaaatgag gttaatgaaa caggtgcttt catgacaatc ttccttgagt
     5641 aaagtatggg aatatgtcac atgagatgag gagaaaacag ccgacaggtg agaattcaga
     5701 atctataaag gggtatagcg aaaaaacatt gcattagaaa agtatgtttt cgaacattca
     5761 ataaataaat agagatatgc atcacaaata caataacaaa caaaatgttg attttgtgac
     5821 gaatataaca aatacaaagt tgactttaaa aatatcaatt tcatattgtt gttatttaag
     5881 gttttattat tttggtttat ggggtttttc ttctcgcgta cccaattgct ttcattaaac
     5941 cgcacgagta ttttattttt accgccggac ggtaccctaa aaataaaata aggataattt
     6001 atgcggctta gtgattattt tccagaatca tcaatctcag ttatacattc agcaaaagat
     6061 tggcaggaag ctatcgattt ctcgatggta tcattgctgg ataaaaacta tatcagcgag
     6121 aattacattc aggcaattaa agattccacc attaacaatg gcccttatta tattctcgca
     6181 ccaggcgtgg caatgcctca tgcgcgaccg gaatgtgggg cgcttaaaac cgggatgtct
     6241 ttgacattac ttgaacaagg tgtttatttc ccggggaatg acgaaccaat taaattactc
     6301 atcggactct ctgctgccga tgccgattcg cacattggcg ctattcaggc gttaagtgaa
     6361 ttactgtgcg aagaagaaat actcgaacaa ctcttaacag catcatcaga aaaacaatta
     6421 gcggacatta tcagccgcgg ataattactc tctcctctac aggataatat tatggaaaac
     6481 aagtctgctc gtgcaaaggt ccaggctttt gggggatttt tgacggcaat ggtcatcccc
     6541 aatataggtg cttttattgc ctggggcttc ataactgcgt tatttattcc caccggttgg
     6601 ctgcctaacg aacatttcgc caaaattgtc ggcccgatga ttacctgttt attgcccgtg
     6661 atgattggtt ctacaggtgg tcatctggtc ggcggtaaac gcggggcggt catgggcgga
     6721 ataggtacta ttggtgtgat cgttggcgca gagatcccga tgttccttgg ctcaatgatc
     6781 atgggaccgc tcggtgggtt ggtcataaaa tatgtcgata aggcactgga aaaacgcata
     6841 cctgccggtt ttgagatggt tatcaataac ttctcattag gtatcgcggg gatgctcctt
     6901 tgtctgctgg gttttgaagt gatcggcccg gcggtgttaa ttgccaatac tttcgtcaaa
     6961 gagtgtattg aggcgctggt acatgcgggt tatctgcctc tgctgccagt catcaatgaa
     7021 ccggcgaaag tgcttttcct taataatgcg atcgatcagg gcgtctatta tccgctggga
     7081 atgcaacagg cttcggttaa cggtaaatcc atcttcttta tggtggcctc taacccaggt
     7141 ccgggcctgg ggctgctgct ggcgtttacc ttgtttggta aagggatgag taaacgttct
     7201 gcgcccgggg cgatgattat tcacttcctc ggtgggatcc acgaactgta tttcccatat
     7261 gtgctgatga agccgctgac tattattgcc atgattgcgg gcggtatgtc tggtacctgg
     7321 atgtttaact tactggacgg tggtctggtg gctggcccaa gtccggggtc tatctttgcg
     7381 tacctggcac tgacgccgaa aggctcgttc ctggcgacaa ttgccggtgt tacggtaggt
     7441 accctggtgt cctttgctat tacttcgctg atactgaaga tggaaaaaac ggtggaaacg
     7501 gagagcgaag atgagtttgc tcagtcagcc aatgcggtta aggcgatgaa acaagagggt
     7561 gcattctcgt taagcagggt taagcgtatc gcctttgttt gcgatgcggg gatgggctcc
     7621 agtgcgatgg gcgcgaccac cttccgtaaa cgcctggaaa aagcggggct ggcaatagaa
     7681 gtaaaacatt acgcgataga aaacgtgcct gcggatgccg atatcgtcgt cactcacgcc
     7741 agtctggaag ggcgcgtgaa acgtgtgacg gataaaccgc tgatattgat taataactat
     7801 attggcgatc caaaactcga cactttattt aatcaattaa ccgccgaaca taaacactga
     7861 ttacagaggt aaaaatgaaa accaaagttg ctgctattta tggcaagcgg gatgtccgtc
     7921 tgcgcgaatt tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg
     7981 acagcgtctg tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac
     8041 ccgacgattt agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg
     8101 aagtgggtaa aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg
     8161 cgatggggtt accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg
     8221 ccacttatat gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg
     8281 gctcttattt tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc
     8341 atgccaatta tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg
     8401 gcaatattgc actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca
     8461 ttaacggtgg catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg
     8521 cgcaggtgca gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctggtgt
     8581 atgtgaatac caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg
     8641 tcgggttcga tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg
     8701 aattactggc ggaagatggc tgtctgaact tctttgccgg gccgacggat aaaaacttca
     8761 aagtgccgtt taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg
     8821 gcggttcaac ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc
     8881 cgtcgtttat ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc
     8941 tgccggatat ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg
     9001 ccattgccga ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc
     9061 tggttgagga aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat
     9121 ttggcgttga tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt
     9181 tacggaacag gcagcgttgg ctgcctggcc gcaaaccgga tgtggcgaca aaaataaaat
     9241 tgatggcctg gcggtcaccg cgatgcgcca gaagtcgata tcgcggttga tcccattgaa
     9301 ggcacgcgga tggtggcgat ggggcagagc aatgcgctgg cggtcatggc tttcgctcct
     9361 cgagatagcc tgttacatgc gcccgatatg tatatgaaaa agctggttgt taatcgactg
     9421 gctgccgggg cgattgatct gtctttgcct ctggcggaca acttgcgtaa cgtggcgcgg
     9481 gcgttaggta aaccgctgga caagctgcgc atggttacgc tggataaacc gcgtctgagt
     9541 gccgcgattg aagaagcaac acagctgggc gtgaaggttt ttgccctgcc ggatggcgat
     9601 gtcgccgcca gcgtgctgac ctgctggcag gataatcctt acgatgtgat gtacaccatc
     9661 ggcggtgcgc cagaaggcgt gatttctgcc tgtgccgtta aagcgttagg cggggatatg
     9721 caggccgagc tgattgactt ttgtcaggct aaaggggatt acacggaaaa tcggcagatt
     9781 gcagagcagg agcgcaagcg ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg
     9841 ctcgatgaac tggtgagggg gaacgatatc ctctttagcg ctacgggcgt gacgggcggc
     9901 gagttggtga acggtatcca acagatggcg aatggggtgc ggacgcagac attactgatc
     9961 ggcggcgcgg accaaacgtg taatataata gactccctgc attgatggtg atctatcgga
    10021 agaccaatgg cgacgctgac agaagatgat gtgcttgagc aactggatgc acaggacaat
    10081 ttattttcat ttatgaaaac tgcgcattct attttgctcc aggggatacg ccagtttctg
    10141 ccgtcgctgt ttgtcgataa cgatgaagag atcgtcgaat atgcagtgaa gccgttactc
    10201 gcccaaagcg gcccgcttga cgatattgat gttgcgctgc gtttgattta tgcgctgggg
    10261 aaaatggata aatggctgta tgccgatatc acgcactttt cccagtactg gcattacctg
    10321 aacgaacagg atgaaacgcc cggatttgcc gatgatataa cctgggattt tatcagcaat
    10381 gtcaacagta taactcgcaa tgcgacgctc tacgatgcgt taaaagcgat gaagttcgcc
    10441 gatttcgccg tctggtcaga ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg
    10501 gcagtaacga ccaccttaaa ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc
    10561 tgaacgtgca agcgcagtac catgatgaag agatagaacg cgtgcataaa ccgttgctgc
    10621 gtatgctggc ggccttacag acggtaaatc cgcagcggcg tacggtggtt tttctttgcg
    10681 ctccgccggg gacgggtaaa tctaccctaa ccaccttctg ggaatatctc gcgcaacaag
    10741 acccggagct gcccgctatt cagacgctcc cgatggatgg ttttcaccat tacaatagct
    10801 ggctggacgc gcatcaattg cgccccttca aaggcgcacc agagacattt gacgttgcga
    10861 aactggcgga aaatctgcgc caggtggtgg aaggggattg tacgtggccg cagtacgatc
    10921 gacaaaagca tgatcctgtt gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg
    10981 aaggaaactg gttgttgctg gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt
    11041 tctcgatctt tatcaacgcg ccagctactg cgttgcggga gcgtctggtg gggcgcaagc
    11101 tggcgggagg ggtatcgctg gcagatgcag aagcctttta tgaccgtacc gacgggccga
    11161 atgttcgccg ggtgctggaa gagagtctgc ctgcaaattt gaccttaatg atgaccgcta
    11221 cgggtgaata tcgtctggtg gattaacgtt cctgattaca ccatcttcca gtcgcaccat
    11281 acgagagaaa tgacgccggg taaatgcggc gtcatgactg attgctacca cgctcgtgcc
    11341 tcgctggcgg aacttctcca gccagctttc aaacacgctc aaccaatttt cgtcaaaatc
    11401 ccgactcggc tcgtccagca gtaatagtgg cggagagagt gcttccaggc aggcgacggc
    11461 gaccatccgt cgctgcgcac tatgcagatc caacgggtgc gagttggcag tgtctgctaa
    11521 ctgacaacat tgcagggcag catgggtacg ttgagtgatt tcatccgccg ggcatttttg
    11581 cagtttcaaa ccaaatgcga tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc
    11641 ttcctgaaac agtacgccaa cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc
    11701 ctgttgttgc agcataaccg taccggctgt gggcgtaaga agacctgcca ttactcgtaa
    11761 cagcgtcgat ttccctgcgc cattgtcacc ggtcagcgcc aaccattcac cttgtttgag
    11821 ttgcagagaa atgtcgcaaa gacaatccgt tgccgcgcca ggccagcgat aagatatctt
    11881 atttaacgtt aacatagtgg ctggagcgct ccgttttgca attgccacgc ttgctggcag
    11941 tagttcagga aaggtgtgcg atgacgttca aaaagaataa tcagcgaacc acgttcaaac
    12001 gcccagtgtt gtaagcgctg caacagcatt tcgctggcct gcggcgtcag gcggctaaag
    12061 gcttcatcaa gaattaagag ctttggctgc atcgcgatgg cgcaggcgat gaccacgcgc
    12121 tgtgtctctc caccggagag tgttgccggg tgacgatggc gcaacggctg gcactcggtc
    12181 agagtcaggg cggcatcaat ccgcgccata atctctgctt cagcaagaca cagattttct
    12241 ggcccaaagg caacttcttc ttccacgcta aaggtacagc cggagagctg caaatagggc
    12301 gattgctgga cgagctggat ggtggcggat tgttcattaa gcgggagatg cccaatgggc
    12361 gtaccgagga gcgtccccgt gcctgttatc tctcttggca gaaagtccgg ataccagccc
    12421 gccatcagct gtgccagcgt acttttgcca ctgccgttgt caccaaagat ggcgaccatc
    12481 cccggtttga catagtgaaa gtcataacag aacggaggat gcgttgagtg tgtcgggcaa
    12541 taacggaact gttctaacgt aaccatatcc acgctccgaa ttctgtcagc atcagcaaga
    12601 tcatggtgta acgcgctacg cgttgtaggg tgctatcggc tggtgcccac aacgttgttc
    12661 gtcggttgtg gatccgaaat gcccgcatat ccagcgccgc accacggaca gccagatcgt
    12721 taagcgcatt gtgtgttagc ggaataatta acgcgggcat agcacgtaaa cgctgatacc
    12781 agccctcatc cagcggcacg ccgcgggcac gctgcgcttc atgaataatc gctaattgcc
    12841 gttttaactg ctcaacgacc aataacggcc cggcaaacag ataagcgacg cctggcggca
    12901 ggcgagaagc aaacagagcg cgaataaaac attgcacggg gacgtactgc attcacaact
    12961 gtgaggttga aacaatcgcc agaatcagca accagagcgt aatagcatgc gaccaccgtt
    13021 ctggcgagcg tggcgtgccg cttagccatt ctggtagcca gccaccgtgt accagccaca
    13081 gcccggctcc cagcgagaac atcagccaga cgacatattt tgcccgccgc cgcgtggcct
    13141 ttagcgcaat aaggcagaaa aaggtcgccg cgctatagat cggcaaaatg gtctgcgcgg
    13201 gcaggataag cgtggtgcag gccgccaatg cccacagtgt taatgaggta aatggatgca
    13261 tttagcgcac ggcagccatt gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca
    13321 gtaaaagcca gacaatcacc gctgtaagaa ttttatcgac caggttcgcg cctatcaccg
    13381 taatcgccac cgactccacc aggttttgcc ccatcgagtg catccaggca acgaacaaat
    13441 cggctccgct accggtgaca ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg
    13501 ttaccgcaag cgtaataatc acgccgctga caacgacttt tggcagggtg cgaaaccaac
    13561 ccgctcgcgc cagccaaccg gcgaccaggc cgatgaccat cgcgacgggt gcaaatgcgg
    13621 cggcgatcgg gtcggtcagc aatccccaga gtaaattggt cagtaagccg gtcagcattc
    13681 cgatcaccgg accaagcagt accgcgctaa taagtgtgcc gatggagtcg agaaatatag
    13741 gtaatttgac catgctggcc agttgcccgc cgatcatatt gatggcgata gatataacga
    13801 tcaacaccag tgcctgactg gaaaagtggc tacgcgccat aagaaaacct ctttgaaagt
    13861 agttatttaa cgggggaaat atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg
    13921 ctaaacgcca gttcctcaag gctaccgatc accagttcaa gcgtggtccg cacggtacag
    13981 ccgggcatca tatgaccgcc aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga
    14041 tgctcaccac tctgttccag cgtgccgttt agtgagatca cttcgaatgt accgttcagg
    14101 agcgtggtgc cttcttgccc ggcatagcgc aaggcgacgt cagtcaggct accggtacag
    14161 cctgcaatcc aggcggcatg aagttgatgt tgctgtgcaa aagcgtgtag ctgggaaaaa
    14221 acttcctgcc cggggaggag gcgcagtgcg taaaagcgtg cggaggaaga atatgggtag
    14281 ggcaatgtca tcattggagt actccgaaac tcggacgtat tgcggataat atcgacgctg
    14341 catcagtcat attctgatat aagtcagggt ttgtgacggc acaacgaaaa gattttgcta
    14401 cgcttattcg tgattctgca aagtggaata aattccacag gggcggggct gaaaacgtga
    14461 cgtaagtcag ataattgctt tctttgactg gacaatcatt ccttttattc cacgtttcgc
    14521 ttatcctagc tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag
    14581 tttgcgacag gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc
    14641 tcagggaaaa ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg
    14701 catcgggcgt aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt
    14761 ggtggcaatc aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac
    14821 cagccatggc cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga
    14881 tgacgccatc cgcgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg
    14941 cgttgatgta gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca
    15001 tattgccgcc ggggccaaaa aagtgctctt ttcacatcct ggcagtaacg atctcgacgc
    15061 gaccgttgtt tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa
    15121 cgcttcctgt accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg
    15181 tattgagtcc ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga
    15241 tgcataccat cctgacctgc gtcgcacccg ggcagccagc cagtcgatca ttccggtcga
    15301 tactaaactg gccgctggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc
    15361 gattgcggta cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa
    15421 gaaacctgta aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt
    15481 tcatggtata gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc
    15541 gcacagtgcc attgtcgatg gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac
    15601 gttggtctgg tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc
    15661 tatggctact gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa
    15721 cgagaggatt caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg
    15781 tatttatccg tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc
    15841 gtatccgtgc ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg
    15901 taacttccca cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc
    15961 cggttgttaa ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc
    16021 tcgacggcgt tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca
    16081 aaggcgagaa gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt
    16141 tcgtaatgga cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta
    16201 aattcgctga cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta
    16261 aagcactgaa agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta
    16321 ccaaactgac cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg
    16381 gtatcgctaa cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag
    16441 ctgacctggt tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg
    16501 atgttcgcgt agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg
    16561 atgtgaaagc tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg
    16621 aaatcctgaa gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc
    16681 cgaacttccg caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct
    16741 ccatcgctgg cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa
    16801 tctcctacat ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc
    16861 ctgcagtagc gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc
    16921 tgcccttgtt tcagcgcgct tttagagcac gcattttttc atactctaaa taattcgagt
    16981 tgcaggaagg cgacaagaga gtgaatcccc aggagcttac ataagtaagt gactggggtg
    17041 agcgaacgca gacgcagcac atgcaacttg aagtattatg agtataaggc ccgacgatac
    17101 aggacaagag acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat cactggtgat
    17161 gacgtacaga aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac
    17221 tgcgtcggta ctgactccat caacgccgta ctggaaactg ctgctaaagt taaagcgccg
    17281 gttatcgttc agttctccaa cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct
    17341 gacgttccgc agggtgctgc tatcctgggc gcgatctctg gtgcgcatca cgttcaccag
    17401 atggctgaac attatggtgt tccggttatc ctgcacactg accactgcgc gaagaaactg
    17461 ctgccgtgga tcgacggtct gctggacgcg ggtgaaaaac acttcgcagc taccggtaag
    17521 ccgctgttct cttctcacat gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa
    17581 atctgctcta aatacctgga gcgtatgtcc aaaatcggca tgactctgga aatcgaactg
    17641 ggttgcaccg gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg
    17701 tacacccagc cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt
    17761 ttcaccatcg ctgcgtcctt cggtaacgta cacggtgttt acaagccggg taacgtggtt
    17821 ctgactccga ccatcctgcg tgattctcag gaatatgttt ctaagaaaca caacctgccg
    17881 cacaacagcc tgaacttcgt attccacggt ggttccggtt ctactgctca ggaaatcaaa
    17941 gactccgtaa gctacggcgt agtgaaaatg aatatcgata ccgataccca atgggcaacc
    18001 tgggaaggcg ttctgaacta ctacaaagcg aacgaagctt atctgcaggg tcagctgggt
    18061 aacccgaaag gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt
    18121 gccggtcaga cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc
    18181 gacgttctgt aagtcattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt
    18241 cgccaaaagc aaggtgattc agatgagaaa tctgtgatct atttggcaaa attatgcttt
    18301 attgtttacc cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga
    18361 gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc
    18421 gggaagctgg ctggtagcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc
    18481 ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa
    18541 tcgcctgatg atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt
    18601 ccgttacggt attatcgcct ttacgctaat cgctgccctg ggacgcgtgg gtgtacaaac
    18661 agcgtcagtc attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg
    18721 gtcactttct aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg
    18781 agaatatgtt gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac
    18841 caccatgcgt actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg
    18901 aaatattatt aacttctccc gcgagccagt tcgccgtaac gaatttatta ttggcgtggc
    18961 gtatgattcc gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga
    19021 tcgcattttg aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat
    19081 taatttcgtg gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt
    19141 gctggagcgt attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat
    19201 ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga
    19261 taatactctc tggcctctct cttattagtt attctgattg ccaattaata ttatcaattt
    19321 ccgctaataa caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa
    19381 cgtgttttct tattactttc aaggtcttgc tcttggggca gctatgattc tgccgctcgg
    19441 cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca ttatgattgc
    19501 cttactttgc gctatcagcg atttggtcct gatttgcgcg gggatttttg gtggtagcgc
    19561 gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg tagtcttctt
    19621 gctctggtat ggttttggcg cttttaaaac agcgatgagt agtaatattg agttagctag
    19681 tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc accatgttgg cagtgacctg
    19741 gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg gcgggcagct
    19801 tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt
    19861 ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca
    19921 gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc agctggcgag
    19981 agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa
    20041 acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga
    20101 gagactaacg tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg
    20161 gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagt
    20221 gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat
    20281 gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa
    20341 ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca gccagattat
    20401 gactatcagg atggtaaaag tatcctgaaa ggctaccgag ctgtgagaac ggtggaagtc
    20461 acgctccgtc agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt
    20521 aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg
    20581 cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat
    20641 cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca
    20701 atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac ttacgagcag
    20761 gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa
    20821 caacccgcta aaacacctgc agcacaataa tacttgtagc ccgacgtatt cgtcgggcta
    20881 atgcctttac ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc
    20941 acctatcttt gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag
    21001 gatgcgggga ggggggatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt
    21061 agcgcaaacc aataatattg cccgggcggc agaaaaaatc catatgacgg cttcgccatt
    21121 tggtaaaagc attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga
    21181 taataatatc agcctcaata aagccggtca ggaactgtat caaaaactgt tcccggttta
    21241 tcaacgactt tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat
    21301 tgtgattggc atagataata cttatccgac aattattttc gatcagttga ttagtctcgg
    21361 agataagtat gaaggcgtga ccgcccagcc agttgagttc agcgaaaacg gcgttatcga
    21421 taatcttttt gaccgtcagt tagattttat tatctcccca cagcatgtat ctgcacgagt
    21481 tcaggaactg gaaaacctga ctatcagtga attaccaccg ctgcgattgg gctttctcgt
    21541 ttctcgccgt tatgaagagc gtcaagagca agagctgctt caggagctgc cctggctaca
    21601 aatgcgcttt caaaaccggg ctaacttcga agcaatgatt gatgcgaata tgcgaccttg
    21661 tgggatcaat cccaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt
    21721 ggagcgcgga cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc
    21781 aacgctgaaa tattttgatg cgccgcacag gccgatgtat atgcaggaat atctttattc
    21841 aatcagaaat catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg
    21901 gacaaaccat taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac
    21961 gtggctaaga tcgtggtgaa tatgtccgcc gggcgcattt tccagatagc gatgcagata
    22021 atcccgatac ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga
    22081 aagaccgcgc agatcggcga tcccttgttc ggtgatgatc actttgacgc tgtgttcgct
    22141 gtgatcaaca tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga
    22201 cggagccata aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc
    22261 gttcatcaga tcgaccccgg ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc
    22321 caggccgacg ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat
    22381 ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc atattgttgt aaatcttgcg
    22441 cagggaatcg gccgagattg tcaggctgga ggcgctggcc ccgctgattt tgccggtttc
    22501 cagtaaatgc accaccgatt cctgtagcac ttccgaatac atcatgaacg gaggaatttc
    22561 cgggttttcc cccagacgcg ccattaccgc attattgata ttgcccacgc cactttgcag
    22621 cggcagaaat tccggcggaa tacgcccatg cgccatttcc tgcaataaga acgtgaccac
    22681 gttatcggca atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcggg
    22741 caagttggtt tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc
    22801 gacgcgatcc attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat
    22861 cacaatatct gccagttctg caacgcgcgg atcgtgatag tgattaagtt caatgatcac
    22921 tttcttcgct cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg
    22981 accatccggt gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc
    23041 gtaattgacc atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg
    23101 attgatcttt ttacgtaaac tggacgatgt ttgatatggc gcacgccagg aaacagcatc
    23161 ggcgtcagaa agtacatcgt cagcggcggc gctgattgac gcccccgtca gcaggcgaat
    23221 ttgatacggc tttttggcct catgctgttc gttagctctg cgggcaatcg cggcgggtag
    23281 ggctttcggc gaacccgctg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat
    23341 aatttctgcc gcttcatcgg cggtcatcct tgtccactga gtttccattt ttactccttc
    23401 gttcgcaggg attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc
    23461 ccttcctggt aatcttcgct gtcataaacc gcgcggcgca tcccctgaat acgttcaaat
    23521 tcatcggagt tcatggtgtg tgcttcgccc agtacacgca gctcttcttt gataacggca
    23581 atagctaacg gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt
    23641 tcttccactt ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt
    23701 ggcgaagcgg taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg ggtgaggttg
    23761 tgaatgccga ccaggttata cgggacgccg aggtttacag gcgtcattga gaaggttgag
    23821 gtactggcgg cgatgatcag atcggaactc atgatcattt caaatgcgcc accccaaaca
    23881 ctaccttcca ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga
    23941 cgcaatggat catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg
    24001 tgacctgcgg agaagacttt ggatccactt ggcgcccgca aaatgataca gcgaatttcc
    24061 ggccggttga gatcgcttaa cgcctgcata agattatcaa taaagacttt acttaaggca
    24121 ttaagttttc gtccatagtt aaactcaatg accgccactt tgttgatagt ggcaacgttc
    24181 acatactgat aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact
    24241 gagctgccgc aggccggtgc gcggtgagag cgtattgttt ttgactgcta aaagcgtctg
    24301 gcggtaatag cgatcgaaat cttcattcgc gaacaggtga ttcagtactt cttcttcggt
    24361 ctgcttacgc agccattcca tcgattgttg ttgccgcact tgttgtaaac gaccactggc
    24421 agttagcgcg gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc
    24481 cagtgcgcta caagtcagac cccgtggctg ccattcgtcg tatttacgtc gcagaatatg
    24541 cagggcactc tcgtacatat gccgggcaat ggcgacattg gtatggttat cgctatcgtc
    24601 tttgttgata acgagcagat cagccacttc catcagccct tttttaatgc cctgcagatc
    24661 atcgccgcca ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttccgt
    24721 ttccgactgc gcgacgccaa ccgtttcgac aatcactacg tcataacccg ccgcttcgca
    24781 taacagcatt aattcccgtg ctcgctgact ggcaccgccc agatgaccgg aggagggtac
    24841 cgggcgaata aacgccgcct cggcacgcgc caggtcattc atgcgggttt tatccccgag
    24901 aatgctaccg ccagtgaccg ggctgctggg atcgaccgca ataacagcaa cctttaatcc
    24961 ctcacgaatc aacaacatgc caaaggcttc aagaaaggta cttttccccg cgccgggtgt
    25021 gccggtaacg cccagtcgca gggcgttacc acagaacggc ataatggcgt caagcagctg
    25081 tgtacttagc gcctgatgac gtgggtgacg gctttccacc agcatcatgg cttgggcgag
    25141 cgtggcacgc tcaccctgac gtaagcggcg aatactttct gccagcgtgg cttcattaat
    25201 catgatgctg gcttatcaga ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg
    25261 gaccataaat cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga
    25321 cgccacccgc gaccacgcag atatcttcgc gtccccattt tttcagcgct tcgaccagtt
    25381 ccgggatcag cgttttatga ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt
    25441 tttctacggc caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt
    25501 cgaaaccgag atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgtccatcct
    25561 ggcccatctt agcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg
    25621 tttgcgcaac aatggcatcg aactcggagg ccgatttctc agactgatga tagctttgcg
    25681 caatcacgcc ggtaacacac tggcaccaga taacggtcga aagcgacttc cagcgcatcg
    25741 gaaatttcac ccagggtggc gcgaacgcga gcggcattaa cagcggcagc cagcaggttt
    25801 tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg cggttacggc ggcatcatca
    25861 cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt tacgcaccat cacgttgtcg
    25921 atctcaagta catcggtttc gtcttcgtga tccagtttgt acttgttgac accaacgatg
    25981 acacgcttgc cctggtcgat cagcgactgt tcgcgcgctg aggcctcttc gatcattcgt
    26041 tttggcagac ctgcttcgat cgctttcgcc atgccaccgg cttcgtcgat ctgttggata
    26101 atagctctgg cttgtttgac gatttgatcg gtcagcgact caatgtaata ggatccggcc
    26161 agtggatcga cggtgtggca gagttctgat tcttcctgga tgatgatctg ggtgttgcgg
    26221 gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc
    26281 agtgactgag taccgcccag cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg
    26341 ttatacggat cctgttcagt caggctccag cctgaggtct ggcagtgggt acgcagcgcc
    26401 agtgatttcg gatcctgtgc gccaaatcca ctgaccgctt cgctccataa ataacgtgcc
    26461 gcacgcaaca tggcgacgtt cataaacaga tccatgccga taccgaagaa taacgacagg
    26521 cgaggagcga agtcatcaat tttcagtccg gcagagattg ctgctttgat gtactcaatc
    26581 ccatcggcga gcgtaaatgc tacctgctgc acgcagttgg cacccgcttc ccccatgtgg
    26641 taaccgctga tactgatggt attaaatcgc ggcatgttgc cggaacacca ggcgatgatg
    26701 tcggcgataa tgcgcattga cggttttggt gggtaaatat aggtgttgcg gcagaggtac
    26761 tctttgagaa tatcgttttg aatggtgccg gtcagtttat caggtgtaac accttgctct
    26821 tctgcggcga cgatataaaa cgccagtact ggtagcactg cgccattcat ggtcatcgaa
    26881 accgacattt tatccagcgg gatctggtcg aacaggactt tcatatcttc cacggtgtcg
    26941 atagcgacgc ccgctttgcc gacgtcaccc gccacgcgtg ggttatcgga gtcgtagcca
    27001 cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt gcccggcggc caggttacgg
    27061 cgataaaaag cgttggactc ttttgctgtt gaaaaaccag catactgacg gatggtccac
    27121 ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt agggcggcaa accaggaagg
    27181 gtacctgtca cctccagatt atcgagatcg gcttcggtat acagcggctt gatggcgatc
    27241 ccttccgcgg tttgctgaac cagcgagtcg acagttttct cccgacggct caattccttg
    27301 ttggcaagct gttgccactc ctgctcgtta gacattattt gctccgtaaa acgcaatcaa
    27361 aatgagggta tttgcgctta cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa
    27421 tgcattcaga cgtcgttttt tgccgcctga gatttgtcta ttttgtgatg tgattcaagg
    27481 aatacaaaaa gaagtgagga caacgcctga tatgtgctat caggcattat ttgatggatt
    27541 aatcctgacg aaggactttg tgaccataat cgagtaacgc atcagtgact ttacgcatca
    27601 tgcggctttc aggagcaaag cggtgccagt agagcatccg tcgttgaaat agcccaggcg
    27661 ttaagtcaat cagttcaccg ctggccagct ctttctcgat ttgcaggtgc gggatcatac
    27721 agcaggtggt gccctggcga gcaagttgta cgaacgcttc tgaagaatta acgatatggc
    27781 agggtacgct gccaggagga agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt
    27841 caagatggtc aaacgcgacc actggcgctt tcaataatgc cgaacgcgtt acgccgttag
    27901 gaaaatattt ttcggcaaac ggttttgagc tgacgaacag atagtcgagc gcaccgagtt
    27961 tatcgacaag acaactcggc agcgcctgat gttgaatact caccgcgccg accacttcgc
    28021 cgcggcgcag acgttcctga gtacgggttt catcttctac ctgcaagttg aggcggatag
    28081 gcgaatcagc caacacagga gccagtgccg gaagcaacca cgtcgccaga ctgtcggcgt
    28141 tgaccgccag tgaaagcagc agcggagtcg aaccggtttg ttcatcgccc agccactctt
    28201 cttccagcaa ctccacttgg cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc
    28261 gcggcggtac ggtacgcacc aacagcggct gcccgaacat attttccagt tgcttaatgc
    28321 gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt
    28381 cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg
    28441 tctgatcctg gtaaatgctt tttcctgcac tatgacacaa ttttgtgtca ggttgcatat
    28501 accgaagccg tcggcgggta acatttgtat caatggttaa acgcgcagct taaaagatca
    28561 gggatttgca ctattgcatt tcgcttagcg acttcgcgcc ctggttatgt tgaatggcgt
    28621 ggcgttattg cctctgttcg caccgtggag cggcataccc agaccgttgt atgctttatt
    28681 tgcggggcga aaggggatgc ccgccattgc gcgatatttt atggatgagt taaccacccg
    28741 gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa tttcatacca
    28801 caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg gcggcacttc
    28861 agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc gcacacttta
    28921 ttgacgcgct cggcacgatg aaaggccaga ttgaaggggc cgtttccagt tcagatgctt
    28981 ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa gtcgacagcc
    29041 ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg
    29101 gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta
    29161 ttgccgacgc ctccaagcag gttgatattc tgggtaaatt cccgctgcca gtagaagtta
    29221 tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa actgggcggt cgtccggaat
    29281 accgtcaggg cgtggtgacc gataatggca acgtgatcct cgacgtccac ggcatggaaa
    29341 tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg
    29401 gcttgtttgc taaccgtggc gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa
    29461 ccattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc ttcattaaat
    29521 ttggtgacat gtgtcacgct tttagcaggc aattgtcgat tgctctaaat aaatcctcta
    29581 aaccagcata ttcatccaag aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa
    29641 acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc aaatccgcac
    29701 acaacatttc aaaagacagg attgggtaaa tggcaaaggt atcgctggag aaagacaaga
    29761 ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg
    29821 gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta aaagaatcca
    29881 tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa gacgtgatca
    29941 acgccgcaga aaaactggtc gctattggct gtttctgtat cggaacaaac caggttgatc
    30001 tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca aatacgcgct
    30061 ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca
    30121 atgctaaagc gcaccgtggc gtgtggaaca aactggcggc gggttctttt gaagcgcgcg
    30181 gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc attctggctg
    30241 aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg ctgggcaacg
    30301 ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg agtctgcatg
    30361 taccagagaa tccgtccacc aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc
    30421 ccggctcgct gctgattaat gcctcgcgcg gtacagtggt ggatattccg gcgctgtgtg
    30481 atgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg acggaaccgg
    30541 cgaccaatag cgatccattt acctctccac tgtgtgaatt cgacaacgtc cttctgacgc
    30601 cacacattgg cggttcgact caggaagcgc aggagaatat cggcctggaa gttgcgggta
    30661 aattgatcaa gtattctgac aatggctcaa cgctctctgc ggtgaacttc ccggaagtct
    30721 cgctgccact gcatggtggt cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc
    30781 taactgcgct gaacaaaatc ttcgccgagc agggcgtcaa catcgccgcg caatatctac
    30841 aaacttccgc ccagatgggt tatgtagtta ttgatattga agctgacgaa gacgttgccg
    30901 aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt ctgctgtact
    30961 aattcccctt ctctgaaaat taacgggcag gtcactgact tgcccgtttt tttatccctt
    31021 ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga aaataaatca
    31081 aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcggg acagcatggg
    31141 gctgtaacgg taaagccctc accgaagcga gggcttaaag gagaagggtt atgatgcgac
    31201 ttgtcatcat actgattgta ctgttactga taagtttcgg agcttattaa cagtcagtct
    31261 caggggagga gtcatcctcc cttaccctta ctcactaaat taggtcaaag aatcaacgat
    31321 gtcaatcagg gcgatgcggg gtgcatcgcc ctgaaccgaa ctttttgata gccacttcat
    31381 cgcccacgtt tactgccgta atagatcctt tttcccaaaa attatctctg ttttgcatgg
    31441 ggtttttcga gcacgctcga agaaaagatt taaaggtaga tcaatgcgtt gtggttattt
    31501 aaagagagct tacgatagct gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc
    31561 gagggcttga aggagaaggg ttatgatgcg acttgccatc atactgattg tactgttact
    31621 cataagtttc ccagcttatt aacagtcagt cacaggggag gagcaatcct cccttaccct
    31681 tactcactaa attaggttaa agaatcaacg atgtcaatca gggcgatgca ggtgtatcgc
    31741 ccttaccact cccagacttt cgacggtgta accaccgcag gaagagggat atcccactct
    31801 tcaacgggga gtttttccac caactgacaa tcatgcgcat aacccaccgg ttgcgtttta
    31861 tagtgctgcc agttttgtaa ggtccgatca taaaaaccac cgcccattcc caggcgctga
    31921 ccgtactcat caaaggcgac cagcggtgtg atcagcacgt ctaatcggga aaggggtagc
    31981 acgtcacgca catccagttt tggctcatgg atcttcaacc tgttcatcac cagttcgctt
    32041 tgcggatggt aattcaggaa cagcaaatta ccggcactaa agggatgcaa aactggaaga
    32101 tatacgcgct taccggcgcg ccagagttgt tctattagcg gctgggtgtc gagttcacca
    32161 tcaaaagaga ggaatacagc gaccgtatgc gccatcacca ccgggggata agtcatcatc
    32221 cgggtagcgg cttgttgacc catttccagc tgttgttccg gcgttaacgc acgacgacgt
    32281 tgccgaatca ttttgcggat ttcttgtcgg gataatgtca gtgggagctc tggtagttgc
    32341 gtcatggctg gtgccagata agaagggaat ctccgagatg ccgccgcagg ctgtaaccct
    32401 tgaacccttg gttcaaggtg aatgtgtcgt cgcagtttta aggcttctcg gacgaaccga
    32461 gcatgctcac caaccgcgga gcgccacatt cttgtggtat gaaatatcgg ctcaggggac
    32521 tggcccgctt gcgaacatct cagagaaatt ttgtcttcac ggttactcta ccacagtaaa
    32581 ccgaaaagtg tcattcaaag ttttggttag ttttttcggt gatgcgacct tgttcaagta
    32641 acgcttgttc tatggtctgc tgcagcatcc gaatacgctg ttccatactt gccgcgtagt
    32701 cacgagtctt tgctttttct tgcgctaact catagctgat attcaatgcg gcaatgaaga
    32761 ccaactgttc agtatttgtg actctagtgc gttctttcag atcttgcaac cgttggttca
    32821 gatcgtccgc tgcctgattc aacgcatccc tttggtcagg cgggcagttc acacgcagtg
    32881 aacggccaaa aatttggata tcgacgggtt gtgcagacat gccaccttcc tgctgattga
    32941 ctgcgctgcc ttcgtcttta gacccatggt ctgcgaaggg gcgacactat agctaccctg
    33001 atgagaagag acaagccctt ttctggtcca ccagggccca aagtggtagc atatcatgaa
    33061 tattcctccc tttgacgacg aatgcttatg tctatacaga acgaaatgcc tggttacaac
    33121 gaaatgaacc agtatctgaa ccaacaaggg acgggtctga cccctgccga gatgcatggt
    33181 ttaatcagtg ggatgatatg tggcggtaac gatgacagct catggctgcc gctacttcac
    33241 gacctgacga acgaaggcat ggctttcggt catgagctgg cacaggcact gcgtaaaatg
    33301 cactctgcca ccagcgatgc cctgcaggat gacggcttcc tttttcagct ttatctgcct
    33361 gatggcgatg atgtcagcgt tttcgatcgg gctgatgcac tggcaggttg ggtcaatcac
    33421 ttcctgcttg gtcttggcgt tacgcaaccg aagctggaca aagtgaccgg cgaaaccggt
    33481 gaagccatcg acgatctgcg taacatcgcg cagctgggct acgacgaaga cgaagatcag
    33541 gaagagcttg aaatgtcgct tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta
    33601 tgccacgaca cctttactca tccgcaaccg accgcgccag aagtacaaaa accgactcta
    33661 cactaaaaac aaaaaacgta aggagagtgt tatgagtgag atatcccggc aagagtttca
    33721 gcgtcgccgt caggccctgg tggagcaaat gcaacccggc agcgccgcgc tgatttttgc
    33781 tgcaccagaa gtaacacgta gcgccgacag cgaatacccc tatcgtcaga acagtgactt
    33841 ctggtacttc accggcttta acgaaccgga agcggtgctg gtgctgatta aaagcgatga
    33901 cactcataac cacagcgttc tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt
    33961 tggccgtcgc ttaggccagg atgccgcgcc agagaaactg ggcgttgacc gcgcactggc
    34021 attcagcgaa atcaatcagc aactttatca actacttaac ggcctggatg tggtttacca
    34081 tgcccaggac gaatatgcat atgctgatga aatcgtgaac agtgcgctgg aaaaactgcg
    34141 taaaggttcg cggcaaaatc tcaccgcacc ggcaacgatg atcgactggc gtcctgttgt
    34201 tcatgaaatg cgcctgttca aatcgccaga agagattgcc gtactccgcc gcgcgggaga
    34261 aatcaccgcc atggcacata cacgggcgat ggaaaaatgc cgtccgggaa tgttcgagta
    34321 ccatctggaa ggcgaaattc accacgaatt taaccgccac ggtgcgcgct atccttccta
    34381 taacaccatt gtaggcagcg gtgaaaacgg ctgcattctg cactacaccg aaaacgagag
    34441 tgaactgcgc gacggtgacc tggtgttgat tgacgcgggt tgtgaataca aaggttacgc
    34501 gggcgatatt acccgcacct tcccggtcaa cggcaaattc acccaggccc agcgtgaaat
    34561 ctacgacatt gtgctggagt ctctcgaaac cagcctgcgc ctgtatcgtc cgggaacttc
    34621 cattctggaa gtcactggtg aagtggtgcg catcatggtt agcggcctgg taaaactcgg
    34681 catcctgaaa ggtaatgttg atgaactgat cgctcagaac gcccatcgtc ctttctttat
    34741 gcatggcctt agccactggt taggactgga tgtccatgac gttggcgttt atggtcagga
    34801 tcgctcgcgc attctggaac cggggatggt actgaccgta gagcctggtc tgtatatcgc
    34861 gccggatgcg gatgtgccag aacaatatcg cggtatcggc attcgtattg aagacgacat
    34921 tgtgattacc gaaaccggca acgaaaacct caccgccagc gtggtgaaaa agccggaaga
    34981 aatcgaagcg ttgatggctg ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc
    35041 atggcgggcg cgacgctggc gctggctatt tcccggttaa gtcacggggc gctgccggta
    35101 catttgattg aagcgactgc gccagagtca catgctcatc cgggctttga tggacgagcg
    35161 atagcgctgg cggcgggtac ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg
    35221 gctgattgcg caaccgccat cactaccgtg catgtcagcg atcgtggtca tgctggattc
    35281 gtcaccctcg tcgcagaaga ttaccaactg gcggcgctgg ggcaggttgt cgaattgcat
    35341 aatgtcgggc aacggctgtt tgcattgctg cgaaaagcac ctggcgtaat gctgcattgc
    35401 cccgatcgcg tagctaacgt tgcccgtact cagagtcacg ttgaagtgac gctggagagc
    35461 ggcgagatgc tgacgggccg cgtgctgata gccgctgatg gcacccattc agcgttagcc
    35521 accgcgtgcg gcgttgattg gcagcaggag ccttacgaac aactggcagt gattgccaac
    35581 gttgctactt ctgttgcgca cgaaggccgc gcttttgaac gcttcacgca acatggcccg
    35641 ctggcgatgt tgccgatgtc tgacggacgc tgttcgctgg tctggtgtca tccactggaa
    35701 cggcgcgaag aggtgctgtc gtggagcgac gagaagtttt gccgtgaact ccagtctgcc
    35761 tttggctggc gactggggca aattacccac gccggtaaac gcagtgctta tccgctggca
    35821 ttaacccgcg ccgccagacc gattacccat cgcactgtgc tggtgggcaa tgcggcgcaa
    35881 actctgcacc cgattgccgg gcaagggttt aacctcggta tgcgagatgt catgagcctt
    35941 gcggaaaccc tgactcaggc gcatgagcgc ggagaagaca tcggtgatta cggcatattg
    36001 tgccgttatc agcagcgtcg acagagcgat cgcgaagcaa ccatcggcgt cacggacagc
    36061 ctggtacatc tttttgccaa ccgttgggca ccgctggttg tcgggcgcaa catcggactg
    36121 atgacgatgg aattattcac cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg
    36181 gtggcgcgtt gaaggcggtt ttaaggaata aacaatgcaa agtgttgatg tagccattgt
    36241 tggcggcggc atggtggggc tggcggttgc ctgcggctta caggggagcg gcttacgcgt
    36301 tgccgtactg gagcagcgcg taccggaacc tctggcggta gatgcactac cacaactgcg
    36361 cgtttcggct atcaatgccg ccagcgaaaa attactcacc cgtcttggcg tctggcagga
    36421 cattctcgct cgtagggcta gttgctatca cggcatggaa gtgtgggaca aagacagttt
    36481 tggtcacatc tcgtttgacg atcaaagcat gggctatagc catcttgggc acatcgttga
    36541 aaactcagtg attcactacg cgctgtggaa caaagcgcag cagtcgtcag atattactct
    36601 gttggccccc gcagaattac agcaggtcgc ctggggagaa aatgaaacct tcctgacgct
    36661 gaaagatggc agcatgttaa cggcgcgtct ggtgattggc gcggacggtg ctaattcctg
    36721 gttgcgcaac aaagcagata ttccgctgac tttctgggat tatcagcatc acgcgctggt
    36781 agcgaccatt cgcacggaag aaccgcatga tgcggtggcg cggcaggttt tccatggcga
    36841 aggcattctg gcctttttac cgcttagcga tccgcatctt tgctcgattg tctggtcact
    36901 gtcgccagag gaagcgcagc ggatgcagca ggcaagtgaa gacgaattta atcgcgcgtt
    36961 aaatatcgct tttgataatc gccttggctt atgcaaggtt gagagcgcgc gtcaggtgtt
    37021 cccgctgacg gggcgttatg cgcgccagtt tgccgcacac cgtctggcgc tggtgggcga
    37081 cgccgcgcat accattcacc cactggcggg gcagggcgtt aatctcggat ttatggacgc
    37141 cgcagagctg attgctgaac tgaaacggtt gcatcgtcag ggtaaagaca tcgggcagta
    37201 catttacctg cgccgctatg aacgtagccg caagcacagc gcggcgctga tgctggctgg
    37261 tatgcagggt tttcgcgatc tcttttccgg cgctaatccg gcgaaaaaac tgctgcgtga
    37321 tattggtttg aaactggccg acacgcttcc tggcgttaag ccgcaactta tccgccaggc
    37381 aatgggatta aacgatttgc ctgaatggct gcgttaaaaa tttctcctct gttgtttatt
    37441 tgatacccat cacactttca tctcccggtt ttttcgccgg gagattttcc tcatttgaaa
    37501 taaactaatt tcacctccgt tttcgcatta tattttctaa tgccatgatt ttttgattta
    37561 gtgttttttg acattttttt agctcttaat attgtcttat ccaaattgac tttctcatca
    37621 catcatcttt gtatagaaat tggtgtattt tttggttttt tattctgtcg cgatttttgc
    37681 attttttaac cataagctaa tgtgatgatc aattttacct tatggttaac agtctgtttc
    37741 ggtggtaagt tcaggcaaaa gagaacgatt gcgttgggga ccgggagtgg ctccgatgct
    37801 gggttttgtg gtgataattt caccatgaaa aagttgtcag ccccgcttat tcaatgagga
    37861 caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt
    37921 ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc
    37981 ggtacgtacc gatgccggaa tgtttgatgt gtcgcatatg accatcgtcg atctccgcgg
    38041 cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa
    38101 aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgatct
    38161 catcgtctac tactttactg aagatttctt tcgcctcgtt gttaactccg ccacccgcga
    38221 aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg
    38281 tgatgacctt tccatgattg ccgtgcaagg gccgaatgct caggcaaaag ctgccacact
    38341 gtttaatgat gctcagcgtc aggcggtgga agggatgaaa ccgttctttg gcgtgcaggt
    38401 gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg aaattgcgct
    38461 gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagcgggcg ttaagccctg
    38521 cggtctgggg gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt atggtcagga
    38581 gatggacgaa actatttctc ctttagccgc caacatgggc tggactatcg cctgggaacc
    38641 gacagatcgt gactttatcg gtcgtgaagc cctggaagtg cagcgtgagc atggtacaga
    38701 aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg
    38761 ctttaccgat gcgcagggca accagcatga aggcattatc accagcggta ctttctcccc
    38821 gacgctgggt tacagcattg cgctggcgcg cgtgccggaa ggtattggcg aaacggcgat
    38881 tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgaca aaacctgttt ttgtgcgtaa
    38941 cggcaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca
    39001 gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttacacc
    39061 gttggtatta ccgaacatgc tcaggagctg ttaggcgata tggtgtttgt tgacctgccg
    39121 gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc ggtaaaagcg
    39181 gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc
    39241 gattccccgg aactggtgaa cagcgaaccg tatgcaggcg gctggatctt taaaatcaaa
    39301 gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta
    39361 gaagatgagt aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaaattta
    39421 tgcctcaccc tcactctctt cgtaaggaga gaggttcaca attcactgca cgtttcagga
    39481 accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt ttattgaacg
    39541 ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg gtgcacaatc
    39601 gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga caccaccgca
    39661 ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta ttgccagtcg
    39721 caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc taccgccggt
    39781 tatcctgcgt aacatgctgg aaaatccggg ctggtatacc gcgtacactc cgtatcaacc
    39841 tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa cgctggattt
    39901 gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg ccgccgaagc
    39961 aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc
    40021 caatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga
    40081 agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt
    40141 acagcaggta ggcactaccg gtgaaattca cgactacact gcgcttatta gcgaactgaa
    40201 atcacgcaaa attgtggtca gcgttgccgc cgatattatg gcgctggtgc tgttaactgc
    40261 gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg gcgtgccgat
    40321 gggctacggt ggcccacacg cggcattctt tgcggcgaaa gatgaataca aacgctcaat
    40381 gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc
    40441 gatgcagact cgcgagcaac atattcgtcg tgagaaagcg aactccaaca tttgtacttc
    40501 ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc cggttggcct
    40561 gaaacgtatc gctaaccgca ttcaccgtct gaccgatatt ctggcggcgg gcctgcaaca
    40621 aaaaggtctg aaactgcgcc atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga
    40681 caaagcgggc gtactgacgc gtgccgaagc ggctgaaatc aacctgcgta gcgatatcct
    40741 gaacgccgtg gggatcaccc ttgatgaaac caccacgcgt gaaaacgtaa tgcagctttt
    40801 cagtgtgctg ctgggcgata accacgggct ggagatcgac acgctggaca aagacgtggc
    40861 tcacgacagc cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc
    40921 ggtgtttaat cgctatcaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg
    40981 taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaactgaa
    41041 cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc acccgttttg
    41101 cccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg actggctggt
    41161 gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac agggcgaata
    41221 cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat
    41281 ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata tggcaggaat
    41341 gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg atctgcgcgc
    41401 gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc cttctaccca
    41461 cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca
    41521 ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat
    41581 tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc acggcggtgg
    41641 tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca
    41701 tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt
    41761 cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg
    41821 gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca
    41881 ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct
    41941 cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct
    42001 gatcgactac ggtttccacg cgccgacgat gtcgttcccg gtggcgggta cgctgatggt
    42061 tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc
    42121 tatccgcgca gaaattgacc aggtgaaagc cggtgtctgg ccgctggaag ataacccgct
    42181 ggtgaacgcg ccgcacattc agagcgaact ggtcgccgag tgggcgcatc cgtacagccg
    42241 tgaagttgcg gtattcccgg caggcgtggc agacaaatac tggccgacgg tgaaacgtct
    42301 ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca
    42361 gtaattcact gattcgagta ttttctaaag gcgcttcggc gcctttttag tcagatgaca
    42421 aagtacaaaa gtgctcagac agtcccctcg cccctttggg gagagggtta gggtgagggg
    42481 aacaggccgg cactggcgcg aatatttacc ctcaccccga ccctctccct gaaagggcga
    42541 gggggaaaag cgtgccaaca ttgaagattg agccagtttg ttagcaatct caaagatacg
    42601 tcaacgaatt aatttttctc ggaaaaacaa atggctatag cacttgtgac cggtggcagt
    42661 tgcggcatcg ggcgggcaac tgcattactg ttggcgcaag aagggtatac ggtggcggtt
    42721 aattatcagc aaaacctcca cgcggcgcag gaagtgatga acttaataat gcaagccggt
    42781 ggcaaagcat tcgtgctcca ggcggatatc agcgacgaaa accaggtcgt tgcgatgttt
    42841 acagcaatcg atcagcacga tgaaccgcta gcagcgctgg tcaataacgc cgggatcttg
    42901 tttacccagt gcaccgttga aaaccttacc gcagagcgaa tcaaccgagt actttccacc
    42961 aacgtgacgg gatattttct ctgctgccgc gaggcggtaa aacgcatggc gcttaaaaat
    43021 ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg cctcacgatt gggttcgcca
    43081 ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg atacgttaac caccggacta
    43141 tcgctggaag tcgccgcgca ggggatccgc gttaactgcg tgcggccagg gtttatttat
    43201 accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc
    43261 cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg ccattgtctg gctactaagt
    43321 gataaagcct cttacgtcac gggaagtttt atcgatttgg cgggcgggaa ataaaatagg
    43381 gaagttgtct gaccggatgc aacaagtatt gcatccggta cttcatcgac ttaaagcttc
    43441 tcgccgttgc tggcaatcac ctctttgtac cagttaaagc tcttcttacg tgaacgcgac
    43501 atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg tttgctgtac
    43561 tgcccggtag tgaatgacac gcagtcgatg cagccccacg gtgtgtagcc catcagatcc
    43621 acaccatcgt aagtcactgc ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg
    43681 cggtagtcgt cgttgatgct gccatcttct tccactttgt cgtaagcgcc aaaaccgttt
    43741 tcgacaataa acagcggcct ctgataacgc tcatacagtt cgcaaagggc atagcgcaga
    43801 cctactggat caatctgcca gccccagtca gacgctttaa catacgggtt cggtacgctg
    43861 ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg ccttcactgc gttggtcatg
    43921 taatagctga aaccaagata atcgcaggtg ccttcgcgca gtacatcgag atcgccgtct
    43981 tccattttga tattaaatcc gcgacgttcc cactcgttca atacatagga cgggtaatag
    44041 ccgcatagct gcacatcggt aaagacgtag cgttcgcgca tcgactcctg agcgaacatc
    44101 acatcgtccg ggttacatga gtagggatag agcggcacca tcgccagcat acagccgact
    44161 ttcatctccg ggttaatgcg acgcgcggct ttcactgcca gggcgctggc gacaaactgg
    44221 tgatgcagca cctggtacat cgtctcttcc gggttgtcat gctcggtata caccacgccg
    44281 gagcagcagt aaccaaacag cggcgcacgc cagttacgct ggttgtttat ttcgttgaag
    44341 gtcatccagt atttgacttt atgcttgtag cgttcgaata ccacttccgc gaaacggaca
    44401 aagaaatcaa cgactttacg gttggtccag ctaccgtatt gctgcaccag atgcagcggc
    44461 atttcaaagt gggagagggt tatcaccggt tcgatgttgt atttcagcag ttcatcgaac
    44521 agagagtcgt aaaacttcag cccttcttcg tttggctgag cttcatcacc ttttggaaaa
    44581 atacgcgtcc aagcgatgga agtccgaaaa catttgaagc acatttcggc aaatagcttg
    44641 atgtcttcct tgtagtgacc ataaaaatca acggcttcat ggtttggata gtattttcct
    44701 ggcaagactt ctttggtgat ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca
    44761 caaatgctcg gtcctttgcc gcctttgttc cagccgcctt cgacctgatg agcggcaacg
    44821 gcgccacccc ataagaaatc tttcggtaag gtgagttttt tcactatcat aggctccaga
    44881 tcagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt tttttcaacg
    44941 tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc
    45001 gcacttcaaa acgggtgatg tgcttcgtgt cggacgtttt gaagatgacg gttatttttg
    45061 cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat acactgacgg aaaaacatgc
    45121 agagcaggaa aatatgaccc tgcctgaact gaaaaaggtc attgctgaca tctatcccgg
    45181 tcagacacaa ttttatatga ttgaatttaa atgtctttaa attgaactca cgcctgttag
    45241 ttgaaatttc gcatttaatt tcatgatttt taggagatta gtctcaaatc agaatttatt
    45301 ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta tacatgtgcg
    45361 tgtacgtttc tctggagtaa gttatggttc agaagcccct cattaagcag ggatattcgc
    45421 tggcagagga aattgccaac agcgtcagtc acggcattgg gctggtgttt ggtatcgttg
    45481 ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca gcgataacca
    45541 gttacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg ctctatcacg
    45601 ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc gctatttacc
    45661 tgttgattgc cggaacctac acgccgtttt tgctggtggg gctggattct ccgttagcgc
    45721 gcgggttgat gattgttatc tggagcctgg cattgctggg gattctgttc aaactgacca
    45781 tcgcgcaccg attcaaaatt ttatctctgg tgacttatct ggcgatgggc tggctgtcgc
    45841 tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc ttactggcgg
    45901 taggcggcgt ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc attccataca
    45961 accatgccat ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt ctggcgatct
    46021 atttgtatat tgggcaggcg taatgggtat gcctttgtag gcctgataag acgcgtcagc
    46081 gtcgcatcag gcatcggagc gcaaccgccg gatgcggcgt gaacgcctta tccgatctac
    46141 gggcctcgtc cctgttgtga taaaggcatc aaatcccaga catgataaga cgcgtcagcg
    46201 tcgcatcagg cgataaatgc ctgatgcgat acagagattt actcttcgag cgaatacggc
    46261 agcggctcga tatgcagtgt attcgcatcg tcgcgtacgc ggaagatgct gtccggttcc
    46321 atatcgttat tcatgacgac ctgtaccacg acctgaccat cttccagttt taccgcagcc
    46381 agcgccgtac cggtacgacg ccagttctcg cccattttca gctctaagtc ttcaccagct
    46441 tccggcagtc ggctggcact acctgccagc aaccagagcg cgcgtttgtt ggcaccacgg
    46501 aattttgctc gcgccaccat ctcttgtccg gtataacagc ctttcttaaa gctgataccg
    46561 cccagcgcct ggagattggt cgcctgtggg ataaactgcc cgctgttggc ggcatcaatc
    46621 accgggaaac cagcttcaat gtttaatgcc agccactgtt ggctattgtt cagttccgct
    46681 tcaccgcgca gtttatcggt cagcatatta gcagtagctt catcggttac gatcaggaaa
    46741 cgttctgccg ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttgac tacctgtttt
    46801 tctttcgaag gcagttcgct aaagagattt gccagcgcgg cgcgcgcctg aaaaccggca
    46861 acaccaagca gcacacgctc gtcgtctggc gcgatggtca ctttagagaa taccgcatat
    46921 tttttcagtt cagtcagctg cggttcacgc acgctgcgcc gttcaatcca tgcaaagcca
    46981 tcgccgtcgc ggaacagacg taaattgctc cacattttac ctttggcgtc gcaatgggcg
    47041 gcgagcaggt gctgatcttc tgccatctgg ctgacatctg ctgtcacctg accctgcata
    47101 tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag cgtcatcagc
    47161 gtcagcggta aacgggcaga agccgtaggc tgacgtggag gaaaaggtgt aaaagccata
    47221 atagagtcct gagttgctta acggcgtatt aatgactaat ggtaaaagag ctatgaggca
    47281 atgcaagtgc tttcactacg cgatctgtgc cttctgcgag gcgactgcca aaaaaatgaa
    47341 gcccttgaat tgttgtgaat taatttcgcg atcgcgatgg tgtttactga tattcccgta
    47401 aaagaagtcg aaaaacacgt tacaatagcc gggtactgga ttttcgtgag acaggaagaa
    47461 gatggacatt aacaacaaag cccgcattca ttgggcatgc cgccgtggta tgcgcgaact
    47521 cgatatttca atcatgccgt ttttcgaaca tgagtacgac agcttaagcg atgacgaaaa
    47581 acgcatcttt attcgtctgc tggaatgtga cgatccggac ctgtttaact ggctgatgaa
    47641 tcacggtaaa ccggccgatg cagaactgga aataatggtc cgactcatcc agacacggaa
    47701 ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca cagtggcttt
    47761 ccttgctgat tcatgggctg gttgccgctg ttattttact catgccctgg ccgctcagtt
    47821 acaccccgtt atggatggtg ttactttcgc tggtggtatt tgattgcgtt cgcagccagc
    47881 ggcgtattaa tgctcgccag ggggaaattc gcttgttgat ggacgggcgt ttgcgttggc
    47941 aagggcagga gtggagcatc gtcaaagcac cgtggatgat taagagcggc atgatgctgc
    48001 gtttacgttc tgatggcggt aaacggcaac atttatggct ggcagccgac agcatggacg
    48061 aagccgaatg gcgggattta cggcggattt tgttgcaaca agagacgcaa agataaagat
    48121 gacggcaaag catatgcgct aagccgtcag tgagatcagg cgtaatgctc tgccatttcg
    48181 ttgaggattt gctcgcacca gctctgaata cgctcgtcgc taaggtcata ctggttagtt
    48241 tcatccagcg ccagacccac gaacagttgc ccgtcagcaa tcaccggttt cgggctggta
    48301 aattcatatc cttccgttgg ccagtagccg acgaacttca cgcctttggt cgagagtttg
    48361 tcatgcagca taccgagcgc atcgaggaac cactcgccgt atcccagttg atcgccaagc
    48421 ccatacagcg caacaatttt accttcaagg ttcaggtcgt cgagctgatc ccagacggct
    48481 tcccagtctt cctggatttc accaaaatcc caggtcggga tacccagaat gagcacatcg
    48541 tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac cagttctggg
    48601 ccgataatat cgcggatttt ttctgccgcc atttcggtgt aacaggtgct ggaaccgtaa
    48661 aaaagaccca tattcataac gtaagcttct cgattctgct gtggcttttg cagctagtgt
    48721 atcagaatcg ctttagtcac ggcacaataa ggcataatgg atcaaatgag tgaccataag
    48781 gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt tcttgatgct ctgtggctgg
    48841 aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca atgatggtgg
    48901 agtggttgca tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat ttgcaggcat
    48961 tactggcaga acggctggag ggcgggtata aagccaccag ctcagcgcgt ttgctgagtg
    49021 cagtgcgccg attgttccag tatctttatc gcgaaaagtt tcgtgaagac gatcccagtg
    49081 cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc gaagcgcagg
    49141 tcgaacgttt attacaggcg ccattaattg atcagccact ggagctacgc gataaagcca
    49201 tgcttgaagt gttgtatgct accgggctgc gtgtctctga actggtcggg ctgacaatga
    49261 gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac aaagagcgcc
    49321 tggtgccgtt aggggaagag gcggtttact ggctggaaac ctatctggaa catgggcgtc
    49381 cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt gcgcagcaga
    49441 tgacgcgaca gaccttctgg caccgcatta aacattatgc cgtgctggcg ggtatcgaca
    49501 gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc cactcattta ttaaatcatg
    49561 gtgcggattt acgcgtggtg cagatgctac tgggccacag cgatctttcc accacgcaaa
    49621 tttatacgca tgtcgctacc gagcgtctgc ggcaacttca tcaacagcat cacccgcggg
    49681 cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg ttttatgttg tttactttgt
    49741 tagcggcgtt ttcaggcttt gctcaggctg atgacgcggc aattcaacaa acgttagcca
    49801 aaatgggcat caaaagcagc gatattcagc ccgcgcccgt agctggcatg aagacagttc
    49861 tgactaacag cggtgtgttg tacatcaccg atgatggtaa acatatcatt caggggccaa
    49921 tgtatgacgt tagtggcacg gctccggtca atgtcaccaa taagatgctg ttaaagcagt
    49981 tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa cacgtcatca
    50041 ccgtgtttac tgatattacc tgtggttact gccacaaact gcatgagcaa atggcagact
    50101 ataacgcgct ggggatcacc gtgcgttatc ttgctttccc gcgccagggg ctggacagcg
    50161 atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga taaaaacaaa gcgtttgatg
    50221 atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga cgtggatatt gccgaccatt
    50281 acgcacttgg cgtccagctt ggcgttagcg gtactccggc agttgtgctg agcaatggca
    50341 cacttgttcc gggttaccag ccgccgaaag acatgaaaga attcctcgac gaacaccaaa
    50401 aaatgaccag cggtaaataa ttcgcgtgaa acaacagata caacttcgtc gccgtgaagt
    50461 cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg ctgcgccgtt tatacgccag
    50521 ccggggagta cgcagtgcgc aagaactgga acgcagtgtt aaaggtatgt tgccctggca
    50581 gcaactaagc ggtgtcgaaa aggccgttga gatcctttac aacgcttttc gcgaaggaac
    50641 gcggattatt gtggtcggtg atttcgacgc cgacggcgcg accagcacgg ctctaagcgt
    50701 gctggcgatg cgctcgcttg gttgcagcaa tatcgactac ctggtaccaa accgtttcga
    50761 agacggttac ggcttaagcc cggaagtggt cgatcaggcc catgcccgtg gcgcgcagtt
    50821 aattgtcacg gtggataacg gtatttcctc ccatgcggga gttgagcacg ctcgctcgtt
    50881 gggcatcccg gttattgtta ccgatcacca tttgccgggc gacacattac ccgcagcgga
    50941 agcgatcatt aaccctaact tgcgcgactg taatttcccg tcgaaatcac tggcaggcgt
    51001 gggtgtggcg ttttatctga tgctggcgct gcgcaccttt ttgcgcgatc agggctggtt
    51061 tgatgagcgc ggcatcgcaa ttcctaacct ggcagaactg ctggatctgg tagcgctggg
    51121 gacagtagcg gacgtcgtgc cgctggacgc taataatcgc attctgacct ggcaggggat
    51181 gagtcgcatc cgtgccggaa agtgccgtcc agggattaaa gcgctgctgg aagtggcaaa
    51241 tcgcgatgca caaaaactag ccgccagcga tttaggtttt gcgctgggac cgcgtctcaa
    51301 tgctgccggg cgactggatg atatgtccgt tggtgtggcg ctgttgctgt gcgacaacat
    51361 cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc gaaaagagat
    51421 cgaacaagga atgcaggttg aagccctgac cctgtgcgag aaactggagc gcagccgtga
    51481 cacgctaccc ggcgggctgg caatgtatca ccccgaatgg catcagggcg ttgtcggtat
    51541 tctggcttcg cgtatcaaag agcgttttca ccgtccggtt atcgcctttg cgccagcagg
    51601 tgacggtacg ctgaaaggtt ccggtcgctc cattcagggg ctgcatatgc gtgatgcgct
    51661 ggagcgatta gacacactct accccggcat gatgctcaag tttggcggtc atgcgatggc
    51721 ggcgggtttg tcgctggaag aggataaatt cgaactcttt caacaacggt ttggcgaact
    51781 ggttactgag tggctggccc cttcgctatt gcaaggcgaa gtggtgtcag acggtccgtt
    51841 aagcccggcc gaaatgacca tggaagtggc gcagctgctg cgcgatgctg gcccgtgggg
    51901 gcagatgttc ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac agcggctggt
    51961 gggcgaacgt catttgaagg tgatggtcga accggtcggc ggcggtccac tgctggatgg
    52021 tattgctttt aatgtcgata ccgctctctg gccggataac ggcgtgcgcg aagtgcaact
    52081 ggcttataag ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa ttatcatcga
    52141 caatatctgg ccaatttagc gtcatcttct ctataaaaaa gagcgtggat tgggtacaat
    52201 cccgctctta tcaccgcatt ttgactagct caataaaaga aatcagacca tgtttgaaat
    52261 taatccggta aataatcgca ttcaggacct cacggaacgc tccgacgttc ttagggggta
    52321 tctttgacta cgacgccaag aaagagcgtc tggaagaagt aaacgccgag ctggaacagc
    52381 cggatgtctg gaacgaaccc gaacgcgcac aggcgctggg taaagagcgt tcctccctcg
    52441 aagccgttgt cgacaccctc gaccaaatga aacaggggct ggaagatgtt tctggtctgc
    52501 tggaactggc tgtagaagct gacgacgaag aaacctttaa cgaagccgtt gctgaactcg
    52561 acgccctgga agaaaaactg gcgcagcttg agttccgccg tatgttctct ggcgaatatg
    52621 acagcgccga ctgttacctc gatattcagg cggggtctgg cggtacggaa gcacaggact
    52681 gggcgagcat gcttgagcgt atgtatctgc gctgggcaga atcgcgtggt ttcaaaactg
    52741 aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa atccgtgacg atcaaaatct
    52801 ccggcgatta cgcttacggc tggctgcgta cagaaactgg cgttcaccgc ctggtgcgta
    52861 aaagcccgtt tgactccggc ggtcgtcgcc acacgtcgtt cagctctgcg tttgtttatc
    52921 cggaagttga tgatgatatt gatatcgaaa tcaacccggc ggatctgcgc attgacgttt
    52981 atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg gtgcgtatta
    53041 cccacatccc gaccgggatc gtgacccagt gccagaacga ccgttcccag cacaagaaca
    53101 aagatcaggc catgaagcag atgaaagcga agctttatga actggagatg cagaagaaaa
    53161 atgccgagaa acaggcgatg gaagataaca aatctgacat cggctggggc agccagattc
    53221 gttcttatgt ccttgatgac tcccgcatta aagatctgcg caccggggta gaaacccgca
    53281 acacgcaggc cgtgctggac ggcagcctgg atcaatttat cgaagcaagt ttgaaagcag
    53341 ggttatgagg aaccaacatg tctgaacaac acgcacaggg cgctgacgcg gtagtcgatc
    53401 ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa cctgcgtgag cagggaattg
    53461 ccttcccgaa cgatttccgt cgcgatcata cctctgacca attgcacgca gaattcgacg
    53521 gtaaagagaa cgaagaactg gaagcgctga acatcgaagt cgccgttgct ggccgcatga
    53581 tgacccgccg tattatgggt aaagcgtctt tcattaccct gcaggatgtg ggtggccgca
    53641 ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag cagttcaaaa
    53701 aatgggacct cggcgacatc ctcggcgcga aaggtaaact gttcaaaacc aaaaccggcg
    53761 aactgtctat ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt ccgctgccgg
    53821 ataaattcca cggcttgcag gatcaggaag cgcgctatcg tcagcgttat ctggatctca
    53881 tctctaacga tgaatcccgt aacaccttta aagtacgctc gcagatcctc tctggtattc
    53941 gccagttcat ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg caggtgatcc
    54001 caggcggtgc cgctgcgcgt ccgtttatca cccaccataa cgcgctggat ctcgacatgt
    54061 acctgcgtat cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc ttcgagcgtg
    54121 tattcgaaat caaccgtaac ttccgtaacg aaggtatttc cgtacgtcat aacccagagt
    54181 tcaccatgat ggaactctac atggcttacg cagattacaa agatctgatc gagctgaccg
    54241 aatcgctgtt ccgtactctg gcacaggata ttctcggtaa gacggaagtg acctacggcg
    54301 acgtgacgct ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa gcgatcaaga
    54361 aatatcgccc ggaaaccgac atggcggatc tggataactt cgactctgcg aaagcgattg
    54421 ctgaatctat cggtatccac gttgagaaga gctggggtct gggccgtatc gttaccgaga
    54481 tcttcgaaga agtggcagaa gcacatctga ttcagccgac cttcattact gaatatccgg
    54541 cagaagtttc tccgctggcg cgtcgtaacg acattaaccc ggaaatcaca gaccgctttg
    54601 agttcttcat cggtggtcgt gaaatcggta acggctttag cgagctgaat gacgcggaag
    54661 atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga cgcaggtgac gacgaagcga
    54721 tgttctacga tgaagattac gttaccgcac tggaacatgg cttaccgccg acagcaggtc
    54781 tggggattgg catcgaccgt atggtaatgc tgttcaccaa cagccatacc atccgcgacg
    54841 ttattctgtt cccggcgatg cgtccggtga aataagcatt acgttatgct cacaaccccg
    54901 gcaaatgtcg gggtttttta tttaagctgg gtaaatgcag ataatcgtat tctggcttcg
    54961 cgatttgtcg cctgcatcac catccacgga ctgaacgccc acggcgtggc atcaataccg
    55021 cgtaatacat cagctaaatc acaccattga taatccatca cttcatcatc gttgatctgt
    55081 aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt cattttccac aatgccactc
    55141 ggatcggtgg cgcggtagcg aaagtcagga tagatagatt caggaggcgt aatttccacg
    55201 ccaagctcat aacggcaacg gcggatcacc gcgtcttcgc tgctttctcc cagttgtggg
    55261 tgcccacaaa ccgagttagt ccacacgcca ggccacgctt ttttgctcag tgcgcggcgg
    55321 gtaactaata attgcccctt ggcattaaac agccaactgg agaacgcgag atgtaagagg
    55381 gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg tgggaactcc ctgtgcattc
    55441 aataaaatga cgtgttccgt ttgcataatt tctcacatgt aattctgatc actcatcatt
    55501 atagataatt gaagagtgaa atgattaacc aagagagcgt ttatcgacaa ccgcccgttt
    55561 tgagcggatg ccgacaggta aaaaccattt aaaaaagtag catcatatat ttgaagcgac
    55621 tttcaatggc cattgcaaca cagtcagaaa tgacaggaat agagagagta aaaagcaaca
    55681 acagactgaa tatagcgcgc attaaaaaat tcatattatt ctccatgctc atttttcttc
    55741 agcttgcgca tcagaagcca catacgtact gtcctgactt ttaaatcttt atcagagacg
    55801 gattccttca ccaggtcagc atgatgctga tagccattaa agaagacgtt cagcacaacg
    55861 gcgctaaggg ttgcgagcat aatgccgcta tgtagcagcg gttgcagtac ggccggtaac
    55921 ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact gatcgccaca
    55981 atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg aatccctgtc
    56041 gccagtacca tgccgaacat cactagacca gcgccgccca gcacaaattg cggaatggag
    56101 gctaccagca ccgccatttt tggcaccatg ccaaacagga ttaatataat tcccgaagaa
    56161 atacataccc agcggctatg aacgcgcatc acgctaacca ggccaacgtt ttgagaaaat
    56221 gacgtgtggg ggaagctgtt aaacgtgccg cctatcattg tccctacgcc atcgacacgc
    56281 agcccgcgaa taatatcgtg tgaagagagt ttacgaccga ctatttcacc cagtgccagg
    56341 aacatcccca tcgactcgat aaacacgatg attaacacgg cagtcatggt cagaatggaa
    56401 acgggatcga aaatcggcat accaaacgac atcggggtga caatcgcaaa ccacgaggca
    56461 tcatgtagcc cggataaatt gacttcattc atcatccacg aaagtaaaaa gccaaataca
    56521 atccccagta atacggcgac gttggacata aatcctttcg catagcgagt aatgagcaag
    56581 ataaaaatta agacggcaaa ggagatacct aaataaacgg gattaccata ttgcggattc
    56641 cctttacctc cagcggccca gtcaataccc acctgaatga tgctaagccc aatagaagta
    56701 ataaccacac cggtaaccag tggcgggaat aaaggcatca agcgaccaat aagtggcgct
    56761 aataatgtgg tgataaaacc cgcggcgata gtggcaccaa atatccccag caggccgata
    56821 tccgggttca tcccaatggc tatcattggt gttacagcag caaaggtcac cgacataatc
    56881 accggcaggc ggatccccat aaagcggccg ataccgatac attgcaataa tgtgacgatc
    56941 ccgcagcaaa agagatccga gctaatgagc atcgcaatag catcttttga gaggcccagt
    57001 cggtcaccaa tcataagagg aacagcgact gcacctgcgt acatgacaag gacgtgttgc
    57061 agaccgagta tgatcagctt tcctggtgat aatatgcggt caacctcatc agttgggcgg
    57121 tcttgcccag aagatgaggg aagttgggaa tctatggcgc tcatcagtgt ttctcctttc
    57181 gcccgccaga ataaaaagca accgtttacg ttaatgaaac tgtctaaaca catgctcgtg
    57241 gagtgccagt ttttgcgagg aaggggagtt agagacagga attgcggagg caatcacata
    57301 atcgtgctta aaccacgaaa atgacaagag aacctgacaa tggaattatc tgtaattttc
    57361 tcatattgat atttcattaa tcattctggc atgtcgcact ctcgcattta atcgttttta
    57421 tctggatagc gctcttttga tcggcaatgt tgaaataatg aaagcggcat agattgaggg
    57481 gcaataattt gaactcagtc gcaaaattat aattaaaacg gtcatgctac ctaatatatg
    57541 cccgtttatt gcatcgcgaa atttgagaag acataactca ttaaatgaaa gggatgcaaa
    57601 tgaataagtt tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct tgtgaaattg
    57661 cctgtgcggt ggcacacaat caagaaaact ggccgctgag tcacagtgac tttcgaccgc
    57721 gtatccacgt tgtagggaaa ggccaggctg cgaatccggt ggcctgccat cactgcaaca
    57781 atgccccttg tgttacggct tgtccggtta atgctctgac tttccagtcc gatagcgtac
    57841 aactggacga gcaaaaatgt attggctgta aaagatgcgc aatcgcttgc ccctttggcg
    57901 tcgttgagat ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag cgcagttccg
    57961 gcacgcaagc ctgtattgaa gtctgcccaa cacaggcgtt acgactgatg gacgataaag
    58021 ggttacagcg gataaaggtg gcccgccagc gcaaaacggc agcaggaaaa gcgtcatcag
    58081 acgctcagcc atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa ggcgcagata
    58141 aaatttcagc gagtgaacgg aaaacccact ttggcgaaat ctattgcggg ctggatccac
    58201 tgcaagcgac ttatgagagt gaccgctgtg tttattgtgc cgaaaaagct aactgcaact
    58261 ggcattgccc gctgcataac gctattccgg attacatccg tctggtacag gaaggaaaga
    58321 ttattgaagc ggcagaactt tgccaccaga ccagttcctt accagaaatc tgcggcaggg
    58381 tatgcccaca ggaccgtctt tgtgaaggcg catgtacttt gaaagatcac tcaggcgcag
    58441 tctctatcgg taatctggaa cgctacatca ccgataccgc gctggcgatg ggctggcgtc
    58501 ccgatgtcag caaagttgtt ccccgtatcg aaaaagtggc ggtgattggc gctgggccag
    58561 cagggttagg gtgtgctgat attctggcgc gcgcgggagt tcaggttgat gtctttgagc
    58621 gccatccaga aattggcggt atgctgactt ttggcattcc tcctttcaaa ctcgataaaa
    58681 cggtattaag ccagcggcga gagatattca ccgcaatggg aatcgacttt catcttaact
    58741 gtgaaattgg ccgcgatatt acctttagcg atttaacttc tgaatatgat gcagttttca
    58801 tcggcgtggg gacttacggg atgatgcgag cagatctgcc gcatgaagat gcgcccggtg
    58861 tcattcaggc tctaccgttc ctgactgccc atacccgcca gctcatggga ttgccggagt
    58921 ctgaagagta tccgctgacg gacgtggaag gtaagcgagt cgtggtattg ggcggtggcg
    58981 atacgacaat ggattgtttg cggacttcca ttcgcctcaa tgctgccagc gtgacctgcg
    59041 cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtc aatgcgcgcg
    59101 aggaaggtgt cgagtttcag ttcaatgttc agccgcaata tatcgcttgt gacgaagatg
    59161 ggcgtttaac tgcagtgggc ctgattcgta ccgccatggg ggagcagggg ccggatggtc
    59221 gccgtcgtcc tcgtccggta gcgggttcag agtttgaatt gcccgccgat gttctgatta
    59281 tggcctttgg tttccaggcg catgccatgc cgtggttgca gggcagcgga attaaactcg
    59341 ataaatgggg cctgattcaa accggtgacg tcgggtattt acctacccag acgcatctga
    59401 aaaaagtctt tgctggtggt gctgcagttc atggcgcgga tctggttgtc actgccatgg
    59461 ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt tgatacgaag gcatcgtgat
    59521 gaaatcgtta attatcgtta atccggctga ctgtattggc tgccgtactt gtgaagtagc
    59581 ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc gatgtctttt taccccggct
    59641 gaaggtacag cgactggaca tcatcagtgc gccggtgatg tgccatcagt gtgaaaacgc
    59701 tccttgtgtt ggcgcttgcc ccgtgcaggc gctgacgatg ggcgagcagg tggtgcaggc
    59761 caattctgcc cgttgtattg gctgtcagag ctgcgtcagt gcgtgtccgt ttgggatgat
    59821 caccattcag tcgttgccgg gggatacccg gcaacaaatc gtgaaatgcg atctctgtga
    59881 acagcgggaa gaggggccag cctgcgttga atcttgcccg acacaggcgc tgcagttgct
    59941 tactgaaaga gaactcaggt gactccgcca gcagcgtatt gttgccagcg gtgagaatcc
    60001 tctctgaaag aaatctccgc gctgaaaggc gcggagaatc tattagattg cccaaccacc
    60061 cgcgtaaaat gcgaccagtg cggcagtaat gatcaccgta ccaatattca gcttttgcca
    60121 ttcgcgtgca aagacgcggc ctacgaccag tgtcacaaag cccagcataa taccggtaac
    60181 gatattacaa gtcagaacga tgaacacggc acacaccagg ccagccatag cgtcaataaa
    60241 atcattgaaa tccagcttcg agacgttact taacatcagc aaacccacgt acatcagagc
    60301 gggggcagtg gcgtaaccag ggatcaaaaa tgacagcggt gataaaaaca gaattaacag
    60361 gaataacgcc cccactacgg ttgcggtcaa ccctgtttta cccccggcgg cggttcctgc
    60421 cgctgattcg atataaaccg ctgcgggcgc tgcgcccacc aggccggaga atattgaact
    60481 tactgagtca ctggtcaggg ctttgccgcc gttgatgatc tggttgtctt tatccaacaa
    60541 attcgcctga ccggcgacag cacggatggt gccagtagcg tcgaacactg cggtcatcac
    60601 caatgccagt acactcggaa gtacagttgg ctggagtgcg cccataatat cgaggctgaa
    60661 aatcagagac ttaccatctt cgccagtcag gcttggcatc gccaccagac cgtggtattt
    60721 caccgctggg tcaaagatta agccgatgat cgaaattgca ataatcacca acaagatccc
    60781 gccgggtacg cgacacttct ccagaccgaa gatgaccgcc agccccagta agctcatcat
    60841 caccgggaag gaggtaaacg caccgagcgc cactggcaag ccttcaatcg ggtttttgat
    60901 aaccataccc acaccgttag cagcaatcag cagcagaaac agcccgatac cgatacctgt
    60961 accgtgagcg atacccatcg gcaaattacg taagatccag gtacgcacac cggttacgga
    61021 aatggcggtg aagatgacgc ccatcagaaa taccgcgccc agtgcgacag gaacgctaat
    61081 ttgttgcccg agtaccagac tgaatgcggt aaacgccgtc aaggaaatcg cgcaaccaat
    61141 cgccattggc aaattagccc ataatcccat cagcaacgag ccgaagcccg cgaccagaca
    61201 ggtggcaaca aacacagctg cgggaggaaa acctgctttg cccagcattc ccggaacgac
    61261 gataacggaa taaaccatgg ccagaaaggt cgttaagcca gccagtactt cctgacgaac
    61321 ggtactgcca cgagcggtaa ttttaaaata attatccagc gcgccctgtg gctttggtgc
    61381 gtccggtgtt tgtaggatgt ctccagacat aatgatgtcc tcacgaggat gttaaagttt
    61441 tattattagt tgcgttcgta ccccagacga ccatcaacgt aggtgcggta gatcgaacgg
    61501 tcatcgccca acgtcatcat cacgaacaat ttgtcgacta aagaaacaga gttgtcatag
    61561 cgcagctgct gtagcggagt ggcggtgggt tccatcacca cgaaatcagc ctctttgcta
    61621 ggtaaaaagt tgccaatcaa atcgtcaagg cccagagatt tcgctccgcc gagcgtggcc
    61681 aggtaaaacg cttcatatgc cgagaggcga tagccttgta attgcaatac tttgtaggct
    61741 tcgttcagcg tttgcagcat gttgaaagtg gttccggcac cgatatccgt tcccatgccc
    61801 actttaactt tcttctgcca tgcttttttc aagttgaata agccgctgcc gaggtaaagg
    61861 ttggaggtcg gacagaaagc aatgctggat ttggtttcgc tgagacgatc ccactctttt
    61921 tcttcgagat ggacgcagtg agcaaagaca cagtttttac cggtcagacc gtactgatgg
    61981 taaacatcca gataaccatc atggtcagga taaagcgatt tcacccaggc aatttcatct
    62041 ttgttttcac agagatgggt atgtacccac gtatccggat attcttcttt caggcgttgc
    62101 gccatcgcca tctgttcagg agatgaggta ggggcgaagc gtggcgtaat cgcatacaac
    62161 agacgaccat ttttgtgcca gcgttcgatc agttctttgc tttggtgata gctgctttcg
    62221 gcagtgtcga gcagataatc cggtgcgttg cggtccatca tcaccttacc ggcaatcata
    62281 cgcatattga tatgactggc ggcttcaaac agcgcatcaa cagattgcgg atgaacagtg
    62341 ccaaacacca gcgccgtggt ggttccgtta cgtaaaagct gcttgatgaa gaacgccgac
    62401 atttcgcggg cgtactctaa atcctcataa cgacgttcag tagggaaggt gtgtttattc
    62461 aaccactcca gcaattgctc accataggcc cccaccattt cactttgcgg ataatggata
    62521 tgtgtatcga caaagcccgg tactatcagt ttgccgcgat agtcgcgcac gcgaatggtg
    62581 tcaggaattt gatgctttcc gttttcccat tcgccaaacc attccacttt tccctgttta
    62641 atgagtaata aaccatcctc aataaaccgc agcgcagagg caatctcttc cgggttatcg
    62701 accgtacggg tgacatcaat aaaactgcct cgtaccgctt ttaacgtgtg ttctcctgac
    62761 atcattgact cctttactgt taatccatct cttccgctga ggtaatacca ggcagaacgt
    62821 tttcctgtcg gtagccacca gggaggataa tatttaataa aatcgcagtt aacccgccag
    62881 cacaaatagg gttttcaact aatacataaa tagaggctgg caatatttta aaaatttcgg
    62941 gatcgtagga gacgccaagc cctaaaccta aagaagtggc gacaataagt gtttcacggc
    63001 gctttaaacc gttggtgatg atgatgcgaa tcccagcgat ggcaatcatg gaaaacatca
    63061 acgtcattgc gcctcccaga actgccgagg gaatggtcgt gaagaagccg ccaatcatcg
    63121 gaaacaaacc gagtataacc agcattacca cgatggttcg cccgacataa cgtgaagcga
    63181 cgccagtcat ctgaataacc ccattatttt gcgcaaacgt ggttaatggt aatgaaccga
    63241 cagcggaggc gataacagaa accagaccat ctgccagcac gccgcctttc agtcgggact
    63301 gatactcttc cccctgaatg gggcggcggg aaaccattgc cgtggcggtg atatcgccga
    63361 ctgcttccag cacgctaagc agataaatcg tgccaaccac caggaactga tggaagctaa
    63421 aactaaagcc gtatttgaac ggatgcggga tggtgattaa cggcaaattg cgcatactgc
    63481 tgaaatccac catgcctagg cataacgatg cgatatagcc gacacatagc ccaatggcga
    63541 tccctcccat gcgtagcaac ggactgcgac agcagttaaa gccgatcacc acaattaaaa
    63601 ccaataaacc aacgccgaga tgtgcgtaat tgccgaacgt accgctgctt ttggctgcaa
    63661 atcctccacc aaaatcgata atgccgactt taatcaggct taagccgatc atcagtacca
    63721 caataccgct gacggtaggc gtaataaccc gacgtaaata gggcaagata aatgaagatc
    63781 cgacaaccag aaatgcgcca acgaaggaga cgccgagaag cgacgacatg attaactctt
    63841 cgtgaaaacc gtcgcttttc atgctgctgc ccagcgcaat catgaccgta acaaatgaaa
    63901 aattgactga ctgaattgag agtaggccag aaccgacgat gccgtagcgg tttacttgta
    63961 actaggtacc aataccagag gcgatcatcg ccatagaaac aagataggca gttgtttcag
    64021 cggaaagctg taaggccgcc cccacgatta acgcgggggt taccatcgga acgaaaattg
    64081 ccaacagatg ggtaatggca ccaacgagag cctgatgaaa gggagggcga tcctccagtt
    64141 caaatataag gtcagaacct gcatggttta tatcagacat cccttcactc cttttaaagt
    64201 aaatttgatc atctgaatta gtaaaataaa ttcccacaaa tttggggcgc aggacgcctt
    64261 gagaacggct tatctcattt tcgcagtcac tatttagtca tcgttcaaac
//
LOCUS       DADKXM010000008        62419 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.008, whole genome shotgun sequence.
ACCESSION   DADKXM010000008 DADKXM010000000
VERSION     DADKXM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.008"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            214..717
                     /locus_tag="JQR05_000495"
     CDS             214..717
                     /locus_tag="JQR05_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY5322737.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(784..2241)
                     /gene="nuoN"
                     /locus_tag="JQR05_000496"
     CDS             complement(784..2241)
                     /gene="nuoN"
                     /locus_tag="JQR05_000496"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY5322738.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYALVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2248..3777)
                     /gene="nuoM"
                     /locus_tag="JQR05_000497"
     CDS             complement(2248..3777)
                     /gene="nuoM"
                     /locus_tag="JQR05_000497"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY5322739.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3941..5782)
                     /gene="nuoL"
                     /locus_tag="JQR05_000498"
     CDS             complement(3941..5782)
                     /gene="nuoL"
                     /locus_tag="JQR05_000498"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY5322740.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5779..6081)
                     /gene="nuoK"
                     /locus_tag="JQR05_000499"
     CDS             complement(5779..6081)
                     /gene="nuoK"
                     /locus_tag="JQR05_000499"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY5322741.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6078..6632)
                     /gene="nuoJ"
                     /locus_tag="JQR05_000500"
     CDS             complement(6078..6632)
                     /gene="nuoJ"
                     /locus_tag="JQR05_000500"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY5322742.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6644..7186)
                     /gene="nuoI"
                     /locus_tag="JQR05_000501"
     CDS             complement(6644..7186)
                     /gene="nuoI"
                     /locus_tag="JQR05_000501"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY5322743.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7201..8178)
                     /gene="nuoH"
                     /locus_tag="JQR05_000502"
     CDS             complement(7201..8178)
                     /gene="nuoH"
                     /locus_tag="JQR05_000502"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY5322744.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8175..10901)
                     /gene="nuoG"
                     /locus_tag="JQR05_000503"
     CDS             complement(8175..10901)
                     /gene="nuoG"
                     /locus_tag="JQR05_000503"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY5322745.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10954..12291)
                     /gene="nuoF"
                     /locus_tag="JQR05_000504"
     CDS             complement(10954..12291)
                     /gene="nuoF"
                     /locus_tag="JQR05_000504"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY5322746.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12288..12788)
                     /gene="nuoE"
                     /locus_tag="JQR05_000505"
     CDS             complement(12288..12788)
                     /gene="nuoE"
                     /locus_tag="JQR05_000505"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY5322747.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12791..14593)
                     /gene="nuoC"
                     /locus_tag="JQR05_000506"
     CDS             complement(12791..14593)
                     /gene="nuoC"
                     /locus_tag="JQR05_000506"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY5322748.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPTAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14687..15349)
                     /locus_tag="JQR05_000507"
     CDS             complement(14687..15349)
                     /locus_tag="JQR05_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY5322749.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15365..15808)
                     /gene="nuoA"
                     /locus_tag="JQR05_000508"
     CDS             complement(15365..15808)
                     /gene="nuoA"
                     /locus_tag="JQR05_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY5322750.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16439..17377)
                     /gene="lrhA"
                     /locus_tag="JQR05_000509"
     CDS             complement(16439..17377)
                     /gene="lrhA"
                     /locus_tag="JQR05_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY5322751.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKSGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18297..19514
                     /locus_tag="JQR05_000510"
     CDS             18297..19514
                     /locus_tag="JQR05_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY5322752.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19598..20197
                     /gene="yfbR"
                     /locus_tag="JQR05_000511"
     CDS             19598..20197
                     /gene="yfbR"
                     /locus_tag="JQR05_000511"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY5322753.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20256..22088)
                     /locus_tag="JQR05_000512"
     CDS             complement(20256..22088)
                     /locus_tag="JQR05_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY5322754.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASTAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22175..22825)
                     /gene="hxpA"
                     /locus_tag="JQR05_000513"
     CDS             complement(22175..22825)
                     /gene="hxpA"
                     /locus_tag="JQR05_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY5322755.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22836..23330)
                     /locus_tag="JQR05_000514"
     CDS             complement(22836..23330)
                     /locus_tag="JQR05_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY5322756.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23413..23868)
                     /locus_tag="JQR05_000515"
     CDS             complement(23413..23868)
                     /locus_tag="JQR05_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY5322757.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24206..25408
                     /gene="ackA"
                     /locus_tag="JQR05_000516"
     CDS             24206..25408
                     /gene="ackA"
                     /locus_tag="JQR05_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY5322758.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDYERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25483..27627
                     /gene="pta"
                     /locus_tag="JQR05_000517"
     CDS             25483..27627
                     /gene="pta"
                     /locus_tag="JQR05_000517"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY5322759.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27817..29337
                     /gene="yfcC"
                     /locus_tag="JQR05_000518"
     CDS             27817..29337
                     /gene="yfcC"
                     /locus_tag="JQR05_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY5322760.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMDSSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29370..29912)
                     /gene="yfcD"
                     /locus_tag="JQR05_000519"
     CDS             complement(29370..29912)
                     /gene="yfcD"
                     /locus_tag="JQR05_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY5322761.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29970..30521)
                     /gene="yfcE"
                     /locus_tag="JQR05_000520"
     CDS             complement(29970..30521)
                     /gene="yfcE"
                     /locus_tag="JQR05_000520"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY5322762.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30577..31221)
                     /gene="yfcF"
                     /locus_tag="JQR05_000521"
     CDS             complement(30577..31221)
                     /gene="yfcF"
                     /locus_tag="JQR05_000521"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY5322763.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31357..32004
                     /gene="yfcG"
                     /locus_tag="JQR05_000522"
     CDS             31357..32004
                     /gene="yfcG"
                     /locus_tag="JQR05_000522"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY5322764.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKWLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32061..32423
                     /gene="folX"
                     /locus_tag="JQR05_000523"
     CDS             32061..32423
                     /gene="folX"
                     /locus_tag="JQR05_000523"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY5322765.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32444..33337
                     /locus_tag="JQR05_000524"
     CDS             32444..33337
                     /locus_tag="JQR05_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY5322766.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33385..34275)
                     /locus_tag="JQR05_000525"
     CDS             complement(33385..34275)
                     /locus_tag="JQR05_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY5322767.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34472..35245)
                     /gene="hisP"
                     /locus_tag="JQR05_000526"
     CDS             complement(34472..35245)
                     /gene="hisP"
                     /locus_tag="JQR05_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY5322768.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35253..35969)
                     /locus_tag="JQR05_000527"
     CDS             complement(35253..35969)
                     /locus_tag="JQR05_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5322769.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILILHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35966..36652)
                     /locus_tag="JQR05_000528"
     CDS             complement(35966..36652)
                     /locus_tag="JQR05_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5322770.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36742..37524)
                     /gene="hisJ"
                     /locus_tag="JQR05_000529"
     CDS             complement(36742..37524)
                     /gene="hisJ"
                     /locus_tag="JQR05_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY5322771.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37745..38527)
                     /gene="argT"
                     /locus_tag="JQR05_000530"
                     /pseudo
     CDS             complement(37745..38527)
                     /gene="argT"
                     /locus_tag="JQR05_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(38793..39362)
                     /locus_tag="JQR05_000531"
     CDS             complement(38793..39362)
                     /locus_tag="JQR05_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY5322772.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39457..40974)
                     /gene="purF"
                     /locus_tag="JQR05_000532"
     CDS             complement(39457..40974)
                     /gene="purF"
                     /locus_tag="JQR05_000532"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY5322773.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGVIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41011..41499)
                     /gene="cvpA"
                     /locus_tag="JQR05_000533"
     CDS             complement(41011..41499)
                     /gene="cvpA"
                     /locus_tag="JQR05_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY5322774.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41758..42420)
                     /gene="dedD"
                     /locus_tag="JQR05_000534"
     CDS             complement(41758..42420)
                     /gene="dedD"
                     /locus_tag="JQR05_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY5322775.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42410..43678)
                     /gene="folC"
                     /locus_tag="JQR05_000535"
     CDS             complement(42410..43678)
                     /gene="folC"
                     /locus_tag="JQR05_000535"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY5322776.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELLE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43748..44662)
                     /gene="accD"
                     /locus_tag="JQR05_000536"
     CDS             complement(43748..44662)
                     /gene="accD"
                     /locus_tag="JQR05_000536"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY5322777.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44818..45477)
                     /locus_tag="JQR05_000537"
     CDS             complement(44818..45477)
                     /locus_tag="JQR05_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5322778.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45560..46372)
                     /gene="truA"
                     /locus_tag="JQR05_000538"
     CDS             complement(45560..46372)
                     /gene="truA"
                     /locus_tag="JQR05_000538"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY5322779.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46372..47385)
                     /locus_tag="JQR05_000539"
     CDS             complement(46372..47385)
                     /locus_tag="JQR05_000539"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY5322780.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERCIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47451..48587)
                     /gene="pdxB"
                     /locus_tag="JQR05_000540"
     CDS             complement(47451..48587)
                     /gene="pdxB"
                     /locus_tag="JQR05_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY5322781.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQVRLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48686..49681
                     /locus_tag="JQR05_000541"
     CDS             48686..49681
                     /locus_tag="JQR05_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY5322782.1"
                     /translation="MIQPISGPPPRQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49678..50854)
                     /locus_tag="JQR05_000542"
                     /pseudo
     CDS             complement(49678..50854)
                     /locus_tag="JQR05_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(51129..52349)
                     /gene="fabB"
                     /locus_tag="JQR05_000543"
     CDS             complement(51129..52349)
                     /gene="fabB"
                     /locus_tag="JQR05_000543"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY5322783.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52508..54514
                     /gene="mnmC"
                     /locus_tag="JQR05_000544"
     CDS             52508..54514
                     /gene="mnmC"
                     /locus_tag="JQR05_000544"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY5322784.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54635..54913)
                     /locus_tag="JQR05_000545"
     CDS             complement(54635..54913)
                     /locus_tag="JQR05_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY5322785.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(54947..55495)
                     /locus_tag="JQR05_000546"
     CDS             complement(54947..55495)
                     /locus_tag="JQR05_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY5322786.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55495..56304)
                     /locus_tag="JQR05_000547"
     CDS             complement(55495..56304)
                     /locus_tag="JQR05_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY5322787.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEYRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56304..57128)
                     /gene="mepA"
                     /locus_tag="JQR05_000548"
     CDS             complement(56304..57128)
                     /gene="mepA"
                     /locus_tag="JQR05_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY5322788.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57132..58217)
                     /gene="aroC"
                     /locus_tag="JQR05_000549"
     CDS             complement(57132..58217)
                     /gene="aroC"
                     /locus_tag="JQR05_000549"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY5322789.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58252..59184)
                     /gene="prmB"
                     /locus_tag="JQR05_000550"
     CDS             complement(58252..59184)
                     /gene="prmB"
                     /locus_tag="JQR05_000550"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY5322790.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNQWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTCRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59350..59901
                     /gene="smrB"
                     /locus_tag="JQR05_000551"
     CDS             59350..59901
                     /gene="smrB"
                     /locus_tag="JQR05_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY5322791.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60005..60895)
                     /locus_tag="JQR05_000552"
     CDS             complement(60005..60895)
                     /locus_tag="JQR05_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY5322792.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSVAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(60882..61406)
                     /locus_tag="JQR05_000553"
     CDS             complement(60882..61406)
                     /locus_tag="JQR05_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5322793.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPVNNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61403..61873)
                     /locus_tag="JQR05_000554"
     CDS             complement(61403..61873)
                     /locus_tag="JQR05_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5322794.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(61870..62418)
                     /locus_tag="JQR05_000555"
     CDS             complement(61870..62418)
                     /locus_tag="JQR05_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5322795.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15556 a        17011 c        15621 g        14231 t
ORIGIN      
        1 tatcgtaata aacaatacca cttgcccggt gataccgcag acagattgaa ttatcagaaa
       61 atggctcagg tggtagatgg tgttataacc ttgttataca acagtaaata ataaaaattt
      121 ctcgaggcgg gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga
      181 tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa tgattggcta ttttgcagaa
      241 attgactcag aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat
      301 atacatgaca cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta
      361 cactttgggc tgacaggcac ctctgctttt gatcccgcga agaatgatcc gctgagtcgt
      421 gccgtgcttg atgaacacag cctcgaagat ggcatagatg gcttcctggg attgacctgg
      481 aatcaggaat tagccgcgac aatcgaccga ctggaatcac tggatcgaag tgagctgcgt
      541 aaacagtttt ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca
      601 gaagagttag aggggcaact ctttgcctcg atcatgcttg atatggaaaa gctgattagc
      661 gcgtatcgac gtatgctgcg acaagggaat catgcgctga cggttattgt tgggtgatgg
      721 tgtcagagcg ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt acaaactaac
      781 agtttacatc agcggcattg ctaaccgcac aatactaatc agcggttgtg gccatacacc
      841 cagcaccagt accaacagtg cagaaatcag caccacgata ccgcccgcgc tgtactgcca
      901 gtttgacggt gcatcgcgac ccggttgttc cggtgcgtgc aggtacaggc tcaccgccac
      961 gcgcaggtag tagtagaggc cgattgccga accgacaacc acggcaccca ccagccacca
     1021 caagtgtgcc tggacaccga ccgccagcac gtagaactta ccgataaagc ccagcgtcat
     1081 cgggataccg gccagagaca gcatcatcac cgtcatcact gccgcgagga tcggacgatg
     1141 ccagaacaaa ccgcggtaag agaacaggga atcagcatcc gggccacgat acgggctgga
     1201 catcaggctg accacgccga acgcgccgag gctgctgaac agataaccgg ccaggtaaac
     1261 ccctaccgct tccatcgaca tctcgccggt ttgcagcgca atcagcgcca ccagcagata
     1321 gccgaggtga gagatagatg agtaaccgag cagacgtttg atgttggtct ggctcaacgc
     1381 catcaggtta ccgaagatga tggaggcaaa ggcgataatc gccagtacta cgcgaatcgc
     1441 ttcgctgtca cccaccagtg cgtagaggaa cagacgcatc accacaccga agatagcgat
     1501 tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc gcgccctggt atacgtctgg
     1561 cgtccacagg tggaacggca ccagagagag tttgaagccg aggccaacaa tcatcatgcc
     1621 gaaacctgcc agcaacagcg gctcgttgag cataccgtcg ccgaggtttt tacccagcgc
     1681 gacaaacgac aggtcgccag actgcgcata caccagcgcc ataccgaaca gcaggaaaga
     1741 agacgctgcg gcagaaagga tggtgtattt gatactggct tccagcgaac gtttctggcg
     1801 gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc agttcaatac cgaggaacag
     1861 agacgccaga tggtttgcat tcgccagcag gatcccgccc agcgcggcaa ttaacaccaa
     1921 caggtagaac tcatccttgt tgtcgttata gccttcaagc cacgggtagg cgaaagtaca
     1981 ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac catcaacgcg
     2041 catcagcggc gtaacgtcca tagcgcccgc ctggccaaca aaccagagcg aaaccagcgc
     2101 cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat
     2161 ggagagcatc acaaccacca ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag
     2221 gttttgtgga gttattgtca tggcgattta cggccttgta gtagtaacgg aattaacaaa
     2281 ccactgctgg atattgccaa tcgcggagtg cgaggtatcc agaatcggct gcggatagaa
     2341 gcccagcagt accagcagca ccaccagcaa caggatcata aacagctcac gcagcgacat
     2401 ccctggcagt tcctggctgg caatctggct tttcgcttta ccgaagtaag cgcgatgcaa
     2461 catcgccagc gaataaacag atgcaaagac cagcccaaag gtagagataa cggtaattac
     2521 cgggacaacc tggaagctgc cgaacagaat cataaattcg ccgacgaagt taccggtgcc
     2581 aggcatccca agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc atttcatctt
     2641 gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc
     2701 gcagagaata aacagacccg ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc
     2761 ctggtaggcc aactggctgc cggtgtagat tgcaatcagc acgaagccca tgtgggaaac
     2821 cgaggtgtag gcgatcagac gtttgatatc ggtctgagcg aaggtcatcc acgcaccgta
     2881 gaagatgccg ataacaccca gccacatagc gattggcgcg aactctgccg acgcgttcgg
     2941 gaacagcggc agggagaaac gcaacaaacc gtaagcggcg gttttcagca agatccccgc
     3001 gaggtccacg gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc agccatgcag
     3061 cggaaccacc ggcattttga ctgcgaaggc gatgaagaag cccagcatca acagatattc
     3121 cacaccactg gacattggcg tattcagcag ctcttcatag ttgaaggtcc agacgccggt
     3181 cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc aacatcacca gaccactcgc
     3241 ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc gttttaccgt cagaggcttt
     3301 atgcccccac agtgcgatca ggaagtacat cggcaccagc atcatttccc agaagaagaa
     3361 gaacaggaac atgtcgatgg caaggaacac gccgataacg ccgcccagga tccacatcag
     3421 gttgaggtgg aagaagccct gatatttttc gatctctttc cacgaacata gtaccgccag
     3481 cacaccgagc agaccggtca gcacgaccat cagcagcgac agaccgtcaa tggcgagatg
     3541 aatagagata ccaaaacgcg ggatccacgg catgtcgaat tcagactgcc actgcggaat
     3601 tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg acagcgccag
     3661 cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa agcgttcggt
     3721 ctgccagcac aggaagccgc caataaaggg aattaatatt agccagggta gtaacatggc
     3781 gatctttatt ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt ctcacgacag
     3841 aaaattctct tcggactggg ggtagtgtcg gatgcgccgc gaaggcagct tatccgacct
     3901 acgttcttat atgcccaggg gcaatcccac aatccttaac tcaacgcagt accatcaaca
     3961 gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc agataaccgt
     4021 tctcgcttaa cagcagacct ttacctgcaa agcgggaaag aaccgccggg atgttcatca
     4081 ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ttgacgaaca
     4141 ctttgtcgta cagccagtca aagccccagg cgttgtacca ccaggtactc agcaggcggc
     4201 ccggcgcact gttggcgatg gaggtcacca gagtacgttt acccagccac agccaggctg
     4261 ccagcagaat gccgaccacc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc
     4321 cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc
     4381 caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca
     4441 cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt
     4501 agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg
     4561 ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc agcggcagtg
     4621 ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca
     4681 gaccgcccat cttgaagatg ttctgttcgt gatggcaggc cagaatgacg gaaccggatg
     4741 ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc gccgcatccc
     4801 atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga
     4861 gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg
     4921 ccccgacaat acccaccaga tgcagaactt ccggcgtcat caggaacagg ccgtgggtac
     4981 gggcgatcag gtagacaccc gcggttacca tcgttgcggc gtggatcagc gcggagacag
     5041 gcgtcgggcc cgccatcgcg tcagcaagcc atgtctgcaa cggcaactgc gcagatttac
     5101 cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa
     5161 agtgcgctgg tgccagttcc accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa
     5221 gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg aacgctttca
     5281 ttgccgctgc gccattcttc ggatcggtgt aatagaaacc gatcagcaga taggagcaca
     5341 ggcccacgcc ttcccagccg aggtacatca gcagcaggtt atcggcgaga accagaacta
     5401 ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg agagtagccc tcttcaccgc
     5461 gcatgtacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc accgagagca
     5521 tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg cctaccgaca
     5581 tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa tcaacgccga
     5641 taaaggcggt taccagcgcc gccaggccca cagagccaac gccgacgatc gccgaaacgt
     5701 tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa
     5761 tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg
     5821 gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag
     5881 gctgatggcg agaatgtaca tcacctgacc gtcggtctga ccccagtagc ttccggcgac
     5941 cacaaaggcc agcgcggagg cgttaatcat gatttccaga ccaatcagca taaacagcag
     6001 attgcgacgg ataaccagac cggttaagcc aagaacgaat aagattgccg cgaggatcag
     6061 tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta
     6121 cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga
     6181 cccgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta
     6241 atacccactg ctttagcact gattggcgta ccgtcgatac cctgatcgtt aacaccgagg
     6301 atggcgtaaa caatcaccac cagcatgatg gccgacaaaa ttgccgggcc aatccacacc
     6361 tgcggtttca gccactggcg ttcctgttcg atttctgaac cgcccaagtt gagcatcatc
     6421 accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg
     6481 gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg ccagcagcga aataatcagg
     6541 tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct
     6601 atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct
     6661 tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca
     6721 tacctgccat ccggtagaag ttatattccg ggtatttgcc cggaccggag atcagcagat
     6781 cctctttctc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta
     6841 actgaatcgc cgtggtcgga caggcttctt cgcacagacc acagaaaatg cagcgtgaga
     6901 agttaatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg
     6961 agatacagcc gaccgggcag gctaccgcac agaggttaca ggctacgcaa cgctcttcgc
     7021 cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga tagaccggct
     7081 cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac
     7141 tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc
     7201 ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg
     7261 caggcagatt ttccagccga aggacattac ctggtcataa cgcggacgcg gtaacgacgc
     7321 acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg
     7381 cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg cagagatggt
     7441 cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg aatattcaat
     7501 gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca
     7561 caccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga tgacgttcca
     7621 cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac
     7681 gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat
     7741 cgcacccagc agcgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac
     7801 cgccagacct gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca
     7861 acccggactg actggcacaa ttgcaaaggc cagcagcagc gaggtaaagg caatcatcgg
     7921 tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat
     7981 tttgatcatg tccgcaacca gctggagcga accgccccag ccaacacggt taggtccata
     8041 acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccacaggt
     8101 gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc
     8161 cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc
     8221 accggagcaa tgccggacat acccatcggc aagcccacct gccctgccgt cagtccttcg
     8281 gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac taaaggagac gcgtgtacct
     8341 gcgttcacac ccaacttcgc ggcatccgct gggttgagct tgatgtacgg ctgcggcata
     8401 cggctctgga agaccggagc acgctgtgac aattcatcgc tgccaaacag gtggtagtac
     8461 ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa
     8521 tccagaccgt tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg
     8581 ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc cggcgcccag
     8641 gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt taccttccat cgagaaggtg
     8701 aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg
     8761 gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat
     8821 gtcgcatccg gcgcagcatc tttgataccc gccagttccg ggattttcgc cacaacagcg
     8881 tcaattacat ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc
     8941 gagtgcagcc agcgccagct ttccagcatg acagttttgc tgtcgtaata agccggatcg
     9001 taaacctgga agaaacgttg ggcgcgacct tcattattaa ttaccgtacc gtcgctttca
     9061 gcaaagctgg cagcggaaag caccagatgg gcgttttcca tgatcgctgt gcgttgatga
     9121 tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcagaagcg
     9181 taacgatgca gatcgttttc caacaccacc accgcgtcgg cgcgtccggt ttccagttcg
     9241 gttaacgctt cttcaagcga accgccaccc ataatgccca gccccatgct gttgacggaa
     9301 cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta
     9361 gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc cggagataat caacggtttc
     9421 ttcgcacctg ccagtgcctg cacgatgacg tcgattttgt tttgcagctc aggttcgata
     9481 ccgtcaaccg ctggtgcgga gttatccagc gcatgggcga tggcaaaacc taaacgcgcc
     9541 tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg agtgtcatca
     9601 acgttggtaa caaacagcgg atgcttcgca cgttgaccga tgttgaggat tgccgcaatc
     9661 tgccagtcag ccactttctg tgctgcagcc atttcgcgcg ctttaccttt caccgcctga
     9721 cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc
     9781 gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc acgcagcact
     9841 ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct
     9901 tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaata
     9961 ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg
    10021 ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg atccttcagg
    10081 ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg
    10141 ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt caccggggct gatgttacag
    10201 ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa
    10261 cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac caggttaccg
    10321 gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgcg
    10381 ccataaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac acagcggtag
    10441 caggcgatgc agcggttcat ttcgtgagag atgaatggcc ccaaatcctg attacggtgg
    10501 gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacggt catatcctga
    10561 agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac
    10621 cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg
    10681 ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt
    10741 tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag
    10801 caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt gtccgctccg
    10861 ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc
    10921 tcggtcaggg accgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg
    10981 ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgattcccg cctcaaattc
    11041 ttcgcggaaa tatttgatgg cgctctgtaa cggctccact gcaccaggtg cgtgggcaca
    11101 gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg
    11161 ctggccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg
    11221 gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt tacgcaccag
    11281 cgacaccatg ttgatctcat ggtcaaccgc catcgccagc gccgtaccca gacggctgcc
    11341 cgctttaccg atactttcga attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc
    11401 agtccccgcg ccgcctggct gccaggcttt aaatttcaga ccatcacgca taccaccggc
    11461 gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca gtcccggatt
    11521 tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt
    11581 ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt
    11641 gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg
    11701 tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc cgcagatgta
    11761 gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca taatgttttt
    11821 gccaagcaag cccgcttcag tggcttcggc aatggcgcgg cgcagattta ctgccgcttc
    11881 gatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa acgcggagat
    11941 gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt ctttataggt
    12001 gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt
    12061 cggcatcagg ctccacttca ggccagtcga aaagcccgcg ccgccgcgcc ctttcagacc
    12121 agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc
    12181 cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt tgtcatcgcg
    12241 cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc
    12301 tccagcagtt cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc
    12361 atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt aaagcggcca
    12421 tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc ctgaataccc
    12481 tgatacccgt tgatatgaca gaccacgctg tcgcaataac ggatcacatg gcgaccaacc
    12541 ggctggcgga agatctgact gtagaacgtt gccacacctt cgacgtcgct tgccggaata
    12601 cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg ctgcttctga
    12661 acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc
    12721 tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc
    12781 tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa
    12841 tcaggtcaga caccaggctg ccgcggatcg ccgtcggaat ttgctgcaaa tgcgcataac
    12901 tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc aggtagtaac
    12961 tgttgatccc cttggtcgcc tcaatcatct ggaaagattc attggcaggc atcaccggcc
    13021 cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt
    13081 tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc atgttgttga
    13141 ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttcagc attacgcggg
    13201 tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt tcatagccag
    13261 aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg cccgcgccag
    13321 tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt
    13381 tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt
    13441 cgaggaactc acgcagcaga cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa
    13501 tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt
    13561 tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg
    13621 tcgagatgta cagcaggtgg ctgttgatac ggaacagttc ggagagcata acgcgaatga
    13681 cgttaacgcg atccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca
    13741 tttcgttaac gcagccgccg aggtattcaa tacggtcggt atacggaatg tagctgtgcc
    13801 aggactggcg ctcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc
    13861 agtcgacaat ctcttcgccg tcgagttgta aaacgatacg gaaagccccg tgcgccgacg
    13921 ggtggttcgg accgaggttg aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc
    13981 cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg gctttggtca
    14041 gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc tttacgcagc gggtgacctt
    14101 tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa
    14161 acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg
    14221 gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga
    14281 tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc
    14341 gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct
    14401 ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg
    14461 tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatta
    14521 ccggatcatc aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca
    14581 tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata
    14641 gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa atctcgtcag
    14701 gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca
    14761 tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttgc
    14821 cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc
    14881 ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat
    14941 cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt ggttccagca
    15001 tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt cctgcaacca
    15061 ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt
    15121 catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat
    15181 acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca
    15241 cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt
    15301 aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc
    15361 gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg
    15421 aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta
    15481 acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg
    15541 ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca
    15601 gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa
    15661 acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca
    15721 tcaggcaaca caggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga
    15781 tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac
    15841 tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac
    15901 aatcactgta gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa
    15961 ccttttgtca actttaacaa aagtttcttc acattagttt acataatatc aacaccatta
    16021 gcatttaatg ccctttcacc ccagatcctt gacgactcca ggataattag atgttgttga
    16081 atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc
    16141 gattcctccc cccaaaagag agtattttct tgatctgtga cacgcttttg ttattccata
    16201 acaaaaacgc agcaacaaat ttacgtattt tttaacatca ttgtagcagg tgattttttt
    16261 caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat aaacagtttt
    16321 gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg gggtttaaaa
    16381 cacccccagc ggctcgtttt ttacactatt gtctcaggaa ttatctatcg tccgtcgatt
    16441 actcgatatc cctttcaatc aacaatgaat catccccttc cggagcagac attgggctgt
    16501 attgccacgg attatggtag ctctccatcg cctgatagat cacctgggcc agctcattat
    16561 tactggacgg gtcatagcac agcaaatatt cggtatcagg tagcggcggt aagccatcta
    16621 ctccactcag cacacgcagg tccgggctca tcatctcaac cggccttgcc gtcacgccaa
    16681 gaccggattt cactgccgca cgaacggccg gaagcgtcga ggcgacataa gccagtcgcc
    16741 atggaatatc tgctttatta agcgtcgcca gcaccatatc gcgaaacgga cttggatcat
    16801 ccagcaatac aagagggatc ggctcgcctt tttgcaatac gtattccgct gcgcagtacc
    16861 agtgtgttgg cgaggtacgc aggttcaatg ccttaaacgc tgaaggacga tgggtggtta
    16921 ccatcaaatc cacttcctgg gattccagca tttctgccat ataggcatta cgtttaacgc
    16981 ggacatccag cgctaatttc ggataaaccg aactcacgcg atttaacagg aaaggtaaga
    17041 tcgtatcggc agattcatct gaagcaccga tagttaacac gccctgaaga ttactgaaca
    17101 ttaatgatga gcaggcctca tcattaaaac gcaggatttt cctggcgtaa ccaagaagtt
    17161 gaatgccatg ttcagttaac agtttgttgc gaccgtgacg agcgaacagt tctttcccaa
    17221 cgagttgttc cagacgctgc atttgctgac ttacggcgga ctgagtacga cacacagcgg
    17281 cagctgcggc agcaaaagtg ttcagatcgg caacagcaac aaatgttctc agcagatcga
    17341 ggtcgaggtt aattatcgga cgatttgcac ttatcatata ttatcactta ctggcggctc
    17401 atactgagct ggttaatgct gtgcacacac aaacaagcaa ttccatttgt aatgtgcctc
    17461 cctggcagtt tcatccggaa cccggacgaa agtaaaaatg catatgagtt gcactaaaaa
    17521 agcgactcac attgttccgt tataatgcct gaagtagatc acagaatata tcttcaggga
    17581 tcgcatatct attaagttac tcactctttt ctatttatga catgcgcgtg tttgtataaa
    17641 tgtaaatgtg agtccttgtt ccactctcgt gcagcatcgc tggtcatacg cgaacacgta
    17701 caaacagcaa tggtgaagcg gcatcaagag cagggatccg tcatttatgc gagcatttta
    17761 ccccaaaaac cttttattta taagggtcat tgcgaattat ctgatgcaaa gttatgttat
    17821 gttaggcaaa gtaatcttct attattaata agcacatcaa aacctttttg aatattaaat
    17881 aataattaat tagcatcatc ctcattcatt aattccgctt aacaatagtt tcacacctta
    17941 cccccattgc gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat
    18001 catcatcgcg tttcattagt gaattcttaa atgaggcatt ttcacacaat tatcttacag
    18061 ataaaaaaac cagacttgca attaagaatt agaacaattc actatataac attgcatgta
    18121 aagcatatac acctcattat tttgtcatta ttaagttatt aacagcacaa tcgagccttc
    18181 ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt
    18241 tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt
    18301 cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc
    18361 tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca
    18421 acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc
    18481 tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt aaagccatca
    18541 tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca
    18601 atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa
    18661 tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt tccagcggta
    18721 aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc
    18781 gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat aacccaaccg
    18841 gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc
    18901 tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct gagcatcact
    18961 caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg tcgaaaacgt
    19021 accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca
    19081 aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg
    19141 cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc
    19201 ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac gatattccgg
    19261 gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac
    19321 gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc
    19381 tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc
    19441 taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc ctttctggtt
    19501 atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg ttatcccgcc
    19561 acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc
    19621 ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg tgcggacgga
    19681 aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa
    19741 aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca
    19801 cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaattcgca
    19861 aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg tcgatatggt
    19921 tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga
    19981 agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga
    20041 actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga
    20101 agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca gcttccatct
    20161 ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct gcgtcgcatc
    20221 aggcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc
    20281 atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt cacaaagtca
    20341 ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga caccggtgtc
    20401 ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg cgacactccc
    20461 atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag
    20521 ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat atgtggccca
    20581 taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc cgttttctgt
    20641 aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat
    20701 ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata
    20761 gcggcaacag gattaggaat ttcatctgtc agcattaacg ccaccattag caccagacag
    20821 aaaatggcat ggggtgcctg gctgtgcgcg gtcgatgctt cactcacctc ttccggtaag
    20881 ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc
    20941 acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc
    21001 acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa accaatttcg
    21061 cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca
    21121 tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca
    21181 ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc
    21241 gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt
    21301 tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga
    21361 cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat
    21421 tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta
    21481 tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc
    21541 aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg cagtaattca
    21601 ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct aatcagcccg
    21661 gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt
    21721 aacaccacgg gaataaagat agcgacaacg ccggttgaac tcataaacgc gccaagcccc
    21781 gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc cactttgacc
    21841 agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac
    21901 aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc ggggaccgtc
    21961 agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact
    22021 ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca aatcaattca
    22081 ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca taaaaaagcc
    22141 ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg
    22201 gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc
    22261 gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa
    22321 gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct
    22381 gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta
    22441 caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct accggcatgg
    22501 agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg agtaaggcga
    22561 ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccagacgcg
    22621 taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc aaagaggtga
    22681 tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg
    22741 cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat
    22801 caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt
    22861 tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga
    22921 tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc aaaatgggta
    22981 tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg
    23041 gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta
    23101 gaccaggaaa catgcaacgc atgatacatc tccataatgt cgatgatagt gcggcaggtt
    23161 tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc gtaaccacgc
    23221 tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc
    23281 atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc
    23341 tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta
    23401 atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga aggcgcgctt
    23461 aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc
    23521 agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag ggggtaatgg
    23581 cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag
    23641 tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc agagagtaaa
    23701 tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc
    23761 gacaaagacc ggggcaaggc gtttttccag tggccacgtc tttgagtaat gctgtccgcg
    23821 gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa
    23881 cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa
    23941 aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag
    24001 tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa
    24061 aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt
    24121 taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac gtttttttag
    24181 ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg
    24241 tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg
    24301 tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa
    24361 acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa
    24421 tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat
    24481 cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg
    24541 tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga
    24601 agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc
    24661 gttccaccag actatgccgg aagagtctta cctctacgcc ttgccgtaca acctgtacaa
    24721 agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga
    24781 agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg
    24841 caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggtct
    24901 gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat
    24961 cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa
    25021 agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa
    25081 ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa
    25141 atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg tattcactgg
    25201 tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct
    25261 gggctttgaa gttgattatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat
    25321 caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc
    25381 gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt
    25441 tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg
    25501 ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca
    25561 atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt
    25621 ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc
    25681 gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg
    25741 ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg
    25801 gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa ctacgaaatc
    25861 gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa
    25921 cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa aaacaccaac
    25981 atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc
    26041 ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg
    26101 gcgaagctgc aagaatccag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac
    26161 ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa
    26221 ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac
    26281 atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg
    26341 ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct gctgctgact
    26401 ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc
    26461 ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct gcagagcttc
    26521 aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga atacgttgct
    26581 aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg cagccgtcgt
    26641 ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc gggcaaacgt
    26701 atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa
    26761 cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg
    26821 tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa
    26881 agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga aaccgttgcc
    26941 cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt
    27001 gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag
    27061 ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg
    27121 gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc tgaacagctg
    27181 gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga accgcgcgtt
    27241 gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc
    27301 gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag
    27361 tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca
    27421 ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg
    27481 gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaaaccg
    27541 gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact
    27601 gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg cgctgcggat
    27661 atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg cattaatcct
    27721 ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca ctttgcatac
    27781 ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa
    27841 ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta
    27901 accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat
    27961 caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgcattctg
    28021 actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa
    28081 cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa atacgggaca
    28141 gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt
    28201 acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt
    28261 ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga
    28321 gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac
    28381 agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc cagttcgtgg
    28441 atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt gctttctggc
    28501 tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg
    28561 gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc
    28621 ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg tgactggctg
    28681 gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc
    28741 tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc
    28801 ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggattcatc ctttaccgaa
    28861 ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg
    28921 ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct caacagcatt
    28981 gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt gctcttccag
    29041 gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcgttaac tatgccgtta
    29101 ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcgttccag
    29161 tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat ggcaacgctc
    29221 ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct gcttggactg
    29281 ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta ccactaaaaa
    29341 tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca
    29401 tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca
    29461 tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa cttcactgac ttcatcttcc
    29521 tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac acggcaattt
    29581 ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat gcccaactct
    29641 tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac tacaccgcct
    29701 gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga
    29761 attttaccca tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc
    29821 cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc
    29881 cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct
    29941 cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata
    30001 ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataactcgcc
    30061 ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc
    30121 tgttctgcca ctggcaaatg ggtatgaccg tacaccagca catcgttctg gtttaaagca
    30181 ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt
    30241 aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc cacttcgctg
    30301 tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact
    30361 ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca
    30421 aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg ttccgtcgcc
    30481 ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa
    30541 aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag
    30601 tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg
    30661 ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc
    30721 agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat
    30781 cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc
    30841 cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca
    30901 ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca
    30961 ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc
    31021 aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg
    31081 ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc
    31141 ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag
    31201 cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat
    31261 agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac
    31321 ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg
    31381 acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg
    31441 attaaggtag acctggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc
    31501 aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc
    31561 ctctttgagt ctggtgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt
    31621 catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg
    31681 gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac
    31741 gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa caagtggctg
    31801 gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg
    31861 tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc agtcaagaac
    31921 tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc
    31981 ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt
    32041 aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc
    32101 gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta
    32161 tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg
    32221 cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct
    32281 ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga
    32341 cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca
    32401 tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca tagtgatcac
    32461 cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc tgggccatca
    32521 aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc ctcgggtgac
    32581 actatggcaa gggcttgccg atcaaagcaa cctcaacggc attgatgcgg taatcaacct
    32641 ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc gtctctgcca
    32701 aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgccagcg acacgccgcc
    32761 gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt
    32821 taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc gctgggaaga
    32881 gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa ccggcgtggt
    32941 actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc ttggcctggg
    33001 cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg atatggtcaa
    33061 cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta
    33121 cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggctat
    33181 tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag tactggtatt
    33241 aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta
    33301 cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc aaacatccgc
    33361 cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt ctggtgaaag
    33421 tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct cctgagcaat
    33481 acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat
    33541 ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg
    33601 gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta gctgtctgtc
    33661 attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg
    33721 ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt gcataatctc
    33781 gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata ttttgcgggc
    33841 tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca
    33901 accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg catcaagatg
    33961 gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt
    34021 actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt tcacagacca
    34081 caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttctagttt
    34141 aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat caataaaatt
    34201 ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt
    34261 tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca
    34321 cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt
    34381 ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat
    34441 actccggcag cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt
    34501 gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt
    34561 tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag
    34621 tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc
    34681 ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tccggttcca
    34741 tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat
    34801 atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc
    34861 gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccaaca
    34921 ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta
    34981 agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga
    35041 tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag ttaatgcagc
    35101 gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat
    35161 tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga
    35221 taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc
    35281 tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa
    35341 tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg
    35401 cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt agagtgcagt
    35461 atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata
    35521 atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca
    35581 tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc
    35641 gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag gaattcggtt
    35701 cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc
    35761 ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg
    35821 gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata
    35881 agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt
    35941 ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc
    36001 ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc
    36061 cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt
    36121 tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc
    36181 agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa
    36241 acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct ttcggcactg
    36301 ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga
    36361 taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcacca
    36421 tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag tccggcacgc
    36481 ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct
    36541 taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct
    36601 ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg
    36661 tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg
    36721 gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc
    36781 taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg
    36841 cagttcgtta tcttctttac gcaggcccat gccggtccct acaccaaaca gtttttcatc
    36901 tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc
    36961 gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata
    37021 aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc agtgttcatt
    37081 accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc
    37141 gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag
    37201 tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg atgacatgat
    37261 ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt tttcgacaaa
    37321 cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat cgaaaccaac
    37381 cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg
    37441 gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca gaaccagaga
    37501 gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat
    37561 ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc
    37621 ccgttagact tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat
    37681 agtgcaacaa acctagcgtg caccatgatg atcattccat caggtacagc ttcccagcga
    37741 cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtgc
    37801 cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcatcatctt
    37861 tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag
    37921 caaaggcgaa atctttacca gcaggttgct tgaggaatcc ttcgctagca gcaacttcat
    37981 cttgtaacgc agcatccaga cgccctgcag ccagatcgga atagaccaaa tcctggttgg
    38041 cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa gcttcctggg
    38101 ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt ggctgaatcg
    38161 gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg gagaaggcaa
    38221 tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg atttttttcg
    38281 ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ctaggtacat ttcacctgca
    38341 tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca cctttagcat
    38401 ctttcgacga gaacggcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg
    38461 ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg agaatcgact
    38521 tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt
    38581 ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt
    38641 aaatatgtta ataagacgtt gcattattgt cctgaagttg aagatagcag gtatagcggt
    38701 tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg
    38761 caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga
    38821 tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg atttatcaca
    38881 tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg
    38941 atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg
    39001 cagagtacca acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca
    39061 tcagtatagc tatggacaat gccggaaagg gttttgattg aacagggtaa aatcaccatc
    39121 cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc atcgtgcgtg
    39181 acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg
    39241 cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt
    39301 aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc
    39361 ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc gggagttgca
    39421 agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat gcatttcgag
    39481 attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta acgtatcgag
    39541 gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca
    39601 ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat cgttcagatc
    39661 ctggaaaatc accccgtcag caccgatgat ctggcgaatt tcatcaactt cgcgaccgtg
    39721 agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc
    39781 cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat
    39841 ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg
    39901 gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca gctgctggcc
    39961 cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt
    40021 gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga tcgggatcac
    40081 cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat
    40141 attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc gggcaaagta
    40201 tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa
    40261 ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa
    40321 gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac ggttctcgtc
    40381 aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga aagcaaccat
    40441 accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtggc
    40501 agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt tgtccagctc
    40561 gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt
    40621 ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag
    40681 cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc
    40741 agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg
    40801 agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc
    40861 atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc
    40921 ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct
    40981 ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct
    41041 ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca gctgtgattt
    41101 gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc
    41161 aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga
    41221 caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac caacgatcag
    41281 ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc
    41341 cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca
    41401 tgtcaccagc gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa
    41461 agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc
    41521 cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc
    41581 cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg
    41641 cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc cgacctacgg
    41701 ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa gactgtatta
    41761 attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca actcacccag
    41821 cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg taattttacc
    41881 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    41941 aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat aggctttacc
    42001 cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc
    42061 ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg
    42121 ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc
    42181 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg
    42241 ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca ccagcgggat
    42301 agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    42361 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    42421 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca
    42481 ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc
    42541 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    42601 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    42661 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    42721 gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    42781 taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    42841 cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc gccagcgccg
    42901 ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat
    42961 cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    43021 acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga
    43081 caattgccgg tttttcgctg cggaagatgc ctgctttctc gcgaccaata ctttcgcgat
    43141 ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcat
    43201 cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa
    43261 gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    43321 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccagca
    43381 attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc gaactgtaga
    43441 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    43501 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg ccaagacgcg
    43561 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    43621 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    43681 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    43741 tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg
    43801 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca
    43861 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac
    43921 tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    43981 caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    44041 cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    44101 gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    44161 gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct tcctgcgcct
    44221 gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg
    44281 cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca
    44341 gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    44401 agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc acaaggcttc
    44461 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    44521 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    44581 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    44641 taattcgttc aatccagctc attagggacc tttctgtctg aacctagttc gatgccagtt
    44701 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    44761 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    44821 ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat
    44881 agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt
    44941 accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt
    45001 ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt
    45061 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa
    45121 ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa
    45181 cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat
    45241 cagcatcagc accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc
    45301 tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca gaccggtttc
    45361 acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg cgactagctc
    45421 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    45481 tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat
    45541 gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    45601 gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    45661 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    45721 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    45781 cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa
    45841 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    45901 cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    45961 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat
    46021 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    46081 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc gcgtctttgc
    46141 gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct gcgtcagtac
    46201 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    46261 tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta ctgccgtcgt
    46321 actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac attagtacag
    46381 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    46441 gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca
    46501 gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga attcgttctc
    46561 ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgccgccag
    46621 cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    46681 gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat
    46741 acaacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca tattgaacgc
    46801 cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttcgcact
    46861 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    46921 aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg
    46981 gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    47041 cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca gtccgctgct
    47101 gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt
    47161 gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac
    47221 ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    47281 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccat
    47341 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    47401 aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc
    47461 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca
    47521 caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg
    47581 aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    47641 accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag cgtaatgcgg
    47701 ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg cccaataaac
    47761 ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag ggtatagcct
    47821 gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg
    47881 ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc
    47941 gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc gggcttcagg
    48001 ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc tttaaagagt
    48061 ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga gcggaaatca
    48121 ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct
    48181 tccagtcgca cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg
    48241 tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc
    48301 accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac
    48361 catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt aatgggtttt
    48421 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    48481 gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta
    48541 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    48601 gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg
    48661 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctaggca
    48721 gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    48781 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacaacaaag
    48841 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    48901 ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    48961 aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa
    49021 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    49081 ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc
    49141 gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc acaatgcgct
    49201 aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac agatctggca
    49261 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    49321 ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    49381 ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    49441 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc
    49501 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat
    49561 gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    49621 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta
    49681 accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga tcgccccggc
    49741 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac
    49801 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    49861 ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    49921 ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga gcagcaacaa
    49981 gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg
    50041 catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa
    50101 gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    50161 aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct
    50221 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    50281 ccacgccagt aagggtaata ccattgtggt gagccagtgc ggcaaaaccg taatggctat
    50341 gaatcaacag gccaagcggc gcaccaacag cgagggcacc gtaaatcgcc attccgttcc
    50401 atgacatcac tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc
    50461 ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa
    50521 atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac
    50581 cacaagctaa catcccctga agcgccgaac gttttgcgcc atattgatcg gccagtcgcc
    50641 cggcgtaacc acgcgtcagc accgtagcca gaaactgaat cccgacggca atgccgacca
    50701 tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcaacc
    50761 ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaactggcag
    50821 aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg
    50881 cgaggagtat ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggattta
    50941 acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aactggtggc
    51001 gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc
    51061 cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccggcct
    51121 actgcgaatt aatctttcag cttgcgcatt accagcgtgg cgttggtgcc gccgaagccg
    51181 aagctgttag acataacagt ggtcagttcg cgatcggtcg tttcggtcac gatgttcaga
    51241 cccgcagcct gctcgtccag ctcttcaatg ttgatgctcg gggcgataaa gccgtgttcc
    51301 agcatcagca gagagtagat agcttcctgc acacccgcag cgcccagtga gtgaccggtc
    51361 atggctttgg ttgcagaaat cgccgggctc ttatcgccga acacttcacg gatagctgcc
    51421 agctctttca cgtcgccaac cggagtcgaa gtaccgtgag agttcaggta atcgattggg
    51481 gtatcaacgc catgcatcgc catctgcata caacgtactg cgccttcgcc agacggagca
    51541 accatgtctg caccatcaga ggttgcgccg tagccaacga tttcagcata gatgtaagca
    51601 ccacgcgcca gcgcgtgttc cagctcttca accactacca taccgccgcc gccagcgata
    51661 acgaaaccgt cacggtgagc gtcgtaagta cgggaggctt tttccggggt gtcgttgtat
    51721 ttagtagaca gcgcacccat cgcgtcgaat tcgcaggcca tttcccagca cagctcttcg
    51781 ccgccgccag caaacacgat gtcctgtttg cccagttgga tctgctctac tgcgttaccg
    51841 atacagtgtg cggaagtcgc acacgcggag ctgatggagt agttaacgcc atgaatttta
    51901 aacggcgtgg cgagacaggc agaaacgccg gatgccatcg ctttagtgac cacatacggg
    51961 ccaaccgctt tcaggccgcg cgggccgcgc attgcgtcag cgccgaacac ctggaaacgc
    52021 ggggaaccgc cgccggaacc tgcaatcagg ccaacgcgcg ggttattctg gtaagcttcc
    52081 ggagagaggc ccgcatcagc gattgcctgc tccatagaaa ggaatgcata aatggatgcg
    52141 tcgctcataa agcgcacaac tttgcggtca atgaggccag tggtatccag ttttacgttg
    52201 ccccagacgt ggctacgcat gccggaatcc ttcagctcct gagagaaagt gatccctgaa
    52261 cgtccttcac gcagagatgc caggacttcc tgctggttat taccgatgct ggaaacaatg
    52321 cccaggccag taatcactgc acgtttcatt caatacctct gtaagtcgca catagagtaa
    52381 gtttcgaatg cacaatagcg tacacttgta cgccgaacaa gtccgatcag ccatttaata
    52441 gagaaatttg cgcagcctta cacacatcgc taagatcgag ccaccgcctg taagacgagt
    52501 aacttacgtg aaacactact ccatacaacc tgccaacctc gaatttaatg ctgagggtac
    52561 acctgtttcc cgagattttg acgatgtcta tttttccaac gataacgggc tggaagagac
    52621 gcgttatgtt tttctgggag gtaaccaatt agaggtacgc tttcctgagc atccacatcc
    52681 tctgtttgtg gtagcagaga gcggcttcgg caccggatta aacttcctga cgctatggca
    52741 ggcatttgat cagtttcgcg aagcgcatcc gcaagcgcaa ttacaacgct tacatttcat
    52801 tagttttgag aaatttcccc tcacccgtgc ggatttagcc ttagcgcatc aacactggcc
    52861 ggaactggct ccgtgggcag aacaacttca ggcgcagtgg ccaatgccct tgcccggttg
    52921 ccatcgttta ttgctcgatg aaggccgcgt gacgctggat ttatggtttg gcgatattaa
    52981 cgaactgacc agccaactgg acgattcgct aaatcaaaaa gtagatgcct ggtttctgga
    53041 cggctttgcg ccagctaaaa acccggatat gtggacgcaa aatctgttta acgccatggc
    53101 aaggttggcg cgtccaggcg gcacgctggc gacatttacg tctgccggtt ttgtccgccg
    53161 cggtttgcag gaagccggat tcacgatgca aaaacgtaag ggctttgggc gcaaacggga
    53221 aatgctttgt ggggtgatgg aacagacatt accactcccc tgctccacgc cgtggtttaa
    53281 tcgtacgggc agcagcaaac gggaagtagc gattatcggc ggtggtattg ccagcgcgtt
    53341 gttgtcgctg gcgttattac ggcgcggctg gcaggtagcg ctttattgcg cggatgaagc
    53401 ccccgcgctg ggtgcttccg gcaatcgcca gggggcgctg tatccgttat taagcaaaca
    53461 cgatgaggcg ctaaaccgct ttttctctaa tgcgtttact tttgctcgtc ggttttacga
    53521 ccaattaccc gttaaatttg atcatgactg gtgcggcgtc acgcagttag gctgggatga
    53581 gaaaagccag cataaaatcg cacagatgtt gtcaatggat ttacccgcag aactggctgt
    53641 agccgttgag gcaaatgcgg ttgaacaaat tacgggcgtt gcgacaaatt gcagcggcat
    53701 tacttatccg caaggtggtt ggctgtgccc agcagaactg acccgtaatg tgctggaact
    53761 ggcgcaacag cagggtttgc agatttatta tcaatatcag ttacagaatt tatcccgtaa
    53821 ggatgactgt tggttgttga attttgcagg agatcagcaa gcaacacaca gcgtagtggt
    53881 actggcgaac gggcatcaaa tcagccgttt cagccaaacg tcgactctcc cggtgtattc
    53941 ggttgccggg caggtcagcc atattccgac aacgccggaa ctggcagagc tgaagcaggt
    54001 gctgtgctat gacggttatc tcacgccaca aaatccggcg aatcaacatc attgtattgg
    54061 tgccagttat catcgcggca gcgaagatac ggcgtacagt gaggacgatc agcagcagaa
    54121 tcgccagcgg ttgattgatt gtttcccgca ggcacagtgg gcaaaagagg ttgatgtcag
    54181 tgataaagag gcgcgctgcg gtgtgcgttg tgccacccgc gatcatctgc caatggtagg
    54241 caatgttccc gattatgagg caacactcgt ggaatatgcg tcgttggcgg agcaaaaaga
    54301 tgaggcggta agcgcgccgg tttttgacga tctctttatg tttgcggctt taggttctcg
    54361 cggtttgtgt tctgccccgc tgtgtgccga gattctggcg gcgcagatga gcgacgaacc
    54421 gattccgatg gatgccagta cgctggcggc gttaaacccg aatcggttat gggtgcggaa
    54481 attgttgaag ggtaaagcgg ttaaggcggg gtaatctgct ctcgcagtgt ttgccggatg
    54541 cggcgtgaac gccttatcca acctacgtgt gacgtgtagg cctgataaga cgcgataagc
    54601 gtcgcatcag gcatggtgct ccagacgccg caacttacag ctgtgacgcc tgctgaaaca
    54661 ggttttccca catatcggtc accagcgcct ggtcacgcgg cgacaattca ccggcaccaa
    54721 tggctttttc cagactctgg ctaacggtcg tatgcaccgc ctgagcggag tggtcatcac
    54781 cactttccag ttctgcgatg gctaatgtca ggtggccacg caaataccca ctggcaaaca
    54841 actcatcatc actggcatgg tcaaccatgc cgtcgattaa tgccagaatg cgtgattcaa
    54901 actccgcgat catcttcttt cctcattgta aaacgtggcg ctggcttcag ttgagcgctt
    54961 ccggccacgg gaactgttcc gccgtaagtt ccggcgtgtg ataataattt tgtagtgctt
    55021 taatgaagcg tgccgggcgt tccggaatgc catttgtcag ataatccatc acctgcgcat
    55081 gtacccgccg ttgaaacacc acacgatccg gttcgaaatc cccttccaga ttgtcgcaac
    55141 tgacgttaaa cggatatcca gccgccacgc agaataacca gtcgaacgcc tgcggcttca
    55201 cttcaacatc ttcaaactgg ctttgcgtct gggcatcgcg tccgtccggg caataccagt
    55261 agccgaaatc aaccagctca cggcgcgctt ttccggcaat acaccagtgc gaaatctcat
    55321 gaattgcgct ggcataaaag ccatgagcaa agacgattcg gttatacggt acttccgcat
    55381 cagcaggaag atagatcggt tcgtcgtcgc ctttaatcag acgggtatta aattcatcgg
    55441 caaagcagct attaaaaatt tcaatcaact gctcgtagtg gtgtgtactg ttcattagtt
    55501 catccccaac cagtggagga tctcctgtcc gtggctatcg taaagtaatt tggcactcat
    55561 caccgccgag acgataacaa tcatcgggcg gatcagcttt tgtcctttgc tcaacaccaa
    55621 ccgtgaaccc atgcgcgcgc cgaggaactg ccccaccagc atgacaaagc ccgtcgccca
    55681 aatcactttg ccgccgagaa taaacagcag cagaccgcca atgtttgacg ttgcgttgag
    55741 taatttggcg tgagccgtgg ctttggcgag gttgaatccg cacagcgtaa cgaaggccag
    55801 cgcgtaaaac gatccggcag ccgggccaaa gaatccgtca taaaaaccga cgcagccacc
    55861 ggcaattaac gcgaagggta aaccgtacat ccggcgctgg cgatattctt cacccagctt
    55921 tggcatcagc aagaaataga ggccgataca aatcaccaga atgggcaaaa tctgccgtaa
    55981 gacatcagcc tgaacgtact gtaccaacag tgcgccgctc attgagccga caaaggtcat
    56041 ggcaatattg agtttctgat cgcttaaact aaccactttg cggcgaataa agtagatagt
    56101 ggcggaaata gagccgccgc aggcttgcag tttattggtt gccagcgcgt tagcgggaga
    56161 cattcccgca gccatcaatg ccggaatggt gagtaaccca ccgccaccgg caatcgagtc
    56221 gataaatccc gccagcatgg cgacaaaaaa gagaactccc agcaacagcg gagaaaccat
    56281 aaacaggcta ttaaacgttt ccattagatc acgtgctcat ccagtagcgc ctggcaggaa
    56341 ggcggcaacg gaggcggtgt cttcttctca ggctttgttg ttcccggttt tggaggttca
    56401 aaccagcttt gcagttctgc cccgcaacca tcgcctgatg gcggtaaagg ttgatcttca
    56461 cactccagac tatcggcagg gcaacgtaat cgtacatgca tatgcgcgcg atgctggaac
    56521 cagggtcgca ctttgcgcaa ccagtcgcga tcggtgcccg catcaaggca aagttgttgt
    56581 ttaatcgccg gattaacaaa aatgcgcgtg acgtctttgt cctgggcggc gagtttgatc
    56641 aagctgaaaa tttctggctt ccacagcgtg gagacaacgt gtttaccgtc gcgggaaact
    56701 aagtccagtg cttgcgggcg caagagctgc gcggaggtcc agcgagtttt cggcagttgc
    56761 agaaagatat cgacatccag tccggtctgg tggctggcat gaccgccgtt gaaacgccca
    56821 ccagcgggca tccccatatc gccaatcagc accgtaccca tgcccagatt gctcacttgg
    56881 ctactcagac gctggataaa catcaccaga tccgggtgac cgaaatagcg acgctgatcg
    56941 gtacgcatga cctgataatg ttcggactgt atcggcaacg tgtcagcgcc gacaatacag
    57001 ccattagaaa aactgcctat cgattgtgcg ctacccggca caggttgggt tattttttgc
    57061 cacggcgtcg ctgccaggct agcgctactg gcaagcagag ccagcagcgc aatcgcggtt
    57121 ttgttcattt tttaccagcg tggaatatca gtcttcacat cggcattttg cgcccgctgc
    57181 cgtaacaggt gatccattaa aacgatcgcc agcatcgctt cagcgatcgg cactgcacgg
    57241 atcccgacac agggatcgtg acggcctttg gtgatcatct caacttcttc gccaaagcgg
    57301 ttaatagtgc gccccggcac ggtaatgctg gaggtcggtt tcagcgccat atgagcaatg
    57361 atttgctgcc cgctgctgat accgccgaga atgccgcccg catggttgct ctggaaaccg
    57421 tctttggtga tttcgtcgcg gttctggctg ccgcgcagcg ccactacgtc aaaaccgtcg
    57481 ccaatttcca cgcctttcac cgcgttgatg ctcatcagcg catgggcgat gtcggcatcc
    57541 aggcggtcaa agaccggctc gccaagtccg gcggggacac cactggcaac aacggtgact
    57601 ttcgcgccga tggagtcgcc ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg
    57661 attttgtcgg ggtccgggca gaagaacgga ttttgttcaa ccagcgacca gtctttgatt
    57721 tccagcggaa tgtcgcccat ctgggtcagg cagccgcgga tttcaatacc aaatttctcg
    57781 gcgagatatt ttttggcaat cgcccctgcc gccacgcgca tggcggtttc gcgggcggaa
    57841 gaacgtccac cgccgcgata atcgcgcaga ccgtattttt gttcgtaggt gtaatcagca
    57901 tggcctggac ggaagacgtc tttaattgcg ctgtaatcct gagaacgctg gtcggtattt
    57961 tcgatcaaca agccaatgct ggtgccagta gtaacgcctt caaaaacacc ggagagtatt
    58021 ttgacctgat ccggctcgcg gcgctgggtg gtatagcgcg atgtcccagg gcgacgacga
    58081 tcaaggtcat gttgcaggtc cgcttccgtc agcggaatgc ctggcggaac accatcgacg
    58141 atgcagccga gcgccagccc gtgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca
    58201 attgtgtttc cagccatcac ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt
    58261 ataaatcgcg aaatgttctc gtgcggcaat aagctgctct ttagtgagca taaacacacc
    58321 atcgccgccg ttatcaaact ccagccaggt gaacggaaca tcgggatatt gttccataag
    58381 atgtaccatg ctgttgccca cttcacaaat caacacgcca tcatcagcaa ggtaatctgc
    58441 cgcgttaccg agaatgcgac acgtcagttt caggccgtcg gtgccagatg ccaggcccag
    58501 ttccggctcg tgacggtatt cgtttggcag gtcggacata tcttccgcgt cgacatacgg
    58561 cgggttagtg acaatcagat cgtattgcac tttcggcaaa tcacggaaca gatcggaacg
    58621 aatcggaatg acattgtgga tcagaccgtg ttcttcgatg ttctgttcgg caaccgccag
    58681 cgcgtcggga gagatatcta ccgcgtcgac ttccgcttcc gggaaggcat aagcacaggc
    58741 aatggcgatg cagccgctac cggtacacat atctaaaata tgctgcggtt gcttgctgat
    58801 gagtccggca aatttattgt tgatcagttc accaatcggc gagcgcggca ccagcacgcg
    58861 ttcatcgacg taaaattcat gaccgcagaa ccaggctttg ttggtcaggt aagccaccgg
    58921 aatgcgctcg ttgacgcggc ggatcacgcg ttcaacaata cggtgttttt cgctggaggt
    58981 cagacgcgcg gtgcgcatat cttccgggat atccagcggc aggtagagcg atggtaacac
    59041 cagctgtacg gcttcatccc actggttatc ggtgccgtga ccgtaccaga tatttgccgc
    59101 gctgaagcgg cttaccgacc agcgcaacat gtcctgaatg gtttgcagct catttactgc
    59161 ttcatcaacg aaaattttat ccacgtattc ctccagggca tgatcgcaat aatttcggcg
    59221 gctagtttgc catgaagatg acgataaatc agcattcacg cgcggtgagt gaggaaaaat
    59281 acgtttaaaa cgatcgattg tgctacgagt cgggtaaact gtaggaaaat tagaaataga
    59341 gacagataaa tgaaaaagaa aacaacactt agcgaggagg accaggctct gtttcgccag
    59401 ttgatggcgg ggactcgcaa gattaagcag gacacgattg tccaccgacc gcagcgtaaa
    59461 aaaatcagcg aagtgccggt gaaacgcttg atccaggagc aggctgatgc cagccattat
    59521 ttctccgatg agtttcagcc gttattaaat accgaaggtc cggtgaaata tgttcgccca
    59581 gatgtcagcc attttgaggc gaagaaactg cgccgcggcg attattcacc ggaattgttt
    59641 ttggatttac acggtctgac gcagctgcag gccaagcagg aactgggcgc gttgattgcc
    59701 gcctgtcgcc gcgaacatgt gttttgtgcc tgcgtgatgc atgggcacgg gaagcatatt
    59761 ttgaagcaac aaacaccgct gtggctggca caacatccgc atgtgatggc atttcaccaa
    59821 gcaccgaaag agtacggcgg tgatgcggct ttgttggtgt tgattgaagt ggaagagtgg
    59881 ttgccgccgg agttgccctg aatgctggca gcaaatgccg gagcagcgta aactccttat
    59941 ccggcctaca aatatgtgat aattcaataa attgcaattc acttcgtagg cctgataaga
    60001 cgcatcagag caatactacg aacttcagtt cattcaatac atactgaaaa cgacacgtcg
    60061 aatcaatcgg ttgcccacta ctggtattca cattcaccac aatatcgtgt agtactttat
    60121 ccgttaccgg atgacgagtt ggcgtaaccg ttaccgttga tttatcacaa ccgtaagagg
    60181 taaaggttaa caattctgat accgcgcggc agtatgctcc tctgccatca ttatcagaaa
    60241 aattactgcc atcacagagt ctgccgcctt ccatcaggta ggtcgaaagc gtggtggtca
    60301 ctgttgaccc cgtggactga attcgtaacg ccttattatg gctgacataa ttttcactcc
    60361 aggagatatc gtatgaagcg accggtatcg ttgggcatat tgtgttatgc actgtagggg
    60421 ccgtatagct tccaaatccg ggtaaatctg tataggaata aaaggatgcg atccaagggc
    60481 aaccataatc atcagatgca acggtaattt taaacgtcat atatgcacaa cgaaaaattt
    60541 tcgtatctat gccagggcaa tttgagttat taattgtatc aaagtcagtc agtaccaaag
    60601 ttggagctga acccgaccca gaccaggaaa taacccgcaa cgtatcgcct ttatacgcaa
    60661 cagaatcata tttcccatat gcaacctcct ttggcgttat caccgtaaca ttgaaaacac
    60721 tgctcgctac agcaccggtt accttgtcca tggcataata aactctcaca ctgtcaaaac
    60781 tcgcactcat aagtttatct ccagcataac tcttagatgc gccgacaaca ctcagaacga
    60841 gcagcattag gctcgcgaaa attatggtcc attttttcat ttcagaaata ctcaaaggtt
    60901 gccgtcactg tggcactaaa gtctccggac gttacatctc gcccgctctt tgcctgtaac
    60961 caggtattaa aatttaggct gttatttccc tgattcagcg ggagcgtgac gcctgttgta
    61021 ttgttgactg gcaccgatgt gcctgccgca gtttcaatac caatcccagc gccggatgct
    61081 gatgacgagg agtcaaaggc cagaaagccg ggtagagcac tatcttctgt tcctgtcaac
    61141 gttactttga cattaaacaa tgtagaacta tgacaatctt tcaattgaaa caccaatggc
    61201 actcgctctg actgcccaag gttcattaaa tcgctggcgg caatggcagg aaaatgtact
    61261 tctgccaact ggctaccatt aatcactggc gtacacgatt tactgattaa attgccggta
    61321 aagtgcaaat tatctcctgc tattgtggtt ccactgccca gcaacatcag gctactaata
    61381 aaaaacaacg tacgcttttt cttcattggt aatctacctg taacgtggcc caggcctcaa
    61441 agtccgcctc tggcagaacg acaccactct tttttactgg cacagccgtt aacaccggta
    61501 aatcggtttc attcaccaca agtggcgtat tgatcgtaaa actctgccct gcctgtttga
    61561 gctgaatgcc taaaccagtg atattgctgc ttactgcagc attatcaaaa tcgcttggtg
    61621 tgccactcca gctcaaggtc atttccatta atgggtcgcg aaccgccgta tcgcaggtaa
    61681 tggaatacgg cacggtttgc tcataattgc tgccattaat gtcatcaata agcacatcct
    61741 gaaaagttat ttcaatggtt tgagcgttgc taatcgtgca gttaggcggc tgcactaaat
    61801 aaccgtgaaa agagacgttt gagagtgcca tcgagcaaaa gccccacagc aaaataagcg
    61861 agatccgttt cattggtaat ccaccacaat tgtggcactg gcgttaaatt cagctgccgc
    61921 taacgttgta ccactctgtt tcaccggcac agcttccaga acgggaactc ccgaggaaaa
    61981 attaaatggc aaccatgagc cagaggttaa gtcgagtatt gtgtgatcac tacttttctg
    62041 aacccgaata ccaaacccag gaatacccgt gctgataact gtttcaccgt taatcacgac
    62101 cgttgtcgcc tgcatctgta gacgcaggtc atccattgcc agattgttac aaacaagcga
    62161 gtagtcaatg ggacgtttat aatcaacccc attgatctta ttaatgaata cattaccaaa
    62221 ctcaactgcc gcaccattta ccgtacaagg aggaggggca tcgacaagca cccgtaaatt
    62281 catacttact gacttactcg ctgcaactgc aaagccgcta ccaacacaac atatcagtaa
    62341 cccagttacc gcccagacag aacaagatcc tttcatactg gctctcccgg cctcagcctt
    62401 tgcggctctg ctcttcatc
//
LOCUS       DADKXM010000009        61550 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.009, whole genome shotgun sequence.
ACCESSION   DADKXM010000009 DADKXM010000000
VERSION     DADKXM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.009"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JQR05_000556"
     CDS             complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JQR05_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAY5322796.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQLRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDSD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JQR05_000557"
     CDS             complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JQR05_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY5322797.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVM
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2057..3460)
                     /locus_tag="JQR05_000558"
     CDS             complement(2057..3460)
                     /locus_tag="JQR05_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY5322798.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JQR05_000559"
     CDS             complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JQR05_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY5322799.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JQR05_000560"
     CDS             complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JQR05_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY5322800.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4198..5577)
                     /gene="pta"
                     /locus_tag="JQR05_000561"
     CDS             complement(4198..5577)
                     /gene="pta"
                     /locus_tag="JQR05_000561"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY5322801.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQLVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELALRAPARVVFPDALDQRVLKAAQYLHQQGL
                     ATPILVANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLAHAGEKTPPDAL
                     EKLTDPLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLP
                     QYSGPALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHP
                     CVANVQQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLE
                     AGNIGYKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNR
                     ESSLQTLVE"
     gene            complement(5574..6275)
                     /gene="eutQ"
                     /locus_tag="JQR05_000562"
     CDS             complement(5574..6275)
                     /gene="eutQ"
                     /locus_tag="JQR05_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY5322802.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(6250..6729)
                     /gene="eutP"
                     /locus_tag="JQR05_000563"
     CDS             complement(6250..6729)
                     /gene="eutP"
                     /locus_tag="JQR05_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY5322803.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(6742..7077)
                     /gene="eutS"
                     /locus_tag="JQR05_000564"
     CDS             complement(6742..7077)
                     /gene="eutS"
                     /locus_tag="JQR05_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY5322804.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(7370..9649)
                     /gene="maeB"
                     /locus_tag="JQR05_000565"
     CDS             complement(7370..9649)
                     /gene="maeB"
                     /locus_tag="JQR05_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY5322805.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            9938..10888
                     /gene="tal"
                     /locus_tag="JQR05_000566"
     CDS             9938..10888
                     /gene="tal"
                     /locus_tag="JQR05_000566"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY5322806.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            10908..12911
                     /gene="tkt"
                     /locus_tag="JQR05_000567"
     CDS             10908..12911
                     /gene="tkt"
                     /locus_tag="JQR05_000567"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY5322807.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     NPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(13014..14057)
                     /locus_tag="JQR05_000568"
     CDS             complement(13014..14057)
                     /locus_tag="JQR05_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY5322808.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(14183..14758)
                     /gene="nudK"
                     /locus_tag="JQR05_000569"
     CDS             complement(14183..14758)
                     /gene="nudK"
                     /locus_tag="JQR05_000569"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY5322809.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(14826..16805)
                     /gene="aegA"
                     /locus_tag="JQR05_000570"
     CDS             complement(14826..16805)
                     /gene="aegA"
                     /locus_tag="JQR05_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY5322810.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLAFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMRLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAERRHAAQGIIDWLGVKSVKSH"
     gene            17010..18722
                     /gene="narQ"
                     /locus_tag="JQR05_000571"
     CDS             17010..18722
                     /gene="narQ"
                     /locus_tag="JQR05_000571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY5322811.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDFPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAISCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEGKGNT
                     PAEV"
     gene            18924..22037
                     /gene="acrD"
                     /locus_tag="JQR05_000572"
     CDS             18924..22037
                     /gene="acrD"
                     /locus_tag="JQR05_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY5322812.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(22136..22195)
                     /gene="ypfM"
                     /locus_tag="JQR05_000573"
     CDS             complement(22136..22195)
                     /gene="ypfM"
                     /locus_tag="JQR05_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY5322813.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            22576..22932
                     /locus_tag="JQR05_000574"
     CDS             22576..22932
                     /locus_tag="JQR05_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY5322814.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            22936..24063
                     /gene="dapE"
                     /locus_tag="JQR05_000575"
     CDS             22936..24063
                     /gene="dapE"
                     /locus_tag="JQR05_000575"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY5322815.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            24091..24291
                     /locus_tag="JQR05_000576"
     CDS             24091..24291
                     /locus_tag="JQR05_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY5322816.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(24401..25099)
                     /gene="ypfH"
                     /locus_tag="JQR05_000577"
     CDS             complement(24401..25099)
                     /gene="ypfH"
                     /locus_tag="JQR05_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5322817.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(25173..27188)
                     /locus_tag="JQR05_000578"
     CDS             complement(25173..27188)
                     /locus_tag="JQR05_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY5322818.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(27203..28066)
                     /locus_tag="JQR05_000579"
     CDS             complement(27203..28066)
                     /locus_tag="JQR05_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY5322819.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNHLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(28234..28947)
                     /gene="purC"
                     /locus_tag="JQR05_000580"
     CDS             complement(28234..28947)
                     /gene="purC"
                     /locus_tag="JQR05_000580"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY5322820.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(29160..30194)
                     /gene="bamC"
                     /locus_tag="JQR05_000581"
     CDS             complement(29160..30194)
                     /gene="bamC"
                     /locus_tag="JQR05_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY5322821.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(30211..31089)
                     /gene="dapA"
                     /locus_tag="JQR05_000582"
     CDS             complement(30211..31089)
                     /gene="dapA"
                     /locus_tag="JQR05_000582"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY5322822.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNVSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            31235..31807
                     /locus_tag="JQR05_000583"
     CDS             31235..31807
                     /locus_tag="JQR05_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY5322823.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKSTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            31807..32277
                     /gene="bcp"
                     /locus_tag="JQR05_000584"
     CDS             31807..32277
                     /gene="bcp"
                     /locus_tag="JQR05_000584"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY5322824.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            32530..33147
                     /gene="hyfA"
                     /locus_tag="JQR05_000585"
     CDS             32530..33147
                     /gene="hyfA"
                     /locus_tag="JQR05_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY5322825.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            33147..35164
                     /gene="hyfB"
                     /locus_tag="JQR05_000586"
                     /pseudo
     CDS             33147..35164
                     /gene="hyfB"
                     /locus_tag="JQR05_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            35175..36122
                     /gene="hyfC"
                     /locus_tag="JQR05_000587"
     CDS             35175..36122
                     /gene="hyfC"
                     /locus_tag="JQR05_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY5322826.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            36139..37578
                     /locus_tag="JQR05_000588"
     CDS             36139..37578
                     /locus_tag="JQR05_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY5322827.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLLGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            37590..38240
                     /gene="hyfE"
                     /locus_tag="JQR05_000589"
     CDS             37590..38240
                     /gene="hyfE"
                     /locus_tag="JQR05_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY5322828.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            38245..39825
                     /gene="hyfF"
                     /locus_tag="JQR05_000590"
     CDS             38245..39825
                     /gene="hyfF"
                     /locus_tag="JQR05_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY5322829.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMLHEV
                     ARGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGILKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            39815..41529
                     /gene="hyfG"
                     /locus_tag="JQR05_000591"
                     /pseudo
     CDS             39815..41529
                     /gene="hyfG"
                     /locus_tag="JQR05_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            41539..42090
                     /gene="hyfH"
                     /locus_tag="JQR05_000592"
     CDS             41539..42090
                     /gene="hyfH"
                     /locus_tag="JQR05_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY5322830.1"
                     /translation="MLKLLKTIMRAGTTTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTV
                     THKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCP
                     ECKQRATLINDDTDVPLVAKEQL"
     gene            42087..42845
                     /gene="hyfI"
                     /locus_tag="JQR05_000593"
     CDS             42087..42845
                     /gene="hyfI"
                     /locus_tag="JQR05_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY5322831.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            42838..43251
                     /gene="hyfJ"
                     /locus_tag="JQR05_000594"
     CDS             42838..43251
                     /gene="hyfJ"
                     /locus_tag="JQR05_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY5322832.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            43281..45293
                     /locus_tag="JQR05_000595"
     CDS             43281..45293
                     /locus_tag="JQR05_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY5322833.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEELLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCTAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            45315..46163
                     /locus_tag="JQR05_000596"
     CDS             45315..46163
                     /locus_tag="JQR05_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY5322834.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAILHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(46201..47262)
                     /locus_tag="JQR05_000597"
     CDS             complement(46201..47262)
                     /locus_tag="JQR05_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY5322835.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            47475..48938
                     /gene="bepA"
                     /locus_tag="JQR05_000598"
     CDS             47475..48938
                     /gene="bepA"
                     /locus_tag="JQR05_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY5322836.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            48959..49318
                     /gene="arsC"
                     /locus_tag="JQR05_000599"
     CDS             48959..49318
                     /gene="arsC"
                     /locus_tag="JQR05_000599"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY5322837.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(49451..50197)
                     /gene="hda"
                     /locus_tag="JQR05_000600"
     CDS             complement(49451..50197)
                     /gene="hda"
                     /locus_tag="JQR05_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY5322838.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(50247..51536)
                     /gene="uraA"
                     /locus_tag="JQR05_000601"
     CDS             complement(50247..51536)
                     /gene="uraA"
                     /locus_tag="JQR05_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY5322839.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(51622..52248)
                     /gene="upp"
                     /locus_tag="JQR05_000602"
     CDS             complement(51622..52248)
                     /gene="upp"
                     /locus_tag="JQR05_000602"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY5322840.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            52572..53609
                     /gene="purM"
                     /locus_tag="JQR05_000603"
     CDS             52572..53609
                     /gene="purM"
                     /locus_tag="JQR05_000603"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY5322841.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKCHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            53609..54247
                     /gene="purN"
                     /locus_tag="JQR05_000604"
     CDS             53609..54247
                     /gene="purN"
                     /locus_tag="JQR05_000604"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY5322842.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            54419..56485
                     /gene="ppk1"
                     /locus_tag="JQR05_000605"
     CDS             54419..56485
                     /gene="ppk1"
                     /locus_tag="JQR05_000605"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY5322843.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            56490..58031
                     /gene="ppx"
                     /locus_tag="JQR05_000606"
     CDS             56490..58031
                     /gene="ppx"
                     /locus_tag="JQR05_000606"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY5322844.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEDAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(58070..60313)
                     /gene="pdeF"
                     /locus_tag="JQR05_000607"
     CDS             complement(58070..60313)
                     /gene="pdeF"
                     /locus_tag="JQR05_000607"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY5322845.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRILGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(60495..60647)
                     /locus_tag="JQR05_000608"
     CDS             complement(60495..60647)
                     /locus_tag="JQR05_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322846.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            60665..60856
                     /locus_tag="JQR05_000609"
     CDS             60665..60856
                     /locus_tag="JQR05_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY5322847.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            61167..>61550
                     /locus_tag="JQR05_000610"
     CDS             61167..>61550
                     /locus_tag="JQR05_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY5322848.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYK"
BASE COUNT        14359 a        16430 c        15880 g        14881 t
ORIGIN      
        1 ggataagacg cggcaagcgt cgcatccggc atttattgcg ccgctgcgta caggccgaca
       61 atctgctcca ggctggcggt acgcgggtta ctgcgcagac aaatatcttc cagcgcggcc
      121 tgcgcccatg cgccgtaatg cgcagatgtc gcaccaacat cgcccagtcg tttaccaatc
      181 ccaacttcag caatcagctc acttaccgcg ttaatagcgt cactatcgtc ggatttttta
      241 gttcgcagcg cccgaccaat ctgactaaac cgatcacgac aaaccatccg gttaaactcc
      301 atcaccgttg gcagcaacat agcgttcgcg agaccgtgtg gaatatgcag cgccgcgccc
      361 ggctgatgcg ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc cattcccgcc
      421 atacatgaag ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct
      481 ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt
      541 gtagcgttca gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc
      601 gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgaggatcgc cacatccggc
      661 atcagcgagg catgggctaa cacctgcttg cgctcgctca ccgcgtcgat aatcaccgtt
      721 acattggtgg tttcagagcc ggttccggcg gtagttggaa tggcaatcag cggcaagcgc
      781 agttgcagaa cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc
      841 aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccgtca
      901 caacctgact cacgcaactg cgccacggct gcacacacgt cggtaatgca cggttcgccc
      961 accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg
     1021 gcggtcatcc ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca
     1081 cgcgtttgcg cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt
     1141 ggaacgctaa atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg
     1201 gtctgcaatt cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc
     1261 cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt
     1321 gcgaaacagt tctgccacgc ccggttgcat acaggagccg cccgccagcc ataaatcagt
     1381 aatgccctgc ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt
     1441 caccgcaggc caaatctctt caccgtgacc gcgcttgtac tgctccgcct cttccagcga
     1501 aatacggcga tttccggcga gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc
     1561 cgagtacatc actttgccct ttttcacgat ggcaatgccg gtagtgccac cgccgatatc
     1621 cactacaccg gcgttatcca gttgcagtag atccgcgact gcggttggct catccagcac
     1681 atggctcact tccagcccgg cagattccag cacgttgatg gaaatacgcg ggtccgtacc
     1741 tggaggaaat gaagtcgccg catggctaaa acgacgaccg aattgctgct cgagcgtgtc
     1801 gagatggcga cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc cgtcgcggac
     1861 cacatcggcc cagtcgaggc ataccgccac cggctgaccg tcgcggtcga caaccatcga
     1921 caccacatcg caggtgccca gatccacccc cagccacagc ggggattccg tcgcggcagg
     1981 cgtctggtta cacagcgttg ccgctgtttg cagacgtggg gtgagccatt gttcgtcgtg
     2041 cgccatcggt tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac
     2101 ggacaaacgt acgcgcgctg gttacccctt caccggttgg cgtggtgatg gtcatggtgg
     2161 tccagccttc cccgcccagt cccagcccgg caatgcacgg tccgttctta acgaaaatgc
     2221 tggtatcaat ggcattcgcc atctggttca tgttctcgat attgcgcgag tgcattgccg
     2281 ccgtgtggtg gcaaccgccc tccagtttca ccgccagcgc aatggcatcc gccacgttgg
     2341 cgacgcgcac gacgggcagc accggcatca tcagttcggt cacggcaaac ggatgttctg
     2401 cagtggtttc cacaaacagc aaacgcgttt cttgcggcac gttcaggccg attgccgccg
     2461 cgattttcgc cgcatcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt
     2521 cgatattttt cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg
     2581 cgtgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta
     2641 ccttttcgtc ggcacaaatg atgttgttat cgaaagacgc gcctttgacg atggactgag
     2701 cagcacgggc gaggtcagcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg
     2761 caatcagacg tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtca
     2821 ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat
     2881 ttgccacagt aaccagtaag ttttccggcc cacctgcggc aacaatcgcc tggttgagca
     2941 gcgtaatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt
     3001 tgcccgcggc aatcaggctg atggcgttgt taattacggt tgccgccggg ttagtggaag
     3061 gcgtcaccga agccaccacg ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc
     3121 cagtcagcac ttgcggagag aggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt
     3181 tttttgcaaa tttatcttca acgcgcccca tgccggtttc actgacggca agttccgcta
     3241 aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg
     3301 ccacgctttt taacccttgc tgggcgactt tggctgccgc aacggcgtca tccagggacg
     3361 cgaaaacgcc catctcatga acagcggcgg gcggcgtgtc actgctttgc attttcagca
     3421 gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg
     3481 gaaaattacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc acagatcgac
     3541 cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca gcaacaccca
     3601 ctccccggtt cccgcgccaa tattgtcgat ggcgacggca cattgcccgt caggattacc
     3661 ttgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac
     3721 ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt
     3781 caggtaaaag ctccccctac cctccgcaga aggtaaaatg aaaaaggaga gagcgtgacg
     3841 cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg
     3901 ggaacacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc
     3961 cgatacgctg tgccgctgcg gcaccggcat cggtcgccgc tttacaggct gcaacatcac
     4021 cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc
     4081 gcgcagcttt caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga
     4141 tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca
     4201 ttcaaccagt gtttgtaaac tgctttcgcg gttcatttct gtctgacgcg gcacagctgc
     4261 caccagcgcc agctcgataa tttcctgcac gctacaacca cgagagagat cgtgcatcgg
     4321 cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccgagtc gttgtgcgat
     4381 tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattgg ctttgccctg
     4441 tagcgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg
     4501 caactcgcca tcaaccacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg
     4561 gacgttggca acgcaggggt gacgggcgct accgttactg gaaaacgaca gcatcgccac
     4621 gcgcggctct tctccggtga gggcgcgcca ggtttcggca ctggcaagcg cgatatccgc
     4681 cagttgcgcc gccgtcggct gtggcaccac gctgcaatcg gcaaagccca acgccggacc
     4741 gctgtactgt ggcagcatca ggaaaatgga tgagagcgtt ttacagcctg gctgcaagcc
     4801 gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagaggttgc ccgcgataca
     4861 gacatccgct ttaccggcgc tgaccattgc tgcggcgaac atcagcggat cggtaagttt
     4921 ttccagcgca tccggcggcg ttttttcgcc cgcgtgggcc agccagcgat gagcaaattc
     4981 ttcccgcatt gcgaggttgc catgcggatc aatcacctgt agcccgtcca tcgccacgcc
     5041 gtgactgagt gcaaactgac gaagttcaaa cggattagcg accagaatgg gcgttgccag
     5101 accttgttga tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg catccggaaa
     5161 aaccactctg gcgggcgctc gcaacgccag ttcaatttgc agccacactg ccagcgcaat
     5221 ggtgctgtcg agaaccgcgc gaaaacccag acgcggatcg cttttggcga ccattttttc
     5281 cgccgacagg tgagtcagcg tatccggctt ttttgccacc agctggcggc acagttcgca
     5341 gcacgcctgc ggatgttcat cgctactagt cagcccatga acgggctgcg gctgctgctg
     5401 ttcatcgtca acaaacaggc ggccctgctc gtcaataaac ttgatgcgca gatggcggct
     5461 ttccagcaac tcccgggcag agggcgtcag gcgactgtcc gcagggagat ggatctctgc
     5521 gccttcgctg agcgtatggt tcgctcttag ccatgcttcg gtgatgaaat ctttcatagg
     5581 gattgccagt tagccggcca ggcgacatac aggaatttca cgcttgatgt cgtaccaaat
     5641 tcgatgctgg agcctttcgg gataaacatc acgtcgcctg ctttggcgat catggtttcg
     5701 ccttcgtggc gaacatgcag ctccccttcc agcaccatgt cgatttcgtc gtagttcagg
     5761 gtccacggga agaatgcgtt ctcccactgc ataaaaccgg cggccatgct gctgccgtcg
     5821 tctccggtca caagatcagt caagcccacg caatgcggct ctgcgccgtc gaaacgacca
     5881 aacttgacgc tgctgccatc aatcactttg atgccgcctt tgccggtcac cgatttgaag
     5941 ctcggctgca ttgcgccctg ctccagcgac tgtttttcct tcatcacttt ttccatcagc
     6001 tgtgcgacca ggctttcggt aaactggcct tccggcagct gggcaatgat ggtttcgcgg
     6061 atgcgctggc tttcggtttt gtcggctggc acgctggcgg gcacggacgc cgttaccggg
     6121 atcgattcat cacattcggt gatggtaaaa cccagtagat ccgccacttc acgggcttcc
     6181 ggggtaataa tgctggcgcg cagaaccacg gacattgcct gttcgccgcg cgcgtgtgct
     6241 tcacgaatat cattcgctgt gatgagtttt ttcacctgct tcctcctgtt tggtgagaga
     6301 tgccagataa tccaccagtt gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat
     6361 cggctcttcg aaacccgtct caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg
     6421 catgtccgtt ttactgatga cggcgatttg ccgtttgctg acgccaatat ccagcaaccc
     6481 ggcaggcaac cgactttccg ggtcatttgc gccatggaca tagatcagca tgtctacatc
     6541 ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg ctaaaatatt cacccggcgt
     6601 atcaatatcg cccttatcat taaattccac ggcctgtgtt tttctggcga gggtataatt
     6661 cccctgcaac gcattaaata acgttgtttt tcctgccccg acgctaccca caaacgcaat
     6721 tcgtttcatg aatgcctcgt attaactttt ggtcatttca caaagcgtat aatttaataa
     6781 gcgacctaaa ccgctgacgg tttgtgataa cgcttcttct actgcgccca ccgagccata
     6841 aatcaccaat gcaccgctga agcggtcgag aaaaccgata tgcacatcag cggctttcag
     6901 cgctaaatca ccggcgatca tcgccgtttc gccgggagtt agcgtcataa tgccaattgc
     6961 gcccgcatcc ggaacgccga ttttcttcgc aagttcctca ccgggatgcg cgatgagatg
     7021 cgccagtgtg acctgtttgc cgggcacaaa ttcctgaatg atgcgttctt tatccatgag
     7081 tcgccgcctg tgaaaaactt tcgtgtatcg tgacaatttt tcgtcagagt aaataacaaa
     7141 attcggcaag cgcgtttaaa aggtgaggta gatcactaag aatggagaga tatttactgt
     7201 tatgaataaa ctttcatatg cgacgcatga atataaataa aataatatcg agatatttta
     7261 tatactgatt tttttattat tcgcaaactc agtaataagc tttgctaata tatttcagga
     7321 aaaccgctta ccctgaatat tcagggtaag cgtgagagtt aagaatgaat tacagcggtt
     7381 gggtttgcgc ttcaaccacg gccagcgcca ccatattgac gatacgacgc accgatgcga
     7441 tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac
     7501 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
     7561 tcaccagaat attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg
     7621 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagatct ggcgcacgat
     7681 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
     7741 agttggagtg cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg
     7801 ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat
     7861 cggcaataaa ggtgttacca ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa
     7921 cgccatcgcg ataaccaaag acatttttca ccacgctaaa atgttcatga taatcgccca
     7981 ccgtaccgca gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg
     8041 tcgggttact gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga
     8101 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgattt
     8161 caaaatcaac gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
     8221 tcggacgacc gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat
     8281 gcagaacgcg cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct
     8341 gggagaaaat cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga
     8401 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgcttttg
     8461 cgaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     8521 ccggaccaaa gctcagatcc tgaacgccat acgctgaagc caccacttca ctctgttctg
     8581 catgggccag ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg
     8641 ttgcgccaac gtccagcgcg ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct
     8701 ggttcgggta gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca
     8761 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggt gcccgagcca
     8821 ttttcttcac catttcctgg gtcagcactt tcgggccgga acagcccagg aaaatatccg
     8881 caccttcaat cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt
     8941 tggtttccgc catatttggc tcacggccct gatagataac gccttttgaa tcgcaaacca
     9001 cgatgttatg tttttgcagc cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     9061 cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc
     9121 cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg
     9181 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     9241 cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc gacgacttca ataaatttgt
     9301 ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaata
     9361 gaacgccctt gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca
     9421 gcaccgccgt accgttagag atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg
     9481 cttttagcgg gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     9541 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     9601 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     9661 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     9721 acattattac cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata
     9781 taatgttttg gtaataatcc tataacactg atgttacctg cttaatccag caataccatg
     9841 cctgtctgct atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc
     9901 actcatctaa cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa
     9961 acagttcacc actgtcgtgg cagacagcgg cgatattgag tccattcgcc attatcatcc
    10021 ccaggatgcc accaccaatc cttcgctgtt actcaaggct gccggattat cacaatatga
    10081 gcatttaata gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca
    10141 ggtggtcgca gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt
    10201 acccggttgc gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat
    10261 tgagaaggcg cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat
    10321 tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga
    10381 aggtattaac tgcaacctga cgctgctgtt ttcttttgcc caggcgcggg cctgtgcgga
    10441 agcaggcgtt tttctgattt cgccgtttgt agggcgtatt tatgactggt atcaggcacg
    10501 caagccgatg gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat
    10561 ctacgactac tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg
    10621 caccgaacaa atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgtt
    10681 gaaggagctg caggaaaaag tttcgccagt ggtacgtaaa ttaatcccgc cttctcagac
    10741 gttcccacgc ccagcaccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc
    10801 gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact
    10861 ggaagatctt cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag
    10921 accttgccaa tgcgattcgc gcactcagta tggatgcggt acaaaaagcc aattctggtc
    10981 atcccggcgc gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta
    11041 aacataatcc taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc
    11101 acgcgtcgat gctgctctac agtttgctac atctgaccgg ttacgacctg ccgctggaag
    11161 aactgaagaa cttccgtcag ttgcattcga aaaccccagg ccacccggag attggctata
    11221 cgccaggcgt tgaaaccacc accggcccgc ttggacaagg tttggcgaac gccgtcgggc
    11281 tggcgatagc agagcgtaca ctggcggcgc agtttaacca gccagaccat gagatcgtcg
    11341 atcacttcac ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag
    11401 tctgttcgct ggcaggcacg ctgggactgg gcaagctgat tggtttttac gatcacaacg
    11461 gtatttccat cgacggtgaa acagaaggct ggtttaccga cgatacggca aaacgttttg
    11521 aagcctatca ctggcatgtg atccatgaaa tcgacggtca caatccgcag gcggtgaagg
    11581 aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg
    11641 tgattggctt tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg
    11701 gggaagaaga agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga
    11761 tccctaaaga gatttatcac gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga
    11821 gctggaatga gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagttta
    11881 cccgacggat gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca
    11941 atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta
    12001 acgcttacgg gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca
    12061 acctgaccat ctggaaaggt tctgtttcgc tgaaggaaga tccggcgggc aactacattc
    12121 actacggggt gcgtgaattt ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg
    12181 gctttgtgcc gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc
    12241 ggatggcggc actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc
    12301 tgggcgaaga tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc
    12361 caaatttcag cacctggcga ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg
    12421 cggttgagcg ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg
    12481 tggaacgtac gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca
    12541 gcggcggtaa gccagatatt attctgattg ccaccggttc agagatggaa attaccctgc
    12601 aagcggcaga gaaattagca ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga
    12661 ccgatatttt cgacgcccaa gatgaagaat atcgggagtc ggtgttgcct tctaacgttg
    12721 cggctcgcgt ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga
    12781 aaggggcaat tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc
    12841 cgttctttgg ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga
    12901 aaggtgcctg atggtgatcg ccggatgctg tatgttcccc tcaccctaac cctctcccca
    12961 gaagggcgag gggacggtcc gtgcgctttt tagggccgca tctgcgattt tgtttaacgg
    13021 gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg
    13081 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
    13141 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
    13201 gcgttcatca gttccagttc attcgtctcc tgaccgttgt tgaacggcaa acgcaaccga
    13261 accgggcgcg aggctagcgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
    13321 taggcccctg cctcacagct aatcatcatc agtgctttat catcggtcag cgcagtgaca
    13381 ttcacctcac gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
    13441 taatccagca aatcgttacg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
    13501 gcgacctctt tcagcgcagg cgcgggcggt acgctgagag gcggttcgtc ccctttcttg
    13561 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct
    13621 tttaagccgc tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc
    13681 tgaattatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
    13741 gaaatccgcc acttgtcacc acttaatgcc aacggctcac catctaatag cagccgtggc
    13801 gctatctccc cttcagacgc atccggcgac ttcaacccac cgcgctcaat acgtaaaacg
    13861 gcatcggtat gcgccccagc gctgcggctc agggtcatca ccagtccatt atgatcgccc
    13921 gtattgcgcg ccacacaaaa attttgatta ttacaggtga cctgccagtc ggaaaacgcc
    13981 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
    14041 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
    14101 atcttcgttg cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac
    14161 aacgaaatat tattgttatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca
    14221 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
    14281 acggcagctc gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct
    14341 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca
    14401 tatacagttc aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt
    14461 ctttacgaat gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
    14521 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
    14581 cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt
    14641 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca
    14701 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
    14761 cgactccaca gcgcgaaatg aacaattata ttacgctgtg aagtcgggtt tgtctgcgca
    14821 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
    14881 tgacgtcttt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg
    14941 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
    15001 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg
    15061 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt
    15121 acaggtcgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatt
    15181 ccgcaaacgt gaccttgctc attcagctca agcgccaccg gctggacgtt aaattcgaag
    15241 ttggccccct cttcgcgggc gttcttcacc tctttcttcg agcctggcat gttagcttca
    15301 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
    15361 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac
    15421 ggctcttccg gtagctcttc gagcctcatc acctgtttag tgttggcaat gaggaacggc
    15481 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa
    15541 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcgaatccaa agagacatct
    15601 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
    15661 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagcgag caagccaccg
    15721 atttctggat ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca
    15781 caggccagcc ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca
    15841 tgacttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaatg
    15901 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
    15961 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
    16021 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc
    16081 agcgggcagg tccattcaca aacgctgtgc tcgccgaact taaggcagcg cgaggcttcc
    16141 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
    16201 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
    16261 ctcattaccg gcatttcttg tgcagcggta ctggcatgcc acggttgatg ctcctgacgt
    16321 gcggtgcgca agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
    16381 agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg
    16441 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
    16501 atcgtaccaa acggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
    16561 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
    16621 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gctgatgttt gataaccgta
    16681 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
    16741 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
    16801 ttcataacga ccttcttttt tggttgtaaa aaccttattc ttatatgagt gttgttatta
    16861 cccgacttac aggggaaccg gcaatgttca tgtgcccaga ataagtaact atttcgctat
    16921 aaactgtggc agatcaaata atcccatcac tgactaaatt gcgtttcagc gaactggaac
    16981 attaatgatt ttttgtggag aagacgcgtg tgattgttaa acgacccgtc tcggccagtc
    17041 tggcccgggc ctttttttac attgtgctgc tgtcgattct ttccacgggt atcgctctgc
    17101 taactctggc gagcagtttg cgcgacgctg aggctatcaa tattgccgga tcgctgcgta
    17161 tgcagagtta ccgcctgggc tacgacttgc aaagtggcag tccacaactc aatgcacatc
    17221 gccagttgtt tcagcaggca ctgcattcac cggtattaac caacctcaac gtctggtatg
    17281 tgccagaagc agtaaaaact cgctatgcgc atctgaatgc caactggctg gagatgaata
    17341 atcggctcag caagggcgat tttccgtggt atcaggccaa tattaataat tatgttaatc
    17401 agatagacct gttcgtgctg gctttacagc actatgctga acgcaaaatg ctgctggtgg
    17461 tggcgatttc cctggctggc ggtatcggta ttttcacgct ggtctttttt actttgcgcc
    17521 gcatacgcca tcaggtggtt gccccgctga atcagctggt taccgccagt cagcgtattg
    17581 aacacgggca gttcgactcg ccgccgctgg ataccagtct gccgaatgag cttggtctgc
    17641 ttgcaaaaac ctttaaccaa atgtcgagcg agctgcataa attgtacctt tcgctggagg
    17701 cgtcagtaga agaaaagacc cgcgatctcc acgaggccaa gcgtcgtctg gaggtgttgt
    17761 atcagtgttc ccaggcgctg aacaccagcc agattgatgt gcattgtttc cgccatattt
    17821 tgcagattgt tcgcgacaat gaagcagctg aatatctgga gctgaacgtt ggggataact
    17881 ggcggattag cgaagggaag ccaaaccctg agctgccaat gcagatttta ccggtgacaa
    17941 tgcaagagac ggtttacggc gaactgcact ggcaaaatag tcacgtttca tcatcagaac
    18001 cgctgcttaa cagtgtttca tcgatgctgg gacgcggttt gtactttaat caggcgcaga
    18061 agcattttca gcagttattg ttgatggaag aacgtgcgac catcgcccgc gaattgcacg
    18121 actcgctggc tcaggtgctt tcttacttac gtatccagtt gacgttactg aagcgttcga
    18181 taccggaaga taacgccact gcacaaagta tcatggccga tttttcccag gcgttgaatg
    18241 atgcttatcg gcagttacgc gagctgttga ctactttccg cctgacgctg cagcaggcgg
    18301 atctcccctc cgcgttgagg gaaatgctgg atacgttaca aaatcaaacc agcgctaaac
    18361 tgaccctcga ctgccgtctg ccaaccctgg cactggatgc gcaaatgcag gtgcatttgt
    18421 tgcaaattat tcgcgaagcg gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg
    18481 ccatcagttg cgtcaccgcg ccggacggca atcacacggt ttatatccgt gataacggga
    18541 ttggtatcgg tgaaccgaaa gaacccgaag gtcattatgg actgaatatc atgcgcgaac
    18601 gcgcggaacg gctgggcggg acgctgactt tttcacaacc ttccggcggc ggcacgttag
    18661 tgagtattag ctttcgctcc gcagaggggg aggggaaggg gaacacgccg gctgaggtgt
    18721 aaataattac cctcacaccg gccctctccc tttaaaggcg agagggaaaa acgtgcccat
    18781 attgacctgt aagcgagtgt gtcagcaatc tcatgataat ttacattaac tccttttttt
    18841 ctccacgatt ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacaatacg
    18901 cagaaacacg aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct
    18961 gggtgctggc aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg
    19021 aacaataccc cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat
    19081 cggcccagac gctggaaaac accgtgaccc aggttatcga gcaaaatatg accggcctcg
    19141 ataatctgat gtatatgtca tctcagagca gtggcaccgg tcaggcatct gtcactttaa
    19201 gttttaaagc aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat
    19261 cagccatgcg aaagttaccg caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg
    19321 gcgataccaa cattctgacc attgccttcg tctctaccga tggctcgatg gataaacagg
    19381 atattgctga ttatgttgcc agtaatattc aggacccgtt aagccgcgtg aatggcgtcg
    19441 gggatatcga tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac
    19501 tcaacagttt ccagatgacg gctaaagatg tcactgatgc cattgagtca cagaacgcgc
    19561 agattgcggt tgggcaactt ggtggtacac cttccgtcga taagcaggcg ctcaacgcca
    19621 ccattaacgc ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg
    19681 tgaatcagga cggctcagag gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg
    19741 agaaatacga ttatcttagc cgcttcaatg gtaagccagc ctccgggttg ggggtaaaac
    19801 tggcctccgg cgcaaacgaa atggcgacag cggagctggt gctcaatcgt ctcgacgagc
    19861 tggcgcagta tttcccgcac ggactggaat acaaggtggc gtatgaaacc acctcgtttg
    19921 ttaaagcctc cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc
    19981 tcgttatgta tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc
    20041 cggtggtgtt gatgggaacc ttctccgtac tttacgcctt cggttacagc gtcaacacct
    20101 taaccatgtt cgcgatggtg ctggcgatcg gtctgctggt ggatgacgcc atcgtggtgg
    20161 tggaaaacgt cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacacgta
    20221 aatcgatggg gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat
    20281 ttgtaccaat ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta
    20341 ccattgttgc ggcgatggtg ctgtcagtac tggtagcgat gatcctcact ccggctctgt
    20401 gtgccacgct acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggtttttttg
    20461 cctggtttaa ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa
    20521 ttctccaccg tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt
    20581 tcctgttcct gcgtttgccg acgtcgttct taccgctgga agaccggggc atgtttacta
    20641 cctcggtaca attgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa
    20701 tcgagaaata ttacttcacc catgaaaaag acaacatcat gtcggtgttt gccaccgttg
    20761 gttctggccc tgggggtaac gggcaaaacg tggcgagaat gtttatccgc ctgaaagact
    20821 ggagcgaacg cgacagtaag accggcacct cgtttgccat tatcgagcgt gcaacgaagg
    20881 cgtttaacca aattaaagaa gctcgcgtta tcgccagcag cccaccagca attagcggtc
    20941 ttggtagttc tgcaggtttt gatatggagt tgcaggacca cgctggagcg ggtcacgatg
    21001 cgctgatggc agcacgtaat cagttgctgg cgctggcggc ggaaaacccg gagctaaccc
    21061 gtgtgcgcca taacggcctc gatgacagtc cgcagttgca gattgatatc gaccagcgta
    21121 aagctcaggc gctgggcgtt gatatcgacg atattaacga cacactgcaa accgcctggg
    21181 gttcgagcta tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg
    21241 cagctgcgcc gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag
    21301 atggcggcat ggtgcccttc tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc
    21361 gtctggaacg ctataacggt tattctgcgg ttgagattgt tggggaagcc gcaccggggg
    21421 tcagtaccgg tacggcgatg gatattatgg aatcgttagt gaagcagctg ccaaacggct
    21481 ttggtctgga gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg
    21541 cgctgtacgc catttccttg ctggtggtat tcctgtgtct ggcggcattg tatgaaagct
    21601 ggtcggtacc gttctcggta atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg
    21661 caacctggat gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca
    21721 ttggtttatc ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa
    21781 aaggccacga cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc
    21841 tgatgacctc gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgccg
    21901 gttccggtgg tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcagctacta
    21961 ttctggctat ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga
    22021 agccgcgccc ggaataagca ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact
    22081 ttccgattaa gaacctgctc agcgggttct tgctgtttat actttgtctc aggaattact
    22141 tacgaagcat aacttcgata aagtctttcc agttccccag ttcacgttca atcataacaa
    22201 cctctcttat aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact
    22261 cgattgctgc gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcgg
    22321 tgataatttg tatgtgatac acagcaacat ttcgagatat tcatacggca tctaatactg
    22381 atttaattct ggttaaaata cagacagata acaagatgaa tattcttaat gtttacgtta
    22441 aaaatgttta atattattta atagttgtta atttgaatac ttcgataatg ttatattttc
    22501 tgataatcat ttgcaggcaa aatgttttca cccttaaatg agtatttatt ctcataaatc
    22561 gaaaaaggat tcattatggt tacactttac ggcatcaaaa attgtgacac cattaaaaag
    22621 gctcgccgtt ggctggaagc caataacatc gactatcgtt ttcatgatta ccgcgtcgat
    22681 gggctggaca gcgaattatt gaacggtttt atcaacgaat taggctggga agcgttactc
    22741 aacacccgtg gtacaacctg gcgtaaactg gacgaaacta cccgcaataa aatcaccgat
    22801 gcggcctctg cggcggcatt aatgactgaa atgcctgcaa ttatcaaacg tccattgctc
    22861 tgcgcgcctg gtaagcctat gctgctgggt ttcagtgatt ccagttatca gcaatttttc
    22921 catgaggtgt agtctatgtc gtgcccggtt attgagctga cacaacagct tattcgccgc
    22981 ccttccctga gtcctgatga cgcaggatgt caggctttgt tgattgaacg tttgcaggcg
    23041 atcggtttta ccgttgaacg catggatttt gccgatacgc agaatttttg ggcatggcgt
    23101 gggcaaggtg aaacgttagc ctttgccggg cataccgacg tggtgccgcc tggcgacgcc
    23161 gatcgttgga tcaatccgcc gtttgaaccc accattcgtg acggcatgtt attcgggcgc
    23221 ggtgcggcag atatgaaagg ctcgctggcg gcgatggtgg tagcagcaga acgttttgtc
    23281 gcacaacatc ccaaccatac ggggcgactg gcatttctga tcacttctga tgaagaagcc
    23341 agtgcccaca acggtacggt aaaagtcgtc gaggcgttaa tggcacgtaa tgagcgtctc
    23401 gattactgcc tggtcggcga accgtcgagt atcgaagtgg taggtgatgt ggtgaaaaat
    23461 ggtcgtcgcg gatcgttaac ctgcaacctc accattcatg gcgttcaggg gcatgttgcc
    23521 tacccacatc tggctgacaa tccggtacat cgcgcagcac ctttccttaa tgaattagtg
    23581 gctattgagt gggatcaggg caatgaattc ttcccggcga ccagtatgca gattgccaat
    23641 attcaggcgg gaacgggcag taacaatgtt attccgggtg aactgtttgt gcagtttaac
    23701 ttccgcttca gcaccgaact gactgatgag atgatcaaag cgcaggtact tgccctgctt
    23761 gaaaaacatc aactgcgcta tacggtggat tggtggcttt ccgggcagcc atttttgacc
    23821 gcgcgcggta aactggtgga tgcggtcgtt aacgcggttg agcactataa tgaaattaaa
    23881 ccgcagctac tgaccacagg cggaacgtcc gacgggcgct ttattgctcg catgggggcg
    23941 caggtggtgg aactcgggcc ggtcaatgcc actattcata aaattaatga atgtgtgaac
    24001 gctgccgacc tgcagctact tgcccgtatg tatcaacgta tcatggaaca gctcgtcgcg
    24061 tgatgagtgg ttctgcaaga ggaaataagc atggactggc tggctaaata ttggtggatt
    24121 ctggtgattg tctttttggt aggcgtcctg ctgaacgtga ttaaagatct caagcgcgtc
    24181 gaccataaga aatttctcgc caacaagccg gagcttcccc cgcatcgtga tttcaacgat
    24241 aagtgggacg atgacgacga ctggccgaaa aaggatcaac cgaagaagta agctgagtgc
    24301 tcaaccgtag gccggataag gcgttaacgc cgcatccggc aatggtgaac gatgcctgat
    24361 gcgacgctca cgcgtcttat caggccaggt aatctgtctt ttacatcatt tcaatcacat
    24421 catcatcgcc aggcttaccg ccacttagcg cttcgtcgaa ataatgtttc ggaatggtat
    24481 aacgcaagtg atccagggca aattgcatac tgcgattgtc gatcgcatgg cctaaatcct
    24541 ccacgatatc cagcgtcacg tcaccaccgg cactgattaa cgcttcctgc gccgcaactg
    24601 catgcgccag atcgataacc ggatcttcac caccatgaat caagtgaatt gtcgtggctg
    24661 tagacgcggt ctccggcagg ctcgcatagc gtccgttaaa ggcaatgacg cgcgaagcaa
    24721 ggcccggttc ggctttaatg ctctccagcg ccataatcgc gccttgtgag aaaccaatta
    24781 gcgccgtggc gttagcccct actccgctct gtttctgcca gtagcgtacc gtttcaataa
    24841 acgtcggcat gatggcatcc acacgcgcct ggcgattatc ttccgtaata ccctgcactg
    24901 aaaaccactg acgccccgcc ggattaccgc tcggctccgc gccaccgaca ctcaccacca
    24961 gcgcatcagg aaacagcggt gcaaaccagc tgcctatttc ccccattgcc accgggttat
    25021 ccccgacacc gtggaaaagc agtaacaact gttgtgcagg tttatccggg ctttgaacaa
    25081 caaaatggtc atgtttcatg gcgaactcct taactgatgt cattaatttt acgccgctga
    25141 ctcacaatga ccatgggaga aaattgaagg agtcagtgaa aaaattgcca ttgcgttatg
    25201 cgatcgcgca gacgctcagt gcgaacctca ttaagtgcga acagcgcctg cgcggcctct
    25261 tcccgctggc gaaccagtaa catcttgcgg cctgaaagtt taagtgtggt acataactgc
    25321 gcgtcactgg cgtttttctg taaacgccca cgcagcgccg gtaatgccag ttcactggtt
    25381 tgtagcagac gcaataagca acctaacgat gttaatagcg gacgatgagc gaaagcaaaa
    25441 ccggccagtt caagccagtc gtcgtcagaa aggatggcat cgtttagtgg atcaacagga
    25501 agcatttcgc cattccactg cgcgagagct tgcgcatcgc gacgtaaacg gtaatgctca
    25561 cgttcagcca gctgtttacc cgcatcactc atcggtaaca gcgccatcgc cgtatagcaa
    25621 ccgctgctgg cttcccgatg attacccatc cgcaccagca caaaaccgca gcgttgccag
    25681 aaacgccata actccccggt gtaaccaaaa ctcaccgaaa gatagtcgag gtcatgcgta
    25741 tattgcaaag ctccagcaat aagttgccgc cctgtgcctt cccgctgacg agccggatga
    25801 actgctatcc ggctaacccg ccttccgcgc aatgtcgccg ccagtggatt gctgccgtgc
    25861 gccgccagcg actgggccac cagattaccc cgcgggcgac gaaaacctgc ccataccgcc
    25921 tgactgagtt cttgagataa tccgccctca tccaccagcc acaacgcccc ggcaatctcg
    25981 ttttcgccag ccgcctgtaa aaaatgttgc cctggtgcat ccatcatccg gcgtaaatcc
    26041 agcggcgagg tccggtagtg cgcaccagac aataactgat aaacctttaa cggcgtttct
    26101 ggctcgcttc gccataacgt ttgttcaaat gcggaaatga cgatattgcc ttgtggtgta
    26161 tgggtgaagt tttcatcgtt aaaaaccagt gcctcactaa ccattttttc cagcgggcat
    26221 ccctgtgccc agcggatcgg ctgttgcagt tcaaaacggt gtaaatgcgg aaagcgagcg
    26281 caaaatttca gcaaaaaacc acgtccggta ccttcgtagc cctgcaccgt agtggttaac
    26341 aacgttcgag gaaaacgcga taccagttga tgcaacaacg gcgcaggtat ggctgcggct
    26401 tcgtcgacca ccagccagtc ggcttgctca tcgctggcta acaaggcatc cggtgcaata
    26461 aagcgaaact tctcgcccgc aaattgtgcc agtacatccg ttgccgcttt tgcgggcgcg
    26521 gtgacaatcg cactgcccgc aatacgagaa atgagttgcc ctgccaacgc cgacttaccg
    26581 cgcccacgcg cagccgttac cgctgccacg cccggcggca tggtcagtag ctgctgtaag
    26641 agttgctgtt gttctggttg tggtgcgccg gtcgcggggt gccagtcagt acggggagta
    26701 aaatgcgcca acgagaacgg ctggttttgc cgccagagga tagcgtcgtt atccgccgta
    26761 agtacgcgtt tgagatgctg gacaaaatgc ggcgtcgcaa tagggtcagg gcaatcactc
    26821 cagcgcagcg agtcggcatc aggttggttt tcccactctt cccatacagg gagtaacaaa
    26881 accagccagc ttcccgcttt caacgttccg ctaagtgccg caaaggcagc ggcatcaaag
    26941 ccgtggcggg cgtcgaatac cgcatgccgg aactcgcgcc caagtaaagt ttgtagcgcc
    27001 gagggtgaac agtggttttc agcatctagc tgcggcgaaa tccacagcca gtcgccaggt
    27061 aaggcatcac gcaacttaag agcatgatca aaacaccaac cctcttcccc gctcaacacc
    27121 agcaagcggc agatcccttc acgtttcatt tgtgctgtta atgtgtaaag cgcagtcagt
    27181 tcagccatcc ctgccccgaa aattaaatgc ttttaccaaa agtattgcat tgtgccggat
    27241 cgccgctgtc gaaaccacgt ttaaaccagc tgtagcgttg ctgagaagtg ccatgtgtga
    27301 aactgtctgg tactactcgc ccctgacttt gctgttgtaa acggtcatcg ccgatggcct
    27361 gcgccgcatt cagcgcctct tccagatcgc cagtttccag aacgccttgc tgctgcatac
    27421 tatgccccca gacaccggca aaacagtcgg cctggagttc catacgcaca gataagtggt
    27481 ttacttccgc ctgcgtcgcg ttttgttgca gttgacgaac tttcggctcg atgcctaaca
    27541 gtttctgcac atgatgaccg acttcatggg cgataacgta cccctgggca aaatcgccat
    27601 ccgcgccaag tttgtctttc atgtcatcat agaaggagag atcgatataa actgtgccat
    27661 ccgccgggca atagaacggc cccataatgg actggcccgc cccgcagccg gtgcgcgtca
    27721 ttccacggta catgaccagc ttcggttgct gataggtctt acccatcttt tcgaactgtt
    27781 gtccccaggt gtcttccgtg gttgccagaa tcaccgaggt gaattttgcg gcttcgtctt
    27841 cattagggct aattgaccgc gttgattgtt gttgagaaac cggctgcccg gtcatcaacc
    27901 cggttaaatc aacgccatag tagcctgcaa ccagcacgac tatcagtaaa atcagcccgc
    27961 ctttaccgct cggcagacgg aaaccgggac cgcccattga cggaccgcca gagctgttgc
    28021 gcctgtcttc aacattgtca ctttcacgtc gcccttgcca acgcatagat acctcaacaa
    28081 tatattcatt atagaaatga tcgtaggtgg ttcagaggaa gattaccaca ggaaagcggg
    28141 atgaaatggc ggaggaatga gcgttcaatg catatattcc gagagcggtt gtcttgccgt
    28201 gccagctgca cggcaagatg atgaacagaa aaatcagtcc agctgtacac ccaggcggcg
    28261 ggcgacggct tcataggctt cgatcaggcc accgaggctc tggcggaaac ggtctttgtc
    28321 cattttctcc agtgtttctt tgtcccacag gcggctgccg tccggggaga actcatcgcc
    28381 cagtaccact tcacctttgt acagaccaaa ctccagcttg aagtcgacca gaatcagacc
    28441 agcatcatcg aacagttttt tcagcacgtc gttcgctttg taggtcagct ctttcatacg
    28501 cgccaggttc tctttgctca cccagccaaa ggtttcgcag taagattcgt tgaccatcgg
    28561 atcgtgcatg gcatcgtttt tcaggaacag atcgaacagc ggcgggttca gctcaatacc
    28621 ttcttcgatt ccaagacgtt tcaccagaga gccagcagca cggttacgca cgacacactc
    28681 aaccggcacc atatccagct ttttcaccag acattcggta tcggagagca gacgctccat
    28741 ttgagtcggg atacccgctt cagccagttt gctcataatg aagtagttga acttgttgtt
    28801 caccatacct ttgcgatcaa actgctcaat gcgcgcgcca tcccctgctg acgtatcatt
    28861 gcggaattcg agcaccaaca ggtccgggtt ttccgtgctg tatacggttt tcgctttacc
    28921 acgatacaac tcagcttgct tttgcatctt tatcactcct gggtgtgaat taacgtttta
    28981 aaatcttttg ctgtctggtg tgccggatgt tttgtcggat gcggcgtgaa cgccttatcc
    29041 gaactacgtc cgacaaacaa atttcgtgcg aattaccgct aaaatcgcgt tttcctgccg
    29101 acgcacacgt ttgcgtatta tatcagaaaa aagggccgga tgattccagc cctgtatttt
    29161 tacttgctaa acgcagcctg gaagacagct accagcgcgt cgttctgact ctgagtcaga
    29221 gtatgacctt tcggatcgat gaactgtagg ctgctgcggt tatctaaatc gccaacctgc
    29281 agtttatagt caccggatgc caggcctgga tcgctcgcgc ccagttcctg ccagtcgctg
    29341 tcagacagcg gcttataagt tacggccatg ttgccctgcg aacgggtgct gtcggtcact
    29401 ttcatgccca ctttttccag cgccgctggc agacgttgcc aaaccacatt gaacggcccg
    29461 cgtacgacca gcattggtaa accggtgtca tcagctgcac tttgtacgtc catagtggtg
    29521 gaggcacgat tttgcgcagc gttcgcggcg tcagtggcag atttatccag accggcggaa
    29581 ataacgttca tcatctccgt gctgtaacgc tgcatggaag ccgcgtctgc aaccggtttg
    29641 cccgcctgtt ccaggttcag cagtttaacc gtaaccgcct gctgataacc ctgcggctta
    29701 acagagattt gataacgacc acgatactgc tcgtcttcgt ccagacggtt ccattgtacc
    29761 caatcggtgg tcagtgtctg accagcatca tcacgttggg tgatggtgta gtttttcgcc
    29821 tgcagcacgc taaccacctg cggccacaga gtattgccac gaccattttc caccagcaat
    29881 gaagcggtat cgcccgtgaa ctgggtacgc gcgccagaaa ccagtgccag cggctgggct
    29941 ggtggacgaa tgtccagcgc cttaccgaca gcaccactac cgttggtcac cgggattgca
    30001 taatcaccgg aggtcaccgg caaaatcatt ccagccgggg catgaagctc cgcaagcggt
    30061 gccgcttcca ggtaggcttc atcaccactg acctgacgct tatagcgtga gtcagaacta
    30121 caggcagcga gtaataaaac aagcgaaaca cccgcaacct ttgccaggcg cgacttttga
    30181 acagagtaag ccatcaaatc tccctaaact ttacagcaaa ccggcatgct taagcgccgc
    30241 tctgaccgtc tcacgaccac tgtcggtgat tggtgtcatt ggcaggcgca gcgtatcggt
    30301 cgccacaaga cccagttcct tacatgccca tttcaccggg attggattgg gttcgacaaa
    30361 tagtttgttg tgtaatggca tcagacgctg attaataacg cgtgcctcgg caaaatgccc
    30421 ttctgctgcc agtttgcaca tctgggccat atcacgcgct gcgacgttag ccgtaacgga
    30481 aataacccca tgaccgccca attgcatgaa gtccagcgcg ctcgcatcat cgccgctcag
    30541 cagaacaaaa tcatctgaaa ccagctcttt gatctggttt acacgcgtta agttccctgt
    30601 tgcctctttg attccgataa tattttttac tttcgccaga cggcccaccg tttccgggag
    30661 cagatcgcag ccagtacggg acggcacatt atacagaatt tgcggcaggt cagtatgctc
    30721 agcgatggct ttgaaatgct gatacaaacc ttcttgcgac ggacgattgt agtaaggagt
    30781 taccgtcagg cagccgacga taccactgac attgaagcgc tgcgtcaggc taatggcttc
    30841 cgcagtagcg ttagcgccgg tcccggcaat taccggaatg cgcccatcag ccagatccag
    30901 cgtcatcatc accacatcag catgttcgtc atgatttaag gtagcggact cgccagtggt
    30961 gccaacagaa acgatcgccg aagtaccgct ggcgacatga taatcaatca gttttttcaa
    31021 gctagcccga cagacattac ctttttcatc catcggagta acaatcgcga caatacttcc
    31081 cgtgaacatg ggccatcctc tgtgcaaaca agtgtctcaa tggtacgttt ggtatggcat
    31141 taaaagcaag cagacagaac cgttctgatt gttgtatgca tgattttttt atgctttcct
    31201 taagaacaac tcacccctta aaggaataac cagtttgaca ctgtcatcgc aacattatct
    31261 ggtgatcact gcgttgggtg ccgatcgccc tggaattgtg aacaccatca cccgtcatgt
    31321 cagtagttgc ggctgtaata ttgaagacag tcgcctggcg atgctgggag aagagttcac
    31381 gtttatcatg ctgctttccg gttcatggaa tgccatcact ctgattgaat caacgttacc
    31441 gttgaaaggt gccgaactgg atcttttaat cgtgatgaag agcacgacgg cgcgtccgcg
    31501 tccgccaatg ccagcatctg tctgggttca ggtcgatgtt gcagactccc cgcatttaat
    31561 tgaacgcttc acagcacttt tcgacgcgca tcatatgaac attgcggagc tggtgtcgcg
    31621 cacacaacct gctgaaaatg aacgggctgc gcagttgcat attcagataa ccgcccacag
    31681 ccccgcatct gcggacgcag caaatattga gcaagcgttc aaagccctat gtacagaact
    31741 caatgcacaa ggcagtatta acgtcgtcaa ttattcccaa catgatgaac aggatggagt
    31801 taagtaatga atccactgaa agccggtgat atcgcaccga aatttagctt gccggatcaa
    31861 gacggagaac aagttaattt gaccgacttc cagggacagc gtgttctggt ttatttctac
    31921 ccgaaagcca tgacccccgg ctgtaccgta caggcctgcg gcttacgcga taacatggat
    31981 gagttgaaaa aagcgggcgt tgatgtgctg ggtatcagca ccgataaacc cgaaaaactc
    32041 tcccgttttg cggaaaaaga gctgcttaac tttacgctcc tgtctgatga ggaccaccag
    32101 gtgtgcgaac aattcggcgt ctggggtgaa aagtccttca tgggcaaaac ctacgatggt
    32161 attcatcgca tcagcttcct gattgacgct gatggcaaaa tcgaacatgt ctttgacgat
    32221 ttcaaaacca gcaatcacca cgacgttgtg ctgaactggc tgaaagaaca cgcctgatta
    32281 ctttgctcca ttccgtgctg gctgcgcttg cggccagcat acctcacttc tcgtgatcaa
    32341 gatcacattc tcgctttccc ctgcgacacg ggtgtcgaat ccattttttg ctgaacgtta
    32401 atgaccatca tttttgtacc gttcagaatc cagttaatac ataacttatt gaatagattg
    32461 agttaatcag aatggcatcc tttatgcaat atgaaatgca atgtttcata tcattttcaa
    32521 ggagccgaca tgaaccgctt tgtggtggcc gaaccactgt ggtgtacagg atgtaatacc
    32581 tgtctcgctg cctgttcgga cgtgcataaa acgcaaggtt tacagcaaca cccgcgcctg
    32641 gccctggcga agacgtcaac aatcactgcc cctgtcgtgt gtcatcactg tgaggaagcc
    32701 ccttgcctgc aggtctgccc ggtcaatgcc atctctcaga gggatgatgc gatccaactc
    32761 aacgaaagcc tctgtattgg ctgcaagctt tgcgccgtgg tctgcccatt tggcgcaatc
    32821 agcgcttcag gaagccgtcc ggtgaatgcc catgcgcaat atgtttttca ggctgaaggc
    32881 tcactcaaag acggcgaaga aaacgtgccg ccccaacatg cgttgctgcg ctgggaaccg
    32941 ggtgtccaga ccgtcgcggt gaaatgcgac ctgtgtgatt tcttgccaga aggtccggcc
    33001 tgcgttcgcg cttgcccgaa tcaggcgtta cggctgatca ccgacgatag cctgcaacgt
    33061 cagatgaaag aaaaacagcg ccttgccgca agctggtttg ccaatggcgg ggaggatccc
    33121 ctttccctca ctcaggagca acactaatgg atgccctgca attattaacc tggtcgctga
    33181 tcctctatct gtttgccagt ctggcttcgc tgtttttact cggtctggac agactggcta
    33241 ttaagctttc cggcatcaca tcgctggtgt gcggcgtgat tggcatcatc agcggaatta
    33301 cgcaattaca tgctggtgta actttagtcg cccattttgc cacccctttt gactttgccg
    33361 atttaaccct gcgaatggat agcctctcgg catttatggt gctggttatc tccttgctgg
    33421 tggtggtttg ttcgctctat tcattgactt atatgcgcga atacgagggc aaaggcgcgg
    33481 cggcgatggg cttctttatg aatcttttca tcgcatcgat ggttgccctg ctggtgatgg
    33541 acaacgcttt ttggttcatc gtgctgtttg aaatgatgtc gctgtcttcc tggtttctgg
    33601 tcattgccag gcaggataaa acgtcgatca acgctggcat gctctacttt tttatcgccc
    33661 acgccggatc ggtgctgatt atgatcgcct tcttgctgat ggggcgcgaa agcggcagcc
    33721 tcgattttgc cagtttcgca cgctttcact ttctccgggg ctggcgtcgg cggtgtttct
    33781 gctggccttt ttcggttttg ccgcgaaagc cgggatgatg ccgttgcaca gctggttgcc
    33841 gcgcgctcac cctgccgcac catcgcacgc ttcggcgttg atgtctggcg taatggtcaa
    33901 aatcggtatt ttcggcatcc tgaaagtagc gatggatctg ctggcgcaaa cgggtttgcc
    33961 tctgtggtgg ggcattctgg tgatggcgat cggcgcaatc tccgcgctcc tgggcgtgct
    34021 atatgcgctg gcggaacagg atatcaaacg gctgctggcc tggagtaccg tcgaaaacgt
    34081 cggcattatt ttgctggcag tcggtgtggc gatggacggt ctgtcactgc acgacccgct
    34141 gctcaccgtg gttggactgc tcggcgcact gtttcatctg ctcaaccatg cgctgttcaa
    34201 agggctgcta tttctcggcg cgggcgcgat tatttcgcgt ttgcataccc acgacatgga
    34261 aaaaatgggg gcactggcga aacggatgcc gtggacagcc gcagcatgcc tgattggttg
    34321 cctcgcgata tcagccattc ctccgctgaa tggttttatc agcgaatggt acacctggca
    34381 gtcgctgttc tcactaagtc gtgtggaagc cgtagcgcta caacttgcgg gtcctattgc
    34441 tctggtaatg ctggcagtca ctggtgggct ggcagtaatg tgcttcgtca aaatgtacgg
    34501 tattactttc tgtggtgcgc cgcgcagtac acacgctgaa gaggcacagg aagtgccaaa
    34561 tacgatgatc gtcgccatgc tactgctcgc ggcactctgc gtattaattg cgcttagtgc
    34621 cagttggctg gcaccgaaga taatgcatat tgcccatgcg tttaccaata cccctcccgt
    34681 cactgtcgcc agcggaatag cacttgtacc cggcacgttt catacacggg tcactccttc
    34741 attactgttg ctgttactac tggcgatgcc tttgctgcct ggcctttact ggctgtggtg
    34801 tcgttcgcgc cgcgcagcgt ttcgtcgcac aggagatgcc tgggcatgcg gctacggctg
    34861 ggaaaacgcg atggctccgt caggcaatgg cgtgatgcag ccgctgcgtg tggtcttctc
    34921 tgcgttattt cgtctacgac aacagctcga ccctacgctg aggctaaata aaggtcttgc
    34981 gcacgtcacc gccagggctc agagcacaga acccttctgg gatgagcggg tgatccgccc
    35041 catcgtgagc gccacccaac ggctggtcaa agaaatacag catctgcaaa gcggcgactt
    35101 tcgtctctat tgcctgtatg tggtcgccgc actggttgtg ctgctaatcg ctattgccgt
    35161 ctaaggaaat caccatgaga caaactcttt gcgacggata tctggtcatt tttgcgttag
    35221 cacaggccgt gattctgctg atgctaaccc cactttttac gggtatttcc cggcagatac
    35281 gcgcgcgtat gcactcccgc cgcgggccgg ggatctggca ggattatcgc gatatccaca
    35341 aactgtttaa acgccaggaa gttgcgccga catcttcagg tctgatgttc cgcctgatgc
    35401 cgtgggtatt aatcagcagc atgctggtgc tggcgatggc cttaccacta tttattaccg
    35461 tttccccttt tgcgggcggc ggcgatctga tcacccttat ctatcttctt gccctgtttc
    35521 gttttttctt tgctctttcc gggctggata ccggaagtcc gtttgcggga gtcggtgcca
    35581 gtcgcgagtt gacgctcggc attctggtcg aaccaatgct tattctctca ctgctggtat
    35641 tggcgctgat agcaggttcc acgcatatcg agatgatcag caatacgctg gcgatgggct
    35701 ggaactcgcc gctaaccacc gtactggcgt tactggcctg tggttttgcc tgcttcattg
    35761 agatgggaaa aattcccttt gatgttgctg aagcagaaca ggaattacag gaaggcccgc
    35821 tgaccgaata ttccggtgcc gggctggcgc tagcgaaatg ggggctgggg ctgaaacagg
    35881 tcgtgatggc agcactgttt gtggccctgt ttctgccctt tgggcgcgcg caagaacttt
    35941 ctctcgcctg cctgctgact tcacttgtcg ttacgctgct caaggttttg ctgatttttg
    36001 tactggcctc aatcgcagaa aacacgctgg cacgcgggcg ttttttactc attcaccatg
    36061 tgacctggct tggcttcagc cttgctgcgc ttgcatgggt cttctggtta accggtctgt
    36121 aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct gccttttatc
    36181 ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg ggttttgttc
    36241 gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca ggccgataaa
    36301 gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattgtttgg cctggtcatt
    36361 gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct ggtcacgatc
    36421 tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg cacgaatcgt
    36481 tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact ctcctcgacg
    36541 ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc gttgatcagt
    36601 tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat cacccatatc
    36661 ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg aacgtttgtg
    36721 cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg cggcatcctg
    36781 tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc ggacgcaatg
    36841 gaagcgccaa caccgatcag cgcctatctc cacgccgcat cgatggtgaa agtgggcgtt
    36901 tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat tggcggcgtt
    36961 ggcatggtca tggcactggt caccattctt tatggctttc tgatgtattt gccacagcag
    37021 gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat gttcttcggc
    37081 ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc ctacatcgtc
    37141 aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag ttacagctgc
    37201 ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt gccaggcgtg
    37261 ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg cttcttcagt
    37321 aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat cctgctgccc
    37381 gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat tcgctggttc
    37441 ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc gctgctaggg
    37501 tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag cgtaatcgcc
    37561 gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta aataatctgg
    37621 cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat cgcctgtcat
    37681 gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact ctctcgtgcc
    37741 tgttcgccgc agagcaactg ctgatctggt ccgccagcgc ctttatcacc aaagtgctgc
    37801 tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc ccggaaaaag
    37861 cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg ctttgcgcat
    37921 ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg ctggcggtag
    37981 cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccagcgcaat atcctgcggc
    38041 aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg cttcttgcct
    38101 ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc gccgtcattg
    38161 tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac gtgaacaact
    38221 tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt actcctgctc
    38281 acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct ctctgcgact
    38341 tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct ggcactctgg
    38401 gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg gcttcatatt
    38461 gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct caccggtgtt
    38521 tactcgattg gctacatgct tcatgaagtg gcacgcggcg agctttcacc cgttacactg
    38581 tgcgattact acggtttctt ccatctgttt ttgttcacca tgctgctggt tgttaccagc
    38641 aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc ggcgtttctg
    38701 gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat cattatttgt
    38761 actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa cgccgccagc
    38821 gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca atcgtccttg
    38881 cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt tggtaccaaa
    38941 accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc gccgagtccg
    39001 gtcagcgccc tgctctccgc cgtattgctg aactgcgcgc tgttggtgct gattcgctat
    39061 tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt gctcatcttc
    39121 ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga cattaagcgt
    39181 ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct aggcattggc
    39241 gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct ggcaaaaacg
    39301 ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga tctcaacgtc
    39361 gtctgtggga tactcaaaat catgccattt accgccgtgc tgtttggcgg cggtgcgctg
    39421 gcgctggcag ggatgccgcc cttcaacatt tttcttagcg aatttatgac cgttaccgca
    39481 ggactggcac gtaatcacct gctgattatc gtcctgctgt tattgctgtt aacgctggtg
    39541 ctggcgggcc tggtacggat ggctgcgcgg gtgttaatgg cgaaaccgcc gcaggccgtt
    39601 aaccggggtg atctcggctg gttgaccacc tcgccaatgg tgattctgct ggtcatgatg
    39661 ctggcgatgg gaacgcatat tccacaacct gtcatcagga tcctggcggg cgcttccact
    39721 atagtcctct cagggacgca cgacctgcct gcacaacgta gcacctggca tgattttttg
    39781 ccttcaggca ccgcatctgt ttcggagaaa cacagtgaac gttaattcat cgtcaaatcg
    39841 tggcgaagcg attctcgccg ccctgaaaac gcagttcccc ggcgcggtgc tggatgaaga
    39901 gcgacaaacg cctgaacagg tcaccattac ggtgaaaatc aatctgctgc ctgacgttgt
    39961 acattatctt tattatcaac atgatggctg gcttccagtc ctgtttggca acgacgagcg
    40021 gacacttaac ggtcattacg cggtttatta tgccctttca atggaagggg ccgaaaaatg
    40081 ctggatcgtg gtgaaggcac tggtcgatgc cgacagtcgg gagtttccgt cagtcacacc
    40141 gcgcgtccct gccgcggtct ggggcgagcg agaaattcgc gatatgtacg ggctgattcc
    40201 ggttggcctg ccggatcagc gtcgcctggt gttgcccgat gactggccgg aagatatgca
    40261 tccgctgcgc aaagatgcga tggattatcg actgcgccct gaaccgacga cagattccga
    40321 aacgtatgcg tttatcaatg agggcaacag cgatgcgcgg gtgatccctg tcggcccgct
    40381 gcatatcacc tcctatgaac cgggtcactt ccgcttgttt gtggatggcg agcaaattgt
    40441 cgatgctgat taccgcctgt tttatgtcca tcgcggcatg gagaaactgg cagaaacgcg
    40501 gatgggctac aacgaagtga ccttcttatc ggaccgcgtg tgtgggattt gcggttttgc
    40561 ccacagtgtg gcctatacca actcggttga aaatgcactg gggattgagg tgccgcaacg
    40621 agcacatact attcgctcga ttctgctgga agtcgagcag ctacacagtc atttgctcaa
    40681 ccttggcctc tcctgccatt ttgttggttt tgataccggc tttatgcaat ttttccgcgt
    40741 gcgggaaaag tcgatgacga tggcggaatt gctgaccggg tcgcgtaaaa cctacggtct
    40801 gaatctgatt ggtggtgttc gccgcgatat tctcaaagag caacgtctgc aaacgctgaa
    40861 actagtgcgc gagatgcgcg ccgacgtgtc ggagctggta gagatgctgc ttgctacgcc
    40921 gaatatggaa caacgcactc agggcattgg cattctcgac cgacaaatcg cccgtgatta
    40981 tagccctgta gggccgctga tccgcgcagt ggttttgccc gtgatttgcg ctttgatcac
    41041 ccctacgccg actacggcaa tattccaaaa acactgttta cctttaccgg cggcgatgtt
    41101 ttctcccgcg tgatggtccg tgtcaaagag acgtttgatt cgctggcaat gctggaattt
    41161 gccctcgaca acatgccgga tactccactg ctgaccgaag gctttagcta taaacctcac
    41221 gcattcgcgc tgggctttgt tgaagcgcca cgcggtgaag acgtgcactg gagcatgctc
    41281 ggtgataacc aaaaattgtt ccgctggcgc tgtcgtgccg ccacctacgc caactggccg
    41341 gtgttgcgtt acatgctgcg cggcaatacc gtttctgacg caccgctgat tatcggtagc
    41401 cttgatccct gctactcctg taccgaccgt gtgacgctgg tagatgtgcg caagcgccag
    41461 tcaaaaaccg tgccgtataa agagatcgaa cgctacggca ttgatcgtaa ccgttcgccg
    41521 ctgaagtaag gacagaagat gctgaagtta ctgaaaacta ttatgcgcgc cggaaccacg
    41581 acggtgaaat atcccttcgc gccactggag gtcagcccag gctttcgcgg gaaaccggac
    41641 ctgatgccca gccaatgtat tgcctgcggt gcctgcgcct gtgcctgtgc ttgtccggca
    41701 aatgcgctga ctatccagac cgacgaccag caaaattcgc gcacctggca gctctatctg
    41761 gggcgttgta tttactgcgg acgttgtgaa gaagtgtgcc cgaccagagc catccagctt
    41821 accaataact ttgaactgac cgtcacccat aaagccgatc tctataccca cgcgacgttc
    41881 catctacaac gttgcagccg ttgcgaacgc ccgtttgccc cgcaaaaaac cgtcgcactg
    41941 gctgctgaat tgttggcaca gcaacaaaat gcgccacaaa accgcgaaat gttatgggcg
    42001 caagcgagcg tctgcccgga gtgcaaacaa cgcgcgacgc tgatcaacga cgatacagat
    42061 gtaccgctgg tggctaagga gcagctatga gtccagtgct tacgcaatat gtcagccagc
    42121 ccatcacgct ggacgagcaa acgcaaaaga tgaagcggca tttgctacag gatatccgtc
    42181 gctcggctta cgtttatcgc atcgattgcg gcggctgcaa cgcctgtgaa atcgaaattt
    42241 ttgctgccat cacccccgta ttcgacgcag aacgttttgg cattaaggtt gtttcatcac
    42301 cgcgtcacgc cgatattttg ttatttactg gcgcagtcac ccgggcgatg cgtatgcctg
    42361 cacttcgggc gtatgagtct gcccccgatc ataaaatctg tgtttcctac ggcgcgtgcg
    42421 gtgtcggcgg cggtattttc cacgatctct acagcgtctg gggcggcagc gacaccattg
    42481 tccccattga tgtttggatc cccggctgcc cgccaacacc ggccgccacc attcacggtt
    42541 tcgccgtggc gctcggtttg ctgcaacaga agattcacgc ggtggattat cgcgatccca
    42601 ccggggtgac tatgcaaccg ttgtggccgc agatcccgcc atcacagcgt atcgccattg
    42661 agcgagaagc gcggcggctg gcgggctatc gtcaggggcg agaaatttgc gatcggctcc
    42721 tgcgccattt aagcgacgat cctacaggaa atcgggttaa cacctggttg cgcgatgccg
    42781 acgatccacg tctcaatagt atcgttcagc aactctttcg cgtactccgg gggttacatg
    42841 actgaagagt gcggggaaat tgttttctgg acgctgcgaa aaaagtttgt cgccagtagc
    42901 gacgagatgc cggaacacag ctctcaggta atgtattact cgctggctat cggtcatcac
    42961 gttggcgtga ttgattgtct gaatgtcgcc ttccgctgcc cactgacgga atacgaagat
    43021 tggcttgcac tggtcgaaga ggagcaagcc cgacgtaaga tgctgggggt gatgactttt
    43081 ggtgagattg ttattgacgc cagccacacc gccctgttga cccgggcatt cgcgccactg
    43141 gcagatgacg cgacgtctgt gtggcaagcg cgtagcattc aattcattca tctgttggat
    43201 gaaattgtgc tggaaccggc tatctatctg atggccagaa aaattgcgtg agaaggattt
    43261 ctcattaata aggactgttg atggctatgt cagacgaagc gatgtttgcc ccgccgcaag
    43321 gaataacaat tgaagcggta aacggaatgc tcgcggagcg gttagcgcag aaacacggca
    43381 aggcgtcttt attacgcgcc ttcatcccgc tgccgccgcc gttcagcccg gtacaactta
    43441 ttgaactgca tgttctcaaa agcaacttct attaccgcta ccatgatgat ggcagcgatg
    43501 tgacggcaac aacagagtat cagggcgaga tggtcgatta ttcgcgtcac gccgtccttc
    43561 tcggcagtag tggaatggcg gagctacgct ttattcgtac ccacggcagc cgttttactc
    43621 cccaggattg cacactgttt aactggctgg ctcgcattat caccccggtt ctgcaatcat
    43681 ggctcaatga tgaagaacag caggtggcgc tgcgtttgct ggagaaagat cgcgatcatc
    43741 atcgggtact ggtggatatc actaatgcag tgctgtcaca tcttgatctc gacgatctga
    43801 tcgctgacgt cgctcgtgag atccatcatt ttttcggtct ggcttcagtc agtatggtac
    43861 tgggcgatca tcgaaagaac gagaagttca gcctgtggtg cagcgatctt tctgcctcac
    43921 attgtgcgtg tctgccacgc aatatgcctg gcgacagtgt attgctgaca caaacgctac
    43981 aaacccgaca accgaccttg acgcaccgtg cagatgatct gtttctctgg caacgcgacc
    44041 cgttattact cttacttgca tctaacggct gcgaatctgc gctccttata ccgcttacct
    44101 ttggcaacca tacaccgggt gcattgttgc tggcgcatac ctcttccact ctctttagtg
    44161 aggaaaactg ccagctacta caacacatag ccgatcgcat cgctattgcc gttggcaatg
    44221 ccgatgcctg gcgtagaatg accgatttgc aggaaagttt gcagcaagaa aaccaccagc
    44281 ttagcgagca gctcctttcg aatctgggcg tcggtgacat tatctatcaa agccaggcaa
    44341 tggaagagct gctccagcag gtagatattg tggcgaagag cgacagtacg gtgttgattt
    44401 gcggtgaaac cggaaccggc aaagaggtga tcgccagagc gatccatcaa cttagcccgc
    44461 gacacgacaa gccgctggtc aaaatcaact gcactgccat ccccgccagt cttctggaaa
    44521 gtgagttatt cggtcatgac aaaggggcgt ttactggtgc gattaatacc catcgtggtc
    44581 gttttgaaat tgccgatggc ggcacgttgt ttctcgatga aattggcgat ctgccgttag
    44641 aacttcagcc taaactgctg cgcgtattgc aggaacggga gattgagcgt ctcggcggga
    44701 gtagaacgat cccggtaaat gtcagagtca ttgccgccac caaccgtgat ttgtggcaaa
    44761 tggttgaaga tcgccagttt cgcagcgatc tcttttatcg cctgaatgtc ttcccactgg
    44821 aattgcctcc gctgcgcgac cgtccggaag atatccctct tttagcaaag catttcacgc
    44881 aaaaaatggc gcgccatatg aatcgttcaa ttgacgccat cccgaccgag gcgctacgcc
    44941 agttgatgtc gtgggactgg ccgggcaacg tgcgcgagct ggaaaacgtg attgagcgag
    45001 cggtactgct gactcgcggt aacagtctga atttacatct taatgtccga caaagccgtt
    45061 tactgccgac gctaaatgaa gattcagcgc ttcgcagttc aatggcgcag ttgctgcacc
    45121 cgacgacgcc agagaatgac gaagaagaac gtcagcgcat tgttcaggta ttgcgagaaa
    45181 ccaatggcat tgttgccggg ccccgtggcg cggcgacacg attagggatg aagcgcacca
    45241 cgctgctgtc acgaatgcag cgtctgggaa tctcggttcg cgaggtgttg taatctgctt
    45301 ttgcaggagt atgcatgaga aacaaactct ctttcgactt gcagttgagc gccagaaaag
    45361 cggcaatcgc tgaacggatt gccgcccata aaattgcccg cagtaaagtg tcggtctttt
    45421 taatggcgat gtccgctggc gtgtttatgg cgatcggatt tactttttac ctttccgtta
    45481 tcgccgatgc cccgtcttca caggcattaa cccatctggt gggcggcctt tgctttacac
    45541 ttggctttat tttgctggcg gtttgcggca ccagcctgtt cacctcgtcg gtaatgacgg
    45601 taatggcaaa aagtcggggc gttattagtt ggcgaacttg gctgattaac gcacttctgg
    45661 tggcctgcgg taatctggca ggtattgcct gtttcagttt gttaatctgg ttttccgggc
    45721 tggtgatgag tgaaaacgcg atgtggggag tcgcgatttt acactgcgcc gagggcaaaa
    45781 tgcatcatac atttactgaa tctgtcagcc tcggcattat gtgcaatctg atggtttgcc
    45841 tggcgctgtg gatgagttat tgcggacgtt cgttatgcga caaaatcgtc gccatgattt
    45901 tgcccatcac cctgtttgtc gccagtggct ttgagcactg tatcgccaat ttgtttgtga
    45961 ttccgttcgc cattgccatt cgccatttcg cccctacctc cttctggcaa ctggcgcaca
    46021 gtagcgcaga caattttccg gcactgacgg tcagccattt tattaccgcc aatctgctcc
    46081 cggtgatgct gggtaatatt atcggcggtg cagtgctggt gagtatttgt tatcgggcta
    46141 tttatttacg tcaggaatcc tgaaaaatca gcccggcgaa acagttcgtc gggctgaaga
    46201 ttattcttgc gcgatttgcc catcgggcca ggcgtggatc acggctttga tcagcgtcgc
    46261 caatggaatg gcgaagaata cgccccagaa tccccacaaa ccaccgaaga tcaccaccga
    46321 taaaataatc accagcggat gcaggttaac cgcttcggag aacaacaccg gtactagcag
    46381 gttgccgtcc agcgcctgaa taatcagata caccgcgaag cagctccaga attcagtacc
    46441 tgcgccaaac tggaatagcg ccacgccaac caccggaatg gtcaccacaa atgcgccaat
    46501 gtacggaata agaaccgaga agccgaccag cactgccagc agcagcgaat agttcagccc
    46561 gaagagcaag aaccccagcc aggtggcgat ccccaccacg atcatctcca gcactttgcc
    46621 gcggatatag ttggtgattt gttgattcat ctccttccac acctgtcctg ccagcccacg
    46681 gttacgcggc agcacccggc gaacggcgtt cagcatctgc tctttgtctt tcagcaggaa
    46741 gaagaccatc aatggcacca gcaccagata gacggctatg gtcagcaaac cgaccagcga
    46801 ggcgagggaa attttcacca ccgaatcgcc catggtcagc atccgactgc gcatattttc
    46861 ggccattgca tcaataatgc ccgcgtccat taacgccgga tagcggcgcg gcaacgtggc
    46921 ggcaaagtca gaaagcttat tgagcatccc cggcatatcg cggattaagt agatgccctg
    46981 ttgccaggcg ataggcagta ccacgaacgc catcagtagc aatataccga caaaaaccac
    47041 caatacaatc gacgtcgccc agcggcggga gcagccaata gattgcaggc gcacggttgg
    47101 ccattccagc aaataagcca gcacaatagc caccagcaac ggagcaagca ggccactaaa
    47161 gaaaaagata atgccaaatc cggcaactaa aataaccagc aaggcaatcg cttccgggtc
    47221 gctaaaacgg cggcgatacc attgcatcaa catttcgagc ataaagacct tccctgaacc
    47281 tcaagagcgg gattgcgatc cgcaattgta tcgaaatgtc acaaaaaaga cttcgctttt
    47341 tatgacggat tcaggaaact gaaaagtcat ttgagtgggc taatcttcgc cgttacactc
    47401 aaaggcggcg aggtgggaac gatatttcac agtatcggtc aaatgactat cttcagaaat
    47461 acaggataga ggttatgttc aggcagttga aaaaaaacct ggttgcaacc ctcattgctg
    47521 ctatgaccat tggtcaggta gccccggcgt ttgccgacag cgcagacacc ttgccggata
    47581 tgggaacctc cgcaggaagc acgctttcca ttggtcagga aatgcagatg ggcgactatt
    47641 atgtccgcca gctacgcggc agcgcgccgt taattaatga cccgctgtta acgcaatata
    47701 ttaattcgct ggggatgcgt ctggtttcgc atgccaattc ggttaagaca ccgtttcatt
    47761 tttttctgat caacaacgac gaaattaacg cctttgcttt ctttggcggc aacgtggtgc
    47821 tgcactctgc cctgttccgt tattccgata acgaaagtca actggcttca gttatggcgc
    47881 acgaaatctc ccacgtcacc caacgtcacc tggcgcgagc gatggaagat caacaacgca
    47941 gcgcgccgct gacctgggtc ggcgcgttag gttctatttt actggcgatg gccagtccgc
    48001 aggcggggat ggcggcgctg accggtacac tggcgggaac gcgccagggg atgatcagtt
    48061 tcacccagca aaatgaacag gaagcggacc gcattggtat tcaggtgctg caacgctcgg
    48121 gattcgatcc acaggcgatg ccgaccttcc tggaaaaatt gctcgatcag gcgcgttact
    48181 cctcgcgccc gccggaaatt ttattgactc acccgttgcc ggaaagtcgt ctggcagatg
    48241 cccgcaaccg tgctaatcag atgcgcccga tggtggtgca gtcgtcggaa gatttctatc
    48301 tggcgaaagc gcgcacactg gggatgtata attccggacg taaccagctc accagtgatt
    48361 tgctggatga atgggcgaaa ggaaacgttc gtcagcaacg agcggcgcaa tatggtcgtg
    48421 ctttacaggc gatggaagcc aataaatacg acgaggcgcg taaaacgctg caaccgttac
    48481 tggcggcaga acctggcaac gcatggtatc tcgatctggc taccgatatc gatcttgggc
    48541 aaaacaaagc caatgaggca atcaatcgcc tgaaaaatgc ccgcgattta cgcaccaatc
    48601 cggtgttgca gctcaacctg gcgaacgctt atctgcaagg cggtcaacca caagaagcgg
    48661 ccaatattct gaatcgctac acctttaata ataaagatga cagcaacggc tgggatttgc
    48721 tggcacaggc ggaagccgcg ctaaataacc gcgatcagga gctggctgcg cgagcagaag
    48781 gttatgcgct cgccggacga ctcgatcagg ccatttcgct gttgagtagc gccagttcgc
    48841 aggtgaaatt aggcagcctg caacaagcgc gttacgatgc gcgcatcgac cagttgcgcc
    48901 agctgcagga acgctttaag ccttatacca agatgtaatt cgcattaaag gaagaacaat
    48961 gaccaaacag gtaaaaatct accataaccc acgctgttca aagagccggg aaacgctgaa
    49021 cctgctgaaa gaaaacggcg tggagccaga agtggtgctc taccttgaga cacccgccga
    49081 tgcggcaacg ctgcgcgatt tgctgaaaat gctggggatg aatagcgccc gtgaattgat
    49141 gcgccagaaa gaggatcttt ataaagaact aaacctggct gacagttcgc ttagcgaaga
    49201 ggcgctgatt caggcaatgg tggataatcc gaagctgatg gagcgcccga ttgtggtcgc
    49261 gaatggcaaa gcgcggattg gtcgtccacc ggagcaggta ctggaaatcg tgggttaaga
    49321 tgtgctccat cgctagttga agcacattgc cggatgcgac gcttgccacg tcttatccgg
    49381 cctacgaata ctatggatta acttcgcgta ggtcaggcgt tcgcgccgca tccgacaata
    49441 aacaccttat ctacaacttc agaatttctt tcacaaacgg aatggtcagc ttacgttgcg
    49501 cggtaatcga cgcacgatcc aactgatcca acgtcataaa tagcgtgcgc atttctctgt
    49561 cgagccgctt cagcaagaaa cgccccacat cttccggcag ttcaaaacca cgcaaacgcg
    49621 cgcgtaactg tagcgcctgc aacttatctt catcagaaag tggctgcaat ttgtagatct
    49681 gcccccagtc gagacgcgac gcgagatccg gtaatcccag attcaactgc cgcggtggac
    49741 gatcgccggt gatcaacaac cgtgttttgc ccgattccag aattcgattg tagagatcga
    49801 aaatcgccat ctcccacaac tcatcgcctg caatacactc aatgttgtcg atacagacca
    49861 gcgacaaatg ctccataccg tcgagcactt ccggaacaaa ccaggtgcgt ttatccagcg
    49921 ggacatagcc caccgcatcg ccacgctgcg acaattccgc gcaagccgcg tgcagcagat
    49981 ggctgcgccc cgcgccttcg cgtgcccaga gatagatgta accgctatgt tcctgacgca
    50041 gcacgttttg cagcgcggcc agtaaagagg agttatcccc cggccagaaa cttgcaaagg
    50101 tttcgtcgtc aggaagataa agtggcaaag agagctgtgc cggtgtgttc agagatacct
    50161 caaccaggat ttcacaaaat cgcgagaagt ttaccacaga atcccatgat gtttgaaccg
    50221 ggcagcaaca ctgcccggtc ggtacattat ttgtctgtta tatccgcgtc ttctgcgtcc
    50281 agcaccactt cttcagggcg cagcacactg atcagtttga aaatcaaact taagccgata
    50341 ccgacgatgg tcgccagcgc catacctttt aactccgccg cgccgatgtt taccttcgcg
    50401 ccactgacgc cgatgatcaa aatcacggaa gtcaggatca ggttctgtgc tttgttgtaa
    50461 tccactttcg attcgatcaa aacacgaata ccggaagcac caatgacacc ataaagcagc
    50521 agcgaaacgc cgcccatcac cggcaatggg atcatctgga tagcggcagc cagtttaccg
    50581 acgcaggaaa gcaggatagc gaaaatcgcc gccccgccga taacccaggt actgtaaaca
    50641 cgggtgatcg ccatcacgcc aatgttttct ccgtaagtgg tatttggcgt agagccaaag
    50701 aagccggaga tcacggtcga caggccgtta gcaaacatcg aacggtgcag acctggatcg
    50761 cgcagcagat cttttttgac gatattagcc gttactacca ggtgccctac gtgttcggca
    50821 ataaccacta acgccgctgg cagaatagtc agaatggcaa accactcgaa gcgcggcgta
    50881 tagagggttg gcagtgcaaa ccagtgagca ttaataatcg gcgtggtatc gacaattccc
    50941 attgcgaaag agagcgcgta ccccaccagc acgccaatta aaatcgggat aattgccagg
    51001 aaaccacgaa acagcacgga acctaaaacc gtgaccgcca gggtggtaat agagatgatg
    51061 atggttttgg agtttggcgt ttgcccttca gccgggagta aacccgccat accggcagct
    51121 acgcccgcca gctccagacc gatgacggca acgattgcgc ccattgccgc aggtggaaac
    51181 agcacgtcca accagccggt ccccgctttc ttcacgataa aagaaaccag gcagaacagc
    51241 acgccgcaca taataaagcc gcccagcgcg acttcatacc ctaacggcaa cagtaacaat
    51301 accggtgaaa taaaggcaaa gctggaacca agataagccg gaattttccc tttacagatg
    51361 aagagataca gcagcgttcc aataccgtta aataacagta cagtcgccgg gttaatatga
    51421 aataagacgg gcaccaggac ggttgcacca aacatggcga acaaatgttg caaactaagc
    51481 gggattgtct gtaaaagtgg cggtctttca ctcaccccga tagcacggcg cgtcatagta
    51541 ttatcctctg tattatgtgt tataggcgct ttactcaaaa aaaagccgac tcttaaagtc
    51601 ggctttaatt atttttattc tttatttcgt accaaagatt ttgtcaccgg catcgccgag
    51661 gcccggaata atgtatccgt gctcgttcag tccctggtca atcgatgcag tatacagttc
    51721 gacgtccggg tgcgcttttt ccagcgcagc gataccttct ggcgcagcga ccagcaccag
    51781 aactttgatg ctgctgcagc ccgctttttt cagcaggtcg atggtcgcga taacggaacc
    51841 accggttgcc agcattgggt caacgatcag cgccatacgc tcatcgatgt tagaaaccag
    51901 tttctggaag tacggtaccg gctccagcgt ttcttcatta cggtacatac cgacaacgct
    51961 gatgcgcgcg ctcggaacgt tttccagcac accgtccatc ataccaagac ccgcacgcag
    52021 aattggcaca acggtaattt tcttaccttt gatctggtcg atttctaccg ggccgttcca
    52081 gccttcgata gttacttttt ccgtttcgag gtcggcggtc gcttcgtaag tcagcaggct
    52141 acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg atatcttgct cacgcatcag
    52201 tcccagcttg tgtttgacga gtgggtgttt gacttccacg atcttcatac tctttctcct
    52261 ttgaggggca gccacaaaaa aatcgacgga ttatacctcc tttcttcaag gcggcaatat
    52321 tattttcgtt gactttagtc aaaatgataa cagtttgaga taaagttatt ttatattcaa
    52381 atggttatga aagaagatta ttccatccga aaactaacct ttgccctggc acaagtcttc
    52441 tttcgccgcg cgcccgggga aaagacgtgc aaaaaggttg tgtaaaacag tctcgcaaac
    52501 gtttgctttc cctgttagaa ttgcgccgaa ttttattttt ctaccgcaag taacgcgtgg
    52561 ggacccaagc agtgaccgat aaaacctctc ttagctacaa agatgccggt gttgatattg
    52621 acgcgggtaa tgctctggtt ggaagaatca aaggcgtagt gaagaaaacg cgtcgtccgg
    52681 aagtgatggg cggtctgggc ggcttcggtg cactgtgtgc attgccgcaa aaatatcgtg
    52741 aacccgtgct ggtttccggc actgacggcg taggtaccaa gctgcgtctg gcaatggact
    52801 taaaatgtca cgacaccatt ggtattgatc tggtcgccat gtgcgttaat gacctggtgg
    52861 tgcaaggtgc ggaaccgctg tttttcctcg actattacgc aaccggaaaa ctggatgttg
    52921 ataccgcttc agcggtgatc agcggcatcg cggaaggttg tctgcaatcg ggctgttctc
    52981 tggtgggtgg cgaaacggca gaaatgccgg ggatgtatca cggtgaagat tacgatgtcg
    53041 cgggtttctg cgtgggcgtg gtagaaaaat cagaaatcat cgacggctct aaagtcagcg
    53101 acggcgatgt gctgattgca ctcggttcca gcggtccgca ctcgaacggc tattcgctgg
    53161 tgcgcaaaat tcttgaagtc agcggttgtg atccgcaaac caccgaactt gatggtaagc
    53221 cattagccga tcatctgctg gcaccgaccc gcatttacgt gaagtcagtg ctggagttga
    53281 ttgaaaaggt cgatgtgcat gccattgcgc acctgaccgg cggcggcttc tgggaaaaca
    53341 ttccgcgcgt attgccagat aatacccagg cagtgattga tgaatcttcc tggcagtggc
    53401 cggaagtgtt caactggctg caaacggcag gtaacgttga gcgccatgaa atgtatcgca
    53461 ccttcaactg cggcgtcggg atgattatcg ccctgcctgc tccggaagtg gacaaagccc
    53521 tcgccctgct aaatgccaac ggtgaaaacg cgtggaaaat cggtatcatc aaagcctctg
    53581 attccgaaca acgcgtggtt atcgaataat gaatattgtg gtgcttattt ccggcaacgg
    53641 aagtaattta caggcaatta ttgacgcctg taaaaccaac aaaattaaag gcaccgtacg
    53701 ggcagttttc agcaataagg ccgacgcgtt cggccttgaa cgcgcccgcc aggcgggtat
    53761 tgcaacgcat acgctcatcg ccagcgcgtt tgacagtcgt gaagcctatg accgggagtt
    53821 gattcatgaa atcgacatgt acgcacccga tgtggtcgtg ctggctggtt ttatgcgcat
    53881 tctcagcccg gcgtttgtct cccactatgc cgggcgtttg ctgaacattc acccttctct
    53941 gctgccgaaa tatcccggat tacacaccca tcgtcaggcg ctggaaaatg gcgatgaaga
    54001 gcacggtaca tcggtgcatt tcgtcaccga tgaactggac ggtggcccgg ttattttaca
    54061 ggcgaaagtc ccggtatttg ctggtgatac ggaagatgac atcaccgccc gcgtgcaaac
    54121 ccaggaacac gctatttatc cactggtgat tagctggttt gccgatggtc gtctgaaaat
    54181 gcacgaaaac gccgcgtggc tggatggtca acgtctgccg ccgcagggct acgctgccga
    54241 cgagtaatgc ccccgtagtt aaagcgccag ctctgccgct ggcgtttttc aattcacctg
    54301 taaatcgcaa gctccagcag tttttttccc ccttttctgg catagttgga catctgccaa
    54361 tattgctcgc cataatatcc aggcagtgtc ccgtgaataa aacggagtaa aagtggtaat
    54421 gggtcaggaa aagctataca tcgaaaaaga gctcagttgg ttatcgttca atgaacgcgt
    54481 gcttcaggaa gcggcggaca aatctaaccc gctgattgaa aggatgcgtt tcctggggat
    54541 ctattccaat aaccttgatg agttctataa agtccgcttc gctgaactga agcgacgcat
    54601 cattattagc gaagaacaag gctccaacgc tcattcccgc catttactgg gcaaaattca
    54661 gtcccgagtg ctgaaagccg atcaggaatt cgacggcctc tacaacgagc tattgctgga
    54721 gatggcgcgc aaccagatct tcctgattaa tgaacgccag ctctccgtca atcaacaaaa
    54781 ctggctgcgt cattatttta agcagtatct gcgtcagcac attacgccga ttttaatcaa
    54841 tcctgacact gacttagtgc agttcctgaa agatgattac acctatctgg cggtggaaat
    54901 tatccgtagc gatagcatcc gttacgcgct gctggagatc ccatcagata aagtgccgcg
    54961 ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag ccgatgattc ttctggataa
    55021 cattctgcgt tactgccttg atgatatttt caaaggcttc tttgattatg acgcgctgaa
    55081 tgcctattca atgaagatga cccgcgatgc cgaatacgat ttagtgcatg agatggaagc
    55141 cagcctgatg gagttgatgt cttccagtct caagcagcgt ttaactgctg agccggtgcg
    55201 ttttgtttat cagcgcgata tgcccaatgc gctggttgaa gtgttacgcg aaaaactgac
    55261 tatttcccgc tacgactcca tcgtccccgg cggtcgttat cataatttta aagactttat
    55321 taatttcccc aatgtcggca aagccaatct ggtgaacaaa ccactgccgc gtttacgcca
    55381 tatttggttt gataaagccc agttccgcaa tggttttgat gccattcgcg aacgcgatgt
    55441 gttgctctat tatccttatc acacctttga gcatgtgctg gaactgctgc gtcaggcttc
    55501 gttcgacccg agcgtactgg cgattaaaat taacatttac cgcgtggcga aagattcacg
    55561 catcatcgac tcgatgatcc acgccgcaca taacggtaag aaagtcactg tggtggttga
    55621 gttacaggcg cgtttcgacg aagaagccaa cattcactgg gcgaagcgcc tgaccgaagc
    55681 aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt cacgccaaac tgttcctgat
    55741 ttcacgtaaa gaaaacggtg aagtggtccg ttacgcacac atcgggaccg ggaactttaa
    55801 cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg accgcagatg cgcgtatcac
    55861 caacgaagta cggcgggtat ttaactttat tgaaaaccca taccgcccgg tgacatttga
    55921 ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta tatgaaatgg tagaccgcga
    55981 aatcgccaac gcgcagcaag ggctgcccag tggtatcacc ctgaagctaa ataaccttgt
    56041 cgataaaggc ctggttgatc gtctgtatgc ggcctccagc tccggcgtac cggttaatct
    56101 gctggttcgc ggaatgtgtt cgctgatccc caatctggaa ggcattagcg acaacattcg
    56161 tgccatcagt attgttgacc gttaccttga acatgaccgg gtttatattt ttgaaaatgg
    56221 cggcgataaa aaggtctacc tttcttccgc cgactggatg acgcgcaata ttgattatcg
    56281 tattgaagtg gcgacaccgc tgctcgatcc gcgcctgaag cagcgggtgc tggacatcat
    56341 cgacatattg ttcagcgata cggtcaaagc acgttatatc gataaagaac tcagtaatcg
    56401 ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag ttggcgattt acgactacat
    56461 caaatcactc gaacaacctg aataacccta tgccaataca cgataaatcc cctcgtccgc
    56521 aggagtttgc tgcggtcgat cttggctcaa acagttttca catggtcata gcccgtgtgg
    56581 tagatggtgc catgcagatt attggccgcc tgaaacagcg ggtgcatctg gcggacggcc
    56641 tggggccaga taatatgttg agtgaagatg caatgacgcg cggtttgaac tgtctgtcgc
    56701 tgtttgccga acggctacaa gggttttctc ctgccagcgt ctgtatagtc ggcacccata
    56761 cgctgcgtca ggcgctgaac gccactgact ttctgaaacg cgcggaaaag gtcattccct
    56821 acccgattga aattatttcc ggtaatgaag aagcccgtct gatttttatg ggcgtggaac
    56881 atacccaacc ggaaaaaggt cgcaaactgg ttattgatat tggcggcgga tctacggaac
    56941 tggtgattgg tgaaaatttc gaacctattc tcgttgaaag ccgccggatg ggttgtgtca
    57001 gctttgccca gctttatttt cctggcgggg tcatcaataa agagaatttt cagcgcgctc
    57061 gcatggcggc agcacaaaaa ctggaaactt taacctggca attccgtatt cagggctgga
    57121 acgttgcaat gggcgcttcc ggtaccataa aagccgccca tgaagtgtta atggaaatgg
    57181 gcgagaaaga cgggataatt accccggaac gtctggaaaa actggtaaaa gaagttttac
    57241 gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc cgaagagcgg aaaacagtct
    57301 tcgttcctgg actggcgatt ttatgtggtg tgtttgatgc tttagccatc cgtgaactgc
    57361 gcctttctga cggggcgtta cgcgaaggcg tactgtatga aatggaagga cgtttccgtc
    57421 atcaggatgt gcgtagtcgc accgccagca gcctcgccaa ccagtatcac atagacagcg
    57481 aacaggcccg acgagtgctg gataccacta tgcaaatgta cgaacagtgg cgggaacagc
    57541 aaccgaagct ggcgcatccg caactggagg cgctactgcg atgggccgcc atgctgcatg
    57601 aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca ctccgcttat attctgcaaa
    57661 acagtgactt gccgggtttt aatcaggaac agcagctgat gatggcgaca ctggtgcgct
    57721 atcaccgtaa agcgattaag ctcgacgatc tgccgcgctt taccttgttt aagaagaaac
    57781 agttcctgcc actgatacag ctattgcgcc ttggcgtatt actaaacaat caacgtcagg
    57841 caaccaccac accgccaaca ttgacactga ttactgatga cagtcactgg acactgcgtt
    57901 tcccgcatga ctggtttagt cagaatgcgc tggtactgct tgatctggaa aaggagcaag
    57961 aatactggga aggcgtggct ggctggcggt tgaaaattga agaagaaagt accccagaaa
    58021 tcgccgctta atgctttgcg ggccgacgag aaatgtcggc ccgcattatt caggcacttt
    58081 cgcgaatggg ttcgatttca ttcagcgtat caattaacgg ttgcggctta ccaataagat
    58141 aaccctgcat ataatcgatc cccaaagaga gcaccgcctc gcggatctct tcgttttcaa
    58201 cgtactctgc cactacccgc attttcttca ttcgcgccag atggcaaatc gacgccacaa
    58261 tctgataatc cagactattt gacacaatat tgcggataaa gctgccgtca attttaagca
    58321 gatcggcatt cacattttta agccgtgcat agctggcgta gccggtgccg aaatcatcaa
    58381 tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt gatttgcgcc tgcttaacat
    58441 tggtcagagc attactttcg gtgacttcaa aaataagttg ccacgcttca atctgatatt
    58501 tagccagcaa ctgactgact tcaacaggaa aacgagcctg acataccgag gttggagaca
    58561 gattaatagc aaaacggtga gcgggcatct tcgctctgtt ttcagccata aattgcagcg
    58621 tatgctcaat gacccacatg tcgatactcg acgataaacc aaattcgtgc gcgaccggca
    58681 agaagctatc ggggccgatc agttcatcat tctcaccttt catgcgcaga agaatttcat
    58741 ggtaaacatc accacgcata ccggtaatcg gctgggccat caggaaaaaa tggttgtgtt
    58801 ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc tttcaattcg cgttgcaaat
    58861 acattgcccc gcaacgctgc atattttccg gggcgttggt cacgatggaa agttcggcga
    58921 ccgtatttag ctctcccagc agcaggtaga tatgattcac tggcgagcgc acatagcagt
    58981 aactgacgcc aatctgcggt tgcatcggca tcccatccca aaagaaacga aattgcttga
    59041 gatgactatc cagtgcggta atgcgctcct ggtgcgattc tgtattcagt cgcagcgcga
    59101 gatcgttacc cgaaagctga taaacatctt cacccggttc cagcaagggt gacagccagt
    59161 gagaaagttt ttgcttgtat tgaatccgca gcatgatgcc atagttctta accagcattt
    59221 ccatgccaag gatgcgtaaa taacaaagcg cagaccaagg ggcatcacgc aacgcgcgat
    59281 tcagggcgcg aacatttggc agatgaacca ccgggtccac atacgcaagc cgctgaatgc
    59341 gtctgactac cgctcgctga cgagttgcca gtacagccat ataattgaca ataaaagaga
    59401 ataccagata actggaggag gttatggtca gctgcgtggt atagccagga taaatgggaa
    59461 tgtaattttg atagctgtgg atgctgatca tcaacacgac cgcccagagc agcgaaatca
    59521 gcttataacc atagcgcatt gctccccaca tcatcagggg cagcaataat gacaaggtat
    59581 aattggtgct aaaaattgtg cttttttcat ttaacggcat gcataacagc aacaataaag
    59641 cacctaatgc cagtagccag agcgcgaact cttttttggt gactttggca tcaacctgct
    59701 gttttaattg cgaataatag ctacgtaaat aaaatggatt tcgcactacc cgaatgatga
    59761 agtagcacag cgggacaccg atcagattac ccaccagtaa ggcctgataa ttgattaaag
    59821 tcccgaggtt aaaaggcatg acaccgacca gattttctct gctcgccagt aatcctacaa
    59881 atgcagcaaa ctggaaaagt atcagaaaca gcgttgcagg aaacactatc tgccagaata
    59941 tacgctggga aattaaacgg gtatcaccat gtgaaacgtt gttacgccgg ggagtaaaga
    60001 ccctgtaacc gccccaacag agaatgataa tgataataaa attagccgtt aatgagagcg
    60061 tttcgtaaaa acccagctct gcatatttgc ggacaaaaat ccctaaggtg atccctggca
    60121 atgccgccca gctgaaaaac atcatcatgc tgatcatcag tgccaaaggc agataaaaga
    60181 gaaaaacctc accggaagaa atatgcgcga aagtattaat gtgggcgaaa atgggcaaga
    60241 ttaaagaagg caggaacagc ggaagccccc accatttatc acgtattttt atataagttg
    60301 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
    60361 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
    60421 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
    60481 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
    60541 tccataacat taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt
    60601 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
    60661 ttttgtgagt caggcaacta gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
    60721 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
    60781 tgcctggcag caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
    60841 accggtacaa cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg
    60901 tcggatgctt ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt
    60961 acgtctttgt cctgactgat gtttatcctg tttggctgcg aaataaatat aaaattaata
    61021 tatatgttgt aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa
    61081 tctatccctt tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt
    61141 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
    61201 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
    61261 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
    61321 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
    61381 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
    61441 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
    61501 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag
//
LOCUS       DADKXM010000010        61355 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.010, whole genome shotgun sequence.
ACCESSION   DADKXM010000010 DADKXM010000000
VERSION     DADKXM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.010"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..795)
                     /locus_tag="JQR05_000611"
                     /pseudo
     CDS             complement(1..795)
                     /locus_tag="JQR05_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
     gene            977..2401
                     /gene="sbcB"
                     /locus_tag="JQR05_000612"
     CDS             977..2401
                     /gene="sbcB"
                     /locus_tag="JQR05_000612"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY5322849.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2444..2671)
                     /locus_tag="JQR05_000613"
     CDS             complement(2444..2671)
                     /locus_tag="JQR05_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY5322850.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2685..3743)
                     /gene="yeeE"
                     /locus_tag="JQR05_000614"
     CDS             complement(2685..3743)
                     /gene="yeeE"
                     /locus_tag="JQR05_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY5322851.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3922..5280)
                     /gene="plaP"
                     /locus_tag="JQR05_000615"
     CDS             complement(3922..5280)
                     /gene="plaP"
                     /locus_tag="JQR05_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY5322852.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5270..5332)
                     /gene="yoeI"
                     /locus_tag="JQR05_000616"
     CDS             complement(5270..5332)
                     /gene="yoeI"
                     /locus_tag="JQR05_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY5322853.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5547..6476)
                     /locus_tag="JQR05_000617"
     CDS             complement(5547..6476)
                     /locus_tag="JQR05_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5322854.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(6522..7346)
                     /locus_tag="JQR05_000618"
     CDS             complement(6522..7346)
                     /locus_tag="JQR05_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5322855.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGRIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7429..7683)
                     /gene="yoeB"
                     /locus_tag="JQR05_000619"
     CDS             complement(7429..7683)
                     /gene="yoeB"
                     /locus_tag="JQR05_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY5322856.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(7680..7931)
                     /gene="yefM"
                     /locus_tag="JQR05_000620"
     CDS             complement(7680..7931)
                     /gene="yefM"
                     /locus_tag="JQR05_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY5322857.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            8214..8264
                     /gene="hisL"
                     /locus_tag="JQR05_000621"
     CDS             8214..8264
                     /gene="hisL"
                     /locus_tag="JQR05_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY5322858.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            8410..9309
                     /gene="hisG"
                     /locus_tag="JQR05_000622"
     CDS             8410..9309
                     /gene="hisG"
                     /locus_tag="JQR05_000622"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY5322859.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            9315..10619
                     /gene="hisD"
                     /locus_tag="JQR05_000623"
     CDS             9315..10619
                     /gene="hisD"
                     /locus_tag="JQR05_000623"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY5322860.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYVAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            10616..11686
                     /gene="hisC"
                     /locus_tag="JQR05_000624"
     CDS             10616..11686
                     /gene="hisC"
                     /locus_tag="JQR05_000624"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY5322861.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRPLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            11686..12753
                     /gene="hisB"
                     /locus_tag="JQR05_000625"
     CDS             11686..12753
                     /gene="hisB"
                     /locus_tag="JQR05_000625"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY5322862.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFS
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            12753..13343
                     /gene="hisH"
                     /locus_tag="JQR05_000626"
     CDS             12753..13343
                     /gene="hisH"
                     /locus_tag="JQR05_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY5322863.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRHSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            13343..14080
                     /gene="hisA"
                     /locus_tag="JQR05_000627"
     CDS             13343..14080
                     /gene="hisA"
                     /locus_tag="JQR05_000627"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY5322864.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            14062..14838
                     /gene="hisF"
                     /locus_tag="JQR05_000628"
     CDS             14062..14838
                     /gene="hisF"
                     /locus_tag="JQR05_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY5322865.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            14832..15443
                     /locus_tag="JQR05_000629"
     CDS             14832..15443
                     /locus_tag="JQR05_000629"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY5322866.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANSIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPEISYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(15629..16606)
                     /gene="wzzB"
                     /locus_tag="JQR05_000630"
     CDS             complement(15629..16606)
                     /gene="wzzB"
                     /locus_tag="JQR05_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY5322867.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(16752..17918)
                     /gene="ugd"
                     /locus_tag="JQR05_000631"
     CDS             complement(16752..17918)
                     /gene="ugd"
                     /locus_tag="JQR05_000631"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY5322868.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(18168..19574)
                     /gene="gndA"
                     /locus_tag="JQR05_000632"
     CDS             complement(18168..19574)
                     /gene="gndA"
                     /locus_tag="JQR05_000632"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY5322869.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(19683..20042)
                     /locus_tag="JQR05_000633"
     CDS             complement(19683..20042)
                     /locus_tag="JQR05_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322870.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(20039..20591)
                     /locus_tag="JQR05_000634"
                     /pseudo
     CDS             complement(20039..20591)
                     /locus_tag="JQR05_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20692..21291)
                     /locus_tag="JQR05_000635"
     CDS             complement(20692..21291)
                     /locus_tag="JQR05_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322871.1"
                     /translation="MSLSILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFAVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYLGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(21476..22402)
                     /locus_tag="JQR05_000636"
                     /pseudo
     CDS             complement(21476..22402)
                     /locus_tag="JQR05_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(22465..22839)
                     /locus_tag="JQR05_000637"
     CDS             complement(22465..22839)
                     /locus_tag="JQR05_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY5322872.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(22843..23991)
                     /gene="wzy"
                     /locus_tag="JQR05_000638"
     CDS             complement(22843..23991)
                     /gene="wzy"
                     /locus_tag="JQR05_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY5322873.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEVLQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(24011..24880)
                     /locus_tag="JQR05_000639"
     CDS             complement(24011..24880)
                     /locus_tag="JQR05_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY5322874.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(24909..25799)
                     /locus_tag="JQR05_000640"
     CDS             complement(24909..25799)
                     /locus_tag="JQR05_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY5322875.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGDGRYNA"
     gene            complement(25829..27085)
                     /gene="wzx"
                     /locus_tag="JQR05_000641"
     CDS             complement(25829..27085)
                     /gene="wzx"
                     /locus_tag="JQR05_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY5322876.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(27101..27646)
                     /gene="rfbC"
                     /locus_tag="JQR05_000642"
     CDS             complement(27101..27646)
                     /gene="rfbC"
                     /locus_tag="JQR05_000642"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY5322877.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWQVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(27651..28529)
                     /gene="rfbA"
                     /locus_tag="JQR05_000643"
     CDS             complement(27651..28529)
                     /gene="rfbA"
                     /locus_tag="JQR05_000643"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY5322878.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAGQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(28588..29487)
                     /gene="rfbD"
                     /locus_tag="JQR05_000644"
     CDS             complement(28588..29487)
                     /gene="rfbD"
                     /locus_tag="JQR05_000644"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY5322879.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(29487..30572)
                     /gene="rfbB"
                     /locus_tag="JQR05_000645"
     CDS             complement(29487..30572)
                     /gene="rfbB"
                     /locus_tag="JQR05_000645"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY5322880.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(30945..31838)
                     /gene="galF"
                     /locus_tag="JQR05_000646"
     CDS             complement(30945..31838)
                     /gene="galF"
                     /locus_tag="JQR05_000646"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY5322881.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(32013..33407)
                     /gene="wcaM"
                     /locus_tag="JQR05_000647"
                     /pseudo
     CDS             complement(32013..33407)
                     /gene="wcaM"
                     /locus_tag="JQR05_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
     gene            complement(33418..34638)
                     /gene="wcaL"
                     /locus_tag="JQR05_000648"
     CDS             complement(33418..34638)
                     /gene="wcaL"
                     /locus_tag="JQR05_000648"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY5322882.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(34635..35915)
                     /gene="wcaK"
                     /locus_tag="JQR05_000649"
     CDS             complement(34635..35915)
                     /gene="wcaK"
                     /locus_tag="JQR05_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY5322883.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIEEVK"
     gene            complement(36095..37573)
                     /gene="wzxC"
                     /locus_tag="JQR05_000650"
     CDS             complement(36095..37573)
                     /gene="wzxC"
                     /locus_tag="JQR05_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY5322884.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIIHYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(37575..38969)
                     /gene="wcaJ"
                     /locus_tag="JQR05_000651"
     CDS             complement(37575..38969)
                     /gene="wcaJ"
                     /locus_tag="JQR05_000651"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY5322885.1"
                     /translation="MINLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(39024..40394)
                     /gene="cpsG"
                     /locus_tag="JQR05_000652"
     CDS             complement(39024..40394)
                     /gene="cpsG"
                     /locus_tag="JQR05_000652"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY5322886.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(40587..42023)
                     /gene="cpsB"
                     /locus_tag="JQR05_000653"
     CDS             complement(40587..42023)
                     /gene="cpsB"
                     /locus_tag="JQR05_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY5322887.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETTQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(42026..43249)
                     /gene="wcaI"
                     /locus_tag="JQR05_000654"
     CDS             complement(42026..43249)
                     /gene="wcaI"
                     /locus_tag="JQR05_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY5322888.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(43246..43725)
                     /locus_tag="JQR05_000655"
     CDS             complement(43246..43725)
                     /locus_tag="JQR05_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY5322889.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(43725..44693)
                     /gene="fcl"
                     /locus_tag="JQR05_000656"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(43725..44693)
                     /gene="fcl"
                     /locus_tag="JQR05_000656"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY5322890.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPASLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(44696..45817)
                     /gene="gmd"
                     /locus_tag="JQR05_000657"
     CDS             complement(44696..45817)
                     /gene="gmd"
                     /locus_tag="JQR05_000657"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY5322891.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(45843..46391)
                     /gene="wcaF"
                     /locus_tag="JQR05_000658"
     CDS             complement(45843..46391)
                     /gene="wcaF"
                     /locus_tag="JQR05_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY5322892.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVALGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(46407..47153)
                     /gene="wcaE"
                     /locus_tag="JQR05_000659"
     CDS             complement(46407..47153)
                     /gene="wcaE"
                     /locus_tag="JQR05_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY5322893.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLVHLAQADDISFEWIVVDGGSN
                     DATREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            complement(47164..48381)
                     /gene="wcaD"
                     /locus_tag="JQR05_000660"
     CDS             complement(47164..48381)
                     /gene="wcaD"
                     /locus_tag="JQR05_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY5322894.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMEPISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEVIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFSDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(48356..49573)
                     /gene="wcaC"
                     /locus_tag="JQR05_000661"
     CDS             complement(48356..49573)
                     /gene="wcaC"
                     /locus_tag="JQR05_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY5322895.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(49570..50058)
                     /gene="wcaB"
                     /locus_tag="JQR05_000662"
     CDS             complement(49570..50058)
                     /gene="wcaB"
                     /locus_tag="JQR05_000662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY5322896.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(50061..50900)
                     /gene="wcaA"
                     /locus_tag="JQR05_000663"
     CDS             complement(50061..50900)
                     /gene="wcaA"
                     /locus_tag="JQR05_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY5322897.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(50992..53154)
                     /gene="wzc"
                     /locus_tag="JQR05_000664"
     CDS             complement(50992..53154)
                     /gene="wzc"
                     /locus_tag="JQR05_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY5322898.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHTRPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGGSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(53157..53600)
                     /gene="wzb"
                     /locus_tag="JQR05_000665"
     CDS             complement(53157..53600)
                     /gene="wzb"
                     /locus_tag="JQR05_000665"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY5322899.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(53606..54745)
                     /locus_tag="JQR05_000666"
     CDS             complement(53606..54745)
                     /locus_tag="JQR05_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY5322900.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            55404..56987
                     /locus_tag="JQR05_000667"
     CDS             55404..56987
                     /locus_tag="JQR05_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY5322901.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFSFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(57439..59292)
                     /gene="asmA"
                     /locus_tag="JQR05_000668"
     CDS             complement(57439..59292)
                     /gene="asmA"
                     /locus_tag="JQR05_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY5322902.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(59314..59895)
                     /gene="dcd"
                     /locus_tag="JQR05_000669"
     CDS             complement(59314..59895)
                     /gene="dcd"
                     /locus_tag="JQR05_000669"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY5322903.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(59987..60628)
                     /gene="udk"
                     /locus_tag="JQR05_000670"
     CDS             complement(59987..60628)
                     /gene="udk"
                     /locus_tag="JQR05_000670"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY5322904.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            60946..>61355
                     /locus_tag="JQR05_000671"
     CDS             60946..>61355
                     /locus_tag="JQR05_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322905.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTCSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT        15925 a        15622 c        14249 g        15559 t
ORIGIN      
        1 gctatgctct ttcggtacgg tccatacgtt gtccagcagg tcaaattctg tctttgttgc
       61 cagcctaagc tctgagagcc tcgcccccca cagcataagc atctgatgaa ggagcttatt
      121 tgacgtagac gcacggcttc tttcaatagc aagccaaatc ttagccagtt cgtgatacga
      181 cagtacccgc tcccctacct cagcgcggga gccgaagtcc cttggctgga tgctcataat
      241 tgcgcaacta tctatcaact gacgccgcat gcaccaacta attgctgatc ttagttgact
      301 tagcacctgc cttgctcggc gtggattatc tttttcttct tcggtaagca ggtcaaccta
      361 ttgcttaacc gtgatagaag atgccggacg attagggaag gcgtcatgca tgcgcttcat
      421 aaccgttgat cgataaagtg cctgggtctt ttctctgaga gttgtagaga cgtagttgtc
      481 gaaccagtag tcgagacact gggcgaccgt catggagttc tccaccttct cttcaaaata
      541 ggtgcgtgga tccgttcctg agaaatagag ctttcgcaag tcagcagtga tctgtctggc
      601 atccttcaaa gacagggatg ggtatcgacc aagcccaagt cgattaggct tgccatgcca
      661 gcgatagcgg tactggaact ggatgacccc cttcggtgaa attcgtacgc tgaggccatc
      721 ggcatcagcc acttcttgtg ggcccgaata tggtttacca taaatagtac gcagttttgt
      781 gtcgcttata gccataaata atatttggta cgctcagaaa taatagtttg gtacacatct
      841 ggtacacaat atcacatgga caacagcgca cagtaaacaa ctatatttga cgttcgtaga
      901 catactcagg tgaaaaaagg gttgtttttc gagttataac agacagttaa ttaactacag
      961 tggacaatgc tagacaatga cagacacaga taaacaacct accttcctct ttcacgatta
     1021 cgaaaccttt ggcacgcacc ccgcgttaga tcgccctgca cagttcgcag ccattcgcac
     1081 cgatgacgaa ttcaatgtca tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga
     1141 ctatttaccc cggcctggag cagtattaat taccggtatt accccgcagg aggcacgggc
     1201 gaaaggagaa aacgaagccg cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa
     1261 gacctgtatt ctgggctaca acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt
     1321 ttatcgtaat ttctacgatc cttacgcctg gagctggcag catgataact cgcgctggga
     1381 tttactggat gttatgcgtg cctgttatgc tctgcgcccg gaaggaataa actggcctga
     1441 aaatgatgac ggtctaccga gctttcgcct tgagcattta accaaagcga atggtattga
     1501 acatagcaac gcccacgatg cgatggctga tgtgtacgcc actattgcga tggcgaaact
     1561 ggtaaaaacg cgtcagccac gcctgtttga ttatctcttt acccatcgta ataaacacaa
     1621 actgatggcg ttgattgatg ttccgcagat gaaacccctg gctcacgttt ccggaatgtt
     1681 tggggcatgg cgtggcaata ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg
     1741 caatgccgta attatggcgg atttggcagg agacatttcg ccattactgg agctggatat
     1801 cgacacattg cgcgagcgtt tatataccgc aaaagccgat cttggcgata acgccgccgt
     1861 tccggttaag ctggtgcata tcaataaatg tccggtactg gcccaggcga atacgctacg
     1921 cccggaagat gccgaccgac tgggaattaa tcgtcagcat tgcctcgata acctaaaaat
     1981 tctgcgtgaa aatccgcaag tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc
     2041 gtttacacct tcagataatg tggatgcaca gctttataat ggctttttca gtgacgccga
     2101 tcgtgcagca atgaaaattg tgctggaaac cgagccgcgt aatttaccgg cactggatat
     2161 cacttttgtt gataaacgaa ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc
     2221 ggggacgctg gattatgccg agcagcaacg ctggctggag caccgccgcc aggtcttcac
     2281 gccagagttt ttgcagggtt atgctgatga attgcagatg ctggtacaac aatatgccga
     2341 tgacaaagag aaagtggcgc tgttaaaagc actttggcag tacgcggaag agatcgtcta
     2401 atttgaagcc gccgccgctg gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg
     2461 tgatgctcca cgctgcatcg ccaatctgct gataatcggt gatggcatgt ccttcttctg
     2521 ccgcccactg tgggattgct tcagttgcct gggtgcaatc aaactcaatt accaactcat
     2581 cgccgctggc catttctgcc agtgctgctt ttgcttctat gagtggaaac ggacagacct
     2641 gcgtcaccac atccagcttt ttaatcgcca tctttacacc ttacttaatt tgccgcagca
     2701 gttgccagtc gcgccttacg ctgcggtcga acatacacaa gccaggacgc agtccagact
     2761 ccgagaatca taaatacaag gccaatccag ccctgccagg tcatcattgc agtcataacc
     2821 agtccattac cgatagagca accacctgca atactggcac cgaagcccat cagtacacca
     2881 cctaacccgc tacgtaatgt tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc
     2941 gctttggctg caataaatgc ccccacgaag atccctaaca ccaggaaaac gccccagtta
     3001 atgtatttca cgtcacccgc gaccagaaat tgcagaatat tggccgttgg ggaagtgact
     3061 cctaacccaa acatgcgtcc ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa
     3121 ccgatgagta cagcagtgac aaagggatgc cagcgttttt caaacagaat atgagcgatc
     3181 ccggttcggc gcggcggtaa ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc
     3241 accacccaga gcgttatcac cagcaaaacg gtaaccaacg gccacacaga caaattaaaa
     3301 gtctcagcaa tagagttatg ttcagtagtg tagtgctgca aggtttgatt taaaccactg
     3361 gcgtgtggag aacgcatcac cgcactcatc accatataag tgaaaagcgc gatccaactg
     3421 ccgatcaatc cctcacccgc gcgataccag gtcccggtgg cacatccacc cgccagaaca
     3481 attcccagcc cgaaaagata cccacctata acagtaccaa gccacgggaa cgccccggct
     3541 tcgtaagtca gtaggcccgc ctgaattaac gcaaaaaccc ccacgctttg taccgaaatt
     3601 gcaatcagca aggcgtaaaa catgcgatta tttttcgcga tatacatatc acgaaaacca
     3661 ccagtcaggc aaaaacgccc acgctgcatg acaaatccca gcagagcacc acaaattagc
     3721 ccgcttaata tcattgaaaa cataaattaa ttaaccagat gaatgttaat ggggaaattt
     3781 ttcatgactg gtggaatgca gaccaataac caaattctct aaattagaac aaatggttat
     3841 taatgaggat ttcgataaaa aagaataaaa aaaccggagc caaatgttca ttcgactcct
     3901 gtttattatt agaaaagatg attatgctac gtcttcgtac tgcggaaccg gattacggaa
     3961 gctcttggtg acgcaagcca ggtaaatcag accgatagca gcccagatca gacccagaac
     4021 cattgagctt tcttccaggt taacccacag cgcaccaacg gtcagcgcac cacacatcgg
     4081 caggaacaga tactggaagt gatctttcag cgtcttgtta cgcttctcac ggatccagaa
     4141 ctgcgagatg accgacaggt taacaaaggt gaacgccacc agcgcaccaa agttaatcag
     4201 cgccgttgcc attaccaggt cgaagttgat tgccagcagg gcaatcgcgc caaccaggat
     4261 gatgttcatc gccggagtac gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa
     4321 cacgccgtca cgacccatta cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc
     4381 ggacgccagt acggtaatgg tggagaagat cagcgcgcca acctggaacg ctttacccgc
     4441 aacgtacagc atgatttcag gctgtgacgc atccggatct ttaaagcgag agatatccgg
     4501 gaagtacagc tgcaggaagt aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt
     4561 caggaaaatc gcacgcggga tcacgcgctc tgcatctttg gtttcttccg acagattact
     4621 gatgccgtca aagccggtaa aggagaagca caggattgtc gccccggtaa tcatcgggat
     4681 aacatgcgca tcgccagacc agaacggacg cgtgctcgcc agcgtaccag cgccttcacc
     4741 ttcaaatacg ccataaacaa ccatgcccag aatcaccgcg atcagcacta cctgcaacac
     4801 gacgattaca gtgttgaagt tcgctacgga tttcagacta cgcaggttaa aggcggtcat
     4861 aaaggccacc agcgccacca caaacatcca cgatggaatg gaaggcacca gagcttcaaa
     4921 atagattttc gccagcagaa tgttgatcat cggcgcgaac agatagtcga gcagagaaga
     4981 ccaacccacc ataaagccga cagtcgggct aatggatttc tgggcgtaag tgtatgcaga
     5041 gccagcagaa ggatagcggc gaaccagctt cccgtagctc agagccgtaa acaggatcgc
     5101 aatcaatgcg aacgcatagg ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc
     5161 aaatgtatca aacagcgtca tcggctgcat ataggctaga cccatcatta caaccggaac
     5221 taacgtaagc gttttacgca attccacgcg agaggtgttt ggagtaacgt tatgcgacat
     5281 ggttattctc ctttacggtg ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg
     5341 attcctcagc gacaacaact gtcgattttt agtaaatatc tatccggtac gaagcccggc
     5401 ctcttggtat gaatgattgg tttgaagcaa aaaataaccg acgctaatta aacgtcggtt
     5461 tttagtcatt ttttgacagc ggcgcattgt gcctaaatgg gggggaaatg acaagagaat
     5521 gagaggcttg tcataataat ttttctttaa atggctgatt ttccgtcatc agattttgca
     5581 taaacaccgg cgatcgcact atttgctaaa atttcatctc gccaccagcc gctggcaagc
     5641 cctgcggtct gttcattcca gccaatccag acaggttcaa agagagtttg ggcgaccact
     5701 ttcttctcga ttaacgcgcc actatcgaga aaacgttgcg ccagataccg tggtaaatag
     5761 ccgcagccaa ggccgctaat ttgcagctcc agtttggttt taaaatcgaa aacggtaatg
     5821 gcttcttgtt cgtcgagaag ttgtgaggcc gtagaagcgc ctgcctgcgc agtgtccccc
     5881 accacaatcg cccgatatcg tttaatgata cgacggttta acggttcttc ttcctgggct
     5941 aacggatgat gaggcgcgac cgcaaagact tgttcaagat cgcccaaccg cgagaaacca
     6001 aactcactgg aagatggtgg ctcatgcatc gcgccaacga taatatccgc tcgcccctga
     6061 gtcagggcat cccaggaacc accgagtacg ccattgataa acttcaggcg cgtcacgcta
     6121 tgatgttgat aaaaagcttc aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc
     6181 acgccaatca ccagctcgtt ttcccagcct tcatgcagtt tgatcgcctg cttttccagt
     6241 tctcgcacgg tatgcagaac ttcccgccct ttctctaata acatttttcc ggtgcgggtg
     6301 aatttagcgc ggtgtccgct acgatcaagc aattgaatat ttaggtcgct ttccagcttg
     6361 tgaacggtgt aactcagagc ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa
     6421 ctgccctctt tttctatggc atcgaggatc atcaacacat ccagcagtgg tttcatacac
     6481 gcccccttgc agtgcatgcg gcgtcctgct ggcgtgatcg tttactccag cggcattacc
     6541 agcggatcgg gatactggta ttcaaatccc agttcattac aaatccgact gccatcaata
     6601 atcctacctt tgccgctgtc cagactattt ctgaactgcg gaggttgcag ccccagtaaa
     6661 cgggccatct gcggatagaa aacattacgc gcagggtgag ctggcgcaca tatattatag
     6721 atgtgtccgc ctttaggtgc ctgtaacaac agagtgatag cgccaataac atcttctaaa
     6781 tggactaaat taacaccatg ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc
     6841 ggatgacgtc ccggtccgac caggcccgca agacgcagaa tatcgaccga agtaccgggt
     6901 aaattgtgca gccagtcttc gagttcttct aatactcgtc cactgttggt taccggatta
     6961 cgcggggtag tttctttaac cgtgccttgc gcgtcgccat agacagatgt tgagctggta
     7021 aaaataattc ggggaatacg atgggccagc gcgctatcca ctaactcttg taccgcttgt
     7081 aaatagaact catcgccggg gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg
     7141 gcatccataa gggcatccag atcgtcagaa tcgcaaacta actcaggctc catgcgaagc
     7201 agatagctat caatgccact cattcgggcc gcttcgacac catcttgtgt ggttttactc
     7261 ccggtgactt gccagcctcg cgctgaaagt gacatcgcca gcggcatacc taaccaccct
     7321 aacccgacaa ttgcgacctt tttcatccgt tatctcctga tacaccttag atctataagg
     7381 ctacgctagc gtatcaaaac tgacaattca ttctatgaat gaatctgttc aataatgata
     7441 acgacatgct gcaatgagca gtgaatcatc agtaaccgcg tataccagac ggtgctcctc
     7501 tgtaatgcgt cgggaccaga aacctgacaa attatgtttc aggggttctg gtttcccctt
     7561 accttcaaat ggtgttctgc gggtatcttt gataagttca ttgatctttt taacaattcg
     7621 cttatctgtt tcctgccagt acagataatc atcccatgat tcctcagacc agattagttt
     7681 cactcaataa tgtccttttc cgttcctttg cctgatttca ggctatcgat tgagtccatc
     7741 aatctccggg cgttagcggg ggaacgcagt agataagccg tctcttccag cgagttgtat
     7801 tcttcgagtg acatcagaac acaagcctct ccattctgac gagtaatgag gatcggggca
     7861 tgatcttcaa cggctttcat cattgttgcc gacaaattct gacgcgcttc gctgtagcta
     7921 attgtacgca tgtaaatctc ctattttgta cagttcattg tacaatgata ggtgttaatt
     7981 aactatttat taattagttt gtagatcaag acattgccag cgagacgaaa aatcagactt
     8041 tgctcttttt ggtgctgtca agttagagga cagtcctctt agccccctcc tttccccgct
     8101 cattcattaa acaaatccat cgccataaaa tatataaaaa agcccttgct ttctaacgtg
     8161 aaagtggttt aggttaaaag acatcagttg aataaacatt cacagagatt tttatgacac
     8221 gcgttcaatt taaacaccac catcatcacc atcatcctga ctagtctttc aggcgatgtg
     8281 tgctggaaga cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga
     8341 tcaccttccg ggggcttttt tattgcgcgg ttgttaacgg ttcagacagg tttaaagagg
     8401 aataagaaaa tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta
     8461 agtgatgact cacgcgaatt actggcgcgc tgtggcatta aaatcaatct tcacacccag
     8521 cgcctgatcg cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgatgac
     8581 attcccggtc tggtaatgga cggcgtggta gaccttggga ttatcggcga aaacgtgctg
     8641 gaagaagagc tgcttaaccg ccgcgcccag ggtgaagatc cgcgctactt taccctgcgt
     8701 cgtctggatt tcggcggctg ccgcctttcg ctggcaacgc cggttgatga agcctgggac
     8761 ggtccgctct ccttaaacgg taaacgtatc gccacctctt atcctcacct gctcaagcgt
     8821 tatctcgacc agaaaggtat ctcttttaaa tcctgcttac tgaacggttc tgtcgaagtt
     8881 gcgccgcgtg ccgggctggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg
     8941 gaagctaacg gcctgcgcga agtcgaagtt atctaccgct cgaaagcctg cctgatccag
     9001 cgcgatggcg aaatggaaga atctaaacag caactgatcg acaagctact gacccgtatt
     9061 cagggtgtga tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgc
     9121 ctggatgaag tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg
     9181 ggtgatcaac agcgcgtagc gatgcacatg gtgagtagcg aaaccctgtt ctgggaaacg
     9241 atggaaaaac tgaaagcact gggtgccagt tcaatcctgg tcctgccgat tgagaagatg
     9301 atggagtgat cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc
     9361 aacaacgcca gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcaccg
     9421 ttaacgatat tctcgataac gtgaaaacgc gtggcgatga ggccctgcgt gaatacagcg
     9481 cgaagtttga taaaaccacg gttaccgcgc tgaaggtgtc tgctgatgag atcgccgccg
     9541 ccagcgaacg cctgagcgac gagctaaaac aggcgatggc ggtggcagta aagagtattg
     9601 aaaccttcca cactgcgcaa aaactgccgc cggtagatgt agaaacgcag ccaggcgtgc
     9661 gttgccagca ggtcacgcgc ccggtggctt cagtgggttt gtatattccg ggcggctccg
     9721 ccccgctctt ctcaacggta ttaatgctgg caactccggc gcgtattgcg ggctgtaaaa
     9781 aagtggtgct gtgctcaccg ccgccgattg ccgatgaaat tctttatgtg gcgcagctgt
     9841 gcgatgtgca ggacgtgttt aacgtcggcg gcgcacaggc cattgccgcg ctggcgtttg
     9901 gtacggaatc tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg
     9961 aagcaaaacg ccaggtaagc cagcgtctgg acggtgcggc gatcgatatg cccgcaggcc
    10021 cgtcggaagt gctggtgatt gctgacagcg gcgctacgcc ggattttgtg gcttctgatt
    10081 tgctttctca ggctgaacac ggccctgact cacaggtaat tttactgacc cccgatgctg
    10141 atatggcgcg tcgcgttgcc gaggccgtcg aacgccaact ggcagaactg ccgcgtgccg
    10201 aaaccacccg ccaggcactg aacgccagcc gcctgatcgt gactaaagat ttagcgcagt
    10261 gcgtggagat ctccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgccc
    10321 gcgaactggt cgatggcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg
    10381 aatcggcagg cgactacgcc tccggcacca accacgtgct gccgacttac ggttacaccg
    10441 ccacctgttc cagcctcggg ctggcagatt tccagaaacg catgaccgta caggaactgt
    10501 cgaaagaagg tttctccgcg ctggcttcga ccattgaaac actggccgcc gccgagcgcc
    10561 tgaccgccca caaaaatgcc gttactttgc gtgttaacgt ccttaaggag caagcatgag
    10621 caccgtgact attactgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc
    10681 ggcgcgtcgt ctgggcggta acggcgacgt ctggctgaac gccaacgaat atcccacagc
    10741 cgtggagttt cagcttactc agcaaacgct caaccgctac ccggaatgcc agccaaaagc
    10801 ggtgattgaa aattacgcgc aatatgcagg cgtaaaaccg gaacaggtgc tggtcagccg
    10861 tggcgcggac gaaggtattg aactgctgat ccgcgctttt tgcgaaccgg gtaaagacgc
    10921 catcctctac tgcccgccaa cgtacggcat gtacagcgtc agcgctgaaa ccattggtgt
    10981 cgaatgccgc acagtgccga cgctggaaaa ctggcaactg gacttacagg gcatttccga
    11041 caagctggac ggcgtaaaag tggtctatgt ttgcagcccc aacaacccca ccgggcaact
    11101 gatcaatccg caggattttc gccccctgct ggagctaacc cgcggtaagg cgattgtggt
    11161 tgccgatgaa gcttatatcg agttttgccc gcaggcatcg ttagctggct ggctggcgga
    11221 atatccgcac ctggcgattt tgcgtaccct ttcgaaagcc ttcgcgttgg cggggcttcg
    11281 ttgcggattt acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcccccc
    11341 ctacccgctc tcgacgccgg ttgccgacat tgccgcacag gcgttaagcc cgcagggaat
    11401 cgtcgccatg cgcgaacggg tggcgcaaat tattacagaa cgcgaatacc tgattgccgc
    11461 actgaaagag atcccctgcg tggagcaggt tttcgactct gaaaccaact atattctggc
    11521 gcgctttaaa gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg
    11581 tgatcagaat aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga
    11641 agaaagccag cgcgtcattg acgccttacg tgcggagcaa gtttgatgag tcagaagtat
    11701 ctttttatcg atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac
    11761 cgttttgaca aactcgcctt tgaaccgggc gtgatcccgg aattgctgaa gctgcaaaaa
    11821 gcgggctaca agctggtgat gatcactaat caggatggtc tgggaacaca aagtttcccg
    11881 caagcggatt ttgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgtg
    11941 cagtttgatg aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag
    12001 ccgaaagtaa aactggtgga gggttatctg gctgagcaag cgatggatcg tgccaacagt
    12061 tatgtaattg gcgatcgcgc gaccgacatt caactggcgg aaaacatggg cattaatggt
    12121 ttacgctacg accgcgaaac cctgaactgg ccaatgattg gcgagcaact cactaaacga
    12181 gaccgttacg cccatgtagt gcgcaacacc aaagagacgc agattgacgt ccaggtgtgg
    12241 ctggatcgcg aaggtggcag caagattaac accggcgttg gcttctttga tcacatgctg
    12301 gatcagatcg ccacccacgg cggtttccgc atggaaatca atgtcaaagg cgacctctat
    12361 atcgacgatc accacaccgt cgaagatacc ggcctggcgc tgggcgaagc gttaaaaatt
    12421 gccctcggcg acaaacgcgg tatttgccgc tttggttttg tgctgccgat ggacgaatgc
    12481 cttgcccgct gcgcactgga tatctctggt cgcccgcacc tggaatataa agccgagttt
    12541 acctaccagc gtgtgggcga tctcagcact gaaatgatcg aacacttctt ccgttcgctc
    12601 tcttacacca tgggcgtgac actacacctg aaaaccaaag gtaaaaacga tcatcaccgt
    12661 gtagaaagcc tgttcaaagc ctttagtcgt accctgcgcc aggccatccg cgtcgaaggc
    12721 gacaccctgc cctcgtcgaa aggagtgctg taatgaacgt ggtgatcctt gatactggct
    12781 gcgccaacct gaactctgtg aagtctgcca ttgcgcgcca cggttatgaa cccaaagtca
    12841 gccgtgaccc ggacgttgtg ttgctggccg ataaactgtt tttacccggc gtcggcaccg
    12901 cacaagcagc gatggatcag gtgcgtgagc gcgagctgtt tgatctcatc aaagcctgta
    12961 cccaaccggt gctgggcatc tgcttaggga tgcaactcct gggacgacac agcgaagaga
    13021 gcaatggcgt cgacttgctg ggcatcatcg acgaagacgt gccgaaaatg accgactttg
    13081 gtctgccact gccacatatg ggctggaacc gcgtttatcc gcaggcaggc aaccgcctgt
    13141 ttcagggtat tgaagacggc gcgtactttt actttgttca cagctacgca atgccagtca
    13201 atccgtggac catcgcccag tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag
    13261 ataacttcta cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc
    13321 tgaaaaactt cctggagatg tgatgattat tccggcatta gatttaatcg acggcaccgt
    13381 ggtgcgtctc catcagggcg attacggcaa acaacgcgat tacggtaacg acccgctgcc
    13441 gcgcttacag gattacgctg cgcaaggtgc cgaagtgttg cacctggtgg atctgaccgg
    13501 ggcaaaagat ccggctaagc gtcaaatccc gctgattaaa accctggtcg cgggcgttaa
    13561 cgtcccggtg caggttggcg gcggtgttcg tagtgaagaa gacgtggcgg cgttactgga
    13621 agctggcgtt gcgcgcgtgg tggtcggttc caccgcagtg aaatcaccgg acgtagtgaa
    13681 aggctggttt gaacgtttcg gtgccgatgc gctggtgctg gcactggatg tccgtattga
    13741 cgagcaaggc aacaagcaag tggcagtcag cggctggcaa gagaactcgg gcgtttcact
    13801 ggaacaactg gtggaaacct atctgcccgt cggcctgaaa catgtgttgt gtaccgatat
    13861 ctcgcgcgac ggcacgctgg cgggatctaa cgtctcttta tatgaagaag tgtgtgccag
    13921 atatccgcaa gtggcgtttc agtcctccgg cggcattggc gacattaacg atgttgctgc
    13981 cctacgtggc actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt
    14041 caccgtgaag gaggccatcg catgctggca aaacgcataa tcccgtgtct cgacgttcgt
    14101 gatggtcagg tggtgaaagg cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg
    14161 ccactggcaa aacgctacgc cgaagaaggt gcagatgaac tggtgttcta cgatatcacc
    14221 gcttccagcg atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc
    14281 gacattccgt tttgtgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt
    14341 tcctttggcg cggataaaat ttccatcaac tcccctgcgc tggcagaccc aacattaatt
    14401 actcgcctgg ccgatcgctt tggcgtgcag tgtattgtgg ttggtattga tacctggtac
    14461 gacgccgaaa ccggtaaata tcatgtaaat caatataccg gcgatgaaag ccgcacccgc
    14521 gtcactcaat gggaaacgct cgactgggta gaggaggtgc aaaaacgcgg cgccggagaa
    14581 atcgtcctca acatgatgaa tcaggacggc gtgcgtaacg gttacgacct caagcagctg
    14641 aaaaaagtgc gtgaagtttg ccacgtcccg ctgattgcct ccggtggcgc gggcaccatg
    14701 gaacacttcc tggaagcctt ccgcgatgcc gacgttgacg gtgcgctggc agcttccgta
    14761 ttccacaaac aaataatcaa tattggcgaa ttaaaagcgt acctggcaac acagggcgtg
    14821 gagatcagga tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg
    14881 gtctgatgcc ggtgattgtg caacacgcgg tatctggcga agtgttaatg ctgggctaca
    14941 tgaacccgga agccttagac aaaaccatcg aaagcggtaa agtcaccttc ttctcgcgca
    15001 ctaaacagcg actgtggacc aaaggcgaaa cctcgggcaa ttttctcaat gtggtaagta
    15061 ttgccccgga ctgcgacaac gacacattac tggtgctggc gaattccatc gggccaacct
    15121 gccacaaagg caccagtagc tgcttcggca acaccgctca ccagtggctg ttcctgtatc
    15181 aactggaaca actgctcgcc gaacgtaaat atgccgaccc ggaaatctcc tacaccgcca
    15241 aactgtatgc cagcggcact aaacgcattg cgcagaaagt gggcgaagaa ggcgtggaaa
    15301 ccgcactggc agcaacggta catgatcgct ttgagctgac caacgaggca tctgacttga
    15361 tgtatcacct gctggtgctg ttgcaggatc aggatctgga tttaacgaca gtaattgaga
    15421 acctgcgtaa acggcatcag tgagttacgg gctgagcagg tgtgatgttg ttgtcacacc
    15481 cggagccatt gcccgatgcg caaacttatc gtgcctacac attatctcaa cacacgtagg
    15541 ccggataaga tgcgccagca tcgcatccgg caaaaaaaac gggcatggtg tcaccaccct
    15601 gcccgtttct cttaaatgca caataatatt acttcgcgtt gtaattacgc agagcgttac
    15661 gccccaacac aatccccgcg ccaaccatgc cgcccagaag cactgccaga atcaaggtga
    15721 ttgccttttt cggactatcg cgacgaatag gtaacgtcgg tttcatcaca tagcggtaag
    15781 catgaatatc aagatcatca aactttaatt tttcaatatc caacaggttt tgacgtgtct
    15841 gatagtagtt tggtgagaac accaacggac gagtcgcttc atgcttaatc atcgactcca
    15901 gcgcttcgct ccctagaagg aacaacgtat cttgcgtcac atcttcagtc tgctgaacct
    15961 gtggctttgt cacctgcgcc tgattcgcat actgcaacgc ttcctgaatc tgacggatac
    16021 gcagatcttt ctgctcctgc gcgaccactt cctgggttct taaagagtcc tgcaagtttt
    16081 tccgtcccag agcaatgttg tccttgagat ccttttctag ctcttgattc actttatcat
    16141 caacttgctg aatgtattgg gccaacttca tttgtgcgcc ctctgcagtt tgcccaacat
    16201 aagaaacagt caatggtaat tgctggttct taacagaagg ttcgatggta agtttttctg
    16261 gctcttcctg attatccagc gtttctgcta atgcagagaa ggcagaactg aagcgaccaa
    16321 ttaacgtctc ctgcaaatcc gatactttcg gtgcagcctg accatagata acattcatgg
    16381 cattgttata gccagcaatt tgccccacgt cgggctgagt gataattgct gttgacgtcc
    16441 atttctcctt cgctactgcc aaataaccaa tagccagggc aatagccaca atgacggaaa
    16501 tgataattgt catcttgcca cgccacaact gcactagtaa atcaatcaaa tcaatctgtt
    16561 ccgggtcatg gttttgccca gaaacattat tattttctac tctcatcatt accctaactg
    16621 acggaaattt ccaagtccgc atagtttagc gataatcccc gtgaatgcta taggataagt
    16681 gataaaaata atgaattatt gcggaggtaa caaaagacaa tcagggcgta aatagccctg
    16741 ataacaagat gttagtcgct gccaaagaga tcgcgggtgt agaccttatc tgccacatcc
    16801 ttaagctctt ctgccatacg gttggagata atgacgtcgg cttgttgttt gaaggtggcg
    16861 agatcacgtt ccaggcgaga gttgaagaat gagtcttctt tcatcaccgg ctcgtagatg
    16921 atcacttcaa cgcctttcgc cttgatacgc ttcataatcc cctgaataga agacgcacgg
    16981 aagttatctg acccgctctt cataatcaga cgataaatac ccaccacttg cggtttgcgt
    17041 gacaaaatgg catcggcaat aaaatcttta cgcgtgcggt ttgcatcgac aattgccgag
    17101 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
    17161 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
    17221 caaacgcctt cgataatctg acgagtattc agacctaaac tttctgcata actatccagt
    17281 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
    17341 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
    17401 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
    17461 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
    17521 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
    17581 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
    17641 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
    17701 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    17761 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    17821 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    17881 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    17941 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    18001 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    18061 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    18121 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    18181 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    18241 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    18301 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    18361 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    18421 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    18481 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca gttgtattct
    18541 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    18601 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    18661 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    18721 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    18781 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    18841 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    18901 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    18961 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    19021 accattttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    19081 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    19141 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    19201 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    19261 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    19321 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    19381 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    19441 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    19501 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    19561 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    19621 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    19681 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    19741 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    19801 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    19861 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    19921 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    19981 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    20041 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaaata
    20101 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    20161 aaacttgcaa ttgagatcgc aaccagcaat actgacaatg ggtaaatggg tgataaatac
    20221 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    20281 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    20341 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    20401 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    20461 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    20521 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    20581 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    20641 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    20701 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    20761 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    20821 aatgccaaga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    20881 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    20941 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    21001 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    21061 ccaatcaccg ggagctcgca gggacgataa caatgtaacc gcaaaaatta aaacaagaaa
    21121 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    21181 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    21241 aaattgcccg accatgccca gaaagtaaat tatcaaaata cttaaagaca tccctatgca
    21301 taggcaataa aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga
    21361 tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac
    21421 atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata
    21481 gttttttttc agaaagataa aataatgcct tcatttcacg tctagttgtt gtatttgaat
    21541 gcaaaataat gccacataca aatgccatta ttgacgataa catgatggag gcagttaaaa
    21601 aggcagtcgg aaatcttctc accaaacctg tatcaatata atcaacaatg atgggcaatc
    21661 ctaaaattat agacaaagca gcaagtaaaa cgccgataat actaaaaaag tatagtggcc
    21721 gttcaagcga gtaaagttta attattgttt tcagaatctt gaacccatca ctccaagttg
    21781 atagtttgct cacactccct tcagatcgct cgccatattt tgtattaacc tcaccatact
    21841 tcatacgtaa ttctaaagca tgaattgtaa gctcagtttc gatttcaaat ccccttgata
    21901 gacatggaaa agatttaaca tagcgtctgg aaaatacacg ataccccgac aacatatcag
    21961 taaaattccc tttaaagata gtctgaacag taccagtcaa cagtttattt cctcagcgat
    22021 gcccttttct gtatgtattt tgatctccat tctcttgacg acatcctaca accatatcca
    22081 attgattttt tatgagatgg ttaatcattt ctggagcaga acttgcatca taagtgtcat
    22141 ctccatcaac cattagataa atatccgcat ctacatctga gaacatacgt cttacgacat
    22201 tccccttgcc tttcagagga acagagtgaa ctgttgctcc agcatcctct gccacttgtg
    22261 ctgtactgtc agtcgaatta ttatcaaata catatataga agcagttgga atattcgttt
    22321 tgaaatcatt tatgactttt gctattgcct tctgctcatt ataacaagga ataattacag
    22381 cgatattaaa agagtgatac ataagcccct catataaact atttagctaa tgtgcaagta
    22441 attgtgtttt taacaaatcg tttactaacg aaaaatgaaa tattttgatg cgcaatatcc
    22501 taaaaccaaa ctaaaaagag aaaaggtgaa caatgtaaat attggagata aagctaagag
    22561 atcaaataaa acgccgaata aataactcat cgcccccata aaaaatataa atataaaata
    22621 tctagccttg gttgcagata ctttaaatga gcatcttgca tttgcaaaaa aagaaaaggt
    22681 tgctgcacat ataaaaccaa caatgttact tcggccttga ccaaaagcta aattattata
    22741 acacgcataa aatacccccc aatgtaaagc agtattgaca attccaatta aaaaataact
    22801 aaaaaaagaa ctggtcaata acttccctat ttttaacatc ctttattttg ctccagaagt
    22861 gaggttatta ctaatttgga tattttctat agaaaatacc cataaaacta caacgcctat
    22921 aataaaaatt cccatgggtg taaaaagaaa tggattagtt atagaaacgg taatactgaa
    22981 aaaaagtatt aacgacatca ttaatgatgt ttttgtactg tttaaaaaat agcctttcaa
    23041 aaagataagc aacataggaa ctaatgatat aaatacacca ataacgcctg ttttctgaag
    23101 tacctcaaga ggtactattt caatccttaa tcgatctaat ataaatgaac caaatcctct
    23161 tccaaacaag aacgtcgcta aatcaacatt tttataataa aaatataaat cattaaatct
    23221 aacagagtca gaatcttcac tgcgtgatcc gaccatgtaa agcataatta taaatagaac
    23281 gctgcccaat attattataa tagtatttaa atatagctta tgtaatttaa aactggctaa
    23341 taacagtgat aatgttgtaa aaagtaatac acctcttgat tctgtcaata atatcgcaag
    23401 cactattatg agacatttta aatatttttt gtcgacaaaa ctgataatcg caccgacacc
    23461 aaaaaagtag aaggatttgg aaaaaaaagc cccatcggga cgaaaaaaga actcgctagt
    23521 caatgataag tgttcataaa ttaaattaaa atttaacaaa ccgaaattca acaaaattat
    23581 gtatgaaata tataacagtg atccatataa tgcaaatgca ttaactattt taataaaaat
    23641 ctcacataaa aatactttta aagtatatct tagtgtaaat agatatataa gtgacagcat
    23701 aaagaggtat ggttgcacat caaacagtat atcatttatt ttattaccag ctaataagcc
    23761 gacaaagaag ccataaaaag gaaataaaag caaccatata aaaagactga cacatttttg
    23821 ggtgattgaa aaattgaatg taactaaatt aaaaattaaa agtataaaac taaatacaaa
    23881 taataaatat cgcaaaggga atattcccaa tggctccagt aatcttccac caccaccaat
    23941 aatcagttca atacataaaa atgttataaa aattttattt atattattca tattcgtaag
    24001 gtgatgtttt ttaaaatatg tttaaaatca acattaataa aatataaatt ttatcagcct
    24061 ttttccgcct taacttccaa aaacttaaag ccaagatcaa attgcctata taacctttac
    24121 ccaattgatt attcacaaag ctatcatcag ctaaggcatt agctatttta gttacttctt
    24181 ttcgatacca cttgtttctc accaaaccaa gccgtttgta ttttgcttta aaactaatgt
    24241 ttgctcccac ttggttgttt tcatgctgtc tgtacagcat tgttggtttt ctatcaatat
    24301 accacgaata atttctcgtc cgcgcaaatg aatataaaaa ccaatcatgc agacagatat
    24361 cttgtgcagc atttttatta tttatgatga acttcttaaa ttcaataagt gtttcttttt
    24421 ttataacata tgtacacccc ggacctgctg cttcaaaaaa ataatcgaat tgagtttgcg
    24481 actgagcctt atcaactaaa tttttttgcc ctgatggata atatgcaatt acattgctgg
    24541 aataacaatt cgcattatta aataccaagc agtcaattgc attttttatt ttatctttat
    24601 accaaatatc gtcctgatca ctaaaacaga tataatcata gttttcaaga tcgacatctt
    24661 taattaatct ataaaaattt ttacctgccc ccccaaactt attgacgctt ggaagtatat
    24721 ttataattga gggatatgat aattgaaatt cttcacaaat atttaatgta ttatcagttg
    24781 ataaatcatc tgatataaaa attgagatgt ctacatcttt ttgttcaata atacttttta
    24841 attgttcctc aatccagcat tcgccattat acgtagccat aagtacagct actctttttt
    24901 gatcagaact atgcattata acgaccgtcc ccagtaattc ctcttattcc atccagcgta
    24961 gccaaaatat gttttttcat atattcaatt ttattttttt gagttgcgat aattaaagtc
    25021 gtatgaatta aagaaaaaac aacttcccta atagagagta aaagaggcac atgatttttt
    25081 ctaagcaaaa gaaatgcatt tcttactcga taataacgac gtactggcga gtgaattggt
    25141 atgttaatcc cacagatttt tactgactta tccccaatag catgaaccat ttctatatca
    25201 ggaatgatat gaactaatat gccatagttt aagcatctaa gacaccattc tgtatcgaca
    25261 taatcaataa aaagttcctc atccatcatt ccaacaatct caaaaacttc ttttgaaacc
    25321 atggttccgg atgagattgt tactgaactt ttaaacggtg tctgtccttc agtgacatga
    25381 attttctctc gaagaccatt ttttttgata ttacaaatag gataataatg tgacttatct
    25441 ctatcaataa aaactgggcc agctattttt gcattttcat tatccatata gcaggaaagt
    25501 tttttgataa actcattgcg aatacggcta tcttgatcaa aaaaaattat cttctctgcc
    25561 cctttttcaa gagaatatcg aatcccaata ttttgtgctt ttgctatacc ttcattccta
    25621 ccaagacaaa ttaaatgaac tttttcaatg tctgctaatt tatctttcaa ggtgaaatta
    25681 gtactattat cgactactac aacgtaacaa ttttgctcct ttagttcagt gatcagtgca
    25741 ttcagatttt taagttcggg attatatgtc acaatgacag cgtaaatatt actattcatt
    25801 atccaagtga ctcagtaatt ggttaatttt agttttcata tacagaacac attctagtat
    25861 aaagccattg taataacaga gcatctaaaa ttacacgtat gctccaggat attgcggcac
    25921 ccagtaaccc atatttttcg accataaaat acaaaagggc taaataagga accaattcag
    25981 cacaatgaat caaagcagta attttagatt ttcctaatgc ctggattgca gagaatggta
    26041 tcattgccag cgcattgaag aaaaaaccga tcaataacac atttaatata ttaaaggaat
    26101 taatttggta attctcacca aaccatattt tcaatacaag ccctgaatat aacaagccta
    26161 tgattatcac cggcaaacag attagaaaca ttaaaagcaa tgatttattt acattacgtt
    26221 taaattcttt aaagtctttg atgttactta accttggaaa cactgccctc ccgattgctg
    26281 caggtattat tcctaatttt agaataacct ccgctggtgc agaataaaat gcaactttat
    26341 ctgcccccat aatatttgac actataaatc gatcaaaata caccatgacc ggacttatga
    26401 tattacttac tgtcatccaa ccgccaaaga aaaagagtcg tttaaaggtt ttataacaaa
    26461 aatgaacccc cgaaatttta atatcattcc tgacataata ggcagagact aaaatcgaaa
    26521 taactcttgc aaatattaaa ccagcaaccg ctgccgacaa tgtagcacta taaaaaacaa
    26581 atatggccgg aattcccgcg atgcaagagc ttgatataga tttttgaata tttacaatgc
    26641 caaatttttc atccccctca agaatggctg accataattg atttagaata aataatggaa
    26701 tgcaaatagc caacagttta aatgctagtt gtatatcact atgttcaaca ccagaaattt
    26761 ttagataatt aacaattcca tcagagaaaa tcaataataa aaaagcacca aagcatgaaa
    26821 atagcaccaa aaaagatgta cttgttgaaa ttaccttggt tctttcatga tgattatcac
    26881 gatgaattgc aatttctcta ataacagagc gagtcaggcc tacatcaaaa attccagcat
    26941 acccaacaag tgcaattgca attgtataaa caccgaaacc ttcaggcccc aaacctcgag
    27001 cgagaaatcc tagcgagggg atagcaatta aagttggtat tgcatagcca aaaaggttcc
    27061 agacactatt ttttattatg ctcattatta catttcttta tcatataaaa taatcagcat
    27121 ctacaaattt ttgaccatta atatctttat cagaaagcaa tggattttga acctgccatt
    27181 ctatatttat acttttgtcg ctccataaaa tcccacgctc ttgcattttt gaatagaaat
    27241 tatttgtttt gtagacaaat tctgcttcat cactaagaac gcaaaaccca tgagcgaatc
    27301 cttccggtat ccataattga cgcttattct ccgccgacaa atttacacca acccacttgc
    27361 caaatgttgg tgatgatttc cgaatatcaa cagctacgtc aaaaacttca ccgacaacac
    27421 agcgtaccag ttttccttga gcataaggtt ccagctgata gtgcagtccg cgtaacacac
    27481 ccttagttga tttagaatgg ttatcctgaa caaattcaac ctttcgtcct acagcctctt
    27541 cgaaaacttt ctgattgaag ctttccataa aaaagccccg ctcatcacca aatacttttg
    27601 gttcaaaaat tagcacatca ggaatttcag ttttaattac gttcatttta ttaataacct
    27661 ttaatcattt tcagcagata ctgaccataa gcatttttct tcagcggctc ggctaatacc
    27721 ttcacttgcc cagcatcaat aaagccttta cggtaagcaa tttcttccgg gcaggaaact
    27781 ttcagcccct gacgctcttc aattgttgca atgaagttgc ttgcttcaat aagactttga
    27841 tgtgtccccg tgtccagcca tgcataacca cgccccatca tagcgacaga caaacgtcct
    27901 tggtccatat aaatacggtt aatatcggta atttccagtt cgccacgtgc agatggttta
    27961 aggtttttcg ccatttctac aacgtcattg tcatagaaat aaagcccagt aaccgcatag
    28021 ttactttttg gttccagcgg tttttcttcc agactaattg ccgtaccgtt atcatcaaat
    28081 tccaccacac cgtagcgctc tggatcattt acgtgatagg caaaaaccgt tgcgccactc
    28141 tctctgttaa cagcggtatc cattaatttc ggcaggtcgt gaccatagaa gatattatca
    28201 ccaagtacca gcgcacaatc atcaccacct ataaattctt caccaatgat aaatgcttgc
    28261 gccagaccat ccggactcgg ttgtacttta tattgcagat taagccccca ctggcttcca
    28321 tcaccaagca attgttgaaa acgtggtgta tcctgcggcg tactgataat cagaatatct
    28381 cgaatacccg ccaacataag cgttgaaagc ggataataaa tcatcggttt atcataaatc
    28441 ggtagcagtt gtttgctcac agccatagtc acaggataaa gacgagtgcc ggaaccacca
    28501 gccagaataa tacctttacg cgttttcatt tcaccattcc ttttaattca tctcgctctg
    28561 gcatcatgag cgagatgcaa taatttgtta aattgccgtg gtcgtaaata attcgttgag
    28621 catacgtttc acgcccacct gccagtcagg caagacaagc gcaaagttct gctgaaattt
    28681 ttcggtattg aggcgagaat tatggggacg acgtgctggt gtgggatagg ccgttgtcgg
    28741 cacggcatta agtttgttaa gagcaaggtt gatccccgct ctgcgcgctt cttcaaatac
    28801 cagagcggca taatcgtgcc aggttgttgt accaccagca accagatggt acaaaccagc
    28861 aacttctggt ttatttgcgg ccacgcgaat ggcatgagcg gtgcaatcag ccagtaattc
    28921 agcccccgtt ggtgcgccga attgatcgtt tattacagcc aactcttcgc gttcttttgc
    28981 caggcgcaac atcgttttgg caaagttatt tcctttacca gcgtataccc agctggtacg
    29041 gaaaataaga tgctttgcgc aatgttcttg taacgctttt tccccagcta gcttggtttc
    29101 accgtagaca tttagcggtg ctgttgcatc cgtttccagc catggcgtgt caccgttgcc
    29161 tgggaaaaca taatcagtgg agtagtgtat aacccaggct ccgacttcat tagctgcttt
    29221 tgcaatcgct tcgacgcatg tcgcgttaag taattgcgcg aaattcggtt ctgattccgc
    29281 tttgtctact gcagtgtgag cagcagcatt aacaataaca tcggggcgaa tttttttgac
    29341 ggtttcagcc acaccttcgg ggttgctgaa atctccacaa taatcagtgg agtgaacatc
    29401 gagcgcaatc aagttaccca gaggagccag agcacgctgc agttcccaac ccacctgccc
    29461 tgttttgccg aaaagcagga tattcattac tgacggccct catagttttg ttcaatccac
    29521 gactgataag caccgctttt cacattctca acccaatttg tattagccag ataccattcc
    29581 accgttttac gaatcccgct ctcaaacgtt tcttgcggtt tccagcccaa ttcgcggcta
    29641 attttatcgg catcaatggc gtaacggcgg tcatgccccg ggcgatcggc aacataagta
    29701 atttgctcac gataagattt ctctttcggt acaatctcat ccaacaaatc acaaatagtg
    29761 agcactacgt cgatgttttt cttttcgtta tgtccaccaa tgttataagt ttcacccgct
    29821 ttaccttcgg ttacgacggt atataacgca cgagcatgat cctctacata caaccagtcg
    29881 cgaatttgat cccctttacc ataaataggt aatgccttac cttccagagc attcagaata
    29941 accaatggaa taagtttttc cgggaaatga taaggaccat aattgttcga gcaattagtc
    30001 acaatggttg gtaaaccata agtccgcttc caggcacgta ccaaatgatc gctggaagct
    30061 ttagacgcag aatacgggct acttggcgcg taagctgtcg tttccgtaaa tagcggtaac
    30121 gcttcgttat tatttgcttc atccggatgg ggtaagtcac cataaacttc atcagtagaa
    30181 atatgatgaa agcggaaact ttttttcttt tcatcattca gagcagacca gtaattgcgc
    30241 gccgcttcta aaagaacata agtccccaca atattggttt caataaatgc cgcagggccc
    30301 gttattgaac ggtcaacgtg gctctctgcc gccaggtgca tcaccgcgtc tggctggtgc
    30361 tgtgcgaaaa tacgcgacat ggctacagca tcgcagatat ctgcatgctc aaaagcataa
    30421 cgttcagaat cagaaacatc agcgagtgat tccaggttcc cggcgtatgt taatttatcg
    30481 acattaacaa cactatcttg cgtattattt attatgtgac gaacaacagc agaaccaata
    30541 aaccctgcgc caccagtaac aagtatcttc acttttttat tccatatagc tatagagcat
    30601 gctgtgaaat agactgctcc agatttgatt aatagaggta ttaatacacg ctaccgcccc
    30661 tggcttaaca gctaccagtg cactgcatgc atctcaacga tgtggcgagc gttagccact
    30721 cgcgccaaat ccgaaaaatt caaacgttaa ttgtcttacc aaacagccgt agaaacaagg
    30781 aaaatcctgg aaaaatttga ataatatgag ctatgtaact tcttgttttt ataggcttta
    30841 taaaaacggc aacggcagga aaattcaatg agcaaaactt atgccacgaa tcttcactgc
    30901 cgttataatt ttcttatcaa ccgttacatc cggtcagatt ttcattattc gcttaacagc
    30961 ttctcaatac ctttacggaa cttcgcccct tctttcaggt tgcgtaggcc atacttcaca
    31021 aacgcctgca tatagcccat ttttttgccg cagtcgtaac tgtcgccggt catcagcatt
    31081 gcatcaacgg attgtttttt cgccagctcg gcaatagcat cagtcagctg aatacgtccc
    31141 catgcaccag gctgagtacg ttccagttcc ggccaaatat cggcagaaag cacatagcga
    31201 cctacggcca tgatgtctga gtccagcgtc tgcggctgat ccggtttttc gataaattca
    31261 acaatgcggc tgactttacc ctcacggtcc agcggctctt tagtctggat gacggagtat
    31321 tcagagaggt cacccggcat acgttttgcc agcacctggc tgcggcccgt ttcgttgaaa
    31381 cgtgcaatca tggcagcaag gttgtaacgt agcgggtcgg cgctggcatc gtcgatcaca
    31441 acgtctggca gtaccacgac aaatgggttg tcaccaatgg caggtcgcgc acacaaaatg
    31501 gagtggccta aacctaaagg ttcgccctga cgcacgttca taatggtcac gcccggcgga
    31561 cagatggact gtacttccgc cagcagttga cgcttcacgc gctgctcaag gagtgattct
    31621 aactcataag aggtgtcgaa gtggttttcg accgcgttct tggacgcgtg agttaccagg
    31681 aggatttctt tgatccctgc agccacaatc tcgtcaacaa tgtactgaat cattggcttg
    31741 tcgacgattg gtagcatctc tttgggtatc gccttagtgg caggcaacat atgcatcccg
    31801 agacccgcta caggaataac tgcttttaaa ttcgtcatta tttcatccac ctgtaaaatg
    31861 gttgctgaat tatagcttgt tcgatttttt tcgccagcat caattaccct gaattgatta
    31921 ctgaattact tgtgatgtta cgccgcttcg ttgtggattg cagtagcatt gttcctaagt
    31981 atggctccat ttttccagga atggtcgcaa atctactccc tccgttccgg caatctaaag
    32041 ttaatctttt ccacattaac aatatggtga ttaatcctgt cgatatcgac ggagctttgc
    32101 cctctttcat tcaccgcatg aacatttgca agagacagca gtgtttcttt tttcgccata
    32161 aagacgccgc gaacgtcttt gcgcatgtcg aagttcatgc tcaatgctgg tccaactgag
    32221 gattcctgca tcacattgat atttcgcata aaaagatgtt gcggcttgtt gtgtaactcc
    32281 agcgaggcac gcttcatctc aatgttagtt agcgacacaa aggagacggc attcccggcg
    32341 gagatttgga tgccgcgcaa tttataagca agatgggtat tatccagttg aatattattc
    32401 actcggaaat tttgcggtat cgagagatat ttgcctttaa ttaccccata gccgattaac
    32461 atcccggcgc tattaatcat ttcaatatta tcaatcacga aattgtcaca accgtaaata
    32521 gcgactgtcg cgttatcaat gcccgctttc ttactgaaat ccggcgtgat attgcgggca
    32581 ttgatattac gaataacaaa atgtttacca ttttcaacat ggatcaactg ccgacaatcc
    32641 gatcccgtga tattcgccac gacaaagttt ttcactgcct ggtcttccgg gtagttgtta
    32701 tcataagtgc ttcccgcaag gcctatgccg atgccccagt tgattttgcc gttggtacag
    32761 ttgatacgct cgatgacatg gtcagatatc aaaatatcac tgtcgttaat tgccacgttc
    32821 cattcaatgg cgtcgccctg taagtcgctg aacttacagt tggtaatgtt ggcaccgata
    32881 atctggttat gaaatccctg gcgtaagatg gcgtaattag cgtggctgac agtgaggtta
    32941 tcgatggtca ggttgcgcat gacccgtttg tttttgccgc caatataaat ctgcattacc
    33001 gggccaaagc cgctcatcgc cagccctttg atggtgcagt cagaaccacg cacatccagg
    33061 gtaatgttat gcataccgcc gccctctccc cctgtcacct agctgccgtc ctgtaagaca
    33121 aatcgccctc tgccgttgcc gcgcaggctt ccaaggatgt gtaacgtttt gccgggaggg
    33181 atgaagatgc cggtgttgat attgtcacaa acgaatccgg caggcacgac gaccgtttgc
    33241 ccttcgctga aggcttgttt aaatgaggcg atccagtcat gtgggttgta gtcgttaatg
    33301 ttaacgcttt gtcgggcggg aagtgcgcga gcgaaagggg tatggaggaa ggcaagcgcc
    33361 gagcttgccg tcaggaaggt gcgtcgggag agtgttttaa atggcatacg ttctcctcta
    33421 taaagcctgc agcaagctgg cgagttctcg attaatcacc tgctggttaa aatcgtgttc
    33481 gactttttcg cgcgcacgtt tgacgaccgg agccagttcg tcggtatcca gttggctaaa
    33541 cgccgccaga cgttgtgcca gtgcgcgagc atcgttctca ggcaccagcc agccggattt
    33601 atcggcctcc accagttcag gaattccgct atgcagagta gaaaccaccg gaataccgac
    33661 cgccatcgct tccattagcg ccaccggaat gccttccata tcgccatccg cgcccgttac
    33721 cgatggcaac aggaagacat ccgcgtcgtc gagcatctcc ttcacttcat ggctcggttt
    33781 aaagcccggc atctctacca catcttccag ttgatattgt tcgatgaggg tgcgcaggcg
    33841 tcgttcccac aggccaatgc cgaggatgcg atagcgaaat gccacgccct gctctttcaa
    33901 ctgacggcag gcttcaatcg ccacatgcag gccttttttc tcggttaagc gtgcgacaga
    33961 tatgatttcc agcggcgttg cgggcgcttt caccggacgc gggctaaagc gcgtcatgtc
    34021 cacgcccata cgcgatacgg cgattttttc tctggggcag cccatttttt gcagccttcc
    34081 ggcccacaga tcgcttatcg gtaacatcag gtcgccacgg cgaaacagtt gttgatattc
    34141 gggagtgtag tggttgagca cttcccgact ggagatatca ataccgtgga agatggtggc
    34201 aattttgccg cgaatgatac ccagttcgcg tagttttgct gcggttaccc ccgccgggcc
    34261 aaaatgagcg atgaacacat cggcgcgaaa cggtgttgcg acctgaccac aaatggcaga
    34321 caaaatcagg ttccgcgact cggcaccata gcgtttgagg ttgagcgcat gccaggtatt
    34381 tttacgatga atgccacgta aggtctggct ggcgcggtgg cgcagtttcg ctactttgcc
    34441 cgcaggttcg tcctgtaacc agcgggttct ggcgacaagg ttgtatttcg tccatgccgc
    34501 gtgggtgttc tctgtatcgc ctttttgcaa agcgacaatc tccacttcaa agcccatatc
    34561 aataaacgcg gtaatttgat tgaggacgaa ggtttccgac gacagcggga atttcaataa
    34621 gaagaagccg accttcattt cacctcctcg atgcgctcaa gcacagactg caccatctgc
    34681 attcccgtct gacgttcgcg actgacggct tcgttaagtc gagcgttcag caccggaagc
    34741 tggcctaagg tatccgcaac catcgcttgc aggctgccgt ccagtaaatg acggatatca
    34801 atcgccatct ctggcagtcc cagctgctgc ataatcccgg cggatttatg ttcatagttg
    34861 atggcaatcg ccggagtagc aaagttcatt gagataatgg cggagtgcag gcgcgtaccg
    34921 acggtgagtt cacaggctcc gagaattttg cccatttcca gatcgttgag ttcatccatc
    34981 actacgtggt aacgggcagg atcgctgatg tgctggcgca gattgagcgc caccatgcgg
    35041 tcgtctttgt tatagctgtc gatacccgta caggtggaga gcgcaatcac ctgatagcct
    35101 tcatcgagaa tgcgattgac caccccggca aaggcttttt catacgcttg ttgagtggtg
    35161 ccgagacgtt tgtcgaacgg tgccagttcg cgcagggtaa ttgccactgt tttctgttgt
    35221 gcggcaacgt ccagccagtg ttgaacggca tagctggcgg tgaagtcttc ggtgtggtga
    35281 tcgaccagcc acgcggtatc gacgccatgt tccacttttg cggtggtgat attgctgcgt
    35341 ttcatcaaat caaggctgac cgattcgcgc aggatcagcg cgtcgcagtg accaaagaca
    35401 tagttcgcca gttggttaaa ttgctcatcc tggaaggggc cgacgctgtg accaatcata
    35461 aacagcggct ttttcgccat aaatgtgcaa agcgcatgtt caaactgcgg cacgccgtag
    35521 agatcgacaa aaaacgatcc gccgacctga ataatggcgt cgtaacctga cagcaggcgc
    35581 acgaagtcgg tgaatccctg ggcgatggcg atattgcgca gcttgccagt gtcggtgacg
    35641 cgtgagagca atacctgatg ctggtagcgg cgacggagga cttttttaac gcgcccgaca
    35701 acgcccgccg cgctattgtg ttgtgtcatc tgcaggaaca gcggatcgcc cattaccggg
    35761 cggttgagca gccaggaaga actgaccgga tagcggctca tcacgtccac ttcggtatgt
    35821 ggattgagga tgttgatggc atcaagtaag ccgcgcaaga tggcgctgtc gccacgattg
    35881 ccgcaagtgt ggttgcccag aataagtaat ttcatttttt cctcataaat ttgatgccag
    35941 gtgaggctgt gtttgttgtg ttcgttgccg ttttttgcct gatgcgacgc tggcgcgtct
    36001 tatcaggcct acaggtcccg agcacagaac cgtaggacgg ataaggcgtt ttacgccgca
    36061 tccggcaacc gttgtcggaa ccgaaaacag caattcaccc cgcccgtaaa agcattttca
    36121 ttttttcgct gcgacaaaac tgacgcttca cttccaccac cagcggatgg cgtgacagca
    36181 caatcatcac cacaaacgcc agcacgcccg cggctatttg caccgccagc agcatcccca
    36241 gcgccagttg ccctttcagc acaatgccca gcgcataact gaccaccagc gtcggcaacg
    36301 agagataaaa cggcagccac aaactcagga tgtactggcg ataactggaa ccaagaaccg
    36361 gtttaatcat cacgaagtaa ctcagaatgg tgttgataat ctgcaccagc aggaaaccga
    36421 gcgtgacgcc gattgcgccc gccatctgcc caccaataac aatcgccgga ataaacagaa
    36481 atgttttgaa tacgttgaat ttaaagctga tatcgacccg cgctttcgcc atcagcagcg
    36541 aaccaatcgg gttacccacg gagcgcagca gacccacaat acacagcaat tgcagcaccg
    36601 gaataatgct gttccacttt tcgccgaata ccagcggtac gaagttattc gacaccacca
    36661 tcagccccag cagcgccgga aagttgataa tccccactat cgacagcagc ttgtagaagt
    36721 taacacgcag cttttcggtg tcgtcctgaa ttttggcaaa cgccggaaac aacacgcggg
    36781 tgatgattgg gttcagcttc atcggcggta cgacggccac gttgtacgcc aggttgtatc
    36841 cccctgccac gcccgcgcca agaatacgcg ccagcaccag cgttgaaagg ttggtgttga
    36901 gatagtggat gatgctgtcc gccgtcagcc aggcaccaaa acgtaagttc ggtgccacag
    36961 acgccagcga gaagtgcaga ccggggcaat aaattttgcg tccaaagtag ccaaacagca
    37021 gtgttctcac cgcactattg accagataac cgaggatcgc ggtcatcgcc agcggccaga
    37081 aatgggcgct aactaccgta aaagtgaagc ccgccagcac cgcgctggtt tcgatcatgc
    37141 cgattttgtt gaactccagc tccttctgca tcaacgcgcg gaactgttgc ccgtggggga
    37201 tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc caggtccggg ttattcagca
    37261 cgtcgccgat gacatcactc aacaaaaaca ccgccacaca taccacaagc cccagcccga
    37321 cgttcagcca gtacagagtg gtgagttcaa ggtgactgat ttcttttcgc tgaataatcg
    37381 agttagcgat accgaagtca gaaagcgtat ctgccagcgc gataatcacc agcgacacag
    37441 tcagcaggcc gaactggtgg ttgtcgataa tccgcgccag cacggtcatc tgcaccagcc
    37501 cgaggccgat gatgatcacc gtggcaatag ccgaccactt cgcgccgctg atggtttttt
    37561 cacgtaagct catatcaata tgccgctttg ttaacgaagc ccttgaatac cgtcaggaaa
    37621 acgattttga tatcgaacca gacgctccat tcgcggatgt actcaaggtc gaactcgacg
    37681 cgtttttcca ttttctccag cgtgtcggtt tcgccgcgcc agccattaat ctgcgcccag
    37741 ccggtaatgc ccggtttcac tttatggcgc agcatgtagc cttcaatgag ctggcgatac
    37801 tgttcgttat gcgctactgc gtgcggacgt ggaccgacaa tcgacatccc cccggtcagc
    37861 acattgataa actgcggcaa ttcgtccagc gaggtgcggc gcagaaagtt ccccacttta
    37921 gtgacgcgcg gatcgttctg cgtcgcctgg gtcaccactt tgtcgttctc catcactttc
    37981 atggaacgga acttccacac tttgatcggc ttgccatcca taccatagcg agtctggcgg
    38041 aaaataaccg gccctggtga actgagtttc accgccagcg caatacagca cagcaccggg
    38101 gagatcagca gcagaataag cgtcgccagc acaatgtctt ccgcacgttt tagcaggcgg
    38161 ttaacccccg aaagcggcgt gtcatacagc ggcaccaccg gaacgccgtt catctcttcg
    38221 agacgtgaat ggagaatgtt gaaggtaaag acgtcgggga tcagcagcac cgaacaggtg
    38281 gtatccgcca gttgatggac cagttttttc actcgtgcgc cgtcgcacat ttgcatcgcg
    38341 atatagacgt tatgaatctt gcccgctttt gcatcctcga ccagctgttg caggttaccc
    38401 gcccagtcgt tagaaacgcc gcccggtttc gggtcgtggt aaacgcccac cacttcaaac
    38461 cctaaccacg gctgattacg gaaactctcc atcagcattt gtccggcggc taaatccccc
    38521 gctacggcga ccatccgctt gttgtagcca tgattacgca gccagcccgc cccaatgcga
    38581 atacatgaac ggcaaaccac cagtccgatg ctggtcagcc cataccacgc cagccagatt
    38641 ttcagttgcg tgtcgaaatc attgttgaac gccaccagtc cggcgctgaa aatcacgctt
    38701 aaggtccagt tttgtagcaa cagggcaaat tctgtcgctg cccgaacacc gcgccatgag
    38761 cgataaaaat cggtgatgcc gcccagcatc tggaacacca ccagcgtaat cagcgccacc
    38821 aacaggtgca tgtagaggaa tgacagtccg ctgacttcgc aaaccagcca tagcccggca
    38881 aacatgatgg tgatatctga aaagcgttgc accatagaga ttaacgatgc attggttttc
    38941 gctcgctcac gcttttttag atttatcatc gttgttcctg ttattagccc cttacccgca
    39001 gtggggtaag ggaagatccg acattactcg ttcagcaacg tcagcagagt tcgcgttcgc
    39061 gcttccatca gcggcacatc accgcgcgat tccacattca ggcgcaccac cggttcggta
    39121 ttggaggtgc gcaggttaaa gcgccagtcg gcaaaggtca tgctgatgcc atcgctgcga
    39181 tccaccgcca gcgcctcacg gctaaaatgc tgttccacgc ggttaatcgc ctcaacgggt
    39241 tgcgccagtt tgctgttgat ctcaccgctt gccggaaacg ccgccatccg gtcgcgcacc
    39301 agttcgccca gcgtttttcc tttcaggcac accagttcgg cgaccagcag ccacgggatc
    39361 atgccgctgt cgcagtaagc gaaatcacgg aagtaatggt gggcgctcat ttcgccaccg
    39421 tagatggcgt cttccttgcg catccgttct ttaataaagg cgtgtccggt tttcgacatt
    39481 accggcgtgc cgcctgcagc agtcaccaca tcaatagtgt tccaggagag acgcggatca
    39541 tggatgatct tcgcgccggg atttttttcg aggaacgctt ctgccagcag gccgacaatg
    39601 tagtagccct cgataaactg ccctttttcg tcaaacagaa aacagcggtc aaaatcgcca
    39661 tcaaaggcaa tgcccatatc cgcgccgtgt ttgatgaccg cattgcgggt gtcgtcgcga
    39721 cattccggca gcagcgggtt aggaataccg ttggggaaat tgccgtccgg tgtgttgtgt
    39781 actttgatta attccacggg cgcgccgagg gctttaaagc gggcttcaat ggcgtccacc
    39841 accggacccg ctgcgccatt cccggagttg atcaccagct tgagcggcgt gagatttttg
    39901 acattgatat aaccgaacag gtgatcaacg taagcgtcac gcaggttgat ttgctgatag
    39961 cgaccgcgtt tggtttcatc gacgggagga aagtcgttgg cttcagccag acgctggacg
    40021 tcgcgcagtc cggtatcacc gctgatcggg cgagccccct cgcgcaccag cttcatgccg
    40081 ttgtaatcca tcggattatg gctggcagta acttcaatgc cgccatccac gccgagatgg
    40141 aacgtggcga aatagatctc ttcggtgccg gacataccga tatccagcac atcgacgccc
    40201 gcatcctgta aaccttttgc cagcgccagt tttaaggttt cgctggtgag acggacatca
    40261 ccgcctaaca caatggtttt cggtttgaga aattcgccat aggcgcgacc aatgcgccag
    40321 gcgatatctt cattcagttc ttcgcctaat ttcccgcgaa tatcataggc tttaaagcag
    40381 gttaattttt tcatgtcgtt accctttttc aggcaatagt tggccctgac cgaaacggcc
    40441 aattttattt ttgtcgttat ttacaccgcg gtttcgcatt cattgcctga tgcgacgctg
    40501 ccgcgtctta tcaggcctac aaaaccgaac cgtaggtcgg ataaggcgtt cacgccgcat
    40561 ccgacaaaca gcgcctgatg cgacgtttac acccgtccgt agcgatccgc gaagcgcacc
    40621 acatcatcct cttcgagata agagccggag cgcacttcaa ttaaatcgag cggaattttc
    40681 cccgggtttt ccaggcagtg cgtcgccccc agcggaatat aaatggactc gttttcacca
    40741 agcagtttga tatcaccatc aatggtgact tttgccgttc ccgcgaccac tacccagtgt
    40801 tccgcgcggt gatggtgcat ctgtaccgac aaaccctcgc ccggtttcac ggtgatgcgt
    40861 ttcacctggt agcggtcgcc cgcgtcgata gagtcatatt tgccccacgg acgatacact
    40921 tcgcgatgca cccgatgctc atggcgacca tcggctttga tctgctcgac cacttttttc
    40981 acatcctgta ccgcgttacg gtcggcaatc agcaccgcat ctttggtctg caccactacc
    41041 agatctttca ccccgacggt ggtgaccagg cctgattcgg catacacata gctgttttca
    41101 gttttgtgat taatcacatc gccgtggcaa acgttgccct cggcggtgtg ggcgctgatc
    41161 tcccataatg aagaccagga accaacatcg ctccagcccg catccatcgg caccacaaca
    41221 gcatctgccg tacgttccat gaccgcgtaa tccaccgact cttccgggca ggcgagaaac
    41281 gcttcttcat ccacacgaat aaaatcgaga tccggatcga cggcgctcat cgctttttcg
    41341 caggcatcga ggatatccgg gcgatatttt ttcagttctt cgagatagcg tccggcgcgg
    41401 aacaggaaca tgccgctgtt ccagtaatat tcgccgcttg ctacataggc ctgggtggtt
    41461 tccagattcg gtttttcgac aaactgcgcc acttcaaagg ccaccgtatc ctgctcaccc
    41521 gcagacactt caccgcgacg aatatagcca taaccggttt ctggtagatc cggcacaatg
    41581 ccaaaggtca ccagcttgcc cgcttcggca taaggcatgg cattacgcac ggcggcacgg
    41641 aacgcgtctt catcggcaat cacatgatcc gccgccaata ccagcattaa cgggtcgctc
    41701 tccgggctat gacgttttgc cgccagcgcc gccagtgcaa tggcaggtgc agtgttacgc
    41761 cctgccggtt cgagaataat gttctcggtg agtttgttca gttgacgcag ctgttccgca
    41821 acaataaagc ggtgctgctc attgcaaatc accaccgggc tttcgcactc cacgccgttc
    41881 aggcggcaga tggtggtttg cagcatggtg agatcgcctt tcaggcataa aaactgcttg
    41941 gggtaaagta cgcgggaaag cggccataag cggctaccgg agccacctgc catcacaact
    42001 ggatagagtt tcgactgcgc cataattatc cccgaatatc atttataaat tgacgtaaca
    42061 cgttctcttt atcgagcgtg cgttcggcat attcacgtgc caccgtgtta tgtttgggca
    42121 gcaggagcgc ctgacgaatc cccgccacca gcgcctcgac cgattccggt tctacgcaaa
    42181 ccgcaatacc cggaaaggtt tcgcaaagct gccccagttc tgtgtgggcc tctgcggtaa
    42241 tcaccgcgtt accgcctacc gccagaatat tggtcagttt cgacggcaat acggcatctg
    42301 ccgcgccgcg tttttgcacc accagatggc aatcgcccat cttcagcagt gcgggtaaag
    42361 cgtcatacga ttgcagcggg aaaaattgca tgttgcgcag tccacgctgc tgcgccattt
    42421 tttccagccg cgctttgccg ccgccctgcc cgacaatggc aaaaatcagc ggttcatcgc
    42481 gcaggagatc ggcagcttca ataacgtttt ccagcccctg cttttcacca atattgccgg
    42541 agtaaagaat gatttttttg ttatccggca ggccaagctg gttacgaagg gcatcaacat
    42601 cggcgtctgc aacatgctga aaacgggcaa tttccgacca gttggggaag aagatgacgt
    42661 tatccgccgc cacgcctttt tcgatggctt tattcatcat cgaacgcgaa atcgtggaaa
    42721 cgttatcgac gttatgcagt ccgctacgtt cgaacgccgt tgccagctgt gccactttgc
    42781 cgcctttgcc ttttccggca aggcccagcc ccagcatggc gtccacttcg taatcctgaa
    42841 tatgcagcac ggtacgcgca ccagagagct tcgccagcag gcgcattccc ggcgtgcaaa
    42901 acagcgttgg cactacgcca ataatgcgat ccggcttcca gcgacgttgc gccatcagtg
    42961 gaaagaaact gctgacggca aaactgccga gatgcaacaa gcgtttcagg gtgctcggct
    43021 gttttggcac gtacagcggg cagcgccaca ccgtggcggc cccctcttct cgtttgtagc
    43081 gccaggcgga atagttctca cccacctgcc attgcgggta gtaaggcggt gcggtaatga
    43141 cccgcacctc atgaccttgt gccgccagcc attccaccat ctcgccggtg tatttgccga
    43201 tgccggttaa ctccggcgag tagttaatgc cgtagaccag tattttcata atccgggtac
    43261 tccggcacgc ttctcagcga gaaaataggc gcggctgtta gcgtgaacat cgttactggc
    43321 gagcagcgcg tccggcgtca gccagcggta atcgtcatgc tgctcatccg gcagtaacag
    43381 ctcttcttcc gctactctga agcgaaaacc gagcaccaca tagtgagtgg tgaaatccgt
    43441 gccggagaag ttatcgtcat aaaaatgctg ccagacaccg taaaactggc ctgctgttat
    43501 cggcagacgc agccccagtt ccgccatcgt cagccgctca aatgcggctt ccagcgtttc
    43561 gtctttctgc acgcgccctc ccggcacaaa ccagtaaccc tgcgccgggc ggttggttct
    43621 tttgccaagc agaaactcgc cgcgactgtt ctcgacaata aagtcgagag agacaagcgg
    43681 tgtggagcgc actacagtgg caaagtcttc ctgacgtaaa aacatcattc cccccgaaag
    43741 cggtcttgat tctcaaggaa ccactggtaa gtgctggcaa gccccgcttc cagtgagatt
    43801 tcgtgatacc agccaagctg atgcaggcgc gtcacatcca gcagtttgcg cggcgtacca
    43861 tccggtttgc tggcatcaaa aaccacccga cctttgtaac ccaccacttt ggcgatggtt
    43921 tgcgccagct cgcggatagt gcagtcaacg cccgtgccga cgttaatgtg cgacagcatc
    43981 ggttgggtat tctccagcca gacttcatgc gccagctcca tgacatgaat gctcgccgcc
    44041 gccatatcat cgacgtgcag gaattcacgc atcggtgtac cgctgcccca taccaccacg
    44101 tccggcgcat tctgtgccgt cgcctcgtgg aagcgacgca gcgatgctgg tatcacatgg
    44161 gagttactcg ggtggaagtt gtcatgcggc ccgtacaggt tggtcggcat gacagagcgg
    44221 taatcgcgtc catactggcg gttgtaagat tcgcacagtt tgatcccggc gattttggca
    44281 atggcgtaag gctcgttggt cggctccagc gtgccctgca ataactcgct ttctgccatc
    44341 ggctgttttg ccagtttcgg gtagatgcag gacgatccga gaaacatcag tttgttcacg
    44401 tcgttctgat gcgcggcgtg aatgatgttg ctctcaatca tcatgttctg gtagatgaaa
    44461 tccgccggat aggtgttgtt ggcaacaatg ccgcccactt tcgccgccgc cagatagacc
    44521 tggtcaatgc gttcgctggc aaagaaatca tgcacggcgc ggctgtccag caggttcagt
    44581 tcatcgcggg tacgtaatac cagctccaca tcaccgcgct gttcgagctg ccgcttgatg
    44641 gcagaaccga ccatcccaca atggccagcg ataaaaatgc gttgtttact catgcttatg
    44701 actccagcgc gatcgccacg tcgtagccgt gagatttcag cagagagtgt tttttcgccg
    44761 cttcgaggtc attagccacc atttcagaca ccatctctct gagggtgatt tccggtttcc
    44821 agcccagttt ttcgtgcgct ttggtcgggt cgccgagcag cgtttcaact tctgccggac
    44881 ggaagtaacg cggatcaacg gcgataatca catcacccgg tttaacgccc ggcgcgtcat
    44941 gcccggtgac ggaaaccaca atgcccttct cttcaacacc cgtgccttca aagcgcagtt
    45001 tgatgcccag ctgtgctgcc gccatttcca cgaactgacg tacggagtac tgaacgccgg
    45061 tcgcgataac gaaatcttcc ggctgttcct gttgcagcat catccactgc atttttacgt
    45121 agtctttggc gtggccccag tcacgcaggg aatccatatt gccgaggtac aggcacgact
    45181 ccagcccctg ggcgatgttg gcgattgcgc gggtgatttt gcgggtaacg aaggtttcgc
    45241 cgcggcgcgg ggattcatgg ttgaagagaa ttccgttaca agcgtacatg ccgtaggatt
    45301 cgcggtagtt aacggtgatc cagtaggcgt acagtttggc gaccgcatac ggagatcgcg
    45361 ggtagaacgg cgtggtttct ttctgcggaa tttcctgcac cagaccatac agttcagagg
    45421 tggaagcctg atagaaacga gttttctttt ccagaccgag gaagcggatc gcctccagca
    45481 ggcgcagcgt acccatcgcg tcgacgtcag cggtatattc tggtgactca aaagagaccg
    45541 caacgtggct cattgcgccc aggttgtaca cttcatccgg ctgtacttca cgcaaaatgc
    45601 gcgtcaggtt agaggtatca ctcaggtcgc cataatgcag atggaatttc gggttgcagg
    45661 tgtgcggatc ctgataaatg tgatccacgc gctcggtgtt gaatgacgat gcgcgacgct
    45721 taataccatg cacctcgtaa cctttttcca gcagaaactc tgccaggtaa gaaccgtctt
    45781 gtccggttac accggtgatg agagcgactt ttgacatgtc ttattcctct gtatttttga
    45841 atttattcag tttcaacgcg ttcgcgtatc accactgcgg gattcccccg gcaaaccaca
    45901 tttgccggaa gcgatttaaa aacactgctt cgtgcaccca cgacggtgcc gtcgccgatt
    45961 gtgacgccta gggcaacaaa gacatcggtt gccagccagc atttctcgcc aatcacaata
    46021 ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt ggctaccggt gcataaataa
    46081 cttttttgcg atatcaccga atgtgcgcca atggttattt caccgagggt atataaattg
    46141 acgtcatcgc cgacccacgc gtaatcacct aaggttaatt tccacggata ggtaattttt
    46201 actgacggac gaataactac gttttttcct atttttgctc cgaataaacg taataaaaaa
    46261 gcccgccagc gatacaatac ttgtggcgac caggcaaata ttgttgcctg tactgcccac
    46321 cataattgca ctttaatagc gttgccgccc cggaaccctt tcggcaccga gaaaccgctt
    46381 aaatcttgca ttggttttcc ttatattcag actttgttat ataaggcttt agtcttacct
    46441 gtcgtacgtt ggcgtaaatg ccaggataat tctgcccaga agccaggtac atgtaatatc
    46501 tgccgttgga cttttttggc gtcagcacac aattccatat tattggtggt tgatacccca
    46561 cccatagaaa attcagacac caggccattg agttttttaa atgcataacc ggctttatac
    46621 attttagctg ccagcgcgta atcagaagaa actttatatt ccaggtcata acgccatttt
    46681 ttcaagccgg ataccgggaa aaatatcgcc tgatggctgg cgggcaggct gtgataaata
    46741 taccagcccg gtttggcgct acgtttaatt ttatgcccgt cgccaaaatc cagcagcgca
    46801 tcgccagtga tcatcacgtt atctttttgc atttttaact tacggacaaa atttgcggca
    46861 tcctgatgaa aaatatcgcc cgagttgaga aacaacgcga acttgccttg tgccatcgca
    46921 atgcccttgt tcatggcgtc gtagataccg ttatctggct cgctgacaaa gcgtaggtta
    46981 tagataccat tgagattttc cagatactca cgggtagcgt cgttggaacc gccatcgaca
    47041 acaatccatt cgaagctgat atcgtccgcc tgcgccagat gcaccagcga ggcatgtgtt
    47101 ttgactatcc cttcgaggtt acgaaacgcg acagtgatta tgctaagcaa catgttctta
    47161 ttcttacctc gtaatattta acgcttttcg caaaataaac ggacagacga ttaaaaatgc
    47221 atattccggg ctaaatatcg aaccggtaaa aaacagcgat accggagtaa aaagatataa
    47281 ttgcacgcga aaattgcggt tatcactaaa agcgttgatc atcattttta tcactttccc
    47341 catgtaccac agcgataaaa acaccgcgaa ccaggaaaaa taaataatca gcagatacaa
    47401 accattgtct atggtttttc cgacatccgc accgttaaat attccgaatg atgcaacata
    47461 ttcataaagt gagccaaatc tgactacacc gtcaatatgg gtcaaagaat aaccgaccat
    47521 caccagcgga ccgacaatac gataatacga tgacgatcct tccgtaccta aatcgcccag
    47581 acgggtggaa atataaggaa acgcgattac cacaccaacc aggaatacag ccagagaaat
    47641 caatgctaac ggtaattttt tcttaatcac ctctttattc agatattgaa atgcccactc
    47701 cagcaaataa aacaggataa aggtcataac ccctgaaaac gatccggata atattatccc
    47761 tgcgagaatc atagcatctg ttttaggcgt tttgatacca aactgtttga tgctgagcca
    47821 aattgagatt aatgccagag cgaaaaatgc cggttcgaaa taaagtgctg tcgtgcgctt
    47881 gccgccgaat ttaatgaaat tcagcacata gctgttgctg taaatcagat atttcgaaat
    47941 cggctccata atactgctgc caccggtgag gataatttgc gccatctcca ccgccgccag
    48001 cgccaccacc agtccgacca ccagataaaa gaaacgtaat atcttacgat ggttgtgtgg
    48061 cgaaattgtt ttaaaacgaa tactccacac catgccaata atgatcacaa tatagacaaa
    48121 cagcatagtt gaagtgacgt atttaccggc atccaacgac tgaccaaaca ggtagttaaa
    48181 cgccgtgagt cccgcgccaa tccctaaggc aatcatcaat tttttaatgc tgatgcgttc
    48241 taaaaacagc agtaacaaga caggtaaaaa agtgacgatg gtgatgggga aactttcgcc
    48301 aagctgggcg attttgacgt taaccagcag gtagatcagt ggcagcagca ggtagctaca
    48361 gattctgata gaagttgaca tactcctcca gcatctgttg tccactgtag gcggcgcgac
    48421 tgcgttggct gaactcagcc agcgtggtac caaatatcgc ctgcgcgatt tccggtttgc
    48481 ttaactgtac cagttgcagc acctcttctt cactgacggt tttaccaccg gatttttgca
    48541 gcacttcccg cgccgcatcg ctatgggtgg caatcaccgg cacgccaatc gatagcgcct
    48601 cacacaaaat cagcgggtag ttatcgacac gagaactgaa caccagcgca tccatctgat
    48661 tgagcgcgct catcaacttg cgcttgtcag tttcaaagcc gtgattaacc acgttgccag
    48721 cggtgaacgg cgagaactta ccaaaggtat gcagttcgat tttgtcgccc agcgccatca
    48781 tctcacgcac cagttgctgg ttagttttgc cgtcgtaacg caggtcatgc gccaccaccg
    48841 cgattttcgg cttgccctga gtttcgcgca ccggaggcag atctgccaga atcgcttcgg
    48901 ttgccatatc aatgccatta ttgataatcc ggcaacgccc tggcccgtac agactattga
    48961 aagcgtcagc cacatgctgg ctgggggaaa taaactgaca gcccagcgcc agcatctcgc
    49021 ggaataactg gcgtttgccc gccaccagct ggtgcgcgcg atcaatcttc accggcggat
    49081 agttatttaa ggtcgggcat ttctggcagc ccgttttcca gccttcgcaa ccgtcggtaa
    49141 aggcgcagcg tccggttaca ctccagtggt cgtgcagcgt ccagaccagg gtgacatccg
    49201 gtttatggtt tttcactttt tcgcaaaagc gcaccacgct gttaagattc aaccagtagc
    49261 tgtgcagcac atgaaaatgc aggaccaccg gacccggtgt gcgagtaatg gtgcgatata
    49321 actcattgaa attgccgaac agatcgcgat taaacagacg aaacaacgca atgttcgcca
    49381 tcgcggtcat ccgcggcgta tgtttgatga cctgcggata gttctgatgg ctgacgctct
    49441 ctttgccgcc tttgccgtag ccgtagacaa aatgtgacgc cagcccctgt tgcagcgcac
    49501 gctggtggag atctaacgcc acacctgctg ccccgccttc cgccagtcgc acattaaatt
    49561 gcagaatatt catttaatta ccttcactcg cgctttttct cccaccacca gcgcgttgtc
    49621 cgggacagag tcgagcacca cgctgcccgc acccacggtg acgttgttac caagcgtgat
    49681 atcaccaaga ataatgacgt tggcaccgag ttcgacgccg ttgccaatgt gtggacatgc
    49741 catgttatcg gcaccccgat tgccgatagt gacgccgtgg cgaatggtga aatcatcccc
    49801 cgccaccacg tttttattga tcactacggc gtaaccgtga tggatagtaa agcggcggcc
    49861 aatggtcgcg gcggcctgga tttcataacc gaaaaagcat tcggtgataa tgcgatacag
    49921 caccagcagc ggggccgccc acagattgtt gaggacgttc tttttgcgcc acaccgaaca
    49981 aaaatgagcg acgcgatagg caagtaccat gcagcacggg cgtaaactcc agctgttggc
    50041 gcgcagatct tccagcatct ttagcgcccc cgaataccat cagccagacg tttgccgtta
    50101 cgcaccgaca gcagcgtcaa caaggtgcgc caggtcatac gtttattgcg gatctggtag
    50161 agggtaaaca gctgatattt tttactggcg cggtcgaatt tgtctttgtg cttgcggtaa
    50221 aagtggaagt atccggagaa ttttttcggt gacgaggtga tctgcatttc gccgtgattg
    50281 atatgcagga tctgcgtcgc ctcttccact ttccacggtt cgccgtactc caccaccatc
    50341 cgcaggaata tgtcgtaatc ctgtgctgct ttcagttcgg tatcgaacaa acactctttg
    50401 aaacgccatg cccaggtaaa gacctgattg ccaatgatat tgcgtttata gaacaggcgg
    50461 cgtgagtacg gcgatttggg atacagtggt aagctcgccg gttgggaata aacttctccc
    50521 tggcaaacgt agtcgttagc gtacaaaaag gcgtgtgtga ccagttgctg tttatgggcg
    50581 aggaagacgc tcaggcggtt gggcgtccat tcatcatcat catcgatccc ggtgatgtat
    50641 tccccttgcg ccagcataat cgcctggtta cgcaccgcgc acgccccgct gttaatgtcg
    50701 ttgtggatgt aagtgatacg cggatcgttg agggcagtga cgtactgttg cagttgttcc
    50761 caggaggtgg agcaatcatc cacgatgatc atctcccagt tgctgtagtc ctggcgcagg
    50821 accgatttta tcgcccgaat cgccagttgt tggcggttcc aagttggcat atagattgag
    50881 atcagcggat tgtctttcat aggttgttct cccggcttat gagctggttt gcttgccgga
    50941 tgcggcggag cgccttatcc ggcctacggg gcggtgcgaa tgcaggcgtc gttatttcgc
    51001 atccgactta tattcgtatt cgtaatagcc ataatcctga tacgcgctgg cgcggcggaa
    51061 gatggagttc agaatcaccc ctttcaccgg aataccgttt tgctcaaagc ggctcagact
    51121 ggtttccact tctttcaatg tgttgaccgc ataacgcgcc accattaacg tggttccgac
    51181 atgacgacca acaattgccg catcggtcac tgccagaatc ggcggcgtat caatcaacac
    51241 caggtcatag tttttactcg cccagttcac cagttcggca aagcgttcgc tcattaacag
    51301 ttcagaagga tttggcggca cctgaccgcg cgggatcagg tcaaatttgg caatagaggt
    51361 cggtttagca gcggtagtaa tatcgccctg accaatcaga atctccgaca gcccattgac
    51421 attattggtg cccaacagct cgtgggtgta gcctttgcgc atatcgcagt cgatcaacaa
    51481 cacgcgttta ttggtctggc tgatcaccgc cgccaggttg gcgcagacaa aggttttacc
    51541 gattgacggg ctacccccgg tcatcatcaa cacattgttc tgcgcctgca tcatcgcgaa
    51601 gtgcagactg gtacgcagac tacggatggc ttcaatcgcc agatcggttg gattccccac
    51661 tgccagtagc tggctctgtt tatagcgttt aacccctttg atggttttga cgctatcgcg
    51721 cgctttctgc cactccgaca gcgggatgct ggcatagacg ctgattccgt gttcttccag
    51781 cacttgcggg ctttcgatgc cgcgattaaa caacgagcgc agcagcacac ccacgataga
    51841 gagcatcagg ccaaggataa tcgcgccgag gataatcagc cctttcttcg gttttagcac
    51901 gccaggctgg gtgattgccg ggtcaataat gcgcacatcg ccgacggtgc tggcttcggt
    51961 gattttcagc tcctgctctt tattcagcag ttgcatatag acctgctgac cagactcgac
    52021 atcgcgggtc agacggacaa tttcctgctg ggttttcggc atcgccgtta cgcgaccgtt
    52081 aagtttggct ttctcttctt ccagcgcctg acgtttctcc agcagcgtgc ggtacgccgg
    52141 gtgaactttg gtgtacagct tggagatttc cgcctcttta aaggtcagtt cgttcaactg
    52201 cgcgtcgatg ttcaccatcg aatcgagcac cgctttcgct tcaagcggca gatcaacaga
    52261 atctttatcc tgacggaagg cattcagttt gttttcggca acatcgaggc ggctacgtac
    52321 ttccggtaac tgttgcgcga ggaaagcgag gcttttcgac gcttccgccg atttgcgctc
    52381 aatattttgt tcctgatagt tacgagcgat gctgttaaga atgtcgcgga tctgttcgcg
    52441 atcttcaccg gtataagtca ggctcagtac gcctgcgtct ttgccgttct ccgttaccgt
    52501 caggctgttt tgcagttggt tgatcatccc caacgtggag tatttggtga cggtaaactc
    52561 actgcccggg cgggtgtgaa tggcttcaac catcagcgtg acgccttctt ttttcagcat
    52621 ctggcccgct tgcccacggg cgctaaagcc gccatcactg ctcagggtgt agtttttgtc
    52681 atccagcaca ttaagcgtaa acacctgatc cgccatctct ttcgggcggt taaaggtagt
    52741 cactttcacc gtctcgttct gacgccccat caggcgatcc cagcccgccc cgaaaatcgg
    52801 gaacgtgttt ttgctcaccg caatatcgag gtcgagatcg tccaccgttt taccaagcac
    52861 cagacgcgaa cgaatcaact ggatctcggc gtccgatgca ggcggtttgt tggctaatgc
    52921 cgagccgata tcctgcacca gcgaattgcc gctgttttgc tcgatttgta ccagtgcgtc
    52981 ggcactataa atcggcgtgg cgaagaaagt gtaaaccacg gcacagaggg caaatacggc
    53041 ggtgatgcca atcacccacc agcgcgcttc aatgacggtg ccgaccaggc gaccaatatc
    53101 gatttcatca ctgcccgtta ccggagcggc atgttgtttt actttttctg tcattcttat
    53161 acctgctctg cgttcaatgc ctgcgcccac tggcgggcag accgttcaag taatgtgtac
    53221 accgctgcaa acgtttcccg gcttttgcga tacggatcgg ggatttcaca ttcgttatcc
    53281 cagtgaccaa acagcatcac tttgccgcgc atctccggtg ccatctcgca taagcgttcg
    53341 atatggcgct tatccatggt caaaatcagg tcatagttgc gacacaggcg gcggctgatt
    53401 tgtcgtgcac agtggccttc cagagacagt tgatgttctg cggcgacgct gatagcggta
    53461 ggatcagcgc ccttaccgac cagcgcgccg agtccagcgg actccacttt cagttccggg
    53521 tgataacgtt gcagtaagcg ttccgccatc ggggaacggc aaatattgcc gacacagaca
    53581 actaagatgt tgttaaacat gacgattacc agttatgaat gtcgctggct gtatccgtca
    53641 tgtaacggac accgctaata gttggcagca actgattgat cagacggttc cagcgggaaa
    53701 ccggggcggt ggtgacatac accacgtcat aaggttgcag gcggaattct gtcgccatca
    53761 ccagcgacgt cgcatcggac atatccagct ggtagatatt ggcaatcttg ccgttacgcc
    53821 cgccctcgcc tttcagcgga cgaatgacaa agatgccgct ggcgttggag gtggtcatgt
    53881 cgataccttc ggcattgccc agagcttcag tcagggtcat gccgctaaag tccattttca
    53941 gggtgctctg tttcttcact tcacccatca caaatacttt cagatcatca ttacgcggca
    54001 cgtagagaat atcgccggga taaagcagtc ggttctgatt gaggtcgccg ttttgcatca
    54061 gcgcctgtaa agaaatgcgt tcttcacgac cattgtgtgt tagcaccacg ttgcgccagt
    54121 cagcggtgtc ggtcaggcca cctgcggcgt tgatggcgtc gagaatggtc agtggtacgt
    54181 tggtgatcgc ctgttgaccg gatttattca cctgacctga gatataggcc ttttgtgagc
    54241 ggaaggcggc gatattaacg tccacctgcg ggtcagcgat gtacgtcgct aagcgcccgg
    54301 taatatcact gcggatttca gcgagcgttt tcccgactac gtgaaccttg ccgatatacg
    54361 ggtaaaacat agtgccgtca ggctgtaccc agttgccggt gtcgctggag ctgcggtact
    54421 gaccagctgg cgtggtgagt tccgggtgat cccagacggt gacattaaga acgtcccccg
    54481 gcccgacgcg atactggtaa ttcgcgatct cgctttccag cgtcatattg gggcgcgcta
    54541 cattcgggcg tgggcgtaat tggtcaatca ggcgcggggt cagcggataa acattcacca
    54601 ttttgtcgag atcgaaatca gcgtcctgct gtttgatgac gtctttgccc atcgtcgaca
    54661 tattgctgcc cggaagtact gtgcaaccgc ttatcaaggt tactgacacc aataatggca
    54721 tcaatttcat tttggatttc atcattgttt atttatcact ttggcagagt aattatcctg
    54781 tgcactatta atagcaatgt cgccatgcac atttaccttg cagttaattg aataaaaatt
    54841 taactggcat cagtcctaaa aaaattgatt tcatccgcag gctattgaca gaataattca
    54901 gactggtctt tcaggcatcc agacacgcta ccgcccctgg ctttttagct accaatacac
    54961 tgattgagct taatttttca aaccctctct gcatacaatc gttgatgaga aagggttatt
    55021 gcggaaatta acttccgaat ataaggtgac attatggtaa ttgaatattg gctctccaat
    55081 aatgcaggag gaagtgttac agctaacgga atagcaggca aggtaacaat tcggcaattg
    55141 gctattttta agaattatat taagtgtcat tcaatatggt ttttaggagt ttctttaggt
    55201 tgacaatatt taatattgcg tctccatatg cgatatttct taaataatgc tttattatta
    55261 ccgcttttaa ttcagggata atgtgaggtt attaccctca aataatatga gataaatagt
    55321 gctgcaacat tgcatttttg ccccgattta tccatgatcg aattgtgaca tttgtcatac
    55381 aacgaatagg ttttgtactt actatggaat ggattgccga tccgtctatc tgggccgggt
    55441 taatcacgct gattgtgatc gaactggtcc tcggcattga taacctggtc tttattgcca
    55501 tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc acgggttacc gggctgctgc
    55561 tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc ctggctggtc accctgactc
    55621 aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg cgacttaatc atgctgtttg
    55681 gtggtttctt cctgttgttc aaagccacga tggagctgaa cgaacggctg gaagggaaag
    55741 acagcaataa tcccacacaa cgcaaaggtg cgaagttctg gggcgtggtg acgcaaattg
    55801 tggtactgga cgccattttc tcactcgact cagtgattac cgccgtcggg atggtcgacc
    55861 atttactggt catgatggcc gccgtggtta tcgctatcag cctgatgttg atggccagca
    55921 agccgttaac gcaatttgtt aacagtcacc cgaccatcgt tattctctgc ttaagcttcc
    55981 tgttgatgat tggctttagc ctggtggcag aaggtttcag cttcgtcatt ccgaaaggct
    56041 acctgtacgc tgccattggt ttctcagtga tgatcgaggc actcaatcag ttggctatct
    56101 ttaaccggcg acgttttctt tccgctaatc agacgctacg ccagcggact accgaagcgg
    56161 taatgcgcct gcttagcggg caaaaagaag atgcggaact ggacgccgaa accgcgtcca
    56221 tgctggtgga tcatggaaac cagcagatct ttaatccgca ggaacggcgg atgattgagc
    56281 gggtacttaa tcttaaccag cgtaccgtca gcagcattat gacgtcgcgc cacgatattg
    56341 agcatatcga tctcaacgcg ccggaagaag aaatccgcca gttgctggag cgaaatcagc
    56401 atacgcggct ggtggttacc gacggtgatg acgcagaaga tttgctcggt gttgttcacg
    56461 ttatcgacct gctacaacag tcactgcgcg gcgaaccgct caacctgcgg gtgttgattc
    56521 gccagccgct ggtgttcccg gaaaccttgc cgttgttacc agccctggag cagttccgta
    56581 atgcccgcac gcactttgct tttgtggtgg atgagtttgg ctcggtggaa gggattgtga
    56641 cattaagtga cgtcactgaa accattgccg gtaacttacc gaacgaagtg gaagagatcg
    56701 acgcccgcca tgatattcag aagaatgcgg acggttcctg gacggcgaat ggtcatatgc
    56761 cgctggagga tctggtgcaa tatgtgccgc tgccgctgga tgaaaaacgt gaatatcaca
    56821 ccattgccgg gctgttgatg gagtatttgc agcgtattcc aaaacctggc gaagaagttc
    56881 aggtggggga ttatttgctt aaaacgttgc aggtggaaag ccatcgcgtg cagaaggtgc
    56941 agattatacc gctgcgtaag gatggcgaga tggagtacga ggtgtgatgg tatcggccat
    57001 tttacccaat gctgcgtaac gccttataac ttcagcaatg aagcgttgtc tgtggtaaac
    57061 ggacatcaca gcggtggtgt gggtccggct gtaaatcgcc gaatcgtttc ctgtaggctg
    57121 gggcagccca catggatgtg ggctgagggc gcgttttaca gggatgttac ctcgcgcccg
    57181 tacccgatag ccgaaaggaa taagatgagg gaaccgcgcc agcggcgatt taccgccggg
    57241 agcccgggtt gccagggtgg tggcgattga gccaccctgg cacgttcacc ggcttgagcg
    57301 ttccatagta gcgaggaaca tcaagtgaac ggaacagcct tcgatcttac atattcagaa
    57361 tataatagtg ccgccgcgaa cgcctgatgc gacgctttac gcgtcttatc aggcctacag
    57421 gacaggtcac ctggtaacct acatcttctc cagcaacttc ttcacatctt tgccattgcg
    57481 ggaatcttta ttccgctcgg cccagtcatt caggcgacgt ttcgcttcgt cctgtagatg
    57541 tttgcgcagt aactgatcca cttgcaggct gtaattgagt tgctgccagt tgccataaac
    57601 ccgcagcggt actggcgttt ctttgaggaa atcaatcagt ttgctttccc cgttccagcc
    57661 acccacgacc cgaatatcaa actgggtgtc gcaggtttga tctgccagat tcaacatgcc
    57721 ttcccctgtc agcgccagca caggcgattg accttgcatg tcgtttaacg tcacgacgcc
    57781 atccttcaac gtcaaatcgg tggtaaagcg gtcaagccgc gtcacgttat cgaagttttc
    57841 agcggccttc acatcaccgc cattacgctc taccgcttgc tgaatcatct gctggaagtt
    57901 catcccttcc atacgtgtgt cggtcatctc aacatgcgcc tgcccttgcc agttgtggcg
    57961 gaatgcgtcg gcatctatgt cagcaccgga gaaatcacca gccagtgaca tttttccggt
    58021 caacgaaatc ggatagttaa acgccttcag aatggtacca atctcaacgt tctccagccg
    58081 tggctggaag tttatccgcg gatttattga tgtcgcgtcc agcgtgcccg gcagtgaaac
    58141 ctttccaccg ttaagcttgc cctgcagttg agtaatttcc agcaaacccg acttgttggt
    58201 catttgcgtg gcaacatctg taaaattcat tccgcgccag cgcacgttac tggcctgcaa
    58261 caaaatatca gctgtaaagc cttgcagtcc ctgataggca ggttcatcaa tacgcgaaga
    58321 aatgaccggg cgcggcaacg ttgattggct ctgcccctgc tgcgcggcac cgttttcacc
    58381 attcgcggtt tcattaagcg ggatgaggtt gtcaagattc agttgcggga attgcagtct
    58441 cagctgccat tccggtttct ctgtcagcgt gacctgtgct tgcccgctca gcgtactgtc
    58501 attggcggtc aaactaattt ggttaaatga aaggcgttta tgtgactcct gccactggga
    58561 ctggaaactc ccctgcccct gaataccttg ttttggtaaa tcggcaccct gcaactgcca
    58621 gttaatttgt tcaatagccg ctgttaaatc atgcggataa tcagaagcat ctaccgtacc
    58681 gttaagggat atcgtgagat cgcgctgatc gcgattaacc cgcccggaga actcaaatga
    58741 gccacgatgt tggggatctt gttccatttg caggcggata ttgcggattg tcacctgctc
    58801 gtcatcttca tgctggaaca ccagaacgct gtccgccacc ttaagactgg atatatcaaa
    58861 cgaccatccg cgatcgtctg acagatccgg caaggtattg tcgcgcggtg caaccggagc
    58921 gtcttcactg cgcaccgctt ccgtctgcgg cgtcagttgg atcactgccc cttttagcat
    58981 cacctgctta acgctcagtt gatgactcag tagtggtaaa agcgccacgt ccagacgcat
    59041 gttgtcggcg cgaaccagtg gctggcttgc gccctgggcg gtgagagaca ttcgcccgga
    59101 gaggatacta agctgcggcc agacgtgcca acgcagtggc ccgtcgagct gcaattgata
    59161 accgctacgt gcagcaactt gcttgaccat atagtcgcgg aaatcattcg gattcaccag
    59221 caacactaac gcagataacc cggccaccag cacgaccagg agtatcatca gcgtcgtcag
    59281 aaatcgtctc atggtatcct caatgggcct gaattagtct ttatcgattc ggctggctac
    59341 cgcgccctgc tggttgcgat atttcgcatc ttcacggcgg ttgtaaggtc gcgccgccgg
    59401 gccggaaagc ggctcaaagc tcagcgcacc aattaacatg cccggacgca gcgccagcgg
    59461 cagcttaccg gagttgtaga actccagcac aatgcaacca gaccagcccg gatcgatacg
    59521 gtgcgcggtg acgtgcacca tcagccccag acgcgccaaa gaggaacgcc cgtccagcca
    59581 gcccaccaga tcggctggca gcgtcaccga ctccagcgtc accgccagcg ccagctctcc
    59641 tgggtgaaga tagaacgcct cgccctcgtc gagaacgatc tcatcgctca tcacgcggtc
    59701 aagcgcggcg ctcacttcat ctttgggacc gctcagatcg ataaacgctg ccgtgtgacc
    59761 acggaaggta cgaaatttat tgcccaggcg tacatccacc gtcgcgccgt taatacgctc
    59821 cactggcgga cgtgggttga tcgacaaacg gccttcatca agccaggctt caatatctcg
    59881 gtcacacaga cgcatggcat tttctccttt cgcgcatcac tcccttaacg ccaattacgt
    59941 caagggcata ccagcttatc actgaacggt acacaattta tcaagcttat tcaaagaact
    60001 gacttatttt cgctttcaat atatcgatcg cgatgcggtt tttcccgccg cgcggcacga
    60061 taatgtccgc atattgttta gaaggctcaa tgaattgcag gaacatcggg cgcacggttt
    60121 tttgatattg cgccatcacg gaatccattg aacgcccacg ctcgttaacg tcacgcttga
    60181 tgcggcgcat caggcagata tccagcgggg tatcaacgaa aatggagaag ttaagttcgt
    60241 cacgcaaacg cgcatccgtc agcaacaaaa tgccttcgag aatgatgacc ttcttcggct
    60301 caaccgtcac cgtttctttc atacgcgtat gttcaacata gctgtaaacc ggcaggtcaa
    60361 ttgccgagcc gcgtttcaac gcttgtaaat gctcaagcaa caggctgtga tccatcgcgc
    60421 tgggatggtc gtagttggtc ttaacgcgtt cttccatcga cagatggctt tgatctttgt
    60481 aatagcagtc ttcgggaatt acgccgatgt gttcatcacc gacttgctca cgtaattcac
    60541 gataaagggt actggcaata agactcttgc cggaagccga tgcgccagcg ataccgataa
    60601 tgacgcactg atgagactga tcagtcatat atttagcgac ctgattaacc tagatgttag
    60661 gaagggggcg acgaagcgcc aaacgcggca attataggga tttcatccgt ctgataccag
    60721 tcgaatagcg ttgccgcgcg ctcagagtta attgttgaca aagaattccc ggtggcaaat
    60781 tacgttgatc agttttatgt aaggtaaaaa atgttatacg cagttgcgca aattatccgc
    60841 ctttacgtca ctttatgagc aattcgcata taaaatgtaa aacttttgta ctagcataaa
    60901 cacagaaacg aatactggcg acctggcctt gcggataaag cggtaataag caaacaatca
    60961 cagcatgtat taattgccct gccccacccg ctgcttcacc tggtcagttt aggtttagtc
    61021 tcgtttatct ttacccttgt ctcgcttgag ctttcgcagt ttggcaccca actcgcccca
    61081 ctgtggttcc cgacgtccat catgatggtg gcgttttatc gccatgccgg gcgcatgtgg
    61141 ccgggaattg cgctgagctg ttcgctggga aatatcgccg catccatcct gcttttttcc
    61201 acctgctcgc tgaacatgac ctggacgacc atcaatattg ttgaagccgt ggtcggggca
    61261 gtgctgctac gtaaattgct gccgtggtat aaccccttgc aaaatctggc tgactggctg
    61321 cgtctggcat tcggcagcgc cattgttccg cctct
//
LOCUS       DADKXM010000011        55236 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.011, whole genome shotgun sequence.
ACCESSION   DADKXM010000011 DADKXM010000000
VERSION     DADKXM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.011"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..534
                     /locus_tag="JQR05_000672"
     CDS             <1..534
                     /locus_tag="JQR05_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322906.1"
                     /translation="IALAACADKSVDIQTPAPAANTSISATQQPAIQQPNVSGTVWIR
                     QKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVLPFNPADVQPNAR
                     ILLSAAINVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQASGGATTTVPST
                     SPTQVNPSSAVPAPTQY"
     gene            complement(565..876)
                     /locus_tag="JQR05_000673"
     CDS             complement(565..876)
                     /locus_tag="JQR05_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY5322907.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            1039..1135
                     /gene="ffs"
                     /locus_tag="JQR05_000674"
     ncRNA           1039..1135
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JQR05_000674"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1255..1608
                     /locus_tag="JQR05_000675"
     CDS             1255..1608
                     /locus_tag="JQR05_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY5322908.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1650..3200)
                     /gene="pdeB"
                     /locus_tag="JQR05_000676"
     CDS             complement(1650..3200)
                     /gene="pdeB"
                     /locus_tag="JQR05_000676"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY5322909.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PLCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3364..3834)
                     /locus_tag="JQR05_000677"
     CDS             complement(3364..3834)
                     /locus_tag="JQR05_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322910.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3950..4501)
                     /gene="maa"
                     /locus_tag="JQR05_000678"
     CDS             complement(3950..4501)
                     /gene="maa"
                     /locus_tag="JQR05_000678"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY5322911.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4673..4891)
                     /gene="hha"
                     /locus_tag="JQR05_000679"
     CDS             complement(4673..4891)
                     /gene="hha"
                     /locus_tag="JQR05_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY5322912.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4917..5291)
                     /gene="tomB"
                     /locus_tag="JQR05_000680"
     CDS             complement(4917..5291)
                     /gene="tomB"
                     /locus_tag="JQR05_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY5322913.1"
                     /translation="MDEYSPKRHDIAQLKFLYETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     LGNRLFRCFVNATKENPASLSC"
     gene            complement(5837..8986)
                     /locus_tag="JQR05_000681"
     CDS             complement(5837..8986)
                     /locus_tag="JQR05_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY5322914.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(9009..10202)
                     /gene="acrA"
                     /locus_tag="JQR05_000682"
     CDS             complement(9009..10202)
                     /gene="acrA"
                     /locus_tag="JQR05_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY5322915.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLIQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            10344..10991
                     /gene="acrR"
                     /locus_tag="JQR05_000683"
     CDS             10344..10991
                     /gene="acrR"
                     /locus_tag="JQR05_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY5322916.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11119..14481
                     /gene="mscK"
                     /locus_tag="JQR05_000684"
     CDS             11119..14481
                     /gene="mscK"
                     /locus_tag="JQR05_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY5322917.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14693..14854)
                     /gene="rsmS"
                     /locus_tag="JQR05_000685"
     CDS             complement(14693..14854)
                     /gene="rsmS"
                     /locus_tag="JQR05_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY5322918.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14868..15395)
                     /gene="priC"
                     /locus_tag="JQR05_000686"
     CDS             complement(14868..15395)
                     /gene="priC"
                     /locus_tag="JQR05_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY5322919.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            15465..15842
                     /locus_tag="JQR05_000687"
     CDS             15465..15842
                     /locus_tag="JQR05_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY5322920.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15995..16546
                     /gene="apt"
                     /locus_tag="JQR05_000688"
     CDS             15995..16546
                     /gene="apt"
                     /locus_tag="JQR05_000688"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY5322921.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16675..18600
                     /gene="dnaX"
                     /locus_tag="JQR05_000689"
     CDS             16675..18600
                     /gene="dnaX"
                     /locus_tag="JQR05_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY5322922.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRELLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18653..18982
                     /locus_tag="JQR05_000690"
     CDS             18653..18982
                     /locus_tag="JQR05_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY5322923.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     NINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18982..19587
                     /gene="recR"
                     /locus_tag="JQR05_000691"
     CDS             18982..19587
                     /gene="recR"
                     /locus_tag="JQR05_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY5322924.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19697..21571
                     /gene="htpG"
                     /locus_tag="JQR05_000692"
     CDS             19697..21571
                     /gene="htpG"
                     /locus_tag="JQR05_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY5322925.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21752..22396
                     /gene="adk"
                     /locus_tag="JQR05_000693"
     CDS             21752..22396
                     /gene="adk"
                     /locus_tag="JQR05_000693"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY5322926.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            22528..23490
                     /gene="hemH"
                     /locus_tag="JQR05_000694"
     CDS             22528..23490
                     /gene="hemH"
                     /locus_tag="JQR05_000694"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY5322927.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDEALKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(23487..24446)
                     /gene="aes"
                     /locus_tag="JQR05_000695"
     CDS             complement(23487..24446)
                     /gene="aes"
                     /locus_tag="JQR05_000695"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY5322928.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24598..25902
                     /locus_tag="JQR05_000696"
     CDS             24598..25902
                     /locus_tag="JQR05_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY5322929.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEHEDSLEESYWDR"
     gene            complement(26035..27711)
                     /locus_tag="JQR05_000697"
     CDS             complement(26035..27711)
                     /locus_tag="JQR05_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY5322930.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27949..29169)
                     /locus_tag="JQR05_000698"
     CDS             complement(27949..29169)
                     /locus_tag="JQR05_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5322931.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWMGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            29387..31039
                     /locus_tag="JQR05_000699"
     CDS             29387..31039
                     /locus_tag="JQR05_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY5322932.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEYGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(31076..31555)
                     /gene="ybaK"
                     /locus_tag="JQR05_000700"
     CDS             complement(31076..31555)
                     /gene="ybaK"
                     /locus_tag="JQR05_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY5322933.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31759..32553)
                     /locus_tag="JQR05_000701"
     CDS             complement(31759..32553)
                     /locus_tag="JQR05_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322934.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32691..33032
                     /locus_tag="JQR05_000702"
     CDS             32691..33032
                     /locus_tag="JQR05_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY5322935.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(33247..35751)
                     /gene="copA"
                     /locus_tag="JQR05_000703"
     CDS             complement(33247..35751)
                     /gene="copA"
                     /locus_tag="JQR05_000703"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY5322936.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITRGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            36013..36945
                     /gene="glsA"
                     /locus_tag="JQR05_000704"
     CDS             36013..36945
                     /gene="glsA"
                     /locus_tag="JQR05_000704"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY5322937.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36948..38240
                     /locus_tag="JQR05_000705"
     CDS             36948..38240
                     /locus_tag="JQR05_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5322938.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPCNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            38365..38772
                     /locus_tag="JQR05_000706"
     CDS             38365..38772
                     /locus_tag="JQR05_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY5322939.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38773..39231)
                     /locus_tag="JQR05_000707"
     CDS             complement(38773..39231)
                     /locus_tag="JQR05_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY5322940.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(39228..40145)
                     /locus_tag="JQR05_000708"
     CDS             complement(39228..40145)
                     /locus_tag="JQR05_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY5322941.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            40291..40968
                     /gene="fetA"
                     /locus_tag="JQR05_000709"
     CDS             40291..40968
                     /gene="fetA"
                     /locus_tag="JQR05_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY5322942.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40955..41734
                     /gene="fetB"
                     /locus_tag="JQR05_000710"
     CDS             40955..41734
                     /gene="fetB"
                     /locus_tag="JQR05_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY5322943.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41797..42651)
                     /gene="cnoX"
                     /locus_tag="JQR05_000711"
     CDS             complement(41797..42651)
                     /gene="cnoX"
                     /locus_tag="JQR05_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY5322944.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42712..43521)
                     /gene="ybbO"
                     /locus_tag="JQR05_000712"
     CDS             complement(42712..43521)
                     /gene="ybbO"
                     /locus_tag="JQR05_000712"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY5322945.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(43511..44134)
                     /gene="tesA"
                     /locus_tag="JQR05_000713"
     CDS             complement(43511..44134)
                     /gene="tesA"
                     /locus_tag="JQR05_000713"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY5322946.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            44105..44791
                     /locus_tag="JQR05_000714"
     CDS             44105..44791
                     /locus_tag="JQR05_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5322947.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44788..47202
                     /locus_tag="JQR05_000715"
     CDS             44788..47202
                     /locus_tag="JQR05_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5322948.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47914..49008)
                     /gene="mnmH"
                     /locus_tag="JQR05_000716"
     CDS             complement(47914..49008)
                     /gene="mnmH"
                     /locus_tag="JQR05_000716"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY5322949.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(49077..50003)
                     /gene="allS"
                     /locus_tag="JQR05_000717"
     CDS             complement(49077..50003)
                     /gene="allS"
                     /locus_tag="JQR05_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY5322950.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            50233..50715
                     /gene="allA"
                     /locus_tag="JQR05_000718"
                     /pseudo
     CDS             50233..50715
                     /gene="allA"
                     /locus_tag="JQR05_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
     gene            50793..51608
                     /gene="allR"
                     /locus_tag="JQR05_000719"
     CDS             50793..51608
                     /gene="allR"
                     /locus_tag="JQR05_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY5322951.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51698..53479
                     /gene="gcl"
                     /locus_tag="JQR05_000720"
     CDS             51698..53479
                     /gene="gcl"
                     /locus_tag="JQR05_000720"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY5322952.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGDIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QALRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            53492..54268
                     /gene="hyi"
                     /locus_tag="JQR05_000721"
     CDS             53492..54268
                     /gene="hyi"
                     /locus_tag="JQR05_000721"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY5322953.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDILGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWIGCEYKPQTTTEAGLRWMDPYR"
     gene            54368..>55236
                     /gene="glxR"
                     /locus_tag="JQR05_000722"
     CDS             54368..>55236
                     /gene="glxR"
                     /locus_tag="JQR05_000722"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY5322954.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANH"
BASE COUNT        13335 a        14198 c        14545 g        13158 t
ORIGIN      
        1 attgcgttgg cggcttgcgc agataaaagc gtggatattc agacgccagc cccggctgca
       61 aatacgtcta tttcagcaac acaacaacct gctatccagc aaccgaatgt ctccggtacc
      121 gtctggatcc gtcagaaagt cgcactgccg cctgatgctg tgctgaccgt gacactttct
      181 gacgcgtcgt tagccgatgc accgtcaaaa gtgctggcgc agaaagcggt gcgtactgaa
      241 ggtaaacagt caccattcag ctttgttctg ccatttaacc cggcagatgt tcagccgaac
      301 gcgcgtattc tgttgagtgc ggcgattaac gtgaatgaca aactggtatt tatcaccgat
      361 accgttcagc cggtgatcaa ccagggcgga actaaagccg acctgacatt ggtgccggta
      421 cagcaaaccg ccgtgccggt tcaggccagc ggtggcgcaa cgactaccgt accttcgact
      481 tcaccaactc aggtgaatcc gtcttcggca gttcccgctc ctacgcaata ttaagcgacg
      541 ttaaccctct ccgcccggag agggttagta gttccagcga taacgctgca agtcgatttg
      601 cccgcttccc gataccatca caccttctgc cagtaatgcc tgtcgctgac gctgtaaatc
      661 cggcccggtt agcgaaattg tgccatggcg attaaccacc cggtgccagg gtaaggtgct
      721 gccttcaggg agacgcttta acacaccgcc cacctggcgc gcggcgcggg gcgatcccgc
      781 cagtttcgcc acatcaccgt aagttgtgac atagccttcg ggaatagcgg cgacgatttg
      841 ccagacgcgt tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc
      901 agcataatct ggaaaaacgc ccgagtgaag tcgcattgcg caagaaacca gcatctggca
      961 cgcaatgggt tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttttcaa
     1021 cgctctcaat gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt
     1081 ccggaaggaa gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc
     1141 acccatttct gcctcccacc gtttcgtcaa aaaatcccag catggctaaa ctttaaccac
     1201 aactgacgtc gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag
     1261 tatgttgatg gttttgtggt tgccgttcct gccgataaaa aggatgccta tcgggaaatg
     1321 gccgctaagg ccgcgccatt gtttaaagag tttggcgcgc ttcgtattgt cgaatgctgg
     1381 gccagcgatg taccggatgg caaagtgacc gattttcgta tggcggtgaa agcggaagag
     1441 aatgaagagg ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgcagct
     1501 aatcaaaaga tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac
     1561 ggcaagcgaa tgatctatgg cggattcgag tcaatcatcg acgaataggt agcgtgacgg
     1621 gctgtgcggc gtgccgtcag cccaaaaatt cacaaatgtt gctcggccca gcgtaagaaa
     1681 tcttctttcg gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg
     1741 cgtagccact cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt
     1801 gtttttgcca tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa
     1861 gatttatcga tcttcagaat gtcgacatcc agatcctgta aataacttaa acttgaatac
     1921 cccgtaccaa aatcatcaag ataaatttca tggcccgcct cccggtagcg agaaattatc
     1981 ggggcgctgg ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg
     2041 ggattaacct gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg
     2101 agcacggtgg attcaagatt gatcgaaata tgctgctgtg gatgctgacg caagcagtcg
     2161 cccatatctt caaagacgct tcttataatc agtagcgtca atggctcaga aaggcccgtt
     2221 tgctgtgcca gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag
     2281 cgcgccagtg cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag
     2341 tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata
     2401 cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt
     2461 aaccaaataa agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg
     2521 atgatcgaaa tattcatctc cgggaagggc aggatatcgt aaatgatccc attattttcg
     2581 atatgctcac cgggtgtttt ttgtagcctg gtaataattc cctgagcaat ttcatcgctg
     2641 ctggttatga caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta
     2701 tagggaatga catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc
     2761 atggcgacca tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa
     2821 ccatctttcg aaattttacc tggctcggga aaggtatcgg gcggactttc atgctccaga
     2881 gacgaacaca ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg
     2941 taagataccc gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt
     3001 tccagctcct gcagcgcatc tttcccttgt gtcgccacct tattggctcg aatagcgacg
     3061 cgggaggaat aggtatccag ctcttcaata aacgatgttt ctacctgctg atgggccagc
     3121 cagatgctta agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg
     3181 accagatgtc gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct
     3241 gattgtggaa aaaggctggc aggagataag gaggtagagc tggtatttca tgaagtgatg
     3301 ggtggaaacg atcattgtca tttgaccagg cgcagagctt tacgcctggt atgtaaccct
     3361 aacttaagat gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag acagttcacc
     3421 tttacggacg atcatttttt gcagttgttc cttatgaaca tccgcgacac gcggagagtt
     3481 caagatggct tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata
     3541 gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa
     3601 aaagaagaaa ccattaagca ggataaataa tacaaccagt agccagacag agcctgacag
     3661 cgtttcggac tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca taccgataaa
     3721 cagccccgga tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc
     3781 gcgggtattc agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg
     3841 ccttcgtggt atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg
     3901 ggaagtaaca gatctaccac aattttgcat aacagttgcg aaaaaccgat tacaattttt
     3961 taattattct ggctggatta ccgcccacga caacgttgtc cgggacacct tttgtgacaa
     4021 ctgcacctga agctaccacg acgttatcac caatggtcac accagggtta atgaccgcgc
     4081 gtccgccaat ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat
     4141 tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca
     4201 tacagttatc accgatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga
     4261 aaaaattatt gccgagaaaa atgttatagc cataatcaca gcgaaacgtt ggctcaatat
     4321 aagcctctgt cacctgaccg aataaatcag cgagaatttg ctggcgtaat gtgtgctctt
     4381 ccgccaggga atgattgtat cggtgaataa gctgacgagc gcgcaggcga tcgcgagata
     4441 acgtctcatc tgccgagcga tacaactcac cagcaatcat cttttctttt tctgtgctca
     4501 ttactgacct ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag
     4561 aacgttctca ttacatctgt gtgataaaga tcacataggg tatgtcttca tggcgaaaaa
     4621 gtataaaatt cttaataaac agccggttat agctccgaaa gcgaattatt tattagcgaa
     4681 taaatttcca tactgaggaa gggatcttgt cgtacagttt attcatggtc aattcggcga
     4741 ggcggtgatc tgcggctgag taaaataccg ccagttcatt atctgataat tcgtatttat
     4801 ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa cgcattaaat
     4861 aatcggtttt tgttaaaggt ttttcggaca tacttctacc catggttgta ataattctaa
     4921 caagataaac tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa tagtcgatta
     4981 cctaacttcc gccatttctg aagagcctgc atattaatac cataactgct gaacaacata
     5041 aaggtgtcat ccagatattc gtcgatctgc tcaatgagct tattgtcttc attatactta
     5101 attttgtaat taagtgcgaa ggtcgcaata tgctcaatca gttcattcaa ctggaggttg
     5161 atcgccgagg ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt tgcaagacag
     5221 tcatgataca gggtttcata gagaaactta agctgtgcga tatcatgtct tttgggtgag
     5281 tattcatcca taacgcgtcc ccttcttagt ggttgaacta acggacacct ttcgggatgg
     5341 aaaaaactta ctgacctgga cttgccctct cgtttgttag cttaactata agccactctt
     5401 tgcaggtttt catcgcatta ttacgaaaaa ttacaattag gaaggaatcg ggacaggact
     5461 cactttttca tactagcact aacccgcagc aggtaaaagc agtttgctcg aactgttcat
     5521 aacatttcag gattatcgcc agatgaaatt ggtgacacta ataccaggat tgctctgaat
     5581 atgacgtaat aaccgaggaa tgaataaaga attaccgcaa ataattaaga atagcctcta
     5641 aatgattatg gattattaaa tctattagcg ctacttaata taaattaact aacaatcagt
     5701 attttttatg aattttatac gtggttaata ctggttttcg tatgagatcc tgagttggtg
     5761 gttcaattac tccttaatgt tcgtaggtta tgcataaaaa aggccgctta cgcggcctta
     5821 gtgattacat gttgtatcaa tgatgatcga cagtatggct gtgctcgata tcttcattct
     5881 tgcggctaaa gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta
     5941 ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac
     6001 cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca
     6061 ggatcggacg taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt
     6121 ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc
     6181 caatggttgt gagcagtcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg
     6241 cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg
     6301 accagctctc gtacagcgcc gccagacaca ggaatacgac aatcaacgaa atcgcgtaca
     6361 gtgaaggtgc ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc
     6421 caacaccggt aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac
     6481 ttttacccgg tgccgcctgg cctaagattt ccatggatgg caggccgttg taacgttcca
     6541 gacgcggcga accgtactcc caacgagaag aggagaacgc cgagaatggc accatctgac
     6601 catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt
     6661 ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac
     6721 cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg
     6781 ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta
     6841 cgctggtcag catatcaggg tgcttcgctg cttctgcaag cagctggtta cgcgcctgag
     6901 tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcag
     6961 tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg
     7021 agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat
     7081 cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc
     7141 cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat
     7201 gcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa
     7261 ccatggtcat aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacga
     7321 acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc cccgtactgc
     7381 gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa
     7441 accagccgaa gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca
     7501 tggtggcaca aagagccgga gtcaggatca acgctaccag taccgacagc gccattgctg
     7561 aaacaatggt aatagagaac tgacgataga tagcaccagt agaaccgcca aagaaggcca
     7621 tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc
     7681 ccatcgactt acgggtagct tcttttggcg gcaaaccttc ttccgccata acacgctcca
     7741 cgttttctac cacaacgata gcgtcatcca ccaacaagcc gatggcgagc accatcccga
     7801 acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca
     7861 ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat
     7921 acataaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag
     7981 agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga
     8041 acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac
     8101 cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt
     8161 cgtagttctc accacccagc tcaatcttcg ccacatcacg cagcagtacg cgggaaccat
     8221 cctgattcac tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag
     8281 caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgacccg
     8341 ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac atcaaccggc gttagctgga
     8401 atttgttcag ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact
     8461 gaacatcacc cacgcccgac gtacggctga tggcatcttt catattcgcc gctacgtagt
     8521 cagagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga
     8581 agctgctgga tgatttctca acgctcaccc cttgctgctg aacttcttgc ggcagcaacg
     8641 gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag
     8701 actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca
     8761 tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg
     8821 ttttcgcatc agcgccgggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag
     8881 gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg
     8941 cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc
     9001 tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta
     9061 tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt
     9121 attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc gccaatagcc
     9181 tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaactac cagtacggtg
     9241 gcatcaccac gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta
     9301 agcccttctt ccagacgtgc gcgcacgaac atacccggca gcagagtgtg atccgggttc
     9361 gggaagatag cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat
     9421 tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct
     9481 ttgccgttct cttgtatcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg
     9541 ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca
     9601 gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt tcgacttacc aatgcgaccg
     9661 ctaatcggag aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct
     9721 ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct
     9781 tgcttactga tgtactgagt accgagcagt ttctgataac gattcaccgt caattgcgcg
     9841 atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgtcgcctga
     9901 taggtcgcag gatcaatctg atagagagag acaccagctt cgatgtcgct accttctttg
     9961 aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca
    10021 ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg
    10081 ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg
    10141 gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc
    10201 atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca
    10261 ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct
    10321 aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc
    10381 gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct
    10441 cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta
    10501 aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac
    10561 tagagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat
    10621 taattcatgt tcttgaatcc acggtgacag aagaacgacg tcgattattg atggagatta
    10681 tattccacaa atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc
    10741 tctgtctgga aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa
    10801 tgttgcctgc ggatttaatg acgcgtcggg cagcaattat tatgcgcggc tatatttccg
    10861 gcctgatgga aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg
    10921 attacgtcgc catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcca
    10981 ctaacgaata accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat
    11041 tctggttcac tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt
    11101 cagaaacctt cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt
    11161 ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg
    11221 gcgtcatcga atggtgatct gccgacaaaa gcggacctgc aggcgcaact tgactcacta
    11281 aataaacaaa aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca
    11341 ttagccaccc tcgataaaat cgatcgcata aaagaagaga cagttcagct acggcaaaaa
    11401 gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagcgat
    11461 gtcgataacg acgaagaaac gcgcaaaatt ctgagtacgc tttcgttgcg ccagctggaa
    11521 actcgcgttg cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat
    11581 aacagccagc tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac
    11641 gcttcgcagc agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact
    11701 gccttacgtc ccagccagaa agtgttaatg caggctcagc aggcgttgct gaatgcggag
    11761 attgaccagc agcgtaaaag cctggaaggg aacaccgtct tgcaggatac cttgcaaaag
    11821 caacgtgatt acgtgacggc gaacagcgcc cgtctggagc accagttgca gctgttgcaa
    11881 gaagcggtaa acagcaagcg cctgacttta actgaaaaaa cggcgcagga agccgtctcc
    11941 ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga aacaggaact ggaaattaac
    12001 cagcagttaa gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa
    12061 aacattaaag tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag
    12121 cagattgccg tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa
    12181 acgctgccct cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc
    12241 gaacagtttg aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac
    12301 aaactggaag aaggtcacac caacgaagtc aacagcgaag ttcacgatgc gttattgcaa
    12361 gtggttgata tgcgtcgcga attgctggat caactcaaca aacagttggg taaccagctg
    12421 atgatggcca ttaacctgca aatcaaccag cagcagttaa tgagcgtatc gaaaaacctg
    12481 aaatccatcc tgactcagca aatcttttgg gtgaacagta accgtccaat ggactgggac
    12541 tggatcaaag cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aatcacggtg
    12601 aactgggaaa aagcctggcc tgccgttttt atcgctttcc tcgccggttt gccgctgctg
    12661 ttgattgccg ggctgatcca ctggcgtctg ggctggctga aagcgtatca acagaaactg
    12721 gcttccgctg tgggttccct gcgtaacgat agccagctca acacaccaaa agcgatcctt
    12781 atcgacctga tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg cctgattctg
    12841 ttgaccatgc agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa actggcgatt
    12901 ttctggctgg tgtttggcct gtgctggaag gtactggaga aaaatggcgt tgccgtgcgt
    12961 cacttcggca tgccggagca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt
    13021 ctcgcgttgc tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg
    13081 atggatgatg tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg
    13141 gtatggccaa tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc
    13201 accattaccg tgctgtcgat tatcccgatt gcattgatgg tgctgactgc cacgggttat
    13261 ttctacacca cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct ggtgatcatc
    13321 tggaacttgc tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc
    13381 tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa
    13441 ccgccggaag aacccaccat tgcactggag caagttaacc agcagacgct gcgtattacc
    13501 atgttgctga tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc
    13561 accgtgttca gctatctcga cagcatcacg ctctggcatt acaacggcac tgaagctggc
    13621 gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg
    13681 gtggcctggg cgttgattcg caacctgcct ggtttactgg aagtgctggt gctctcgcga
    13741 ctgaatatgc gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt
    13801 gccgttggtg cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg
    13861 ctggccgcag cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc
    13921 gtctccggtt tgatcattct gttcgaacgt ccggtgcgta ttggcgatac ggtaaccatt
    13981 ggtagcttct cggggacggt aagtaagatc cgtattcgtg cgacaacgat taccgatttc
    14041 gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg
    14101 tcgttgactg acactactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat
    14161 ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg agcacccaag ggtgatgcac
    14221 gaaccaatgc cggaagtctt ctttacggca tttggtgcca gcacgttgga tcatgagctg
    14281 cgtctgtatg tgcgtgaact gcgtgaccgt agccgtactg tcgatgagct gaaccgtact
    14341 atcgatcagc tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat
    14401 ctgcacaacg agaagggcga tgaggtgacg gaagtaaaac gcgactacaa aggcgacgat
    14461 ccgacaccag cggttgggta ataaccaaag ggggcgacat ttagttgccc cgagattact
    14521 gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg cctacgaaac
    14581 cgcaagaatt caatatattg caggagcggt gtaggcctga taagcgtagc gcatcaggca
    14641 gttttgcgtt tgtccgcaat cttaggggac atttagcggc cccatttatt tctcactttt
    14701 ccgcctcatc atcgtgcgtt aatttctttt cataatcacg ctttacaata tccagcgcgc
    14761 gcagcacggt actggcaggg atctgatttt cctccagcag cacaatcaaa tcgacagcca
    14821 gtttgacatc gtcaggggca ttttcaagtg acatactctc tccattgcta gcgggttaag
    14881 cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag acgcgcttca
    14941 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg
    15001 cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
    15061 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag
    15121 gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc
    15181 acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
    15241 tcgtcgagac aagcctgtag tgttgtcgca cgagtctgaa aaagatgcct gtcgaaacga
    15301 gcacttagcg tggcgaactg tgacaccggg gcacaacgct gacgcagcgt agcgagctgt
    15361 ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
    15421 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
    15481 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
    15541 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
    15601 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
    15661 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
    15721 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
    15781 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
    15841 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    15901 gcacaggcgc aggcggtcaa aggttaaaca acagttactt ttgatacgtt taaaacgcgc
    15961 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    16021 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    16081 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    16141 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
    16201 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    16261 caaactgccg cgtgaaacca tcagtgaaaa ttacgacctg gaatacggca ccgatcagct
    16321 ggaaatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    16381 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    16441 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
    16501 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
    16561 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    16621 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
    16681 tatcaggttt tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    16741 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    16801 ttttccggca cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    16861 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    16921 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
    16981 gatacccgcg aactgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    17041 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
    17101 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
    17161 ccggtgacga ttttgtcacg ctgtctgcaa tttcatctca aggcgctgga tgtcgagcaa
    17221 attcgccatc agcttgagca catcctcaat gaagaacata tcgctcacga gccgcgggcg
    17281 ctgcaattgt tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
    17341 caggcgattg ccagcggtga cggccaggtt tcaacccagg cggtcagtgc gatgctgggt
    17401 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggg caacggcgag
    17461 cgcgtaatgg cactgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
    17521 gtggaaatgc tcggcttgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    17581 ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
    17641 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
    17701 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccgcgt
    17761 atgccgctgc ctgagccaga agtgccacgc cagtcctttg cacccgtcgc gccaacggca
    17821 gtaatgacgc caaccctggt gccgccgcaa tcagcgccgc agcaggcacc gactgtaccg
    17881 ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg cgtgcaggga
    17941 gcaaccaaag caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc ggtgaataac
    18001 gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc aggcgcgtcc ggtgccatcg
    18061 gcgctggaaa aagcgccagc caaaaaagaa gcgtatcgct ggaaggcgac cactccggtg
    18121 atgcagcaaa aagaagtggt cgccacgccg aaggcgctga aaaaagcgct ggaacatgaa
    18181 aaaacgccgg aactggcggc gaagctagcg gcagaagcca ttgagcgcga cccgtgggcg
    18241 gcacaggtga gccaactttc gctaccaaaa ctggtcgaac aggtggcgtt aaatgcctgg
    18301 aaagaggaga gcgacaacgc agtatgtctg catttgcgct cctctcagcg gcatttgaac
    18361 aaccgcggtg cacagcaaaa actggctgaa gcgttgagca tgttaaaagg ttcaacggtt
    18421 gaactgacta tcgttgaaga tgataatccc gcggtgcgta cgccgctgga gtggcgtcag
    18481 gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga taataatatt
    18541 cagaccctgc gtcggttttt cgatgcggag ctggatgaag aaagtatccg ccccatttga
    18601 tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg agagagaaac ctatgtttgg
    18661 taaaggcggt ctgggtaacc tgatgaagca agcccagcag atgcaagaaa aaatgcagaa
    18721 aatgcaggaa gagatcgcgc agctggaagt taccggcgaa tctggcgcag gtctggtaaa
    18781 agtgaacatc aacggtgcac acaactgccg tcgcgtagag atcgacccga gcctgctgga
    18841 agacgacaaa gagatgctgg aagacttggt ggctgcagca ttcaacgacg cagcacgtcg
    18901 tattgaagaa acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc agctgccgcc
    18961 tggctttaag atgccgttct gatgcaaacc agcccgctgt taacacagct tatggaagca
    19021 ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt cacgctgctt
    19081 cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc gatgtcggaa
    19141 atcggccact gcgccgattg ccgcactttc accgaacagg aagtctgtaa catctgttcg
    19201 aatccgcgtc gtcaggaaaa cggtcaaatc tgtgtggtgg agagtccggc ggacatctac
    19261 gccattgagc agacggggca gttttctggt cgttattttg tgttgatggg gcatctgtca
    19321 ccgctggacg gcatcggtcc ggatgatatc gggcttgatc gtctggaaca gcgtctggca
    19381 gaggaaaaaa tcactgaagt gatcctcgcc accaatccca cggttgaagg tgaagctacc
    19441 gctaactaca ttgccgagct ttgcgcgcaa tatgacgtgg aagccagccg aatcgctcat
    19501 ggcgtaccgg ttggcggcga actggaaatg gtcgatggca ccacgctgtc acactccctc
    19561 gccgggcgtc ataagattcg tttttaagca aacgagagca ggagcacctg ctctcgcttg
    19621 aaattattct cccttgtccc catctctccc tcatcctgtt tttaacctta aaatggcatt
    19681 attgaggtag acctacatga aaggacaaga aactcgtggt tttcagtcag aagtgaaaca
    19741 gcttctgcac ctgatgatcc attctctcta ttccaataaa gaaatcttcc tgcgtgagct
    19801 tatctctaac gcctccgatg cggcggacaa gctgcgtttc cgtgcgctat ctaacccgga
    19861 cctgtacgaa ggtgatggcg aactgcgcgt tcgtgtctct ttcgataaag acaagcgcac
    19921 gctgaccatc tccgataacg gcgtggggat gacccgcgac gaagtgattg accatctggg
    19981 gactatcgct aaatccggta ccaaatcatt cctcgaatcc ctgggttctg accaggcgaa
    20041 agacagccag ctgatcggtc agtttggtgt tggtttctac tctgcgttta tcgtggccga
    20101 caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggcg tcttctggga
    20161 atcggctggc gaaggtgaat ataccgttgc cgacatcacc aaagaagatc gtggtactga
    20221 aatcaccctg catctgcgtg aaggcgaaga cgagttcctc gatgactggc gcgtgcgttc
    20281 catcatcagc aaatactccg accatatcgc gctgccggta gagatcgaaa aacgcgaaga
    20341 gaaagatggc gaaaccgtta tctcctgggc aaaaatcaac aaagcgcagg cgctgtggac
    20401 tcgtaacaag tcggaaatca ccgatgaaga gtataaagag ttctacaaac acatcgccca
    20461 cgactttaac gatccgctga cctggagcca caaccgcgtt gaaggtaagc aggagtacac
    20521 cagcctgctg tacatcccgt cccaggctcc gtgggatatg tggaaccgtg atcataaaca
    20581 cggcctgaaa ctgtacgttc aacgcgtgtt tatcatggac gacgctgaac agttcatgcc
    20641 gaactatctg cgcttcgtgc gtggtctgat tgactccagc gatctgccgc tgaacgtttc
    20701 ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc tgaccaagcg
    20761 tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg gaaaaatacc agaccttctg
    20821 gcaacagttt ggcctggtac tgaaagaagg tccggcggaa gatttcgcta accaggaagc
    20881 gatcgccaaa ctgctgcgtt ttgcttctac ttataccgat tcttctgcgc agaccgtgtc
    20941 tctggaagac tacgtttccc gcatgaaaga agggcaggag aaaatctact acatcactgc
    21001 agacagctat gcggcagcga agagcagccc gcacttggaa ctgctgcgta agaaaggcat
    21061 cgaagttctg ctgctttccg accgcatcga tgagtggatg atgaactatc tgactgagtt
    21121 cgacggtaaa ccgttccagt cggtgtctaa agttgacgag tcgctggaaa aactggctga
    21181 cgaagttgat gagagcgcga aagaagcgga gaaagcactg actccgttca tcgaccgcgt
    21241 gaaagctctg ctcggcgagc gcgtgaaaga tgtccgtctg actcaccgtc tgaccgatac
    21301 gccagcgatc gtttcgaccg acgcggacga aatgagcact cagatggcga aactgttcgc
    21361 tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc gaactgaacc cggatcacgt
    21421 actggtgaaa cgtgcggcag atactgaaga tgaagccaag ttcagcgagt gggtagaact
    21481 gctgctggat caggcgctgc tggcagaacg cggcacgctg gaagatccga acctgtttat
    21541 tcgtcgtatg aaccagctgc tggtttcctg atgtcatgcc ggatgacctt tgtgtcatcc
    21601 ggcatttttc ttttcatcat ctgcactttc cgcaaattat ctcgccatta accgtttcag
    21661 cctcaggtgc ctttcttgag gcaatcgcct gttggtggta tcgtttatcg ctttttcaaa
    21721 aaaatcgaca cattttaagg ggattttcgc aatgcgtatc attctgcttg gcgctccggg
    21781 cgcggggaaa gggactcagg ctcagttcat catggagaaa tatggtattc cgcaaatctc
    21841 cactggcgat atgctgcgtg ctgcggtcaa atctggctcc gagctgggta aacaagcaaa
    21901 agacattatg gatgctggca aactggtcac cgacgaactg gtgatcgcgc tggttaaaga
    21961 gcgcattgct caggaagact gccgtaatgg tttcctgttg gacggcttcc cgcgtaccat
    22021 tccgcaggca gacgcgatga aagaagcggg catcaatgtt gattacgttc tggaattcga
    22081 cgtaccggac gaactgattg ttgatcgtat cgtaggccgc cgcgttcatg cgccgtctgg
    22141 tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa ggcaaagacg acgttaccgg
    22201 tgaagaactg actacccgta aagacgatca ggaagaaacc gtgcgtaaac gtctggttga
    22261 ataccatcag atgactgcac cgctgatcgg ctactactcc aaagaagcgg aagcgggtaa
    22321 caccaaatac gcgaaagttg acggtaccaa gccggttgct gaagttcgcg ctgatctgga
    22381 aaaaatcctc ggctaatcaa gccgcagcag atctgctttc gggcaggtct gctcatccac
    22441 ctcaggcaat acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc
    22501 aacaagttga atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca
    22561 aacctgggta cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt
    22621 ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc
    22681 gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt acgcctctgt ctggatggaa
    22741 gacggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta
    22801 ccggatacgc cagtggcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc
    22861 gatgaactcc tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc
    22921 tcctgttcta cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt
    22981 agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca
    23041 ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc
    23101 tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc
    23161 cgcacaacga ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg
    23221 acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaagcgctg
    23281 aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg
    23341 gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcg
    23401 ggcgggaaaa aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg
    23461 atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg
    23521 cgaagagcct cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc atgcagcgta
    23581 cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga
    23641 cggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
    23701 cgagtgagat cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt
    23761 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc
    23821 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc
    23881 gcaattttac cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc
    23941 atggcacctg cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc
    24001 gcctgctggt ggaaataaca acaagcagcc acaatttcct ctatcgcttg tggaaaacgc
    24061 gcttcaggtg aaagggtgta atcaatacca atcaccgtac attggctgta gcttgccagc
    24121 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
    24181 taaaatagcg tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca
    24241 tattttgttg gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat
    24301 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac
    24361 ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga
    24421 acaggtagtt tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg
    24481 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
    24541 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
    24601 aaatttcccg gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg
    24661 cttcagcaat tccagccaga aaacgaaacc agcgccgctt gggtcgtagg tattgaccag
    24721 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
    24781 gccgggcatt cactggtgat tgaggatgac gtagccgaag cgctttatca ggagctaaaa
    24841 cagaaaaatc tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac
    24901 tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa
    24961 attggcagtt atgcctatcg ttacctgtgt aacacttcca gccgtaccga tcttaactat
    25021 ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa
    25081 cgtacctttg ctatcagtcc aggccacatg aaccagctgc gggctgaaag cattccggaa
    25141 gatgtgattg ccggagcctc ggcactggtt ctcacctcat atctggtgcg ttgcaagccg
    25201 ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta
    25261 ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag
    25321 caattcctca aagaccacgt ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg
    25381 accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg
    25441 ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa
    25501 cgtaaaaccc agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt
    25561 agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtctattc gcacattgcg
    25621 ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg
    25681 gcagcgttgc tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc
    25741 agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct
    25801 aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg
    25861 gagcatgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag
    25921 gccagataag gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg
    25981 gcgcgtctta tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc
    26041 cgtcaccact tcacccgccg gtggcgtttc cagcagttcc agcatagtac gggcaatttc
    26101 acgttcaccc atcactacct gattcgcgcc acgttcggtg atatacgtca cttcatcgtc
    26161 ataatgggcg cgggcgataa tctcaatatc cgggtttttc gcgcgggcag acgccacaat
    26221 ctcacccgct tcataaccgt tggggatcgt caggatcagc cattttgcac attccagatg
    26281 tgccagttgc ataatttctt cgttcgctgc attgcccaat acagcgcgga ccccacgctc
    26341 tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc
    26401 gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccaata gcgcatggtt
    26461 gcaaatatcc actgggatct gtttctcttc ttcgattgcc tcttccagcg tctgctcttc
    26521 cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat
    26581 aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa
    26641 tgccattccc agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc
    26701 gatggttaat gccgtacgtt gggagtgacc aaacagacgg acaaggaaca gggccgcgac
    26761 cgatttacca aacagaataa ttgccagcgt cgccagcact gccagcggtt gctgaatcag
    26821 aattaacgga tcaaacaaca tcccgactga gacaaaaaac agcaccgcaa acgcgtcgcg
    26881 caatggcaac gtatcgtggg cggcacggtg actcaattca gactcgttca gtaccatccc
    26941 ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat
    27001 ccctaacgcc agcgccagca ctgacagggt aaacagctcg cgagaaccgg ttgccgcgct
    27061 gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc
    27121 gatcaccttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc
    27181 ctgttccatc attcctgcca ctgcgggcag caacaccagc gtcagaacca tcaccaggtc
    27241 ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtctatta attgccgttc
    27301 ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataagc cgaacacgat
    27361 accggtcatt aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac
    27421 ggctatctgg gcgatcgcgc cgggaatggc gatggacttt accgccatca aatccttgag
    27481 cgaaaaatgc aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg
    27541 cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa
    27601 cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc
    27661 gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc
    27721 ttttcctggt ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt
    27781 atgacaattt cattgatgat gtttatgaat aattgttgaa ttttgcagaa aaatggaatt
    27841 agctgcaaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg
    27901 gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg
    27961 ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt
    28021 atagactaac tcgatgctgg tgtgatcggc gataagcccc agaactgccg ctcccagacc
    28081 tcccatgccg aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag
    28141 tagctcctga gcgtagacca gaatggcaga gaatgccgaa gcgaggataa atccaataat
    28201 caccgttaaa acccccatcc agtgcaggcg ggcgtagggt aaaatcagcg taaacggcgc
    28261 aacgccgagg atagagcccc aaatcacata tttccgtcca attttatccc ctacaggccc
    28321 gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgggcatt
    28381 ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc
    28441 catatagaaa tatttcgaga aaatgaggat taacagaatg ctgactgcca gtaccacttt
    28501 gttgcgcggc agtggattga ttatcgtcgc tttgggtttt cctttattca ttcggtgctg
    28561 tgccgagtac caacggctaa tttgcgccaa caccacgatc gccagcagtg ccgcaagcac
    28621 aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc
    28681 cagggaactg ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc
    28741 gccggaagcc atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc
    28801 gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag
    28861 accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt
    28921 ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat
    28981 gccaatctgc ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat
    29041 cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa
    29101 cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg tttgttcact
    29161 cattgccata ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga
    29221 ttattacccg actaacatac ctgtatgcgt cgtctgaagg aagtctcaac gccgaataca
    29281 gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc
    29341 gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca
    29401 gcggggcgtg gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc
    29461 gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca
    29521 tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg
    29581 tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat
    29641 taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa
    29701 tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt
    29761 attacgtcag caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa
    29821 aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat
    29881 tgccgttatt gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac
    29941 tgatatcgaa tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca
    30001 gacagaaaag ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga
    30061 gtacggctct aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct
    30121 ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa
    30181 acaggtcgat tacgtgccgg gtacgccatg caaaccagat caacaaaacg gcatctggat
    30241 tgtgcaggcg catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg
    30301 cgaaatgaaa atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg
    30361 ggaagacggg aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat
    30421 gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg
    30481 cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat
    30541 ggggcggttg attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag
    30601 cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa
    30661 agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga
    30721 ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa
    30781 cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag gcgaaccggt
    30841 cgatccggcg aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg
    30901 atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt
    30961 gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg
    31021 tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat
    31081 cgcggcgggc gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca
    31141 gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg
    31201 gggcgtcgat aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca
    31261 ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca
    31321 gcgcttttgc tactttttta agatccagtt gacctgcgac cggcgtaacg gccacggcaa
    31381 ggtgtttcat atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca
    31441 aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg
    31501 tatggatttg aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa
    31561 ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa
    31621 gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac
    31681 cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata
    31741 gcgatattgg ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg
    31801 tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca
    31861 ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag
    31921 cgttatatca ttattttgcg gcggtgcatt cagccaccag ctcatcattt gttgcagcaa
    31981 ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg
    32041 gagctggagc aacatggcaa tctggttttc agccccttcc agttcaatca cgggtttatg
    32101 ttgttgcttc gccgcctgca atagctggta atcgataccg tattctgccc gcagccccag
    32161 tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa
    32221 gagtgaggga gaaatgccca tctcctggct aatgtgctgc aggttttgga gttgctcctc
    32281 gctaatgcgc tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg tatcgctggt
    32341 ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt
    32401 gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg
    32461 attgccgggg agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc
    32521 ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc
    32581 tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga
    32641 ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg
    32701 caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg
    32761 atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatcgc gtcagtgcac
    32821 tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg
    32881 ataccacagt cgattttagg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa
    32941 acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac
    33001 gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc
    33061 cggataaggc gttcacgccg catccggcat ccgagcacca ttgcctgatg cgacgcttac
    33121 gcgtcttatc aggcctacaa acctgtgcct gaaccgtagg ccggataagg cgtttacgcc
    33181 gcatccggca gtcatgcgtc gatgccaaat gcgccgccct aaagcagcgc atccgcaatg
    33241 acgtacttat tccttcggtt taaaccgcag caaccggttg gcgttactca ctacggtaat
    33301 cgacgagagc gccattgccg ctccggcaac taccgggtta agcagtgttc cagtaaacgg
    33361 ccacaaaata ccggcggcga ccggaatacc gatgctgttg tagataaagg caccgagcag
    33421 gttctgcttc atgttgcgca gcgttgcgcg ggaaatcgcg agcgcatccg caacgcccat
    33481 caggctatgg cgcatcaggg taatcgccgc ggtttcaatg gcaacatcac tgccgccacc
    33541 catcgcaatg ccgacatccg cctgggccag cgctggcgcg tcgttaatgc cgtcgcccac
    33601 cattgccacc tgacgtcctt cactttgcag acgtttgatc gcttcggctt taccgtccgg
    33661 cagcactccg gcaatcactt catcaattcc agcttctttg gcgatcgcat tggcggtggt
    33721 tgggttatcc ccggtcaaca tcaccagacg atatcccgct ttatgcaggc gttgcagcgc
    33781 cgccacgcta tcactacgca acggatcgcg tacagccagc agggctactg ctttcccgtc
    33841 aatcgccagc agcacaggcg ttgctccttg cgatgcctga gctgaaatat cggcttcaat
    33901 cgctttagta tcgacttgct gatcatttaa caacgcctga ttacccagca ataacgcatg
    33961 accttctgct tcaccgctca cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg
    34021 tagctgcata tcgcctgctt tatcaaggat cgctcgtgcc aatggatggc tggaaccttg
    34081 ctccagcgcc gccgccagac gcagcgcctg cgcttcgtca acatcagcaa atgttttcac
    34141 tgcgacaacc tgcggcttcc cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc
    34201 gagtgtactg gcgcgttgca gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc
    34261 ccgcccgacg ccggaaataa tcgacatcgg cgtcgccagt cccagcgcac acgggcaggc
    34321 gataatcagc accgtagtgg caatcaccag ggtatagaca atctgcggtg ccggaccaaa
    34381 gaaataccag attgccgcac tgacaagcgc aataaccact actaccggca caaatacggc
    34441 tgagatttta tccgccagct gaccgatttc tggcttgctg ctctgggcct ggcgcaccat
    34501 gcgaatgatc cttgatagcg tggtatggct gccaaccgca ctggcacgaa acagcacact
    34561 gccgtcctgt accactgtcc cggcatggac gctatcgcct tcgccttttt gctgcgggat
    34621 tggttcgccc gtcagcatcg cttcatccag ccatgcttcg ccccgggtaa tctcgccatc
    34681 taccggcacg cgatcgccgg tcgtcaggcg cagcaacata cctggctgca cttctgccag
    34741 aggcacgctt ttttcacctt cgtcagtaac caggcgcgcc gtcggtgggg ttaaatcgag
    34801 taatttctcc agtgccttag aagaacgctg acgtgcgcgt gcttccagca tatggccgag
    34861 attgatcaga ccgataatca tcgcactggc ttcatagtaa agatgtcgcg cttccatcgg
    34921 gaaccactgc ggccacaggt tgacgctcat cgaatagagc cacgccacgc ccgttcccag
    34981 cgccaccagc gtatccatcg tcgctgcacc gttcagcagg cttttccatg cactgcggta
    35041 aaaatggccg ccggcgaaaa ccatcaccgc cagggttatc aggccgataa ccaaccacag
    35101 gctgcggttg tcggcggtga ccatcatgtt atcgccgatc atcccccaga ccatcaccgg
    35161 gatacccacc gccagtgcga caattgcctg ccagcggaag cgcttcatcg tagcgacggc
    35221 ggtttcttgc tggcgttcgc ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc
    35281 cgctttttcc accgcctgca ctaaatcttg tggggaggca ctgcccatca ccagcgcagt
    35341 acgctccgcc aggtttaccc gtgcctgagt gacgcccggt acgctttgca gcgcattttg
    35401 tacgcgggtg acacagctgg cgcagctcat gccgctcagc agcaactgct ggctgtcatc
    35461 gtcatcggcg gtcgctgccg gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat
    35521 tgatgactcc gccagcggtt tagcctttgg gtggcttaca gatgcgtcat aacccgcttg
    35581 tttgatggtt tcgatcagct gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat
    35641 agacacatcc gcttgttcca catccgggcg ctgttcaaga ctttctttca cgcgtttaac
    35701 gcagtgaccg caggacaggc cgtccaggtt caggtcgata gtttgtgaca taaaacactc
    35761 ctttaagaca gttttgactg gctgtgataa aggttaaacc ttccagcaag gggaaggtca
    35821 agaaattaat aaaccaggcg ggtaaaagtc cgtaaagatt aaaaaatcgg ctcgatttgc
    35881 atcaggatta gacatttatt tctttgtttt cctgtagtta agttgcgggt gctaagttaa
    35941 atcaggatgc ctgaaaatcg gcaccggggt gaggaattac ctcccgcatc tataaaaagg
    36001 agttaacaaa agatgttaga tgcaaacaaa ttacagcagg cagtggatca ggcttacacc
    36061 caatttcact cacttaacgg cggacaaaat gccgattaca ttccctttct ggcgaatgta
    36121 ccaggtcaac tggcggcagt ggctatcgtg acctgcgatg gcaacgtcta tagtgcgggt
    36181 gacagtgatt accgctttgc actggaatcc atctctaaag tctgtacgtt agcccttgcg
    36241 ttagaagatg tcggcccgca ggcggtacag gacaaaattg gcgctgaccc gaccggattg
    36301 ccctttaact cagttatcgc cttagagttg catggcggca aaccgctttc gccactggta
    36361 aatgctggcg ctattgccac caccagcctg attaacgctg aaaacgccga acaacgctgg
    36421 cagcgaattt tacatatcca acagcaactg gctggtgagc aggtagcgct ctctgacgaa
    36481 gtcaaccagt cggaacaaac aaccaacttc cataaccggg ccattgcctg gctgctgtac
    36541 tccgccggat atctctattg tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc
    36601 tctacgctca tcaatactgt tgaactggca acgcttggcg cgacgctggc ggcgggtggt
    36661 gtgaatccgt tgacgcataa acgcgttctt caggcagaca acgtgccgta cattctggcc
    36721 gaaatgatga tggaagggct gtatggtcgc tccggtgact gggcgtatcg cgttggttta
    36781 ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg tccctggcgt gatgggaatt
    36841 gccgcgttct caccaccgct ggacgaagaa ggcaatagtg ttcgcggtca aaaaatggtg
    36901 gcatcggtcg ctaagcaact cggctataac gtgtttaagg gctgatcatg atgaacacgg
    36961 aaggtaataa cggtaacaaa cccctcggtc tatggaacgt cgtttccatc ggtattgggg
    37021 caatggtggg ggcggggatc ttcgcgctgc tggggcaggc tgcgttgcta atggaagcct
    37081 cgacctgggg cgcctttgct tttggcggta ttgtggcgat gttttccggt tatgcctatg
    37141 cgcgtctggg ggcgagctat ccctgcaatg gcggtattat cgactttttt cgtcgcggat
    37201 taggcaacgg cgtcttttcg ctagcgctct cgttgctata cctgttgacg ctggcggtga
    37261 gcatcgccat ggttgcccgt gcttttggcg cttatgccgt gcagtttttg catgaaggca
    37321 gccaggagga gcaccttatt ttgctctacg cgttggggat cattgcggtg atgacgcttt
    37381 tcaactcctt aagcaaccat gcggtagggc ggctggaagt gatcctcgtc ggcattaaaa
    37441 tgatgatcct gttattgctg attattgccg gtgtctggtc gctgcaaccg gcgcatattt
    37501 ccgtctctgc gccccccagc tccggtgcgt tcttctcctg tattgggata accttccttg
    37561 cctatgcggg ctttggcatg atggcgaacg cggcggataa agtgaaagat ccgcaggtca
    37621 ttatgccacg ggcgtttctg gtggcgattg gcgttaccac gttgctttat atctcgctgg
    37681 cgctggtttt gcttagcgat gtatcggcat tagagttaga aaaatatgct gataccgccg
    37741 tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta tgtgatcgtc gtcatcggcg
    37801 ctttactggc gacggcttca ggcattaacg cgaacctgtt cgccgtgttt aacatcatgg
    37861 acaacatggg cagcgaacgc gaactgccga agctaatgaa taaatccctg tggcggcaga
    37921 gtacctgggg caacatcatc gtcgtggtgt tgataatgct gatgacggcg gcgctaaatt
    37981 tagactcact cgccagcgtt gccagcgcca cctttttgat ctgctatctg gcggtgtttg
    38041 tggtggcgat ccgcctgcgt catgatattc acgcctcgtt gccgattctt atcgttggta
    38101 cgttggtgat gttgttggtg atcgttggct ttatctacag cctgtggtcc cagggcagcc
    38161 gtgcgttgat atggattatt ggtgcaatct tactcagcct tattgtggca atggtcatga
    38221 agcgtaataa aaccgtataa cacctctctg tgcgcagtac ttcctgtatt attgtggtgg
    38281 cggtcgatat tcgcactggc aaaaaaacgt gcttgaatat ctgttgaaac cctttaacaa
    38341 agcacaggag gcgttgcgcg aacgatgaac atcagcgatg tagcaaaaat taccggcctg
    38401 accagcaaag ccattcgctt ctatgaagag aaggggctgg tgacgccgcc gatgcgcagc
    38461 gaaaacggtt atcgcaccta cacgcagcag catctcaacg aactgacctt actgcgccag
    38521 gcacggcagg tgggctttaa cctggaagag agcggcgagc tggtgaatct gtttaacgac
    38581 ccgcagcggc acagcgccga cgtcaaacgg cgcacgctgg agaaggtggc ggagatcgaa
    38641 cgacacattg aggagctgca atccatgcgc gaccagctgc tggcactggc gaatgcctgc
    38701 cctggcgatg acagcgccga ctgcccgatt atcgaaaatc tctccggctg ctgtcatcat
    38761 cgggcagggt gattaagacg agacggctcg gatatgtagg gttatccctt ctatcgcaat
    38821 gacttcaaca tgcgtacctg caccgagatc ctcgctggcg ctgacaggcc atgaactgtc
    38881 accgacgcgc atatgaccgc gcccgttgac cagcggagat tccagcacaa aacgtcggcc
    38941 aatcagctgc tgcccgcgct ggtttaaatg actatcgctg tgcttttgtt cgcgcacccg
    39001 ccgcgacaac catttccacc acagccaggc ggcgagcagc gtcaggatgg caaacattac
    39061 cccttgccac tcccaaccca gcggcaccag ccagaccacc aggccggtaa tcaccgctgc
    39121 cacgccgctc cacaacaaat aaccatttcc gcccagcatc tcggctgcca gcagcaaccc
    39181 gccgagactg agccagaaaa tatgtggatg aacgaccatt aactccatca tggctgagtc
    39241 cgtttgttgg cgctgtcttt caccagctcg gcaatcccgg caatcgaccc catcaggctg
    39301 ctggcctcta atggcatcat cactactttg ctgttactgg aggaaccgat ctgctgtaac
    39361 gcttcggtgt atttttgcgc tacgaagtag ttcaccgcct gaatatcacc ggaggcgata
    39421 gcctcagaca ccattttggt ggcgcgggct tctgcttcgg cggaacgttc acgcgcttca
    39481 gcctgtaaaa acgccgactg acgttcgcct tccgctttca ggatttgcga ctgtttttca
    39541 ccttcggctt tgaggatttc cgcctgacgg atcccttccg cttcaagaat gtaagcgcgt
    39601 ttggtacgtt ccgctttcat ctgcgcgttc attgaagaga taagctctgc cggtgggcgc
    39661 acgtcgcgaa tttcaatacg ggtgacttta atcccccacg ggttggtggc ttcatcgaca
    39721 atacgcagca ggcgtgagtt gatgctgtcg cgctgagaga gcatttcgtc aagttccatt
    39781 gaacccaaca cggtacggat gttagtcatg gtcaggttga tgatcgccag ctccagattg
    39841 ctgacttcat aggcagcgcg tggcgcgtca atcacctgaa taaaacagac tgcgtcgatg
    39901 gtaacgttgg cgttatcttt cgagataact tcctgggaag ggatatcgag cacttgctcc
    39961 atcatattga tcttgcgacc aatgcgatcc ataaacggca ccaccagact gagccccggc
    40021 tgtaacgttt tggtatagcg accaaaacgt tctaccgtcc attgatagcc ctgcggcacg
    40081 attttgacgc ccgcgccgac aatgaccagc gcgacaaaaa tgagaatcgg gataaagata
    40141 agcatcggaa aaacctcctg ttgtaccgtc cataatcagc aaaattgctg cttgattaaa
    40201 caaattatac ctgattactg aaagagagtt cccccttatt cctgcgaagg ataaactgtt
    40261 tttagtaaaa atcagaaaaa gggaacagcg atgcaggaaa atagtccttt gcttcagcta
    40321 caaaacgtag gatatctggc gggtgatacg aagattctta ataacatcaa tttttcgctg
    40381 cgtgctggcg aatttaagtt aattaccggt ccttctggtt gtggcaaaag tacgctgcta
    40441 aaaatagttg cttcattgat cagcccaacc agcggaacgt tactgtttga aggtgaggat
    40501 gtcagcacac taaaaccaga aatctaccgc cagcaagtct cttactgcgc ccagacgccg
    40561 acgctgtttg gcgatacggt atacgataat ctgatctttc cctggcagat ccgtgaccag
    40621 cagcctgacc cggccatttt tctcgatttt ctcgaacgct tcgctttgcc ggacagcatt
    40681 ttgacgaaga atatcgccga gctatctggt ggtgaaaaac aacgcatctc attgattcgt
    40741 aacctgcaat ttatgccgaa ggttttattg ctggatgaaa taaccagtgc gctggatgaa
    40801 agtaataaac ataacgtcaa tgagatgatc catcgttatg tgcgcgagca aaatattgcc
    40861 gtgctgtggg tgacacacga taaagacgaa attaatcatg cggataaagt gattacactg
    40921 caaccgcatg ccggagaaat gcaggaagca cgctatgaac tcgcataata ttactaacga
    40981 atcattagca ctggcattaa tgctggtggt ggtggcaatc ttaattagcc ataaagaaaa
    41041 actggcgctg gagaaagata ttctctggag cgtcgggcga gcgataattc agctgattat
    41101 tgtcggctat gtgctgaagt atattttcag cgtggatgat gccagcctga cattattgat
    41161 ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa aaacgcagta aatatattgc
    41221 taaagctttt atctcatcgt ttattgccat tacggtcggg gcgggaatta ccctggcggt
    41281 gctgattctc tcagggtcga ttgaatttat cccgatgcag gtgatccctg tcgccgggat
    41341 gattgccggt aacgccatgg tagcggtggg gttgtgttac aacaatttag ggcaacgggt
    41401 cattagcgaa cagcaacaga ttcaggagaa actgagtctt ggcgcgacgc cgaagcaggc
    41461 ttcagcgata ttgattcgcg acagtattcg cgcggcttta attccgacgg tcgattcagc
    41521 aaaaacggtt ggcttagtga gtttaccagg aatgatgtcc gggctgatat ttgccgggat
    41581 tgatccggtg aaggcgatta aatatcagat tatggtgacc tttatgctgc tctcaaccgc
    41641 cagcttgtcg accattattg cctgctattt aacctatcgt aagttttata attcgcgcca
    41701 ccagttggtg gtgactcaat tgaagaagaa atgatgcgat gccggatgcg cagcatcgca
    41761 tccggcgttg tggttcatgt gccgaacaac ggaatatcaa tacaacaatg catacagctg
    41821 gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc gcagcgagga tctcctggaa
    41881 cgttttacgc gtctgaccgt ctgcggcggt gagatcttta cgcagatgcc cgaacagcaa
    41941 ctccagcgcc tcttcattgc gcccaacctg atgcagttgc agcgccagtt gcgtcgccag
    42001 tgcggcatct tctggattct ccgccacctg ctgttgcaac tgttgaattt ccggcttatc
    42061 agccgcctgc ttcagcagtt cgatttgcgc taccagcccc tggtagcggg tgtcctgatc
    42121 ctgcaacgga atggttttca gcaccgcttc cgcatcttca gaacggttca gcgcaatcag
    42181 cgtttctgcc agcagcaggc cgatctcccc gttctgattc gacaactgcc aggcgtcttt
    42241 cagcaatggc agggcatcgg tgtaattgcc ttcctgcatc agttgcatcg cctgctgcgc
    42301 tttcagctct tcttcgcgcg gcagcacttt atccagcagg gcgcggatcg cctcttccgg
    42361 ttgcggcccc tggaagccat ctaccggttg cccgttctgg aacagataca cggtcggaat
    42421 cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg tcgcagtcca gcttcgccag
    42481 aataaattgc ccgttgtact gcgccgcgag gctttccagg atcggggtta actgcaaaca
    42541 gtgctggcta cgttcagacc aaaaatagaa cagcaccgga gtggtcatcg actgttcaag
    42601 aacctgttgc aggttagatt cgttaatgtt gacaatattt tctacggaca tggagtcgct
    42661 ctctgttgtc gatattttct ttgacatggg ggcttaagcg cgcgcttcaa ctcacccctg
    42721 caatattttg tccatcacgc gcccaggcag caggcgctcg agcaccatta ctgcccaggt
    42781 caccagcgtt accggatagc gcatcttcgg cttctcgcta ataaaagcat ggcgcacttt
    42841 gtcgaccacc gcttccggtc ccaacgtaaa gcgggcggcg atgccgggat tttcgactgg
    42901 tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga gtacgaatgg gaccgggttc
    42961 gatcaggctg actttaattc cgctgtggcg tagctccatg cgcagcgcgt ctgaccacgc
    43021 ctccagcgca tatttactgg ccgcgtaagc gccacgaccc ggcgtggaga ttaatcccat
    43081 caccgatgat gtcatcacaa tacgcccttc accgtgcggt aacatcgcgg gtaacaggcg
    43141 catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac tgctgttcca tctgcgcacg
    43201 gctgatggtg gaaagggggc catacatgcc gaatccggca ttgttaaaga tcccatacag
    43261 acaattatcg gtcagggcga tcacctcgtc tgctgcgcga ttaacacttt ctggtgaatc
    43321 cagatcgatc aacacgccgg taaatcccat gttgttcata cgctcaacat cattcggttt
    43381 tcggcaacct gccagcacat gaaaaccctg gcgttttaat tcgagcgcgc tttccaggcc
    43441 aattccactg gaacatccgg taattaagac cgatttttgc ataactttac ctgtcaggat
    43501 ctccgttgct ttatgagtca tgatttacta aaggctgcaa ctgcttcgcc atccagtcgg
    43561 caataaacgg ctgggcgtcg cggttgggat gaataccgtc atcctgcatc cattgtggct
    43621 tgaggtagac ctcttccata aaaaagggca gcagcggaac atcaaactct ttggcgagtt
    43681 tggggtaaat ggcgctaaag gcttcattat aacggcgacc atagtttgca ggcagacgta
    43741 tttgcattaa caatggttca gcgttggcgg ctttgacatc ctgcaaaatc tggcgcagcg
    43801 tttgctcggt ttgctgtggc tgaaaaccac gcaaaccatc attgccgccc agttcaacca
    43861 gcacccaacg cggctgatgc tgtttgagca gagccggaag gcgcgccagc ccttgttgcg
    43921 aggtgtcgcc gctgatgctg gcattaacta ccgacgtttt actctgccac ttatcattca
    43981 acaaggcagg ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg ctatcaccca
    44041 gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg
    44101 gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg
    44161 ggagcatgaa ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat
    44221 cgcactggtg ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct
    44281 tgatgacggc agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga
    44341 agaagcgcgg gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt
    44401 aattcctacc cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc gcggtgagag
    44461 tagcgcggaa agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc tgggtaaacg
    44521 tctggatcat cttccggcgc agctttccgg cggtgaacag caacgagtgg cgctggcacg
    44581 agcctttaat ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca accttgaccg
    44641 ccagacgggc gataaaattg ccgacctgct gttttccctc aaccgtgaac atggcaccac
    44701 gttgattatg gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct
    44761 ggtgaacggg cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgt
    44821 tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg
    44881 ctgggcaata tcagcgatcg catggagaag ggcttaagcc agcaaagccg tgagtttatg
    44941 gcgggcgatc gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct ggaggaagcg
    45001 caaaagcgcg gcctgaaagt cggcaagcag ctgactttcg ccacaatgac ctttgcaggc
    45061 gacacaccgc agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcgat
    45121 ctgcaaacta atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc
    45181 ctgatggcac tgcttaacct gaaaacgggc gacaccattg acgtgggcga tgccaccttg
    45241 cggattgccg gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct
    45301 ccgcgtctga tgatgaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt
    45361 cgggtcacct ggcgttataa attcggcggc aacgagaacc agctcgacgg ctatgagaaa
    45421 tggttgttac ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc
    45481 gcgctggggc gatcgatgga acgctcgcaa cagttcctgc tgctttcggc gcttctgacc
    45541 ttgctgctgg cagtggcagc ggtggcggta gcgatgaatc attactgtcg cagtcgctac
    45601 gatctggtgg cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc
    45661 gtcggtcagt ggttgatggt gctgacgctt tcagccgtta ccggtggggc cataggcctg
    45721 ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca
    45781 gccagcctct ggccgtggct gtgggcgctt ggcaccatga cggtcatctc gctgctggtg
    45841 gggctacgac cataccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat
    45901 gtggtcgcga acgtctggcc gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg
    45961 ctgctgctcg ccggattaat gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg
    46021 gtagtactgg ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt acttcgccgc
    46081 atgacgctga aatcgctgcc tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg
    46141 tcaacgttaa gccagctttc ggcattttcg ctctccttta tgctgctggc actgctgctg
    46201 gtgctgcgtg gcgatctgct cgaccgctgg cagcagcagc taccgccaga aagcccgaac
    46261 tactttttaa ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa
    46321 catcatatag tcccggaatc attttatccg gtagtgcggg cgcggctgac ggcgattaac
    46381 gataagccga cagaaggtaa tgaagatgag gcgcttaatc gcgaactcaa tcttacctgg
    46441 caaaatacgc ggcccgatca taatccgatt gtcgccggta actggccgcc aaaagccgat
    46501 gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg
    46561 acttttatgg gcgataccca ggagttccgc gctaaagtga ccagcctgcg caaagtggac
    46621 tgggaaagtc tgcggcctaa tttctatttt attttccctg aaggggcatt agacgggcaa
    46681 ccgcagagct ggcttaccag tttccgctgg gagaatggca acggcatgtt gacgcaactc
    46741 aaccgccagt tcccgaccat tagcctgtta gacattggcg cgattttaaa acaggtcggt
    46801 caggtgctgg agcaggtaag tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc
    46861 ggtatgttgc tgttgctggc acaggtgcag gtgggtatgc gccagcgtca tcaggagctg
    46921 gtggtatggc gcacactcgg tgcggggaaa aaactgctgc gcacgacatt gtggtgtgag
    46981 ttcgccatgc ttgggtttgt ttccggcctg gtggccgcaa ttggtgcgga aacggcactg
    47041 gcagtgttgc aggcgaaagt gtttgatttc ccgtgggagc cagactggcg attgtggatt
    47101 gttctgccgt gcagcggtgc gctgctgctg tcgcttttcg gcggctggct gggtgcgcga
    47161 ctggttaagg gtaaggcgct gttcaggcag tttgtggggt gatgacaggt gttagattgc
    47221 ctgatgcgct acgcttatca ggcctacgaa gagcgtgcaa tatattgatt ttgcaagatt
    47281 gtgtaggccg gataaggcgt ttacgccgca tctggcatga acagtgtgca ttttggcaat
    47341 aaatatgaaa ccaactgtca tgtcggtttt tttaaaggcc gaaggaggta aaccttcggc
    47401 cttagctacg tttcctcagt tattcaataa actgacggat cagttccagc agcgcgatta
    47461 gcgccttaag aacgatgaga actttatcga gaaaagtttt catccgttgc tcccttaagg
    47521 gagaaagaca atcacagctt acccttacgt tgcctgtaac tatgattttc ttcatgtgct
    47581 agatgaggac tgcctctctc caaattagag caccagaatc agcaccacct gtatcctgac
    47641 caggacttaa aagtactttt ccgcactgcg gtaatccctt gaggcagtat cactactagc
    47701 gtcgggattg tatcgccaac atagctgttt gaatatgtag gcatcctgac ttataattca
    47761 cttgctagat gaggactgtt taatttctgc ccggaaccac caagtcttgg gcggaaaaaa
    47821 taaaaaaacc gactgtcatg tcggtttttt atatctgtat tctggtgcaa ccattttgtt
    47881 ctcacgacaa tcgccgtttc agaataccgc ccatcactgc gccttaaccc ataccgccac
    47941 ttccgcccac tcaccgcgaa agacaacttt ttccgctttt ttctcaagct gatagcgata
    48001 cattgggtcg taatattctt taagtaacgg caccagccag gccagatgac cgtcggtgct
    48061 gccggtggtg agttgcgttg tcagcgccgt atccagctgg gcagcgagtt cgttatagcg
    48121 ctgtaacccc agccgacgct taatcgccga aagtccgtga tgcaggtatt cgcaatattc
    48181 ctgccagccc tgttcgtcgc cataagcgtg ggtaaaatca tgatgcatac gcaagaaata
    48241 ctcttcattc aggcgttcaa gacggatctc aaacggatct tctaccaccg caatcgccgc
    48301 ctgagtcatt cgctcgcgca gacattccgg caggtgattc gaaccgatca tccggctttc
    48361 atcttccagc acccacaggc gcaaatcctg acgggcgtcg gtttttagca tttcggcagc
    48421 cagcaggttt tcaaaactcg cctggctaag ttgtggttgt aacgtgcgac caaacgccga
    48481 accgcgatga cgcgccagcc cttccagatc aacaccgttt ggctgttgtt gcactaatag
    48541 cgttttaccg ctgccggtac aaccgccaat cagcactatc ggtttttgtg ccagttcaat
    48601 ggtcgcctga atcgcggtct ggcgcagtgc cttataaccg ccttccacca gcggataatc
    48661 aatccccgct tcatgcagcc agcgttgcac aatatgtgaa cgttgaccgc cacgggcgca
    48721 gcagagaata ccttgcggat tttgcaggca cgctgcccgc caggcgtaca tacgctgctg
    48781 acgaatttca cccgccacca gtttatgccc cagcgccagc gctgcgtctg agccttgctg
    48841 tttatagcag gtgccaacgg cggcgcgttc atcgttattc attaacggca gattgatagc
    48901 ggcgggcatt gcgccttgct caaactcgat aggggcgcga acatcaataa tgggcgtatc
    48961 agcaatcagc agggcacgat agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga
    49021 acctcaaatc agcttgcgcg ctattttacg cgccaacgcg caaggaaact tgatttttaa
    49081 ctgcgtgggt tgccgaaaat ttctaaaaat ccgctgattt ccggcctgcg ttgggtaaac
    49141 aaggtcacaa tatcttctac cgctttgccg ctgccaaatt tgcgccatgc cagactcaat
    49201 ggcgaaggag ggcgcatcgt tgggattacc cggctgacca gttgttgatt atcgatcatt
    49261 gactggcaaa gtgattttgg caaaaaacca atgccaacac ccgccagatg ggcggcgatt
    49321 ttcgtttcca tatcgggaac aataatctct ttttgccccg gcaatcgcca ggcgacgcgt
    49381 ttggttaagg tgcgggcgct gtcttcaata ttgaccgccg gaaagcgacg caactgcgct
    49441 tctgttagcg gctcttcaac gttcgccagc ggatgatccg ccgccatgac aaatcgccat
    49501 tgcaccgatc ctaagggatc aagactaaag gtatttgcca gcgcctcagt tcccgtgacc
    49561 ccgatagcca gcgaaaaacc ttcgtacaat agcgagtccc agacgcccat atagatttgt
    49621 cgggagatgt gaaactgggt aaaggggtaa cgctcattca gccacgccag caactgggcg
    49681 acggcctggg ggttatagag caggttgttg atgacaatat tcacctggcg ttccacgcca
    49741 tcattcacct gttgcagctc gctgggcata ctttccagcc agctcagcca gtctctggcc
    49801 tgggaaagta gatgctcgcc agccgctgtc aacgttacgc tacgagtcgt acggaaaaac
    49861 agcgctactc cggtattctc ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt
    49921 ttacataatc gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc
    49981 cgcaaggttt ctggatcgaa catcttcagg tatccccttt taaatccgca agttgcgtga
    50041 ttttcttatc ctctgattta tcagtatttt tacatgatag ccctgttcaa tttgtggact
    50101 aaatctagtt ttggaaaaac attccaactt ttgtattgat gttgttctct taaggtttta
    50161 gattgcctgt tattgaaacc aagctgaccg gtcggcggtg gttgaacgga attatgttac
    50221 aaggacaaaa agatgaaact tcaggtatta ccgttaagtc aggaagcctt tagtgcttat
    50281 ggcgacgtaa tcgaaacgca gcaacgggat tttttccata ttaacaatgg cctggtggag
    50341 cgttaccacg atttggcgct ggttgagatt cttgagcaag accgtacgct tatcagcatt
    50401 aaccgcgcgc aaccggcgaa tctgccgctg accattcacg aactcgaacg tcatccgctg
    50461 ggcactcagg cctttatccc aatgaaaggt gaagtgtttg tggtggtcgt ggcgttaggc
    50521 gacgacaaac cagacctgtc aacgctgcgg gcgtttatca ccaacggtga acagggggtg
    50581 aattaccatc gtaacgtcta gcatcaccca cttttcgcct ggcagcgcgt caccgatttt
    50641 ctgaccatcg atcgcggcgg cagtgacaac tgtgatgttg aaagtattcc tgaacaggaa
    50701 ctcagttttg cgtgacgcct gcaaccgact tgcataagat aaactaattg ttcattgttt
    50761 atgctcactt gtaggtcgga gttaacgtag gtatgacgga agttagacgg cgcggcaggc
    50821 caggacaggc ggagcctgtg gcacagaagg gcgcacaggc gttagagcgg ggaattgcga
    50881 ttctgcaata tctggaaaaa agtgggggaa gttcgtcggt tagcgatatt tctctcaatc
    50941 tggatttgcc gctctccacg acctttcgct tgctgaaggt tttacaagca gcggattttg
    51001 tctatcagga cagtcaatta ggctggtggc atataggatt aggtgtcttt aacgtcggtg
    51061 cggcgtacat ccataaccgc gatgtcctct ccgtcgccgg gccgtttatg cgccgcctga
    51121 tgttactttc cggcgaaacg gtcaatgtcg cgatccgtaa cggcaatgaa gcggtattaa
    51181 ttggtcagtt agagtgtaaa tcgatggtca ggatgtgtgc gccactgggc agtcgtctgc
    51241 cgctgcatgc ttccggtgcg ggcaaagcgc tgctttatcc gctggcggaa gaggagttga
    51301 tgagcatcat tctgcaaacc ggtttgcagc agtttacgcc aactacgctt gtggatatgc
    51361 ccaccttgct gaaggacctg gaacaagcgc gtgaactggg ctataccgta gataaagaag
    51421 agcatgttgt aggtctgaat tgcatagctt cagcaattta cgatgatgtc ggtagcgttg
    51481 ttgccgctat ctccatctcc gggccttcat caagactgac agaagatcgt tttgtcagtc
    51541 agggtgagct ggtcagagac accgcccgcg atatcagcac ggcgttggga ctgaaagcac
    51601 atccatagtg tctgtcgcat cccgctctgc ggagcgggtt tttttgacaa aatttgaaag
    51661 ttggaaaatt tttccaatta atagaggtag gaataaaatg gcaaaaatga gagccgttga
    51721 cgcggcaatg tatgtgctgg agaaagaagg tatcaccacc gccttcggcg ttccgggagc
    51781 tgcaattaat ccgttctact cagcgatgcg taagcacggc ggtattcgtc acattctggc
    51841 gcgtcatgtg gaaggtgttt cgcacatggc ggaaggttat acccgcgcaa cggcagggga
    51901 tatcggcgta tgtctgggga cttccggtcc tgcgggcacg gacatgatca ccgcgctcta
    51961 ttccgcttct gctgattcca ttcctattct gtgcattacc ggccaggcac tgcgcgcccg
    52021 tctgcataaa gaagattttc aggccgtaga tattgaagca attgctaaac cggtcagcaa
    52081 aatggcggtt acagttcgtg aagcggcgct ggtgcctcgc gtgctgcaac aggcatttca
    52141 cctgatgcgt tctggtcgtc cgggtccggt actggtggat ttaccgttcg acgttcaggt
    52201 tgcggaaatc gagtttgatc ctgacatgta cgaaccgctg ccggtctaca aacctgctgc
    52261 cagccgtatg cagatcgaaa aagctgtaga aatgttaatc caggccgaac gtccggtgat
    52321 tgttgccggg ggcggggtaa tcaatgctga cgcagctgca ctgttacaac agtttgctga
    52381 actgaccagc gttccggtga tcccaacgct gatgggctgg ggctgtatcc cggacgatca
    52441 tgaactgatg gccgggatgg tcggtctgca aaccgcgcat cgttacggta acgcaacgtt
    52501 gctggcgtcc gacatggtgt ttggtatcgg taaccgtttt gctaaccgtc ataccggttc
    52561 ggtagagaaa tacaccgaag ggcgcaaaat cgttcatatt gatattgagc cgacgcaaat
    52621 tggtcgcgtg ctgtgtccgg atctcggcat tgtctctgat gctaaagcgg cgctgacact
    52681 gctggttgaa gtggcgcagg agatgcaaaa agcgggtcgt ctgccgtgtc gtaaagaatg
    52741 ggtcgccgac tgccagcagc gtaaacgcac tttgctgcgc aaaacccact tcgacaacgt
    52801 gccggtgaaa ccgcagcgcg tgtatgaaga gatgaacaaa gcctttggtc gcgatgtttg
    52861 ttatgtcacc actattggtc tgtcacaaat cgctgcggca caaatgctgc atgtctttaa
    52921 agaccgccac tggatcaact gtggtcaggc tggtccgtta ggctggacga ttccggcggc
    52981 gctgggggtt tgtgccgctg atccgaaacg caatgtggtg gcgatttctg gcgactttga
    53041 cttccagttc ctgattgaag agttagccgt tggcgcgcag ttcaacattc cgtacatcca
    53101 tgtgctggtg aataacgcct atctcggcct gattcgtcag tcacaacgcg cttttgacat
    53161 ggactactgc gtgcaactcg ctttcgagaa tatcaactcc agtgaagtga atggctacgg
    53221 tgttgaccac gtaaaagtag cggaaggttt aggttgtaaa gctattcggg tcttcaaacc
    53281 ggaagatatt gcgccagcct ttgaacaggc gaaagcctta atggcgcaat atcgggtacc
    53341 ggtagtcgtg gaagttattc tcgagcgtgt gaccaatatt tcgatgggca gcgaactgga
    53401 taacgtcatg gaatttgaag atatcgccga taacgcagcg gatgcaccga ctgaaacctg
    53461 cttcatgcac tatgaataag ggagataaat gatgttacgt ttctctgcta atttatcgat
    53521 gttatttggt gaatatgatt ttctcgcccg ttttgagaaa gctgcgcagt gtggttttcg
    53581 cggcgttgaa tttatgtttc cttatgacta cgacattgaa gaattaaaac aggtactggc
    53641 gagtaataaa ctcgaacata cgctgcacaa tttaccggca ggtgactggg cggcgggcga
    53701 gcgcggtatt gcctgtattc ctggccgtga agaagagttt cgggatggcg tagcagcagc
    53761 gattcgttat gcccgtgcgc tgggtaataa aaaaattaac tgtctggtcg gtaaaacgcc
    53821 ggctggtttc agcagtgaac agattcacgc aacgcttgta gaaaacctgc gttatgccgc
    53881 gaatatgctg atgaaagaag atattttatt actgattgaa cctattaacc attttgatat
    53941 tcttggtttc catctcaccg gaactcggca ggcgctgaaa ttgattgatg atgttggttg
    54001 ctgcaatttg aaaattcagt atgacattta tcacatgcag cggatggaag gtgaattaac
    54061 caacaccatg actcagtggg ccgataaaat tggtcacctg caaattgctg ataatccgca
    54121 tcgcggcgaa ccgggaaccg gagaaattaa ttacgaatat ctctttacgg taattgaaaa
    54181 ctctgactac aacggttgga ttgggtgtga atataaaccc caaaccacca cggaagctgg
    54241 tttacgctgg atggatccgt accgttaaaa cgtaacgcta ttcagacaat gcttttttag
    54301 gccgctaagt tggcagggga tcgtgttgtc tgaattcagg aaaagcgaaa tttaaaagag
    54361 gttaattatg aaactgggat ttattggctt aggcattatg ggtacaccga tggccattaa
    54421 tctggcgcgt gccggtcatc aattacatgt cacgaccatt ggaccggttg ctgatgaatt
    54481 actgtcactg ggtgccgtca gtgttgaaac tgctcgccag gtaacggaag catcggacat
    54541 catttttatt atggtgccgg acacacctca ggttgaagaa gttctgttcg gtgaaaatgg
    54601 ttgtaccaaa gcctcgctga agggcaaaac cattgttgat atgagctcca tttccccgat
    54661 tgaaactaag cgtttcgctc gtcaggtgaa tgaactgggc ggcgattatc tcgatgcgcc
    54721 agtctccggc ggtgaaatcg gtgcgcgtga agggacgttg tcgattatgg ttggcggtga
    54781 tgaagcggta tttgaacgtg ttaaaccgct gtttgaactg ctcggtaaaa atatcaccct
    54841 cgtgggcggt aacggcgatg gtcaaacctg caaagtggca aatcagatta ttgtggcgct
    54901 caatattgaa gcggtttctg aagccctgct atttgcttca aaagccggtg cggacccggt
    54961 acgtgtgcgc caggcgctga tgggcggctt tgcttcctca cgtattctgg aagttcacgg
    55021 tgagcgcatg attaaacgca cctttaatcc gggcttcaaa atcgctctgc accagaaaga
    55081 tctcaacctg gcactgcaaa gtgcgaaagc acttgcgctg aacctgccaa acactgcgac
    55141 ctgccaggag ttatttaata cctgtgcggc aaacggtggc agccagttgg atcactctgc
    55201 gttagtgcag gcgctggaat taatggctaa ccataa
//
LOCUS       DADKXM010000012        52229 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.012, whole genome shotgun sequence.
ACCESSION   DADKXM010000012 DADKXM010000000
VERSION     DADKXM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.012"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(12..911)
                     /gene="nhaR"
                     /locus_tag="JQR05_000723"
     CDS             complement(12..911)
                     /gene="nhaR"
                     /locus_tag="JQR05_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY5322955.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(977..2143)
                     /gene="nhaA"
                     /locus_tag="JQR05_000724"
     CDS             complement(977..2143)
                     /gene="nhaA"
                     /locus_tag="JQR05_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY5322956.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2672..2881
                     /gene="mokC"
                     /locus_tag="JQR05_000725"
     CDS             2672..2881
                     /gene="mokC"
                     /locus_tag="JQR05_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY5322957.1"
                     /translation="MLNTCRVPLTDRKVKGKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(2985..4115)
                     /gene="dnaJ"
                     /locus_tag="JQR05_000726"
     CDS             complement(2985..4115)
                     /gene="dnaJ"
                     /locus_tag="JQR05_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY5322958.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4204..6120)
                     /gene="dnaK"
                     /locus_tag="JQR05_000727"
     CDS             complement(4204..6120)
                     /gene="dnaK"
                     /locus_tag="JQR05_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY5322959.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6497..6901
                     /locus_tag="JQR05_000728"
     CDS             6497..6901
                     /locus_tag="JQR05_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY5322960.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6927..7640
                     /gene="msyB"
                     /locus_tag="JQR05_000729"
     CDS             6927..7640
                     /gene="msyB"
                     /locus_tag="JQR05_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY5322961.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLHGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7789..8355
                     /gene="satP"
                     /locus_tag="JQR05_000730"
     CDS             7789..8355
                     /gene="satP"
                     /locus_tag="JQR05_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY5322962.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNITGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8390..8977)
                     /gene="mog"
                     /locus_tag="JQR05_000731"
     CDS             complement(8390..8977)
                     /gene="mog"
                     /locus_tag="JQR05_000731"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY5322963.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLASALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9092..10045)
                     /gene="tal"
                     /locus_tag="JQR05_000732"
     CDS             complement(9092..10045)
                     /gene="tal"
                     /locus_tag="JQR05_000732"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY5322964.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10324..11754
                     /locus_tag="JQR05_000733"
                     /pseudo
     CDS             10324..11754
                     /locus_tag="JQR05_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            11824..12600
                     /gene="yaaA"
                     /locus_tag="JQR05_000734"
     CDS             11824..12600
                     /gene="yaaA"
                     /locus_tag="JQR05_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY5322965.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12753..13049)
                     /locus_tag="JQR05_000735"
     CDS             complement(12753..13049)
                     /locus_tag="JQR05_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY5322966.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13263..14549)
                     /gene="thrC"
                     /locus_tag="JQR05_000736"
     CDS             complement(13263..14549)
                     /gene="thrC"
                     /locus_tag="JQR05_000736"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY5322967.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(14550..15482)
                     /gene="thrB"
                     /locus_tag="JQR05_000737"
     CDS             complement(14550..15482)
                     /gene="thrB"
                     /locus_tag="JQR05_000737"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY5322968.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15484..17946)
                     /gene="thrA"
                     /locus_tag="JQR05_000738"
     CDS             complement(15484..17946)
                     /gene="thrA"
                     /locus_tag="JQR05_000738"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY5322969.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(18027..18092)
                     /gene="thrL"
                     /locus_tag="JQR05_000739"
     CDS             complement(18027..18092)
                     /gene="thrL"
                     /locus_tag="JQR05_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY5322970.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18306..18992)
                     /locus_tag="JQR05_000740"
     CDS             complement(18306..18992)
                     /locus_tag="JQR05_000740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY5322971.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19392..19532)
                     /locus_tag="JQR05_000741"
     CDS             complement(19392..19532)
                     /locus_tag="JQR05_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5322972.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19628..20344
                     /gene="arcA"
                     /locus_tag="JQR05_000742"
     CDS             19628..20344
                     /gene="arcA"
                     /locus_tag="JQR05_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY5322973.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20403..21755)
                     /gene="creD"
                     /locus_tag="JQR05_000743"
     CDS             complement(20403..21755)
                     /gene="creD"
                     /locus_tag="JQR05_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY5322974.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(21813..23237)
                     /gene="creC"
                     /locus_tag="JQR05_000744"
                     /pseudo
     CDS             complement(21813..23237)
                     /gene="creC"
                     /locus_tag="JQR05_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
     gene            complement(23237..23926)
                     /gene="creB"
                     /locus_tag="JQR05_000745"
     CDS             complement(23237..23926)
                     /gene="creB"
                     /locus_tag="JQR05_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY5322975.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(23939..24412)
                     /gene="creA"
                     /locus_tag="JQR05_000746"
     CDS             complement(23939..24412)
                     /gene="creA"
                     /locus_tag="JQR05_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY5322976.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24623..25492
                     /gene="robA"
                     /locus_tag="JQR05_000747"
     CDS             24623..25492
                     /gene="robA"
                     /locus_tag="JQR05_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY5322977.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25489..26136)
                     /gene="gpmB"
                     /locus_tag="JQR05_000748"
     CDS             complement(25489..26136)
                     /gene="gpmB"
                     /locus_tag="JQR05_000748"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY5322978.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26188..26709
                     /gene="yjjX"
                     /locus_tag="JQR05_000749"
     CDS             26188..26709
                     /gene="yjjX"
                     /locus_tag="JQR05_000749"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY5322979.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(26794..27120)
                     /gene="trpR"
                     /locus_tag="JQR05_000750"
     CDS             complement(26794..27120)
                     /gene="trpR"
                     /locus_tag="JQR05_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY5322980.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27210..29147)
                     /gene="sltY"
                     /locus_tag="JQR05_000751"
     CDS             complement(27210..29147)
                     /gene="sltY"
                     /locus_tag="JQR05_000751"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY5322981.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAR
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDGIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29358..31025
                     /gene="ettA"
                     /locus_tag="JQR05_000752"
     CDS             29358..31025
                     /gene="ettA"
                     /locus_tag="JQR05_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY5322982.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31333..32565)
                     /gene="nadR"
                     /locus_tag="JQR05_000753"
     CDS             complement(31333..32565)
                     /gene="nadR"
                     /locus_tag="JQR05_000753"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY5322983.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     IRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32586..33968)
                     /gene="radA"
                     /locus_tag="JQR05_000754"
     CDS             complement(32586..33968)
                     /gene="radA"
                     /locus_tag="JQR05_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY5322984.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34017..34985)
                     /gene="serB"
                     /locus_tag="JQR05_000755"
     CDS             complement(34017..34985)
                     /gene="serB"
                     /locus_tag="JQR05_000755"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY5322985.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35091..35735
                     /locus_tag="JQR05_000756"
     CDS             35091..35735
                     /locus_tag="JQR05_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY5322986.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35763..36779
                     /gene="lplA"
                     /locus_tag="JQR05_000757"
                     /pseudo
     CDS             35763..36779
                     /gene="lplA"
                     /locus_tag="JQR05_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            36811..37074
                     /locus_tag="JQR05_000758"
     CDS             36811..37074
                     /locus_tag="JQR05_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5322987.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37235..37954)
                     /gene="deoD"
                     /locus_tag="JQR05_000759"
     CDS             complement(37235..37954)
                     /gene="deoD"
                     /locus_tag="JQR05_000759"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY5322988.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38011..39234)
                     /gene="deoB"
                     /locus_tag="JQR05_000760"
     CDS             complement(38011..39234)
                     /gene="deoB"
                     /locus_tag="JQR05_000760"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY5322989.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39286..40608)
                     /gene="deoA"
                     /locus_tag="JQR05_000761"
     CDS             complement(39286..40608)
                     /gene="deoA"
                     /locus_tag="JQR05_000761"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY5322990.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(40735..41514)
                     /gene="deoC"
                     /locus_tag="JQR05_000762"
     CDS             complement(40735..41514)
                     /gene="deoC"
                     /locus_tag="JQR05_000762"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY5322991.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTNEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41772..43313
                     /locus_tag="JQR05_000763"
     CDS             41772..43313
                     /locus_tag="JQR05_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY5322992.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIVPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDEIIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43285..44148
                     /gene="yjjW"
                     /locus_tag="JQR05_000764"
     CDS             43285..44148
                     /gene="yjjW"
                     /locus_tag="JQR05_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY5322993.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNGCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKICPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44362..45144)
                     /locus_tag="JQR05_000765"
     CDS             complement(44362..45144)
                     /locus_tag="JQR05_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5322994.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45141..46214)
                     /locus_tag="JQR05_000766"
     CDS             complement(45141..46214)
                     /locus_tag="JQR05_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY5322995.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46336..46497)
                     /locus_tag="JQR05_000767"
     CDS             complement(46336..46497)
                     /locus_tag="JQR05_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY5322996.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46624..47229)
                     /gene="osmY"
                     /locus_tag="JQR05_000768"
     CDS             complement(46624..47229)
                     /gene="osmY"
                     /locus_tag="JQR05_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY5322997.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47622..49211)
                     /gene="prfC"
                     /locus_tag="JQR05_000769"
     CDS             complement(47622..49211)
                     /gene="prfC"
                     /locus_tag="JQR05_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY5322998.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49302..49979)
                     /gene="yjjG"
                     /locus_tag="JQR05_000770"
     CDS             complement(49302..49979)
                     /gene="yjjG"
                     /locus_tag="JQR05_000770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY5322999.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(49994..50440)
                     /gene="rimI"
                     /locus_tag="JQR05_000771"
     CDS             complement(49994..50440)
                     /gene="rimI"
                     /locus_tag="JQR05_000771"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY5323000.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50409..50822)
                     /gene="holD"
                     /locus_tag="JQR05_000772"
     CDS             complement(50409..50822)
                     /gene="holD"
                     /locus_tag="JQR05_000772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY5323001.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            50925..51956
                     /gene="rsmC"
                     /locus_tag="JQR05_000773"
     CDS             50925..51956
                     /gene="rsmC"
                     /locus_tag="JQR05_000773"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY5323002.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12395 a        14171 c        13111 g        12552 t
ORIGIN      
        1 ggagctgccg attaacgcac cgctggacta aaaagcgcag aataatccgt attgcagatt
       61 cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa tagcatggta ctcttccatc
      121 acattctcga cgcgaccaat ttctacgaca gttttatcgg cataaaagtc atatgcataa
      181 agcgttgggg caacgaagat tgcattgtgc atcgcaccaa aagctttcat caaagcggca
      241 tcatcaaact cgccgaggat ttctacgttt aatccctggg agttaaacca gttaagcaat
      301 ttgcgcccta acattgaacg tcgcccagga atcaaaagtc gccgttcttc cagacaagcc
      361 gggaacggtt tttctggtgg tggatttgta caccagaaac tcacgccaca ttcgccaatt
      421 ctcacggaga acaggccttc ctgctgcgta gagtctatcg gacagtcaga aatgatcata
      481 tccagtttat gctgacttaa ttgctccagc agcatttcgt gggtggattc gaagcagcga
      541 agatgaatgg gctcgccttc taccactgcg gcgttaagta cgctactgac caggcgtttg
      601 gaaagtgcat cagccacgcc aacgtcaaac aataaattgg attctttgcg atagttcaca
      661 atatccagca tttcctggct taaggtgaac attttatcgg catagcgata gaccagttct
      721 cccagctcgc tgggttcgag accacgtccc ttgcgtttaa ataatttgcc ttgcaggcgc
      781 tcttccagcg ctcgaatctg tccggtaatg gtttgtggag ttaaataaag cgcctccgct
      841 gcgccaacca cggaaccttc tttatagaca tgccagaagt aatacaagtg gttgtaattg
      901 atatgagaca tgctcatttc tctccctgat aacaatgaaa agggagccgt ttatggctcc
      961 ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac gcgtaaccag ctgtatccaa
     1021 ttaccgccga agagatagaa ccgaccagga taccgagttt cgcccagtta atcagttctg
     1081 gatctacgct accaaaggcc aggctggcaa taaagataga catagtaaaa ccgataccgc
     1141 acaggatccc caccaccata atttgctgat aagtcgttcc ctcaggcaga tgcgccagtt
     1201 tcaaacgcag cgccaaccag cagaacagac taatccccag cggtttgcca atcagcaagc
     1261 cagcgatgat ccccaatggc agaatggagg tcaagccatc cagcgtgacg ccttgcagtg
     1321 aaacgccagc attagcaaat gcaaacagcg gcaaaatcag atacgccacc cacgggtgca
     1381 acacatgctc cagtcgcttc gctggagaac gcccatgctt ctctttcaaa ggaataaaga
     1441 agccgacaat tacccccgcc agagttgcgt gaacccccga tttcaacacc gcagtccaca
     1501 acaccacgcc aacaagaata tagacgcccg tgcggcgtac accacacaga ttcaataccg
     1561 cgagtaccgc aattgctaca gccgcgacgc caagagaggc catcgataag tcattagtgt
     1621 agaacaatgc gatgataatg atggccccaa gatcgtcgat aatagccaga gccatcaaaa
     1681 agatcttcag cgctaacgga acacgacttc ccaacagcgc cagtacacca agtgcaaaag
     1741 caatgtcagt agccgccggg atcgcccacc cttcgcgggt aatcggatcg gcatagttaa
     1801 aagccagata gagtaatgcc ggcacaatca tcccaccaat agcggcgata actggaaatg
     1861 cggcctggcg taagctggct agcgatcctt gcatcagttc acgtttaact tccagaccga
     1921 ctaacaggaa aaataccgcc atcagcgcgt catttatcca taacagcatg tttttgttga
     1981 tttcgagtga accaacccgg agctgaaccg gcgtctccag aaagtcgtga taccatccac
     2041 tggttgcgcc gctgttggcc ataatcatcg ccaggatagc ggcaatgata agaataatgc
     2101 ctcccgaggc atcactgcta aagaatcgat gcagatgttt cactttttat ttctctttca
     2161 ggtgaataga tcgtttttac tattttaccc cgcacgaatc atcgttaaaa tcagattata
     2221 gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt tgcgccatca gcggtgagat
     2281 gcgtttttga cggctgtaag cgctcacgag tgaccctaag cccagataat catagcttaa
     2341 ggtgaggatt cctgagggat taattaggaa aagtaataat taatttgttg tcagtcggcg
     2401 atgcttgtta ggccaatatt tcaaaaataa tccagcttgt cgtcaataat tgcgatgaag
     2461 agataaatta aagttaaata attaaatcat taaaaaatta cgccacaaaa atgcgaggcg
     2521 tcttcaggat tagaatcgat aaaacaaaat gggttaaaga gagcgatata atagcggcgg
     2581 gtgcttgagg ctgtctgtct caggcattag ctgaacggca gatagagaaa agccccgagt
     2641 gatattttac catcaacccg aggcctccta tatgctgaac acatgtagag tgcctcttac
     2701 tgaccgtaag gtcaagggga agagagcaat gaagcagcat aaggcgatga ttgtcgccct
     2761 gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag acctctgtga
     2821 ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg aatccgagta
     2881 agagcaacgg cggggaggga tccccgccac tttacaggtg ctcgcatatc ttcaacgcac
     2941 cctattttta cccaggcctg cccacgggca ggcttttggg gaggttagcg ggtcaggtcg
     3001 tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg
     3061 gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt ttcgttcagg
     3121 cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc accgcggaca
     3181 gacttgacgc ctttaccgcg catacggaat agcttaccgg tctgggtttc gccaggcact
     3241 ttcagtttga cgcgaccatc aagggtcggt acttcgattt cgccacccag cgccgccata
     3301 gcgaagttga tcgggacttc gcaatacagg ttgttgcctt cacgctcgaa aatcgggtgc
     3361 tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccatgctc gcccgcttca
     3421 ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat tttaacggac
     3481 agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg atctttgatc
     3541 agcgtaccgc ggccctgaca gtgtggacag gtctgctgta cagcgaagaa tccctggcgc
     3601 atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt acctggtttt
     3661 gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat ctctttggtc
     3721 acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc agcaccgcgc
     3781 gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata
     3841 tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca tgccaccttg ctcaaacgca
     3901 gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac ttcataagct
     3961 tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg gtccgggtgg
     4021 tatttcatgg ccagccgttt gtaggccttt ctgatttcac gctcttccgc tgttttggaa
     4081 acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc cctagatgaa
     4141 tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc gtttataggg
     4201 cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt catctttcgc
     4261 gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct gctgggcgat
     4321 ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt ttcgcttcga tagcggcttt
     4381 gtcttcacct ttcagagcag tttccagtgc agtcagcgca gactcgatag cagttttgtc
     4441 gtcagccggc agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt gcagcagatg
     4501 gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt cggcgttagc
     4561 ttctgcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag aagccttgat
     4621 ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca ggataccgtc
     4681 agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg ggttgatacc
     4741 atctaggttg aactgaccca gagatttgtt atcagccgca cgtttacgtt caccctgcag
     4801 cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct ggctgtgctt
     4861 ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat
     4921 acccagagac agcggggtaa cgtccagcag cagtacgtct tttacgtcac cagtcagaac
     4981 accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt ctttacgcgg
     5041 ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag tctgaccacc
     5101 aacgaggata acgtcgtcga tatcagatac ggacaggcca gcatcctgca gtgcaacttt
     5161 cagcggctca atggaacggt ttaccagatc ttcaaccagg ctttccagtt tcgcacgagt
     5221 cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtatg gcaggttaac
     5281 gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt ctttcaggcg
     5341 ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga attcttcaac
     5401 cagatagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt
     5461 tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa tagaaatatc
     5521 gaaagtacca ccccccaggt cataaaccgc gatagtacgg ttgccagtgc ctttgtccag
     5581 accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt ccagaccagc
     5641 gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt
     5701 gataacagct tcagttaccg gttcacccag gtaatcttca gcggttttct tcattttttt
     5761 cagcacttca gcagaaatct gcggcggtgc cattttctgg cctttaactt cgacccatgc
     5821 gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac gctgtacttc
     5881 ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt tttgcgggtt
     5941 cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct gggtataggc
     6001 aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc agcacgcgag gagtggtgcc
     6061 atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta ttttacccat
     6121 ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa
     6181 acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg cggttgacta
     6241 ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg
     6301 cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt gacagcaatt ttattcatat
     6361 aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt aataaaaaga aaagattacg
     6421 tgcctgaatc ttctctttat cagcagtaaa ctagtgggta ttcatccccc tacctcttcc
     6481 cactaagaga atccttatga aatccgtttt tacgatttcc gccagcctgg cgattagcct
     6541 gatgctgtgc tgcacggcgc aggcaaacga ccataaactc ctcggcgtca ttgcaatgcc
     6601 gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt tgtcgcattg tgaaacgcat
     6661 acaactctct gccgaccatg gcgatttaca gttaagcggt gcatcggttt atttcaaagc
     6721 cgcccgtagc gccagtcaga gcctgaatat tccttcagaa ataaaagaag ggcaaaccac
     6781 tgactggatc aacattaaca gcgataacga caataaacgc tgcgtctcaa aaatcacctt
     6841 ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg gcgacgacta
     6901 acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat tcagatctgg
     6961 attttcttca gcattgtagt gaggagcagt tggcaaattt cgcccgattg ctcacccata
     7021 atgaaaaagg caaaactcgc ctctccagcg tactgatgcg caacgaactg tttaaatcga
     7081 tggaagggca tcccgagcaa catcgccgca actggcagct gattgccgga gaattacagc
     7141 attttggtgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg tatcgggcca
     7201 ttttgctcga tgtctcaaag cgattgaagc tgaaagccga caaagagatg tctacgtttg
     7261 aaattgagca acagttactg gaacaatttc tgcgtaatac ctggaagaaa atggacgagg
     7321 aacataagca ggagtttctg cacgcggtcg atgccagggt gaatgagctg gaagagctgc
     7381 tgccgctgct gatgaaagac aaattattgg caaaaggtgt gtcgcatttg ctttccagcc
     7441 aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat ggtttgctgc
     7501 acggcgcggg gctgggaggc cctgtgggcg cagcattaaa tggggttaaa gcggtcagcg
     7561 gcagcgccta tcgcgtgacg attccagccg tactgcaaat cgcctgcctg cgccggatgg
     7621 ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc ccctcaaaag
     7681 atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctacagttat
     7741 tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact
     7801 aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt
     7861 aacctgcaca acgtgggtta tttcgctctg gacggtatta ttcttgccat gggcattttc
     7921 tacggcggca tcgcgcaaat ttttgctggt ctgctggagt acaaaaaagg caacactttc
     7981 ggtttaaccg cattcacctc ttacggttct ttctggctga cgctggttgc gattctgctg
     8041 atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt
     8101 ctgtggggcg tatttacgct gtttatgttc ttcggcactc tgaaaggcgc acgcgtcctg
     8161 caattcgttt tctttagcct gaccgtgctg tttgccctgc tggcgatcgg taacattacc
     8221 ggtaatgccg cgatcatcca ctttgccggc tggattggtc tgatctgcgg tgccagcgca
     8281 atctatctgg cgatgggtga agtgctgaac gagcagtttg gtcgcaccgt tctgccgatt
     8341 ggtgaatccc actaattgtc ttgagatccc cccgctcggg gggatttttt tattcgctaa
     8401 cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt
     8461 atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaatacca tgcaccacaa
     8521 cgttaccctc agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg
     8581 gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac ctgacgcgaa aggatcgcag
     8641 ttggtacaaa atgcaggctg atctggcgca tctgttcacc aaagccaggc atctcgcggt
     8701 ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg
     8761 tggtgagcac cagatggcaa ctcatttcat ccaccagctc acacaacgtt tgctcgatga
     8821 tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg
     8881 ccagccattc ttccagcgct ggaatgcctt tatcctgata aacgccgctg gatgcgcgat
     8941 cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt
     9001 ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc
     9061 gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca
     9121 gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg
     9181 gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca
     9241 cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctccgcc agctctttca
     9301 gcagtgccgg tgcgatggtc agacggtcac agcctgccag ttccagaatt tcgccgatgt
     9361 tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga
     9421 tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcggtat
     9481 tcgctttgta ccagtcaaga atacggccaa caaacggcga gatcaggaac acgcccgcct
     9541 ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag ttgatacctt
     9601 ctttttccag ctgttctgca gcacggatac cctgccaggt agaagccagt ttgatcagaa
     9661 tacgatcgtt gctaatacct gcatcgttgt agagtttgat caggcgtttt gctttcgcaa
     9721 ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc agttgagata cggcccggaa
     9781 ccagtttcag gatttccaga ccaatattta ctgccagttt gtcggtcgcg tccacgatct
     9841 gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac
     9901 ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg
     9961 gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac
    10021 gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga
    10081 tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg
    10141 gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt
    10201 ttatacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact
    10261 tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata
    10321 tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac
    10381 ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac
    10441 atccgccagt ttggcaaaag tcttaaaaat agcattcatc catagccagg cggtttaacc
    10501 tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc
    10561 gttgcgctgg caattaccgc cggtggacca ggtgccgtct tttggatgtg ggttgctgcg
    10621 tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta taaagaacgt
    10681 gacgtcaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg gctggggatg
    10741 cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc
    10801 ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt cttttgattt tcctccgctg
    10861 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
    10921 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt actgaccagc
    10981 ctggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
    11041 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
    11101 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg atcgacgcca
    11161 aacgcggcag cggcagctgc gtcctggcct ccgcatccgg cagcacaagg gattgtccag
    11221 atgattggca tttttatcga caccctggtt atctgtacgg caagcgccat gctgatatta
    11281 ctggcgggta acggcacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
    11341 atgcgggtgc taatgggttt ctggggtgct gaatttgtta ccctcgtggt tattctgttt
    11401 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
    11461 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
    11521 ggcaccttgc taagccttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
    11581 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
    11641 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
    11701 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
    11761 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
    11821 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
    11881 acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca tgaagcgcgg
    11941 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
    12001 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc ccgccaggcg
    12061 attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac
    12121 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
    12181 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
    12241 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    12301 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    12361 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    12421 aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    12481 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    12541 tttgatgaag attcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    12601 gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    12661 ttcaatatat tgcagaatta acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    12721 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    12781 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    12841 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    12901 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    12961 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    13021 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    13081 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    13141 ctaatttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    13201 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    13261 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    13321 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    13381 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    13441 tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc
    13501 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    13561 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    13621 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    13681 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    13741 ataaaacgtt tcaccggcag accgaatgac ttcgccagca gacccgccgt caaatcgccg
    13801 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cctcctgcgg cagctgcgca
    13861 acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    13921 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    13981 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    14041 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    14101 ttcggcaaac cgtagaaagc atgagccact gcagctccgg tatcaccgga ggtcgcggtc
    14161 agaatggtca ctggcttatc atccgcaata tgggtcagca tttgtgccat aaagcgaccg
    14221 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    14281 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    14341 tcctgtggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    14401 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa
    14461 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    14521 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    14581 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    14641 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    14701 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    14761 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    14821 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    14881 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    14941 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    15001 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    15061 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacga
    15121 gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    15181 caggcgctgg agcctaagcc cgaaccgatc ggcatattct tttccagagt catcgccact
    15241 ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
    15301 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    15361 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgc caccgccgcc
    15421 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    15481 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
    15541 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    15601 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    15661 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    15721 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    15781 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    15841 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaatcaatag
    15901 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    15961 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    16021 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    16081 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    16141 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    16201 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    16261 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    16321 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    16381 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    16441 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    16501 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    16561 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    16621 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    16681 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    16741 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
    16801 cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgtgg
    16861 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    16921 ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca accatccctt tcatccccgg
    16981 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    17041 gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    17101 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    17161 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    17221 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    17281 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttacgtcc
    17341 aagcaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca acaccatgtg
    17401 atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    17461 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
    17521 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    17581 cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat gcaggacatg
    17641 ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg ggaaccccgg
    17701 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    17761 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    17821 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    17881 atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    17941 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
    18001 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg
    18061 gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc
    18121 tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt
    18181 aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt tttttaatcc
    18241 acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc
    18301 gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa cattgccgtg
    18361 tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt cgaccagttt tatgtcatct
    18421 gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg cagttgatgt
    18481 tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa ttgatagcaa
    18541 tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat cggcacacca
    18601 gtaagcacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc gcgaccaaac
    18661 accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac cagttctact
    18721 ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag tggtggcgac agtgaaatcg
    18781 acatcgtgta acgattcagc cagtgtcggg aaaactttaa tattatcaat aatatcacca
    18841 gatccatgtg cgacccagcg ggtggctggc tccaggtgtg cctgactatc gacaatccgc
    18901 agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt ttctgctctg
    18961 gcgggtgcga ccaggataat cgttatacgc atattgccac tcttcttgac caaataaccg
    19021 cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt cttaacgtaa
    19081 gccgcagaaa aagcccttta cttagcttaa aaaaggctaa actattttct gactgcacta
    19141 acggtttaat tgttaaaaaa tgctacatat ccttctgttt acttaggata attttataaa
    19201 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
    19261 ttattaaaat tatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
    19321 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
    19381 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
    19441 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
    19501 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
    19561 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
    19621 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
    19681 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
    19741 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
    19801 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
    19861 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
    19921 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
    19981 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
    20041 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
    20101 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
    20161 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccgcacga
    20221 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
    20281 gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg atctggaaga
    20341 ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    20401 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    20461 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    20521 accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag cagtccccac
    20581 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    20641 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    20701 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    20761 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    20821 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    20881 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc
    20941 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    21001 tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt tggctggtag aaaatcacct
    21061 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    21121 accacagaaa gatcgcctgt accgcttaaa ttcagtgcca tattcagctg caggttctgc
    21181 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    21241 gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac cacaccaata
    21301 ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat atttggcgcg
    21361 ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    21421 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    21481 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    21541 tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac gagtttttgc
    21601 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    21661 acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacaag
    21721 gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc aatggcagca
    21781 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgtagc
    21841 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    21901 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    21961 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    22021 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatgcaaccg
    22081 ctctcgggag taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    22141 gccggttcag cagcaacatt aacctcggag ggcataacat gcaaagtgat gtttttttct
    22201 gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac atcaacagca
    22261 gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagc
    22321 gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    22381 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    22441 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    22501 atactttcca gcgcctacgc gagtttacgc agctcgctac taccgagatc gggaagagga
    22561 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgagcaat ggagcggttg
    22621 atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccaacaaaat ggcgctggcc
    22681 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    22741 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    22801 gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg accacgcaac
    22861 gttagccaga cgtcattcca gcgcgaataa tcctgtccaa cggctttatt tgccgaatcg
    22921 aacaatactt tgccatgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    22981 atgccaccaa tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    23041 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc aagtaacgtt
    23101 gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    23161 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc ccgcagcaac
    23221 cgcatgccga tacgcattac aagcccctca ggctatatcc catgccgcga tgagtattaa
    23281 tcggtgaaag atcggggttg atggcgcgca gcttggcacg cagcgtttta atgtggatat
    23341 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    23401 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    23461 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    23521 gaccaatacg gatgacggga gacggcgtcg agaacttctt cacccgacgc agtaaggtgc
    23581 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    23641 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    23701 acgccggatg gagcgccagt aactggcggc acaattcaaa gccactgata tcaggcaaac
    23761 caacgtcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaaac
    23821 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    23881 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    23941 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    24001 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    24061 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    24121 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatggccg
    24181 catcggaggt atcttctgcc aggcccaatc cccctttaat accaccggtt ttcgcccggc
    24241 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    24301 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    24361 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    24421 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    24481 caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    24541 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    24601 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    24661 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    24721 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    24781 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    24841 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    24901 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    24961 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    25021 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    25081 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    25141 cattccgccg gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    25201 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    25261 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    25321 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaatct
    25381 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    25441 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    25501 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcagtttcaa cgacccagcc
    25561 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    25621 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    25681 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    25741 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    25801 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    25861 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    25921 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    25981 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    26041 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    26101 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    26161 acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac caccaatccc
    26221 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    26281 attgcatccg ttgccgtcga gagcggtgta ccggaacaac cctttggcag tgaggaaacg
    26341 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    26401 tgggtggcga ttgaagctgg cattgatggc gacagcactt tcagttgggt ggtgattgaa
    26461 aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc cgttacctgc ggtgattctg
    26521 gaaaaagttc gtgaaggcga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    26581 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    26641 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    26701 gcattgtagg acggataagg cgttcacgcc gcatccgaca ttcggtgcac gatgcctgat
    26761 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    26821 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    26881 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    26941 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    27001 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    27061 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    27121 aatatgtcgc cattgttagc gggggaagca aaatgactcc cccggttgta ctcgctaaag
    27181 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    27241 atcaacgtcg gtttatcccc catgaaatag cggtagtaag catcataagc cagcacgttc
    27301 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    27361 cgcccggcgc tgtggccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    27421 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    27481 atgttgatgt tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    27541 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    27601 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    27661 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    27721 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    27781 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    27841 ttcaccagat tggcccactc gctacgcgcg gtattatcga gattccagta catcaactcg
    27901 cgcacgcgcg ccatctccgg cccctgagtc agggtgctgt caacattctg cggcgcttta
    27961 tcaatcttca gctcatactc ttcaccgatg cgttgtgctg caaccatcgg gtagaaacca
    28021 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    28081 aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    28141 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg cacacggcgt
    28201 tcaatcaacg acgtggattg cgagcgcata atgccgtcat cgcgccattt cgcctgctca
    28261 tcggtgacat cattgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    28321 tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc attttcggca
    28381 tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa atcggtcgcg
    28441 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    28501 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac taggcctgtg
    28561 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    28621 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    28681 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    28741 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    28801 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    28861 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcagg
    28921 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    28981 atcatttgtt cgaccacatc catctgtcga ttatccctgg cctgcttgat ttgcgcgtaa
    29041 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    29101 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    29161 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    29221 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    29281 gggattaggc ggggaaacag gctacacttc gcctttgaaa actcatcatc acgataaaca
    29341 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    29401 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    29461 ttggtgtcct gggtctgaac ggcgcgggta agtccaccct gctgcgcatt atggcgggca
    29521 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttatctgc
    29581 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    29641 cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    29701 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    29761 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg cgtctgccag
    29821 attgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc
    29881 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    29941 ccgaatccgt ggcgtggctg gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga
    30001 ttactcacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa ctggaccgcg
    30061 gtgaaggtat tccgtgggaa ggtaactact cctcctggct ggagcagaaa gatcagcgtc
    30121 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    30181 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    30241 aagagctgaa cagcaccgaa taccagaaac gtaacgaaac caacgaactg tttattccac
    30301 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg
    30361 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcgatc gtcgggatca
    30421 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctccggt caggaacagc
    30481 cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcatcggtt gatcagttcc
    30541 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    30601 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    30661 gggttgatca gggtaaacgt gttggcgaac tttccggtgg tgagcgcggt cgtctgcatc
    30721 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    30781 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    30841 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    30901 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaaa
    30961 aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag cgtattgcga
    31021 agtaatgcgt aaaatgccgg atgcggcgcg aacgccttat ccggcctaca aaaccagcaa
    31081 tttcaatacg ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc atcaggcatt
    31141 gagcaccgaa tgccggatgc ggcgtgaaca ccttttccgg tctacaacag ccttagcatc
    31201 ctcaatttcg tggcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    31261 ctcttccaga gaggttttca actcatcaaa aaaactcatt ttatacctcg cctttgagca
    31321 gtttcgtcac ggttatcctt gctcccccat catctcccgc accagctcca cgcagcgcag
    31381 gaaacggctg tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    31441 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    31501 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    31561 atcaatcagc gcctgcacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    31621 ggtagtgaca aaatcggtgt caataaacgc cactttattg gcgtatttca ctgcaaaatc
    31681 aatgtattgt gcgtggccca acgcgatttt atcgtagtcg gaatactgca atgcgatctc
    31741 atcaccgccg aggtgtgaaa agacataatc gcggccatat tcccacgcac tggtggtgtt
    31801 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc cgccaaggat
    31861 cgccacggta cgcacaaaaa acggcttcac ttcggtagga atatattccc agtagcggaa
    31921 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    31981 caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    32041 gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    32101 atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tacggatatt
    32161 tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg tgggctgctg
    32221 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcggata tcgtcaaaac ccataatgat
    32281 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    32341 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa attcaagccc
    32401 caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    32461 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    32521 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    32581 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    32641 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    32701 ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg gatctcccct
    32761 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    32821 gaaaccatcg ccagcagtaa cgctaagtcg gcgctggttt cggttacctt cacgccgccg
    32881 accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    32941 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    33001 attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    33061 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    33121 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    33181 gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc atcgccatcc
    33241 agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag cgaaccatct
    33301 ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa acgcgtcaga
    33361 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    33421 tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc ggcaatcagg
    33481 cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    33541 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc
    33601 atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc cgcaccaggg
    33661 ttaccgccaa tcagaatggc acttcctggc accacgccgc cgcctagtac gcggtcgaac
    33721 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    33781 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    33841 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    33901 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    33961 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc aggcaattac
    34021 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    34081 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    34141 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    34201 atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc gtctacgatg
    34261 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    34321 gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca
    34381 atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa gcctggcatc
    34441 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    34501 acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttacc
    34561 tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca ttcaatctgg
    34621 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    34681 cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg tgcggtgagt
    34741 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    34801 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    34861 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    34921 gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    34981 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    35041 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    35101 caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt gccttgttag
    35161 tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac
    35221 agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga
    35281 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    35341 aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg
    35401 gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca ggcggctatt
    35461 ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    35521 tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    35581 ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc
    35641 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    35701 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    35761 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    35821 tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt ctctggcgca
    35881 atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    35941 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    36001 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacta
    36061 tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc gaagcgtccg
    36121 gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca ggctcggcct
    36181 atcgcgaaac caaagatcgc ggcttccacc actgcacctt gctactcaat gccgacctca
    36241 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattactt
    36301 cagtgcgttt ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc catgagcagg
    36361 tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa
    36421 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    36481 gtagctgaga gtggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    36541 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    36601 aggtatttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    36661 gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    36721 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    36781 tcgctgatta aaatatccaa ggaaggaaat atgatcgatc tggaaaacca ggaacgggag
    36841 attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    36901 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaacag
    36961 atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga aatttatggt
    37021 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    37081 tcgtgaatag atacttgaat gtattttatt ttctatccat atgaaaaata atagtaaatc
    37141 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc cccactccgg
    37201 cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    37261 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    37321 gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc gcgccaaact
    37381 ctgcagccac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    37441 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    37501 ccacgcgagc atcaacgccc agtgctttag ctgcatctac tgcgttacgc accatgtcaa
    37561 agtcagcgat agcggcaaag tcatggtctt taaaacggat gcggttaact ttggaatcgg
    37621 tgcaggcccc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    37681 aggaaccaac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    37741 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    37801 taccagtgaa gcccagcata ccgcgaacgt tgttcacttc acgagcatct tcaaggaaag
    37861 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    37921 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atcctttttg tgacataaca
    37981 aaggcggagt caacgctccg cccaaatcca tcagaacatg gctttgccat attccatatc
    38041 agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt cacggtgacc
    38101 cagtgagccc ggttttactt tcgggccata caccagtacc ggaatgtgtt cacgcgtgtg
    38161 gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg gtgaggatca ggatgtcgtc
    38221 atcgcgcagc agagacatca gctccggcag acggcggtcg aacagttcca gacccgcagc
    38281 ataaccggcg acgtcgcgac ggtggcccca ggaagagtcg aagtcaacga agttggtgaa
    38341 gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa acagcgcgtc
    38401 caagccagtc gctttcactt ttttggtgat accgcagttg gcgtagatgt ctgcaatttt
    38461 accgacagaa accacttggc cgtgtttttc atcaaccagt ttctgcagca cggtcggtgc
    38521 tggcggctca acagccaggt cgtgacggtt accggtacgc tggaagttac cagctttgtc
    38581 accgataaac ggacgagcga taacacgacc gatattgtag ccgccgttgg tcagctcttc
    38641 acgggcgatt tcgcacagtt cgtagagttt atccagaccg aaagtttctt catggcaggc
    38701 aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt tcatgtgctc
    38761 ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga ggtaacccgg
    38821 cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt tttcgtgatc
    38881 ggagaaatat ccccactcaa acagaaccgg gacaccggcg atttcccagt gaccagacgg
    38941 ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa cttcagcgtt
    39001 gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg ccagccccag
    39061 acgggtcaga tttggcagat tgagcggccc tttacgacca ttatcagctt cgcctttggc
    39121 acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc gttctgcatc
    39181 ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt tcatatgttc
    39241 tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt cgctgatacg
    39301 gcgatagaca gttggtgtgc tttccggtgc tttatcggca agtttaattg ccgctttcac
    39361 cgctttcgcc gcttcctgcc agctgttttc gtctttcgct aggataaccg ccagcggacg
    39421 ctgaccgtct acctggtcgc ccagacgcgc catatcagta aagccgacgc tgtaatcgat
    39481 ggtgtcagat gcctgacgac gaccgccgcc cattgcaacc actgccatcc ccagcgcgcg
    39541 ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg tcagcatcgc
    39601 tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt gtgccgctac
    39661 catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca atttcgcgcg
    39721 cgcttcggcg tcatctttcg ccagtttgcc ggagatcagc atctccacgc acagcgccat
    39781 cgtgacatca aacagacgcg ggttacgata ctcacccgtc aggaactgca ccgcttcacg
    39841 aacttcaacc gcgttacctg cactggaggc cagtacctga ttcatgtcgg tgagcagcgc
    39901 ggtggtgcgc acgccagcgc cgttagccac gccaacaatc gcttcggcaa gggcttcaga
    39961 gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca ccagcgcgtc
    40021 cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga tggagtccac
    40081 ggttgcggta atatcacggg tcgcgtagaa acgtttatca gccggagcca gtgaactggt
    40141 ctgaccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt tgtcatccgg
    40201 gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag tatgaccgag
    40261 gccgcgaccg gagatcatcg gaatatagcc gccgcaggct gcgaccatcg gccccaacat
    40321 cagcgaagtg acatcgccga cgccgccggt cgagtgttta tcaacaatcg ggccattcag
    40381 atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg tcagcgagac
    40441 acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg caatctgccc
    40501 ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt catcgctcag
    40561 cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg taacctcccg
    40621 aggaaaagag tatgaggcgg cttttgccgg atgcgtctac gccttatccg gcctacgggt
    40681 agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc ttacttagta
    40741 gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc agcaggcttg ccagcaggct
    40801 ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca gttcatctgc
    40861 gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg gtttgaaacc
    40921 aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgag cgctttccgg
    40981 cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac ccgctttgat
    41041 ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg tttcgatgat
    41101 cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca ccaggtcaaa
    41161 accaacctgc tcgttacccg ccatcagcgc gcggtacggg aacaccacgt caacttcatc
    41221 agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt cgtcgttacc
    41281 gtgtgggaag ttggttaccg tagcgatacg gatttccggg gtgccctgct ctttcagagt
    41341 tttgcgagca atcgggataa aacgaggata gatacagata gcggcggtat tgccgaccgg
    41401 agttttggcc tgatgacaca gagcaattac tttctcgttg gtgtcgtcgt cattcagggt
    41461 ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag tcatttcatt
    41521 ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct aacatctact
    41581 ccgcaacaca cttcgataca catcacaatt aaggaaatct acttacaagt ttgcatcact
    41641 gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc
    41701 atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct tcggcataaa
    41761 aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc cagcaaattg
    41821 tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggcactg gaagcagaaa
    41881 acaatctgcc ttacccacag cttcctgccg aagcccgccg cgcgctggat gaaggtgtaa
    41941 tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta cccgattacg
    42001 ctcgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa gatcttgatg
    42061 acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca tcgatgccgg
    42121 tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta acacaaaacg
    42181 agatcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg ccagatgcct
    42241 ttatgcacgc caatatcgtc ccgtctgatt cgcccattac ccgcgcgatc ttacgtgcag
    42301 acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc tgaaatcacc cctgatgacc
    42361 tgctgctgga agtggcgaag aacatctgtg aatgtagcaa accgcacatc gccaacggtc
    42421 cggtgcatga taaaattttc acaaaagggg gctacgggat tgtgagctgt tacaactcac
    42481 tgccgctggc gggtggtggc agcacgctgg tacgccttaa cctgaaagcc attgccgagc
    42541 gcagcgaatc gctggatgac ttctttacgc gcactctacc gcactactgc cagcagcaga
    42601 tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca atcacacttc tttgagaata
    42661 gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt tgtgccaatg tttggcatgt
    42721 atgggctggc ggaagcggtt aacttgctgt gtgagaaaga agggattgcc gcgcgctacg
    42781 gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag cgcgcaactg gcggagtttg
    42841 tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc catgttacac gcacagtcgg
    42901 ggatcagttc cgatatcggc accacgccgg gcgcgcgttt gccgtatggc gatgagccag
    42961 atccgatcac ccatctgcaa actgtcgccc cgcatcatgc ttattattat tccggcatca
    43021 gcgacattct gacgctcgac gaaatcatca aacgtaaccc gcaggcactg gtacagcttt
    43081 gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc caatgtcagc ggtaacgatc
    43141 tggttcgcgt taccggttat atggtgcgtt tgtcggattt agaaaaatat cgcgccgaag
    43201 gttcacgcac caacaccacc tggctgggcg aagaagccgc acgcaacact cgtattctgg
    43261 aacgccagcc gcgcgtgata agccatgaac agcagatgcg ctttagtcag taagattatc
    43321 cccttctcct gcgttgacgg gccaggcagt cgtctggctc tgtttttgca gggctgcaat
    43381 ctgcgctgca aaaactgtca caatccgtgg acgatgggac gttgcaatgg ctgtggggag
    43441 tgcgtgccac agtgtccgca tcaggcgttg cagattgttg acggcaaagt ggtgtggaac
    43501 gctgtggtct gcgagcaatg tgatacctgc ctgaagatat gtccgcaaca tgccacacca
    43561 atggcgcaat ccatgagcgt ggacgaagtg cttagccatg tgcgcaaagc ggtgctgttt
    43621 attgaaggga taacggtaag cggcggcgaa gccacgaccc aactgccgtt tgtagtggcg
    43681 ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc tcacctgtct ggtggacagt
    43741 aacggtatgt tgagcgaaac cggctgggaa aaattactcc cggtgtgtga cggcgcaatg
    43801 ctcgatctca aagcatgggg tagcgaatgt catcaacaat taaccggacg cgataatcaa
    43861 cagattaagc gcagcatcta tttgctggca gagcgcggca agctggcgga actgcgtttg
    43921 ctgatgatcc caggccaggt ggattatttg caacacatcg aagaactggc ggcgtttatc
    43981 aagggacttg gcgatgttcc ggttcgcctg aacgcgtttc atgcccacgg cgtgtatggc
    44041 gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg agccgttggc tgatgcatta
    44101 aaggtgcgcg gggtgagccg gttgatattt ccggcgctct atttgtgaca tgccggatac
    44161 ggcctacggt tcggcacaga cttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    44221 attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg ttcggcacaa
    44281 acctgtaggc ctgataagac gcgttaagcg tcgcatcagg cattatgcgc cgactgccgg
    44341 atgctgccta cgaagggcaa actacccacg aacgttaaac accgcatacg tgttattaag
    44401 caacacttcg gcaatctcat ccgcgggttc cgggcgtaac tcgcaaagca cggcaaacac
    44461 acgggcagcc tgttccgggc ggttaggctg cccctgaaaa ccgttgagcg gcatatccgg
    44521 cgcatcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat cgcgggtttt
    44581 actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca gctgtacaaa
    44641 ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca acaccagcac gcggcaaatc
    44701 gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc cgtgaatgca ggatcaccgg
    44761 cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt aaccactgct gcctctcaaa
    44821 ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca ccttcgccgg
    44881 acgcctttcc agcgcctgct gtagctgctc aagagacaca tcgctatgtt tttccaacat
    44941 accaggatgc aagcccaatg cggcatacag cggttgataa ttttccgcta acgcctgcac
    45001 gcgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc ccgcttgtgc
    45061 cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc gggaaatcaa aatggcagtg
    45121 agtgtcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg gtcgttcgcc
    45181 tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc aggcggaatg
    45241 acaatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc tttttcagtt
    45301 aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag tttatagtct
    45361 tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt cggcggataa
    45421 atttcgaata tccgcagctt gcccggtggt ttctcaataa attgctgaat gtcacgatag
    45481 ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc acccagccag
    45541 cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac agtgcggatg
    45601 acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc atcactgatc
    45661 ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaaa gccaggtatc
    45721 gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa gtaattcggc
    45781 gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc aaacaaccgc
    45841 gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag ccagtcgaga
    45901 tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg ttttgtggta
    45961 tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc cgagaggttc
    46021 tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc gcgcataaac
    46081 tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca caccagagct
    46141 attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag cgttacaggt
    46201 attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa agaaagacaa aataacgcaa
    46261 tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga taaaattttc
    46321 ttaatggtct aaaatctagg gtcgttttcg gcccatgaac aaactcacca ggaacagaat
    46381 aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca gaccaccaaa
    46441 cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac gaaacataag
    46501 cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc actcaatgcg
    46561 atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac gaattacgac
    46621 gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
    46681 actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct gaaccacgcc
    46741 atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca gcagtttggc
    46801 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc cttctttagc
    46861 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt tcgccacttt
    46921 cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca cgactttttg
    46981 atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca gcgccgcctt
    47041 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
    47101 gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt aggcagagcc
    47161 ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct tcagtcttgt
    47221 catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctggca acacgaggtt
    47281 gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc catcacaaaa
    47341 gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat atcggtagaa
    47401 tgacaatcga cggctttaga tactgatatc tacgcattga acggtattta acgccatcag
    47461 aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg atatcagcga
    47521 aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata aggaagggaa
    47581 attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc acgggtctgg
    47641 tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat gctggtagcg
    47701 atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt acgcttgaac
    47761 tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac gttgactgac
    47821 tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac atcaaactgc
    47881 agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa cacctgcacc
    47941 gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt cagcggatct
    48001 ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt gaacttcatc
    48061 atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg caggccgagg
    48121 atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa ggtcagcgcg
    48181 tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc tttttcatat
    48241 ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcgggtc catgttggcc
    48301 tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt acgggtatcg
    48361 gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc catccagcat atgatcaacg
    48421 ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc aaggaacagc
    48481 tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg cagctgctgt
    48541 gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt aacaatgcgg
    48601 acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc tttataaagg
    48661 tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aggtgatcgg tgcgcagcca
    48721 attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat atcacggtca
    48781 agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc catcagctta
    48841 cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca gtccaccgcc
    48901 gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta
    48961 accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat cccacgctgc
    49021 ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc ttttactgta
    49081 ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt agttttaccg
    49141 gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttg caaataagga
    49201 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
    49261 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
    49321 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
    49381 cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
    49441 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
    49501 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
    49561 agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg caaggcgcta
    49621 aaaccgttgg tgatgatgcc gattttagcg ttgccacgaa tggcgttaag cagagaaacc
    49681 gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc gttaagcaag
    49741 cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat
    49801 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
    49861 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
    49921 aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
    49981 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
    50041 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
    50101 gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt
    50161 tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga cgctgatagt
    50221 cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
    50281 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
    50341 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
    50401 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
    50461 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
    50521 gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
    50581 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
    50641 aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc gtttgccacc
    50701 atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa cgcgccaggg
    50761 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
    50821 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
    50881 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
    50941 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
    51001 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
    51061 cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac gcccgtttta
    51121 gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac tactggccga
    51181 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccagtgggga
    51241 cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag cagatgctgg
    51301 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc
    51361 gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac agcgtcgatg
    51421 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
    51481 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
    51541 gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
    51601 gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc aacggtgttg
    51661 aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggtcgtttt gatatgatca
    51721 tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
    51781 ttcgcggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
    51841 tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt atcgcgcaaa
    51901 ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa ggttgagtat
    51961 ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc cgacctacaa
    52021 aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca tctggcatct
    52081 gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt tccagcaatt
    52141 acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg
    52201 tggtagcaat tctttttaag aattgatgg
//
LOCUS       DADKXM010000013        51464 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.013, whole genome shotgun sequence.
ACCESSION   DADKXM010000013 DADKXM010000000
VERSION     DADKXM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.013"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..399
                     /locus_tag="JQR05_000774"
     CDS             <1..399
                     /locus_tag="JQR05_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HAY5323003.1"
                     /translation="DVIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERT
                     MATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLA
                     RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            414..1340
                     /gene="pdeC"
                     /locus_tag="JQR05_000775"
                     /pseudo
     CDS             414..1340
                     /gene="pdeC"
                     /locus_tag="JQR05_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            complement(1343..1666)
                     /gene="soxS"
                     /locus_tag="JQR05_000776"
     CDS             complement(1343..1666)
                     /gene="soxS"
                     /locus_tag="JQR05_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY5323004.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1752..2216
                     /gene="soxR"
                     /locus_tag="JQR05_000777"
     CDS             1752..2216
                     /gene="soxR"
                     /locus_tag="JQR05_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY5323005.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2922..4271
                     /locus_tag="JQR05_000778"
     CDS             2922..4271
                     /locus_tag="JQR05_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5323006.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTVFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFSMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4422..6071
                     /locus_tag="JQR05_000779"
     CDS             4422..6071
                     /locus_tag="JQR05_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY5323007.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6225..6968)
                     /locus_tag="JQR05_000780"
     CDS             complement(6225..6968)
                     /locus_tag="JQR05_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY5323008.1"
                     /translation="MYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGTLNACSAMHV
                     QFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFKADLSFSKLDLINFE
                     HADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCH
                     FNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDC
                     TSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(6978..7517)
                     /locus_tag="JQR05_000781"
     CDS             complement(6978..7517)
                     /locus_tag="JQR05_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323009.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIF"
     gene            complement(7695..8834)
                     /gene="actP"
                     /locus_tag="JQR05_000782"
     CDS             complement(7695..8834)
                     /gene="actP"
                     /locus_tag="JQR05_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY5323010.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALSLGLGLM
                     FGTAGLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYK
                     DAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLY
                     ANVFKKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPI
                     ILLSMYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSI
                     SVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(8831..9145)
                     /locus_tag="JQR05_000783"
     CDS             complement(8831..9145)
                     /locus_tag="JQR05_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY5323011.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9345..11303)
                     /gene="acs"
                     /locus_tag="JQR05_000784"
     CDS             complement(9345..11303)
                     /gene="acs"
                     /locus_tag="JQR05_000784"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY5323012.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPSVVEKLLEEKQAIAMPS"
     gene            11695..13131
                     /gene="nrfA"
                     /locus_tag="JQR05_000785"
     CDS             11695..13131
                     /gene="nrfA"
                     /locus_tag="JQR05_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY5323013.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13176..13742
                     /gene="nrfB"
                     /locus_tag="JQR05_000786"
     CDS             13176..13742
                     /gene="nrfB"
                     /locus_tag="JQR05_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY5323014.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            13739..14410
                     /gene="nrfC"
                     /locus_tag="JQR05_000787"
     CDS             13739..14410
                     /gene="nrfC"
                     /locus_tag="JQR05_000787"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY5323015.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14407..15363
                     /gene="nrfD"
                     /locus_tag="JQR05_000788"
     CDS             14407..15363
                     /gene="nrfD"
                     /locus_tag="JQR05_000788"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY5323016.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15383..17101
                     /locus_tag="JQR05_000789"
     CDS             15383..17101
                     /locus_tag="JQR05_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY5323017.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17094..17477
                     /gene="nrfF"
                     /locus_tag="JQR05_000790"
     CDS             17094..17477
                     /gene="nrfF"
                     /locus_tag="JQR05_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY5323018.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17474..18070
                     /gene="nrfG"
                     /locus_tag="JQR05_000791"
     CDS             17474..18070
                     /gene="nrfG"
                     /locus_tag="JQR05_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY5323019.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18412..19725
                     /gene="gltP"
                     /locus_tag="JQR05_000792"
     CDS             18412..19725
                     /gene="gltP"
                     /locus_tag="JQR05_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY5323020.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(19803..20492)
                     /locus_tag="JQR05_000793"
     CDS             complement(19803..20492)
                     /locus_tag="JQR05_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5323021.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20586..22733)
                     /gene="fdhF"
                     /locus_tag="JQR05_000794"
                     /pseudo
     CDS             complement(20586..22733)
                     /gene="fdhF"
                     /locus_tag="JQR05_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(22931..24397)
                     /gene="mdtP"
                     /locus_tag="JQR05_000795"
     CDS             complement(22931..24397)
                     /gene="mdtP"
                     /locus_tag="JQR05_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY5323022.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSTNGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLHALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTHLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24394..26444)
                     /gene="mdtO"
                     /locus_tag="JQR05_000796"
                     /pseudo
     CDS             complement(24394..26444)
                     /gene="mdtO"
                     /locus_tag="JQR05_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26444..27466)
                     /gene="mdtN"
                     /locus_tag="JQR05_000797"
     CDS             complement(26444..27466)
                     /gene="mdtN"
                     /locus_tag="JQR05_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY5323023.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIIS
                     LKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEG
                     KVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIIVDKPDPEMFRIGASAV
                     ANLEPQ"
     gene            complement(27485..27760)
                     /locus_tag="JQR05_000798"
     CDS             complement(27485..27760)
                     /locus_tag="JQR05_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323024.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(27969..29954)
                     /gene="yjcS"
                     /locus_tag="JQR05_000799"
                     /pseudo
     CDS             complement(27969..29954)
                     /gene="yjcS"
                     /locus_tag="JQR05_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
     gene            30245..30952
                     /locus_tag="JQR05_000800"
     CDS             30245..30952
                     /locus_tag="JQR05_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5323025.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVSHTGQEAISRIEG
                     EPDIALVVLDMMLPDTNGLRVLQQIQKLRPALPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRWVGALAVNEEKNGGLSFNGWYLDTIRCQLHSPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            complement(30949..31950)
                     /locus_tag="JQR05_000801"
     CDS             complement(30949..31950)
                     /locus_tag="JQR05_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY5323026.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLNMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(31947..32807)
                     /locus_tag="JQR05_000802"
     CDS             complement(31947..32807)
                     /locus_tag="JQR05_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY5323027.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            complement(32822..33505)
                     /locus_tag="JQR05_000803"
     CDS             complement(32822..33505)
                     /locus_tag="JQR05_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY5323028.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            complement(33560..34501)
                     /locus_tag="JQR05_000804"
     CDS             complement(33560..34501)
                     /locus_tag="JQR05_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY5323029.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(34532..35506)
                     /locus_tag="JQR05_000805"
     CDS             complement(34532..35506)
                     /locus_tag="JQR05_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY5323030.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            complement(35503..37023)
                     /locus_tag="JQR05_000806"
     CDS             complement(35503..37023)
                     /locus_tag="JQR05_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY5323031.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LIISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            37137..39401
                     /locus_tag="JQR05_000807"
     CDS             37137..39401
                     /locus_tag="JQR05_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY5323032.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDNILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LADFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     EQSYPDLRSGERLIMIFALLAVVITAFAGWYIYRNLGSNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNTNSPDAPRIQRAQQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLTIEIEAQSPAWSAWIDVSQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQTAVNGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQVLGQIEG"
     gene            39482..40114
                     /locus_tag="JQR05_000808"
     CDS             39482..40114
                     /locus_tag="JQR05_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5323033.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            40183..40512
                     /locus_tag="JQR05_000809"
     CDS             40183..40512
                     /locus_tag="JQR05_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323034.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(40659..41417)
                     /gene="phnP"
                     /locus_tag="JQR05_000810"
     CDS             complement(40659..41417)
                     /gene="phnP"
                     /locus_tag="JQR05_000810"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY5323035.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILSHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(41419..41853)
                     /gene="phnO"
                     /locus_tag="JQR05_000811"
     CDS             complement(41419..41853)
                     /gene="phnO"
                     /locus_tag="JQR05_000811"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY5323036.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(41840..42397)
                     /gene="phnN"
                     /locus_tag="JQR05_000812"
     CDS             complement(41840..42397)
                     /gene="phnN"
                     /locus_tag="JQR05_000812"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY5323037.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEISLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(42397..43485)
                     /gene="phnM"
                     /locus_tag="JQR05_000813"
     CDS             complement(42397..43485)
                     /gene="phnM"
                     /locus_tag="JQR05_000813"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY5323038.1"
                     /translation="MTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGKTTLLKSISAR
                     LTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSAGGNIGERLMA
                     TGARHYGDIRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANRE
                     KYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATH
                     AHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQL
                     GLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQNPAQALNLQDRGVIGEG
                     KRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(43512..44357)
                     /gene="phnJ"
                     /locus_tag="JQR05_000814"
     CDS             complement(43512..44357)
                     /gene="phnJ"
                     /locus_tag="JQR05_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY5323039.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(44350..45408)
                     /gene="phnI"
                     /locus_tag="JQR05_000815"
     CDS             complement(44350..45408)
                     /gene="phnI"
                     /locus_tag="JQR05_000815"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY5323040.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(45408..45992)
                     /gene="phnH"
                     /locus_tag="JQR05_000816"
     CDS             complement(45408..45992)
                     /gene="phnH"
                     /locus_tag="JQR05_000816"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY5323041.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLKALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(45989..46441)
                     /gene="phnG"
                     /locus_tag="JQR05_000817"
     CDS             complement(45989..46441)
                     /gene="phnG"
                     /locus_tag="JQR05_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY5323042.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(46442..47167)
                     /gene="phnF"
                     /locus_tag="JQR05_000818"
     CDS             complement(46442..47167)
                     /gene="phnF"
                     /locus_tag="JQR05_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY5323043.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(47188..47967)
                     /gene="phnE"
                     /locus_tag="JQR05_000819"
     CDS             complement(47188..47967)
                     /gene="phnE"
                     /locus_tag="JQR05_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY5323044.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPLAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(48073..49089)
                     /gene="phnD"
                     /locus_tag="JQR05_000820"
     CDS             complement(48073..49089)
                     /gene="phnD"
                     /locus_tag="JQR05_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5323045.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(49114..49902)
                     /gene="phnC"
                     /locus_tag="JQR05_000821"
     CDS             complement(49114..49902)
                     /gene="phnC"
                     /locus_tag="JQR05_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY5323046.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALPGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(50035..50478)
                     /gene="yjdN"
                     /locus_tag="JQR05_000822"
     CDS             complement(50035..50478)
                     /gene="yjdN"
                     /locus_tag="JQR05_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY5323047.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(50736..51071)
                     /locus_tag="JQR05_000823"
     CDS             complement(50736..51071)
                     /locus_tag="JQR05_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY5323048.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT        11354 a        13533 c        14283 g        12294 t
ORIGIN      
        1 gacgtcatcg tctgcgcgga aatggacgaa cagtggggat acgtcggggc taaatcgcgc
       61 cagcgctggc tgttttacgc gtatgacagg ctccggaaga cggttgttgc gcacgtattc
      121 ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg
      181 gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac
      241 gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac
      301 ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa
      361 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccaacgcc
      421 caattgcagc cattgttgcg acttcatatc aacgtcttat aacccatttt tataatcatc
      481 ttatttttgc gttgcccgcc ggtattttgg ggagtcttgt tctgctatta ctctggctac
      541 gtattcgaca aaactattta tctcccaaac gtaaattgca acgcgccctc gaaaaacatc
      601 aactttgtct ttattaccag ccaataatcg atatcaaaac agaaaaatgt atcggcgctg
      661 aagcgttgtt acgttggcct ggtgagcagg ggcaaataat gaatccggca gagtttattc
      721 cgctggcaga aaaggagggg atgatagaac agataactga ttatgttatt gataatgtct
      781 tccgcgatct gggcgcgtac ctggcaacac atgccgatcg ctatgtttct attaacctgt
      841 cggcctccga ttttcatacg tcacggttga tagcgcgaat caatcagaaa acagagcaat
      901 acgcggtgcg tccacagcaa attaaatttg aagtgactga gcatgcattt cttgatgtcg
      961 aaaaaatgac gccgattatt ctggctttcc gccaggctgg ttacgaagtg gcaattgatg
     1021 attttggtat tggctactct aacttgcata accttaaatc attgaatgtc gatattttga
     1081 aaatcgataa atcgtttgtt gaaacgctga ccacccacaa aaccagtcat ttgattgcgg
     1141 aacacatcat cgagctggcg cacagcctgg ggttaaaaac gatcgcagaa ggcgtcgaaa
     1201 ctgaggagca ggttaactgg ctgcgcaaac gcggcgtgcg ctattgccag ggatggtttt
     1261 ttgcgaaggc gatgccgccg caggtgttta tgcaatggat ggagcaatta cccgcgcggg
     1321 agttaacgcg cgggcaataa aactacaggc ggtggcgata atcgctggga gtgcgatcaa
     1381 actgccgacg gaaaacgcgg gagaaggtct gctgcgagac ataacccagg tccattgcga
     1441 tatcaaaaat cggacgctcg gtggtgcgca actcaacggc ggccagtaac aggcggcgtt
     1501 ggcgaatgta atcgccaagc gtctgatgcg tcaccgtgcg gaacatccgt tgcaagtacc
     1561 actttgaata gcctgatttt tttgcgacga catcaatgtt aagcggctgg tcaatatgca
     1621 cgtcaatcca tgcgataaga tcctgaataa ttttctgatg ggacataaat ctgcctcttt
     1681 tcagtgttca gttcgttaat tcatctgttg gggagtataa ttcctcaagt taacttgagg
     1741 taaagcgatt tatggaaaag aaattacccc gcattaaagc gctgctaacc cctggcgaag
     1801 tggcgaaacg cagcggtgtg gcggtatcgg cgctgcattt ctatgaaagc aaagggttga
     1861 ttactagtat ccgtaacagc ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg
     1921 tcgcaattat caaaatcgct cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt
     1981 ttggcgtgtt gccagaaggg catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc
     2041 aatggcgaga agagttggat cggcgcattc ataccttagt ggcgctgcgt gacgaactgg
     2101 acggatgtat tggttgtggc tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg
     2161 accgcttagg agaagaaggt accggcgcac gcttgctgga agatgaacaa aactaaagcg
     2221 ccacaaggac aagctgactc atcgttaact atggggccgg ggtgagggta aatatttgca
     2281 ctggtgctgc ctgttcccct caccctaacc ctctccccga aggggcgagg ggactgatcg
     2341 agctcttttg gaattatccg caaacacaaa actaaagcgc cacaagggcg ctttagtttg
     2401 ttttccggtc tttgtctttc tctctatccc gctggaacac aggaggggtt cccccgacgt
     2461 caacacacct cattcgagca cgtggtggag gttccggttg gtgttgatgc tttaattgta
     2521 tgtcaccgac gtttcttcgc cagtgtaaaa gtatactttt tagccgcaat atttttgtca
     2581 tctcagacga ttttttatcg caatccagaa cggtatacgg ctcgataacg ctgcaatctt
     2641 gcgcatcgac gataacgttt gcgcatcaat tgcctggttt ttcatcgtca agacaataaa
     2701 agagaaaaaa gcagcaaact tcggttgaaa aagccgctat gatcgccgga taatcgtttg
     2761 ctttttttta ccacccgttt tgtatgcgcg gagctaaacg tttgcttttt tgcgacgcac
     2821 caaattgccg caaacctgga gcaggaagat aacgtttcgc tggcagggga ttgtccgcca
     2881 cgcatcttga cgaaaattaa actctcaggg gatgttttct tatgtctacg ccatcagcgc
     2941 gtaccggcgg ttcactcgac gcctggttta aaatttcaca acgtggaagc actgtccgtc
     3001 aggaagtggt tgccgggtta acaacgtttc tggcgatggt ctactcggtc atcgtcgttc
     3061 caggtatgtt gggtaaagca ggcttcccgc ctgcggcagt tttcgttgca acctgtctgg
     3121 ttgccggact cggttctatc gtgatgggtc tgtgggctaa tctgccgttg gcgattggtt
     3181 gcgccatctc cctgacggtg tttaccgcat tcagcctggt gctggggcaa catattagcg
     3241 tacctgtcgc gctgggtgcc gtgttcctga tgggtgtgct gtttacggta atttctgcca
     3301 cgggtatccg tagctggatt ttgcgcaact tgccgcacgg tgtggcgcac ggcacgggga
     3361 tcggtatcgg tctgttcctg ctgctcattg ccgctaatgg tgtcggtctg gtgattaaaa
     3421 acccgcttga tggtctgccc gttgcgctgg gtgatttcgc gaccttcccg gtgattatgt
     3481 cactggtagg tctggcggtg atcatcggct tggaaaaact gaaagtccct ggtggcattc
     3541 tgctgaccat tatcggtatc tcaattgtcg gtttgatctt cgatcctaac gtccatttct
     3601 ccggcgtttt ctccatgcct tcattgagcg atgaaaacgg caattcactg attggcagcc
     3661 tggacattat gggcgcgctg aatcctgtag tcctgccaag cgttctggcg ctggtgatga
     3721 cggcagtatt tgatgccacc ggaactatcc gcgctgtcgc cggccaggcg aacctgctgg
     3781 ataaagatgg gcagatcatc gacggcggga aagcactgac cactgactcc atgagcagcg
     3841 ttttctctgg cctggtgggt gcagctccgg cagcggtata catcgagtct gcggcgggta
     3901 cggcggcggg cggtaaaacc gggttgacgg ctatcaccgt tggcgtgctg ttcctgctga
     3961 ttctgttcct ctctccgctt tcttacctcg ttccggggta tgcaacggct ccggcgctga
     4021 tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat cgactttgct gattttgttg
     4081 atgcgatggc gggtctggtt acggcggtat tcatcgtgct gacctgtaac atcgtaacag
     4141 gcatcatgat cggcttcgcg actctggtga ttggtcgtct ggtttccggt gaatggcgca
     4201 agttgaacat cggtacggtc gttatcgccg tggcgctggt gaccttctat gcgggtggct
     4261 gggctatcta atctttcatc ggattctgaa aacgggtggc aatggctgcc cgtttttatt
     4321 ttctccgcac attgtgtgag cttttgcgat attctgaaaa aatgagaatt caggcataac
     4381 gtctttctca gatcataaaa aacatcgcaa acagggaacg catggaaatc ttcttcacca
     4441 tactgataat gaccctcgtg gtctcgctgt ccggggtggt cactcgtgtc atgccctttc
     4501 agatcccgct tccgcttatg caaatcgcca tcggtgcgct actggcgtgg ccgacgtttg
     4561 gtttgcatgt ggagtttgat cctgaactct ttttagtctt gtttatcccg ccgttgctgt
     4621 tcgctgatgg ctggaaaacg ccgacccgtg aatttctcga acatggtcga gagattttcg
     4681 gcctcgcgct ggcgctggtg gtggtcaccg tggtcggcat tggcttcctt atttactggg
     4741 tggtgccggg cattccgctg atccccgcct ttgcgctggc ggcggtactt tctccgaccg
     4801 atgctgtggc gctctccggg attgttggcg aagggcgcat cccgaaaaaa atcatgggca
     4861 ttttgcaggg cgaagcgttg atgaatgacg cttccggcct ggtgtcgttg aagtttgccg
     4921 tggcagtggc gatggggacg atgatcttca ctgtcggcgg tgcgacggtc gaatttatga
     4981 aagtagccat tggcggtatt cttgccggtt ttgtggtgag ctggctgtat ggtcgctcgc
     5041 tgcgattcct cagccgctgg ggcggtgatg aacccgcgac gcagatcgtt ctgctgttct
     5101 tgctgccatt cgcttcttat ctgattgccg aacatattgg cgtttcgggc atcctcgctg
     5161 cggttgccgc cgggatgacc atcacccgct ccggtgtgat gcgccgtgcg ccactggcaa
     5221 tgcgcctgcg cgcaaacagc acctgggcga tgctggaatt tgtctttaac ggcatggtgt
     5281 tcctgctgtt aggtctgcaa ctgccgggta ttctggagac gtcgctgatg gcggcagaaa
     5341 tcgaccctaa cgtcgaaatc tggatgctgt ttaccgatat tattctgata tatgcggcgc
     5401 tgatgctggt ccgtttcggc tggctgtgga cgatgaaaaa gttcagcaac cgcttcctga
     5461 agaagaagcc gatggagttt ggttcgtgga ccacacgaga aatcctgatc gcgtctttcg
     5521 ctggggtgcg tggggcgatc actctggccg gtgtgctctc aatcccgctg ctcttgccgg
     5581 atggtaacgt cttcccggcg cgctatgagc tggtgttcct ggcggctggc gtcattctct
     5641 tctcgctgtt tgtcggcgtg gtgatgttgc ctattctgct acaacacatt gaagtcgcgg
     5701 accattcgca acaattgaaa gaagaacgta ttgcgcgagc ggcaacggca gaagtggcga
     5761 ttgtggcgat ccagaaaatg gaggagcgtc tggcggcgga taccgaagag aatatcgata
     5821 accagttgct tacggaggtc agttctcgcg tcattggtaa cctgcgtcgt cgcgccgatg
     5881 gacgtaacga cgttgaaagt tccgtgcagg aagagaacct tgagcgtcgc ttccgtctgg
     5941 cggcattgcg ttctgaacgt gctgaactgt accacctgcg cgccacgcgg gagatcagca
     6001 acgaaacgct gcaaaaatta ctgcacgatc tcgatttgct tgaagcgttg ctaattgagg
     6061 aaaatcagta agccgaaagc gacgtaacca ggcccgaggt aatctgaaaa aggtgcaatc
     6121 ttttgcacct ttaacaggtt agctcgggcg aacattcatg attctaaaac aaataagtta
     6181 tctctcttgt aaagtcaatt cattggctgt tcttcgactt aacctcaatc tgcgtgttgc
     6241 ataatatctg cagtaatctt catagcgact gcattaatat tatcctcagt ttgtgacatg
     6301 ccagtatgag aggtacaatc ttcttcaaag aatttctgta gtgttaccag ggtatcacta
     6361 tcaatgggga tattttcctc cccacggaga atggaaatgc ccattccctg gacgctagcc
     6421 tcatcgaaat taacccgata gagacgggta ttaaacatac aggcttttgt taaaatagaa
     6481 cagttgaaat ggcagttgct taaattggca ttattaaaat taacattagc catattagag
     6541 ccggtacata taaccatttc gaggaatgta tttgtcagcc acgtacaaaa gagtttgcta
     6601 tcaattaagt ttatattctg gaggattgct ttgttcagat tgacgcgaga cagatcagca
     6661 tgttcaaagt taattaaatc gagtttagag aaactaaggt cggctttaaa taaattaaca
     6721 tatggcgcta ttactttata aagatttacc tcaaccatgt acgccatata aaaatttgaa
     6781 tagtcaagga aggtattttt aatattggct ctattcattg ctgcattgta gaattgaaca
     6841 tgcatagcgg agcatgcatt tagagttccg tcggccattt ttatctgagc gaaatttgag
     6901 tcatcgagaa tacaattatc aaaaagtatt ttttccatta tggcataata aaaattcgtt
     6961 ttatacatat tacagtccta gaaaatagcg ttttgtagtc tgcaacctgc aaaattcgaa
     7021 ttggataaat cttcatcttt aatcacgata cccttcaggc taaggtgtga aaaatcgtaa
     7081 ccaccattta ctatctgtgg aatttcgata gtattattat ggacggcgac attatttata
     7141 aaatatacca ccttaatgga tacatccttt tctttactaa ggtaaactct tctcgtaatt
     7201 ctatcgtcac atttattatt tgcatataac gctatatcta taaaatattt atgtattttc
     7261 ctgtccgttt ctttcgtgat ttttcctgat ggtaaactat cataaattct tttcattttc
     7321 tgaatcaagc ctatcacttt ttcatgatca tctattttga aaagagagag ggctttattg
     7381 tcaattaacg tgtttttatg atgtttgctg taattatctg attttgtttt ctttgtatgt
     7441 gatgaacttg tgatagagtg gttatcgtta atctggcaaa gagggaaaaa catattattt
     7501 aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg catattctct
     7561 gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta
     7621 atattatggg aaaaggtgat gcatgttgga gaggaagagt attccccggt caggcgaccg
     7681 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
     7741 ataaactgcg cgcggaacag ttcacgctcg cgcgcccctt ccgctgagtt atcggttgcc
     7801 gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
     7861 gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tagtcgggcc gaggatcatc
     7921 agcaccactg cggtaatcag ccccagccag ccgcccagca tcgcgccacg ggtagtcagt
     7981 ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac
     8041 gcaaggccta ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatcgca
     8101 atcacgccga ggatcagtac ggtgattttt gatacccgca gctcttcacg ttcggtcgcg
     8161 ccttttttga agacgttagc gtacaagtca tgcgaaaccg ccgatgcgcc cgccagcgtc
     8221 agacccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa accgaggaat
     8281 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
     8341 tggcccgccg cgtctttata ttccggattc gcaccaacaa gcatgatcgc gccgaagccg
     8401 ataataaagg tcagaatata gaagtagccc ataaatccgg tggcgtagaa cacgctctta
     8461 cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc
     8521 gtaccaaaca tcagtcccag ccccagagag agcgcggaga tccccaagaa tgaggcggcg
     8581 gacatatagt ccccggcaat cgccagcccg ttctggaagc cagtgatatt gccgcctgcg
     8641 gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc agtaggtaat gccgagcgta
     8701 aacacgacga aaatcaggaa cataataatc gcctgccagt tcgttggctg gcgctctacg
     8761 gccccgctaa tagcatccgc ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc
     8821 agaactcttt tcatgatgct tgtacctcat gcaggacttc attattaaga cggtcgaatt
     8881 cgccgttcgc ccgccagatg tagataccgg tgagaacaaa ggagatcaca atcactccaa
     8941 caccaattgg aataccgcgt gtgacgctgg tgttcggatt cagcggcgtg cccagccagc
     9001 cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac tgccagcata ataatcgaca
     9061 ggatggtggc aaaccgttgc cgtttttcga ctaactccct gaaatgcgca ttgtcttcta
     9121 tccgctgata aatcgtacca ttcatcacag attctccaga ggtaatgtag ggattgtttt
     9181 aattcccgct cccttatggg agaaggttaa cgctcgggta acccttgccg aatgtaggcc
     9241 ggataaggcg tttacgccgc atccggcaat caatgcctga tgcgacgctg tcgcgtctta
     9301 tcaggcctac agaccgttac cgattcgcat ccggcaattg tgggttacga tggcatcgcg
     9361 atagcctgct tctcttcaag cagcttctcg accacgctag gatcggcaag cgtcgaggta
     9421 tcgcccaggt tgctggtatc gcccgccgca attttgcgca gaatacggcg cataattttg
     9481 ccggagcggg ttttaggcag ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg
     9541 ccaatctctt tacgcaccca gttgcggact tctgcgtaca gttctggtga cggttcctcc
     9601 ccgtgattaa gcgtgacata ggcgtagatc gcctgacctt taatattgtg cggaatacct
     9661 actacggcgg cttcggcaat cttcggatgc gccaccagcg ccgactctat ctctgccgtc
     9721 cccagacggt gaccggagac gttcagcacg tcgtccacac gcccggtgat ccagtaatag
     9781 ccatcttcat cgcgacgcgc gccgtcgccg ctgaaataca tatttttgaa ggtggaaaaa
     9841 taggtctgct caaaacgttc gtgatcgcca aacagcgtac gcgcctgacc aggccaggag
     9901 tcggtgatca ccaggctacc ttcggtagcc ccttccagcg ggttaccttc gttatcgacc
     9961 agcgccggtt gcacgccgaa gaacggacgt gttgccgaac cggctttcag ctcggtagcg
    10021 ccaggcagcg gggtgatcat gaaaccgccg gtttcggtct gccaccaggt atcgaccacc
    10081 ggacatttct cgttgccgat ttttttccag taccactccc acgcttccgg gttaattggc
    10141 tcgcccacgg aaccgagaat gcgtagcgac gaacggtcgg tgccttcaat cgctttatcg
    10201 ccttccgcca tcagcgcgcg gatcgccgtg ggtgcggtat agagaatatt gacctgatgc
    10261 ttgtccacca cctgcgccat acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc
    10321 agcgtggtcg cgccgcaggc cagcgggccg tacagcaaat aactgtgtcc ggtaacccag
    10381 cccacatcgg cggtgcacca gtagatatca cccggatgat aatcaaagac atatttaaag
    10441 gtcagcgccg cgtacaccag ataaccgccg gtagtgtgca gtacgccttt tggttttccg
    10501 gtagaaccgg aggtatagag aataaacagc ggatcttcgg cgttcatctc ttccgcctgg
    10561 tgctgatcgc tggcttgctc aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag
    10621 tcgatgtttc cgccagtacg cttcagtacc accacatgct ctacgctggt gacgttcggg
    10681 ttttttagtg cgtcatcaac gtttttcttc agcggaatac tacgcccggc gcgcacgcct
    10741 tcgtcggaag tgatcaccag tcgtgagttg gaatcaataa tgcgcccggc aacggcttcc
    10801 ggcgagaagc cgccgaaaat caccgaatgc accgcgccaa tgcgggcgca ggccagcatc
    10861 gcaaccgcgg cttccggcac catcggcata taaatcgcca ccacatcacc ttttttaatg
    10921 cccagctcga gcagggtatt ggcgaagcgg cagacgtcgc ggtgcagctc tttatagctg
    10981 atatgtttgc tctggctggc gtcgtcgcct tcccagatga tggcggtgcg atcgccgttt
    11041 tcttgcagat ggcggtcaag gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat
    11101 ttaatggaca cattaccggg ggcaaaggag gtatttttca ccttctggta cggtttgatc
    11161 cagtcgagaa tttttccctg ttcgccccag aaggtatcag gtacgttaat agattgttga
    11221 tacatcgcct cgtactgctg agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg
    11281 gtgtgtttgt gaatttggct catgcttttg ttctccttgt aggatgttaa taatatgtgg
    11341 cataagcgtt aaatgtaggg gtattggcag ttttgtttag tatttgggcg acagatcacg
    11401 caaaagtaga atggtgcaaa taaacggcag ggtaattttt gaaggtcacc aacaaaagtt
    11461 gattaattct ttgaggaaca tgcagttatg catgctgttg aaaaagagga agatactgac
    11521 taactctaaa gtggtattta catgcactta caattgatta aagacaacat tcacagtgtg
    11581 gttatttgtt acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt
    11641 gaaaaacaat aaaaaccgcc attgcaacaa tggcgcaatt cggatgaagc ccctatgaca
    11701 aggataaaaa taaacgcacg ccgtatcttc agcttattga ttcctttttt ctttttcact
    11761 tctgttcacg ctgaacaaac ggcagctccc gcaaaacctg taactgtgga agcgaagaat
    11821 gaaacctttg ccccgcagca tcacgatcaa tatctctcct ggaaagccac ctcggaacag
    11881 tcagagcgtg ttgacgccct ggcggaagat ccacggctgg tgatcctgtg ggcggggtat
    11941 cccttctcgc gcgattacaa caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt
    12001 gaaaccctgc gtaccggtgc gccgaaaaac gctgaagatg gtccgctacc gatggcgtgc
    12061 tggagttgta aaagcccgga tgtggcgcgt ctgatccaga aagacggcga agatggctac
    12121 ttccacggta aatgggcgcg cggcggcccg gaaatcgtca acaacttagg ttgtgccgac
    12181 tgccataaca ccgcctcacc agagttcgcc aaaggcaaac cggagttaac cctttcccgt
    12241 ccgtatgcgg ctcgcgcgat ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc
    12301 gaccagcaat cgatggtttg cggtcagtgc catgtggagt attacttcga cggcaaaaac
    12361 aaagcggtta aattcccgtg ggatgacggc atgaaagtcg aaaatatgga gcagtattac
    12421 gacaaaattg ccttctctga ctggactaac tccctgtcga aaacgccaat gctgaaagcg
    12481 cagcacccgg aatatgaaac ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt
    12541 atcgactgcc atatgccaaa agtgcagaac gccgaaggca aactctacac cgaccataaa
    12601 attggtaatc cgtttgataa cttcgcccag acttgtgcga actgccatac ccaggataaa
    12661 gctgccttgc aaaaagtggt cgcggaacgt aagcaatcga ttaacgacct gaaaatcaag
    12721 gttgaagatc aactggttca cgctcacttc gaagcgaaag cggcgctgga tgcaggcgcg
    12781 acggaagccg aaatgaagcc gattcaggac gatatccgtc atgcccagtg gcgttgggat
    12841 ctggcgatcg cttcccacgg tattcatatg cacgcaccgg aagaaggtct acggatgctc
    12901 ggtacggcga tggataaagc ggcggatgcg cgcaccaaac tggcgcgcct gctggcgacc
    12961 aaaggcatca cccatgaaat ccagatcccg gatatctcga ccaaagagaa agcccagcag
    13021 gccattggcc tgaacatgga acaaatcaag gccgagaagc aggacttcat caaaacggtg
    13081 atcccgcagt gggaagagca ggcacgtaaa aacggtctgt taagccaata accccgttcc
    13141 gcctcgcaag gggcggaaaa cacaatggag tgaatatgag cgtattacgt tcgttattaa
    13201 ctgctggggt gctggcgtcg ggcctgttgt ggagcctgaa cggaattacc gctacccctg
    13261 cggtgcaggc atctggtgat cgctacgaag ttacccagca gcgtaacccg gatgccgcct
    13321 gtctggactg tcataaacca gataccgaag gtatgcatgg caaacatgct tccgttatca
    13381 acccgaataa caaattgccg gtcacctgca ccaactgtca cggccagcca tcaccgcaac
    13441 accgcgaagg ggtgaaagat gtgatgcgct ttaacgagcc gatgtacaag gtcggagagc
    13501 agaacagcgt ctgtatgtcc tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc
    13561 acgatgtcca cgtaaccaaa gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag
    13621 atacgatgca gacgttaagt gacaaaggac ggattaagat ttgcgtcgat tgccacagcg
    13681 atcagcgcac caatccgaac tttaacccgg cgtctgttcc gttgcttaag gagcagccat
    13741 gacctggtct cgtcgccagt ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac
    13801 cgcagggcgc gtggtggcga aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca
    13861 tgacgagtcg ttatgcatcg gctgtaccgc ctgtatggat gcttgtcggg aagtgaacaa
    13921 ggtgccggaa ggcgtctcgc gcctgacgat tattcgtagc gagccgcagg gcgaatttcc
    13981 tgatgtgaaa tatcgcttct tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt
    14041 tgacgtctgc ccgaccggtg cgtcttttcg tgatgccgct agcgggattg tcgatgttaa
    14101 tcctgatctc tgcgtcggtt gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt
    14161 tatccatccg gtcacgaaga cggcggacaa atgcgatttc tgtcgtaaga ccaatttgca
    14221 ggccggtaag cttcccgcgt gcgttgaagc ttgcccgacg aaggcgctga cgtttggcaa
    14281 tctggacgat cctaacagtg agatttcgca actgctgcgc cagaagccta cttaccgcta
    14341 caagctggcg ctgggaacca aaccgaagct gtaccgcgta ccgtttaaat acggggaggt
    14401 gagccaatga cgcagacttc cgcatttcat tttgaatcgc tggtgtggga ctggccgatt
    14461 gccatctacc tgtttttgat tggtatttct gccggtctgg tgacgctggc cgtgctgtta
    14521 cgtcgcttct acccgcaggc gggcggtgca gacagtacgt tgctgcgcac cacgctgatt
    14581 gtcgggccgg gcgcggtgat cctcggtctg ttgatcctcg tcttccacct gacaagaccg
    14641 tggaccttct ggaagctgat gttccactac agttttacct cggtgatgtc gatgggggtg
    14701 atgctgtttc agctctacat ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat
    14761 gatttgcttg ccctgcaaca acgctggttg ccgaagctgg ggatcgtgca aaaggttctg
    14821 agcctgctga cgcccgttca tcgcggactg gaaacgctga tgctggtgtt ggcggtgctg
    14881 ctgggggctt ataccggctt tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac
    14941 ccgagcctgc cggtgctgct cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg
    15001 atcgccatgg cgatacgcca acgcagtaac ccgcattcca cggaagcgca ctttgttcac
    15061 cggatggaaa tccccgtggt atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt
    15121 ctggcgctgg gcgatgacgg taaagttcgt gcgctggtgg cggcattagg tggcggtttc
    15181 tggacgtggt ggttctggct tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc
    15241 aaaccgtggg tcaatcgcag ttccggcatt cctgccgtgc tggcggcgtg tggggccagt
    15301 ctggtcggcg tgttgatgct gcgctttttc attctctacg ccggacagtt gacggtggcg
    15361 taagccagaa aagaggtggt ttctggacgt attccttcct gaagtcggtt ttctggcgtt
    15421 gttgttaagt ctcggggtca acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt
    15481 gcgctggcct gccatgatgc gactcacttg catcggcatt ctggcgcagt tcgcgctcct
    15541 gctgctcgcc tttggcgtac tgacgtattg ttttctcatc agcgatttct cggtcattta
    15601 tgtcgcccaa catagctaca gcctgctgtc gtgggaactc aaactggcgg cggtgtgggg
    15661 cggtcatgaa ggttcgctgc tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt
    15721 tgcctggcat tatcggcagc aaagcgatcc gctatttccg ctgacgctag ccgttttatc
    15781 tctcatgctc gccgcactgc tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat
    15841 atttccacca gcaatcgaag gccgcgatct caatccgatg ctgcaacatc ccggtcttat
    15901 ctttcatcca ccgctgcttt accttggcta tggcggtttg atggtagcgg cgagcgtggc
    15961 gctggcgagt ctactgcgcg gcgagtttga tgcggccagc gcccgaattt gctggcgctg
    16021 ggcgttacct ggctggagtg cattaacggc ggggatcatc ctcggttcct ggtgggccta
    16081 ctgcgaactc ggctggggcg gctggtggtt ctgggatccg gtggaaaacg cctctttatt
    16141 accctggctt tctgccactg cgctgctgca cagtttgtcc ctgacacgcc agcgggggat
    16201 tttccgccac tggtcgctgt tgctggcgat agttactctg atgctgtcgc tgctgggcac
    16261 cttaattgtc cgttctggca ttctggtttc ggttcatgcg ttcgcactgg ataacgtccg
    16321 cgctgtgccg ttgttcagcc tgtttgcact gattagcctt gcgtctttgg ctctgtatgg
    16381 ctggcgagcg cgggacggtg gcccggcggt gcgtttttcg gggttatcgc gggaaatgtt
    16441 aatcctcgct acgctgttgc tgttttgcgc agtgctactg atcgtgctgg tgggaacgct
    16501 ttatccgatg atttacggcc tgctgggctg gggacgcctc tccgttggcg cgccgtattt
    16561 taaccgcgcg acgttaccgt ttggtctgtt gatgctggtg gtgattgtgc tggcgacgtt
    16621 tgtctctggc aaacgcgtgc agcttccggc gctggtagct catgctggcg tgctgttatt
    16681 tgccgcgggg atcgtggttt ccagtgtcag tcgtcaggag atcagcctga atttacagcc
    16741 gggtcagcag gtgacgctgg caggatacac cttccgtttt gagcgactcg atctgcaagc
    16801 cagaggcaat tacaccagcg aaaaagcgat agtggcgctg tttgaccatc agcaacgcat
    16861 tggtgaatta acgccggagc ggcgttttta tgaagcacgc cgtcagcaaa tgatggaacc
    16921 gtcaattcgc tggaacggca tccatgactg gtatgcggtc atgggtgaaa aaaccagagc
    16981 ggatcgttac gcttttcgtt tgtatgtaca aagcggtgtg cgctggatct gggggggagg
    17041 attgttgatg attgcgggcg cattgttaag cggatggcgg gggaagaagc gcgatgaata
    17101 aagggcttct cacgctactg ctgttattta cctgttttgc gcgcgctcag gttgtagaca
    17161 cctggcaatt cgccaatccg caacaacagc aacaggcgtt aaatattgcc agccagttac
    17221 gttgtccgca gtgccagaat caaaacttac tcgaatccaa tgcgccggtg gcggtcagta
    17281 tgcgccatca ggtttatagc atggtgtcgg aggggaagaa cgaagtcgaa atcattggct
    17341 ggatgaccga acgctacgga gattttgttc gctataaccc accgttaacg ggtcagacgc
    17401 tggtgttgtg ggcgctgcca gtggtgttgt tattgctgat ggcactgatc ctctggcgag
    17461 tgagggctaa gcgatgaaac agcctcaaat accggtgaaa atgctgacaa cactcacgat
    17521 attgatggta ttcctctgtg tcggcagtta tctgttaagc ccaaaatggc aggcggtacg
    17581 tgcggagtat cagcgtcagc gcgatccgct acatcagttt gccagccagc aaaccccgga
    17641 agcgcagctt caggcattgc aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg
    17701 ggcgttactg ggcgagtact atctgtggca aaacgattac agcaattcgc tgctggcgta
    17761 tcgtcaggcg ttgcaactac gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt
    17821 gctttattac caggccagcc agcatatgac cgcccagact cgcgcaatga tcgacaaagc
    17881 cctcgcgctg gactctaacg aaatcaccgc tctgatgctg ctggcttccg atgcgtttat
    17941 gcaggcgaat tacgcgcaag ccatcgaact atggcaaaaa gtgatggatc tcaactcacc
    18001 ccggatcaac cggacacagc tggtcgagtc gattaatatg gcaaaactgt tgcagcggag
    18061 atcggattaa tcatctggtg atgaagatta tgtcattgag ctgcataaaa aataatcgaa
    18121 tgaacatatg ccaaaaataa tcaccaatca gtattattgc agattaaaca aataaaaatc
    18181 tttccataac aaatggttat tcattaatcc tgccataaac tcgcgttatt atgcattaat
    18241 gcagcgaaaa gctctgttgt taaagggttg cgcaacatac cgcgcaaatg atactgatca
    18301 taagcgttaa aaaaatctac aaaccaacgc aacacaattc atgccctggc agtatgtcac
    18361 gttctcgcgt ttctgaacgg ggaacggcgc tccattgagg aagttattct gatgaaaaat
    18421 ataaaattca gcctggcctg gcagattctg tttgctatgg tgctgggcat tctcctggga
    18481 agctacctgc actaccatag cgacagccgc gactggctgg tcgtcaattt gctctctccg
    18541 gcgggtgata tcttcatcca tctgatcaaa atgattgttg tgccgattgt gatctccacg
    18601 ctggtggtgg gtatcgcggg tgttggtgat gccaaacagc tcgggcgtat tggcgcgaaa
    18661 accattattt acttcgaggt gatcaccacc gtcgccatca ttttggggat cactctggcg
    18721 aacgtcttcc agcccggtgc cggggtggat atgtcgcagc tggcgaccgt cgatatctcg
    18781 aaatatcaga gcactacgga agcggtacaa agcagttccc acggcattat gggcacgatt
    18841 ttgtcgctgg tgccgacgaa cattgtggcg tcgatggcga aaggcgaaat gctgccgatc
    18901 atctttttct cggtgctgtt tggtctgggg ctttcttccc tgcccgcgac gcatcgtgaa
    18961 ccgctggtga ccgtgttccg ctccatctct gaaaccatgt ttaaagtgac tcacatggtg
    19021 atgcgttatg caccggtggg tgtgtttgcg ctgattgcgg tgacggtggc taactttggt
    19081 ttctcgtctc tgtggccact ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc
    19141 ttcgcgctgg tagtgctggg aattgtggca cgtctgtgcg ggttaagcgt ctggatcctg
    19201 attcgtattc tgaaagatga gctgattctg gcgtactcca ctgccagctc tgaaagcgta
    19261 ctgccgcgaa ttattgagaa gatggaagcc tacggagcac cggcgtcgat caccagcttc
    19321 gtggtgccga ccggttactc ttttaacctt gatggttcga cgctgtatca aagtattgcc
    19381 gctattttca tcgctcagct gtatggcatt gacctgtcca tctggcagga aatcattctg
    19441 gtgctgacgc tgatggtgac ctcgaaaggg attgctggcg tggctggcgt gtcgtttgtg
    19501 gtgttgctgg caacgctggg tagcgtaggt attccgctgg aaggtctggc gtttattgct
    19561 ggtgttgacc gtatcctcga catggcgcgt actgcgctga acgtggtggg taatgcgctg
    19621 gcggtgctgg tgattgccaa gtgggaacac aaatttgacc gcaaaaaggc gctggcttat
    19681 gagcgtgaag tgctgggcaa atttgataaa actgcggatc aataattgaa gattgccggg
    19741 gatatccccc ggcaatgtgt ggatgcctga tgcgacgctt gccgcgtctt atcaggccta
    19801 ccttaaccgt tggttaattt ttcaaactct tcacacccgg tatcaaaccc ttccatacag
    19861 ctcaggttca accagtgcag cgccttttgt ttgttcttct cgataaaacc ttcctcaccg
    19921 tttaagaaca tcattcccgc ccagtactcg gaataaccgg ttcgggaaat tgcagagctg
    19981 cgtttgaaat accaggttgc cttgtcatcg tcggccttaa tgcccacgcc gttggcgtaa
    20041 atcaaaccaa gcagcatttg ggcatcgacg gcggagtcat tctccagatc ttccgaggcg
    20101 ttttctaaca gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga
    20161 atatgcgcca gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga
    20221 acttttgctt gtggatagtc cagactgacc ggattggtga ttttgatctg cgccaacagc
    20281 gcgcaggcat cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc
    20341 aaatcgccgg agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc
    20401 tctgctgcct ttaaatactg gctgccaggc tcggagtcgt tcgcgtgggc aaagaatgtc
    20461 aaaaacaaca tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa
    20521 ccagtataaa gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga
    20581 cagtattacg ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc
    20641 acgtactgct cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg
    20701 tattccggcg ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggcacca
    20761 atccaccact ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca
    20821 cgggtgataa ttttgccttt acgcgagtgc actcaaacca atgcctcatc ttcaataccc
    20881 agacgtttgg cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc
    20941 agtgccgcac agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt
    21001 gacagtacca tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg
    21061 aagaactgcg ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc
    21121 tgatcggcat ctgaagtatc gcggcaaggc cactgaatga agcccagttc gcccattttc
    21181 tcgtaagtcg caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg
    21241 gtgttgttgt agtgcatcgg ataaccaata cgggtggcga tttcactgat gatttgccag
    21301 tccgttttca gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca
    21361 gccgcagtaa acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc
    21421 gccgacgcgg ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag
    21481 gctttacgta ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac
    21541 gcggcacgca cttcgccatg cgctgcgcgg tgcggcaact cgctgatgcg atagccggta
    21601 tgagcaggca ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct
    21661 ttcacgtact ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc
    21721 acgttgttct gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc
    21781 agcatcgcga ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca
    21841 cccatgcccc acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga
    21901 atctcactgg cgctgacacc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg
    21961 attttacgat actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga
    22021 ttttcttcaa taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc
    22081 agtgcaatgt gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata
    22141 attttcgccc cgttacgttt agcgttaatt acgtgattcg ccacgattgg gtgggaatcc
    22201 gccgggttgt acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg
    22261 ctcattgcgc cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca
    22321 cgagcgcagc agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca
    22381 tagttggttt cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg
    22441 tacttctctt tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atctcaggaa
    22501 acaggttcga gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc
    22561 aggatctggg tatcgttaat gaaatcccag ccataataac ccttcagaca cagggtaccc
    22621 tggttggttt tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg
    22681 ttgattttgc aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc
    22741 gctccagtta atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca
    22801 cattttattt ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa
    22861 aaacaggctg tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc
    22921 ggcagacatt ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt
    22981 tcatcaactg aatgctttgg atcacccggc ggctgtccag catcagtaat gacatctctt
    23041 cggcaagtac tggcaaccgg gcttcagtgg cctgtaagcg gctggttaag ccgcgctgat
    23101 aggcggcctc ggcagcgcgc tgggtgaagc gcgttgcttc cacgtgttca gcctgcattt
    23161 ctcgctcgtc gttgagcgtt tgcagatgcg tgccgttgac ggcaacgtca cgcaccgcgt
    23221 tcagtactga ctggttgtaa cgttcaatca tcatgttgct ggcggcgcgc gtgccttcga
    23281 gattggcatt caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga
    23341 actggcgact ggtttttttg aataaggtat ccaggtggat ggcatcaagg ccgaaaaacg
    23401 ctttgatatc aaagctcgga tagaacaacg cccgcgcgga atccacctga tctaatgacg
    23461 cctgaacata ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga
    23521 gtgtcgccgg aatgccggtc tggactcgtg gtaatgccac aggtttgatc tccggcatat
    23581 cgctggctcc cgcgccaatc aatgcgtgca gagattctct cgtttcggtg atttgccctt
    23641 tgacggcagc aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca
    23701 cttgtgcttc cagaccgtgc gccactttac tttggtgcgc tttcaccgcg taatcaatca
    23761 catcgcgagt ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa
    23821 gctgcgctac gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat
    23881 tatgcgcgcc aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca
    23941 gtccggcaaa caaacctact gtggcttccg tatagtacgg cccgtccatc cccagtgcgg
    24001 gcgcatccat cgcataaggg cttaaaaagc cgttcgtcga gacacgttgg cggttaagca
    24061 tccctaacgc tgccacctgc aactgtgaac cggcatctaa caaatcggcc tgcgattgcg
    24121 ctttttcttc ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga
    24181 tcagggcatc caactgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg
    24241 agctggcaag atgaatatca tcggccagtt tgatttgttc cggtttgagc tgttgatgag
    24301 gcgcagaatc cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc
    24361 tgcacaacag cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc
    24421 cgtggcgggc gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac
    24481 gtgttgctcc tgttttaata aggtggcagg atggcctgcg aggcgggtgt ctcttcaagc
    24541 gttatttgag gagaacggct gagtgcggtt gccagaccgg cagcgtattt ctccagggcg
    24601 tcggcaaact gacctgctct gtccggctgt agtgcgttat ccagcgcctg cgccaaattc
    24661 caggtggcat cccaggcgtg aaggatatcg cggccctgac gaataaccgt ttgcgaacgt
    24721 tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct ccagcgccac gcgttggcac
    24781 atctcttcac aggcattaaa cgccgcatgc agaccgatac gaatttgcag ataagtgcgc
    24841 agagccgtga cttcctgctg gcgtgggatc cgcattactt tacttaacat acccagcgcg
    24901 ccagccagtt tttgcggcag cgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc
    24961 atcgcggaca ccacggtacc aatgatgata cccaaggcgc gatcgcgaat ttccaccagg
    25021 tcgtacactg ggccaaaaac gttttcgagc gtggcgagcg cgaaggtgac caccatctgt
    25081 gtgccgatat aagaagagcg ttcagagctg gtagcaatcc atgcgcccaa caggaaaatc
    25141 ggtgccagca caaacagcaa ttcgacaata ttgtccagcc agggcatgac cagtagcgtg
    25201 aataacagcg ccagaatcgc gccgcaaaag gccccgccaa aacgcagcac catcttctgg
    25261 tacgacgaac cgacatttgg attagcgacg atcacgcagg tcagcataca ggtgtgaatg
    25321 ccttcccagt ccacgccgct gtaaaaggtg taacagatca aacaggcgag caatgttttt
    25381 accgcgtagc gtatatagcc tggattggta aaagcatcgg cgaccattga tggcggtttg
    25441 gcggcgggcg ttggcggcgt attcgggttc atctggccca gttgtaacaa cgtctggcag
    25501 atattctcca ggttacattc ccgtgccgcc accgcttcac tttcactgat ccgccagtcg
    25561 ctttgccagc actgaccttc agcaacggca tgctgcagct tgttgatttc tgaggctaat
    25621 ttttgccgaa attcaataat tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc
    25681 agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt gccaccatgc gctttgagtt
    25741 cgccagttgg catcgtccgc gagacaaaag acattcagtt tttgcagcgc cagcgcctct
    25801 ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg tcaggtggct aatggcatca
    25861 tcaagccgat cattaagcgc ttgatgcatt tgcgtaatgg cacgactggg aaaccacagc
    25921 acgccgatta acgtcatcag caaggttgga tagaggccaa caacgataca ccacagcgtt
    25981 aagcgcacga ctacttccgg atagtcgagc atggcgggga aggtttgccc gtaaatggcg
    26041 acaatggcga cagcgaaaaa gaccagtccc agccgatggg tgcgcatcaa aaacatgcag
    26101 cccatcagga tcggcccggc gatgatcaat cggatcaacg gttcgccgta tgaccatttg
    26161 tagatcaaaa acaggctgcc gatctccagc accgtggcaa ccacaaacaa gatcgccaca
    26221 aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca gcactgccag cgataacgcc
    26281 acaaaaggga tctcaaaagt catcgagatc agaatcacca gcaggcagcc tacccagagt
    26341 tgcacgatct gcggcacgcg gccgggtcgc cgctcgctta actcttcatg aaagaacgcc
    26401 agcagcctga ccaccggtaa tggcagggag ttgagcgcgc tcattattgc ggctcaagat
    26461 tagcgactgc cgaagcgccg atgcggaaca tttccgggtc aggtttatcg actatgattt
    26521 tgaccggaaa acgctgggca acgcggaccc agttaataga acgagacact tttggcaggc
    26581 cgcccagcac caggccgccg tcatccggta gcacgccgta gccaatcgaa tccactttac
    26641 cctcgaaggt tttgccgctg tcactcatca ggcgaatcgt tgcgggtgta cctgagcgaa
    26701 tatttttcag atcggtttcg cggaagttgg cgatcacgta ccagtgacga gtgtcgatga
    26761 gggtaaaaat agggcgcatg gcagaagcaa attgcccgac ggaggttttg agggaaatga
    26821 tccggccatc aaatggcgcg cgaacggtcg tcatttccag atgtagtttg gtcagggcaa
    26881 tatccgcttc gaccgccgca cgttgcgcca ccagcgcatc gacgccgctg gcggctgact
    26941 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    27001 ggtcaacgtc ttctgctgag acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg
    27061 tatctgtggc ctgtttcgcg gcggcacggg ctttttctac cgtggcatta accgagtcgg
    27121 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    27181 cgagggaggc ttcggctttc gccaggttgg cttcgtacgg gcgcgggtcg atgcggaaca
    27241 gcaaatcgcc ctgtttcacc gcctggttgt cggtgaccgc cagttctaca atgcgaccgc
    27301 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    27361 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    27421 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    27481 tagattagaa aaatgccagc cagaacagca tggcgtagag agcaaaaagg gcggtataaa
    27541 taattccgac aaatgccaga ttgatatttg cccgctgaat aactcgcctc gtgatgagcg
    27601 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    27661 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    27721 ttttgaggtg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtctgc
    27781 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    27841 tcgatgaata aaaatagctt tatttagcca ccgcattcat aacttatata ttttatttat
    27901 taattggctg tatatatttt taaaataaat gcttttgggg gcttcagcgc ccccaaaagc
    27961 gagtgacatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggcg
    28021 ataatttctt ccagtttcgc gccattgcca ataattttgg ctctcttcgc tttcaccaga
    28081 tccgccattt tggcctgtcc ggtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    28141 gaggcgtcga cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    28201 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    28261 ttcgcgctat cgaggcgaac ggccataaag tcgaacagca tttcgaccga catcccgcga
    28321 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    28381 ttcgcgccgg tcaggtagaa accgcgccat gtggcggact cggcctgata gcccagctgt
    28441 tcaaagttat tcgcttgcag attctttgcg acctggtcac ccgggttggc ggcaatcacc
    28501 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    28561 ttaataacgc gtgcggaacc gcccagcacc tgcacgtaac gtttacccat ctccacctga
    28621 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    28681 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    28741 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    28801 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    28861 atatgcttat tgccccagac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    28921 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    28981 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    29041 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    29101 aggaagtcaa actccaggcc gtcgataatc atcttctcgc cagttctgac gatagtttta
    29161 gtcggtgcaa taatgctcgg gtcgcccgtt gccagcgtca cgccaaggcc attacccacg
    29221 ttgccttgcg cgttgtgcgg cagtaacaga ccgtaagagt agagcgcacg gcggctcatg
    29281 atgttccccg ccagcacgtt ttcgctgatg gcttcgtcca taaagcctgc tggggcaatc
    29341 acctgaactt tgcctgattt aacatcggct tcagagataa tgcctttcac gccgccatag
    29401 tggtcggtat ggctgtgggt ataaataacc gcaacaatct gtttttgcgg gcgattttgg
    29461 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    29521 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctagtac
    29581 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    29641 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga
    29701 taatatggtt tgccattagc atcgcgcaga ataccttcat ccagcagcgg agcgataaat
    29761 ccacgctgag cgtcgtcaaa atcctgacga tcgctaaacg gtaatgattt ggcgtaattc
    29821 tgattaacct gttgggtata ttgagtggcg gctttcgctt cctctttcgc gttagcggta
    29881 tttaccatca tgccgctggc ggcagttaac gcaataacaa ttctgctcaa acggaataac
    29941 cgagagttat tcatataaat ctccatttat catttgtgtc gtaaatatgt aatccatgca
    30001 aaatacgtca tttaaagctg gggttatcat aattgggaat ttgcgacttt aataagtgga
    30061 agtgtgagcg gaacgcgcca ttttattagg catttgtgct gaagagggaa aagcacggca
    30121 tatttattac tatcatcaat aatgtgacct gacttatatt tttattattt atattacttt
    30181 ccgctttcac ttcgacaaga agtacgcaca atgattaatt agatgaatga tgcggagtgt
    30241 aaccatgaaa cccgtcgtgc tggtggtgga tgatgacacg gctatctgcg cactgttgca
    30301 ggacgtgtta agtgaacatg tctttacggt ttcagtcagc cataccggac aggaggccat
    30361 atcgcgtatt gagggcgagc ctgacattgc gctggtggtg ctggatatga tgctgcccga
    30421 taccaacggc ctgagggtat tgcagcaaat ccagaaactg cgtccggcat tgcctgtggt
    30481 gatgttgacc ggaatgggga gtgagtctga tgtggtggtc gggctggaaa tgggcgcgga
    30541 tgattacatc tgcaagccgt tcacaccacg ggtggtggtc gcacgactga aagcggtgtt
    30601 gcgatgggtt ggcgcgctgg cggttaatga ggagaaaaac ggcgggctgt cattcaatgg
    30661 ctggtatctc gataccattc gctgccaact gcatagtccg ctacaacaac atgtcgagct
    30721 gactcagggc gaatatggct tgttactggc cctggctcaa aatgcccgtc gggtattaag
    30781 ccgcgaacag ctgctggcgt tcactcatag cgacagcgtg gaggtgtttg atcgcaccat
    30841 cgacgtatta atcatgcgct tacgacgcaa aatcgaactc aacccgcatc agccgatgtt
    30901 gattaaaact ctgcgcggtc tggggtatgt cttcgctgcc gacgtgcatt aatccgacat
    30961 ttctgtttgc agctcttcca gcgttttcgc cccgtcgatg gcattggcgc ataacaggct
    31021 tgctcgcgcg caggcgtgtg ccagtttgat gctctcggta agcgtccagc gttcatggca
    31081 gccatataaa aatccggcac aaaatgcatc gcccgcaccg acgctgccga taatttcccg
    31141 ttgttctaac ttttgcgaag ggatccacac gccgcactgt tccggggtct ctccccacgc
    31201 cccttccggg cagtggatca ccacacgctg gcgcacgccc gcatctaaca gttcacgcgc
    31261 ggcggcagca atatgcgcga tatgcggttc gccgttgggt aaccgaattt ccagcccact
    31321 aaactccccg gcctccagtt cgttaattac caggtaatcc agatggcgca acgcgggcag
    31381 caccagcggc tgataacgcg gatcgccctt gcgggagacc aggtcgagtg aggtttcaaa
    31441 gccagtatca cgcatttgcg ccagcaggcg tgcgctacgg gtgccgtaaa catcgtccgg
    31501 catattcagg ctgtcgagca acagtaaata gccgagatgg aaaatcttca tggtgttatc
    31561 gagctgatcg aacgccggaa gatccaaaag tctgtttgca cctggcgaat ggaaaaacgt
    31621 ccgctgcccg cttggatcag tcattacctg cgacattgac gtcggggcaa aggtagtgcg
    31681 ctgcacatgc tgacgattga cgtgatattg ctcaagcatc gccataatgt aatcgccgtc
    31741 gctgtcctga ccgattagcc cgaccgcctg taacggcaac ccggtgcgca ttttcgccag
    31801 tgtcagcagg acgttaagcg gcgcgccgcc ggtggagcgt tcgctgtggg taatctccgc
    31861 cagccagccg cgctctggcc actggataat ctggtggacg tggtcaacca gcatattgcc
    31921 tgcggcgata atccctttac gttccatcat gcctgccccg cactgccgaa aatacgcatc
    31981 tgttgtgcaa ccgtatcgct gatggcggct tccactgcta gtagtaactc ggcaaactcg
    32041 tcatacatcg gctgacggtg cgccattcgg gcctcaacgg cagccagcgc cgcttgtgac
    32101 atgccggtgt agaaattgat tttgtggata cccagttcga tggcgcggcg gaaatcggca
    32161 tctctaatcc ctgaaccgcc gtgtaatacc agcgggatag ccgcctgctg gcggatagcg
    32221 tcgaggcgcg ggaaatcgag tttcggttcg ccgcgatatt tgccgtgcgc attgccaatc
    32281 gccacggcca gcgcgtcaat gccggtacgg tcgacaaaat cacgcgccag ctgcggatcg
    32341 gtaaacagag attcatcggc atgaccgtaa agcgcaccgc cttcgtcacc acccaccgcg
    32401 cccagttcag cttctaccga tacgccaacg gcatggcaca ttttcaccac ttcccgcgtc
    32461 tggcggatat tctcttcata ccccagcgtg gagccatcaa acattaccga gctgaatccc
    32521 agccgcaacg cttgcgccac cgattcaaaa tgcagcccgt gatcgagatt taataccacc
    32581 ggaatcgctt gacgcgccgc ttcaaatttc actgtctcga ccagggaatc cagcgagata
    32641 tatttaaaat gtacttcggc aatgttgata ataaagggcg aacgttcttg tctggcagca
    32701 gcaaacaagg cgcgtaaaaa gtgtgagtcc agcacattaa acgcacctaa cgcatagtga
    32761 tgttcgcggg catgtgccag cccttcagcg agagaaatca acggcataac gttatcctta
    32821 ttcagaggaa ctggcggtat tcgttacaca acaccagctg ggccggttcg tcttcgtcaa
    32881 tcaggttgta gcggtccagc ggttgtaaaa agtggttatc gtggtcgtcg tcattgacag
    32941 aagagacttc gccaaccaga acatcgccaa aaccggtttc cgcccaaaag ctgtgataca
    33001 ggccgggcgg caggcagata ctttcgccgg gggacaggcg taactgactg cctgcgacat
    33061 gagtctggcg gcagccgtcg atcaccaccg taacgtcggt atcaactgtt tgctcgttgc
    33121 tatcggcatt ccacagttca acaatcaaat tgccgccgcc gcgattgatg atgtcctcac
    33181 gcttacgcca gtgaaaatgc attggcgtga cctgcgcatc gcgcacatgc atgatcttct
    33241 cggcgtaaca tttcacatac ggcattcctt ttgctgagcc gttacgcagg gtaaacagcg
    33301 tcagcccctg tgctgcaaag ttgttaccac caaaagcggt aacatcccag ccgagtttga
    33361 gatcaaaaac ctcctcccac gcggcggtgt cgagctgctg ccattgagca ggagagaaac
    33421 tggcaaaggg cggcagatgt acgtcatgtt gcgaaaagaa ttgccgtgta tggccgagaa
    33481 tgttgtttat agcggagcgt ttcatcgaaa gcgttctcct tagcggtggc cgggcagcat
    33541 cgccgcccgg cgtgtggact tatttcaccg tccagccttt gtaattgccg acgttgtttt
    33601 tatcgatcat cgtcaccggg atcagcaccg gctctttcgg cgcaggtttg ccctgcaaaa
    33661 tgtcataacc gatctcaacc gctttcgctg ccatcacctg tggatcttgc gccggagtag
    33721 cgacaaacaa cgagttctca cgcttgagcg cctcttcggc gtccgggctt ccatccacgc
    33781 cgacaataaa gaactcgtta cgttgcgcct gttttgctgc cagatcggca ccgatcgctg
    33841 tcggatcgtt aatcgcaaat acgccgtcga tcttcggatt tgccgccagc agcccggtca
    33901 tcacctccag cccgccctca cgactgcctt tggcgttctt gttgtcagag agcaccttga
    33961 tgtcaggatg gcgtttaaat tcggtctggc agccttcgac gcggttttgc acggcggaca
    34021 ccggcggtcc gttgatgatc accacgttgc ctttgccttt caggcgatcg gtgatgtatt
    34081 tacaggccat ttcgcctgcc tgggtgttat cggaagtgat ggtggcatcg gcaccttctg
    34141 ccgccacgtc aaccgccacc accacgatcc cggcgtcttt tgcccgtttc accgccgggc
    34201 cgatcccttt tgaatcggca gcgttaagga tgatcatatc gacgttagcg gcgataaagt
    34261 tgtctatctg cgagacctgc tgacccagat cgtagccgct ggaaaccagc gtcactttga
    34321 cgttatcgcc cgccagtttg cgtgcttcca gctccgcgcc tttggtgatc tgtacgaaga
    34381 acgggttcgc caggtcacct accgttacgc ctatcgattt cagatctttc gcctgggcga
    34441 acggagctgc ggccagcagc gcgcctgcac agagcgcggt cactaagggc ttcaatctca
    34501 tgctgtattc ctcatgtgta gggtattgtt gttatgcact ttgatgatgt cgggtacggt
    34561 atttgtcgat aagcaccgca atgatgatca ccgccccttt gatcaccagc tgccagaagt
    34621 aagagacgcc cattaaggtc atgccattgt tgagcgtggc gataattagc gcccccacca
    34681 gcgtgccggt gatggtgccg atgccgccga caaaactggt gccgccgagg atcaccgccg
    34741 caatggcatc cagttcgtaa cccataccta agttgccgtt ggcgctgtac agccgcgagg
    34801 tgctcataat cccgccaagc cctgaaagca ggccgctcat gccataaaca aacagcagca
    34861 ccagccagac cttaatgccg gtaagacgcg ccgcctgcat attgccaccg acggcgtaga
    34921 tatgaacgcc aagcgtggtg cgacgcagaa taaaccagca cagcacgatc accgccagcg
    34981 caatcaccac cagccacggg accggcccga gatagttatt gcctatccac tcgaaattaa
    35041 tgttggagtt gataaccgtg gtgccatcag ccagcagata cgccgccccg cgcagcgccg
    35101 tataggtgcc aagcgtgacg ataaacggcg gcaatccggc aaaggccacc agcgcgccgt
    35161 tgaacagccc cagcagcagg ccgagcatca gcgccgccgg gatagacaac atggcgaact
    35221 ccgggctaag ggagacaacc attgccgcca ccgccgtggt gccgaggatt gaacctacag
    35281 aaagatcgat cccaccggtc aaaatgatga aggtcatccc cgccgccagc acaatgttga
    35341 tcgacgcctg gcgggtaata tttagcaggt tgctttcggt aaaaaagttt ggcgcgataa
    35401 agccaaatac cgctacgatc aggatcaaaa tgggcaaaat gccgaccgtt tgcatcagat
    35461 cgctcatcag catttttttg gcagaggttt ttgcagaggt cgtcatgagg tcaccgcctg
    35521 ggaatgggcg tcgttgacgc cagtcgctaa ggtcataatg ttttcctggg tgatgttttt
    35581 gccatttagc tcaccggcaa tgctgccttc gcgcatcaca tggacgcgat cgctcattcc
    35641 cactatctcc ggcagttcgc tggagattat caggatcgcc acgcccttgc gcgccatctc
    35701 gttcatgatc cggtagatct cactttttgc gccaacgtcc acgccgcggg tgggttcatc
    35761 gagcagtaaa atgcgcgggc caatcgccac ccagcgggag atcaacagtt tttgctgatt
    35821 gccgccggaa agcccgcccg cgcgtacctg ggcatgaggc acgcgaatgt tgagcaactt
    35881 aatggcgtca tcggaaatgg tttgcgcttt tttgcgattg agcattcccc agctggcatc
    35941 gcgctccagg gttgccatag tgatgttttc gactgccgcc atttcaagga ataagccttg
    36001 ttctttgcgg ttttcggtga gaaaaccaat gccaagatcg atggcttcgc gcggggagtg
    36061 gatcaccacc ggttcgccat cgacttcaat cattccgcct gttgctttcc gcacgccgaa
    36121 aattaactgc gccagttcgg aacgtccggc tcccaccagc ccggcgaggc caacaatttc
    36181 gccggaaagc acctgcaggc agctcggctg gactttgccg ccgtcggtca ggtggtgaac
    36241 attcaggcgg gctttaccga gcgggatatc gcgctcttta ttgaacagat cgctcagtgg
    36301 cctgccgacc atcatccgca ccagctcccc ggcattgagg ttatcgcggg tcaggctgcc
    36361 aacgtattgc ccgtcgcgca gcacgctgac gcgatcggaa agctcataca cttccgccat
    36421 acggtggctg atgtagataa tcgccatccc ttcatcgcgc agccgcataa tcagttcaaa
    36481 aagacggtga gtttcacggg aggagagggc agcggtgggt tcgtccatca ccagaatgcg
    36541 gctgttgcga tgtagcgcgc gggcgatttc gacctgctgt tgttcggcaa tggtcagcgt
    36601 catgacctta tcgctggcgt taaactgcgc gccgaggcgg tcgatcactc tttgcgcctg
    36661 caccagcatc tcttttcgct gcaccagccc gccgtgggaa agctcgctgc cgaggaaaat
    36721 attttctgcc accgtcaaat tgggtgccag ctgcatctcc tgataaatca gcgtaatacc
    36781 ggcgcttaag gcatcttttg gcgtgcgaat gtgaaagggc ttgccgtcta tcaggatctc
    36841 gccgctggtg gcggtatacg cccccgccag caccttcatc agcgtgcttt ttcccgcgcc
    36901 gttttccccc atcagggcat ggatctcgcc agggtagacc gtcaaatcca cccctttgag
    36961 cgcataaaat ttgccgaagg ctttggcaat attgcgcatc tcaagaaccg gcgtgctgtt
    37021 catggtggac tcctgcgagg ttgtcgatat cagcactcca tcacaggaaa gtaaatgcaa
    37081 aatgtcagaa gccgttcata tttgtcatat tgcgattttt cttcacggag cgtgatatgg
    37141 cgcaccccgg acgtcacttt tttgccagcg cgcgcgggcg attgctgctt ttgaatttgc
    37201 tggtggtggc ggtgacgttg atggtcagcg gcgtggcggt aatgggcttt cgtcatgcca
    37261 gccagatgca agagctggtg cagcagcaaa ctgtagatga tatgaccggc agcctgaatc
    37321 tggcgcgcga cacggcaaac gtggcaacgg cggcagtgcg attgtcgcag gtggtgggag
    37381 cactggaata taaaggcgaa gccgagcggt tgcaggagac acagcgggca ttaaaaagct
    37441 cgcttgcaca actggcgaac gcgcctttag cgcagcagga agccgggctg gtgacgcgca
    37501 tcatcacgcg tagcaacgaa ctgcaaacca gcgtcggcgg aatgctggaa cgcgggcaaa
    37561 ggcggcatct ggagcgtaac gcgctgttaa gttcgctgta tcaaaactta agttatctgc
    37621 ggcatctgca aaaagtcact cacgcgcaag ataatatttt gcttaacgag atgaaccgac
    37681 tgattgttgc cgccattgcc acccccgcgc cgcaggcgat tattcatcaa ctggtcggag
    37741 tgatgtctgc cttgccaact cacagcgata cgccattggt taatacgcta ctcgctgatt
    37801 ttaatcgtga actgcgtaaa ctcgcgccac tttcggcggc tctggagcaa agcgatctgg
    37861 caatcagctg gtatatgttt cacatcaaag cgctggtggc gatccttaat ggcgacatca
    37921 accgttacgc cgaacaggtg gcgacgctct ctggtcagcg ggtggagcaa agttacccgg
    37981 atttgcgctc cggcgagagg ctgatcatga ttttcgccct gttagcggtg gtgatcaccg
    38041 cgtttgcagg ttggtatatc tatcgcaatc tcggctctaa cctgacggcg atttcccgcg
    38101 cgatgagccg cctggcgcac ggcgaagcgg acgtcacttt tccggcgtta cagcggcgcg
    38161 atgagttggg tgaactggcg cgcgccttta acgtttttgc ccgcaatacc gcatcacttg
    38221 aacgaacgtc acggctgttg aaagagaaaa ccacgcagat ggagattgcc caaaccgagc
    38281 ggcaggggct ggaagaggcg ctattgcaca gccagaagtt aaaagcggtc gggcaactga
    38341 cgggcgggct ggcccatgat tttaataatc tgctggcggt aattattggt agcctggaac
    38401 tggtgaacac gaactcaccc gacgcgccgc gaatacagcg ggcgcagcaa gccgccgaac
    38461 gtggcgcgct actgacccag cgattgttgg cattttcccg caagcagtcc ttacatccgc
    38521 aagccgttga actaaaaaca ctgctggaag agttaagcga attaatgcgc cactccttac
    38581 cggcgacgct gacgattgag attgaagcgc agtctccggc gtggtccgcg tggattgacg
    38641 tcagccaact ggaaaacgcc atcatcaacc tggtgatgaa cgcccgcgac gcgatggaag
    38701 ggcagacggg aattatcaaa attcgtacct ggaatcagcg tgttacccgc agcagtgggc
    38761 agcgtcagga catggtggca ctggaggtca tcgatcatgg cagcggtatg tcgcaggcgg
    38821 tgaaaagcca ggtgttcgag ccgttcttta ccaccaaaca gacggggagc ggcagcgggc
    38881 tgggcttgtc gatggtctat ggtttcgtgc gccagtccgg cgggcgcgtg gaaattgaaa
    38941 gcgcgccggg gcaggggaca acggtcagat tacagcttcc ccgcgcgctg acggcggcga
    39001 aaaacctatc gccagcggcg gtagaacaga cagctgttaa tggcgataaa ctggtgttag
    39061 tgctggaaga tgaagccgcg gtgcgccaga ccatctgcga gcagttgcac ctgctgggct
    39121 atttgacact ggaagcgagc agcggcgaac aggcgctgga cctgctggcg gcatcagcag
    39181 aaatcgatat ctttatcagc gacttaatgc tacctggcgg catgagcggc gcagaagtgg
    39241 tcaatgcggc ccgcaaactt tacccgcacc tgacgctgct actcatcagc ggtcaggatt
    39301 tacgccccag ccataacccc gcgctgccgg acgtggcgct gctgcgcaaa ccctttaccc
    39361 gcgcgcagct ggcgcaggtg ctggggcaaa ttgagggatg attttcagaa tttatcaaga
    39421 ggctcgaagc aaacgtgctg ctattgtgag caattctgtt ctgaattgaa gtgatttaca
    39481 tatgatcgtg atgccaacca cctatagccc tatgacggtt gctcgttctt ttcgcgtggt
    39541 ggaagagatc gagctttctg gacgtctgtt acaggtaatt tatgacaacc agacgccaag
    39601 agcggccatc attgaagcgg acattgaagt ttttgcaggc gtgccgcgtc atcgggtact
    39661 tgccgctctc gatctgcagc gtaaaaaaga gttcggcagc gatatcattg ccgttgaacg
    39721 ctggtgggaa gactcggatc tggtacaggt ggaaggtatt tgtgtctctc caggtgagcg
    39781 cgataagggt ctggcaactc ggctgtatga agcgctaatc ctgcaatgcg gtatcacgct
    39841 aatcagcgat tttgatcaat atgatggcgg gaagatgctc tggcagaaga tcgcgcgcga
    39901 gtccgatcag atttccgtgt ttgtattaga cactgaacaa ggggcatttt ggccctacga
    39961 tggcacgaaa gtgatctacg atggcggctg cattccggaa gagcgtatct ggagtatttc
    40021 accggatcaa aaatgtcatg gcattgtatt ggtggcggaa aataaacagc gagctaacca
    40081 gctaatggaa gagtctgcgc cttaccgaat ttgagattac tccgctactc cggtcattca
    40141 cactcaacta acgttaagcc agtccatcgc agagactggc ttatgaaacc ttactccacg
    40201 ctttttcttt tcaccctgct cacgctgtcc accgttccgg cccaggccga tattatcgac
    40261 gatactattg gtaatattca gcaggcgatt aatgacgctt ataactctga ccgtgggcga
    40321 gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga tcggcgcaga
    40381 caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga cgatcgccag
    40441 cgccagcttg atcaggagcg tcggcagcta gaagatgaag agcggagaat ggaagatgag
    40501 tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg gggagaggga
    40561 gaaaaccagc gcaatattca atatcgagca caatcgctcc cctcgcccct ctggggagag
    40621 ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc aatctccatc
    40681 ccatcaaacc ccgcctcaaa ccccgacggc agtgcgtttt ctatcagcca cgcatcaaac
    40741 tggtggctga tatggctcag aatcacccgt ggcgagcgga taacctgatt cagcgcaagc
    40801 acggtattta aatcacagtg attacgcggc gcatccgcgc gcggcgggtg gctgcaatca
    40861 atcaccatta cctgtggctg attattgcgt aaaaatttca gcgttttttc cggcaggcca
    40921 gcggtgtcag atagccacgc cacccggcta tgtgccgttt ccagcagata accgaaggtc
    40981 agttttgagt ggttaagcgg cagcggcgtg acctgcaacc cctgcaaatc aaacaccaca
    41041 aacggttcca ctgtgtggct gaaatcaagc aggcccggat gtttaaacag atcgtcgcaa
    41101 ccctggtcat ccggcgggcc gtagatcggg atcggatcgc ccacgcccca gcgcagcgga
    41161 aacagcccct gggcgtgatc catatgataa tgcgtcagca aaaactgctg gaacgatccg
    41221 ggcgaccagc gatcggcgag atcgtgcagc ccggcgtcga tcagggtgat tgcgtcgtta
    41281 aacttcacta cgccgctgca tggctggcgg cgatactgcg gtgagcgccg cgctcggtcg
    41341 caggccgcgc actcgcagcc ccatgccgga acgccctgtg cgccgccagt gccggtgagc
    41401 gtgagggtca ggctcatgtt acagcacctt ggtgaagcgg aagtgactct gctcgtagcc
    41461 ttctcgcaga tagaaacggt gcgcgtcgtg gcgtttcacg ttggtcgaaa gcgcggtcat
    41521 ttcggccccg gcctggcggg cttcttcttc tgcccatgcc agtaatttac tgccgacgtt
    41581 cagaccgcgc gcctgcggca tcaccaccag ttcctgaatt tcgccgatcc agttgacatg
    41641 atgcagatga aactgcaaat gcaggccgat catgctgaca acttcgccat ccagcagcgc
    41701 cagatggtag cgcatgtttg ggtcgcgcag gttggcgtta aagcccacgc gaaacgcatg
    41761 gtggtcaaac tccgcctgtt ttagctcaca aatcagcgcg taaactgcgt cggtgtcgta
    41821 ctgcgtagcc gggcgaagct cacaagcagg catggtgttt ctccttctga tgaatcagcg
    41881 tcagcagctt gtcgaccgac tggcgcaggc tgccgtcatt attgagcgta agacaatcct
    41941 gaggagtata gcgggcagcg cgtgctagtc gggcgttaat ttcactggca ttttcacgac
    42001 cacggttttc gaggcgctgg cgcaggatct ccggcgaaac ctgtaagcaa atgggcagca
    42061 gcgccgattg atagcgcgcc cgcgcctgcg gcagatgggc gcgtgagccg ttgaccagca
    42121 cgtcgaatcc ggcgtgcagc cagagatcaa tctcaacgcc gacgccataa tacagactgt
    42181 tagcgtgcca gcttaaggcc aacagatttt gccccgcgcg ggtaaaaaac tcctgctcgc
    42241 tcagggcgat atggttttca cttccggcgc tggcgtcgcg cgtaatgtag cgatgcgcca
    42301 ccagtaactg agtttgttcc cgcaggctga tttccgccag caggctgtct ttcccggagc
    42361 cggacggccc cattaaccaa atcagttttc ccatcatcag aacacccttt taccctgacg
    42421 ccagacgtgg tcgatatgaa tatgattgtc cttgcgatgc gccagcacca ggtccgcgcg
    42481 tttgccctcg ccaatcaccc cgcgatcctg aagattaagc gcctgcgccg gattttgggt
    42541 cactaacttc accgcctgcg gcagcgtaaa gcggttgctc tggtcatcgg cgacgcgaaa
    42601 tgccgcatcc agcaggctgg cggggtagta gtcggaagag aggatatcca gcaggccaag
    42661 ctgcgccagt tcactggccg ccacgttgcc ggagtgcgaa ccgccgcgca cgatattcgg
    42721 cgcgcccatc agtacgttca tgccatgctt gcgcgaggct tccgccgctt caaacgtggt
    42781 gggaaattcg gcgatcacgc tgccaagcag gtgggattcg gcaacgtggg cgtgggtggc
    42841 gtcatcatgg ctggcgagcg caatttgtcg cgcgcggcac agggcggcga tggattcgcg
    42901 attcggctgc gaccagcgtg cggcaagcgc cagttgctct tcttcgtact gctgcatctg
    42961 cgcatcggtg agcgagtatt tgccctgata atattcgcga tacttctcgc ggttggcgaa
    43021 ctggcgctgg cccggcgagt ggtccatcag cgacaccagc gtcaccggct cgcgctgcac
    43081 cagtttttca aacagcggca gcgtggtgtg atgcggcagt tcgcagcgca ggtgcagacg
    43141 gtgctcggcg cggatgtcgc cgtaatgacg ggccccggtc gccatcagcc gctcgccgat
    43201 attgccgcct gccgagacct ggcgacgcag gccgtcgagt ggatgctgat gcaccacgcc
    43261 ccattcggtg cgcagcaggc gacggcggtc ggcctcgctc atcgcataca acgaacgatt
    43321 ctcgtagcga atctccccct gctgcggcgt caggcgcgcg gagattgatt tcagcagcgt
    43381 ggtcttcccg gagccggatt ccccgacgat gcccagcact tcccccggcc ataaatcaaa
    43441 agagacatcg ctaaagcctt tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag
    43501 taacggttga ttcattggtt ttttgcctcg ctctgttggc ggcaataatc ggtatcggag
    43561 cagacaaaca tgcggtttcc ggcgtcatcc agcaccactt cgtcgagata gctgtgggtc
    43621 gatccgcaga tggcgcatgg ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa
    43681 tcgagacttt ccacgcgggt aaacggcggc accgcataga tgcgcttctc gcgccctgcg
    43741 ccgaacagct gcagggcggg catcatgtcc attttcgggt tatcgaattt cgggatcggc
    43801 gacgggtcca tcacgtagcg cccgttcacc ttcaccggat aagcataggt ggtggcgata
    43861 tgaccgaagc gggcgatatc ttcatacagt ttcacctgca tcacgccgta ctcctccagc
    43921 gcgtgcatag tgcgggtttc cgtttcgcgc ggctcgataa agcgcagcgg ttcggggatc
    43981 ggcacctgga aaataactat ctgatcttcg gtcagcggcg tttcggggat gcgatggcgc
    44041 gtctggataa gcgtcgcatc gtccgtacgt tcagtggtgt tcaccccggt cacgcgcttg
    44101 aagaagttgc gaatcgacac ggcgttagtg gtgtcgtctg cgccctggtc aatcaccttc
    44161 aacacgtcgc tttcgccaat cacgctggcg gtgagctgaa tgccgccggt tccccagccg
    44221 tagggcatcg gcatctcgcg cccgccaaac ggcacctgat aaccggggat agccaccgct
    44281 tttaagatgg cgcggcggat catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag
    44341 ccgctcagat tagccatggt tctgctcctg ttgcagacgt ttgagtagct ccagttcggc
    44401 ctggaaatcg acgtagtggg ggagtttgag gtgcgagaca aagcctgcgg cttcgacgtt
    44461 gtcggcatgc gccagcacga actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta
    44521 ttccggggct tgcaacgcac ggtcaaccag cgccatcgct ttgcgctcgc tcatgccgaa
    44581 taccagcccg tagccgcgcg tgaagtgcgg cggctcatcc ggcgggtcga taaaaccgtt
    44641 gaccatttca cactcggtca tcagcagttc gccgacgttt accgcaaatc ccagctcttc
    44701 cggcacaatc gacacgtcga tgtaaccact gcggatctcg cccgcgaacg ggtgattgcg
    44761 tccgtaacca cgctgggtgg agtaggccag cgccagcaaa tagccttcgt ccccgcgcat
    44821 taattgttgc aggcgggagg agcgtgagca ggggtaaacc ggcggcgtgc gggtgatgtc
    44881 atccggctgt gcgccgctat cttcttcaaa cttcgccagc ccctgacgcg ccagcaggct
    44941 gaaaacgtgc ggcgacggct gttgttcgct gtcggcggtg gtcagcgtcg gcgcttcacc
    45001 gtttgccagc agggtgaaat cgagcaggcg atgggtgtag tcgtaggttg ggccaagcag
    45061 ctggccgccg ggaatgtctt tataaaccgc cgaaatacgc cgttcgagac gcatctcggt
    45121 ggtgtcgagc ggctcgctta ccgccagttt cgccagcgtg gtgcggtagg cgcgcagcag
    45181 gaaaattgct tcaacgttat cgccgctggc ctgtttcagc gccagcgccg ccagttcgcg
    45241 gtcggcaacg ccgccttcgg tcatcacgcg atccaccgcg agattaagct gctgttcaat
    45301 ttgggcgacg ctcagttcgg gcaaatcggt atcgccccgg cgtcggctct cttgcagggc
    45361 gtgggcggcg tcgatcgcct tttcgccccc tttcacggca acgtacatca gcacacctcc
    45421 acatgagtgg ttcgcggaat agccagcagg cgttcgccgc aggtcaggat caggtcgatg
    45481 ccgagcggga acgggtgcgg gcgctcggtg agttcgtgca gaatgcactc cggcagctgc
    45541 ggagcgatca ttcgttcttc ggcaataccc gcgccggtga gacgcaacat gcgcccgccg
    45601 ctcaggctgg cgacctgtaa aatcagcgtc gcgcccgctt ccggcgcaac ggcggtgccg
    45661 gtggaaaggg ctttgagctg tttgctggaa attccgtcat ccgtcaccgc gaaggtcgcc
    45721 tgttccggct ggctgaccag cggcgcgttg gtatgaaaac gcaggctctg attgacgata
    45781 tcgttactca atggcgcggc aagccacacc ggcgtgtcgt tatcagccag cgtcagcagc
    45841 acgctggtgg tggcgatatt cagcggctgc cagccgcgct tgagctgatg cagggcgaca
    45901 atcacgcccg gctcgcttat ggcctttaac aggcgacgaa aactgtgctg ggcatcctgc
    45961 acgggaagca taaaagcggt ttccagggtc atgcgttgtc tccgcgaacc atcgtaaaga
    46021 agtcgacccg gctggcgttc acttcggcct ggcgtgcggc aatgcgtgcc atacggtcag
    46081 cgtccagcgg ggcaataagg gtttctgata agttttgaaa gtggcgagat tgctgcatca
    46141 gcgcatcaat cagcgcgcag cgttcggtgt gctgtttatc acgccccagc acccagctgt
    46201 agccgagggt gccgtcagtc aggcgtacgg cggcgcgggt cagcgtggcg tcgccggcaa
    46261 aaaagcgttc gccggtgccg cccattcgcg cctgaatctg taccaggccg gtttcggcgg
    46321 tgcggatcac ttcatagtcg gcggtgatgt tcagcgcgtt caggcgggct gccagttcag
    46381 ccggttggct gtgcgccagt acggacatcc agtgctggcg ggtcgcggta tctgcgtgca
    46441 ttcagtgctc catagtgaat tcaatcatgt cggcgcgcgt caggctgacg gagtactccg
    46501 ccgggctgct ttcaccgtca cggtggttaa gggtgcgcac gcacagcagc ggcgacatat
    46561 tcgggatttc aaggcgctgg cactctttgg cctgggcgcg gcgggcgctg atccgcgtct
    46621 ggctgcggcg cagcgcaatt ccggtttgct cgcgcagaaa atcgtgcagc gaaccgctgt
    46681 cgaagcgttg cagcgtcggc caaagggtga ggtccgcaaa gtagtggtcg attaaacaga
    46741 gcgcgacgcc gttgacccgg cgcagggtgc gcaaatggat aacgttctcg ccctcggtaa
    46801 tccccagcgc gtcagcaacg tgaccggacg cggggcgcaa taccgacagc agtttttcgc
    46861 tggtgggatg gctgccctgg tccagcaggt tctggctaaa acgcgcctgg gcgttgagcg
    46921 ggtaatcgaa cgggcgcatc agcaccagca cgccgacgcc ctgacggcgc tgtacccagc
    46981 ctttttccac cagttggtcg atggcgcggc gcagggtgtg gcggttcacc tcaaagcgcg
    47041 ccgccagttg ctgctcggcg ggaagatagt cgccgcagcg gtagtgttga cgaagctcct
    47101 gctcaagttt tgcggctatc tcctgatagc gtgttgggta gctggtcgga tgtgtagaca
    47161 agtgcataga tatcaatgcc tcgcttatca gataaagtgc ttacgcaacc gttgagagag
    47221 gaaatccagc aggctgaccg tgacgatgat aagcaccatc agggcgcagg tttgctggaa
    47281 ctggaaaccg cgaatcgctt cccacagggt gacgccgatc ccgcccgcgc cgaccatgcc
    47341 gacgacggtc gccgagcgga cgttggattc gaagcgatag agggagtagg agatcagcag
    47401 cggcatcacc tgcggcagca cgccgtaaag gatctcttcg agtttgttgg cgccggtggc
    47461 gcgaatgcct tccaccgggc cgggttcgat cgcttccacc gcttcggaaa gcagcttgga
    47521 gagcacgccg gtggtgtgga taaacagcgc cagcacgcca gctaacgggc cgaggccgac
    47581 ggcgaccacg aacagcatgg cgaagaccat ttcgttaatg gcgcggcagg cgtccatcag
    47641 gcggcgaacg ggctggtaaa cccaccacgg caccaagttt tcggcgctca tcagaccgaa
    47701 ggggatggag agaaccactg ccagcgcggt gccccagacg gcgatttgca gcgtgacggc
    47761 catttcggtg aggtaatcct gccactggct gaaatcgggc gggaagaagt cggcagcgaa
    47821 ggttgccatg ttgccgccgt ctttaatcag cgtaagcggg gccatttccg cgccctgcca
    47881 cgagacgacc agcacggcga gaacaacggc ccagctcaga agcgagaacc agctacgctt
    47941 gggtggggcg atggtgatgg tttgcatgtt tggctccggt ctgtaggccg gataaggtgc
    48001 ttgcaccgca tccggcatca acgcctgcac attgtcggat gcgacgccgg ggcgtcttat
    48061 ccgacctacg atttactgca ccgctttact caccgaactc atcgcgctta acgcgttgtt
    48121 caggcggtcc aggtcatcca gctgcgcctg aatctcggtg gttttcgcca gcttgtcctg
    48181 ctcattcagc cctttattgc ctttcacgct ttgtatctct ttaaacagtg cgagctggcg
    48241 aatcggcacc agttgcaggt cgctggaagc gcggaacggc gcccagccca ggcgttccag
    48301 caccgctttc tcttccggcg tttttccgta gttcataaag aagtcgtaga tcttgtcttt
    48361 ggtagtttcg gacagattct tgcgccagac gatcggatcg cctaggatca gcggcgattt
    48421 ccagatcacc ttcagctctt taagcttctc cggcgcggag gttttcagct tgtcgaggtt
    48481 ttcggtgttg ttggtggcaa catccacctg cttgttggcg acggccagcg cgttggtttc
    48541 atgcccggcg ttgacggtgc gcttgaagtc gctggcggag atattgtttt tagcgaagac
    48601 gtagtaaccg gggacgagga agccagaggt ggagttagga tcgccattgc cgaaggtgag
    48661 atctttccgc ttcgccagca gatcgttcag gttgttgatc gggctgtctt tgttgacgat
    48721 caacacgctc cagtaacccg gcgatccatc cgccgcgacc gtctgggcga agacctggcc
    48781 attggcgcga tccaccgctt ccatcgccga caggttgccg taccaggcaa tatccacttt
    48841 attgaagcgc atcccctgga taatgcccgc gtagtccggg gcaaagaagg cgttgacctt
    48901 cacgcccagt ttcttctcca tatcctgtaa aaacggcgtc cattgcggtt tcaggttttg
    48961 ctgtgattcc gttgaaataa tgccgaaatt cagcgccttt tcctgctctt cggcgtgtgc
    49021 cggggttaac agggtgctga ggctgaacat gctggtgaag gccagcgagg caattatctt
    49081 agcgttcatt cgttttcctc aatgatgggg atatcaggca gctttcgcgt tctcttcgat
    49141 gcggttaatg ctgcggtaga gatggtcaaa acgttcgtta tcaaacagtt ggctgctgcc
    49201 gtcgtagaag acgtgcccct gacgcagggc gacgatgcgt tcgcagtagc gcagggcgta
    49261 atccacctga tgcagcgtga cgaccacggt gatgccgtcg ttctggttga tgtcgcgcag
    49321 ggtgtccatc acgatgcgcg ctgattccgg gtccagcgag gcgatgggtt catcggccag
    49381 aatcactttc gcctgctgca tcagcgcacg ggcaatcgcc acacgttgct gctggccgcc
    49441 ggagagggtg gaaacgcgct gatgggcaaa atgcaccatg ccaacgcggg tcagcgcctg
    49501 taacgcgcgc tgtttctgct cgccggtgaa ccagctaaaa caggtgcgcc agaacggcgt
    49561 gctgccgagc gcgccaatca gcacgttctc cagtacgctc aggcggttca ccaggttgaa
    49621 ttgttgaaag atgtagccgg tgttggcgcg gcttttgcgg atatcgcggg ccaggcggcc
    49681 ttcgcgctgg acagtgcggc ccagtagctc gatatggcta ccggcggatt tatcgccggt
    49741 aatcaaaccg cttaaatgac gtaaaagggt ggatttgccg gaacccgacg gcccaggcag
    49801 agccaccatt tcactgtgat gaatgttcag atcaaccgca tgcagcgctt gatgctgatt
    49861 gaaggttttg gcgagcttct cgacacggat aatcgtttgc atgatgcagc ctccctaaaa
    49921 aagtggctcc atcgtggcgg attattgtga cgatttggtt aattaaaagt gactaacaga
    49981 tgaagagtta acgggaattc gatgacagtg cgggagggcg ggcctcccgg cgggttattg
    50041 cgttggttgt tgtttgacga cattaatcat ccacggtacg ccaaatttat cggtgacttt
    50101 gccaaagcca tgcgcccaga aagtttcctg ccaggccatt tcgatttttc cgttagcggc
    50161 aaggttatca aaccagcgtt ttccttcttc gacctgttgc gaatcgagca ccagcgtaaa
    50221 gccggagtag ctggcttttc ctgacggtat agcatcgctc atcatgatgt cgcttccggc
    50281 aatgcgcacg ttggcatgag cgatggcggt atcgggaaat tgcattccgg aagggcagtt
    50341 ctcggcgctg tcctgcgctg atttcggcat ttcgccgaag ctgattttat agagcagttc
    50401 cgcgcccaac gtacgttgat aataggcaat cgcgtcggaa cagttaccgg caaaagagag
    50461 gtagggactt aacggcatga tggtgacctc agttaagaga agccagttaa gtgtagttcg
    50521 aaatttatac agatgagagg ctgcctgata agacgcgcaa gcgtcgcatc aggcagtcgg
    50581 cactgttgcc ggatgcggcg taaacgcctt atccggccta cggagggtgc gggaatttgt
    50641 aggcctgata agacgcgcaa gcgtcgcatc aggcagtcgg catcgttgcc ggatgcggcg
    50701 cgagcgcctt atccggctta tcgatcacaa tacaatcagt tctttttcac aaactcagat
    50761 ttcagtttca tcggaccaaa accgtcgatt ttgcaatcga tgttatggtc gccttcaacc
    50821 aggcggatgt ttttcacttt ggtgccaatt ttcagcatcg aagagctacc tttcaccttc
    50881 agatctttaa tgatggtaac gctgtcgccg tcagccagca gattgccgtt agcatcttta
    50941 acgatcagct cgtcgctttc ctgtgcaggt tctgcgtcgt tccattcgta ggcacattcc
    51001 gggcagatgt acatgccgtt atcttcgtaa gtgtattcgg agttgcattt tgggcagtgt
    51061 ggtaatgaca tgtggatttc ctcaaaagtc agcagggcaa aagcgccaaa aaacggcaga
    51121 ttgccgaaaa aggccgcaat tatacacaaa atccctgagt ttgtcggggg tactgctgat
    51181 tttagacgac gatcgttttc atttgaaagg aaacgcaaaa agaaaataat ctttttgcct
    51241 ctatttatta tttaagataa tgttaatgct ctacttgttc ttttttttat tcacatgata
    51301 tttttatctt tctgacgttt taaacgcatc agaagacgtt atttattctg ataaaaaaaa
    51361 cacactaaat ataaaattat ttcttgcagt aattataaaa gccgaaaaca gaactcaaaa
    51421 acaatctggc taaataaaag taacaaaatt tgctttaagg aaga
//
LOCUS       DADKXM010000014        51191 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.014, whole genome shotgun sequence.
ACCESSION   DADKXM010000014 DADKXM010000000
VERSION     DADKXM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.014"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..592
                     /locus_tag="JQR05_000824"
     CDS             <1..592
                     /locus_tag="JQR05_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5323049.1"
                     /translation="NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGT
                     PVLVYYLSGTINSLGSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRC
                     NTGNAGLVNVNLSLTATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPD
                     DADSIARMNVYPVSTTGVPPETGRFEATATVRINFD"
     gene            618..1145
                     /locus_tag="JQR05_000825"
     CDS             618..1145
                     /locus_tag="JQR05_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5323050.1"
                     /translation="MYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            1153..1668
                     /locus_tag="JQR05_000826"
     CDS             1153..1668
                     /locus_tag="JQR05_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5323051.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            1634..2371
                     /locus_tag="JQR05_000827"
     CDS             1634..2371
                     /locus_tag="JQR05_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY5323052.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            2482..3492
                     /gene="pyrD"
                     /locus_tag="JQR05_000828"
     CDS             2482..3492
                     /gene="pyrD"
                     /locus_tag="JQR05_000828"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY5323053.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            3666..4208
                     /gene="zapC"
                     /locus_tag="JQR05_000829"
     CDS             3666..4208
                     /gene="zapC"
                     /locus_tag="JQR05_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY5323054.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(4205..5314)
                     /gene="ycbX"
                     /locus_tag="JQR05_000830"
     CDS             complement(4205..5314)
                     /gene="ycbX"
                     /locus_tag="JQR05_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY5323055.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            5558..7666
                     /gene="rlmKL"
                     /locus_tag="JQR05_000831"
     CDS             5558..7666
                     /gene="rlmKL"
                     /locus_tag="JQR05_000831"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY5323056.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWAVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            7678..9586
                     /locus_tag="JQR05_000832"
                     /pseudo
     CDS             7678..9586
                     /locus_tag="JQR05_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9716..10969
                     /gene="pqiA"
                     /locus_tag="JQR05_000833"
     CDS             9716..10969
                     /gene="pqiA"
                     /locus_tag="JQR05_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY5323057.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGIGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            10974..12614
                     /gene="pqiB"
                     /locus_tag="JQR05_000834"
     CDS             10974..12614
                     /gene="pqiB"
                     /locus_tag="JQR05_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY5323058.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            12611..13174
                     /gene="pqiC"
                     /locus_tag="JQR05_000835"
     CDS             12611..13174
                     /gene="pqiC"
                     /locus_tag="JQR05_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY5323059.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            13430..13597
                     /gene="rmf"
                     /locus_tag="JQR05_000836"
     CDS             13430..13597
                     /gene="rmf"
                     /locus_tag="JQR05_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY5323060.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(13667..14185)
                     /gene="fabA"
                     /locus_tag="JQR05_000837"
     CDS             complement(13667..14185)
                     /gene="fabA"
                     /locus_tag="JQR05_000837"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY5323061.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(14254..16014)
                     /locus_tag="JQR05_000838"
     CDS             complement(14254..16014)
                     /locus_tag="JQR05_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY5323062.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            16199..16651
                     /gene="matP"
                     /locus_tag="JQR05_000839"
     CDS             16199..16651
                     /gene="matP"
                     /locus_tag="JQR05_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY5323063.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(16727..17773)
                     /gene="ompA"
                     /locus_tag="JQR05_000840"
     CDS             complement(16727..17773)
                     /gene="ompA"
                     /locus_tag="JQR05_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY5323064.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(18129..18638)
                     /gene="sulA"
                     /locus_tag="JQR05_000841"
     CDS             complement(18129..18638)
                     /gene="sulA"
                     /locus_tag="JQR05_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY5323065.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEKEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            18857..19486
                     /gene="sxy"
                     /locus_tag="JQR05_000842"
     CDS             18857..19486
                     /gene="sxy"
                     /locus_tag="JQR05_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY5323066.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(19449..21611)
                     /gene="yccS"
                     /locus_tag="JQR05_000843"
     CDS             complement(19449..21611)
                     /gene="yccS"
                     /locus_tag="JQR05_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY5323067.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(21621..22067)
                     /locus_tag="JQR05_000844"
     CDS             complement(21621..22067)
                     /locus_tag="JQR05_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY5323068.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            22190..24244
                     /gene="helD"
                     /locus_tag="JQR05_000845"
     CDS             22190..24244
                     /gene="helD"
                     /locus_tag="JQR05_000845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY5323069.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(24276..24734)
                     /gene="mgsA"
                     /locus_tag="JQR05_000846"
     CDS             complement(24276..24734)
                     /gene="mgsA"
                     /locus_tag="JQR05_000846"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY5323070.1"
                     /translation="MELTTRTLPTRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(24830..25492)
                     /locus_tag="JQR05_000847"
     CDS             complement(24830..25492)
                     /locus_tag="JQR05_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY5323071.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            25665..26078
                     /locus_tag="JQR05_000848"
     CDS             25665..26078
                     /locus_tag="JQR05_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY5323072.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(26123..26440)
                     /gene="hspQ"
                     /locus_tag="JQR05_000849"
     CDS             complement(26123..26440)
                     /gene="hspQ"
                     /locus_tag="JQR05_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY5323073.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(26498..27688)
                     /gene="rlmI"
                     /locus_tag="JQR05_000850"
     CDS             complement(26498..27688)
                     /gene="rlmI"
                     /locus_tag="JQR05_000850"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY5323074.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            27783..28061
                     /gene="yccX"
                     /locus_tag="JQR05_000851"
     CDS             27783..28061
                     /gene="yccX"
                     /locus_tag="JQR05_000851"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY5323075.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(28058..28387)
                     /gene="tusE"
                     /locus_tag="JQR05_000852"
     CDS             complement(28058..28387)
                     /gene="tusE"
                     /locus_tag="JQR05_000852"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY5323076.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(28478..29137)
                     /gene="yccA"
                     /locus_tag="JQR05_000853"
     CDS             complement(28478..29137)
                     /gene="yccA"
                     /locus_tag="JQR05_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY5323077.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(29344..29431)
                     /locus_tag="JQR05_000854"
     tRNA            complement(29344..29431)
                     /locus_tag="JQR05_000854"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29395..29397),aa:Ser,seq:tga)
     gene            29857..30975
                     /gene="hyaA"
                     /locus_tag="JQR05_000855"
     CDS             29857..30975
                     /gene="hyaA"
                     /locus_tag="JQR05_000855"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY5323078.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            30972..32765
                     /gene="hyaB"
                     /locus_tag="JQR05_000856"
     CDS             30972..32765
                     /gene="hyaB"
                     /locus_tag="JQR05_000856"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY5323079.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALVIVQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            32784..33491
                     /gene="hyaC"
                     /locus_tag="JQR05_000857"
     CDS             32784..33491
                     /gene="hyaC"
                     /locus_tag="JQR05_000857"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY5323080.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            33488..34075
                     /locus_tag="JQR05_000858"
     CDS             33488..34075
                     /locus_tag="JQR05_000858"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY5323081.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            34072..34470
                     /gene="hyaE"
                     /locus_tag="JQR05_000859"
     CDS             34072..34470
                     /gene="hyaE"
                     /locus_tag="JQR05_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY5323082.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQVPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            34467..35324
                     /locus_tag="JQR05_000860"
     CDS             34467..35324
                     /locus_tag="JQR05_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY5323083.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            35458..37002
                     /gene="appC"
                     /locus_tag="JQR05_000861"
     CDS             35458..37002
                     /gene="appC"
                     /locus_tag="JQR05_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY5323084.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            37014..38150
                     /gene="appB"
                     /locus_tag="JQR05_000862"
     CDS             37014..38150
                     /gene="appB"
                     /locus_tag="JQR05_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY5323085.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVASNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            38162..38254
                     /gene="cbdX"
                     /locus_tag="JQR05_000863"
     CDS             38162..38254
                     /gene="cbdX"
                     /locus_tag="JQR05_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY5323086.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            38334..39632
                     /locus_tag="JQR05_000864"
     CDS             38334..39632
                     /locus_tag="JQR05_000864"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY5323087.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQALQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(39747..41913)
                     /gene="etk"
                     /locus_tag="JQR05_000865"
                     /pseudo
     CDS             complement(39747..41913)
                     /gene="etk"
                     /locus_tag="JQR05_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(41933..42379)
                     /gene="etp"
                     /locus_tag="JQR05_000866"
                     /gene_synonym="yccY"
     CDS             complement(41933..42379)
                     /gene="etp"
                     /locus_tag="JQR05_000866"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY5323088.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(42367..43506)
                     /locus_tag="JQR05_000867"
     CDS             complement(42367..43506)
                     /locus_tag="JQR05_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY5323089.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(43552..45648)
                     /locus_tag="JQR05_000868"
     CDS             complement(43552..45648)
                     /locus_tag="JQR05_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY5323090.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(45648..46394)
                     /locus_tag="JQR05_000869"
     CDS             complement(45648..46394)
                     /locus_tag="JQR05_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY5323091.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(46391..47035)
                     /locus_tag="JQR05_000870"
     CDS             complement(46391..47035)
                     /locus_tag="JQR05_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY5323092.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(47142..47447)
                     /locus_tag="JQR05_000871"
     CDS             complement(47142..47447)
                     /locus_tag="JQR05_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323093.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(47889..48101)
                     /gene="cspH"
                     /locus_tag="JQR05_000872"
     CDS             complement(47889..48101)
                     /gene="cspH"
                     /locus_tag="JQR05_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY5323094.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            48387..48599
                     /gene="cspG"
                     /locus_tag="JQR05_000873"
     CDS             48387..48599
                     /gene="cspG"
                     /locus_tag="JQR05_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY5323095.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            48610..48798
                     /locus_tag="JQR05_000874"
     CDS             48610..48798
                     /locus_tag="JQR05_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY5323096.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            48773..49003
                     /gene="ymcE"
                     /locus_tag="JQR05_000875"
     CDS             48773..49003
                     /gene="ymcE"
                     /locus_tag="JQR05_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY5323097.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNIPYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            48993..49166
                     /locus_tag="JQR05_000876"
     CDS             48993..49166
                     /locus_tag="JQR05_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY5323098.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(49215..50288)
                     /locus_tag="JQR05_000877"
     CDS             complement(49215..50288)
                     /locus_tag="JQR05_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY5323099.1"
                     /translation="MAEKKRTRWQQRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVTDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(50390..>51191)
                     /locus_tag="JQR05_000878"
     CDS             complement(50390..>51191)
                     /locus_tag="JQR05_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY5323100.1"
                     /translation="AMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEA"
BASE COUNT        12652 a        12646 c        13397 g        12496 t
ORIGIN      
        1 taatagcggt agcttttccc tgaaaatatt gcagccgttc gttggttcgg tcgtcattcc
       61 tcctatggcg ctggcgcgat tatttgaatg ctacaacata cccgcaggtg attcctgcac
      121 gactactggt acaccggttt tagtgtatta cctgtctggt acgatcaatt cacttggctc
      181 atgttccgtc aatgccggag aaacaatcga ggttgatctg ggcgacgtat ttgcggctaa
      241 ctttcgtgtt gtagggcata agcctcttgg ggccagaacg gcagaacttg caattccggt
      301 caggtgtaac acgggaaacg cagggttagt taacgtcaat ctgagtctga cggcaacctc
      361 agaccccagc tatccccagg cgattaagac gtcacgtcct ggcgtgggcg tggtggtgac
      421 cgatagccag aacaacatta tttcccctgc tggtgggaca ctaccgctct ctattcctga
      481 tgatgcagac agtatcgcgc gaatgaatgt ctatccagtc agcacgacag gcgtaccacc
      541 agaaaccggg cgatttgaag ccacggcaac ggtgagaata aattttgatt aacgcgtgaa
      601 cgtatgaaga aaaaacgata tatcagtgcg ttattttgtt ctttagcctt cttaacatcc
      661 atgtcgggat ggctgggcct gaacaagtca gtatgcatat ttatgggaat gtggtcgatc
      721 agggctgtga tgtcgccacc aaaagtgcat tacaaaatat tcatattggt gattttaata
      781 tcagtgattt tcaggccgcg aataccgtaa gcactgctgc tgatttgaat attgatatca
      841 ccggttgtgc cgctggtatt actggcgcgg acgtcctttt tagcggcgag gctgacaccc
      901 ttgcgccgac actgctcaaa ctaactgaca caggcggaag cggtggtatg gcaacgggga
      961 ttgccgtgca aattcttgat gcgcaaagtc agcaagaaat cccgctcaat caggtccagc
     1021 ctcttacgcc cttaaaagcc ggggataaca cactcaaata tcaacttcgt tataagtcca
     1081 caaaggcggg agcaacgggc ggtaatgcga cggcggttct ctattttgat ctggtttacc
     1141 agtgaaggag tgatgttgaa acggataatc tggattctgt tcttactggg attaacgggg
     1201 ggctgtgagc tatttgccca tgatggcacg gtcaacatta gcggatcgtt tcgccgtaat
     1261 acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc agttaggcga tgtctcgctg
     1321 acacgttttt ttcatgggaa ttatggcccg gagaaatctt tcatcattaa tttgcaggat
     1381 tgcggaacag atgtcagcac ggttgacgtc accttttcag gaacccccga cggagtgcag
     1441 agcgaaatgc tgtccattga aagtggtacg gatgcagcca gtgggctggc aattgcgatt
     1501 ctggatgatg cgaagatatt gattccgctt aaccaggcga gtaaagacta cagcctacat
     1561 agcggtaagg tgccattaac tttttatgcg caattgcgac ctgtcaatag cgatgtgcag
     1621 tcgggtaaag tgaatgccag cgcgacattt gtacttcatt atgactaata cgtggaacag
     1681 attagcgctc ttgattttcg ccgttttatc gctgctggtg gcgggtgaat tgcaggcagg
     1741 agtggtggtc ggcggaacgc gatttatctt tccggcagac agagaatcga tatctatttt
     1801 actgactaat accagtcagg aatcctggct tattaacagc aaaatcaatc gcccaacgcg
     1861 ttgggccggg ggggaagcgt cgacagtgcc agcactatta ctggccgctc cgccacttat
     1921 tctcctgaag cccggtacta caggcacgtt gcgcttgctg agaacggaaa gcgacatctt
     1981 gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc agcgtgccat ccggcaaagt
     2041 tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt aaactgttct ggcgacccga
     2101 aggtttgccg ggcgacccgc tggaagctta tcaacaatta cgctggacac ggaattcgca
     2161 gggtgtacaa ctcactaacc caacgcctta ctacattaac ctgattcagg tgagcgtaaa
     2221 tggtaaagcg ttaagtaatg cgggagtcgt gccgcctaaa agccagcgtc agacaaactg
     2281 gtgtcaggta atagcaccgt gtcatgtcgc ctggcggacg ataaatgatt acggcgggtt
     2341 gagtgcaaag aaggagcaaa atctgccctg aaacaggttc tgaaaacgtt tgcgtttttt
     2401 ttgccgcagg tcaattccct tttggtccga actcgcacat aatacgcccc cggtttgcac
     2461 accgggaatc caggagagtt catgtactac cccttcgttc gtaaagccct tttccagctc
     2521 gatccagagc gcgctcatga gtttactttt cagcaattac gccgcattac cggaacaccg
     2581 tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg ttaactgcat gggcctgacg
     2641 tttaaaaatc cgcttggtct ggcagccggt cttgataaag acggggagtg cattgatgcg
     2701 ttaggcgcga tgggatttgg atcgatcgag atcggtaccg tcacgccacg tccacagcct
     2761 ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg aaggtttgat caaccgtatg
     2821 ggctttaata atcttggcgt tgataacctc gtagagaacg taaaaaaggc ccattatgac
     2881 ggcgtcttgg gtattaatat cggcaaaaat aaagatacgc cagtggagca gggcaaagat
     2941 gactatctga tttgtatgga aaaaatctat gcctatgcgg gatatatcgc catcaatatt
     3001 tcatcgccga ataccccagg attacgcacg ctgcaatatg gtgaagcgct ggatgatctc
     3061 ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga tgcaccataa atatgtgccg
     3121 atcgcagtga agatcgcgcc ggatctttct gaagatgaat tgatccaggt tgccgatagt
     3181 ttagttcgcc ataatattga tggcgttatt gcaaccaata ccactctcga tcgttctctg
     3241 gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta
     3301 aaaagcacag aaattattcg ccgcttgtca ctagaattaa acggtcgctt accgatcatc
     3361 ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa agattgctgc gggtgcctca
     3421 ctggtgcaaa tttattctgg ttttattttt aaaggtccgc cgctgattaa agaaatcgtt
     3481 acccatatct aattatttct tcgaattcct ctttataaga taatcagggc tttattttca
     3541 gccctggttg ttttatattc atcgttgttg cttatttaga cattttgtac ttttattgtt
     3601 gaggttatta agcgaagcga caatggattg tgttgctgcg gttttatagg gtaggggaga
     3661 ggcagatgcg aattaaacca gacgataact ggcgttggta ttacgatgaa gagcacgatc
     3721 gaatgatgct cgatttagcc aatggtatgc tatttcgctc acgttttgcg cgcaaaatgt
     3781 tgactccaga tgccttttct cccgcaggct tttgcgttga tgatgccgcg ctctatttct
     3841 cttttgaaga aaagtgccgc gattttaatt tatctaaaga ccaaaaagcc gaactggtgc
     3901 tgaatgcact ggtagcaatt cgttatctca aaccgcaaat gccgaaaagc tggcattttg
     3961 tctcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg
     4021 atactcacga gcaggtcaat ttgctggtcg ttgaatctgg cgaaaatgcc gcgctatgcc
     4081 tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat gcagttgggc gacgccatta
     4141 aaatcatgaa cgacaggctg aaaccgcagg tgaatgttga cagcttcagc ctcgaacagg
     4201 cagtctaacg cgtcagctta agggcagtct tcggtacaca gctacagcaa agaatggtgc
     4261 catcatcgcc cattgctgat tttttcagcg gcgtgacttc gccttctaaa agctgaacac
     4321 ggcaacttcc acaaatgccc gcgcggcaag aataagggat acgaattccc tgattttcta
     4381 attgctccag caacacttgt tggttatttc cacgaaatgc ctgtccctgc cagtcaatat
     4441 ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt
     4501 aaatttttgc cggagccgtt gccagaattt ccacctcatc gccaacgcga atcacgccgc
     4561 tattacgggc aattaaattc tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga
     4621 aagattgcaa tgtttttaat ggttcgcctg ccggatgttt ttgccctttt tctgggctga
     4681 cggtggtgaa aatacagcgg ctacaaggtt taaccacatc aaacaccaca tcaccaatgc
     4741 gaatcacttt ccagctatct tcttcccagg ctgacgcgcc ggaaaccacc agattggggc
     4801 ggaattgctc catttttaca ctggctggac aacgttgttg gagatcacgt aacgaggctt
     4861 cgttagcaag aaggtaagga tagccatcag caaatgacag aggtacagtg ttgtggcgtt
     4921 tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc
     4981 cacttagcca tttgttgatc gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa
     5041 cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg ctactgccat
     5101 ctggtgcggt gagatgcaag ccatcatgca caggtgaagg agtaaaccgt accatctggg
     5161 gaaactggcg agcggtaata aacgtaccgt caggttccgt gatcataaag atgcgatcga
     5221 aggccagacc actgacatca gccagagcat gtgtaagacc aatgccgcgc atcgatttaa
     5281 caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg gtcctcaaat gaaaataagc
     5341 cctcaacttt atgacatgac gcgcttatta gctataatgc gcaacaattt tcttagcgcc
     5401 tgtcccgtta ggctatttta tttgtcagtt tggccctggg ttgtgctcga aatcctcacg
     5461 tacttgtgta cgctccggtt tctccgcgct ctccatgtcc agactgactg caacaattac
     5521 gcctactgcg gtaggctctt agagtaaaag tgacgatatg aattctctgt ttgccagtac
     5581 ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa aatctggggg ccgttgaatg
     5641 ccaggtggtt cagggtgggg tccatttcaa gggcgacaca cggcttgttt accagagcct
     5701 gatgtggagc cgcttggcct cgcgtattat gttgccgctg ggcgagtgta aggtttacag
     5761 cgatttagac ctctatctcg gtgttcaggc gatcaactgg acagagatgt ttaatcctgg
     5821 cgcgaccttc gctgtccact tcagtggttt gaatgacacc atccgcaaca gtcagtacgg
     5881 cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc
     5941 aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc tggctgcata aagaaaccgc
     6001 cagtatcgct cttgatctga gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc
     6061 tggtattgcg ccgatcaaag aaaccctggc agccgctatt gtgatgcgat ccggctggca
     6121 gtcaggaaca ccgctgctcg atccgatgtg tggttccggt acgttgctga ttgaagcggc
     6181 gatgctggcg accgatcgcg caccaggctt gcaccgtggg cgttggggct ttagcggctg
     6241 ggcgcagcat gatgaagcta tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg
     6301 taaaggcctg gctgagtata gctctcattt ttacggttcg gacagcgacg cacgggtgat
     6361 tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt
     6421 gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg ccgtacggta cagtgttgag
     6481 caacccgcca tacggtgaac gtctggacag cgaaccggcg ctgattgcgc tgcatagtct
     6541 gctgggccga atcatgaaaa accagttcgg tggctggaat ctctctttgt ttagtgcctc
     6601 gccggatctg ctaagctgcc tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg
     6661 cccgctggac tgcgtacaga aaaattacca cgttgccgaa agcacaccag acagtaaacc
     6721 ggcaatggta gcggaagatt acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa
     6781 gtgggcacgc caggaaggga ttgaatgtta ccgcctgtat gacgccgatc tgccggaata
     6841 taacgttgcc gttgaccgtt atgccgactg ggcggtggtg caggagtatg cgccgccaaa
     6901 aactattgat gcgcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc
     6961 ggtactgggg attgcgccaa acaaactggt gctgaaaacc cgtgaacggc agaagggcaa
     7021 aaaccagtac cagaaactgg gcgagaaggg cgagtttctt gaagttaccg aatataacgc
     7081 tcacttgtgg gtgaacctga cggattatct cgatactggt ctgttcctcg atcaccgtat
     7141 cgcccgccgt atgctcggtc agatgagcaa aggtaaagat ttcctcaacc tgttttctta
     7201 taccggcagc gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga
     7261 tatgtcgcgt acttatctgg aatgggcaga acgcaacctg cgtctgaatg gcctgaccag
     7321 gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca
     7381 gttcgatctg atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc
     7441 gtttgatgtt cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc
     7501 aggtgggacg atcatgttct cgaacaacaa acgtggcttc cgtatggatc tcgacggcct
     7561 ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc
     7621 ccgtaaccgc cagatccaca actgctggct gattaccgca gcctgaaagg aatagtaatg
     7681 tcattaatca gtatgcatgg cgcatggctg tcgttcagcg acgcgccgct tctcgataac
     7741 gcagaactgc atatcgaaga taacgaacgt gtttgtctgg tgggccgcaa cggcgcaggc
     7801 aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc tggatgacgg tcgcattatt
     7861 tatgagcaag atttgattgt agctcgtctg caacaggatc cgccgcgtaa cgttgagggt
     7921 agcgtttatg atttcgttgc cgaaggcatt gaagaacaag cggaatatct gaaacgctat
     7981 cacgatattt cgcgcctggt gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg
     8041 aaggttcagg aacagctgga tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa
     8101 gtgctggcgc aactggggtt agatcctaac gttgcgctgt cgtcgctttc cggcggctgg
     8161 ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc cgcgcgtatt gctgctggac
     8221 gaaccgacta accatctgga tatcgaaacc atcgactggc tggaggggtt ccttaaaacc
     8281 ttcaatggga cgattatttt catctcccac gaccgttcgt ttatccgcaa tatggcgacg
     8341 cgcattgttg atctcgatcg cggcaagctg gtgacctatc cagggaatta cgaccagtac
     8401 ctgcaggaaa aagaagaagc cctgcgcgtg gaagaactac aaaatgccga gttcgatcgc
     8461 aaactggcgc aggaagaagt gtggattcgc taggggatca aagcacgccg tacccgtaat
     8521 gaaggccgcg tacgcgccct gaaagcgatg cgtcgcgaac gtggtgaacg tcgcgaagtg
     8581 atgggtaccg caaagatgca ggtggaagag gccagccgct ccggtaagat cgttttcgaa
     8641 atggaagacg tttgctacca ggttaacggt aagcaactgg tgaaagattt ttctgcccag
     8701 gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg
     8761 ctgaaactga tgctcggtca gcttcaagcg gacagcgggc gtattcacgt tggcaccaaa
     8821 ctggcaagtg gcttatttcg atcagcaccg cgcggaactg gatcccgata aaacggtgat
     8881 ggataacctt gccgaaggta agcaagaggt gatggttaat ggcaagccac gccacgtatt
     8941 gggctatttg caggactttc tgttccatcc gaaacgggcg atgacgccag tacgtgcgct
     9001 ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg ttcctcaaac caagcaactt
     9061 attgattctt gacgaaccga ccaacgatct tgatgtcgaa acgctggaac tgctggaaga
     9121 actgatcgac agctatcagg gcacggtatt gctggttagc cacgatcgtc agtttgtcga
     9181 taacaccgtt acagaatgct ggatcttcga aggcggcggt aaaattggtc gttatgttgg
     9241 cggttatcat gatgcccgtg gtcagcaaga acaatatgtg gcactcaaac agcctgcggt
     9301 gaaaaaaagc gaagaagccg ccgcgccaaa agcagaaact gtaaaacgca gcagtagcaa
     9361 actaagctat aaattgcagc gcgaactgga gcagctaccg caattgctcg aagatctgga
     9421 ggcgaagctg gaagccctac agacgcaagt ggcggatgct tccttcttca gtcagccgca
     9481 tgagcagacg caaaaagtgc ttgctgatat ggctgctgca gagcaggagc tggagcaagc
     9541 ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg
     9601 atttaaacag agacagctga caacggtaaa attgcctgat gaactgggtt tatcaggcat
     9661 gaaaaccaac actttgttag tcatctgtaa aatagcgcat cattaaggag taccaatgtg
     9721 cgaacatcat catgccgcga agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt
     9781 accgcgcctt gagcatggtc agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt
     9841 ggcgtgggat gcccctcggc agcgtcccac cgcctatgcg ttggctgcac tgttcatgct
     9901 gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta ccagtgaaat
     9961 tacattactg gaaattcccg gcgtgctttt ttctgaggac tacgccagcc tcggcacctt
    10021 tttcctgttg tttgtgcaac tggttcccgc gttttgtctg ataaccattc tgttactggt
    10081 gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg gcacgggtgc tttttcaact
    10141 caaaacctgg ggaatggcgg agattttcct cgcgggtgtg ctggtcagtt tcgttaaact
    10201 gatggcttac ggcagcattg ggataggcag cagctttctc ccctggtgtt tattttgtgt
    10261 cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg acatcgcccc
    10321 gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc aggggctgcg
    10381 ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc cgcgttgtag
    10441 taccaaaggg tacgttcggc gtagaaacag cctgcagtgg acactcgcgc tgcttgtaac
    10501 gtccatcatg ctgtatcttc cggctaatat tttgcccatc atggtgacgg atttattagg
    10561 ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg aaggctctta
    10621 tcccgtcgct gcggtgatct ttctggccag tattatggtg ccaacgttaa agatgatcgc
    10681 catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg aaagaatgca
    10741 tttgatttat gaagttgttg agtttgtagg ccgctggtcg atgattgacg ttttcgttat
    10801 cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg agtatttatc cggcaatggg
    10861 tgcattaatg tttgctttag tcgtcataat gacaatgttt tctgctatga cgtttgaccc
    10921 gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag gagtcctgac agtatggaat
    10981 ctaataatgg ggaagccaaa atccagaaag tgaagaactg gtctcccgtg tggatatttc
    11041 ctatcgtcac ggcgctcatt ggggcctggg ttctttttta tcattacagc catcagggac
    11101 cggaagtgac cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa accaccatta
    11161 aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac actggctgat gatttgacgc
    11221 acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa attgctgcat aaagacaccg
    11281 tcttttgggt ggtgaaaccg cagattggtc gcgaagggat tagcggcctg ggaacgctgc
    11341 tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg
    11401 atttgctgga ctcaccaccg ttggccccgc ctgatgcgaa aggtatccgt gtgattctcg
    11461 atagcaaaaa agccgggcag ctctcgccag gagatccggt gctgttccgt ggctatcggg
    11521 taggttcggt tgaaaccagc accttcgata cgcaaaaacg caatatcagt tatcaactgt
    11581 tcatcaatgc accttatgac cgactggtga ccagcaatgt tcgcttctgg aaagatagtg
    11641 gcattgcggt tgatctgacg tcagcgggga tgcgtgtgga gatgggctca ttgacaacgc
    11701 tgcttagtgg cggtgttagc tttgatgtgc cggaaggtct ggatttaggg cagccagtgg
    11761 caccgaaaac agctttcgtt ttgtatgatg atcagaagag cattcaggat tcgttgtaca
    11821 ccgatcacat tgattatctg atgttcttta aagattcggt acgcggtctg caaccgggag
    11881 ctccggtaga attccggggt attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc
    11941 cgaatatgcg tcagacattt aacgatgatt accgtattcc ggtactgatt cgtatcgagc
    12001 cagagcggct gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac cttggcgaat
    12061 tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa cctggtcact ggtgcactgt
    12121 atgttgatct cgatttctat ccaaatacgc ctgcaataac cggtattcgt gaatttaatg
    12181 gttatcagat tatcccgact gttagcggcg gcctggcgca aatccagcaa cgactgatgg
    12241 aagcgttgga taagatcaac aaactgccat tgaatccgat gattgaacag gcaaccagta
    12301 cgctttctga aagtcagcgc acaatgaaaa acctgcaaac gacgctggat agcatgaaca
    12361 agatcctcgc cagccagtcg atgcagcagt tgccgacgga tatgcagtca acgttgcgtg
    12421 aattgaatcg cagcatgcag ggcttccagc ctggctccgc agcctacaac aagatggtgg
    12481 cggatatgca gcgccttgat caggtgttgc gagaactgca accggtgctg aaaacgctca
    12541 atgagaagag taacgcgctg gtatttgaag cgaaggacaa aaaagatcca gagccgaaga
    12601 gggcgaaaca atgaaaaagt ggctagtgac gattgcagca ctgtggctgg ccggatgcag
    12661 ctccggcgaa attaataaaa actattacca gttacctgtg gtgcagagcg gtacacaaag
    12721 taccgccagc cagggcaatc gtctgttatg ggtagagcag gtcactgttc ctgactatct
    12781 ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa
    12841 cttgtgggcc agcccgttgg atcaacagtt gcgcaacacc ctggttgcca acctgagtac
    12901 gcaactgccc ggctgggtgg ttgcctccca gcctctggga agcgcccagg acacgctcaa
    12961 tgttaccgta acggagttta acggtcgcta tgatggcaag gtcattgtca gtggtgagtg
    13021 gctgttgaac caccagggac aactgatcaa acgtccgttc cgtctggaag gagtgcaaac
    13081 tcaggatggt tacgatgaga tggttaaagt gctggccggt gtctggagtc aggaagccgc
    13141 ttctattgca caagagataa agcgtctacc ttaattataa agatttgtaa atataaccgt
    13201 ctccggtatg ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa tttgttcctc
    13261 ttcacatttt ttgtacaacc gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa
    13321 ttttgcgcat tgacggcagt tatgattcgc ggtattgctt aactgtgatt gcacatttag
    13381 taatcactgt tttcttttcc accagaaacc agtatgaggg aaacgaggca tgaagagaca
    13441 aaaacgagat cgcctggaac gggcacatca acgtggttat caggccggca tcgccggacg
    13501 ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg tcacaatggc tgggaggctg
    13561 gcgagaagcc atggcggaca gggtagtaat ggcctgattc tgtctcttta aaaagaaacc
    13621 tccgcattgc ggaggtttcg ccttttgata ctctgtctga tgataatcag aaggcagacg
    13681 tatcctggaa cagaccgact ttcaggtcgc tggcggtata gatcagacga caatcaacca
    13741 gcacttcgcc atctgccaga cccataatca gacgacgatt aacaatgcgt ttaaagtgaa
    13801 tacggtaggt cacttttttc gctgtcggca gtacctgacc agtgaatttc acttcgccaa
    13861 cgcccagcgc gcggccttta ccttcgccgc ccagccagcc gaggtagaac cctaccagct
    13921 gccacattgc gtccaggccc aggcatcccg gcataaccgg atcgccaatg aagtggcatc
    13981 cgaagaacca cagatccgga ttgatatcca gttctgcttc aacataccct ttgtcgaagt
    14041 taccacccgt ttcggtcatt ttgaccacac ggtccatcat cagcatgttc ggtgctggca
    14101 attgcgggcc tttagcgcca aacagttcac cgtgaccaga ggcaagaagg tcttcttttg
    14161 tataggattc gcgtttatct accatgttct ctgtaagcct tattttattg aagcacgcag
    14221 gatagctaac acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc agttcagcca
    14281 acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg cgatacgttc
    14341 ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat ttaataataa
    14401 cggcagtgcg tcagtcacat cgtctactgc ccagatggtg aatttgcctt cttctaccgc
    14461 tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta gcagtgggga taataacacc
    14521 ttgtttcccc gttaactcac gttgctggca aatagcaaag aagccttcga ttttctcatt
    14581 taaaccaccg accggctgga cgcggccgaa ctgatcgact gaacctgtga tagcgatact
    14641 ctgattcact ggcacatcag cgagggcgct tatcagggcg cagagttcag ccatcgaggc
    14701 actatcgcca tccacttcac tgtatgactg ctcaaatgtc agcgatgctg agaaggggat
    14761 ctgttgctca agctgtagtt ccgacatcag gaacgcctgc atgatcatca tccctttcgc
    14821 atggatattg ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac catcgccaat
    14881 atgcacaacg cagctaatgc gagaaggttc gccaaaagcg cgtggatgac ccggaaattc
    14941 aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg
    15001 ctcctgaagg atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc attcacgctg
    15061 ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg ccatcacaca gggaggcgac
    15121 ctctttacac tggcggagga tccactgcgg gctaagcgga agtgtttctt gttcaccggt
    15181 gtagcgtgct gcttcgcgga taagtaccgg ccaagcatcc gctcccgggg caggtaagtg
    15241 attatgtctg gcggtaaatg tcacccagcg acaccactgg cttactgact ccgcatcgac
    15301 aatctgcaga gtatcttcaa attcgctata aatagcctgc tctgaaagct ctggctccat
    15361 ctcctggaaa tcagccaatg attcgcgttc gcctaccaga atgactttca gcttcaatgg
    15421 catcgaaggc acagagacgg ggagagggcg cgactcatca aacgcaaccc agtcaaaacg
    15481 ctcgcggtta acgatatttt tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg
    15541 caaagagatg atgagaatac cgccatttgc ctgatgcacc agaccaggct gcagggtaat
    15601 gtcgccatta aactggcgca ggcagccaaa gagttgctcc gcttctaccc agtcggcagc
    15661 gacaacttgc gttaaagtcg caaaattatc atctgcactc actgcgtgac gtaagcggat
    15721 ggagtggccg gaaacctcat agtgatcgcc caccagttgg cctgcatcgc tttgtagcgt
    15781 acgcgcggca tcggcaataa gattcagata ctcagactct tccggggcct tagccagcat
    15841 aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca gtcgcggttg
    15901 agtatcactg aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg
    15961 atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga
    16021 tgttttttag tctgttgtca aagccgcgat tataccgtaa ccggcactac agcacacgta
    16081 gaaagcaccg acaatactct tggctgggcg ttaaagctca caggatggag attcttttct
    16141 tcactggcct aaaaagctga tattctgtaa agagttacac ggtaacattg agatcgctat
    16201 gaaatatcaa caacttgaaa atcttgaaag cggttggaaa tggaagtatc tggtgaagaa
    16261 gcatcgcgaa ggggagttaa ttacccgtta catagaagcc agtgccgccc aggaagccgt
    16321 cgatgtgttg ctctcgctcg aaaatgaacc cgtgctggta aatggctgga ttgacaagca
    16381 tatgaatccg gaactggtca atcgcatgaa gcagaccatt cgggccagac ggaagcgcca
    16441 ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc gatctggaat ttattgtctg
    16501 gcaacgtctg gctggtcttg cacagcgtcg cggtaaaacg ctttctgaaa cgattgttca
    16561 gctgattgaa gatgcggaaa acaaagagaa atacgcgaat aaaatgtctt ctctgaagca
    16621 ggatctgcag gcattgctgg gtaaggaata actgacgaaa gtcagttcaa tttactaaag
    16681 gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac gataacttaa gcctgcggct
    16741 gagttacaac gtctttgata cctttaactt cgatctctac gcgacgatcc ggagccaggc
    16801 agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt gttgccagta accgggttgg
    16861 attcgcccat accacgtgcg gagatcttgt ctgccgggat acctttggag atcaggtaat
    16921 caacaacaga ctgagcacgg cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc
    16981 ggtcagtgta acccagaaca actacggaac cgtctttcgg atccaggttg ctcagctggc
    17041 tgtacagctg atccagagca gcctgacctt ccggtttcag ggttgctttg ttgaagttga
    17101 acagaacgtc agacttcaga gtgaagtgct tggtctgtac ttccggtgcc ggagctggag
    17161 caactaccgg agctgcttcg ccctgaccga aacggtagga aacacccagg ctcagcagac
    17221 cgttgtccgg acgagtaccg atggtgtttg cgtcaccgat gttgttggtc cactggtatt
    17281 ccagacgggt agcgatttca ggagtgatcg cgtactcaac accacctgcg aaaaccggag
    17341 aaacgccagt gtcgtggtct ttaaaggatg cgccaccagg tacgttagcc ttggtgtctg
    17401 cacgccatac cataccaccc agacgagtgt agatgtccag atcgtcagtg attgggtaac
    17461 ccagtttagc ggtcagctga acgccctgag ctttgtatgc gccgttgatg ttgtcgcctt
    17521 tgtacggcat acgacctaac cagtcgtaac ccatttcaaa accaacatac gggttaacct
    17581 ggtaaccacc aaaagcacct gcacccagtt ggttttcgtg ggtcggacca ttgttaggaa
    17641 taaaaccagt gtcatggtac tgggaccagc ccagtttagc accagtgtac caggtgttat
    17701 ctttcggagc ggcctgcgct acggtagcga aaccagccag tgccactgca atcgcgatag
    17761 ctgtcttttt cattttttgc gcctcgttat catccaaaat acgccatgaa tatctccaac
    17821 gagataacac ggttaaatcc ttcaccgggg gatctgctca atattaactc taccgatatc
    17881 ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg aaaacttaca
    17941 agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt
    18001 tgtcagcgac aatttacaga aaaaaaacgc ggaaaccgct tcagacaagc cttcgcaagg
    18061 aaaattagtc acgactgaaa gcattggctg ggcgacaaaa aaagttccag gattaatcct
    18121 aaatttactt aatgatacaa attagagtga atttttagcc cggaaagttg tctcgttgtg
    18181 tgagaggatg cgcttaccgg gcgcataata aaccccatag cgttaccttc atttgccgca
    18241 tcaacaagtt cagcatgctc tttttcagtc aaatcatctg tcaaccaacc gatcaccaca
    18301 ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca aggggagagc
    18361 tggctaatct gcattacttt cgttaagggt agcccagatg cctgaaccca ttcccgactc
    18421 agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag ttgctgtaac
    18481 aatggcaaca gtagaagttg cgtcatcatg ggctgatctt cgcgatagac aacttcactg
    18541 ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc tgcggatgag
    18601 aacgacgaag aacgatgtgc atagcctgaa gtgtacataa tcaatccagc ccctgtgagt
    18661 tactgtatgg atgtgcagta catccagtga caacaaagat caaccctatt ttcggaaaga
    18721 gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat taatcttgcc ccttaagaat
    18781 aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt tatgttatga
    18841 ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat cacaagaata
    18901 cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca gcctgaccgt
    18961 tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg cttgtgagca
    19021 aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa agtgtggccg
    19081 atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc aactgaagct
    19141 ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca cgcgcaatac
    19201 ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga ttctcgggga
    19261 ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt gttggttgcg
    19321 actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg aaggtgccat
    19381 tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg cagaatgggc
    19441 tgactctctt acgccgaaac aggagtttcc tgcggagctt gagtaatctg gcgttgcaga
    19501 cgaccaatct ctggcagtaa tgcaatcaat aacccaactt gttgtacgac cagaggttct
    19561 ttgctatctg cccgtggttc aagttgctgc atccgctgtt tcaggccagc taatgcctga
    19621 ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata gcaaactgcg
    19681 tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc accgagggct
    19741 gagatatagc tggtaaacgt atggttaagg cacagcaagc gaaacgcggc ttcgcgaatt
    19801 tgcggggtaa cgttcggttc gctggacata tttgatacca ccgacgccag ttcagcatca
    19861 cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg cccctgatgg
    19921 tattgctcca gtatggcatc gagatagcga cagttggcct ctgtggcgcg ttcgagcatt
    19981 cgcggcagat tgcgaaactt ccagtcaggc cagatgtagc tcactgccgc ccacgcaatg
    20041 gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc ttcacccagt
    20101 aagttaaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgagc gtattgcacg
    20161 ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg cccttccagt
    20221 gatggcacaa accacagcac aggaatgcca atcgcgatac ctaccagcgt accaataatc
    20281 cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacgaa caaacttgtc
    20341 agcaagatcc aatacccgtg atgcattccg gttatttgaa tgatggcgta gccgaagcac
    20401 aacaccagcg acattcttac cgcatgacgg aagagggcgg attcaggcgt gaagtgacgg
    20461 ctaagacgca gccagatatc actcaatccg tgcgggctgt catcagcgag ctcattttcg
    20521 tcattattac ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag ttgggcatca
    20581 atggcgcgta aattgttcag caaaaatccc agtgttttga gtaaatcggc gggtgcgccg
    20641 ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc aaaatgcgga
    20701 tcatgttgat aagactgacg caacaaaata cagcgtgaca gttgctggca cgcctggccc
    20761 tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa atgttcacgc
    20821 aatgtttgat actgaatatg agaagagctg gcacgctcgt gaatatcctg tgcgacaaag
    20881 taataatgca gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa gcgggtcagc
    20941 agcgagagtt tcgtctgatt caatgtcgcc atcagctgac cgttggcgag agccaaatcg
    21001 tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga cttgagctca
    21061 agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg gcggaccggg
    21121 aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc cagcagatac
    21181 atcggctgct gataccagtg ctcatacagt gatgttccca acatagtgta aatggcgatc
    21241 agcaatgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag caaaatgaag
    21301 ccgctggtag agagcgttaa gccaatcgca aatagccagg gccagggaaa cagcaattct
    21361 actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg caaacgtccc
    21421 gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt tagcggaatc
    21481 gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag cgcaataaaa
    21541 atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa aggacttagc
    21601 ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc acgcgcagct
    21661 tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc atagtgcaat agcggcaatt
    21721 ttaaagttcg caatgccgac aggaatgcca atgattgaaa tacattgtgc gatgcccgtt
    21781 gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat attcaatacc
    21841 gtaccgccag tattcagcaa cacatttttg ccagccgggt tcagttcatc gacatgaata
    21901 gcttcattgc cataaggcac cagagacagt ttagtgatct cccagcagga tcgtgtcagc
    21961 ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc cagagtggtg
    22021 gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa acctcgcttt
    22081 actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga gcacgttttc
    22141 tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca tggaactgaa
    22201 agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg tgatcctcaa
    22261 tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca atcaattact
    22321 ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac tgccggacga
    22381 aaaagtggtg cgtctgcacg gcacagaatg gggcgagacg cagcgttttt accatcatct
    22441 tgatgctcac tggcggcggt ggagtggcga gatgagcgaa attgcgtctg gtgttttacg
    22501 ccagcaactg gatttgattg ccacgcgcac tggagaaaat aaatggctga cgcgtgagca
    22561 aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt tgccggttaa
    22621 ccgactggaa gaattcgata actgccgtga ggcgtggcgt aaatgtcagg cttggttgaa
    22681 agatattgaa agtgctcggt tgcagcataa ccaggcgtat accgaagcca tgcttaccga
    22741 gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc aggcccgggc
    22801 agtcgttaat ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg gaaaaacgtc
    22861 ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc ctgagcaaat
    22921 tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga ttcgcgaacg
    22981 gctacatacc gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc atattattca
    23041 gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg ctgcccgtca
    23101 tgaactcttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc aggcgaaggg
    23161 ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta acttctggga
    23221 tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa gtctgatgcg
    23281 gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagaga ttcgcgatct
    23341 gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag gtgcgctgaa
    23401 ggcagaaaac gctgtcgatt tttcgggcct tattcatcag gcgattgtga ttctggagaa
    23461 aggccgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg atatctcgcc
    23521 gcagcgcgca gcgttgttag cggcattacg caagcaaaac agtcagacga cgttgttcgc
    23581 cgttggtgat gactggcagg cgatttaccg attcagcggt gcgcaaatgt cgctcaccac
    23641 cgctttccat gaaaactttg gtgaaggcga tcgctgtgat ttagacacga cttaccgttt
    23701 taacagtcgt atcggtgagg tggcaaaccg gtttattcag cagaacccag gccagctgaa
    23761 aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat tggatgagag
    23821 tcaacttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag agcgcattct
    23881 gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg caacacgctg
    23941 gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac aggcggatta
    24001 cgtcatcatc gttggcttgc aggaggggag tgatggtttt ccggctgcgg cgcgggagtc
    24061 gattatggaa gaggcgctac tgccaccggt tgaggatttc ccggacgctg aagaacggcg
    24121 tttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt ttaacaaaga
    24181 gaatccctct ccctttgtgg aaatactgaa aaatctggat gtgccggtgg cgagaaaacc
    24241 gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc agcgagataa
    24301 cgctgataat cggggataag aatatcgacc gcgtcgttga aatgcggcga ctggataatg
    24361 aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc cagacgcagc
    24421 aaggctttca cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa gaaaatcaat
    24481 acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc cattgggcca
    24541 ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa tcaagttacc ggtagtgcct
    24601 gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca gctcatcagc
    24661 atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgtagg taaagtgcga
    24721 gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact gtaagcactt
    24781 atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct caggaagatg
    24841 gttgtttttc cgcccattgc aggaaagtat ttcgcgtttg tgggtcagcc agtttaaacc
    24901 aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca tccagctcgg
    24961 aaacacccga aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc ggtagtgaag
    25021 cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc gtttttgccg
    25081 ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg ccatccaaca
    25141 attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc aggcgaggca
    25201 ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc ggtggggaga
    25261 tatacagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga aacactaact
    25321 gatgcggtcc attgtccagt tcaatgctgt ccgcaccgcg cagcagagag ctggaaacct
    25381 tcttgccgtc gagtaccaga agatcgacat cggttgaaag ccgcagcgtg gtggcaaaaa
    25441 cggataccgg caaacataat gcaatcaagg tggtcacgat gccggttttc ataggtagct
    25501 cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc atacttacta
    25561 acatatagac atatttcccg cttttgctct cattcattcg tattagctgc atggttggca
    25621 tgtcggcttt gtcgtatact ttgttaaaca gtctggagga aataatgaaa gaaaccgata
    25681 ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc gacaaacccg
    25741 atcgccccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac gtcattccgg
    25801 tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc acgctggcag
    25861 acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg gcgtggggcg
    25921 tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg ggggtgatta
    25981 atgagcaagc ggcagtactg gcacgggatg ccgggttaaa tgtggtgatg gatcgctgcc
    26041 cggctattga gatccctcgc ctgggtctgg ccaaataaaa aatccccgga aggcaaaaac
    26101 cttccgggga tttgttcagg gattagttac gcagacgcgg ggcctggagt tgtttgcgga
    26161 tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc aattcgctac
    26221 ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc tccatcacca
    26281 cgtgatacca cggagcggcg cgaagctcgt cattcaccgc cagctcatca ggcgacggtt
    26341 cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct aacagggaat
    26401 ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc cgggaaatct
    26461 ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga caggcaaacc
    26521 ctttcagata tagcccttcc gggtaggtag cgatcaccgg atgatcggct gcctgacgga
    26581 actgctctat aaattgtaca tcacgaccgg catcaattgc ggcatccgcg atgattttct
    26641 gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata ccgccttcat
    26701 tcagcaactg aatcgccagc atgttgatgt ctttataacc acgacacgcg cccatcaact
    26761 ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat ttttcaccgc
    26821 gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca gccttgctca
    26881 gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc tgggaggtat
    26941 caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa ccaccggtat
    27001 aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc aggcggctgt
    27061 ctcgctggtc cagatagtag cccgttttgt gtccgtgctg aatatccacc agcagcttca
    27121 tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg aggccctggg
    27181 tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa atcgcacatt
    27241 ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct gcgccagcac
    27301 tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc ggcaggccat
    27361 cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc agccagtcac
    27421 gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata gactcagacg
    27481 ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg ccgcgtgcta
    27541 accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct ttaccttcca
    27601 tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat ttttcgcgcc
    27661 ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca aagaaatggg
    27721 cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata aggagagcag
    27781 caatgtcgaa agtctgcata attgcctgga tttacgggcg agttcagggc gtaggatttc
    27841 gctacaccac acagtacgaa gcgaaaagac tgggcttaac cgggtacgcc aaaaatcttg
    27901 acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtagaa aaattaatgc
    27961 agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc agcgagccgc
    28021 atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt accggttttg
    28081 gcaggccagc aattttggtg gcttgcttag ccgggccttt cgggaacagt cggtataaat
    28141 agcggctgtt acctttttct tcaccaaatt tattcgccat cgcttttacc agcatacgaa
    28201 tcgccggaga cgtattaaat tccaggtaga agtcacgtac aaaacgcacc acttcccagt
    28261 gttctggcga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc ggttcactcc
    28321 actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta ccttcgaaga
    28381 tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa acaaagcccc
    28441 gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg gcgaagccca
    28501 gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta accgtggcac
    28561 gaatatagtt cgtttcaccg ccatgaatga tgttgctggt ttcaaacaag atagcgccag
    28621 aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagt tgcaggaaga
    28681 tattagcaac cataccaatc agtaccacca caatacccgc catcagcata ccgccgagga
    28741 acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac actaacgccg
    28801 ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag gtgttcagaa
    28861 ttggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata atcccggtcg
    28921 gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc agcgtcagaa
    28981 tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc gccgaaaagg
    29041 ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg ctaagcagtg
    29101 atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct atgacagatg
    29161 taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt acccatcttt
    29221 acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc aaatataacg
    29281 ccctgagaat tccgacaggc aaaagaaaaa ggggttagca tttagctaac cccttatctt
    29341 atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg gttttcaaga
    29401 ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa acatcccgat
    29461 gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa tagccaaaag tgcattgata
    29521 gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct tttactcgtt
    29581 tagcaaccgg ctaaacatcc ccatcgcccg ggcaaagaaa aataggtcca tttttatcgc
    29641 taaaagataa atccacacag tttgtattgt tttgtgcaaa agtttcacta cgctttatta
    29701 acaatacttt ctggcgacgt gtgccagtgc agaaggatga gctttcgttt tcagcatctc
    29761 acgtgaagcg atggtttgcc ttgctacagg gacgtcgctt gccgaccata agcgcccggt
    29821 gtcctgccgg tgttgctagg agaagagacg tgcgatatga ataacgagga aacattttac
    29881 caggccatgc ggcgtcaggg cgttacccgg cgcagctttc tcaaatattg tagtctggct
    29941 gccacgtcgc tgggattagg cgcgggaatg gcaccaaaga ttgcctgggc gctggagaac
    30001 aaaccgcgca ttccggtggt atggatccac ggtctggaat gtacctgctg taccgaatct
    30061 tttatccgct ccgctcaccc actggcgaag gacgtcatcc tttccctgat ttccctcgat
    30121 tacgacgata ccctgatggc tgccgccgga acccaggcgg aagaagtctt tgaagacatc
    30181 atcacgcaat ataatggcaa atatatcctc gcagtagaag gtaatccgcc gctgggcgag
    30241 caggggatgt tctgtatcag cagcggtcga ccgtttattg agaaactcaa acgtgccgct
    30301 gccggagcca gcgcgattat cgcctgggga acctgcgcgt cctggggctg cgtgcaggcc
    30361 gcgcgaccta atccgacaca ggcaacgcct atcgacaaag tcatcaccga caaacccatt
    30421 atcaaagtac ctggctgccc gccgatcccg gatgtgatga gcgccatcat tacttacatg
    30481 gtgacctttg atcgcttgcc agatgtcgac agaatgggtc gtccactgat gttttatggt
    30541 cagcgtatcc acgataaatg ctatcgccgc gctcacttcg acgccggaga gttcgtccag
    30601 agttgggatg atgacgctgc ccgcaaaggt tattgcctgt acaaaatggg ctgcaaaggg
    30661 cctaccacct ataacgcctg ttcctccaca cgctggaatg atggcgtttc tttcccaatc
    30721 cagtctggtc acggctgcct gggctgtgcg gaaaatggtt tctgggatcg cggttcgttc
    30781 tacagccgcg tggtcgatat tccgcaaatg ggtactcatt ccaccgccga taccgtcggt
    30841 ttaaccgcgc ttggcgtggt ggcagcagct gttggtgtgc acgcagttgc cagcgccgtt
    30901 gaccaacgca gacgtcataa ccagcaacct acagaaaccg aacatcagcc aggcaatgag
    30961 gataaacagg catgagcact cagtacgaaa ctcagggata caccatcaat aatgccggac
    31021 gccgcctggt ggtcgacccg attacgcgca tcgaaggcca catgcgctgc gaagtgaata
    31081 ttaacgatca gaatgtgatc accaatgccg tctcctgcgg caccatgttt cgcgggctgg
    31141 agatcatcct gcaagggcgc gacccgcgcg atgcgtgggc gttcgttgaa cgtatctgcg
    31201 gcgtctgtac tggcgtacac gccctggctt cggtttacgc catcgaagat gccatcggta
    31261 ttaaagtgcc ggacaacgcc aatatcatcc gcaacattat gctggcaacg ctctggtgcc
    31321 acgatcatct ggtgcacttc tatcagcttg ccgggatgga ctggatcgac gtgttagatg
    31381 cgctgaaagc cgacccgcga aaaacctccg aactggcgca aagtctctcc tcttggccga
    31441 aatcatcccc tggctatttc ttcgacgtac aaaatcgcct gaagaaattc gttgaaggcg
    31501 ggcagttggg gatcttccgc aatggctact gggggcaccc gcagtacaaa ctgccgccag
    31561 aagccaacct gatgggcttt gcccactatc tcgaagctct cgatttccaa cgtgaaattg
    31621 tcaaaattca cgcggtcttt ggcggtaaaa acccgcatcc aaactggatt gtcggcggga
    31681 tgccttgcgc cattaacatt gacgaaagcg gcgcggtcgg ggcggtcaat atggaacgcc
    31741 tgaacctggt gcagtcgatt atcacccgca cggcagactt cattaacaac gtgatgatcc
    31801 ccgacgcctt agtcatcgtt cagttcaaca aaccgtggag cgaaatcggc acgggtcttt
    31861 ctgataaatg tgtcctcagc tacggcgcat tcccggatat tgccaacgac tttggtgaga
    31921 aaagtctgct gatgcctggc ggcgcggtga ttaacggcga cttcaacaat gtgctgccag
    31981 tggatttggt tgatccgcag caggtgcagg agtttgtcga tcacgcctgg tatcgttatc
    32041 ccaacgatca ggtcgggcgt catccattcg atggcattac cgacccgtgg tacaaccccg
    32101 gcgatgtcaa aggcagcgat accaacattc agcagctgaa tgaacaggaa cgctactcgt
    32161 ggatcaaagc gccacgctgg cgcggtaacg cgatggaagt ggggccgctg gcacgcacgt
    32221 taatcgctta tcacaaaggc gatgctgcga ccgttgagtc ggtcgatcgc atgatgtcgg
    32281 cgttgaacct gccgctttcc ggtatccagt caacgttggg ccgcattttg tgccgcgcgc
    32341 acgaagcgca gtgggccgca ggtaagttgc agtatttctt cgacaagctg atgaccaacc
    32401 tgaaaaacgg caatctcgcc actgcttcca ctgaaaaatg ggaacctgca acctggccga
    32461 cagagtgccg tggcgtcggc tttaccgaag cgccgcgcgg agcgttaggc cactgggccg
    32521 ccattcgcga tggcaagatt gatctctacc agtgcgtggt gccgaccact tggaacgcca
    32581 gcccgcgcga tcccaaaggg cagattggcg cttatgaagc ggcgctgatg aacaccaaaa
    32641 tggcgatccc cgagcaaccg ctggagatcc tgcgtactct gcacagcttt gatccgtgcc
    32701 tcgcctgttc aactcatgtg ctgggcgacg acggtagcga gctgatctcc gtgcaggtgc
    32761 gttaacagcg aaggagaatc atcatgcaac agaaaagcga caacgttgtc agccactatg
    32821 tctttgaagc accagttcgt atctggcact ggctgacggt gttatgcatg gcggtgttga
    32881 tggtcaccgg ttactttatc ggcaagccgc taccttccgt cagtggcgag gcgacgtatc
    32941 tgttctatat gggttacatc aggttaattc acttcagcgc cgggatgatt tttaccgtgg
    33001 tcttgctgat gcggatctac tgggcttttg ttggcaatcg ttactcccgc gagctgttta
    33061 tcgtgccggt atggcgtaaa agctggtggc agggcgtgtg gtatgaaatc cgctggtatc
    33121 tgtttctggc aaaacgtccg agtgccgata taggccataa ccccatcgcc caggcggcga
    33181 tgttcggcta tttcctgatg tcggtcttta tgatcatcac cggttttgcg ctgtacagcg
    33241 aacacagcca gtacgctatt tttgcgccgt tccgttatgt ggtggaattt ttctactgga
    33301 cgggtggcaa ctcaatggac attcacagct ggcatcggct ggggatgtgg ctgattgggg
    33361 cgtttgtgat cggtcatgtc tacatggcgc tgcgtgaaga catcatgtcc gacgacacgg
    33421 tgatctccac catggtcaac ggctaccgta gccacaaatt tggcaaaata agtaacaagg
    33481 agcgttcatg agcgagcaac gcgtggtggt catggggctg ggcaacctgc tgtgggccga
    33541 tgaaggcttc ggcgtgcggg tggcggaacg gctgtatgcc cattaccact ggcccgagta
    33601 tgtggagatt gtcgatggcg gtactcaggg actgaacttg ctggggtatg tcgaaagcgc
    33661 cagccatctg ttgattctcg atgccattga ctacgggctg gaacctggaa cgctgcgaac
    33721 ctatgccgga gaacgcattc cggcctatct cagcgcgaag aaaatgagcc tgcatcagaa
    33781 cagtttctcc gaagtgctgg cgctggcaga tatccgcgga catctgccag cacatattgc
    33841 cctcgtcggg ctgcaacccg cgatgctcga cgactacggc ggcagcctga gcgaactggc
    33901 acgggagcaa ctgcccgctg cggaacaatc ggcgctggcg cagttggctg cgtggggaag
    33961 tgtgccgcaa ccggctaatg aatcgcgttg tctcaattat gactgtctgt cgatggaaaa
    34021 ttacgaaggc gttcgcttgc gccagtaccg gatgacacag gaggagcagg gatgagcaac
    34081 gacacgccat ttgatgcgtt gtggcaacga atgctggcgc gcggctggac gccggtcagt
    34141 gaatcccgtc ttgacgactg gcttacgcaa gtgccagacg gcgtggtgtt attaagcagt
    34201 gacccgaaac gcacgccaga ggtcagcgat aatccggtaa tgattggcga attactgcac
    34261 gagtttcccg actatacatg gcaggtggcg attgctgacc ttgagcagag cgaagccatc
    34321 ggcgatcgct ttggcgcctt tcgctttcct gccactttag tgtttaccgg cggaaactat
    34381 cgcggcgtgc tgaatggtat tcacccgtgg gcggaactga taaacctgat gcgcgggctt
    34441 gtcgaaccgc agcaggagcg tgcctcatga gcgaaacttt tttccatctg ctggggccag
    34501 gaacgcaacc gaacgatgac agtttcagca tgaatccact gccgatcacc tgtcaggtga
    34561 atgatgaacc gagtatggcg gccttggagc aatgtgctca cagcccgcag gtgattgcgc
    34621 tgttaaacga gttacaacat caactaagcg aacgccagcc gccgttgggc gaggtgctgg
    34681 cagtcgatct gttaaatctc aacgccgacg atcgtcactt tatcaatacg cttctcgggg
    34741 aaggggaagt gtcagtgcgc attcagcagg ctgacgacag taaaagtgaa atacaggagg
    34801 cgatcttctg cggattatgg cgggtgcgca gacgtcgcgg cgaaaagttg ctggaggaca
    34861 aactggaggc tggctgcgcg ccgctggcgt tgtggcaggc ggcaacgcaa aatctcttgc
    34921 cgacagattc gctgttaccg ccgcccattg atggcctgat gaatggccta ccgttggcgc
    34981 atgagttact ggcacatgta cgtaaccccg acgcgcagcc gcacagcatt aatctgacgc
    35041 aattacccat cagcgaggct gatcggcttt ttctctcacg tctctgtggg ccgggaaata
    35101 ttcagattcg taccattggc tatggcgaga gctatatcaa cgccacgggg ttacgccatg
    35161 tctggcattt acgctgtacg gacaccttaa aaggcccgtt actggaaagt tatgaaatct
    35221 gcccaatacc ggaagtggtg ctggcagcgc cagaagattt ggtcgactct gcgcagcggc
    35281 ttagcgaggt atgtcagtgg ctggcggaag ctgcaccgac gtaaaaagac ggtaagtatc
    35341 gctttcagtc ttatgaatat cgcaatcggc gaatacctct ggtcgtagag tttcaggata
    35401 aagagggaga tctaacatta tcgggttatt tttctctctt cgcctacagg agtgcgcatg
    35461 tgggatgtca ttgatttatc gcgctggcag tttgctctga ccgcgctgta tcacttttta
    35521 tttgtacccc ttaccttggg gctgattttt ttgctggcta tcatggaaac catttacgtg
    35581 gtcaccggca aaacaatcta ccgcgatatg acgcgcttct ggggtaagct cttcggtatc
    35641 aattttgctc ttggcgtggc taccggcctg accatggagt ttcagtttgg tactaactgg
    35701 tcattctatt ccaactatgt gggcgatatt tttggcgcac cactggcgat ggaagcatta
    35761 atggccttct tcctcgaatc cacctttgtc gggctgttct tcttcggctg gcaacggctg
    35821 aataaatacc agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgca
    35881 ttgtggatat tgaatgccaa cggttggatg caatacccga ccggtgcgca ttttgatatc
    35941 gacaccctgc gtatggagat gaccagcttc agcgagctgg tctttaaccc ggtcagccag
    36001 gtgaaatttg tgcacaccgt catggcgggc tacgtgaccg gggccatgtt tattatggcg
    36061 atcagcgcct ggtatttact gcgcggacgg gagcgcgatg tcgcattacg ctcgtttgcc
    36121 atcggttccg tcttcggtac tctggcgatt atcggtaccc tgcaactcgg agacagttct
    36181 gcgtatgaag tcgcgcaagt acaaccggta aaactggcgg cgatggaagg ggagtggcaa
    36241 acggaacctg ctcctgcacc gttccatgtg gttgcctggc cggaacagga tcaagagcgt
    36301 aacgcctttg ccatcaaaat tcccgcgctg ctagggatcc tcgccactca ctcattagat
    36361 aaacccgtgc cgggtctgaa gaatttgatg gctgaaacct acccacgctt gcaacgcgga
    36421 cgtatggcct ggctgttaat gcaggaaata tctcaaggca atcgtgagcc gcatgtgttg
    36481 caggcattcc gggagctgga aggcgacctt ggttacggca tgttgctctc ccgctatgcg
    36541 ccggatatga atcatgtcac agccgcacag taccaggcgg cgatgcgtgg cgcgatacct
    36601 caggttgcgc cggtattctg gagtttccgc atcatggtgg gctgtggttc cctgctgcta
    36661 ctggtgatgc tgattgcgct tgtccagacg ctgcgtggca aaatcgacca gcatcgctgg
    36721 gtgctgaaaa tggcgctctg gagtttgccg ttgccgtgga ttgcgattga agccgggtgg
    36781 tttatgaccg agtttggtcg tcagccgtgg gcgatacagg acatcttacc gacatactcc
    36841 gcgcactccg ctttaaccac aggacaactg gctttctcac tgatcatgat cgtagggctt
    36901 tacaccctgt tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg tctggggccg
    36961 agcgcgatgc agagtgaaca accgacgcag caacaggggt aaaggagaaa atcatgtttg
    37021 attatgaaac attgcgcttc atctggtggc tgctgattgg cgtgatcctg gtggtcttta
    37081 tgatctccga cggatttgac atggggatcg gctgtttgct gccgctggtg gcgagtaatg
    37141 atgatgaacg ccggatagtg attaatagcg ttggtgcgca ctgggaaggc aatcaggtct
    37201 ggttgatcct cgcaggtggg gcattatttg ccgcctggcc cagagtgtat gcagcggcgt
    37261 tttccggctt ttatgtggcg atgatcctgg tgctgtgctc gctgttcttc cgtccgctgg
    37321 cctttgatta tcgcggaaaa attgccgatg cacgctggcg taaaatgtgg gatgccggtc
    37381 tggtcatcgg cagtctggtg ccgccggtag tcttcggtat cgccttcggc aacttgttgc
    37441 tcggcgtgcc gtttgccttc acaccgcaat tacgcgtgga gtatctcggc agcttctggc
    37501 aactgctgac gccattccct ttattgtgtg gtttgctcag ccttgggatg gtgattttgc
    37561 aaggtggcgt ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg
    37621 cgactaaacg cgctgcactg ttggtgatgc tgtgcttttt gctggcaggt tactggctgt
    37681 gggtcggtat tgatggcttt gtactgctcg cccaggatgc taacggtcct tccaatccgt
    37741 taatgaaact ggtggcagtg ctacctggtg cctggatgaa taatttcgtc gaatcgcccg
    37801 ttttgtggat cttcccgctg ctggggttct tctgcccatt gctgacggtg atggcgattt
    37861 atcgtggtcg cccgggttgg ggatttttga tggcatcatt gatgcaattt ggcgtgattt
    37921 tcacggcagg catcacgctg ttcccctttg tcatgccgtc aagtgtgagt ccaatctcca
    37981 gcctgacgtt gtgggacagc acttccagtc agctgacgct gagcattatg ttggtaatcg
    38041 tgctgatatt tttgcccatt gtgttgctct acactctctg gagctactac aaaatgtggg
    38101 gacgcatgac aacagaaact ctccgccgta acgaaaacga gttgtactaa ggagcaggaa
    38161 catgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt
    38221 gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt
    38281 agcatcgcat caggcaatca ataatgttag atatgaaaag cggaaacata tcgatgaaag
    38341 cgatcttaat cccattttta tctcttctga ttccgttaac cccgcaatct gcattcgctc
    38401 agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg
    38461 ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg ccaacctggc
    38521 cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat ctcggacatt
    38581 accaacgcca gcgtctggta gccgacggat tgctggcgaa aaagggctgc ccgcagtctg
    38641 ctcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc gaagccttcg
    38701 ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc
    38761 ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga
    38821 ctgacgcgat cctctgcagg gcaggagggt caattgctga ctttaccggg catcggcaaa
    38881 cggcgtttcg cgaactggaa cgggtgctta atttcccgca atcaaacttg tgccttaacc
    38941 gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga
    39001 gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat
    39061 ttctcctgca acaggcacaa ggaatgccgg agccggggtg gggaaggatc accgattcac
    39121 accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttacta caacgcacgc
    39181 cggaggttgc ccgcagtcgc gccaccccgt tattggattt gatcatggca gcgttgacgc
    39241 cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagtg ctgtttattg
    39301 ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc
    39361 caggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc
    39421 taagcgataa cagccagtgg attcaggttt cgctggtctt ccaggcttta cagcagatgc
    39481 gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag
    39541 gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg caaatcgtga
    39601 atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt cagttacctg
    39661 aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg aaaaggcgtt
    39721 cacgccgcat ccggtcactt tcagttttac tctttctcgg agtaactata accgtaatag
    39781 ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag gattgcgcct
    39841 ttaatattaa cgccagcctg ttccagacgc tgcattgaca agctcacctc tttggcggtg
    39901 ttcaagccaa aacgcgcaac cagcaggctg gtgccgacag agcgtcccac gaccgcagca
    39961 tcgctcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg gtcgttcgcc
    40021 cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt aggtggcacc
    40081 tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac tttgttgagc
    40141 tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt aaacaggtta
    40201 tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc ggactgggcg
    40261 atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt cgcaccggtg
    40321 atcatcagaa tgttattctc agtctccatc atagcgaaat gcagactggt tcgtagcgca
    40381 cgtacggctt ccacagcaga atccgccggg ttatccactg ccaggaaggg gatattttta
    40441 gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt gcgtttatcc
    40501 agccactcgg acattgggat agtggaataa acgctgatgc cgtgctcttc cagttgttcc
    40561 ggggcttcta caccacgacg caacatcgca cgcgccagca cggcactcac cgaaataaac
    40621 aggccaagaa taaaaccaag caccacattc aacgcttttt tcggtttcac tggctgcggc
    40681 tgagtgactg ccgggtcgat aatccgcacg ttaccaatgg cactggattt cgaaatactc
    40741 aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc tacgtcacga
    40801 cttaaacgca acacttcctg ttgggtggaa ggcattgccg atacccgctt attcaggcgt
    40861 ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt tgggtgatct
    40921 ttcttataca gcagggagat ctctgcctcg cggaaggtca gctcattgag ttgattatca
    40981 acgttcacaa tctgctcaag aacggctttg gcttccaggt taaggtcaac cgaatcgcgc
    41041 tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg cacttcaggt
    41101 aactggctct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg agcgatattc
    41161 tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg gggatcatca
    41221 ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac ggtaaaggtt
    41281 tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa ctgtgttcct
    41341 ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag acgctggccg
    41401 accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt ttccccaacc
    41461 gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca gctgatcgcc
    41521 agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat cggaaaatac
    41581 ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag aatcatgcgc
    41641 gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat catatcgctc
    41701 aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt atctgcctga
    41761 taaattggtg tgcttaacag cgatcagcgt gaataacgcg gtcacgctga taataaactt
    41821 acggtgatcc cataactcgc cgaccagacg aagcagatcg atctcatttt cctgagtgct
    41881 gcctggtggc gtattcatat ttttagttgt catcgtattt tataaactca aattaccggc
    41941 tgaggcgctt cgcccattcc tgactggcgc gctccaacat accgtagaca tgttcaaatg
    42001 cgtcctgact tttacgatag ggatccggga tctctttctg ttccagccat tgcccaaaca
    42061 gcattgtttt cccgcggacc tcgggagcaa ttgcggtaac ctgagcaata tgttccgact
    42121 ccattgccag aatcagatcg taatttcgtg ccatctcagc agtgagctta cgtccggcat
    42181 gtccttcaag agatacgcca tgattagcgg caacatctgc cgctgtcgca tctgcagggt
    42241 gttttaccag accatgaaca cctgccgatt tgacttttac gcccggcaga cgtttacgca
    42301 gcaagcgctc gccaatgggt gaacggcaaa tgttgccggt acaaaccact aggattgagt
    42361 taaattttag ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt catgcacccc
    42421 ggaaatcgtc ggtaccagca gagaaatcac acggttccaa cgagccagcg gcgcggtagt
    42481 gacataaaca atgtcgtaag gttcaagctg gaattccgtt ccgagtatca ttgccgatgc
    42541 gtccttcgca ttcagctgat aaatattggc gattttgcca ttctgcttgt tttgcgttgc
    42601 acgaataaca aaaatccccg tcgcatcagc gacatcctga ttcataccct cagcgttacc
    42661 cagcgcttcc gccagggtca taccgctgcg gtccattttc agcgtgctct gtttacccac
    42721 ttcacccatc acgaagactt tcagatcatc gttgcgcgga ataaataaaa tatcgcccgg
    42781 gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc agcgcataga ggttcacttt
    42841 ggtctttacg ccattctggg tgagcaccac gttgcgccag tctgcatctg cggtcagacc
    42901 gcccgcggcg ttaatcgcat ccatgatggt cagaggaata ttggtaatcg gctgctgacc
    42961 cgatttgctg acttcgccgg tgacgtacgc tttttgcgat cggaacgccg cgacgctgac
    43021 atcgacttgc gggctttcaa tgactgaatc gagacgcgct gtaatctcgt tgcgtacctg
    43081 ggtaagtgtt ttcccggcca ctttcaggcg accaatatag gggtagaaaa tcgcaccatc
    43141 ggcgttaacc cagttgccgg tatcactggc gctacggtat tgccctgcgg gggtggttag
    43201 ttccgggtga tcccagaccg taaccatcaa cacatcacca atcccgatac gatattcata
    43261 gtttgcgatt tgctgctcca gctccgggtt agcctgcgac ataatcggtt tggcgcgcag
    43321 ctgatcaatc aatcctggcg tgacgggata gatattgacc attttgtcca ggtcatactg
    43381 attgtcaggt agttcaatga catccttgtt actggtcgat aaattctgac caggaacaag
    43441 ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa aaatgttctt
    43501 tttcattggt ttatatttat cagtcaatgt aatgttatga attacgtcag gttattgtcc
    43561 aaccggtact tctcgctctg ccgtcattgg atcgagctgg tatttgcgtg acagcatttg
    43621 tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt taggtccaat
    43681 ggtcatcaag tcgaacggaa tcgagatata aaaaccttta ctaaagccgc cttcgccgta
    43741 gtcatcttta gatacgttac tgattgccgc ccatacccct accgccacgc cgctgtcaaa
    43801 gcgtttggcg acgtcgatcg ttgcgccttt atcttttgcc agatattgcc caacgctaag
    43861 tttcatcagt acgccattga gcgtcggcgg gttccagtaa gccgtcacga aacctgtggg
    43921 cgtggaataa tcggtgaagc gcatcatgtt gtcccagtca cgctgcttca cgtagttaac
    43981 gtccacaccc agcgcccagc tggcatctag cgggcgataa agaagctcgg aaccgacacc
    44041 ggcatacatc gtttccagat aaccgccata cacctggcca tagaaaccat tgcctaagtc
    44101 ggcaaagtaa ttcgcttgca agttgttgag ataaacgtca ttgcgaacgt aatcacggat
    44161 atgcgtgcgc acgcggggca gggtagagtc cgcgggtaac agtgaagact taaacttgtc
    44221 gtagttgttg taaatattgg tgaaaatacc gccatcaagc agcaggtggt cggtaaacca
    44281 gtagcgggcg ctggacatca gccccagctg gaacatatag aaatcttccg ggccgcccag
    44341 cgactgtgaa agtgttgggt tgaaagagta gctaaagcga tcttcacgaa tacggtagcc
    44401 ccgaccaaag gcagaaagat cttctgcttc aacacgttgt tgttgcagtt gctctgattt
    44461 accaggcgct gtgccggcca gctgtttgcg caggctggct acgtcggttt cggtagtcac
    44521 catcgccata tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt gcggcaggtt
    44581 attcaccaga atccggttgg cacgatcgac cgcttcgcga gagtcacggt atttgtattg
    44641 ctgaccagac atatacagcg tcttatcgcg tagctgaatt tctggcgcgt caaagcccgc
    44701 gttatacttc agggcggtaa gttggtttgc taccgtggtg tactgcaaac cttcagactc
    44761 tggcgcaggc tgccaggccg gttttggcgt atcgcgtagg gcagggcgca gatcgttgaa
    44821 attggtccgt aaagtgaaac caaacatcaa cgtgtttccg cgttcataac tgaggttgag
    44881 gtctgcccag ctggcagcgc gataaactgc accgacgttg aaatggcttg cctgaggcag
    44941 tttgccagcg aaatcattct ggtaattgtt gccgtcgtat tcgagtttca gacgcagggg
    45001 attccacggc gtttgatact caatgccgcc aaagatggaa gccgggccac gaaagatatc
    45061 gctaaagctg atatcgcccg catcgtgaga ctctgcgcga tgacaatatt tatcgctcac
    45121 ccggcaaaac gggttggtaa tattgcccgc attaccggcg tagccccatg ccatcccgag
    45181 ggtgaaatca aatggccccg cttgcttgct ggccaccaga tattcaccgt caaacaaacc
    45241 cgtaccagca atatcacgtt taccaaacgc cacttgcggt agccagtaac cttcttccca
    45301 caggcgaagt ttaaaatcga atgatttgtc tttgtatgac tgatcgccgc tgaaatcttc
    45361 ccactggcta tatttgcgtg tgcgcacatc cgtataacga atggtgcctt ccagccatgg
    45421 gaacagcgcc acggaggtgg agtagaaccg gtattgatcg ttatcccggt agttgacgct
    45481 gaattcacct tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg tgccgccaaa
    45541 gtctgattgc gacgaaccca gcggatccgg gtaggttaat acttcagcat gacaggcact
    45601 ggagacggca atggccaggc agcttaaaag ataagaattc ttcttcatca gtcaggaaca
    45661 cgttgcgtta aaacagacac aatctggtca tttaaatcag catatttttc gggcagtaca
    45721 tgcgcagaaa agcccaacca aagctggctg ccaggcggtg gttccacatg tcttttattc
    45781 cacagggcaa caggggcgac gacagtctca ccctcaggcg taatcaccat aacgttattc
    45841 ttgtccgcgc ctgcaaggcg ggggtgatct tgcagataat cggtaacgct acggcctgcc
    45901 tgccagggca attgcccagc cccagaaacc gcacccagca gagtaatagt caccggacgc
    45961 tgtacggtat agagcgtata gtccccgacc aacgggggat tgctgttctc atccactcgt
    46021 acaaaatcag ggtccagttt gaccggtaag cgaccagtga tattgagatt gagtaactgc
    46081 tggcgcacgg atttaatcgt tgccgccaca tcgtcgtcag cttctgcttc ccaggaagca
    46141 agctgtgcca tgacatgttg gtagtcttta agcgctttag ctttcgccgc agagtcggtc
    46201 agcaaggcac caggccacca gaggcgatcg cgcagttgcg gttgtgtcac caactggaca
    46261 acattttcca cggggccaac ggaaagtgtc tgttgttcgc taggcagata aatagtcacc
    46321 gttccttgcg caaaggcatg gggtgtcatt acgtaaagta cgctggcaat gaaatacgac
    46381 tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc tacgggaata acccctgcgc
    46441 ctaacatttg ccgactttga cgaacctggc cagtagccga atcgacccag aatgtgtttt
    46501 gccactgttt ttctggcagc gtggaagtca cctcttcctg ccagacatta cacagcacat
    46561 tgtgacccgc aatattcagc gtctctaatc cagcaaagga aaaagtagaa cttagggtcg
    46621 cagagcgaaa atggttgtct tcactccaga gaatatcgcg cgtccagcgg ctgccatctt
    46681 ttattgccag cgcgttacgc agagggtccc gcccgggatt tgtcacttcc agcagattat
    46741 tattaagttt gacggtcttt aaaagacgtc cattgtgggt aaccagcatg gcgttatcct
    46801 gggataacca tttgctttgt tcttgttcta tatatcccag taccacaaag attcgttgcc
    46861 cttcattaag gcgtaaatac atcgtggaat aaggcaacgc ctggatctgc tgatccgcta
    46921 cagtgatatc ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata ctttgctggc
    46981 tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa aataagaggg cgcaccttta
    47041 atccttgaaa ggttaattaa aaaaatgcaa atctctaaag cccggtacga atatcgcaac
    47101 cgggtattat ttaacttgaa tttcgacgcg tttaagcaga attagcgcgt agtggacgtg
    47161 gtggttgagg tgttatgaga accgccatcg ccactgtcat ttgctgcaac tactgccaac
    47221 gtcgccacac caacagccgt tgccacaccg acaactgtcg ccgtgccgac agcgccagca
    47281 gccacgccac tagtggtagc cgcagtcgtg gttgcacctg tagtggtcgt tgtggtgcca
    47341 gtagtacccg tcgttgcctc ggtcccgttt gctgcttcag gggcagcaaa cgcagctggc
    47401 gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt gtttcataac ttttccttta
    47461 ttcatcgcat ggacaatacg tacgcttgag atcaggattg tctactttga gtataacatc
    47521 cttgtgaaat ctccctttcc tcataccgct agcactatat actgcgggtg ggaaaggcgc
    47581 gcagagggaa atataagatt gttaacgatt tccctctgga aatacgactt gtgagcataa
    47641 gataaatctt agaaaaatac gaattaaaga taagaaaatt gttacgaaag ctattaatta
    47701 ttgttggtaa tatagtttca agtggtacta taactggcta ttaaatggca tttataacta
    47761 ttaggtgcag agatattcgc ttaaatggat tagtttcttg gtggaaaagt aactgatgtt
    47821 ataatgtatg gtgttgacta ttgaactatt ccaataaaac cagtcagctt taaacaagca
    47881 gcgtcatatt aagagagata aacatttgcc gctgttggtc ctcgcaggcc atttacgcgg
    47941 caaaattcca cgcgtaatcc tggtataagc acttctgcgt cgcggggagt gaatgcggaa
    48001 atatggacct ggacttcttt acgaccgtcg gaggggataa tgaatccttt gccgcttttg
    48061 cgatcaaagg ttttgacaat tcctgtcatt ttacgggaca aacaaattcc ttactgaaaa
    48121 tactgcgctg cactatacgg ggttaataaa ataaagccag cgatatttaa gaccgccgga
    48181 cggctaaaat aaaatttgct taatctcaat tatcatgcct taatagctgc gtcggtttga
    48241 aagacagaca gcatacaaag tagtttacta aagcagttct cattatcagg cattatcccc
    48301 ttcttttgag tctctctcct gaacactaag tggtttctgt attaaagccc tgtttgccga
    48361 aaggcccaaa atgaaggaag taaaatatgt ctaataaaat gactggttta gtaaaatggt
    48421 ttaacgcaga taaaggtttt ggctttatca ctcctgatga tggcagcaaa gacgttttcg
    48481 tccatttcac cgccatccag agcaatgaat tccgcacgct gaacgaaaat cagaaagttg
    48541 aattttctat tgagcagggg caacgtggcc ccgcggcagc gaacgttgtt acgctctaag
    48601 gttgccgtta ttactcaaca tctccatttc cgctgtccat gttgtcatgg ttcacagtac
    48661 cgtacatcgg cattcgatgt gacggagcga aaccctttgg gcgctaagtg tattttttgt
    48721 aaatcgacta tgatcacctt tgataacgtc gcgctgcaaa tacgcactga ccatgcgccg
    48781 ctggatttca caaaataata tcaggctccc tcgtggagcc ttttttatat ctgccttatt
    48841 tttcttcaac gctgtatgta tagtaagcga taacctgttg attattgaat ctttcgggga
    48901 gatggcttat aacattcctt acctgaccag ggtaccggga accaacacct tactggcgtg
    48961 ttgctgtctt tcaagaccag acgaggttaa cagtgaatat tgaagagtta aaaaaacaag
    49021 ccgaaacgga aatcgccgac tttatcgcgc aaaaaatcgc cgagctgaac aagaatacag
    49081 ggaaagaagt ctctgaaatt cgcttcatcg cacgagaaaa aatgaccggg cttgaaagtt
    49141 atgatgtcaa aatcaaaata atgtgatttt gtgaacatca ccccgtgcga ggtgatgttc
    49201 cgcttgttgc taatttagtg accaatcatt ggcgcttgtg gaattaagcg tcggtacaat
    49261 tcctccggca ccgggctttg ccatactccc gcatacattg cgtaaccaat caccgcaaac
    49321 ataatcccca gaaccagtag cgtcattaac cagccagaca acgcaaaggc ttttttattt
    49381 gctgcaggtt tttgcagtga aaaggtcaat gttgaggcta ccggacatga ctctacgcaa
    49441 gtcatacagc cggtacattc cactgttcgt acctgaatta atttatctac cgggatccgt
    49501 gatgggcact tttttgcgca tttgccacaa tcgatacaac tttcggcatt gcgacgaatc
    49561 ttaaacggcg acaatagcga aaccacgccc atcagcgcgc catatgggca aagataacga
    49621 caccaggcat ggcgaataaa caggctggca atcagcaaaa cggtcacgct gattaatgtc
    49681 gcggtcccca tatgacgaaa gaaatcgagc attttaacgt ccgtcaccac gctatagggc
    49741 gacaacataa aatagtgaat cgcctgagcg ggcatcaata acgcgatata gagaaataaa
    49801 ctcaacagca aatacttcac gccgcgcaga ggaatatcca gccagcgggg aaggacacat
    49861 tgccgaccaa acagtttgtt accgagatcg ccgattaatt cagaaagcgt accaaccggg
    49921 cataaccatg agcaaaaggc ctttttgagt aatagactga tgacgataaa agcgaccaat
    49981 aacagcatcg cggcggcgtg gacggacggt aactgacctg ttgcaaggct atatttcaga
    50041 ttcatcagac cggcaatcgg tagccagcct tcgatacctc ccggtctggc gacaaatgtc
    50101 gtactacctg ccgtttcgta atagcgcacc caataccaga acgtgatggc aatataaata
    50161 ttcattgcca atagtaagaa ttgcgtcgct ttacgccagg tcgtggcatt gcgccagtca
    50221 ttccacggta atttgccgcc cgtcgtgcct ggccgctgct gccagcgggt tctttttttc
    50281 tctgccatga ttttgccagt ccgttaagtt gcataccaaa tgccactatt ctagttattc
    50341 ttaactggct gatattgatt caaatcgcgt tcaggtcttt cttattcaat catgcttcca
    50401 gagcggcaac actgcgtgta atttcttcgt gtggaagggg ggcagataat ggctgctgct
    50461 ccagttgtgc gcatagctga ctggcgctac gcattcccag actcgagcaa ctgcttttta
    50521 gctgatgcgc ggcacgcttt attttctcgc tgtcctggct ggcgcgggca atgtcgattt
    50581 catcgagaag cggcagggca tgttgtttaa ataatgccag ccattcgtgg atcttctccg
    50641 tccccattaa ctgagcgtct tcattgagtt gcgagacatc cagtggtaga tcgttattgg
    50701 cttgcagttg gagatagtgc gccagtaact gaccgagcac ttcacgcggc accggtttag
    50761 ggataatccc gcgaaatagt gaactggtac gctggcgcag cgtttcgtca atgacatggg
    50821 cgctaaagcc aatcaaaacc agcgacggat attgctgtgc cagttgccgg gcaagggtaa
    50881 tgccgtcgat atccggcaga tcaaaatcca ccagtgcggc agcaaacggt tcgctatttt
    50941 gcagtgtctc taaagcctgc gcggcattgc caatagcaac aacctgcgca ccactggtgt
    51001 tcagcatctc aacggtaatt cgctgggtta gcgggttatc ttcaattaac agcaaacgta
    51061 aaccgtcaag acgcaccgcc tgattggctg tttttggcac gggtgcagtg gcaacacgta
    51121 acggcaagcg taaacaaaaa cagcttccaa cctccggcgt gctggtggcg ctcagttcgc
    51181 cgcccatcgc c
//
LOCUS       DADKXM010000015        49238 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.015, whole genome shotgun sequence.
ACCESSION   DADKXM010000015 DADKXM010000000
VERSION     DADKXM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.015"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="JQR05_000879"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="JQR05_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY5323101.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="JQR05_000880"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="JQR05_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY5323102.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="JQR05_000881"
     CDS             complement(937..1227)
                     /locus_tag="JQR05_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY5323103.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1661..2371
                     /locus_tag="JQR05_000882"
     CDS             1661..2371
                     /locus_tag="JQR05_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY5323104.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYLAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JQR05_000883"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JQR05_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY5323105.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4158)
                     /locus_tag="JQR05_000884"
     CDS             complement(3499..4158)
                     /locus_tag="JQR05_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY5323106.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4311..6644
                     /locus_tag="JQR05_000885"
     CDS             4311..6644
                     /locus_tag="JQR05_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY5323107.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6613..7053)
                     /locus_tag="JQR05_000886"
     CDS             complement(6613..7053)
                     /locus_tag="JQR05_000886"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5323108.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7050..7613)
                     /gene="tag"
                     /locus_tag="JQR05_000887"
     CDS             complement(7050..7613)
                     /gene="tag"
                     /locus_tag="JQR05_000887"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY5323109.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7771..8469
                     /locus_tag="JQR05_000888"
     CDS             7771..8469
                     /locus_tag="JQR05_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5323110.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8698..9906
                     /locus_tag="JQR05_000889"
     CDS             8698..9906
                     /locus_tag="JQR05_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5323111.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10230..11921
                     /gene="eptB"
                     /locus_tag="JQR05_000890"
     CDS             10230..11921
                     /gene="eptB"
                     /locus_tag="JQR05_000890"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY5323112.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12013..12089
                     /locus_tag="JQR05_000891"
     tRNA            12013..12089
                     /locus_tag="JQR05_000891"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12047..12049,aa:Pro,seq:cgg)
     gene            12833..14440
                     /gene="dppA"
                     /locus_tag="JQR05_000892"
     CDS             12833..14440
                     /gene="dppA"
                     /locus_tag="JQR05_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY5323113.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14748..15767
                     /gene="dppB"
                     /locus_tag="JQR05_000893"
     CDS             14748..15767
                     /gene="dppB"
                     /locus_tag="JQR05_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY5323114.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15777..16679
                     /gene="dppC"
                     /locus_tag="JQR05_000894"
     CDS             15777..16679
                     /gene="dppC"
                     /locus_tag="JQR05_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY5323115.1"
                     /translation="MSQVTENKVISAPVPMAPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16690..17673
                     /gene="dppD"
                     /locus_tag="JQR05_000895"
     CDS             16690..17673
                     /gene="dppD"
                     /locus_tag="JQR05_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY5323116.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17670..18674
                     /gene="dppF"
                     /locus_tag="JQR05_000896"
     CDS             17670..18674
                     /gene="dppF"
                     /locus_tag="JQR05_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY5323117.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18704..19975)
                     /locus_tag="JQR05_000897"
     CDS             complement(18704..19975)
                     /locus_tag="JQR05_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5323118.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20451..20558
                     /locus_tag="JQR05_000898"
     CDS             20451..20558
                     /locus_tag="JQR05_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY5323119.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(20645..22324)
                     /gene="bcsG"
                     /locus_tag="JQR05_000899"
     CDS             complement(20645..22324)
                     /gene="bcsG"
                     /locus_tag="JQR05_000899"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY5323120.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22321..22512)
                     /gene="bcsF"
                     /locus_tag="JQR05_000900"
     CDS             complement(22321..22512)
                     /gene="bcsF"
                     /locus_tag="JQR05_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY5323121.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(22509..24080)
                     /gene="bcsE"
                     /locus_tag="JQR05_000901"
     CDS             complement(22509..24080)
                     /gene="bcsE"
                     /locus_tag="JQR05_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY5323122.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQAQTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            24353..24542
                     /locus_tag="JQR05_000902"
                     /pseudo
     CDS             24353..24542
                     /locus_tag="JQR05_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24554..25306
                     /gene="bcsQ"
                     /locus_tag="JQR05_000903"
     CDS             24554..25306
                     /gene="bcsQ"
                     /locus_tag="JQR05_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY5323123.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQINHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            25303..27911
                     /gene="bcsA"
                     /locus_tag="JQR05_000904"
                     /pseudo
     CDS             25303..27911
                     /gene="bcsA"
                     /locus_tag="JQR05_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            27922..30261
                     /gene="bcsB"
                     /locus_tag="JQR05_000905"
     CDS             27922..30261
                     /gene="bcsB"
                     /locus_tag="JQR05_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY5323124.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRGDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGSSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLSTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            30268..31374
                     /gene="bcsZ"
                     /locus_tag="JQR05_000906"
     CDS             30268..31374
                     /gene="bcsZ"
                     /locus_tag="JQR05_000906"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY5323125.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTGPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            31356..34829
                     /gene="bcsC"
                     /locus_tag="JQR05_000907"
                     /pseudo
     CDS             31356..34829
                     /gene="bcsC"
                     /locus_tag="JQR05_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            34911..36899
                     /gene="hmsP"
                     /locus_tag="JQR05_000908"
     CDS             34911..36899
                     /gene="hmsP"
                     /locus_tag="JQR05_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY5323126.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37083..38369
                     /locus_tag="JQR05_000909"
     CDS             37083..38369
                     /locus_tag="JQR05_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY5323127.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVEVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            38590..40086
                     /locus_tag="JQR05_000910"
     CDS             38590..40086
                     /locus_tag="JQR05_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY5323128.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40182..41330)
                     /locus_tag="JQR05_000911"
     CDS             complement(40182..41330)
                     /locus_tag="JQR05_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY5323129.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLHECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41343..42110
                     /gene="pdeH"
                     /locus_tag="JQR05_000912"
     CDS             41343..42110
                     /gene="pdeH"
                     /locus_tag="JQR05_000912"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY5323130.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQLLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42180..44240
                     /locus_tag="JQR05_000913"
     CDS             42180..44240
                     /locus_tag="JQR05_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY5323131.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLTPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKQRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVIGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44422..45744)
                     /locus_tag="JQR05_000914"
     CDS             complement(44422..45744)
                     /locus_tag="JQR05_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY5323132.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIATWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46155..47168)
                     /gene="yhjD"
                     /locus_tag="JQR05_000915"
     CDS             complement(46155..47168)
                     /gene="yhjD"
                     /locus_tag="JQR05_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY5323133.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47217..48188)
                     /locus_tag="JQR05_000916"
     CDS             complement(47217..48188)
                     /locus_tag="JQR05_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5323134.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            48636..49238
                     /locus_tag="JQR05_000917"
     CDS             48636..49238
                     /locus_tag="JQR05_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5323135.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALVVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11737 a        12334 c        13326 g        11841 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 gcaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccac atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacaacggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact accttgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcggc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4141 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4201 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4261 taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact
     4321 catcctcacg atattccgtt ctgactgccg cccactgggg gcccatgctg gttgaaaccg
     4381 acggcgaaac cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa aactccttgc
     4441 agagcgcggt tcgcgaccag gttcacagca atacgcgggt acgatttcca atggtgcgca
     4501 aaggctttct cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat gaatttgttc
     4561 gcgtgagttg ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg
     4621 cttatggtcc ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc
     4681 ataaggcctc gacattatta caacgctata tggcgctggc aggcggttat accgggcatc
     4741 tgggggatta ttcgaccggt gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg
     4801 aagtttatca acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt
     4861 tgtggagtgc taacccactc aatacgctga aaattgcgtg gaatgcatcc gatgagcagg
     4921 ggctttctta cttttccgca ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa
     4981 tgcgatcgga aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg
     5041 gcaccgatgt tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg
     5101 acgaagcgtt tctggcgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg
     5161 gcgagagtga cggaatagcg aaaaacgccg aatgggcggc agagatttgt ggtgttggcg
     5221 cagcgaaaat ccgcgagctg gcggctattt tccaccaaaa taccaccatg ctgatggcag
     5281 gctggggaat gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg
     5341 cagcaatgtt ggggcaaatc ggcacacccg gcggcggttt tggtctttct taccattttg
     5401 ccaatggtgg taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc
     5461 cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa gcactggaaa
     5521 accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat attcgtttta
     5581 tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt ctgatccgtg
     5641 cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac
     5701 acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg
     5761 gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag
     5821 cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag ggcggctatg
     5881 cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat aacgttgccc
     5941 ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca
     6001 accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt
     6061 tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct
     6121 cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc
     6181 cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg
     6241 cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtagcaa
     6301 accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata accgaagggg
     6361 atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc attagcgagg
     6421 gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg
     6481 ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc
     6541 ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc
     6601 cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg
     6661 ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat
     6721 cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata
     6781 ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc
     6841 cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac
     6901 ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta
     6961 attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc
     7021 ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag
     7081 ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg
     7141 ccgacaaact taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta
     7201 gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac
     7261 gaccagacaa agtcgggaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca
     7321 ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt
     7381 ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag
     7441 gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct
     7501 tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccactcatta
     7561 tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc catactttcc
     7621 ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag
     7681 tcaatattta atacagggct actatgcccg atatccttca tacaatgaca ataatattgc
     7741 cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg
     7801 cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc
     7861 tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg ttacacctgg
     7921 gatagtgatc atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa
     7981 agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt
     8041 atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca
     8101 ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg
     8161 ctgaccgatc cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg
     8221 cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc
     8281 tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg
     8341 gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta tgctgcgcta
     8401 actcaggcag aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc
     8461 agattttaac gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg
     8521 ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac
     8581 taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaaatgc
     8641 acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg
     8701 acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat cattacccag
     8761 tttgcgctgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc tgccaagctg
     8821 gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt
     8881 tcgtcttctg ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccgtggct
     8941 tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg
     9001 ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg
     9061 ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc gttcgctatc
     9121 ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct gctcgaaacg
     9181 gtgggtctgg aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat gattgttttc
     9241 ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg
     9301 gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta
     9361 atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc
     9421 caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt tatttccatc
     9481 gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc ccgtatccgt
     9541 gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca
     9601 ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact
     9661 attaccgtct ttccatcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac
     9721 tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat tatcgcctca
     9781 ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg
     9841 gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc
     9901 ctttaatgct cagccgcgag ctactgggct tcagcttccg tgtgcatcaa acgcatcaac
     9961 gaaatgtata aacaatgact cgtaaaattt gggccagtta actggccctt tttactgctt
    10021 tttgtaacgc taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca
    10081 ctctttcccc acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac
    10141 ttatctgact acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt
    10201 tatcacctgt ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga
    10261 agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc
    10321 gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg
    10381 aactggccgc caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc
    10441 gccgcagctg gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt
    10501 acatgacctt ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg
    10561 atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta
    10621 gtgcattgcc tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac
    10681 tgcgaacccc agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg
    10741 tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg
    10801 ttgatttgcc gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt
    10861 ctgcgttggg gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc
    10921 ttaatccggc gaagaagttt acataccagg ctccacaaaa cgttgatgat acttatgtcg
    10981 tgtttatcat cggtgaaacc acgcgttggg accatatggg tattttcggc tatgagcgta
    11041 ataccacgcc gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg
    11101 ataccgcaac caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata
    11161 atccgcagcg cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca
    11221 gttctgacct ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca
    11281 acattgctta tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg
    11341 atatgttgct ggtagatgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc
    11401 tgatcattct gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct
    11461 tcgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga
    11521 tcaactccta tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc
    11581 aggttcgcga taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg
    11641 aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggagcag ttccgcgtac
    11701 cgatgatggt ctggatgtca gataaatatc tggaaaatcc ggccaatgcg caggcgtttg
    11761 cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgaca
    11821 ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact
    11881 ggtgtcacat cccgcagaca aaagtggcag cggctaacta agcggcctgc tgactttctc
    11941 gccgatcaaa aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat
    12001 gcgccccgca ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt
    12061 cggaggttcg aatcctctat caccgaccaa attcgaaaag cctgctcaac gagcaggttt
    12121 ttttgcattt gtagctcatg aggatgagaa cctccggggg cagggaggct cgactcgacg
    12181 taggcctgat aagacgcgcc agcgtcgcat caggcaacgg caccgcactt attgtcggat
    12241 gcggcgcgac cgccttattc gaccaacaca ccacacattt tcctgccaac tttgcgcccc
    12301 atatctcact atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct
    12361 taaatctttt ttgcgttact tatggctggc gctggcattt tccgctgtgc gttttagcgt
    12421 tcatggtatt aatcgctcag atatccacct tttcctcaga tttttttcat taaatgcata
    12481 aaacttaatc accaaatgtt gctaaatatt aagctgattg ttctggttat gttggagtag
    12541 cacaaatttc tctgctgcaa atagctgttt gaagtttttt taatctttgt ttgtggattc
    12601 tcaccgttgg tgtaaatccc ggttggctgt attgacgttt tcacattctg ttgacagatt
    12661 gtaggtcacg aggggcattt tatggaggat ccgcactgtt acactgatgt taattagtac
    12721 ggcatcccca cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg
    12781 acaacccatt gcaaagggtt aaaacaacaa acatcacaat tggagcagaa taatgcgtat
    12841 ttccttgaaa aagtcaggga tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc
    12901 agcaagtgtt caggctaaaa ctctggttta ttgctcagaa ggatctccgg aagggtttaa
    12961 cccgcagctg tttacctccg gcaccaccta tgacgcctct tccgtcccgc tttataaccg
    13021 cctggttgaa tttaaaatcg gcaccaccga agtgagcccg ggcctcgctg aaaagtggga
    13081 agtcagcgaa gacggtaaaa cctatacctt ccatctgcgt aaaggtgtga agtggcacga
    13141 caataaagaa ttcaaaccga cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga
    13201 tcgtcagaaa aacgcgcaaa acccgtacca taaagtttct ggcggcagct acgaatactt
    13261 cgaaggtatg ggcttgccgg agctgatcag cgaagtgaaa aaggtggacg ataacaccgt
    13321 tcagtttgtg ctgactcgac cggaagcgcc gttcctcgct gacctggcaa tggacttcgc
    13381 ctctattctg tcaaaagaat atgctgacgc gatgatgaaa gccggtacac cggaaaaact
    13441 ggacctcaac ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa aagattcccg
    13501 tatccgctac aaagcgtttg atggctactg gggcaccaaa ccgcagatcg atacgctggt
    13561 cttctctatt acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca
    13621 ggtgatgccg tacccgaacc cggcagacat cgctcgcatg aagcaggata aatccatcaa
    13681 cctgatggaa atgccggggc tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc
    13741 gctggatgac gtgaaagttc gccaggcact gacctacgcg gtgaacaaag acgcgatcat
    13801 caaagcggtt tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg
    13861 gggctataac gacgacgttc aggattacac ctacgatcct gaaaaagcga aagccttgct
    13921 gaaagaagcg ggtctggaaa aaggtttctc catcgacctg tgggcgatgc cggtacaacg
    13981 tccgtataac ccgaacgctc gccgcatggc ggagatgatt caggcagact gggcgaaagt
    14041 gggcgtgcag gccaaaatcg tcacctacga atggggtgag tacctcaagc gtgcgaaaga
    14101 tggcgaacat cagacggtaa tgatgggctg gactggcgat aacggggacc cggataactt
    14161 cttcgccacc ctgttcagct gcgccgcctc tgaacaaggt tctaactact caaaatggtg
    14221 ctacaaaccg tttgaagatc tgattcaacc ggcgcgtgct accgacgacc ataacaaacg
    14281 cgttgaactg tacaagcaag cgcaggtggt gatgcacgat caggctccgg cactgatcat
    14341 cgctcactcc accgtgtttg aaccggtacg caaagaagtc aaaggctatg tggttgatcc
    14401 attaggcaaa catcacttcg aaaacgtctc tatcgaataa ttaaaagcca tacaagactg
    14461 atggcaaagg caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa attctgcaat
    14521 gtattgaatt tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa
    14581 caaagcgcac tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc
    14641 catccggcag caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat
    14701 acagacacgc agttccaggc tgcgggtcac tatagagaat ccgggttatg ttgcagttta
    14761 ttctccgacg tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg
    14821 cctttgtcca catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct
    14881 cccctgagcg tcacgtgcag ctgctggctg aactcggctt agataaaccg atgtggcagc
    14941 agtatctcca ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc
    15001 gaattccagt atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct
    15061 gcgcgatgat ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac
    15121 gcggttccat tttcgatcac actgcagttg gcctggcgct gaccggctac tcgatgccga
    15181 tcttctggtg gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg
    15241 tctccggtcg cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta
    15301 tgctaatcga caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata
    15361 tgatcttgcc cgccattgtg ctgggtacta ttccgctggc ggtcattgtg cgtatgacac
    15421 gctcctcgat gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc
    15481 taacccgcat gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga
    15541 ccgttatcgg cctccaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct
    15601 tctcgtggcc cggtctgggg cgctggttga ttgacgcact gcaacgccgc gactatccgg
    15661 tagtgcaggg cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg
    15721 atctgctgta cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt
    15781 cacaggttac tgaaaataaa gtgattagcg caccggtgcc gatggccccg ttacaggagt
    15841 tctggcacta ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg
    15901 tgctgttcat cgcgatcttt gccaactgga ttgcgcctta taacccggcg gatcagttcc
    15961 gcgatgcgct gctcgccccg ccagcctggc aggaaggcgg cagtatggca cacttgctgg
    16021 gcaccgatga cgtcggtcgt gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc
    16081 tgctggttgg ctgtctggtg gtggtgttgt cgctgattat gggcgttatt ctcggcctga
    16141 tcgccggtta cttcggcggc ctggttgata acattatcat gcgcgtggtc gatatcatgc
    16201 tggcgctgcc aagtctgctg ttggcactgg tgctggtggc gattttcggc ccgtcgattg
    16261 gtaacgccgc gctggcgctg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg
    16321 ccgccgtgct ggtggaggtg aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg
    16381 gggcgatgcg ccagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc
    16441 aggcgtcgct cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca
    16501 tgggggcaca gccgccaacg cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg
    16561 cgcaaagcgc ctggtgggtc gtgaccttcc ctggtctggc gatcctgctg acggtgctgg
    16621 catttaacct gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag
    16681 aggttcgaga tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc
    16741 gcaccgttcc gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggtcggg
    16801 attgtgggtg agtccggctc cggtaagtcg gtcagttcac tggcgattat ggggctgatt
    16861 gattatccgg gccgcgtaat ggcagaaaaa ctggagttta acggccagga tttgcagcgt
    16921 atctcagaaa aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac
    16981 ccgatgacca gccttaaccc gtgctacacc gtgggtttcc agattatgga agcgattaag
    17041 gtgcatcagg gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag
    17101 gtcggtattc ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc
    17161 atgagccagc gcgtgatgat cgccatggcg attgcctgtc ggccaaaact gctgattgcc
    17221 gatgaaccga ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg
    17281 gagctacagc agaaagagaa catggcgctg gtgttaatta cccatgacct ggcgctggtg
    17341 gcggaagctg cgcataaaat cattgtgatg tatgcaggtc aggtagtaga aacgggcgat
    17401 gcacacgcca tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg
    17461 ccagaatttg ctcaggacaa agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag
    17521 tatgaccgcc cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt
    17581 cgcgctgaag aaccggcgct gaatatgctc gctgatgggc gtcagtccaa atgccattac
    17641 ccacttgatg atgccgggag gcccacacta tgagtacgca agaggccacc tcgcaacaac
    17701 cgctgttgca ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc
    17761 cggaacgtct ggtgaaggcg ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc
    17821 tggcagtagt gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga
    17881 tcgaaacgcc caccggcggc gagctgtatt atcaggggca ggatctgctc aagcacgatc
    17941 cgcaggcgca gaagctgcgt cggcagaaaa tccagatcgt cttccagaat ccctacggtt
    18001 cgctgaatcc gcgtaagaaa gttgggcaaa ttctcgaaga gccgctgctt attaacacca
    18061 gcttaagcaa agaacagcgt cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga
    18121 aaaccgagca ctatgaccgc tatccgcaca tgttttccgg cggacaacgt cagcgtatcg
    18181 ctattgcccg tggtctgatg ctcgacccgg atgtggtgat tgccgatgag ccggtttctg
    18241 cgctggacgt ctcagtgcgt gcgcaggtgc tgaatctgat gatggatttg cagcaggagt
    18301 tggggctttc ttatgtcttt atctcccacg acctgtcggt tgtggagcac attgctgatg
    18361 aagtgatggt gatgtaccta ggccgctgcg tggagaaagg aacgaaggac caaatcttca
    18421 ataacccgcg ccatccgtac acccaggcgc tgctctccgc gacgccacgc ctgaacccgg
    18481 acgatcgtcg tgaacgcatc aaactcaccg gtgaactgcc aagcccgctg aatcctccgc
    18541 cgggttgcgc cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc
    18601 cgcagctaaa agactacggc ggtcaactgg tagcttgttt tgctgttgat caggatgaaa
    18661 atccgcagcg ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca
    18721 acagtggcga caagcatagc aaaatgccgt aaagcagaat cagccaggct ttaaatccgc
    18781 gaagtttttc cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa
    18841 tgccatataa cgggcttccc aactgaaaga acaccagcac tgatacacga aacgaaaccc
    18901 aaatcgtcaa cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa
    18961 ttttcttggt atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt
    19021 ggaaaccgag ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag
    19081 aggtgatatg aatgatatgt ccagggatca cctgcgcggc cagtgccagt gccgagatat
    19141 tttgttcaaa ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa
    19201 aaaacaggat caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc
    19261 gggttgccag tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta
    19321 cctgaataaa taccgcagaa aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa
    19381 agacggaggc ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa
    19441 aaccgaacac gacaataatc cctactttga ccaccaccat tggcccagaa atcttaaaca
    19501 gtaatcgttc accaccagac gcaatcgcta tcagtacggc gaaaatagcg actttataaa
    19561 gtagagattg tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt
    19621 cgaaaaccac ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa
    19681 ccccgaggaa aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat
    19741 tacaggaatc gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag
    19801 gataagcaat gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg
    19861 gcatcagcac ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt
    19921 cgtagcgtgt aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt
    19981 cctgtccaga aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca
    20041 ggtggagaag tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc
    20101 gcgaggaggc gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga
    20161 tatgaactgc aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa
    20221 gattgccggg tgctgacagt aaaaagcaat cgtagaatca gcggcaagcc ggaaaggttc
    20281 cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa
    20341 aacccccgca cgttgcaggt atgcacctga caacatcacg ggggctaatc ttgaacccaa
    20401 acaactcaag aatagccgcg aaacgttggc attacaacac aggcggctat atgacgctcg
    20461 cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg
    20521 ccagtatgat cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct
    20581 gccgtaatgg caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc
    20641 gtgcttactg cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatctt
    20701 ggtattgaat aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac
    20761 tggtgagttt tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa
    20821 ccaccagatc ggagatagcc aggaagctgc tcggttggtc aatgacaatc ggtgccccct
    20881 gatgtggtgc cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc
    20941 tagggatatc acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt
    21001 ccggcaccac gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt
    21061 ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg
    21121 gataatggtt gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt
    21181 tatctttttc ggtaacgtcc agccagcggt taagcacggc ggtatcgtca taaaccggcg
    21241 aaccatcaaa gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca
    21301 tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca
    21361 tcaggtgctg ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg
    21421 ccggttgata cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg
    21481 ccgccgggcc actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg
    21541 accacagtgg atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga
    21601 tgttaatcac cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg
    21661 atttacgttt cgcctccgcg ttatagaaat tattcagcca ggcgttaagg ttcgccgttg
    21721 ttggcggtgc agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc gtcgccgcaa
    21781 cggttgccgc tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc
    21841 acaaggagaa acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca
    21901 caaaaacggt aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa
    21961 tggccccaat catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac
    22021 tgaaccccgc cacctgcgaa ccctggctca ttatgctttc cgggccaggc aaccaggtgt
    22081 catgccagaa caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc
    22141 tgtaacgcgg aatgggcatc agcagaaacg cggcaaacac caaattgagg agcgggtgga
    22201 agttaagata tcccgcccac aacaggccaa acttaaccag aaaatagaag ttccagccgg
    22261 aaaggccgcg ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag
    22321 tcattttttg gttgccctgg ctttttccgt gcggcgtaac gtcccggccg gtttaacata
    22381 acgaggttta gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc
    22441 cagatagccc agcgggaaaa atatcagtgc gcaaacgaca ataatttcaa tgatatcgct
    22501 gatggtcatc atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt
    22561 cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa
    22621 ggcagcggca ttccccattg ttctggggca agcaggcgca tctgcaccag ctcggcactg
    22681 atttgatcat cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg
    22741 aaaatatgat tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc
    22801 agccgattac cgccaatggt catgatatcg ccggtacggt tagggcgaca tagcgtctgg
    22861 gcttgctcaa cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg
    22921 gcaggcaaga gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc
    22981 tggaaaccac gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggt
    23041 acatagcgac taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt
    23101 ggcgcattcc acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg
    23161 gcgcgcaggc tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga
    23221 cgcagggtat gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag
    23281 accaccgtcg ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac
    23341 agttgccagt gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg
    23401 cgtttttcgt cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata
    23461 ccattttgtt gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag
    23521 aaggcaatat ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt
    23581 gaacggtatt cctcaagcaa caatgaaaat tgtttatcgt tattatttcc gggattaatt
    23641 accaaaagcg aacaatggtt taacctgctc catttattca ttttatccaa ccatgagcga
    23701 agccgctcgg caggaatgtt ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa
    23761 ttatggggat caatagaaca ctgcaaatca cgggccaaat agtatagacc tttttcatga
    23821 tttagcattg aaaataattt tattttttcc ggcccttgag aatcatctaa ttgaaagatt
    23881 ttcgccggat cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca
    23941 attgtttgat tcgccagact gatagcatct tcatggcgat cgacgttaaa ccaccagacg
    24001 ccgcctgctg gcatatgtcg cagctcatcc cataatgatg agataccgat agagaataca
    24061 gggtccacaa tgtccctcat gtcaccattt tgtctgatta tcagtttact agcgaaagct
    24121 aagaaataaa cctaccattg aaattaaaac ttatcagatt tagcatgtga atcaatttag
    24181 aaggtcacaa gacgtgtgat tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg
    24241 aaagtcaaaa agtgagcaaa ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca
    24301 cggttagaat tgtctcatga acggtacggt tatttcatag ggatcaagca aaatgaataa
    24361 caatgaacca gatactctgc ctgatcccgc gataggctat atcattccag aatgatattg
    24421 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
    24481 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
    24541 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
    24601 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
    24661 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
    24721 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
    24781 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
    24841 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
    24901 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa accaccagct
    24961 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
    25021 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
    25081 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
    25141 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
    25201 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
    25261 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
    25321 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
    25381 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
    25441 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
    25501 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
    25561 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
    25621 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
    25681 ttagccataa aacacagcat ctggatgaga aaaaagagct cggtcatttg agtgccgggg
    25741 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
    25801 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
    25861 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
    25921 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
    25981 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacacg tggattgtgc
    26041 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
    26101 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
    26161 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
    26221 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
    26281 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
    26341 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
    26401 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
    26461 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
    26521 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
    26581 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
    26641 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
    26701 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
    26761 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
    26821 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
    26881 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
    26941 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
    27001 tgccgcatat gatccatgcc agcctgacca actcgaagat ccatcgccac tctttctgga
    27061 gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg gtggcgctga
    27121 ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg gaagaagagt
    27181 acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctactcaac ctggtgggcg
    27241 ttgcggtagg catctggcgc tacttctatg gcccgccaac cgagatgctc accgtggtcg
    27301 tcagtatggt gtgggtgttc tacaacctga ttgttcttgg cggcgcagtt gcggtatcgg
    27361 tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc gcggcaattg
    27421 cccgcgaaga tggtcacctc ttctcgtgta ccgttcagga tttctccgac ggtggtttgg
    27481 ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat ctgttgctta
    27541 aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg ggtaatgaag
    27601 ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg cagtgtacgt
    27661 ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat aagccgctgg
    27721 aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg gcggagtttg
    27781 cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc tgggttgtat
    27841 cgtttattcc gcgccgcccg gagcggagcg aaacggcaca accatcggat caggctttgg
    27901 ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc agtggctatg
    27961 gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca accactgatc
    28021 aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg gcaagtgatg
    28081 tcaggcgagc agggagctga tgcccccatc gtggcgcaga acggtccttc gcgtgatgtg
    28141 aagctgacct ttgcgcaaat cgcgccgccg ccgggcagca tggtgctacg tggtattaac
    28201 ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc
    28261 aacctcgaat acacgccatc gccatcgtta ctgcctgtcc agtcgcagtt aaaggtttat
    28321 ctcaatgatg agctgatggg cgtgctgcca gtgaccaaag aacagttggg taaaaaaacg
    28381 ctggcgcaaa tgcccattaa tccactgttt attaccgact tcaaccgtgt gcggctggag
    28441 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gcaccacgct ttggctggat
    28501 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
    28561 tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac cttgccgatg
    28621 gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt cgcctcgtgg
    28681 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaattgccg
    28741 gatcgcaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct gcgcgatcat
    28801 ccggcggtaa aagccccggt gattgagatg attaaccatc cgcagaatcc ttacgtcaaa
    28861 ctgctggtgg tgtttggtcg tggcgacaaa gacctgttgc aggcagcgaa aggtatcgct
    28921 cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa accgctgcta
    28981 ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt cacatttggc
    29041 gaactgaaaa cctatgaaga acagttacaa tccagcggcc ttgagccagc agcgattaac
    29101 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
    29161 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
    29221 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgtctgctg
    29281 ctgcgtattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc tattccggcg
    29341 ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa cccgatgcca
    29401 ggcagttcgg tagataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
    29461 gacgattcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
    29521 gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca aaccatcacc
    29581 gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgagtac tgttggtttt
    29641 atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga tggcagcacc
    29701 attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa actgaaagac
    29761 gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc
    29821 cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga aacccagtca
    29881 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
    29941 cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat gcttaacgat
    30001 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
    30061 tccggtatca acagcctacg tgttggcgac gtttattacg taggtcatct gccgtggttc
    30121 gagcgcttgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
    30181 agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt
    30241 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat cgtgacgatg
    30301 ctgttgttgg ctgcctttag tgttcaggca gcctgtaccg ggcctgcctg ggagcagttt
    30361 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    30421 accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa cgaccgtgca
    30481 gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    30541 cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    30601 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    30661 tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag
    30721 gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt gggttttgct
    30781 gaggataaca gctggcgttt taaccccagc tacatgccgc cgacgctggc gcagtatttc
    30841 acccgctttg gcgcgccgtg gaccacgctg cgcgaaacca atcaacgttt attgctggaa
    30901 accgccccga aaggcttttc gccagactgg gtgcgctatg agaaagacaa aggctggcag
    30961 ctaaaagccg aaaaaacatt gatcagcagc tacgacgcta tccgcgttta catgtgggta
    31021 ggcatgatgc ctgacagcga tccgcaaaaa gcgcggatgc tcaaccggtt taaaccgatg
    31081 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    31141 gcgcagggta aaggaccggt cggtttttct gccgccatgc tgcctttttt acaaaaccgc
    31201 gatgcgcagg cagttcagcg ccagcgcgtg gccgataact ttcccggcag cgatacctat
    31261 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacatcgttt ccgcttctcg
    31321 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    31381 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    31441 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagat ctggtgcaac
    31501 agtcgttata tcggctggaa cttattgatc cgaataaccc ggacgtcgtt gccgcccgtt
    31561 tccgttcttt gttacgtcag ggcgatattg atggcgcgca aaaacagctc gatcggctgt
    31621 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctactttcca
    31681 cgccggatgg tcgtcaggca ctgcaacagg caagattgca ggcgactacc ggtcatgcgg
    31741 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    31801 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    31861 agttaaaacg catcaatgcg gatgcaccgg gcaatacggg cctgcaaaac aatctggcgc
    31921 tattgctgtt tagtagcgat cgccgtgacg aaggttttgc cgtcctggaa cagatggcga
    31981 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    32041 ccgtcagtga tgccagtgtg tcggcgctga aaaaatatct ctcgatcttt agtgatggcg
    32101 atagcgtggc ggctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    32161 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggtatggcg ggtaaagcca
    32221 ttcccgaact acaacaggcg gtgcgggcga acctgaaaga cagtgaagct ctgggggcgc
    32281 tgggccaggc gtattctcag aaaggcgatc gcgccaatgc agtggcgaat ctggaaaaag
    32341 ccctcgcact ggacccgcac agcagcaaca acgacaaatg gaacagtctg ctgaaagtaa
    32401 accgctactg gctggcgatc cagcagggtg atgctgcgct gaaagccaat aatcctgacc
    32461 gggcagaacg gttgttccag caggcacgta atgtcgataa caccgacagc tatgcggtgc
    32521 tggggctggg cgatgtggcg atggcgcgca aagattaccc cgccgctgaa cgctattatc
    32581 agcagaccct gcggatggac agcggcaaca ctaatgccgt gcgcgggctg gcgaatattt
    32641 accgccagca gtcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagccagc
    32701 ggcgcagcat tgatgatatc gaacgcagcc tgcaaaacgg ccgtctggca cagcaggcag
    32761 aggtactgga aaaccagggc aaatgggcgc aggcggcagc acttcagcgg caacgactgg
    32821 cgctggaccc cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg
    32881 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag ccgaacgacc
    32941 cggagcaggt ttacgcttac gggctgtacc tctcaggtca caaccaggac agagcggcgc
    33001 tggcgcatat caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta
    33061 atcgactgca aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag
    33121 aggctgaagc ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc
    33181 tggctgactg ggcgtaacaa cgacgtgatt acaccgccgc ccgcactgca tatcagaatg
    33241 tcctgacgcg ggagccaact aacgccgatg ccattcttgg tctgacggaa gtggatattg
    33301 ctgccggtga caaagcggcg gcacgtagcc agctggcgaa actgcctgcc accgataaca
    33361 cctcgctgaa cactcagcgg cgcgtggcgc tggcacaggc gcagcttgga gataccgcag
    33421 cggcgcagca gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    33481 aaagcgcgat ggtgctgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc
    33541 aggcgctgga aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc
    33601 aggataacga cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac
    33661 gcggcgtgcg cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg
    33721 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta
    33781 ccatgttgca ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg
    33841 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    33901 gcacctgtac attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg
    33961 tggcggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    34021 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    34081 acaccgttaa cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa
    34141 aagactcccc gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc
    34201 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc
    34261 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    34321 ataaggtcat taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    34381 attacgacaa agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    34441 aatacctgtc gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    34501 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    34561 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    34621 gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    34681 actggtttgt tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt
    34741 ccctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc
    34801 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    34861 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    34921 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    34981 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    35041 cgcaactgga aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag
    35101 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    35161 gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc
    35221 gtccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg
    35281 tctactcgct cgaacgtccg gcaaatccgc agccaatagc ctatctggtg ctacaggcgg
    35341 attccttccg tatgtataag ttcgtgatga gcactctctc aacgttagtg accatttact
    35401 tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc
    35461 atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc
    35521 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgctg gtgcgcagtt
    35581 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    35641 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg
    35701 ttgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    35761 cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    35821 aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca
    35881 ttgccaacgg tgtacaggaa ccgtggcacg caatcacttt gggtcaacaa gtactcacta
    35941 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    36001 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    36061 catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg
    36121 aagcagccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    36181 agtttgcaat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    36241 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    36301 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    36361 gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    36421 ctgcgctgca actgatgcac ccgaatatgg tggcggatat gctggaactg ttaacccgct
    36481 atcgcattca gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc
    36541 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    36601 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    36661 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga
    36721 ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg
    36781 agactgaagc acaacgagac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    36841 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc
    36901 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    36961 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    37021 ttttaaggct gcaggtaccc ccataacctt acaagacctg tggttttact aaaggacacc
    37081 ctatgaaaac ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg
    37141 gtattctcct tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg
    37201 gcttcgttaa gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg
    37261 gcattgcggg catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt
    37321 actttgaaat tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc
    37381 agcctggtgc cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagaggttt
    37441 acgccgatca ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga
    37501 gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt
    37561 ttggttttgc gctccaccgt ctgggcagca aaggccaact gatttttaac gtgatcgaaa
    37621 gtttctcgca ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg
    37681 cgttcggggc aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg
    37741 ggcagctgat tatctgtttc tacattacct gtatcctgtt tgtggtgctg gtattgggtt
    37801 caatcgctaa agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac
    37861 tgctgattgt actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga
    37921 tggagaaact cggctgccgt aaatcggtgg tggggctggt catcccgaca ggctactcgt
    37981 ttaaccttga tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca
    38041 ctaacagtca gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt
    38101 ctaaaggggc ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg
    38161 cggtgggcca tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt
    38221 cagaagctcg tgcgctaact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta
    38281 agtgggtgaa agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg
    38341 atggcaaaac gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc
    38401 gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc
    38461 aggtttattt cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt
    38521 ttaacgtcat ttagactgac cgacaacggg cagttttttt attaccagga tagttgatca
    38581 ggggttcaca tgcagggcat aaaaattcga cttttagcgg gcggtttgct gatgatggcc
    38641 actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca acaggggacg
    38701 ctttccaacg gtttacagtg gcaagtgctg accacccccc agcgtcccag cgatcgtgtt
    38761 gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca gagcggttac
    38821 agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc agcacaggcg
    38881 cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt aattgtctct
    38941 tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgattt gctgaaagaa
    39001 gcgctctctt atctggcaaa tgccacaggc aaattgacca tcacaccaga aaccatcaac
    39061 cacgcgctgc aaagtcagga catggtggca acctggcctg ccgatactaa agagggctgg
    39121 tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg atcctgccga tccgctgaaa
    39181 caacccgttg aagcggaaaa aattaaagat ttctatcaga aatggtacac cccggatgca
    39241 atgacgctac tggtggtggg aaacgtggat gcgcgctcgg ttgtcgacca aatcaacaaa
    39301 acgtttggcg aactgaaagg caaacgtgaa acgccagctc cggtgccgac gctttctccg
    39361 ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc gtcaggaccg gttatctatc
    39421 atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg ctactggcgt
    39481 gcggacctgg cccgcgaggc gctgttctgg catgttcagc aagcgttaag cgccagtgac
    39541 agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt atctgcgtgc gcagtgtgcc
    39601 atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt ggcgcgtgaa
    39661 ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt agtggcgcaa
    39721 aagaaactgg agctgcagaa actgtttgcc gcctatgcac gggctgatac cgatattctg
    39781 atgggccagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc ggagcagtat
    39841 cagaaactgc ggcaggattt ccttaacagc ctgactgtgg agatgttaaa tcaggatctg
    39901 cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg cgagccggaa
    39961 tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc taccgccgct
    40021 gcgaccacct ctgtcgccac ggatgacgta catcctgaag tgacggatat tccacctgca
    40081 cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga tgagctggat
    40141 agaggggtaa cactttatcc agccttttgc atatgctgcg tttacgctgg catcgcctca
    40201 cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg cccgcgtttc
    40261 gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca gataaccggc gctgaaagag
    40321 tcgccagctg cggtggtatc gatcactttt tctttcggca gtttcaccgc cggaacatca
    40381 actaacgctt caccagcaat ggacaccagg caagaatccg ccccgcgttt caccaccact
    40441 tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg ttgaccccac
    40501 agcgcgtctt cgtcgtccag cgtcaggaag gcgatatccg tgcattccag catttgctgg
    40561 tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt attgtcgaaa
    40621 atcacttttc cgcctttggc gcggcattcg tgcagcaggg aaagcagctt ttcgcggctg
    40681 gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa attcgccagc
    40741 tcttcgcaaa tcgccgcaga ctgctcgctc gccagccaga atttggcggc ggcttcgtta
    40801 cgccagtagt agaacgtacg ttcgccggtg ctgtcggttt caatgtagta aaggcccgga
    40861 agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgtg ccaggcgtcc
    40921 agcatctgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac ggttaatgcc
    40981 gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc gccgaaaccg
    41041 cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat cacggcaatc
    41101 tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg cgctgtggtg
    41161 gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca gaacgtattc
    41221 ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc caggcggtaa agttctgccc
    41281 ttacgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac aggacatctt
    41341 tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc gagagcttgc
    41401 aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg atctatcaaa
    41461 catgcgggcg gttaatggcc gtggagctat taacggtggt cacgcatccc ttgaaccctt
    41521 cgcaactcct gccgccggat cgctatttta ctgaaatcac cgtcagccat cggatggagg
    41581 ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttgta gagcacggcc
    41641 tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag caaccaaaaa
    41701 tcctgcgcca gattgagcgt cttccctggc tgcgtttcga actggtggag catatccgtc
    41761 tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg ctggatgatt
    41821 ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac tacatcaaaa
    41881 tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga aggccgcaca ctcttttctc
    41941 agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc gtagaaacac
    42001 cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc tggtttcttt
    42061 cacgcccggc accgatggaa acgctgaata cggcggttct ggcgctataa gctgcctcat
    42121 tttccgccgg gctggactat ctttaggact ggcacaggaa gaatatgagg aagcgaacga
    42181 tgagcaaggc aggcaaaata accgctgcga tttcaggggc tttcttgttg ttgattgtcg
    42241 tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc atcaaccaga
    42301 aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg cgatctgggc gtggtgtggg
    42361 agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggcctcat gtacatgcag
    42421 aagacatcat tcttggtaat ccaccggata ttcccgaagt cacgatggtg catttgccgc
    42481 gagtagaggc aacgctgaca ccgctggcgc tgctgaccaa aacggtctgg ctgccgtgga
    42541 tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc tgaaaagaac aataactgga
    42601 cgtttaatct tgccaacgat gacaacaaag acgcgaatgc aaagccgtcg gcatggtcgt
    42661 ttcggctgga taatattctt ttcgatcaag ggcggatcgc cattgatgac aaagtaagca
    42721 aagcggatct ggaaattttt gttgatccgt taggcaagcc gctgccattc agcgaagtta
    42781 ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacattttt ggcctgaagg
    42841 cgcagggacg ttataacggt gaaccgctca cgggtacggg aaaaataggc ggtatgctgg
    42901 cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga tttccgttca ggtaataccc
    42961 gtgttgcttt tgatggcgtc gtgaatgacc caatgaagat gggcggtgtc gatttacggc
    43021 ttaaattttc tggcgattca ctgggtgatc tctatgaact gacgggcgtt ctgctgcccg
    43081 ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact gaaaaatcgt
    43141 cggtctttga ttatcgcggt tttaatgggc gaattggcga tagcgatatc cacggttctc
    43201 tggtctacac caccggaaag caacgaccaa aactggaagg tgatgtcgag tcgcggcaat
    43261 tgcggctggc ggacctggga ccgttgattg gcgttgattc cgggaaaggg gcagaaaagt
    43321 cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc aaagtgctgc
    43381 cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt cgcttcaaag
    43441 ggcggcgcat tgagcatggc agtagcctgc cgattagcga tctttctact catatcatcc
    43501 tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catggcgggc ggcagcattg
    43561 cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca gatattcagg
    43621 ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga actgatgcag aagacgctgg
    43681 gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa ctcggtggcg gcgcttttag
    43741 gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc cgcaacctga
    43801 tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt ggtgacgatg
    43861 aggtgcgggt gaactgcgcg gcggcgaatc tgaatattgc caacggcgtg gcgcgcccgc
    43921 agatttttgc tttcgatact gagaacgcgt tgattaatgt tatcggcacg gcaagttttg
    43981 cttcggaaca gctggatttg actattgatc cggagagtaa agggattcgg attatcacac
    44041 tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg gtgaaagccg
    44101 gaccgctgat tgcccgtggt gctgttgctg cggcactggc aacgctggta acaccggcgg
    44161 cggcgttact ggcactgatc tcaccttccg aaggggaggc taatcagtgt cggacgatat
    44221 tgtcgcagat gaagaagtga tgataattgc cggatgtctg acatccggca attaaacaga
    44281 tttagatttt tggcaaagtg tgctttgttc atgccggatg cggcgtgaac gccttatccg
    44341 gcctacaaat ccttgtgaat tcaatatatt gtatggaaat tcagtcctga taagcgtagc
    44401 gcatcaggct tttcacactt attacaacga ctgatgtcgc gtctcatggg tcagcagcag
    44461 ggcgattaac gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta
    44521 gttagcctgc aaccaggttg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac
    44581 gttgtaagag aacgatgctc cggtgtaacg cacttctgtc ggaaacagct ctggtaacag
    44641 cgcacccatc ggcccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa
    44701 aaccagaatc gggttgccag aaccgagcag cgggttaaag gcgaacagcg cgaacaggat
    44761 gatcagcgtg gtgatgatca ccatgctttt acggcgacca aaggcatcag ccagtaatcc
    44821 ggcgactggc accatcacgc caaaaccaat aactgccatc atcagcatcc acaacacttc
    44881 gttacgcggc aggccaagcc caactggcgc ggcggcggta ctgaaggtca tagagtagac
    44941 cgtcatgatg taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac
    45001 gcgaacatgt ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc
    45061 gactttctca aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac
    45121 cagcaccgcc gagaagataa atggcacacg ccagccccag ctcataaact gctcatcggt
    45181 cagtagccag gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc
    45241 tagctgtggg aaggagccat acagtgcacg tttgcgcggt ggggcgtttt cagtcgccag
    45301 cagcgccgcg ccgccccatt caccgcctaa gcccagaccc tgaccaaatc gagccagcgc
    45361 cagcagcagt ggggcgaaaa taccaatcgt ggcatagccc ggcagcagac caatcaccac
    45421 ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc
    45481 gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg acgaaggcga tggcgaaggt
    45541 ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc gggaagaaga tatgcggaaa
    45601 cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ccgtgccaat
    45661 gagagaggcg acaaggactt tattacgcga gttgatcggc gtgtattctt gttcgtggtc
    45721 gagtgttgtg gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata
    45781 ttacgcacag caaaagtgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca
    45841 ggcaaaaagg ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat
    45901 tttataaata gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata
    45961 tgctggggtt atgagttgga gtgaaattgt ttgctcccaa atagatggct gaagaggctc
    46021 tggggaagag tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct
    46081 tatccggcct acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc
    46141 gcatccggcc tattttaagg ctgcgttttc cctggcattc gcgggtcgtc tttatattcg
    46201 gcggtggcaa tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc
    46261 atcagcccca gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg
    46321 gtgtaggtca tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga
    46381 atcagggctt ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc
    46441 agatagttgg cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac
    46501 tcaatgctgt tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag
    46561 gtgatcgaaa gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc
    46621 agatatttta cccagaactt ctcctgatct tgcggcgagc gttcccagac atcgcgcgac
    46681 tgggcacgaa tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc
    46741 gccaggccga caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc
    46801 aacgtggcgg ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt
    46861 agcaacatcg gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc
    46921 gggatcatcg acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga
    46981 tcgttaaagc gttctgtcgc gcgaataagg tgcgcaataa ccggttggcg ttggactttt
    47041 tcggcagtgg tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta
    47101 tccaactgct taatcggctc gtgctccaga tcctgggtgg gacgtttgat ctcgttttcc
    47161 tgcgtcatcc gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag
    47221 tccaggtgct ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa
    47281 agctcccgac gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagcctggc
    47341 aacacttcaa taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga
    47401 ataatcccca gaccggcaag accggcggcg agatagtttt cgctactgtt taccgtcaac
    47461 atgccgccag acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga
    47521 tgtacaccca ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt
    47581 tgcggataac caaagcgcgt cagatagtgc ggactggcac agttgaccat ggttagtttg
    47641 ccgaggggac gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca
    47701 aaaccatcat gaagaatatc taccggacgg ttatggctac tcagttccag ctcaattccc
    47761 ggatgctgat agagaaattc cgacagacgc ggcagtaaca ggtttttcgc gattcctgac
    47821 gggatgtcga tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc
    47881 agttcgctga gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc
    47941 gtgagtttga cccgtcgcgt agtgcgctgg agaagctggg tgccaagttg atgttccagt
    48001 gcctgtatct ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc cgcgcgggaa
    48061 aaactttcca gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct
    48121 ttgaccgatt gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt
    48181 tctaacatct aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca
    48241 ggaaaagggc ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc
    48301 agtgattccc tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca
    48361 ttgccatttg ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta
    48421 accaccaata taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc
    48481 aggcgtttag ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa
    48541 tggccaccgc caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt
    48601 aagtcttttt aacgtattca ggtggagatt cagttatgca aatagtcatg tttgacaggc
    48661 agtcaatatt tattcatgga atgaaaatca gtttacagca gcgtattcca ggagtgagta
    48721 ttcagggggt cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct
    48781 tagttatgct cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg
    48841 tggtgcagtt tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt
    48901 tacaggaagt tatccatttt aatgtgctgg ccattgtgcc tcgcgattca accgtcgaga
    48961 catttgcgtt ggtggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc
    49021 ggacgacgcc ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga
    49081 ttttaaccat gttagcggcg ggcgaatcaa ataaggagat cggcagagcg ctgaatatca
    49141 gtaccggaac ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc
    49201 gcacccaggc ggcaatgatg ttaaatatct cctcctga
//
LOCUS       DADKXM010000016        47042 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.016, whole genome shotgun sequence.
ACCESSION   DADKXM010000016 DADKXM010000000
VERSION     DADKXM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.016"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JQR05_000918"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JQR05_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY5323136.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLCFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JQR05_000919"
     CDS             complement(1614..1958)
                     /locus_tag="JQR05_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY5323137.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQR05_000920"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQR05_000920"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY5323138.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQR05_000921"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQR05_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY5323139.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLVWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JQR05_000922"
     CDS             complement(6144..6842)
                     /locus_tag="JQR05_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY5323140.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQHP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQR05_000923"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQR05_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY5323141.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JQR05_000924"
     CDS             7537..8574
                     /locus_tag="JQR05_000924"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY5323142.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMVANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JQR05_000925"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JQR05_000925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY5323143.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JQR05_000926"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JQR05_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY5323144.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIAAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JQR05_000927"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JQR05_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY5323145.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JQR05_000928"
     CDS             10745..11626
                     /locus_tag="JQR05_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5323146.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JQR05_000929"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JQR05_000929"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY5323147.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JQR05_000930"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JQR05_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY5323148.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JQR05_000931"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JQR05_000931"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY5323149.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JQR05_000932"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JQR05_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY5323150.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JQR05_000933"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JQR05_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY5323151.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JQR05_000934"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JQR05_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY5323152.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JQR05_000935"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JQR05_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY5323153.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JQR05_000936"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JQR05_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY5323154.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JQR05_000937"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JQR05_000937"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY5323155.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPSPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JQR05_000938"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JQR05_000938"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY5323156.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JQR05_000939"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JQR05_000939"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY5323157.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JQR05_000940"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JQR05_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY5323158.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JQR05_000941"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JQR05_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY5323159.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JQR05_000942"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JQR05_000942"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY5323160.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVDAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JQR05_000943"
     CDS             24893..25273
                     /locus_tag="JQR05_000943"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY5323161.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JQR05_000944"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JQR05_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY5323162.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFS"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JQR05_000945"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JQR05_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY5323163.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26283..27131)
                     /locus_tag="JQR05_000946"
     CDS             complement(26283..27131)
                     /locus_tag="JQR05_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY5323164.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27340..27975
                     /gene="yfhb"
                     /locus_tag="JQR05_000947"
     CDS             27340..27975
                     /gene="yfhb"
                     /locus_tag="JQR05_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY5323165.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28000..28536
                     /gene="tadA"
                     /locus_tag="JQR05_000948"
     CDS             28000..28536
                     /gene="tadA"
                     /locus_tag="JQR05_000948"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY5323166.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28533..30089)
                     /gene="mltF"
                     /locus_tag="JQR05_000949"
     CDS             complement(28533..30089)
                     /gene="mltF"
                     /locus_tag="JQR05_000949"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY5323167.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSILWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30347..34234
                     /gene="purL"
                     /locus_tag="JQR05_000950"
                     /gene_synonym="purI"
     CDS             30347..34234
                     /gene="purL"
                     /locus_tag="JQR05_000950"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY5323168.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34810..36237
                     /gene="qseE"
                     /locus_tag="JQR05_000951"
     CDS             34810..36237
                     /gene="qseE"
                     /locus_tag="JQR05_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY5323169.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSISFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36402..37115
                     /gene="qseG"
                     /locus_tag="JQR05_000952"
     CDS             36402..37115
                     /gene="qseG"
                     /locus_tag="JQR05_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY5323170.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37105..38439
                     /gene="glrR"
                     /locus_tag="JQR05_000953"
     CDS             37105..38439
                     /gene="glrR"
                     /locus_tag="JQR05_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY5323171.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDQSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38500..38838
                     /gene="glnB"
                     /locus_tag="JQR05_000954"
     CDS             38500..38838
                     /gene="glnB"
                     /locus_tag="JQR05_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY5323172.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38883..40073)
                     /gene="hmpA"
                     /locus_tag="JQR05_000955"
     CDS             complement(38883..40073)
                     /gene="hmpA"
                     /locus_tag="JQR05_000955"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY5323173.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40400..41653
                     /locus_tag="JQR05_000956"
     CDS             40400..41653
                     /locus_tag="JQR05_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY5323174.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41851..43044)
                     /locus_tag="JQR05_000957"
     CDS             complement(41851..43044)
                     /locus_tag="JQR05_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY5323175.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYHGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43342..46437
                     /locus_tag="JQR05_000958"
     CDS             43342..46437
                     /locus_tag="JQR05_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY5323176.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVANH
                     HIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKRFE
                     QYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNALLD
                     DAVALTPATAIWGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPLAA
                     QDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRADFP
                     QAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSKAG
                     GYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQREK
                     LLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAAEM
                     LNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEECW
                     FARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAARYL
                     DNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLWHL
                     TGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHAAL
                     HYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYYND
                     QPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSLYG
                     DLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFNYV
                     H"
     gene            46534..>47042
                     /locus_tag="JQR05_000959"
     CDS             46534..>47042
                     /locus_tag="JQR05_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY5323177.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINC"
BASE COUNT        11348 a        11857 c        12499 g        11338 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctctgttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacaaga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtctgcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggctag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccagacca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 tgttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtca atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggta
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa attatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgctgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattt gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caagcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaaccgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgctt cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgt cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgccgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttgacgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctca taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tctcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattgat gcatttttta gtgattaaca
    26221 gcgccataag gaaactctca aaacaacaca aaccgggcgg gcattatacg cgcattcttt
    26281 actcagacca gttttttcac cagcgcttca ctatgacgaa tacgttctgg tgccagttcc
    26341 agatcctgct gaatcagcgc aatgaacagc aaatccgtta acattccctg agcgtgacaa
    26401 gcagagattg aagcactgtt tgtcgcttgt tcttcggcaa tggtatacag gcaatgagaa
    26461 gcacgctgtt gcagggcatt cggagtaaag ccggtaatcg ccagcacttt tccgcccact
    26521 cgcagcatct catctgccgc caggtttaac tcgcgtcgta caccggtgta ggaaatggct
    26581 aataacagat cgtcagggga cgacgcctgt actgttgcga gtagcgcatg catatcgcgc
    26641 agtgcggcag cattgaagcc aatcttcatc agcttccagg caaagttttg cgccaccaga
    26701 cccgaagcgc caataccggt cagaattatc cgccgcgcag agcgcaacat tgttacgcat
    26761 tcatgcagtt tctcttcact attaacgttt agcgttgcgt acatagcggc agtattttct
    26821 ttaatcagtt tttcgccgac cagccgtaac ggatcatcac cgcggatttg gttatgaatg
    26881 ggcacggagg gcgattccgg ctggcttgcc agcgcttcac tcaacgccaa cttaagcgcc
    26941 ggaaaacctt tatagccgag tttttgcgcg aacttcacga cgctggactg actgactccg
    27001 gcttcgttgg ccagttgctg agagcttaaa tggcgcgccg tatcaggttg tagcagcaga
    27061 taatccgcca gttttttatc actctgggca agcccctggt aacgctgacg gatacgaagt
    27121 aagccgttca tatctttcct cgcgatgaaa tagtggcgac aacaggcaat atgagacaca
    27181 cgcgagtgaa ttttatattc cattgccgct aattgccatg aattaaaaat tccagttagc
    27241 catgatgcgg tcgattgttg ccggaaaagt gatgatctat ctgttaggct atgtagtagt
    27301 gaaaattaat caggatgttt cagtccagag gagtatggtt tggcaactca cgagcgtcgt
    27361 gtggtgtttt ttgacttaga tggaacattg catcagcagg atatgttcgg cagttttctg
    27421 cgctatttac tgcgtcgcca accgctgaat gcgttacttg tcctaccgtt gttaccgatt
    27481 atagccattg cgttattgat aaaaggtcgt gcggcacgct ggccgatgag tctgcttctg
    27541 tgggggtgca cttttggtca cagcgaagca cgtttacaga cgttgcaggc cgatttcgtg
    27601 cgctggtttc gcgacaatgt taccgccttt ccgctggttc aggagcgatt aaccacctac
    27661 ctgttaagtt ccgatgctga tatctggttg attaccggct ctccgcagcc gctggttgaa
    27721 gcggtttatt tcgatacgcc ctggctgccg cgggttaatc ttatcgccag ccaaattcag
    27781 cgtggctatg gtggttgggt attgacgatg cgttgtctgg gacatgaaaa ggtcgcacaa
    27841 ctggagcgca aaatcggcac gccgctgcgg ctgtacagtg gctatagcga cagtaatcag
    27901 gacaatccgc tgctttattt ctgtcagcat cgttggcgag taaccccgcg cggtgaactc
    27961 cagcaactgg aatagagtaa agcatagcgt ccgtgtataa tgcgccgcgc ttttataacc
    28021 ggagttttct ttttgtctga agtcgaattt agccacgaat actggatgcg tcacgcgctg
    28081 acgctggcga aacgtgcctg ggatgagcgg gaagtgccgg tcggcgcggt attagtgcat
    28141 aacaatcggg taatcggcga aggctggaac cgcccgattg gtcgccatga tcccaccgca
    28201 catgcagaaa tcatggccct gcggcagggt ggtctggtga tgcaaaatta tcgtctgatc
    28261 gacgccacgt tgtatgtcac gcttgaacca tgtgtaatgt gtgccggagc gatgatccac
    28321 agtcgcattg gtcgcgtggt ctttggtgcg cgtgacgcga aaactggcgc tgcgggatct
    28381 ttaatggatg tgctgcatca tccgggtatg aatcaccgag tggaaattac ggaaggaata
    28441 ctggcggatg agtgcgcggc gttgctcagt gacttctttc gcatgcgccg ccaggaaatt
    28501 aaagcgcaga aaaaagcgca atcctcgacg gattaatttt gtttctcttc actccctttc
    28561 ctggaaaaca gcagagaggg agaatgggtc aaatagtttg atgacgactg ggaaagaaac
    28621 gagagaaaag gaaatttctc tttgcccaac tccgtaggcg ataccgccgg ataatcctgc
    28681 gccagttgca tcgccgcttc tgtagcctgc ttctctttct cttgcagata acccaccagg
    28741 ctaatctgat acttacgaat attttcgaca taagcgtagg cttcatgccc acgagcgtag
    28801 ccgtaagtca gcttgctgta atagggtttc tggctaagta aaggcagacg ctgttttacg
    28861 tcagcccaac tgtcaggatt ccctttggtt tttgccgtca gggcgcgggc atccagcata
    28921 tgcgcatagc ccatattgta cgcagcgagg gcaaaccaga tccgttcgtt ctccggcaca
    28981 ctttccggca ctttactcat catatcctgc aaataacgca cgccaccgct gatgctctgt
    29041 tcggcatcgg tacgatccgt aatgccgagg ctttgcgcgg tatttttggt taacatcatc
    29101 atgccgcgca cacccgtcgg tgaagtggcc tgtgcatccc agtgcgattc ctgataagca
    29161 atagcggcca gcaaacgcca gtcaatttct tcggcgtatt tctcaaacag gggctttaac
    29221 tgcggcagta ccgcatcgac ggcgcgtaaa aatgtgcgcg tatcgacgta atcaaaatca
    29281 tcgccatgcc ccaggtattt ctcttcaatg cgtgccagcg taccgtcttc attcatttcg
    29341 ttgaagaagt cgagcagggc ggcggaaagg gtattatcgc catctaacgg gctaaaccaa
    29401 gtcaccggtt gttcatcggt gatatcgagc gctacggcga gctccgggtg aacgcgctga
    29461 aacaggctga tggcgacaga atcagcaatg gtgtaatcga gttttccttc gatgacatct
    29521 tccattaatt ccgcagagcc ttttttgtcg tctaccttcc agcttaattc cgggaatttt
    29581 gtttctttca gggtctggag atcgttaacc accacatgac ccggtgcaac ggtaagttgc
    29641 tccgccgtca ggttgcccag cgtacgtggg cgatactgac ccactttata aaccagttgt
    29701 tgtgacacgg aataataggt agggccaggc tgataatttt ttacccgctc actgttatag
    29761 accagtcctg ccgccagcag gtcggcgtta ccattatcaa ggtcgtcaaa cagctggctg
    29821 atattctgcc gcacggtcac tttcagtttt acgccgaggt aatcggcaaa ctgtttcgcc
    29881 agttcgtaat ccaggccaaa aggtttcccg ttgatttcgt tataagtcag gggagtatga
    29941 atggtgctca cacgcaactc tccccgcgct tgaatggcgg cgatacggtt gtcggcttta
    30001 ccaaaccaga gaatggatgg ccagagagcg accgcgagca gcagtgccag aatgccgatg
    30061 aacagataat taatctttaa ttttttcaat tagttaattc tctgtgtcgt gcgcgtccca
    30121 gcttgaaaaa acgtaataat agtgaaaggt ttactcataa atgagcggca ttttgcgtaa
    30181 acctgcgcca gatggcaact tattacagcc attggcggca cgcgttgcta attcacgatg
    30241 gtgattttat ttccacgcaa acggtttcgt cagcgcatca gattctttat aatgacgccc
    30301 gtttcccccc cttgggtaca ccgaaagctt agaagacgag agacttatga tggaaattct
    30361 gcgtggttcg cctgcactgt cggcattccg aatcaacaaa ctgctggcac gttttcaggc
    30421 tgccaggctc ccggttcaca atatttacgc cgagtatgtc cattttgctg acctcaatgc
    30481 gccgttaaac gatgatgagc acgcacaact tgaacgcctg ctgaaatatg gtccggcact
    30541 cgccagccac gccccgcaag gcaaactcct gctggtgacc ccgcgtcctg gcaccatctc
    30601 tccctggtct tcgaaagcga ccgatattgc ccataactgc gggctacaac aggtaaaccg
    30661 ccttgagcgc ggcgttgctt actatataga agccggtacg ctgaccaatg aacaatggca
    30721 gcaggttacc gctgaactgc acgaccgcat gatggaaacg gtcttttttg atttagatga
    30781 tgcagagcag ttgtttgccc accatcaacc gactccggtc acaagcgttg atttgctggg
    30841 gcagggccgt caggcgctga tcgacgctaa cctgcgtctt ggcctggctc tggcggaaga
    30901 tgaaattgac tatctgcagg atgctttcac aaagcttggt cgtaacccga acgacatcga
    30961 gctgtatatg ttcgctcagg cgaactctga gcactgccgc cacaaaattt ttaacgccga
    31021 ctggattatc gacggtgaac aacagccgaa atcgctgttc aggatgatca aaaatacttt
    31081 cgaaaccacg ccagatcacg ttctctctgc ttataaagat aacgccgccg taatggaagg
    31141 ttctgaagtg ggccgctact ttgctgacca cgaaacgggc cgctacaatt tccatcagga
    31201 accggcgcac atcctgatga aagtcgaaac tcacaaccac ccgacggcga tttctccgtg
    31261 gccgggcgcg gcgaccggtt ccggcggtga aatccgcgat gaaggtgcca ccgggcgcgg
    31321 cgcgaagccg aaagctggtt tggttggttt ctccgtatcc aacctgcgaa ttcctggctt
    31381 cgaacagccg tgggaagaag atttcggtaa gcctgagcgc attgttaccg cgctggacat
    31441 catgaccgaa ggcccgctgg gcggtgcggc gtttaacaac gaatttggtc gtccggcact
    31501 gaacggctac ttccgtactt atgaagaaaa agtgaacagc cacaatggcg aagagctgcg
    31561 cggttatcac aaaccgatca tgctggcggg cgggatcggc aacattcgcg ccgatcacgt
    31621 acaaaaaggc gagatcaacg tcggtgcgaa gctggtcgtt ctcggcggcc cggcaatgaa
    31681 catcggtctt ggcggcggcg cggcgtcttc catggcgtct ggtcagtctg atgccgacct
    31741 cgactttgct tccgtacagc gcgacaaccc ggagatggag cgtcgttgcc aggaagtgat
    31801 cgaccgttgc tggcagcttg gtgatgctaa cccaattctg tttatccacg acgtcggtgc
    31861 gggcggtctt tctaacgcga tgccggagct ggtgagcgac ggcggacgcg gcggtaaatt
    31921 tgaactgcgc gatattctaa gcgacgaacc gggcatgagc ccgctggaaa tctggtgtaa
    31981 cgaatcccag gaacgctacg tgctggcggt tgctgccgat caattaccgc tgtttgacga
    32041 actgtgtaag cgtgagcgcg caccctacgc ggtgattggt gaagcgaccg aagaactgca
    32101 tctttctctg cacgatcgtc attttgataa tcagccgatc gatctgccgc tggacgtcct
    32161 gcttggtaaa acgccgaaga tgacccgcga tgtacaaacg ctgaaagcga agggcgatgc
    32221 gctggtccgt gaagggatca ccattgcaga cgcggtgaaa cgcgtactgc atctgccgac
    32281 cgtggcggag aaaactttct tggtgaccat cggtgaccgc agcgtaaccg gcatggtggc
    32341 gcgcgatcag atggtggggc cgtggcaggt gccggtcgct aactgcgcgg tcactaccgc
    32401 cagcctcgac agctactacg gtgaagcgat ggcgattggc gagcgtgcgc cggttgcgct
    32461 gctggatttc gccgcctctg cccgtctggc ggtcggtgaa gcgttaacca atattgccgc
    32521 aacacagatt ggcgatatca aacgcattaa actttccgcc aactggatgg cggcggcagg
    32581 ccaccctggt gaagatgcgg gcctgtatga agccgttaaa gccgtgggtg aagaactttg
    32641 tccggcgctg ggcctgacga tcccggtggg taaagactct atgtcgatga aaacccgctg
    32701 gcaggaaggt aacgaagagc gcgaaatgac gtcgccgctg tcgctggtga tttctgcatt
    32761 tgcccgcgtg gaagatgtac gtcacaccat cacgccgcag ctttgtaccg aagataacgc
    32821 actgctgctg attgatttgg gcaaaggcaa taacgcgctg ggcgcaacgg cgctggcgca
    32881 ggtttatcgt cagcttggcg acaaaccggc agatgtacgc gatgttgccc aactgaaagg
    32941 cttctatgac gcgattcagg cgctggttgc acagcgtaag ctgctggcgt atcacgaccg
    33001 ctctgatggc ggcctgctgg taacgctggc ggaaatggcc tttgctggtc attgtggcat
    33061 tgacgcggat atcgcctctc tgggtgacga tcgcctggcg gcgttgttta acgaagaact
    33121 gggtgcggtg attcaggttc gtgccgctga ccgtgaagcg gtcgagtccg tactggcaca
    33181 gcatgggctt gcggattgcg tccattatgt tgggcaggcg gtttccggtg accgttttgt
    33241 gattaccgcc aacgggcaga ctgtattcag cgaaagccgc actacgttgc gtgtctggtg
    33301 ggcagaaact acctggcaga tgcagcgcct gcgtgacaac ccggagtgtg ccgatcagga
    33361 gcatcaggcg aaatctaacg acgccgatcc gggcctgaat gtaaaactgt cgttcgatat
    33421 caacgaagat gtggcagcac cgtatattgc cactggcgca cgtccgaaag ttgccgtact
    33481 gcgtgagcag ggcgtgaact cgcatgttga aatggcggca gctttccacc gtgggggctt
    33541 tgatgctatc gacgtgcata tgagcgacct gctgactgga cgtacgggac tggaggattt
    33601 tcacgccctg gtcgcgtgcg gaggtttctc ctacggtgat gtgctgggtg ccggtgaagg
    33661 ttgggcgaag tctatcctgt tcaatgaccg tgtacgcgat gagtttgcaa ccttcttcca
    33721 tcgtccgcaa acgctggccc tgggggtatg taacggttgc cagatgatgt ctaacctgcg
    33781 tgaactgatc ccggggagcg agttgtggcc acgttttgtg cgtaacacct ccgatcgctt
    33841 tgaagcgcgt ttcagcctgg ttgaagtaac ccaaagcccg tctctgctgt tgcaggggat
    33901 ggtgggctcg caaatgccga ttgctgtctc tcatggtgaa gggcgcgtgg aagtgcgtga
    33961 tgcggcgcat ctggcggcac tggaaagcaa agggctggtg gcactgcgct atgtcgataa
    34021 cttcggcaaa gtcactgaaa cctacccggc taacccgaac ggttcaccga acggtattac
    34081 ggcggttacg actgaaagtg gtcgagtcac cattatgatg ccgcacccgg aacgtgtttt
    34141 ccgtactgtc agcaactcct ggcatccgga aaactggggc gaggatggcc catggatgcg
    34201 cattttccgc aatgcgcgta agcagttggg gtaagtcatc agttcattgg ttttgcagcc
    34261 cggatgcggc atttacgccg catccggcat taagcaccaa ccactatcat aagctcccaa
    34321 ctccgggagc ttttttgtgt ctgtaaatca cgacaatggg tggtttaccg tgtcgctttc
    34381 tggcgacact taaatgattg attttattgt aatctaataa gtttatctta agttgtctct
    34441 ttttagcgac acagtggctg atttacattc aaatagcgaa gaagccataa atccctcaaa
    34501 gcaacgtata atcagtacgt taccaaacta ttttctttat tggcacagtt actgcataat
    34561 agtaagcagt ggctcattca ccgacttatg tcagcccctt cgagacgtgc tacataaaat
    34621 tcgaatgacg cacaacaagg tgcctgccgt ccaacttctg atatcagcgt agctatatca
    34681 accatcgggc gaaacgtcga gttaggcacc gccttattcc ataacaaagc cgggtaattc
    34741 ccggctttgt tgtatctgaa cttcccctcg gttagcatca ggctattcgc gtctgacgag
    34801 agtaacacct tgaaacgctg gcccgttttt ccccgctcat tacgacaact ggtaatgctg
    34861 gcatttttgc taattctact acccctgttg gtgctggcat ggcaagcctg gcaaagcctg
    34921 aatgcgctta gcgatcaggc ggcgctggtt aaccgcacta cgcttattga tgcccggcgt
    34981 agtgaagcga tgaccaacgc ggcgctggag atggagcgta gctaccgtca gtattgcgtg
    35041 ctggacgacc ctacgcttgc gaaggtttat caaagccagc gcaagcgtta cagcgaaatg
    35101 ctcgatgccc acgcaggcgt actgccagac gataaacttt accaggcatt gcgtcaggat
    35161 ttgaacaatc tggcccaact tcagtgtaac aacagtggtc ccgatgctgc cgccgccgcg
    35221 cgtcttgaag cctttgccag tgccaatacc gaaatggtgc aggccacgcg cacagtggtg
    35281 ttctctcgtg ggcagcaact tcagcgtgaa atcgccgaac gtgggcaata ttttggttgg
    35341 caatcgctgg tgctatttct ggtgagtctg gtgatggtgc tacttttcac gcggatgatt
    35401 atcgggccgg tgaaaaatat cgagcgcatg atcaaccgtc tgggggaagg gcgttctctg
    35461 ggcaatagca tttcgttctg tggaccgagc gagttacgct cggttgggca acgtattctt
    35521 tggttaagtg agcgcctgtc atggctggaa tcccaacgcc atcaattttt aagacattta
    35581 tctcatgaat taaaaacgcc actggcgagt atgcgcgagg gcactgaatt actggctgat
    35641 caggttgtcg ggccgcttac gccagagcaa aaagaggtgg tgagcattct tgatagcagc
    35701 agccgcaatt tgcaaaaact gatcgaacaa ctgcttgatt acaaccgtaa acaggcggac
    35761 agtgcggtgg aactggagaa tgttgaatta gcaccgctgg tggagacagt ggtttctgct
    35821 catagcctgc ccgcacgggc taaaatgatg cataccgacg tcgatctcaa agcaacagct
    35881 tgcctggcgg agccaatgct gctgatgagc gtactggata atctttactc caatgcggtg
    35941 cactacgggg ctgaatccgg taacatttgc cttcgcagca gtttacatgg tgcgcgggtt
    36001 tatattgatg tcatcaatac aggcacgccc attccgcaag aggaacgcgc catgatcttc
    36061 gaaccctttt ttcagggaag ccaccagcga aaaggggcgg tgaagggcag cggtctggga
    36121 ttaagcattg ccagggattg tattcgccgt atgcaagggg aactgtatct ggtcgacgag
    36181 agcgggcaag acgtttgttt ccgcattgaa ttaccgtcgt cgaaaaacac gaaataatgc
    36241 taacagccta tcctatcagg ctattttatt tgtcagttcg gccccgggta gtgctcgaaa
    36301 tcttcacgta cgatatgtac gctccggttt ctccgcgctg tccatgtcca gactgtctgc
    36361 aacaattacg cccactggga taggctttaa gtctggtgaa tatgcgacac atttttcaac
    36421 gattattgcc ccgacggctg tggctggcag ggctgccatg tctggcattg ctgggttgcg
    36481 tgcagaatca caataagcca gccattgata cgccagcaga agaaaaaatt ccggtttatc
    36541 aactggctga ctatctttct actgaatgta gcgacatctg ggcgctgcaa ggtaaatcaa
    36601 ccgaaaccaa tccgctttac tggctgcggg cgatggattg tgctgatcgt ttaatgcctg
    36661 cgcagtcgcg tcagcaagct cgccaatatg acgacggcag ctggcaaaat acctttaaac
    36721 aagggatttt gctcgccgac gccaaaatta cgccgtatga gcgccgccag ctggtcgcgc
    36781 gtattgaagc gttaagtacc gagatcccgg cacaggttcg cccactgtat caactctggc
    36841 gcgacggtca ggcgctgcaa ctgcaactgg cggaagagcg ccaacgttac agtaaactcc
    36901 agcagtccag tgacagcgag ctggatacat tgcgccagca acatcacgtt ctacagcagc
    36961 aactggaact caccacccgc aagctggaaa acctgaccga tattgaacgc cagctctcga
    37021 cccgcaaacc ggctggaaat ttctcgccgg atacgccgca cgagagtgaa aagcccgcgc
    37081 catccaccca tgaggtcact cctgatgagc cataaacctg cgcatttatt attggtcgat
    37141 gacgatccgg gattgctgaa actgcttggc ctgcgcctga ccagcgaagg ctacagtgtg
    37201 gtcacggcgg aaagtggcgc tgaaggatta cgggtactga atcgcgaaaa agtagattta
    37261 gttatcagcg acctgcggat ggatgaaatg gacggtatgc agctatttgc tgaaatccag
    37321 aaagtgcagc cgggaatgcc agtaattatt cttactgcgc atggttctat ccccgatgcc
    37381 gttgctgcaa cacagcaggg cgtttttagt ttcctcacca agcctgtcga taaagatgcg
    37441 ctatatcagg caattgacga tgcgctggag caatccgcgc cagccaccga tgaacgctgg
    37501 cgcgaggcaa ttgtcacccg cagcccgctg atgctgcgtt tgctggaaca ggcgcggctg
    37561 gtggcgcaat cagacgtcag cgttttgatt aacggtcaga gcggcaccgg gaaagagatt
    37621 ttcgcccagg ctatccacaa cgccagcccg cgcaacagca aaccatttat tgctattaac
    37681 tgtggcgcat tgcccgagca attgctggag tcggagctgt ttggtcatgc gcgtggcgcg
    37741 tttactggcg ctgtcagcaa tcgcgaaggt ttattccagg cggcggaagg cggaacgcta
    37801 tttctcgatg aaattggcga tatgcctgcg ccgttacagg tcaaactgct gcgcgtgttg
    37861 caggagcgta aagtgcgccc gctgggcagt aaccgcgata ttgatatcaa tgtgcggatt
    37921 atttctgcca ctcaccgtga tcagtcaaaa gcgatggcgc gcggggaatt ccgcgaagat
    37981 ctctattacc gcctcaacgt tgtcagcctg aaaattccgg cactggcgga gcgcacagaa
    38041 gacattccgc tactggcaaa tcacctgttg cgccaggcgg cagagcgaca taaaccgttt
    38101 gtccgcgcgt tctctaccga tgcgatgaaa cgcctgatga ccgcgagctg gccgggtaat
    38161 gtgcgccagt tggtcaacgt gattgaacag tgcgtggcgc tgacctcatc tccggtgatt
    38221 agtgatgcgc tggtggagca ggcgctggag ggtgaaaata cggcgctgcc aacctttgtt
    38281 gaggcacgta atcattttga actcaactat ttgcgtaagc tgctgcaaat caccaaaggc
    38341 aacgtcaccc acgcggcgag aatggcgggg cgcaaccgga cagaatttta taaactgctt
    38401 tcccgacacg agctggatgc aaacgatttc aaggaatgaa ttggcgttat gtgttacgtt
    38461 tagcagatca aaagacaggc gaccttttca aggaatagca tgaaaaagat tgatgcgatt
    38521 ataaaaccct tcaagctgga cgatgtccgc gaagcactgg ccgaagtcgg tattaccggc
    38581 atgacggtga ccgaagtgaa aggctttggt cgccagaaag gccataccga gctgtaccgc
    38641 ggcgcggagt atatggtgga ttttctgccg aaagtgaaaa ttgagattgt cgtaccggac
    38701 gacattgtcg atacctgtgt cgataccatt attcgcacgg cgcaaaccgg caaaatcggt
    38761 gacggtaaaa tcttcgtctt tgacgtggct cgggtcattc gcatccgtac cggtgaagag
    38821 gacgacgcgg caatttaaac cgcgtcaagg gttgccggat gtttccatcc ggcaacatca
    38881 aattacagca ccttatgcgg gccaaagcat tcgtaatgaa tgttttcctg cttcacgccc
    38941 agatccacta actgtttcgc ggtaaactgc atgaagccaa ccgggccgca gagatagaac
    39001 tgcattgtcg gatcgctgaa cgcaccttcc agtttgctca aatccatcag accttcgcta
    39061 tcaaactgac ctttagcgcg atcggcttcg ctcggctgac gataccaggt gtgcgcggta
    39121 aagcgcggca gtgactgccc cagttcttta acttcatcag caaaggcgtg aacttcgcca
    39181 ttttctgccg catggaacca gttcacttgt gctgtgtggc ctgcttttgc cagcgtgtcg
    39241 agcattgcca gcattggcgt ttgaccaaca ccggtagaga ttaacgtcac tggtgtgtca
    39301 tctgcgacag ccataaagaa atcacctgcc ggagcgacca gtttcacgac atcgccaaca
    39361 ttggcgtgat tgtgcaacca gttggatacc tgcccaccct cttcgcgttt caccgcaata
    39421 cgatagcctt tgccatccgg tttgcgagtc aaagagtact gacgaatttc ctgatgcggg
    39481 aaaccttccg gcttcagcca gacgccgaga tattgccccg gacggtattc tgccactgcg
    39541 ccaccgtcga ccggctccag ttcgaagctg gtgataagcg cgctgcgcgg tgttttagcc
    39601 acaatgcgga aatcgcgagt accttcccaa ccaccggctt tgctggcgtt ttcgttatag
    39661 atttccgcct cgcgattgat aaatacatta gccagtacac cataggcttt accccacgcg
    39721 tccagcactt cctgccccgg gctgaacatt tcgtccagcg ttgccaacag gtgttcaccg
    39781 acgatgttgt actgttccgg tttgatctgg aagctggtgt gcttctgcgc gattttttct
    39841 accgctggca gcagcgcagg caggttttca atattactgg cgtaggcggc aatagcgtta
    39901 aacagggctt cacgttgatc gccattacgc tggttactca tgttaaaaat ttctttgagt
    39961 tctgggttat gagtaaacat acggtcgtag aaatgggcgg ttaactttgg ccccgtttcc
    40021 accagtaaag ggatggtggc ttttactgta gcgatggttt gagcgtcaag catatggtct
    40081 tccttttttg catcttaatt gatgtatctc aaatgcatct tataaaaaat agccctgcaa
    40141 tgtaaatggt tctttggtgt ttttcagaaa gaatgtgatg aagtgaaaaa tttgcatcac
    40201 aaacctgaaa agaaatccgt ttccggttgc aagctcttta ttctccaaag ccttgcgtag
    40261 cctgaaggta atcgtttgcg taaattcctt tgtcaagacc tgttatcgca caatgattcg
    40321 gttatactgt tcgccgttgt ccaacaggac cgcctataaa ggccaaaaat tttattgtta
    40381 gctgagtcag gagatgcgga tgttaaagcg tgaaatgaac attgccgatt atgatgccga
    40441 actgtggcag gctatggagc aggaaaaagt acgtcaggaa gagcacatcg aactgatcgc
    40501 ctccgaaaac tacaccagcc cgcgcgtaat gcaggcgcag ggttctcagc tgaccaacaa
    40561 atatgctgaa ggttatccgg gcaaacgcta ctacggcggt tgcgagtatg ttgatatcgt
    40621 tgaacaactg gcgatcgatc gtgcgaaaga actgttcggc gctgactacg ctaacgtcca
    40681 gccgcactcc ggctcccagg ctaactttgc ggtctacacc gcgctgctgg aaccaggtga
    40741 taccgttctg ggtatgaact tggcgcatgg cggtcacctg actcacggtt ctccggttaa
    40801 cttctccggt aaactgtaca acatcgttcc ttacggtatc gatgctaccg gtcatatcga
    40861 ttacgccgat ctggaaaaac aagccaaaga acacaagccg aaaatgatta tcggtggttt
    40921 ctctgcatat tccggcgtgg ttgactgggc gaaaatgcgt gaaatcgctg acagcatcgg
    40981 tgcttacctg ttcgttgata tggcgcacgt tgcgggcctg gttgctgctg gcgtctaccc
    41041 gaacccggtt cctcatgctc acgttgttac taccaccact cacaaaaccc tggcgggtcc
    41101 gcgcggcggc ctgatcctgg cgaaaggtgg tagcgaagag ctgtacaaaa aactgaactc
    41161 tgccgttttc cctggtggtc agggcggtcc gttgatgcac gtaatcgccg gtaaagcggt
    41221 tgctctgaaa gaagcgatgg agcctgagtt caaaacttac cagcagcagg tcgcgaaaaa
    41281 cgctaaagcg atggtagaag tgttcctcga gcgcggctac aaagtggttt ccggcggcac
    41341 tgataaccac ctgttcctgg ttgatctggt tgataaaaac ctgaccggta aagaagcaga
    41401 cgccgctctg ggccgtgcta acatcaccgt caacaaaaac agcgtaccga acgatccgaa
    41461 gagcccgttt gtgacctccg gtattcgtgt aggtactccg gcgattaccc gtcgcggctt
    41521 taaagaagcc gaagcgaaag aactggctgg ctggatgtgt gacgtgctgg acagcatcaa
    41581 tgatgaagcc gttatcgagc gcatcaaagg taaagttctc gacatctgcg cacgttaccc
    41641 ggtttacgca taagcgaaac ggtgatttgc tgacaatgtg ctcgttgttc atgttggatg
    41701 cggcatgaac accttcttag gcctacaaaa tcttgcaaat tcaatatatt gcaatctccg
    41761 tgtaggcctg ataagcgtag cgcatcaggc aatttttcgt ttatgatcat caaggcttcc
    41821 ttcgggaagc ctttctacgt tatcgcgcca tcaaatctgt cgtaactgcg cctcaacata
    41881 caaatagcca attcccagca cctgttgtgc gcggcttaat tgcccgaagc caatttgcgt
    41941 cgctttcacg ctcggtcctt cgtcgcggtc gaacgggtta aatcctgtct ggcgaataat
    42001 agtattaagc cagttctcac caaaggcaca actgcgaccg tacaaccaaa tctgattaat
    42061 attgaggatg ttgaggaagt tatacagact caaaccaatg gcattggcag agcgatcaac
    42121 ccagctggtg atccacggtt ctccactctg ccaggcagcg attaactgcg cggttgtcag
    42181 tttttcagga tcaagctgag tattaaccgg ttgtgatttt agccataccc gcgcctgttt
    42241 ttttaatgca cttaacgagg cgacggtttc caggcagccg taacgtccac agtcgcagac
    42301 gacgccatcc ggattaacga tggtgtgacc aatctgtccg ctaccataca aactgccgtg
    42361 ataaatttgc tcgttaatca cgaacgacga gccaatgccg taatcaacgt tgatcacgca
    42421 gaaatcccgt tcctgcgaat tattttgcca tttctccgcc agcgccagca tcacgcagtc
    42481 attatcgacc agcacccgaa tgcccagctt ctcttccagc agatacttca cctcaactgg
    42541 cgttgtccac ggcgcttgcg gcatggtttg cgatacgccg gtcaccggat caacctgccc
    42601 gtggattgcc agcgccagat tgatggtgcg gtccggccac aatttacggt ggcgatgcca
    42661 gcatttttcg atttcggaca gcagcgcctg cggcgtcggt acatcaatct gtaaatactc
    42721 aaattcgcct ttcggactta aacaagcatt agcaacctga cactcaatac tggtgggcgt
    42781 cacgttcagg cacagcgtcc agtcaccttc cggcgcaatc agccatgtac cgctactatg
    42841 cccgcgcgtc tggctttcat cgtcaatatt caccacccgt ttttcgcttt ccagttcctg
    42901 caaaatatta ctgactgccg gaatcgaaat ttgcgccagc cgggccagcg ttgatttatt
    42961 ggcgcgcttt tgccggtaca gcagttccag aatcacgcat ttgttatggt ggcgaatctg
    43021 ttgattgtta atgcaggctc tcatcttcac taaaccttgt ttagttaatt gcgtgattat
    43081 tccgcgaaaa cattgccaga ttgttcaata cactgccaca aatcttttaa ttcagtatgt
    43141 cttgttaata ttgagggcac catgactcca gtaaaagtgt ggcaagagcg cgttgagatc
    43201 ccgacctatg aaaccgggcc gcaggatata catcccatgt tcctggaaaa tcgcgtttat
    43261 cagggatcgt ccggcgcggt ttatccctac ggcgtgaccg atacgctgag cgagcagaaa
    43321 accctgaaat cctagcaggc ggtgtggctg gaaaacgact acatcaaagt gatgatcctg
    43381 ccggaactgg gcggtcgggt gcatcgcgca tgggataaag tgaaacagcg cgattttgtc
    43441 tatcacaatg aagtcattaa acctgcgctg gtggggctgc tgggaccgtg gatctccggc
    43501 gggattgagt ttaactggcc gcaacaccat cgcccgacca cctttatgcc cgttgatttc
    43561 accctcgaag cccatgaaga cggtgcacag acggtgtggg tcggcgaaac ggagccgatg
    43621 catggtttac aggtgatgac aggtttcacc ctgcgccctg accgggcggc actggaaatc
    43681 gccagccgcg tctataacgg caacgccacg ccgcgtcatt tcttgtggtg ggccaacccg
    43741 gcagtgaaag ggggtgaagg gcatcagagc gttttcccac cggatgtgac tgcggtgttt
    43801 gatcacggca aacgggccgt ctccgctttc cccatcgcca cttactacaa agtggactac
    43861 tccgccggag tggacatttc tcgctataaa aatgtgcctg ttccaacctc atatatggcc
    43921 gaaaaatcgc agtacgattt tgtcggcgcg tggtgtcacg atgaagatgg cggtttgctg
    43981 cacgttgcca accaccatat tgcgccaggt aaaaaacagt ggagttgggg acacagtgaa
    44041 tttggccagg cgtgggataa gagtctgacc gacaataacg gcccgtatat cgaactgatg
    44101 accggtattt ttgccgataa ccagcctgat tttacctggc ttgatgctta cgaagagaag
    44161 cgtttcgagc agtatttcct gccttatcat tctctgggca tggtgcaaaa tgcctcccgc
    44221 gatgcggtga taaaactcca gcgtagtgag cgggggattg agtgggggct gtatgccatc
    44281 tctccgttga acggataccg tctggcgatc cgcgaaatcg gcaaatgcaa cgcgttgctc
    44341 gatgatgccg tggccctgac accagcgacc gccatctggg gcgtgttgca cggtatcaat
    44401 cctgacagac tgaccattga actctccgat gccgacggca atattgtact gagttatcag
    44461 gaacatcagc cgcaggagtt gccgttgccg gacgtcgcca aagcgccact ggcagcacaa
    44521 gacattacca gtacagatga agcctggttt atcggtcagc atctggagca atatcatcac
    44581 gccagccgtt caccgttcga ttactacctg cgcggcgtgg cgctggaccc gctggattat
    44641 cgctgtaacc tggcgctggc gatgctggaa tataaccgcg cagatttccc gcaagcggtg
    44701 gcgtatgcca gtcaggcgct gaaacgcgca catgcgctga acaaaaaccc gcagtgtgga
    44761 caagcaagtt tgattcgcgc cagtgcttac gaacgtcagg gacaatatca acaagccgaa
    44821 gaggatttct ggcgtgcggt ctggagcggc aatagtaaag caggcggcta ttatggtctg
    44881 gcacgactgg cggcgcgtaa tgataacttc gacgcgggtc tggacttttg ccaacaaagt
    44941 cttcgtgcct gcccaaccaa tcaggaagtg ctttgcttgc ataatctgct gctggtgtta
    45001 agtggtcgtc aggacaacgc gcgtttgcag cgcgagaaac tgctgcgtga ttatccgctg
    45061 aacgccactc tgtggtggct gaactggttc gatggtcgta gcgaatcagc cctcgcgcag
    45121 tggcgcggtc tgtgtcaggg acgcgacgtt aacgctctga tgaccgccgg gcaactgatt
    45181 aactggggaa tgcccaccct ggcggcagag atgctgaacg cactggactg ccagcgcacg
    45241 ctgccgcttt acctgcaagc cagcttgctg ccgaaagccg aacgtggcga actggtcgca
    45301 aaagccattg atgtcttccc gcagtttgtc cgtttcccga atacgctgga agaagtggcg
    45361 gcgctggaga gtattgaaga gtgctggttt gctcgccatt tactggcctg cttctactac
    45421 aacaaacgta gctacaacaa agccattgcc ttatggcaac gttgcgtaga gatgtcgccg
    45481 gagtttgccg acggctggcg cgggttagcg atccatgcgt ggaataagaa acacgattat
    45541 gagctggccg cgcgttatct tgataatgct tatcagcttg cgccgcagga tgcacgtctg
    45601 cttttcgaac gggatttgct ggataagtta agtggagcca caccggagaa acgactggcg
    45661 cgtctggaaa ataatctgga aattgcgctg aaacgcgacg acatgaccgc agaactgctc
    45721 aatttgtggc atctcacggg tcaggcagac aaagcggcgg acattctcgc cacgcgtaaa
    45781 ttccacccgt gggaaggcgg ggaagggaag gtcaccagtc agtttatcct caaccagtta
    45841 ttacgcgcct ggcagcatct tgatgccaga gagccgcagc aggccagcga actgcttcat
    45901 gccgcgctgc attatccgga gaatttaagc gaaggccgtt taccggggca aactgataac
    45961 gacatctggt tctggcaggc gatatgcgcc aacgcgcagg gcgatgaaac tgaagcgatg
    46021 cgttgtttac gtctggcggc gaccggcgat cgcaccatta acatccacag ttattacaac
    46081 gatcagccgg ttgattatct cttctggcaa ggaatggcgc tgcgactgct gggtgaacag
    46141 caaaccgcac agcaactgtt tagtgaaatg aaacagtggg cgcaagagat ggcgaaaacc
    46201 agtattgagg cggatttctt tgctgtttca caacctgacc tgttatcgct gtatggcgat
    46261 ttacaacagc agcataaaga aaaatgcctg atggtggcga tgctggcgtc cgcgggactc
    46321 ggggaggttg cgcaatatga gtctgctcgc gctgaattga cggcgattaa tccggcctgg
    46381 ccgaaagcgg cattattcac cactgtgatg ccttttattt ttaactacgt tcactaaacc
    46441 ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata attagaataa
    46501 gccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag aacaacccgt
    46561 aatctattat taatggctac gctattaggt agcgcactat ttgccagggc tgcggataaa
    46621 gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg agtgcgtcag
    46681 ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga aactaacgcc
    46741 cagggtgata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg taatgtcgat
    46801 gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg tcgcgccagt
    46861 gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg tgtcgataaa
    46921 tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact gggtaacgct
    46981 gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt catcaattgc
    47041 ga
//
LOCUS       DADKXM010000017        40560 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.017, whole genome shotgun sequence.
ACCESSION   DADKXM010000017 DADKXM010000000
VERSION     DADKXM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.017"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..956)
                     /locus_tag="JQR05_000960"
     CDS             complement(<1..956)
                     /locus_tag="JQR05_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY5323178.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGS"
     gene            1203..1829
                     /locus_tag="JQR05_000961"
     CDS             1203..1829
                     /locus_tag="JQR05_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY5323179.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNSNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2941)
                     /locus_tag="JQR05_000962"
     CDS             complement(1826..2941)
                     /locus_tag="JQR05_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY5323180.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JQR05_000963"
     CDS             complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JQR05_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY5323181.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3648..4973
                     /gene="rimO"
                     /locus_tag="JQR05_000964"
     CDS             3648..4973
                     /gene="rimO"
                     /locus_tag="JQR05_000964"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY5323182.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5020..6348)
                     /locus_tag="JQR05_000965"
     CDS             complement(5020..6348)
                     /locus_tag="JQR05_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5323183.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6356..8704)
                     /locus_tag="JQR05_000966"
     CDS             complement(6356..8704)
                     /locus_tag="JQR05_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5323184.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEVIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8882..9793)
                     /gene="gsiD"
                     /locus_tag="JQR05_000967"
     CDS             complement(8882..9793)
                     /gene="gsiD"
                     /locus_tag="JQR05_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY5323185.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLTGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9796..10716)
                     /gene="gsiC"
                     /locus_tag="JQR05_000968"
     CDS             complement(9796..10716)
                     /gene="gsiC"
                     /locus_tag="JQR05_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY5323186.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSIVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10734..12273)
                     /gene="gsiB"
                     /locus_tag="JQR05_000969"
                     /pseudo
     CDS             complement(10734..12273)
                     /gene="gsiB"
                     /locus_tag="JQR05_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(12293..14164)
                     /gene="gsiA"
                     /locus_tag="JQR05_000970"
     CDS             complement(12293..14164)
                     /gene="gsiA"
                     /locus_tag="JQR05_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY5323187.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEYAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCEVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14151..15116)
                     /gene="iaaA"
                     /locus_tag="JQR05_000971"
     CDS             complement(14151..15116)
                     /gene="iaaA"
                     /locus_tag="JQR05_000971"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY5323188.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15320..16555
                     /gene="moeA"
                     /locus_tag="JQR05_000972"
     CDS             15320..16555
                     /gene="moeA"
                     /locus_tag="JQR05_000972"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY5323189.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16555..17304
                     /gene="moeB"
                     /locus_tag="JQR05_000973"
     CDS             16555..17304
                     /gene="moeB"
                     /locus_tag="JQR05_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY5323190.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17483..18145)
                     /gene="fsa"
                     /locus_tag="JQR05_000974"
     CDS             complement(17483..18145)
                     /gene="fsa"
                     /locus_tag="JQR05_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY5323191.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18276..19175
                     /locus_tag="JQR05_000975"
     CDS             18276..19175
                     /locus_tag="JQR05_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY5323192.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHRKKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLPAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19181..21613
                     /locus_tag="JQR05_000976"
     CDS             19181..21613
                     /locus_tag="JQR05_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY5323193.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCCFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21759..22574
                     /gene="ybiV"
                     /locus_tag="JQR05_000977"
     CDS             21759..22574
                     /gene="ybiV"
                     /locus_tag="JQR05_000977"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY5323194.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22726..23991
                     /locus_tag="JQR05_000978"
     CDS             22726..23991
                     /locus_tag="JQR05_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY5323195.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24232..25824)
                     /locus_tag="JQR05_000979"
     CDS             complement(24232..25824)
                     /locus_tag="JQR05_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY5323196.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26043..26963
                     /gene="ldtB"
                     /locus_tag="JQR05_000980"
     CDS             26043..26963
                     /gene="ldtB"
                     /locus_tag="JQR05_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5323197.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27021..28139)
                     /locus_tag="JQR05_000981"
     CDS             complement(27021..28139)
                     /locus_tag="JQR05_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY5323198.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAVGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28136..28603)
                     /locus_tag="JQR05_000982"
     CDS             complement(28136..28603)
                     /locus_tag="JQR05_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY5323199.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28789..28917
                     /gene="mntS"
                     /locus_tag="JQR05_000983"
     CDS             28789..28917
                     /gene="mntS"
                     /locus_tag="JQR05_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY5323200.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29189..30775
                     /locus_tag="JQR05_000984"
     CDS             29189..30775
                     /locus_tag="JQR05_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY5323201.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETSFVQSKETSCYL
                     YTMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQV
                     PFMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITN
                     FQLQKVDYNHLGTDIFDPKP"
     gene            complement(30824..31339)
                     /gene="ompX"
                     /locus_tag="JQR05_000985"
     CDS             complement(30824..31339)
                     /gene="ompX"
                     /locus_tag="JQR05_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY5323202.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31392..31457)
                     /locus_tag="JQR05_000986"
     CDS             complement(31392..31457)
                     /locus_tag="JQR05_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323203.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31692..32579
                     /gene="rhtA"
                     /locus_tag="JQR05_000987"
     CDS             31692..32579
                     /gene="rhtA"
                     /locus_tag="JQR05_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY5323204.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32878..33381
                     /gene="dps"
                     /locus_tag="JQR05_000988"
                     /gene_synonym="pexB"
     CDS             32878..33381
                     /gene="dps"
                     /locus_tag="JQR05_000988"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY5323205.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33785..34531
                     /gene="glnH"
                     /locus_tag="JQR05_000989"
     CDS             33785..34531
                     /gene="glnH"
                     /locus_tag="JQR05_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY5323206.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            34670..35329
                     /gene="glnP"
                     /locus_tag="JQR05_000990"
     CDS             34670..35329
                     /gene="glnP"
                     /locus_tag="JQR05_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY5323207.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35326..36048
                     /gene="glnQ"
                     /locus_tag="JQR05_000991"
     CDS             35326..36048
                     /gene="glnQ"
                     /locus_tag="JQR05_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY5323208.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVIQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36309..38534
                     /gene="ybiO"
                     /locus_tag="JQR05_000992"
     CDS             36309..38534
                     /gene="ybiO"
                     /locus_tag="JQR05_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY5323209.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(38531..39457)
                     /gene="rlmF"
                     /locus_tag="JQR05_000993"
     CDS             complement(38531..39457)
                     /gene="rlmF"
                     /locus_tag="JQR05_000993"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY5323210.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39733..39993
                     /locus_tag="JQR05_000994"
     CDS             39733..39993
                     /locus_tag="JQR05_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5323211.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40258..>40560
                     /locus_tag="JQR05_000995"
     CDS             40258..>40560
                     /locus_tag="JQR05_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY5323212.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10080 a        10732 c         9877 g         9871 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaacccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atcggccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaactcta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga
     2041 atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa
     2101 caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat
     2161 agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa
     2221 tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc cacggattca
     2281 tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt
     2341 cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc
     2401 cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa
     2461 aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt
     2521 ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc
     2581 ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc
     2641 agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc
     2701 cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc
     2761 agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg
     2821 ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag
     2881 ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca
     2941 taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat
     3001 ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt
     3061 ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gttaagctca tcagacaatt
     3121 ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa
     3181 acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg
     3241 atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa
     3301 gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg
     3361 cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg
     3421 tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg
     3481 gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt
     3541 acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg
     3601 tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa
     3661 ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag
     3721 agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc tatgacgatg
     3781 cggacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg
     3841 aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg
     3901 caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata
     3961 gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat
     4021 tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga
     4081 aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg
     4141 acctggtgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg
     4201 gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac
     4261 atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac
     4321 agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg
     4381 acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc
     4441 agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac
     4501 tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta
     4561 ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac ttcctgaaag
     4621 aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca
     4681 atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac cgtttcatgc
     4741 agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg
     4801 tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg
     4861 aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc
     4921 gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc
     4981 aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgtgc
     5041 cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac
     5101 ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa tgcttcccac
     5161 ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc cgccagtata
     5221 aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta ccgggatctg
     5281 tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt
     5341 gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc ctttttccgg
     5401 cagcgctttt aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag
     5461 gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc
     5521 gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg
     5581 attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg ccaccaatgc
     5641 ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat acgcaaatac
     5701 gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc
     5761 gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat
     5821 caggttctcg ctgtccatcg ccagcgattt tgacagtaaa gaaaaacgct ccagaccaag
     5881 caaaaatatc agactacgat tgtgagttac cggatagagg taatagacga aggttttacc
     5941 gtcaaaagag ataaaccctg tatttttgac atgtcgatac ttcacaacct gcataatatc
     6001 tgcacaataa tttttctcga actcctttac ctgaatacat tcgtcactgg aaatcagata
     6061 aggaccgaca tcgtacaaat aagaggggga aaccgagtcg gcgtaagctg tgcgcaaacc
     6121 aaccaacagc tgttcctccg tgggatttag cgtgttgata agatcaattg ccacgctacg
     6181 ccgatattca gcaatatttg ccaccgcaaa agcagtatca ataacggact gtttcaggct
     6241 atacacccgt tcctttacca tttgagtgta gatcccgcag gcaactgctg aaatcatgat
     6301 aaaaatcagc agactgactg taagtacgaa cttattgatt ctggacatac gtaaactact
     6361 cttttactaa ttttccactt ttatcccagg cggagaatgg catgggtttc tggtacagat
     6421 acccctgagc ctgaacacat cctaatttaa ttaattcgcg cgcaacgtct tcgctctcta
     6481 cgccttccgc aatcaccggg atccggttat agcgtgccag atcgaaaata atgcgcacaa
     6541 tttttcccgc agtaccgaaa atatcgccgg taaattgcga gtccaccttt atgctgtctg
     6601 gattaatttc ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca tcaatcgata
     6661 tccgtacccc ttgtttacgc agttgttcaa tattgagata aaaatgttca ttaattcgct
     6721 cgatcacggt ttcagtgatc tcaacacaga ggctgttcgc cttcaggcca ttttctttca
     6781 tgatggtggt aaaacgctca tgaaagtgcg gttcgtttaa ctgccagggc gaaacattaa
     6841 tatgcagtaa gaaatcatcc ttcagcgacg cacgttgact aaaatgtctg aattcaccca
     6901 ttgccgtttt aataatctga taacccagct cattgataaa cccgatatct tcagcaatgg
     6961 taatgaactt cagcggtgaa atgatccccc gctccgcaga aacccaacgg cacaacgcct
     7021 ccgcgctcag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg aggaatagtt
     7081 cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct tctatttcgt
     7141 tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg atactggaaa
     7201 tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa ggttcacaaa
     7261 tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa tcgtttacca
     7321 tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa tagagatcgg
     7381 cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc tgacacaatt
     7441 tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacggct tccagaccac
     7501 acagtttgat gatccccaca gccagatact gattattgtt ggactcaata atcgccatat
     7561 ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta tttgaccgtt
     7621 caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc tctttcgcat
     7681 cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa aacgccagcg
     7741 tattaaccag ctcggtcatt tcagaaaatt gccgacggaa cacacgagtg gtcatgaaat
     7801 ataccaccag caacacgata ataatgaacg caaacggtag ataaaaaagt ttatctgatg
     7861 cgttcaataa attaccgcca ggataataaa aatagatttc gaacaatgca ttatttggca
     7921 ctgctgatgt ctttgcaata tactccgtat cgacagaatc aataatatta taattacctt
     7981 cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg acctcatgac
     8041 cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac tcgttcagat
     8101 accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga tctttgccgt
     8161 tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa ttgtatgccg
     8221 gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc gatgggttaa
     8281 aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact tgttcctgat
     8341 gataaacaac gattttatca ttcgccctcc gcgaaaagag ataatattta ttatcatcaa
     8401 gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc acggcgcgtt
     8461 gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg gccgaacgtt
     8521 caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac
     8581 gttttaccgc gcgttccgat accagttgat agttaattat gaaaaaaaga ccaataaatg
     8641 acagtgctgc caataaaaat aaaattatca gccttatctt tatcttttcg tataaactca
     8701 gcatgttaat acatttaaca aaataactat ctgaacgctg tgccgctgca aaaatagtcc
     8761 cttgctgcgg ctttttaacc aaattacccg attctacaaa atcagcagaa agttacaaca
     8821 cttgcttagg gcaataattt cctgtgaggt gattaccctt tcaagcaata ttcaaacgta
     8881 actatccttt aattttcgga tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg
     8941 ccagtacggt cagaaaaata gccagggccg gaaaaacagc gacatgcggc gcgataacca
     9001 tatccgctcg tgcctcattg agcattgctc cccactctgg tgtcggcggc tgcgcaccga
     9061 ggccgagaaa tgacaggctg gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa
     9121 acaccacgat agaagagacg gtcccaggca ggatatgacg caataaaatg gtcatatcgc
     9181 tggcaccaat actgcgtgct gactcaataa aggtttgctg tttcaacacc agcgtgttgc
     9241 cgcgcaccag gcgggcaaac gcggggatgg aaaaaatggc gactgcgata atcacgttag
     9301 caatgccgct tcccaacacc gcaacaacag cgatcgccag taaaataccc gggaaggcaa
     9361 acagcacatc gcaaatgcgc atgatcagcc gatcccacca gccttcataa tatccagtca
     9421 acaagcccag caacgtccct atcgccacac cgataaacac ggcaaacacg cccgccgcca
     9481 gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc
     9541 caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa taattttcgg
     9601 cgtcataggg agcgatccag cgtgcaaaaa tggccaccac aatcaataaa ataacgaata
     9661 atgcggcggt catcgccata tgctgacggc gaaatcgtcg ccagaattca tgccacggtg
     9721 tacgtacctg gtcaggtttg accagtggca tggcgtttaa caccgcctga cgtcgccagt
     9781 taaatagtcg catccttact tgtacctgat agccgggtta atggcggcgt aaagcacatc
     9841 caccactaag ttgataagaa taaattccag cgagaaaagc agaatttccg cctgaatcac
     9901 cgggtaatcg cgcatttcta cagagtcaac gagtaagcgt ccaagtccgg gccagttgaa
     9961 aactttctcc accacgatag aaccaccgag caaaaagcca aactgtaagc ccatcatggt
    10021 cactaccggg atcatcgcgt tgcgtagccc gtgtttgagg acaacccagg tttcgctcac
    10081 ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa acgtcgacaa atgacgcacg
    10141 ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc gtcagggagg gtaaaatgta
    10201 gtgctgccaa ctgtctgctc ccacagtagg cagccagccc agttcaacgg agaatacctg
    10261 aattaaaagc atccccagcg caaatgccgg aaacgagatc cccgacaccg caatggtcat
    10321 actcaatcga tccggccaac gattacgcca gacggcggcg ataattcccg ccgccatacc
    10381 aaatatcacc gcccagacta tactggttat ggtcagccac agcgttggca taaagcggct
    10441 ggcaatctca tcggcaaccg gacgacgcga caccatcgac aggccaaaat ccccctgcac
    10501 agcattgctg atatagtgcc agaactggtg atacagcggc tgatccaacc ccagctgctg
    10561 acgcaccagt tctataacct gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc
    10621 gccgggcagc atgtggacaa ataaaaacac cagcaccgag acgataaaca gcgtcggaat
    10681 caaccccagt aatcgtttga taacgtaatt aagcattcca ctccctgcgt tgcttattgc
    10741 aaatccgcgt cttcaaagct gaagccggtg tctggcatga tccaaaaacc ggtcaggttt
    10801 ttactgtgtg ccgacaccag tttttctacc accagcggga tccacggcga ttcttgccag
    10861 atgatatcct gcgccgcctt atataagcgg gtcttttccg ccggatcatt cgttttcagt
    10921 gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa acgcggtatt aaacagcgtc
    10981 ggtggccagt tttgcgaggc aaacagcggc gatagtgccc agtcggcttc gccggttgaa
    11041 gccgaccagc cagtgtagaa catccgcacg ccgctctctt tttgcccttt accttcaact
    11101 tctgccgccc gctgtccggc atccatcgcg gtcacctggg ctttaatccc gacctgcgct
    11161 aactgctgct gggtaaattg cagcactttc tgcgcggtgc tgtggttatg tgacgaccac
    11221 agcgtggtac tgaaaccgtt gggatatccc gcctctttca gtaattcgcg cgctttcact
    11281 ggatcgtaag gccacggttt ataactttgc gcgtaggcga tacttggcgg taccacacca
    11341 gtagctggcg ttgcatagcc cgcaaaggca actttcacca gcgccggacg gttaatggcg
    11401 taattcagcg cctcacggac cttcgggtta tcgaacggct tttgcgtcac gttcatactg
    11461 atataacgct gcataattga cggactggcc atcaactcga tatttttgtt tttctccagc
    11521 agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt
    11581 gccgcgcggg tgttgttatc cgccaccgga cgccaggtta tgctgtccag tttgggcaat
    11641 cctggctgcc agtaacccgc gaattttttc accttcacaa aatcggtctg attccaggta
    11701 tccagttcat actggtccgg ttcccaccgg atgaaaacca atctccttgc catatttttc
    11761 cagcgctgtc ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga
    11821 gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg ttttggcaat
    11881 attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt
    11941 cgcttttacc gccactgcgt tgaaatcggt gccatcttgg aatttaattc cttcccgcaa
    12001 tttcacggtg taagtaatgc cgtcatcgga aacggtataa ctctccgcca gcacgttttt
    12061 cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg
    12121 agataacgtg tcattcgcgt catacggatc gagcgtggtg aaattcgatc ctaccgccac
    12181 caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc
    12241 cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac
    12301 gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca
    12361 acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca
    12421 gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt
    12481 tacgcgtata aggatgctgc gggttttcga agacctcgca ccgtgaacca atttcaacaa
    12541 tttgcccgag atacatcacc gccacacgat gactaatccg ctctaccaca gccatatcgt
    12601 gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa
    12661 tctgcccgcg aatagaaaca tccagcgccg aaacggcttc gtcggcaatg atcacttttg
    12721 gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat
    12781 gcgggtaacg ccaggcatat tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc
    12841 gtgcagccgc gtctttacct ggcaataaac cgtgtacacg cagcggttcg ataatcgaat
    12901 caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat
    12961 cccggcgcaa tgcctgaagt ttgccaggtg acaaggtatc gattcgctga ccgttaaaga
    13021 taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac
    13081 cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt
    13141 tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac
    13201 gggtgacaag attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga
    13261 tggggggttc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat
    13321 ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat
    13381 gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg ccctgataca
    13441 tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa
    13501 cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag
    13561 tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg
    13621 ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgaa taacgtgaaa
    13681 gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg
    13741 cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag
    13801 taaatactgg gttcagcgat gtcatcggct cctgaaatat catcgccata tccgcaccgc
    13861 gcacatggcg catttgtgca gcgctctgct cgctaagttc aatcacatca cgactgcgcc
    13921 gccgcaacag cattttatcg cactgtacta aaccgcccgc ctgttccaac agacgcatca
    13981 atgccagcgc agtcactgac ttaccggagc cggattcgcc tacaattgcc agcgtctcac
    14041 cgcgttgcag actaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa
    14101 aggcaatatt aagattttca accgccagca cattaccggc atcaagttca tcactgtgtg
    14161 gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc
    14221 caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattcccttc atggtcgata
    14281 gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag
    14341 gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc
    14401 gcgcggatga agacttcgcc tgtgccggta caagaaaccg ccacactggc gttattggcg
    14461 tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt
    14521 ccgcctgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg
    14581 gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct
    14641 tcctcgcgcg ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag
    14701 acgcgctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca
    14761 tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg
    14821 cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg
    14881 gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc
    14941 agacgcaccg cttccgtcac cacatccagc gcactttcgc cggcttccag cattttctgc
    15001 ccggtttcaa caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc
    15061 tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta
    15121 ttcgtccatt aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac
    15181 tcattcaatt tttgaatata gaaagatgga tatggcatgt aaaggcagga gccaacgcta
    15241 cgcccgaggt ggcatacagt ctatggcgtt tttctgacat aataggcaaa ttcgattttg
    15301 cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct
    15361 taatgagatg ctttctcgcg ttaccccact gaccacccag gaaacgctgc cactggttca
    15421 gtgttttggt cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga
    15481 taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct
    15541 gcccgttgcc ggtaaatcct ttgccggtca gccataccat ggtgaatggc ctgcgggtac
    15601 ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca
    15661 ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca
    15721 aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcgggaac
    15781 tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt
    15841 gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca
    15901 gccgctgggc gacggccaaa tctacgacac caaccgtctc gccgtacacc tgatgttaga
    15961 acagctgggt tgcgaggtaa tcaacttagg gattatccgc gacgatccac atgccctgcg
    16021 cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc
    16081 agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg
    16141 gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt aaactcagca atagctggtt
    16201 ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc
    16261 tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg
    16321 cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca
    16381 acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga catcagggtt cacatatatt
    16441 tagctccttt agtctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga
    16501 cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg
    16561 gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat
    16621 tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct cggtggactc
    16681 ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac
    16741 ttcgacacgg tttcgctctc gaatctgcaa cgccagacac tgcacagtga tgccacggtc
    16801 gggcaaccga aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg
    16861 attacgccag tcaatgcact gctagatgac gcagaacttg cagcattgat tgctgaacac
    16921 gatctggtgc tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt
    16981 tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg
    17041 gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag
    17101 aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt aattggttcg
    17161 ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa
    17221 atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg
    17281 gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc tgataagacg cgacaagcgt
    17341 cgcatcaggc atttgctcca gacgccagat gcggcgtgaa cgccttatct ggcctacggt
    17401 tcgggcacag atttccaagc gcggcaggca tcggtgcact ccgccacgtc atcagcaatt
    17461 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc tgccagtcct
    17521 gctcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat
    17581 ccagtggcag agtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg
    17641 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
    17701 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
    17761 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca tataccgcgg
    17821 ttcccagcgt cggaatccct tccgctttta acatcttaat cgctgccagc ccctcggctg
    17881 tcaccggaac tttcaccacg atatccgcaa taatagaacg cagcttaagc gcgtcattca
    17941 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
    18001 cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg
    18061 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt
    18121 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
    18181 gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa
    18241 gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
    18301 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
    18361 gccgctggtg tcagaacccg gaaagccgcg cccgtacgca ggatctgctg tatgacgcac
    18421 gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg
    18481 cgctgaatgg tttgcttatt catcggaaaa agttaacccc ggagcatctg acggcgttaa
    18541 ccgactgctg tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga
    18601 tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggcttaacgc
    18661 tttcgggtgg tgaacccttc atgcagccgg aaatggcgac ggcgctgctg caagcctgcc
    18721 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg
    18781 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc
    18841 cgtttaaaca gtggaccgac ggtaacgccg ccagggtgct ggataacctg aaaaaactcc
    18901 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
    18961 aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
    19021 attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
    19081 acgccccgga aaaaccgctt gatgcgccag agctgctcga ttttgcccag cagtatgcct
    19141 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
    19201 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
    19261 agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
    19321 gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat
    19381 caaacacgat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt
    19441 cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
    19501 tgctggcttt gcggtgagcg aagagaacaa acgtgttctg catgaagtgt gcccgtggtg
    19561 gcgcggtcag acggtacagg atcgctgcta tggcatgttt accgatgagc aaaaaggtct
    19621 gctggcgacc ggcatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
    19681 ggtcaatttc ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga aagtagcgga
    19741 acgtcgctcg cgcatcaatc tgacggtgct ggaagatttg cacggcgaac agttcctgaa
    19801 agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc
    19861 gcgtgcaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatggc
    19921 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
    19981 gtgttacttc atccagttga ttttgcagat tgaatctaac ggtcactcgg tgtcgtttgg
    20041 tcgtatggat cagtatctct acccgtacta tcgccgcgac gttgaactga accagacgct
    20101 ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ttgaaattgc tggaagtgaa
    20161 caaaatccgc tccggctccc actcaaaagc ctctgcggga agtccgctgt atcagaacgt
    20221 caccattggc ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga atccactctc
    20281 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
    20341 ttaccatgca ggaatgagta acgatttcct cgacgcctgc gtacaggtga tccgctgctg
    20401 cttcgggatg ccggcattca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
    20461 tattgaaccg caggacgctt atgactacgc agcgattggt tgtatagaaa ccgccgtcgg
    20521 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
    20581 ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa
    20641 agcgttgtcg gcaggtaact tcaacaactt cgatgaagtg atggacgcgt gggatacgca
    20701 aatccgttac tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
    20761 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
    20821 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg tcggtattgc
    20881 caacctcggc aacagcctgg cggcagtgaa gaaactggtg tttgaacagg gcgcgattgg
    20941 tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact
    21001 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ccgtcgatac
    21061 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataacccacg
    21121 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta tctccgctaa
    21181 cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct
    21241 ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg
    21301 ctcagtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
    21361 gaatccggca acgctggaga acgaatctga caagcagaaa ctgatgatcc tgctgcgtac
    21421 cttctttgaa gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
    21481 gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtacgtg tcgcgggtta
    21541 ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg cccgtactga
    21601 acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca
    21661 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
    21721 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
    21781 cagatatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
    21841 cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc ggtaatcagt
    21901 attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
    21961 acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg
    22021 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
    22081 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
    22141 aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac gtactgttca
    22201 agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa ctacacatag
    22261 cgctggatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc
    22321 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
    22381 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
    22441 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
    22501 atgataataa tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat aacacatccc
    22561 cttttaacag ctgacctctc taccggagcc atgcgctccg gttttccctt ctttttacat
    22621 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt gtattgtcac
    22681 atgcaaatca gcatagtttc gaatgaaagt taatgcgagg tggttatggc ttctactttt
    22741 accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
    22801 cgggcgcagc ttggcgatgt ccagcagatc tttaatcagc taagcgatga cattgccaca
    22861 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
    22921 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
    22981 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
    23041 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
    23101 ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg
    23161 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
    23221 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
    23281 gaccgtatcc gccgccttcc gcccggaacg acctccaaag gtcttggagc gcataccgac
    23341 tccggggcgc tggaacgctg gctgcttcca gcatatcagc acgttttcgc caacgtcttt
    23401 aatggcaatc tggcgaaata cgatccctgg catgcggcac atcgtacgga agttgaagag
    23461 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggactgcg
    23521 ctctcggata tgctgcctgg tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg
    23581 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
    23641 gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta
    23701 accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat
    23761 tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca
    23821 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
    23881 gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg
    23941 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg
    24001 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt
    24061 gcgctttctt aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga
    24121 ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca
    24181 tttgtcatca gccgtcgtca ccccgacagt atcggggtga cggataatca cttactcgat
    24241 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
    24301 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
    24361 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
    24421 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
    24481 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
    24541 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
    24601 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
    24661 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
    24721 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
    24781 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa
    24841 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
    24901 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
    24961 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
    25021 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
    25081 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
    25141 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
    25201 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
    25261 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggctcag caaacagcgc
    25321 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
    25381 ctgttccact ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta
    25441 accgtccatt tcgccgtatt tcacttccag atcggccact ttatagccgt cttcttcact
    25501 catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg
    25561 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
    25621 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc agcgtcggct caaggtcgcc
    25681 gccgagaatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
    25741 gttgccgccg ccgaatttga cggaaatgtt ttcaaataac ggcttactgc cgaactgcat
    25801 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
    25861 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
    25921 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
    25981 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
    26041 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
    26101 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
    26161 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
    26221 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
    26281 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
    26341 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
    26401 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
    26461 ccaaagttga gcataagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
    26521 agtaccgcgc tgcgggcgaa ccgctaccgg ctgtcgttcc ggcaggtccg gataacccga
    26581 tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
    26641 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    26701 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    26761 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
    26821 aagcccagtt tgaaggtcag gaaatcgtgc caattaccct gacgaagagc gtgcagacag
    26881 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgatcaaa aatcgttccg
    26941 ggatgccggt tcgcctgaat taatcgtcaa aacgtaaaac aaaggcggac tcataatccg
    27001 ccttttttat ttgccagacc ttaccaggcc gggatcataa ctaacaaaac gtatcccacc
    27061 agcgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga
    27121 tcgttcgcca tacgtagcgc aatcaaattt gccagcgatc ctggtaataa accaaagccc
    27181 cccacgttta ccgcccatgc cagtaataaa gacggaggta cataattgag cagcaaaatg
    27241 gtactcggca cgttactgat cacctgcgat aaaccgactg ccgttaacca taacccgggt
    27301 tcagataaat gactcacgtt acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa
    27361 tggacgtcga taaacatcgc cataaacacc agcagcaacg tccagtccac actcaacacc
    27421 acgcgacgcg ccagcagcac aaagcccacc gccacaatca ccagtcccca cagctcttgt
    27481 ttgaactcca gcgccgtcag aaagacgata tacagcccca gacaattcca caccagccgc
    27541 ggtttccact ccggtgtttg cacccccgta tggtattgca gcgcctttcc agggaaacaa
    27601 caccagcaca acagcaggag tgtcagcatc attgcgccag ccagcggtgc catttgggca
    27661 ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca
    27721 attggcgtca gtagcgaacc agcgttgact gccagcgcct caaaaataat cagccgatta
    27781 accgggatct cacacagtct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg
    27841 acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc
    27901 cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt
    27961 aactccacac ctttggtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg
    28021 atagcagcag gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca
    28081 agaattaata actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt
    28141 tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt
    28201 gctccatgcc ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga
    28261 aattttcgac tatctgatgt ctctcgcggc tttcctgcgc cagcttctct ccttctgccg
    28321 ttaaaaacac gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa
    28381 gcattttagc caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag
    28441 cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc
    28501 gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact
    28561 gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt cctcattatg
    28621 gtgacgcata catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta
    28681 caaaaaatta acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa
    28741 tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc
    28801 aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct
    28861 atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg
    28921 cgtcgcggta cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa
    28981 aaacccactt tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg
    29041 acctgaacgg cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg
    29101 cggacccctt tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag
    29161 cacatggcgt ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac
    29221 ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa
    29281 cctcggctta ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct
    29341 tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt
    29401 ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct
    29461 ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta
    29521 cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa
    29581 agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc
    29641 gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga
    29701 tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat
    29761 tgaagagcaa gaacggatgg caaaactttc cggaatgaaa gatacttgga cggtcactgc
    29821 cgttaagccg aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc
    29881 cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt
    29941 gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct
    30001 caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg
    30061 cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc
    30121 tatcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga
    30181 agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca
    30241 agagcactcg caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg
    30301 cgacaggaca caaggcaaat acgaaacctc ctttgtgcaa tcgaaagaaa cgtcgtgcta
    30361 tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa
    30421 cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct ttaaagagcg
    30481 cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc
    30541 ttttatggtc atttccagcg gcgataaagc gcatcgggtg attaaagccc gccgctcagc
    30601 caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg aaataaatat
    30661 caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt
    30721 acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca
    30781 aaaatccgcc ccgagaggcg gattttttat atcaccaaag tgattagaag cggtaaccaa
    30841 caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg taagagaagt
    30901 ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag aaaccgtagt
    30961 cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca taacccacac
    31021 ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca gcagtgatgc
    31081 catagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc tcggtgtaag
    31141 tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat ttcaggttga
    31201 aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg ccagttacgg
    31261 tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc agtgctgaaa
    31321 gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct aagttttaaa
    31381 tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg ccatttcatg
    31441 taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt tcagagaact
    31501 gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa tcaccagact
    31561 aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt ttcaacaaat
    31621 gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc tagatatttg
    31681 tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca atagtcatat
    31741 tgctcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca ctttttcctc
    31801 tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg atcctcatcg
    31861 cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg ctgttgtttt
    31921 acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag acagtaccgc
    31981 tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg ttctcttctc
    32041 gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg ttcctgctac
    32101 cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca ctgggggccg
    32161 gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa catggccctg
    32221 cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt ggagcgcttc
    32281 aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct gtcgctattc
    32341 tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg ccaacacgga
    32401 catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg atgattttcc
    32461 tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc gccgcttcaa
    32521 tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac attaattaaa
    32581 tttacatttc tgtatggtta tgcataacca tgcagaattt ctcgctactt ttcctctaca
    32641 ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc tggcgagcag
    32701 ataaataaga attgttctta tcaatatatc taactcattg aatctttatt agttttgttt
    32761 ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga acacatagcc
    32821 ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata tgaaattatg
    32881 agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg caacgatgtc
    32941 tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaagttat ccagtttatt
    33001 gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa cttcattgcc
    33061 gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga taccatggca
    33121 gaacgtgccg tgcagctggg cggtgtagct ctggggacca ctcaagttat caacagcaaa
    33181 accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct gaaagaactg
    33241 gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga agcgaaagat
    33301 gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt cctgtggttt
    33361 atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt acccgataaa
    33421 agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca cttatttagt
    33481 gcatccatct gctatctcca gctgattaag taaatttttt gtatccacat catcacacaa
    33541 tcgttacata aagattgttt tttcatcagg ttttacgcta aataatcact gtgttgagtg
    33601 cacaatttta gcgcaccaga ttggtgcccc agaatggtgc accttcaggg tattgcccta
    33661 taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca atgagttgca aaactggcac
    33721 gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga aaaaaggaaa
    33781 tgctatgaag tctgtattaa aagtttcact ggctgcactg accctagctt ttgcggtttc
    33841 ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg ttccgtttga
    33901 atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg ccatcgctaa
    33961 agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca ttccggcact
    34021 gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg agcgtaaaaa
    34081 agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg tgaaagctaa
    34141 caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg tgaagagcgg
    34201 tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc gtcagttccc
    34261 gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg ttctgcacga
    34321 tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca aagcggtagg
    34381 tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg acgagctgcg
    34441 tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca acgaaatcta
    34501 caaaaaatgg ttcggtactg aaccgaaata ataacgctac acctgtaaag cgcactggca
    34561 gttccctctc ccccctgggg agaggattag ggtgaggggc gcaaacccgc tccggggcca
    34621 ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata tgcagtttga
    34681 ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa tgaccctgtg
    34741 gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag gttttgcacg
    34801 caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgaag tgatccgcgg
    34861 cacacctatc gtcgtccagg tgatgtttat ctatttcgcc ctgccgatgg cgtttaacga
    34921 cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact ccggcgcgta
    34981 cattgcggaa atcacgcgtg gtgcggtgct gtctatccac aaaggttttc gtgaagcagg
    35041 actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac cgctggcact
    35101 gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag acacctcgct
    35161 gtttattgtt atcggcgtgg cggaactgac ccgtcagggg caagaaatta ttgccggtaa
    35221 cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta ttaccctggt
    35281 gctgagcttt attctgcgtc gtctggaaag aaggatgaaa atcctgtgat tgaatttaaa
    35341 aacgtctcca agcactttgg cccaacccag gtgctgcaca atatcgattt gaacattgcc
    35401 cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac cctgctgcgc
    35461 tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg cctgaaggtt
    35521 aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt gttccagcag
    35581 ttttacctct tcccgcatct gacggcgctg gaaaacgtca tgtttggtcc gctacgcgtg
    35641 cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc gaaagtcggt
    35701 ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca gcagcgtgtg
    35761 gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga accgacttcc
    35821 gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tacaggatct ggctgaagaa
    35881 gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt agcttcgcgg
    35941 ctgatcttta tcgacaaagg ccggattgcg gaagatggca atccgcaggt gttgatcaag
    36001 aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaata agacacattg
    36061 cctgatgcgc tacgcttatc aggcctacag gatatctggc aacttattaa aattgcatga
    36121 acttgtagga cggataaggc gttcacgccg catccggcaa aaaagcccgc acgttgtcag
    36181 caacctgctt aatatccctt cctccctttc acccgaaagg gaggcacacc agattcctct
    36241 catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt tttctcactg
    36301 gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg
    36361 cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc
    36421 cggaaccgga tatcgaacaa aaaaaagcgg cctatggcgc actggcggat gtgctggata
    36481 acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
    36541 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
    36601 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
    36661 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
    36721 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
    36781 gcgcactgcc gctgtatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
    36841 gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac ctgctgttac
    36901 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
    36961 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
    37021 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
    37081 acgagagcgc ccgttactgg agtcgtcgcc tgagctggtt aagcagcctg attggctatg
    37141 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
    37201 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc ttccgaaata
    37261 aaaaagagat tacccagcat ttgctcaact ttgcggagca ttcgctggct tttttcagcc
    37321 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc
    37381 tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg ggtgcaacgg
    37441 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
    37501 tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag ctacaaaaac
    37561 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
    37621 cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
    37681 gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt ttcttctcgg
    37741 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
    37801 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
    37861 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
    37921 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
    37981 tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag
    38041 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
    38101 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
    38161 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
    38221 atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg gaaaacgaag
    38281 aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata
    38341 ccgcgtttac actgcgtgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
    38401 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
    38461 agacttatca ggtgctgcct gctccgggcg ctcccccggc tgaaccgctg ccgccggggg
    38521 agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg
    38581 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
    38641 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
    38701 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
    38761 taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg
    38821 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgccgcag
    38881 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttgtgga
    38941 tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
    39001 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
    39061 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
    39121 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
    39181 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
    39241 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
    39301 tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta agcgtgagga
    39361 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
    39421 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
    39481 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
    39541 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
    39601 gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcacatctc tgtcaatttt
    39661 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
    39721 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
    39781 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
    39841 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
    39901 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
    39961 cagatgttcg gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
    40021 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg caatgtaaat
    40081 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg cgtcaagtga taatgcttat
    40141 caaaattatt atcacttgcg cgaacactat cacgggatta acagtggcat cgcatccgca
    40201 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
    40261 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
    40321 tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc taacgcggat
    40381 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
    40441 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
    40501 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg
//
LOCUS       DADKXM010000018        40302 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.018, whole genome shotgun sequence.
ACCESSION   DADKXM010000018 DADKXM010000000
VERSION     DADKXM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.018"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            467..1534
                     /locus_tag="JQR05_000996"
     CDS             467..1534
                     /locus_tag="JQR05_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5323213.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1531..4266
                     /gene="rbbA"
                     /locus_tag="JQR05_000997"
     CDS             1531..4266
                     /gene="rbbA"
                     /locus_tag="JQR05_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY5323214.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4266..5390
                     /locus_tag="JQR05_000998"
     CDS             4266..5390
                     /locus_tag="JQR05_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY5323215.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5449..5724
                     /locus_tag="JQR05_000999"
     CDS             5449..5724
                     /locus_tag="JQR05_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY5323216.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5721..6080
                     /locus_tag="JQR05_001000"
     CDS             5721..6080
                     /locus_tag="JQR05_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY5323217.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6200..6601)
                     /gene="nikR"
                     /locus_tag="JQR05_001001"
     CDS             complement(6200..6601)
                     /gene="nikR"
                     /locus_tag="JQR05_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY5323218.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6607..7413)
                     /gene="nikE"
                     /locus_tag="JQR05_001002"
     CDS             complement(6607..7413)
                     /gene="nikE"
                     /locus_tag="JQR05_001002"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY5323219.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(7410..8174)
                     /gene="nikD"
                     /locus_tag="JQR05_001003"
     CDS             complement(7410..8174)
                     /gene="nikD"
                     /locus_tag="JQR05_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY5323220.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8174..9007)
                     /gene="nikC"
                     /locus_tag="JQR05_001004"
     CDS             complement(8174..9007)
                     /gene="nikC"
                     /locus_tag="JQR05_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY5323221.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYSRMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9004..9948)
                     /gene="nikB"
                     /locus_tag="JQR05_001005"
     CDS             complement(9004..9948)
                     /gene="nikB"
                     /locus_tag="JQR05_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY5323222.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPVLDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9948..11522)
                     /gene="nikA"
                     /locus_tag="JQR05_001006"
     CDS             complement(9948..11522)
                     /gene="nikA"
                     /locus_tag="JQR05_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY5323223.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11633..12220)
                     /gene="acpT"
                     /locus_tag="JQR05_001007"
     CDS             complement(11633..12220)
                     /gene="acpT"
                     /locus_tag="JQR05_001007"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY5323224.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12275..13324)
                     /locus_tag="JQR05_001008"
     CDS             complement(12275..13324)
                     /locus_tag="JQR05_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY5323225.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RNLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13423..14673
                     /locus_tag="JQR05_001009"
     CDS             13423..14673
                     /locus_tag="JQR05_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5323226.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEHVPGAASSS"
     gene            complement(14677..15234)
                     /gene="dcrB"
                     /locus_tag="JQR05_001010"
     CDS             complement(14677..15234)
                     /gene="dcrB"
                     /locus_tag="JQR05_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY5323227.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15307..15972)
                     /locus_tag="JQR05_001011"
     CDS             complement(15307..15972)
                     /locus_tag="JQR05_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY5323228.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16193..16438
                     /gene="tusA"
                     /locus_tag="JQR05_001012"
     CDS             16193..16438
                     /gene="tusA"
                     /locus_tag="JQR05_001012"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY5323229.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16540..18738)
                     /gene="zntA"
                     /locus_tag="JQR05_001013"
     CDS             complement(16540..18738)
                     /gene="zntA"
                     /locus_tag="JQR05_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY5323230.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(18812..19438)
                     /locus_tag="JQR05_001014"
     CDS             complement(18812..19438)
                     /locus_tag="JQR05_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY5323231.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRVDNAIAAACYFAGHFLIVRSLYL"
     gene            19579..19938
                     /locus_tag="JQR05_001015"
     CDS             19579..19938
                     /locus_tag="JQR05_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY5323232.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19941..20210)
                     /locus_tag="JQR05_001016"
     CDS             complement(19941..20210)
                     /locus_tag="JQR05_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY5323233.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20200..20796)
                     /gene="rsmD"
                     /locus_tag="JQR05_001017"
     CDS             complement(20200..20796)
                     /gene="rsmD"
                     /locus_tag="JQR05_001017"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY5323234.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            20946..22442
                     /gene="ftsY"
                     /locus_tag="JQR05_001018"
     CDS             20946..22442
                     /gene="ftsY"
                     /locus_tag="JQR05_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY5323235.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22445..23113
                     /gene="ftsE"
                     /locus_tag="JQR05_001019"
     CDS             22445..23113
                     /gene="ftsE"
                     /locus_tag="JQR05_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY5323236.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23106..24164
                     /gene="ftsX"
                     /locus_tag="JQR05_001020"
     CDS             23106..24164
                     /gene="ftsX"
                     /locus_tag="JQR05_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY5323237.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24409..25263
                     /gene="rpoH"
                     /locus_tag="JQR05_001021"
     CDS             24409..25263
                     /gene="rpoH"
                     /locus_tag="JQR05_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY5323238.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25534..26623
                     /locus_tag="JQR05_001022"
                     /pseudo
     CDS             25534..26623
                     /locus_tag="JQR05_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
     gene            complement(26822..27205)
                     /gene="panM"
                     /locus_tag="JQR05_001023"
     CDS             complement(26822..27205)
                     /gene="panM"
                     /locus_tag="JQR05_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY5323239.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTALQGGWEKR"
     gene            27629..28738
                     /gene="livk"
                     /locus_tag="JQR05_001024"
     CDS             27629..28738
                     /gene="livk"
                     /locus_tag="JQR05_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY5323240.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLLNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28786..29712
                     /gene="livH"
                     /locus_tag="JQR05_001025"
     CDS             28786..29712
                     /gene="livH"
                     /locus_tag="JQR05_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY5323241.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            29709..30986
                     /locus_tag="JQR05_001026"
     CDS             29709..30986
                     /locus_tag="JQR05_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY5323242.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            30983..31750
                     /gene="livG"
                     /locus_tag="JQR05_001027"
     CDS             30983..31750
                     /gene="livG"
                     /locus_tag="JQR05_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY5323243.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            31752..32465
                     /gene="livF"
                     /locus_tag="JQR05_001028"
     CDS             31752..32465
                     /gene="livF"
                     /locus_tag="JQR05_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY5323244.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            32862..34178
                     /gene="ugpB"
                     /locus_tag="JQR05_001029"
     CDS             32862..34178
                     /gene="ugpB"
                     /locus_tag="JQR05_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY5323245.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            34276..35163
                     /gene="ugpA"
                     /locus_tag="JQR05_001030"
     CDS             34276..35163
                     /gene="ugpA"
                     /locus_tag="JQR05_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY5323246.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNQVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            35160..36005
                     /gene="ugpE"
                     /locus_tag="JQR05_001031"
     CDS             35160..36005
                     /gene="ugpE"
                     /locus_tag="JQR05_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY5323247.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36085..36972
                     /gene="ugpC"
                     /locus_tag="JQR05_001032"
     CDS             36085..36972
                     /gene="ugpC"
                     /locus_tag="JQR05_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY5323248.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMNVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYFTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            36994..37737
                     /gene="ugpQ"
                     /locus_tag="JQR05_001033"
     CDS             36994..37737
                     /gene="ugpQ"
                     /locus_tag="JQR05_001033"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY5323249.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(37724..38164)
                     /locus_tag="JQR05_001034"
     CDS             complement(37724..38164)
                     /locus_tag="JQR05_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY5323250.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            38284..40026
                     /gene="ggt"
                     /locus_tag="JQR05_001035"
     CDS             38284..40026
                     /gene="ggt"
                     /locus_tag="JQR05_001035"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY5323251.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(40085..>40302)
                     /locus_tag="JQR05_001036"
     CDS             complement(40085..>40302)
                     /locus_tag="JQR05_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5323252.1"
                     /translation="NRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFG
                     FEIEGTGKKYALRNGEYVDAYYMARVK"
BASE COUNT         9313 a        10473 c        11302 g         9214 t
ORIGIN      
        1 cagggccaga gataaccact ccctggaagt cttaccagca tgaaaaaaac agaaatggac
       61 ttatgccttt gattagaagg cgcacaatgt tacagctctg aaccatagac aacatgaata
      121 ttaatgttac tatctggttg agactgttga taaaacgtaa aaaggaatgc tctgttcctg
      181 caactgaaca ggcgtgaaac gcgatccaac aggatggata ccgttttgtc agccagtttt
      241 atagatcaca cttatgagta tgcgtgtttc atttacggcc aggaatgctg atgatgacaa
      301 cattactacc cgttttcact aagccttccc cgttggcgct caatgctctg cgcgctggtc
      361 gtatttgtcg tttccttctt atcccggatg gaagaatccg ctgaacgtcg ttcggcggca
      421 gttgtaacac gcgtcgtttg cttacaggga ttatggcggg tcgattatgg ataagagtaa
      481 gcgccatctg gcgtggtggg ttgtcggggc actggcggtg gctgccgtcg tggcgtggtg
      541 gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc agcaatgggc gcattgaagc
      601 gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc gacaccattc tggtgaaaga
      661 aggccagttt gttcgcgaag gcgaagtgct ggcgaagatg gatactcgcg tgttgcagga
      721 acagcgactg gaagccatcg cgcaaatcaa agaggcacaa agcgccgttg ccgccgcgca
      781 ggctttgctg gagcaacgac aaagtgaaac tcgtgccgca cagtcgctgg tcaatcaacg
      841 ccaggccgaa ctggactccg ttgccaaacg tcatacgcgt tcccgctcgc tcgcccaacg
      901 aggggctatt tctgcgcaac agctggatga cgaccgcgcc gccgctgaga gcgcccgagc
      961 tgcgctggaa tcagcgaaag cccaggtttc ggcttctaaa gcggctatag aagcagcacg
     1021 caccaatatc attcaggcgc aaactcgggt cgaagcagcg caagccactg aacggcgcat
     1081 tgccgcagat atcgatgaca gtgaactgaa agccccgcgt gacggacgcg tgcagtatcg
     1141 ggttgccgag ccaggggaag tgctggcggc aggcggtcgg gtgctgaata tggtcgatct
     1201 cagcgacgtc tatatgacct tcttcctgcc aaccgaacag gcgggcacgc tgaaactggg
     1261 cggtgaagcc cggctgatcc tcgatgccgc gccagatctg cgtattcctg caaccatcag
     1321 ttttgtcgcc agtgtcgccc agttcacgcc aaaaaccgtc gaaaccagcg atgaacggct
     1381 gaaactgatg ttccgcgtca aagcgcgtat cccaccggaa ttactccagc agcatctgga
     1441 atatgtcaaa accggtttac cgggcgtagc gtgggtgcgg gtgaatgaag aacttccgtg
     1501 gcctgacgac ctcgtggtga ggttgccgca atgacgcatc tggaactggt tcccgtcccg
     1561 cctgtcgcgc aactggcggg cgtgagccag cattatggaa aaaccgttgc gctgaacaat
     1621 atcactctcg atattccggc ccgttgcatg gtcgggctga ttggtccgga tggcgtcggg
     1681 aagtcgagtt tattgtcact gatttccggt gcccgcgtca ttgagcaggg caatgtggtg
     1741 gtgctgggtg gcgatatgca cgacccgaag catcgccgag acgtctgccc gcgcatcgcc
     1801 tggatgccgc aggggctggg caaaaacctc taccacacct tgtcggtgta tgaaaatgtc
     1861 gattttttcg ctcgcctgtt cggtcacgat aaagcggagc gggaagtgcg aattaatgag
     1921 ctgctgacca gcaccgggtt agcaccgttt cgcgatcgtc cggcaggtaa actctccggc
     1981 gggatgaagc aaaaacttgg gctgtgctgc gcgttaatcc acgacccgga actgttgatc
     2041 cttgatgagc caacaacggg ggttgacccg ctctcccgcg cccagttctg ggatctgatc
     2101 gacagtattc gccagcggca gagcaatatg agcgtgctgg tcgccaccgc ctatatggaa
     2161 gaggccgaac gcttcgactg gctggtagcg atgaatgccg gagaagtgct ggcaaccggc
     2221 agcgccgaag agctgcggca gcaaacgcaa agcgccacgc tggaagaagc atttattaat
     2281 ctgttaccgc aagcgcaacg ccaggcgcat caggcggtag tgatcccacc gtatcaacct
     2341 gaaaacgcag agattgccat cgaagcgcgc gatctgacca tgcgttttgg ttccttcgtt
     2401 gccgttgatc acgtcaattt ccgcattcca cgcggggaga tttttggatt ccttggttcg
     2461 aacggctgcg gtaaatccac caccatgaaa atgcttaccg gactgctgcc cgccagcgaa
     2521 ggtgaggcgt ggctatttgg gcaaccggtt gatccaaaag atatcgatac ccgccgtcgt
     2581 gtgggctata tgtcgcaggc gttttcgctc tataacgaac tcaccgtgcg gcaaaacctg
     2641 gagttacatg cccgtttgtt tcacatcccg gaagcggaaa ttcccgcgcg agtggctgaa
     2701 atgagcgagc gttttaagct caacgacgtt gaagatgttc tgccggagtc attgccgctc
     2761 ggcattcgcc agcggctttc gctggcggtg gcggtgattc atcgcccgga gatgttaatc
     2821 ctcgatgagc ctacttctgg tgtcgatccg gtggcgaggg atatgttctg gcagttgatg
     2881 gtcgatctct cgcgccagga caaagtgact atcttcatct ccacccactt tatgaacgaa
     2941 gcggaacgtt gcgaccgcat ctcactgatg cacgccggaa aagtgcttgc cagcggtaca
     3001 ccgcaggaac tggttgagaa acgcggagcc gccagtctgg aagaggcatt tatcgcctat
     3061 ttgcaggaag cggcagggca gagcaacgaa gccgaagcgc cgcccgtggt acacgacacc
     3121 acccacgcgc cgcgtcaggg atttagcctg cgccgtctgt ttagctacag ccgccgcgaa
     3181 gcgctggaac tgcgacgcga tccagtacgt tcgacgctgg cgctgatggg aacggtgatc
     3241 ctgatgctga taatgggtta cggcatcagt atggatgtgg aaaacctgcg ctttgcggtg
     3301 ctcgaccgcg accagaccgt cagtagccag gcgtggacac tcaacctctc cggttcccgt
     3361 tactttatcg aacagccgcc gctcaccagt tatgacgagc ttgatcgtcg gatgcgtgcg
     3421 ggcgatatca cggtggccat tgagatcccg cccaatttcg ggcgcgatat cgcgcgtggt
     3481 acgcctgtgg aactcggcgt ctggatcgac ggagcgatgc ccagtcgtgc cgaaacggta
     3541 aaaggttacg tgcaggcaat gcaccagagt tggcttcagg atgtggcgag ccgacaatcg
     3601 acacccgcca gccaaagcgg gctgatgaac attgagacgc gttatcgcta taatccggac
     3661 gtaaaaagcc tgccagcgat tgttccggcg gtgatcccgc ttctgctgat gatgatcccg
     3721 tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagc tagggtcgat tatcaacctt
     3781 tacgtgaccc ccaccacccg tagcgaattt ttgcttggca aacagctgcc gtacatcgcg
     3841 ctggggatgc tgaacttttt cctgctctgc ggcctgtcgg tgtttgtgtt tggcgtaccg
     3901 cataaaggca gtttcctgac gctcaccctg gcggcgctgc tgtatatcat cattgccact
     3961 ggaatggggc tgctgatctc cacctttatg aaaagccaga ttgccgccat tttcggaacg
     4021 gcgattatca cattgatccc ggcgacacag ttttccggga tgatcgatcc ggtagcttcg
     4081 ctggaagggc ctggacgttg gatcggcgag gtttacccga ccagtcattt tctgactatc
     4141 gcccgcggaa cgttctcgaa agcgctggat ctgactgatt tgtggcaact ttttatcccg
     4201 ttgctgatag ccatcccgct ggtgatgggc ttaagcatcc tgctgctgaa aaaacaggag
     4261 ggatgatgcg ccatttacgc aatattttta atctgggtat caaagagttg cgcagtctgc
     4321 tcggtgataa agcgatgctg acgctgattg tcttctcgtt tacggtgtcg gtgtattcgt
     4381 cagcgaccgt tacgccagga tcgttgaacc tcgcgccgat cgccattgcc gatatggatc
     4441 aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg tccgtggttt ttgccaccgg
     4501 agatgatcac cgccgatgag atggatgccg gactggacgc cggacgctat accttcgcga
     4561 taaatattcc gcctaatttt cagcgtgatg tcctcgccgg acgccagccg gatattcagg
     4621 tgaacgtcga tgccacgcgc atgagccagg catttaccgg caatgggtat atccagaata
     4681 ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg tgataacagc gaaccgttgg
     4741 tatcgctgga aacccggatg cgctttaacc cgaaccttga tcccgcgtgg tttggcgggg
     4801 tgatggcgat catcaacaac attaccatgc tggcgattgt attgaccgga tcggcgctga
     4861 tccgcgagcg tgaacacggc acggtggaac atttactggt gatgccgata acgccgtttg
     4921 agatcatgat ggcgaagatc tggtcgatgg ggctggtggt gctggtggta tcgggattat
     4981 cgctggtgct gatggtgaaa ggtgtactgg gcgtaccgat tgaaggctcg atcccgctgt
     5041 ttatgctggg cgtggcgctc agtctgtttg ccaccacgtc aatcggcatt tttatgggga
     5101 cgatagcgcg ttcaatgccg caactggggc tgctggtgat tctggtgctg ctgccgctgc
     5161 aaatgctttc cggtggatcc acgccgcgcg aaagtatgcc gcagatggtg caggacatta
     5221 tgctgaccat gccgacgaca cactttgtta gcctcgcgca ggccatcctc taccggggtg
     5281 ccggattcga aatcgtctgg ccgcagttcc tgacgctgat ggcaattggc ggcgcgtttt
     5341 tcaccatcgc actgctgcga ttcaggaaga cgattgggac aatggcgtaa ggaagggaaa
     5401 cttgacttca tactgtgatg tcataaagtg aagtcataag gacatgccat gagaaccaag
     5461 gtacaggctt tacggaagaa acaaaaaaat acattggatc aaatatttaa aactcctgtt
     5521 cctcaaggga tcaagtggtc tgatatagag tcactggtta aagcattagg cggagaaatt
     5581 aaggaaggaa gaggttcgcg ttgcaagttc atactaaata tgagcgttgc gtgtttccat
     5641 cggcctcatc cgtcgccaga taccgataaa ggcgctgtag aaagcgtgcg tgactggtta
     5701 ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc aaactctatg gagattgccg
     5761 ggcaaccagc tgttattacc tacgtgccag aactgaatgc atttcgtggc aaattcctgg
     5821 gcttgtcagg ttattgtgat tttgtctctg acagtattca ggggctgcaa aaagaaggag
     5881 agctttccct gcgagagtat cttgaggatt gtaaagctgc gggtattgag ccgtatgcgc
     5941 gtacagaaaa gattaagacc tttacgttac gctatccaga atcgctgagt gaacgcctta
     6001 ataatgcggc tgcgcagcaa caagtctctg ttaataccta tattattgaa acactgaatg
     6061 agcgtttgaa tcatttataa attttagcga cgtacattac gcagaatatc tgcctttaca
     6121 ggatctatct atgcaactga acttaatttt ataaattaag ttcagttaat atcttttctt
     6181 tccttgtttt agaaataact caatcttcct tcggcaggca ctgcaaatgc ccgtgccgca
     6241 cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac gtcacccatg tcacctttca
     6301 acacggcgat ttccagacag tcgtcgtggt tgatgtgcac atgtagcgtg gcgacggaga
     6361 ggtcgtggtg atgatgctgg gtggaaacaa tacggctggc taagtcgcgt ttttcgtgtt
     6421 catacacata cgacagcacc gcgaaacctt gtgtgccgtg ctgctgggta gcttcctgcg
     6481 ccagggcact acgcagaata tcgcggatag cttcggaacg gttgttataa ccacgacgct
     6541 ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag cgtgatggtg actcgttgca
     6601 ttggcgttaa accttttctg aggtgcgacg gcgcacgggg aatgcgggta ataccgcgtt
     6661 ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta tctcccaccg cctgggtttc
     6721 gacgatttgt ccgttgtcca ttaccatcac tcgctggcaa aagcgttcca ccaggcgtaa
     6781 gtcatgagtg atgaacaggc aggcagtgcc aaactgttgt tgtagctttt tcagcaggcg
     6841 aatgacgccc gcctgtaaca cgagatcaag gttagaaacg gcttcatcca gaatcagcaa
     6901 tttcggttcg accgccagcg cgcgtgccag gcagacgagc tggagctggc cgccgcttaa
     6961 ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg accgccttca gcatttcgct
     7021 ggcgcgcgcc agttgttcgg attttttcag tgatagcagg tggcgcatcg gttcacgcag
     7081 gatctcgcgc acggttttgc gcggattcac ggcactgatg gagtcctgaa acaccatctg
     7141 aatatcacgg cggaaggctt tacgttgggc gcgattaagt ttcgccagcg attcgccgcg
     7201 ccagctaata ttcccttgtg agggagactc taagcctacc agcagccgcg cgagggtgct
     7261 ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg ccgcttttca gggtcaggga
     7321 aacgttattc agcaccgcct gatgttgatg ttttccgcta aatccaccgt gggcatagtg
     7381 atgggaaagg ccgcagacgt taagtaaggt catgatgcca gctccatacc gtagagggcg
     7441 agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg gggcgttaaa cagcgtttct
     7501 acatcgccct gttcgacaat tttaccttgt gacatcaccg ccacgtcatc cgccagacgc
     7561 gccacaacgc ccatatcatg ggtgaccagc agcattcccg gcgcttgttt ttgcataatg
     7621 ctttccagca gatcgaggat gcgcgcctgt gctaccacgt cgaggtcggt ggtcggttca
     7681 tcggcgatga taaacggtga ttcacacagc accgccatcg caatcatcat gcgctgcaac
     7741 atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc gcgcggcgtt ttccagcccc
     7801 accgcttcta tggcagcggt aagcgtagcg tcatcggcgg gtttccctaa cgccaggcag
     7861 gtttcccgcg cgtgggtgtg catggtgtgc agtggattaa aggcgctgcg cgggttctgc
     7921 atgatggtgg caattttgat gccgcgcagg gcgcaaggcg aaaccggttt gccatcggct
     7981 aaaatttccc ccgctgtctg gcgaacgcca gcgggcaaaa tgcccagcgt cgcggcgcag
     8041 gttaatgatt tcccgctgcc gctaccgccg actaacgcca gcacgcgccc gcgttgcagg
     8101 gttaacgata caccgtgtac cagcggctgc gcggcctgta gcgcgatatt acgtagttca
     8161 atctgttgcg gcattagtgt gcgtgctccg tcaccagatg aggatccaga tgatcgcgca
     8221 gtgcgtcacc caccaggtta aaggccatca cgctgataaa cagcgccagc cccggccaga
     8281 acatttgcag cggctgggtc cagatatact ggcgcgcgtc gttaatcatc acgccccatt
     8341 cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat ccccgcgacg tgcagcatca
     8401 tatggccgat atccagcgtt gccagcacca gcagcgaagg gatcaccgcg cctgccagat
     8461 gatcgacaaa cacccgcaca tggcccgcac cggaaagccg tgacgccagc acaaactcgc
     8521 gttggcgtag tgaaatcacc aagctgcgca ccatgcgtga ataccacgcc cagtgcgaca
     8581 gggcgatggc gataattacg ttggtcagcc cggtgccgag cacgccaacc ataaagaacg
     8641 acagaatcga ggtcgggaag gtcataaaca tatcggcgac gcgcatggtg gcctgatcaa
     8701 cgcgcccgcc aatcaacccg gcgctgccgc caataaccag ccctaatgtc agcaccagca
     8761 gcaggcaggc cattaccgaa ccgagcgaca tgcgggtcgc tgccatcagc cgcgagaaaa
     8821 tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc atccggcgaa agcaggcacg
     8881 acggcaaatc aattgcctgt ggatcgtacg gcagccacca ctggctggtg agcgcaatca
     8941 gcgccagcag ggcgataatg atcagcgcca ggcgtaccga ccagcgggaa gagaggaaaa
     9001 agttcacgcg tgcgctcctt catgacgacg aatgcgcggg tccagcgcgg cgttgagcaa
     9061 atcgacaatc aaattacaga ccacaaaaac caccaccatc atcagcgtaa agcactgaat
     9121 caccggatag tcacggttaa aaatcgccga caccgcatag cgcccgacgc caggccaggc
     9181 aaagatgttt tcgataatca tcgtcccgcc aatcaattcg ccaatgtgca tccccacggc
     9241 ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg cgttcggtct gtttgtcatt
     9301 caggccgcgt agccgcgccc aggtgacgtg acgctgaccg gcgacgtcca gcatactggc
     9361 gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg atggaaaccg caggtaaaat
     9421 gatgtgctgc cagccgccgt agcccatcgc gggtagccat tgcagataca ccgaaaacgc
     9481 catcactagc aaaaatgcca gccagaagtt gggcatcgac acgccaagaa acgcgataaa
     9541 gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc gcccagatac cgagcggtac
     9601 ggaagtgagc agaattaata ccagcgccgc acctgcaagt tccagcgtgg cgggcaggaa
     9661 gttcagcata tcgtccagta ccgggcgttg gctggcgaat gagataccaa agtcaagatg
     9721 cagcgccttc cacaaccagg taccgtactg gacgtacagc ggctgatcca gcccgagcat
     9781 ggtgcgggta gaggccagca tctccggcgt cggcggcagg ttagataaac gcagataatc
     9841 gagcaccggg tcgccggtac cgaggcgcag catcagaaaa atgatcaccg aggcggcgag
     9901 caccatcggg atcagcagca gaaagcggcg taatacgtaa cgcaacatta aggtttcacc
     9961 ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg cgtaggggat attacccagc
    10021 tccggttttg ataccaccat catcgagatg tagctgatag gcagataaac cgcctcgtca
    10081 tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt gcgtttcgtc atgggtcgcc
    10141 agcacttcgc cgatctcttt atcaatcagc ggcttgtcgg ctaatccttg ctgtgcctgg
    10201 aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg cgtgtggatc gtatggcgcg
    10261 ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc gctgacgagc atagatactg
    10321 ctctcttctt cgccaatcag cgagacatct gcgccaatct ggcgcatatc agcctgaatg
    10381 atttccgcca tcgatttgct taacgcatcg gtgccgatga acgacagttc aatgcgcagc
    10441 ggctgaccat ttttctcgcg gatgtctttg cccgcaggca gcgtccaacc ggctttttcc
    10501 agcaactctt tcgctttctg cggatcgtac tggcgcggtt tcaggccgag attagcgtaa
    10561 ggcacagaag gggcaaacag ggtgtcggcg acctgctggg tgccatataa cgcgttatca
    10621 atcagcgatt ttttgtttac cgcgtaatta agagcttcac gtactgccag ctcgttggtg
    10681 ggggctttgg cggtattgag cgccagcatc acggtttcga tcggctgtga cagttgggtg
    10741 tggtaagccg gattctggct aaagcgggcg aaggtatcga gcggtaataa cccttcgttt
    10801 ccgtacagca ggtcgatatc gccagtttca aacgccaccg cgcgggtagt cgggtccggg
    10861 atgacgttaa aggtgatctt tttaatcgct ggcttttcgc cccagtagtt ttcgttacgg
    10921 acgaagacat cgtactgatt cagtttcgat tcctgcaaaa tccacggtcc ggtgccaatc
    10981 ggcgctttaa ttccgttcat ggtttcatgg tttttaaact gcgagggagc gataaaacgg
    11041 aaaggacggg gcagggccag ttcttgcagg aaaggatagt aggcgctttt cagggtaatt
    11101 tgcagctctg ttttactgag tgctttaaca tcaacaatct ggtttgccag ctccagccag
    11161 gcgtgacgtt gacggttatc gagcactgcg cggaagtttt ctgccgccgc ctcggcatcg
    11221 aacggttcac cgttggagaa tttcacgtca tcacgcaggg tgaaggtcca ggttttacca
    11281 tcttctgaat gagtccagct ttttgccagc cacgggatca ccgaaccgtc tgcctgatat
    11341 ttcaccaatg gttcataaac catgctctgg gcgaacatct ggttaggcgt gtaaaggtgc
    11401 gggtttagtg gcccgacatt caccggccag gcggtggtga tttcatctgg tgcagcggca
    11461 tggacgataa aagacgcaca agccagcagc gcaaatagag tgcggcggag tgtggagagc
    11521 atggtaaccc caatggatta aaatagatgg cggaaataag tatgacgatt ttaagtattc
    11581 gtcatactga ttacctgttc tggatcaata gatgggcagt cgggtgggcg gatcagttaa
    11641 ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa gggggtgggg gtgcagatcg
    11701 caaggcttaa attttctagc tgacaatggc tgactgacag cgaggagtga taggtgctat
    11761 ctacgctgac gatttgccag gcgctgccgc cacgctgttt aacgatggct tctttgcgcg
    11821 tccagatgcg ccagaacatt tccagttgct gatcaggatg cacggcgtcc atttcagcgt
    11881 gttccccgag gctgaacacg gcgttcgcca gccagcgcca gttggcgcgc gggcgaatca
    11941 cttcgatatc gcagccgact tcgccttcat cgctcaacag cagagcgata tcgtcaccgc
    12001 tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa tgcaggtttg ccttgttcgc
    12061 catagatgat ctccggtagc ggggaaagcg tgtgcgaaag caatgcacgc cccgccagcc
    12121 agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa acccggtggc agtggagctg
    12181 cgcttaaggt cgaaactttc cccagaacta tccgatacat atcagggcca acgtttaatg
    12241 gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc cggtaaccga cttttcggtc
    12301 tgcccggccc cagtaaaatc gccagtttgc tgccaccttt cgtcgtttcc atccagattt
    12361 tacacacgct ggttaatggc accgaaagta gcatccctac cgggccgagc agccatcccc
    12421 agattaacaa tgaaagaaat accaccatgg tggacatccc cagtcgatgg cccatcatcc
    12481 gtggttctaa aatattgccg atgaccatat ggaccactaa aaacaatgcg ccgaccagaa
    12541 tacattcgta aacaccatta aacagcagca cctgaatcat tggcggtacg gcggaaatta
    12601 ccgcgccgat attgggcacg tagttgagca aaaacgccag tactgcccac atcagcgcaa
    12661 actgtacgcc cattagcgcc agccccagcc agacgatgac acctgtccat aaactgagta
    12721 gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc gcggtgtaat cctgcgatgt
    12781 gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg gacgtggcgc acttcaaaca
    12841 gcataaaaac tacggtcatc accagcaaaa gcacgctcgc cattgccccg gaaagcccgg
    12901 tcattagcgc tgtggtgaag gtaaccactt tttccgagtc catccgctgc agcattcgct
    12961 ccggcgacat atgcaaatta agaaaaggca acatctcctg caatttaaaa aggttgcgcg
    13021 tcagctcctt attaaacttc ggcagcatag agataaattc gttaaacgat gccgccagta
    13081 cgccgaccag cgcggttagt gcgatcagca tcaccaccac tacaatcgta atggcaacgg
    13141 ggcgttgtac tccccgacga ataaaccagg taacgagcgg gttgaggacg atggcaaaaa
    13201 acagcgccag taacagctgc acaatgatat ctgccgctgc gtgaatgccc gcgaggatca
    13261 ctaccagcga ggccaacttg agcagaatgt gcatgcccgt tttatcgggt tgaggggttt
    13321 ccattggggc ttccttgtga ctttttgtgt taagtgtagc gggagtcacg ccagcaatat
    13381 tctgattctt actgctgatt ttcacgtcag cacatggtaa aaatgaaaca ctgttgtaaa
    13441 aatgtggtga tcctcatgcc cgaacccgta gccgaacccg cgctaaacgg attgcgcctg
    13501 aatttgcgca ttgtctccat tgtcatgttt aacttcgcca gctacctcac catcgggttg
    13561 ccgctcgctg tattaccggg ctatgtccat gatgtgatgg gctttagcgc cttctgggca
    13621 ggattggtta tcagcctgca atatttcgcc accttgctga gccgccctca tgccggacgt
    13681 tacgccgatt tgctgggacc caaaaagatt gtcgtcttcg gtttatgcgg ctgctttttg
    13741 agcggtctgg ggtatctgac ggcaggatta accgccagtc tgcctgtcat cagcctgtta
    13801 ttactttgcc tggggcgcgt catccttggg attgggcaaa gttttgccgg aacgggatcg
    13861 accctatggg gcgttggagt ggttggctcg ctgcatatcg ggcgggtgat ttcgtggaac
    13921 ggcattgtca cttacggggc gatggcgatg ggtgcgccgt taggcgtcgt gttttatcac
    13981 tggggcggct tgcaggcgtt agcgttaatc attatgggcg tggcgctggt ggccattttg
    14041 ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca aaccgctgcc gtttcgcgcg
    14101 gtgcttgggc gcgtctggct gtacggtatg gcgctggcac tggcttccgc cggatttggc
    14161 gtcatcgcca cctttatcac gctgttttat gacgctaaag gttgggacgg tgcggctttc
    14221 gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt tgttattccc taacggcatt
    14281 aaccgtatcg gtggcttaaa cgtagcgatg atttgcttta gcgttgagat aatcggcctg
    14341 ctactggttg gcgtggcgac tatgccgtgg atggcgaaaa tcggcgtctt actggcgggg
    14401 gccgggtttt cgctggtgtt cccggcattg ggtgtagtgg cggtaaaagc ggttccgcag
    14461 caaaatcagg gggcggcgct ggcaacttac accgtattta tggatttatc gcttggcgtg
    14521 actggaccac tggctgggct ggtgatgagc tgggcgggcg taccggtgat ttatctggcg
    14581 gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc gattaaaaaa acggcctccg
    14641 gaacacgtcc ctggggccgc ctcatcatct taaaacttac tgaataacca gcgtattaat
    14701 gatgttttct gcggtggttt gcgctttttg ctgatcgtca gctggcagcg taatttgcat
    14761 ggtcagcagt tgattaccca cgttacccag aataacggaa gagtacgccg tctggccttt
    14821 cgcggagata atactgtcta actgctgcat tttgtgacct ttcagctcaa tggctttatt
    14881 ggttaccact tgcagctgcg gatcgcggct acgttgctga tcttccagac gcttcgccaa
    14941 caccgccaga tcttctttcg gatcatcgcc catgatgacg atgactgctt tctgcccggt
    15001 ggcgtcggac cagacatgca tgttattggc ctgcgttccc agcttaccgc tctggtcggt
    15061 catatccgct ggcagcgaga aacttaactt gccatcaagc aggttgacgg gattcccggt
    15121 agcgttactt tccgcgaccg aaccctgcgc cgtagcgtta gtgtctttat catcacaggc
    15181 cgcaagcccc ataaccagca ggccaattcc gacatattta accagattgc gcattgactt
    15241 cttcctttcg ataaacggcc ataacggctc attcatccat cttatcacaa ctctgataac
    15301 gaacctttaa ctcgcctgca aagcgttgat ttcggattta tctgccagtc ttttcaacag
    15361 catattgagt aatacgccat acattggcag gaagaaaacg atactgatta acactttgaa
    15421 acagtaatcg accagcgcga tttccatcca gtgttcagcc ataaaggcat ccgggctacg
    15481 ccagaaggca atgaagaaaa aggccagcgt gtcgctgacg ttaccgaaca gtgtggacgc
    15541 tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg ttaaaaacgt gcacgtcgag
    15601 gatttgcccc agcgcgtagg ccatgaaact ggcggtggcg atacgggcga caaacaggtt
    15661 gaagtgggcg agtgcgccga atccctgcca ggaacccata tagaatagcg acgagatgac
    15721 gtaggagatt aataacgcag ggatcattac cgcgaagata atgcgtcggg ccagcggtgc
    15781 gccaaaaata cgcacggtca ggtcggtagc aagaaaaata aacggaaagc taaacgcgcc
    15841 ccaggtggta tggaaaccaa aaatggagac gggaagctga accagatagt tactggaggt
    15901 gatcaccaga agatgaaata acgataacca gaacaacgcc ttatagcgtt gagtttgcga
    15961 gaaaacgttc atattgtacc tttttgatta accattgggg tgagggaacc caatacgtac
    16021 gacacgtctg ttaccttaac ataataacgt gcttaccctt ttttcgagcc gccgcatgat
    16081 actgctttac gttgacaatg caatggttaa ttttcacgca atcgttaacc tagtttgctt
    16141 acggactcac agggcgtaaa ctagcgccgt ttttttaagt gatgagaaga aaatgaccga
    16201 tctcttttcc agccctgacc acacactcga cgcgcttggc ctgcgctgcc cggaaccggt
    16261 gatgatggtg cgcaaaaccg tgcgcaatat gcagcctggc gaaacgttgc tgattatcgc
    16321 cgacgatccg gccactaccc gcgatattcc tgggttttgt acctttatgg aacacgaact
    16381 ggttgcgaaa gagacggatg gactgcctta tcgttatttg attcgtaaag gcggttgata
    16441 ggggctgatt ggcttcgatg ccgccttttc ctcaccccct aaccctctcc ccagaggggc
    16501 gaggggaccg atcgcgctca atgttgcgat cggtttgcct tatctcctgc gcaacaacct
    16561 taacgcattc gccgtcacca gcaccgtcgc acccgtatct gccagcactg ccagccacaa
    16621 cccggtcatc cctaaaagcg tggtgacgag gaagatccct ttcagtccca gcgcaatggt
    16681 gatgttctgg cggatattgg cgtgagtggc gcgtgccagt tcaatcattt gcaccaggcc
    16741 gcgcaggtgg ttatgggtta atgctgcgtc ggcggtttcc agcgccacgt ctgtgccgct
    16801 acccatagca atcccgatgg cggcagcttt catcgctggc gcgtcgttaa taccatcacc
    16861 gaccatcgcc aggggcgcat gttgattcag cttggtcacc gctttgactt tatcttccgg
    16921 caataggccc gctttaaact ccagccccag ctccccggca attgccgccg ctgcgcgtgg
    16981 attatcgccg gtgaggatca ctcctttaac ccccagcgcg ttcagttcac tgatggcagt
    17041 tgcagcgtcg gcgcgcaggg tatcctgcaa tgcaatgata cccagcacgt catcattacg
    17101 tactaccagc acaaccgttt gcccggcgct ttccagttca ttaatcaaac cagcaaatgc
    17161 atcagcggga tgtttcccgg cagcgcatat caacacgcgc tcaccattaa cctgcgcttc
    17221 aatgccagac ccgaccagtg cccgctgtga ttcggcggtg ggaatggcga gttcagcaac
    17281 ctgtgcttcg cgtacgatgg cttgcgccag tggatgcgtc gcgccttgct cgaccgccgc
    17341 cgccagtgtc agcagttcag attcactaat acccgttgcc ggatgaatcg cggtaacgcg
    17401 cggtttaccg acggtcagcg taccggtttt atcaaacgcc acttgggtaa cacgacctag
    17461 ttgttccagc gccgcaccgc ctttaattaa cgccccacga cgcgctgctg ctgccagccc
    17521 ggaggttatc gccgcaggcg ttgagataac taacgcacac gggcaaccaa tcagcagcag
    17581 cgtcagacct ttataaatcc actcctgcca gctggcggca aacagcagcg gcggcactag
    17641 cgttaccagc agggcgacgg ccataatcgc tggcgtataa atacggctga aacggtcgat
    17701 aaaccgctca atgggggcgc ggcgctcttc ggcttcttcg atcagtttca gaatccggtc
    17761 aatggcgctg gcacccggtt ctgacagcac ttccagcgtt accagacggt ctacgctggt
    17821 ggctcctgca ggaactttat cgcccgtcgc gcgctccacc ggaatggatt ctccggtcag
    17881 ggcgctttca tcaaaactgg caaacggtga gagcagttta ccgtcggcag gcaaacgccc
    17941 acctgcggcg acttcaatca catcgccagg ccgcaggctg ttaatcgcca cctcttcccg
    18001 ctcaccgtta cgcaggcgcg tggcggtttc tggtttcagc gccattaacg cgctaacgcc
    18061 ctgacgcgcg cggctggcgg cccagccttc cagtcgttca ccaatcaaaa acagcagcaa
    18121 caccatcgca gcttcagccg ttgcgccaat aaacagtgca ccaatagcgg ctacgctcat
    18181 taaggtttca atggcgaagt agctgccgga tttgatcagc cgtaatgcct gacgagcaat
    18241 cgggtacagc ccaactagcg tggtcgcgat aaacgccagt tgcccgaacg gatgattgaa
    18301 ctgctccaga ccccagctga ttgccatcat cacgataagc gtaatcagcg gcagattctc
    18361 tttcaggcgc gatgcttgtg gctcgtcggc ggcctgctca tcgcgcaggg aatagcccgc
    18421 tttttgcacc gcagattcaa cctgggcacg gatgtcattg tcggcatcaa ccaccagttt
    18481 ttcggtggcg aacaacacct gcacctgatt cacgcctgca agctggcgca cggcattttc
    18541 taccttgcgc gcacaggcgg cgcagtccat gccgctgact ttccagctat agcgggtgcc
    18601 ggagacgttt tcagagagcg ttggcgagct ggagcatgcg ccgtcgcagc aacagtcgtt
    18661 ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat tgaggggctt tcttgccgtg
    18721 attgtcaggt gtcgacatgg catcctccgg ttaagttttt tctcattaac cgaaggatac
    18781 actctggagt cgactccaga gtcaagtttt atcagagata cagtgagcgg acgatgagga
    18841 agtgaccggc aaagtagcag gccgcagcaa tcgcgttatc cacacggaag cgtcggcgat
    18901 agtggctccc cagccagaca aagttactga taaacagcaa cgaagcgcca acaaacgcag
    18961 agagcgccgg agcggtcgga cggaagaacc acagttcacc tgccagccac accatcacca
    19021 gcgtcatgcc gataaacgtg cagataggcc aacggtactc ttccagccgc gtccagataa
    19081 tcgccagtag cagcgcaccc agaaccagca acaccagtgg cagaggccag aagaaagaga
    19141 gcgtcatctg actggcgaaa tagatggtgt acagcaggtg cgagaggaaa aacgcgccga
    19201 tggcgtacat cagacgttga cgtggcaaca gggttagcgc atcgcccagc agtgaggcgc
    19261 acagtcccgc cagcaccaga tagctaatgg cgtcgaacat cggtgcttgc caggccagta
    19321 acagcaggag aagaagggtt aacggtttaa acacccagcg ttgccaggtt ggcccacgat
    19381 acgatgcatc cacagatagc catgcggaaa gacagacagc gataaacgac caaagcatct
    19441 tagttccttg aattcgttat tttttcgtaa gcggaatgcc tacggtcttg cttccagttt
    19501 agtggcgagg gtggacggat gacaacacca taactggcag ggtatgctta attccgcatt
    19561 ttccgatgag ggataaagat gagtaaacca cctcttttct ttattgttat cattggctta
    19621 attgtcgtcg ccgcatcgtt tcgttttatg caacagcgac gggaaaaagc tgataatgat
    19681 atggctccgc tccagcaaaa gctggtggtg gtgagcaaca agcgggaaaa accgattaac
    19741 gatcgccgtt cgcgccagca ggaagtgact ccggcaggca ccagtatgcg ctatgaggca
    19801 agcttcaaac cgcaaagcgg aggaatggag cagacgtttc gcctcgacgc ccagcagtac
    19861 cacgccctga cagtgggcga taaaggtacg ctgagctata aaggaacgcg ctttgttagc
    19921 tttgttggcg aacaataagg ttatttttta actttaaatt tcttctgcca gaccagcagt
    19981 tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg tggtcatctg cgggccatct
    20041 ttcggtaaag tggatttcaa tagcgccagc tgcataccat gcatcagcac ggtaaaaatc
    20101 agcgccacgt taacgaaaat atttagcggg cgcgggaagg gatgcaccag attgaggatt
    20161 aaaaatcccc aaacgcagag cattaacaaa cgaccaatat taatcagcat cactttctcc
    20221 ttgtgcttcg tgttgataca gccgataagc cacctgaccc gccacttttt cccgatgtaa
    20281 tgaccagttt gccggaacag tgggcagacc gttttcgact tcgctttcga cataaatcag
    20341 ggcttcgtca gccagccagc cgttatcttc cagtaaattt atcgtctctt ctaacaagcc
    20401 acggcggaac ggtggatcga caaacacgat attatgcggt gtgccttttt gcgccaggaa
    20461 tgacattgcg ttgctgttca ccacgcgtgc attgcctgct tttagtgtcg ccagattctt
    20521 aattaactgc tgagaaacca cgcgatccat ctcaatcaac gttgcccccg cagcgtagcg
    20581 cgataacgct tccagcccca gcgcgccgct cccggcgaag caatccagac attgggcgtc
    20641 aacaatgacc ggagccagcc agttaaacaa cgtttcgcgt acgcggtcgg tggtggggcg
    20701 cagacctggg ctatcaggaa ccgggagttt acggcctcgc cactgcccgc caataatgcg
    20761 gatttggccg ctgccggaat gattcggttt tttcatgata aattgctcaa tccgccagat
    20821 aacacatact tgcaggcggt gatgtgaatt aagttaagtg atagactatt tcatcatttt
    20881 tttagctgct atgtacatag cgttaacgct gtgccatgaa gcaacagcga ggagtgtagt
    20941 cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg gctgggcttt ggtcaaaaag
    21001 agcagacccc ggaaaaagag acagaagttc agaatgaaca accggttgta gaagaaatcg
    21061 ttcaggcgca agagcctgcg aaggcttctg aacaagccgt tgaagagcag ccgcaggcgc
    21121 atactgaagc cgaggcggaa acttttgctg ccgacgttgt ggaagtcact gaacaggttg
    21181 ttgaaagtga aaaagcgcag cctgaagcgg aagttgttgc acagccggaa ccggtcgtag
    21241 aagaaacgcc ggagccagtg gctatcgaac gtgaagagct gccgttgccg gaagacgtca
    21301 acgccgaaga ggtttcgcca gaagagtggc aggctgaagc ggaaaccgta gagattgtcg
    21361 aagcggcaga agaagaagcg gctaaagaag aaatcaccga cgaagagctg gaagcacagg
    21421 cgctggctgc cgaagcggca gaagaggcgg tgatggtagt tcctccggca gaagaagagc
    21481 agccggttga agaaattgcc caggagcaag aaaaaccgac caaagaaggc ttcttcgcgc
    21541 gcctgaaacg cagcctgtta aaaaccaaag aaaatctcgg ttccggattt atcagcctgt
    21601 tccgcggtaa aaaaatcgac gatgatctgt ttgaagagct ggaagaacag ctgttgatcg
    21661 ccgatgtggg cgtggaaacc acacgtaaaa ttatcaccaa tctgacggaa ggcgcatccc
    21721 gcaagcagct tcgtgatgcc gaggcgctct atggcctgct gaaagaagag atgggcgaga
    21781 ttctggcgaa agtcgatgag ccgctgaatg ttgaagataa aacgccgttc gtgatcctga
    21841 tggtgggcgt caacggtgtg ggtaaaacca cgacgattgg taagctggcg cgtcagtttg
    21901 agcagcaggg taaatcggtg atgctggcgg cgggcgatac tttccgtgca gctgcggttg
    21961 aacagcttca ggtctggggt cagcgcaaca atattccggt gattgcccag cataccggtg
    22021 cggattccgc ctctgttatc ttcgacgcca ttcaggcagc taaagcgcgt aatatcgacg
    22081 tcctgattgc cgataccgcc ggacgtctgc agaacaaatc gcatctgatg gaagagttga
    22141 agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc gccgcatgaa gttatgctga
    22201 ctattgatgc cagcaccggg cagaacgcgg taagccaggc caaactgttc cacgaagctg
    22261 ttggcttaac cggcatcacg ctaacgaaac tggacggcac ggcgaaaggc ggggtaattt
    22321 tctcggtggc tgaccagttt ggtatcccta tccgctacat tggtgtcggc gaacgtattg
    22381 aggatttgcg tccgtttaag gcggacgact ttatagaggc actttttgcc cgagaggatt
    22441 aacaatgatt cgctttgaac atgtcagcaa ggcttatctc ggtgggagac aggcgctgca
    22501 gggcgttacg ttccatatgc agccgggtga gatggcgttt ctgaccggtc attccggcgc
    22561 agggaaaagt accctcctga agctgatctg tgggattgag cggcccagcg ccgggaaaat
    22621 ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt gaagttccgt ttctgcgtcg
    22681 ccagattggc atgattttcc aggatcacca tctactgatg gaccgtactg tctacgataa
    22741 cgtggcgatc ccgctgatta tcgctggtgc cagcggtgac gatattcgtc gccgggtgtc
    22801 ggcggcactg gataaagtcg ggctactgga caaagcgaag aacttcccta ttcagctttc
    22861 cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg gtgaataagc ccgcggtact
    22921 gctggcggac gaaccgaccg gtaacctgga cgacgcgttg tcggaaggga ttctacgtct
    22981 gtttgaagag tttaaccgcg ttggggtaac cgtattgatg gcaacgcacg acattaacct
    23041 gatttcacgg cgttcctatc gcatgctcac cctgagcgat ggtcacttgc atggaggcgt
    23101 gggccatgaa taagcgcgat gcaatcaatc atattcggca gtttggtggg cgtctggatc
    23161 gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg taacgcaccg aaacgcgcga
    23221 aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt caacgaacag gtgcgctatg
    23281 ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt cgccacgttt ttaacggtga
    23341 tggttatcgc catctccctg acgctgccca gcgtctgtta tatggtgtac aaaaacgtta
    23401 atcaggcggc gacgcagtat tatccgtcac cgcaaatcac tgtttacttg caaaaaacgc
    23461 tggacgatga cgccgctgcg ggcgtggtgg cacagttgca ggccgagcaa ggcgtggaga
    23521 aagtgaacta tctttctcgt gaagacgcac tgggcgagtt ccgtaactgg tctggttttg
    23581 gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc agtggcggtg gtgatcccga
    23641 aactcgattt tcaggggacg gaatcactca atacgctgcg tgatcgtatc acgcagatta
    23701 acggcattga cgaagtgcgg atggatgaca gctggtttgc ccgtctggcg gcgttgaccg
    23761 ggctggttgg gcgcgtttcg gcgatgatcg gcgtgttgat ggtggcggca gtgttcctcg
    23821 tcatcggcaa cagcgtacgt ctgagcatct ttgctcgccg tgactccatc aacgtgcaga
    23881 aactgattgg tgcgacggat ggtttcatcc ttcgcccgtt cctgtacggc ggtgcgctgc
    23941 tgggattttc tggcgcattg ttgtcattaa ttttgtcaga aattctggta ctgcgtttgt
    24001 cgtcagcggt ggcggaagtg gcacaggttt tcggaacgaa gtttgatatc aatggtttat
    24061 cgttcgatga atgcctgcta ttgctgctgg tatgctcgat gattggctgg gtggcagcgt
    24121 ggcttgccac ggtacaacat ttacgccact ttacgcctga ataataaaag cgtgttatac
    24181 tctttccctg caatgggttc cgtagcaggg aaagagaccc cgttgtctct tcccagtatt
    24241 tcatctctat gtcacatttt gtgcgtaatt tattcacaag cttgcattga acttgtggat
    24301 aaaatcacgg tctgataaaa cagtgaatga taacctcgtt gctcttaagc tctggcacag
    24361 ttgttgctac cactgaagcg ccagaagata tcgattgaga ggatttgaat gactgacaaa
    24421 atgcaaagtt tagctttagc cccagttggc aacctagatt cctacatccg ggcagctaac
    24481 gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg ctgaaaagct gcattaccat
    24541 ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc tgcggtttgt tgttcatatt
    24601 gctcgtaatt atgcgggcta tggcctgcca caggcggatt tgattcagga aggtaacatc
    24661 ggcctgatga aagcagtgcg ccgttttaac ccggaagtgg gtgtgcgcct ggtctccttc
    24721 gccgtccact ggatcaaagc agagatccac gaatacgttc tgcggaactg gcgtatcgtc
    24781 aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca acctgcgtaa aaccaagcag
    24841 cgtctgggct ggtttaacca ggatgaagtc gaaatggtgg cccgtgaact gggcgtaacc
    24901 agcaaagacg tacgtgagat ggaatcacgt atggcggcac aggacatgac ctttgacctg
    24961 tcttccgacg acgattccga cagccagccg atggcaccgg tgctctatct gcaggataaa
    25021 tcatctaact ttgccgacgg catcgaagat gataactggg aagagcaggc ggcaaaccgt
    25081 ctgaccgacg cgatgcatgg tctggacgaa cgcagccagg acatcatccg tgcgcgctgg
    25141 ctggacgaag acaacaagtc cacgttgcag gaactggctg accgttacgg tgtttccgct
    25201 gaacgtgtgc gccaactgga aaagaacgcg atgaaaaaac ttcgcgccgc tattgaagcg
    25261 taatttccgc tattaagcag agaaccctgg atgagagtcc ggggtttttg ttttttgggc
    25321 ctctacaata atcaattccc cctccggcaa aacgccaatc cccacgcaga ttgttaataa
    25381 actgtcaaaa tagctattcc aatatcataa aaatcgggta tgttttagca gagtatgctg
    25441 ctaaagcacg ggtagtcatg cataaaacga aataaagtgc tgaacaacaa catcacaaca
    25501 cacgtaataa ccagaagaat ggggattctc aggatgaaca taaagggtaa agcgttactg
    25561 gcaggatgta tcgcgctggc attcagcaat atggctctgg cagaagatat taaagtcgcg
    25621 gtcgtgggtg caatgtccgg tccggttgcg cagtacggtg accaggagtt taccggcgca
    25681 gagcaggcgg ttgcggatat caacgctaaa ggcggcatta aaggcaacaa actgcaaatc
    25741 gtaaaatatg acgatgcctg tgacccgaaa caggcggttg cggtggcgaa caaagtcgtt
    25801 aacgacggca ttaaatatgt gattggtcac ctctgttctt catcaacgca gcctgcgtct
    25861 gacatctacg aagacgaagg cattttaatg atcaccccag cggcaaccgc gccggagctg
    25921 accgcccgtg gctatcagct gatcctgcgc accaccggcc tggactccga ccaggggccg
    25981 acggcggcga aatatattct tgagaaagtg aaaccgcagc gtattgctat cgttcacgac
    26041 aaacagcaat acggcgaagg tctggcacga gcggtgcagg acggcctgaa gaaaggcaat
    26101 gctaacgtgg tgttctttga tggtatcacc gccggggaaa aagatttctc aacgctggtg
    26161 gcgcgtctga aaaaagagaa tatcgacttc gtttactacg gcggttatca cccggaaatg
    26221 gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa ctggaaggtg tggctaacgt
    26281 ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa
    26341 ctacgatcag gttccggcga acaaacccat tgttgatgcg atcaaagcga aaaaacagga
    26401 accaagcggc gcattcgtct ggaccaccta cgccgcgctg caatctttgc aggcgggcct
    26461 gaatcagtct gacgatccgg ctgaaatcgc caaataccta aaagcgaact ccgtggatac
    26521 cgtgatgggg ccactgacct gggatgagaa aggcgatcta aagggctttg agttcggcgt
    26581 atttgactgg cacgccaacg gcacggcgac cgatgcgaag taattgttaa tcggcaactt
    26641 tgggttgccg ccaaattgct aatatcgaat acgttgcttc atgccggatg cggcgtaaac
    26701 gccttatccg gcctacaaga tccaaagaaa tcagtaaatt gcagcacaca ttataggcct
    26761 gataagcgta gcgcatcagg caatttctcg ttggtcatca cttttgaaat caacttgacg
    26821 attaacgctt ctcccagccg ccctgcagtg ccgtaaatcc cagtgcctgc ataaacgccg
    26881 tcatcacacc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acgccaggat
    26941 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    27001 gcacgcgtag agaatccagt gctccctcgg tgccgcttaa ggttacccgc acggccgcaa
    27061 gtagacgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg
    27121 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    27181 ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa atcacaaaag tggaaccagt
    27241 gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca ggtgattaat
    27301 tttttaacag caataattac aaagttaaaa cattagagaa tgaaaaatgt ccagcataat
    27361 cccctgaatg atagtgaatt attccgccct ttgtgccgtt attttatgct gacaaaggca
    27421 cttttttctg tttatctatc aataaattca gaatattatc tgtttttaat cgactgaaaa
    27481 atagggattt taatcgctat catcacaaaa tactgcgcta accccttaat cagacaggca
    27541 aaaacagtgc agtataaaaa aagaacagtc tgatttgtta acacataaaa acaaagcaac
    27601 acaacatcac gaatggggat ttttgactat gaaacggaat gcgaaaacta tcatcgcagg
    27661 gatgattgca ctggcaattt cacacaccgc tatggctgac gatattaaag tcgccgttgt
    27721 cggcgcgatg tccggcccga ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca
    27781 ggcgattaaa gacattaatg ccaaaggggg aattaagggc gacaagctgg ttggcgtgga
    27841 atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa tcgttaacga
    27901 cggcattaaa tacgttattg gtcatctgtg ttcttcctct acccagccag cgtcggatat
    27961 ctacgaagac gaaggtattc tgatgatctc gccgggagcg accaacccgg agctgaccca
    28021 acgcggttat caacacatta tgcgtactgc cgggctggac tcttcccagg ggcctacggc
    28081 ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc gccatcattc acgacaaaca
    28141 acagtatggc gaagggctgg cgcgttcggt gcaggacggg ctgaaagcgg ctaacgccaa
    28201 cgtcgtcttc ttcgacggta ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg
    28261 cctgaaaaaa gaaaatatcg acttcgttta ctacggcggt tactacccgg aaatggggca
    28321 gatgctgcgc caggcccgtt ccgttggcct gaaaacccag tttatggggc cggaaggtgt
    28381 cggtaacgca tcgctgttga acattgccgg tgatgccgcc gaaggcatgt tggtcactat
    28441 gccaaaacgc tatgaccagg atccggcaaa ccagggcatc gttgatgcgc tgaaagcaga
    28501 caagaaagat ccgtccgggc cttatgtctg gatcacttac gcggcagtgc aatctctggc
    28561 aactgccctt gagcgtaccg gcagcgatga gccgctggcg ctggtgaaag atttaaaagc
    28621 taacggtgca aacaccgtga ttgggccgct gaactgggat gaaaaaggcg atcttaaggg
    28681 atttgatttt ggtgtcttcc agtggcacgc cgacggttca tccacggcag ccaagtgatc
    28741 atcccaccgc ccgtaaaatg cgggcgggtt tagaaaggtt accttatgtc tgagcagttt
    28801 ttgtatttct tgcagcagat gtttaacggc gtcacgctgg gcagtaccta cgcgctgata
    28861 gccatcggct acaccatggt ttacggcatt atcggcatga tcaacttcgc ccacggcgag
    28921 gtttatatga ttggcagcta cgtctcattt atgatcatcg ccgcgctgat gatgatgggc
    28981 attgataccg gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt catcgccagc
    29041 gcctacggct ggagtatcga acgggtagct taccgcccgg tgcgtaactc taagcgcctg
    29101 attgcgctta tttccgcaat cggtatgtcc atcttcctgc aaaactacgt cagcctgacc
    29161 gaaggttcgc gcgacgtggc gctgccgagc ctgtttaacg gtcagtgggt ggtgggccat
    29221 agcgaaaact tctctgcctc tattaccacc atgcaggcgg tgatctggat tgtcaccttc
    29281 ctcgccatgc tggcgctgac gattttcatt cgctattccc gtatggggcg cgcctgtcgt
    29341 gcctgcgcgg aagatctgaa aatggcgagc ctgcttggca tcaacaccga ccgggtgatt
    29401 gctttgacct ttgttattgg cgcggcgatg gcggcagtgg ctggcgtact gctcggtcag
    29461 ttctactgcg tcattaaccc ctatatcggc tttatggccg ggatgaaagc ctttaccgct
    29521 gcggtgctcg gtgggattgg cagcattcca ggggcgatga ttggcgggct gatcctgggg
    29581 attgcggagg cgctctcttc tgcctatctg agtacggaat ataaagatgt ggtctcattc
    29641 gccctgctga ttctggtgct gctggtgatg ccgaccggta ttctgggtcg cccggaggta
    29701 gagaaagtat gaaaccgatg catattgcaa tggcactgct ctctgccgcg atgttctttg
    29761 tcctggcggg cgtctttatg ggcgtacaac tggagctgga tggcaccaaa ctggtggtcg
    29821 acacggcttc agacatccgt tggcagtgga tgtttatcgg cacggcggtg gtctttttct
    29881 tccagctttt gcgaccggct ttccagaaag ggttgaaaag cgtttccggg ccgaagttta
    29941 ttctgcccgc gattgatggg tccacggtga agcaaaagct gttcctcgtg gcgctgctgg
    30001 tgcttgtggt ggcgtggccg tttatggtgt cacgtgggac ggtggatatc gccactctga
    30061 ccatgatcta cattatcctc ggtctcgggc tgaatgtggt tgttggtctt tctggtctgc
    30121 tggtgctggg gtacggcggt ttttacgcca tcggcgctta cacttttgcg ctgctcaatc
    30181 actattacgg tctggggttc tggacctgtt tgccgattgc cggactgatg gcagcggcgg
    30241 cgggtttcct gctcggtttt ccggtgctgc gtctgcgcgg tgactatctg gcgatcgtta
    30301 cccttggttt cggcgaaatt gtgcgcatat tgctgctcaa taacactgaa atcaccggcg
    30361 gcccgaacgg aatcagtcag atcccgaaac cgacactctt cggactcgag ttcagccgta
    30421 ccgctcgtga aggcggctgg gacacgttca gtaatttctt tggcctgaaa tacgatccgt
    30481 ccgatcgcgt tatcttcctc tacctggtgg cgttgctgct ggtggtgtta agcctgtttg
    30541 tcattaaccg cctgctgcgg atgccgctgg gccgcgcgtg ggaagcgttg cgtgaagacg
    30601 agatcgcctg tcgttcgctg ggcttaagcc cgcgccgtat caagctgacc gcttttacca
    30661 tcagcgccgc atttgccggt tttgccggaa cgctgtttgc ggcgcgtcag ggctttgtca
    30721 gcccggaatc cttcaccttt gctgaatcgg cctttgtgct ggcgatagtg gtgctcggcg
    30781 gtatgggctc gcaatttgca gtgattctgg cggctgttct gctggtggtg tcgcgcgaat
    30841 tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg tggtttgatg gtgctgatga
    30901 tgatctggcg tccgcagggg ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg
    30961 gcgcagcgaa aggagagcag gcatgagtca gccattatta tctgttaacg gtctgatgat
    31021 gcgcttcggc ggcctgctgg cggtgaacaa cgtcaatctt gaactgtacc cgcaggaaat
    31081 cgtctcgtta atcggcccta acggtgccgg aaaaaccacg gtttttaact gcctgaccgg
    31141 tttctacaaa cccaccggcg gcaccatttt actgcgcgat cagcatcttg aaggtttacc
    31201 ggggcagcaa attgcccgca tgggcgtggt gcgcaccttc cagcatgtgc gtctgttccg
    31261 tgaaatgacg gtaattgaaa acctgctggt ggcgcagcat cagcaactga aaaccgggct
    31321 gttctctggc ctgttgaaaa cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg
    31381 cgccgccacc tggcttgagc gcattggctt gctggaacac gccaaccgtc aggcgagtaa
    31441 cctggcctat ggtgaccaac gccgtctgga gattgcccgc tgcatggtga cgcagccgga
    31501 gattttaatg ctcgacgaac cggcggcagg tcttaaccca aaagaaacca aagagctgga
    31561 tgagctgatt gccgaactgc gcaatcatca caacaccact atcttgttga ttgaacacga
    31621 tatgaagctg gtgatgggga tttcagaccg aatttatgtg gtcaatcagg ggacgccact
    31681 ggcaaacggt acgccggagc aaatccgtaa taacccggac gtgatccgtg cctatttagg
    31741 tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa agtcagcgcc cactacggca
    31801 aaatccaggc gctgcatgag gtgagcctgc atatcaatca gggcgagatt gtcactctca
    31861 ttggcgcgaa cggggcgggg aaaaccacct tgctcggcac gttatgcggc gatccgcgcg
    31921 ccaccagcgg gcgaattgtg tttgatgata aagacattac cgactggcag acggcgaaaa
    31981 tcatgcgcga agcggtagcg attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg
    32041 tggaagagaa cctggcgatg ggcggcttct ttgccgaacg tgaccagttt caggagcgca
    32101 taaagtgggt gtatgaactg tttccacgtc tgcatgagcg ccgtgttcag cgggcgggca
    32161 ccatgtccgg cggtgaacag cagatgctgg cgattggtcg cgcgctgatg agcaatccgc
    32221 gtttgttgtt acttgatgag ccatcgctcg gtcttgcgcc gattatcatc cagcaaattt
    32281 tcgacaccat cgagcagctg cgcgagcagg ggatgactat cttcctcgtc gagcagaacg
    32341 ccaaccaggc gctaaagctg gcggatcgcg gctacgtgct ggaaaacggc catgtggtgc
    32401 tctccgatac cggcgacgcg ctgctggcga acgaagcagt gagaagtgcg tatttaggcg
    32461 ggtaataaca cgttgattga taaggagtca aaagactcct ttgagacagg tgacaaatgt
    32521 aaaattgcct gatgcgctgc gcttatcagg cctactgggt gagttgcaat atattgaatt
    32581 tgcacgatct tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa acgatgagta
    32641 atctgtagag tttgattcag accttctatt tttccgctta tccgtgcccc atctcccatt
    32701 ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttacta tcttacaaat
    32761 gtaacaaaaa agttattttt ctgtaattcg agcatgtcat gttaccccgc gagcataaaa
    32821 cgcgtgaatt cgcgcattcg gtacaacaag agagataaac gatgaaaccg ttacattata
    32881 cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca gtgacgacca
    32941 ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct ctggcccaac
    33001 gttttaacgc cgaaaatccg gattacaaaa ttgtaccgac ctataaaggc aactacgaac
    33061 agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgctccggct attttgcagg
    33121 tttatgaagt tggcaccgcc accatgatgg cgtcgaaagc cattaaaccg gtgtatgacg
    33181 tgtttaaaga ggcagggatt cagttcgatg agtcgcagtt tgtgccgacg gtttccggtt
    33241 actactccga cagcaaaacc ggccacttac tctcccagcc gttcaacagc tcgactcccg
    33301 ttctctatta caacaaagac gccttcaaga aagcggggtt agacccggaa cagccgccga
    33361 aaacctggca ggatctggcg gattattccg caaaactgaa agcctccggt attaagtgcg
    33421 gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc tggaacggtc
    33481 tgccgtttgc cagcaaaaac aacggctttg acggcacgga cgcggtgctg gagttcaaca
    33541 agccggagca ggtgaaacac atcgccatgc tcgaggagat gaacaagaag ggcgacttca
    33601 gctacgtcgg tcgtaaggat gaatccaccg agaagttcta taacggtgat tgcgcgatga
    33661 ccaccgcctc ttccggttct cttgccaaca ttcgcgagta cgccaaattt aactacggcg
    33721 taggtatgat gccttacgac gccgatgcga aagatgcgcc acaaaacgcc attatcggcg
    33781 gagccagcct gtgggtgatg cagggcaaag ataaagaaac gtataccggc gtggcgaagt
    33841 ttctcgactt cctcgcgaag ccagaaaacg ctgctgagtg gcatcagaaa accggttatc
    33901 tgccaattac caaagcggcg tatgacctga cccgtgagca gggcttttac gagaaaaacc
    33961 caggtgcgga taccgcgacg cgtcagatgc tgaacaagcc gccgttgccg ttcaccaaag
    34021 ggcttcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag cttgagagcg
    34081 tgtggaccgg taagaagacg ccacagcagg cgctggatac cgcagttgag cgtgggaacc
    34141 agttactgcg ccgctttgag aaatcgacga agtcttaatc agtgtaatgc cggatgcgtt
    34201 tcgcttatct gacctggcat cgcgtgtagg ccggataagc gaagcgcatc cggcacagtt
    34261 caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc gctcgcgctg gctgccttat
    34321 ctgctggtcg cgccgcagct catcatcacc gttatctttt ttatctggcc tgcgggcgaa
    34381 gcgttgtggt actcgctaca aagcgtcgat ccgtttggtt tctccagcca gtttgtcggt
    34441 ctggataact tcgtcacgct gtttcatgac agctattatc tcgactcctt ctggacgacg
    34501 ataaaattca gcacctttgt caccgtcagc ggtttgctgg tatcgctgtt ctttgcggcg
    34561 ctggtggagt acatcgtgcg cggcagtcgt ttctaccaaa ccttaatgtt gctgccgtat
    34621 gccgtggctc ccgccgttgc cgccgtattg tggatcttcc tgtttaaccc cggtcgcggg
    34681 ctgatcaccc attttctcgc tgagttcggc tacgactgga accacgcgca aaatagcggt
    34741 caggcgatgt ttctggtggt gtttgcctcg gtgtggaagc aaatcagcta caactttctg
    34801 ttcttctatg ccgcgctgca atccattccc cgttcgttga tcgaagccgc agccatcgac
    34861 ggtgcagggc cgattcgccg cttctttaag attgcgctgc cgcttatcgc cccggtgagt
    34921 ttcttcctgc tggtagtgaa ccaggtgtat gccttcttcg acaccttccc ggtgatcgac
    34981 gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa gatctaccgc
    35041 gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt gttgatgttc
    35101 ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt gcgttaccaa
    35161 tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc ctcgggatcg
    35221 cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat aaacaggccg
    35281 tctacgccgc gccgatgacg ctcatccccg gcacgcatct gctggaaaac atccacaaca
    35341 tctgggtgaa cggggtaggc acaaatagcg cgccgttctg gcggatgttg cttaacagct
    35401 ttgtgatggc gttcagcatt acgctcggca aaattactgt ctcgatgctc tcggcatttg
    35461 ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt tttatcaccc
    35521 tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc aacctgaaga
    35581 tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc gccacgtttc
    35641 tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg cggatcgacg
    35701 gtgcgtcacc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa actaatctgg
    35761 cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg ccgttgttga
    35821 ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg atcgccactg
    35881 gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc ctgatcccac
    35941 cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc gatagtgaga
    36001 aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg caaaacctag
    36061 gccattaaac cgctgaccct tgatgtggcg gatggcgaat ttatcgtgat ggtcgggccg
    36121 tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg ggtgaccgaa
    36181 ggcgatatct ggatcaacga ccagcgcgtg accgaaatgg agccgaaaga tcgcgggatt
    36241 gcgatggtgt tccagaatta cgcgctttat ccgcatatga acgtcgaaga aaacatggcg
    36301 tgggggctga aaattcgcgg catgggcaag cagcaaatag ccgagcgcgt taaagaagcg
    36361 gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgcgagct ttccggcggt
    36421 cagcgccagc gtgtggcgat gggacgcgcg attgtgcgcg atccggcggt gttcttgttt
    36481 gatgagccac tctctaacct cgatgccaag ctgcgcgtac agatgcgtct tgaactgcaa
    36541 cagctgcacc gtcgcctgaa aacgacttca ctctacttta ctcacgatca ggttgaggcg
    36601 atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca gattggcacg
    36661 ccggtggaag tctacgaaaa gcccgccagc ctgtttgtgg cgagttttat cggcagcccg
    36721 gcgatgaacc tgctgacagg ccgcatgaat aatgaaggca cgcatttcga actggacggc
    36781 ggtatcgtgc taccgctaaa cggtggctac cgtcagtatg ccgggcgtaa aatgaccctc
    36841 ggcattcgcc cggaacatat tgcgctaagc tcgcaagcag aagctggtgg tgcgactggc
    36901 gcatcaggag cgcccgacgg caggcagcac gctgtggctg catctggcgg aaaatcagct
    36961 gcatcttttt gatggtgaaa caggacaacg agtatgagta actggcctta tccccgcatc
    37021 gtcgctcatc gtggcggcgg taagctggcc ccggaaaaca ccctggcggc aatcgacgtc
    37081 ggggcaaaat acggtcataa gatgatcgaa tttgacgcga agttatcgaa agatggcgag
    37141 atcttcctgc tccatgacga caatctcgaa cgcaccagca acggctgggg cgtcgcgggt
    37201 gaactgaact ggcaggattt actgcgcgtg gatgcgggca gttggtacag caaagcgttt
    37261 aaaggtgagc cgctgccgtt gctttcgcag gtggcggaac gctgtcgcga acacgggatg
    37321 atggcgaata tcgaaatcaa acccaccact ggcactggac cattaacggg caaaatggtg
    37381 gcgctggcgg cacgccaact gtgggccggt atgacgccgc cgctgctgtc atcgtttgag
    37441 attgatgctt tagaagctgc acaacaggcg gcaccggaac tgccgcgtgg tttattgctt
    37501 gatgagtggc gcgacgactg gcgcgaactg accgcgcggc tgggctgcgt ctctattcat
    37561 ctcaatcata agttactcga taaagcgcga gtgatgcagt tgaaagacgc cggattacgg
    37621 attctggttt ataccgtcaa taaaccccag cgcgcggcag agttgctgcg ctggggcgtg
    37681 gattgcatct gtaccgatgc gattgacgtg attggtccga actttacggc ccaatagttt
    37741 tcaacggaat gtcaggctgc ggcgtgctgg gctgattcag catgtcgccg tttctcctct
    37801 ccggcagcat atgctgctga ttcaacgaac tatccggatt acggttgctg tttaacatcc
    37861 cgccgttggt gttgggcagc atttgctgcc gggcgggatt tcgctccccc ggctgtgact
    37921 gcaacacccg ctgagaatta ttgtttatct ggttttctaa atgctgctgt tgcaactgag
    37981 tttgcgtttt cagttgctga ttcagcatcc ctttttgctg gatttgctga gtctgcatct
    38041 gggtttgcat ccgctgctgg ctgggtatct gataccccgg ctggttaggg ttgttcagag
    38101 tattaatggg ctgtgcaaag ccgacaaacg gcaggagtgc cgtaagaatc agaagtcgtt
    38161 tcatcgcgta tcctcctctg aagatatcct ttaagtttac tcgcttcccg acaaaacgat
    38221 gattaattca gagttatata ccaggcttag ctggggttgc cccttaatct ctggagaata
    38281 acgatgataa aaccgacgtt tttacgccgg gtggccattg ctgctctgct ctcaggaagt
    38341 tgttttagcg ccgccgccgc gcctcctgcg ccgcccgtct cgtatggtgt ggaggaagat
    38401 gtcttccacc cggtacgcgc gaaacaggga atggtagcgt ctgtggacgc cactgccact
    38461 caggtggggg tggatatcct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg
    38521 ggctacgcgc tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg
    38581 ttaatccgct cgaaaaatgg caataccacg gctatcgatt tccgcgaaat ggcacccgcc
    38641 aaagcgaccc gcgatatgtt cctcgatgat cagggcaacc cggacagcaa aaaatcactc
    38701 acttcgcatc tggcttccgg cacaccgggt acggtagcag gtttctcgct ggcgctggat
    38761 aaatacggca ccatgccgct gaacaaagtc gtgcagcccg cgtttaaact ggcacgcgat
    38821 ggttttatcg ttaacgacgc gctggctgac gatctcaaaa cctacggtag cgaagtgttg
    38881 ccgaatcacg aaaacagtaa agctatcttc tggaaagagg gcgagccgct gaaaaagggc
    38941 gacacgctgg tgcaggcgaa cctggcaaag agcctggaga tgattgctga aaacggcccg
    39001 gacgaattct ataaaggcac gattgcggaa cagatcgccc aggagatgca gaaaaacggt
    39061 ggcttgatca ctaaagaaga tttagcggcc tataaagcgg tcgaacgcac tccgataagc
    39121 ggcgattatc gcgggtatca ggtttactcc atgccaccgc catcctccgg cgggatccat
    39181 atcgtacaaa tcctcaatat tctggaaaac ttcgatatga agaaatacgg ctttggcagc
    39241 gccgatgcga tgcaaatcat ggcagaagcg gagaaatacg cctacgccga ccgctcggaa
    39301 tatcttggcg acccggattt tgtcaaagtc ccgtggcagg cactgaccaa taaagcctat
    39361 gccaaatcca ttgccgatca aattgatatc aacaaagcga agccgtccag tgagattcgc
    39421 cctggcaagc ttgcgcctta tgagagtaat caaactaccc attactctgt agtggataaa
    39481 gacggtaacg cggtggcggt gacctatacg ctgaacacca ccttcggtac gggtattgtc
    39541 gcgggcgaga gcggtattct gcttaataac cagatggatg atttctccgc caaaccgggc
    39601 gtaccgaacg tttacgggct ggtgggcggt gatgccaacg ccgtcgggcc gaacaaacgc
    39661 ccgctgtcgt cgatgtcgcc gaccattgtg gtgaaagacg gtaaaacctg gctggttacc
    39721 ggtagcccag gcggtagccg gattattacc acggtgctgc aaatggtggt gaatagcatc
    39781 gattatggca tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg
    39841 ccggacgagc tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca
    39901 aaaggtcaga aagtggcgct gaaagaggcg atgggcagca cacaaagcat tatggttggg
    39961 ccggacggtg agttgtacgg cgcatccgac ccgcgctcgg tggatgattt aacggcgggg
    40021 tattaatatc ttaagccctc ccggtaggtg agggcttaaa tgacaaactt agattgcaaa
    40081 ttctctattt cacccgcgcc atataatacg catcgacata ttcaccatta cgcaatgcgt
    40141 acttcttacc ggtcccttca atctcaaagc cgaatttttt atagacctta attgctggcg
    40201 cgttatcgac aaacacggtt agttcaatgc gatctacccg taaccagttg tcgcacattt
    40261 caatcatctc tcgcatcagg gcgctggcga cgccgcggtt ct
//
LOCUS       DADKXM010000019        40183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.019, whole genome shotgun sequence.
ACCESSION   DADKXM010000019 DADKXM010000000
VERSION     DADKXM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.019"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..524)
                     /locus_tag="JQR05_001037"
                     /pseudo
     CDS             complement(1..524)
                     /locus_tag="JQR05_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            complement(982..1068)
                     /locus_tag="JQR05_001038"
     tRNA            complement(982..1068)
                     /locus_tag="JQR05_001038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Leu,seq:gag)
     gene            complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JQR05_001039"
     CDS             complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JQR05_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY5323253.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLTTLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JQR05_001040"
     CDS             complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JQR05_001040"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY5323254.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JQR05_001041"
     CDS             complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JQR05_001041"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY5323255.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JQR05_001042"
     CDS             complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JQR05_001042"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY5323256.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMPEQLGDK"
     gene            complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JQR05_001043"
     CDS             complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JQR05_001043"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY5323257.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6700..6993
                     /gene="yhbY"
                     /locus_tag="JQR05_001044"
     CDS             6700..6993
                     /gene="yhbY"
                     /locus_tag="JQR05_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY5323258.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JQR05_001045"
     CDS             complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JQR05_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY5323259.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7873..9306
                     /gene="dacB"
                     /locus_tag="JQR05_001046"
     CDS             7873..9306
                     /gene="dacB"
                     /locus_tag="JQR05_001046"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY5323260.1"
                     /translation="MRFSRFIIGLTSCIAFRVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JQR05_001047"
     CDS             complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JQR05_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY5323261.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDGEDWDEDDEEGVEFIYKR"
     gene            complement(10668..11633)
                     /locus_tag="JQR05_001048"
     CDS             complement(10668..11633)
                     /locus_tag="JQR05_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5323262.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JQR05_001049"
     CDS             complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JQR05_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY5323263.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JQR05_001050"
     CDS             complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JQR05_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY5323264.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12608..13579
                     /gene="ispB"
                     /locus_tag="JQR05_001051"
     CDS             12608..13579
                     /gene="ispB"
                     /locus_tag="JQR05_001051"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY5323265.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13807..14085
                     /gene="sfsB"
                     /locus_tag="JQR05_001052"
     CDS             13807..14085
                     /gene="sfsB"
                     /locus_tag="JQR05_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY5323266.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14133..15392)
                     /gene="murA"
                     /locus_tag="JQR05_001053"
     CDS             complement(14133..15392)
                     /gene="murA"
                     /locus_tag="JQR05_001053"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY5323267.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15447..15701)
                     /gene="ibaG"
                     /locus_tag="JQR05_001054"
     CDS             complement(15447..15701)
                     /gene="ibaG"
                     /locus_tag="JQR05_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY5323268.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15861..16154)
                     /gene="mlaB"
                     /locus_tag="JQR05_001055"
     CDS             complement(15861..16154)
                     /gene="mlaB"
                     /locus_tag="JQR05_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY5323269.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16154..16789)
                     /gene="mlaC"
                     /locus_tag="JQR05_001056"
     CDS             complement(16154..16789)
                     /gene="mlaC"
                     /locus_tag="JQR05_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY5323270.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QVYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16808..17359)
                     /gene="mlaD"
                     /locus_tag="JQR05_001057"
     CDS             complement(16808..17359)
                     /gene="mlaD"
                     /locus_tag="JQR05_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY5323271.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17364..18146)
                     /gene="mlaE"
                     /locus_tag="JQR05_001058"
     CDS             complement(17364..18146)
                     /gene="mlaE"
                     /locus_tag="JQR05_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY5323272.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18154..18963)
                     /gene="mlaF"
                     /locus_tag="JQR05_001059"
     CDS             complement(18154..18963)
                     /gene="mlaF"
                     /locus_tag="JQR05_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY5323273.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19173..20150
                     /locus_tag="JQR05_001060"
     CDS             19173..20150
                     /locus_tag="JQR05_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY5323274.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20164..21150
                     /gene="kdsD"
                     /locus_tag="JQR05_001061"
     CDS             20164..21150
                     /gene="kdsD"
                     /locus_tag="JQR05_001061"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY5323275.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRNVLLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21171..21737
                     /gene="kdsC"
                     /locus_tag="JQR05_001062"
     CDS             21171..21737
                     /gene="kdsC"
                     /locus_tag="JQR05_001062"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY5323276.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21734..22309
                     /gene="lptC"
                     /locus_tag="JQR05_001063"
     CDS             21734..22309
                     /gene="lptC"
                     /locus_tag="JQR05_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY5323277.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22278..22835
                     /gene="lptA"
                     /locus_tag="JQR05_001064"
     CDS             22278..22835
                     /gene="lptA"
                     /locus_tag="JQR05_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY5323278.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22842..23567
                     /gene="lptB"
                     /locus_tag="JQR05_001065"
     CDS             22842..23567
                     /gene="lptB"
                     /locus_tag="JQR05_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY5323279.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23615..25048
                     /gene="rpoN"
                     /locus_tag="JQR05_001066"
     CDS             23615..25048
                     /gene="rpoN"
                     /locus_tag="JQR05_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY5323280.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25071..25358
                     /gene="hpf"
                     /locus_tag="JQR05_001067"
     CDS             25071..25358
                     /gene="hpf"
                     /locus_tag="JQR05_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY5323281.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25476..25967
                     /gene="ptsN"
                     /locus_tag="JQR05_001068"
     CDS             25476..25967
                     /gene="ptsN"
                     /locus_tag="JQR05_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY5323282.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26013..26867
                     /gene="rapZ"
                     /locus_tag="JQR05_001069"
     CDS             26013..26867
                     /gene="rapZ"
                     /locus_tag="JQR05_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY5323283.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26864..27136
                     /gene="npr"
                     /locus_tag="JQR05_001070"
     CDS             26864..27136
                     /gene="npr"
                     /locus_tag="JQR05_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY5323284.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27349..27981
                     /gene="yrbL"
                     /locus_tag="JQR05_001071"
     CDS             27349..27981
                     /gene="yrbL"
                     /locus_tag="JQR05_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY5323285.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27978..28706)
                     /gene="mtgA"
                     /locus_tag="JQR05_001072"
     CDS             complement(27978..28706)
                     /gene="mtgA"
                     /locus_tag="JQR05_001072"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY5323286.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGSEPFMQQHQLD"
     gene            complement(28703..29356)
                     /gene="elbB"
                     /locus_tag="JQR05_001073"
     CDS             complement(28703..29356)
                     /gene="elbB"
                     /locus_tag="JQR05_001073"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY5323287.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29586..31922)
                     /gene="arcB"
                     /locus_tag="JQR05_001074"
     CDS             complement(29586..31922)
                     /gene="arcB"
                     /locus_tag="JQR05_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY5323288.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKNNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32018..32947)
                     /locus_tag="JQR05_001075"
     CDS             complement(32018..32947)
                     /locus_tag="JQR05_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY5323289.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGIRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33622..38082
                     /gene="gltB"
                     /locus_tag="JQR05_001076"
     CDS             33622..38082
                     /gene="gltB"
                     /locus_tag="JQR05_001076"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY5323290.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDMTFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38095..39513
                     /locus_tag="JQR05_001077"
     CDS             38095..39513
                     /locus_tag="JQR05_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5323291.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39697..40176
                     /locus_tag="JQR05_001078"
     CDS             39697..40176
                     /locus_tag="JQR05_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY5323292.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT         9961 a        10845 c         9923 g         9454 t
ORIGIN      
        1 atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata
       61 aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa
      121 catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat
      181 aagggatata catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa
      241 tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca
      301 tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg
      361 atatacgttt aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg
      421 caccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc
      481 aatgagattt aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc
      541 acaaccaata taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact
      601 ctgatccata tcagaccgaa acactgttag tttttctttt taaatgatgc gaccattgcg
      661 ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat
      721 cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt
      781 accggaactg gagttgacac aataaagtgc caattatgtc agcagtaggg aaaagtttat
      841 aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg
      901 agatacagat acctgacaac cattcatcag accaggacca aaacgaaaag agacgctttt
      961 cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta agccctattg
     1021 ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact
     1081 ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggctgagt ttgttcggtt
     1141 ttcgccggtg cactcagatt ttcccattcg ctacctctat tggttttgtt gctattgatg
     1201 ttacccagca ccagactgat gatgaagaat aacgttgtca gcagcgccgt catgcgggtc
     1261 atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag
     1321 gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc
     1381 acaataagga aaactactaa aagagcttcg tacataatca acctgttcct tgcggagttg
     1441 ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg
     1501 tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac
     1561 tgcggaaaaa aacagcaaaa ccgatgtgtt cgccgagaaa aaaggcgaca atcgccgcct
     1621 ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg cggtgtgcaa
     1681 attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc
     1741 cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca
     1801 cggctttaac tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgtacgttaa
     1861 ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac
     1921 gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat
     1981 ccagcaggat cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt
     2041 tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct aactgtttca
     2101 gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca cgcagctggc
     2161 cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg actttattgc
     2221 cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg
     2281 ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat
     2341 tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc agttcgcgca
     2401 gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca atcttcagtt
     2461 cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcgg
     2521 catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg atctcctttt
     2581 ccatttccgc ttcgatggcc tcttctaccg catccggcag tttagtgccg tcgatagaga
     2641 agaatttaat cccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct
     2701 ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga
     2761 gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag
     2821 aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc agcactttac
     2881 ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac
     2941 cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca
     3001 gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga
     3061 atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc
     3121 tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat acccaccaac
     3181 agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag
     3241 agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc
     3301 tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca
     3361 tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt
     3421 aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg gatatcatta
     3481 ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatcgacc
     3541 gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac
     3601 tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg
     3661 atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat cagcgagtta
     3721 tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga
     3781 tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc cctggtatga
     3841 attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg
     3901 atgcgggaac ttacttgtcg cctaactgct ctggcatggt gttacccggg ttcggcgtac
     3961 gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag
     4021 cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa
     4081 tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat
     4141 ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca
     4201 cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac
     4261 ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga
     4321 agccccactg agtcaccatg ttacgtgcca ggttggtcgc aactttaata tcgttggacg
     4381 caccggtaga aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca
     4441 gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca
     4501 agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcacttta tgcaccggat
     4561 cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa gccgttgatt
     4621 ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg attttgtctt
     4681 tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg
     4741 ccgcttcgtt caccaggttc gctaggtcag caccggagaa accaggagta ccacgggcaa
     4801 tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct
     4861 gctcacgacc gcgaacatct ggcaagccga ccacaacctg gcggtcgaaa cggccaggac
     4921 gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg atgatacctt
     4981 cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat
     5041 cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa
     5101 agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac
     5161 ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg
     5221 cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca
     5281 ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact
     5341 ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg
     5401 ttttgatctg atcttctgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt
     5461 tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca
     5521 tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga
     5581 caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt
     5641 aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg
     5701 cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc
     5761 cattagactc gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca
     5821 gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg
     5881 ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc
     5941 ccggttaggg tttacgcccg gtcgctacaa tatacacttc tcgcgaacgt gcacgagaag
     6001 agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat
     6061 cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac
     6121 gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc accgccggtg
     6181 ttccgctcat gtttggtgcc atatcagaca tgacaacctg gactttgctg tcgccaacgc
     6241 gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac
     6301 caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt
     6361 gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc
     6421 ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccaa gcacgggaac
     6481 gtaatccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc
     6541 ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat
     6601 cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag
     6661 aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa
     6721 aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat
     6781 ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc
     6841 aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg
     6901 cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca
     6961 actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc
     7021 tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga
     7081 taaaaggccg cagagcggcc tttttccttt ctttacaata catcaacatc ttgagtattg
     7141 ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc gcccggcgtt
     7201 ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat aggcgagttt
     7261 acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt
     7321 tcttcgtcag aatccagatt cagcaccgtt acggtagcac caaaaataac gcgcccattg
     7381 ttgggcattt tggtgacatc aatcacctgc gcgtttgaca gcttggcttc gatgtctttg
     7441 atacggcctt cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc gttttctttc
     7501 aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg gcgcacagat
     7561 ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct
     7621 tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca ccccggctgt
     7681 tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc gacccgggta
     7741 caagtcccag gtcagctaca attcacattt tgatggtcat tttaccctga agttcccgaa
     7801 gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat tccaggttgt
     7861 tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg tatagcgttc
     7921 agggttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgcagg tgccaacctt
     7981 gccctgatgg tgcaaaaagt cggtgcgtcg gcccccgcta ttgattacca cagtcagcag
     8041 atggcgctgc ccgccagtac ccagaaagtg attactgcgc tggcggcgtt gattcaactc
     8101 ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta
     8161 cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg tcaggatatt
     8221 cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg
     8281 atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg gaatgacatg
     8341 acacaatgct ttagcgctcc gcctgctgcc gccatagttg accgcaactg tttctccgtc
     8401 tcgctctaca gtgccccaaa gcctggtgat atggctttta tacgcgtggc atcttattac
     8461 cccgttacga tgttcagcca ggtacgcacc ctcccccgtg gttctgccga agcgcaatac
     8521 tgcgaactgg atgtggtgcc aggcgacctg aaccgcttta cgctgacggg atgcctgcca
     8581 caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag ctatgccggt
     8641 gcaattctga aagatgagtt aaaacaggcg ggtatcacct ggagcggaac actgctgcgc
     8701 cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc cccgctgcac
     8761 gatctgctca agattatgct gaaaaagtcg gacaacatga tcgccgatac ggttttccgc
     8821 atgatcggcc atgcgcgctt caatgtgcct ggaacatggc gggctgggtc ggacgccgtg
     8881 cgtcagatcc tgcgccagca agccggtgtc gatattggta acaccattat tgccgatggt
     8941 tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt gctgcaatac
     9001 attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc gggctatgac
     9061 ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggcaa agtctcagcg
     9121 aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac agcgagcggg
     9181 caacgaatgg catttgtaca atatctttct ggctatgcag tagaacccgc ggatcagcgt
     9241 aatcgccgta ttccgttagt gcgttttgaa agccgtttgt acaaagatat ttatcagaac
     9301 aattagtcaa aaagaaaccc cggcgcatgg ccggggtttc agattattga caaagtgcgc
     9361 tttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatcg tgcaaattca
     9421 atatattgca atcatcttgt aggcctgata agtgtagcgc atcaggctta tttggcattg
     9481 gtcattagtg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag
     9541 tcttcaccat cctcttcagc aatctcttca agctgctgac ggtgataatc atcccacatg
     9601 aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata
     9661 aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga
     9721 taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc ttcggcttct
     9781 gccttatcca gcagatcgat cttgttgaag actaaccaac gcggtttcgc cgccagatcc
     9841 tggctgtatt tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg
     9901 tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg
     9961 aagcgaatgc ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca
    10021 acaacgaagc tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta
    10081 aacggataat ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta
    10141 cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc
    10201 tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat
    10261 ttgaaacggg tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg
    10321 tgtttagtca tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc
    10381 ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac aatcgcggct tgcgccgttc
    10441 tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc
    10501 tcgtcggctt ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct
    10561 ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg
    10621 accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg
    10681 cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg
    10741 gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac
    10801 atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta
    10861 aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct
    10921 tccgccaggg cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc
    10981 tgccagtggc taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta
    11041 cataaagtgt acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt
    11101 tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag
    11161 gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccaga
    11221 ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata
    11281 aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc
    11341 ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc
    11401 gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc
    11461 ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg
    11521 attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag
    11581 caaattgctg tggtaagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat
    11641 tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga
    11701 atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact
    11761 tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact
    11821 ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg
    11881 tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg
    11941 aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca
    12001 gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt
    12061 tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa
    12121 acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc
    12181 cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt
    12241 cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac
    12301 cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg
    12361 cttctgcgca caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg
    12421 aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa
    12481 cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaattac cctatattcc
    12541 taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc
    12601 ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa
    12661 tgcggcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta
    12721 catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc
    12781 tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt ttatccacac
    12841 ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac
    12901 cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg
    12961 cgcgttccag atgatgacca gcctcggttc actcaaagtg ctggaagtca tgtcagaagc
    13021 tgtaaacgtc atcgcagaag gtgaagttct gcaactgatg aacgttaacg atccggacat
    13081 cactgaagaa aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc
    13141 cgcgcagtgt tccgggattc tggctggctg tacgccggag gaggagaaag gcctgcagga
    13201 ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc
    13261 cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct
    13321 gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc
    13381 catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg
    13441 tggatctctt gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc
    13501 gttacaggtg ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc
    13561 tgttcaacgc gatcgttaat cccctccctt catcccgcgc agtgcgcggg atgattccag
    13621 taaattccat aaaaacacta attcagcgct ataacacctg aaaaaaaggg cgatgaatct
    13681 ttatatattc tgaatattca cactctttac aggaactttt tagcgcaata ggccatcagg
    13741 agtatagtga tgcccgacag aagaagtgtt ctgaatgaaa gcgactaact taaggagtga
    13801 ggaaagatgg aaagtaattt cattgactgg catcccgctg acatcattgc aggtttgcgc
    13861 aagaaaggaa cgtcaatggc ggcggaatct cgcagaaatg gtttgagttc ctcaacgctg
    13921 gcaaacgcat tatcgcgccc atggccgaaa ggagagatga ttattgcgaa agccctggga
    13981 actgacccct gggttatctg gccatcacgc taccatgatc cgcagaccca tgagtttatc
    14041 gacagaacgc agttgatgcg tagctacact aaaccgaaaa aatgagtggt ctggcggtag
    14101 ccccgcgaac ggggctgcca gctctcagac gattactcgc ctttcacacg ctcaatattt
    14161 gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc cacgatcgat gtgataaata
    14221 cgatcaacca ccgtcgtccc ttccgcaata cagccagcca gcaccaggct tgctgatgca
    14281 cgcagatcgg ttgccataac ctgtgcacca gaaagttttt caacaccatg acaaataacg
    14341 gtattgcttt cgatttcggc gtgcgcgccc atacggctca gctctggcac atgcataaag
    14401 cggttttcaa agaccgtttc ggtgatgaac ccggtccctt cagccaccag gttcaacagc
    14461 gtgaactggg cctgcatatc ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca
    14521 gccttcggac gtttgccatg catatccagg ctaatccagt cttcgccgac ttcgatgtcc
    14581 gctccagcat cacgcagttt cgccagcacg gcgtccagag tatctggctg cgcgttacgg
    14641 cagataattt tgccacgaga gatcgccgcc gccaccagga aagtaccggt ttcgatacga
    14701 tccggcagca cgcgatagac accgccgcct aaacgttcca caccttcgat gacgatacga
    14761 tcggtgccct gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg
    14821 atttccggtt cacgcgctgc gttttcaata atcgtggtgc cttccgccag ggttgcagca
    14881 cacatgatgg tcaccgttgc gccaacgctg actttatcca tcacgatatg tgcgcctttc
    14941 agacgaccat cgacggaagc tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat
    15001 tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca accgccaggt
    15061 agtgaaactt gcccctgacc aaagcgcgct accagcggcc ccagcgccca gatagaagca
    15121 cgcatggttt taaccagatc gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata
    15181 tgcacagaac cattacgttc tactttcgca cccagctggc ttagcagttt cattgatgta
    15241 tcgacgtctt tcagtttcgg gacgttctgg atctctaccg gttcttccgc cagtagtgcg
    15301 gcaaaaagaa taggcagagc agcattttta gcgccggaaa ttgtgacttc gccctggagc
    15361 ttcgttggcc cctgaacacg aaatttatcc atttagtttg ttctcagtta acaattcata
    15421 tccgctaccg gcgaatcgcc catagctcaa aagccgttca gtttgcgatc gcgcgcccac
    15481 tccgcagggg tatacgcttt gatcgacaca gcatgaatgc ggttatccgc aatatattcc
    15541 atcagcgggc catagaccgt ctgctgtttt ttaacccgac tcataccgtc aaacaactca
    15601 cccacggcaa taacctgaaa gtggctgcca tcgccggaaa cgtggacttc ctggagggag
    15661 agagcgttca tcaacacgct ctgaatttca ttattttcca tgggatcttc aatcatcagt
    15721 taataaacca gcgaaacatc ttagagcaaa gttgcgctgg cataaataag caaaaagcct
    15781 cgctgataaa tcagacaagg ctcgacttgc aggcaggttt gccggacagg cggttaacgc
    15841 catatccggc ctgaaaaaat ttaacgaggc agaacatcag caggcaaatt atacaatttc
    15901 gcgagggtat acactttgtc gtttaccccc tgaagcgtca cgttgttgcc ctgctttttc
    15961 gccagatcga taagatggag cagcagtgcc agtccccccg tatccacgcg ggacacacgg
    16021 ctaagatcga tgcaggtaat ccccttcacc gcttcctcac gcatttccca aagcggtagc
    16081 agaacgtcct gatccagctc tccggataac gccagcgtgt cacccgtctg catccagctc
    16141 agtgactcgc tcattatttt ttctcttcca gagtgatttt ctgttgagaa atcgatttca
    16201 gttgcgcagt caggccgtcg atacctttgg tacgcagcag cgttccccac tcgttttgtt
    16261 tggtggtgat catactgacg ccttcagcaa tcatgtcgta agcttgccag tttcccgtct
    16321 gggagttttt acgccactgg aagtccagac gcaccggcgg acggccattc gggtcaataa
    16381 tggtaacgcg aataggcaca atggttttat cgcccagcgg ctgttctggc gcaatctgat
    16441 aggtttgacc gtgatacatc gctagcgcct gaccgtaaac ctgcttcagg tactcacgga
    16501 aagcggcaaa gtaggcttca cgttgagcag gggtcgcact cttgtaatac tggcccagca
    16561 ccagcgcacc ggcgtatttc acctgtacgt atggcagcag ttcctgatca acaatggtac
    16621 gcagataatc cgggttggcc cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt
    16681 tctgcgccgc ctcgtccatc agcttataag gattggtctg gtctgccgcg gttgccgcac
    16741 tcagaggtgc aatcaccagc aaagcgacca tcattaaacg tttaaacatg cgtcggttct
    16801 cctgaaatta tttcgttgta cccacaggtt cagtggtttc attattacct ggcgcagcag
    16861 ctggcgcatc gccactattc ttattgtcat cgcctttact accgtaaagg aactgaccaa
    16921 tgagatcttc cagcaccatc gcagacttgg tgtcctgaat tgtatcgcca tccttcagga
    16981 tagcagtccc cagttccggg tcttcaaaac cgacgtttaa tgccagatat tgttctccca
    17041 gcaggccgga agtacgaatg ctcagcgaac tggtatcagg aatgtggtta taacgttgtt
    17101 caatttccag cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata tccgccaccc
    17161 ggcccacaac aacaccacca atgctgaccg gagagcgcgc tttcaggccg ccaatgttat
    17221 cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat ggacgtcacg ttcgccgcct
    17281 tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa aaagataccc acccaaattt
    17341 catttttttt cgtttgcatg aactcaattc ccaaacatca atgcggtcag cacaaaatcc
    17401 agccccagaa cagccagaga cgagtggaca acggtgcgag tggttgcccg gctaatcccg
    17461 gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg
    17521 aacaccacgc tcttaatcag acagttgacc agatccatac gccagtcgac ggcattttgc
    17581 attgccgacc agaagaaccc gctatcaatg cctttccaac tgacgccgac cagcgatccg
    17641 ccccagatcc ccacggcgac gaagataacc gtcaacagtg gtaatgaaat aaccccagcc
    17701 cagaaacggg gagaaataac ccgacgcagc ggatccaccg ccatcatctc catactggag
    17761 agttgctctg tagcgcgcat caggccgatt tctgcggtta acgccgaacc agcacgcccg
    17821 gcaaacaaca gcgcggcaac caccggcccc agttcacgca gtagcgataa cgccaccagc
    17881 atacccagac tggtttccgc gctataagtg gtcagaacca gataaccttg cagccccaac
    17941 accattccga tgaacacgcc agaaactaca ataatcagca tcgacaggac gccgacatta
    18001 tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca
    18061 ttgaataaca ttaacccggc ccgcccgaac gttctcaggg ttttaatccc tttatgtccg
    18121 agcgacgcca gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg
    18181 ataatcgccg gcaggatagc ggaacggaac aggcccgtca gctatcccgt ccagaaactg
    18241 acgtacgcgc ggatcaggat tcgtctgcaa cgcctgggca ctgccatgag cgacaatttt
    18301 tttgtccgcc aggatccagg cgtgatccgc aatacttaac acttccggca catcgtgaga
    18361 aaccaccaca caagtcacgc ccagcgcgct gttcagctca gaaatcagct tcaccagtac
    18421 gcccatggtg atgggatctt gcccaacaaa aggttcatca aacatgatga gatccggctc
    18481 cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg
    18541 cattagttta gccgctccac gcagccccac ggcctccagc ttcatcatca ccgtactatg
    18601 caacaatggc gcgggaagtt gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac
    18661 gttcatatca gtgaacaacg ccccggactg aaataacatg ctcatccgtt tacgcactgt
    18721 atacaggcgc gaacgagaca tcgccggaat attctcacca tcgaaaagga tctcaccatg
    18781 atctggtgcg atttgcccgc caatcagacg gagtagcgtc gttttaccga tgcccgatgg
    18841 ccccatgatc gccgtgatct tccctcgcgg cacggtcagg gaaatattat cgaagatgca
    18901 gcgattgcca cgcgtaaaac tgacatcgcg catatcgact aaattcgcca cagactgctc
    18961 cataattcac ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt taaccttgaa
    19021 cccaacatat ttacagaata ttacccgccg tggttagcga aagctggcat ttgttttact
    19081 ttttagccgc ataaagtcaa aattaagaat ccgttacggc tttctgaaaa tcttcagcgg
    19141 accggcgagt atacctgaag aaaggacgtt agatgctttt agctacggca ctgttaattg
    19201 ttggtttact tttggtcgtt tacagtgccg accgcctggt ttttgccgcg tctattcttt
    19261 gccgaacctt tggcatcccg ccgctgatca tcggcatgac ggtggtcagt attggtacat
    19321 cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca cgaacaacgc gatttagccg
    19381 ttggtacagc cctcggctca aacattatca atatattgct gatcctcggt ctggctgcgc
    19441 tggttcgtcc ttttaccgtc cattccgatg ttctgcgccg tgaattaccc ttaatgttgt
    19501 tggtcagcgt ggtggccggt tccgtactct atgacggaca acttagtcga agcgatggta
    19561 tctttctcct ctttctggct gtgctatggc tgctgttcat tgttaaactt gcacgtcagg
    19621 ctgaacgtca ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg
    19681 gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca
    19741 cgcggatggt ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga
    19801 cgatgggtct gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag
    19861 cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt
    19921 ttaatattgt catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac
    19981 tggcgtacag tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt
    20041 gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta
    20101 tcgtatggct ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc
    20161 attatgtcgc acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc
    20221 ctggcgattg aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg
    20281 cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa
    20341 tcggggcaca tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt
    20401 ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg
    20461 gtgattgcta tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt
    20521 aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc
    20581 gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca
    20641 ccgaccagca gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta
    20701 aaagcacgcg gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt
    20761 cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt
    20821 aagaaaacgg ccagtctgcg taacgtattg ctggaagtta cccgcaaaaa tcttggtatg
    20881 actgtcattt gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc
    20941 cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg
    21001 gggggaatac gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc
    21061 cgccatatca cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg
    21121 catgatttac tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag
    21181 caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag
    21241 agaacattcg tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata
    21301 tgggcaataa cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt
    21361 gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag
    21421 aagatcgttg tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga
    21481 tcgcctttag cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg
    21541 gcgatgatct catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg
    21601 atgcgcatcc actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg
    21661 gcgcagtgcg cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca
    21721 aagggcaatc gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt
    21781 tctggtgatg atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa
    21841 caacaatgat cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg
    21901 ggcactaagc tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc
    21961 gtggtttacg cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt
    22021 aaaagcagat aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga
    22081 agtcaacgca cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat
    22141 caatctggtg acacaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt
    22201 taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga
    22261 aaaggttaga acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg
    22321 ccagctcact tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc
    22381 cgatccacat tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta
    22441 ccggtaatgt catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta
    22501 cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt
    22561 tctaccagat gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg
    22621 aactggcaaa agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca
    22681 acattaaggg cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg
    22741 acaaaggcaa gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca
    22801 aaggccagac cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa
    22861 agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca
    22921 actccgggga aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct
    22981 acatggttgt aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata
    23041 tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag
    23101 cctccatttt ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg
    23161 acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca
    23221 ttgagcacct gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag
    23281 aaattgcccg cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg
    23341 gggttgaccc gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg
    23401 gcctgggcgt gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg
    23461 cttatatcgt cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag
    23521 acgaacacgt taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt
    23581 tgcgacgttt tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct
    23641 tagccaacaa ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc
    23701 gacgctggaa cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca
    23761 aatcgacact catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac
    23821 cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga
    23881 caccatttac accgctggta caccatccgg caccagcggt gactacattg acgacgagct
    23941 gccggtctac cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga
    24001 gctgacaccg ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga
    24061 tgaaaccggt tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga
    24121 gatcgacatc gacgaggttg aagccgtcct taagcggatc caacggtttg atccggtcgg
    24181 tgtagcggca aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac
    24241 cacgccgtgg ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa
    24301 tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc
    24361 cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc
    24421 tgagtatgtc attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact
    24481 caacagtgac agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa
    24541 cgcgcgcaac gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt
    24601 gatcaagagt ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga
    24661 acagcagcaa gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga
    24721 tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata
    24781 cctgcatagt ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac
    24841 cgagggcggc ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc
    24901 ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca
    24961 aggtatcatg gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc
    25021 gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca
    25081 acattaccgg aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat
    25141 ttgccaaact tgagcaatat tttgaccgaa tcaaccaagt ctatgttgtt ctgaaagtgg
    25201 agaaagtcac ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca
    25261 gcgcggaagg tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc
    25321 agttgaccaa acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg
    25381 catggcggcc tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt
    25441 ccactgtttg agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac
    25501 agcttagcag tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa
    25561 aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg
    25621 tggtttttga agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta
    25681 ttgccattcc gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc
    25741 agctcgaaac gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg
    25801 ccctgctggt gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga
    25861 aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc
    25921 tgtatcaaat cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg
    25981 tctcttttag acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt
    26041 caggttcagg taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg
    26101 ataaccttcc cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt
    26161 ctgccgccgt cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg
    26221 cgatgagtaa cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta
    26281 ataccttaat tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc
    26341 tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag
    26401 cggatctgat tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta
    26461 cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca
    26521 aacacggtat ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc
    26581 actgggatcc gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg
    26641 accgccacac agaagtacac aattttatct accagacgcg aagctatctt gagctatggt
    26701 tacctatgct ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg
    26761 ggaagcaccg ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta
    26821 aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac
    26881 tgttgaaatc acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt
    26941 aatgcagggt tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc
    27001 caacagcgtt attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga
    27061 agcgaccggt ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg
    27121 ttttgatgaa gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca
    27181 ataagaaaat cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct
    27241 tttcactttc agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag
    27301 ttaatcgatc atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta
    27361 tctgaacaaa gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat
    27421 gcccaacgct gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc
    27481 cgtgagttaa agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg
    27541 cgctatcacg gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc
    27601 gattttgacg gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa
    27661 gaagacattg cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac
    27721 cgtatcgtga cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca
    27781 gaggttatcc cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc
    27841 tggtcgaagt ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa
    27901 ccggcgctgg ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc
    27961 ctcacttcgc gcgaagctta atccagctgg tgttgttgca taaacggctc actgcctaac
    28021 tgatacatct gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag
    28081 actttgaaac gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta
    28141 agtttgctcg cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg
    28201 tcgccaaatt cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt
    28261 tctatcccca gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat
    28321 aaaaagagat ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca
    28381 ttgcgctcgt tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca
    28441 ggaaatttct gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc
    28501 atactgaccc agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc
    28561 tgtcgctcga ccattaccgc tgagaaggga acaggcgcaa cgctaaacaa cgcgatgccc
    28621 ccgccccaga aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta
    28681 aacaccgtta agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat
    28741 caatgccgct cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa
    28801 tcttattgtc ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca
    28861 tctcttccag cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat
    28921 cgaaaatttt cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct
    28981 gatgcatcgc ttgtgccagc tcctttaatt cacggtcaac ggtgcattcg ctaccaagac
    29041 tggcaaaatt gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca
    29101 gttcagcggc atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt
    29161 caatcagcac attgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa
    29221 cctgctgctt atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca
    29281 acgtcaacac cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta
    29341 cgccaatttt cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt
    29401 aataaaaatg gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg
    29461 ctatcttaac tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa
    29521 tttacggcgc agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc
    29581 cggggtcatt ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc
    29641 cactcttctt tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca
    29701 ggagactgaa tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca
    29761 cctttaattt tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac
    29821 tccagcacgc tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc
    29881 agcttcggtc ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct
    29941 gatttactgt tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat
    30001 ttcttgatca tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt
    30061 ccagcattga ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc
    30121 ggtaaatcct cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata
    30181 tctggcaact gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc
    30241 gccgccttgc cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc
    30301 gcgacaatca cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc
    30361 atatcgtctt catcaaacgc atcatcgacc tcttctgcca ccgacggtgc gtggatcgtc
    30421 aacgtaaagg ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc acccatattt
    30481 ttcgccagac gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca
    30541 tgactgtctt tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg
    30601 ccgataccag aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc
    30661 acggtaacct ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc
    30721 tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt aggctccagg
    30781 ttaaagcgca atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg
    30841 aagctggtga aatcaaccgg ctgattatca agctggacct tgcgccgttc catcttatcc
    30901 atgtcgataa tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatagttttg
    30961 agatattttt cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg
    31021 ataccgttca gcggtgtacg caattcgtga ctgatggtgg aaataaacgt cgttttgtca
    31081 cggctggcgc gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag
    31141 cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag
    31201 caggctttgc gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga
    31261 cggaacactt tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca
    31321 gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg
    31381 ttacagccgg aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca
    31441 aggaaggaac gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct
    31501 ttgatttcaa ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga
    31561 acggcaattt cctgatttag ctgggcaata ttatttttta actgaacgtt gaggctcaaa
    31621 tcgcgctcgc gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc
    31681 tccagttgct cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca
    31741 aagaagatag aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc
    31801 gccatttgta ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc
    31861 accagaccta acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc
    31921 attagggaat tccttcacga caacctgccc caataatacc caattctgac agttacgtta
    31981 taaattgtgt gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg
    32041 gacgtcccag cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca
    32101 ttcccgtcca tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg
    32161 cggaaatgcg gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga
    32221 gcgtataatc ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa
    32281 tgctgccttt cacaatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca
    32341 ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt
    32401 gggaacacac cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa
    32461 aatcatggcc gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct
    32521 ccagccacac ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg
    32581 gcacgcagtc cgggcggata ccaacacaca aaccgacaat attggcctgg ctcaccgcct
    32641 gctgatacat agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt
    32701 aggccagata gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa
    32761 tggaacgatg ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac
    32821 cacgcccgat ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat
    32881 gcaccttttg cccataacga cgggtgagat caccaccaaa catattgact aatttctgta
    32941 actgcataat ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca
    33001 attcggcgat gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca
    33061 aaattaccga aatttcatgc ataatcacat aaatcacttt tgcttatctt gtgtcagatt
    33121 tttttatctc cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa
    33181 ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt
    33241 tatgacagtc aggaattgac tgtttctcta acgactctcc ttttagcctt aaagataaaa
    33301 tctattttaa tttcagctat ttaataaaga attttgcgct aaagcacatt tctgtaccaa
    33361 taagcttgcc atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt
    33421 tctggatgag cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta
    33481 ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa
    33541 ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag
    33601 agcctagggg aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt
    33661 ttcggcctga tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata
    33721 cacgcactgg cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac
    33781 ggttgcggct tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc
    33841 ggctggcgtt tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa
    33901 ctcgccgctg ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt
    33961 gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct
    34021 ctgccacgca ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc acgcgatatg
    34081 gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac
    34141 ttctacgtct gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg
    34201 gatctgccgc gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catttgcctg
    34261 ttccaccagc gcttctccac taacaccgta ccgcgctggc cgctggcgca accgttccgc
    34321 tatctggcgc ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg
    34381 cgtacctata aattccagac gccgctgatc cctgacctgc acgacgccgc gccattcgtc
    34441 aacgaaaccg gttctgactc cagttccatg gataacatgc tggaactgct gctggcaggc
    34501 gggatggata tcatccgcgc catgcgcctg ttagtgccac ccgcctggca gaacaacccg
    34561 gatatggacc cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg
    34621 gatggtccgg cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt
    34681 aacggtctgc gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct
    34741 gaagtcggta tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcggacca
    34801 ggcgaactga tggtgatcga cactcgcagt gggcgtattc tgcactcggc agaaaccgat
    34861 gacgatctga aaagccgcca cccttataaa gagtggatgg agaaaaacgt ccgccgactg
    34921 gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg
    34981 cttgccagct accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc
    35041 gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc
    35101 gtgctctcca gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg
    35161 actaacccgc caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc
    35221 ggtcgtgaaa tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa
    35281 tcgccgattc tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac
    35341 cgcgcagata cgctggatat gacctttgac gtcactaaaa ccacgctcga agcgacagtc
    35401 aaagagctgt gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgtt gctggtgctc
    35461 tccgaccgga atatcgctaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg
    35521 atccagaccc gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc
    35581 gccagcgccc gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt
    35641 tatccgtacc ttgcctatga aacgctgggc cgcctggtag acacccacgc gattgccaaa
    35701 gattatcgta ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc
    35761 atgtccaaaa tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg
    35821 gtcggtctac acgatgatgt agtgggcctg tgcttccagg gcgcggtcag ccgcattggt
    35881 ggggcaagct ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg
    35941 gcgcgcaagc cgattagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac
    36001 gcctacaacc cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc
    36061 gactatcagg aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg
    36121 ctggcaatta cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa
    36181 ctgtttaaac gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac
    36241 gaggcgctgg cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc
    36301 gaagacccgg cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt
    36361 cgcttcggcg ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc
    36421 cagggcgcga agccgggcga aggcggtcag ttgccgggcg acaaagtcac tccttacatc
    36481 gccaaactgc gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat
    36541 atctactcta tcgaagactt agcgcagctt attttcgacc tcaagcaggt taacccgaaa
    36601 gcgatgatct ccgttaagct ggtgtccgaa ccaggcgttg gcaccatcgc gactggcgtg
    36661 gcgaaagctt atgcggactt gatcaccatc gcgggctatg acggcggcac cggcgcaagc
    36721 ccgctgtcgt cagtgaaata tgcaggctgc ccgtgggaac tcggtctggt ggaaacccaa
    36781 caggcgctgg ttgccaacgg cttgcgtcat aaaattcgtt tgcaggttga tggcggcctg
    36841 aaaacgggtg tcgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc
    36901 actggcccaa tggtggcgct cggctgtaaa tatctgcgta tttgccacct gaacaactgc
    36961 gccacgggtg tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg
    37021 ttcaaggtga ctaattactt tgagtttatc gcccgtgaaa cccgcgagct gatggcacag
    37081 ctgggagtaa cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac
    37141 ggtttcaccg ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagcca
    37201 catgcaggta agtcactcta ctgcactgaa aacaacccgc cgtttgataa cggcctactg
    37261 aacgcgcagt tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc
    37321 tggttcgata tccgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc
    37381 cagacgcacg gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc
    37441 gcaggccaga gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat
    37501 gccaacgact atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt
    37561 ggttccgcct tccgcagcca tgaagcaagc attatcggca acacctgcct gtatggcgcg
    37621 accggtggtc gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc
    37681 ggtgctatca ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt
    37741 attgtctgta ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac cggcggcttc
    37801 gcttacgttc tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actagtcgaa
    37861 gtcttaagcg tcgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag
    37921 catgttcagc ataccggttc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc
    37981 gccacgaaat tcgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac
    38041 cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt
    38101 cagaatgttt atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag
    38161 atccgtaaaa ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg
    38221 caggctgacc gctgtctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac
    38281 aactacatcc caaactggct gaagctcgcc aacgaggggc gtatttttga agcggcagaa
    38341 ctgtcgcatc agaccaacac actgccggaa gtatgcggac gtgtctgccc gcaagaccgt
    38401 ctgtgcgaag gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt
    38461 gagcgttata tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg
    38521 aagcaaaccg gtaaaaaagt ggcgattatc ggtgcaggcc cggcaggtct ggcgtgtgcg
    38581 gatgtcctga cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc
    38641 gggctgctga ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc
    38701 cgtgaaatct tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac
    38761 gtacagctgg acgatctgct gagtgattac gatgccgtgt tccttggcgt tgggacttat
    38821 cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg
    38881 ttccttatcg ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc
    38941 gtcagcatgg aaggcaaacg cgtggtagta cttggcggtg gcgacactgc gatggactgc
    39001 gtgcgcacgt ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa
    39061 gagaacatgc cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc
    39121 aaattcaacg tccagccact ggggattgaa gtgaacggta acggcaaagt cagcggcgta
    39181 aaaatggtac gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc
    39241 gttgcaggtt ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt
    39301 ccacacaaca tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc
    39361 atcgctccgg aaggcaacga caacgctttc cagaccagca acccgaaaat ctttgctggc
    39421 ggcgatatcg tccgtggttc cgatctggtc gttaccgcca tcgccgaagg tcgcaaagcg
    39481 gcagacggca ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga
    39541 aaagagtcgg cgctgaaatt ctcctttctt tctggaaatc gcctcgcata atctgaagaa
    39601 aaccaatgta atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc
    39661 cattattcaa cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc
    39721 tcaacaggct accagcaaat ccacgacggc attattcatc tggtcgatag cgcccgaacg
    39781 gaaacggtac gtagcgttaa cgcgctgatg accgcgacgt actgggaaat tggccgacgg
    39841 attgtcgaat ttgaacaagg tggtgaggcc agagcagctt atggtgcaca gttgattgat
    39901 cggctatcga aggatttaag tcgacggtat aaacgtgggg tctctgcaaa aaacttacgc
    39961 caaatgaggc ttttttacct cttttttcaa catgttgaaa ttcgccagac agtgtctggc
    40021 gaattaacac cattgggaat tccgcagaca ccatctgcag aattcccatc ggcaaagatt
    40081 tggcagacgc tgtctgccaa attgagaggc tgtttgatat ttccacagct tttgtattgc
    40141 atctcctatt ttcggtttgt atattcatgc aaataaacga agg
//
LOCUS       DADKXM010000020        39911 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.020, whole genome shotgun sequence.
ACCESSION   DADKXM010000020 DADKXM010000000
VERSION     DADKXM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.020"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1334
                     /locus_tag="JQR05_001079"
     CDS             <1..1334
                     /locus_tag="JQR05_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY5323293.1"
                     /translation="LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKY
                     FHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANH
                     ANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDA
                     VNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTD
                     GVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASV
                     NATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDA
                     VNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASM
                     ASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1702..3357
                     /gene="lldP"
                     /locus_tag="JQR05_001080"
     CDS             1702..3357
                     /gene="lldP"
                     /locus_tag="JQR05_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY5323294.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            3357..4133
                     /gene="lldR"
                     /locus_tag="JQR05_001081"
     CDS             3357..4133
                     /gene="lldR"
                     /locus_tag="JQR05_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY5323295.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            4130..5320
                     /gene="lldD"
                     /locus_tag="JQR05_001082"
     CDS             4130..5320
                     /gene="lldD"
                     /locus_tag="JQR05_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY5323296.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            5516..5989
                     /gene="trmL"
                     /locus_tag="JQR05_001083"
     CDS             5516..5989
                     /gene="trmL"
                     /locus_tag="JQR05_001083"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY5323297.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(6042..6863)
                     /gene="cysE"
                     /locus_tag="JQR05_001084"
     CDS             complement(6042..6863)
                     /gene="cysE"
                     /locus_tag="JQR05_001084"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY5323298.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(6943..7962)
                     /gene="gpsA"
                     /locus_tag="JQR05_001085"
     CDS             complement(6943..7962)
                     /gene="gpsA"
                     /locus_tag="JQR05_001085"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY5323299.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(7962..8429)
                     /gene="secB"
                     /locus_tag="JQR05_001086"
     CDS             complement(7962..8429)
                     /gene="secB"
                     /locus_tag="JQR05_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY5323300.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(8492..8743)
                     /gene="grxC"
                     /locus_tag="JQR05_001087"
     CDS             complement(8492..8743)
                     /gene="grxC"
                     /locus_tag="JQR05_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY5323301.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(8885..9316)
                     /locus_tag="JQR05_001088"
     CDS             complement(8885..9316)
                     /locus_tag="JQR05_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY5323302.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            9561..11105
                     /gene="gpmM"
                     /locus_tag="JQR05_001089"
                     /gene_synonym="pgmI"
     CDS             9561..11105
                     /gene="gpmM"
                     /locus_tag="JQR05_001089"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY5323303.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            11115..12398
                     /gene="envC"
                     /locus_tag="JQR05_001090"
     CDS             11115..12398
                     /gene="envC"
                     /locus_tag="JQR05_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY5323304.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNDRKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            12402..13361
                     /locus_tag="JQR05_001091"
     CDS             12402..13361
                     /locus_tag="JQR05_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY5323305.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(13348..14382)
                     /gene="waaH"
                     /locus_tag="JQR05_001092"
                     /pseudo
     CDS             complement(13348..14382)
                     /gene="waaH"
                     /locus_tag="JQR05_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(14621..15646)
                     /gene="tdh"
                     /locus_tag="JQR05_001093"
     CDS             complement(14621..15646)
                     /gene="tdh"
                     /locus_tag="JQR05_001093"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY5323306.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(15656..16852)
                     /gene="kbl"
                     /locus_tag="JQR05_001094"
     CDS             complement(15656..16852)
                     /gene="kbl"
                     /locus_tag="JQR05_001094"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY5323307.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(17127..17984)
                     /gene="yibB"
                     /locus_tag="JQR05_001095"
     CDS             complement(17127..17984)
                     /gene="yibB"
                     /locus_tag="JQR05_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY5323308.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            18287..19219
                     /gene="rfaD"
                     /locus_tag="JQR05_001096"
     CDS             18287..19219
                     /gene="rfaD"
                     /locus_tag="JQR05_001096"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY5323309.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19229..20275
                     /gene="rfaF"
                     /locus_tag="JQR05_001097"
     CDS             19229..20275
                     /gene="rfaF"
                     /locus_tag="JQR05_001097"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY5323310.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKETWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20279..21271
                     /gene="rfaC"
                     /locus_tag="JQR05_001098"
     CDS             20279..21271
                     /gene="rfaC"
                     /locus_tag="JQR05_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY5323311.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGVAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPRLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            21268..22476
                     /gene="rfaL"
                     /locus_tag="JQR05_001099"
     CDS             21268..22476
                     /gene="rfaL"
                     /locus_tag="JQR05_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY5323312.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(22512..23654)
                     /locus_tag="JQR05_001100"
     CDS             complement(22512..23654)
                     /locus_tag="JQR05_001100"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY5323313.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(23663..24676)
                     /locus_tag="JQR05_001101"
     CDS             complement(23663..24676)
                     /locus_tag="JQR05_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY5323314.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDEAIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(24701..25408)
                     /gene="rfaY"
                     /locus_tag="JQR05_001102"
     CDS             complement(24701..25408)
                     /gene="rfaY"
                     /locus_tag="JQR05_001102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY5323315.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAAKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(25434..26441)
                     /gene="waaO"
                     /locus_tag="JQR05_001103"
                     /gene_synonym="rfaI"
     CDS             complement(25434..26441)
                     /gene="waaO"
                     /locus_tag="JQR05_001103"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY5323316.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(26484..27281)
                     /gene="rfaP"
                     /locus_tag="JQR05_001104"
     CDS             complement(26484..27281)
                     /gene="rfaP"
                     /locus_tag="JQR05_001104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY5323317.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(27274..28398)
                     /locus_tag="JQR05_001105"
     CDS             complement(27274..28398)
                     /locus_tag="JQR05_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY5323318.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQSKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(28395..29453)
                     /gene="rfaQ"
                     /locus_tag="JQR05_001106"
     CDS             complement(28395..29453)
                     /gene="rfaQ"
                     /locus_tag="JQR05_001106"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY5323319.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYLDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29866..31143
                     /gene="waaA"
                     /locus_tag="JQR05_001107"
     CDS             29866..31143
                     /gene="waaA"
                     /locus_tag="JQR05_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY5323320.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31151..31630
                     /gene="coaD"
                     /locus_tag="JQR05_001108"
     CDS             31151..31630
                     /gene="coaD"
                     /locus_tag="JQR05_001108"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY5323321.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(31669..32478)
                     /gene="mutM"
                     /locus_tag="JQR05_001109"
     CDS             complement(31669..32478)
                     /gene="mutM"
                     /locus_tag="JQR05_001109"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY5323322.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(32576..32743)
                     /gene="rpmG"
                     /locus_tag="JQR05_001110"
     CDS             complement(32576..32743)
                     /gene="rpmG"
                     /locus_tag="JQR05_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY5323323.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(32764..33000)
                     /gene="rpmB"
                     /locus_tag="JQR05_001111"
     CDS             complement(32764..33000)
                     /gene="rpmB"
                     /locus_tag="JQR05_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY5323324.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(33217..33885)
                     /locus_tag="JQR05_001112"
     CDS             complement(33217..33885)
                     /locus_tag="JQR05_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY5323325.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            34057..35277
                     /gene="coaBC"
                     /locus_tag="JQR05_001113"
     CDS             34057..35277
                     /gene="coaBC"
                     /locus_tag="JQR05_001113"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY5323326.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            35255..35713
                     /gene="dut"
                     /locus_tag="JQR05_001114"
     CDS             35255..35713
                     /gene="dut"
                     /locus_tag="JQR05_001114"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY5323327.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            35820..36416
                     /gene="slmA"
                     /locus_tag="JQR05_001115"
     CDS             35820..36416
                     /gene="slmA"
                     /locus_tag="JQR05_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY5323328.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(36453..37094)
                     /gene="pyrE"
                     /locus_tag="JQR05_001116"
     CDS             complement(36453..37094)
                     /gene="pyrE"
                     /locus_tag="JQR05_001116"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY5323329.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFSRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(37160..37876)
                     /gene="rph"
                     /locus_tag="JQR05_001117"
     CDS             complement(37160..37876)
                     /gene="rph"
                     /locus_tag="JQR05_001117"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY5323330.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            38003..38866
                     /locus_tag="JQR05_001118"
     CDS             38003..38866
                     /locus_tag="JQR05_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY5323331.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEIMQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            39087..39911
                     /gene="dinD"
                     /locus_tag="JQR05_001119"
     CDS             39087..39911
                     /gene="dinD"
                     /locus_tag="JQR05_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY5323332.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKELKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT        10294 a         9981 c         9493 g        10143 t
ORIGIN      
        1 cattgtcaat tggtgatgac ggtaaatacc gccaaatcat caacgtagct gatggttcag
       61 aagcccatga cgccgttacg gttcgccagt tgcagaacgc tattggagcg gtcgccacta
      121 cgccaaccaa gtactttcac gccaactcaa cggcagaaga ctcactggcc gttggtgaag
      181 actcgctggc aatgggtgcg aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt
      241 tgaacactct ggttctggct gatgcgatca acggcattgc tatcggcagt aacgcacgcg
      301 caaaccatgc aaacagtatc gcaatgggta acggttctca gaccacccgt ggtgcacaga
      361 ctggctacac cgcctacaac atggacgcac cgcagaactc tgtaggtgag ttttctgtcg
      421 gtagtgaaga cggtcaacgt cagatcacaa acgtcgcagc tggttcagcg gataccgatg
      481 cggttaacgt gggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
      541 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
      601 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
      661 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
      721 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg
      781 taggttcttc tactaaccaa cgccgtatca ccaacgtagc tgccagtgtt aatgccaccg
      841 atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg cggcgtgcgc tacgacacca
      901 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggtggcaac ggcagtacga
      961 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     1021 tgaagcaaag cgcgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     1081 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
     1141 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     1201 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     1261 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     1321 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     1381 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa gggcaattct
     1441 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     1501 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
     1561 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     1621 gcgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     1681 gtggactcta ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     1741 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     1801 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
     1861 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc
     1921 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
     1981 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     2041 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
     2101 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     2161 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
     2221 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
     2281 atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     2341 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc aggcggctcg
     2401 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     2461 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca accagttcgc
     2521 gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc ccacaccggt
     2581 tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac cgctaccgta
     2641 acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
     2701 tgggtgatca acattccggt gccgtacctc gataaactgg ttgcccgtat gccaccagtg
     2761 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     2821 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     2881 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
     2941 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
     3001 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     3061 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     3121 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     3181 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     3241 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     3301 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     3361 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     3421 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     3481 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
     3541 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     3601 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
     3661 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     3721 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     3781 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     3841 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     3901 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     3961 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
     4021 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     4081 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     4141 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     4201 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     4261 agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     4321 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
     4381 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
     4441 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     4501 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
     4561 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     4621 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
     4681 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     4741 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     4801 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
     4861 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     4921 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     4981 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
     5041 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     5101 caccgtactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
     5161 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     5221 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     5281 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc
     5341 taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     5401 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     5461 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     5521 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
     5581 cgctaatacc ggctttcgtc tacatatcat cgaaccgatg ggatttgcct gggacgataa
     5641 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
     5701 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
     5761 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
     5821 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
     5881 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
     5941 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
     6001 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
     6061 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
     6121 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
     6181 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
     6241 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
     6301 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
     6361 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
     6421 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
     6481 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
     6541 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
     6601 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
     6661 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
     6721 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
     6781 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
     6841 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
     6901 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
     6961 gtccttgcgt gcacgaccta ataatgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
     7021 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
     7081 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
     7141 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
     7201 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
     7261 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
     7321 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
     7381 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
     7441 gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg aggccagcga
     7501 aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
     7561 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
     7621 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
     7681 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacgactggc
     7741 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
     7801 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
     7861 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
     7921 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
     7981 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
     8041 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
     8101 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
     8161 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
     8221 tcgcccaagg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
     8281 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
     8341 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
     8401 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
     8461 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
     8521 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
     8581 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
     8641 cgatcggcaa ctcctggaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
     8701 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
     8761 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
     8821 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
     8881 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
     8941 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
     9001 gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa
     9061 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
     9121 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
     9181 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
     9241 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
     9301 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
     9361 ggagtatacc tgcgcggtgc ggcaaataca gcaagcgcgt taactggaat gcaattttgc
     9421 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
     9481 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
     9541 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
     9601 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
     9661 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
     9721 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
     9781 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
     9841 ttgctaatcc agtgctggct ggcgcggtac ataaagcgaa aaacgcaggt aaagcagtac
     9901 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
     9961 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    10021 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    10081 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    10141 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    10201 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    10261 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    10321 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    10381 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    10441 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    10501 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    10561 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    10621 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    10681 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    10741 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    10801 cggttaaagc agttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    10861 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    10921 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    10981 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    11041 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    11101 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    11161 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    11221 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    11281 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    11341 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    11401 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    11461 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    11521 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    11581 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    11641 aaacgcgtga agaagtcacc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    11701 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgatc
    11761 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    11821 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga
    11881 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgagcgc cagaaagaag
    11941 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    12001 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    12061 atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtt atcggtgcct
    12121 ctgaaggtac tgaagttaaa gcaattgccg acggtcgagt gattctggct gactggctgc
    12181 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    12241 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    12301 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    12361 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    12421 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    12481 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    12541 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    12601 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    12661 agccgctgga gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta
    12721 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca
    12781 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc
    12841 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    12901 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    12961 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    13021 ttggacatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    13081 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    13141 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    13201 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    13261 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    13321 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    13381 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    13441 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac cggaagtaaa
    13501 tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcgca ccgcatggca
    13561 aacacgcaat gcttcgtaag ttatttgctg atgaaattcc ggataaatcg taattttgtc
    13621 ggcataattt cgatttaatt tctccagcag gcgggtaatc ttaatatagt gacgttgata
    13681 attaaggttt ttattccctt gtctatgcaa ccgactcact gacgtattat gcagataata
    13741 tttatataat tattgctcgg tatatcgcgc acgcagcgcg ttaaacatga attctgttgt
    13801 ccagacaata tcctgatgat gtaatccggc aataaattta atgttatttt taacaataac
    13861 atcacggcga taaaccccca tccagacaac gtgagtccaa cggcgcgaag aaagccccat
    13921 ccgcagccag tccgggccgg ttaatacacc ggttgagcga aggcgatcgc tggggatgga
    13981 ttgccaagtt tctcccgttt cacgaaaaga ccagtcagcg ttgcactgcg ccacgtcgag
    14041 gtcgtcctct aacgccatgg tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc
    14101 agcatcgaca aaagcgacat atttgcccgt cgccacctcg atcccacgat tacgcgctac
    14161 cgatgtgcca gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg
    14221 ctttgctatt tcaacagaat tatccgttga accatcgtta ataataatga tttccagagc
    14281 agtccaggtt tgcgtaatta aagattccat acaagtgcgg aaatcatcgc ccgcattata
    14341 taacggaata ataacactaa gtttattggt gctgttcatc ataaattacc gacgacctct
    14401 gaagttatcc tgacgtttta caggctttgt attaagaaac tattaagcct gtgagatatt
    14461 atttttacag gtgagagagt cacttcaggg tagagagata atgagaacag ctatataaaa
    14521 gatcagcgca attacgaact ctttattaca ctgacaggat tttacataat taacagatta
    14581 tcctcagata aaagggctgg aataccagcc cttgttcgtg ttaatcccag ctcagaataa
    14641 ctttcccgga ctggcccgaa cgcatagcgt caaagccctt ctggaaatca tcgatagaga
    14701 aacgatgggt aatgatcggc gagagatcga ggccagactg aatcagcgcc gccatcttgt
    14761 accaggtttc aaacatctca cgaccgtaaa tgcctttaat gaacaagcct ttaaagatca
    14821 ctttggtcca gtcgatagac atatcagacg gtggaatacc cagcatcgca atacgaccgc
    14881 cgtggttcat ggtatcgagc atggtacgaa acgctggcgg cgcgccggac atttccagac
    14941 cgacatcaaa gccttcggtc atgcccagtt cagccatcac atcattaagg ttttctttcg
    15001 cgacgttaac cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg tattcgttaa
    15061 catcggtgat caccacattg cgtgcaccaa cgtgtttcgc caccgccgca gccataatac
    15121 ctatcggacc tgcaccagaa accagcacat cttcgcccac cagatcgaac gacagcgcgg
    15181 tatgcacggc gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag atattgtcgg
    15241 ggattttgaa agcgttgaac gccggaatca ccagatattc ggcgaagcag cccggacggt
    15301 taacaccgac accaatggtg ttgcggcaca gatgggtacg accgccacga cagttgcgac
    15361 agtgaccgca ggtgatatgg ccttcgccag aaacgcgatc gccgattttg aagcctttca
    15421 cttcctgacc aatacctacc acttcaccga catattcatg gcccacgacc atcggcaccg
    15481 ggatggtttt ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg
    15541 ctgttttacg gattttaatc agcagatcgt tatgcccgag ttccggtaca gggacgtcgg
    15601 tcatccagat gccctcttcc gctttcagtt tggataacgc tttcatctca catcctcagg
    15661 cgataacgcc cagttgttta ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct
    15721 caggggtatg cgccgcagac atctgggtac gaatacgcgc ctgacctttc ggaacgaccg
    15781 gatagaagaa accggtgacg taaatgccct ctttttgcag ctctcgggca aatttctgcg
    15841 ccactaccgc atcaccaagc atgaccggaa taatggcgtg atcggctccc gccagggtaa
    15901 agcccgccgc cgacatttgt tcacggaact gacgcgcgtt cgcccacaga cggtcacgca
    15961 actcgctacc cgcttcaacc atctccagca ctttgatgga agcggcgaca atcgctggtg
    16021 ccagcgagtt ggagaacagg tacggacgag aacgctggcg cagccactca accacttctt
    16081 tgcgcgccgc ggtataacca ccagaagccc cgcccagcgc tttaccgagc gtgccggtga
    16141 tgatgtcgac acgccccatc acatcgcagt attcatggga accacgacca ttttcaccga
    16201 caaaaccgac cgcgtgggag tcgtctacca tcaccagggc atcgtattta tctgccagat
    16261 cgcaaacgcc cttcagattg gcaatcacgc cgtccatcga gaacacacca tcggtggcaa
    16321 tcagcacatg acgcgcaccg gcttcacgcg cttctttcag acgagcttca agctcctgca
    16381 tatcgttgtt ggcatagcga tagcgtttag ctttacacag acgcacacca tcaataatag
    16441 acgcgtggtt cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc gtttcaaaca
    16501 ggccaccgtt agcatcaaag caggaagagt agagaatcgc atcttccatc cccaggaatg
    16561 ccgccagttt ttgttcaagc tctttatggc tgtcctgagt gccgcaaata aaacgcaccg
    16621 aagccatgcc gaaaccgtga gaatccattc ccgcctttgc tgccgcaatc agatccggat
    16681 gattcgccag cccgagatag ttgttggcac aaaagttaat gacgtggctt ccatcagcca
    16741 cggtgatatc tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac aacccttccg
    16801 cccgtgcggt ttccagatcg ttggttaact gctgataaaa ttctccacgc attgcgattc
    16861 tccagacagg gcaaattcca gcacatatta cccaaactta taggtcgcga cgagatgatg
    16921 cgttaacact tctgcaaaat tcaggataaa taacgtgtat cttgatgtct ctcaacacct
    16981 acagatagta gaaaaactgt acagttttgg ctgagcataa tgatgagtga ttttactgca
    17041 ttgatataaa aagaaaaaat gatgccaaag catcattttt cctatctcag ttggcatcaa
    17101 aacgctaccc actaatggag ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc
    17161 atttttgccc ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca ttttcctctc
    17221 cccagatagt ttaggttgaa gagatcggaa cgtttgtaat agcacatgac aaaaatgccc
    17281 tggtcatcgt ccacaatgtt attgtttagt gttatttttt gactttccag aactaattta
    17341 tagaattctt tccatttatg ctgtgaacca acaattgcgc ccccaataat atatacatga
    17401 ttgccaatca tgaaatcaaa agcctgttgt tgagatgtaa cagtaagccc tttttttata
    17461 gtgaaaagat gcatcttatt ttcatcaaat gggaaatccc aaattttcaa tcctcgagtt
    17521 acatttggct tacggcaata gccaaaatct atccacgcaa caagaggcgt tttcactaaa
    17581 cccatgttga tagctttatt tacaaaatag gccttaaggt tacataccaa aacatactct
    17641 ggtgaccagt actctgggtt ttttaattgt cgaggctcaa gtctatttgt aaacgactca
    17701 tctttttgaa ttttttcgat acgacttctg atatatctaa atttcttttt tatatcgata
    17761 acaataaccg ttgttggttt tccgttgcga atcgcttcaa tccttggttt caaatcggga
    17821 gaagtgaaaa taatcatctc attttcaagt gcggccagac gctcaaagta actaaaataa
    17881 acatcaactg aacgagctag tttttcacgg aaccctttat tagcagtcca atcccctcta
    17941 ccaatgtcaa aatatgcagt gataattgtg gtggatgatt tcattactaa taagaccatt
    18001 aatataaaaa agaattatat cataaatatt aacagaatgt atactgtata ttatccattc
    18061 aaatgattcc gttatttatc atacatcgga atattgatac taaagcacta ttcacatgca
    18121 aaaccaacat ccgccatgaa ggactagcta aaacccaaac tagtttgttg caattagcat
    18181 ccttgcacct ctatgtaaag ggctgaaggg attcggatgt gatggtatga ttacagacat
    18241 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    18301 cggcggcgcg ggctttatcg gcagcaacat cgttaaggcc ctgaatgata aaggcatcac
    18361 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    18421 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    18481 gttcggtgat gtcgaagcga ttttccacga aggcgcatgc tcttccacca ccgagtggga
    18541 tggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    18601 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acctacggcg gacgcacctc
    18661 cgactttatt gaatcccgcg agtacgaaaa accgttgaat gtctacggtt actcaaaatt
    18721 cctgtttgat gaatatgttc gtcaaatcct gccagaagcg aactcgcaga ttgttggctt
    18781 ccgctatttc aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc
    18841 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    18901 gaacttcaaa cgtgacttcg tctatgtggg cgacgtggca gatgtaaacc tgtggttcct
    18961 ggaaaatggc gtttccggta tcttcaacct cggcaccggt cgtgcagaat ccttccaggc
    19021 agtagcagat gctacgctgg cttatcacaa gaaaggccag atcgaataca ttccgttccc
    19081 ggataagctg aaaggccgct accaggcgtt cactcaggca gatctgacaa atctgcgcgc
    19141 ggcgggttac gacaaaccgt tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg
    19201 gctgaatcgc gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    19261 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    19321 gataatcgat gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    19381 taacgaagct atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    19441 actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac caaactcctt
    19501 caaatctgca ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga
    19561 gatgcgctac ggtttactca acgatgtacg cgtgctcgat aaagaaacct ggccgctaat
    19621 ggtggaacgc tatgtcgcgc tggcctatga caaaggcatt atgcgtaccg cacaagatct
    19681 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    19741 taatcaattt tcgctttcat cagaacgtcc gatgattggc ttttgcccgg gtgcggagtt
    19801 tggtccggca aaacgctggc cacactacca ctatgcggaa ctagcaaagc agctgattga
    19861 tgaaggttat caggtggttc tgtttggctc tgcgaaagat catgaagcgg gcaatgagat
    19921 tcttgccgct ttgaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca
    19981 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgtcacta acgattctgg
    20041 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccaagtag
    20101 cccggacttc acaccgccgc tatcccataa agcgcgcgtg atccgtctga ttaccggcta
    20161 tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac
    20221 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    20281 gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact
    20341 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaaggggt
    20401 cgcacagatt ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg
    20461 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    20521 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc
    20581 actggtgacg cgtctggcgc atggcgtaaa gcatggattg gactggcaaa ccgctcgcga
    20641 acctttagcc agcctgtttt acaattgtaa gcatcatatt gcaaaacagc agcacgccgt
    20701 agaacgcacc cgcgaactgt ttgccaaaag tttgggctat agcaaaccgc aaacccaggg
    20761 cgattatgct atcgcacagc attttctgac gaacctgcct acagatgctg gcgaatatgc
    20821 cgtatttctt catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga
    20881 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    20941 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    21001 gatgagtctg gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga
    21061 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    21121 aaccgatccg agattaattg gtgggtatgg gaagaatcaa gtagagtgtc gttctacaag
    21181 tatgtctctt gcagatttgc cagctcaaac cgtttttcaa aatttaaatc ttgaaataat
    21241 aaccaataag ttgacatcgg agataagatg acctcaacat tatttttctc tctcgagaaa
    21301 aaaaactgga tagcgtactg gaacagagct ctcgtattct tattcattac cacctatttt
    21361 ttgggtggga ttacaaggta taaacatctt attgttattc ttatgacaat aacgacaatc
    21421 gtctatctct gcaaacggcc aaaacactat ctctcactat ttaaaacatt tctttttggt
    21481 agtgttgcca tattaactat tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt
    21541 gctagcatga aggaaatttt caaagctatt attgagaata ctttactatg cacaatagca
    21601 ataccggtca tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca
    21661 tttattagtg cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat
    21721 caacaaggga taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc
    21781 tttttattcc ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct
    21841 tttgtggttc taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc
    21901 tggctttccg tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta
    21961 ctattagtag gagtaatggt ttccatcatt gcattgtcgg ttattttcac acataaggag
    22021 atgaccgcaa agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat
    22081 ggtactcaag gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac
    22141 ggtaacgttg catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc
    22201 tttagacaat caatagggcc acataatttt gcgctattca tctggtttgg cactggttta
    22261 ttagggctgg taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat
    22321 ggcgtcaaga ataaatatcg ctcaccatat aatgcatatt atataatctt actatctttt
    22381 ataggttatt ttgttatccg tggaaacgta gaacaaattg aaccaaattt attaggcgtt
    22441 tacgccggct tattattagc gatgaaaaac aagtaaaaaa taaaaaaggc tgcataatgc
    22501 ggccttttta tttattcaaa ccaattatga ataacctctt cgaatctttg agttacgcca
    22561 tcccaactgt agtgatcaaa cacaaagtcc tgaccttgct ttgcaactgc tgttaattca
    22621 ggattcgcta acgtttttaa aatatcgctg cttatggagt ctgcagtcat aggttctttt
    22681 aaatggaatc ccgtagtatt ttctttgaca aattccgtca tccccccacg cgtactcact
    22741 aaaacaggct taccagcacc catagcttca attgccacca tgcaaaatgg ttcctggaac
    22801 tgggaaggaa tgacgactaa atctgccagt gggtaataac aatgcatctt ttcaggcggt
    22861 atgcttccta acataatgca acgatccttc aaacgttttg caatttccct gacattgcgt
    22921 tgatatgcac ctttgtcact tttactcatt tctgtataat caccaatcac aactaatttt
    22981 aaatttttgt gagcagcggc caatttttcg aatgattgca tcaacagcaa tatgcctttg
    23041 tcagggacga tacgcccagc ataaaaaata attttctctt caggggagat attaagttcg
    23101 cttctagtga tcggctgaaa attagattga taggtttcta aatcaattcc atttggtaca
    23161 atctcaatat ctgcatttgc taaataagat tggtaatact ttttcaagta taaactaggg
    23221 actatcattt taacattttg ctcaagcaat tttggttcaa atgcattatg catatgtata
    23281 accactcttg cctgaggagc tcgcttacga atttgagtgt atagcttcat gctattatga
    23341 actacaataa cactttcttt cgttatagga aaatcatgtg caatattcaa tattctctgt
    23401 gaatatggaa gaggatcaag tcttgtccat ttttgaaata accgcttata tatgcgactg
    23461 aatccaaccc tatgaatact acaattatca ttaacaaagg tgtaatttga gtatcctgga
    23521 tttttgatac aaacaatacg atttggaaaa ttggtacgct gagcaacctg atacatccac
    23581 gtttcaacag cagccgctcc gcgaggagga attgaaaaaa taggagtaac cgtaaatatt
    23641 attttatcaa ccatctcgta tattaacctt tcataacatt atatttaatt gctgtaaaaa
    23701 caccatcgag aaattttttc tggtaaagca agtgtttata tttttctttg tgctcatgtt
    23761 ttttaacagc ttttttataa ggtatatttc tccatggtga tatattataa atattacgaa
    23821 aataatctgc agaggcgtag ttcgcccaat catgccatgg cttagttata ccagtataat
    23881 gtatgaagac agtgtcatca ttaataaacc gggtgtaata ttcactattt ttttcttcaa
    23941 attctgactt tatagtataa atacaattat attttctcgg taatatttta gcatgatgta
    24001 gaaacattaa gtttaaaata tcttgatccg ggtactttaa tttttttata attgcctcat
    24061 ctgataatag atcaaagaat ttttccgtta gtctttgttt taaccattcc cgtaaattaa
    24121 tatacattac accagagtta aaatagctac cgttaaaatc ctcattacac aaacgctcag
    24181 cgcttttact ttgcatagca tctacatcaa ttaccactgc cccatattca tctttaaact
    24241 ctaatgctat taactcgttc aatgaaccct tacagacgat atcagcatca agatataata
    24301 atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa acgataataa atagatactg
    24361 gccagatatt tgattgaggt agctccttca atggctcaga atcaatatga tataatttaa
    24421 ttactgtttg atacttcgtt gctaattgtg ataataattc aatatactct ttgtctaagt
    24481 aatcagcgat tatgtgaaag gtaaatttgt tttgtttatt attttcaaga attgaagcaa
    24541 ttgaaatcgc agccccaacc tggtagttct catcaatacc ccatgagaca ttaaacgttt
    24601 cccgttcatc aagtttaacg gggcgcttgt ccagttcaat tatatcttta aattgagtaa
    24661 gatgtttaaa atccaattta attacccttc atcataatca ttatttgctc ttcaatccta
    24721 gtttgacttt tacatccctg attacttttt taattttctt tttgaatata agatatgtat
    24781 agccaaagtc tttcaattca ttttttatat tatagtgccg ttcaagatca atgcgatctt
    24841 ttgcttttaa aacagcggtt gtttttttac ctgaaaggtc aattagtctt aaaccttttt
    24901 cggatactat aaaatttcct ttatgaggat cgccagacac cattccatgc tgatgcaact
    24961 cttttatcga ttctgataat tgatccttca gatcttcaga aatttccaaa tactcattta
    25021 acccgacacc ttcaatatac tcaatgagca taatataata atgagcgaaa tttaatgttt
    25081 tacgttcagc tagcaaaaaa tagtcattaa ttgattggat gccttcacca cgtacccgat
    25141 cggtttgata aattaaattt tcataataat ctttttttat gcaagatttt aagaaacgtt
    25201 cggtcatttt atgctttgcc gcataaactt taagtacaag aggtccacga gcagtgtcaa
    25261 taagaatgac tttagtatca tcaatagaac gaaaaacttt taatgtttta accctgcaag
    25321 ttaaaaagtc atttagaact tgctcataaa agggatcatt atcttttatg aaaactttca
    25381 acccatttat cgttttatta tatatcataa tgaattattt taaccttaaa tctttagaag
    25441 catttttctt tataatactt taaataatta aaaatgcctg ccatataatg cttttgttta
    25501 aatttatgtt ttgcacaata ccgatattga ttgctattta caggtttaag taaatcttct
    25561 ttactccacg gagaagctgc tttggcgatc aaaaaactac gtgaaaccgg ataatcagcc
    25621 cactcatgcc agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact
    25681 ggattatcaa ctttgtcttt taattcatag ttaatactat atcgggtatt atatttctcc
    25741 gaaataaact tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg
    25801 attttactta cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt
    25861 agattccact catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaac
    25921 tgtggtgtag ttaataccga ggctcgattt tgccaccatt caatatctct ttcagctaca
    25981 actgcggcaa tttcattagt agaaaattga taatctaaga gttctttaat actacccttg
    26041 caagcaatat ctgcatcaag atatagtatt ttttcatgtt tatgatagaa ataatctgca
    26101 attataaatc gaaaatatgt tgcgtaagtc cagtttttcg tgcttggtaa tgacttcagc
    26161 ttatcacaat tgataacata aatattaatt cttgaattat attgttttgc taaatcagaa
    26221 aaatataatt tgtctttatc actgatataa tctgtgaaaa catgaaattc acaagagatt
    26281 tctctattgt ttaatagaat agatgcgatg gctacaccac atccaaaaag aaaatttctg
    26341 tcaataccaa atgctatatc aagagtattt ttagatctta tattttgata atgaaaatta
    26401 tactcaaaaa ggatgatgtc actatcattg agttgagaca tatttattct gtatccaaaa
    26461 ttttggcaaa aataagttac gggttacaac gattttcgaa tcgttctttc cctgattttt
    26521 gtggcttttg cttctgcttg cgacagcagt ccttgttcct gcttgagaat gtctttaagc
    26581 ggggcggcaa aatacacctt cataaagcgc cagatatccc gctgagtcag gccaatattc
    26641 attgaagaaa aataaagccc tataagatct ttatcgcgcc aacgacgtgg aacgcgcgtg
    26701 cgaagctgcg cccggtgtag gtcaattacc gaaattttta actcctcttc cttaccggaa
    26761 aaaggcaagt gcagcaggaa atgacagata taacagtcac gatggttaat gcccgcagca
    26821 tgcatatcgc gcaccatcgt cgcgacacgc ttaataagca tacgctttac gcgaacatct
    26881 ggtgggttgg tcgcccagtc agcacagtaa tcttccagac tgatggttgg tgtcagatct
    26941 tcggtaataa taaacgaggt tctggtcagc ggattcatgc ctttttcgcc gaatgccacc
    27001 ccatacatag tatcaacgcc gacatcccgc agtcgatgaa tcgcattcca ttcgcggtct
    27061 gcgcctaata ctggcatccg caatgagagt aaatttttga ttatctcttt cagggtcgtg
    27121 ccgcgatgcc atttgagaaa atagcttttt cccgccattt caaagcgcag agtacggcga
    27181 gtttccagtt cacgaaatac ctcaccctgc aaggttttaa cttcctcaaa aggatctttg
    27241 ccgcgccata acgtggcaaa cggctcttta agttcaacca tccagaccac ccgttatgat
    27301 atccgccgct ttctctggca gactgtataa atcttgtgta tcagcataat gtcgcgcatt
    27361 ttccgcccaa gcctggcgca atgaagattg cgttaacgct ttgcgtaaaa tctcattcaa
    27421 tgtttcctgg cggaatggct cagcaatagc ctcgccgcaa ttagcgtcta caatataatg
    27481 cgcatagcca caaacggcag ttgttagtac cggtaatcct gcagtaatcg cttccagcag
    27541 cacaattccc gccgcttcct ggtaggcagg atgcagtaat aaatccgccg ccgccattaa
    27601 ttccgagaca tcgttgcgcc ccgagaagaa gtgaacatta ctgcgcacgc cgcgtttttc
    27661 tgccagtgcc tcaaattttc gcggtttatc ctgcccaaca acatatagca atgtgttgtg
    27721 gcgcagcgaa tccggtaacg aagcaagtgc ttcaatggaa cgatcgacac ctttacgcgt
    27781 gaagtctgaa ccgacctgca acaataaata ttgttgttcg gttattccat tcttcttacg
    27841 gaagatttca cggctattgg ctggctgctg gctatattta cgatcaggat aaatccccgg
    27901 tggcagaata tgaaaacgct ccgcttcagt ctgataatgt ttctggaaat cggcgatttg
    27961 cttatctgtc agcatcagca gctgtgtcgg cttgctctgt tcgaaggttg cccgctcaaa
    28021 ggcggcataa tggcgataac gtgacgtcag gcgatagaaa aagccttttt cctgcgctac
    28081 tttctcggca taacaaacat cagcggcata ataaacgtcc agccccggca ttttgttgaa
    28141 tccaacgact ttatcgacgg gatgttcgcg taaatgtttt tgcacccagg caaaatactc
    28201 cgcattgcgc ccgtgattgg tatgcgattt aaccggcact ttgatcagtt caaatacatc
    28261 agggcattcg ccttcccacg actgggtata aacccgaaca tgatgacctc gggcggcgac
    28321 tgtctgagca atacgcataa aatcgcgctg caaaccgcca aagggaaaat atttatataa
    28381 acaaaaagca acgatcataa cgacgtacct gtcgtggagg agggcagtaa tttatcgaca
    28441 gcagcaatga catctgccgc cggaataacc gaaagataca tctcatttcg gtcacgctga
    28501 tcgcgcgttg gcatttcccg gtaatctccc gcccagaatt gaatcatgtt atttgaccag
    28561 ggacgccaga aaatatgatc tgttgcgcca aacagcgata tcagcggcgt attaactgca
    28621 gcggcaatat gcgccggtgc ggaatcaacg ccaataaaca gctgcgcatg atcgattaac
    28681 gcaccaagtt ccgggaaggt cacctttcca gccagcgccg ttactggtgg cgtctggcat
    28741 ccctgcgcaa tttcattgac gcaggccaga tcgtctttat ctgggccgga cgtcagaacg
    28801 acttcataac cacgagcatg taaggcatca atcacagcgg aaaacttggc gttgtcccag
    28861 catttgaaga tttgccgcgc cgtaggttgg ataaccacat aattttgtcc aacaccagcg
    28921 tgatcaagtt cgcgacgcat acgtttccag cttgcaggcg ggtaactcat ggttgtctgc
    28981 ttcaccaacg aatcaagtcc caatggtgtc agcacggata agttactttc caccacattt
    29041 ccaccctgca acggcaccaa atgggtgaaa cttttacgcc aaaaagcaga ctgccgatga
    29101 tgataatcct gggaaatttt cacacgagca tttaataagc gaaccagtat agcaaccatc
    29161 cattgatcgg taagattgac gataaggtca tacttattgg cacgtaatac cttgatgaga
    29221 tgaaaaaagt tggcaatttt ttctgaggct tttgcttttt tattttttat gccgtagagc
    29281 gcgttaatct ctggattttc agacaggatc gggatggtgt cctgataaag cagcacatcg
    29341 atttttgcgt caaggtaatt ttttttcagc gaactaatga cgggagtagt taataacata
    29401 tccccatgaa aacgcatctt aatgagcaaa attcttcgaa atggcttatc cacaagtaac
    29461 tctttttgtt gagattatcc gattaaggtg aagcagaaaa agcacgctac cgccccaggc
    29521 tcaacagcta cctgaatact gataccgcac tgatattgac gcgctagtgt atcacttctt
    29581 tcgcggcaat ccgacccgaa tgatttttaa gcacattgtt gattttcaat aaagaaatca
    29641 atcaaataaa atcattagca accatattct ttcactgttg ctctgcgatt ccttcctgca
    29701 cgtacagcag aaatcaatga atcgcataaa aatacaaaaa aagtatagat gttcagcaag
    29761 aattggcggg taaattgcta tttaagtcaa aaacgggaaa agtaatggta aagccacagc
    29821 taaatacata gaatccccag cacatccata agtcagctat ttactatgct cgaattgctt
    29881 tacaccgccc ttctctacct tattcagccg ctgatctgga tacggctctg ggtgcgcgga
    29941 cgtaaggctc cggcctatcg aaaacgctgg ggtgaacgtt acggttttta ccgccatccg
    30001 ctaaaaccag gcggcattat gctgcactcc gtctccgtcg gtgaaactct ggcggcgatc
    30061 ccgttggtgc gcgcgctgcg tcatcgttat cctgatttac cgattaccgt tacaaccatg
    30121 acgccaaccg gttcggagcg cgtacaatcg gctttcggga aggatgttca gcacgtttat
    30181 ctgccgtatg acctgcccga tgcgctcaac cgtttcctga ataaagtcga ccccaaactg
    30241 gtgttgatta tggaaaccga actatggcct aacctgattg cggcgctaca taaacgtaaa
    30301 attccgctgg tgatcgctaa cgcgcgactc tctgcccgct cggccgcagg ttatgccaaa
    30361 ctgggtaaat tcgtccgccg cttgctgcgt cgtattacgc tgattgctgc gcaaaatgaa
    30421 gaagatggtg cacgttttgt ggcgctgggc gcaaaaaata accaggtaac cgttaccggt
    30481 agcctgaaat tcgatatttc tgtaactccg cagttggctg ctaaagccgt gacgctacgc
    30541 cgccaatggg caccacaccg cccggtatgg attgccacca gcactcacga aggtgaagag
    30601 agcgtggtca ttgccgcaca tcaggcattg ttacagcaat tcccgaattt attgctcatc
    30661 ctggtacccc gtcatccaga acgcttcccg gatgcgatta accttgtccg ccaggctgga
    30721 ctaagctata tcacacgctc ttcaggggaa gtcccctcca ccagcacgca ggttgtggtt
    30781 ggcgatacga tgggcgagtt gatgttactg tacggcattg ccgatctcgc ctttgttggc
    30841 ggttcactgg ttgaacgtgg tgggcataat ccgctggaag ctgccgcaca cgctattccg
    30901 gtattgatgg ggccgcatac ttttaacttt aaagacattt gcgcgcggct ggagcaggca
    30961 agcgggctta ttaccgttac cgatgccact acgcttgcaa aagaggtttc ctctttactc
    31021 accgacgccg tttaccgtag tttctatggc cgtcatgccg ttgaagtact gtatcaaaac
    31081 cagggcgcgc tacagcgtct gcttcaactg ctggaacctt acctgccacc gaaaacgcat
    31141 tgaggttgtt atgcaaaaac gggcgattta tccgggtact ttcgatccca ttaccaatgg
    31201 tcatatcgat atcgtgacgc gcgccacgca gatgttcgat cacgttattc tggcgattgc
    31261 cgccagcccc agtaaaaaac cgatgtttac cctggaagag cgtgtggcac tggcacagca
    31321 ggcaaccgcg catctgggga acgtggaagt ggtcgggttt agtgatttga tggcgaactt
    31381 cgcccgtaat caacacgcta cggtgctgat tcgtggcctg cgtgcggtgg cggattttga
    31441 atatgaaatg cagctggcgc atatgaatcg ccacttaatg ccggaactgg aaagtgtgtt
    31501 tctgatgccg tcgaaagagt ggtcgtttat ctcttcatcg ttggtgaaag aggtggcgcg
    31561 ccatcagggc gatgtcaccc atttcctgcc ggagaatgtc catcaggcgc tgatggcaaa
    31621 attagcgtag cgtttatgcc ggatggtatg ccatccggct cgcatgaatt acttctggca
    31681 ctgccgacaa taaaacgttg cccgctgcgc atgtttagtc gccacaatcg gcgtaccgca
    31741 cacccgacac ggctcacctt ttcgcccgta aacctgcaat tcctgcgcga aatagcccgg
    31801 tttaccatca ctttgcagaa aatctttcag cgttgtacca ccctgctcaa tcgaacgcag
    31861 caacaccgct ttaatcaccc gagctaacaa ttcacactct gccagcgaca gtgatgacgc
    31921 cagccgatcc ggatggatcc ccgccgcaaa cagtgattcg ctggcataga tattccctac
    31981 ccctaccacc agcttgttat ccatcagcca cggtttaatc gccgtttttt tcttcgcgca
    32041 cttctgatgc agatactcac cattgaaatc gtcgctaagc ggctccggtc caagatgggc
    32101 cagcacatta tgcccttcca gctctttggt ccacagccag gcaccaaagc ggcgcggatc
    32161 ggtgtagcgc agcactttac cgttgctcat caccaaatcc acatggtcat gcttttcagg
    32221 gggaagttct tctggaagga tgcgcaggct gccagacatc cccaaatgaa tgataatcca
    32281 gccctcaggc agctccagca gcagatattt agcccgccgc tgcacgctaa gcactggttg
    32341 atcgcttaat cggtagatct cttctgaaac cggccagcgc aagcgtccgt tgcgcaccac
    32401 cgcatgaaga atggttgcac caacgagatg cggttctatg ccgcgacggc tggtttcaac
    32461 ttcgggtaat tcaggcatag catctccagg aatgaacaga tgcaatcaat atgggggcaa
    32521 gcagataaca aaaaaccccg ccggagcgag gtttttcgtt acatcaaagc gagaattatt
    32581 tgattttcgc ttctttgtag atcacgtgct gacgaacaac tggatcgaat tttttcagtt
    32641 ccagtttttc cggcttagta cgtttgttct tcgtagtggt atagaagtga ccagtaccag
    32701 cagaagaaac cagcttgatt ttctcacgaa tacctttagc catgatttat ttcctctaag
    32761 tacttagtac ttttcgccac gggcacgcag ttcagccaga actgtatcga tgcctttttt
    32821 atcgattaca cgcatacctt tagcagatac gcgcagggtg acaaaacgct tctcgctctc
    32881 aacccagaaa cggtgagagt gcaggttcgg caggaaacgg cgtttagtcg cgttcagtgc
    32941 gtgggaacgg ttgttaccgg tcaccggacg cttgccagta acttggcaga ctcgggacat
    33001 gtctattctc caaaaatcaa attagctcga gcttcgtatg gggtattggc gcctcgtcag
    33061 gctttacagc ccggtcatcg cagttctatg tgaactctcg attgccaggc ccaaatgcca
    33121 aacccgagat tctcaaaggt ggcgtagtat acgctgactc agcgatgtgc tcaagtcccg
    33181 aacagacaaa gatcccgaag gatcgcgcat agcgggttaa atccagccgc gttcggcaaa
    33241 agaaacatac tctccacgcc caatcacgat atggtcgagc acgcgtaaat ccatgaactg
    33301 acaactcttt attatccgtt cagtaatgag tttatccgct ttactgggtt cagcacaacc
    33361 cgaagggtga ttatgtgcaa ggatcagcgc cgaggcgttt atttttatcg cttcgcggat
    33421 aatttcccga ggatggactt caacatggtt tagcgtgccg gaaaaaagac ggctatgcgt
    33481 tataacccgg tgttgtgagt cgagaaagat caccataaag atctcccgct cctcacccgt
    33541 gagctggctt tgtaaaaatt cccgcgtcat ctccgggctg agtaaagggc tttcttcacg
    33601 catccgcacg ttgtagtaac gtcgcgccag ttcagcaatc ccctttaact gggcaaattt
    33661 cgctacgcca attccatgaa caccactaaa ttgttcatat tcagaggtta acaagccata
    33721 aagagagccg aaattctcca gcatctcttt tgccagggtt aatacatctt taccgcgcgt
    33781 tccggtacgc agaaatagcg ccagcagctc gacatccgtt aaggcgctaa taccaaactt
    33841 cagcattttt tcgcgcggca tcaacagctg tgcattgttt ttcaccttca cctcctttgt
    33901 ggtgctcgca tcctgtcata gccgttccgt taaatcgacg gccagttttc aatcctggaa
    33961 agagcctcgc aaagtgaatc tcaggtgaag gcacgacaac aaaagtattg tgataaaatc
    34021 gccaacttct ggtgtcaccc aacaggaaaa atcatcatga gcctggccgg taaaaaaatc
    34081 gttctcggcg ttagcggcgg tattgctgcc tataaaaccc ctgaactggt gcgtcgtttg
    34141 cgcgatcgcg gggccgacgt ccgcgtagcc atgaccgaag cggcaaaagc ctttatcacc
    34201 ccacttagct tgcaggcggt ttctggttat cccgtttccg acagtctgct tgacccggca
    34261 gccgaagccg ctatgggcca tattgagctg ggtaaatggg ctgatttagt gattctcgcc
    34321 cctgccacgg cagatttgat tgcccgtgtt gctgccggaa tggcgaatga cttggtatcg
    34381 acgatttgtc tggctacacc tgcgcctgta gccgtgctcc ccgccatgaa ccagcagatg
    34441 taccgtgccg ctgccacgca gcataattta gaggtgcttg cttcccgtgg tttgctcatc
    34501 tgggggccag acagtggcag tcaggcttgt ggtgatatcg gtcctgggcg aatgctcgat
    34561 ccgttaacca ttgtggatat ggcggtggcg catttttcgc ccgtcaacga cctgaaacat
    34621 ctgaacatta tgattaccgc cggtccgacg cgtgaaccgc tcgatccggt gcgttatatc
    34681 tctaatcaca gctccggcaa gatgggtttt gctatcgccg ccgctgctgc ccgtcgtggc
    34741 gcgaacgtca cgctggtatc aggtccagtt tcactaccga cgccaccgtt tgttaaacgt
    34801 gttgatgtga tgaccgcgct ggaaatggaa gccgccgtga atgcttctgt acagcagcaa
    34861 aatattttta tcggttgcgc cgccgtggcg gattatcgcg cagctaccgt ggccccagaa
    34921 aaaatcaaaa agcaggccac gcagggtgat gaattaacaa taaaaatggt taaaaacccc
    34981 gatatcgtcg caggcgttgc cgcactaaaa gaccatcgac cctacgtcgt tggatttgcc
    35041 gccgaaacaa ataatgtgga agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat
    35101 ctgatctgcg cgaacgatgt ttcccagcca actcaaggat ttaacagcga caacaacgca
    35161 ttacaccttt tctggcagga cggagataaa gtcttaccgc ttgagcgcaa agagctcctt
    35221 ggccaattat tactcgacga gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat
    35281 tctggacccg cgcgttggga aggaatttcc gctcccgact tatgccacct ctggctctgc
    35341 cggacttgac ctgcgtgcct gtctcgacga cgccgtagaa ctggctccgg gtgacactac
    35401 gctggttccg accgggctgg cgattcatat tgccgatcct tcactggcgg caatgatgct
    35461 gccgcgctcc ggattgggac ataagcacgg tatcgtgctt ggtaacctgg taggattgat
    35521 cgattccgac tatcagggcc agttgatgat ttccgtgtgg aaccgtggtc aggacagctt
    35581 caccattcaa cctggcgaac gcatcgccca gatgattttt gttccggtag tacaggctga
    35641 atttaatctg gtggaagatt tcgacgccac cgaccgcggt gaaggcggct ttggtcactc
    35701 tggtcgtcag taacacatac gcatccgaat aacgtcataa catagccgca aacatttcgt
    35761 ttgcggtcat agcgtgggtg ccgcctggca agtgcttatt ttcaggggta ttttgtaaca
    35821 tggcagaaaa acaaactgcg aaaaggaacc gtcgcgagga aatacttcag tctctggcgc
    35881 tgatgctgga atccagcgat ggaagccaac gtatcacgac ggcaaaactg gccgcctctg
    35941 tcggcgtttc cgaagcggca ctgtatcgcc acttccccag taagacccgc atgttcgata
    36001 gcctgattga gtttatcgaa gatagcctga ttactcgcat caacctgatt ctgaaagatg
    36061 agaaagacac cacagcgcgc ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc
    36121 gtaatcctgg cctgacccgc atcctcactg gtcatgcgct aatgtttgaa caggatcgcc
    36181 tgcaagggcg catcaaccag ctgttcgagc gtattgaagc gcagctgcgc caggtattgc
    36241 gtgaaaagag aatgcgtgag ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc
    36301 agatcctggc cttctgtgaa ggtatgctgt cacgttttgt ccgcagcgaa tttaaatacc
    36361 gcccgacgga tgattttgac gcccgctggc cgctaattgc cgcgcagttg cagtaatatg
    36421 acgccggatg acttttcatc cggcgagttt ctttaaacgc caaactcttc gcgataggcc
    36481 ttaaccgccg ccagatgttc cgccatttcc ggcttctctt ccagataggc aatcagatct
    36541 ttcagggtga tgatagagat cactttgcag ttgtaatcac gctcaacttc ctgaatcgcc
    36601 gaaatctcgc cgcgcccgcg ttcctgacga tcgagcgaaa tcaacacgcc agcaagcgtc
    36661 gcgccattgg cctgaataat ctccatcgac tcgcgaatcg ccgttccggc ggtgatcaca
    36721 tcatctacca gcattacgcg tccttgtaac gcgctaccaa ccagattgcc gccttcaccg
    36781 tggtcttttg cttctttgcg gctaaagcag tacggcaggt ccaggtcgtg atgctccgcc
    36841 agtgccacag cggttgtggt ggcaatcggg atccctttgt aagcagggcc aaacagcaga
    36901 tcgaactcaa tgccggaatc caccaacgct tcagcgtaaa aacggcctaa cagtgccaga
    36961 tcgcgcccgg tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc
    37021 agcgtaaact cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc
    37081 tgatatggtt tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac
    37141 tcatcagtcg ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt
    37201 cgattccccc tcgggccagc gccaacaagg tgagtagctc ttcatgggtg aacggctcgc
    37261 cttctgccgt cccctgcact tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt
    37321 cggtctctgc ggctgagtct tcaacgtatt ccagatcgca aattgcttca ccgtttacaa
    37381 taccgacaga aactgcggct accatccctt tcatcgggtt ggttttcagc ttgccgtttt
    37441 ccaccagctt ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gacgcggtac
    37501 gagtgccacc atcagcctga agcacgtcgc agtccagagt aatggtgaac tcacccagcg
    37561 ctttcaaatc tactgccgcg cgaagagcac gggcgatcag acgctggatt tccattgtgc
    37621 gtccaccctg cttacctttc gccgcttcac gagcgttacg ggtgtgggta gaacgtggca
    37681 gcatgccgta ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc
    37741 cttcttcaat agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc
    37801 cttctgcatg ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac
    37861 ggcctgctgg acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg
    37921 gacttccggc ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct
    37981 ccttttataa acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat
    38041 caagggtgaa tgggggagcg caacctggga aatgcgctcg gtaaaccagc gttatctgga
    38101 aacttacttt cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gcgagcgtat
    38161 tcgttctcgc ctgacgcgcg gtaaagtgga atgtaccctg cgctatgagc cagatgttag
    38221 cgcacaaggt gagctgatcc tcaatgaaaa actggctaaa cagctggtaa ctgccgcgaa
    38281 ctgggtaaaa atgcagagtg acgaagggga aatcaacccg gttgatattc tacgctggcc
    38341 gggcgtgatg gcagcccagg agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc
    38401 gttggatggt acgctggacg actttatcgt cgcgcgcgaa accgaaggtc aggcactgaa
    38461 agcattgatc gagcagcgtc tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca
    38521 tatgccggaa attatgcaat ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca
    38581 ggttcagttg gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga
    38641 cgttgccgaa gagctggacc gcctcgaagc gcatgttaaa gagacgtaca acattctgaa
    38701 gaaaaaagaa gcggttggtc gtcgtctgga ctttatgatg caggagttca accgtgagtc
    38761 gaacactctt gcgtcgaaat ctatcaatgc cgaagtgaca aactccgcca tcgagctgaa
    38821 agtgttgatt gagcagatgc gcgagcagat tcagaatatt gagtaactcg taacaccaat
    38881 tctgactttt cagccttcac aaatgccgcc cactcaaaca gagcggcatt tttcttcccc
    38941 gcaacttcaa ttctgttttg cgtgcctgct ccagattttg cgatgttttt ttgcccagca
    39001 cactgagaac gtgagatact cacaactgta tataaataca gttacagatt tactttcttt
    39061 gcaatcgata tcacatggag tgggcaatga acgaacatca tcaacctttt gaagagataa
    39121 aactgattaa tgcaaacgga gcagaacaat ggtcagcaag acaacttggg aaactactgg
    39181 gttattcaga gtatcgtcac tttatacctg tattaacgcg cgccaaagag gcctgtgaga
    39241 acagtggtca cacaattgat gaccatttcg aggagatcct cgatatggtc aaaattggct
    39301 caaatgccaa aagagcatta aaagacatcg tactctcccg ctatgcctgt tacctggtag
    39361 tacaaaacgg cgaccccgcg aaaccggtca ttgcggcagg gcagacttat tttactatcc
    39421 agacccgacg gcaggagctt gctgacgatg aagcatttaa gcaacttcgt gaagacgaaa
    39481 agcgtctgtt tctgagaaat gagttgaaag aacataacaa acaattggtt gaggcagcac
    39541 agcaagctgg ggtagcgaca gctactgatt tcgccatatt tcagaatcat ggttaccagg
    39601 ggctgtatgg tggattagat cagaaagcca tccaccaact gaaggagctg aaaaagagtc
    39661 agaagatcct ggatcatatg ggttcaacag aactggcggc taatctcttt cgagctaccc
    39721 aaacagaaga aaaactcaag cgggatagcg tgaattcaaa acagcaagct aataccacac
    39781 actttgacgt gggtagcaag gtgaggcaaa ccattcagga acttggcgga accatgcctg
    39841 aggagttgcc gaccccgcag gtcagcatca agcagttgga aaacagtgta aaaattacag
    39901 agaagaaata g
//
LOCUS       DADKXM010000021        36324 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.021, whole genome shotgun sequence.
ACCESSION   DADKXM010000021 DADKXM010000000
VERSION     DADKXM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.021"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(26..205)
                     /locus_tag="JQR05_001120"
     CDS             complement(26..205)
                     /locus_tag="JQR05_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY5323333.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(289..1173)
                     /gene="yhdJ"
                     /locus_tag="JQR05_001121"
     CDS             complement(289..1173)
                     /gene="yhdJ"
                     /locus_tag="JQR05_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY5323334.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1259..1555)
                     /gene="fis"
                     /locus_tag="JQR05_001122"
     CDS             complement(1259..1555)
                     /gene="fis"
                     /locus_tag="JQR05_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY5323335.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1581..2546)
                     /gene="dusB"
                     /locus_tag="JQR05_001123"
     CDS             complement(1581..2546)
                     /gene="dusB"
                     /locus_tag="JQR05_001123"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY5323336.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2875..3756)
                     /gene="prmA"
                     /locus_tag="JQR05_001124"
     CDS             complement(2875..3756)
                     /gene="prmA"
                     /locus_tag="JQR05_001124"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY5323337.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLYDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRNNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3768..5219)
                     /gene="panF"
                     /locus_tag="JQR05_001125"
     CDS             complement(3768..5219)
                     /gene="panF"
                     /locus_tag="JQR05_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY5323338.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TVVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5209..5451)
                     /locus_tag="JQR05_001126"
     CDS             complement(5209..5451)
                     /locus_tag="JQR05_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY5323339.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5560..6909)
                     /gene="accC"
                     /locus_tag="JQR05_001127"
     CDS             complement(5560..6909)
                     /gene="accC"
                     /locus_tag="JQR05_001127"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY5323340.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6920..7390)
                     /gene="accB"
                     /locus_tag="JQR05_001128"
                     /gene_synonym="fabE"
     CDS             complement(6920..7390)
                     /gene="accB"
                     /locus_tag="JQR05_001128"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY5323341.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8368..9342)
                     /locus_tag="JQR05_001129"
     CDS             complement(8368..9342)
                     /locus_tag="JQR05_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5323342.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9494..11434
                     /gene="csrD"
                     /locus_tag="JQR05_001130"
     CDS             9494..11434
                     /gene="csrD"
                     /locus_tag="JQR05_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY5323343.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11739..12782
                     /locus_tag="JQR05_001131"
     CDS             11739..12782
                     /locus_tag="JQR05_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY5323344.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12848..13951
                     /gene="mreC"
                     /locus_tag="JQR05_001132"
     CDS             12848..13951
                     /gene="mreC"
                     /locus_tag="JQR05_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY5323345.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13951..14439
                     /gene="mreD"
                     /locus_tag="JQR05_001133"
     CDS             13951..14439
                     /gene="mreD"
                     /locus_tag="JQR05_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY5323346.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14448..15041
                     /gene="yhdE"
                     /locus_tag="JQR05_001134"
     CDS             14448..15041
                     /gene="yhdE"
                     /locus_tag="JQR05_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY5323347.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15031..16500
                     /gene="rng"
                     /locus_tag="JQR05_001135"
     CDS             15031..16500
                     /gene="rng"
                     /locus_tag="JQR05_001135"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY5323348.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16568..20368
                     /locus_tag="JQR05_001136"
     CDS             16568..20368
                     /locus_tag="JQR05_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY5323349.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20524..21969
                     /gene="tldD"
                     /locus_tag="JQR05_001137"
     CDS             20524..21969
                     /gene="tldD"
                     /locus_tag="JQR05_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY5323350.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22097..23026)
                     /gene="aaeR"
                     /locus_tag="JQR05_001138"
     CDS             complement(22097..23026)
                     /gene="aaeR"
                     /locus_tag="JQR05_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY5323351.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23209..23412
                     /locus_tag="JQR05_001139"
     CDS             23209..23412
                     /locus_tag="JQR05_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY5323352.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23420..24352
                     /gene="aaeA"
                     /locus_tag="JQR05_001140"
     CDS             23420..24352
                     /gene="aaeA"
                     /locus_tag="JQR05_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY5323353.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24358..26325
                     /gene="aaeB"
                     /locus_tag="JQR05_001141"
     CDS             24358..26325
                     /gene="aaeB"
                     /locus_tag="JQR05_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY5323354.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26417..26689
                     /locus_tag="JQR05_001142"
     CDS             26417..26689
                     /locus_tag="JQR05_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323355.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26745..27008)
                     /gene="yhcN"
                     /locus_tag="JQR05_001143"
     CDS             complement(26745..27008)
                     /gene="yhcN"
                     /locus_tag="JQR05_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY5323356.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27374..27844)
                     /gene="argR"
                     /locus_tag="JQR05_001144"
     CDS             complement(27374..27844)
                     /gene="argR"
                     /locus_tag="JQR05_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY5323357.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28279..29217
                     /gene="mdh"
                     /locus_tag="JQR05_001145"
     CDS             28279..29217
                     /gene="mdh"
                     /locus_tag="JQR05_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY5323358.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVILISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29279..30346)
                     /gene="degS"
                     /locus_tag="JQR05_001146"
     CDS             complement(29279..30346)
                     /gene="degS"
                     /locus_tag="JQR05_001146"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY5323359.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30436..31803)
                     /gene="degQ"
                     /locus_tag="JQR05_001147"
     CDS             complement(30436..31803)
                     /gene="degQ"
                     /locus_tag="JQR05_001147"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY5323360.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASILGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31957..32355)
                     /locus_tag="JQR05_001148"
     CDS             complement(31957..32355)
                     /locus_tag="JQR05_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY5323361.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32549..33676
                     /locus_tag="JQR05_001149"
     CDS             32549..33676
                     /locus_tag="JQR05_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY5323362.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33895..34323
                     /gene="rplM"
                     /locus_tag="JQR05_001150"
     CDS             33895..34323
                     /gene="rplM"
                     /locus_tag="JQR05_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY5323363.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34339..34731
                     /gene="rpsI"
                     /locus_tag="JQR05_001151"
     CDS             34339..34731
                     /gene="rpsI"
                     /locus_tag="JQR05_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY5323364.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35134..35772
                     /gene="sspA"
                     /locus_tag="JQR05_001152"
     CDS             35134..35772
                     /gene="sspA"
                     /locus_tag="JQR05_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY5323365.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35778..36275
                     /gene="sspB"
                     /locus_tag="JQR05_001153"
     CDS             35778..36275
                     /gene="sspB"
                     /locus_tag="JQR05_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY5323366.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
BASE COUNT         8743 a         9053 c         9590 g         8938 t
ORIGIN      
        1 cgaatgaatt aagtgtcatg taaattcaca ttctgtcctg aaaattcagt tcgtcacagt
       61 taaccagttt tagtggatta actgagttca tattacggca tttgtctgtt tctgttgtca
      121 gcagttcaat ccactgcatc agtaaagtat caaacagaaa aacgaagaca gcgaaaacga
      181 cgagccacca atacttgcga atcattacct gagcctgtgc atgtttaacc aagtctggca
      241 ggaaatatac acgaaatgaa taatctgaaa accaggctta cacttccttt actttgcaat
      301 gagatcgggg tcaacttcgc ttaaccggct tcgttttgac aggttgccgg tctttctttt
      361 tttcactttc gccagttctt ccgcggagta atgcgacgcg acatccagcc gtcgaagccc
      421 cattttgatg tactcgctgt tgatctcaat accaatgaat tttcgtccgc tggcgatggc
      481 tacggcaccg gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc ctgggttgga
      541 agaggcgaga ataatgcgtt tcagtaaggc tttcggtttt tgcgtcgggt ggttctcata
      601 ttcatccatc aaataacgca cgcgcggaaa atcccagacg ttacccggta ctttttgatg
      661 attgtatggc tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg atccggtttt
      721 ggcttctacc agaatagcat caccgttgaa tgtgtagttc tttgcgtctt tcaccatcat
      781 caggatgggt tcgtacatgg agccgtagtg ttttttcgct tgtactccag aactgtcata
      841 tgaccagacg atgcgacttt tgatggtaaa aagcttgcgg cactggagat cgataaaggg
      901 catgttttcc gtactgttca taatgtacat gctgccctgc tttttcagaa cgcggtggca
      961 ctctgcaatc acttcaaaca gccagtcgat aaacagatct tctttccagg cttcgatcag
     1021 accatcaaaa tttttaccga tgttatacgg tgggtcggca aagagcagat cgacgctttc
     1081 agtaggtagc tttttaagtt cggcaagggc atcaccgtga ataatggtct tagcttcatt
     1141 accaaaccgg gtcggttcac atcctgttct catggtcact ccctttgtga cacctataaa
     1201 aaaggcgctt ccccatgccg agtagcgcct ttttaatcaa gcatttagct aacctgaatt
     1261 agttcatgcc gtattttttc aattttttac gcagcgtacc acggttgatg cccatcatca
     1321 gcgcagcacg ggtctggtta ccacgggtgt attgcatcac catgtccaac aggggctgtt
     1381 ctacttcagc cagtaccagc tcatagaggt cattcacatc ctgaccattc agttgagcaa
     1441 aatagttctt cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt acctgatcct
     1501 gagagttaac ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg aacatagttc
     1561 tgtcagctct ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa cgcctccagc
     1621 tgttcgctgg catcctcaat ggcgttgaat gtgcgccgaa actggtcatt tggagcgtgt
     1681 tcctggagat accaggaaac gtgtttacgt gcaattcggt acccttttgc cggaccataa
     1741 aagtcatgca gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc caaaggcagt
     1801 gggggcagca actccccagt gtccagataa tgctggattt cccgaaagat ccagggtctt
     1861 ccctgagctg cgcggcctat catcagggca tccgcccctg tatagtcgag cacagctctg
     1921 gctttaagcg ggtcagtaat gtcgccattc gcgataaccg gaatggaaac tttctgctta
     1981 actgcccgaa tactgtcgta ctcagcttct ccattgaaca aacaggcgcg tgtacggcca
     2041 tgaatggtca gagcctgaat gccacagtct tcagccagtt gggcaatctc ttcgcagtta
     2101 cggtgttccg gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc cactgcattg
     2161 acgacctcgg taaggatcga tttaacgaca tccgggtact gcaagagggc tgaacctgcg
     2221 agcttgcgat tcactttttt agccgggcaa cccatattga tatcaataat ctgggcaccg
     2281 ctttccacgt taatacgtgc tgcatctgcc atttctttcg gagcgctacc agcaatttgc
     2341 acggtgcgaa taccgggttc atcaatgtgc accatccgta aacgagattt gtcgctttcc
     2401 caaacctgtg ggttagaaga catcatctcg gatactgtca atccggctcc catctcgtag
     2461 cacaacgtcc gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat caggcgattt
     2521 ctgagctgat attgtccgat gcgcatgagt taagaaatga ccatactgtg actgcaaggc
     2581 ggcgtatatt acgcattttt tgcacgagat gaaaggccaa actttgacca atcctctgag
     2641 atggatcaaa gaattgcatt taaaatgagc gtggtgcgat aattactcat aaaaatcatc
     2701 atattagaaa atagtgacta aaatttacac tcaaagaaat ttgagtaagt tctcaatttt
     2761 tctttatgaa tgaaaatttt ggcacgcaaa tttgcgtaaa taatcggcaa atttacgtgc
     2821 cttttgtgag cttgctcgca cttcgccccg cgtcacccta cggcgatgcg aaggttaatt
     2881 cttacgaccg gtaatacggc accactcttc tttttccacg accgggtcca gtgcgaagct
     2941 atcggcataa gcttcacaaa cgctctctgc ctggcttgcc agaataccgg aaaggcccag
     3001 caaaccgcct gaaaccggca ggacgctgat taacggtgcc agttcacgta atgggcctgc
     3061 aaggatgtta gcgaccacca cgtcggcttt catttcttct ggctgatctt tcggtaagta
     3121 gagttccaga cggtcagaaa cgccattacg ttcggcgtta ttgcggctgg cctgaatcgc
     3181 ctgcggatcg atatcaatac caatggcttt tgctgcaccc agtttcagcg ccgcgatcgc
     3241 cagaatgccg gaaccacagc caaagtcgat gactgtttta ccggttaaat cgaggctgtc
     3301 gagccattgc aggcacagag aggtggttgg atgggtaccc gtaccaaacg ccagccctgg
     3361 atctaacatc acgttgacgg cgttttcgtc cggcacatca cgccagctag ggcagatcca
     3421 cagtcgttca ccaaagcgca tcgggtggaa attatccatc cattcgcgct cccagtcttt
     3481 atcttctagt tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat ggttttccag
     3541 aatcgccacc acgtcgttca tatcggtttc agcgtcgtac agaccaatca catcggtgtc
     3601 gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt gggtatcctg
     3661 aaaagtgata gaaacggcac ccgcttccat cagcgcatcg ctaagatctt ccgcgttcgc
     3721 gccggtggtg ttcagtttca gttggatcca aggcatggca aaactcttta tttatcagta
     3781 gtcaaaacgg tagcttgtgg gacggatgta ccgaaacggt ttccgaccag gaaagccagc
     3841 aaacttagta gtaacgaggg cacgataggg tggaagccca ggtactgaat attcagcgtc
     3901 gcgagtacgg catacagcac gccgccaacg atcatcgcac ttagcgcgcc tttggcgttg
     3961 gcgcgttccc agtaaagacc cagcaccagc ggccacagga aaacggcttc cagcccaccg
     4021 aaggccagca aattcagcca gatgatcatt tctggcggct tccaggcggc aagcagcagc
     4081 aacgcgccga gaactaacgt aattaccgcc gacatccgct tcagacgcgt ctcgttttgc
     4141 atttgatccg gacggatatt cagatagaga tctttaatga tcgtagcgga actttgcagc
     4201 agttgggcgt taattgtcga catgatcgca gccataggtg cagccagaaa gatcccggca
     4261 gcaaaaggtg gcagcacttt taccattaac gtcgggatca ccagatccgg tacggtgaga
     4321 tcggggatca ctgcacgacc taacgctccg gccaggtgca taccgaacat caggattgcg
     4381 actacaaccg taccgatgat gatcccccgg tgtactgctt tgctgtcttt ataagagata
     4441 cagcgcaccg cagtatgcgg caggccaatc acgccaaagc acaccagtac ccagaacgac
     4501 gtcataaagg caggcgacag aatatcgtca gcgccttgtg gcgtaaccag ttgcggatcg
     4561 atggtttgca aggtctgtac tgcgttgctt aagccgccag cggcatgtac cacgccaata
     4621 agcagcacaa cggtgccaat cagcatcaca agcccttgca tggtgtcgtt cagcacgctg
     4681 gcgcgaaagc caccaaaggc ggtatataac gcaatgctga taccaaaaat cagcagcccg
     4741 gtttcataag gaatacccgc cgcggtttcc agcaggcgcg caccgccgat aaactgcacg
     4801 gtcatcgcac caacgaacgc aaccagcaaa ctcaaactcg ccagccacac cagaagacga
     4861 ctctggtagc gggcaaacag catatcgttc agcgtcactg cattgtagcg gcgcgcaaga
     4921 atcgcaaact tcttgccgag aataccgagt gaaagccaga cagcaggaag ctgaatcatc
     4981 gccagcaata cccagcccag cccgtattta taagcagctc ctggcccgcc gataaacgaa
     5041 ctggcactga tataggtcgc ggtgagcgtc atcgccagca caataccgcc catagagcgg
     5101 ctgccgagga aatactcatt aaggaaggtg ccggtgctcc gtttacgcat cgcataaacc
     5161 gagataccga acaccaccac cagataggcg accagcggta gaattacttc aagctgcatc
     5221 gtcatcctcc agtgggatat cgcgatagat aaatttcacc atcgcccagc acagtccaat
     5281 aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc cggtaaaacc
     5341 gggggcaacg ccaggtaagt aagcgtctac taaccaaact gccagataca aaagggtcag
     5401 ccccagcgcc cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca ttttttgtcc
     5461 ctgatgggtg aagaaagcga ggattgtacc ttatgggggt tgtcgatccc cagtaataaa
     5521 aaaggccgga aaatccggcc ttttgacgct tttgcagtct tatttttcct gaagaccgag
     5581 ttttttctcc agatagtgga tgttagtgcc accatgctgg aagttctcgt cattcatgat
     5641 gcggatctgc agatcaacgt tggttttgat accgtcgatg atcagctcct gcagcgcatt
     5701 cttcatgcgg gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct taccgatcat
     5761 tgagtcatag tacggcggta cggtgtagcc cgcgtagata tgagactccc aacgtacgcc
     5821 aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg cttggcagga aggtgttcgg
     5881 atcttcggcg ttgatacgac attccaccgc atggccgcga acgtgaactt cttcttgctt
     5941 gatcgacagc ggttgaccgg cagcgatacg cagctgttct ttgatcaggt caacgccggt
     6001 gatcatttct gtaaccgggt gttctacctg aatacgggtg ttcatttcga tgaaatagaa
     6061 ctcgccgttt tcgaacagga actcgaaagt acctgcaccg cgatagccga tatcaacaca
     6121 cgctttagcg caacgttcgc cgatgtagcg acgcagttcc ggggtaatgc ccggtgctgg
     6181 tgcttcttcg accactttct ggtggcggcg ctgcatggag cagtcacgtt ccgccagata
     6241 gatagcgttg ccctgaccgt cagccagtac ctgaatctcg acgtggcgag gattttccag
     6301 gtatttctcc atgtaaacca tatcgttgct gaaagcagct ttcgcttccg cacgggtcat
     6361 ggagatggat tgtgccagtt cagcgtcgcc gcgcactacg cgcataccgc gaccgccgcc
     6421 gccgccggag gctttgataa tcaccggata accaatgcgt ttagcaatgg cgcggttttt
     6481 atccatatcg tcgcccagcg ggccgtcaga accaggtacg caaggaacgc ccgctttttt
     6541 catcgccgcg attgcggata ctttgtcgcc catcaggcga atggtttctg ctttcgggcc
     6601 gatgaagata aagccggagc gttcaacctg ctcggcaaag ttggcgttct cggagaggaa
     6661 gccgtaaccc ggatggattg ctactgcgcc ggtgatttca gcggcgctga tgattgccgg
     6721 gatgttcaga taacttttta ctgacggagc agggccaata cagaccgttt catctgccag
     6781 taatacgtgt tttagatcgc gatccgcgct ggagtgcaca gcgacagtct tgatgcccag
     6841 ttctttacag gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa taacaatttt
     6901 atccagcatg ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat tctaccggtt
     6961 gtccactttc gaccagaatt gctttcacgg taccggattt gtccgcttcg atctggttca
     7021 tcattttcat ggcttcaacg atgcacaggg tatcgcccac gttgactttc tgacccactt
     7081 cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc atcggggaac
     7141 gtacgatgtg accactgatt tccgctgctg ctggcgcttc catggaagga acggtcgccg
     7201 gagcggctgc gttagattga gctggctgct gcatcattgg tgcagcgtaa gcttgttgca
     7261 tcacagggaa acttgcggca ggagctgcac ggctaatgcg tactgactct tcgccttcag
     7321 aaatttccag ttcggagatg cctgattctt caaccagctc gatcagtttt ttaatcttac
     7381 gaatatccat gagtgggttc cgtactcttt gtttagtgtg attgtgacag gcgtttcacc
     7441 gccgtctgta aagcgtatgc cccgacagct gtatgcatag cgataaattc cagcaggccg
     7501 ggacgccggt ctattttgcc ccagagtgtc aatgttagac ttgacggaca ttgtgcagcg
     7561 atctgctgct atcctccggc aaaaaacaaa atataccttc gttgcgccaa tgtcaccttt
     7621 tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca cattataacg atttcgtagc
     7681 aattggcagc taaatactgg tcttatcagg gaagataatc aacagctaac atgtaaataa
     7741 ccttcaacac cgtgtaattt gtaacaagcc gcacaattca cgaaattagc gccatcatcg
     7801 acggaacttc ttataacgta aggtgaaaag ctgaaaagcc agccctgcga agatgagggg
     7861 ctgcaatgag ataatcttca cagaccacaa ataatgtagg gatgccagga tcgcgacaag
     7921 atagacgaca ttacgcagcg gttgctgggg attacccaat tttcactgca tcgtctggat
     7981 agatgtgaat gctaaagcac gcaaaatcaa ccaactgaca attctcagca ttaaataggt
     8041 tcgagtggtt aatttgtagt caaataaaat cagattattc accccatggt accagaaatc
     8101 agtaacaggt taagcgtagt gtcgcccaga caaagcacca taaacctaac aggcgacgga
     8161 ggcgtatcaa taatggctgt ttagaggagt atcaatgcgc atcaatttgt ggtcagtcga
     8221 aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt gttcgacgaa
     8281 agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca aaatgagttc
     8341 cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc gaccctggat
     8401 ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata tctcttttgc
     8461 cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg cttgtgcgcg
     8521 gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac gcagaataaa
     8581 tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg ccacgcagcc
     8641 gccgtaattc atttgcgcta gcacttttgc cagcactttg tcgccaacag tgtcaattgc
     8701 cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac gagggagaat
     8761 acggctggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg aaacggcaac
     8821 gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc caccactggc
     8881 acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca gcgccatcac
     8941 acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg cgtccagccc
     9001 ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca gcccacccca
     9061 gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat gaaaacgcgg
     9121 atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg gaaaattacg
     9181 gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc tcgaccagtg
     9241 aacatcgacc gtgacatcgc cctccggcag gcgactttcg tccagagtct gtactgatgc
     9301 gagagttttg ccgtcctgct gttctaaaag taacgcctgc ataagtggtc ctcatgtgca
     9361 tgatggattg gaaaataatt tctgaagact atactcgcta atggaaaagc aaagccgatg
     9421 aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc tcactatcgg
     9481 agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc tgctcaccgg
     9541 gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaacg ccattcagta
     9601 taagtttagt catcgcgttc aggcggtggc gacggcgatt gatacccacc ttgtgtcgaa
     9661 tgacttcagc gcattaaggc cacaaattac cgaattaatg gtgtcagcag atatcgttcg
     9721 tgtagacctg ctgcatggtg ataaacaggt ttataccctg gccagaaatg gtagttatcg
     9781 tccggttggc accagcgatc tgtttcgtga actgagcgtt ccgttgataa agcatccggg
     9841 gatgtcgttg cgtctggttt atcaggatcc gatgggcaac tatttccatt cgttgatgac
     9901 caccgcgccg ctcacggggg cgattggctt tatcattctt atgctctttc tggcggtacg
     9961 ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta ctcgtatctt
    10021 aaacggtgag cgtggctcta atgtgttggg aaccatctat gaatggccgc ccagaaccag
    10081 cagtgcgctg gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac acagccgtct
    10141 tgatacgctg atccgctctt atgccgcaca ggacgtgaaa accggcctca ataaccgact
    10201 ctttttcgat aatcagttag caacgttact ggaagatcag gagaaagtag gtacccacgg
    10261 gatcgtgatg atgattcgtc tgccggattt caatatgttg agcgatacct gggggcacag
    10321 ccaagttgaa gaacagttct tcactctgac gaatctgctg tcgacattta tgatgcgcta
    10381 ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt taccgcaccg
    10441 gacgttaaaa gaggcagaga gcatagccag tcagttaatc aaagccgtcg ataccttgcc
    10501 gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgtg cctggcgtag
    10561 tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta atgcgggatt
    10621 gcagggtggc aatagctggg ctatttatga tgactcgttg cctgaaaaag gacgcggtaa
    10681 tgttcgctgg cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc gcctttatca
    10741 aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt gccgcatctt
    10801 cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc agtttggctt
    10861 atcggaagag tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc gttactggcc
    10921 agaggaaaat ctggcgattc aggttaccgt tgagtcgctg attcgtccgc gttttcagcg
    10981 ttggctgcgc gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca taattattga
    11041 acttgcagag gccgatgtag gtcaacatat cagtcgttta caacctgtta ttcgtttagt
    11101 gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg taagcaccag
    11161 ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg tcaggaacat
    11221 tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct gctccgggac
    11281 cagcacccac gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga ccctgattca
    11341 gcgcggtgtt acaggcgggc aaggggattt tttcgcgtcc tcacagccac ttgatactaa
    11401 cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatccg ttttcacgta
    11461 gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat tgttgcagca
    11521 catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg ctgatgcatt
    11581 aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca ctaattttca
    11641 ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa gtaagcggat
    11701 tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa tttcgtggca
    11761 tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt tatgtaaaag
    11821 gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat cgtgccggtt
    11881 caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc cgtacgccgg
    11941 gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc ttcgtgactg
    12001 aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg cgtccaagcc
    12061 cgcgcgttct ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc gcaattcgtg
    12121 aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg atggctgccg
    12181 caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt gatatcggtg
    12241 gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc tcttccgtgc
    12301 gcattggtgg tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt aattacggtt
    12361 ctctgatcgg tgaagccacc gcagaacgta tcaagcacga aatcggttcg gcttacccgg
    12421 gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaactt ggcagaaggt gttccacgcg
    12481 gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg accggtattg
    12541 tgagcgcggt aatggttgca ctggaacagt gcccgccgga actggcttcc gacatctccg
    12601 agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac cgtttgttaa
    12661 tggaagaaac cggcattcca gtcgttgttg ctgaagaccc gctgacctgt gtggcgcgcg
    12721 gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc agcgaagagt
    12781 aatcggatgc aggcagggga agcgtctgtt taccctgcct ggtctgatac gagaatacgc
    12841 ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc ttattctggc
    12901 ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt tcagtcaaat
    12961 ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg ctcctcgtga
    13021 attgctggat ggcatatcgc agacgctggc ctcgcgtgac caactagaac ttgaaaaccg
    13081 ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg gacaatacaa
    13141 acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg atgagcagaa
    13201 aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag ttgttatcga
    13261 taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca aaggtgttgt
    13321 tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt gtgatgcgac
    13381 ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag ccggtaacgg
    13441 ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc gtgttggtga
    13501 tgtgctggtg acctccggtc tgggcggtcg tttcccggaa ggctatccgg tcgcggttgt
    13561 ctcttccgta aaactcgata cccagcgcgc ttatactgtg atccaggcgc gtccgactgc
    13621 aggactgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta acggcgctaa
    13681 cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc agatgatgcc
    13741 gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg caacggggat
    13801 cgctcagccg actccgcagc aaccggcgac aggaaatgca gctactgcgc ctgttgcgcc
    13861 gacacagccc gctgctaatc gctctccaca aagggctacg ccgccgcaaa gtggtgctca
    13921 accgcctgcg cgtgcgccgg gagggcaata gtggcgagct atcgtagcca gggacgctgg
    13981 gtaatctggc tctctttcct cattgcgctg ttgctgcaaa tcatgccctg gccggataac
    14041 ctgattgttt tccggccaaa ctgggtgtta cttatcttgt tgtattggat cctggccttg
    14101 ccccatcgcg taaatgtggg cacaggtttt gtgatgggtg ccatactgga tctgatcagc
    14161 ggctcgatgc ttggcgtacg cgtattggcg atgagcatca ttgcttacct ggtggcgctg
    14221 aaataccagc ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt catgttgctt
    14281 tcgctggtgg tggatattat tgttttctgg gcagagtttt tagtgattaa cgtctctttc
    14341 agaccggaag tgttctggag tagtgtagtc aatggggtgc tctggccgtg gattttcttg
    14401 ctgatgcgta aagtccgtca gcagtttgca gtgcaataaa ggtttctatg acttctctgt
    14461 atttagcttc cggttctccg cgtcgtcagg agttacttgc gcaacttggc gtgacctttg
    14521 aacgtattgt tacgggcatt gaggagcagc gtcagccgca ggagagcgcg cagcagtatg
    14581 ttgtgcgtct ggcacgcgag aaagcacagg cgggtgtcgc gcaaacggcg caggatctcc
    14641 cggtgctggg tgcggatatt atcgttatcc tgaacggaga agtgctcgag aaaccgcgcg
    14701 acgcagagca tgcggcgcag atgttgcgca aattatcggg tcagacccat caggtgatga
    14761 cggcagtggc gttggccgac agccagcaca ttctcgattg cctggtggtc accgatgtga
    14821 ctttcagaac gttaacagac gaagacatcg cgggctatgt cgccagcggt gaaccgttag
    14881 ataaagcagg tgcatacggt attcaggggc tgggtggctg ttttgtcagg aagataaatg
    14941 gcagctatca cgccgtagtc ggcttaccgc tggttgaaac gtatgaatta ttaagtaatt
    15001 ttaacgcact gcgtgagaaa agggataaac atgacggctg aattgttagt aaacgtaacg
    15061 ccttcggaaa cgcgagtggc gtatattgat ggcggtattc tgcaggaaat tcatattgaa
    15121 cgtgaggcgc gacgcggaat agtaggcaat atctacaagg gtcgtgtaag tcgtgtactt
    15181 ccgggtatgc aggcggcttt tgtagatatt gggctggata aagccgcgtt tcttcatgca
    15241 tccgacatca tgccgcatac cgaatgtgtg gcgggtgaag aacaaaagca attcacggtg
    15301 cgcgacatct cggaactggt tcgtcagggg caagatctga tggtgcaggt ggtgaaagat
    15361 ccgcttggca ctaaaggtgc gcgcctgacc accgatatca cgctgccttc tcgctatctg
    15421 gtatttatgc caggggcttc tcacgttggg gtttcccaac gaattgaaag cgaatcagaa
    15481 cgtgaacgcc tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg gtttatcatc
    15541 cgcaccgcag cggaaggggt tggcgaggct gaactggcct ccgatgccgc ttatctgaaa
    15601 cgcgtctgga ccaaagttat ggagcgtaaa aagcgcccgc agacccgtta tcagctgtac
    15661 ggcgaactgg cgctggcgca gcgtgttctg cgtgattttg ccgacgccga actggaccgc
    15721 attcgcgttg actcacgcct gacttacgaa gcattgctgg agtttacctc ggagtacatt
    15781 cccgagatga ccagcaagct ggagcattac accggacgcc agccgatttt cgatctcttt
    15841 gatgtcgaaa acgaaatcca gcgagcgttg gaacgcaaag tagaactgaa atccggtggt
    15901 tatctgatta tcgaccagac cgaagcgatg accaccgtgg acatcaatac cggagcgttt
    15961 gtcggtcatc gcaatctgga cgacactatt ttcaatacca atattgaagc gacgcaggct
    16021 atcgctcgcc agttacggtt gcgtaatctg ggcgggatta tcattattga tttcatcgat
    16081 atgaataatg aagatcaccg ccgccgcgtg ctgcattcgc tggagcaggc gttgagcaaa
    16141 gaccgggtga aaaccagcgt taatggtttt tcggcgctgg ggctggtgga gatgacgcgt
    16201 aaacgcaccc gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac ctgccacggt
    16261 cgcggaacgg tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga gattgttcgt
    16321 gtccaccatg cttacgactc cgaccgtttc ctggtctatg cttctccggc agtagccgaa
    16381 gccttgaaag gcgaagagtc acactcgctg gcggaagtgg aaattttcgt tggcaaacag
    16441 gttaaagtac aaattgaacc gctctataac caggagcagt ttgacgtcgt aatgatgtaa
    16501 acagatgctg ggccgccatc cggcaaaggg tttttgagtc acatttttag cagacaagga
    16561 gtgacgggtg aggcgattgc cggggatttt actgcttact ggagccgcgc tcgttgtgat
    16621 cgctgccctg ctggttagcg gcctgcgtat tgctttaccg catcttgacg cctggcgtcc
    16681 ggaaatcctc aacaaaatag aatccgcgac tggcatgccg gtagaagcca gtcagctctc
    16741 agccagctgg cagaattttg gcccgacgct tgaagcacac gacatccgtg cagaactaaa
    16801 agatggcggc gaattttcgg ttaaacgcgt tactctggcg ctggatgtct ggcagagcct
    16861 gttacatatg cgctggcagt ttcgcgacct cactttctgg cagctgcgct ttcgcaccaa
    16921 cactcctatc accagcggtg gtagcgatga cagtctggaa gccagtcaca tcagcgatct
    16981 gtttcttcgt caatttgacc atttcgatct ccgcgacagt gaagtcagtt ttctgacgcc
    17041 atccggtcag cgcgccgagc tggcgatccc acaactcacc tggctgaacg atccacgtcg
    17101 acaccgtgcg gaaggcctgg taagcctctc cagccttacc ggacagcacg gcgtgatgca
    17161 ggtgcgcatg gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg tctggctaca
    17221 ggcggatgac atcgacctga agccgtggct cggtaaatgg atgcaggaca atattgctct
    17281 ggaaacggca cagttctccc ttgaaggctg gatgacgatc gacaaaggcg atgtaaccgg
    17341 cggtgacgtc tggctgaaac agggcggtgc cagctggttg ggcgagaagc aaacgcatac
    17401 gctgtcggtg gataatctga ccgcgcatat tacgcgtgaa aatccgggct ggcagttctc
    17461 tattcccgat acacggatca cgatggacgg caaaccctgg ccgagcggag cattgacgct
    17521 ggcctggata ccggaacagg acgttggcgg caaagacaat aaacgcagtg acgaactccg
    17581 gattcgcgct agtaatctgg agctggcagg cctggagggc atacgcccgc tggccgcgaa
    17641 actttcacct gcactgggtg atgtttggcg ctccacacaa ccgagcggca agattaacac
    17701 tctggcgctg gatatcccgc ttcaggcggc agacaagacc cgttttcagg catcgtggag
    17761 cgatctggcc tggaagcaat ggaaattatt accgggtgcg gaacacttct ccgggacgct
    17821 ttccggcagc gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa agatgcctta
    17881 cgaaacggta ttccgtgcgc cactggaaat cgccgacggc caggcaacta taagctggct
    17941 gaacaatgac aaaggtttcc agctggatgg gcgtaatatt gacgttaaag ccaaagccgt
    18001 ccatgcgcgc ggcggttttc gttacctgca acctgctaac gatgaaccct ggctgggtat
    18061 tctggctggc atcagtaccg atgatggttc acaagcctgg cgctatttcc cggaaaactt
    18121 gatgggtaaa gacctggttg attatttaag tggtgcgatt cagggcggtg aagcggataa
    18181 cgcgacgctg gtttatggtg gcaatccgca actcttcccc tataaacaca acgaaggtca
    18241 gtttgaagtg ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg actggcctgc
    18301 attaactaac cttgatattg aactggactt tattaacgac ggtttatgga tgaaaaccga
    18361 tggcgttaat ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc ctgactactc
    18421 aaaagaaaaa ctgctgattg acgctgacat taaaggtccg ggtaaagccg ttggccctta
    18481 ctttgatgag acaccgctga aagattctct gggtgcgacc ctgcaagaac tccagctcga
    18541 cggcgatgtg aatgctcgct tacatcttga tatcccgctg aacggcgaac tggtaaccgc
    18601 gaaaggtgaa gtgacgctgc gtaataacag tctgtttatc aaaccactcg acagcaccct
    18661 gaaaaatttg agcggtaaat tcagctttat caatggcgat ctgcaaagtg aaccactgac
    18721 agcaagctgg tttaatcagc cgttgaacgt ggatttttcc accaaagaag gggcaaaagc
    18781 ctaccaggtt gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaaccg gcgttctgcc
    18841 tgaagcggtg aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag tgggcattga
    18901 tctgccttat catgctggcg cgacctataa cgtagagctg aacggcgatc tgaataatgt
    18961 gagtagtcac ttaccttcac cgttagccaa acctgcgggt gaaccactag cggtaaacgt
    19021 taaggttgat ggcaatctca acagctttga attaaccgga caggctggtg cggataacca
    19081 tttcaatagc cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta tttgggcggc
    19141 agacagtaaa acgctcccgc cgttgccgga acaaagtggc gttgaactta atatgccgcc
    19201 gatgaatggt gccgagtggc tggccctgtt ccagaaaggc gctgcggaga gtgtcggtgg
    19261 tgcagcgagt ttcccacaac acataacgtt acgtacgcct atgttgtcac tgggaaatca
    19321 gcaatggaat aacctgagta ttgtttcgca accgacggca aatggcaccc tggttgaggc
    19381 gcaagggcgt gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt ggctggcgaa
    19441 tatcaaatat ctttattaca acccgagcgt ggcgaaaact cgtggtgatt caacgccgtc
    19501 atcacctttc ccgacaacgg agcgcattaa cttccgtggc tggccggacg cacaaatacg
    19561 atgcacagag tgctggttct gggggcaaaa attcggtcgc attgacagtg atatcaccat
    19621 ttctggcgat acgttaacgc tgaccaatgg actgattgat actggtttct cgcggcttac
    19681 tgccgatggt gaatgggtta ataatccggg gaatgaacgt acctcgctga aaggaaaact
    19741 gcgtgggcag aaaattgatg ccgccgcaga attttttggt gtcacgacgc ccatacgcca
    19801 gtcgtcattt aatgtggatt acgatttaca ctggcgcaaa gcaccctggc agccagatga
    19861 agcgacgttg aatggcatca ttcatactca actgggtaaa ggcgaaatta ccgaaatcaa
    19921 taccggacat gccgggcaat tgctgcgctt attgagcgta gatgccctga tgcgtaagct
    19981 gcgttttgat ttcagagaca cttttggcga agggttctat tttgactcca ttcgcagcac
    20041 cgcgtggatt aaagacggcg ttatgcacac cgacgacacg ctggtggatg gcctggaggc
    20101 ggatatcgcc atgaaagggt cggtaaatct ggtacgtcgc gacctgaata tggaagcggt
    20161 tgtcgcacca gagatttctg cgacggtggg cgtggctgcg gcttttgcgg ttaaccccat
    20221 tgttggcgcg gcagtgtttg ccgccagtaa agtgctgggg ccgctgtgga gcaaagtctc
    20281 cattttgcgc tatcacattt cgggtccgct ggacgatccg caaatcaacg aagtgttgcg
    20341 ccaaccgcgt aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc cccaatctca
    20401 tatgataatc gttgccaaag gccaacgagc cggaacataa ctgtaggccg gatagggcgt
    20461 tcacgccgca tccggcagcc gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa
    20521 acgatgagtc tgaacctggt aagtgaacaa ttgctagcgg cgaacggcct gaaacatcag
    20581 gacttgttcg cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg cgatctctat
    20641 tttcagtcga gctatcacga atcctgggtt ttagaagacc gcattattaa agatggttct
    20701 tacaacatcg atcagggcgt tggcgtgcgt gcaatcagcg gtgaaaaaac cggatttgct
    20761 tacgctgacc aaatcagcct gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc
    20821 gtccgtgata gtggtgatgg caaagtacag acgctgggcg cggtagagca tagcccgttg
    20881 tatacctcgg tagatccgct gcaaagcatg agccgtgaag agaagctgga tatcctgcgt
    20941 cgcgtcgata aggttgctcg cgaagcggac aagcgcgtac aggaagtgac tgccagcctc
    21001 agtggtgtct atgaattaat tctggttgcg gccaccgacg gcacgctagc ggcggatgtc
    21061 cgtccactgg tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa acgcgaacgc
    21121 ggtgccagtg gcggcggcgg tcgttttggt tatgagttct tccttgccga tcttgacggc
    21181 gaagtccgtg cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt caatctttct
    21241 gccgttgctg caccagcggg caccatgccg gtagtacttg gcgcaggttg gccgggcgtg
    21301 ctgttgcatg aagcggtagg tcacggtctg gaaggcgact tcaaccgccg tggcacttca
    21361 gtatttagtg gacaggtcgg ggagctggtg gcttcagaac tgtgtaccgt ggttgatgac
    21421 ggcacgatgg tcgatcgccg tggttcggtg gcgattgatg acgaaggtac gccaggccag
    21481 tacaacgtgc tgattgagaa cggcattctg aaaggctaca tgcaggataa actcaacgcg
    21541 cgtttgatgg gaatgacgcc gactggcaac ggtcgccgtg aatcctacgc ccatctgccc
    21601 atgccgcgta tgaccaacac ctatatgctg ccgggtaaat cgaccccgca agaaattatt
    21661 gaatccgttg agtacggtat ctatgcaccg aactttggtg gcggtcaggt ggatatcacc
    21721 tccggcaaat tcgttttctc cacttcagaa gcatatctga ttgaaaacgg taaagtaacg
    21781 aagccggtga aaggcgcaac gttgattggt tccggtatcg aaaccatgca gcagatttcg
    21841 atggttggca acgacctgaa actggataac ggcgtgggtg tctgcggtaa agaagggcaa
    21901 agtttgccgg ttggcgtggg ccagccaacg ttgaaagtcg ataacctgac tgttggcggt
    21961 actgcgtaat aatttcatta ttttcagaag gataattaat ctttctacgt gcacgaacgg
    22021 tcccctcgct cctctggggt tagggtgagg ggaacccgtt ggcacaggtt tgtagaacgt
    22081 aacagtacaa tatgaattac ttctctttcc cgcgcccgtg catctcctga aacaatttac
    22141 cgacctcaac aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc agcggcagct
    22201 tatctttttc ggtatataac gcataaaccg ggcgtggatc tgactgataa cgcggcagca
    22261 ggatctccag ctccccacga ttgatctcgt tgatcaccca catcagcggc acgtaggcga
    22321 tcccggcacc cgccgttagc cagcgcacca gcgtcatcgg atcattagtc acaaatctcc
    22381 cttgcgggat caggcgagtc gagatccctt ccggtgcaat cagttcaaat tcattgtcgg
    22441 gccgcacgct gtattcaagc catgaatggc tacttaaatc ggcgggtttt tccggtatgc
    22501 cgtattgtgt gagatagctt ttcgcggcgc acaccaccat tggcatcgcg ccaagacggc
    22561 gggaaaacag gctggaatcc tgcaacgcgc cgacgcggat caccacatcc agaccgtcgg
    22621 caatcaggtc gggggctgga attccggtaa ccagattggc gctcaaacct gggtattctt
    22681 tcagcatttt ggctgtcagc ccggcgagaa cattttgtgc catagttgaa gaacagccaa
    22741 tgcgtagcgt cccgatgggg gtgttattga aggcatacag ttgctcatga acatcctgca
    22801 cttcatgaag catacgacgg cagccctggt agtaaattct accggcttcg gtcaggccaa
    22861 tgctgcgtgt gctacggttt aacagcttta cctgcaactc atcttccagt tttgacaccg
    22921 tctgactgat ggacgaaacg ctcatctgta gctgtctggc ggcggcggta aaagagccaa
    22981 attcaactac tttggcaaac accgacatgc gttttagtcg ttccattatt cactctgact
    23041 taaaagtgat ttagatcaca taatatagat aacagcataa cagttacgct aatatattaa
    23101 atatcaatct acagcaatgt tgctctcgcc cggctttcca tgcccttctc tgcggcgaca
    23161 gatgctgaaa ataataacgc ctgctctctc tatacaacca aggtcaacat gagtctgttt
    23221 cccgttatcg tggtgtttgg gctgtccttc ccaccgatat tttttgaatt gcttttatca
    23281 ctggcgattt tctggctggt gcgccgggta cttgtgccaa caggtatcta cgactttgtc
    23341 tggcatccgg cgttgttcaa caccgcgctc tattgctgct tgttttattt gatatcgcga
    23401 ctgttcgttt gaggttgaag tgaaaacact aataagaaaa ttctcccgta cggccatcac
    23461 ggtcgtatta gtcattctgg ccttcatcgc aatttttaat gcctgggtct attacaccga
    23521 atccccctgg acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg cgccggacgt
    23581 ttctggactc attacccagg tgaatgttca tgataaccag ctggtgaaaa aaggacagat
    23641 actgttcacc atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc aagccgatgt
    23701 tgcttattat caggtactgg cacaggagaa acgccaggag gccggacgtc gtaaccgtct
    23761 cggtgtgcag gcgatgtctc gcgaagagat cgaccaggct aacaacgtac tacaaacggt
    23821 tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg atcttgaacg
    23881 cacggtgatc cgagcgccag cagatggctg ggtgaccaac ctcaacgtct ataccggtga
    23941 gtttattact cgaggatcaa cggcggttgc gctggtgaaa cagaactcct tctatgtact
    24001 ggcctatatg gaagaaacta agctggaagg ggtgcgtccg gggtatcgtg cagagatcac
    24061 gccgcttggc agtaacaaag tgctgaaagg gactgttgat agtgttgccg ctggggtcac
    24121 caacgccagc agcacgcgtg acgacaaagg gatggcgacc atcgactcta accttgaatg
    24181 ggtgcgtttg gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc aagagaacat
    24241 ctggcctgcg ggcaccactg ctacagtggt ggtcactggc aaacaagatc gcgacgaaag
    24301 ccaggattcg ttcttccgta aaatggccca tcgcctgcgt gagtttggtt aatcacgatg
    24361 ggtattttct ccattgctaa ccaacatatt cgctttgcgg taaaactggc gaccgccatt
    24421 gtactggcgc tgtttgttgg ctttcacttc cagctggaaa cgccacgctg ggcggtactg
    24481 acagcggcga ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc gtattctggc
    24541 gctattcgct atcgtggctt tttgcgcatc atcggcacat ttattggctg tattgccgga
    24601 ctggtgatca tcattgcgat gatccgcgca ccattattga tgattctggt gtgctgtatc
    24661 tgggccggtt tttgtacctg gatatcctcg ctggtacgaa tagaaaactc gtacgcgtgg
    24721 gggctggccg gttataccgc gctgatcatt gtgatcacca ttcagccgga accattgctt
    24781 acgccgcagt ttgccgtcga acgttgtagc gagatcgtta tcggtattgt gtgtgcgatt
    24841 atggcggatt tgctcttttc tccgcgatcg atcaaacaag aagtagatcg agagctggaa
    24901 agtttgctgg tcgcgcaata tcaattaatg caactctgta tcaagcatgg cgatggtgaa
    24961 gttgtcgata aagcctgggg cgacctggtg cgacgcacca cggcactaca aggcatgcgc
    25021 agcaacctga atatggaatc ttcccgctgg gcgcgggcca atcgacgttt aaaagcgatc
    25081 aatacgctat cgctgacgct gattacccaa tcctgcgaaa cttatcttat tcagaatacg
    25141 cgcccggaat tgatcactga tactttccgc gaattttttg acacgccggt agaaaccgcg
    25201 caggacgtcc acaagcagct caaacgcctg cggagagtta tcgcctggac cggggaacgg
    25261 gaaacgcctg tcaccattta tagctgggtc gcggcggcaa cgcgttatca gcttctcaag
    25321 cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag aagagatcct gcaaggcgaa
    25381 ccggaagtaa aagtagagtc agccgaacgt catcatgcaa tggttaactt ctggcgaacc
    25441 acactttcct gcattctggg cacgcttttc tggctgtgga cgggctggac ttccggcagt
    25501 ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc gaatccacgc
    25561 atggtggcga tcgactttat ctatgggacg ctggccgcgc tgccgttagg gctgctctac
    25621 tttttggtga ttatccctaa tacccaacag agcatgttgc tgctgtgtat tagcctggca
    25681 gtgctgggat tcttcctcgg tatagaagta cagaaacggc gactgggctc gatgggggca
    25741 ctggccagca ccataaatat tatcgtgctg gataacccga tgactttcca tttcagtcag
    25801 tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac cgttattttg
    25861 ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac tgcttaatca gtttgtttct
    25921 gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta aagagaacca cctcccggca
    25981 ctttatcagc agctgttttt gctgatgaat aagttcccag gggatttgcc gaaatttcgc
    26041 ctggcgctga cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat cccggttaac
    26101 gaggatttat cggcgtttca ccgacaaatg cgccgcacag cagaccatgt gatatctgcc
    26161 cgtagcgatg ataaacgtcg tcggtacttt ggccagttgc tggaagaact ggaaatctac
    26221 caggaaaagc tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt tcatcggctg
    26281 gcggggatgc tccataagta tcaacatgcg ttgaccgata gttaagtcaa aaccgacgcc
    26341 aaaagcgtcg gttttttcat ggctatactt agcgatgcac ggcagaactc gccgcgaaac
    26401 gtgacggtgg caacagatga atatttatgc ctttgatttt gatgagattg agagtcagga
    26461 ggatttttat cgtgacttta gccaaacctt tggtctggct aaagataagg tacgcgatct
    26521 cgactcacta tgggatgtgt taatgaacga tgtcctgccg ctaccacttg agattgaatt
    26581 tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc tgtttgatga
    26641 ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt cgtcattagc gcaaaaaaaa
    26701 gcccccgaac cgggggcaat atcgtcggac aagacgatga gggtttattt gtacagttca
    26761 gccgtagcgt gccaggtgtc accgctacga gcttcagtaa tctggtaggc cgttgcgcct
    26821 ttctcttccg cttttttgtt cagcatttca cgtatatcca ttggcgaaga cgccacacca
    26881 cttacggata cggtcccgat tgcttcacga ttttgtgctt gtgcagcatc aatggagtcg
    26941 gcagcgaatg caccgaaaga gagaacagaa agtacgctta atgcagctac agtggttttg
    27001 attttcatga tttttacctc gacataatct tttagctggg cctttgtttc gtgacccata
    27061 tcacaaaatc aagtatacac taattattga actaattaat accacgctaa ctgtttcagt
    27121 tgatactaag tgttaaaaat gtgaatttta ataacaaaaa agtataaaaa aacacggtta
    27181 tttattatta attatagtat tttcaattag ataatgaatt ttgcataatg tgcttttttt
    27241 accgtatgta ttcattttgt gaatgatgtg tcgcggtaaa acgcactatt cgttaaatgt
    27301 atcgcaggag aaagaagggg aatgagaggg ataagcaaca ttttccccgc cgccagaagc
    27361 gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt
    27421 tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca
    27481 gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag
    27541 ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca
    27601 atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt
    27661 tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat
    27721 taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt
    27781 tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc
    27841 gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta
    27901 tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca
    27961 aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat
    28021 taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt
    28081 tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg
    28141 gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg
    28201 taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa
    28261 tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag
    28321 gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat
    28381 atcgctccag tgactcccgg tgtggccgtc gatctgagcc atatccctac tgctgtgaaa
    28441 atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcattctt
    28501 atctctgcag gcgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac
    28561 gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt
    28621 ggtattatca ctaacccggt taacaccaca gttgcaattg ctgctgaagt gctgaaaaaa
    28681 gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc
    28741 aacacctttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt
    28801 ggcggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt
    28861 accgagcagg aagtggctga tctgaccaaa cgtatccaga acgcgggtac tgaggtggtt
    28921 gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt
    28981 ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt
    29041 gaaggcgacg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc
    29101 gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagaacgc gctggaaggt
    29161 atgctggata cgctcaagaa agatatcgcc ctgggcgaag agttcgttaa taagtaattg
    29221 attagcgaat aataaaaaac cggagcacag actccggttt ttgttttgag cactcgactt
    29281 aattggttgc cggatattcc tgaatggtga cctgcagcgt taactgctta tcatcacgca
    29341 tcactacaac cgggatcacc gaaccagggc gaatttccgc cacctgatcc atcgtctcca
    29401 gagcagagat ggccggtttg ttatccaccg aaataatcag atcgttgacc tgaatacccg
    29461 cattcgccgc tgggccgtca ggtgacactt cattaaccac gatcccttgc agttgatcta
    29521 taccaccgct ctgcgcgtgc agtggtgcga tctcgcgtcc gccgatacca atgtagccgc
    29581 ggatcacgcg accatcgcgg atcagcttat ccataatttt ggttgctaac tggaaaggaa
    29641 tcgcaaagcc gataccttcc ggcgtttcgc catcgttact cttatcaaac gacagcgtgt
    29701 taatgcccat cagttctccc agcgagttca ccagcgcgcc gccagagtta ccgtggttaa
    29761 tggaagcatc ggtttgtagg aagttttgcc gcccggtcgg gttcagaccg attcgacccg
    29821 tggcactaat aatcccttgg gtaatggtct gcccgaggtt gtacgggtta ccgatcgcca
    29881 gtactacgtc gccaatgtgc ggtacgcgac gtgcattaat tggaatggta ggtaaaccgc
    29941 cagtggcatt aattttaagt accgccagat cggttagaga gtcagatccc accagcaatg
    30001 cttcaaatac acgtccatcc tgtaaggcga cgatgatctg atcggcgtcg ttgatgacgt
    30061 gtttattggt gatgatataa ccgcgttgat ccatgattac accggatccc agggtgcgga
    30121 tctcaagctg gttgtgagag ttggtgttca aaccacggtt gtaaacgtta accaccgctg
    30181 gcgcggcgcg gcgaaccgcc agattatagc tggcaggcgt ctcatcggta ctgtcaaatt
    30241 gcggagtgga aagcgggtta aggctgcgca gcgaaggcat ggcaaccagc agaatagcgc
    30301 cgacaattaa tccaatcgca acggaacgta agagcttcac aaacatgatg gaggcgtcat
    30361 taaaaaaggg aacggcagca gcataccacg agttaaccgg acatcacacg tcagcctgat
    30421 gcccggttta cgacattaac gcatcagcag atagatgctt tcattgccgc gtacaatttg
    30481 cagggcgatg atggccggtt ttgccgccag cactttacgc atttcagcaa tcgagttcac
    30541 ccgatcgcgg ttgacgccaa tgatcacatc gtctttttgc aagccagcct gagcagctgg
    30601 gcttcctttg acaacttcat ctatcttaat acctttgccg ccatctttta gctgaccatc
    30661 gctcaacgtt gcaccttcca gcgctggcgt gatcatttca gcgctggccg acgaagaggt
    30721 gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca ggccaagctt
    30781 cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa agctattcag
    30841 cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt tcgctgagcc
    30901 agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt tgaaggcttt
    30961 ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt tgatttcacc
    31021 aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg caaatccaat
    31081 cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta actcaccgtt
    31141 aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg tctggataaa
    31201 gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa tgccagaggt
    31261 ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac cgacgcgcaa
    31321 tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa tttgtaacag
    31381 ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc gcccatcatt
    31441 gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca gcacatagcc
    31501 tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg caggttgatc
    31561 cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc cctgactggc
    31621 cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca ttggagccag
    31681 actggggaga ggggcctgat cggcaacctg gcctagaatc gacgcaacgg cctgaaatga
    31741 cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg tttgtttttt
    31801 cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag tttgatgtta
    31861 ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg ggagggagag
    31921 aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg cgccagtacg
    31981 cagcaggccg gatgcgcctt cagaatagtc gcgaggcatc tgcaccggtg cctgatcgtt
    32041 gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag acagttccgg
    32101 cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt cgtgcgccat
    32161 ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc gatactcgtc
    32221 cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc gtagtttacg
    32281 attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc caattagcgc
    32341 atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg ttggcttcag
    32401 gctccatgcc tacggctgat tatgccacta taaccgttaa ttccacagaa gtggaatccc
    32461 gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac aacggcgcac
    32521 cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat cgcaatacct
    32581 gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg ccgtcagccg
    32641 cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca ggacgagtgg
    32701 gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc atacgccagt
    32761 gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga tggacctttt
    32821 ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt ttatgctgcg
    32881 ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa ttattgccga
    32941 tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt ctgatattac
    33001 cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta ttaccctggt
    33061 agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac gtgcgcgttt
    33121 tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg acgctggtgt
    33181 tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac tcaacgatga
    33241 aacccgggcg caaatggata aactatggtt ggcgctggcg ggggtgaaac gagaaaattc
    33301 accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga accagacgct
    33361 ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact atattgcgct
    33421 ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc ggttgatgga
    33481 gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc atgtcaaatt
    33541 agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc tgaagtttga
    33601 gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc tgaagcgcga
    33661 gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt tgaccccgac
    33721 ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa ctttttgtgt
    33781 gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga acctcaacaa
    33841 ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct tttaatgaaa
    33901 acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga cgcgaccggt
    33961 aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa gcacaaagcg
    34021 gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc tgacaaagtt
    34081 gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg ccacatcggt
    34141 ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg tgtgattgaa
    34201 atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt ccgtaaactg
    34261 aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt tcttgacatc
    34321 taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg ccgcaaaagt
    34381 tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa ccaacgttct
    34441 ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc gctggaactg
    34501 gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg tatctctggt
    34561 caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga cgagtccctg
    34621 cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt tgaacgtaag
    34681 aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggcttct
    34741 gctccggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt atggctaaat
    34801 tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag acaggtaaaa
    34861 caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag cgcatctctt
    34921 tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa actatcatcc
    34981 aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga acaaagagag
    35041 tcgttcctta ttgggtaaca caacttctga ctggccacct ggccacctgg tggctggtag
    35101 cagtaaaaat tctgactata cctggaggtt ttcatggctg tcgctgccaa caaacgttcg
    35161 gtaatgacgc tgttttccgg tcctactgac atctatagcc atcaggtccg cattgtgctg
    35221 gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa aggacaatcc gcctcaggat
    35281 ctgattgacc tcaacccgaa tcagagcgtt ccgaccctgg tggatcgtga gctgaccctg
    35341 tgggaatctc gcatcattat ggaatatctg gatgagcgtt tcccgcatcc gccactgatg
    35401 cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca tgcatcgcat cgaaaaagac
    35461 tggtacacgc tgatgaacac tatcatcaac ggttcagctt ctgaagcaga tgccgcacgt
    35521 aagcaactgc gcgaagaact gctggcgatt gcgccggtct tcggtcagaa gccgtacttc
    35581 ctgagcgatg agttcagtct ggtcgattgc taccttgctc cgctgctgtg gcgtctgccg
    35641 caactgggca tcgagttcag cggcccgggt gcgaaagagc tgaaaggcta tatgacccgc
    35701 gtctttgagc gtgactcttt ccttgcttct ttaactgaag cagaacgtga aatgcgtctg
    35761 ggccggagtt aatctgtatg gatttgtcac agctaacacc acgtcgtccc tatctgctgc
    35821 gtgcattcta tgagtggttg ctggataacc agctcacgcc gcacctggtg gtggatgtga
    35881 cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga cgggcaaatc gtactcaaca
    35941 ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga tgaggtgcgc tttaacgcgc
    36001 gctttggtgg cattccgcgt caggtttctg tgccgctggc tgccgtgctg gctatctacg
    36061 cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc tgcctacgat gaagatacca
    36121 gcatcatgaa tgatgaagag gcatcggcag acaacgaaac cgttatgtcg gttattgatg
    36181 gcgacaagcc agatcacgat gatgacactc atcctgacga tgaacctccg cagccaccac
    36241 gcggtggtcg accggcatta cgcgttgtga agtaatacaa aacaggccca ggcggcctgt
    36301 tttgtctttt taatgactta ataa
//
LOCUS       DADKXM010000022        36165 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.022, whole genome shotgun sequence.
ACCESSION   DADKXM010000022 DADKXM010000000
VERSION     DADKXM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.022"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(37..2712)
                     /gene="adhE"
                     /locus_tag="JQR05_001154"
                     /gene_synonym="adhC"
     CDS             complement(37..2712)
                     /gene="adhE"
                     /locus_tag="JQR05_001154"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY5323367.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3189..3836
                     /gene="ychE"
                     /locus_tag="JQR05_001155"
     CDS             3189..3836
                     /gene="ychE"
                     /locus_tag="JQR05_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY5323368.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKFGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4574..6205
                     /gene="oppA"
                     /locus_tag="JQR05_001156"
     CDS             4574..6205
                     /gene="oppA"
                     /locus_tag="JQR05_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY5323369.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTAGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6291..7211
                     /gene="oppB"
                     /locus_tag="JQR05_001157"
     CDS             6291..7211
                     /gene="oppB"
                     /locus_tag="JQR05_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY5323370.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7226..8134
                     /gene="oppC"
                     /locus_tag="JQR05_001158"
     CDS             7226..8134
                     /gene="oppC"
                     /locus_tag="JQR05_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY5323371.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAISGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8146..9159
                     /gene="oppD"
                     /locus_tag="JQR05_001159"
     CDS             8146..9159
                     /gene="oppD"
                     /locus_tag="JQR05_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY5323372.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9156..10160
                     /gene="oppF"
                     /locus_tag="JQR05_001160"
     CDS             9156..10160
                     /gene="oppF"
                     /locus_tag="JQR05_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY5323373.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQEPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10213..10542)
                     /locus_tag="JQR05_001161"
     CDS             complement(10213..10542)
                     /locus_tag="JQR05_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY5323374.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10577..12037)
                     /gene="cls"
                     /locus_tag="JQR05_001162"
     CDS             complement(10577..12037)
                     /gene="cls"
                     /locus_tag="JQR05_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY5323375.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12180..12353
                     /locus_tag="JQR05_001163"
     CDS             12180..12353
                     /locus_tag="JQR05_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY5323376.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12411..13664)
                     /gene="kch"
                     /locus_tag="JQR05_001164"
     CDS             complement(12411..13664)
                     /gene="kch"
                     /locus_tag="JQR05_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY5323377.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13964..14260)
                     /locus_tag="JQR05_001165"
     CDS             complement(13964..14260)
                     /locus_tag="JQR05_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY5323378.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14475..15203
                     /gene="tonB"
                     /locus_tag="JQR05_001166"
     CDS             14475..15203
                     /gene="tonB"
                     /locus_tag="JQR05_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY5323379.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="JQR05_001167"
     CDS             complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="JQR05_001167"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY5323380.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15746..16285)
                     /locus_tag="JQR05_001168"
     CDS             complement(15746..16285)
                     /locus_tag="JQR05_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY5323381.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16315..17058)
                     /locus_tag="JQR05_001169"
     CDS             complement(16315..17058)
                     /locus_tag="JQR05_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY5323382.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIIAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(17086..17175)
                     /locus_tag="JQR05_001170"
                     /pseudo
     CDS             complement(17086..17175)
                     /locus_tag="JQR05_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17415..18053
                     /gene="ompW"
                     /locus_tag="JQR05_001171"
     CDS             17415..18053
                     /gene="ompW"
                     /locus_tag="JQR05_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY5323383.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18113..18619)
                     /locus_tag="JQR05_001172"
     CDS             complement(18113..18619)
                     /locus_tag="JQR05_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY5323384.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18666..19166)
                     /locus_tag="JQR05_001173"
     CDS             complement(18666..19166)
                     /locus_tag="JQR05_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY5323385.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19252..19431)
                     /locus_tag="JQR05_001174"
     CDS             complement(19252..19431)
                     /locus_tag="JQR05_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY5323386.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(19812..20618)
                     /gene="trpA"
                     /locus_tag="JQR05_001175"
     CDS             complement(19812..20618)
                     /gene="trpA"
                     /locus_tag="JQR05_001175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY5323387.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGIFAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(20618..21811)
                     /gene="trpB"
                     /locus_tag="JQR05_001176"
     CDS             complement(20618..21811)
                     /gene="trpB"
                     /locus_tag="JQR05_001176"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY5323388.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPSGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(21823..23181)
                     /gene="trpCF"
                     /locus_tag="JQR05_001177"
     CDS             complement(21823..23181)
                     /gene="trpCF"
                     /locus_tag="JQR05_001177"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY5323389.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(23185..24780)
                     /gene="trpD"
                     /locus_tag="JQR05_001178"
     CDS             complement(23185..24780)
                     /gene="trpD"
                     /locus_tag="JQR05_001178"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY5323390.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(24780..26342)
                     /gene="trpE"
                     /locus_tag="JQR05_001179"
     CDS             complement(24780..26342)
                     /gene="trpE"
                     /locus_tag="JQR05_001179"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY5323391.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(26434..26478)
                     /gene="trpL"
                     /locus_tag="JQR05_001180"
     CDS             complement(26434..26478)
                     /gene="trpL"
                     /locus_tag="JQR05_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY5323392.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26616..27497
                     /gene="yciV"
                     /locus_tag="JQR05_001181"
     CDS             26616..27497
                     /gene="yciV"
                     /locus_tag="JQR05_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY5323393.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27494..28114
                     /locus_tag="JQR05_001182"
     CDS             27494..28114
                     /locus_tag="JQR05_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY5323394.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28215..29087
                     /gene="rluB"
                     /locus_tag="JQR05_001183"
     CDS             28215..29087
                     /gene="rluB"
                     /locus_tag="JQR05_001183"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY5323395.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(29116..29706)
                     /gene="cobO"
                     /locus_tag="JQR05_001184"
     CDS             complement(29116..29706)
                     /gene="cobO"
                     /locus_tag="JQR05_001184"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY5323396.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACRKVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(29703..30461)
                     /locus_tag="JQR05_001185"
     CDS             complement(29703..30461)
                     /locus_tag="JQR05_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY5323397.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            30681..31730
                     /gene="sohB"
                     /locus_tag="JQR05_001186"
     CDS             30681..31730
                     /gene="sohB"
                     /locus_tag="JQR05_001186"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY5323398.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(31766..32017)
                     /locus_tag="JQR05_001187"
     CDS             complement(31766..32017)
                     /locus_tag="JQR05_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY5323399.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            32397..34994
                     /gene="topA"
                     /locus_tag="JQR05_001188"
     CDS             32397..34994
                     /gene="topA"
                     /locus_tag="JQR05_001188"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY5323400.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRDYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            35204..>36165
                     /gene="cysB"
                     /locus_tag="JQR05_001189"
     CDS             35204..>36165
                     /gene="cysB"
                     /locus_tag="JQR05_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY5323401.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKL"
BASE COUNT         9184 a         8753 c         9158 g         9070 t
ORIGIN      
        1 gaaggggccg tttatattgc cagacagcgc tactgattaa gcggattttt tcgctttttt
       61 ctcagcttta gccggagcgg cttctttttt cgctgcagtt tcaccttcta cataatcacg
      121 accgtagtag gtatccagca gaatctgttt cagctcggag atcagcgggt aacgcgggtt
      181 agcgccggtg cactggtcat cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa
      241 gtctgcttcc tgaacgccag cttcacggat agatttcgga atacccagtt cagctttcag
      301 cgtttccagc catgccagca gtttctcgat cttagcagca gtacggtcgc ccggtgcgct
      361 cagacccagg tggtcggcaa tttcagcata acgacggcga gcctgcggac ggtcgtactg
      421 gctgaatgca gtctgcttgg tcgggttgtc gttcgcattg tagcgaataa cgttacaaat
      481 cagcagggcg tttgccagac cgtgcggaat atggaactgg gaacccagtt tgtgcgccat
      541 tgagtgacat acacccagga aggcgttcgc aaatgcgata cccgcgatag ttgctgcact
      601 gtgaacacgt tcacgcgcta ccggattttt agacccttcg tggtaggacg ctggcagata
      661 ttctttcagc agtttcagtg cctgcagagc ctgaccatca gagaactcag atgccagtac
      721 agaaacataa gcttccatgg cgtgagttac tgcgtccaga ccaccgaaag cacacaggga
      781 cttcggcatg tccataacca ggttggcgtc gacaatcgcc atatccgggg tcagcgcata
      841 gtccgccagc gggtatttct gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac
      901 ttcagaacct gtaccagaag tggtggtgac agcgatcatt ttcgctttca cgcccatttt
      961 cgggaacttg tagatacgtt tacggatatc cataaagcgc agcgccagct cttcgaagtg
     1021 agtttccgga tgttcgtaca taacccacat gatcttcgcg gcgtccatcg gggaaccacc
     1081 acccagcgcg ataatcacgt ctggtttgaa ggagtttgcc agttctgcac ctttacgaac
     1141 gatgctcagg gtcgggtccg cttctacttc gaagaagact tcagtttcaa cgcctgctgc
     1201 tttcagtacg gaagtgatct gatcagcata accattgttg aacaggaagc ggtcagtcac
     1261 gatgagcgca cgtttgtggc catcagtaat cacttcatcc agcgcgattg gcagggagcc
     1321 acggcggaag tagatagatt tcggaagttt gtgccacaac atgttttcag ctcgcttagc
     1381 aacggttttc ttgttgatca ggtgtttcgg accaacgttt tcagagatgg agttaccacc
     1441 ccaggaacca caacccagag tcagggaagg tgcgagtttg aagttataca ggtcaccgat
     1501 accaccctga gaagccgggg tgttaatcag gatacgagct gttttcattt tctgaccgaa
     1561 gtaagaaacg cgagccggtt ggttatcctg gtcagtgtac aggcaagagg tatgaccgat
     1621 accgcccata gcaaccagtt tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg
     1681 gtacattgcc agagtcgggg acagtttttc atgtgcgaac ggttcgcttt catcaacaac
     1741 ggtcacttca ccgatcagaa tcttggtgtt ttctggtaca gagaagcctg ccagctcagc
     1801 aattttatag gctggctgac caacgatagc cgcgttcagc gcaccatttt tcaggataac
     1861 atcctgaaca gctttcagct ctttaccctg caacagatag ccgccgtggg ttgcgaaacg
     1921 ttcacgtact gcgtcataaa cagagtcaac aacaacaaca gactgttcag aagcacagat
     1981 tacgccgttg tcgaaggttt tggacatcag tacagatgca actgcacgtt tgatatcagc
     2041 agtttcatca ataacaactg gagtgttacc cgcgcctaca ccgatagctg gtttaccgga
     2101 gctgtatgcg gctttaacca tgcccggacc accagtcgcg aggatcaggt tgatgtctgg
     2161 gtggtgcatc agtgcgttag acagttcaac agaaggttga tcgatccagc cgatcagatc
     2221 tttcggagca ccggcagcga tagcagcctg cagaacgata tcagccgctt tgttggttgc
     2281 atcttttgca cgcgggtgcg gggagaagat aatggcgtta cgggtcttca ggctgatcag
     2341 cgatttgaag atagcagttg aagtcgggtt agtggtcgga acgataccgc aaataatacc
     2401 gattggttca gcgatagtga tggtaccaaa agtgtcgtct tcagacagaa caccacaggt
     2461 tttttcatct ttataggcgt tgtagatata ttcagaagca aagtggtttt tgatcacttt
     2521 atcttcgacg atacccatgc cggattcggc aacggccatt ttcgcgagtg ggattcgagc
     2581 atctgcagca gccagagcgg cggcgcggaa gattttgtct acttgctctt gagtgaaact
     2641 ggcatattca cgctgggctt tttttacacg ctctacgagt gcgttaagtt cagcgacatt
     2701 agtaacagcc ataatgctct cctgataatg ttaaactttt ttagtaaatc atctgctcga
     2761 atacgagagt atagtcagtg cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac
     2821 tcgcgctcac actcactgtg atttactaaa agagtttaaa cattagagtt attatctcta
     2881 atgcgtcact tccaggtggc gtaagcaaga ttactcactt ctgggtactg attacgtgat
     2941 ccaaatcaaa tttttgcaaa gctgatacct ttcagcatcg cttttcgcca ttatagctaa
     3001 cagttaataa attgtagtat gatttggtgg ctacattagc atgttttgca caactagata
     3061 acaataacga atgatagcaa ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg
     3121 tattgtcagc gcgtcttttc aaccttattt atggctaaca ttatccggct tttgcttcgg
     3181 agctaaccgt gattcagacc ctttttgatt ttcccgttta cttcaaattt ttcatcgggt
     3241 tatttgcgct ggtcaacccg gtagggatta ttcccgtctt tatcagcatg accagttatc
     3301 agacagcggc agcgcgaaac aaaactaacc ttacagccaa cctgtctgtg gccattatct
     3361 tgtggatctc gctttttctc ggcgacacga ttctacaact ttttggtata tcaattgatt
     3421 cgttccgtat cgccgggggc atcttggtgg tgacgatagc gatgtcgatg atcagcggca
     3481 agtttggcga ggataaacag aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta
     3541 ttggtgtggt gccattggcg ttgccgttga tggcggggcc aggggcgatc agttctacca
     3601 tcgtctgggg tacgcgttat cacagcatta gctatctgtt tggtttcttt gtggctattg
     3661 cattgttcgc tttatgttgc tggggattgt tccgcatggc accgtggctg gtacgggttt
     3721 tacgccagac cggcatcaac gtgattacgc gtattatggg gctattgctg atggcattgg
     3781 ggattgaatt tatcgttact ggtattaagg ggattttccc cggcctgctt aattaattcc
     3841 tttcaaatga aacggagctg ccatgctccg tttacttcgt cattattttt actttgttcc
     3901 cgcgcagtta tcaaaagcaa aaggaatagg taaaaatatt cttctcaaat tacagttagt
     3961 tataaggatt tccttaactg cttctcctca ccatcatgtt attttcgcca catcataatc
     4021 ctgggcttgc tgaagaataa ttgaaatgat attagtaatt ccactgcctt tggtagagga
     4081 aagtgctaaa taataatcaa ttgttaaatt attgtgcatt tcactactgg aactgtaatc
     4141 agaaaagata gacatgctta gccaatctct atttgattga attgaaagat gtttgttaag
     4201 gcatggatgc aagctataga ttctgatacg gtcaataaaa gagaatcgct taacaatttt
     4261 gcaaaatgta ttggcgagta agaaccgcat ttggtacttt ccgggcaacc gccagacgat
     4321 tctttattgg taatgagaat aattaacaat taaagagcgt cgcgaaagaa taatgtgtct
     4381 cgacagggga gacacagtac gaatcgacat aaggtgatcg tctgaatcac cagaataaat
     4441 aaagtcggtg atagtaatac gtaacgataa agtaacctga cagcagaaag tctccgagcc
     4501 tgtgcagggt cccaatccgg gattacacat gctggttaat accagtaatt ataatgaggg
     4561 agtccaaaaa acaatgacca acatcaccaa gagaagttta gtagcagctg gcgttctggc
     4621 tgcgctaatg gcagggaatg tcgcgctggc agctgatgta cccgcaggcg tcacactggc
     4681 ggaaaaacaa acactggtac gtaacaatgg ttcagaagtt cagtcattag atccgcacaa
     4741 aattgaaggt gttccggagt ctaatatcag ccgagacctg tttgaaggct tactggtcag
     4801 cgatcttgac ggtcatccag cacctggcgt cgctgaatct tgggataata aagacgcgaa
     4861 agtctggacc ttccatttgc gtaaagatgc gaaatggtct gatggcacgc cagtcacagc
     4921 acaagacttt gtgtatagct ggcaacgttc tgttgatccg aacactgctt ctccgtatgc
     4981 cagttatctg caatatgggc atatcgccgg tattgatgaa attcttgaag ggaaaaaacc
     5041 gattaccgat ctcggcgtga aagctattga tgatcacaca ttagaagtca ccttaagtga
     5101 acccgttccg tacttctata aattacttgt tcacccatca acttcaccgg tgccaaaagc
     5161 cgctatcgag aaattcggcg aaaaatggac ccagcctggt aatatcgtca ccaacggtgc
     5221 ctatacctta aaagattggg tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta
     5281 ctggaacaac gcgaaaaccg ttattaacca ggtaacctat ttgcctattg cttctgaagt
     5341 taccgatgtc aaccgctacc gtagtggtga aatcgacatg acgtataaca acatgccgat
     5401 cgaattgttc cagaagctga aaaaagagat cccggacgaa gttcacgttg atccatacct
     5461 gtgcacttac tattacgaaa ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg
     5521 taccgcactg aaactaggta tggaccgcga tatcattgtt aataaagtga aagcgcaggg
     5581 cgacatgccc gcctatggtt acactccacc gtatactgct ggcgcaaaat tgactcagcc
     5641 agaatggttt ggctggagcc aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga
     5701 agcgggttat accgcagaca aaccgttgac catcaacctg cggtataaca cctccgatct
     5761 gcataaaaag ctggcgattg ctgcctcttc attgtggaag aaaaacattg gtgtaaacgt
     5821 caaactggtt aaccaggagt ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga
     5881 tgtggcccgt gcaggctggt gtgcagacta caacgaacca acttccttcc tgaacaccat
     5941 gctgtcgaac agctcgatga ataccgcgca ttataagagc ccggcctttg acagcattat
     6001 ggcggaaacg ctgaaagtga ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga
     6061 acaacagctg gataaggatt cggccattgt tcctgtttat tactacgtga atgcgcgtct
     6121 ggtgaaaccg tgggttggtg gctataccgg caaagatccg ctggataata cctatacccg
     6181 gaatatgtac attgtgaagc actaatggca atacgtgggg caggagtgtc ctgctccacg
     6241 gtgtctgatt tttatcgcat tacagaaggc acaggccaga aggtagggca atgttaaaat
     6301 ttattctacg tcgctgtctg gaagcgattc cgacgctatt tattcttatt actatttcgt
     6361 tctttatgat gcgcctcgcg ccgggaagcc cttttaccgg cgaacgtact ttaccgccag
     6421 aagtgatggc caatatcgaa gcgaaatatc atcttaatga tccgatcatg acacagtatt
     6481 tcagctacct gaaacaactg gcgcatggcg atttcggtcc atcgtttaaa tataaagatt
     6541 attcggtcaa tgacctggtg gcatccagtt ttcccgtttc tgccaaactg ggagccgccg
     6601 catttttcct tgcggtaata ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa
     6661 acaccaaatg ggactatacc gtgatggggc tggcaatgac cggggttgtt atccccagtt
     6721 ttgtggttgc gccattatta gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg
     6781 gtggctggaa tggtggggcg cttaaattca tgatattgcc gatggtggcg ttgtcactcg
     6841 cctatatcgc cagtattgcg cgtattaccc gtggctctat gattgaagta ttacactcca
     6901 actttattcg tactgcccgg gcgaaagggt tacctatgcg gcggatcatt ttacgccacg
     6961 cattaaaacc tgctctgtta cccgtgctct cctatatggg ccccgcattt gtcggcatta
     7021 ttaccggttc tatggtcatc gaaaccattt atggtttgcc ggggattggg caactgttcg
     7081 ttaatggcgc attgaaccgt gactattcct tagtgttaag cctgaccatc ctggttggtg
     7141 cgttaaccat tttgtttaat gccattgtcg atgtgctata tgcggttatc gacccgaaaa
     7201 tccgttactg atactggagc tcgcgatgat gttaagtaag aaaaacagcg agacgctgga
     7261 aaatttcagt gaaaagctgg aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg
     7321 ttttatgcat aaccgtgcgg cggttgccag tctgatagtg ctggtgctga tcgcgttatt
     7381 tgtaatcctg gcaccgatgc tttcccagtt tgcctatgac gatactgact gggcgatgat
     7441 gtccagcgcc ccggatatgg agtccggtca ctactttggt actgactcat ccggtcgcga
     7501 cctgcttgtg cgcgttgcga ttagcgggcg tatctcactc atggtcggtg ttgctgcggc
     7561 actggtggca gtggtcgtgg ggacacttta tggttcgctt tccggttatc tgggcggtaa
     7621 agtggattcg gtaatgatgc gtctgctgga aatcctcaac tccttcccat tcatgttctt
     7681 cgtcattttg ctggtgacct ttttcggcca aaacatcctg ctgattttcg tggcgattgg
     7741 catggtttcc tggctggata tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg
     7801 caaagagttt attgaggcgg cacaagttgg cggtgtatcg acgccgggca ttgttattcg
     7861 ccacattgtg ccgaacgtac tcggtgtggt ggtggtctac gcatcgctac tggtgcccag
     7921 catgatcctc tttgaatctt tccttagctt cctggggttg ggtacgcaag agccgttaag
     7981 cagctggggg gcattgctga gtgatggcgc gaactcgatg gaagtctctc catggttact
     8041 gttgttccca gcgggattcc ttgtggtgac actgttttgt ttcaacttta tcggcgatgg
     8101 cttgcgtgat gccctcgacc cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa
     8161 actgcaactg tgccgctcgc acaacaacag gctgacgcac tgctgaacgt gaaagatttg
     8221 cgtgtcacct ttagtacccc ggacggcgac gtcacggcgg tcaatgattt aaatttttcc
     8281 ctacgtgccg gagaaacgct gggcattgta ggtgagtctg gttcgggtaa atcgcaaact
     8341 gcatttgcgt tgatgggcct gttggctgcc aacggacgca ttggcggatc ggcaaccttc
     8401 aatgggcgtg aaatcctcaa tttgccagag cgtgaactca ataaactgcg cgctgaacaa
     8461 atctcaatga tttttcagga cccaatgact tcgttgaatc cctatatgcg cgtcggtgag
     8521 cagttgatgg aagtgctgat gctgcataag aacatgagca aagctgaggc gtttgaagag
     8581 tcggtgcgga tgctcgatgc ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac
     8641 ccgcacgaat tttctggcgg catgcgtcag cgagtcatga ttgcgatggc attgctatgt
     8701 cgacctaagt tgctgattgc ggatgaaccc actacagcgc tggacgtcac cgtacaggcg
     8761 cagatcatga cgctattgaa tgaactgaag cgggaattta atactgccat cattatgatc
     8821 acccacgatc ttggcgtggt ggcggggatc tgtgacaaag tgctggtaat gtacgccggg
     8881 cgcacgatgg aatatggcaa tgcgcgcgat gtcttttatc aacccgttca tccttattct
     8941 atcggtttgc tcaacgcggt gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc
     9001 ccgggtaatc cgccaaactt gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt
     9061 ccacatgcga tggaaatttg tagtagcgca ccgccgctgg aagagtttac gcctggccgt
     9121 ctgcgtgctt gctttaaacc ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa
     9181 aagtcctcct tgaaatcgcc gatcttaaag tgcactttga aatcaaagat ggcaaacagt
     9241 ggttctggca accgccgaaa acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg
     9301 aaggggaaac attaggtgtg gtaggggaat cgggatgcgg taagtccacc tttgctcgcg
     9361 ccatcatcgg tttggtcaag gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc
     9421 tgggcatgaa gcccgatgaa tggcgtgccg ttcgcagtga tattcagatg attttccagg
     9481 agccgttggc atcgctaaac ccgcgtatga ccatcggcga gatcatcgct gaaccactgc
     9541 gtacttatca tccgaaaatg tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc
     9601 tgaaagtcgg gttattgcct aacctgatta accgctatcc gcatgagttc tccggtgggc
     9661 agtgccagcg tatcgggatt gctcgtgctc ttattcttga accgaagctg attatctgcg
     9721 atgagccggt ttcggcgctg gacgtgtcaa ttcaggcgca ggtggtcaac ctgctccagc
     9781 agctgcaacg tgagatggga ttgtcattaa tttttattgc tcatgacttg gccgtggtaa
     9841 aacacatttc cgatcgtgtg ttggtgatgt atctcggcca tgcagtagaa ctggggacct
     9901 atgatgaggt ctaccacaat ccactacatc cttacaccag ggcattgatg tcggcagtcc
     9961 ccatacctga tccggatctg gagaagaaca aaaccatcca gttactggaa ggggaattac
    10021 cgtcgccgat caacccgcct tccggttgtg ttttccgtac ccgttgcccg attgccggtc
    10081 cggagtgcgc caaaacacgt cctgttctgg aggggagttt cagacacgcc gtttcttgcc
    10141 tgaaagtcga tccgctttaa aaacaataag ggctgacagt tgtcagccct ttttcacgct
    10201 aaaagcgatt atttattccc gccagatgat atggcaaagt ttgtgatctt tttcgcggca
    10261 taacagaatg cgggcgaaaa catcgttgat ttcaccatct tcactgtccg ccagaccaat
    10321 caccacttcg gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct gccagtcttc
    10381 cgccggatcg aataactccg cgccgccgcg ctcttcaaac tgaagattga acagcagaac
    10441 atcagctgga tcgagattgt cggcagccag ttcgagaaaa atatcgtagg cctgctcaag
    10501 cgtttcatct tcagtcaggc gattgtttag atccatatcc atagtcacta cctgtttaac
    10561 ctctgttggc gacgttttac agcaacgggc tgaagaagta aaacagtcgc tcggcgacac
    10621 gttgccataa tggacgtttt agccataaac gggcatcgag cagacgtgaa cgcgaaatat
    10681 aatcgtcctg aacggcggca aggtctgcac caaaaccttt atcgtcgatt gccagggtaa
    10741 tctcgaaatt cagccacaga ctacgcatat caaggttaac ggtgccaacc agacttagtt
    10801 cgccatcgac cagcacgctc ttggtatgca gtaacccgcc ttcaaactga taaattttaa
    10861 ccccagcagc aagcagttcc gtaaagaatg cgcgactggc ccagccgacc agcatcgagt
    10921 cattttttcg cggaaggata atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg
    10981 catgaagtaa atcatcgctt ggcacaaagt agggcgtggt catgatcaaa tattcacgcg
    11041 ccgaataagc cgcagtcaat aatgcctggt gaatgagatc ttccggaaag ccggggccag
    11101 aagcaattgt gtgaatggtg tgaccgctgg cctgttcaaa cggcataata ttgacatctg
    11161 gtggtggcgg cagaatacgt tttccggttt caatctccca gtcgcaggaa taaataatcc
    11221 ccatcgcggt ggcgatgggg ccttccatac gcgccatcag atcaatccat tgccctacgc
    11281 ccgcatcttg tttgaagtag cgaggatcga ccatattcat gctgccggtg tacgcgatgt
    11341 aattatcgat catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac
    11401 gcatcagatt gacctttaag gcttcgacca cttcaatacc ggcattacgc attagctcgg
    11461 gccacgggct gcggaaaaaa gccacactcc cggcggagtc gagcatcaat cggcaatgaa
    11521 taccacgccg tgcagccgcc attaatgatt cagccacctg gtccgccatg ccgccgggct
    11581 gccagatata aaacaccatc tcaatattat ggcgcgcgag ctggatgtcg cggattaacg
    11641 cctgcatcac atcatctgac tcggtcatca gttgtagctg attccctttg accccagcga
    11701 tcccctgacg acgctcgcaa agcttgaata atggcgcagc gacactgcta ttttcttcgg
    11761 cgaagatatg cttacaggct ttaaggtcgt taagccattt tgcggtggaa ggccacatcg
    11821 ctctggcgcg ctcagcgcgg cgtttgccta aatggagctc gccaacggca agataggcaa
    11881 taattccgac taacggcaga atgtaaataa tcaacagcca ggccatcgcg gagggaactg
    11941 cgcgtcgttt cattagaatg cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa
    12001 tggccaacca actcaccaac gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc
    12061 gcattgttat gagtttacgc atatggattc atctggcaaa taaaaacgcg ggaaaagcac
    12121 tggtcagcgt cgggggcggg ggtataatga ccattctgtt attgcataga gtagttaaca
    12181 tgaagcggag tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct agccgacgta
    12241 aatcgcgttg gcttgagggg caatcgcgcc gaaatatgcg tatccacagc atcaggaagt
    12301 gcattctaaa caaacagcgt aactcgttat tgtttgcgat ctacaatatc taaatgtact
    12361 gggcaccatt attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
    12421 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctaaa aataccatga
    12481 cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
    12541 cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg atgtaccatc
    12601 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
    12661 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
    12721 atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt actgtcaccg
    12781 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
    12841 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
    12901 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
    12961 ccttttaaaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
    13021 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
    13081 gaaacaggga caatatctcc atagccgacg gttgacatgg tttctatcga aaaatagaac
    13141 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
    13201 gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg
    13261 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
    13321 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
    13381 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
    13441 acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccaactct
    13501 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
    13561 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
    13621 cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
    13681 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
    13741 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
    13801 tgcgtcagga aagggctttt ttcctgaaca tgctcacatc atataaagaa atacagataa
    13861 agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag
    13921 aacacggttg caaaaaccgt gcccttaaat attggatctc tactcagaac actttcttaa
    13981 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
    14041 aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca gtaaatcccg
    14101 cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac aaacgacctt
    14161 catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga
    14221 ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact ccttgttcgg
    14281 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
    14341 tgttaaggtc atgcataaag taagggtaat tacgccaaaa atgacatttt cactgatcct
    14401 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgagg cttggttttt
    14461 ctactgaaat gattatgact tcaataaccc ttgatttacc tcgccgcttc ccctggccga
    14521 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat acctcggtac
    14581 atcaggttat tgaactacct gcgcctgcgc aaccaatttc tgtcacgatg gttgcgcctg
    14641 ctgatctcga accgccacaa gccgttcagc cgccaccgga gccggtggta gagccagaac
    14701 cggaacctga gccgatcccc gaaccgccaa aagaagcacc ggtggtcatt gaaaagccga
    14761 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt acaggagcag ccaaaacgcg
    14821 atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcgcgcc
    14881 ccacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac
    14941 cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg
    15001 aagggcaggt taaagttaaa tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa
    15061 tcctctcagc caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg atgcgcagat
    15121 ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta
    15181 acggcaccac cgaaattcag taagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
    15241 tattactcaa caggtaaggc gcgaggtttt ccttcaggat caaccgcgac atacttaaat
    15301 aatgcttctg tcgctttata gcgttgcccg attggttcag acgctacttt tttcacccac
    15361 acttcaatat taatgctgac cgatgtcgtc cctttctgga cacagcgtgc atagcagcac
    15421 accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacagtcact
    15481 acgcgaccgt gggcaatttc ttttgccaga atagcgccgc caatatccat ttgtgacatt
    15541 aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt
    15601 aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc gacttaaaag
    15661 gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc
    15721 cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga
    15781 tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa
    15841 agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa
    15901 ggataaagaa aacagcccag gccagattta gcttcgacca taccggttgc ggcagcgtga
    15961 gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg ctgactaaca
    16021 gggctcccgc aaacagagca taaatgactg taaccttcca tttaataaac tcatcattgt
    16081 ggaagaacag cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct
    16141 tctcaacctt acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca
    16201 gcgctgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg
    16261 gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt aaggttattg
    16321 gcgaatcaac atatacaggc ggaacagata gatgagcaat atggcagaaa tcaggttgct
    16381 caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa
    16441 aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat
    16501 attcgcccaa gtcagccgca tactgctacg catcgaggca aaaatgccca ttttgtcctg
    16561 taccagcatc accggtgcca gggccagtaa aatggctatg ataattcccg gaacgaccac
    16621 cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataacttggg
    16681 caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac
    16741 cagctggata attaatatta cgcctccggc gagaatggcg ttaccgatta attctgaaaa
    16801 agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac
    16861 caggtcgaac aacccactac tgccgctaac gggcacgccg tcattgagct gcgcaagctg
    16921 tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga
    16981 taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga
    17041 ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa
    17101 ttgtaccctg gttaagcctc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt
    17161 gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag tagcgatcga
    17221 gttaatattt gcgtagagaa aatatttaaa aattgattta aatcacatta accaggattc
    17281 tcaatgcaac ttctaaatta atccagatca ataaatggtg aattatcata tgtaatgtga
    17341 tctatgtagg atcatttgtt actccaatgt aggtatattc gtcacgtttt tataaccata
    17401 acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtaaca actcttcttt
    17461 ctggcagtgc ctttgcgcat gaagcaggcg aattttttat gcgtgcaggt tctgcaaccg
    17521 tacgtccaac agaaggtgct ggtggtacgt taggaagtct gggtggattc agcgtgacca
    17581 ataacactca actgggcttg acgtttactt atatggcgac cgacaacatt ggtgtggaat
    17641 tactggcagc gacgccgttc cgccataaaa tcggcacccg ggcgaccggc gatattgcaa
    17701 ccgttcacca tctgccgcca acactgatgg cgcagtggta ttttggtgat gccagcagca
    17761 aattccgtcc ttacgttggg gccggtatta actacaccac cttctttgat aatggattta
    17821 acgatcatgg caaagaggcg gggctttccg atctcagtct gaaagattcc tggggagctg
    17881 ccgggcaggt gggggttgat tatctgatta accgtgactg gttggttaac atgtcagtgt
    17941 ggtacatgga tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata
    18001 gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca
    18061 caaaaacgac cccgtaatat acggggtcaa taaggacatg gtataaaggg tattatttct
    18121 tcgcttctac gccatcagtt tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat
    18181 gctgaatcag ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa
    18241 ttgagactgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt
    18301 caaattgctc gaagacatat ccgctaatag agcctttaac tatttcatca gaagggaata
    18361 taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac
    18421 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
    18481 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacgg ctggccatgg
    18541 attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
    18601 aatgttcaat acgattcatg aaaatgtcct catttataaa atgactaaaa gttaaaattc
    18661 atatttcagg ctttattttc ggctttctta tttacgttat taagggccag atcagtcagt
    18721 ttgatgtcgg tggccttttc ttcttccagg gtttctttca gaagcttcgc tgctttacgg
    18781 taacctaatt gttcagccag cgtcgctaat gtcccgtaac tggcaatctc ataatgctcg
    18841 actttctgtg ctgcggcaat cagtgcggca tcacgcactt cgtttttctc ggtactttcg
    18901 atgacctcat tagcttcttc aataagacct tccattgcca cacatttcat gcgcttaatt
    18961 ttcagatttg attctgattc cacaacttga tcaattcgtt caatctgtcc atgagtttcc
    19021 tcgaggtgcg catgaaaagc ctgacttaat ttttcatttg atgttgctct tgcgagtttt
    19081 gccagtgccc gggttaattg tttttctgcg ctgtaggtat ctgaaagcag ttgaataaat
    19141 acatcttcaa tggtcttcat attcatattt ttctccagtg aaatcactgc gagccatacg
    19201 gcccgcagat gcaattatct ttatcagctg atattaataa ttaacggtga atcaggattt
    19261 gcctgattta ttaccaccgc tttgttgacc gcctttttta cccgcttcag atgcgcgttg
    19321 cggatcattt ttaaaattac cgccgctatg ctgaccgcct ttacggcctg cgtcggatgc
    19381 cttctcacgg tcttcggcga aatttcctga accaccacga tgttcggcca tgttatttct
    19441 cccgttgcgt tgcattgttt cattaatatg agtgttgtgt gtcgacactc attaaaatta
    19501 gtcgataatg agaattagtc aaattaagcg caacgagaag atagagggaa aatatatttt
    19561 gaggaacatt ctggatatat taacaattac ctgaggaata agtgacttag agaaaattat
    19621 tgattttact ggtgttatgt tgcgggatta atttgtttga ttatattact gttgggcgga
    19681 aaaatgacgt aagttgacgt tcgaccgggg taagcgaaac ggtaaaaaga taaatattaa
    19741 atgaatttag gatttttccg gcttcattaa agaaagttaa aatgccgcca gcggaactgg
    19801 cggctgtggg attaactgcg cgtcgccgct ttcatcggtt gtacaaaaac tttcagtgcc
    19861 gccagcattt tctctggctc attaatatgt tgctcgatga ttttaacaat ggccgaacca
    19921 gaaatcgcgc ccgcagctcc tgcatcaatc gctgctttta cctgatccgg ggcgaaaata
    19981 ccaaacccct gcaatggagg tgcagcgttg tactctttca gcttcgcaac cagatgattg
    20041 aggggtaacg cggcgcggtt ttctgcgccg gtcacgcccg ctcgtgacag caaatatgtg
    20101 taaccacgac cgtaagaggc tatctggcgc agcaggtcgt catcggcatt tggcgggcag
    20161 atgaagatag gtgcgacatt atggcgcaac gcggcctggc ggaagggcgc ggactcttca
    20221 actggcacat cggcaaccag caccgaatcg acgccgactt tctcgcactg ggcataaaac
    20281 tcatcaatgc ctttgttaaa caccagattg gcatacatca acaggccaat gggaatggtc
    20341 gggtgtttct ggcgaatcag tgccagcatt tcaaaacatt gtgccggagt cacacctgcc
    20401 gcaaaggcgc gcagagtggc gttttgaatg gtcgggccat ccgccagtgg gtcggagaag
    20461 gggataccta actccagcgc gtcagcaccg gcttcaatta gcgtatcgat aattttcaat
    20521 gactgctcaa tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc ttctttgcgc
    20581 tccttcaact gggcaaacag agattcgtag cgttccatca gatttcccct cgtgctttca
    20641 aaatatcgtg aacggtgaag atgtctttat cgccgcgacc ggaaaggtta accaccagta
    20701 gctgctcttt atccgggttt tcgcgcatca ttttcagcgc atgggccagg gcgtgggagg
    20761 attccagcgc cgggatgatc ccttcgtgca ggcacagcgt tttgaaggct tcaagggctt
    20821 catcatcggt aatagacacg taatcagcgc gtccagtgct gttaagatac gcgtgttgtg
    20881 ggccgacaga cgggaaatcc agtccggcgg agatggagta agattcttca atctgcccgt
    20941 cttcggtttg catcatcggc gctttcatac cgaaataaat gcccacgcga ccatgtttta
    21001 gcggtgcgcc gtgctcgcca gtttcgatac cgtgaccact aggctccaca ccaatcaggc
    21061 cgacgttggt ttcattgatg aaatcagcaa acatgccgat ggcattcgaa ccgccgccaa
    21121 cacaggcgat aacggcatcc ggcaggcgac cttctctttc cagaatctgc gctttggttt
    21181 cttcgccaat catccgctga aactcacgga caatggtcgg ataaggatgc gggccagccg
    21241 cggtgcccag catatagtgc gcggtttcat aactgccgga ccagtcgcgt agcgcctcat
    21301 tacaggcatc tttcagcgtg gcggaaccgc tatgtaccgg gatcacttcc gcacccatta
    21361 agcgcatacg aaaaacgtta ggcgactggc gttcaacgtc tttggcaccc atataaatac
    21421 ggcatttcag gccgagcagg gcactggcaa gggccgacgc cacgccatgc tgaccggccc
    21481 cggtttcggc gatgatttcg gttttaccca tccgcttcgc cagcaacgcc tgccccagca
    21541 cctggttggt tttatgtgcg ccgccgtgca gcaaatcttc acgcttgaga tacagcgtgg
    21601 tgttcgtccc ggctgtaatg ttctggcatt tggtcagtgc ggttggacgc ccggcatagt
    21661 ttttcagcag gtcgttgaac tgggcttgaa attcaggatc tttttgcgcg ctgacaaaag
    21721 cttcttccag ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg ccgccaaact
    21781 caccaaaata ggggttaagt aatgttgtca ttgttccttt ccttaatatg cgcgcagcgt
    21841 ctggaaaacc gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact ctacagcaga
    21901 attaaaatca agtccggcgc agccggtttg tgccgcttcc acgcagttat ctgcgcctaa
    21961 gccccccgcc agcagaacgt tgccaagcga ttgaccattt aatagtgacc agtcaaaacg
    22021 ttgtccgctt ccaccctggc cgttgtctaa aacatattta tcaacgtgct gaaactcgcg
    22081 ggcgggcagg gtttcaccga cgcttaatgc tttccagatg gcaacatgtg ctggcagagc
    22141 ttcacgcagc gtatcgatat acagctgatc ttcattacca tgcagttgca ctgccgccag
    22201 cgataacacc ttagctttgt ccaccacatc ggcaatatcg tgattgcgga acacgccaac
    22261 atactgcaac ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga cgcaacgcgg
    22321 tgatgtcgca acaaaaatca acccaccgta aatcgcgccc gcgtcataag ctgctttagc
    22381 atcttgccca cgcgtcaggc cacatacttt attctcaccc agcaacaccc ggcgcacggc
    22441 ggcgtgcaaa tcgtcatggg ccatcaacgc cgaaccaatc agaaaaccgt tagcgaagtg
    22501 gcttaactcg cgcacctgag cgtaagtatt gatgccggat tcgctgatta ccgttacgtt
    22561 gtgccccagt ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca aatcacgcag
    22621 atcgcggttg ttgatgccaa cgacctttgc ccctaatgca atggcgcgct ccagttcctc
    22681 ttcattactg acttcggtca gcacccccat ctccagactg tgagcgacgg ctgcaagctg
    22741 gcgatattgg tcgtcatcca gtactgaaag cattaataag caggcatcgg cctggtaata
    22801 gcgcgccaga tagatctggt aagggtcgat aatgaagtct ttacataaaa tcggctgcgg
    22861 ggcgatttgg ctgacgatgg ggaggaaatc aaagctcccc tgaaaatatt tctcatcagt
    22921 cagcaccgaa attgccgaag cgtaatgttt ataaatggcg gcaatgcgtg ccggatcgaa
    22981 atcatcacgg atcacgcctt ttgacggcga cgctttttta cactccagaa taaacgccgt
    23041 gcgtgcaccc tgtagcgcat cataaaaatg tcgtgtgctc ggctgaacct cattctgaaa
    23101 actggccagc ggttgctgct gtttgcgggt ttctacccaa atcgccttgt ctgcgacgat
    23161 tttcgctaaa acggtttgca tcatttaccc tcgtgccgcc agcgcggtaa ctctgtcgta
    23221 agcggaacca ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca gatcttcatg
    23281 gccatgcagg cgcattaaca tggcgacgtt cgcagcgacg gctgcttcat gggcggcgtc
    23341 gcctttacct tgtaacaagc gtgttaaaat gtcacggttt tcttcaggtg ttccgcctgc
    23401 cagttgctcc tggtggtagg gagtcaggcc aaaatcttca gcggtcaatt gatagctctt
    23461 aattttgccg tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa cttcatccat
    23521 cccgccgctg tgcaccaccg ccgcgcgttg ataccccagc acgcgcaagg tttcggcaat
    23581 cggcagcacc agttccggac tataaacacc aattaacgcc agcggcggat gcgccgggtt
    23641 aatcaatggc cccagcacat tgaacagggt gcgggttttc agttgctggc gaaccggcat
    23701 cgcgtggcgg aatccggtgt gatatttcgg cgcaaagagg aaacatacgc ctaactcgtc
    23761 cagcgcctgg cgcgatttat tggcgttcat atcaagatta ataccgaatg ccgccagcag
    23821 atccgacgag ccggatttac tggagacgct gcggttaccg tgtttcgcca ctttcagccc
    23881 acaggccgcg gcgacaaacg cactggcggt agaaatattg atactgttgc tgccgtcacc
    23941 gccagtaccg acgatatcag caaacagata atccgggcgc gggaacggcg ctgcgttttc
    24001 cagtagcgcg gttgctgccc cggcgatctc gttcgggtgc tcaccgcgaa ttttcatgct
    24061 caccagcgcc gccgccagtt gttccggctt cagctcgcca cgcaccaccg ctgaaaacag
    24121 ctggtggctt tcttgttggc taagcgtctg cgcctgatac agtttttcca gaatcggttg
    24181 cagcgtgttg gctggctcta gtttctgctg cgcccaggcc agcgtttgtt ccagcaggcg
    24241 agcgccctgg gtggtgagaa tggattccgg atggaactgg aatccacaaa cgcgatccgc
    24301 atcgtgacgt actgccatca ccatgccatt aaaatgggcg ttgatggtta aaccggccgg
    24361 aatgttactg ccaaccagcg agtgataacg cgccaccggc agcgggtttg ttaatccggc
    24421 aaacatcgcc tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa tttcgcccgc
    24481 ctgaccgaca tagcccccgt aagcttcgac aattgcctga tgtccgaggc aaatgccaat
    24541 aatgggcagc ttgccacgca agcgggtgag gagttccggc atacaaccgg cttcgctcgg
    24601 cacaccgggg ccaggagaaa gcatcagtac cggattgctc atcgtcgcca ggcgttcaat
    24661 taaggtctgc gccggaatat ggttgcggta aatcaccacg ttatgaccat tgctgcgcaa
    24721 ctgatctgcc aggttgtacg taaaagagtc gatattatcg agcagcagaa tgtcagccat
    24781 cagaaagtct cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc gcgggctttg
    24841 ttacgggttt cgtcggcttc cgactgcgga acagaatcaa ggactacacc agcacccgct
    24901 tgcacggtgg cgataccgtt ttccaccagc gccgagcgga tcacaatgca ggtgtcgaga
    24961 tcgccatgcg cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg acgaccttcc
    25021 gcctcggcaa ttaactgcat agcgcgtact ttcggcgcac cgcttaacgt ccccatattc
    25081 atacaggcgc gataagcgtg cagggcgtca agatcgtgac gcagttcgcc gactacgcga
    25141 gagacgaggt gcatcacata ggaataacgg tcaactttgg tgagatcggc gacgtagcgg
    25201 ctgccagggg tgcaaatgcg tgccagatca ttacgggcga gatcaaccag catcagatgt
    25261 tcagacagct ctttatggtc agtacgcatt tccagttcaa tacggctgtc gagatctctg
    25321 tccagtgaac catcggcgcg acgaccgcgt gggcgtgttc cggcaatcgg gtagatctca
    25381 atctggcggc tggtggcatc atacttgagc gagctttccg gcgacgcgcc aaatagggtg
    25441 aaatcattat cctgcataaa aaacatgtac gggctgggat tactcttttt cagcacgtaa
    25501 taggccgcca gcggtgacgg gcagggcaga gagaaacggc gagatggcac cacctggaaa
    25561 atttctccag cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa ctcttcatcg
    25621 ctctgattac attcacaacg catatgcggc acggaaacca ctggcagcgg cggcgcggct
    25681 tcggtcagtt gctgacgtag ttcgttcagg cgagcagtga gacgttgttt ttcttcttca
    25741 ttcggagcaa acaggctggc ctgaatacgg gtgctttttt tctgatggtc aatcaccatc
    25801 agcgtttcag cgagataaaa acagaaatca gggcagttat tttccgctga cagttgcggt
    25861 aaatcttcaa atcccgccac aaggtcataa gagaacaggc cgccgaagaa cattgcttct
    25921 cgttcttcct tcggtacatt caacagattc tgcaataaac ggaaagcgtc aaaaaccgaa
    25981 agggagcata agcgggcgtc ttcatccagc agtggactga caggggggaa gcgcagcaca
    26041 cggcagtttg gtgattgttc actttccaca cccgcaggca gggcgttatc cagtagtgcc
    26101 agcagggctt cgccattgcc ggaaagtgcc tgaattgtga cggtgtcacc taatgctgta
    26161 atgcgcagcg cactgtctat cagcagcagg ctttttaaat catctttgct gtcgatatct
    26221 gcggattcca gcagcagcgt tgccggacga ttcccacaca actggtgaaa aagcgcagtc
    26281 gggttgtcgc gataagcgcc ttcgcaggtt agcagttcga gagtcggttt ttgtgtttgc
    26341 attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg
    26401 attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc aacctttcag
    26461 tacgaaaatt gctttcattg tcgataccct ttttacgtga acttgcgtac tagttaactg
    26521 gttcgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc
    26581 ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga
    26641 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg
    26701 tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat gacaccacag
    26761 ctgcaatcgc gccagcaaga gaggaaattt cacgttcggg actggcgctg aatcttattc
    26821 ccggcgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc gggctgaata
    26881 ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc
    26941 agcgggcgca gcaaattgcc gaaagacttg aaaaagcgca aatacctggg gcgctggaag
    27001 gcgcacaacg actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg cgtttccttg
    27061 tggagtgcgg caaagccagt tcaatggcag atgtctttaa aaagtatctg gcgcgcggga
    27121 aaaccggata cgttccgcca cagtggtgta caatagaaca agctattgat gtcattcatc
    27181 attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt gctaaatggc
    27241 tgaaaagatt ggttgcgcat tttgccgaac accacggcga cgcgatggaa gtcgcgcagt
    27301 gccagcaatc gcccaatgaa cgcacccagc tggccacact tgcgcgtcag catcatttat
    27361 gggcatcaca aggatctgat tttcatcagc catgcccgtg gatcgaactg ggtcgtaaac
    27421 tctggctgcc cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg cagaacacca
    27481 cagagaggga attatgagcc agttttttta tattcatcct gataacccgc agcaacgtct
    27541 gatcaaccag gcggtggaga tcgtgcgtaa aggcggggtg attgtctatc caactgattc
    27601 cggctatgcg ctcggctgta aaattgaaga taaaaacgcc atggagcgta tttgtcgtat
    27661 tcgccagctg ccggacggtc acaactttac cctgatgtgt cgcgatcttt ctgaactctc
    27721 gacctattca ttcgtggata atgttgcgtt tcgtttgatg aaaaacaaca cgccgggcaa
    27781 ctataccttc atcctgaagg ggacgaagga agtgccacgc cgtctgttgc aggagaaacg
    27841 caaaaccatc ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc ttgaagcact
    27901 gggcgaaccg atgctttcca cttcgctaat gctgccaggc agcgaattta ccgaatccga
    27961 tcccgaagaa attaaagacc gtctggaaaa acaggtagat ttgattattc acggcggcta
    28021 tctcgggcag aaaccaacaa cggttattga tcttaccgat gatacgccgg tcgtggtgcg
    28081 tgaaggcgta ggtgatgtga agcctttctt ataagtgcgt aactctgtat actacgcggc
    28141 cttaaaatgg cgctatacgg cgccattgat tctcctgttc gacgcctgtg aaggcgacac
    28201 caaaaggaag ctctatgagc gaaaaattac agaaagtgct ggcccgcgct ggccacggct
    28261 cccgccgtga aatcgaatct attattgaag ccggtcgtgt gagtgttgat ggcaaaattg
    28321 ctaaactcgg cgatcgcgtt gaagttaccc ctgggctgaa aattcgtatc gatggtcatc
    28381 tgatttcggt acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg
    28441 aaggtgagtt gtgtacccgt aacgatccgg aagggcgtcc gacggtgttt gaccgtctgc
    28501 caaagctgcg tggcgcacgc tggattgccg taggtcgtct ggacgttaac acctgtggtc
    28561 tgctgctgtt caccaccgat ggtgaactgg caaaccgttt aatgcatcca agccgtgaag
    28621 ttgaacgtga atacgccgtg cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt
    28681 tgagtcgtgg cgtgcaactg gaagatggtc cggcagcttt taaaaccatc aagttcagcg
    28741 gcggcgaagg gatcaaccag tggtacaacg tgactctgac cgaaggccgt aaccgtgaag
    28801 ttcgtcgtct gtgggaagcg gttggtgtgc aggtgagccg cctgatccgt gttcgttacg
    28861 gtgatatccc actgccaaaa ggtctgccac gcggtggctg gacggagctg gacctcgccc
    28921 agactaacta tctgcgcgaa ctggtggagc ttccgccaga aaccagctct aaagtcgctg
    28981 tagaaaaaga ccgtcgtcgc atgaaggcta atcagattcg tcgtgcggtg aaacgtcaca
    29041 gtcaggtgag cggcggtcgt cgttctggcg ggcgtaataa cggttaatca aagtatgccg
    29101 gacgtcatat ccggattaat aatcaatccc tatttgcgcc ttaacaccgg catcaaacgc
    29161 atgtctgatg gggcgcagct cacttaccgt atccgccagt tcaagaatat cccgatgaca
    29221 gcctcgaccc gtgataatca ccgtctgttg atgtggacgt tcatttaacg cctgcaccac
    29281 ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcaa gcaaaaccat
    29341 atccagtgag gaatcagcaa gcatccgctt tgcatgttgc cagactttgc ggcaggcggc
    29401 ggtatcagac tcgcggtttt gtgtatccca ggtaaagccc gttgccatca cctgaaactc
    29461 aacgccatgt ggctccagca gattgcgttc gccattaggc caggtgcctt taataaactg
    29521 cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt
    29581 ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat cctgggcctg
    29641 ggccacacgg gcatctactt tttctttcac tcgctgctga cgctgttggt agcgttcatc
    29701 actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat gccggtttta
    29761 cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc gggtgtttta
    29821 agtttctgtg gatcttcggt cgggaaggcg cttgcgcgca ttgcggtgcg cgtaccgcct
    29881 gggttaatgc agttgacgcg caggcgctgc tgatattcat cggccagtac ctgcatcatc
    29941 ccttcggtgg caaatttcga cgctgcatat gcaccccagt tggctcgtcc ctgacgtcca
    30001 acgcttgatg aagtaaagac cagtgaaccg gcgtccgatt tgagtaataa aggaagcagt
    30061 gcctgggtga gcataaaggt ggcattaacg ttgacctgca tgacgtcctg ccagacctgc
    30121 ggattttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg caaaacacca
    30181 tccagacgcg gataattaac gacaatgcgc tgtgccagtt gttggcaatt ttcggacgtg
    30241 caggtcagca aatcgagaat aaaccactgt ggctgacgcc cagtttcttc gtttatgtgg
    30301 ctggctacct gacgtaattt ttcttcatta cggcccaaca gaatcactgt cgcaccatag
    30361 cgtgcatacg tcatcgcggc ttcacgacca atcccatcgc tggctcccgt caccaggata
    30421 atgcgatcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc tcaacgacgt
    30481 tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga aacagaaggg
    30541 catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat actttcgctg
    30601 caataacatc tctgcgagac ggcttaacat gcctgttgta aactgtgagc caaagcgttg
    30661 tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa
    30721 tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat gttgctcaac
    30781 gtaataaacg ccagcgtggc gagttacggg tcaacaatct cagcgaacag tataaggaga
    30841 tgaaagaaga actggccgcg gcgctgatgg actcacatca gcaaaaacag tggcacaaag
    30901 cgcagaagaa aaagcacaag caagaagcga aagcagcaaa agcgaaagcc aaactgggcg
    30961 aggtggcaac tgacagtaaa cctcgcgtct gggtgctgga ttttaaaggc agcatggacg
    31021 cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca ttcaaaccgc
    31081 aggatcaggt tgtgctccgt ctggaaagcc caggtggcat ggtgcatggt tacgggttgg
    31141 cggcttcgca gctgcagcgt ctgcgtgata aaaacattcc tttaactgtt acggtagaca
    31201 aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac
    31261 cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt aaccgcttcc
    31321 tgaaaagcaa agatattgat atcgaattgc acaccgccgg gcagtataag cgcacgctga
    31381 ccttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag ctgaacgaaa
    31441 cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc gtctctggat attgaacagg
    31501 tggcaacggg tgaacactgg tacggacaac aggcggtaga gaaaggcctg gttgatgaaa
    31561 tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgtgaagtg gttaatgtac
    31621 gctatatgca gcgtaaacga ctcattgacc gattcaccgg cagcgcggca gagagcgccg
    31681 atcgattgtt gctacgctgg tggcagcggg gtcaaaagcc attgatgtaa aagacaaacg
    31741 cgaggctaag acctcgcgtt ttgctttaat caaccagatg atatttttct gaaagcacat
    31801 gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggctgttggc aatccttgtt
    31861 catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg acgccggtgg
    31921 cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc tctttcaaca
    31981 gcgtttcgcg gtcgatgggt tgtgtctctt tgttcattat ttactcctta aacaaggaca
    32041 ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca agagcgcctt
    32101 actggcaact ttggattttg catgctaata aagttgcgta tcggatttta tcaggtacag
    32161 tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt
    32221 gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc gtgaacagag
    32281 tcacgacaag gggttgatat ccgcagagag cgagtccata tcggtaactc gttgccagtg
    32341 gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag gtgaatatgg
    32401 gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac aagtatctgg
    32461 gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg accagtggct
    32521 cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag cctaaaaagg
    32581 atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc
    32641 actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa ctggctgaaa
    32701 aagccgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc attgcatggc
    32761 acctgcggga agtgattggt ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa
    32821 ttactaaaaa cgcgatccgc caggcattta acaaaccggg tgagctgaat attgatcgtg
    32881 ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg gtttcgccgc
    32941 tgctgtggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggtcc
    33001 gcctggtggt agagcgtgag cgtgaaatta aagcgttcgt accggaagag ttctgggaag
    33061 tcgatgccag cacgaccacg ccatctggtg aagcgttggc gttgcaggtg actcatcaga
    33121 atgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta agtctgctgg
    33181 aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt aaacctggcg
    33241 ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttggt tttggcgtga
    33301 aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact tacatgcgta
    33361 ccgactccac taacctgagt caggacgcgg taaatatggt tcgcgattat atcagcgata
    33421 attttggtaa gaaatatctg ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac
    33481 aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa tcgctgaagg
    33541 atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc gttgcctgcc
    33601 agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc gatttccgcc
    33661 tgaaagcacg cggtcgtatt ttgcgctttg atggctggac aaaagtgatg cctgcactgc
    33721 gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa aggcgatgct ctgacgctcg
    33781 ttgaactaac accagcccag cactttacca agccgccagc ccgtttcagt gaagcatcgc
    33841 tggttaaaga actggaaaaa cgtggtatcg gtcgtccgtc tacctatgcg tcgatcattt
    33901 cgaccattca ggatcgtggc tatgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa
    33961 tgggcgaaat cgtcaccgat cgccttgaag aaaatttccg cgagttaatg aactacgact
    34021 ttaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca gagtggaaag
    34081 ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct gaaaaagatc
    34141 cggaagaggg tggtatgcgc ccgaaccaga tggttctgac cagcattgac tgcccgactt
    34201 gtggtcgcaa aatggggatt cgcacagcga gcaccggggt attccttggc tgttctggct
    34261 atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag
    34321 tgctgaacgt gctggaaggc gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt
    34381 gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
    34441 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa ttccgcatta
    34501 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ttctgaaatg cacctgaaaa
    34561 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
    34621 ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc ggtgccatta cctgagctgc
    34681 cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg
    34741 ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc actggtggaa gagctttatc
    34801 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
    34861 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
    34921 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
    34981 aaggaaaaaa ataaccttta attctgtcag gtttttataa acaaagggtc gcgaaagcgg
    35041 ccctttttta ttgcatatta ttttttcttc acacctatac actaaggcta taaatgatat
    35101 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga
    35161 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
    35221 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact
    35281 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
    35341 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
    35401 aattcgtatc gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggtga
    35461 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta
    35521 cgcgttacca aacgttatca aaggctttat tgagcgttat ccccgcgttt ctttgcatat
    35581 gcaccagggc tcgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
    35641 tatcgccaca gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg
    35701 gaatcgggct attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat
    35761 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
    35821 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgttttca cggcaacgga
    35881 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
    35941 ggcggtggat ccggtcgccg atcccgacct tgtgcgcgtt gatgctcacg atatcttcag
    36001 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga
    36061 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt
    36121 gcgctccaat gaagaaattg aggtcatgtt taaagatata aaact
//
LOCUS       DADKXM010000023        34770 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.023, whole genome shotgun sequence.
ACCESSION   DADKXM010000023 DADKXM010000000
VERSION     DADKXM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.023"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(19..94)
                     /locus_tag="JQR05_001190"
     tRNA            complement(19..94)
                     /locus_tag="JQR05_001190"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="JQR05_001191"
     tRNA            complement(153..229)
                     /locus_tag="JQR05_001191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="JQR05_001192"
     CDS             complement(332..1717)
                     /locus_tag="JQR05_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5323402.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JQR05_001193"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JQR05_001193"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY5323403.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JQR05_001194"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JQR05_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY5323404.1"
                     /translation="MSLLEFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JQR05_001195"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JQR05_001195"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY5323405.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEQ
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JQR05_001196"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JQR05_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY5323406.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JQR05_001197"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JQR05_001197"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY5323407.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRT
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JQR05_001198"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JQR05_001198"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY5323408.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JQR05_001199"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JQR05_001199"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY5323409.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JQR05_001200"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JQR05_001200"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY5323410.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQGERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JQR05_001201"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JQR05_001201"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY5323411.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JQR05_001202"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JQR05_001202"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY5323412.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JQR05_001203"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JQR05_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY5323413.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JQR05_001204"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JQR05_001204"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY5323414.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JQR05_001205"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JQR05_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY5323415.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JQR05_001206"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JQR05_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY5323416.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JQR05_001207"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JQR05_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY5323417.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="JQR05_001208"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="JQR05_001208"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY5323418.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="JQR05_001209"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="JQR05_001209"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY5323419.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGNFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JQR05_001210"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JQR05_001210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY5323420.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22012..22293
                     /gene="ppiC"
                     /locus_tag="JQR05_001211"
     CDS             22012..22293
                     /gene="ppiC"
                     /locus_tag="JQR05_001211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY5323421.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JQR05_001212"
     CDS             complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JQR05_001212"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY5323422.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     CKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23958..24848
                     /gene="ilvY"
                     /locus_tag="JQR05_001213"
     CDS             23958..24848
                     /gene="ilvY"
                     /locus_tag="JQR05_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY5323423.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JQR05_001214"
     CDS             complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JQR05_001214"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY5323424.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JQR05_001215"
     CDS             complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JQR05_001215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY5323425.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JQR05_001216"
     CDS             complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JQR05_001216"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY5323426.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSLSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JQR05_001217"
     CDS             complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JQR05_001217"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY5323427.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29516..31162)
                     /locus_tag="JQR05_001218"
     CDS             complement(29516..31162)
                     /locus_tag="JQR05_001218"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY5323428.1"
                     /translation="MNGAQWVVHALREQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMVEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31165..31215)
                     /locus_tag="JQR05_001219"
     CDS             complement(31165..31215)
                     /locus_tag="JQR05_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323429.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JQR05_001220"
     CDS             complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JQR05_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY5323430.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31751..33271
                     /locus_tag="JQR05_001221"
                     /pseudo
     CDS             31751..33271
                     /locus_tag="JQR05_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JQR05_001222"
     CDS             complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JQR05_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY5323431.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33753..34592
                     /gene="hdfR"
                     /locus_tag="JQR05_001223"
     CDS             33753..34592
                     /gene="hdfR"
                     /locus_tag="JQR05_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY5323432.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34689..34764)
                     /locus_tag="JQR05_001224"
     tRNA            complement(34689..34764)
                     /locus_tag="JQR05_001224"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34729..34731),aa:Trp,seq:cca)
BASE COUNT         8477 a         9519 c         8777 g         7997 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttaccgt
      361 ggcgattaag cccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccga
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ccggtacgcc gtgacgggaa acttttgcca ttgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg ctgtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agagcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccaccgcgat caatgcgggt atccactgcg ccatctccgg gaaccaaaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag taagaccagg
     1441 cggtgagata gccaaaaaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaaa acgaacagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac gaagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcatgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gtacttccca ggtaaagtaa ttggctgagt
     3061 tcagcaccat actcgggcgc cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggtgaaaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta acgccagcca gaacatgcca acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggaaaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt ccagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccgcc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcactggca aatgctgttc cgtcatatcc tgccagaacg gattttccct
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc acgtaaagcg gcgacatgct cattgctgcg
     4501 gtcgccggag ttccccacca gaatggtcat ttttccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc tgttctcccc gcacctttgg
     4621 gtgcgttttg gcaaaaaagc tcaaatcgcc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgctg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt taatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgtgcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 cataaccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcccccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccatgg cgctaaggcg tctgacgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aagtgctggc ctcaatcagg ctgtcgtgag tgccggtatc cagccaggca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc gcgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttcaagcg agatagcgcg gaaattgtcg tcaaattcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagccaaa aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccattaagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcatatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccag catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtaccggaa ccgcccgcca ggataatacc tttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcac cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgaa ccgttttacg cattccactt
     9121 tcaaaggttt cctgcggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc
     9241 acgccgtgcg gcttgttcga agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcgc tcgccttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acaattcgct ggcgcgggcc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 atacacgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtcagatc cgggcgcatc tctgctaacc attctgccat cttctcggtt gaccccactg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtaggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcagt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actctcacgc ctatgaccgg tcaccagaat catcttttta tcggggtcga taaacgggta
    11881 atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctggtcac gcacccataa
    11941 cagtgcatca atgactgtat taccggtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 cgtcgtatcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagtacctga tcaagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggctcgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgtgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gagtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacgatcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagctctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcagttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa aatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccacgccg
    17281 acgcggaacc gctggcggaa gctaccggcc tgaagctggg cctggcttac ggtggtgatg
    17341 gctatgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcaccacgg
    17401 gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtagtgg
    17461 tactggacga agccgatcgc atgtacgatc taggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc
    18181 gcacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcttact gtagtaagtg attcgggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaatt
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgacctctc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgacttg ccgtcaatgc gggtaatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcgcgtctga tttatcaggg tgttgctcac accactggtg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcaag aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacact gcgtaatatt
    19321 caacgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa cgaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact tcaaacaagc tccgcaacac
    19501 gctgcttatc tggtgcgtaa cctggatctt cccggtttta cccccgcaca gaagaaactg
    19561 ctggcgacgc tactgctcaa ccagactaac ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggctatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaaaccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tctccggcct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cactgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagttcgc cgtactggcg acgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatactg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtggagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgtaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcagcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtgaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaactttt agctctgtgc
    21001 cgtaacccag ttcggagaac agacgctttt caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctgg ctcagcagca ccaggttttg cggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcatcg tcacccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ttcgtccacc agcaggtagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagaagt agcgtcggca
    21361 ataaaatcag atcatcgaag tcgagcacat tacaggcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcagtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcac ccgcggatca gatgggcgat tttattggtg ataacacgag
    21841 ttttaccgga acccgcaccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggaa aatcgctcaa tgaaagtcgg gaggggaaaa
    21961 taacagcgtg gtagtatagc cagcctcatc cttaccactc aaggcacgat catggcaaaa
    22021 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga tcttctcgtg
    22081 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    22141 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    22201 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    22261 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaaggcc
    22321 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    22381 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    22441 cagctgcgcg ttatctaccg caccttccgg aatagcttta cccaaatcgc ccggttgcag
    22501 ttctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    22561 ctcggcagta tcagagataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    22621 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gattcttcga tgatgccgga
    22681 atcaaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga tcatcagtac
    22741 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcgaa
    22801 ggcggttttg caggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    22861 cgccatcata ccggaggaga attcgccgga gatgatgtcg tccatatgtt tctggaacag
    22921 cggtgccatg atctctttca gctgttcaga caacgcgtaa gcacgcagtt tcgccgggtt
    22981 agagagacgg tccatcatca gtgtgatacc gccctgcttc agcgcttcgg tgatggtttc
    23041 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    23101 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    23161 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    23221 ggttgctgcc gcccatgctt tagcgatcgc catgccttcg cctttcggat cgttttccgg
    23281 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    23341 gccagggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    23401 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    23461 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    23521 ctgtgggatc agttcttcgt aagtacccac tttaaaacca ttttcggtcg ctttacgcca
    23581 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    23641 atcacgcatg ttcagaccct ggttcagacc ttgtgcgcca cagccgacga tgactacttt
    23701 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    23761 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    23821 tcctcgtgat gttgtgcttc ttattgttgc gtttgcgaga ttgaattcac tatatgacag
    23881 gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct gcaacgtcaa
    23941 cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    24001 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg ctctcacggc
    24061 agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat aaccgcacgg
    24121 tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt
    24181 atcagcagct gcgccacacc atcgaccagc aagggccgtc gctctatggc gaattacata
    24241 tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat cgcttccgcg
    24301 cggaacaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat gcaatggaaa
    24361 aagtcgtcac aggtgaagct gatctggcga ttgccgggaa gccagagact ctgcccggcg
    24421 cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc gcattgccct
    24481 gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta ccgtttatca
    24541 tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc aataaaatca
    24601 gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    24661 tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc gaaccggtgc
    24721 gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag ctgggcgtgt
    24781 gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag attttgccga
    24841 accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt
    24901 agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt tcgaacgccg
    24961 ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa atgttccagt
    25021 gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg aattcgaagc
    25081 tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag cgcacatgca
    25141 acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg ttgagcatct
    25201 gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca aagatgcagg
    25261 cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg ccaagcagtt
    25321 ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac aacgcttcac
    25381 gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag ttcacgttgg
    25441 caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg atatattttt
    25501 tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca tcttcgaata
    25561 aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg tcgagatact
    25621 cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct tcggcgaaca
    25681 gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc aggcaggcgg
    25741 agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg atcagcaccg
    25801 ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg agatgggcgt
    25861 cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc ggatggtcga
    25921 acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta gctttcgctt
    25981 cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc gcatcgactt
    26041 tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac cgcgcagaag
    26101 aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg tgcgcttttt
    26161 gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag ctgtgcactg
    26221 gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac agtttttcca
    26281 ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc actgctctta
    26341 aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt attaaccccc
    26401 cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc tggcgtaggc
    26461 gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt tgtcaccccg
    26521 agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatgcc
    26581 acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa tgctgccgcc
    26641 gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc cagagaaacg
    26701 accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga agctggttgg
    26761 gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga tcaccaccac
    26821 atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct ggctttcgta
    26881 cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg ttttcacgat
    26941 gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt tgctgtaggc
    27001 gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat cccaacggca
    27061 atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga acatattttt
    27121 taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg gcaacgtcag
    27181 gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt caccgagaat
    27241 accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg tgctcggcgc
    27301 aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca tggtgaagtc
    27361 gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag agccgcccat
    27421 cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat tgcgcggcag
    27481 tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc gtttaccggc
    27541 attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt tgcccggctg
    27601 cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca tcccggagca
    27661 ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt cagaaacttt
    27721 cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct gatcggacag
    27781 tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg
    27841 cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc agaccatggc
    27901 gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt cgcgagatgg
    27961 aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg caatggtgtt
    28021 gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt taccgagatc
    28081 gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg
    28141 cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat
    28201 attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt
    28261 gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt
    28321 gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag aaggcttgct
    28381 gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg tctacgctgc
    28441 gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc aggtacaggg
    28501 attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc agtttgatga
    28561 tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg aacagcacac
    28621 catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg ttcacatcca
    28681 gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc agggaagaga
    28741 gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg cggttccagg
    28801 aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gctccccacg
    28861 ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact cccatgccaa
    28921 catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt ttgcggatca
    28981 cgtcacgaca agcttccatc agctcatcaa tgctcagtga aaccgggaag cgatagattt
    29041 tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt
    29101 gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc gcgtgcgaca
    29161 tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag
    29221 ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgtg
    29281 gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac
    29341 agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg
    29401 ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa
    29461 acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg
    29521 ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc cagacgttct
    29581 caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga
    29641 gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg aaggcgctgg
    29701 ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt tcctgaaaaa
    29761 acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca
    29821 acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg aaagagccgt
    29881 caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca actgccgccg
    29941 gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc gggcgagtgt
    30001 gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc acgacgcaat
    30061 ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata gcgtcaccgg
    30121 gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc tgccagtcat
    30181 tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct tgtaatgcca
    30241 catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg ataacactgg
    30301 cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcacccacgg
    30361 cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg tgcatcccca
    30421 gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc agcgtacagg
    30481 tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc tgcgccatcc
    30541 ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc tggcgcgctt
    30601 gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac cacggttcca
    30661 gatcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga ccaggacgac
    30721 ctgagctggc aacgtcgaat gcttcaacca tgatgcgcgg caactcttcc agagactgca
    30781 ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact tcctgaaatg
    30841 cgtcggtgcc gataaacggt gcggacacct gaccagtgat ggcaacgaca gggatggaat
    30901 ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca gacgtggcga
    30961 tacatacgcc agttttaccg gtagcacggg cataaccgat agccgccatt gccgcaccct
    31021 gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg taaaccggca
    31081 taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgttcccgc aacgcatgta
    31141 ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt
    31201 tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa aacccccgga
    31261 cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt cctcgtccaa
    31321 gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc aggctaatca
    31381 ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga
    31441 gttaccatag attttgcgaa tagcacaaga tattttttgg cctattttta tgatggcttt
    31501 aataatttca atagaaattg ttgtttttta aaaagaaaat agtgagtgaa aaaattcaat
    31561 ttcagtataa atgttgtccc cttcctcgta gttcatcctt tgtccgctgc atttttgcga
    31621 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
    31681 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca
    31741 aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag taaatgcgcc
    31801 cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga tggtgggctt
    31861 accagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
    31921 atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc tgccaaaaga
    31981 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
    32041 agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag gcgctctgcg
    32101 tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
    32161 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc
    32221 cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa
    32281 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
    32341 gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt tactgattgg
    32401 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt tgccagattt
    32461 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
    32521 acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaactgc
    32581 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
    32641 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
    32701 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
    32761 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
    32821 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
    32881 ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    32941 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    33001 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    33061 caaacttgag agcaaagatg cgatgtggtt ggaagaaacg ctgatccatc tggggttatc
    33121 gattcgtgcc tagcagcggt tactgaaagt tgcacgaacc attgctgata ttgatcagtc
    33181 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    33241 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    33301 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    33361 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    33421 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    33481 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    33541 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    33601 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    33661 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    33721 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    33781 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    33841 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    33901 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    33961 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    34021 agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggttgggac
    34081 gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    34141 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    34201 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    34261 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    34321 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca
    34381 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    34441 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    34501 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt
    34561 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    34621 aaggatttac ttcggagagg attatttcag ataaaaaaaa atccttagct ttcgctaagg
    34681 atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg gagaccggtg
    34741 ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DADKXM010000024        34753 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.024, whole genome shotgun sequence.
ACCESSION   DADKXM010000024 DADKXM010000000
VERSION     DADKXM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.024"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(71..1324)
                     /locus_tag="JQR05_001225"
     CDS             complement(71..1324)
                     /locus_tag="JQR05_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY5323433.1"
                     /translation="MSGSNTAISRRHLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1556..2887
                     /gene="argA"
                     /locus_tag="JQR05_001226"
     CDS             1556..2887
                     /gene="argA"
                     /locus_tag="JQR05_001226"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY5323434.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAENMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2949..4775)
                     /gene="recD"
                     /locus_tag="JQR05_001227"
     CDS             complement(2949..4775)
                     /gene="recD"
                     /locus_tag="JQR05_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY5323435.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMFEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4775..8317)
                     /gene="recB"
                     /locus_tag="JQR05_001228"
     CDS             complement(4775..8317)
                     /gene="recB"
                     /locus_tag="JQR05_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY5323436.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEE"
     gene            complement(8310..11198)
                     /gene="ptrA"
                     /locus_tag="JQR05_001229"
     CDS             complement(8310..11198)
                     /gene="ptrA"
                     /locus_tag="JQR05_001229"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY5323437.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKDGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AIITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11374..14742)
                     /gene="recC"
                     /locus_tag="JQR05_001230"
     CDS             complement(11374..14742)
                     /gene="recC"
                     /locus_tag="JQR05_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY5323438.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKTLVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14755..15078)
                     /locus_tag="JQR05_001231"
     CDS             complement(14755..15078)
                     /locus_tag="JQR05_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY5323439.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15063..15470)
                     /locus_tag="JQR05_001232"
     CDS             complement(15063..15470)
                     /locus_tag="JQR05_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY5323440.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHCWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15467..16030)
                     /locus_tag="JQR05_001233"
     CDS             complement(15467..16030)
                     /locus_tag="JQR05_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY5323441.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16021..16491)
                     /locus_tag="JQR05_001234"
     CDS             complement(16021..16491)
                     /locus_tag="JQR05_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY5323442.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLYQQGETEGCL"
     gene            complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="JQR05_001235"
     CDS             complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="JQR05_001235"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY5323443.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="JQR05_001236"
     CDS             complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="JQR05_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY5323444.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="JQR05_001237"
     CDS             complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="JQR05_001237"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY5323445.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="JQR05_001238"
     CDS             complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="JQR05_001238"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY5323446.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21976..22665
                     /gene="mutH"
                     /locus_tag="JQR05_001239"
     CDS             21976..22665
                     /gene="mutH"
                     /locus_tag="JQR05_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY5323447.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22734..23447
                     /locus_tag="JQR05_001240"
     CDS             22734..23447
                     /locus_tag="JQR05_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY5323448.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23585..23803
                     /gene="ygdR"
                     /locus_tag="JQR05_001241"
     CDS             23585..23803
                     /gene="ygdR"
                     /locus_tag="JQR05_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY5323449.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23911..24951
                     /locus_tag="JQR05_001242"
     CDS             23911..24951
                     /locus_tag="JQR05_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY5323450.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24984..26177)
                     /gene="lplT"
                     /locus_tag="JQR05_001243"
     CDS             complement(24984..26177)
                     /gene="lplT"
                     /locus_tag="JQR05_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY5323451.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26170..28329)
                     /gene="aas"
                     /locus_tag="JQR05_001244"
     CDS             complement(26170..28329)
                     /gene="aas"
                     /locus_tag="JQR05_001244"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY5323452.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSCQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28915..29946
                     /gene="galR"
                     /locus_tag="JQR05_001245"
     CDS             28915..29946
                     /gene="galR"
                     /locus_tag="JQR05_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY5323453.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JQR05_001246"
     CDS             complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JQR05_001246"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY5323454.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31337..32272
                     /gene="lysR"
                     /locus_tag="JQR05_001247"
     CDS             31337..32272
                     /gene="lysR"
                     /locus_tag="JQR05_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY5323455.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JQR05_001248"
     CDS             complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JQR05_001248"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY5323456.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33080..34498)
                     /locus_tag="JQR05_001249"
     CDS             complement(33080..34498)
                     /locus_tag="JQR05_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY5323457.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8508 a         9517 c         8592 g         8136 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaag cgccattcag
       61 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
      121 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
      181 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
      241 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt tttccgagct
      301 tattcagtac cgctttacca aacttaaggc tgtcagcaat agtcagcgac tgcaccatat
      361 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
      421 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcgcct ttggttgaga
      481 gcgcaaacac cgaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
      541 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
      601 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
      661 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
      721 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
      781 ctttacccgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
      841 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
      901 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
      961 ttacgttttg ctttaattca aaaaccatgc gtacggtttg cgggtcaaat tgcccgacgc
     1021 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     1081 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
     1141 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
     1201 agacgcgcac cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata
     1261 gccacatagc gcccgcgcct tgcagtaaat gacgacggct gattgcagtg ttggatcctg
     1321 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     1381 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     1441 tattcacggc attactgata aaaaagtcgc tctcgcataa aattcacact tgcaccctgc
     1501 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaaggggt gtgccgtggt
     1561 aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac
     1621 ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
     1681 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
     1741 ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg aaccgctgta
     1801 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
     1861 aacattgcaa ctggatatta ctgctcgcct gtcgatgagt ctcaataaca cgccgctgca
     1921 gggcgcgcat atcaacgtcg ttagtggcaa ttttattatt gcccagccgc tgggcgtcga
     1981 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatcca
     2041 tcgtcaactg gaaagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
     2101 cgagagcttt aatctgacct cggaagagat tgccactcaa ctagccatca aactgaaagc
     2161 tgaaaatatg attggttttt gctcttccca gggcgttact aatgacgacg gtgatattgt
     2221 ctccgaactt ttccctaacg aagcgcaagc acgggtagaa gcccaggaag agaaaggcga
     2281 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
     2341 gcgccgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc
     2401 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
     2461 aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
     2521 ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg
     2581 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
     2641 aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct
     2701 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac
     2761 gcgcagtatt cactggttcc aggaacgtgg atttaccccg gtggatattg atttactgcc
     2821 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
     2881 agggtaagcg atgaaaatca ccggatgcgg caggcgtaac actcgtgcgt cgcatccggc
     2941 aattacgttt attcccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga
     3001 gtggcgattg ccgcacttaa tatacgctca tcggcataca gcgacagacg acgacgcgcg
     3061 cgggtcaccg cggtgtatac cagctctcgc gttactaccg gcgtgcgttg gctcggtaaa
     3121 atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta
     3181 gtttcgtgct ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca
     3241 aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac
     3301 ccaagcgcgc tgtcattacg ggcaatcatc accggtcggc cttcgtacca acgagagtgc
     3361 ggatgacgat gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca
     3421 gccacgccaa acggcccttc ccgcagggcg cacaaaagct ggtactcatt gaacgcctga
     3481 atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc gtaacccgca
     3541 agagcttcct caaacatcgc aatataatct tcgccgctct gtaaaagtcg tttttcgata
     3601 tcagtaaaat cctgctgaaa aacagttttc actgccgttt tatcaccacg gttgatcgcc
     3661 gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag
     3721 agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agtccccgtc
     3781 aggcggctta gctgcctggc acgctcggcg gtaaagcccg cgttggcata agcgcagata
     3841 tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc
     3901 actcgcgcat gatctggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt
     3961 gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa
     4021 cgctggctac ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg
     4081 cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt
     4141 aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg
     4201 gccatttgaa ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg
     4261 ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg ccgctgcaac tttttgccag
     4321 ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca
     4381 acctcaatgg catgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac
     4441 atgcgattca aataaagacg atcgccacag aggatcatcg gcgtgggttc atctcccctg
     4501 ctgaccgctt gagaagccag caagcattct tcccaatttt gtagctcacc gatttcactg
     4561 acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga aagcggcaaa
     4621 cagacgtgtc cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga
     4681 tgttcatctc ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc
     4741 tccacagctt ccagtaattg cttttgcaat ttcattactc ctcctccagg gtcataccgg
     4801 caaacatctc atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt
     4861 gttgcggatg ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgaa
     4921 gatcatagtc gtaatcagca atgcgatggc gcagataacg atgcagcgcc agggtataaa
     4981 gctgatattg cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt
     5041 aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt
     5101 cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca
     5161 gcggcgggca gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg
     5221 cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg
     5281 aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc
     5341 actcggtcaa caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta
     5401 cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg
     5461 tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc
     5521 tgaccacgcc tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac
     5581 cacgctgctg caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta
     5641 gtgtcctcgc attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac
     5701 cagtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa
     5761 gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga
     5821 cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc
     5881 aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt
     5941 cgacctccgc gaggtcgacg ctttctggcg cagcattaag atccaaaact gcctcaaacg
     6001 agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc
     6061 agaccagcgg atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt
     6121 tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt
     6181 gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgaagca
     6241 gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt
     6301 tttctgcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc
     6361 gccagatttg ccgataacca tcgaactctt cgactaccgc atcccacgca tgttcgtcat
     6421 tattcagcgt ttcaatatcc agcgcattca gccccatcat tgacgttgcc agcgcactgc
     6481 gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa caaccaaagc atttcctgcg
     6541 cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg atttccagca
     6601 acgttaaggc atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acgctgatgt
     6661 ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc
     6721 cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgatagt
     6781 cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac
     6841 ctttaaatac aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta
     6901 tttcgcgaaa catgaacgcg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca
     6961 ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg ggcgtgaact tcactacgcg
     7021 ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc
     7081 caatcagcaa caatgcggtt tccggctgat ggtgccagat acggcgaaaa attcggtact
     7141 gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg
     7201 ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt
     7261 catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag
     7321 ccaatgcgcg ggtgatcacc agatcgcgga tcgacaatgg ttctgcaagc agttgatcga
     7381 tcgcctcaaa cagtggatgt cgcggggttt cccccccggc cttcgtgcga tcttctaaga
     7441 aacgctggga gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg
     7501 cccaggcgct gatcttttcg atccatttag cctgattgct acggttaaac ttgcgtcgat
     7561 caataccaga agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt
     7621 ttaccgtatc aatacgcgcc acaatttgcg cgtggcggga agccagcgtt tcatcatcgg
     7681 gcggcggtgc tttgataacc ggcgcttcgc cttgcagata acgattaata tcgcgcagca
     7741 acgcctgcgg ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt
     7801 agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa
     7861 tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc
     7921 aaaagccgtg aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc
     7981 actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gcgttcgtac agtggattgt
     8041 cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccgc
     8101 gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acgatcagcg
     8161 ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag gcgcaaatag agcgccgcaa
     8221 tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca
     8281 agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt
     8341 gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg
     8401 gctttcccgt tctggctgcc ggaaatctgc gacagaatag ccatgccttg cggctcgacc
     8461 accgcctgat ggaagaaatc agcaagtttt tgcggcgtca gcagttttat ctgggccacg
     8521 attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat
     8581 gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattatcgc ctgctggatt
     8641 tgcgcaaact catctggctt catcgctcgc aattttgcct ctgcggttgg gaaaaacgcc
     8701 ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg
     8761 ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa ttgttcttcg
     8821 gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct
     8881 gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg
     8941 ctgttaccgt ctttttcaaa gatgacggat tgttttttat cgaccactac atctttgttt
     9001 cgacaccact ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt
     9061 gcctgggcct cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag
     9121 gcgtcgcgat aggccagcac ctctttcaac gtaatggagg gcaaaatttt acgccgttca
     9181 tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac
     9241 gctttaccct tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc
     9301 tgctcttccg tagcggtata gctaaagtac ccctcgagca atgcctggaa cagctgcggc
     9361 agacgctggg tgtaaccatt agcattaacc ataaggccgt tgttagcgtt ggtggaaaaa
     9421 cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa
     9481 tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa
     9541 atcaggctga catcagcttt gggctcgctg gcaaaataac ggcttggcgc atacaccacg
     9601 cgcagattcg actcatcaac aatcagctct ggatggtcgt atttcttctc tgacttaatc
     9661 agcgagaaat catcaggaat ataagggtta agctctggca aagagagcgc aatgtcggcg
     9721 gcttttttct gccagtcggc gaaagtttgt gcgctgattt tatcgacctg atacggcgca
     9781 tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc tgatatacca gatacgcgca
     9841 ttctgcggcg tcatcatcgc cagacgttcc tttactgctt tagcatcgta ccgatcggca
     9901 atattgactg catccagcgt atgctcaaca ggaacgcgaa tcatggtatc tgccagccat
     9961 tcgacgtaat ccatatcacg ggtgatcgac ggataacgga agtcgatatc cagcacattc
    10021 gccagttcat cgaagtattg tttatcaatg cctttttcac gtaacagatt gagatagcta
    10081 aaaattgccg ccacaacctg atcgcgatta gccaggcctt tatcggttaa agacgcagag
    10141 atcgctaata cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca
    10201 actaatccct gcttttgcag ccagtcagaa agtgtacctg ggctgcgatt gccaatcaga
    10261 taggtaatca attcatcggt tttactacgg aacttcgctg agttgttatc gatgcgaaac
    10321 tcaacgcgca acactttacg cggcagcgca gggacgtaat gaatgataat gcccttttgc
    10381 gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga
    10441 ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg gtttattact gtaaataacc
    10501 gccttcatca aattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc
    10561 ggattaccag gtttgtcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg
    10621 tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc
    10681 gccatggtta attcagcgtt cactgcatta cgctcacgtt cggcgtattt cttgtcgagc
    10741 aagggttctg caatagcatc ggccaggcgg tctaccgcac cgggcaaggc gtcgttctca
    10801 acttccagat agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc
    10861 attttgagat attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc
    10921 atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg
    10981 ggcactacca gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc
    11041 ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg
    11101 gtttcctgaa tcggctgcca tcccgtttct gcctgactta atggtgccca gagggcaaca
    11161 aacaacaata atgctttgaa ccaggtgctg cggggcattc acggacctca taatcaactt
    11221 tattttctgg acagattcaa caggttaacg caaatcatct gccagaattt aatcttgtgc
    11281 tgcacgagtc agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt
    11341 aagcggatag attgcgcaat ctttatacaa cactcatgac tgattaaagc gaaacagcgg
    11401 taacaggaaa cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg
    11461 ccagagccgt tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta
    11521 agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc
    11581 gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa
    11641 tggcgcggac attccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg
    11701 ttcggctgca agcggcggaa aacgccactc accgtctttg cgtagaaaaa ggcgactttc
    11761 gccattacca ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc
    11821 cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca
    11881 gccagttatc tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg
    11941 acaggcaatg actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat
    12001 ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc
    12061 ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag
    12121 tccttccaga ataaatggct cggtgtctgg gatttcgctg tcttcagtac ggaagttcac
    12181 ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc
    12241 cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt
    12301 accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg
    12361 gtagttttgc ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg cttttaccct
    12421 tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat
    12481 gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga
    12541 acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag
    12601 gtagcggtcg tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc
    12661 aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac
    12721 cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag
    12781 aaaacgctgg ctgatacgtt cttgatccag acgctgtgcc agttcatcac gcaatagtga
    12841 cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg
    12901 ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag
    12961 catatcgcga caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg
    13021 ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc
    13081 gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta
    13141 gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc
    13201 cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg
    13261 gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc
    13321 cagcagcgcc agcacatcct ctgacacaaa gcgactgtca ggcagtgata acaggctgat
    13381 aaacgcttcc agtaccggat gtgactgccg cgcacgacgg tcggaaatgg cgtaaggcag
    13441 gtaacgatcc gcaggtgcgc taccaaacac tgcctgaata aacggactgt agctgtcgat
    13501 atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc ggggcttcct ccagcatcgc
    13561 cagcaagcga tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaatgt
    13621 gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat
    13681 gttcacaccc gcaacggcgc ggttttccag ttccagaatg tcagactgaa tgttatgcag
    13741 caggttatct ggcgtcacat cgacaaaagc gtccagctcc tggctgctct ccaggtcaga
    13801 aaggagataa atgtagtcgc gcccaagctt cccccatgag gccagcagcg ggttgccgac
    13861 atcctgttca ccatcgctgt taaagagctg tccggcattt tcgctatcgc gaaatagcgg
    13921 caattcgcga tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg ccagataagc
    13981 aggatcttta atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc
    14041 aatatgttta cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc
    14101 gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc
    14161 gataaagcgc tgatagagat tcgcgcggtg ccagcgcggt tgtccgagtt catgggtata
    14221 ttccaccagc gtcttccaca acggggcttg ccaggcctgt gcttctccca gcccttcaac
    14281 caaatgtcct gtttcccact gtgccagcca gtccggacga tagaccagat actggtcaaa
    14341 caggtccgcc gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag
    14401 ataatgccgc aacagggtaa agtcttcgcg ctccagcaat tgcggcagca gagtcatcag
    14461 tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg
    14521 gacgaacata tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatacc
    14581 aaacttttgc gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat
    14641 catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc
    14701 ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg
    14761 acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt
    14821 aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc
    14881 aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta
    14941 ctggtttctg ctggcgagac tgttcattaa tgttcgctgg atacccgata acgcagtgac
    15001 aatcatcacc atcagcacca tcgcaaccat tacctccggc aggctaaagc cttgttgatt
    15061 cttcagggaa gctgacataa tgccccctct ttcagcggac aaaaatcgct ccagccgcgt
    15121 ggcgaaaaaa caatttttcc atcaatgact tcgcctgttc gccacaacga aacgccttca
    15181 taaccggcaa tcaataaggc ttcattatct gccagtaaac gcaaacaaac ctgggcatcg
    15241 gttccagcgt actgcgagca ctgaactgct ggctgcgtct gccagcagtg catttttccc
    15301 cacgccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc catgctcacg
    15361 cgagaggcaa aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc
    15421 agcagcatca ggaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc
    15481 ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca
    15541 tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt
    15601 cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg
    15661 caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcggactctt
    15721 ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt
    15781 caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc
    15841 tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agtgccagct
    15901 tacgagtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgca gcccccagca
    15961 acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt
    16021 ttacaggcat ccttctgttt ctccttgctg ataaagccgg agtcttcccc acggcgaaac
    16081 caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt
    16141 gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg
    16201 tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac
    16261 aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt
    16321 ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccaaagccg
    16381 ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca tgaccaaaat
    16441 cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt
    16501 atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga
    16561 ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaacg
    16621 gagggtaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc gtaattagat
    16681 agccaccggc gctttaatgc ccggatgtgg atcgtagcct tcaatctcaa agtcttcgaa
    16741 acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca gcggacgcgg
    16801 ttcgcggctt aattgcagat gagtttgatc catatggttg ctgtacaggt gcgtatcgcc
    16861 accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca tatgcaccag
    16921 taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg agcgctgata
    16981 aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat ggcacggtgc
    17041 cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc ggcgcgaatc
    17101 cgggtcgttt ttcagctggt tcagtaccgt agtaatctgg tcaatatgac gaccatctgg
    17161 cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccat tttcatcggc
    17221 ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag tatcgccctg
    17281 caggaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttgg ttgtcaccag
    17341 cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa gcgttccggt
    17401 tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca gttctaaata
    17461 ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg cccagaccat
    17521 catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc tgatgtactg
    17581 cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa aactcaacaa taatgcgaaa
    17641 cgcgccgtag ccaatcagga acaagcctga gacagctccc attgggcgcg gtttacgaat
    17701 atacaggttg aggataataa acagcaccac accttccagc agcagttcgt aaagctgtga
    17761 tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg ggttggtttg
    17821 cagcagcaaa atatcttctg tacgggagcc tgggaacagc atggcaaacg ggaagttcgg
    17881 gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg caccaagacc
    17941 aaacggaatg agtggtgcga taaaatcaga gacctggaag aaggaacgtt tagtacggcg
    18001 ggcgaagata atcatcacca cgataacgcc aatcaggccg ccgtggaaag acatgccgcc
    18061 gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaaat tgtagaacag
    18121 aacataacca atacgtcccc cgaggaagac gccgaggaag cccgcataga gtaagttttc
    18181 aacttcattt ttggtccagc cactgcccgg acgattcgcc cgacgtgttg ccagccacat
    18241 tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca cgggtcctat
    18301 tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca tctgtcacca
    18361 caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg caggtttcag
    18421 gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg ataccgttaa
    18481 aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca tgccgcgacg
    18541 ctccataaag gctgcaacct gatgacgaac ttcggtcgcc agttgcgctt ccagactacg
    18601 ctgcgcaaga ttttctgctt cggcaaaatc aatgcgccgc agcaggtatt ttacccgcgc
    18661 tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga ttgccacgca
    18721 catgggatcg cccgccattt caccgcacaa acggagatcg ataccatgta tttccgcttc
    18781 ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcgt aaatgttcgc
    18841 cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt tggtgccaac
    18901 agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg acggcacttc
    18961 cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct cgacttcacg
    19021 tccggcacgt tcaatcaggc ggcgtgcttc gtcaacttca tcgaggctgg tgaccatcgg
    19081 caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc gcacctggat
    19141 caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca ggcatggatt
    19201 ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca gcgtacgcaa
    19261 ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca cctgttcttc
    19321 ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa gaccgatgcc
    19381 atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccagcat tgagcatgac
    19441 ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt ccgccagacg
    19501 gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta ccggctccgg
    19561 atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat gcagcaccga
    19621 aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca tgatcgcagc
    19681 atgggagttg gctgcgccat ctcgcacgac aacaccgact aagcgatcct ggggcagctc
    19741 agcaagagtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg gccaggcgtt
    19801 cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg cacgtaaatc
    19861 gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg caaatttttc
    19921 aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg caaacaattc
    19981 gcgacgcagc cgggtgtccg aaagcaggtg cgagtaaaga tcgaaaatag ccgccgtttc
    20041 tttttgtgct ccggcggcaa agcgtttgct gtagcggcga aactcgtttg ccgcttcttc
    20101 cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg cctgatacac
    20161 ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca caccaggtgc
    20221 tgccggtaat gcgcggattc gcgtctggcg atattgccca aacagggcag tcaactgcga
    20281 ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt cttcactttc
    20341 gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact ggcgacgttg
    20401 aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg ggatgtattt
    20461 gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc tgccaaccag
    20521 accgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt ttttcagccc
    20581 ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga ccgaacagac
    20641 ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct cattcaggcg
    20701 tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa ttggcgtgac
    20761 ttaacctctt ttacgtcgat aagcagatgc gttttgtggt tttggcgtat tttcctgcag
    20821 tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac gtttaaatga
    20881 caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa actctggtgt
    20941 actgctggtt tgcatattga tttctgcatc gccgcttacc agctgcaaga gaaaccattt
    21001 ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt tcggtaattt
    21061 gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc ttaatcctac
    21121 ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt tgattccgcc
    21181 ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct gcccctggcg
    21241 attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca ccggactacc
    21301 tcaaaataaa gctttatata cgaatgattg tttcatactc caggaagacg gtaaaccact
    21361 ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt gcagagttat
    21421 aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata gagttgtgaa
    21481 gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga tatagacatt
    21541 ttaaaatatt cattattagt caatgcatta catgtaattt gagtaatgaa aaagagtaat
    21601 tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca atgccgtatt
    21661 tatccacaga atgagccact aagttaagca ctgaaccact aaaaactgga gtttcgtcgc
    21721 acgtcaaggc tgtaaatgaa aacagtagtg gaggtttttc acagttatcc cagctttctg
    21781 tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa aacctattgc
    21841 agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcaga gaattgaaca
    21901 acacatgtgg aaaaagttac actgcgaata ttcggcacat aattgctgtt tgttttttaa
    21961 ccaaggtatc atgacatgtc ccaacctcgc ccactgctct ctcctcccga aactgaagaa
    22021 cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact ggcggcactt
    22081 gccgggctgg ttacgccaga gaatttaaaa cgcgataagg gctggattgg cgtgttactg
    22141 gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc tgctctgggc
    22201 gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac aacattcgtt
    22261 tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca tgtgcgccac
    22321 aagctcaaac gcgtactgtg gataccggtt gaaggcgagc gcagcatccc gctggcgcag
    22381 cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg acagctacgc
    22441 gaagactggg aagaattaat ggatatgatt gttctcggtc aggttgagcg gatcaccgct
    22501 cgtcacgggg aatatttaca gataagaccg aaagcagcga atgcgaaagc gcttaccgaa
    22561 gccattggtg cccggggcga acggattctg acgctgccac gcggctttta tttgaagaag
    22621 aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc gctttgacct
    22681 gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt tagatgttat
    22741 ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacgctgacg ctgctggaga
    22801 tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag cttcccacag
    22861 cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg cgtctggcgc
    22921 tgctggcatc aatctcttgg gttacgcgcc tgacaaatcc gctttttaca atattcagtc
    22981 aggaaatttc tgcccgtgat ttgattctgc ttctgggtgg cttgttcctt atctggaaag
    23041 ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa acacgcgttt
    23101 catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttc agcctcgact
    23161 cggtgattac cgctgtgggt ctgtcagatc acctgtttat tatgatggca gccgtggtga
    23221 ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc gaacgccacc
    23281 cttcggtaaa aatgctggcg ctctctttcc tgattctggt gggctttacc ctgattctgg
    23341 aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc ttctctattg
    23401 cggttgaaag cctcaacttg attcgcaaca aaaagaatcc gctctgatac tccgtacgct
    23461 ctcctgcagg agggcgtact cactcctttt accctcacag tttaagattt tcctgctttc
    23521 aagatatata acgtcggttt ataaacagac tattatcata ggtaggccaa atgaggataa
    23581 ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg
    23641 gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa
    23701 acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat
    23761 gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc
    23821 cggctggcct taagattttt ctctttccct tttcccttcc ctctgccatt tttatattcc
    23881 ttatgtcgtg attataaaaa ggaaacggct atgcaatatc accgtatacc ccacagttcg
    23941 ctggaagtca gcacgctggg gcttggcacg atgacgtttg gtgaacagaa cagcgaagcc
    24001 gacgcccacg cacaactcga ctatgccgtc gctcagggca ttaaccttat cgacgttgcc
    24061 gaaatgtacc cagtacctcc gcgccccgaa acgcaagggt taaccgaaac ctacgtcggc
    24121 aactggctgg cgaaacatgg cagccgcgaa aagttaatta tcgcctccaa agtgagcgga
    24181 ccgtcgcgca ataatgacaa gggcatccgc ccggatcagg cgctggatcg gaagaatatc
    24241 cgcgaagcgc tgcatgacag cctcaagcgc ctacagactg attacctcga tctttatcag
    24301 gtgcactggc cgcagcgccc gaccaactgc ttcggcaaac tcggttatag ctggacggat
    24361 tctgcgcctg cggtttcgct gctggatacg ctggacgcac tggcagagta ccaacgcgcg
    24421 ggaaaaattc gttatatcgg cgtgtcgaac gaaactgcat ttggcgtaat gcgctacctg
    24481 catctggcgg acaaacacga tctgccgcgt attgtcacca ttcagaaccc ttacagtctg
    24541 ttaaaccgca gttttgaagt aggtctggca gaagtcagcc agtatgaagg ggtcgaactg
    24601 ctggcctatt cgtgcctggg tttcggcacg ctgaccggga aatatctcaa tggtgcaaaa
    24661 cccgctggcg cacgtaatac gctctttagt cggttcaccc gctatagcgg tgagcaaacg
    24721 caaaaagccg tcgcggcgta tgttgatatc gccagacgtc atggccttga tcctgctcag
    24781 atggcgctcg cgtttgtacg ccgtcaaccg tttgttgcca gcactctgct gggcgcaacc
    24841 acgatggatc agctgaaaac taacatcgaa agtttgcatc tggagttaag cgaagacgta
    24901 ttagctgaaa ttgaagcggt acatcaggtt tatacttatc ctgcaccata aagattaacg
    24961 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
    25021 gccagcgcaa atagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
    25081 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
    25141 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
    25201 agcggaacga caaaaaagcc ccccagcacg ccaatcagca tcaacaaggc ataggctggc
    25261 agcagctcgt gttgcaggga gaaaatcagt accaccacgc caatcaaaat cccggctggc
    25321 atacagcgtg acacggtttc cagcgtaacc agcttcgctg ccgcacctgc gccaaccacg
    25381 ataccaatcg ctaccatcgc gttgagatag gtcggcgtag cgttatcggt aatgcccagc
    25441 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
    25501 ctggtgccca ccagcgaaaa acgcgtttca ccattgcacc atagcgaggt gcaggcattc
    25561 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
    25621 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
    25681 gcgaccagga catgccagtc agccagcaca ccaccggcta cggaaccgag taaaatcgcc
    25741 gctatggtag aagcttccat taaaccgtta gcttttacta acttactacc cgtggttaat
    25801 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
    25861 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
    25921 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
    25981 gccaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
    26041 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gcgcgttatc gccaaacgca
    26101 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
    26161 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
    26221 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
    26281 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
    26341 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
    26401 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
    26461 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
    26521 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
    26581 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
    26641 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
    26701 gcgtgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
    26761 gttgatagaa acgacaggcg cgcattcggt cacaccgtag ccttcaagga tgcgcaggcc
    26821 aaatttatcc tgccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac
    26881 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
    26941 cgaggtaccg aacaacacgg tgcaactgcg gtcatacacc aagtccggca caatgcggta
    27001 atgcagcggg cttggataaa ggaacacttc tgcacctgta agcagtggcg taaacaggcc
    27061 taccgtcagc ccaaaggagt gaaacagcgg taatgccgac ataaagcgat cgttggtggt
    27121 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
    27181 gacgcctttt ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
    27241 ctgctgtttt acctgcgcca ggcgcggcat cagcaaatga gcgaagatcc atactttgtc
    27301 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
    27361 ctccggcaga tgccagagtt tgcctttatc gagaaactgg caggaagtga agatggtttt
    27421 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
    27481 tgcgggaatg cggcgacgcg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
    27541 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
    27601 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
    27661 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagagtttc
    27721 acgcgggcgc accgccatac gcgcttccat cattatttga tgcagcattt cgccagcgat
    27781 tttgcgacgg tcacgggcac gcggcgcatc cggcatcgcc acctgcgttg gaggcaaaat
    27841 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
    27901 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
    27961 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgta gtggtgatgc gtccttcagg
    28021 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tagccatagg
    28081 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
    28141 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
    28201 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
    28261 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
    28321 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
    28381 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
    28441 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
    28501 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
    28561 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
    28621 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    28681 ttcacctatc aatacctctg gaatcaccac tttagcaacc tgaagccaaa cgccaccagc
    28741 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc ttttttgtct
    28801 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg
    28861 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
    28921 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
    28981 aataattcac ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct
    29041 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
    29101 ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
    29161 caggtggctt atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa
    29221 aaagagcgtc aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
    29281 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
    29341 ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac
    29401 ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg
    29461 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
    29521 gctgaaagtg gtattccggc caatgatcgg ctggtgacat ttggcgaacc agacgaaagc
    29581 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
    29641 tgttataacg attcaatggc ggcgggcgcg atgggcgttc tcaatgataa tggtattgat
    29701 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
    29761 ccgcgcctga ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc tgccgaactg
    29821 gctttggcac tggcggataa tcgccctctc ccggaaatta ctaatgtatt tagtccgacg
    29881 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
    29941 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
    30001 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta
    30061 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc
    30121 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
    30181 gcccgattca cataacggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
    30241 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
    30301 cagatcgttg aatccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
    30361 ccgcacctga gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc
    30421 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
    30481 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
    30541 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc
    30601 acacacctgc tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
    30661 tagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
    30721 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
    30781 cggattaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
    30841 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
    30901 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
    30961 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
    31021 cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacttcaaa
    31081 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
    31141 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
    31201 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
    31261 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga
    31321 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
    31381 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
    31441 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg
    31501 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
    31561 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
    31621 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
    31681 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
    31741 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
    31801 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
    31861 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaattacat
    31921 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
    31981 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
    32041 ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg
    32101 tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
    32161 gcaccgcccg tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc
    32221 gaaactagtc acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca
    32281 gcatcctcgg catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc
    32341 accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg
    32401 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
    32461 tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg
    32521 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
    32581 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
    32641 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
    32701 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
    32761 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
    32821 tcgacgctat gtagcagcat ttgcgcagag tgaagcccac caagccgctg tttaatgcct
    32881 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
    32941 attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag
    33001 acaaacgcct caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca
    33061 gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt
    33121 tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtaatgc
    33181 ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat
    33241 caagcagtgt caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg
    33301 aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca
    33361 ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga
    33421 gccaagacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca
    33481 ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt
    33541 tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag
    33601 tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt
    33661 agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa
    33721 acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact
    33781 tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca
    33841 tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat
    33901 ttggcaggaa gaccaccaga ataatcagca gaactgctgg taaagcaaga acccccaaca
    33961 ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc
    34021 cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca
    34081 tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca
    34141 cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta
    34201 caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac
    34261 cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca
    34321 aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga
    34381 tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac
    34441 gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt
    34501 tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaattc acacaggtgt
    34561 caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta tgtgatggat
    34621 attccaattt taaaattaag ttgaattatt gagattatta ttaaccacct aattttaaag
    34681 cagataaaat tcataaagtt cattaattga taattgatat ggattatttc ataaccatga
    34741 tatggattat gat
//
LOCUS       DADKXM010000025        34651 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.025, whole genome shotgun sequence.
ACCESSION   DADKXM010000025 DADKXM010000000
VERSION     DADKXM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.025"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            36..2027
                     /locus_tag="JQR05_001250"
     CDS             36..2027
                     /locus_tag="JQR05_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5323458.1"
                     /translation="MDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQG
                     TPVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTR
                     TELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLAN
                     ADDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRT
                     NSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTRTQNNTDDSLRWDKQQ
                     SFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLS
                     FSLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGG
                     LNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLG
                     ASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSG
                     FVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVK
                     KFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGD
                     VTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2480..3112
                     /locus_tag="JQR05_001251"
                     /pseudo
     CDS             2480..3112
                     /locus_tag="JQR05_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(3200..4498)
                     /locus_tag="JQR05_001252"
     CDS             complement(3200..4498)
                     /locus_tag="JQR05_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5323459.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4981..6393
                     /gene="uxaC"
                     /locus_tag="JQR05_001253"
     CDS             4981..6393
                     /gene="uxaC"
                     /locus_tag="JQR05_001253"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY5323460.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6408..7895
                     /locus_tag="JQR05_001254"
     CDS             6408..7895
                     /locus_tag="JQR05_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY5323461.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYDLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7978..8529
                     /locus_tag="JQR05_001255"
     CDS             7978..8529
                     /locus_tag="JQR05_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY5323462.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8534..9778)
                     /gene="sstT"
                     /locus_tag="JQR05_001256"
     CDS             complement(8534..9778)
                     /gene="sstT"
                     /locus_tag="JQR05_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY5323463.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10374..11339)
                     /gene="alx"
                     /locus_tag="JQR05_001257"
     CDS             complement(10374..11339)
                     /gene="alx"
                     /locus_tag="JQR05_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY5323464.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLTLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11610..12626)
                     /locus_tag="JQR05_001258"
     CDS             complement(11610..12626)
                     /locus_tag="JQR05_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY5323465.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGALVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(12687..13379)
                     /locus_tag="JQR05_001259"
     CDS             complement(12687..13379)
                     /locus_tag="JQR05_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY5323466.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGHKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13456..13959)
                     /locus_tag="JQR05_001260"
     CDS             complement(13456..13959)
                     /locus_tag="JQR05_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5323467.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14044..15180
                     /gene="rlmG"
                     /locus_tag="JQR05_001261"
     CDS             14044..15180
                     /gene="rlmG"
                     /locus_tag="JQR05_001261"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY5323468.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15465..15779
                     /locus_tag="JQR05_001262"
     CDS             15465..15779
                     /locus_tag="JQR05_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY5323469.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15776..16192
                     /gene="higA"
                     /locus_tag="JQR05_001263"
     CDS             15776..16192
                     /gene="higA"
                     /locus_tag="JQR05_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY5323470.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16237..18255)
                     /locus_tag="JQR05_001264"
     CDS             complement(16237..18255)
                     /locus_tag="JQR05_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY5323471.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKITLQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18481..20832)
                     /gene="ygjK"
                     /locus_tag="JQR05_001265"
     CDS             complement(18481..20832)
                     /gene="ygjK"
                     /locus_tag="JQR05_001265"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY5323472.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMVHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQANYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20849..21920)
                     /gene="ygjJ"
                     /locus_tag="JQR05_001266"
                     /pseudo
     CDS             complement(20849..21920)
                     /gene="ygjJ"
                     /locus_tag="JQR05_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22054..23487)
                     /locus_tag="JQR05_001267"
     CDS             complement(22054..23487)
                     /locus_tag="JQR05_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5323473.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23550..23999)
                     /locus_tag="JQR05_001268"
     CDS             complement(23550..23999)
                     /locus_tag="JQR05_001268"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY5323474.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23996..27088)
                     /gene="ebgA"
                     /locus_tag="JQR05_001269"
     CDS             complement(23996..27088)
                     /gene="ebgA"
                     /locus_tag="JQR05_001269"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY5323475.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSH
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(27272..28255)
                     /gene="ebgR"
                     /locus_tag="JQR05_001270"
     CDS             complement(27272..28255)
                     /gene="ebgR"
                     /locus_tag="JQR05_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY5323476.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28474..28805
                     /locus_tag="JQR05_001271"
                     /pseudo
     CDS             28474..28805
                     /locus_tag="JQR05_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28847..30337)
                     /gene="ygjG"
                     /locus_tag="JQR05_001272"
     CDS             complement(28847..30337)
                     /gene="ygjG"
                     /locus_tag="JQR05_001272"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY5323477.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            30644..32164
                     /locus_tag="JQR05_001273"
     CDS             30644..32164
                     /locus_tag="JQR05_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY5323478.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQFGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(32212..32835)
                     /locus_tag="JQR05_001274"
     CDS             complement(32212..32835)
                     /locus_tag="JQR05_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY5323479.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33112..33876
                     /gene="nfeF"
                     /locus_tag="JQR05_001275"
     CDS             33112..33876
                     /gene="nfeF"
                     /locus_tag="JQR05_001275"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY5323480.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEKYDPQRVRAAAYWHAK"
     gene            complement(33930..34005)
                     /locus_tag="JQR05_001276"
     tRNA            complement(33930..34005)
                     /locus_tag="JQR05_001276"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33970..33972),aa:Ile,seq:cat)
     gene            34130..34636
                     /gene="mug"
                     /locus_tag="JQR05_001277"
     CDS             34130..34636
                     /gene="mug"
                     /locus_tag="JQR05_001277"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY5323481.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         8459 a         8923 c         9100 g         8169 t
ORIGIN      
        1 attttttacg tcgttattat gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg
       61 cacaaggcgg gaactttacc tttaactttc tgccactcgg tgcaatcgat gggatgcgta
      121 tcgggtcgac actgagctat ttaaaccagg cgcaaagcca gcaaggaacc ccagtaatgg
      181 ttctgctttc gcgcaattct cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt
      241 tttatctcaa tagtggttcg caatttattg ataccagttc ctttccgcca ggcagctaca
      301 gcgtggcatt aaaagtctac gaaaataacc aactcacccg caccgagctt gtgccgttta
      361 ccaaaaccgg cggtctgact gacggaaatg cgcaatggtt cttacaggca ggtaaaacta
      421 catcacaggt ttctgatgat gaaagttcag cttatcagct aggagtacgc ctgccattac
      481 atccgcaata tgagctctac gcagggctgg cgaatgccga tgatgtgagt gctttcgagt
      541 taggtaataa ctggacggca gatttaggcg gggtggggaa tcttgcaatc agcgccagcg
      601 tgttccgtaa cgatgacggc ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg
      661 gatggccgac gttgggcttt tatcggacca actctgacgg cgatgcttgt acaaccgaca
      721 gcagagagag ctataacgcc ttaagctgtt atgaaagtat ttccgcgaca gtttcactga
      781 attttgtcgg ctggaatatg atgctgggtt atacccgcac acaaaataac actgatgata
      841 gtttgcgttg ggataaacag cagagctttg aaaataacta tcttcgccag acaacagcgc
      901 aaagtatctc tgaaactgta caacttagcg cttcccgcgc tattgtgatg cgtgactgga
      961 ttttgagtac gtcagtcggt gttttccatc gtaatgacaa cggtggcgat aacgacgaca
     1021 acggcttgta cttatcgttt tcgttatctg acacgccaac gatggatagc aataacaaca
     1081 gccattcaac caatgtgtct acggattatc gttatagcga acaggatggc gatcaaacgt
     1141 catggcagtt atcgcatacc ttttataacg attcattcag ccataaagaa cttggcgtaa
     1201 ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt tgggatgggc
     1261 aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat catgatcatc
     1321 tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat ggcgttaatt
     1381 tgggagccag tggtacagac gatttgctgg gtgcggtact ggtggatgtg aaaggcttct
     1441 ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca ggcagccgaa
     1501 ctttgcagct tggtcaaagt gacagtgtgt tgttccctta tcctggattt cagtctggtt
     1561 ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac atcattaacg
     1621 gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg
     1681 ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc
     1741 cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga tttacacttg
     1801 aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc cttaagtgtc
     1861 cactgagtgt tgtaaagaaa cgcgccagca ttcgttacag cggagatgtt acttgtagtg
     1921 tggtgactta ttcacaatta ccggagtcga ttcaggttca ggcacagttg aaacagccta
     1981 aattacgtgg aaacgttcag acggcgcaaa gggaggttgc accatgagaa atcgattgat
     2041 tgcggcgata ttgggcttgt ttggcacgct cactggcgtt caggcagctc ctgacgtgac
     2101 cagtgaaatt acgtatgatt tggcatctgg tagagcggat tattacttct ggaaggatga
     2161 agcgtcggca ggaaataatg gatatatgtg gtatgaatgt tcgtatcctg acctccaaca
     2221 aacctgtaca gctaatggaa atatatcgac agtacaaatc tatttaactg aacaacgcag
     2281 tgggatgcgt tggccgataa aactcaaagg atttaaaaca gccattgtaa gtagcgatga
     2341 agcgccgcta ggatgcaagg ggggcaaagg gcttcagacg aatcttaagg attctaatag
     2401 atcttcatgt acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct
     2461 ctaccttgag cagacagaga tgaagaattt gccgattggt ggcgtctgga aggggaaagt
     2521 taaattacat tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct
     2581 cgaccccaac catattgacg tgttcttccc ggagttcgcc cacgccacgc caagggtgca
     2641 gttagacttg catccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat
     2701 gttggacatg tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct
     2761 caaagatgaa gggcgaccag ctgcagggcg cagagacggt tacttctcta tctatcgtca
     2821 gggagggacc accaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc
     2881 ggaaaccggt gggcaaattg atgtgcgcaa taatgaaaat atggtctgaa acagcattaa
     2941 cctgaaacgt gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc
     3001 gacacccttg actctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta
     3061 catgggcaaa ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa
     3121 accggacaaa cgcttttttc tatataaacg acgaagccgc ctgataaggc ggcttcgtcg
     3181 ttataactta cgttaaacgt taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca
     3241 cctcaatcgc cggtttgttc tgcaatacgg tccagataac cagcgcaccc agcaggtcaa
     3301 atactgccag aactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa
     3361 ccagcgcaaa cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg
     3421 ccacttcgtt acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct
     3481 ggtgggcaaa accaccgata cacagcagca taattgcgac atacgggttg gtgaacagac
     3541 cgatcatacc cgggccaatc atcagcacag cacccagcgt tacgaccatc ttacgggaaa
     3601 cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac
     3661 aaccgaggtc agcaaacaac atcggcatcc aggcgaacat cgcgatctct ttcaggttaa
     3721 agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggccggtt
     3781 ctgccaggaa acgcggcagt gcgatacccc agaactgacg gttacgcagg atctgaccaa
     3841 cggacatttt cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt
     3901 cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg
     3961 ccatcgccca gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca
     4021 tcacgattgc ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt
     4081 taaagtagcc tactgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca
     4141 gacccgccgg gatcatcgct gcttccgcgg caccgaccgc accacgagca acagccaggc
     4201 caccccagct acctgccagc gcggttgcac cacagaacac ggcccacagc acagcaaaca
     4261 ttgcataacc gattttcgta cccagcacat ccagtacata acctgctacc ggttgcatga
     4321 ccgtataagc agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta
     4381 actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca
     4441 cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt
     4501 aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga
     4561 agatgttagt tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga
     4621 aagtttcgtg ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga
     4681 atcggcaatt tgcattccgt cgacttatga tttgcgacgg cagaaagata acttgtcata
     4741 caaccttaaa aggtgagagc catcacaaat gtgcgaatat ttgtagggac attacctgac
     4801 gacagcgagg ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt
     4861 tttaactacg tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg
     4921 ataactttgt cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa
     4981 atgactccgt ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat
     5041 cacgactacg caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag
     5101 attgcggaag actatcgttt taaaaacctg tatgacatct ggttgaaggg cgatcactac
     5161 aaatggcgcg ctatgcgtac caacggtgtg gctgagcgtc tgtgtactgg tgatgcgtct
     5221 gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta
     5281 taccactgga cgcacctcga actgcgtcgt ccgttcggta tcactggcaa attgctttct
     5341 ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc
     5401 tccgcgcgcg gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg
     5461 atcgattctc tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg
     5521 ctgccgagct ggcgtccgga caaagccttc aatatcgaac aggcgacctt taacgactac
     5581 atggcgaagc tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact
     5641 gccttgacca aacgtctgga tcacttcgct gctcacggct gtaaagtgtc tgaccatgcg
     5701 ctggacgtgg tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc
     5761 cgtctggctg gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg
     5821 gtgttccttg gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg
     5881 ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc
     5941 atcaacgacc gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa
     6001 aacctgctgc cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc
     6061 accatgatcg gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc
     6121 tggtggttta acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc
     6181 ggtctgctga gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc
     6241 cgtcacgaat acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc
     6301 gaagcaccgg cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat
     6361 gcgcgtgact acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca
     6421 agatccatgc gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag
     6481 tcagtgtcga taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg
     6541 cgttaacgga tatcgcaaaa ggggccaacg tcatcaaata tgacctgccg attggttatg
     6601 cattggcgga tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga
     6661 gcgatctgga tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag
     6721 atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt
     6781 ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc
     6841 tgaaagagac caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg
     6901 gctgctcaca actgggcgac gatcacatca acacccgcac catgctgcaa aacatggtgc
     6961 gccacccgaa cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg
     7021 ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc
     7081 aacagcagga tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga
     7141 tgcgcaacga taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg
     7201 gtggttctga cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact
     7261 acgtgattgc taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag
     7321 agcagttgct gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg
     7381 tcaacgactt caaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac
     7441 cggggaacaa agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga
     7501 aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag
     7561 ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg
     7621 cgggctgcca tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc
     7681 cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact
     7741 ttgacgcggg tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg
     7801 acaccatcgt tgagtttgcc aacggtaagc aaacctgcaa cgagcgtaac gacttccgcg
     7861 aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa
     7921 acggcgtttc ataatgtgaa gcgccgtttg cttttccata cctgctaagg atttatcatg
     7981 accgcgtatt ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca
     8041 tttttcaatc gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt
     8101 ggcgtacatt tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc
     8161 attcgtacat tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg
     8221 ctgactggcg gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc
     8281 ggtacgattg tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg
     8341 atgtggtttt caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc
     8401 acgatgattg agggtagttt tctgcttatg aatggcctga atatcattcg tttctggcgg
     8461 atgcagaaaa ggggaattga tccgtttaaa gtagagaaaa cccctcccgc catagacgaa
     8521 aggggttaaa caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc
     8581 aagctgccgc agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct
     8641 gcaatacgcc gataataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac
     8701 cgaacatatt acaggccagt gggatcagca gcaaagaacc ccccgccacg ccggatgcgc
     8761 cacaggcaca cagagaagcc actacgctca acagcagcgc tgtgggcaga tcgaccggaa
     8821 tacccagcgt attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca
     8881 tattgatggt ggctcccagc ggaatagaaa ctgaataggt atcgcgatcc agattcagct
     8941 tttcacacag cgccatattc accggaatgt tcgctgcaga gctgcgggtg aagaaggcat
     9001 acacaccgct ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc
     9061 accacaccag caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga
     9121 ccagcagttg cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa
     9181 ccagcccaaa aataccgatc ggtgcgaagc gaatgaccag tttcaccata aaggtaacgg
     9241 cattcgacat atcattgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaagc
     9301 cgaggccgat cgcccacacc agaatcccga tgtagttacc tttcagcagg gcgtcgatgg
     9361 ggttggaaac catgctcatt accagtccgc gcatcacttc gacaatgcct gacggcggcg
     9421 aaatatcacc cgcgctactg gacaagtgca gggtagaagg gaaggcaaag ctgaagacta
     9481 ctgcggccag agctgcagag aaggtgccca gcagatagag gaacaaaata ggacggatat
     9541 tggttttctg cccgtgctgg tggttagcaa tagatgccat taccagcatc aacaccagga
     9601 tgggggcaac agctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg
     9661 ccgcgggttt tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt
     9721 ttaccaggct gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt
     9781 ttcgatcctt tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag
     9841 gatgtaacaa agtgtttgca aggtcgagta taaggaaagt tacagtttcg ggaagcgaaa
     9901 aatgctggat tttaaagaga ggtcatgttt tgcaacttaa tggttgcatt tatttaacat
     9961 aaacagaggg aaggactggc tactaaggct gattagataa catatgggca aaactatttg
    10021 gaatatgaga acggttgcgc tcctttaacg tgttatgcca tataaagtca ctggcgcagg
    10081 gagcgcgcag ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa
    10141 aatcgccgct gcgcggttcc ctcagcctat cccttgcggc taccgggtcg ggcgcaaggt
    10201 aacgtccctg taagatgcgc cctcgccccg gcctccaggg aacgcttcgg cgattttgac
    10261 gccaccaaca cccgtgcggt ctttttgtaa agagctataa gccgattaca taattaatag
    10321 ctcacctttg tctggaatga gttatctcta ggaatggctt aatatacagc gaatcattct
    10381 ccccgctgct tatcatgccg ataattcacc caggcgttga taataaacgt catcaccaga
    10441 atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc gacaatcagc
    10501 atcttgatac cgataaacac cagaatcacc gccaggccat atttgagcat cgagaaacgc
    10561 tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggattgc aaacaggttt
    10621 gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat gctatccacg
    10681 gcaaaaatca cgtcgctcaa ttccaccaga atcagtacca gcgtcagcgg tgtggcgtac
    10741 agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa
    10801 tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg
    10861 agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag gatccagtcg
    10921 aactgcgaaa tcagccagct gcctgtgaag atcatgatgg tacgcagaac aatcgccccg
    10981 agcacaccat acaccagcac gcgacgttgt aatgccgccg gaacggagaa atagctgaac
    11041 aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag ataacccgtg
    11101 agaaaggcca gggcctgtgg atcggcaacg gcgcgacctt cggtttgcac cagataccac
    11161 cagaaagcgg cgttaaacag taacgacagc gtcacccaga ccagcgacca ggccgcagcc
    11221 tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc gatagccagc
    11281 ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac agtattcata
    11341 gaagttcctt acacataaaa aaacggccaa cgtcggaaga cgtcagccgc tgctttttat
    11401 gcatagacct cgccttccgg caaggtctca cttacaacaa aaaaggaata tgtctgcgta
    11461 ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat gacgatcgac
    11521 cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagaccatt cagtcagtca
    11581 atggtctgtt acataccttt tacactgttt tactttggca agtatagttt tacgctatct
    11641 gccggaaaaa tcaccccagt ctggcggcgg atctcggtca gcagtttggc tgtgatgcga
    11701 ctgaccgcca gccctggatg atccaccaga tgctcatcca ccagggtagc gaacagctct
    11761 gcttcgtaga gcatggtatt aatatgctgc ggctgggtga gatcctgcat ctggctgcca
    11821 cgcggcacaa agcagacttt ctggcattca gacagttttt caatcaccag cgctcccgct
    11881 tcgccctgaa tctcgcttcc caggacagaa tcactgacct ttgagtgctg caaggtgacg
    11941 ctgaaatcac cgtaatccat caccaccacg ccgtgggcgt caacgccgct tgccagcaaa
    12001 ctcgccgttg cctgcacgct tttcggctca ccaaataacg ccaccgccga cgccaggcag
    12061 taaaagccga tatccataat tgaaccgtta gagaatgccg gattaaaggt gttgggattc
    12121 tcaccgtcca ggtaacgttg ataccgcgag gaatactggc aatagttgaa aaagacttta
    12181 cgcagtttgc ctgctttcgg cagcgcctgg cgcaacaaat gaaagttcgg caggcaggcg
    12241 gttttaaatg cctcaaacag caccacctga ttttcgcgcg cacaggcaat ggcggcatcc
    12301 acttccgcca gattcgacgc cagcggtttt tcgcaaatca catgaatttt atggctaagg
    12361 aaaagttgtg tctgggaaaa gtgcagggaa ttcgggctgg caatatacac cgcgtcaatg
    12421 gcatcgcttt ccgccatcgc ttccagcgac gtaaacagat gctcgacaga aaaatcattg
    12481 gcgaagtgct gagcctgctc aaggctgcgg gaatatacgg cggttaactt gtatttaccg
    12541 ctctcatggg cggcctcgac gaactggcga gtgatccagt tcgtaccaat cacagcgaaa
    12601 cgtatcataa cgcgtggata ctccataaag ggattctgtc gtcaatgtag cacgcctttt
    12661 tcatcttcga cgtgcgcaga tacgcattat tctttcgccg gacagccatg ctcactaaac
    12721 ggcccaatca tgaccgatgg cccgagatat ttcggctggc gatggaggat ttttgcgtca
    12781 atcaccgcgc tgacacgagc gagtttctcc cgcagttgag tataaaaacc gtgcttcata
    12841 ttgaggtctg gcgcgttaaa cccgatagcg tcgatgccgt actgttttgc cagccagatg
    12901 gcgcgttggt tatgaaattc ttgcgagata atggtgatat ggttttcccc aaacaccttt
    12961 ttggcacgca ctaccgaatc cagcgttgag aatccggcat agtcgcaaaa gatcactttc
    13021 gccggtacgc ctttagcgat caaagcctgc tgcatacctg atgcttcatc gtaattttta
    13081 tgtccgttat cgccactcac cagtagccat ttcacttttc cggcgtggta caacgctgct
    13141 gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tcgcccccag caataaaccc
    13201 acgttacgcg ccgggacggc gttgacatcg ccccaggtca gctgcttgct ggcgttgacc
    13261 ataacgcgat cggcgataaa aatggtcgct cctgccacaa gtaacagtaa gcaggcaatc
    13321 tttagagtcc ggcgagaaaa acaaatacgg agaagaaggc gggcaaatgc gcgcagcatg
    13381 gtcgttatcc ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa
    13441 cgcctgacat cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg
    13501 ggtgtcgaac tcaagctggt ggtactgcgg ctccatatgg cagcacaatt gatagaacgc
    13561 tttgttatgc tctttctcct tcaggtgcgc cagctcgtgc accacgatca tgcgcaaaaa
    13621 cggttccggc gcgttgcgaa acacggtagc gacgcggatc tctgcttttg ctttcagctt
    13681 gccgccctgc acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat
    13741 cttattgtca tacatcactt tattgatcgg cggtgcatta cgcagaaact gatttttcag
    13801 atcctgagta tattgccaga gagctttatc ggtggcgtag tcgtgtgttc ccggatagcg
    13861 ttttgccagc acatcgccca gacgctgttc gttaatcagt gttcgcacct gcgaaagcag
    13921 ctgctcggga taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa
    13981 aaagggtata ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg
    14041 ccgatgagcc acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg
    14101 gatgacgtta acccgctaca ggcgtgggaa gcggcagatg aatatttgct gcaacagttg
    14161 gacgacacag aaatccccgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc
    14221 tgcgcgctgg cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg
    14281 acgcgcgaga atttacgcct aaacgggatt gatgagtcga gcgtgaagtt tctcgacagc
    14341 accgccgact acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca
    14401 ttgctggaac agcaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt
    14461 gccggtgcca aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc
    14521 ggcccgacca ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat
    14581 gaaccgccgc tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg
    14641 actatccata accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc
    14701 tttatgcaac atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat
    14761 ggcgttattg gtctgacgct gcttgataaa aacccgcagg cgaaagtggt gtttgtcgat
    14821 gaatcgccga tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg
    14881 ttggatcgca gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt
    14941 aatgctgtgc tctgcaaccc tccgtttcac caacaacatg cgctgaccga taacgtcgcc
    15001 tgggagatgt tccaccacgc ccgccgctgc ctgaaaatca acggcgagct gtatatcgtt
    15061 gccaaccgcc atctggatta cttccataaa ctgaagaaaa ttttcggcaa ctgcaccact
    15121 attgcgacga ataataaatt tgtggtgctg aaagcggtga agctgggacg tcgtcggtaa
    15181 gattgggagt tacctgccgg atgcggcgta aacgccttat ccggcctacg ttcagcacgt
    15241 tacccccagg ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg ctcagttctg
    15301 gaatcgcgct tccataaaac aaaaaatccc tgtcaccatc ctctacattc tcttgtttag
    15361 cgtttttcta cgtttattct tccgtcacac agataaattc atccgttgcc aatctgtcaa
    15421 cggatgttat catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc
    15481 aaaaagcatt gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc
    15541 tggggaacac gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc
    15601 catctctgga taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg
    15661 aattacgtgt tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta
    15721 tgacgcataa agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat
    15781 tgcgattgcc gacatcttgc aagcaggaga aaagctaact gctgtggcac cttttctggc
    15841 gggtattcag aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct
    15901 caacgatcct gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga
    15961 atcagcgccc gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc
    16021 cgtgattcgt acccttatgg atcaatatgg tttaaccctt tccgatctgc cggaaattgg
    16081 cagtaaatct atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc
    16141 taaaaaattg gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt
    16201 gccggatgcg ttgcatccgg cgcttcaaat aacgatttaa atctccagcg ccagccgtgt
    16261 tccctgggca attgcccgtc gtgcgtccag ctccatagcc acatcgcagc cgccaattaa
    16321 atgcacagtt ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc
    16381 tgcgcagatc accacattgt ccactgctaa tacctgcgtt tcgccgttga tcaccacatg
    16441 cagcccgtca tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga
    16501 gagcagggtg gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct
    16561 ggctttgcgc tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg
    16621 gcttaagcca ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc
    16681 gatattctgg ctggtggatt cgcccggctg acttaaatac atcgccgtat caaaaccaat
    16741 cccgccacaa ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatc
    16801 gagataactc aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat
    16861 cccactggcg aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt
    16921 gagtttcagc gtcacgcccg tcacttcgat catccggcgg tagtagcgca gcgtttcgta
    16981 aaactcctct ttgccgggga tctgtttggc gatattaaac tgcccgccga tctcgctgtg
    17041 cgcatcaaac aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc
    17101 agcaggtcca gcgccgacca ccgccagatt ttttttctgc acggcgggaa ggattggcat
    17161 tttggtttcg tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat
    17221 ctgatcgagg caggcctggt tgcagccaat acaagtgttg atctcatcgg ctcgccctga
    17281 ttgcgctttt gacagcagtt ccgcatccgc gagaaacggt cgcgccatcg atactatatc
    17341 ggcatcgccg cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt
    17401 taccagcggc agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg
    17461 cggcacgggc gtggcgatgg tcggaatccg cgcttcgtgc cagcctattc cggtgttgat
    17521 aatggtcgcg cccgccgctt caatggcctg cgccagttct accgtttcgg caaaagtgcc
    17581 gccgccttcg accaggtcga gcatcgacaa ccggtaaata ataatgaagt cgttgccgac
    17641 gcgttcgcgc accgtacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc
    17701 gccccactgg tcactacgct gattggtgcg cagcgtcaga aattcgttga tcaaataccc
    17761 ttcagagccc atcacttcga cgccatcgta tccggcctcc cgcgccagtt gcgcgcagcg
    17821 ggcgaaatcg tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg
    17881 gttgatgggg gcctgcaatg cggacggtgc gaccagatgc ggttggtagc tgtagcgccc
    17941 ggtatgcaaa atttgcaggg tgattttgcc gccttcctga tgtacggctt cggtaatggt
    18001 gcgatggtgt gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc
    18061 tgttaaatct ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc
    18121 ggcataaaac gcagccagcc gttcagcacc gtccgggtat tcctccagcc cggtgtgcat
    18181 tgaacccatc aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag
    18241 cgacgggtag ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt
    18301 tctaatttag ccgtcgatgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt
    18361 caaaaagaat acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt tatccggctt
    18421 gggggattgc acatgtaggg cggataaggc gttcacgccg catccgccag ccgttgcaaa
    18481 ttactgctta cggaaaaaat cgttatacaa catatacaaa tgagcggcac tccaggagaa
    18541 atttggtgcg ccttgctgcg cgcctgtcag cgggttgtaa ttctcctgaa ttgggccatc
    18601 ggcggttaat cctttggcgt gcctgaagaa cgtatccgcc agtttcaggg catcatcacg
    18661 ataaccgtaa cgctccatcc ctttcagacc aaaccagaac tgatccaccc atacgcgccc
    18721 gcgccagtag atatcagcgc caaaagccgg attggttaac gccgccgttc ccagcggaac
    18781 aaaggtgttg aactctttag gatcgagcat caccttcacc accgcgtcgg cattggcctg
    18841 cgttgccgca ccgttaaaca gcggcgacca gccttccggc cctttaccgc gctcaacaat
    18901 cggtttgccc gcgcagccgt tcgccagtgg tttatcttca atacgtacgt catagtagaa
    18961 ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg
    19021 gcgatagcgt ttggcctctt ctggcttacc gaggatggtt gccatctccg ccagataatg
    19081 gttatcgctg tacatatagt tggcctgatc caccgactcc tgcaatagcg agtagcccag
    19141 caacgttccg tcctgactgc ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc
    19201 gccattagcg acatatttat ccaactgttc tttgtcgata aaaccaaaga cggcggcatc
    19261 atcacgcccc gattcccacg acgcagcgac ctgtgccgga atttccagac tgtcgtactg
    19321 gcctttctcc accacgcggg cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc
    19381 ttttttcacc gtaaacagca tctcgccgct ctcggtgttg tgggctttgt cgcgggtcgc
    19441 gccatattcc ggcaccccgt tgccgttatg atcgcggtta cgtaaccacc agtcgtgata
    19501 ggccaccagt ttcgggtaca tctctgccaa ccaggcttta tcctgggtga cgttgtatac
    19561 ttccatcacc gaccaggcgg caaggctggg tttggtatta cgttcgttcc agttgccgcc
    19621 atcgccacca cgctcggggc taaggttcca cgctatcagg tcggggacaa agcccacatc
    19681 ctgcggacgc acgctgtcgc caggctggat ctgccaggag aagaccgcgc ggatattctc
    19741 tttggcgata tccggattga aatgcaccat cgcaaacgcc tgcttccagg tatcccacgg
    19801 ccaggtctga ttgccggaga accagcgtcc ggtcaccgat ggcgtgacgg tgttataatt
    19861 caccgcaccg cccggtgagc gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac
    19921 gcgcgtctgt tccggcgtcg catccggatt ggttaaccct ttcttcaggt attcttccca
    19981 gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc agaatatcgc ggatctgcat
    20041 ttgctctttg ctaacttcct gagcggtcag cagatgggaa taggtggtgt agagcgtggt
    20101 cgaaccgttg atatgcgcct tactggtaaa gcgattaccg ttgatttcag tctgcaccgg
    20161 cagggattta tgcacctgat attccgattc gccggaggtc agcagatccc aggtggcgcg
    20221 cactttgcca aaggtgactt tcaggccatc acgggtggcg ctgattttgc gctgatagtc
    20281 agggtattcg ccagcaatgg ttttatcgga aagcggtttc ccttctttcg cttccagttt
    20341 ttccagcagt tcgccatccc acaccagatc cagcggttta ttgctgatga ttttagtttc
    20401 cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt tcgacctgca catcttttgc
    20461 tgtcagtttt tgcaccagcg cgccgggaat actgtatgcc tccagcgtga agttgacttt
    20521 cttgccgtct tgccagacag tcaggcggtc gaaattgctg gccataaagt tgatgtattc
    20581 ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg ttcgggccgt ctggcaacag
    20641 atgaccatgc caggctccga gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc
    20701 gtaatccttc atgtactgcg gcgcaccagt acggttaatc acgtttttat aattgtcggc
    20761 gttagcggca tgagcggaaa aacttatcag cagagcgcag gttactggcg ttaaaatagt
    20821 tttgattttc atgaaattac agtccttatt accagtagaa atattgcatc aggaagattt
    20881 ggtcgctggg atcgttagag gcatttttca tgtagaagcg gctatcaaaa gccgtggcga
    20941 aacgtccgcc gccatattcg taccagatac caaactgcat gaaagaggtg gtttcatcgt
    21001 cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt ggagagataa acgcccttta
    21061 acgcttcata catggcgctc accatcatcc cgctttcact tcccggtacg tccagcccgt
    21121 cggcacgtcc ggtatggtgc caggcgcgag cggcaaagtt aggggtaaac aggcccgtga
    21181 gatagagttg tcctgtgccg tcggtaattt ccagaccatt catccaggtg atgccttcgg
    21241 caaggtcata ctggatgtaa ccgttcatcc attgccggat aggtgtattt gtcgtcgtag
    21301 cgatcgtact tgccaaaatg tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg
    21361 gcggtgacgc tataacgaaa gttgccgctt aagttctgta ccttcagcgc ggtcataatg
    21421 tcgcgggtat tgggaatgac gtagcccaaa tcgggtgtga agtcgcccca ccattgcagg
    21481 tcatccagcg aggaatcctg acgcagactg agcatgattt cggtgccatc attggtacga
    21541 taaccgccat agaaacggtt aatcccacct tcgaaaccac cccagctatg gtcaggcacc
    21601 caggcattac cgtcgtggtc ggcctgcacg gtccagcctt cgccgtaaat caggccgaac
    21661 caggagccgt acttcatctc caggccacct tcaatatagg tgccgtcgct gtatttatgt
    21721 ttattctcac cctctaaatc catatatcca ccaagcccta attcgccgta aaaacgaaat
    21781 gaaggagaag atgtcgaggt aattgcgggt gtttttgtgt cgtcatgttc tgccggacgt
    21841 gcttcttctc ctgccgcaga aaaggcgtag caaaacggca gagcaagtaa tgctctgcat
    21901 ggtgcagtga ttaatttcat tgtgttaatc catgagctaa agaaaaagta cagcctttta
    21961 tcgttgtaat tataaaaata taaaggcaga aactattttc cctctcctca tccaggaaga
    22021 gggaaaatgc ctgctcattt tttattagtc tgcttaatca acattgctgg ctggcgtcgc
    22081 ttcaatatgg cgctcttcag ccgttaatcc ctttatatat ttactgtatt tccaccacgc
    22141 aaagccgagg aagataacaa taccgccgac gttataaaaa atgatggtca gaatattcgc
    22201 gccagtcggg aatgtcgaag cgacaaatcc tacggcaaat atcgcaatca gcattgaaac
    22261 aacgataata ccggtgcggc gggagcccat acggaaatcg cgtggcaggt gatctaattt
    22321 ggcgcgtaaa ttcaggtaag ccagcatgat gaataacggc ggaagcatgg acgctgcggc
    22381 ggtcatatta ataatggtat tcatcagatc ctgcacggta ttggaaccga gcatcgggat
    22441 aatcatcagc gggatgacga tcagaaactg gatccatgct gcgcgcgccg gaacgccgtt
    22501 ttcgttaagt tcgacggttt tcttaccaaa gatgccttcc gggatttcgg agaagaaaat
    22561 tttcaccggc gttgcggtcc acatcagcag ggaaccgaac attgcggtaa aggacaccag
    22621 accgacaaag cgattcatca acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa
    22681 tacctgcacc gatccgccgg taaatttcaa ctctttgctg ctgacgaaga cgttaatcag
    22741 caccgaggag acggaataca gtacgccgat aaaaatcccg gcgaggatga tcactttaac
    22801 gaacgatttc gaaccgcctt tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc
    22861 tgcggccata aagatccagg tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat
    22921 cgcgtcaacg gtgatcggat cggcaggctg tacgccgcca accagtgccg tacctgccag
    22981 taaaatgtag gagagcgtta acagcagcat cagcgttgaa gtgacggagg taattggccc
    23041 cagcattttc gccccgttgg tggaaaccca ggtggagaag gcgaacagca ccatactgat
    23101 aacggtggtg gcaaccggcg tcataatata ttcgtagccg aggaaggcat acgaagcata
    23161 agcaataacg cgcggcaaca gcgaggtgaa aaagaacaga tttacgaacc agtaagtgta
    23221 ggcagtaata aatgcccaac gtccgcccag cgaacttttt acccacgcgt agacaccggc
    23281 ttctgagttt ttatttaacg aaacaaattc tgcgatgatc agacagaagg gaataaaata
    23341 aaaaatcgtc gcgaggaaaa acatcggtgc cgaggccagt ccaagctcaa tattattatt
    23401 gataacgttg ttaaagctgt aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc
    23461 gattgtatta cgtttggtat cagacataac acatctccga aaatgtgtag ggtagcggtg
    23521 gcggcaaact ccttttgccg ccgtagttgt tatttgttat ggaaataacc atcttcgatg
    23581 gtgactttga gaaccacttt tttgaccgcg ttattgcaga taaaccgata cgcttcatgg
    23641 atatcgcaaa taacgatttg cccttcgtgg acctctacgg tttctccgca gccttttaaa
    23701 tattcacggt cagtttcatc acgataatat tccactacct gtaatgtctc tttcggcgca
    23761 tattcaattt tttgctgccc ttgcaggtag taatgcactt caaaatagcg tcgatgcccg
    23821 gtaaatagcg catcggtcgc ggagtcattt tccacacggt aggtcaatga atcaccgatg
    23881 gagtgggcga cgccaggctg aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc
    23941 cacttcttgc cagaggcgta aatctggcgg aactgttcta agttatcgat gatcctcatt
    24001 gcttattctc gctgtgcaaa ttcgtggaaa agaaccctgc gccgaactca tacgacgcca
    24061 ggctttgcgc ggtagcttct ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt
    24121 cacggaacca gacgcgccag gagtccagca cctcgctgcc ccaggagttg gagccgaggc
    24181 caagtagctg gtggtcgagg ttcagggtga tgtcatcact gcgctgcagc tcgttacagt
    24241 gctgggcagc gtggatgttt tcctgggtat agtgccaggc gctgaagtta attgggcgct
    24301 gcggaaccac cagcagaccg ttgccgtggc ggttagtcag tgccgtccag cggacatgct
    24361 gacggttgcc gttgttctgc gggaagggat agttctcgaa catggcatcg acggtgctgc
    24421 gccagatatc gatgatgtta gcctgctggc tgtcggcgta gttttcgccc ggtccacgac
    24481 cgtaatacgc cacctgatcg tattcgccgt taatccccat ggtgaaaccg atgcacggaa
    24541 tgatgtgcgg atagtcgccg taacgctcgc cggaaagcgc cacgttaacc tggccatcgg
    24601 cagcgatgcg ccagatgtag gtacagcgca tcccgaagtc aaacaccggc ggtgcaataa
    24661 ctgtgcggct gatgatcaac acttcaccat cgctctgttc tacggcaaag tcgcgcagat
    24721 gttcctgcat gatctgcaaa tgattcggct gccacaaccc ttcgtactcc tgcttgtggt
    24781 tgtcgatcat cggcttgaag aagttgatct ttggctcgcg ggtcagcagc gactcgccat
    24841 ttacctgcca ggatgtcggt ttgccactca tttttgagaa ggtgatcgcg aagttgtagc
    24901 cgcgaacggt gcagctcaaa cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg
    24961 cgaaaggcac tggctgcgcg gtgttttcct tcagcgggaa ctgataagtg gcgatagaat
    25021 gcccggcttc gctgtagcgg gtgtgggaat ctttggtcac cgtaatgttg aggaacgctt
    25081 cgcgggcgtc cagctgcggc agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt
    25141 cgcgcagttt aatctgctgc gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca
    25201 gggtgtagtc atcaagcgtg gtaaaccaca gtttattttc gactttcaac tcgccgcgag
    25261 tcagatcccg cgcgtggatt tttaccggcg cgataacctg tttgtactct ttcaggcccg
    25321 atcccggcgt ctgatcggaa tagatcaaac catcaagaca gaagttatag ttgttgggat
    25381 agtcgccgta gtcgccgccg aatttatacc agacattgcc gtggtcgtcc tgtgcctgga
    25441 tcccgtggtc gcaccactcc cagacataat gaccctgaat gcaatcgtgc ttatagaaga
    25501 cgttctggta ctccgtcagc ccgcccggtc cgttccccat cgcatgagca tattcacaga
    25561 tgatgcgcgg cttcggatgc gggtattcac caaactcatt catcagcggc acgcgggtgt
    25621 acatggtgga aataatatcg accacttcag catcgcgatc ttcttcgtaa tgcaccagtc
    25681 gcgtgtcatc cagcgctttc gccgcatggt acatcgcgcg gatgttacag ccatagccgg
    25741 attcattgcc cagcgaccag atgatgatcg acggatggtt tttctgtgcg tggatatggc
    25801 gaacaatgcg ctcgacgtag actttttccc actgcggatc gtcggtaata cggctgatat
    25861 cgccgacatt agcaaagccg tgcgattcga cgtcggtttc cgccatcaca aacaggccgt
    25921 agatatcaca cagttcgtaa aaacgcggat cgttcgggta gtgagcggta cgcacggagt
    25981 tgatattgtg ctgcttcatc aactggagat ctttctcgac gcgatccatt ccaacggcgc
    26041 ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg cagcatcaca taacggttat
    26101 tgatccagaa cagaccgtcg cgcactttga tatcacggaa gccaacgcgt tgtggcacca
    26161 cttccagaac gttgccgttg gcgtctttca gcgtcatgac cagatggtaa agataagggg
    26221 attctgctga ccattgctgc ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt
    26281 tttcaattgc caaatgatca atggcgctgc tgtgcaccac gcgttcgcca tcaaacaggg
    26341 tatattccag cgtcgtgacg acaggggagg cggcgagatt ttccagcacc acttcgcagg
    26401 aaagcgtggc atcgcaatag gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat
    26461 gcgttaggtg ttttccgacc agataaacat cgcggaagat ccccgccgac caccacatat
    26521 cctggtcttc cacgtaggta gagtccgccc actgcatcac gcgcacacac aacaggttgt
    26581 cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt caggcgactg cccttgctga
    26641 aacccacata ctgaccgtta acgtagactt caaaataggt ttcgacgccg tcaaatttaa
    26701 tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa aatacgttga taggcaccgg
    26761 ttgggttatc gctggggaca aacggcacat cgatggggaa cggaaaacct tcgtcggtat
    26821 attgcagttt gccgtgacct tccatttgcc acatggcggg gacggtaata tgcccccagt
    26881 cagccattaa ctcagaggtg aaggcttctg gtacttgcag cggatggtca aaaaagtgga
    26941 aattccactg accgcttaag ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg
    27001 cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg
    27061 tgagctgaat gttttcccag cgattcatag ggcctcccgg tagagagaaa tcggaaccat
    27121 tcatggtgat ggtagtttgt gtaattgctg gggagtttag taaaattttt actaaaaagt
    27181 agcaggtaaa tgcatttact ttaactggat cacgaaaata aacggcagca ctgtccggca
    27241 aaaatgattg ccggacagtg tgtaagggga tttaacgggt cgtgccgcgc agttttaatt
    27301 tgctgggaac gaagactagc agcggcagcg cgcgaccatc gcgggctttt tcatacacca
    27361 ggttaacgcc ctgacttccc atcatctcgg aatggatgcg cacggtggag agcggcggaa
    27421 aggtaaatcg cgcggtgggg atatcgttaa cgctgataag cgaaatatcc tgtgggatgt
    27481 tcaggccacg ttcatgaatt gcccgcagta cgccgatagc aatggaatcg gaagcaacaa
    27541 acagtgcctt cggatagtct tcccgcgcca gcatttgttt tgccagttca taacccgacg
    27601 aactggaaaa accgccgcgc cagatatcct cttcgcgtac cacttgtttc agtcggccat
    27661 attccgcaaa ggcgacctca cgaatatccg ccttgccagg ctcatcttca ccgccaataa
    27721 aaccaatacg attaacgccc tggttgatat agaagtcgat gatttcttta ctgatgcgtg
    27781 ccagatcgat atccaccgca tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga
    27841 tattgtcggt caacgcgctg gcagcggcgc gcagggcggg cgtgggtttg ccgacaatta
    27901 aaataccggt gacgttttta atgtctggta atccgttgtg ttcataacag ttggtcagtt
    27961 cgatcgccag cttttcgcac tgagtttcaa tgccgtggcg gatcgccaga tagtaaggat
    28021 cgttgatctc cagctcctgc tggtagctgt agatagccag aatatggtgt tggttgactg
    28081 cgcctgtctg tagtttacgg gcgctactgg tcttgtactc cagcttttcg gcgatctcga
    28141 gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg atcgtcattt aagaccctgg
    28201 atactgtcgc cagggatacg ccagcttcga ttgcgatgtc ttttaatgtt gccatgttta
    28261 ccttttttac tttccctgat gtgcgccccc attgtagagg aagtgcttat cgggtcacct
    28321 gttttcagta aataaagcgc gtttctttga cgctgtaacg gtaacacagc ggcttaggaa
    28381 tgtgatcacg cctgcattct ccatttttag cctacaggat tgagaaaaac ttttgctata
    28441 gtttagtaaa attttacccg aagggaggaa gcaatggaaa ccgtggctta cgctgatttt
    28501 gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga aacgccatga aaacgccgac
    28561 aagctgtaca tcgtacaggt tgatgtgggg aaaaaacact gcaaaccgtg accagcctgg
    28621 tgccgtacta cagcgaagag gagctgatgg ggaaaacggt ggtggtctta tgcaatctgc
    28681 aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg
    28741 acggcagtga aagcgtgttg ttaacgccgg agcggatgat gcctgcgggc gtacgcgtcg
    28801 tataaaaaga tcggatggcg acgtcgtatc gccatccgat ttgatattac gcttcttcga
    28861 cacttactcg catggccgcc agcgccttac gcgccgcttt gatcaccagt tcacactgtt
    28921 caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt ggcgttattg agcgttccgg
    28981 ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt atagccgatt tcgttatcaa
    29041 caaactcaat cgccatcaac atccctttac cacgcgcttc ctgtaccaga tcgggatatt
    29101 cccgcgccag ttgacggaaa ccatccagca acatatcgcc tttttgctca gcctgagccg
    29161 gtaagttctg ctccagcaac acattgatgg tcgccagcgc cgccgcacag gccagcgggt
    29221 tgccgccaaa ggtggtggta tgcaggaatg ggttgtcgaa cagaactgaa aacacctctt
    29281 cagtggcgat ggtcgcgcca atcggcatca cgccgccgcc gagggctttt gccagacaca
    29341 gaatgtccgg ctgaacgttc tcatgttcgc aggcaaacat cttgcccgtg cgccccatgc
    29401 ccgtttgtac ttcatcgagg atcatcagtg cgccgaactc atcgcacagc ttacgcacgg
    29461 cggtgagata gcccggcggc ggcagaatta cgccaccttc gccctgaatc ggttcgagga
    29521 tcaccgcagc cacatcatca ccggtttttt tgcactcatt aagagccgtg cgcatggctt
    29581 cgatattgcc aaacggcaca tgacggaacc ctggcagcag tggcataaac ggtttgcgga
    29641 aggtcgattt cgccgtggcc gacagcgcgc caagtgattt accgtggaac gcgccgctgg
    29701 tggcaataaa agtaaatttg ccgcgcggcg actgataagc cttcgccagc ttcagcgccg
    29761 cttcaacgga ctcggtgccg ctattacaga agaagctgta tttcagttta ccgggcgtta
    29821 gcgcagcaag ggttttcgcc agcatagccc gtaacggatc gagtagctcc tggctgtgca
    29881 gtggttgttt cgcaagttga ttctgtacgg cggaaaccac aactggatta cggtgcccca
    29941 cgttgaaaat tccaaaacct cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa
    30001 gcgtatttaa acctcccgct tgccactcta cggctccgta atccccgccg gcggtaacag
    30061 atttgcgata ctctaaaaac cccggattga catgctcttt gaagtattca atcacctctc
    30121 ggttaagtgc tttcatctcc tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt
    30181 gggcgctgca cgctaaagcc gatgcgctcg aaggtaacct gttcaaaata tgctccggag
    30241 gctcgcgtat cacatgatac cgatttaagt attgcaggga ttgcgccact ccggctgcga
    30301 tcgcgaaaat cgggataaac acaaatgcgg ttatcatttg tgcaatattt aatgctatcc
    30361 gttaacattt gttgttattt tgcacggttt ttgtcgggta tggatttgat gattgctgcg
    30421 caaatgcacg ttttcagggc aataattgcc tgatttttgg gcaagataat taacgttttc
    30481 gtatagtaac agagtgaaag tattttaaat caaagaatta gatttgtgct ttaatcgcaa
    30541 attgcgatct aaatcaaatt aatcggttaa agataaccgc agcggggccg acataaactc
    30601 tgacaagaag ttaacaacca tataacctgc acaggacgcg aacatgtctt ctcatccgta
    30661 tgtcacccag caaaataccc cgctggcgga cgataccact ctgatgtcca ctaccgatct
    30721 gcaaagctat atcactcatg ctaatgacac ttttgtgcag gtgagcggct ttaccttgca
    30781 agagttacaa gggcagccgc acaacatggt gcgtcaccca gatatgccaa aagcggcgtt
    30841 tgcggatatg tggttcaccc tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg
    30901 ccgcaaaaat ggcgaccatt attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg
    30961 aaaaatcagt ggctatatgt cgattcgtac ccgggcgacg gatgaagaga ttgcggcggt
    31021 ggagccgctg tacaaagcgt tgaactccgg acgtaccagt aagcgtattc ataaaggcct
    31081 ggtggtgcgt aaaggctggc tgggtaaact gccttcatta ccgcttcgct ggcggacgcg
    31141 tggagtgatg accctgatgt ttatcttgct ggcggccatg ctttggtttg ttgctgcccc
    31201 ggtggtgacg tatttcctct gtgtgttagt ggtattgttg gcaagcgctt gttttgaatg
    31261 gcagattgtg cgcccgatag aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga
    31321 gcgtaatagt gttgagcacc tgaatcgcag cgatgagctg gggctgacat tacgcgcggt
    31381 agggcagctt ggcctgatgt gccgttggct aattaacgat gtctcaagcc aggtgtccag
    31441 tgtcagaaat ggcagtgaga cgctggcgaa aggcaccgat gaactgaacg aacataccca
    31501 gcagacagtt gataacgttc agcaaacggt ggcgaccatg aaccaaatgg cggcgtcggt
    31561 gaaacagaac tctgccacgg cgtcggctgc cgataaactt tctatcaccg ccagtaatgc
    31621 ggcagtgcag ggtggggagg cgatgaccac ggtgatcaag acaatggacg atatcgccga
    31681 cagtacccag cgcattggca ccattacttc gctgattaac gatattgcgt ttcagaccaa
    31741 tattctggcc ctgaatgcgg cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt
    31801 tgcagtggtg gcaggggaag tgcgtcattt agccagccgc agcgctaatg ctgccaacga
    31861 tattcgcaag ctgattgatg ccagtgctga taaggtgcaa ttcggttcgc agcaggtaca
    31921 cgccgccgga cggacgatgg aagatattgt ggcacaggtg aaaaacgtca cccagttgat
    31981 cgcccagatt agccattcaa cgctggaaca ggccgatggg ctttccagcc tgacccgtgc
    32041 agtggatgag cttaacctga tcacccagaa aaatgccgag ctggtggaag agagtgcgca
    32101 ggtgtcggcg atggtgaaac accgcgccag ccgactggaa gacgcggtga cggtgctgca
    32161 ttaatcggta tgccggatcc ggcggttgga gcacaatgcc tgatgcggcg attaatccag
    32221 ttgagtaata tcaaaagcgg cgcggtcgat caccgcaatg atctttttta tttgtgcatc
    32281 actgatatcg ctctggttga cgcgtaaatc cagcaccgct ttaaaattat ccagcgcccg
    32341 cttcatttgc gggttctggc gcagcgccgc gccaacgcaa cgcgctttga tgcgttcttc
    32401 aatcatctcc acctgttcgc ggttttcttc cagccactgc gcgccttgtt cggtcagcgc
    32461 aatctgcttc ttaccacctt cctcttcgcg gatggtaatc agcgactgct cctgcagaaa
    32521 atccagcgtc gggtagatga cgcccgggct tggggtgtaa ttcccctggg ttagattctc
    32581 aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc gagagaatat ccagaatcac
    32641 cagacgcaat tcaccgtgac caaagaaacg ttgccgacga ccgccgccgc gaccgtggcg
    32701 accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt ccgcagtgct cgtgttctga
    32761 cttctcacct ttgcagcagc cctcatggcg tggctggcct tcatgtttac aacacccttc
    32821 gtgatgatgg ctcatatcag cctccttttg cttgattaga tatatctaat ttatatctga
    32881 tgtgagcatt actgcaagta caaaaatgat ttttaaacgt ggaagcacct cacatctagg
    32941 ccggataaga cgttcacgcc gcatccggca gcattgctat ggcactgaat gccttcacat
    33001 acttcagaca tatcagttct acaaatcgct tgcatttatc atgattaaca atcattatca
    33061 tttgcgaatt ttatttagat atatctgatt accatcacga aggcgataac aatgaataac
    33121 accccccgct acccgcagcg cgttcgcaat gatctgcgct tccgtgaact gactgtgcta
    33181 cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc ttggcggcga ggcgttggac
    33241 ggttttacat cgcgcggctt tgacgatcac agcaaactct tctttcctca acctgacgct
    33301 cactttgtgc cgccaacggt aacggaagag ggcatcgtct ggccggaagg accacgccca
    33361 ccgtcgcgtg actatacgcc actgtatgac gaactacgcc atgaactggc gatcgatttc
    33421 tttattcacg acggtggggt cgccagcggc tgggcgatgc aggcgcaacc gggcgataaa
    33481 cttacggtgg caggtccgcg cggttcgctg gtggtgccgg aagattacgc gtatcagctg
    33541 tatgtctgcg atgaatccgg aatgcccgca ctgcgccgcc gcctggaaac gttgagcaaa
    33601 cttgccgtta aaccgcaagt tagtgcgctg gttagcgtgc gggataacgc ctgtcaggat
    33661 tatctcgcgc accttgatgg ttttaatatc gaatggctgg cacatgatga gcaggcggta
    33721 gatgcgcgtc tggcgcagat gcaaatccct gccgatgatt acttcatctg gataaccggc
    33781 gaaggtaaag tcgttaagaa tttaagccgc cgctttgaag cggaaaagta tgacccacag
    33841 cgggtacgtg cagcggctta ctggcacgca aagtaactga tatataaaaa gaaaaaggct
    33901 gacgatttct cgtcagcctt tgctatatct ggtggcccct gctggacttg aaccagcgac
    33961 caagcgatta tgagtcgcct gctctaacca ctgagctaag gggccatggt agcggattat
    34021 aaagtaactc cgtgtcgcaa tccagccatt accgcgcgcc tgctgttttt ataaacaatg
    34081 tattttcaat ccgttatact tttcctggtg atgttagaaa ggagtaaata tggttgagga
    34141 tattttggct ccagggttac gggtcgtgtt ttgcggtatc aaccctgggc tttcatccgc
    34201 cgggactggt tttccctttg ctcatccggc aaatcgcttc tggaaggtga tatatcaggc
    34261 cgggtttacc gaccgtcagt tgaagccgca ggaggcacag catctgctgg attatcgttg
    34321 tggcgtcacc aaactggtag accgtccaac ggtgcaagcc aatgaagttt caaagcagga
    34381 gctgcacgca ggtgggcgta agctgattga aaaaattgaa gattatcagc cgcaggcgtt
    34441 ggcgattctg ggcaaacaag catatgaaca gggattcagc cagcgcggtg cacagtgggg
    34501 gaaacaaacg ctcaccattg gttcgacgca gatttgggtg ctgccaaatc ccagcggttt
    34561 aagtcgcgtt tcactggaga aactggttga agcgtatcgc gagctggacc aggcgctggt
    34621 agtgcgtggg cgataaaaaa cgccaccgaa a
//
LOCUS       DADKXM010000026        34261 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.026, whole genome shotgun sequence.
ACCESSION   DADKXM010000026 DADKXM010000000
VERSION     DADKXM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.026"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..332)
                     /locus_tag="JQR05_001278"
     CDS             complement(<1..332)
                     /locus_tag="JQR05_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323482.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYLQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="JQR05_001279"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="JQR05_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY5323483.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="JQR05_001280"
     CDS             complement(1693..2073)
                     /locus_tag="JQR05_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY5323484.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="JQR05_001281"
     CDS             complement(2087..2503)
                     /locus_tag="JQR05_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323485.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="JQR05_001282"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="JQR05_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY5323486.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="JQR05_001283"
     CDS             complement(3504..3971)
                     /locus_tag="JQR05_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY5323487.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASVLDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="JQR05_001284"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="JQR05_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY5323488.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="JQR05_001285"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="JQR05_001285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY5323489.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="JQR05_001286"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="JQR05_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY5323490.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAGSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="JQR05_001287"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="JQR05_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY5323491.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="JQR05_001288"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="JQR05_001288"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY5323492.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="JQR05_001289"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="JQR05_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY5323493.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="JQR05_001290"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="JQR05_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY5323494.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPSSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="JQR05_001291"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="JQR05_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY5323495.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSSERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="JQR05_001292"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="JQR05_001292"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY5323496.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="JQR05_001293"
     CDS             complement(14626..15441)
                     /locus_tag="JQR05_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY5323497.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="JQR05_001294"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="JQR05_001294"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY5323498.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="JQR05_001295"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="JQR05_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY5323499.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="JQR05_001296"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="JQR05_001296"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY5323500.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="JQR05_001297"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="JQR05_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY5323501.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="JQR05_001298"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="JQR05_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY5323502.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="JQR05_001299"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="JQR05_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY5323503.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="JQR05_001300"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="JQR05_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY5323504.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSAEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="JQR05_001301"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="JQR05_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY5323505.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="JQR05_001302"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="JQR05_001302"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY5323506.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="JQR05_001303"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="JQR05_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY5323507.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="JQR05_001304"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="JQR05_001304"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY5323508.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="JQR05_001305"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="JQR05_001305"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY5323509.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="JQR05_001306"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="JQR05_001306"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY5323510.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWSCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGMGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="JQR05_001307"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="JQR05_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY5323511.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNDLGFWRYKEDSKGKPKKEEDAAVEDPLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="JQR05_001308"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="JQR05_001308"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY5323512.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="JQR05_001309"
     CDS             31574..32188
                     /locus_tag="JQR05_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY5323513.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="JQR05_001310"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="JQR05_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY5323514.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="JQR05_001311"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="JQR05_001311"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY5323515.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8044 a         8660 c         9269 g         8288 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc aagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctggatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcttggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggaggctta gcctggctaa
     1681 gcctcctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggttgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaatgaaaa tatatatcgg cgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag caccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagctgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagagg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgt cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct ggcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtggg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgccag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg cgtacccgtg
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagcata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgagctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgaga aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atccttcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgggccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagatga
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gcttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agctcattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgttggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 gcggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacctgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgccagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac tgatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggt gccggatcga caatgtcttt cggtttatat accgatagct gatgaatcac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gttgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cttcgcctgt ctccgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agaagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacat tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccctgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccata ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ccatatgctc cacgccgcca accagacatg cctgcgcatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaactc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag cgggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggtcgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttccagc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgta ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tactaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgttgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctact tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 c
//
LOCUS       DADKXM010000027        33912 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.027, whole genome shotgun sequence.
ACCESSION   DADKXM010000027 DADKXM010000000
VERSION     DADKXM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.027"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(40..891)
                     /gene="pdxK"
                     /locus_tag="JQR05_001312"
                     /gene_synonym="thiJ"
     CDS             complement(40..891)
                     /gene="pdxK"
                     /locus_tag="JQR05_001312"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY5323516.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHEIDELI
                     LPPLAEA"
     gene            996..1370
                     /locus_tag="JQR05_001313"
     CDS             996..1370
                     /locus_tag="JQR05_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY5323517.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQMDKTVPAEGN"
     gene            1403..2126
                     /locus_tag="JQR05_001314"
                     /pseudo
     CDS             1403..2126
                     /locus_tag="JQR05_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(2330..3241)
                     /gene="cysM"
                     /locus_tag="JQR05_001315"
     CDS             complement(2330..3241)
                     /gene="cysM"
                     /locus_tag="JQR05_001315"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY5323518.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMLELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(3375..4472)
                     /gene="cysA"
                     /locus_tag="JQR05_001316"
     CDS             complement(3375..4472)
                     /gene="cysA"
                     /locus_tag="JQR05_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY5323519.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(4462..5337)
                     /gene="cysW"
                     /locus_tag="JQR05_001317"
     CDS             complement(4462..5337)
                     /gene="cysW"
                     /locus_tag="JQR05_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY5323520.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(5337..6170)
                     /gene="cysT"
                     /locus_tag="JQR05_001318"
     CDS             complement(5337..6170)
                     /gene="cysT"
                     /locus_tag="JQR05_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY5323521.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYIWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGLEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(6170..7186)
                     /locus_tag="JQR05_001319"
     CDS             complement(6170..7186)
                     /locus_tag="JQR05_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5323522.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(7490..8281)
                     /gene="ucpA"
                     /locus_tag="JQR05_001320"
     CDS             complement(7490..8281)
                     /gene="ucpA"
                     /locus_tag="JQR05_001320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY5323523.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(8410..9267)
                     /gene="murR"
                     /locus_tag="JQR05_001321"
     CDS             complement(8410..9267)
                     /gene="murR"
                     /locus_tag="JQR05_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY5323524.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            9431..10327
                     /gene="murQ"
                     /locus_tag="JQR05_001322"
     CDS             9431..10327
                     /gene="murQ"
                     /locus_tag="JQR05_001322"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY5323525.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTELMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            10331..11755
                     /gene="murP"
                     /locus_tag="JQR05_001323"
     CDS             10331..11755
                     /gene="murP"
                     /locus_tag="JQR05_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY5323526.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(11935..12834)
                     /gene="yfeX"
                     /locus_tag="JQR05_001324"
     CDS             complement(11935..12834)
                     /gene="yfeX"
                     /locus_tag="JQR05_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY5323527.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12930..13505)
                     /locus_tag="JQR05_001325"
     CDS             complement(12930..13505)
                     /locus_tag="JQR05_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY5323528.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13566..14015)
                     /locus_tag="JQR05_001326"
     CDS             complement(13566..14015)
                     /locus_tag="JQR05_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY5323529.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14002..14427)
                     /locus_tag="JQR05_001327"
     CDS             complement(14002..14427)
                     /locus_tag="JQR05_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY5323530.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14641..15510
                     /gene="amiA"
                     /locus_tag="JQR05_001328"
     CDS             14641..15510
                     /gene="amiA"
                     /locus_tag="JQR05_001328"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY5323531.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVINYF
                     HWFDNQKAHSKKR"
     gene            15514..16413
                     /gene="hemF"
                     /locus_tag="JQR05_001329"
     CDS             15514..16413
                     /gene="hemF"
                     /locus_tag="JQR05_001329"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY5323532.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16419..17471)
                     /gene="eutR"
                     /locus_tag="JQR05_001330"
     CDS             complement(16419..17471)
                     /gene="eutR"
                     /locus_tag="JQR05_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY5323533.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVYQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17517..18017)
                     /gene="eutK"
                     /locus_tag="JQR05_001331"
     CDS             complement(17517..18017)
                     /gene="eutK"
                     /locus_tag="JQR05_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY5323534.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLTACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18030..18689)
                     /gene="eutL"
                     /locus_tag="JQR05_001332"
     CDS             complement(18030..18689)
                     /gene="eutL"
                     /locus_tag="JQR05_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY5323535.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPQHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTSGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18699..19586)
                     /locus_tag="JQR05_001333"
     CDS             complement(18699..19586)
                     /locus_tag="JQR05_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY5323536.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19607..20968)
                     /locus_tag="JQR05_001334"
     CDS             complement(19607..20968)
                     /locus_tag="JQR05_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY5323537.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            21156..22364
                     /locus_tag="JQR05_001335"
     CDS             21156..22364
                     /locus_tag="JQR05_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5323538.1"
                     /translation="MSKTPLTAKAIEAAQPQDKPYKLTDSMTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNAI
                     ASFKAVAEDWMATKVSGWSESYTKQVRSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVNRNPVADLDTALKARRPGHNAWIPINEIPA
                     FYKALERAGSVQIQTAILLLILTALRTAELRLMRWEWVDLQSATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSAFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYSPDWIEIQLAHVPKGVRGVYNQAAYLKQRRAMMQDYSDCIDSIL
                     AGDGNPLEPE"
     gene            22610..23197
                     /locus_tag="JQR05_001336"
     CDS             22610..23197
                     /locus_tag="JQR05_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323539.1"
                     /translation="MNEIKKPGKAATSSGRYEKQAKGEFLMSDMLAVEQETNNDVRQY
                     LNKINELRNKAPKNEETKHEEHTPDHYEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
                     ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRL
                     VHHFKLVERTEGYIPGSAERECNEYQLLFKPYNMK"
     gene            24670..24861
                     /locus_tag="JQR05_001337"
     CDS             24670..24861
                     /locus_tag="JQR05_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5323540.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIDSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            25001..25264
                     /locus_tag="JQR05_001338"
     CDS             25001..25264
                     /locus_tag="JQR05_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323541.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            25282..25824
                     /locus_tag="JQR05_001339"
     CDS             25282..25824
                     /locus_tag="JQR05_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323542.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANKGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNVEKMEMHKQSGAPTPDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            25921..26250
                     /locus_tag="JQR05_001340"
     CDS             25921..26250
                     /locus_tag="JQR05_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323543.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            26289..26495
                     /locus_tag="JQR05_001341"
     CDS             26289..26495
                     /locus_tag="JQR05_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323544.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            26954..27292
                     /locus_tag="JQR05_001342"
     CDS             26954..27292
                     /locus_tag="JQR05_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5323545.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            27273..27551
                     /locus_tag="JQR05_001343"
     CDS             27273..27551
                     /locus_tag="JQR05_001343"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323546.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDEKTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            27544..29004
                     /locus_tag="JQR05_001344"
     CDS             27544..29004
                     /locus_tag="JQR05_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5323547.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            29026..29847
                     /gene="murI"
                     /locus_tag="JQR05_001345"
     CDS             29026..29847
                     /gene="murI"
                     /locus_tag="JQR05_001345"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY5323548.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(29966..30660)
                     /locus_tag="JQR05_001346"
     CDS             complement(join(29966..30414,30414..30660))
                     /locus_tag="JQR05_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5323549.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(31175..32587)
                     /gene="eutA"
                     /locus_tag="JQR05_001347"
     CDS             complement(31175..32587)
                     /gene="eutA"
                     /locus_tag="JQR05_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY5323550.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGVAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPSRIR"
     gene            complement(32584..33810)
                     /gene="eutH"
                     /locus_tag="JQR05_001348"
     CDS             complement(32584..33810)
                     /gene="eutH"
                     /locus_tag="JQR05_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAY5323551.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMR
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
BASE COUNT         8582 a         8847 c         8306 g         8177 t
ORIGIN      
        1 tccttaactg atcggcatta cgcccatcgg cgccattttt tatgcttccg ccagcggcgg
       61 caaaatcaat tcatcgatct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa
      121 ccccgcacgg tgcaccgcat cgtttaacgc cttccctttc agcaaaccac tgataagctg
      181 agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat gggaaatgac
      241 attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt cattaccgga
      301 ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg cggcagcaat
      361 ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa agatattggg
      421 ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa ggtcaggttt
      481 gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca ataggtcagg
      541 atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc
      601 catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct gaagcgcacg
      661 caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt cataatgcgg
      721 cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct gttttatagc
      781 aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca cggcgacgat
      841 atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca tttaattttt
      901 tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt ctggcagtca
      961 aatcggctat tacaaaacaa ggataaggta attcagtgaa gaaaatcatt tgtctggcca
     1021 ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa gaagcccgca
     1081 tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg cgcaacggtg
     1141 atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc aatacccgta
     1201 accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca tcgattaccg
     1261 ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag ccttttttac
     1321 atgcgttaat tgcgcagatg gataaaacgg tgcctgcgga aggaaattaa tccgctttgg
     1381 taaggcattt acaggaggta acatgaaaaa atgctttatt tgtcacgatg aaaaatcgaa
     1441 taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg gcaaggtagg
     1501 tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc tgaaagaagc
     1561 cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag actttaactt
     1621 catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa cgtcacaccc
     1681 aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg aagaatctcc
     1741 ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc tccgttctgg
     1801 actgtgctga tttccctcaa atgttaattg aaactatgtg gggtatgaaa tacatcgcta
     1861 tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa atgagctcta
     1921 tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc atgggtaaaa
     1981 tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag cttaccgcaa
     2041 aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg attgatgacc
     2101 ttactgcttt tccacacgaa aattaaaaac tgcatttgtc ggcagcaaca actgccgaca
     2161 aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa cgcctgatcc
     2221 ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg ataagcggag
     2281 cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct taaatccctg
     2341 ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga tcgccacgat
     2401 cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc agtgctccag
     2461 caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc agttcgagca
     2521 tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc gaagcgttga
     2581 aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg ctgccttctt
     2641 ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag cgtgaaacgc
     2701 cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc ccgccggttt
     2761 gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg ggattattga
     2821 actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga tcgcgcgcac
     2881 cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca cgcatcgccg
     2941 cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag cctttcagcg
     3001 cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt aagacatcac
     3061 ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc gcacgatctt
     3121 tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg ccgttatccg
     3181 gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct aatgtactca
     3241 cgatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta tcaggcctac
     3301 aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg ggcgtttaac
     3361 attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata
     3421 cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg ctcgccacgc
     3481 tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca ccccagcggc
     3541 tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg taccggcagc
     3601 ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag gaagagatcc
     3661 accggaccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc aacatggaac
     3721 tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc gagtacaaaa
     3781 cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat attcccctga
     3841 ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg ggtcacaaac
     3901 acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag ctctttacgc
     3961 acctgcgcat ccagcgcgcc aaacggttca tcaagcagca gaatttgcgg ttctacagcc
     4021 agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc cggataacga
     4081 tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc tttgatggct
     4141 gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat attgtcgaat
     4201 accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt acgatcgcgc
     4261 gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt ttgatgctcc
     4321 agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc cagcaacgcg
     4381 accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt gcgaccaaaa
     4441 gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg cgcgtttttc
     4501 ctgattctcc aagcgccact gcaacatact ctttaaaaac agggtgataa tcgccatcag
     4561 cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct ccaacaattc
     4621 aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca ccgacactgc
     4681 accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca gcgcccagcg
     4741 aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa gcaaaatcgc
     4801 cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc gcaccacaaa
     4861 cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca taatttgcag
     4921 attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca gcagatacac
     4981 cagaccggca accaccggcg atacggcaaa cggaatgtcc agtagcgtca gcagtaactg
     5041 gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga acaccaggtt
     5101 taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca gcatgtccgg
     5161 atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca cgaagatgta
     5221 aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa tcagaaacca
     5281 tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt ccgccattaa
     5341 tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa cagcagcagc
     5401 agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc aaactcctgt
     5461 aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt tccggcgata
     5521 aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac gcccgccacc
     5581 agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg cgttgcacca
     5641 agcgtttccg ccgcttcttc atattccagg cctaactctt ccagcaccgg ctgcacggta
     5701 cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag ccagatatag
     5761 gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac ggaaaagagc
     5821 gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc catcagcgca
     5881 tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat cagcagaccg
     5941 aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt gtaggccgcg
     6001 actacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc agaaagctgc
     6061 atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag actggtgcca
     6121 aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat tacttacgcc
     6181 ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc atcacttccg
     6241 gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat ttatctttca
     6301 gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg atggtctgcg
     6361 cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta ccgttggcct
     6421 gcacattttt atcgacccac gccaccggga attccgccag aatattggtt ttcgggatca
     6481 ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc gattcaaagc
     6541 taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca cgaccgccgg
     6601 tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt ttggctttat
     6661 cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa cgcgcgttac
     6721 ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg tcgttccagt
     6781 cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag tagaacggcg
     6841 agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct ttgtcgtgca
     6901 ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag ccctgcaaaa
     6961 tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt ttgtcgccgc
     7021 cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac agctcgcggg
     7081 agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc agcagcagag
     7141 aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg cacccttata
     7201 aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga gtaacggttt
     7261 tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt caacaggcgt
     7321 aattgttgca ggcagtttgg acacggacag cgcggagaaa ccggagcgta cacatagtac
     7381 atgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct ggtgaactta
     7441 gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat cagataccga
     7501 cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta ccggttaaat
     7561 agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc ggatcggcga
     7621 ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga tcttccgggt
     7681 tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg cagatggcgt
     7741 taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg ccaacaatcg
     7801 ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca ccagtgactg
     7861 aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc accgccttcg
     7921 tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct tcatcgctca
     7981 tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc
     8041 cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca cgcacatcgg
     8101 cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc ttttcgatct
     8161 caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt ctggcaattc
     8221 cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca
     8281 ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga aaatacagga
     8341 taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat ccagtcgctg
     8401 ctctatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca ttttcaatga
     8461 cgccacatca ttgagttgca caagaccaac aaacagtaaa tcggtaacag aattttgcgc
     8521 cgtgcgggta gacatcgacg agctacgcca ttcggtttca ccggaaacgg tatcgagcgt
     8581 gaaatgcgcc agccgccgca gcggagagtc agccagagaa gtaatggcaa tcaccgttgc
     8641 cccctgcttt cgtgccgctt ctgcgcacaa gacaatctct tttttactgc cgctgtaaga
     8701 gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt
     8761 gtcggcttcg caggccacgc gatagccaat tttcatcagt ttgaacgaca gatcgcgccc
     8821 caccagcgcc gatccgccca ggcccgttat ctggataaac ggtgctttac tgatgacctc
     8881 aatgattttt tgcagccgcg cgtagtcgaa caacgcacag gtttgctcca gcgccagctc
     8941 tttttcgcga ttcagtttgc gggcaatcac ttccagcgaa tcatcactgg taatcgaact
     9001 gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg gcactgtatt cgccaattaa
     9061 cgccatacgc aattcagtaa atccttgcgc gccgagcttc tgggcaaatt tcacaatgct
     9121 cgactggctg ataccgagct gtttcgccat ctgccgtgaa gagacggatt tcagctcgct
     9181 cactctggcc cgcagaaagt cagcaatttt ttgctcgttc ccggtaaatt cactttccgc
     9241 attgcgtatt ttcgtcaggt acaacatctt ccactctcct gggctggcag caaaaattcc
     9301 gcttaagttc ttatgacgct cttcacactc tgcgagtgta gactcaatga ttcctttaag
     9361 tctgtcaatt cgggatatat gattccatac atctgcaatt atttgatcgt aaatagtaag
     9421 gtcaccaccg atgcaactag aaaagatgat tactgaaggc tcgaacaccg cctcggctga
     9481 aattgaccgc gtatcgacgc tggaaatgtg ccggattatc aacgatgaag ataaaaccgt
     9541 accgcttgcc gttgagcgcg tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc
     9601 ccaggtcagc ggcggcggtc gtctgattta tctcggtgcg ggaacatccg gtcgtctggg
     9661 gattctggat gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg
     9721 tttgattgct ggaggcgaat atgccattca gcacgcggtg gaaggcgctg aagatagccg
     9781 ggaaggtggt gttaacgatc tgaaaaatat taatttaacg gcacaggatg tggtggttgg
     9841 cattgctgcc agcggtcgca cgccgtatgt gattgccgga ctggaatacg cacgccagct
     9901 cggctgccgc acagtgggaa tttcctgtaa tccggggagc gccgtttcaa ccaccgctga
     9961 gtttgccatt acaccgattg taggtgccga agttgttacc ggctcttcgc ggatgaaagc
    10021 aggtacagcg cagaaactgg tgctcaatat gctttccacc gagctgatga ttaaatccgg
    10081 caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg
    10141 ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt
    10201 aattgcctgc gaacgcaact gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc
    10261 cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg ttttagacaa
    10321 ggaataaccc atggccaaag agatcagcag tgaacttctg aacaccattc ttacccgtgt
    10381 cggcggaccg ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt
    10441 acatgacagt tcactggttg atcccaatat caaaacgctg gaaggcgtga agggcgtcat
    10501 tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa
    10561 agcgatgagc gagctgctgg gcgaggcacc ggtacaggat gccgcagaaa tcgccgccca
    10621 gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt
    10681 cgccaccatc ttcacgccgc tgatccccgg ttttattgcc gccggtctgc tgctggggat
    10741 agcgacgtta attgccacgg tgatgcacgt tccggcagat gctcagggca cgctacccga
    10801 cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc accttcttgg tgattctggt
    10861 gggctataac gccgcccagg catttggcgg cacgggcgtc aatggcgcaa ttattgccgc
    10921 actctttttg ctcggttata accccgctgc aaccaccggt tactacgccg gttttcacga
    10981 tttctttggt ctgcccatcg atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg
    11041 ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg ccggacgatc tcgacatgct
    11101 gctgacctcg ttaatcaccc tgctgatcac cgctacgctt gcgtacctga tcattatgcc
    11161 gctgggtggc tggttattcg aaggtatgtc atggctgttt atgcacctga acagtaatcc
    11221 gttcggttgt gcggttttag ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca
    11281 gggctttatt cctgtttacc tcgcgttaat ggacagccag ggattcaaca gcttatttcc
    11341 catcctttca atggcaggcg cgggccaggt gggcgcggcg ctggcactct actggcgggc
    11401 gcaaccgcac agtgcgctac gcagtcaggt acgcggggcg attattcccg gcctgctggg
    11461 cgttggtgaa ccgctgattt acggtgtcac cctgccccgc atgaagccgt ttgttaccgc
    11521 ctgtttaggc ggcgcggcgg gcggtttgtt tatcggattg atagcctggt ggggtctgcc
    11581 gatgggctta aacagcgcct ttgggccgtc tggtctggtg gcgctgccac tgatgacttc
    11641 cgcacaaggc atccttccgg caatggcggt ttatgctggc gggattctgg tggcatgggt
    11701 ttgcgggttt attttcacca cgctctttgg ctgccgtaac gtcaatctgg actgatatta
    11761 tgaaacggac aatgctctat ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa
    11821 aataccctgt tctgacagaa ggtagttgat caagtatatg cggcgttaaa gcagtaataa
    11881 aaaggccagt cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag
    11941 cgccatcaac ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa
    12001 acgcaacatc gcatcacgtt tgccatcggt atcgccaaac atgctcagca gttgctgctc
    12061 aatgttatgc agacgcgcgc agtaggcgca gaagtacaga ccgtgagtgc cactggcggt
    12121 gccgtacggc aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac
    12181 gcgggtgagg tgagaggttt ccggacgttc gtcgccgtca atctcttcgt tggcctcttt
    12241 ggtgcgcccg atcatcatct cctgatcgtg aacgctcatc cggttgagct gcttcaggtt
    12301 gtgctcccaa cgctgcacaa acacatagct gccgccggca tccacgccgt ctttgataac
    12361 cgccacttcg cggcgcgtct cttcacccgc cgggttttct gtaccgtcaa caaagccgct
    12421 caggtcacgc tcttccaccc aacggaagcc gtggatctct tctttcactt caatgcagtc
    12481 accaaaggct tccatcgccg cctgagcgac agagaagttt acgtcgtgac gcagagaaag
    12541 aatgtggatc aacacatcga actgggtcgt cggcgcaagg cctttaccgt agcccggaaa
    12601 atctttcagc tcttctgccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa
    12661 ggcaaccacc gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc
    12721 ggcaaatgtt ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat
    12781 ccaaatcgcc gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc
    12841 tcctgaaaat aagaatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt
    12901 ttcgcgacgg cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc
    12961 cagtttttca gggtatcgtc cgaaggcatt aacccttcag gaccacgcca ttccccgctg
    13021 aactggtagc taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca
    13081 ccatcagctt tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg
    13141 attttaacac cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg
    13201 ccttgatcgc cgttaatcac catcgccacg ttgtcgcctt tcatcacttc aaaaaagcgc
    13261 accacgttgc cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca
    13321 tcagcaatgg cttgttcttg cagtggtgtg gacgccgtta attcacccac gccctgctcg
    13381 ctcactttgg tggacgatcc aaaccagttc cacgggttag cggcagacca gttaactgac
    13441 gacatcgtgg aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcagcgat
    13501 ttcatgtcac ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag
    13561 tgccgttatt ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag
    13621 attaacgcca caatatccgc cactaccagc gccagaccga taccgctaac ggattcaccg
    13681 ttcagccaca gccacggttg ccagcaaagt aaaaccacct gcgccaacag caacagaaaa
    13741 tagagcacat gccaggtgcg aggaaacgaa gcccgccgac cgctcaacaa aaacgccagc
    13801 accgccggaa taccgggaat cagccccagc cagaaattat cgtgatcggg ataaaacaga
    13861 tttagcagcg cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt
    13921 cgcgcctgaa gcaatagcac aagccagaag agcaagggta aacgcaggcg tccgtgcgca
    13981 tcataatgga caggatggaa ctcagtactc ttcatcttca atcaaacgct tacccagact
    14041 cagcacgtcg gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc
    14101 ttccggcacg ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccagccg
    14161 gttaagcaac gcattggcaa ttccacgccc acgaaactct ggatgcacgc caagataata
    14221 agcagacccg cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgtttac
    14281 ctcagcgacc aaaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg
    14341 atcgttccac ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc
    14401 ttcctggcga aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct
    14461 gattatggcg tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc
    14521 ataatgggga gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaaccgt
    14581 cgtaacggat tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt
    14641 atgagcactt ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt
    14701 ttagctgccc tgacgttgtc aggaatgtcg caagccatcg ccaaagacga acctttaaaa
    14761 accagcaacg gacacagcaa gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt
    14821 ctcgatccag gccacggcgg aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa
    14881 aaacatgtgg tgctggcgat tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt
    14941 gatgcgcgtt taacgcgttc tggcgatacg tttatcccac tttacgatcg cgttgaaatc
    15001 gcccataaac atggcgcaga tctgtttatg tcaattcatg ccgatggctt taccaacccg
    15061 aaagctgccg gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg
    15121 aaatacctgt ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac
    15181 aaggatcacc tattgcagca agtactgttt gatctggtgc aaaccgatac tattaaaaac
    15241 agcctgacgc ttggctcgca tattctgaaa aagattaagc cggtgcataa actgcacagc
    15301 cgcaacaccg aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg
    15361 gtggaaacct cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcgttt
    15421 cgtcagaaaa tcgccacagc gattgctgaa ggcgtgatca attatttcca ctggttcgac
    15481 aaccagaaag cacattcgaa aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa
    15541 cagtttctgc tcaaccttca ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca
    15601 gaatttgtcg aagatagttg gcagcgcgaa gctggcggcg gcgggcgcag tcgggtgttg
    15661 cgtaatggtg gtgttttcga acaggcgggc gtcaactttt ctcacgtcca cggtgaggcg
    15721 atgcctgctt ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg
    15781 ggcgtttcac tggtagtgca tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg
    15841 cgttttttta ttgccgaaaa accgggtgca gagcccgttt ggtggtttgg cggcggcttc
    15901 gatttaaccc ctttctatgg ttttgaagaa gacgccattc actggcaccg caccgcccgt
    15961 gacctgtgcc tgccatttgg tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat
    16021 tacttctacc tcaaacatcg caacgaacag cgcggcattg gcgggctgtt ctttgatgat
    16081 ctgaacacgc cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac
    16141 accgacgctt atttaccaat tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag
    16201 cgcaattttc agctctaccg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc
    16261 ggcacgctgt ttggcctgca aactggcggg cgcaccgagt cgatcctgat gtcaatgccg
    16321 ccactggtac gctgggagta tgattatcag ccaaaagatg gcagcccaga agcggcgtta
    16381 agtgagttta ttaaggtcag ggattgggtg taactccctc acccccattc ccgcatccgc
    16441 tgatgcaacg tcaacgacgg cttctcggca aacagctgct ggtaatccgt ggcaaattgc
    16501 cccagatgcc agaatcccca ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac
    16561 gggcttatca gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca cgcgtttggg
    16621 ccaatgccta aaatagcgtg aaacgcgttt tgtagcgtgc ggcgactgac atgcagttga
    16681 ttacacaaat ccagcaccgt caccggttcg gacatgtttt ccagcacata ttcacgggcg
    16741 cgggaaagca accgacggta actctgatga ctgatgcttt ccgccgtcac cattggttgc
    16801 gcttcttcca gcatcgcccc catcgccatt agcaaattat cccccagcac ttttcgcact
    16861 gcaggctgat ggagattttc cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag
    16921 ccccacagcg cggctttatg ctgctctttc acttccagcg ccgactggct acgcagcata
    16981 tgtaataccc gatccggatt gtgcaaaaag ttagcctgac gggtgatgac atcttcagac
    17041 agcactacgc ccaggatcgt ataatcgtcc ggcgtactta attcaaactc ggttccaccg
    17101 gggcgcgtcg ctatttctgc gcttcccaga cattgcgaac cgataaatcc ctgctcaccg
    17161 cgcgtcgccg gaatgccaaa ccagaacgag ttcggccaga ccaggcacga ctgacgcagc
    17221 gccagaccgg tgtattcacg aaaaacctga atatcatcga gtagaatttc cgtaaattca
    17281 ccatgaaact tgcccggatg cagctgatcg taaatctgct gccaggcggt aatcgtcaaa
    17341 gcatgttcat agacatccgt tgtctgtcgt tgataaacat tatccacttc gaccttcggc
    17401 gtgagcttca ggttttcggg taagggttca tgataaagat ggtgcaaatt ggctgtacgg
    17461 gtctttttca tgatgttaat gccgggtgtt gtaggacacc cggcacctcc gacaggttaa
    17521 tggggcttga gacgataacg actactgcgt ttacgtaacg tcccggcaga aaagagctgt
    17581 tcgagcgcat ttctggcttt ttccagcggc cagccaaagt gggcggccac ttctcctgcc
    17641 gtcattccct gacatactga tgttaacagc gccaacaact cgtcagcaga ttccgcgacg
    17701 ataactggcg cgtcgggttc ctttacgggt tgcttcggct ggcggttaaa gccagtaacc
    17761 agccactggg tgtcatcgtc tggccgaccg atctccttac ggctgatgac acgcccggta
    17821 cgcatcgcgg cagcacaacc tgcgtccagc gccgcacgac acgccgtcag atcgccttcc
    17881 accaccagcg ttaagcgacc agggtcaagc acttcgtgac tgagcagacg aacgttagct
    17941 gctttgagca tggcatccgc tgcatctatc gcggcgacca tgccgtccac ttccagcaat
    18001 cccagtgcat tgatcatcgg caacctccgt tacgcacgct ggattggatt acgcgcgatt
    18061 tccagcactg catcggtaaa ggcgttacag gctgctttac acgcggcctg gctaccggtt
    18121 aaaaatgctg ccgagtagtt ggtttcagac ggtggcggga cgtaggtcac cagctgtacg
    18181 tcggcagatt tcaacgcggc atcaatgccg taggtcgctt ccagcggcgg tgccaccaga
    18241 tacgccatcg gatcgccaag cgtgatcccg gaggttgatg aaagataaga accggtacgc
    18301 gaaaccacat gcgccaggaa ggcggtgttt tctgcgtcat tggcccactg gaaagctgcg
    18361 ccattttcaa tatgtgcaac catcgcatcc agaccggcac gcacttccgc cgggtttggc
    18421 ccaccaagca taatcagcac ttcaccggca gtcggtgacg ggccgtgtgc cgctcccgca
    18481 tacagcgagc gaccatatac cacttcgacc atcgcttgtt tggtcgcttc gtcagccgca
    18541 atgtaggtta cgtcgtcaga atcggcagaa atcagcccga ggctacgaat atgctgcggc
    18601 aattttagtt cacgcgcaaa ttcagcgtta acggaggcaa tcacccgcat ggcggttacc
    18661 gacggtcgaa tcaaatctaa agctggcatg atgcctcctt atcgggtcat gttgatgccg
    18721 gatgctttct gctccagcat acgtttcgcc aaatccacaa tgacggctgc ggcttcaacc
    18781 ggcggcgtgc cgccctggtg aatgttagag atacaggtgc gatcggcctc aacggtggtc
    18841 gccatacgcg gcgagtaaac ggcgtagcag gagaggcttt ctgactgccc taaccccgga
    18901 cgttccccca ccagcaggat caccactttc gcgccgagga tctcaccgat ctgatcttca
    18961 atcttcacgc gaccataacg aacaaagaat ggcgtgccga ctttcagccc ggcctgtttc
    19021 aggcccgcca tcagcggcgg caggatctct tcgtagttga cggtgatggc atcagttgac
    19081 aggccatcag aaataacgac ctgtacgtcc ggattagcaa cacactgtgc tttcagcgct
    19141 tcaacagctt ctgcacacag gcggcggccc atatccgggc gagtcaggta gagatttttg
    19201 tcgctaattt cagagcgtac ttccagcaag ccctgcgctt tcacccactc ttccggcact
    19261 tctttcagta cggtatcttt cgaacgagag tgatcggcca ggaaacgcag cagcgcctga
    19321 gtacgtggac gcggaccggc acgaccggta caaacgcggg ccacggtgct gcggcgcagt
    19381 tctgttaata cgtctgcgcg atgcgggttt tcaacaccaa tccacgcttt tgcttcagcg
    19441 gaacctaaat ccagcgcaca gctttccgag gtcaccggtg ccgcacagtt ggtggtggcg
    19501 cactttgctt ctgacggcgc gggggccgct tgtcccattg acgccatcac gctgcgtaca
    19561 atttcttcaa tctgtttttg atccatgatg tgttatctcc gcgtcatcag aagaacagtg
    19621 acggatcgcc cgcccgtttg gtcaggcgac cgtttgccat aatgcccatg ctttccagcc
    19681 agcgttcaaa ctctggtgac gggcgcaggt tgagtaactg acgcacagtg gcggtatcgt
    19741 ggaatgcggt ggtctggtag ttgagcatga tgtcatcacc cagcggcatc cccatgatgt
    19801 agttgcagcc tgcggtggcg agcaggatca tcaggttttc gttgaggttc tggtcagagt
    19861 cagcgtggtt ggtgtagcag cagtcacagc ccatagagat gccgctcagc ttgcccataa
    19921 agtgatcttc taagccagca cggataatct ggcggtcgtt gtagagatac tccggcccaa
    19981 taaagccgac cacggtgttg acgataaacg gatcgtaatg acgcgccagc ccatagttac
    20041 gtgcttccat cgtcacctgg tcagcgccga agttagcgcc agcggatagc gcagagcctt
    20101 gtccggtttc gaagtagagg cagttttccc cggcgatacg attgaactct gcgcccactg
    20161 cgcgcgcttc gtcgagcatc gccagttcca cgccaaactc tttcagccct ttttcgctgc
    20221 cacagatact ctggaaaatc agtccgccag gcgcgccgcg acgaatcgct tcgatctggg
    20281 tggtgacgtg cgccagtacg cagccctgag ttgggatgtt gaatttgtcg atcacgccat
    20341 aaatggtatc cagcacgcgg cttaagtttt ccacgtcgtc agttaccggg ttaacgccga
    20401 tcaccgcatc gcccaccccg aaggaaagcc cttcatagat ttgcgcagcg atactttgca
    20461 cgtcgtcacg ggtatcgttc ggctgcaaac gggcgctaaa ggtgcccgga ataccgatgg
    20521 tggtattggc ctttttgatt accggcattt tcttcgcgcc gtagatcagg tccgcgttgg
    20581 agcaaatctt cgctaccgcc gcgaccactt ccgaggtcag ccctttgcgg gtaaaggcaa
    20641 tgtcgtccac gctggtttca tcgctcagca catactcacg cagttcgctg atgctccagt
    20701 ttttaatctg gttgtaggcc gtttcgttaa cgtcgtcctg aatcagccgc gtcacgcagt
    20761 catcttcata ggcaatcacc ggattattgc ggatgtccgc tacggtcatt tccgacaaca
    20821 cctgctttgc cgccacgcgc tcctgtgagc ttgccgctgc aacgcccgcc agcacatccc
    20881 ccgaacgcag ttcgttggct ttagccagca cctcttttac atccttaaac tgatatacat
    20941 tgccgaacaa tgtggtcttt agtttcataa gtcgttccct caggaagaag tgatgagtaa
    21001 catttcttcc agcattcgaa caattctatt tcatcgttag cccttgtcct gtaaggtgtt
    21061 acccttaata cgcccgttac ttatcctgcc ggacacaacc gaacgcgccg gatacatgag
    21121 cggggttatc gagtcgttag atcgaggtat ccggtttgag caagacacca ctgacagcaa
    21181 aggccataga ggccgcccag ccgcaggaca agccctacaa actcactgat tcaatgacgc
    21241 ctggcctttt cttgctggta catcccaacg gcagcaaata ctggcgattc cggtattgga
    21301 tagacaagaa ggagcggtta caggctattg gggtgtatcc gctgattagc ctcaaggaag
    21361 cccgcaaacg cgccacagag agccgtttac tgatagccca gggaattgac ccaatggatt
    21421 tggcacgcaa ggagaaagcc attgatgctc ttaatgctat cgccagtttt aaagctgtag
    21481 cagaagactg gatggctacc aaggtgagcg gatggagtga gagctataca aagcaggtca
    21541 gatcggcact agagaaagat gtttaccccg tgctaggtaa gcggtcgata gtggatataa
    21601 cggcccgtga cgtgctggcg ttactacaga aaaaggagcg caccgcaccg gaacaagcca
    21661 ggaaattacg ccagcgtatc ggggagatct tcaaatttgc cgttatcact gaactggtta
    21721 accggaatcc ggttgcagat ctggatacgg cactgaaagc cagacgccca ggccataacg
    21781 cgtggatacc gattaatgaa attccggcct tctacaaagc ccttgagaga gctgggagcg
    21841 ttcagattca gacggcaata cttttgctca tcctcacagc tttgaggacg gcagaattgc
    21901 gcctgatgcg ctgggaatgg gtagatctgc aatcggcaac aatcacattg cccgctgaag
    21961 tcatgaaggc ccgccgaccg catgttgtcc cgctatcccg gcaggcggtc gagctattac
    22021 aggaccagtt tacccgcagc ggatacagtg ctttcgtctt tcctggccga ttcatggaca
    22081 agccactttc agccagtgcc atccttaaag ccctggagcg tatcggatac aagtcgatag
    22141 ccacaggcca cggctggcgg accacctttt caacggcgct taatgagtcc ggcaggtact
    22201 caccagactg gatcgaaatc caactggccc acgttccgaa aggcgtgcgc ggtgtttata
    22261 accaggcggc ctacctgaag caacgacggg ccatgatgca ggattatagc gactgtattg
    22321 attcaatact ggctggtgac ggcaatccgc ttgagccgga atgaataatt aagcgggttt
    22381 ccgacctact ccaccggagc agactagata aatactggac cccttcagaa taaatattca
    22441 cctgccggat tcgtccggct tttgtttata aatcatccaa tcgtgaaatt attagaatta
    22501 aacttacata ctagtttact agccagatta ggctactagt atataacatc aataaaatca
    22561 tgaggttagt caaaagttgc cgtcaggggt catgcgggcg taacattaga tcaacgaaat
    22621 aaaaaagccc ggaaaggcgg caaccagttc cgggcggtat gagaaacaag ctaaaggaga
    22681 atttctcatg tctgatatgc tagcagtaga acaagaaaca aacaatgatg ttcgccaata
    22741 tttaaacaaa attaacgagt tacggaataa agcacccaaa aacgaagaga ctaaacacga
    22801 agaacacacg ccagatcatt acgaggcaaa acagcaggag caagcctggc gtggcaacct
    22861 gcgctacctg gacacattaa accgacttga tgaggtattg cctcgcaaac tgtacgaaag
    22921 gtgggagaaa gaacatacag tcaacgacga ggccgtgcta cgcgcccttt gctactttgc
    22981 tggtactggc aaaaactcac agttgggatg gtgcagggtt ggcaggggaa caatcgacaa
    23041 gcgagccagg ctatcaaaaa acacggttaa aaaatgcctc gatcgtctgg tccaccactt
    23101 caaactggtt gagcgtactg aaggctatat acccggatcg gcagaaaggg aatgcaacga
    23161 gtaccagcta ttattcaagc cgtacaacat gaaataacgg aatcaggcgg gcaatgttcc
    23221 cgccttttct ttgccttaat tccatgaatt catgggtggg tcaaaggttg ccgttagggt
    23281 aggtcaattt gtgcctttag gtgggtcaaa agctgccgtt aaggtgggtc aaaagttgcc
    23341 gagtaaaaat ttcactatta agaattctag gcgacgggag ttttttagcc tcccggtcgg
    23401 tcacagagag tcgcctagga ttaaaacaga agaatttatt gccgcgcagc gctggatgaa
    23461 tgcaatcatc aataccgctt tggcgcgtca tccttcccag caagctggaa aggcccattc
    23521 actcacgcac aacctgcaaa ccagaccaac agaccatcat ggcgattcgt tcatgggtag
    23581 cgcgggtata tctcaaaggg tgatttcttc ttggtggacc ttcggaagac ttcgagcaat
    23641 tgccagctta aagacagctt agcagtgatg gttatcatca acagctttct gagctaccgc
    23701 acagacacac agcagcagta agcaaaccga agggaatgca aagcaaaagg attcgccaca
    23761 ctgcaagacc aatggttttt gtcacgtata cagaccaggc aagcaacagc agcaaagcag
    23821 cgtatagcca ggtgcagata gtaccatcac acaacgcaca gcaggctgag gtgaacactc
    23881 aagcaacttt atccccgcca ccgaccaccg caaactatcc tgaccgtgcc aaattcgaac
    23941 gtaatgccac ctggaacgcg atggagacac ataagggcta cgcttgctta gctgaaaact
    24001 tcacagcgtc acagcgctat ttagacgcat tagagtcgat gcgatttcat taaatttttt
    24061 agccatccag aaatcggcct gattctgagc cgattgccag ccggactact gaccagatcg
    24121 gaccaatcat ccaaggttgg atgaatgaaa tcacacgcag atcctgccag gctatttcat
    24181 cccaccacac agaacgctga gatctgatat taccgccgca ccattccacg ccagacgaat
    24241 caaaacaggc ttacccttcc aagtggatag ctaaaaatta aggctgtcag aatgcgttat
    24301 attgcgttta aacggcatcg aattttaatg tgcgcaatgg tgccagattg caggcaaaaa
    24361 aaatcccgcc ggagaaagcg gggaagtgaa atgaatcgtt gtagttatag atcacatttt
    24421 gagtaagggt aaccagccct tgagtaatat tatccctgca ttacgtgaga tgcgcaatta
    24481 ttttttgcta attaaaagtt ttgtaaacat tacagaatat cccctttcac tatatggtaa
    24541 aagatgtgaa acagggacag gaggaatata atattaagta tcaacagtaa tgttggcctt
    24601 taatttcatt cagggcgcgg tgagggtttt ctccatattt tcctcaccgc tactttttga
    24661 ggtgagaaca tgaatgatag cgaattaatg acggttaacg aggtagcagc tttattcggc
    24721 gttacccgtc ggacaatatt ccggtggatg aacaagatta aaggctggcc cgtcccggtc
    24781 agcccgattg actcaagaat taagtttatc cgatctgaga ttttggaatt ctataaaaac
    24841 aaaggcgctc gccaccaatg aaaacagcta ctgagattat caaataacct attgatttaa
    24901 aaagctagct accgcaattt ctgaaggaat aggcagcatg aaaatcaaag caaacacaat
    24961 taaagaaata acatcaatca aagtaaaagg agcagatgcc atgcattcat tagacagtta
    25021 ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg aattccagga
    25081 aaaagtgatg cgttctgctg attatattgc ggacaagttc gtggaaacgg ttcggccact
    25141 tgttgatgaa gtagctgata aacttcagtc tgaaatgccg gaagacatgg aaggcactgc
    25201 aaaggcgcga ctcctttttg aattaagtcg tcgtttcggc gtgtcaataa gcacttttaa
    25261 ataatcggag gaggaaagac aatggacgca tttgatgtac tgacaaacaa aggcgatgaa
    25321 ctggtagcac agcgtgataa aaccgccaac aaaggggaac gcgcactgtt aaataagcag
    25381 attaaagcga tccgtatggc ccagtttaaa ttaatcagca atgaagttat cgaaataatt
    25441 aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact ggagaaacgc
    25501 attgccgggc tgaaaacgaa cgtagagaaa atggagatgc acaaacagag cggtgctccg
    25561 actccggatg agtacttctc accggatgct gagtttatgg cgcaggttga ggaacgtctg
    25621 ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag ttcggcaggt
    25681 attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc cccgtctatg
    25741 ccggagccag aaccagaaat ggccgccgat cctgaagctg agcaacggaa acgcgtattc
    25801 aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac cccggctgac
    25861 ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag tctcgaaaca
    25921 atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc actacgcatc
    25981 atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca ttcgccactg
    26041 agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc gcttggaact
    26101 atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga ggaaatggag
    26161 ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca cagcgtggga
    26221 atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc gcgcgcgagg
    26281 agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc cagcgggcag
    26341 aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa gcagagttac
    26401 taaccaaata catcagtttc accaattact gcatctacga gtgttacaag gaagatgcag
    26461 aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac gtcatgttgt
    26521 tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca cttcagcatc
    26581 accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg taggaataga
    26641 ccaggttggc attgaaacac atctaccatg accataataa agtaattttt tgatatgttt
    26701 atggttttat attaattaaa atatatctaa aattgatagt tttaaaattg caaaatccag
    26761 attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga aaaataaatc
    26821 aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc tttcatttat
    26881 attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat tcagcgccat
    26941 acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt tctgtcaaac
    27001 atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa acatgattta
    27061 taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta tgctttccga
    27121 ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg gttcatctga
    27181 agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac gcgaagtaca
    27241 aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat aaattctgcc
    27301 gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg ataatgaatg
    27361 ctaaagaggt tttgaagcat gtcgatgaaa aaactattgg tgtcgttacg actttaggta
    27421 ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt aaagttaata
    27481 ttagttttta tatttacatc gtaattatat gtttctattt taaaaatatt cgagagtata
    27541 aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg gacactcgct
    27601 atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc agaatacggc
    27661 ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc tgttgccttt
    27721 gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg atggatagga
    27781 gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct acaagtcatc
    27841 ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat tgatgttgat
    27901 atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt cgtactattt
    27961 gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc gcaggtatct
    28021 aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat tcttggtttc
    28081 tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg tgatcttttc
    28141 cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct tgcttatgca
    28201 gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa aaaataccca
    28261 attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag tactttaggg
    28321 gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt ggttgcctac
    28381 gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc atttatgcta
    28441 gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac aggtgtatta
    28501 gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg tcatggaatg
    28561 gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt tacttttgtg
    28621 ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt tatgctctat
    28681 ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc tcaacctgat
    28741 acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg attcttaggg
    28801 tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac tggtagtcca
    28861 accagctatg ttctttattt gttagtatta gttgccctat ttttattaat tccaatatta
    28921 atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga accattttct
    28981 tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag gttaaatatg
    29041 tttagtgctg aatctccaat tggtgtaata gactcaggtg taggtggatt gacatcggta
    29101 aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga taatatgaat
    29161 gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa aatgctgact
    29221 tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat atcctcaact
    29281 ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat cttatccatc
    29341 attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg tattatcgct
    29401 accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt gaactcaaca
    29461 ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga aggtaatctt
    29521 aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat ttcccttcat
    29581 ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa tttttttgaa
    29641 gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat ctctattaaa
    29701 aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt gcaaataaat
    29761 actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat tacgaaacct
    29821 catacattca gtatcaggca gttttaaatt tatccggtgc accgtaaaac cccgtccttc
    29881 tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg gacaaaccca
    29941 gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata aacgctccga
    30001 tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga cccaaccgct
    30061 taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat attttgccgg
    30121 tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg ctggtcagca
    30181 tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga tcggttcggg
    30241 gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac ccgagccagt
    30301 gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag ataaacgcca
    30361 catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt ttaaagtccg
    30421 gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc cggcaccatt
    30481 aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat aagtataagt
    30541 gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt tagggttctg
    30601 accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa cgctggtcat
    30661 gagataacct caaaagcccg tattatacat cagattcaac taattagatg catcacccat
    30721 ccagcattat aaaacatact aacaataact aaattataat aatcacaact tacaatcaaa
    30781 taataaatgc ataagattaa caatgatttt ttctggttat atttatctaa aaaagcagaa
    30841 tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac taacaaactt
    30901 gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg tttttatttt
    30961 tcattttctc ttttcttctc tttccggttc tcttcttttc tggttttggt tttttccttt
    31021 ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc accacaaaag
    31081 catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga tataatccca
    31141 ctaacaccca gccaccaagc accatacctg ataactaccg tattcgggaa ggaaatgcga
    31201 gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca atgtcgatat
    31261 agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg
    31321 ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc agcaggggat
    31381 gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc agtaccgcgg
    31441 cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cgcgtcagtt ttgggatcaa
    31501 gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca tcaatcggga
    31561 tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt gtgctgccag
    31621 agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc gcgtacggtt tgcgccggaa
    31681 actgcacatt catctcacgc aagcgcggat ggtcatgcag cgccgttgcc agaagtgggc
    31741 caatatcggc aaaacagaac gggtcggcgg gctggtggcg ataacattcg cccacgcctc
    31801 cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg
    31861 cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc gccatccgcc
    31921 gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc
    31981 actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg
    32041 tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt ccggcatcga
    32101 acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga cacagccgtt
    32161 gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg aggtgcggcc
    32221 cggcgctggc gacgacaaaa tcgcccagcg actgagagag cgccatcacc gccgggcgag
    32281 cattgcgggt tttcgcgctt tcaccggtga tgatgatggc accagaatca acgctttccg
    32341 gcgcaacgcc cgcagcctga tattgctcta aaatcagcga tttcagttcc gcttctttta
    32401 aaccaccctg tttatcgaca ggggtaaaga acaccgggct ttgccagcta atttcgcgtt
    32461 taatgaattc gtagcgcggc acctgcgaca ccgccgcacg gttaaccagc tccaggtggg
    32521 agaaaatcac ctgggtggtg gtggtgccga tatcgatacc gacgctcaat agctggcgag
    32581 tgttcacgat tgtgcctccg cttcggtttt agtcgcggtc gcgtcttctt ttggcaccag
    32641 catcatcgcc acgccaatcg ccgttacgcc gccgatcaac ttgccgacaa tcatcgggaa
    32701 gatcatggcg ttcatgttgg cagcggcgaa gcctaagtgg tcgcccaggg cgaaagcagc
    32761 ggaaacggcg aaggcgcagt tgatgacttt gccgcgggta tccatctgcc tcatcatgcc
    32821 gaacatcggg atgttgttgg caagcgttgc caccatgccg gctgccgcga tgttgttcat
    32881 attcagcact ttaccgacgc tcatcagcgg tttttcaaac cagcgggtca gcagcagcac
    32941 cattggatac gcccctaaca gaacgcagga gatagaaccg ataacttcaa tggcgcgcat
    33001 cacttcaccg ggtttatcgc caggggccat aaagataggg tccagaccgg ggatcagttc
    33061 ccagccaagc agaaacttaa ctaccgcggc agcaagaccg agggtgatca atgcaacgag
    33121 gaatttggcg aagatctgga agccgttgat cattttttcc gggatgaatt tcagccccag
    33181 cgccaccagt accgcgacga taagcaccgg gatcatgttc atcaggatga gcgcaaaggt
    33241 gaattccacc ggctggccgt tgatctgtac accggagtac atagccacca ggccaccggc
    33301 gatacagcca attggaatgg tcacaatgcc cgccagcacg ccgagcgcca gataacgacg
    33361 atcagaaggt tcgataatgc cgagcgccac cggaatggaa aacacaatcg ttggccccat
    33421 catcgacccg agaattaacc cagagtatag ccacgctgct acgtcgccgc ccgccagctc
    33481 tttggcgagg aagaagccgc ccatatcgca cgccagcagt gttccggcga acattgacgg
    33541 gtttgcaccg agcatttcgt aaaccgggat aattaccggc ccgagtacgt gagccagtac
    33601 cggtgccagc gcggtcatac cgaccatcgc cagacccaga gcgcccattg ccataaagcc
    33661 ttcttcgaac tgaccgcctg atccttcgat acttttaccg aacttaccga ggaaacgagc
    33721 agaaccgccg aactgcgaca ggatcctgtc tacggcagct atcagcataa agaacatcat
    33781 gatgtacatg atgatttcgt taattcccat cgcctttact ccctgttagt tgttatttat
    33841 tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg caatcccgaa ggccggatca
    33901 gacgcggcaa gc
//
LOCUS       DADKXM010000028        33908 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.028, whole genome shotgun sequence.
ACCESSION   DADKXM010000028 DADKXM010000000
VERSION     DADKXM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.028"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..563
                     /locus_tag="JQR05_001349"
     CDS             <1..563
                     /locus_tag="JQR05_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323552.1"
                     /translation="DIIISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMR
                     GLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEML
                     GMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLE
                     IKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(623..1114)
                     /gene="yajQ"
                     /locus_tag="JQR05_001350"
     CDS             complement(623..1114)
                     /gene="yajQ"
                     /locus_tag="JQR05_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY5323553.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1282..2193
                     /gene="panE"
                     /locus_tag="JQR05_001351"
     CDS             1282..2193
                     /gene="panE"
                     /locus_tag="JQR05_001351"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY5323554.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2156..2746
                     /gene="yajL"
                     /locus_tag="JQR05_001352"
     CDS             2156..2746
                     /gene="yajL"
                     /locus_tag="JQR05_001352"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY5323555.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2800..4248)
                     /gene="thiI"
                     /locus_tag="JQR05_001353"
     CDS             complement(2800..4248)
                     /gene="thiI"
                     /locus_tag="JQR05_001353"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY5323556.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4454..4696
                     /gene="xseB"
                     /locus_tag="JQR05_001354"
     CDS             4454..4696
                     /gene="xseB"
                     /locus_tag="JQR05_001354"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY5323557.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4696..5595
                     /gene="ispA"
                     /locus_tag="JQR05_001355"
     CDS             4696..5595
                     /gene="ispA"
                     /locus_tag="JQR05_001355"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY5323558.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5620..7482
                     /gene="dxs"
                     /locus_tag="JQR05_001356"
     CDS             5620..7482
                     /gene="dxs"
                     /locus_tag="JQR05_001356"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY5323559.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSCKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7537..8511
                     /gene="yajO"
                     /locus_tag="JQR05_001357"
     CDS             7537..8511
                     /gene="yajO"
                     /locus_tag="JQR05_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY5323560.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(8565..9083)
                     /gene="pgpA"
                     /locus_tag="JQR05_001358"
     CDS             complement(8565..9083)
                     /gene="pgpA"
                     /locus_tag="JQR05_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY5323561.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLDILS"
     gene            complement(9061..10038)
                     /gene="thiL"
                     /locus_tag="JQR05_001359"
     CDS             complement(9061..10038)
                     /gene="thiL"
                     /locus_tag="JQR05_001359"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY5323562.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(10116..10535)
                     /gene="nusB"
                     /locus_tag="JQR05_001360"
     CDS             complement(10116..10535)
                     /gene="nusB"
                     /locus_tag="JQR05_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY5323563.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10555..11025)
                     /gene="ribE"
                     /locus_tag="JQR05_001361"
     CDS             complement(10555..11025)
                     /gene="ribE"
                     /locus_tag="JQR05_001361"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY5323564.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLE
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11114..12217)
                     /gene="ribD"
                     /locus_tag="JQR05_001362"
     CDS             complement(11114..12217)
                     /gene="ribD"
                     /locus_tag="JQR05_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY5323565.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12221..12670)
                     /gene="nrdR"
                     /locus_tag="JQR05_001363"
     CDS             complement(12221..12670)
                     /gene="nrdR"
                     /locus_tag="JQR05_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY5323566.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12821..13351
                     /locus_tag="JQR05_001364"
     CDS             12821..13351
                     /locus_tag="JQR05_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY5323567.1"
                     /translation="MNTNVFRLLLLGSLSACVQQSEVRQMKHSVSTLNQEMTQLNQET
                     VKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLRIQG
                     ESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKGISV
                     DQLGFVRIHDIQPVMQ"
     gene            13650..14534
                     /locus_tag="JQR05_001365"
     CDS             13650..14534
                     /locus_tag="JQR05_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY5323568.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14711..15058)
                     /locus_tag="JQR05_001366"
     CDS             complement(14711..15058)
                     /locus_tag="JQR05_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY5323569.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15187..16158)
                     /gene="secF"
                     /locus_tag="JQR05_001367"
     CDS             complement(15187..16158)
                     /gene="secF"
                     /locus_tag="JQR05_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY5323570.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFCKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16169..18016)
                     /gene="secD"
                     /locus_tag="JQR05_001368"
     CDS             complement(16169..18016)
                     /gene="secD"
                     /locus_tag="JQR05_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY5323571.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18044..18376)
                     /gene="yajC"
                     /locus_tag="JQR05_001369"
     CDS             complement(18044..18376)
                     /gene="yajC"
                     /locus_tag="JQR05_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY5323572.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18399..19526)
                     /gene="tgt"
                     /locus_tag="JQR05_001370"
     CDS             complement(18399..19526)
                     /gene="tgt"
                     /locus_tag="JQR05_001370"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY5323573.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19581..20651)
                     /gene="queA"
                     /locus_tag="JQR05_001371"
     CDS             complement(19581..20651)
                     /gene="queA"
                     /locus_tag="JQR05_001371"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY5323574.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESSAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20744..21325
                     /gene="acpH"
                     /locus_tag="JQR05_001372"
     CDS             20744..21325
                     /gene="acpH"
                     /locus_tag="JQR05_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY5323575.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(21330..23147)
                     /gene="malZ"
                     /locus_tag="JQR05_001373"
     CDS             complement(21330..23147)
                     /gene="malZ"
                     /locus_tag="JQR05_001373"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY5323576.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23303..24676)
                     /gene="proY"
                     /locus_tag="JQR05_001374"
     CDS             complement(23303..24676)
                     /gene="proY"
                     /locus_tag="JQR05_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY5323577.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGVGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24752..26071)
                     /gene="brnQ"
                     /locus_tag="JQR05_001375"
     CDS             complement(24752..26071)
                     /gene="brnQ"
                     /locus_tag="JQR05_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY5323578.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26478..27773)
                     /gene="phoR"
                     /locus_tag="JQR05_001376"
     CDS             complement(26478..27773)
                     /gene="phoR"
                     /locus_tag="JQR05_001376"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY5323579.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27831..28520)
                     /gene="phoB"
                     /locus_tag="JQR05_001377"
     CDS             complement(27831..28520)
                     /gene="phoB"
                     /locus_tag="JQR05_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY5323580.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28710..29912
                     /gene="sbcD"
                     /locus_tag="JQR05_001378"
     CDS             28710..29912
                     /gene="sbcD"
                     /locus_tag="JQR05_001378"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY5323581.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            29909..33052
                     /gene="sbcC"
                     /locus_tag="JQR05_001379"
     CDS             29909..33052
                     /gene="sbcC"
                     /locus_tag="JQR05_001379"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY5323582.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33178..>33908
                     /locus_tag="JQR05_001380"
     CDS             33178..>33908
                     /locus_tag="JQR05_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5323583.1"
                     /translation="MKKVILSLALGTFGLVMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FCYVKPYMMFISGFSETAMTFIMMLV"
BASE COUNT         8544 a         9104 c         8551 g         7709 t
ORIGIN      
        1 gggatataat aataagtaat actggtgaaa aatttagtga agtgggtcta accaaatatg
       61 ctcaacatct cgcatctttc gctgaagaat tttatataaa agagcaaaaa tcacattctg
      121 aaagtgaaat gagaggacta ttttcatcac ttgccgaatt atatagtttt ggtaatgata
      181 ctgccagcgc aaaccgagtg ttccatcagc atgtccctgc cttcaataca gatcatatga
      241 ttcaatattt tataaatgcc aaagaatgga acaatgccag ggaattattg ataaaagagg
      301 aaatgttggg tatgcataat ctgatattgt tggagaatat ttgtatgcaa aaaaatgcag
      361 aatgcatggc acacattacc tttacgctta ataaattaac aacacaacca gcaataacaa
      421 aaatagatag cgtgggtaat gagcaacttt atcagattgg caaaatctac cataagttgg
      481 aaattaaacc tgagccagag caacaggttt taattcagtc gctttatgac cgggcatcag
      541 gaagcgcctc tgcaactcaa taaatgcatg gtcaaaaggg ccaccacggt ggccctgaca
      601 atcagtagct atatccagaa agttaatcgc ggaagttttt gaactggaac ggctgaccga
      661 gatcgccacc gcgcaccatc gccatgacag cctgcaaatc atcacgagat ttgccagtca
      721 cgcggatctc atcgccctga atttgcgcct gcactttcag tttgctgtct ttgatcatct
      781 tgacgatttt tttctgagtg gcgctctcaa tgccctgttt cagtttcgct tccacaaacc
      841 aggttttacc gctatgaacg atattttccg gtacatccag tgaactgcct tcaatgccgc
      901 gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaag tcggactcgc
      961 tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca acgttacgga
     1021 agtcaaaacg ggactccact tcgcgactcg cgttatcgac cgcgttacgt gcttcctgaa
     1081 gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc atttttgatg
     1141 cgaagcataa tacctgcaaa gttaaataac caacagtcct gcaccaggac gtatccctta
     1201 attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa cacccggagt
     1261 ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc cttagggcaa
     1321 ttatggctta cagcactttg taaacagggt catgaagttc agggctggct gcgcgtaccg
     1381 caaccttatt gtagtgtgaa tctggttgag acagatggtt cgatatttaa cgaatcgctg
     1441 accgccaacg atcccgattt tctcgccacc agcgatctgc tcctggtgac gctgaaagca
     1501 tggcaggttt ccgatgccgt caaaagcctc gcatccacac tgcctgtaac tacgccaata
     1561 ctgttaattc acaacggcat gggcaccatc gaagaattgc aaaacattca gcagccatta
     1621 ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat tcatgtggca
     1681 aacggaatca cgcatatcgg tccggcacgg caacaggatg gcgattacag ttatctggcg
     1741 gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg cgccgagctg
     1801 tggcgcaagc tggccgtcaa ctgcgtgatt aatccactga ctgccatctg gaattgcccg
     1861 aacggtgaat tacgtcatca tccgcaagaa attatgcaaa tatgcgaaga agtcgcggca
     1921 gtgatcgaac gcgaagggca tcatacttca gcggaagatt tgcgtgatta cgtgatgcag
     1981 gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc
     2041 cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc gcatgggatt
     2101 gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag
     2161 cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg tcaccacgat
     2221 cgacctcttg gttcgcggcg gtatcaaagt caccactgcc agcgtcgaca gcgatggcaa
     2281 cctggcaatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaagt
     2341 ggctgatggc gaatatgacg tgatcgtgct gcctggtggc attaaaggca cggagtgttt
     2401 tcgcgatagc actctgctgg ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt
     2461 cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct ttccgattgg
     2521 caatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat ggcaggacaa
     2581 gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg gtacagctat
     2641 cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc atgaagtggc
     2701 atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc ggatgcggca
     2761 aacgtcgcat ctgaccagat gcgacgtaaa aactacaaat tacgggcgat ataccttcac
     2821 attgttaaag ccttgctcgc gcagatagag cgcctgcaga cggctcatca ccccgcgctc
     2881 acaccacagc agccaggttt tgttctggtc gagatcgcca aatttggtgc tcagtttata
     2941 gaacggcaga gaaaccacat caatcccttc gactttcagt ggcttatctt cctgttcatc
     3001 gacagaacgg atatcgagaa tcacgtcgtt cgggccgaag ccgttgacgg tttccacttc
     3061 caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat tcgcttcctc
     3121 aaccacttta tcgagaatac tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt
     3181 aaccgctttc accgtcgggc ttttggagat cacgccacaa tattccggca tcgtgcgagc
     3241 aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt cgtaagaaat
     3301 cagcggacgc aggatcagcg tgtcagagac gttatcaatc aggcgcaggt tggtcagcgt
     3361 ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc
     3421 agccacttta gatgcggcac gcaccatcat acgtttgagg ataacgccca tctgaccgtc
     3481 gtcgattttc tcgagaattt ccccgacgac cggttcgaaa ttaatggcga caaaacgcac
     3541 gcggtgggag ctgccaaagc ggttccacag ataatgcgcc acctgacgca cgccaatttc
     3601 atgcgctgcg ccgccgaggt taaagaagca gtaatgcacg cggcagccgc gacgcatcaa
     3661 catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca catcttcctg
     3721 ggtgccaatc gggaaaccac caataccttc gtagcgacct ttaatcagca ggaggcgatc
     3781 gtcttccact tccagatgaa cagttacatc cgggttagtc agcttcacgc gcgcggattc
     3841 aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc taaaatcatg
     3901 tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat cgcgatactg
     3961 aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag
     4021 aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagtcagac gctggttttc
     4081 atctttcgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg tctcatcata
     4141 gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca agcgcacaga
     4201 ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg cggcaatggt
     4261 tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac acctgcggct
     4321 gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca ccttaccatt
     4381 gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa atattgagag
     4441 tcagacattc attatgccga agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag
     4501 cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg aagaggcgct
     4561 gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat tacaacaagc
     4621 cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa ccccttttac
     4681 accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa gcaggccaac
     4741 caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt ggtcgaaacc
     4801 atgcagtatg gcgcattatt aggtggtaag cgcctgcgac ctttcctggt ttatgccacc
     4861 ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgctgc cgtagagtgt
     4921 atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga cgatctgcgt
     4981 cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac
     5041 gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga agtgtcggat
     5101 cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc cggaatgtgc
     5161 ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga cgcgcttgag
     5221 cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct tggtgcatta
     5281 agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtatgc agagagcatc
     5341 ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac tgcaacgttg
     5401 ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc acttctgggt
     5461 cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca gtcgctgaaa
     5521 caactggctg aacagtcact cgatacctcg gcactggaag cgctagcgga ctacatcatc
     5581 cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga tattgccaaa
     5641 tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc gaaagagagt
     5701 ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag ccgttccagc
     5761 gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca ctatgtctac
     5821 aacaccccat ttgaccaatt gatttgggat gtgggacatc aggcttatcc gcataaaatt
     5881 ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggtct gcacccgttc
     5941 ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc aacctccatc
     6001 agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg ccgcaccgtc
     6061 tgtgtgattg gcgacggcgc gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg
     6121 ggcgatattc gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa
     6181 aatgttggcg cgcttaacaa ccatctggct cagctgcttt ccggtaagct ttactcttca
     6241 ctgcgcgaag gcgggaaaaa agttttctct ggcgtgccgc ccattaaaga gctgctcaaa
     6301 cgtaccgaag aacatattaa aggcatggtg gtgcctggca cgttgtttga agagctggga
     6361 tttaactaca tcggcccggt ggacggtcac gatgtgctgg ggcttatcac cacgctaaag
     6421 aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt
     6481 tatgaaccgg cagaaaaaga cccgatcact ttccacgccg tgcctaaatt tgatccctcc
     6541 agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat ctttggcgac
     6601 tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc ggcgatgcgt
     6661 gaaggttccg gcatggtcga gttttcatgt aaattcccgg atcgttactt cgacgtggct
     6721 attgccgagc aacacgcagt gacctttgcc gccggtctgg cgattggtgg atacaaaccc
     6781 attgtcgcga tctactccac cttcctgcaa cgcgcctatg atcaggtgct acatgacgtg
     6841 gcaattcaaa agctaccggt cctgttcgcc attgaccgcg cgggcattgt tggtgctgac
     6901 ggtcaaactc accagggcgc ttttgatctc tcttacctgc gctgcatacc agaaatggtc
     6961 attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg ctatcactat
     7021 aacgatggcc cgtccgcggt gcgctacccg cgcggcaacg cggttggcgt ggaactgacg
     7081 ccgctggaaa aactaccaat tggcaaaggc attgtgaagc gtcgtggcga gaaactggtg
     7141 atccttaact ttggtacgct gatgccagaa gcggccaaag tcgccgaatc gctgaacgct
     7201 acgctggtcg atatgcgttt tgtgaaacca cttgatgaag cgttaattct ggaaatggcc
     7261 gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg cgcaggtagc
     7321 ggagtgaacg aagtgctgat ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg
     7381 ccggacttct ttattccgca aggaactcag gaagaaatgc gcgccgaact cggcctcgat
     7441 gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact ccactcctgc
     7501 tatgcttaag aaattattca tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa
     7561 accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc
     7621 ggtaatcacg catggacact gccggaagag agcagccgtc ccataattaa acgcgcgctg
     7681 gaaggtggca taaatttctt tgataccgcc aatagctatt ccgatggtag cagcgaagag
     7741 atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtagtcgt tgccaccaaa
     7801 gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct
     7861 atcaacgaca gcctgcgacg tctcggcatg gattatgtcg atatcctgca aattcatcgc
     7921 tgggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc
     7981 gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggcgctg
     8041 gaactacaaa aacagcactg ctgggcgcag tttgtcagta tgcaggatca ctataatctg
     8101 atttatcgcg aagaagagcg cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg
     8161 attccgtgga gcccgctggc gcgggggcga ctgacgcgtc catggggaga aactaccgca
     8221 cgactggtgt ctgatgaggt ggggaaaaat ctctataaag aaagcgatga aaatgacgcg
     8281 cagatcgcag agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt
     8341 gcgctggcct ggttgttaag taaaccgggc attgccgcac cgattatcgg aacttcgcgg
     8401 gaagaacagc ttgatgagct gctgaacgcg gtggatatca ctttgaagcc agaacagatt
     8461 gccgagctgg aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg
     8521 cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc acaactacga cagaatatcc
     8581 agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaatcacccc ggcgacaata
     8641 tcgtcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac
     8701 ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca
     8761 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
     8821 ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag
     8881 atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag
     8941 aaagggatcg ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg
     9001 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
     9061 ttatggcgtg gcaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg
     9121 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
     9181 aaggtgtccg agagcaacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa
     9241 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
     9301 agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc
     9361 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
     9421 cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat ggacggagat gacgtttgat
     9481 caagtagtca gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
     9541 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccaga
     9601 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg
     9661 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
     9721 actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt
     9781 cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt
     9841 ataagccaga tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca ccagcgtatc
     9901 agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat cgtcgccgat
     9961 acccagttcg acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga
    10021 gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca
    10081 tggaacagtc ttccgtgaat ctaccggcct ggatattact ttttgttagg gcgaatcaca
    10141 ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat
    10201 ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcact acgtttagac
    10261 agttcgtaca gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg
    10321 cgggacaggt agggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc
    10381 agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc caggaactgg
    10441 tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca
    10501 cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc
    10561 cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt
    10621 gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac
    10681 cccaaaagca accggaattt cgctgtcctg ggcaacatgc tccaggccgt tgcttgcacc
    10741 accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca gcgcaatcac
    10801 cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc
    10861 aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc
    10921 aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc
    10981 gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt
    11041 tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt
    11101 tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct acatgacgta
    11161 tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc agcgagcata
    11221 atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc tcatcgacta
    11281 aacccgcctg tagcaatacg ccagcgagcg ttggccccgc ttccacccag atgctgttaa
    11341 tctgctgttt acccagttgc atcatcagta caaccagatc cagatgacct ttatgctctg
    11401 gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga
    11461 accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat
    11521 caatcacaat acgtactggc tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt
    11581 catccagttc agaccaacgc actgttaagg ctggatcatc cgccagcacc gtggcgctgc
    11641 tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg
    11701 aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg gccatcaagc gatgcgccaa
    11761 gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt
    11821 gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt tgcagacggt
    11881 aaagtccacg cccagcgacc tgcgggttag gatcttgcat cgctgcgacc acgcgcgcta
    11941 cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg gccatgatgg ctacagggtt
    12001 cgagtgtcac atacgccgtt gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta
    12061 cttcggcatg tggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt
    12121 tgacaatgac gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca
    12181 gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga
    12241 tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat
    12301 aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg
    12361 gtacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt gccatttcaa
    12421 cgtaatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct
    12481 cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg
    12541 ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg cggcgtacgg
    12601 atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg aaacagaatg
    12661 ggcaatgcat acggcgtcct gaccaggtgg ttaacggaaa tctattttac cctgaactga
    12721 cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc tgcttttacc
    12781 ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta acgtttttcg
    12841 actgctcctg ctgggaagcc ttagcgcctg tgtgcagcaa agtgaagtgc gacagatgaa
    12901 acacagcgtc agcacgctga accaggagat gacgcaactc aatcaagaaa cagtcaaaat
    12961 cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc tgcctggagc
    13021 gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc tggtgaatat
    13081 tacgcctgat acagatggca ccacccttac actacgtatt cagggtgagt cgaatgatcc
    13141 cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa tagacaactt
    13201 tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc tcgcccccag
    13261 cgatgttgat attccgttac aattaaaggg gatttctgtg gatcagttag gctttgtacg
    13321 tatccacgac attcaacctg ttatgcagta aacgtatttc gggacgattt tgtgcgtccc
    13381 gcaacatctt ttcccgtcat tttgttactc tgcttacatc acctggattg atagtaaaag
    13441 tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa tgtgtgtaaa cgtgaacgca
    13501 atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc aatgattttt
    13561 ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt tttcactccc
    13621 gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca gccggtgcgg
    13681 tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa ccgcagtatc
    13741 tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc cgtttcggac
    13801 cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa gactggttcg
    13861 acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat gctaaaggta
    13921 tctggaacca cggttctccg ctgtttatgg aaatcgaacc acgtttctcc atcgacaagc
    13981 tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg aacaactaca
    14041 tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg ggtctgggta
    14101 ccgatatcga cactggcctg ccgatgagcc tgtccatgaa cgtctatgcg aaataccagt
    14161 ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc aaaattaaat
    14221 actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc ttcaccaact
    14281 tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt attaagaccc
    14341 gtactaataa ctctatcgct tccagccata ttctggctct gaactacgat cactggcact
    14401 actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat gcagaactga
    14461 acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac ctggtagtag
    14521 gttacaactt ctgattatga aaatgccggg atttattccc ggcatttctg attgttaaca
    14581 acgtgtgctt tgttcatgcc ggatgcggcg taaacgcttt gtccggccca caaaagcacg
    14641 taaattcaat atattgctga aataacgtag gcctgataag cgtagcgcat caggcaattt
    14701 tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat gggttgtact
    14761 tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta ccatactgat
    14821 ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacacaac aattcccagt
    14881 tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaacg gtaagttcac
    14941 gcaggttaga ataaacaaac tcgcgggaac agcgaccgca gacccacgga tagattttta
    15001 atgctttttc gcgatagccg ctttctaacc gtgcatagtt ttttgggatg atagccatga
    15061 aaagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg ggattttcgc
    15121 aatctccata catcaaaaaa aatcccgatc ttctgaccgg gattttcaac atcaacggga
    15181 acttgattaa ggcagaattg acggctgatc cgccccttct ttttccactt tctgctgcaa
    15241 catgtgttcg cgcttcatac ccagtttcag agccaacgca gatgctacgt agatggaaga
    15301 tgcagtgccg atggaaacac cgataagcat ggtcagcgag aagccttcca gtaccggacc
    15361 accgaagaga tacagcatca ggataaccat caaggtagta ccggatgtga tcaaggtacg
    15421 gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt
    15481 gcagaagttt tcacgaattc ggtccgatac cacgatactg tcgttaagcg agtaaccgat
    15541 aaccgacatc aacgatgcca caatggtcag gtcaatctcg atatggaata acgacaaaat
    15601 acccagcgta atgataacgt cgtgcgccag cgcaataacc acccctgccg ccagtcgcca
    15661 ctcaaagcgg aaacctacgt acacgaggat agacagcagc gctgccatca acgccatcgc
    15721 accggtttgc gcaaggtctg cccccacgct cggaccgacg aactcaatac gcttcactgc
    15781 tgcattctga ttggtggatt cgttaatcac cttcagaact tggctgccca gcacctgacc
    15841 gccggtttcg ccttcagcag gcggcatacg gaccatgatg tcgtggctgc taccaaagtt
    15901 ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc
    15961 agccggtttt tcgagcgtaa tttcgataac cgtaccaccg gtgaaatcca gcccccagtt
    16021 aaagccgcgc acgcccataa taacgatagc agcgattaac agcagaccag agatgccgaa
    16081 agcccagtag tcccagcgca taaagtcata gactttacgg ccgtggttta gttgttcaac
    16141 agtatattcc tgtgccacat cgcactcctc agattgacag cttcttgacg cgcttgccgc
    16201 catatagcag gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca
    16261 caccgatacc ggtagtaatc gcgaaccctt taattgcccc ggtacccact gcgtacagga
    16321 tgatgacttt aatcagcgtg gtgatgttcg catcgaagat agaactgaat gcgccacgat
    16381 aaccttcatc aattgcctgt tgaacagtac gtccgttgct caactcttct ttaatacgtt
    16441 cgttgatcag tacgttcgca tcgaccgcta ccgcaagggt taagacgata cccgcgatac
    16501 ctggcatact cagtgttgcg cctggcaaca gcgacataat accgacgatc aagatcaagt
    16561 tggcaatcag agcactggtc gcaatcagac caaacttctt atagaagatg atcatgaaca
    16621 gaatagaaac cagcagaccg gcaaggcaag cctccagccc ctgctcaatg ttctgcatac
    16681 ccagggtcgg gccaattgta cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg
    16741 cacgcagcag cagtgacagc tgacgggctt cgttcgggtt gttgatgccg gtgatacgga
    16801 agctgttacc cagacgagac tggatgttgg cgatgttaat cacctcttcc tgtttcacca
    16861 gaaccgcacg accatttgca tctttcttac cgctgtcttt gtactccaca aacagggttg
    16921 ccatcggttt gccgatgttg tccttggtga agttagacat aatgttgcca ccagcgctat
    16981 cgagcgagat gttaacctgc ggctggttgt attcgtcctg gctggaagtg gagtcggtga
    17041 tatggtcacc agtcagaatt acgcgtttgt acagcacaac cggctgacct tcgcgggtct
    17101 gtttcacttc agagtcgccc ggtacgcgac cggatgccgc agcggcctga tcaacattgg
    17161 tgtttaccag acggaattcc agcgttgccg tcgcgcccag aatctctttc gcacgcgcag
    17221 tgtcctgaat accaggcagt tcaacaacga tacggtcagc accctgacgc tgaaccaccg
    17281 gctccgccac gccaagttgg tttacacggt tacgcaggat attaatgttc tgctgcaccg
    17341 catattcacg cgcttcactc agacgagcat cgctcattac cgcacgcagc tggttgctgc
    17401 cctggctgct aatcaccagg tccggatggc gcttgctcag atacgcaatg gcttcatcac
    17461 gagctttagc atcgcggaaa gtgatgctca ggccgtagtt gttttcttta cgaacagtgg
    17521 tatacgggat gcccttttcg cgcaggtcac tgcgtaggct atcgatattt tgttcctgga
    17581 gtttgccaag cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg ccacgcaggt
    17641 caaggccgag cttcatcggc tcagcatgaa tagctgccag ccagcgcggc gttgccgggg
    17701 caaggttaag cgccacgacg tatttgtcac ccataacgcc cattaatgct tcacgagcgc
    17761 gcaactgggt gtcagtggag tcgaagcgcg caagaatagc gccctcttcc agtgccacag
    17821 acttagcagt tattttttct tcttgtaacg ttttctggac ctggatcagc gtttgctcac
    17881 tggcggcgac tccgcgcgca ccagtgatct gaacagccgg atcctcacca aacaggttgg
    17941 gaagcgcata cagcagaccg atgacaatca ccacgatcag catgacgtac ttccacaaag
    18001 gataacggtt taacacggca attcccttag ggaaaaattt taattacagc gccttcatgg
    18061 tgcctttcgg caggacggca gctacgaagt cacgtttaat aactacttca gtggtgtcat
    18121 tcagcgcgat agcaatgtag ccgttttccg ctactttggt tacgcgacca accaggccac
    18181 cgttcgtcag aacttcatca cctttggcaa tggagtccat cagctttttg tgttctttgg
    18241 tgcgcttctg ctgtggacgc aggatcatga aatagaaaat cagaccgaat accaccagca
    18301 tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc cgttgccgct accgcatcag
    18361 aaataaaaaa gctcattaaa tttccctcat tattaatatt aatcaacgtt caaaggtggt
    18421 acttctcgcc cctgacgctg gtaaaaatca gttacgaagc tctctaattt accctcttca
    18481 atagccttgc gtaaacccgc catcaaacgc tggtagtaac gaaggttatg aatggtgttg
    18541 agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat gcaagtaagc gcgtgaataa
    18601 ttgcgacagg tgtagcaatc acactcagga tcgagtgggc cagtatcgct cttatatttc
    18661 gcattgcgga ttttcaccac gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg
    18721 gttggcatta cgcagtcaaa catatcgata ccgcgacgta cgccttcaac caggtcttcc
    18781 ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg acacacatgc
    18841 tccagaatac ggtgcatatc tgctttcggc tcacccacag ccagaccgcc gacagcgtag
    18901 ccatcaaagc cgatatctac cagaccttta acagatatat cacgtaaatc ttcgtaaata
    18961 ctgccctgaa ttataccaaa taacgcattt ttgttaccaa gactgtcaaa acgctcacgg
    19021 ctacgcttcg cccaacgcag agacatctcc atagagcgtt ttgcgtaatc ccagtcagca
    19081 ggatacggcg tacactcatc aaagatcatg acgatatccg aaccaagatc gtactgaatc
    19141 tccatcgact tttccgggtc gaggaaaatc gggtcgccgt tgatcgggtt acggaagtga
    19201 acgccctgtt cggtgatttt acgaatatca ccaaggctga agacctggaa gccgccggag
    19261 tcggtaagaa tcggtccttt ccactgcata aaatcgtgca gatcgccgtg cagtttcatg
    19321 atttcctggc ccgggcgcag ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca
    19381 gtggcttcaa cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc aacaggcata
    19441 aaacaaggcg tttccactac gccacgatca aagaccaggc ggccacggcg tgcgcgaccg
    19501 tcggtggtgt ccagttcaaa tttcattttt tctccactac gtcagaaaaa cagtccaacg
    19561 tttaaaccag cgccgcggaa ttactccccg acgcgctcat taattgcctg cggattgtac
    19621 gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt tctcttctac cgccgcttta
    19681 taggcattca tggtgtgttg ataaccggca aaggccgaaa ccagcataat cagcgtcgac
    19741 tctggcaagt ggaagttcgt caccagcgca tcgaccactt tgtactggaa gcccggatag
    19801 ataaagattt gggtatcgtc aaagaacggt tcgataagat cgttttttgc cgcctgtgcc
    19861 gagctttcca gcgaacgtac tgaagtggtg ccaaccgcaa tcacccggtt accgcgcgct
    19921 ttcgccgcca gtaccgcgtc taccacatcc tgcggtactt cagcgtattc cgagtgcatg
    19981 atgtgatctt caatggtgtc gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac
    20041 gtcacaaacg ccatctccac gcctttggcg cgtaattttt ccagcaaagg ctcgtcaaaa
    20101 tgcagaccag cggtcggggc tgcaaccgcg cccggttttt cgctataaac ggtttgataa
    20161 agttcgcggt cagcgtcttc gtccggacga tcgatatacg gcggcagcgg catatggccg
    20221 atgctgttga ggatatccag taccgagcgt tcatcattaa attcgacttc aaacagtgcg
    20281 ccgtggcgcg cggtcattgt tgcgttgata ctttcgtcat cgcccagcaa cagttctgcg
    20341 ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc
    20401 cgctcaacca gcacttcaat cttgccgccg ctggctttac gcccaaacag acgcgccggg
    20461 atcacgcggg tattattaaa aaccagaaga tcgccggggt tgagcttatc gagtaaatcg
    20521 gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta
    20581 ctgcgttcag gcatgggata gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg
    20641 gtaacgcgca tgacactgac tcagactaaa ataagagtcg ggtagtctag tgccggggcg
    20701 ctctccctgc aacctttacc cccctttgga tagaatataa tcgatgaatt ttttagctca
    20761 cctgcattta gcccatctcg cggaaagctc gctttctggc aatttactgg ctgatttcgt
    20821 acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg gctggcattc atatgcatcg
    20881 acgtatcgac gtattgactg acaatctgcc ggaagtccgc gaagcacggg agtggtttcg
    20941 tagtgaaacg cgccgcgttg cgcctattac gctggatgtc atgtgggatc actttctttc
    21001 ccgccactgg tcgcagctgt cgccggactt tccgctacag gaatttgtct gttatgcccg
    21061 cgagcaagtg atgacgattt tgccggactc accgccacgt tttatcaatc tgaacaatta
    21121 cttgtggtca gagcagtggc tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt
    21181 aaacggcatg gcaagccgcc gcccacgtct ggatgccctg cgtgactcct ggtacgattt
    21241 agacgctcat tatgacgccc tcgaaacccg cttctggcag ttttatccgc ggatgatggc
    21301 gcaggcgtca cacaaggcgt tataaaacgt tagttcatcc ataccgtagc cgaaatggca
    21361 ggcaaagcca gaatcccgtc agtcagttgc ccatgccctt ctttgcattg ccattgcacg
    21421 gcattgagaa acggtgacgc gggtagcacc acttcacagg cctcgccacg gttgattgcc
    21481 accagtacac gttgctgatt cagcacgcgg acaaatacca ctacgttatc ttccgcatac
    21541 agcacctgac agccgccacg acgcagcgcc tgacttttct tacgcagcac aatcattcgc
    21601 cggtacagcg cgaataacgc cgtatcctgc ttttccacct gccaggggaa cggtttacgg
    21661 caaaacggat cgtttttacc atccagtccc acttcatcac cgtaataaat gcacggtaca
    21721 ccaggccagg tgaacagcca gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc
    21781 agcgttttaa atcgcgcagt atcgtggcta tcgagctgat taaacatacg taattgttgt
    21841 tgatgagaaa gccctgcgcg gtaattatcc atccaggcca tacaggtttg ggcgtcaatt
    21901 tgctgcggat cataagagat gtcggtattg gcaagaaatc cccacaacgg gaatgtgaag
    21961 ccacgatagt tcatggcagc atcttccaca tcggcctgta accattgccg tgcatcgcca
    22021 aaatgttcgc cgacaatata cgcgtccggc tgggtttctt tcgccgcttc ggtgatcccg
    22081 gcaacgtgct gcatattatt gcgcgcccca cccgcctccc ccagcatatg caccacatcc
    22141 agccgccagc cgtccatatt ccacggcgct ttcagccagt ggcggacaat actgtcttcc
    22201 ccgcgataaa tttcattcac cagactttcc gactgataat ccagcttcgg caagctggca
    22261 tagccaagcc agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc
    22321 gattcagagt tgtgacaagc tccccccgtg ccaagattgt gcctgtcaaa ccaggcatgg
    22381 gaatcgccac tgtggttaaa cacgccgtcc agcaccagcc gcattcccag ctgctgcgta
    22441 ttgtgtcgca aacgcagcaa caccccatca ccgccaaact gcggatcgac atggcgataa
    22501 tcctcggtat cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgct
    22561 gtcacgccaa gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca
    22621 tagaacgttg atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc
    22681 tcttgtccgg ctgcatgatg gtaatagaca tgatcctgtt cagcttcacg aggaagacta
    22741 cgcgcaaaac gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg
    22801 atatccggta catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc
    22861 tgcggtgtaa accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg
    22921 ggttgtccgc tggagagatc aatcgccgca cgccatgcgg tgacgcctgg ctgcggctga
    22981 ctgcgctgct tatgcattgg tactgacatt tcttcgttat cgtgttctgt acgcagcata
    23041 atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt
    23101 tgtttaacaa atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga
    23161 tgaaaatctg atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt
    23221 tgggggagga ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga
    23281 accggcttac agaacgcaag tattattggt tttcagccag ctgacgatcg tggcggcgtt
    23341 taaacatcca gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac
    23401 gcgtatccgg gtgataacca atcaacccga taataaagag caggaaaatc agcccgccga
    23461 tggtcgttgc taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca
    23521 aacggcgacg gaaggcgatt tgcgacagca ggatcataat ccacacccat accgtggcga
    23581 aggttgccag cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca
    23641 ccgcaaacag cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg
    23701 acgttttgct gaaaattttc ggtgcgctgc cctgctctgc cataccgtgg agcatacggc
    23761 ctacgccaaa tacgtcactg ttaattgccg acagcgaagc ggtcagcaca acaaagttaa
    23821 gaatgctggc ggcaaaggta atgcccatat gctggaacgt cagcacgaac gggctaccgg
    23881 cagtgccaac ctgattccac gggtagatag acataatgac gaacagcgta ccgacgtaga
    23941 acaccagaat acgcatcggc acggagttaa tcgcacgcgg aatcgatttc tcaggatctt
    24001 tcgcttcacc ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt
    24061 gcaacgacat taccatgcca agccagccgt tactgaagaa gccgccgttg ctccacaggt
    24121 tatgaatacc ggtcggttgc ccgccgttgc caacccccca gatgatgatg ccgaaaccgg
    24181 cgacaatcat gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctcac
    24241 cgaagacctt cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc
    24301 aaatccagtg cggcaccgtc gggaacaaga cacccatata gataccaaaa gcggtcacat
    24361 cggcgatggc gacaataagg atttcaaagc agtaggtcca tccggtaatg taacctgcca
    24421 gcgggccaag gttttcctgc gcataacgcg agaaagagct ggcggccggg ttatgtaccg
    24481 acatttcccc cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca
    24541 acaacacgct cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtgc
    24601 caattgctga acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct
    24661 tattcttact ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc
    24721 agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac
    24781 ctgccgcgcg atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg
    24841 ccagaccttg ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg
    24901 cagatgcttt gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa
    24961 tcacgcggga cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac
    25021 acggcggata aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga
    25081 ggttagacac caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg
    25141 gtacgtactg ggcaaagaat tctgcacaag cacaggtcag gccaaccgcc gtgaccagac
    25201 aggcgatgaa gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa
    25261 cgtaagcatg cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg
    25321 aacccagaca gaacagcgcc aggtacagca aagtcagacc aacacccgcc atcaggccag
    25381 cccagacggt ataacgggtc agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt
    25441 taacaataac gataccaaac accattgcgc ccagcgtatc catggtcaga tagccattaa
    25501 cgaagccgtt agaaaacgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg
    25561 ccggccagac aatcgcggca acagacagga tgaccagcgc gataattttc agcggcgcaa
    25621 ggaagttgcc cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag
    25681 cgaaatagac caggctgtag ataaacagcg gcagcgcgga atcacccgtc agcggcgcaa
    25741 tccccacttc aaaggaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca
    25801 ggtaacaaac cgttgccagc agtacgccag cgactttacc gattggcgtg ctgaggctgt
    25861 caacaccgcc gccaactttt gccagcgcca ctaccgttaa taccggcagg ccaacggcag
    25921 taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc tgcctgtaag ccgaccattg
    25981 gagggaaaat aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga
    26041 tgatatcgcg cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg
    26101 taacgttaaa tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt
    26161 agcgggaaat actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat
    26221 gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg
    26281 agggatctaa aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta
    26341 tttccgtgaa aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc
    26401 taacgcccgt attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa
    26461 aagatgacaa aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa
    26521 ctgaaacgtg ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc
    26581 accgcatgtt tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct
    26641 ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt
    26701 ccgttatctt caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata
    26761 tgcgtgcctt ccggcgtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt
    26821 agctgatctt cgttgccaga caccttgagg ccgttatcta tctcaaaggt aaatgtctgt
    26881 tttttctgac tcagagtctg agcctcgcgc tcaacaacgc gcagcatcat cggcacatca
    26941 accttttcat tgagcaattg cgtcggcgcg gcttcaattt tcgacagcgt tagcaactgc
    27001 ttcaccagcc cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt
    27061 accgcgcctt ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat
    27121 ggcgtacgta actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga
    27181 tgcatttgcg tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg
    27241 cgaatttcca gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc
    27301 gttttcagat attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta
    27361 tcttccggcc agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg
    27421 ccctcttccg tagtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt
    27481 ttaatcagat tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag
    27541 ccgtatagta gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc
    27601 cacagccacc atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc
    27661 gatgccagca aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg
    27721 cagcaaagta gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat
    27781 actccagtta agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg
    27841 gttgaaaaac gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc
    27901 tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg
    27961 ttagttcccc agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc
    28021 ataaaaaagt gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc
    28081 atcactcggt gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc
    28141 gccattggcg aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa
    28201 aatggcttgg tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct
    28261 tcccctctgg cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg
    28321 tgtttgatga actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc
    28381 ggccagggtt cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga
    28441 aagccatttt gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg
    28501 accagaatac gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg
    28561 cgacgtcatt atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc
    28621 aggttattga tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg
    28681 cgaccatgct ttttcgctag ggaaccgtta tgcgcatcct tcacacctca gactggcatc
    28741 tcggccagaa cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc
    28801 tgctggagac agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg
    28861 ataccggctc gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac
    28921 agcaaactgg ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga
    28981 atgaatcgcg cgatatcatg gcgttcctca atacaaccgt ggtcgccagc gccggacatg
    29041 cgccgcaaat cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt
    29101 ttttacgccc gcgtgacatt atttccagcc aggcggggct taacggtatt gaaaaacagc
    29161 agcatttact ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac
    29221 tgcgcggcga tcagcctctg cccatcatcg ccacgggaca tttaacgacc gtgggtgcca
    29281 gtaaaagtga cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccggcacaaa
    29341 actttccacc agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg
    29401 gcatggaaca tgttcgctat tgcggttccc ccattccact gagttttgat gaatgcggta
    29461 agagtaaata tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc
    29521 tgaacgtacc ggtaactcaa cccatggcgg tgctgaaagg cgatctggcg tcgattaccg
    29581 cacagctgga acagtggcgc gatgtatcgc aggagccacc tgtctggctg gatatcgaaa
    29641 tcactactga tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat
    29701 tgcctgtcga agtattgctg gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca
    29761 gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg
    29821 cactggaaga actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt
    29881 tgcataccct cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct
    29941 gaactcatta aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg
    30001 gctgtttgcc attaccggcc caaccggtgc ggggaaaacc accctgctgg acgccatttg
    30061 tctggcgctg tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct
    30121 catgacccgc gataccgctg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc
    30181 gtatcgcgcg ttctggagcc agaatcgggc gcgtaaccag ccggacggta atttgcaggt
    30241 gcctcgcgta gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga
    30301 taagctggaa ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat
    30361 gctgctttcg caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga
    30421 attgctcgag gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga
    30481 gcagcacaaa tcggcccgca ctgagctgga gaagctgcaa gcgcaggcca gcggcgtcac
    30541 gttgctcacg ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga
    30601 agaaaaacag ttaattaccg cgcagcagca agaacaacaa tcgctaaact ggttaacgcg
    30661 tcaggacgaa ttgcagcaag aagccagccg ccgtcagcag gccttgcaac aggcgttagc
    30721 cgaagaagaa aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa
    30781 tcttcgtcca cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca
    30841 gcagattgaa gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg
    30901 ccaccacgcg gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg
    30961 gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca
    31021 gttctcccaa caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca
    31081 tgctgagcaa aaacttaatg cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt
    31141 tgctaccgcc ctggcgcaac atgccgagca acgcccactg cgtcagcgcc tggtcgcgct
    31201 gcatgggcag attgttcccc aacaaaaacg tctggcgcag ttaatggtca ctatccagaa
    31261 tgtcactctg gaacagacgc aacgtaatgt cgcacttaac gaaatgcgcc agcgttataa
    31321 agaaaagacg cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa
    31381 aacgctggaa gcccagcgtg cacagttaca ggcgggtcag ccttgtccac tttgtggttc
    31441 caccagccac ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg
    31501 attactggcg ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg
    31561 gcaactggat gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg
    31621 acaagatgag caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac
    31681 cttgcagcca caggacgata ttcaaccgtg gctggatgca caagatgagc acgaacgcca
    31741 gctgcggtta ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca
    31801 aattatccag tatcaacagc aaattgaaca acgtcagcag cagctcttaa cggcattggc
    31861 gggttatgca ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca
    31921 gcaagaagcg cagagctggc agcaacgcca gaacgaatta accacgctgc aaaaccgtat
    31981 tcagcagctg acgccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga
    32041 agaaactgtg gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag
    32101 ccagcagcag acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca
    32161 agcgcagttt gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc
    32221 ggcgctaatg gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa
    32281 ccagcgccgt caggcgcaaa cgctggtcac tcagacagca gaaacgctgg cacagcatca
    32341 acaacaccga cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt
    32401 agcgcaaact caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca
    32461 gctgaagcag gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca
    32521 aatgacgcag caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg
    32581 cgataaattc cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa
    32641 tcagcaactt acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct
    32701 ggaagtcgag gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc
    32761 cggcggcgaa agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag
    32821 ccataaaaca cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga
    32881 aacgctggat accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg
    32941 tgtgattagc cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa
    33001 gatcaacggc ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag
    33061 caggataatg aatacagagg ggcgaattat ctcttagcct tgctggtcgt tatcctgcaa
    33121 gctatcactt tattggctac ggtgattggt agccgttctg gtggttgtga tggtggtatg
    33181 aaaaaagtca ttttatcttt ggctctgggc acgtttggtt tggtgatggc cgaatttggc
    33241 attatgggcg tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg
    33301 catatgatct cgtattatgc actgggggtg gtggtcggtg cgccaatcat cgcactcttt
    33361 tccagccgct actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc
    33421 aacgccatgt tcacgctctc ttcgtcttac ctgatgctcg ccattggtcg gctggtatcc
    33481 ggctttccgc atggcgcatt ttttggcgtc ggagcgatcg tgttatcaaa aattatcaaa
    33541 cccggaaaag tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg
    33601 ctgggcattc cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta
    33661 ttgatcgctg tttttaatat tgcggtgatg gcatcggtct atttttgggt gccagatatt
    33721 cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact ttttgcgcag cccggccccg
    33781 tggttaattt tcgccgccac gatgtttggc aacgcaggtg tgtttgcctg gttctgctac
    33841 gtaaagccat acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg
    33901 atgttagt
//
LOCUS       DADKXM010000029        33663 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.029, whole genome shotgun sequence.
ACCESSION   DADKXM010000029 DADKXM010000000
VERSION     DADKXM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.029"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1327..2106
                     /gene="ipaJ"
                     /locus_tag="JQR05_001381"
     CDS             1327..2106
                     /gene="ipaJ"
                     /locus_tag="JQR05_001381"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY5323584.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3053..3982)
                     /locus_tag="JQR05_001382"
     CDS             complement(3053..3982)
                     /locus_tag="JQR05_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY5323585.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4199..4435)
                     /locus_tag="JQR05_001383"
     CDS             complement(4199..4435)
                     /locus_tag="JQR05_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY5323586.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4439..6340)
                     /gene="ipaA"
                     /locus_tag="JQR05_001384"
     CDS             complement(4439..6340)
                     /gene="ipaA"
                     /locus_tag="JQR05_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY5323587.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSLGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQPDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6349..7347)
                     /gene="ipaD"
                     /locus_tag="JQR05_001385"
     CDS             complement(6349..7347)
                     /gene="ipaD"
                     /locus_tag="JQR05_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY5323588.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDTLSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKNALEELKKKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7398..8489)
                     /gene="ipaC"
                     /locus_tag="JQR05_001386"
     CDS             complement(7398..8489)
                     /gene="ipaC"
                     /locus_tag="JQR05_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY5323589.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIAHLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSKNSTASQIAGNIRA"
     gene            complement(8509..10251)
                     /gene="ipaB"
                     /locus_tag="JQR05_001387"
     CDS             complement(8509..10251)
                     /gene="ipaB"
                     /locus_tag="JQR05_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY5323590.1"
                     /translation="MHNVSTTTTGLSLDKILTSTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KSFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10257..10724)
                     /gene="ipgC"
                     /locus_tag="JQR05_001388"
     CDS             complement(10257..10724)
                     /gene="ipgC"
                     /locus_tag="JQR05_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY5323591.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10782..11408)
                     /gene="ipgB"
                     /locus_tag="JQR05_001389"
     CDS             complement(10782..11408)
                     /gene="ipgB"
                     /locus_tag="JQR05_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY5323592.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11424..11813)
                     /gene="ipgA"
                     /locus_tag="JQR05_001390"
     CDS             complement(11424..11813)
                     /gene="ipgA"
                     /locus_tag="JQR05_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY5323593.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11826..13310)
                     /gene="icsB"
                     /locus_tag="JQR05_001391"
     CDS             complement(11826..13310)
                     /gene="icsB"
                     /locus_tag="JQR05_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY5323594.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPMLVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13624..15240
                     /locus_tag="JQR05_001392"
     CDS             13624..15240
                     /locus_tag="JQR05_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY5323595.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNPKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTKYLNNKNWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESQHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRRELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVNVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKDIVNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHDTGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15250..15612
                     /gene="ipgE"
                     /locus_tag="JQR05_001393"
     CDS             15250..15612
                     /gene="ipgE"
                     /locus_tag="JQR05_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY5323596.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15612..16070
                     /gene="ipgF"
                     /locus_tag="JQR05_001394"
     CDS             15612..16070
                     /gene="ipgF"
                     /locus_tag="JQR05_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY5323597.1"
                     /translation="MSRFVFILLFFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESR
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16079..17194
                     /gene="mxiG"
                     /locus_tag="JQR05_001395"
     CDS             16079..17194
                     /gene="mxiG"
                     /locus_tag="JQR05_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY5323598.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICEANKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17202..17453
                     /gene="mxiH"
                     /locus_tag="JQR05_001396"
     CDS             17202..17453
                     /gene="mxiH"
                     /locus_tag="JQR05_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY5323599.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGELTLALDALARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17466..17759
                     /gene="mxiI"
                     /locus_tag="JQR05_001397"
     CDS             17466..17759
                     /gene="mxiI"
                     /locus_tag="JQR05_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY5323600.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVGNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17765..18490
                     /locus_tag="JQR05_001398"
     CDS             17765..18490
                     /locus_tag="JQR05_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY5323601.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSEVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVTLLVWAFKTGWFKRNKI"
     gene            18487..19014
                     /locus_tag="JQR05_001399"
     CDS             18487..19014
                     /locus_tag="JQR05_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY5323602.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18956..19651
                     /locus_tag="JQR05_001400"
     CDS             18956..19651
                     /locus_tag="JQR05_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY5323603.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIINTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19644..20051
                     /locus_tag="JQR05_001401"
     CDS             19644..20051
                     /locus_tag="JQR05_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY5323604.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFDAGREADDSTW"
     gene            20035..20463
                     /gene="mxiM"
                     /locus_tag="JQR05_001402"
     CDS             20035..20463
                     /gene="mxiM"
                     /locus_tag="JQR05_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY5323605.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20593..21225
                     /gene="mxiE"
                     /locus_tag="JQR05_001403"
     CDS             20593..21225
                     /gene="mxiE"
                     /locus_tag="JQR05_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY5323606.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21212..22912
                     /gene="mxiD"
                     /locus_tag="JQR05_001404"
     CDS             21212..22912
                     /gene="mxiD"
                     /locus_tag="JQR05_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY5323607.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22931..23998
                     /gene="mxiC"
                     /locus_tag="JQR05_001405"
     CDS             22931..23998
                     /gene="mxiC"
                     /locus_tag="JQR05_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY5323608.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24011..26071
                     /gene="mxiA"
                     /locus_tag="JQR05_001406"
                     /gene_synonym="virH"
     CDS             24011..26071
                     /gene="mxiA"
                     /locus_tag="JQR05_001406"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY5323609.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26084..26485
                     /gene="spaK"
                     /locus_tag="JQR05_001407"
     CDS             26084..26485
                     /gene="spaK"
                     /locus_tag="JQR05_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY5323610.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIIFAEILHEFYQRMEILNEVL"
     gene            26489..27781
                     /locus_tag="JQR05_001408"
     CDS             26489..27781
                     /locus_tag="JQR05_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY5323611.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27874..28212
                     /gene="spaM"
                     /locus_tag="JQR05_001409"
     CDS             27874..28212
                     /gene="spaM"
                     /locus_tag="JQR05_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY5323612.1"
                     /translation="MEALDKRIIYFQQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28199..29077
                     /gene="spa32"
                     /locus_tag="JQR05_001410"
     CDS             28199..29077
                     /gene="spa32"
                     /locus_tag="JQR05_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY5323613.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29071..29952
                     /gene="spaO"
                     /locus_tag="JQR05_001411"
     CDS             29071..29952
                     /gene="spaO"
                     /locus_tag="JQR05_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY5323614.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29957..30607
                     /gene="spaP"
                     /locus_tag="JQR05_001412"
     CDS             29957..30607
                     /gene="spaP"
                     /locus_tag="JQR05_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY5323615.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30622..30882
                     /gene="spaQ"
                     /locus_tag="JQR05_001413"
     CDS             30622..30882
                     /gene="spaQ"
                     /locus_tag="JQR05_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY5323616.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30882..31652
                     /gene="spaR"
                     /locus_tag="JQR05_001414"
     CDS             30882..31652
                     /gene="spaR"
                     /locus_tag="JQR05_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY5323617.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31659..32687
                     /gene="spaS"
                     /locus_tag="JQR05_001415"
     CDS             31659..32687
                     /gene="spaS"
                     /locus_tag="JQR05_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY5323618.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32700..32942
                     /locus_tag="JQR05_001416"
     CDS             32700..32942
                     /locus_tag="JQR05_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323619.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33519..>33663)
                     /locus_tag="JQR05_001417"
     CDS             complement(33519..>33663)
                     /locus_tag="JQR05_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5323620.1"
                     /translation="LKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFKSRDISSAYG
                     KTD"
BASE COUNT        10498 a         4900 c         6742 g        11523 t
ORIGIN      
        1 ttaccccctg gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca
       61 cttgttgact gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc
      121 atgcggaacg ttggaaccta tatccccccg ataccaaagg cgaaatgtaa gtgacaaaat
      181 gcaatgaaca cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa
      241 ggtgtagagt ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac
      301 agattgtaga atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg
      361 tcattcaggt tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct
      421 gtcagagttt tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt
      481 atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga
      541 tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca
      601 ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga
      661 agaactgcag gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag
      721 cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat
      781 atcattactg cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac
      841 ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct
      901 tctgagtatt aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc
      961 ggcagcctta ctgatcaagg agttggggga gttgataaca aagttgaaaa aacagctctc
     1021 aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc
     1081 gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc
     1141 tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg
     1201 tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac
     1261 ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac
     1321 tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg
     1381 ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact
     1441 gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag
     1501 atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt
     1561 gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat
     1621 ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga
     1681 atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct
     1741 acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta
     1801 attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta
     1861 catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt
     1921 attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat
     1981 ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt
     2041 aattctaact ttataggata tactaaaata ggtatttcca tagttataac taatgaggct
     2101 ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggcg ggggggcata
     2161 acaaatgata ttactgagta caaatttagc tatattgggc ggattagttg tgtgggaaga
     2221 cgaaatgttt ctcttgaaat aagtgaactc cctcaaggaa tgattgtgtg cttaaatatt
     2281 taagtgctgg taagtttgag aaggctgaca tattttgcag agcaaacagt gattgacttg
     2341 gatgatattt ttattttagt cactcgttgt aattggagta tgctttattt tatttgaaaa
     2401 tagatgtatt ttatacccgc aatttaaact gtaagcccta tgaggggtat ggatattata
     2461 agttactctt tgactaagca taacttttgg ggttagaaaa ataaatctct tgttaataat
     2521 taatatgatt ttaaaatcaa acttgtttat aattataaaa taatgtaagt cggtcatttt
     2581 atgggaagat taggagaata aaacagtaat tatatttgag aaatctgcta atatattctt
     2641 aaagcttttt tgatactgtg aacgaagtct gttaatggtg gattttttgt ttatcaccat
     2701 gtaaaaaaat tgagtagtaa aagcgttgac tgctaatcct cgctgttttt attgaaacga
     2761 ttaaaaatac gctgaatatt ttcggccagt cactcatctt cacataaaat tttgaccatc
     2821 atgccgcatc ccttagttgc acaattcatt ttcaatgata tgaatattac taatttcacc
     2881 agataatgtt ctcgtggtca gcagggtatg agttttcacc gtaattttaa ggaaacacag
     2941 tgcgcgggga ttgaataaca gatagccgac ggaattaccc caccggctga aagtcaagtt
     3001 caacgaagat ttttattatc tgaattgggc agtttacatc agtgttcgat gtttatgaag
     3061 acgatagatg gcgagaaatt atatcccgaa tagcttcatc tatttctctc tgaactgttt
     3121 tgtttatttt agataatgta aatattagag ttctgtttgt tttattctct tttcttttta
     3181 tgtaagtccg tttgtcttta gaaataaata acgagtcaac ttttagcgaa gggttttgct
     3241 ttctgttttt gcttttcttt atgatgttta aaatttcttt tttatatatg tccgggggca
     3301 aatttgtatc aaggtctttt atttcttcct ttaatattgg tagtgtagaa ctaagagatt
     3361 cattagcctt ttctagtcct ttataataat tgaataatat cttgtagtca ttaaagttaa
     3421 gttctgacgc gattggaaat agagatatta tttcctgtgg aacgcttgct gcctgaaagg
     3481 cacgagtgac tttcgcgcga gacagattct cttttttggc tatgtcttta taggacatcc
     3541 ctgatacttt cagaaaatta agaccaatac caagttctcg gatgctatgc tcttttgctg
     3601 tttgtatatc gtttgctagt tttctggcat caagagtaga tatatattct tttgaatata
     3661 gaacttcaag atctgctcct gcatatattg cagatgctct tctacgagtg ccatccagaa
     3721 tttcaattct accatcaatc tcccttccta ttacagggaa gaattgttgt agctttatag
     3781 tttttatgat atcggctaat gattcttctg ttagggattc ttgatcgcgt acattcgttt
     3841 tttggttgac gaaggttaaa tcttggatcc ttttatgagg aatcttggct ttgataaagg
     3901 aaactttatt accagaatgg agtgtaaact ctttcttttg gccttccttt atacttaaca
     3961 agtcgttgca caaatccacc atatcacacc ctgtttattc atattgattt atattgacaa
     4021 aagttaaatg cagtgtgagc aagattgtat gtaactgttg atgatagtca acggtagtct
     4081 taaaaattac tattttaata gctggattga tgcaatttat ctttattgga taaaagaata
     4141 atattcttgt ggagctctga tgtgagagct ccatagatga aaaaatgctt atcaattact
     4201 atattgattt tgtactattt ttgactacag aaataagatc tccaatagta ataatgtgtt
     4261 ctaatgcaga ttcatctata cgtagattga actcaaaact aatcatatcc agcatttcaa
     4321 tcagagaaag agaatcggca gaaagatctt caataaattt agtttcttca gatagtttgc
     4381 tgtacgctat accatagcaa aaggccacta ttgataatat tttttctttt atcatatttt
     4441 aatccttatt gatattcttt aatacttttg atagggaagt ggttacatct tttgctgctt
     4501 tatatatatt attgttttca gctgtaatat cattgttatt ttttaaatca gatatatcat
     4561 ctgtaagtaa ttctgcagag gtatcgtcaa tttttgacaa taccttgctg agggcgctag
     4621 atacttcttt tgccttttca tagatcgcat gatttttatc aattgtatct gcgttggttt
     4681 ctgttacgta gtttattgta tttgcatctg taacttttaa tttgttgttt attctgtgtt
     4741 ctgttgtgga ttttgtaatg tcctttgact tttcctgtaa ctctcgttct tgtgttcctg
     4801 atttagttcc aatgagagat ataagattac ttagggagtt tgttactttt tttgaagctt
     4861 caaatatcgt ttcccgtgtt aaatatgtgt ctccatttct tgataaaaaa tcaggctgaa
     4921 gtacttttga atcagtgttt gagttgaatt tattgatttg cgaagctact ctatgagaaa
     4981 gagaagcgct acttttagtt gtatctgatg agttatcatt actgcttgtc actccttccg
     5041 ggtggagagg gggggtggtt gaagagttct gtatgttcag aggcctgtgt cccagagaaa
     5101 gagagttatt tgcagatctt tcttgactac tcgtagttaa caggtctgaa cctattccat
     5161 ccgaattatc tggagtatgg ttgaaaacct tttctaaagc tgaattattt gcaaatgtct
     5221 ggacagtatc tgatatagag tgtggtagaa cggaagttac tttttgaact agtgctcttt
     5281 gtggatttag taatgagttt ccgttacggg ataatagatc gtttgtctga gaagtaggag
     5341 actgtgcatc attttctggg ttctcgtgat agctattgtc atatactctg ttgtcaaaaa
     5401 ctctattatc gtaagttcta ttatcattat ttatatgata gtgaattaca ggttgggatg
     5461 ttttctcatt ggctccgggg gttggtccgc catctggcat atcttcggga ggtgtaggga
     5521 ggccatcttg gataggatgc gaatctttta tatcttctaa aatatcatta aggacttttc
     5581 tcaacagctc aggaaacacg gctactgtat ttaagtctct tgacagttta cctataccaa
     5641 atcctttact atcaatagta tttttacgtt gattgcgcaa actatcaaag gcaagttttg
     5701 cctgtgcgtc aactaaatca tctagtttga gagatgaaac agtttcatta cttaacttta
     5761 attcagcaag aatattattc ttgataaatg atgatacttc agtattgagg agtttttgtc
     5821 ccaatgataa agcttcacca gatgtgtgtt gttgtgctaa tagtgcaagt ctgtgctcaa
     5881 tgttttgtcg taattgatgg agtttcttat tctttgccga ccatggtcca aaggcagaat
     5941 cttgggataa tttatctttt ataattagtt ccgaatactg tgcgacaatt ttttgaagtc
     6001 ccttttcgtt ttcaaaatta agaccatttt gatgtactaa ggagtacaaa acatttgaaa
     6061 attcgagaat ctcgcttttg gcatacgcat tcattgagtt ggaataaatg cttcttaaca
     6121 catcttcttt ccctgaggaa gaaaataaaa aatcaagaca tttctgatta aaagacgttg
     6181 caaagttttc tttttcagac actgatgctg gtgtttttag agaagtttgc gaactatgga
     6241 tatcggatat tttgcttttt aactcgctgt attctgttga tgatggtgaa gttgccttat
     6301 ataagaatgt tggcgcttga gtattattta cattatgcat ggcgcacctc agaaatggag
     6361 aaaaagttta tctgtatctg tacatgagct tattgtacta ctcaaaacct ttactaaatt
     6421 atcaaaaata ctattggcat tactgtattt ttgaactaaa gtttgaagat tatttttcat
     6481 tgtttcatct tcggcagaga atccggcatt ccatgcctga tattttgcat tatctagcac
     6541 aacctcgcca tttccaccta gattatctaa gctttttaac atattgtcta ttggggtcat
     6601 gtttatattg acaacatatc ccccgttttt ttcagatacc ttgccgattg ttccacctaa
     6661 ttctgtaagc catttatttg cttgttcctg actaacagta ttatttgctg gatatagcgg
     6721 tttatcttta tattttttct tgagttcttc caatgcgttt ttaagcgagt tgacttgtaa
     6781 tttcacggag tttccgtcgt tacctccggg agagatccag ccggcaagac tggaaagaac
     6841 agcgctaaaa tcttgataca tttgagtata tgaactaacg gcatgttcat atactttcag
     6901 atactgttca ttaatatcat tgatggagtt tgcaatttta gcccacagtt ctctatgaga
     6961 tatcatttga tctccatcaa gctcggcttc tttcggggct gaatgtaata attctcttgc
     7021 gtctttatta attggatatt cgttcctgga aagagtatcc aatagttgag cggatttatt
     7081 tacatccatg ctaatctgag atgaatgtaa tgctatttct tctagcgatg ttttagtcaa
     7141 cgttttttgt gaaagctctt tctttaatgc ctgatttgtt gttctgatat tatgaagggt
     7201 gtcgttgagc atagtaaggg aacttacagg atgagaactg gtcgttgttt ttatatcaga
     7261 attaactgtt tcggttgatg aaccgttggt attgtttgga ctgaatgatg aggtggaaat
     7321 actattagtc agagttgtta tattcatggt tatttcctta caggaagagc catatattat
     7381 tatggctctt ggatatatta agctcgaatg ttaccagcaa tctgactggc tgtcgaattc
     7441 tttgattggg tgatgctgtc aattatattc aataattttt gtattaattg atttgtcgct
     7501 tgggatgctt ctttagatac ttgggatgct tcctctgctt gtttactgct ggcctgactg
     7561 attagttgtt cttcttcttc aagagcagat gcataacgcc ctcctgatgt ggatatatta
     7621 ccagcaacgg ctgatgtgtc cattgttgca cggccaatgt tttgtttttg ctgcgcagaa
     7681 agggtattga gctcgtaagt tcttctctgg gtgtcaattt tatcctgcaa agaaatatcg
     7741 ggagaactaa gagaagtttt atgttcagtt gacagggata tattatctgg cgccagttta
     7801 tttttaccta aaaattttgt gctagagtta gtttgtggtg aggtgatatt tgtatctatt
     7861 tgtttattta accctaattt agaacctgca agctcttttt caagagattg agcagtggca
     7921 aggttctttc ttaaggctcc tttttggtcg ctaatccctg aatgcgtttt tttggcaccg
     7981 atacccgtta tacctacttg tgtgactgct ccagtaatgc ttgatgatag tgctgccagg
     8041 ccttgccgaa caatgttctc tgcagctgat tttgtagcat cgaacgcaat caatgacaat
     8101 tgagagccca atttagtttc tgcagtgcgg agagcagaaa gtagaacggc tacactaata
     8161 attaaagaaa cagcattaga agaaagactg gaaatatcca gagtgttctc tggagtaagg
     8221 gtgtgactca tctctgaaag atctttcatc tttttgtcag taaggtgagc aatgatttct
     8281 gaatcatgct gaacctgctc tgataacttt aaaagttgat catcatttaa tgtggttgtt
     8341 aatacgggat ttttaccgac attaagtgga atatgcgctg caatctgctg ataattttgt
     8401 gatttttgtg tttcggaaga actttgagtt tgttttgtgg atatatctgt atataaaatc
     8461 tgggttgatt ttgtgttttg aatttccata acattctcct tatttgtatc aagcagtagt
     8521 ttgttgcaaa attgcttttg caacatcagt tctattagcc tgagagttgg acattgaggc
     8581 taatagatct gcaattactt cctgcaattg gccgaatttt tctattgctt cactgatata
     8641 ttttgacagt tgttcaactt gatatttcga taatgtcagg tctgctagat ttgtcgacgc
     8701 gctgttctgg aaaacagcag aagcgacact tcctcccgct tgtgtggcag agttaacact
     8761 ttctcctaat aaaactgctt ggtttaaatg ggtggaaata atttgtttgg atattacttc
     8821 atcacccgct gcaccaagaa atttatttaa tctggcaaca gcattagtga ttaaatcgtc
     8881 cagttgagaa gaaaaactct tgagaaactt tggtataagg tctgtgaggg ttttacctat
     8941 tattttgcca atattctctg caagttttgc tgctgcctgt ttaccaacag tggctacgag
     9001 aacgactgct gcaactagga caagagcgcc tgcgattgcc cccagaatag agccaatcat
     9061 tttggctttt ttcgagtcga cgcccaagcc ttcgagcatt tttgtaaatg catctgaaag
     9121 gagtttgatt aagggttcaa tgactgcttt catgatcgga ttcagggctt gttccatgaa
     9181 ggaattgccg gtcgctgctt gtactatagc atccgtaacc ataagagcta aaccaacagc
     9241 tgccagtgct agagaggctc ctccagaaaa agctgctgca acaacactaa cgatagttaa
     9301 aagtgcccca agtattttcc caacacaacc cattactctg ttgagttctt ctgctttacg
     9361 tacttcagca gcatactcat cagattttct ctccatttca gtttttcttg attcttggag
     9421 agactggaat agagccagat cattttttaa agattcttca ttattttttc caactagttg
     9481 aataaaggtt gccatcaatt gagtaacact ggcaagtccg gttaatgatt tctgctgggt
     9541 tgatagctgt tcagcagatg ctgtgtttga aaatgcagaa aaagagtcta tttctttttc
     9601 gagttgcatt gatttatctg taagtttgct atgaattgac agggttttct gctcaatcaa
     9661 tgtcctgtct ttaactgctg cgtctttttt gatagtgagt tgtatttctt cccggcttaa
     9721 ttttttcttt tctggtgaat ctggggcgag ttcggataat cttgtttgaa tttggttaat
     9781 tttattttct aggtctttta ttttagaatc tgcgtttttt agtttattaa tttgtttttc
     9841 atagtctctg gttagtcctt cagtttcaga tagaagagta ttaattttat cggagaattc
     9901 taggtttttt tgctgtcttg cctgttgctg agacttccaa gcagtaattt tatttgttaa
     9961 tgcagttaaa gatttttcac cgagtatttg aataaggttt ccaattaaaa gcgttagttg
    10021 ggaacttgca tttaatgact ttggtgcttt aagttcaggg attaatatat ttgaagttga
    10081 gtgtgcatta gttgtattaa tatttttgtt agcagtaaga tcagcaattg taagagaaaa
    10141 taatttgtta gctgcatcat ttgcagcctg gatagtattg tctccaagct cagtggaagt
    10201 caatattttg tcaagagaca aaccagtggt tgtggtactt acattatgca taataattac
    10261 tccttgatat cctgaattgc gtccaagtat gattgtgctt ttatttttaa tttttcatca
    10321 ttgctgtgtt gaattacgag ttcgaagcac tctttggctt tcaagggggc tttcaaccga
    10381 agctggcatt gtccagtatg gaataccggt gtatagtcat tttttcctaa tgcaaaagcg
    10441 acagcataaa ggtctgctgc ttgttggaac tgttctttta tctgataaat agctgcgagt
    10501 cccataatgt agtctacatt gtaaaagtcg tatatacata aaaatctgaa gaaaacttca
    10561 gcttcctcta ttcttccttt gttgtaaaag tcataagcat atgaataaat gtcatccatc
    10621 atatcatcag gaattgcatt aatatctttc agtgtagcgc cagagttaat tgcatcaatt
    10681 actgcagtag agatgctttc attttcagtg atatttaaag acataaggtc tcctttttgt
    10741 ctgctttact ttaaaaagta taagatttaa tataaacaga tttaatttgt attgctttga
    10801 cggtatacgg cctttagaac ctctgcgctt atttctattt gtaaaacttt tgttgattca
    10861 ttaaatattt tatcgtagac ggaactggat actagatttg taagatcctt catttgtgtg
    10921 ttaaacaaat ccatgtcact gctatagcgt tttaaaatct ttgctctgag gccagatgag
    10981 gcaatctcgt ttagtgctgt atgactgata gatgtttgtg ctgatttttc atcaagtttt
    11041 atatccggga attttttttc aatagctgca aatatgtttt ttttgttatc tgaggtcaaa
    11101 acaactccat tgtgtatggc catttcatct atacttctgt ttatcattga agagacataa
    11161 gaggttcgct cttggctcat ccaacaaaat agttgattcc ccgaattact atcttttatt
    11221 ttgtctatga cgttgtccct gattttattt atattcaccg ggcatccaaa attcttctga
    11281 ggaaagcgtt gttttaagtt aaaaacagat aatattttct ttacaagctt attgtaactt
    11341 aacgaattaa atgatgggcg aggaatcgcg aattcaacct gtggaagtat tttgtttaga
    11401 atttgcatga taccccctat atgttagttc acttctgaag tgatgtttgc aatggttagt
    11461 ttaatacaaa ataaaaattc atacaaagat tcaatgagtt catcaggtga aagatatttt
    11521 ttgtagtgtt tcttggtcaa aacacattga tgagtatcag ggagaagaca gatagaagaa
    11581 ccataatctc tactcaaaga gtagttaagg atgagtatgt gttctggatt aataatattt
    11641 tctgtaaaaa gttcatgttc attaagaagt ccggtcaaaa agatgtattc atcattatct
    11701 gttatacaaa caggtatttg ttcttcgagc aatataaagg cttgattttt atcgttaaaa
    11761 tcaagctttg cacctgttat ttcataaagt ttatcatata gtttgcgaca catttctatg
    11821 gccttctata tattagaatg agagttattc aataaaagct tattgatctg taataagtcg
    11881 tgaatgtaaa gtttggttcc aagatctggc gatttaagag aattgtaata atcaatagtt
    11941 tttattgact tttcgataat ttcttctttt ttatctgttg ctccggtttt taattcgttt
    12001 aatacacgag acatattgct gggtgctgat tttactaagt atgagtggca agcgttgatg
    12061 accgcatcaa agtcttctga agatacattt tcagtaggat gtttgtaaag tgaaattgat
    12121 aaaggcttga atgtttttgg ggtgtggata aggttaattt tattgaaact tttaagatag
    12181 ctacgtcgta attgagtcaa tctggaaaga tcaaaatcag attcaatata cttcattatc
    12241 tcgttataag actgattgag actttcaatg cgttgcctaa ctttatttgc atacgcgtat
    12301 acattgttag gtgtagcaac caatttaacg tcagggagtg gaaagtatat ttcagaattt
    12361 cctgctttaa gaacattaag tgccatacca gcacagtttt cagatttact gattaattta
    12421 taatttgcca attgtctgaa agcatctgct ttcttttggc atataaattt tgacatgttt
    12481 gtttcatcaa ggccaaaaag gttatagctg attttaccat cctttgtttt attatcacct
    12541 gataatggaa tgtatacttt atcagcactg accccccaat aaccataggg gtcttttttt
    12601 tgattagctc tgggaaaaaa tatttttttg ttgttatttt gatcttgttt tgaatttaat
    12661 aagtttctaa cctcttggcc tgcatcaagt ctttcggctg tttggtctga aatcatattt
    12721 agtttgtctt gtttataggt atcgacactg taacctgagc gtttttcaaa aaggtagttt
    12781 atttctgtat cttttgtaac attcccgccg ggataccagc tgagataggt atgagttggc
    12841 tcctgtagta aattgttttt tatactgata ctggtatgcc ctaaatgggt tatgcctcta
    12901 tttccccagc aacgaaaaac taaatgtctt tcaagatttt tggctgtacc gatgccatga
    12961 aaacgctctg cggtattcaa cattaatttg atgtctttac ctgtaaaaga atgttgaatt
    13021 gaatttttga ctgcttcata ggcggcaaac gcactttctg cagatttttc gctagctaat
    13081 gtcttgagga aaagcaataa ggatttttga ttcgctagtc gataattttt tagttgttca
    13141 tttaatatct gtgaatttat ctttgcatta atagtgctta atgttctgaa aaagagatcc
    13201 tttaggttga atttctgtgc taccaacata ggtccatgct ctgtatcttc aacgcgtata
    13261 ggcccttttg tattgcttgc gtcaatgaaa ttgctaattt tgagaatcat actttattaa
    13321 ctctccatta cgtggtgatt gtggggatgt aaaatgtatt tgatatataa tatattcaat
    13381 taataaattt agaaacttga gcctgttaac ataatcaaat tttcttttgt tgtacataat
    13441 atgtacctcg tgagcatatg tagtgctcgt ttcatcatga aatcccacaa gttaaagtgt
    13501 ctgatatatc aggctcggag tgttatagaa aaagagagaa ccctgttgaa taagtatctt
    13561 ttttgttgta aaacgaggta caaaactgca atcaatgccc catttatgac ggaggataat
    13621 taaatgcaca taactaattt gggattgcat caggtttcat ttcaaagcgg agattcctat
    13681 aaaggcgcag aagaaaccgg aaacccaaaa ggtgtaagcg tgatttcata tcaaagagtt
    13741 aaaaatggag agagaaataa gggggtcgaa gcgcttaatc gattatattt acagaaccaa
    13801 acaagcttaa cagggaaagg ccttttgttt gcgcgtgaca gagcagccgt tttttacgag
    13861 gcaataaagt tggcaggtgg tgatacgtca aaaattaaag ccatgatgga acaattagat
    13921 acctataaac ttggtgaagt tgataaaaga aatattaatg agcttaataa agtaataagt
    13981 gaagagataa gagcacagct aggcattaaa aataaaaagg aattgcaatc ggaaataaaa
    14041 caaatattta ctaaatattt aaataataag aattgggaac cgatcaacaa aaatattaat
    14101 tatcatggga aaaattatgg ttttcaatta acacccgcct ctcatatgaa gatagggaat
    14161 aaaaatatat ttgtcaaaga gtataatgga aaaggaattt gttgtgcttc tacgagagag
    14221 agtcagcata ttgcgaatat gtggctttca aaagtggtgg atgacgaggg gaaggaaatt
    14281 ttttcgggta tcagacatgg agttatatca gcctatggat tgaaaaaaaa ctcatctgaa
    14341 agagctgttg ctgctcgtaa taaagctgag gagttagcaa gcgcagcatt atatagcaga
    14401 cgagaattat tatcacaggc tttgtctggt aaaacagtag atttaaagat tgtttcgact
    14461 tctcttctga cgccgaccag tttaactggg ggagaggaga gtatgttaaa agatcaggta
    14521 aaggcattaa aagggttaaa ctctaaaagg ggggaaccaa cgaaattatt aattcggaat
    14581 agtgatggtc ttctgcaaga agtaaacgtt aatctaaaag tggtgacatt taactttggt
    14641 gtaaatgaat tagcgctaaa aatgggctta ggctggagga atgttgacaa gcttaatggt
    14701 gaatcaatat gttctttgtt gggggataat ttcctcaaaa atggtgtgat tggaggttgg
    14761 gctgcagaag ctatagaaaa aaatccacca tgtaagaatg atgtgatata tctggctaac
    14821 cagataaaag atattgtaaa caagaagtta caaaaaaatg ataatggaga gccgtataaa
    14881 ttgtcacaaa gaatggctct attggcttat actataggtg ctgtaccttg ctggaattgt
    14941 aagagtggga aggacagaac aggtatgcag gatgctgaaa ttaaaagaga aataataaga
    15001 aaacatgata cagggcagtt ttctcaatta aatagtaaat tatcctcaga agagaaaaga
    15061 ttattttcta ctattctaat gaatagtggt aatatggaaa tccaagagat gaatacgggt
    15121 gtgcctggaa ataaagttat gaaaaaatta ccgctatctt cgttagagct gtcttatgct
    15181 gaaagaatag gggaccccaa aatatggaat atggtgaaag ggtattcgtc atttgtataa
    15241 gaggaatata tggaagattt agcagatgtt atttgccgag ccttgggtat cccttcaatt
    15301 gataccgatg atcaagcaat catgcttgat gatgatgtgt taatatatat tgaaaaagaa
    15361 ggagattcaa taaatttatt atgccctttt tgtgcattgc ctgaaaatat aaatgatctt
    15421 atatatgcgt tgagtctaaa ttactcagaa aagatatgtt tagctactga tgatgaaggt
    15481 ggaagtctaa ttgcgcgatt agatcttacc gggataaatg aattcgagga tatatatgtt
    15541 aatacagaat attatatttc gcgtgtgcgt tggctgaagg atgaatttgc gcgaagaatg
    15601 aaggggtatt aatgtcccgt ttcgtattta ttttgctgtt ttttatccca tatttaggga
    15661 gagctgattg ttgggataag gctggtgaaa ggtacaacat tccatcgagt ttgttaaaag
    15721 cgattgcgga aaaagaatcc agatttaata aatctgcagt caatgttaat aacaatggaa
    15781 gtaaagatta tggtataatg caaataaatg actttcattc taaaagactt agagaaatgg
    15841 gatatactga ggaaatgcta attagtcatc catgtctttc tgtacattat gcagcaaagt
    15901 tattaaatga gtttatgctg atgtatggaa gagggtggga ggctgttggt gcctataatg
    15961 caggaacatc tccgaaaaag aaaaaagaaa gattaaagta cgcagaggat atctataaac
    16021 gctatttgcg gatagcagca gagagcaagc agaataatcg aaggatataa gaggatttat
    16081 gtctgaggca aagaactcaa atcttgcacc attcaggtta ttagtgaaac tcacaaacgg
    16141 tgtcggggat gagtttccgt tatattatgg gaataactta attgttctcg gacggacgat
    16201 agaaacactt gagtttggaa acgataattt cccagaaaat attattccag taacagatag
    16261 taaatcggat ggtataattt atttggccat aagcaaagat aatatctgtc aatttagtga
    16321 tgaaaaaggt gaacaaattg atatcaattc acaatttaac tcatttgaat atgacggagt
    16381 ttcttttcaa cttaaaaata tgatggagga taaatcgaga gttcatatac taaacggaat
    16441 gtataataaa ccctcggtgt tttttttctt tgctgttatt gttgtgttaa ttataatttt
    16501 ttccctctct ttaaagaaag atgaagtaaa agaaattgct gaaattatag atgataagag
    16561 atatggaata gtcaacacag gacagtgcaa ttatattctt gccgaaacac aaaatgatgc
    16621 ggtatgggca tctgtagcct taaataaaac cggatttacc aaatgcagat atattctggt
    16681 gtccaacaaa gaaataaata gaattcaaca atatatcaat caacgattcc catatatcaa
    16741 tttgtatgtg ttgaatttag tgagtgataa agcggaatta ctggtatttt taagtaaaga
    16801 aagaaatagt tcaaaagata ccgaacttga gaaattgaaa aatgctctga ttgttgaatt
    16861 tccgtatatc aaaaatatta aattcaacta tcttagcgat cataatgcaa gggaggatgc
    16921 taaaggtatt ttcacaaagg taaatgtcca gtataaagag atttgtgaag ctaataaagt
    16981 tacatattct gtcagagaag aactgacaga tgaaaagctt gagctcatta atagactgat
    17041 ttctgatcat aaaaatgtat atggagatca gtatattgag ttttctgtac ttttgataga
    17101 tgatgatttt aaaggtaaat catatcttaa cagcaaagac agttatgtaa tgttgaatga
    17161 taaacactgg ttttttttag ataaaaataa gtgaggataa aatgagtgtt acagtaccgg
    17221 ataaagattg gactctgagt tcattatctg aaacttttga tgatggaact caaacattac
    17281 aaggtgaact aacattggca ctagatgcat tagctagaaa tccttcgaat ccacagttgc
    17341 tggctgaata ccaaagtaaa ttatctgaat atacattata taggaacgcg caatctaata
    17401 ctgtgaaagt gattaaggat gttgatgctg caattattca aaacttcaga taatagggag
    17461 cattcatgaa ttacatttat ccagtcaatc aggttgatat tatcaaagcc agtgattttc
    17521 aatctcaaga gatatcaagt ctggaagacg tcgtgtcggc taaatatagt gatattaaga
    17581 tggatacaga tattcaagta tcacaaataa tggagatggt aggcaatcca gaatcattaa
    17641 atccagaatc tttggccaag ttacagacga cgctctcaaa ttattcaata ggagtatcat
    17701 tagctggcac gttagcaaga aaaacagttt cggctgttga aactttatta aagtcttaat
    17761 ttatatgatt aggtataaag gttttatttt attcttgttg ctgatgttga ttggatgtga
    17821 gcaacgtgaa gagttaattt ctaatttatc tcaaagacag gcaaatgaaa taatatctgt
    17881 gctagaacgc cataatatta ctgctagaaa agttgatgga ggtaaacagg ggatctcggt
    17941 acaagtcgaa aaggggacat ttgcatcggc agttgatttg atgcgcatgt acgatttgcc
    18001 aaatccggag agagttgata tctcacaaat gtttcctaca gattcattag tgtcttctcc
    18061 aagagctgaa aaggcccgtt tatatagtgc tattgagcaa cggctggaac agtctttagt
    18121 ttctattggt ggtgttattt cggcaaaaat acatgttagc tatgatcttg aagaaaaaaa
    18181 tatatcttca aaaccgatgc atatatcagt aatcgctata tatgactcac cgaaagagtc
    18241 tgaactatta gttagtaata ttaagcgatt tttgaaaaac accttttctg aggttaagta
    18301 tgaaaatata tctgtcatat taactccgaa agaagaatat gtttatacaa atgtacaacc
    18361 tgttaaggaa attaaatcgg aatttttaac aaatgaagta atatatgtat ttctcgggat
    18421 ggctgtacta gttgtcactc ttttggtatg ggcattcaaa acagggtggt tcaagagaaa
    18481 caaaatatga taagaatgga tggaatttat aaaaaatatc tttcaataat ttttgatcca
    18541 gcgttctata taaatagaaa tcggttgaat ttgccttctg aactgttaga aaatggcgta
    18601 atcagaagtg agattaataa tctcataatt aataaatatg atctaaattg cgatattgaa
    18661 cctttaagcg gggtaaccgc tatgtttgtt gccaactgga atttacttcc agctgttgct
    18721 tattttatcg gtagtcaaga gtctcggtta attaatcata gtgagatggt tattagttat
    18781 tatgggggca aaatcagtaa gcaaggggag gccgctattc gaagtggttt ttggcatctt
    18841 attgcgtgga aggaaaatat atctgttggt atatatgaaa gaataaactt attatttaat
    18901 ccaattgctt tggagggaaa ttatacgcct gtagaaagaa atttgtctcg attaaatgaa
    18961 ggtatgcaat atgcaaaaag gcactttacc ggtatccaga catcatgcct atgatggtgt
    19021 tgttataaag agaattgaaa aggaattatg taaaaccata aaagatagag acactgagag
    19081 taagaagaaa gcaatttgtg ttataaagga tgctacgaaa aaagctgaat cactgcgtat
    19141 agatgctgta tgtgatggtt atcaaattgg tatacagact gcttttgagc atattattga
    19201 ctatatttgt gaatggaagt taaagcaaaa tgaaaacaga agaaatattg aggactatat
    19261 aactagtttg ctttcagaaa atttacatga tgagaggatc attaacactt tattggagca
    19321 gtggcttagt tcgttgcgta atacagtaac agaattaaaa gtggttttgc ccaagtgtaa
    19381 tttagcactg aggaaaaagt tagaacttga tctccataaa tatcgctcag atgtcaagat
    19441 tattttaaaa tattctgaag ggaataatta tatattctgt agtggtaatc aagtagtgga
    19501 gtttagtcct caggatgtaa tatcaggtgt taaaatagaa cttgcggaaa aattaacaaa
    19561 aaatgataaa aaatatttca aagagttagc acataagaaa ctaagacaaa ttgcagaaga
    19621 cttgttaaaa gagaatcctg ttaatgatta atcaaataaa tgcaagcaat gctctgcagc
    19681 aaagattaaa tagtgaagaa tttgttaatt taaatgagag attatcatct tcgcaatcat
    19741 ttgatgagga tataatctat gaaataatgc aatatttctc acagagtgaa ttaaattcca
    19801 tagataatga tgaactgcac aataagattg aacaattatt caattcacgt tttccttatc
    19861 ttacagctgc acaaaaaagc tcacttctta ataaactcat agatgcaaat caatatgtcg
    19921 atttgcatga gggattttat gcatcattgt ccatatataa caatattgat ttctatatta
    19981 agactacaac cttcgattca ttaattagtg tctttgacgc agggagagag gcagatgatt
    20041 cgacatggta gtaataagtt gaaaatattt attttaagta tattgctatt aacactgagt
    20101 gggtgtgctt taaagtcatc atctaattct gaaaaagaat ggcatattgt tcctgtaagt
    20161 aaggattatt tttctattcc aaatgattta ttatggtcgt ttaatacaac caataaaagt
    20221 ataaatgttt actctaaatg tattagtggt aaggcggttt atagttttaa tgcaggtaaa
    20281 ttcatgggca actttaatgt taaggaagta gatgggtgct tcatggatgc acaaaagata
    20341 gctatagata aactattttc tatgctgaaa gacggggttg ttttaaaagg taataagata
    20401 aatgatacca tccttataga gaaggatggg gaagttaaat taaaattaat tcgagggata
    20461 taattgtatt gtgagtaaat ataaaggtct aaatacaagt aatatgtttt acatttactc
    20521 tagtggacat gaaccagtta acgttgagct tgtaaaagat aaagaacgta acataattga
    20581 gctggctcca gcatggaagg gttttttttt gtccgaaatc aaaatataaa attcagtgat
    20641 aatgtgaact atcattatcg ctttaacatt aatagttgtg caaaatttct tgctttttgg
    20701 gactatttca gtggtgctct agtagagcat agccatgctg aaaaatgtat tcatttctat
    20761 catgagaatg atcttcggga tagttgtaat accgaatcga tgcttgataa gttaatgtta
    20821 agattcatct tttcatctga ccagaatgta agtaatgcac tggctatgat acgaatgact
    20881 gagtcatatc atttggttct ttatcttctg agaactattg agaaagaaaa ggaggtaaga
    20941 ataaagagtc tgacagagca ttatggtgtg tcggaggctt attttagatc tttatgtaga
    21001 aaagctttag gggcaaaagt caaagaacaa ctgaacactt ggcggttagt aaatggttta
    21061 ttagatgtat ttttgcacaa tcaaacaatc acttctgctg ccatgaataa tgggtatgca
    21121 tcaacatccc atttttcaaa tgagataaaa acacgactag gcttttctgc aagagaatta
    21181 tcgaatatta cttttttagt gaaaaaaata aatgaaaaaa tttaatatta aatctttgac
    21241 tctcttgatt gtattgttac cattgatagt taatgctaat aatattgact ctcatttatt
    21301 agaacaaaat gacatagcga aatatgttgc tcaaagtgat acggtaggat ctttttttga
    21361 acgtttttct gcgttattaa attatcctat tgttgttagc aaacaagctg caaaaaagag
    21421 aatatctggg gagtttgatt tatctaatcc agaagaaatg cttgaaaaac tgacattact
    21481 tgtgggactc atatggtata aagatggaaa tgcattatac atatatgact ctggagaatt
    21541 aataagcaaa gttattttgc tggagaatat atcacttaat tatcttattc agtatctgaa
    21601 ggatgctaac ctttatgatc acagatatcc tataagagga aacattagtg ataaaacatt
    21661 ctatatatct ggtcctccgg cattggttga actggtagct aatacagcaa ctttgcttga
    21721 taagcaggtt tcttctattg ggacagataa agttaatttt ggggttataa aattaaaaaa
    21781 tacatttgtt agtgacagaa cttataatat gagaggggag gatattgtca tcccaggggt
    21841 ggctactgtt gtagaacgtt tattaaataa tggtaaggct ctgagtaaca gacaagccaa
    21901 aaatgatccg atgccaccct ttaatattac acagaaagta tctgaggata gtaatgactt
    21961 ttcatttagt tcagttacca atagttctat attagaggat gtatcactta ttgcatatcc
    22021 agaaaccaat tctatattag tcaaaggtaa tgatcagcaa atacagatta ttcgcgatat
    22081 tatcactcag ttagatgtag ccaaacgaca tatagaatta tcattatgga taatcgatat
    22141 tgataaatct gaattaaata accttggcgt taattggcaa ggaacggctt catttggtga
    22201 ttcgtttggt gcgtcattta atatgagctc atcagcatcg ataagcactt tggacggcaa
    22261 taagtttatt gcttcagtta tggcattaaa tcagaaaaag aaagcaaatg tagtttcaag
    22321 gccggtgatt ttgacacaag agaatattcc ggctatattt gataataaca ggacttttta
    22381 tgtgtcatta gttggtgagc gcaattcttc gttggagcat gttacgtatg gaacattgat
    22441 aaatgttata cctcgctttt catctcgggg acaaattgag atgtctttaa cgatagagga
    22501 tggaacagga aatagtcagt caaattacaa ttataataat gaaaatacat ctgtccttcc
    22561 agaagttgga aggaccaaaa tcagtacaat agcgagagtt ccacaaggca aaagtttgtt
    22621 gataggtggt tatacgcatg aaacgaactc taacgaaata gtatctattc cttttctttc
    22681 ctctattcct gtaatcggta atgtgttcaa gtataaaaca agtaatatca gtaatattgt
    22741 aagggttttt ctgattcaac cccgtgagat aaaggagtca agttattata atactgcaga
    22801 atataagagc ctcatatctg aaagagaaat acaaaagact acacaaataa tcccatctga
    22861 aactactttg ctggaagacg aaaaatcatt ggtttcatac ttaaattact aactataaag
    22921 taggtgatgt atgcttgatg ttaaaaatac aggagttttt agctctgcat tcattgataa
    22981 gttgaatgca atgacaaatt cagatgatgg agatgagact gctgatgcag agcttgattc
    23041 tggcttggct aatagcaagt atattgactc atctgatgag atggcttccg ctctttcgtc
    23101 atttataaac agaagagacc ttgagaaact gaaaggaaca aatagtgata gtcaggaacg
    23161 tattttagat ggggaagaag atgaaattaa tcacaagatt tttgatttaa agagaacttt
    23221 aaaagataac cttcctctag atcaggattt tatagacaga ctaaagagat attttaaaga
    23281 tccaagtgat caagtcttag cattaaggga acttttgaat gaaaaagatc ttactgctga
    23341 acaagtcgaa tcattaacta aaattattaa tgagataata tcaggtagtg aaaaaagtgt
    23401 taatgctgga ataaattcag ctatacaggc taaattattt ggcaacaaaa tgaaacttga
    23461 accacagctt ttgcgtgcat gttatcgtgg ttttatcatg gggaacatat caacaacaga
    23521 tcagtatata gaatggcttg gtaattttgg ttttaatcac agacatacaa ttgtgaattt
    23581 tgtagagcag tcactgattg tagacatgga ttctgagaaa ccgagctgta atgcttatga
    23641 gtttggtttt gtgttatcta aattaattgc aattaagatg attagaactt cagatgtaat
    23701 ttttatgaag aaactggaat cctcaagctt gctaaaagat ggcagtttaa gtgcagagca
    23761 gctattgcta actttattat atatttttca atatccaagt gaaagtgagc aaattcttac
    23821 ttctgttata gacgtatcac gagccagtca tgaggattct gtagtgtatc aaacatatct
    23881 atcttctgtt aatgaaagtc ctcatgatat atttaaaagt gaaagtgaaa gagaaattgc
    23941 gatcaatatt ctacgagagc ttgtcacaag tgcatacaag aaagagcttt ctagataaca
    24001 ggagatgaaa gtgatccagt cttttcttaa gcaagtaagt actaagcctg aattaattat
    24061 attagtgctt atggttatga tcattgctat gttgatcata ccattaccta cataccttgt
    24121 tgatttttta atagggctta atattgtact tgctattctc gtttttatgg gatcatttta
    24181 tattgaaaga atattgagtt tttctacatt cccttcggtt ttgttgataa cgacattatt
    24241 tcgtctcgct ttatcaataa gtactagtcg actaatttta gttgatgcgg atgcgggtaa
    24301 aattattact acatttggcc agtttgtcat tggtgatagt ctggctgtag gttttgttat
    24361 tttttcaata gtaactgttg tgcagttcat tgttataaca aaaggatctg aaagggttgc
    24421 ggaggtagct gctcgctttt ctcttgacgg tatgccgggg aaacaaatga gcattgatgc
    24481 ggatttaaag gctggaatta ttgatgccgc aggagctaaa gaaagacgca gtattctgga
    24541 acgtgagagt caattgtatg gttcatttga tggagcaatg aagttcatta aaggtgatgc
    24601 aattgctggc atcattatta tttttgtcaa tttaatagga ggtatttctg ttggtatgag
    24661 tcaacatgga atgtctctat ctggtgcatt atctacttat accatactaa ctattggtga
    24721 cggattggtc tctcagattc ctgctctgtt aatttctata agtgcaggat ttatcgttac
    24781 aagagttaat ggtgatagcg ataatatggg acgtaacata atgtcccaga tatttgggaa
    24841 tccttttgtt ctcattgtta catcagctct tgctttggcg atagggatgt tgccaggttt
    24901 tccgtttttt gtttttttcc tgatagcagt tactttgacg gctttattct attataaaaa
    24961 ggtcgtagaa aaagaaaaaa gtctgtctga gtctgattct agtggctata ctggtacatt
    25021 tgatattgat aatagccatg actcatcttt ggcaatgata gaaaatctgg atgctattag
    25081 ttcagaaacc gttcctttaa ttttattatt tgccgaaaat aagataagtg caaatgatat
    25141 ggaaggtctt attgaaagga taagaagtca gttctttatt gattatggtg tcaggcttcc
    25201 aactatttta tatagaacaa gcaatgagct taaggtcgat gatattgttt tgttaataaa
    25261 tgaggtgcgt gccgatagtt ttaatatata ctttgacaaa gtgtgtatta cagatgaaaa
    25321 tggagatata gatgctctag gtattcctgt tgtttcaact tcatataatg aacgtgttat
    25381 ttcttgggta gacgtttcat atacagaaaa tctaactaat attgatgcta aaattaaaag
    25441 tgctcaagat gaattttatc accagttgtc acaagcttta ttaaacaaca taaatgagat
    25501 ttttggtata caagaaacaa aaaatatgtt agatcagttt gaaaatcggt atcctgatct
    25561 attaaaggaa gtcttccgac atgtgactat acagagaatt tctgaggtat tacaaagatt
    25621 gcttggagaa aatatttctg ttcgcaattt aaaacttatt atggagtctt tggcgctttg
    25681 ggctcccaga gaaaaagatg ttataacatt agttgaacat gtccgtgcat cactttctag
    25741 gtatatttgt agtaaaatag ctgtttctgg tgagattaaa gttgtgatgc tttccggata
    25801 tattgaggat gcaataagaa aagggataag gcaaacctct ggtggctctt tcttgaatat
    25861 ggatatagag gtttcggatg aggtaatgga aactttagca catgctttga gagaattgag
    25921 aaatgcaaaa aaaaatttcg ttcttttggt atcagtagat atacgtaggt ttgttaaaag
    25981 acttatagat aacagattta agagtatact cgttatatcg tatgctgaga ttgatgaagc
    26041 atataccatt aatgtattaa agactattta gtgaggttta aatatgagta acattaattt
    26101 agttcaatta gttagagata gtcttttcac gattggttgt cctccatcaa ttattacaga
    26161 tttagacagc cattcagcga ttaccatttc tttggatagt atgcctgcta ttaatatagc
    26221 tcttgtcaat gagcaggtta tgttatgggc taattttgat gcaccaagtg atgtaaaact
    26281 tcagtcatct gcttataaca tattaaattt aatgttaatg aactttagtt atagcattaa
    26341 tgagctggta gagcttcatc gttctgatga gtacttacag ttaagagttg ttattaaaga
    26401 tgactacgtt cacgatggga ttatttttgc agagattctg catgagtttt atcagaggat
    26461 ggaaatctta aatgaggtct tataatcaat gagctatata aaattgctca ctcaattatc
    26521 ttttcctaat agaatctcgg ggccaatctt ggaaacaagt cttagcgatg tttcgattgg
    26581 tgagatttgt aacattcagg ctggaattga aagtaatgaa attattgcaa gagctcaggt
    26641 tgtaggattt catgatgaaa aaacaatatt aagcttgatt ggaaattctc gtggactttc
    26701 acggcaaacg ttgattaagc ccactgccca gtttcttcat acgcaagtcg gccgtggatt
    26761 attgggagca gtagtcaatc ctttagggga ggttactgat aagtttgctg ttacggataa
    26821 cagtgaaatt ctttatcgac ctgtagataa tgctcctccg ctatatagtg aaagggctgc
    26881 aattgagaag ccttttttaa caggtgttaa ggttattgat tctttactca cgtgtggtga
    26941 aggacagcga atggggattt ttgcgtcagc tggttgtggc aaaacttttc tcatgaatat
    27001 gctcattgaa catagtggtg ctgatatata tgttattggg ttaattggtg agcgaggtcg
    27061 agaggttaca gaaacggttg attatttgaa aaactctgag aaaaaaagca ggtgtgtttt
    27121 agtatatgca acttcggatt actcttcggt tgatcgttgt aatgctgcat atatagccac
    27181 tgctatagcc gaatttttta ggactgaagg acataaagta gcgcttttta ttgattcatt
    27241 aacaaggtat gccagagcat tacgtgatgt ggccttagcc gctggagaat cacctgccag
    27301 aagaggctat ccggtttcgg tttttgatag cttacccaga cttcttgaaa ggccaggaaa
    27361 gttaaaggca ggtggctcta ttactgcatt ttacactgtt cttttggagg atgatgattt
    27421 tgctgatcca ttagctgaag aggtaagatc cattttagat ggacatatat atttgagcag
    27481 aaatctagcc caaaaaggac aatttcctgc aattgattcc ttaaaaagta taagcagggt
    27541 atttacacag gttgttgatg aaaaacatcg tattatggcc gctgcatttc gggagctact
    27601 ttctgaaata gaagagctaa ggacaattat tgactttggt gaatacaaac cgggggagaa
    27661 tgcctctcag gataaaatct acaacaaaat atctgttgtt gagagttttc tgaagcaaga
    27721 ttatcgactc ggttttactt atgagcagac aatggagctt attggtgaaa caattagata
    27781 aggttttaaa aatcaaagat aaatatcaac ggtctgttaa attaattgaa gcacacatat
    27841 taacactaaa aaaaaaactc tttgtatcga gatgtggagg cattagataa aaggattata
    27901 tacttccagc aactggagaa tgacttggaa cctgtaggcg ctcagagtgt tagtcagctt
    27961 ttcaatacaa gacgtaagat cgctatagta aaaaagcata ttatccaata tcagtctgaa
    28021 cgaattcttc taaagggaag aattgaagaa attcaaaaag atattgatga ggcaaatgct
    28081 tctaaaagaa aactgcttca taaagagagt aaaatatgta aacggattgg cttgattaaa
    28141 agaaataact ttgccaaaca gttgatactt gatgagttat cacaagagga tatgaagtat
    28201 ggcattagat aatataaacc taaattttag tagtgacaaa cagatagaaa aatgtgagaa
    28261 actatcttca atagataata ttgatagtct cgttttgaag aagaagagga aggttgaaat
    28321 tcctgagtac tctttaattg catctaacta ttttactatt gataagcact ttgaacataa
    28381 gcatgataaa ggagaaattt atagtggcat taaaaatgcg ttcgaactta gaaacgaacg
    28441 agcgacatat tctgatattc cagaatcaat ggccattaaa gaaaatattt tgataccaga
    28501 tcaagatatc aaagcaaggg aaaaaataaa tatcggcgat atgaggggga tcttttcata
    28561 taataagagt ggaaatgcag acaagaactt cgaaagaagt catacttctt ctgtaaaccc
    28621 tgataatctg ctagaatctg ataatagaaa tggtcaaatt ggtttaaaaa atcatagctt
    28681 gtctattgat aagaatattg ctgacatcat ttctttacta aatggaagtg ttgctaaatc
    28741 atttgagctg cctgtaatga ataaaaatac tgcagacata accccatcca tgtcattgca
    28801 agaaaaatca atagttgaaa atgataaaaa tgtttttcaa aaaaatagtg aaatgactta
    28861 ccactttaaa cagtgggggg ctggacattc tgttagtatt tcagtagagt ctggttcttt
    28921 tgttctaaaa ccgtcagatc aatttgtagg aaataaactt gacttaattt tgaaacaaga
    28981 tgctgagggt aattacagat ttgatagcag tcaacataat aaggggaata aaaataatag
    29041 tacaggatat aatgaacaga gtgaagaaga atgctaagaa ttaaacattt tgacgctaac
    29101 gaaaaactac agattttata tgcaaagcaa ctctgcgagc gtttttcgat tcagacattc
    29161 aaaaataaat ttacaggcag tgaaagttta gtcactctta cttccgtgtg tggggattgg
    29221 gtaattcgta ttgatacatt atcttttttg aaaaaaaaat acgaggtatt ttcaggattt
    29281 tctacacaag aatctttact gcatttatca aaatgtgtct ttatagagtc gtcatctgta
    29341 ttttcgattc cagaactgtc tgataagatt actttccgga tcacgaatga aatccagtat
    29401 gcaactactg ggagtcattt atgctgtttt tcctcttctt taggtattat ttattttgac
    29461 aagatgccgg tattacgtaa tcaagtttct cttgacttat tgcatcatct tttagagttt
    29521 tgcttaggtt catctaatgt aaggctggct actttaaaaa gaattcgtac tggtgatata
    29581 atcatagttc agaaacttta taatttatta ttgtgtaatc aagttattat tggggattat
    29641 attgtgaatg ataataatga ggcaaaaatt aatctgtcag aaagtaatgg tgagtcagat
    29701 cacacagaag tttctttggc attattcaat tatgatgata tcaatgtaaa agtggacttt
    29761 atccttttag aaaaaaagat gacaatcaat gaactaaaaa tgtatgtaga aaacgaatta
    29821 ttcaagtttc ccgatgacat agttaaacat gtaaatatta aagtaaatgg ttctttggtt
    29881 gggcatgggg aacttgtttc tattgaggat ggttatggta tcgagattag ttcttggatg
    29941 gtaaaggagt aatctcatgc tgagtgatat gtccctcatc gctacacttt ctttttttac
    30001 gctattacca tttttagttg ctgctggtac ttgttatata aagttttcta ttgtttttgt
    30061 aatggtacga aatgcacttg gtttgcagca ggttccatca aatatgactc tcaatggtat
    30121 tgcattgatt atggctttgt tcgtgatgaa acccattatt gaagctggtt atgaaaatta
    30181 tctaaatggc ccgcaaaaat ttgatacaat atcagatatt gtccgttttt ctgattctgg
    30241 cttgattgaa tacaagcaat acttgaaaaa gcatacagat cttgagttgg cacgtttttt
    30301 ccaaaggtct gaagaagaaa atgctgattt aaagagtgca gagaataatg attattcatt
    30361 gttttccctc ttgcctgcat atgcgctaag tgaaattaaa gatgcgttca aaataggttt
    30421 ttatttatat ttgccattta ttgttgttga tcttgttatt tcaagcattt tacttgcact
    30481 tggtatgatg atgatgagtc caatcacaat atcagtacca ataaaacttg tattatttgt
    30541 tgcgcttgat gggtggggga tcctgtctaa ggccttgatt gaacaatata tcaatgttcc
    30601 tgcttagatg atggaggtaa tgtgtctgat atagtttata tgggtaataa ggctctttat
    30661 ttaatcctta tcttttcctt atggccagta ggtatagcta cggttattgg attaactatt
    30721 ggtttattac agacagtgac tcaacttcaa gagcagacac ttccttttgg tataaagctt
    30781 ataggtgtct caatatcttt gctacttctt tctggatggt atggtgaggt tttattgtct
    30841 ttttgtcatg aaataatgtt tttaattaag agtggggttt gatggacatt tcaagctggt
    30901 tcgaaagtat tcatgtgttt ttaatactcc tgaacggcgt tttttttaga ttggctccat
    30961 tatttttctt tcttccattt ttaaataacg gtataatttc tccatctatt agaatacctg
    31021 tgatttttct tgttgcatca ggattaatta cttctggtaa ggtagacata ggttcttctg
    31081 tttttgaaca tgtttatttc cttatgttca aggaaataat tgttggcctc cttctctctt
    31141 tttgcttgtc tcttcccttt tggatatttc atgctgttgg tagcattatt gacaaccagc
    31201 gtggggcaac gcttagtagt tcaattgatc ctgccaatgg tgttgatacg tctgagttgg
    31261 caaaattttt caatcttttt tctgcagttg tatttctata cagtggtgga atggtcttta
    31321 ttttagaatc catacaattg tcttataata tatgcccgtt attttctcaa tgttctttcc
    31381 gtgtctcaaa tatcttaaca tttctgactt tattggcaag tcaggctgtt attttagcca
    31441 gtcctgttat gatagtattg ttactatcag aagtattact tggtgtatta tcgagatttg
    31501 ctccgcagat gaatgctttt tccgtatcat taactattaa aagtttactt gcaatattta
    31561 ttattttcat ctgttcttct actatttact tttctaaagt tcaatttttc ctcggtgaac
    31621 ataaattttt cacaaatcta tttgttagat aaaatattat ggcaaataaa acagaaaagc
    31681 cgacacctaa aaaactaaag gatgccgcaa aaaaaggaca gtcatttaaa tttaaggatt
    31741 taacgactgt tgttattatt ctggtaggga catttactat aatatcattc ttttccttaa
    31801 gtgatgtaat gctcttatac agatatgtaa taattaatga cttcgaaatt aatgagggta
    31861 aatacttttt tgcagtggtt attgtctttt ttaagataat tggcttccca ctttttttct
    31921 gtgttctttc ggctgtgttg ccaacattgg ttcaaacaaa gtttgttctt gcgactaaag
    31981 ctatcaagat tgatttttca gtattaaacc ctgttaaagg gttaaaaaaa atatttagta
    32041 taaagacaat aaaagaattt ttcaaaagca ttctgcttct tattattcta gcattaacaa
    32101 cctatttctt ttggattaat gaccgaaaaa taattttttc tcaggtgttt tctagtgttg
    32161 atggcttata tcttatttgg ggggggctgt ttaaggatat aatattattt ttcttggcat
    32221 tttctatttt tgttattatt cttgactttg tgattgagtt cattttatac atgaaagata
    32281 tgatgatgga taaacaggag ataaaaagag aatatataga gcaagaggga cactttgaga
    32341 caaagtcgag aaggcgtgag ttgcatatcg agattctttc agagcagact aaatctgata
    32401 tacgtaattc aaaattagtg gtaatgaacc cgactcatat tgcaattggt atttatttta
    32461 atccagaaat agcgcctgca ccttttattt ctctcattga aactaaccag tgtgccttgg
    32521 ctgtcagaaa atatgcaaat gaagttggta taccaactgt gcgtgatgtg aaattagcta
    32581 gaaaactata taaaacacat acaaaatata gttttgttga ttttgaacac ttggatgaag
    32641 tcctacgtct tattgtttgg cttgagcagg ttgaaaacac tcattaataa gtaaaggaga
    32701 tgtgttatat gggagttaat ttctgtaata aaataggtat tgatcagtct gaatttgaaa
    32761 tagaatcttc catcattaac tccattgcta atgaggtatt gaacccaata tctttcctta
    32821 gcaacaagga tataataaat gttttactca gaaagatttc atctgaatgt gacctagtta
    32881 gaaaagacat ttatcgttgt gctctggagt tggtcgttga aaaaacacct gatgatttat
    32941 aatcattcat aataactctt ttgctgatat aaatattttt gttgaagata tttatgtagt
    33001 cacaggatca attgtagaaa attgttatgt ttttgttagg aactgcacta aataatatat
    33061 gataagacag caaaaacgac tgaccataat ccttctttta ttaggagttg ataagagaga
    33121 ctattcatcc tgtaatgtta aaactctgtt atatagtatt cgcgattatg caaagtctgt
    33181 aaatgatcat gagatactaa ccgagagtaa tcgtttatta tctcattgca tttcagatag
    33241 taacggagct ttctttaaaa gtagtaaata cgttcccctt aagtacttac gtaaaaggag
    33301 aatagcacga aagataccaa atgattagca ctttgctagt aagcttgctg gtaaactata
    33361 ttgaccgttg ggtttgtttt gagactgtaa tttaaattgt gtaattgcct gtttttgata
    33421 tgtttactcc aacaacggag acaggtaaat tatggacgaa aagaagctta aagcccttgc
    33481 ggctgaactg gctaaaggcc tcaaaactga ggccgacctc aatcagtttt cccgtatgct
    33541 gacgaaatat cccggctctt aaaacgatag tggctcaggc tttcattttt cagcgttccc
    33601 cagaagcttt ccatcggagc gttgtcgtaa cagttacctt tacgcgacat tgatgttttc
    33661 aga
//
LOCUS       DADKXM010000030        33588 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.030, whole genome shotgun sequence.
ACCESSION   DADKXM010000030 DADKXM010000000
VERSION     DADKXM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.030"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..613
                     /locus_tag="JQR05_001418"
     CDS             <1..613
                     /locus_tag="JQR05_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5323621.1"
                     /translation="MDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQ
                     ELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHAS
                     LQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGE
                     VADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(610..1424)
                     /locus_tag="JQR05_001419"
                     /pseudo
     CDS             complement(610..1424)
                     /locus_tag="JQR05_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(1424..3040)
                     /locus_tag="JQR05_001420"
     CDS             complement(1424..3040)
                     /locus_tag="JQR05_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY5323622.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3335..4009
                     /locus_tag="JQR05_001421"
     CDS             3335..4009
                     /locus_tag="JQR05_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5323623.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4048..5709)
                     /locus_tag="JQR05_001422"
     CDS             complement(4048..5709)
                     /locus_tag="JQR05_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY5323624.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JQR05_001423"
     CDS             complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JQR05_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY5323625.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JQR05_001424"
     CDS             complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JQR05_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY5323626.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7163..7495
                     /locus_tag="JQR05_001425"
     CDS             7163..7495
                     /locus_tag="JQR05_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY5323627.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7497..8747)
                     /locus_tag="JQR05_001426"
     CDS             complement(7497..8747)
                     /locus_tag="JQR05_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY5323628.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            8812..9912
                     /gene="cbrA"
                     /locus_tag="JQR05_001427"
     CDS             8812..9912
                     /gene="cbrA"
                     /locus_tag="JQR05_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY5323629.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9873..11210)
                     /locus_tag="JQR05_001428"
     CDS             complement(9873..11210)
                     /locus_tag="JQR05_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5323630.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JQR05_001429"
     CDS             complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JQR05_001429"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAY5323631.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRARFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPFGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12430..13047)
                     /locus_tag="JQR05_001430"
     CDS             complement(12430..13047)
                     /locus_tag="JQR05_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY5323632.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13031..13909)
                     /locus_tag="JQR05_001431"
     CDS             complement(13031..13909)
                     /locus_tag="JQR05_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY5323633.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JQR05_001432"
     CDS             complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JQR05_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY5323634.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14873..15529
                     /locus_tag="JQR05_001433"
     CDS             14873..15529
                     /locus_tag="JQR05_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323635.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15575..16387)
                     /gene="yidA"
                     /locus_tag="JQR05_001434"
     CDS             complement(15575..16387)
                     /gene="yidA"
                     /locus_tag="JQR05_001434"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY5323636.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16501..16908)
                     /locus_tag="JQR05_001435"
     CDS             complement(16501..16908)
                     /locus_tag="JQR05_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY5323637.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17129..19543)
                     /gene="gyrB"
                     /locus_tag="JQR05_001436"
     CDS             complement(17129..19543)
                     /gene="gyrB"
                     /locus_tag="JQR05_001436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY5323638.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19572..20645)
                     /gene="recF"
                     /locus_tag="JQR05_001437"
     CDS             complement(19572..20645)
                     /gene="recF"
                     /locus_tag="JQR05_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY5323639.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPEFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASCLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20645..21745)
                     /gene="dnaN"
                     /locus_tag="JQR05_001438"
     CDS             complement(20645..21745)
                     /gene="dnaN"
                     /locus_tag="JQR05_001438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY5323640.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21750..23153)
                     /gene="dnaA"
                     /locus_tag="JQR05_001439"
     CDS             complement(21750..23153)
                     /gene="dnaA"
                     /locus_tag="JQR05_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY5323641.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23450..23530
                     /locus_tag="JQR05_001440"
                     /pseudo
     CDS             23450..23530
                     /locus_tag="JQR05_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23760..23900
                     /gene="rpmH"
                     /locus_tag="JQR05_001441"
     CDS             23760..23900
                     /gene="rpmH"
                     /locus_tag="JQR05_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY5323642.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23917..24276
                     /gene="rnpA"
                     /locus_tag="JQR05_001442"
     CDS             23917..24276
                     /gene="rnpA"
                     /locus_tag="JQR05_001442"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY5323643.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24240..24497
                     /gene="yidD"
                     /locus_tag="JQR05_001443"
     CDS             24240..24497
                     /gene="yidD"
                     /locus_tag="JQR05_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY5323644.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24500..26146
                     /gene="yidC"
                     /locus_tag="JQR05_001444"
     CDS             24500..26146
                     /gene="yidC"
                     /locus_tag="JQR05_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY5323645.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26252..27616
                     /gene="mnmE"
                     /locus_tag="JQR05_001445"
     CDS             26252..27616
                     /gene="mnmE"
                     /locus_tag="JQR05_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY5323646.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEACQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADTTGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            27858..27932
                     /gene="tnaC"
                     /locus_tag="JQR05_001446"
     CDS             27858..27932
                     /gene="tnaC"
                     /locus_tag="JQR05_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY5323647.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            28153..29568
                     /gene="tnaA"
                     /locus_tag="JQR05_001447"
     CDS             28153..29568
                     /gene="tnaA"
                     /locus_tag="JQR05_001447"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY5323648.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQGMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            29659..30906
                     /gene="tnaB"
                     /locus_tag="JQR05_001448"
     CDS             29659..30906
                     /gene="tnaB"
                     /locus_tag="JQR05_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY5323649.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            31038..32213
                     /gene="mdtL"
                     /locus_tag="JQR05_001449"
     CDS             31038..32213
                     /gene="mdtL"
                     /locus_tag="JQR05_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY5323650.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALEIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            32188..33147
                     /gene="yidZ"
                     /locus_tag="JQR05_001450"
     CDS             32188..33147
                     /gene="yidZ"
                     /locus_tag="JQR05_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY5323651.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            33304..33549
                     /locus_tag="JQR05_001451"
                     /pseudo
     CDS             33304..33549
                     /locus_tag="JQR05_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
BASE COUNT         8094 a         8882 c         8480 g         8132 t
ORIGIN      
        1 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
       61 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
      121 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
      181 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta
      241 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
      301 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
      361 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
      421 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
      481 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
      541 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
      601 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
      661 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
      721 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
      781 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
      841 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
      901 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgag
      961 gcttctgcga caattgctgc cgggttggag tagttgagca tccaggcgtt tggtgaatat
     1021 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1081 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1141 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1201 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
     1261 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1321 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
     1381 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1441 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
     1501 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     1561 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
     1621 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     1681 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
     1741 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     1801 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     1861 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     1921 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     1981 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
     2041 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2101 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2161 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2221 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
     2281 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
     2341 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
     2401 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
     2461 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     2521 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     2581 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     2641 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     2701 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
     2761 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     2821 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     2881 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     2941 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3001 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3061 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat
     3121 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3181 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3241 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
     3301 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
     3361 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
     3421 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
     3481 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
     3541 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
     3601 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
     3661 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
     3721 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
     3781 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
     3841 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
     3901 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
     3961 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
     4021 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
     4081 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
     4141 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
     4201 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
     4261 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
     4321 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
     4381 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
     4441 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
     4501 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
     4561 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
     4621 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
     4681 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
     4741 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
     4801 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
     4861 agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
     4921 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
     4981 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
     5041 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
     5101 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
     5161 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
     5221 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
     5281 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
     5341 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
     5401 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
     5461 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
     5521 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
     5581 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
     5641 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
     5701 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
     5761 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
     5821 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
     5881 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
     5941 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
     6001 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
     6061 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
     6121 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
     6181 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
     6241 gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
     6301 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
     6361 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
     6421 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
     6481 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
     6541 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
     6601 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
     6661 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
     6721 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
     6781 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
     6841 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
     6901 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
     6961 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
     7021 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
     7081 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
     7141 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
     7201 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
     7261 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
     7321 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
     7381 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
     7441 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
     7501 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
     7561 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
     7621 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
     7681 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
     7741 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
     7801 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
     7861 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
     7921 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
     7981 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
     8041 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
     8101 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
     8161 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
     8221 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
     8281 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
     8341 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
     8401 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
     8461 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
     8521 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
     8581 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
     8641 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
     8701 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
     8761 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
     8821 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
     8881 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
     8941 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
     9001 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
     9061 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
     9121 gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
     9181 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
     9241 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
     9301 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
     9361 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
     9421 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
     9481 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
     9541 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
     9601 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
     9661 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
     9721 ccggagaaga gcaacgccgc ttactggcgc gccacacgca aactgcgttt aaaactcttc
     9781 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
     9841 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
     9901 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
     9961 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    10021 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    10081 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    10141 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    10201 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    10261 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    10321 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    10381 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    10441 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    10501 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    10561 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    10621 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    10681 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    10741 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    10801 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    10861 gtggcaaagc cctggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    10921 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    10981 caagcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    11041 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    11101 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    11161 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    11221 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    11281 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    11341 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    11401 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    11461 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttcctga agtaccgcgt
    11521 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    11581 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    11641 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    11701 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    11761 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    11821 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    11881 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    11941 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    12001 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    12061 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    12121 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    12181 cggcgatggc gctcatcagg attggaccgc cacgataaaa tctggcgcga tacatcactt
    12241 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    12301 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    12361 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    12421 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    12481 ggtgcgttct acggactgcc cggcgcgata gagatcgctg cctaagcccg ccccaacaca
    12541 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    12601 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    12661 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    12721 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    12781 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    12841 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    12901 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    12961 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    13021 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    13081 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    13141 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    13201 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    13261 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    13321 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    13381 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    13441 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    13501 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    13561 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    13621 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    13681 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    13741 cttcacgcca gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc
    13801 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    13861 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    13921 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    13981 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    14041 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    14101 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    14161 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    14221 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    14281 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    14341 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    14401 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    14461 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    14521 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    14581 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    14641 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgtgt
    14701 gtaatgtagt acaaacttat attgttgtac tacaatttag atcaaaaaaa gaacaatgcg
    14761 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    14821 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    14881 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    14941 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    15001 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    15061 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    15121 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    15181 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    15241 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    15301 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    15361 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    15421 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat
    15481 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    15541 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    15601 caaacgccac gccatcttca aggttggatt ttgtgacaaa gttcgccact tctttcaccg
    15661 aaggaatagc gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt
    15721 tttcctgatc gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg
    15781 atttcacacc cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac
    15841 ttttcagcac ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga
    15901 ggatggcggg ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct
    15961 ccgcttcgca gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc
    16021 tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga
    16081 cttcgcgaga gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca
    16141 cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc
    16201 ccggctgttc catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg
    16261 tcgttagcac gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg
    16321 gtgaaatggt gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga
    16381 tagccatgag ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa
    16441 aatcagtaca acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc
    16501 ttagcggaag agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc
    16561 ttgtgctgac acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc
    16621 cagtaaactg gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc
    16681 atttgtgccg agcgccgatt ccagttgctc gccgctaact gattgatttc tctgttgatt
    16741 actcaaccag gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac
    16801 ctgaatgcca ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc
    16861 tttcagaaag gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt
    16921 acgtgttgac ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa
    16981 agggcggaga tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc
    17041 ttatccggcc tacaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc
    17101 atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt
    17161 tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact
    17221 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    17281 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    17341 agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    17401 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    17461 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    17521 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    17581 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    17641 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    17701 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    17761 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    17821 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    17881 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    17941 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    18001 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    18061 tgatgatgct gtgataacgt agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    18121 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    18181 tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    18241 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    18301 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt
    18361 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    18421 ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt
    18481 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    18541 gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg
    18601 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    18661 cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    18721 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg
    18781 ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg
    18841 gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt
    18901 ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    18961 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    19021 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    19081 ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    19141 gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    19201 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    19261 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    19321 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    19381 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    19441 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    19501 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    19561 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    19621 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    19681 gcttttaagc agctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    19741 tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga
    19801 cgtaaggcgc acatcaacag cttaagctgt ccacgcgata aggtatcttc caccggcgca
    19861 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta agttagctgg
    19921 cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg
    19981 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcagccata
    20041 tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    20101 atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    20161 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaattc aggttcgttg
    20221 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    20281 aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc
    20341 ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    20401 gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg
    20461 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    20521 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    20581 gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg
    20641 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    20701 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    20761 tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg
    20821 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct
    20881 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc
    20941 gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg
    21001 gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt
    21061 cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc
    21121 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    21181 gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg
    21241 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    21301 gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    21361 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    21421 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    21481 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    21541 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    21601 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    21661 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    21721 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    21781 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    21841 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    21901 gtcagctctt tcgccagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    21961 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    22021 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    22081 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    22141 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc
    22201 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc
    22261 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    22321 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    22381 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    22441 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    22501 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    22561 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    22621 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    22681 gccacctggc gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    22741 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    22801 tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    22861 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    22921 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    22981 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    23041 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    23101 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    23161 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    23221 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    23281 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    23341 gtttctcaca gattttctct atttatccac aggactttcc agaactcgct aagtgtaaac
    23401 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    23461 caaaaattgg cttaattgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    23521 gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat
    23581 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg
    23641 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    23701 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    23761 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    23821 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    23881 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    23941 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    24001 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    24061 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg
    24121 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    24181 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    24241 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    24301 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    24361 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    24421 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    24481 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    24541 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    24601 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    24661 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    24721 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    24781 ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    24841 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    24901 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    24961 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    25021 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg
    25081 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    25141 actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa
    25201 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    25261 gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    25321 ctataccgct aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact
    25381 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    25441 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    25501 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    25561 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    25621 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    25681 tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc
    25741 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    25801 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    25861 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    25921 gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat
    25981 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    26041 actgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    26101 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    26161 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    26221 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    26281 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    26341 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    26401 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    26461 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    26521 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    26581 gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc
    26641 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    26701 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    26761 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    26821 agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag catgtcaggg
    26881 tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc
    26941 gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac
    27001 cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    27061 cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    27121 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc
    27181 cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat
    27241 caccgtggta cgcaataaag ccgataccac cggcgaaacg ctgggaatga gtgaagtgaa
    27301 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    27361 ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg
    27421 tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    27481 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    27541 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    27601 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    27661 ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta
    27721 atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca
    27781 catttaaaca atttcagaat agacaaaaat tctgagtgta ataatgtagc ggcgtgtctt
    27841 tcgaggataa gtgcattatg aatatcttac atatatgtgt gacctcaaaa tggttcaata
    27901 tcgacaataa gattgtcgat caccgccctt aatttaccct tctgtagcca tcaccagagc
    27961 caaaccgatt agattcaatg tgatcgatta gtttgctata tcttaatttt gccttttgca
    28021 aaggccatct ctcgtttatt tacttatttt agtaaatgat ggtgcttgta tatatacatg
    28081 gcgaattaat ggatattgca gatgtaatat tcacagggat cactgtaatt aaaataaatg
    28141 aaggattatg taatggaaaa ctttaaacat ctccctgaac cgttccgcat tcgtgttatt
    28201 gagccagtaa aacgtactac tcgcgcttat cgtgaagaag caattattaa atccggtatg
    28261 aacccgttcc tgctggatag cgaagatgtg tttatcgatt tactgaccga cagcggcacc
    28321 ggggcggtaa cccaaggtat gcaggcagcg atgatgcgcg gcgacgaagc ctacagcggc
    28381 agccgcagct actatgcgtt agccgagtca gtgaaaaata tctttggtta tcaatatact
    28441 attccgactc accagggccg tggcgcagag caaatctata ttccggtact gattaaaaaa
    28501 cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg cattctctaa ctatttcttt
    28561 gataccacgc agggccatag ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa
    28621 gaagccttcg atacgggcgt gcgttacgac tttaaaggca actttgacct cgaaggatta
    28681 gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt atatcgttgc aaccatcacc
    28741 agtaactctg caggtggtca gccggtttca ctggcaaact taaaagcgat gtacagcatc
    28801 gcgaagaaat acgatattcc ggtggtaatg gactccgcac gctttgctga aaacgcctat
    28861 ttcatcaagc agcgtgaagc agaatacaaa gactggacca tcgagcagat cacccgcgaa
    28921 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
    28981 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
    29041 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
    29101 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
    29161 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
    29221 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc atatcccggc agaccagttc
    29281 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
    29341 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
    29401 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
    29461 gaagccttta aacatgtaaa agagaacgcg gcgaatatta aaggattaac ctttacctac
    29521 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
    29581 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
    29641 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
    29701 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
    29761 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
    29821 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
    29881 accatcacca aagatttaat aggtaacacc tggaacatta tcagcggtat taccgttgcc
    29941 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
    30001 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
    30061 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
    30121 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
    30181 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
    30241 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
    30301 ctgattattt gctatggcaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
    30361 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
    30421 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
    30481 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
    30541 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
    30601 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
    30661 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
    30721 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
    30781 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
    30841 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
    30901 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
    30961 tgtccaaatg gcaactcgcc ttatcctcct tcaccacgta tgctttgcgt caccttacta
    31021 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
    31081 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
    31141 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
    31201 tgctatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg
    31261 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
    31321 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtggtg gcgttcgcca
    31381 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
    31441 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
    31501 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
    31561 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
    31621 atagcgagtc gctgcttaat cgttttttcc tcagccgtgt tgttatcacc accctcagcg
    31681 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatggggt
    31741 ttgagcgcgg agaatacgcc accattatgg cgctgaccgc tggcgtcagc atgacctttt
    31801 cattctcgac gccatttgcg ctggaaattt ttaagccacg tacgttgatg atcacctcgc
    31861 aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
    31921 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
    31981 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
    32041 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
    32101 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
    32161 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
    32221 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
    32281 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
    32341 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
    32401 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
    32461 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
    32521 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
    32581 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
    32641 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
    32701 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
    32761 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
    32821 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
    32881 gctatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcgcaacc cgacaatctg
    32941 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
    33001 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
    33061 ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt
    33121 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aatgcgaccc aatttcacaa
    33181 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
    33241 tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga
    33301 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
    33361 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
    33421 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
    33481 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
    33541 ttttctatgt aagcgcccca tcagcgacgt cttgtgaaaa ttgtcctg
//
LOCUS       DADKXM010000031        33502 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.031, whole genome shotgun sequence.
ACCESSION   DADKXM010000031 DADKXM010000000
VERSION     DADKXM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.031"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            873..3158
                     /gene="nrdA"
                     /locus_tag="JQR05_001452"
     CDS             873..3158
                     /gene="nrdA"
                     /locus_tag="JQR05_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY5323652.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3392..4522
                     /gene="nrdB"
                     /locus_tag="JQR05_001453"
     CDS             3392..4522
                     /gene="nrdB"
                     /locus_tag="JQR05_001453"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY5323653.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHQTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4522..4776
                     /gene="yfaE"
                     /locus_tag="JQR05_001454"
     CDS             4522..4776
                     /gene="yfaE"
                     /locus_tag="JQR05_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY5323654.1"
                     /translation="MARVTLRITGTQLQCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4830..5486)
                     /gene="inaA"
                     /locus_tag="JQR05_001455"
     CDS             complement(4830..5486)
                     /gene="inaA"
                     /locus_tag="JQR05_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY5323655.1"
                     /translation="MTGDSSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGK
                     KLYVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRA
                     LLVTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRH
                     IYVKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM
                     "
     gene            5648..5890
                     /locus_tag="JQR05_001456"
                     /pseudo
     CDS             5648..5890
                     /locus_tag="JQR05_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5932..7008)
                     /gene="glpQ"
                     /locus_tag="JQR05_001457"
     CDS             complement(5932..7008)
                     /gene="glpQ"
                     /locus_tag="JQR05_001457"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY5323656.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7013..8371)
                     /gene="glpT"
                     /locus_tag="JQR05_001458"
     CDS             complement(7013..8371)
                     /gene="glpT"
                     /locus_tag="JQR05_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY5323657.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8644..10272
                     /gene="glpA"
                     /locus_tag="JQR05_001459"
     CDS             8644..10272
                     /gene="glpA"
                     /locus_tag="JQR05_001459"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY5323658.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEETGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGGRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10262..11521
                     /gene="glpB"
                     /locus_tag="JQR05_001460"
     CDS             10262..11521
                     /gene="glpB"
                     /locus_tag="JQR05_001460"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY5323659.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRVTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11518..12708
                     /gene="glpC"
                     /locus_tag="JQR05_001461"
     CDS             11518..12708
                     /gene="glpC"
                     /locus_tag="JQR05_001461"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY5323660.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12901..13803
                     /locus_tag="JQR05_001462"
     CDS             12901..13803
                     /locus_tag="JQR05_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY5323661.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPISFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13844..14314)
                     /locus_tag="JQR05_001463"
     CDS             complement(13844..14314)
                     /locus_tag="JQR05_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY5323662.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14369..15571)
                     /locus_tag="JQR05_001464"
     CDS             complement(14369..15571)
                     /locus_tag="JQR05_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY5323663.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPPEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGNEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15658..16200)
                     /locus_tag="JQR05_001465"
     CDS             complement(15658..16200)
                     /locus_tag="JQR05_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323664.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            16479..16904
                     /gene="nudI"
                     /locus_tag="JQR05_001466"
     CDS             16479..16904
                     /gene="nudI"
                     /locus_tag="JQR05_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY5323665.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16943..17545)
                     /locus_tag="JQR05_001467"
     CDS             complement(16943..17545)
                     /locus_tag="JQR05_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY5323666.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSEGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17853..18992
                     /gene="arnB"
                     /locus_tag="JQR05_001468"
     CDS             17853..18992
                     /gene="arnB"
                     /locus_tag="JQR05_001468"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY5323667.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18996..19964
                     /gene="arnC"
                     /locus_tag="JQR05_001469"
     CDS             18996..19964
                     /gene="arnC"
                     /locus_tag="JQR05_001469"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY5323668.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19964..21946
                     /gene="arnA"
                     /locus_tag="JQR05_001470"
     CDS             19964..21946
                     /gene="arnA"
                     /locus_tag="JQR05_001470"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY5323669.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFADFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21943..22833
                     /locus_tag="JQR05_001471"
     CDS             21943..22833
                     /locus_tag="JQR05_001471"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY5323670.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22833..24485
                     /gene="arnT"
                     /locus_tag="JQR05_001472"
     CDS             22833..24485
                     /gene="arnT"
                     /locus_tag="JQR05_001472"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY5323671.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            24482..24823
                     /gene="arnE"
                     /locus_tag="JQR05_001473"
     CDS             24482..24823
                     /gene="arnE"
                     /locus_tag="JQR05_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY5323672.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLWLGL
                     ALACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFI
                     IGGIVILGSTV"
     gene            24823..25209
                     /gene="arnF"
                     /locus_tag="JQR05_001474"
     CDS             24823..25209
                     /gene="arnF"
                     /locus_tag="JQR05_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY5323673.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25203..25475)
                     /gene="pmrD"
                     /locus_tag="JQR05_001475"
     CDS             complement(25203..25475)
                     /gene="pmrD"
                     /locus_tag="JQR05_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY5323674.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25580..26935)
                     /gene="menE"
                     /locus_tag="JQR05_001476"
     CDS             complement(25580..26935)
                     /gene="menE"
                     /locus_tag="JQR05_001476"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY5323675.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26932..27894)
                     /gene="menC"
                     /locus_tag="JQR05_001477"
     CDS             complement(26932..27894)
                     /gene="menC"
                     /locus_tag="JQR05_001477"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY5323676.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27894..28751)
                     /gene="menB"
                     /locus_tag="JQR05_001478"
     CDS             complement(27894..28751)
                     /gene="menB"
                     /locus_tag="JQR05_001478"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY5323677.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28766..29524)
                     /gene="menH"
                     /locus_tag="JQR05_001479"
     CDS             complement(28766..29524)
                     /gene="menH"
                     /locus_tag="JQR05_001479"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY5323678.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29521..31191)
                     /gene="menD"
                     /locus_tag="JQR05_001480"
     CDS             complement(29521..31191)
                     /gene="menD"
                     /locus_tag="JQR05_001480"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY5323679.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFGSHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSCLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31280..32575)
                     /gene="menF"
                     /locus_tag="JQR05_001481"
     CDS             complement(31280..32575)
                     /gene="menF"
                     /locus_tag="JQR05_001481"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY5323680.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAALGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32654..32959)
                     /gene="elaB"
                     /locus_tag="JQR05_001482"
     CDS             complement(32654..32959)
                     /gene="elaB"
                     /locus_tag="JQR05_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY5323681.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33014..33475)
                     /locus_tag="JQR05_001483"
     CDS             complement(33014..33475)
                     /locus_tag="JQR05_001483"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5323682.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARDVIQA"
BASE COUNT         8142 a         9017 c         8344 g         7999 t
ORIGIN      
        1 gatactccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
       61 gatatcatat ccctggcata aatcaatggc cgccgcgaca ccgttagcag agaaaagaga
      121 tgcaatcatt gacgggagta gagataaata ctccttgcgt agaaagataa tccgcatatt
      181 aatctgacca tcattttata taagacggca tatttaaacg cattatacaa tatgggttat
      241 ctttcttttg taaattgtcc aacccgatgc aatggctatt ttgttattat ttgatgaata
      301 atatcagtgc gtcataattc aagttaataa ccttaaggga taccagttat atttaaacta
      361 aattaaagta atgaataatt ttcttataat ataaggtaaa ttaacaaaat ggcttcgcat
      421 ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
      481 tatattgacc acaactgata catcagatta tgtgatgact cgtgcttaga tcaatttttg
      541 caatcattag caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
      601 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
      661 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
      721 agttatgcac ttgcaagagg gtcattttca cactatcttg cagtgaatcc caaacatacc
      781 ccctatatat agtgttctaa gcagcttccc ttactacagg tagtctgcat gaaactattg
      841 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
      901 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
      961 cagaaggact gcataacgtt tcgatttccc aggtcgagct gcgctcccac attcagtttt
     1021 atgacggtat caagacctct gacatccacg aaactattat caaggctgcc gcagacctga
     1081 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcgatc ttccacctgc
     1141 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
     1201 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
     1261 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
     1321 agctggaagg caaatatctg gtacagaacc gcgtgaccgg cgaaatctac gaaagcgccc
     1381 agttccttta tattcttgtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
     1441 tgcaatacgt gaagcgtttt tacgacgcgg tttccacatt taaaatttcg ctgccgacgc
     1501 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
     1561 gcggtgacag cctggattcc atcaacgcca cctccagcgc gattgtgaaa tatgtttccc
     1621 agcgtgccgg gatcggcatc aacgccggac gtattcgtgc gctgggcagc ccgattcgcg
     1681 gtggcgaagc gttccatacc ggctgtatcc cgttctacaa acatttccag acagcggtga
     1741 aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac gctgttctac ccgatgtggc
     1801 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggggttgaa ggcaaccgcg
     1861 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgc ctgctgaaag
     1921 gtgaagatat caccctgttc agcccgtccg acgtaccggg gctgtacgac gcgtttttcg
     1981 ccgatcagga agagtttgaa cgcctgtata ccaaatatga gaaagacgac agcatccgca
     2041 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
     2101 gtcgtatcta tattcagaac gttgaccact gtaataccca tagtccgttt gatccgacca
     2161 tcgcgccagt tcgccagtct aacctgtgcc tggaaattgc cctgccgacc aaaccgctga
     2221 acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
     2281 gcgcaattaa tagcctggat gaactggaag agctggcgat tctggcggtt cgtgcacttg
     2341 acgcgctact ggattatcag gattacccga tcccggccgc caaacgtgga gcgatggggc
     2401 gtcgtacgct gggtattggt gtgattaact tcgcttacta tctggcgaag cacggtaaac
     2461 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcg attcagtatt
     2521 acctgctgaa agcgtctaat gagctggcta aagagcaagg cgcgtgcccg tggtttaacg
     2581 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatgcca
     2641 tcgctaatga gccgctgcat tacgactggg aagcattgcg tgaatcaatc aaaacgcacg
     2701 gcctgcgtaa ctccacgctt tctgccctga tgccgtccga gacctcttcg cagatctcta
     2761 acgccactaa cggtattgaa ccgccgcgcg gttacgtcag catcaaagcg tcgaaagacg
     2821 gtattttgcg ccaggtggtg ccggactacg agcacctgca cgacgcctat gagctgctgt
     2881 gggaaatgcc gggtaacgat ggctacctgc aactggtagg tatcatgcag aaatttatcg
     2941 atcagtcgat ctctgctaac accaactacg atccgtcacg tttcccgtca ggaaaagtgc
     3001 cgatgcagca gttgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
     3061 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
     3121 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
     3181 taaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
     3241 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     3301 taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     3361 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     3421 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     3481 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     3541 tccggaagaa gttgacgtct cccgcgaccg tatagattac caggcgctgc cggagcacga
     3601 aaaacacatc tttatcagca acctgaaata tcagacgctg ctggattcca ttcagggtcg
     3661 tagcccgaac gtggcgctat tgccgcttat ttctattccg gaactggaaa cctgggtcga
     3721 aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     3781 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     3841 tgcggaaggg atctccagct attacgatga actgatcgaa atgaccagct actggcacct
     3901 tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta accgttagcc tgcgcgagct
     3961 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga tccgcttcta
     4021 cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag gtaacgccaa
     4081 gattattcgc ctgattgccc gcgatgaagc cctgcaccag accggtactc agcatatgct
     4141 gaatctgctg cgcagcggcg cggatgatcc ggagatggcg gaaattgccg aagaatgtaa
     4201 gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact gggcggatta
     4261 tctgttccgt gacggttcga tgattggtct gaataaagat attctctgcc agtacgttga
     4321 atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     4381 caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     4441 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcggaagtgg acaccgacga
     4501 tttgagtaac ttccagctct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     4561 cagtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgtggcggtt
     4621 gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cccgactggt cgcaggtcag
     4681 gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     4741 tgccgggcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc cagatacgac
     4801 gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     4861 tcccagtccg ctttagggat cggctccaga tatttttcca gctggcggaa gtcatgatta
     4921 atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa accagcttct
     4981 gccttacctt ctgttttcac atagatatgg cgaacatagc aacagccatg ctgacgatta
     5041 acgctatgca ttttcttaaa cgccagtgcc actgctttca acatggcttg ccgtacttcg
     5101 tcagaataag gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     5161 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     5221 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     5281 acaatcgttg gtcggccgaa cggataacgt actgaatgaa acagatgatg cgtcatgcgc
     5341 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     5401 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5461 tactttgctg aactatcgcc tgtcattttt attaacgaca atgactatag gtggttacct
     5521 gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct acaggtgtga
     5581 caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc actaatagtg
     5641 cgcccagcag ataaaatgtg aatttaatca gtcggataca gcgatgaatt ttattcgaca
     5701 gggattaggc atcgccttac aaccagagtt aacgctgaaa agcattgcag gtgaattgtg
     5761 ttccgttcct cacgaaccaa ccttctatcg acagatttcg ttgctgacta aagaaaagcc
     5821 ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag tggcgattgg
     5881 aaaaatttga tatgagacag gatggcgcat gaacgccatc ctgcagtaac attactcttt
     5941 attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta cacccgcttt
     6001 gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca gtttatctga
     6061 ccgcacggta taaggatgca ctaccagttt attctgctga gcatcttgca ccatgccagt
     6121 gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat ccggaccaat
     6181 accatctgca tattccgcca cctgtttcat ggcacccggc ttaaacatcc agtcgtagtt
     6241 gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt cggtataggc
     6301 aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat tcttaatacg
     6361 cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac cggtgtaacc
     6421 atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct gatgatggaa
     6481 ccacggcgct ttgatttctg gatagatacc gatatttttc ccggtagagt gatttaaccc
     6541 ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact tacccattgg
     6601 gaaacgcccc ggataagtct gtactttttt accgttttca atatcgaaac cttcggtaaa
     6661 tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac cgtctttgcg
     6721 cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat ggtcatgcag
     6781 aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat ccgctccctg
     6841 cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac tggcaccgcg
     6901 atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc ccatgactat
     6961 agtgctcatc atgatcgcca tgctaaggtt tttcagcttc aatttcatgc cattagcctc
     7021 cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc gccaatcatc acaacaatca
     7081 acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca tcccagccga
     7141 agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc aggtaaccaa
     7201 acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt tccagcgcat
     7261 gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc atacaaatca
     7321 tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg atggtcacca
     7381 gtgtcataaa gaagacgccg gttgccccac ggttaccacg gaagactttg tccgacatcc
     7441 agccgcacag cagggtgccc ggaatacccg catactcata gaagaagtag gcccaggagg
     7501 atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag tcgaggatgc
     7561 cgtaacgcag cagataaacg aacacgttgg cgatggcgat gtaccacagc agtttgttcg
     7621 gcagcacgta ctgcatgaag atttgcttcg cggtcagctc ctgttccgct ttttcgttat
     7681 agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag gattgcgggg
     7741 tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa gcaggcatat
     7801 agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc agcggcggaa
     7861 taccaccacc gacgttgtgc gcacagttcc acactgacac aatgccgcca cgttctttct
     7921 gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc tggaaccaac
     7981 cgcagaggaa caacagtaca aacatcaccg caatgctcga cgtcgcccat ggcacaaagc
     8041 ccataaacaa catcactgcc gccgccagaa tcaaacctgc gggcaggaaa acgcgcggat
     8101 tcgagcgatc cgataccgaa cccatgatga atttcgaaaa tccataagca atcgagatcc
     8161 ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg ctcaaccaga taaggcatag
     8221 caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat atccccagga
     8281 aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcggcaggt aagcgcgctt
     8341 tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg tggtcttatt
     8401 tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc cgcgatgtta
     8461 agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa gatttacaaa
     8521 atgttcaaaa tgacgcatga aatcacgttt cactttcgaa ttatgagcga atatgcgcga
     8581 aatcaaacaa tttatgtttt tactatggct aaatggtaaa aaacgaactt cagagggata
     8641 acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg cggcgcaacg
     8701 ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt ggttgagcgc
     8761 cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag cggtgcgcgc
     8821 tatgcagtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca gatcctgaaa
     8881 cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct gccggaagat
     8941 gacctctcat tccaggccac ttttattcgc gcctgcgaag aaacagggat cagcgcagaa
     9001 gctatagacc cgcagcaagc gcgcattatc gaacctgccg ttaacccggc actgattggc
     9061 gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc aaacatgctg
     9121 gatgccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg gctgattcgt
     9181 gaaggcgcga cggtgtgcgg tggtcgtgta cgtaaccatc tcaccggcga aactcaggcc
     9241 cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat tgccgaatat
     9301 gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat ggatcaccgc
     9361 attaaccagc atgtgatcaa ccgctgccgt aaaccttccg acgccgatat tctggtgcct
     9421 ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa cgagattgac
     9481 gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga aaaactggcc
     9541 cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc gctggttgcc
     9601 agcgatgacg acccgagcgg acgtaacgtc agccgtggca tcgtgctgct cgaccatgct
     9661 gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat gacctatcgg
     9721 ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac gcgcccctgt
     9781 acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac cttgcgtaaa
     9841 gtcatctccc tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg cgatcgcacg
     9901 cctgcctggc tgagcgaagg ccgtctgcac cgtagcctgg tatgtgagtg cgaagcggta
     9961 actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct gctggattta
    10021 cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc ctgccgcgct
    10081 gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga gcaactttcc
    10141 accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga cgcactgcgc
    10201 gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa ggagcagaaa
    10261 gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct gtggcctgca
    10321 actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg cgttgcattt
    10381 ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg tggcagacat
    10441 tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact ctcttctcgg
    10501 gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg aaagcggtgc
    10561 gcaattgcag ggcagcgtag aactggctca ccagcggatt acgccgctcg gcactttgcg
    10621 tgtaacctgg ttaagttcgc cagaagttcc cgtctggccg ctacccgcga agaagatatg
    10681 tgtagtgggg ataagcgggc tgatggattt ccaggcgcat cttgcggcgg cttcgttgcg
    10741 cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg atgtcctgcg
    10801 caataacgcc accgaatttc gcgcggtgaa tatcgcccgt ttccttgata atgaagaaaa
    10861 ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa tgatcctgat
    10921 gcccgcctgc ttcggcctgg ccgatgacaa actgtggcgt tggttgaatg aaaaactacc
    10981 ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc gtctgcaaaa
    11041 ccagttacag cgccagtttg tgcgccaggg tggcgtgtgg atgccgggcg atgaagtgaa
    11101 aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc acgccgatat
    11161 tccgctacgt ccacgtttcg cggttctcgc cagcggcagt ttctttagtg gcggactggt
    11221 agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac aaaccgccac
    11281 gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc agttcggtgt
    11341 aaccactgat gagacgctac gcccgtcgca ggcagggcaa accattgaga acctgtttgc
    11401 catcggttcg gtgctgggcg gatttgatcc catcgcccag ggatgcggcg gcggtgtttg
    11461 tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag gccaacaatg
    11521 aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc ctgcccggtg
    11581 agcagggtga atcccggtta tccagggcca aaacaagccg ggccggatgg cgagcgtctg
    11641 cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa ctgcaaacgt
    11701 tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg cgcgcgggcg
    11761 aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac cgacctgatg
    11821 ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct gaaaccggtg
    11881 cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc gaaatactcc
    11941 ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca atataaagac
    12001 caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt aggtaaagat
    12061 ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa agaaaaatgc
    12121 tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca ggcaattacg
    12181 aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat tgccacctcc
    12241 tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt cgacaacaaa
    12301 ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct ggacgaaggc
    12361 aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc gtgccatatg
    12421 gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaacatccc ggggcttgag
    12481 ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt caaaaaagag
    12541 aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga agaaagcggc
    12601 gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat gtccacaagt
    12661 cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac tcccttctga
    12721 tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg cccaatgtat
    12781 gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa actggcagat
    12841 gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa atatcaggtg acggacaacc
    12901 atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt tatattcaca
    12961 cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg caagctttgc
    13021 aacctgcaaa ccttacgcct ggaacccatt agttttattg aaaaaagttt acgcgcttac
    13081 tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat ctactgcgtg
    13141 attgaacatc aaagctctgc agaaaagaat atggcttttc ggccaatgcg ctatgccact
    13201 gccgccatgc agcgtcacct agataaaggc tatgacagag ttccgctggt ggtgccattg
    13261 ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct ggatgagttt
    13321 gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt ggatatcacc
    13381 atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga actgattcaa
    13441 aagcatattc gcgaccacga tttaatcggc atggtcgaca ggatcaccac gcttttggtt
    13501 agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct gcaatgcggc
    13561 gatacctccc gtttcacccg ttttattcag gagattgccg aacgttcacc actacaaaag
    13621 gagagattaa tgactattgc tgaacggcta cggcaggaag ggcatcaaat tggctggcag
    13681 gaaggtatgc atgaacaagc cattaaaatt gctttgcgca tgctggaaca gggcattgat
    13741 cgtgaccagg tgctcgcggc cacccagcta agcgaagccg atctggcagc gaataaccac
    13801 taattaacac aggcccacag ccgatcccca tgggcctttg atatcaataa ctacctttta
    13861 tgcgtggccc atttttgcct gatttaaaca tcgccagtcg ttgatcaagg gcatcgctgt
    13921 agagcatcgt gtcaacgcca acagcgacaa agttcgctcc ccacgccagg cattgctgcg
    13981 ccatatcagg agccacagcc agaaaaccag ccgctttacc cgcagcacgg atccgccgaa
    14041 tgctggtttc aataattcgc tgcacttccg ggtgcccggc gttatccggg tagcccaacg
    14101 acgcagaaag atccgctggt ccaataaaca cgccatcaat cccttcgacg tcgaggatct
    14161 cgtccaggtt atccagtgcc gttttacttt ccacctgcac taacagacaa agcgaatcgt
    14221 tgacttgcgc catgtaattc tcaatgcgtc cccagcgcgc agcccgtgcc acactggccc
    14281 caacaccacg ctcaccgtag ggagggttgg acaaattttg ccggaggggg aatgtgccgg
    14341 tgcagtaaac cggcacagag tggcgaaatc agacggataa agtcatggac tcaacaacct
    14401 caatccagcc atgctcacta gcgatgtcct ggccgtttaa ccaacggcgt agcatattca
    14461 gtgccatcat tgcgcacact tcctgacgga tagccaggct gtagcgtgta gtgctgaaac
    14521 gcacacgcag agcgaaagtg ccgtctggcg tggctagcgc aaagttgaga tgctcgttct
    14581 cgaaacccga aacagccagt gccagcccgg caaaatggtt ggcccgccgt tctgtaatcc
    14641 agtgcgcagt ttgcgccagg gtttcttcct gtgaaggaac cacttcacac gccagcaatg
    14701 gagcacctgc gcgagaaagt tgcaaagcca ataaaccacc ggtgaattgc tcgctcaacg
    14761 tcaggctgaa ctggcggttt tgcaattcgc gactgatctg cgcgggcagt ccttcgttgc
    14821 cttcgaaaat cacgctctgt ccggcaacac gtttaacgtc cagccacagt ttttccatcg
    14881 cctgttgctc gcttgccggt ccggtgagtt tcagttcgat gatcggcatt gaggagcgat
    14941 agcccattgt tacgcccggc ggcagttgta gagtgtccag gctttgtgcc agatcgcttt
    15001 ccgaacgacc aaaggtagtt aaacgcagac aaaccggcgg ctgtggtaaa gaaaaacgct
    15061 cgcgcaggcg cggcaggatt tcgtgctcga ccatcacctt aaattctggc ggtacgccgg
    15121 gagtaaagaa catcaggcaa cgattaagct gcacggcaaa accacaggcg gtgcctaccg
    15181 ggttattgat aaattcagca ctggcaggca gctccgcttg tttacggttg ctcggtgcca
    15241 ttactcgtcc acgttcgtgg aaatagcgtt ccatctcttt gagccaggct tcatgcagca
    15301 ccaggccttc accttttgct gtcgcagcgg cgagtgcgct taaatcatcg ctggtcggtc
    15361 ccagcccgcc gttaacgatc agcacatcgg cgtgctgact acgttcgcga agaatggtga
    15421 ctaagtcatc aaggttatcc cccaccgtat tgcggcgaga taatggcagc ccctgatgaa
    15481 agaaaaaatc ggccagccag gcagcgttag tgtcaacgat ttgcccgtat aacacttcat
    15541 ccccggtgga tagcatttcc acttttaaca tggttatctc ctgcttttgt tatcgaatca
    15601 ctataacgca aagggagata aggcgtagta agaaactgaa tgccgcgccg gaagggatca
    15661 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    15721 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggac gcataatggt
    15781 gtaacgcgcg ccagcattcg cttcctcata actttgaata ccgctggaga gcgaatccgg
    15841 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    15901 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaca cgccttttcc
    15961 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgcccgcgac
    16021 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaaa gccaggcccg tcgattcagt
    16081 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tggccgctga tgctcattgc
    16141 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    16201 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    16261 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    16321 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    16381 aagtgcagtt atcattgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    16441 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    16501 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    16561 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    16621 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    16681 cgccgtggac cttcagcgat gatattcgca ccaagacata tgcagatggt cgcaaggaag
    16741 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    16801 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    16861 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    16921 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    16981 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    17041 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    17101 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    17161 gtttatctac cgtcagcttt ttaccctcgg aaaaccaggt cgccgactga atggtccgga
    17221 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    17281 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    17341 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    17401 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    17461 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    17521 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    17581 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    17641 cctacgatgt cggcaaaatg cctcaaattt ttgccaaatg caaagcctaa ataagaaaaa
    17701 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    17761 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    17821 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    17881 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    17941 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    18001 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    18061 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    18121 tttccttgct gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    18181 cgcctgaagc gatcgaatca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    18241 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    18301 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    18361 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gctgaaggtg
    18421 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    18481 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    18541 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    18601 tagtcaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag
    18661 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    18721 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    18781 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    18841 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    18901 aacgcatctg ttcgttgccg ctgttcccgg atatgactac cgccgatgcc gaccgcgtca
    18961 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    19021 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    19081 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    19141 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    19201 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    19261 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    19321 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    19381 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac cggcttattc
    19441 agcgcaccac tggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    19501 atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg attctggcga
    19561 atatcttcgc ccgccgtgca attgaaattc cagtacatca tgccgagcgt gagtttggtg
    19621 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    19681 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    19741 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    19801 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    19861 gattactcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    19921 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    19981 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    20041 tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg gttcggtggc
    20101 tcatctggcg gcggaaagag acattccggt ttatgcgccg gataacgtta atcatccgct
    20161 gtgggtggaa cgcattgccc aactgtcacc agaggtgatt ttctcttttt attatcgcca
    20221 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    20281 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    20341 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    20401 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    20461 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    20521 ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc cggatgacag
    20581 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    20641 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    20701 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    20761 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    20821 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    20881 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    20941 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    21001 ggatattggc agcgatgcga taagccgttt tctgaatcat ccacattttc actttgtcga
    21061 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    21121 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    21181 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    21241 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    21301 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    21361 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    21421 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    21481 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    21541 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    21601 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    21661 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    21721 aaaacatccg ctgcgccatc atttcccacc gtttgcggac tttcgcgttg tcgaaagtag
    21781 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    21841 ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    21901 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtaggctta
    21961 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc
    22021 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    22081 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    22141 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    22201 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    22261 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    22321 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    22381 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    22441 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    22501 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    22561 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    22621 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    22681 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    22741 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    22801 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggca
    22861 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    22921 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    22981 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    23041 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    23101 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    23161 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    23221 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    23281 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    23341 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    23401 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    23461 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    23521 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactagatg
    23581 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    23641 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    23701 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    23761 gcaaattgcc cacctatatt ctttcttgct ttgcacctct ggcaatgctg ttggcgcatt
    23821 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    23881 tcgcttttgg cgtcactggc attattgcca catttgtgat ctccccctgg ggaccaatga
    23941 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    24001 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtctttcg
    24061 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    24121 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    24181 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    24241 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    24301 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    24361 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    24421 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    24481 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    24541 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    24601 gtggctggga ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc tgctggtctt
    24661 gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg tctgggtgac
    24721 gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc
    24781 gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct gatatgggga
    24841 ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc ggcaagtcat
    24901 ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt tggcctggat
    24961 gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg ttggtataaa
    25021 acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg
    25081 gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct gaaagcacta
    25141 ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac gacaaaacaa
    25201 cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg gctataactg
    25261 cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata aagcgcattc
    25321 tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac ttcggcgatc
    25381 atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc ttgtttattg
    25441 cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa gagttttcac
    25501 atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg cagaaatgag
    25561 aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg
    25621 aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc accggttgtt
    25681 gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc tggtcatact
    25741 ccatcaccgc taccggccga tgaccaaact ccttgtcggc aacgggaacg ataaacacct
    25801 gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga ataccctcac
    25861 cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
    25921 caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt
    25981 tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt
    26041 tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct tctttcgcac
    26101 ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc
    26161 gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg
    26221 aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag
    26281 tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag
    26341 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
    26401 accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca ctggcaagat
    26461 gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag gtcaacgtca
    26521 ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc agctgaatgt
    26581 gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg
    26641 tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca
    26701 cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc gtattccacg
    26761 cccgcaacat cacgccgctg ccctcaacca caccctgcgc cgcaaagccc gtagctaact
    26821 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
    26881 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa
    26941 ctgctccagt acatcagcgt ccactaacgg cagtgggctc cccggccagc gacgaacctg
    27001 ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg ttaaccaggc
    27061 agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac tgatcaccgc
    27121 cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca gactgcccgt
    27181 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg
    27241 ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga
    27301 atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatcagggtt
    27361 aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg
    27421 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc
    27481 ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg caagtttgag
    27541 gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg ctgcttgcgg
    27601 caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg aaggcatctg
    27661 cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca cgctttgcgc
    27721 ctcttcccag gtttcctggc tgaagcccgg cagtggggag atctcccccc acccttcgcg
    27781 ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    27841 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    27901 ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt gcgaccttcc
    27961 tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagcccc
    28021 gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc catcgggctg
    28081 ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc cagcggtacc
    28141 acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac
    28201 cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg
    28261 ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgctgcgata
    28321 gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc agccaccatc
    28381 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    28441 tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg
    28501 gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt gtcgtcataa
    28561 cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    28621 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    28681 tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat tgcttcatca
    28741 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    28801 cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    28861 cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    28921 acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg
    28981 cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc accagctccc
    29041 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg
    29101 ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    29161 cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc ccacaaagcc
    29221 ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag taccccacca
    29281 gccagaagtc aaggatattg taactaacca aggttttacg cagtaagtcg gtgacatcat
    29341 caaatccatc gacgctaatc gccgccgaac caccatgacc tgggagatca acatacaacc
    29401 gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg ccggaaaaac
    29461 cgtgcaaaaa caccagccag ggtaaacctg gttttccgtg ttttgcctgc gcgtgcagga
    29521 tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt
    29581 aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa atgccgtttc
    29641 aagttcctgc cagttttgcg gacgatgata tttcagctcg aacatcgcgg cggcgtgctc
    29701 aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg gcgttggcaa
    29761 cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcagaaac
    29821 ctgacgcaat aacgccagcg cgttgagatc gtaaagtgcg gagagatcgc ccacaatcgc
    29881 cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca gtccgtcgat
    29941 accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    30001 aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag gcagatagtc
    30061 gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa taaccgcctg
    30121 cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    30181 atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat ggtgtgccgg
    30241 atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac agcttgcctg
    30301 ccattgcagg agccgtttcc ccgtcaggca gcttcccagt tgcaccacaa tttgcgcctg
    30361 ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg gcagcggctg
    30421 cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg cccacagggc
    30481 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgtcg
    30541 ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac gcagccacgg
    30601 tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg tatcgtccat
    30661 ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg catgaagcgt
    30721 accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    30781 cggcaatgaa atactgtgcg tggggtgaga gccgaacatt cccggctggc gaattgcctg
    30841 attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa tcagtttttc
    30901 tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg tgccggaggt
    30961 cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccaa
    31021 cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    31081 taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt gacgcgttaa
    31141 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    31201 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    31261 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    31321 tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat
    31381 ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag aattcgcttt
    31441 gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac
    31501 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    31561 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    31621 gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat
    31681 cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat
    31741 catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta
    31801 ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    31861 gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattca
    31921 gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt gcgaaatgca
    31981 ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg gttttcgttg
    32041 ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg
    32101 ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tcttttgcct
    32161 gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac
    32221 cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac gggtcaaaag
    32281 cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    32341 cctggtccaa cgacgtaaaa cgggtaatcg cgcccagggc ggcagcttct tcatcaccat
    32401 tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat
    32461 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    32521 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    32581 cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg
    32641 gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg acggccgcgc
    32701 ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct
    32761 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    32821 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    32881 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat
    32941 caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga
    33001 caagagttgt tgattacgcc tgaattacat cgcgtgccat cccaatgtgt gggataccat
    33061 cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat
    33121 gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat gtttcgagtg
    33181 ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg acaatcaccc
    33241 gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca tacgccacca
    33301 gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga
    33361 tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg
    33421 cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc
    33481 tctcctttta gatgtatgcc tt
//
LOCUS       DADKXM010000032        31525 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.032, whole genome shotgun sequence.
ACCESSION   DADKXM010000032 DADKXM010000000
VERSION     DADKXM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.032"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..2079)
                     /locus_tag="JQR05_001484"
     CDS             complement(<1..2079)
                     /locus_tag="JQR05_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY5323683.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKSENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLEPLATLAWTL
                     GFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRK
                     IADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREI
                     DPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGIL
                     ENAFWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANA
                     KPQCEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDI
                     NLDNQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVW
                     NMLNYAALQEG"
     gene            complement(2097..4073)
                     /gene="mngA"
                     /locus_tag="JQR05_001485"
                     /pseudo
     CDS             complement(2097..4073)
                     /gene="mngA"
                     /locus_tag="JQR05_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            4183..4905
                     /gene="mngR"
                     /locus_tag="JQR05_001486"
     CDS             4183..4905
                     /gene="mngR"
                     /locus_tag="JQR05_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY5323684.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5009..5878)
                     /gene="sucD"
                     /locus_tag="JQR05_001487"
     CDS             complement(5009..5878)
                     /gene="sucD"
                     /locus_tag="JQR05_001487"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY5323685.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPEGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(5878..7044)
                     /gene="sucC"
                     /locus_tag="JQR05_001488"
     CDS             complement(5878..7044)
                     /gene="sucC"
                     /locus_tag="JQR05_001488"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY5323686.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7138..8355)
                     /gene="odhB"
                     /locus_tag="JQR05_001489"
     CDS             complement(7138..8355)
                     /gene="odhB"
                     /locus_tag="JQR05_001489"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY5323687.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(8370..11171)
                     /gene="sucA"
                     /locus_tag="JQR05_001490"
     CDS             complement(8370..11171)
                     /gene="sucA"
                     /locus_tag="JQR05_001490"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY5323688.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDTNWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(11472..12188)
                     /locus_tag="JQR05_001491"
     CDS             complement(11472..12188)
                     /locus_tag="JQR05_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY5323689.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12204..13970)
                     /gene="sdhA"
                     /locus_tag="JQR05_001492"
     CDS             complement(12204..13970)
                     /gene="sdhA"
                     /locus_tag="JQR05_001492"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY5323690.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDTVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(13970..14317)
                     /gene="sdhD"
                     /locus_tag="JQR05_001493"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(13970..14317)
                     /gene="sdhD"
                     /locus_tag="JQR05_001493"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY5323691.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(14311..14715)
                     /gene="sdhC"
                     /locus_tag="JQR05_001494"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(14311..14715)
                     /gene="sdhC"
                     /locus_tag="JQR05_001494"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY5323692.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15096..15203
                     /locus_tag="JQR05_001495"
     CDS             15096..15203
                     /locus_tag="JQR05_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323693.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            15409..16692
                     /gene="gltA"
                     /locus_tag="JQR05_001496"
     CDS             15409..16692
                     /gene="gltA"
                     /locus_tag="JQR05_001496"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY5323694.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            17082..17648
                     /locus_tag="JQR05_001497"
     CDS             17082..17648
                     /locus_tag="JQR05_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5323695.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTVEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            17699..20158
                     /locus_tag="JQR05_001498"
     CDS             17699..20158
                     /locus_tag="JQR05_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5323696.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     IDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSDK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKVTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            20173..20904
                     /locus_tag="JQR05_001499"
                     /pseudo
     CDS             20173..20904
                     /locus_tag="JQR05_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            20901..21962
                     /locus_tag="JQR05_001500"
     CDS             20901..21962
                     /locus_tag="JQR05_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5323697.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            22069..23160
                     /locus_tag="JQR05_001501"
     CDS             22069..23160
                     /locus_tag="JQR05_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY5323698.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(23157..23948)
                     /gene="nei"
                     /locus_tag="JQR05_001502"
     CDS             complement(23157..23948)
                     /gene="nei"
                     /locus_tag="JQR05_001502"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY5323699.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKKRLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(23984..24718)
                     /gene="pxpA"
                     /locus_tag="JQR05_001503"
     CDS             complement(23984..24718)
                     /gene="pxpA"
                     /locus_tag="JQR05_001503"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY5323700.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(24708..25640)
                     /gene="pxpC"
                     /locus_tag="JQR05_001504"
     CDS             complement(24708..25640)
                     /gene="pxpC"
                     /locus_tag="JQR05_001504"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY5323701.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLFHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(25634..26290)
                     /gene="pxpB"
                     /locus_tag="JQR05_001505"
     CDS             complement(25634..26290)
                     /gene="pxpB"
                     /locus_tag="JQR05_001505"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY5323702.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDSARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(26313..27056)
                     /gene="ybgI"
                     /locus_tag="JQR05_001506"
     CDS             complement(26313..27056)
                     /gene="ybgI"
                     /locus_tag="JQR05_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY5323703.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            27327..28808
                     /gene="dtpD"
                     /locus_tag="JQR05_001507"
     CDS             27327..28808
                     /gene="dtpD"
                     /locus_tag="JQR05_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY5323704.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(28958..30376)
                     /gene="phrB"
                     /locus_tag="JQR05_001508"
     CDS             complement(28958..30376)
                     /gene="phrB"
                     /locus_tag="JQR05_001508"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY5323705.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARIQTLAAYEAARKGK"
     gene            complement(30373..30882)
                     /locus_tag="JQR05_001509"
     CDS             complement(30373..30882)
                     /locus_tag="JQR05_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY5323706.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            31011..31340
                     /locus_tag="JQR05_001510"
     CDS             31011..31340
                     /locus_tag="JQR05_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY5323707.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQELREELYQVKQVVKGMRDGM
                     FSVLNES"
BASE COUNT         7582 a         8495 c         7756 g         7692 t
ORIGIN      
        1 cccttcctgt aaggctgcat agttgagcat attccatacc ggaaccggcg cttctttcca
       61 gccttcttgc tgccagttgt tcattgcact gtcgttcacg gggcgcgtta gcgaaccaaa
      121 ctgggtatct gccagaacac tatcggtgtt aaaagatgta gggatcagga cgcgaaggcg
      181 atgatcgtca gcctggttat caaggttgat atccacatca atgcgcctgt tgttatgact
      241 aagagtgaca accaactcga cacctactct gccagtggat tgtcttgcgc tgcgttgttg
      301 cagattacgc ggcaccgcca tttcatagcg gataacagcc ctgctctgcc aggcctcatg
      361 aataatctcg cattgcggtt tcgcattcgc cgaagtaatt acccactctt cttttgcggg
      421 tgaatagtca tattcatcac catcatcaga gctttcttca atttgcaaca cccggtcata
      481 gcgcacaccg ctatctttat ctaccagttg tagcgttcca tcctcattga gcgcaatttg
      541 ccagaaagca ttttccagta tcccttcagc gtcacttttt gccgcaatca cgttgccagg
      601 ctgattcgct tcgatataaa gcgtgcgata gcccatagaa gggacaatct ggttgatctg
      661 tatatcaaac tccataaagg gatcgtaatt accgtaatga actatttgcc gatcgattag
      721 gcctggatcg atctcacggg catggcgaat aaaatacggt acaggctgac cgcgatcgtc
      781 tcgtagatta aactggctgg cgcgcagccg cacagtggtg ttgatcactt cttcccgcgg
      841 ccagggcatc aggttaaaca ggacgagttt gtcggcgtcg ctctgcggca tgttgtcggc
      901 gattttacgc atatagaaac gcagcagatt atccgccatg tcttcagcca gttcgaagcg
      961 ggcgacgatt tcgcgatgaa ctttgtcact acagcagcag ccgatgctgt cgtgggcatg
     1021 attttttaag atctctttcc acattttctc cagcaagccg tggtggtatt caaaacccaa
     1081 cgtccaggcc agcgttgcca gcggttccag cggttccagc ggttccagcg gttccagcgg
     1141 ttccagcggt tccagcggtt ccagcagatt aacaatctta ttttcaatac gtgcgtgggc
     1201 aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat atttgccatc
     1261 aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct tctcaaatac
     1321 ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca gtttctccat
     1381 cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca gcaaaatctc
     1441 tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt tgcgtaatcc
     1501 gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca cctgcgccgt
     1561 cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac cgtggcgctc
     1621 agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga tatgcggcag
     1681 ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct cgccaaacgc
     1741 gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag aaacaatcgt
     1801 ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta ccagtttctt
     1861 cacgcggtct ttgttttccg atttcaccgc gaaataatct tcgaggatcg ccgtttgccc
     1921 gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga tctcttccat
     1981 attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc gatcccagtg
     2041 catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct ttttgtttat
     2101 ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg ccagatcagg
     2161 agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc aattgccgcc
     2221 ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc agcaccgggt
     2281 gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga gcccagcaca
     2341 aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc ttcggtaata
     2401 cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc tttaaacagg
     2461 cgcggtgcga gcatcgtgga agcagttacg gtaaatgccg aaaccatttt gacggaggcg
     2521 aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaagcagaa tgcataagcg
     2581 gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat ggcacccagc
     2641 aacagcgcgt tacttcctga cagaccgttc agctaggcgg taagcgagtt attgatccag
     2701 gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt accgagcacc
     2761 gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa gtgatttttc
     2821 atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag aaacccggag
     2881 ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg tttatctgcc
     2941 agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag cagcccgccg
     3001 cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc ttgtagcccg
     3061 aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac aatcaacggt
     3121 acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac gcttttcacc
     3181 ggttgcgtat cttgctgtac ggtacgcgtc tcatcgccac gctcgagggt aagcgattgt
     3241 tggatcaacg cttctgcatg gcgaatcggc tcggcaacag acacagaaag cgcaggaatg
     3301 ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca ggcagttgca
     3361 ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg cttttcaacg
     3421 tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc catgtaggtg
     3481 tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt ggaaaaagag
     3541 gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc aggcgtcgtc
     3601 gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag cgctgtcagc
     3661 agttgcatgt gcgtagtacc cgcttcattg gggggaatcg ccagcaacac cactaaatca
     3721 accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt ggcgaccgca
     3781 aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc ttcacctaag
     3841 gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc agtactggaa
     3901 attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc acggctggta
     3961 aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcagatt catcatgatc
     4021 ctcacttgat cgtttttata gtcgcgccag tgtgcccgat aaaacaatac cattgcgtga
     4081 tcaatttcac ttgattgaaa ttatttacat tgtatttgta ttatttacag gatgaattta
     4141 tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca caaaccctta
     4201 taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct taaacctggc
     4261 gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg ggttacggtg
     4321 cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca gggcagcggg
     4381 acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag ttttgatgaa
     4441 aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga agtgattccg
     4501 gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt gtggcatgtg
     4561 aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg gatgccgctt
     4621 gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca ctttattgaa
     4681 gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct gatgccaacc
     4741 gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga aaaagtatcg
     4801 cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt taataccgat
     4861 gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca aaaaaggcca
     4921 tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt agagggaagc
     4981 ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt gcttcaccga
     5041 tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg aatttctcat
     5101 ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg gcccatacgt ttgccttccg
     5161 gcgcagtcac accagcgatg taacccacaa ctggcttggt aacatgctct ttgatgtacg
     5221 ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc gcttcggtct
     5281 gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt agagccaggg atcgggtcac
     5341 cgccgatacc gacacaggtc gactgaccga aaccgtaatc cgtggtctgt ttaaccgctt
     5401 cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg tgaatgtgac
     5461 ccggctggat accgattttg cattcacccg gggtgataac gcctgggcag ttcgggccga
     5521 tcatacgaac gcctgcttcg tccagtttca ctttcacggt cagcatatcc agcgtcggga
     5581 tgccttcagt gatggtgata atcagtttga tgcctgcatc gatagcttcc agaatggagt
     5641 ctttgcagaa cggtgctggt acgtaaataa cagacgcgga agcgccagta gcagcaacag
     5701 cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtgccgcct ttacctggag
     5761 ttacgccacc aaccatttta gtgccgtatg caatggcctg ttctgagtgg aaagtcccct
     5821 ggctaccggt aaagccctgg cagataacct tggtgttttt atcaattaaa atggacatta
     5881 tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt ttgctgcaat
     5941 aatattcagg ccgctgtcag ccagtttctt cgcgccgagt tcggcgttgt taccttccag
     6001 acgtaccacg accggtacgt taacacccac ttccgctacc gcgccgatga taccgtcagc
     6061 gatcaggtcg caacgaacga taccgccgaa gatgttaacc agaacggctt tcactttgtc
     6121 gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc cgccgccaac
     6181 gtccaggaag ttagccggtt cgccgccgtg cagtttaact atgtccatcg tacccatcgc
     6241 cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt agttcagttc
     6301 ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca tttcgcgcag
     6361 atcaggctgg cggaacaggg cgttgccatc agcgcccagt ttgccgtcga ggcaaatcag
     6421 atcgccctgt ttggtgatga ccagcgggtt gatttcgatc agcgccaggt cgcgctccag
     6481 gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt taccttccag
     6541 acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca gcggatcaag
     6601 cgcaacttta tggatcaggt gcggagtttc ttccgccact ttttcgattt ccacgccgcc
     6661 ttcggtggag gccataaaga ccacgcgacg ggaactacgg tctacaacgg caccgagata
     6721 cagctcttta gcgatatcgg tcgccgcttc aaccagaatc tggttaaccg gttggccatt
     6781 ggcatctgtt tgatacgtta ccagacgctt gcccagccag ttttctgcaa aagcacggat
     6841 gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac cagcgtgaac
     6901 ctgacatttc actacccacg gaccggcacc gatttttgaa gcggcttctt ctgcttcgcg
     6961 cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa acagttgttt
     7021 tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct ccagtaatcg ttatctttta
     7081 aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct taaactacta
     7141 cacgtccagc agcagacgcg tcggatcttc cagcaactct ttgatcgtta ccaggaagcc
     7201 cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt acatcatcgg
     7261 caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt gcatacccag
     7321 aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga acacaccacc
     7381 gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc catcacggcc
     7441 tttgactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg tatcgacatc
     7501 acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc gccatgctga cgtcgaaata
     7561 gttgtggtaa accacgtcat cgccgtcgat agacgcgttc acttccgggt aacgtttcag
     7621 ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc cgtggcgttt
     7681 ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt tgacttcgtt
     7741 gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg ccacacgctt
     7801 acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg gttgcgccgc
     7861 cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat gtttttccac
     7921 atcttcacga gtcagacgac cacccacacc ggtgccttta atggcgctgg cgtcgagatt
     7981 gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt gctcttccag
     8041 agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg cgctggtttc
     8101 tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg ttaccgttgt
     8161 accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta cttccagtac
     8221 cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt cgccgggttt
     8281 tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag ggaccagaat
     8341 atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc attaaccaga
     8401 tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc ggaggccggg
     8461 cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg atgctggctg
     8521 cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc cttgacgtga
     8581 gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag ttgctcgata
     8641 cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag gtcgtaataa
     8701 accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagctc gtcgatttca
     8761 ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac cgccagcgga
     8821 tgacgcagca gggatttcgg cgacatcacg accagcggac gacgcatccc gcgcagcgcc
     8881 tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gtacgcaaac ctgcatgttt
     8941 tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc cggcccctgc
     9001 ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca tttctgctcg
     9061 ccagaggaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc accgaactgc
     9121 gcttcccaga tggtcagggt gcgtggttct gcggtggcat aaccatattc aaacgccagc
     9181 acggcttctt ctgacagtac ggagtcccag acacggaacg cgccctgacc gttatggatg
     9241 tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc gtggcggtgg
     9301 aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc ttcatcaacc
     9361 agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc accggctgcc
     9421 atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac tgcttctggt
     9481 accgtactga tacgtttagc cagttcctgc aggcgcttca tctcaacttt gttcgggtac
     9541 tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat gttcatcgga
     9601 cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag gttaaccatc
     9661 tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc gtagattttg
     9721 cgcggtgtcg gatgtttttt gattttctga tacatcagtg gctgggttgc gctcggctcg
     9781 tcggcttcgt tgtggccgtg acggcggtag cataccaggt cgatgaagac atcacgttta
     9841 aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc cggatcatcc
     9901 gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca gtacggtgta
     9961 gaacgggcat ccagcgggtt agaggtggtg aaaccaacct ggttgttgat aacgatacgt
    10021 accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc ctgaaccacg
    10081 ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt gttgctgctc
    10141 ggctcatcaa gtctgtccag acgggcacga acagagccga taacgaccgg gcttacaatc
    10201 tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc ggtctggaag
    10261 tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc cgaggtgttc tttatgttta
    10321 ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac cagcacgttc
    10381 agacgaccac ggtgcgccat cccgagaacc acttcgcggg tgccgctgtt gccagcgtgg
    10441 cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga gaagcgtttt
    10501 gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt cagcggcggt cagttcgctt
    10561 aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat acgctgttgg
    10621 atccagcgtt tttcttcggt actggtgatg tgcatatact cggcaccaat cgggccgcag
    10681 taggtttgct tgagggcgga aataagctcg ccgagtttca tggtttcttt gccgctggca
    10741 aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg tcagatcgtg gaaagacgga
    10801 tccagatcgg ccactttatc ttgctgccac agtcccagcg gatctagatt cgcatgctgg
    10861 tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt cacattggtg
    10921 tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg gcggaaatat
    10981 tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa ctgctggaac
    11041 gtcgaacgcc agttagtgtc aaccgagtca ggatcggtta agaagtcttc atagagctgt
    11101 tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc tttcaaagcg
    11161 ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg tggatactac
    11221 cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctacg tagtggctcg
    11281 cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat ttcctgcatt
    11341 tctttgttac gcctgatgcg ctgcgcttat caggcctgcg ggtaatgact tgtaggccgg
    11401 ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg caacgcttgc gcgtcttatc
    11461 aggcctacag tttacgcatt acgttgcaac aacatcgact tgatatggcc gatggcgcgc
    11521 gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg acagcggaat
    11581 acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc acggctatcg
    11641 atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc gggattccac
    11701 cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata cagcccgtcg
    11761 agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt ttgtccatta
    11821 ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat gtctaccacc
    11881 aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt gcccggctgg
    11941 ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat gttcagaccg
    12001 tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg atctttctct
    12061 ttcagctgga taagcgcatc cagcagcatc atgtcacgac cttcttccgc ttccagggtg
    12121 taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat tgaaaactcg
    12181 agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa tgccgggcgc
    12241 agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactcagg cagatacagg
    12301 gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct atgtgcgcca
    12361 cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc catcaggtta
    12421 tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc atccagacgg
    12481 gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc catcgcatca
    12541 ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt acggatcgcc
    12601 accgtatctt caccgttacg attattgttc cagcggttca ggcgatccag agacgcttca
    12661 acatcagact cgctggcatc gcgcagtgcg ccctgctcgg cgatagactc ttgcagatgc
    12721 agacctgccg cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc cagacggtta
    12781 gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagtcccgg aacaaccaca
    12841 tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa ctttggtcgg aataccgccc
    12901 atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc gacgtgagcg
    12961 aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc tttacccagg
    13021 tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg accttcacgg
    13081 atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt ggcgttcggc
    13141 gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc ttcaccacgg
    13201 caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa ttgccacatt
    13261 tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc gccagtgtta
    13321 atgtgggcgt tagtggtgga ctgataaata cgccctgccc cgccagtcgc cagcacggta
    13381 gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt acaacccacc
    13441 accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa aatggtggtg
    13501 tggtttttca ggttctgctg ataaagcgtg tgcaacagtg catgaccggt acggtcagca
    13561 gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc gccaaacgga
    13621 cgttgataga tacgaccatc atcgagacgc gagaacggca ggcccatgtg ttcgagttcc
    13681 agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc accgatatag
    13741 tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg ggtattaccc
    13801 agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg gaagacttta
    13861 gagagcagcg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg catacctgcg
    13921 ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat cacacacccc
    13981 acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc accagttgca
    14041 gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa cacctgccac atgccgatcc
    14101 aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg aacgcagagg
    14161 cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa aaaccgacca
    14221 tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg aaatcatgta
    14281 cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg cgagaagtga
    14341 aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt cgaatgtttc
    14401 ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt gatacgccag
    14461 agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa tcgcggaagc
    14521 ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccacagca ggatgcccac
    14581 tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg ccgtgatggg
    14641 gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat ttcttatcat
    14701 gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca gaggttaact
    14761 ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcagg tcagacagcg
    14821 tcctttctat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa cgacacgtta
    14881 caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggcag tataggctgt
    14941 tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct gaacagtgat
    15001 ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca atcattcaac
    15061 aaaattgtta caaacattac taggaaaagc atataatgcg taaaagttat gaagtcggta
    15121 tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca ttcgggacag
    15181 ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc gccatttatt
    15241 cgtcatcaat ggattcttta cctgcaagcg cccagagctc tgtacccagg ttttcccctc
    15301 tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt gcgaaggcaa
    15361 atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat ggctgataca
    15421 aaagcaaaac tcaccctcaa cggggacaca gctgttgaac tggatgtgct gaaaggcacg
    15481 ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt cacctttgac
    15541 ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga tggtgatgaa
    15601 ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc taactacctg
    15661 gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta tgacgaattt
    15721 aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct gttccacgct
    15781 ttccgtcgcg actcacatcc aatggcagtc atgtgtggta ttaccggcgc gctggcggcg
    15841 ttctatcacg actcgctgga tgttaacaat cctcgtcatc gtgaaattgc cgcgttccgc
    15901 ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat tggtcagcca
    15961 tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat gatgttctcc
    16021 acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga ccgtattctg
    16081 atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac cgctggctct
    16141 tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg gggacctgcg
    16201 cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc cgttaaacac
    16261 attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct gatgggcttc
    16321 ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga aacctgccat
    16381 gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat ggagctggaa
    16441 aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa cgtcgatttc
    16501 tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac cgtcattttc
    16561 gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag tgacggtatg
    16621 aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgattt taaaagcgat
    16681 atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgctgt tcaaatggcg
    16741 ggttaatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccac tatcggacat
    16801 tcatatattc taaaaaaaat atatcggtat aaattataaa caaatcgatg atttttaatc
    16861 atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc ctataatttc
    16921 caatacttta ttcatcaaac atttaattca taccatcaat taattacttt tacatttaat
    16981 aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata agatattctt
    17041 gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa ggacaaaaaa
    17101 cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg gctgatgaag
    17161 gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg
    17221 atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcac
    17281 atcacagcga tgccgttgct gtcgacctgc gcttagtcaa ctgtgatctg gaaaactcca
    17341 gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa
    17401 ccaccggcgc agatccgatt ctcaacaaca ccagcacggt tgaagccacc ggcgtcggcg
    17461 tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg
    17521 acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg gaacaaattg
    17581 atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt
    17641 ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt tatcaggtat
    17701 ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg
    17761 cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg accgcattga
    17821 tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg ttagcgttgc
    17881 ggtaaataat aaccaaatca gtaacgggca aaagattaac tggcacaaaa atgacgataa
    17941 aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac ctgaagtccg
    18001 tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg aaatgctctt
    18061 caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct ggctggcgtg
    18121 gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg gtgtcctgat
    18181 agattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca gcactaatct
    18241 taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca gtgattatca
    18301 gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat cacgtaccta
    18361 tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa cggattttag
    18421 ttccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg acgatcgaat
    18481 gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac agaccaatgc
    18541 cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc caccaggccc
    18601 atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca aagtgacgga
    18661 agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct tcctgactcg
    18721 tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca tgtcacatca
    18781 aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaacacctc
    18841 gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa tgggtattgg
    18901 gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt
    18961 tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca aacaagtgga
    19021 tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc attttcacag
    19081 ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg aaaaacagac
    19141 gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt acgccaacct
    19201 gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca cggcaggttt
    19261 taatgttgat attggtgact ggagagatat ctctatttcg acgtcattca atacaaccca
    19321 ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc ccttcggtaa
    19381 cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac accgcatgtc
    19441 gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac tgcaatccga
    19501 ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt cagcgggtga
    19561 gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca gcagctggag
    19621 cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct ccaccaatga
    19681 accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg gcaatctcga
    19741 ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc agccttccac
    19801 cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa acgttatcaa
    19861 agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt ccgataaaga
    19921 cgttaacgtc atcattcgca acgccagcgg ccagtttcct cccctcggag cggatatccg
    19981 ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg cctggttaag
    20041 cggagtcgct gaaaatcaaa aggttaccgt ggtctggggt gatagccagc attgctcgct
    20101 ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt gtcattaatt
    20161 aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt tgccagtctg
    20221 tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc taatgataaa
    20281 gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc ttattcctag
    20341 ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc gccgatacaa
    20401 cgtctggaac cgaaagcaac aacgcaggta cggatagtaa agcaagcctc gaccacaaaa
    20461 ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc tcccgccccg
    20521 gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat caaggtgttc
    20581 tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca gttacaggtc
    20641 agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct gaccattgcc
    20701 tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat ggttgcgcct
    20761 tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta tctcggttat
    20821 atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca atgccattta
    20881 caagccgtgg agacgaagaa atgagtgctg gcaagggagt gttgctcgtc atctgtctgt
    20941 tatttctgcc gttgaagtct gcgcttgccc tgaactgcta ctttggttca tccggtggct
    21001 cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc aaactcggcg
    21061 ataagatctg ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac aataccaacg
    21121 gcaactttga aagcgaacat gtctacgcat gggtaaaccc ctacccgggg gtgcaggatc
    21181 gttattatca actcggagta acttacaatg gcgttgatta tgacgccaac cagggaaaaa
    21241 gccgcatcga cactaaccag tgtatcgaca gtaagaacat tgacatttat acccctgagc
    21301 agatcatcgc gatgggatgg cagaacaaaa tttgctcggg tgatcccgct aatattcaca
    21361 tgtcgcgtac ctttctcgct cgcatgcggt tatacgtcaa aatacgagaa atgccgccgc
    21421 atgattatca aagtacgctt agcgactata tcgtcgtgca atttgacggt gccggtagcg
    21481 ttaacgaaga ccctactgcc caaaacctga aatatcatat tactggtctg gaaaacattc
    21541 gcgtgctgga ttgcagcgtc aatttttcca tttcaccgga aacacaggtg attgattttg
    21601 gtaaatttaa tttgctggat attcgtcgcc acacaatgtc gaaaacattc agtattaaga
    21661 cgacaaaatc acaaaatgac caatgcaccg acggatttaa agtcagttcc tccttttata
    21721 ccgaagaaac gttggttgaa gaagataaag cattgttgat tggtaatggt ctgaagctac
    21781 gtttgctgga tgaaaacgcc tcgccttata ccttcaataa atatgctgaa tacgccgatt
    21841 tcaccagcga catgttggtc tacgaaaaaa cctatacagc tgaactttcg tccattgcag
    21901 gtacccccat cgaggctggg cccttcgata cagtggtgct ttttaaaatt aactataact
    21961 gatttgccat gatgcccttt gcagccggaa agggcatctc tttcagaaaa ttgctgattt
    22021 tccccttttc cttgattgct gttatttttt cctagcccgg ctttctttat gctgtatcga
    22081 tacaccaaag agaaaataat gagggagcga aggatgccag ttttgcagtg gggaatgtta
    22141 tgtgtgctat cacttctcct ttctattggt ttcctcgcgg tacatctccc ggcggcgcta
    22201 ttacttgggc cgatgatcgc cgggatcatc ttcagtatgc gcggaattac tctgcaactt
    22261 ccccgctccg cttttctcgc cgcgcaggcc attcttggct gcatgattgc gcaaaacctc
    22321 actggttcga ttctcaccac gctggccgcc aactggccga tcgtgcttgc tattttactg
    22381 gtgacgctgc tttccagcgc catcgtgggt tggttattgg tgcgctatag ctcactgcct
    22441 ggaaataccg gtgcctgggg ttcttctcca ggcggcgcag cggcaatggt cgctatggcg
    22501 caagattacg gcgcagatat tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc
    22561 gtcgcggggg ccgctgttct ggttacccgc atgatgctgg gtgataacgc tgaagcggtt
    22621 aaccagcaaa tcgtctggtt cccgcctgtg agcattaatc tcctgcttac gattttgctc
    22681 gcagtcgttg ccggtacggc gggatgcatg ctgcgtcttc cttccggcac gatgctcatc
    22741 ccgatgctgg cgggcgcagt gctccagtct ggtcagctca tcaccatcga actaccggaa
    22801 tggctgctgg cgatggcgta tatggcaatt ggctggcgga ttggtcttgg tttcgataaa
    22861 caaatcttac tgcgggcact acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg
    22921 ctggctattt gtgcaggtat ggcgtgggga ctgacccggt ttatgcatat tgattttatg
    22981 actgcctacc tcgccaccag tcccggcggg cttgataccg tggcggtcat cgccgcaggg
    23041 agcaatgccg atatggcgct tattatggcg atgcaaaccc tgcgcctgtt cagtattttg
    23101 ctgacggggc cagccattgc gcggtttatt tcaacctatg cgccgaagcg gtcggcctag
    23161 tgctggcagc ctggacacca gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg
    23221 ccgccacagc gttcgcacag ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc
    23281 ccatgatgct tattctcatc cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc
    23341 agtaacgcgt gtgcgagtgc atccagttgc gccgcattga gatctttcgc tttatgattt
    23401 ccagtcaacc caacctgcca gaggatctcc acccgcaaat aattgccaag cccagccaga
    23461 aacgcctgat cgagcagtaa tcctgcaaac tgacggttac gaaagcgcgg cgacaataac
    23521 cgtttcttta ccacctccgg cgtcagattc ggatccagca catcgggacc aacgcgttgt
    23581 aaaaacggat gcgtggtcag ttgttccggc ctcaacatct caatatccga ggcgctataa
    23641 agcagaatgg ttttgtcagc cgtttgcagt tttacccgca atactcgcgt ggtctgcggc
    23701 tcttcgccgg tatcaaccac gcgccagacg ccgtaaagct gattatggct gtagagcgtt
    23761 aaaccgttgg gaaaatgagt taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt
    23821 tgaccgataa gttgtgattg ataagttttt aactgcggga aggcaaacca gacatcagtt
    23881 agtggtttgc ctttgatcgc cgcctccagg ttatccgctg cacggcggat ctccgggcct
    23941 tcaggcatgg tgatatcctt ttttcattct ttattaagcg gggttatgct gcgacaacaa
    24001 tccccttttt ggcaaatgta gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt
    24061 cgccatgcag gcagaccgtt tgcgccgcga ccgttgccca ttcgccggtg atacttttga
    24121 ctctgccgtg ttgcaccatc tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca
    24181 acgcgcctgg ctgacttcgc ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa
    24241 acacttcctc gcgcgttgtc aggccatatt gcttgcctgc acgaatcagc tcgcttcctg
    24301 ccagcccaac gagaaccaat gccggatcgc aagcgtatac cgctctggcg atggcgtctg
    24361 ccagttgtgc ttctttcgcc gcctggttgt acaacatgcc gtgcggtttg acatgacgca
    24421 ttacgccgcc ttgcgcacgg gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg
    24481 cgtaaacggt ttccggcggc agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa
    24541 aactcgggtg agcgccaatc gcgacaccat tttttattgc ttcacgcacg caagcctgca
    24601 tggtttgcgc atcgcctgca tgaaatccac aagcaatatt ggcagaggaa accagcgtta
    24661 atagctctgc gtcgctggcg cagccttcgc ccagatcggc gttcaggtca attttcattg
    24721 tgcagccgcc acgctaattg ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc
    24781 tcttccagtg aacactggac aaaatgaatc ggctgaccga gcggaatttg cgccagatgg
    24841 tacatatcag cctcaatgat acaggcaata cgtggataac caccggtggt ctgtgcgtcg
    24901 ttcatcaaca caatcggctg cccgttatgc ggcacctgca ccacgcccgg taacaaaccg
    24961 tgaaataaca gttcgcgatc ggtgatgcgt tttaaaattt gcccctgtaa gcgatagccc
    25021 attcggttac tttgcgagct aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg
    25081 cgatcgaact catgatattc cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc
    25141 tgtttaacgc cctgcgcttc cataaaatca cgcttcgatt tgccaatcgg gagtcggtca
    25201 ccatccttca gtaaacggcc ttccagcccg ccaatcccca ctttgagatc ggtgctgcat
    25261 gaccccatta ccggcggaac atcaatacca cccgcgaccg ccagataact gcgcatcccg
    25321 tgctgcgggc gtttaagcgt taaacgctgg cctgctttca tcggcaatcg ccagccggtc
    25381 cagacggcgt tatcatccaa ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca
    25441 tcagtttcga actcaactgt taactgaccg agcgtgatct ccagcgcggg ggcattggcg
    25501 tcattaccca ccagcaggtt agcaatgcgc agcgcaggca tatccagtgc gccacagtgg
    25561 ctgatacccg actggcgaaa accgtgacga ccgccatcct gcacagtggt gtacatgccc
    25621 gcacgaataa tcttcagcat attccctcct tctgcggtac aaatcgcacg ctgtctcccg
    25681 gacgtaataa gatgggttcg tcacgcgccg aatcaaacag gctgagtgag gtatgaccaa
    25741 tcaactgcca gccacccggc gttgccagcg gataaacacc agtctgcggc ccgccgatcc
    25801 cgacagaacc tgccggaacg agcaagcgcg gttcagcgcg ccgtggcgtg tgtagttgtt
    25861 ccggcaaact cccgagatac gggaagcccg gttgaaaacc taaaaaccag accacgtatt
    25921 ccacggagga gtgcaattca acaacctgtt tttcgctcaa cccgcaatac gccgcgacca
    25981 ccgccaaatc cggtccgcct gcaccaccgt aaaccaccgg aatttcaata aagcgagact
    26041 caggttccag ggcctcgctc tcctcccacc agcgttgcaa acgctctatg gcatccagcg
    26101 ccagcgactc aggattacgc aaaatcaccg tgatattgtt catgccggga atggcttcaa
    26161 ccacattcgg catatccacc agacgctgcg ccagtcgcca gatccgtttc tggctagcca
    26221 gcgtcaccgg cggttccagt tccagcacta ccgccgtttc acctatcaga taacaacgcg
    26281 ctcgttgcac tttcgatccc tctgattatt cgttatgcag gattaggaat atcaataaag
    26341 gtcacatcaa gatcggtatt ttcattcagc cactcgctca atgcgcgaat accaccgcgt
    26401 tcagtggcat ggtgacctgc agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg
    26461 gtctgttcag aaacttcgcc agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg
    26521 ataaaacttt gcccgccgcc cgtgcaccag gcgacgcgct ggaccacctc aggtccggta
    26581 tcgccacacc ataacggctt acgtcccaga cgcgcttcaa tccaggaggc tagttccatt
    26641 cccggcacag gcatggtcag ctctccccac ggcaccaacg gctcaatttc gcccatgacc
    26701 gtgatcccca gtaacgccgc cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc
    26761 ggcaaatgcc agccatacag gttgatatca ttcgccagca acgtttttaa acggttacgc
    26821 ttcattccgc gaatgaccgg agactctcct ttccagaagt agccatgatg cacaatgacc
    26881 gcatccgcgc ccagacgcac tgcctcatcg agcaaagcct ggctggcggt gacaccggta
    26941 acaatttttt gcaccgtctc tttgccttcc acctgcaaac cattcggcgc gtaatcacta
    27001 atcgccgcgc tgttcagttt ttcgttaatc agttgttcca gttcggtgtt tttcatcttc
    27061 gcgccttccg tattctgata gctacaccgt taacatagcg ttacctcgcc tgttcgtcga
    27121 tagccaatct tttgacatat taagaaacaa acccgacgcc aaaagacgca aattgtttat
    27181 atcatcacgc ttcagctaat gtatatttat gcaatcagac agcatattgc ataaataatc
    27241 agatctgtat caaagtttta actttaagga aacacaagca cgccgcgcaa ctattacgtg
    27301 acgttgccgt caattgtgga attattatga ataaacacgc atcacaaccg cgcgctattt
    27361 actatgtcgt tgcgctgcaa atctgggaat attttagctt ttacggcatg cgtgccctgc
    27421 tgattctcta tctcaccaat caactaaaat acaacgatac tcacgcctac gagttattta
    27481 gcgcctactg ttcgctggtg tatgtcacgc caatcctcgg tggctttttg gcggataaag
    27541 ttctcggcaa tcgcatggcg gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg
    27601 tgctgggtgc cagtgagatc catccgtcat tcctctatct gtccctggcg attatcgtct
    27661 gcggctatgg cctgtttaaa tctaacgtaa gctgtctgct cggcgagctg tatgagccaa
    27721 ccgatccgcg tcgtgatggc ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta
    27781 ttatcgcacc tatcgcctgt ggttacgccc aggaagagta cagctgggcg atgggctttg
    27841 gcctggcggc ggttggcatg atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt
    27901 tcactcatac ccgcggcgtt aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga
    27961 actggggatg gctgctggtt ctgctggtgg caacgcctgc actgattacc gtgctgttct
    28021 ggaaagagtg gtcggtatac gccttaattg tcgcgactat tattggcctg ggtgtactgg
    28081 caaaaattta tcgcaaagca gaaaaccaga aacagcggaa ggagctgggg ctgattgtga
    28141 cgctcacctt cttcagtatg ttgttctggg ccttcgcaca acagggcggt agctcgatta
    28201 gcctttatat cgaccgcttc gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga
    28261 tgttccagtc gattaatgcc ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg
    28321 ttaaagaaag cgtcgcaggt aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc
    28381 ttggcctgat gagcgccgga ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt
    28441 atggtcactc ttctctgcca ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac
    28501 tgtttatcga cccggttgcc atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg
    28561 gcgtattaac cggcatctac atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg
    28621 tgattgccga tcagacatcg caggcttcgt ttgatgcttc cggggcgatc aactactcca
    28681 tcaatgcata tattgaagta tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac
    28741 tggtactgat gatttggctg tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg
    28801 agtcataata ggccagcacc gatgcctgat gcgacgcttg acgcgtctta tcaggcctac
    28861 gtatttcctg caatttattg aatttgcaca aatttgtagg ctggattagg cgttcacgcc
    28921 gcatccggca tctgggctcg attgcctgac gcgtctgtta tttccccttc cgcgccgcct
    28981 catacgctgc caacgtttgt attctcgcct ctttgtgctc gactatcggt tgcggataat
    29041 ccagcttcac acctgctttc tgcgcccact tccacggctc atgcaccgct ttccctggta
    29101 catcgcgcag ctccggtaac catcgacgaa taaactcgcc ctcacggtca aatttctcgc
    29161 cctgagttat cggattgaaa atacgaaaat acggcgcagc atcagtaccc gtagaagccg
    29221 cccactgcca gccaccgtta ttagctgcca aatcaccatc aatcagctgc gacatgaaat
    29281 atcgctcgcc ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgtaatca
    29341 tccgtagccg gttatgcatc cagccagtgc tgttaagctg acgcatagcg gcatcaataa
    29401 tcgggtatcc cgttttgcct ttctgccagg cctgtaaatg tgcgggattg ctttgccact
    29461 gcacacgatc cgtccaggca ataaatggac gatgtttaca caacgagggg taatacgtca
    29521 tcagatgacg gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac
    29581 cgtccagcgc ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc
    29641 cgcccgttgc caagctggcg gacaaacggc tggtgccttc cactgccgga aaatctcgtt
    29701 gttgctcata ctctccagca ccgttctcgc aaaactggcg taattgcgca atcgccgctt
    29761 tttcttccac cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg
    29821 atggcgcggg ctttatcgat ccgctactac gaacttttgg tgcagcgacg cactccggca
    29881 tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact ttgtacatct
    29941 cgtgattgcc ggtcatcacc gcccccggcg gcaggatcac gctgtcatca aatccttcac
    30001 acaccacgtt acgcagcgct ctttcaacgt ccacatcccg cgctcgctca ttcacctcat
    30061 actgatagtt ataaaaaaga tgagtaacgc tgttttccgc gcacacctgt ttaactattt
    30121 cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct ttttccgcca
    30181 gcgcgatttg tagcccattc agttgagcat tgataagttc agcctgacgc ggcgacatgt
    30241 tatgcgtctc ccactggcgt ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat
    30301 tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcctgg cgaaaccagg
    30361 ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc
    30421 gaaataacgc tgccctgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg
    30481 catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgcagccag
    30541 ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac
    30601 gttggtgtga tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc gttggcggta
    30661 ctggttgtaa aaatcatcca gattttgcca ctgatgaata tcagccacaa acgggccaag
    30721 tttgcggtac tcgggctgag aatgggccag aaagacgagt ttatagcggc tgtgataatc
    30781 gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat gaagggcgaa
    30841 gatacggatt agggtgctgt catcaaatcg ttgtagattc attactgctc ctgaacggta
    30901 tacaggagta agtgtagacg ttggtaaatg atgtttgtga agttaatccg ctagcagggc
    30961 aagattacga aagcgcgctc cccgcaagca actaatcaag gtagttaatc atggctgaac
    31021 acgatattac gccagaagtc accatttcca aaacacagcg ccgctataaa gtcggctatg
    31081 tcagagcccg ccaccaggac cgcaaaaccg gtatagcccg gtattattca caacatccca
    31141 gcctgcacct taagggcgac tggctgaaag aggccggatt tgataccgga cggggtgtca
    31201 cggtgaagat ttcggagggg tgcctgacca tcatcgctga cagtgacgag gtgcaggaac
    31261 tgcgggagga actttatcag gttaaacagg tagttaaggg gatgcgggat gggatgttta
    31321 gtgtgctgaa cgaaagttaa atattaaaaa agctgggaag atttaaaacc ttcctagctt
    31381 tttattctgt cttattataa tggatactca tcatcttccg atgaatttat aaattgctcc
    31441 aagtttacat gaagagtact taacctagtt tttaactcat taagtgaagc gataatttca
    31501 tattttccaa cataatcata tgatg
//
LOCUS       DADKXM010000033        31208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.033, whole genome shotgun sequence.
ACCESSION   DADKXM010000033 DADKXM010000000
VERSION     DADKXM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.033"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            258..1118
                     /gene="tesB"
                     /locus_tag="JQR05_001511"
     CDS             258..1118
                     /gene="tesB"
                     /locus_tag="JQR05_001511"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY5323708.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1167..2453)
                     /gene="amtB"
                     /locus_tag="JQR05_001512"
     CDS             complement(1167..2453)
                     /gene="amtB"
                     /locus_tag="JQR05_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY5323709.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2483..2821)
                     /gene="glnK"
                     /locus_tag="JQR05_001513"
     CDS             complement(2483..2821)
                     /gene="glnK"
                     /locus_tag="JQR05_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY5323710.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3002..4783)
                     /locus_tag="JQR05_001514"
     CDS             complement(3002..4783)
                     /locus_tag="JQR05_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY5323711.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(4776..6548)
                     /locus_tag="JQR05_001515"
     CDS             complement(4776..6548)
                     /locus_tag="JQR05_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY5323712.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6578..7123)
                     /locus_tag="JQR05_001516"
     CDS             complement(6578..7123)
                     /locus_tag="JQR05_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY5323713.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7190..8008)
                     /gene="cof"
                     /locus_tag="JQR05_001517"
     CDS             complement(7190..8008)
                     /gene="cof"
                     /locus_tag="JQR05_001517"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY5323714.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATYCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8108..9808
                     /locus_tag="JQR05_001518"
     CDS             8108..9808
                     /locus_tag="JQR05_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY5323715.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAEQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            9873..10568
                     /gene="queC"
                     /locus_tag="JQR05_001519"
     CDS             9873..10568
                     /gene="queC"
                     /locus_tag="JQR05_001519"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY5323716.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(10620..11018)
                     /locus_tag="JQR05_001520"
     CDS             complement(10620..11018)
                     /locus_tag="JQR05_001520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY5323717.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11112..11483)
                     /locus_tag="JQR05_001521"
     CDS             complement(11112..11483)
                     /locus_tag="JQR05_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323718.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(11634..13505)
                     /gene="ppiD"
                     /locus_tag="JQR05_001522"
     CDS             complement(11634..13505)
                     /gene="ppiD"
                     /locus_tag="JQR05_001522"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY5323719.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(13697..13969)
                     /gene="hupB"
                     /locus_tag="JQR05_001523"
     CDS             complement(13697..13969)
                     /gene="hupB"
                     /locus_tag="JQR05_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY5323720.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14178..16532)
                     /gene="lon"
                     /locus_tag="JQR05_001524"
     CDS             complement(14178..16532)
                     /gene="lon"
                     /locus_tag="JQR05_001524"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY5323721.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDNSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(16720..17994)
                     /gene="clpX"
                     /locus_tag="JQR05_001525"
     CDS             complement(16720..17994)
                     /gene="clpX"
                     /locus_tag="JQR05_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY5323722.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18120..18743)
                     /gene="clpP"
                     /locus_tag="JQR05_001526"
     CDS             complement(18120..18743)
                     /gene="clpP"
                     /locus_tag="JQR05_001526"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY5323723.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(18990..20288)
                     /gene="tig"
                     /locus_tag="JQR05_001527"
     CDS             complement(18990..20288)
                     /gene="tig"
                     /locus_tag="JQR05_001527"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY5323724.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(20632..20949)
                     /gene="bolA"
                     /locus_tag="JQR05_001528"
     CDS             complement(20632..20949)
                     /gene="bolA"
                     /locus_tag="JQR05_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY5323725.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21254..21832
                     /locus_tag="JQR05_001529"
     CDS             21254..21832
                     /locus_tag="JQR05_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5323726.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            21876..23351
                     /gene="ampG"
                     /locus_tag="JQR05_001530"
     CDS             21876..23351
                     /gene="ampG"
                     /locus_tag="JQR05_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY5323727.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            23811..24758
                     /gene="cyoA"
                     /locus_tag="JQR05_001531"
                     /pseudo
     CDS             23811..24758
                     /gene="cyoA"
                     /locus_tag="JQR05_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            24780..26771
                     /gene="cyoB"
                     /locus_tag="JQR05_001532"
     CDS             24780..26771
                     /gene="cyoB"
                     /locus_tag="JQR05_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY5323728.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNCDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            26761..27375
                     /locus_tag="JQR05_001533"
     CDS             26761..27375
                     /locus_tag="JQR05_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY5323729.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMKIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            27375..27704
                     /locus_tag="JQR05_001534"
     CDS             27375..27704
                     /locus_tag="JQR05_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY5323730.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            27716..28606
                     /gene="cyoE"
                     /locus_tag="JQR05_001535"
     CDS             27716..28606
                     /gene="cyoE"
                     /locus_tag="JQR05_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY5323731.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            28791..29102
                     /locus_tag="JQR05_001536"
     CDS             28791..29102
                     /locus_tag="JQR05_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5323732.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSVAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            29086..29676
                     /locus_tag="JQR05_001537"
     CDS             29086..29676
                     /locus_tag="JQR05_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY5323733.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKFTRHYQLKGFILMLTLPPLTFMLVSALFSFQR
                     HQMLLVLYLCFWRFSIFSRLRLLSPPARASFALAGG"
     gene            complement(29683..30618)
                     /locus_tag="JQR05_001538"
     CDS             complement(29683..30618)
                     /locus_tag="JQR05_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5323734.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKKINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(30629..30862)
                     /locus_tag="JQR05_001539"
     CDS             complement(30629..30862)
                     /locus_tag="JQR05_001539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323735.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSHIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(30855..>31208)
                     /locus_tag="JQR05_001540"
     CDS             complement(30855..>31208)
                     /locus_tag="JQR05_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5323736.1"
                     /translation="QGAKKAALWLSWMYYNGLGETLDCDKVWDYYEKGAGKYSMKFNK
                     DEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPKICNVSIEINADKISSIT
                     NLQLTLELKGGGGSE"
BASE COUNT         7318 a         8381 c         7510 g         7999 t
ORIGIN      
        1 tcgcaaccgc taaaccactg gccatgtgca cgagtttcat tcatttctcc ttattatcaa
       61 tgcaccagcg ggctaacttt cctcgccgga agagtggtta acaaaatagt aacgtcaaca
      121 agtgtggcac acatcacgca tttctgcctg taattagccc gtaattcaga ctattgcacc
      181 catcggacca gtggcaaatt tgcgttatac tcaactcact ttggcttgct gcggcagctt
      241 tgttactgga gagttatatg agtcaggcgc taaaaaattt actgacattg ttaaatctgg
      301 aaaaaattga ggaaggactc tttcgcggcc agagtgaaga tttaggttta cgccaggtgt
      361 ttggcggcca ggtcgtgggt caggccttgt atgctgcaaa agagaccgtc ccggaagagc
      421 ggctggtaca ttcgtttcac agctactttc ttcgcccagg cgatagtaag aaaccgatta
      481 tttatgatgt cgaaacgctg cgtgacggta acagcttcag cgcccgccgg gttgctgcta
      541 ttcaaaacgg caaaccgatt ttttatatga cggcctcttt ccaggcacca gaagcgggtt
      601 tcgaacatca aaaaacaatg ccgtccgcgc cagcgcctga tggcctgcct tcggaaacgc
      661 aaatcgccca atcgctggcg cacctgctgc cgccagtgct taaagataaa ttcatctgcg
      721 atcgtccgct ggaagtccgt ccggtggagt ttcataaccc actgaaaggt cacgtcgcag
      781 aaccacatcg tcaggtgtgg atccgcgcaa atggtagcgt gccggatgac ctgcgcgttc
      841 atcagtatct gctcggttac gcttctgatc ttaacttcct gccggtagct ctacagccgc
      901 actgcatcgg ttttctcgaa ccggggattc agattgccac cattgaccat tccatgtggt
      961 tccatcgccc gtttaatttg aatgaatggc tgctgtatag cgtggagagc acctcggcgt
     1021 ccagcgcacg cggctttgtg cgcggtgagt tttataccca agacggcgtg ctggttgcct
     1081 cgaccgttca ggaaggggta atgcgtaatc acaattaaaa aaatagccgg aggtgaaaac
     1141 cgtccggcta ttttttgcag tgcttgttac gcgttatagg cattctcgcc gtggctgttg
     1201 acgtccagcc cttctcgctc ttgttcttcc ggcacacgta ggccaaccgt cagatccgcc
     1261 agtttgtagc caataaatgc caccacaccg gaccagacga tcgtaatggc gatgctttcc
     1321 aactgtacca gcaactgatg gcccatcgtc acaccttcag cgaagcccac gccgcccaga
     1381 gaactggcgg caaaaatccc ggtcatgata cagccgacaa tgccacaaac gccgtgcaca
     1441 ccgaagacat cgcagggatc atccacccgc agcaagcgtt tgagcatggt aacgccccac
     1501 aaacccgcca gaccagctac cacgccgata atcaacgcgc cgccaacccc aatgtagccg
     1561 cacgctggcg tcacgccgac cagaccggca atcgcgccag aacacgctcc cagcagagaa
     1621 ggcttaccac gcagcgccca ttcaccgaag atccagccaa gaattgccgc cgccgttgcg
     1681 accacagtat tcacaaatgc cagtgccgcg atttcattcg ccgtgcccgc tgacccggcg
     1741 ttaaagccaa accaaccgat atagagaatg gcagtcccgg tgaagaccat cggcaggttg
     1801 tgcggtttaa acgcctcttt accgaagccc acgcgtttgc ctatcagata cgcgcccacc
     1861 agaccggcga ttgctgcgtt aatgtgcacc accgtgccac ccgcgaaatc cagcgcaccg
     1921 tgagaagcca gcaaaccacc gccccacacc atatgcgcaa tcggaatgta agagagcgtc
     1981 agccatacca ccacgaaaat caacacagct gagaagcgga ttcgttccgc cagcgcccca
     2041 actatcaagc ctacggtaat gcaggcaaac gatccctgaa acgctacgtg gatatactga
     2101 taaatgctgc ccatcaccgc cgtcagttcg atgtttttta gcatcaacca gttaatgttg
     2161 ccgaagaagt tgttgccctc accaaacgcc agcgaataac cgtaaaccac ccagagaata
     2221 cagaccagtg caaacgtcac cgtcacctgc gtcagcatcg acagcacgtt tttgccgcga
     2281 atcaacccac cgtaaaacag ggcaatcccc ggaatagtca taaacagcac cagcgcagta
     2341 caaatcatca taaacgcatt gtcggcttta tcggccaccg caggtgcagc cattaccagt
     2401 cccggcagca tcgccagtga agcaagccca gtttttatcg tcgctatctt catttttcgt
     2461 tcctgttgct gtgtgccaga gattacagcg ccgcttcgtc ggcttcgccg gtacgaatac
     2521 gaatgacgcg ttgcaattca gcgacgaaga ttttgccgtc gccaattttt ccggtgtaag
     2581 ccgccttact gacgatatcg atcacttcat cgagttggtc atcagcaatc gccacatcaa
     2641 tttttacttt tggcaggaag ttgacgctgt attccgcccc ccggtacagc tcggcatgcc
     2701 ctttctgacg cccgaaacct ttcacttcgg tgacggtcag gccctgaata ccaatggaag
     2761 ataacgcttc acgaacgtct tccagcttga atggtttgat tatcacggtc accagcttca
     2821 tagatcccct ccggtcagaa ttcggtaatg gttctgctac acgaagaagg tattgcaagc
     2881 ggtgtgccag aaatgaaaaa cgagaaagag agcaggaagt taagaagatt gaaaatgaaa
     2941 acgcactatg acagtgcaca gtgcgttaca tttttgcacc aacgatgaaa tattgcgcta
     3001 ttcaggcgct caatgattcc tcttcacgca cgctggctgc cagctcttcg cccgcaagtt
     3061 gcagttgata catctgccag tagcgtccct gggccgccag cagttgctgg tgagtgccct
     3121 gctccacggc ttgcccacga tgaagcacca gaatggtgtc ggcatcaaca atagttgata
     3181 agcggtgagc aatcaccacc agcgtggtat gttcacgcac cgccgccaga gcatgttgaa
     3241 tcgcctgttc agtaccggag tcaatgctgg cggttgcctc atcaaggatc aggatttgcg
     3301 gcgtctccac cagcacgcgc gccagtgcca gcagttgctt ttgcccgact gagagattat
     3361 ttccctgctc gcccagcggc gtataaatac catcactcat gctgcgcgcc agctccgcca
     3421 gttgcacggt ttccagcgcc tgccagacgc gttcttcgga gatatcccgc cccagcgtca
     3481 cgttggcgag gaaggtatcc gccagcacca ccggatcttg ctgcaccatt gccacgccct
     3541 ggcgcagcgc gctatgactt agcgaactta atggacgacc atcaaggtga atctcacctt
     3601 ccgttagcgg gtaatagccc atcaataaac tggcgagggt acttttacca ctgccggtat
     3661 gcccgaccag cgccacaaag ttacgcgaag gcacagagag attaatgttc tttagcacca
     3721 gattgtcatc gcgataagca aatgacacgt tatcgacttc gatggtgcca ctctgtaacg
     3781 ggtaatcatc attgccatat tgctggcgcg gtccgtccat cagttcaaac acgcgctcac
     3841 cagcaacaac agcctgttgc agcatcgcct gttgcgtggt cagttcgatt aagggttcgt
     3901 taagtcgccc aagatagctg ataaacgcat acaacacgcc cacttcaatg gtgccgctgg
     3961 cggagaagcc aaacagcatc aataagccac aaagaatgag cgatgaaaac agactcagca
     4021 gcggacgcag cagaaaaccg tcgaggcgca gggtttgcat cctcgccata tagtgtgaac
     4081 gactggcctc ccccatacgt tcgccaaatc gcgcctgctg acgaaactgc tggataacgc
     4141 tcatgccatt gatgatttcg ttaaagccgt cgttgatatc cgccagatag gcgcgcacac
     4201 ggcggacaat cggcgtgctg taacgctggt atatcaccat taccaccagc accaccggga
     4261 aaatcattat cgccaccagc gccattcgcc agtcgaggct gaacatcgcc accagcatcg
     4321 cgcccaccag tgcggcactg cgcaggacag ttgccactac ggtaacgtag agatcgcgga
     4381 tcacttcagt gtcattagtg acgcgggaaa tcacctgccc gacgggttgg gtatcaaact
     4441 cgcttaatgg ctggcgtaac gcagcatcca tcacgtcggt acgcaactgt tgcactacgc
     4501 ctactgccgc ccgattaaac agcagcgact gcgcgtaatg tagcccggcg gcaaacagtt
     4561 gtagcccaac atacgccgca gccagccctg caaccacttt caacggcagg ttatttttcg
     4621 ctaccatatt gtcgataaaa tagctgataa gcagcggccc actgacttct gccgccgccg
     4681 caacccacat catcaggacc gcaatcccca gcggtttacg ccacggcgaa ccatacgcta
     4741 acaggcgctt gagagtcggc cacagttggc taaaactacg catcgacggc ctcctcgcga
     4801 atttccggaa cgtcgtcgag cgccgcctcc agttgttgat agcgatacat atcgcgatac
     4861 cagccgcttt gttgcgccag ctcatcatga ttgccacgct gggcgatatg tccgtgctgc
     4921 atcacaataa tttcactggc ttccgtcagt gccgaaaggc gatgggcact gatgattacc
     4981 gttcttccct gtccccactg acgcaggtta tgcaggatct ggtgctctgt gcgtccgtcc
     5041 accgccgaaa gcgcatcatc aaggatgagg atttccgcgt tgactaataa cgctcgagca
     5101 atggagatac gctgtttttg cccaccggaa agcatcacgc cgcgctcgcc cacttctgta
     5161 tcgtaacctt gcggtagacg caaaatatcg tcatgtacgc tggctaaccg cgcgacatgc
     5221 tcaatctctt gctgggtggc attcgggcaa cccagcgcga tgttattcgc cacggtgtca
     5281 gaaaaaagga atggcgtctg gctgaccacc gccaggcggc tacgccagct atcgagttgt
     5341 aacttcgtca gaggaatatc atgaaagcga atatccccct cgctgacgtc gaaatgacgc
     5401 tgaatgagcg acaacagggt acttttgccg gaaccagtcg gcccgcaaat acccagcatc
     5461 tgaccgggtt tcagggcgaa attgacgttt tccagcgcag gatggtcagt ctgcggatac
     5521 gtgaactggt gaatatttac atccagttcg ccacgccctt ccggcacggg ttcgctacca
     5581 tcgttcacca ccggcgcttc cgccagcatc gcgcgaatac ggctgtatgc agcactacca
     5641 cgttccacaa tgttaaacat ccatgccagc gccagcattg gccaaatcat cagacctaaa
     5701 tacatcataa aactggtgag ctggcccagc gttaaactgc cctgcaccac catccaacta
     5761 ccgccgccaa tcgccagcaa gttcgccata ccaatcgcga tatagatggt cgggtcgaaa
     5821 cgagcatcaa tacgcgccac ccgcatgttt tttttgccgg tatcttcggc atccgcggca
     5881 aataacgccg actggcgatc ttccagacca aaggctttga tcatgcggat actggtgagg
     5941 ctttcctggg tgcggtcatt aagactggaa aacgccgcct gtgccagctt aaagcgttca
     6001 tgcaaggcat cgccgttgcg cttaatcatg atcgccatca ctggcatcgg taacagggca
     6061 aataaggtca actgccagct aatttgcgta gacatcataa tcaacacagc gcagcccatt
     6121 accagtgaat ccaccagcgt cagcacccct tctccggcgg caaacacgac gcgatcgacg
     6181 tcattggtcg cacgagccat gagatcaccc gtgcgatgac gcaggtaaaa ctccgggtgc
     6241 tgccggctta gctgacggta ataatcttca cgcagttcaa cagccagttg gtaagacgcg
     6301 ccaaacagca atacgcgcca gacataacgc aggagataaa ccacaacggc aatcagcacc
     6361 atggtggcga tccacatcag gatcagcccg gtagtaaagt gttgttctgt cacgccatcg
     6421 acaacaatac caaccacttt tggcggaacc agttgcagca tcgcgataat gacaagcaag
     6481 gcgacagccc caagatagcg acgccattcc cgacggaaat accagcttaa ttgagcaaat
     6541 aatcgcacgc gttgtgtcct gacctgattc tggatattta ttcgatgggt aaagaagtgg
     6601 tgtatttaat ctgttccatc gcgaagctgg aagtgacgtc cgacagcccc ggcacgctgt
     6661 ttaccagacg cttataaaac tcgtcgtagc gtttcatgtc ggcaacctgg acgcgcatca
     6721 gataatcgta ttcaccagcc atgcgccaga accccagcac ttctggcatt tcggtaacca
     6781 ccgtgacaaa gcggcaatac cattcgctgc tgtgatgttg cgtttttatc agcacaaagg
     6841 cggtcaggcc gaggcctatt ttttccggat ccagcagggc gactttgccg ataaggatac
     6901 cgtcgtcctc cagccgtttc aggcgcttcc agcagggggt ggttgtcaga ttaacggctt
     6961 cagccagtgc ctgcaaagag agggtgcaat cctgctgcag taaggccagc agcttacggt
     7021 caattttatc taacatagcc cttccacgga gaattttttt ctcttttaat tctattttaa
     7081 aggtgaaatc gcgacaattt attctgtgcg gtttaaacac cacggcacaa aatgacaaat
     7141 tcaacgcaaa aaaaaaccgg gcaattgccc ggtgctggaa gggatctcgt tattcggggg
     7201 aataaggtag atgtggatag tccagccagt gcgtcaaata gtgagagaca gcctgatttc
     7261 ggcaatgtcc aatcaccggt aaatgcggga gctccgcgcg cagttgcggc atcgcattgc
     7321 ccataataaa cccgctaccg acgctgccta acatttcgcg atcgttcatc gcatcaccaa
     7381 aggccatgca atcgcgcaac gataaaccta aatgttgggt cagcaccgtc aatgcagcgc
     7441 ctttattgca gcccaccggc agcacttcga ggcaatacgt ggcggaaaaa cacaaatgtg
     7501 cacgctcgcc taatgcttcg tatagctgga tctgcaagcg tgtaagatcg tcgtgatcgc
     7561 cacagaagca gatcttggtg acgctgccga gtggcatttt tttgacatcg attatctgat
     7621 aacgaaaacc gctatagaca aatgcctgca acaacgcagg gatctctttc ccggtaaacc
     7681 aaccgtcgtc attgaagata tgcatgctgg ctcgggtatc ccattgctga tacagcacca
     7741 gctccgcgac atccgcaggt aaatcatcac gatgtaaaag ttcaccttcc agagaatgca
     7801 cgcgcgttcc gttgccggta atcaaatatg catccagcga tagcgccccg agaatatgct
     7861 gcatctccag cgcatgacgc cccgtggcaa aagtgagggt aatgtcgcgt tcacgcagtc
     7921 gcgccaaagt agagagggtt ttctcaccta aatgatggtc aggcatcaat aaagtgccat
     7981 ccatatcaaa tgctgccaga cgagccattt ctttctccac tctgtgacac ggttaatggt
     8041 gattgagtat cacctgatat atacggaagt aatagtgaat agttaaataa tattgttccg
     8101 ggtttatatg cgattgctca accgtcttaa ccagtatcaa cgtctgtggc aaccttcagc
     8161 cggaaagccg caaaccgtca ccgtcagcga actggccgaa cgctgttttt gcagcgaacg
     8221 ccatgttcgt acgctgttgc gtcaggcaca ggaggcggga tggctggagt ggcaggcgca
     8281 gtcaggacgc ggaaagcgcg gacaattacg ctttctggtc acgccggaat cgctacgcaa
     8341 tgcgatgatg gaacaggcac tggaaaccgg aaagcagcaa gatgtgctgg agctggcgca
     8401 actggcccca agtgagctgc gcactctgtt acagccgttt atgggcggac aatggcaaaa
     8461 cgatacaccc acgttgcgta ttccctacta tcgcccgctc gaaccgctac aaccaggctt
     8521 tttgcccggc cgtgccgagc agcatctcgc cgagcagata ttttccggcc tgacccgctt
     8581 cgataataat actcagcgcc cgattggcga tttagcgcat cactgggaaa cctctactga
     8641 cgggttacgc tgggactttt atcttcgttc aaccctacac tggcataacg gcgatgcagt
     8701 aaaagcctca cacttacacc agcgattatt gatgctgtta caactgccag cactggatca
     8761 attatttatt agcgtgaagc gtattgaagt cacccatccg cagtgtctga ccttcttttt
     8821 acatcgcccc gattactggc ttgcgcaccg gctggcgagc tattgcagcc atctggcgca
     8881 tccgcaattc ccactgatcg gcactggtcc ttttcgctta acacaattca cagcggagct
     8941 ggtgcgcctg gagagccatg attattacca tttgcgtcat ccgctgctta aagcggttga
     9001 gtactggata actccgccgc ttttcgaaaa agatttggga accagttgtc ggcatcccgt
     9061 gcaaatcacc atcggcaaac cggaggagct gcaacgggtc agccaggtca gtagtggcat
     9121 cagtttaggt ttttgctatt taacgttgcg caaaagtccc cgactctccc tctggcaggc
     9181 gcgaaaagtg atctccatta ttcatcaatc cggtttatta caaacgttag aagtcggaga
     9241 aaacctgatc accgccagtc atgcattact gccaggctgg actattccgc actggcaagt
     9301 accggatgaa gtcaaactac cgaaaacctt gacgctggtt tatcacctac cgatagaact
     9361 tcataccatg gcagaacgcc tacaggcgac actggcagcg gaaggctgtg aactcacaat
     9421 tatttttcat aacgcaaaaa actgggacga cacgacccta ctggcacacg cagacctcat
     9481 gatgggcgac agattaattg gcgaagcacc ggaatatact ctggagcaat ggctacgttg
     9541 cgatccacta tggccacatg ttttcgacgc tccagcatat gcacatctgc aatcgacgct
     9601 ggacgcggtg caagtaatgc ctgatgagga aaaccgattt aatgccctga aagcggtttt
     9661 tagccagtta atggcagacg cgacgctgac gccgctgttc aactatcact atcgcattag
     9721 tgcccctccc ggcgtgaacg gtgtgcgact gacaccgcgc ggctggtttg aatttaccga
     9781 agcctggctt cccgcgccgt cgcaatgaat agctggtccg ggttaatcgc aggcgttacc
     9841 ataacgcttt tattgttcaa gcaggattat ctatgaaacg tgctgtcgtt gtgttcagtg
     9901 gaggccagga ttccaccacc tgtctggtgc aggcattaca acaatatgat gaagtccatt
     9961 gcgtgacgtt cgattacggt cagcgacatc gcgcagaaat cgacgtggca cgcgaactgg
    10021 cgctgaaact gggagcacgc gcgcataagg ttcttgatgt aacgctgctc aacgagctgg
    10081 cggtcagcag cctgacacgt gacagcattc cggtgcctga ttatgaacct gaagccgatg
    10141 gcatcccgaa tacgtttgtc ccagggcgta atattttgtt cctgacgctg gcggcaatat
    10201 atgcgtatca ggtaaaagca gaagccgtaa ttaccggcgt ctgtgaaacg gatttctccg
    10261 gctacccgga ttgccgcgat gagtttgtga aagcactaaa ccatgccgtc agtttgggca
    10321 tggcgaaaga tattcgtttt gaaacgccgc tgatgtggat tgataaagcg gaaacctggg
    10381 cgctggcaga ttattacggc aaactggatt tagtccgtaa cgaaacgttg acctgctata
    10441 acggcattaa aggcgacggt tgcggtcatt gtgcggcatg taatttacgc gccaacggtt
    10501 tgaatcatta tctggccgat aaaccgacgg tgatggcagc gatgaagcag aaaaccgggt
    10561 tgaggtaatt attccgggtg tcgccggatg cggcttgagc atccggcacc acaaaacgtt
    10621 tacttaacca tctgctccag cttttcgcgc aattcccctt ccagagctaa tgctttctgc
    10681 gttttaagat caatacaaac aaacgtaata agcgcatccg ctaccacctg cccttccggc
    10741 tccagtgtaa tgacctggct taagatgccg cttttaccgt ttaattgctg caactgactg
    10801 gtaatagtta acaggtcgct taataccgct gggcgacgat agttaatatt gatattgacc
    10861 acgacgaagg cgatgttatg ggccgtcatc cactgaaaac tgtcgctatt ttccaaccca
    10921 tcccagcggg cttcttcgag aaattcaagg tagcgggcgt tgttgacgtg ctggtaaacg
    10981 tcgagatgat atccacgaac tttgatttgt gtttgcatag cgcaataacc ttacgttgtt
    11041 gttataagta cagaggtcat aactggtatg acctcttcag tctggcaaaa aattgccact
    11101 atgcaaatta attacagggt taataccgcc agattacgtt ccaccagcga attgcccatc
    11161 cccggcacct gctttagatc ctccaccgtt ttaaacggac cgtactcttc gcgataactg
    11221 acaatcgcct gcgctttctt caggccaacg ccattcatcg cgcgggctag ctcttccgcg
    11281 ctggcattat taatgctgac ccgggtgcct tcttcgtcac tggctttcgc cggtactgct
    11341 gctttacttt gtgctgcagg agcttccgct ttggtttcta ccgccgtcgg tttcgccaca
    11401 gaagctgccg ccagcgcgct atgagacatt ccggcacagg ccagggacag ggtaatgagc
    11461 agtgctttaa ttccgtgttt catactgttt tctccttgtt tgttaacagt ggaatcacct
    11521 taacgggagt gaaaacagta aacaaatggc aatgttcaga aatggaaaag gccgcgaaag
    11581 cgaccttttc ttgttacagc tgcaacgcat ttttttgcga ggcggcttca ggattattgc
    11641 tgttccagcg catcgccaat tttgattttc gcctctttac gcaggttact catcagagct
    11701 tcaaagacga tttgtgcgtt gttctgggtg ataccctgca ccatcgcttt tttctgatct
    11761 tccggcattg aaccttgttt cacttcatcc agcgccagca gaaccacatt accttgcata
    11821 tcggtcgcca taccgtagct cggtttgtct ttcgctggca gtggcagtgc aaacgccgcc
    11881 tggctaatcg ggtcacgacc ggaacggctt aaggttttcg gctcgccaaa tttcagaccg
    11941 gcagcctgca tagcttccgc acctttgccg gctttcaaat caaccagcag tttctcagca
    12001 tccactttcg cctgttgttc agctttgttg tgctgaacca gtgccttaac ttgttcctga
    12061 acatctgcca acggtttcac cgcttccggt ttgtgctcgc tgatgcgcag cacgaatgcg
    12121 cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg gcgcgccgtt ttcacctacc
    12181 agaccgccgt taaagatagc gtcggcaacc ggcttgaagt tcaactcttc cggcaggtta
    12241 tctttgctga accaacccgt ctgagtggct ttaacgccgg cagcttgctc tgcaccggcc
    12301 agagactcgg tgtcgttgct tgccgcatcg ctcactttct gctgcagcgc atagtacgca
    12361 tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt cacgtacttc gtctaacgat
    12421 ttcactttcg ctggctgaat gtcgtccaga cgtacaatca ggaaaccaac cgaagatttg
    12481 atgacaccag acagttggcc tttttctttc agaccagcat ttttcagttc gtcggggata
    12541 gtggcatctt ctaaccaacc catatcgccg ccgttacggg cagagataat atcggcagat
    12601 ttttctttgg ctaatgcagc aaaatcaccg cctttattca gctcatcaag taccgctttc
    12661 gcttcatctt cagttttggt ctggatgatg ctgtagcggg tacgctgcgg ctgggtgaat
    12721 tgatcctgat gctgatcgta gtagctctgg atatccgcat cgctaaccgg ttgctgcatc
    12781 gttgcggcaa ccagcttgat gtaactcacg cggaattgtt ccggcgtcat gaaattgttt
    12841 ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca caggctgctt cgccgccagc
    12901 gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt gcgcgaccag tgccgccagc
    12961 tcgtcggttt cacctttcag cataaaatcg gtaccggcaa cgccgttaat cagctgttgg
    13021 gtagtgagct ggttacgcag cgcctgggcg tactgatcgg cggtcatccc catctggttg
    13081 aggataccgt tatagcggct gttatcaaat ttgccgtcaa cctggaaggc tggggtcgcg
    13141 aaaatcgcct gtttaacctg ctcatcactg atacccagtt tcagctcacg agcgtactga
    13201 tccaacagcg cctcgtcgat cagacgattc agcacctgtt gacgcagggt tttcatatag
    13261 ccttcgttcg ctgccagctc ggagtattga tcgcccagct gttgctgcat gcgattacgc
    13321 tcgctgttaa aggcgttctc gaactgccca cggctgattt cctggtcatt cacttttgcg
    13381 gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc cggtcaatat gaacgacacg
    13441 ataatgatac cgaaaataat cttgagcacg agactgtttg cagccgtgcg taagctgtcc
    13501 atcatggtgt aacaacactc cgctgtagat gactgtgtac ctcacgcaac gtatcgatgc
    13561 gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc agcccacaag acacagaaag
    13621 tcaccgaata caaataaaaa aggcacatca gtagatgcgc ccttgaactt cgtcacatcc
    13681 ccactgggga caacgcttag tttaccgcgt ctttcagtgc tttacctgca cggaagctcg
    13741 gtactttagc ggcagcgatg gtgatctctt taccggtctg cgggttgcgg ccagtacggg
    13801 cagcacgctc tttaacggca aaagtaccaa aacctaccag tgctacatca tccccttctt
    13861 tcagagattc agttacggaa gcaataatag catctaacgc acggccagcc gcagctttag
    13921 agatatcagc ccctgcagca atcttgtcga tcaattgaga tttattcact cttctcttcc
    13981 tctttataat ttatatcgca cttgaatcct tcaaggtacg aacgcgcagc agttatatca
    14041 ggccagccat ccccttacaa caccccttaa taagggcaag cccgatccgc tatctaactt
    14101 agcgagacaa aaaaaggctg gcaagcccga attagcctgc cagccctgtt tttattagtg
    14161 cattttgcgc gaggtcacta ttttgcagtc acaacctgca tgccagacgg ttcattttgc
    14221 agcgccagag tcagaacttc ctcaatgcgc ttcacaggat gaatgtccag atcggcaatt
    14281 acgttgtcag gaatctcttc cagatcgcgt ttattttcga acggaattag cactgtttta
    14341 atcccgccgc gatgcgctgc caggagtttt tctttcaaac caccgatcgg cagtacctga
    14401 ccacgcagag tgatctcacc ggtcattgcc acatcggcac gaaccgggtt accggtcagg
    14461 caagaaacca gcgcggtgca catagcaata ccggcactcg gaccatcttt cggcgtcgca
    14521 ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa aatcagggtt gatccccagt
    14581 ttttccgcac gcgcacgaac caccgttaac gccgcctgaa tagactcctg catcacttcg
    14641 ccgagcgaac cggtataggt cagtttgcct ttacccggaa cacatgcggt ttcaatggtc
    14701 agcaagtcac cgcccacttc cgtccacgcc agaccggtta cctgaccgac acggttttcg
    14761 ttatcagcgc gaccatagtc gaaacgctga acaccgaggt agtcatgcag gttatcgccg
    14821 ttaatttcga tatgttttaa tgaattatcg agcagtaact gcttaaccgc tttgcggcac
    14881 agtttggaga tttcacgctc cagaccacgc acgcccgcct cacgggtgta gtaacgaata
    14941 atgccgataa tggcgctatc gtcgacggtc agctcacctt ttttcagtgc attacgttca
    15001 atctgcttcg gcagcaggtg acgtttggcg atgttcagtt tttcatcttc ggtataaccg
    15061 gagaggcgaa tcacttccat acgatccagc agcggtgccg gaatgttcat ggagttcgac
    15121 gtcgcgacaa acatcacgtc gctgagatcg taatccactt ccaggtagtg gtcgctgaac
    15181 gctacgttct gctctggatc cagcacttca agcagtgcag aggccggatc gcctcgcatg
    15241 tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg ggtttttcac gcccactttc
    15301 gccattttct ggatcagttt acccggcata gaaccgatgt aagtacggcg gtgaccacgg
    15361 atttccgctt catcacgcac gccgcccagc gccatacgga catatttacg cccggtggct
    15421 ttggcaatgg actgaccaag agaggtttta cctacccccg gcggccctac caggcagagg
    15481 atcggtccct tgattttgtt gacacggctt tgaaccgcaa ggtactcaag gatgcgatct
    15541 ttcacgcgct ccagaccata atggtcggta tcaaggattt cctgcgcctg acgcaggtct
    15601 tttttgacct tgctgcgcgc attccacggt acctgtacca tccagtcgat ataaccacgc
    15661 actacggtcg cttctgccga catcggagac atcattttca gcttctgcaa ctctgcttcc
    15721 gctttctctt ttgcctcttt cggcatcttc gccgcgtcga ttttgcgctt cagggcttcg
    15781 ttttcgtccg gcgcgtcgtc catttcaccg agttctttct gaatagcttt catttgctcg
    15841 ttcagatagt actcacgctg ggatttctcc atctgctttt taacgcggtt gcgaatgcgt
    15901 ttctcaacct gcagcagatc gatttccgat tccatcattg ccatcagata ttccagacgt
    15961 tcgttaacgt cggacatctc cagaacggac tgtttgtcag ccagtttcag cggcatatgt
    16021 gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc tattcagcga cgtcagcact
    16081 tctggtggga tttttttgtt cagcttgatg tagccttcga actggctgat tgcagtacgc
    16141 accagcactt cctgttcccg ctcatcaatg gtcggcgact ccagatactc cgccttcgca
    16201 gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg cgcgctgtaa cccctcgacc
    16261 agcactttga cggtgccgtc aggcagtttc agcatctgca atatagaggc cacggtcccg
    16321 acggtgaaaa gatcgtttac acccggctca tccgttgaag cttctttctg cgcgaccagc
    16381 ataatttttt tatcatggtc catcgccgct tccagacaac ggatagattt ttcccgcccg
    16441 acaaataagg ggatgaccat gtgcggataa accaccacat cgcgcagcgg caatacgggg
    16501 atttcaatgc gttcagaacg ctcaggattc atagagctct ctcttagttt aatttccgcc
    16561 aggtaatcag atgacacgac tgtgcttcac gccatctatt aacatgtacg tcagtatatg
    16621 gggatgtttc ccccacattc aacgccgaga atagaggaaa aattaaaggg gagataaaat
    16681 cccccctttt tggttaacta attgtatggg aatggttaat tattcaccag atgcctgttg
    16741 cgcttccggc ttaccataaa tcagcaacgg tttgctttga ccatcaatta ccgactcgtc
    16801 gataaccact ttttcgacgt cttccatgga cggcagatcg tacatggtat cgagcagtgc
    16861 ggcttctacg atggaacgca ggccacgggc accggtttta cgcgccatcg ctttcttagc
    16921 gatagcatcc agcgcctcgt cacggaattc cagatccacg ccttccagat taaacagcgc
    16981 ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg atctgaatca gagcttcttc
    17041 gctcagttca ttcaacgttg ctacaaccgg cagacgacca atgaactcag ggataagacc
    17101 aaacttgatc agatcttccg gttcaacctg cgccagcagc tcgccttcgc ttgctttgtc
    17161 ggacttcgct tttaccgtcg cgccaaaacc aatgccggag ccggtttcta cacggtggga
    17221 aatcacttta tccagaccgg caaacgcacc gccacaaata aacaggatct tagaggtatc
    17281 aacctgcaag aattcctgct gcggatgttt acgcccacct tgcggtggaa cagcagctac
    17341 cgtaccttcg atcagtttca acagtgcctg ctgtactcct tcaccggaaa cgtctcgggt
    17401 aatggacggg ttgtctgact tacgagaaat cttgtcgatt tcatcgatgt agacaatccc
    17461 gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac agcttctgaa tgatgttttc
    17521 aacgtcttcg cccacataac cggcttcggt cagcgtggtt gcgtcggcca tggtgaacgg
    17581 gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt ttaccggaac cggtcggacc
    17641 gatcagcaga atgttacttt tgcccaactc gacaccattg ctggtatcgc cgttgcgcag
    17701 acgtttgtaa tggttgtata ccgcgaccgc cagcactttt ttcgcctgtt cctggccgat
    17761 aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc ggtagcgcac tgcgttcacg
    17821 atgcggtgca acttctttaa tctcttcgcg aatgatgtcg ttacataaat caacacattc
    17881 gtcgcagata tacacggatg gaccggcaat cagcttgcgc acttcatgct ggcttttgcc
    17941 gcaaaaagag caatacagca atttgcctga gccatctttg cgtttatctg tcatgggtca
    18001 aaacctcttc tttgttcttt gtgccgcaca cgacgacgca aatgccattc tcaggcgcaa
    18061 gccgcttaca gcgttgtgcc gccctagata agtatagcgg cacagttgcg cctctggcat
    18121 caattacgat gggtcagaat cgaatcgacc agaccgtatt ccaccgcttc aggggcggaa
    18181 aggaagcgat cgcgctcggt atcacgttca atctgttcta atgattgacc cgtatgaagc
    18241 gccataagtt cattcatgcg ccctttaact ttcagaattt cacgggcatg aatttcgata
    18301 tcggtcgcct ggccctggta gccgcccaac ggttggtgaa tcatcacgcg cgaattcggc
    18361 aggcaaaaac gtttaccttt tgcccctgcg gtcaacaaga aagcgcccat cgaggccgcc
    18421 tggcccatac agatggtgct gacatcaggc ttgataaact gcatggtgtc atagatagac
    18481 atcccggcag tgatcacccc gcctggggag ttaatgtaca gatagatatc tttttctggg
    18541 ttttccgctt ccaggaacag catctgcgcc acaatcaggt tagccatgtg gtcttcaacc
    18601 tggccagtca gaaaaatgac gcgttcctta agtagacgag aatagatatc aaaagagcgc
    18661 tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg ccatatgggg tgcaaagtta
    18721 tctcgttcgc cgctgtatga catttccgtc tcctggataa aattgaaaaa aacctgctgt
    18781 accgattgta accttatgga cggattatca gctagtccct gtgttatggg tacggcatca
    18841 ccatatttca agcataacaa tcttttgttg ttacgctaac accgaaagag gcttttcgca
    18901 cggctccatg caaaattcat gacaaaaaaa agcccaccac ctggcggtga cgggcctttg
    18961 tgcgaattta gcgcgttatg ctgcgtaaat tacgcctgct ggttcatcag ctcgttgaaa
    19021 gtggtttctt tttcagtcac tttcgctttc gccagtacag cttcaacagc ctgttcttcc
    19081 agagcaacat tgcgcatgtt gtccatcagt tctttgtttt tgctgtagaa ctcgataact
    19141 tctttcggat cttcgtacgc agaagccatc tcttcgatca ggcctttcac gcgctcttcg
    19201 tcagctttca gctcgttggt gcggataact tcgcccagca gcaggccaac aactacgcgg
    19261 cgtttagcct gttcttcgaa cagttcgcgc ggcagttcca gagcttgttt ttcgttgcca
    19321 ccgaaacgct gtgcagcctg gcgacgcaga acgtcgattt cgctgtcgat cagcgcagcc
    19381 ggtacgtcga tgtcgttagc ttttaccaga ccttcgatcg cctgagactt aacgcggtta
    19441 cggatggcgc tcttcagctc gcgctccata tttttacgca cttcagcgcg cagaccttct
    19501 acggaaccat cttcaacgcc gaaacgtttg atgaattctg cagtcagttc cggcagttca
    19561 cgctcttcaa ctttcttcag gttgatagcg aatttcgctg ctttaccttt caggttttct
    19621 gcgtggtatt cttccgggaa ggtcacgtcg atggtgaact cttcgccagc tttgtggcct
    19681 ttgataccgt cttcaaagcc cgggatcata cgaccctggc ccatcgccag tacgaaatca
    19741 gacgctttac cgccttcgaa ctcttcgccg tctacagaac cggtgaagtc gatggttacg
    19801 cggtcttctg cttcaacagc gccgtctttt tctttccagg tcgcctgctg tttacgcaga
    19861 gtatccagca tgccgtcaac gtcagcgtcg gtcacttcaa cgatcggttt ttcaacttcg
    19921 atcgcttcca gaccctgcag ttcaacttcc ggataaactt caaactctac agagtaagtg
    19981 aagtcttcac ccagcttgta ttcgcccgga acataagtcg gtgcgccagc cggattgatt
    20041 ttttctttaa tgatggcgtc aatgaagtta cggctcatca ggtcacccag aacgtcctgg
    20101 cgtacagacg cgccataacg ctgagcaacg atattcattg gcactttgcc tttgcggaag
    20161 ccgtcaatac gtactttttt cgcaacgttg accagctcgc ttttaacagc ggtctcgatg
    20221 ctgtcagcag cgatagtaat cgttacacgg cggccaaggc cttgagtggt ttcaactgaa
    20281 acttgcatct tgttacctca aaaaatcaca gtgctcggtc aactcttacc ccgcaagcac
    20341 aggttgttgg cttcgcggaa ccgggatgtt cttataatca atcacattcc ctgttaccag
    20401 aatcatcccg aaagcattca aataggacgc ggcattatag cggcatcact tttatgagtc
    20461 gagaacggtt attgcgcgct gtcgcgtatt ttttcacaat tcccgggcaa ttttcgtctg
    20521 aatgctgggc aaaaagcgca aaatccagac aaaacaaaac ggcccgcagg ccgtttttca
    20581 taaaatcttc agcaacatac tggaaaagcg ccgacagttg caaatgcgtt tttacgcgat
    20641 gcttcctgct ccacgacagg gaggagaggc aaagacggtg tcctgcaacc cttcccactc
    20701 cttaatagtg taagtatgca gagccagcgc atgaacggta gtagagagtt cctccgctaa
    20761 agtactgtaa atcattcgat gacgattcag aaaacgttca cccgtaaaac gatcgctgac
    20821 cagcacaact ttaaaatggc tttcagagcc ggctgggaca ttgtgacgat agctttcatc
    20881 cactacttcg aggaatacgg gttggaacgc cgcccttaat ttttcttcta tccgctcacg
    20941 tatcatcatg aaatatctcc tccgacaacg ctgagatgtc atccatccct ttaaatacta
    21001 gccgctttta ccgtttccat tgcagcttaa caacaaatat ttaggtttcg tcctggtttt
    21061 tccgtcaagc gtatgaagtt tactgattta aagattgaaa cacaggcctc gcatcagcgt
    21121 ttcttttacc aggggtgatt atttcgtcaa catgatgaat ttacatgcct tacccacttc
    21181 ccctcgccgt tggcaatgtt atgatggcgg gaatttttac caaacacgct taagagtctc
    21241 tgagagcccg aacatgttca aaaaaatcct cttcccgtta gttgctctgt ttatgcttgc
    21301 aggatgcgca aaaccgccaa caactattga agtttccccg acgattacac tgccacagca
    21361 ggatccaagc ctgatgggcg tcaccgtaag cattaatggt gccgatcagc gtaccgatca
    21421 ggcgctggca aaagtcaccc gcgataatca aatcgttacc ctgaccgcct cccgcgatct
    21481 gcgtttcctg ctgcaagaag tgctggaaaa acagatgacc gcgcgtggtt acatggttgg
    21541 tccgaatggc ccggttaatc tgcaaatcat cgttagccaa ctgtatgctg acgtgtccca
    21601 gggcaacgtg cgctacaaca tcgctaccaa agcggatatc gccatcatcg ctaccgcgca
    21661 gaatggcaac aaaatgacca aaaactatcg tgccagctac aacgttgaag gtgcgttcca
    21721 ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg ctgaccgata ccatcgctga
    21781 tatgtctcag gacaccagca ttcacgaatt catcaagcag aacgcgcgtt aatttctgcc
    21841 ctctggcccg gtgcaagccg ggcctgtaga cgcccatgtc cagtcaatat ttacgtattt
    21901 ttcaacagcc gcgttcagcc atattgctga tcctgggttt tgcttccggg ctaccgctcg
    21961 ccctgacatc cggcacctta caggcctgga tgacggtcga gaatatcgat ctcaaaacca
    22021 ttggtttctt ctctctggta ggccaggctt acgtttttaa attcctctgg tcaccgctga
    22081 tggaccgcta cacgccccca ttttttgggc ggcggcgcgg ttggctgctc gccacgcaaa
    22141 tcctgttatt agtcgccatt gcggcgatgg gttttctcga accaggcacc caactccgct
    22201 ggatggcggc gctggcggtg gtgatcgctt tttgctctgc ctcccaggat attgtcttcg
    22261 atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg tgcgggcgcg gcaatcagcg
    22321 tgctgggtta ccgtttaggg atgctggttt ccggcggcct agccctgtgg ctggcagata
    22381 aatggttggg ctggcagggc atgtactggt tgatggcggc gctgttgatc ccctgtatta
    22441 tcgcgacgtt gcttgcacca gaaccaaccg acaccattcc tgtgcccaaa acgctggaac
    22501 aagcggttgt tgcacctctg cgagatttct ttggtcgcaa taatgcctgg cttattttgc
    22561 ttcttatcgt gctgtataag ctgggcgacg cattcgccat gagcctgaca accacgtttt
    22621 tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt ggtaaacaaa acgcttggct
    22681 tactggcgac cattgttggc gcattgtacg gtgggatttt gatgcagcgc ctgtcgctgt
    22741 tccgggcgct gctgattttc ggcattttac aaggtgcgtc taacgctggt tactggctgc
    22801 tgtcgattac tgataagcat ctctacagca tgggcgcagc cgtctttttc gaaaacctct
    22861 gtggcgggat gggcacatca gcctttgtcg cgctgttaat gacgctgtgt aataagtcat
    22921 tttccgctac tcaatttgcc ctgctctcag cgctttccgc tgtagggcga gtttatgtcg
    22981 gccccgtggc gggttggttt gttgaagcac acggctggtc gacattctat ctattctccg
    23041 tcgccgctgc cgtaccgggg cttattttgc tgctggtttg ccgccagacg cttgaatata
    23101 cacgagtaaa tgacaacttt atctcccgta ccgaatatcc ggcaggttat gcctttgcca
    23161 tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg gttacttctg ttgacgatgg
    23221 acgcgctgga tttgacgcac ttctctttcc tgcctgctct gctggaagtc ggggttttag
    23281 tcgccctttc tggcgtcgtg cttggtggtt tgctggatta tctggcgcta cgaaaaacgc
    23341 atctgacgta atctgtaaat attatttagc tgtagtaatc actcgccgta attattacgg
    23401 ctaaataaac atcagcatcg cttattaata tttgttcttt tgtgcggctt agcgtttgga
    23461 aattattggc gccatttata aattctattt ttcttacacg attcagctaa tgagtcttta
    23521 ttttctcacc acccagttgt cactctaatg ataattattt gttaaataat tgttttattt
    23581 cgcattggtt ataccaattg cccgccaaga ctccttctgc actccccttt tgttataacg
    23641 cccttttgca acagcttctt aaaatcaacc tgatatgttt tgcaacatat gtgacctgac
    23701 agccaaatct aagtaacagg aatttaacca tgtctacagt aatgtaacct ccccgtaaaa
    23761 tgcccacaca ctttaaacgc caccagatcc cgtggaattg aggtcgttaa atgagactca
    23821 ggaaatacaa taaaagtttg ggatggttgt cattatttgc aggcactgta ttgctcagtg
    23881 gctgtaattc tgcgctgtta gatcccaaag gacagattgg tctggagcaa cgttcactga
    23941 tactgacggc atttggcctg atgttgattg tcgttattcc cgcaatcttg atggctgttg
    24001 gtttcgcctg gaagtaccgt gcgagcaata aagatgctaa gtacagcccg aactggtcac
    24061 actccaataa agtggaagct gtggtctgga cggtgcctat cttaatcatc atcttccttg
    24121 cggtactgac ctagaaaacc actcacgctc ttgagcctag caagccgctg gcacacgacg
    24181 agaagcccat taccatcgaa gtggtttcca tggactggaa atggttcttc atctacccgg
    24241 aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc gaacactccg gtgtacttca
    24301 aagtgacctc caactccgtg atgaactcct tcttcattcc gcgtctgggt agccagattt
    24361 atgccatggc cggtatgcag actcgcctgc atctgatcgc caacgaaccc ggtacttatg
    24421 acggtatatc cgccagctac agcgggccgg gcttctcagg catgaagttc aaagctattg
    24481 caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa agcgaaacag tcgccgaaca
    24541 ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc tagcgaatac aaccaggtgg
    24601 aatatttctc caacgtgaaa ccagacttgt ttgccgatgt gattaacaag tttatggctc
    24661 acggtaagag catggacatg acccagccag aaggtgagca cagtgcacac gaaggtatgg
    24721 aaggcatgga catgagccac gcggaatccg cccattaaag gggttgagga agaataaaga
    24781 tgttcggaaa attatcactt gatgcagtcc cgttccatga acctatcgtc atggttacga
    24841 tcgctggcat tattttggga ggtctggcgc tcgttggcct gatcacttac ttcggtaagt
    24901 ggacctacct gtggaaagag tggctgacct ccgtcgacca taaacgcctc ggtatcatgt
    24961 atatcatcgt ggcgattgtg atgttgctgc gtggttttgc tgacgccatt atgatgcgta
    25021 gccagcaggc tcttgcctcg gcgggtgaag cgggcttcct gccacctcac cactacgatc
    25081 agatcttcac cgcgcacggc gtgattatga tcttcttcgt ggcgatgcct ttcgttatcg
    25141 gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg tgacgttgcg ttcccgttcc
    25201 tcaacaactt aagcttctgg tttactgttg ttggtgtgat tctggttaac gtttctctcg
    25261 gcgtgggcga atttgcgcag accggctggc tggcctatcc accgctatcg ggaatagagt
    25321 acagtccggg agtcggtgtt gattactgga tatggagtct ccagctatcc ggtataggta
    25381 cgacgcttac cggtatcaac ttcttcgtta ccattctgaa gatgcgcgca ccgggcatga
    25441 ccatgttcaa gatgccagta tttacctggg catcactgtg cgcgaacgta ctgattattg
    25501 cttccttccc aattctgacg gttaccgtcg cgttgttgac cctggatcgc tatctgggca
    25561 cccatttctt taccaacgat atgggtggca acatgatgat gtacatcaac ctgatttggg
    25621 cctggggcca cccggaagtt tacatcctga tcctgcctgt tttcggtgtg ttctccgaaa
    25681 ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac ctcgctggta tgggcaaccg
    25741 tctgcatcac cgtgctgtcg ttcatcgttt ggctgcacca cttctttacg atgggtgcgg
    25801 gcgcgaacgt aaacgccttc tttggtatca ccacaatgat tatcgccatc ccgaccgggg
    25861 tgaagatctt caactggctg ttcaccatgt atcagggccg catcgtgttc cattctgcga
    25921 tgctgtggac tatcggtttt atcgtcacct tctcggtggg cggtatgact ggcgtgctgc
    25981 tggcagtacc gggcgcggac ttcgttctgc ataacagcct gttcctgatt gcgcacttcc
    26041 ataacgtgat catcggcggc gtggtcttcg gctgcttcgc agggatgacc tactggtggc
    26101 ctaaagcgtt cggtttcaaa ctgaacgaaa cctggggtaa acgcgcgttc tggttctgga
    26161 tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct gggcttcatg ggcatgaccc
    26221 gtcgtttgag ccagcagatt gacccgcagt tccacaccat gctgatgatt gcagccagcg
    26281 gtgcagtact gattgcgctg ggtattctct gcctcgttat tcagatgtac gtttctattc
    26341 gcgaccgcga ccagaactgt gacctgactg gcgacccgtg gggtggccgt acgctggagt
    26401 gggcaacctc ttccccgcct ccgttctata actttgccgt agtgccgcac gttcacgaac
    26461 gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta taaaaagcct gaccactatg
    26521 aagaaattca tatgccgaaa aacagcggtg caggtatcgt cattgcagct ttctccacca
    26581 tcttcggttt cgccatgatc tggcatatct ggtggctggc gattgttggc ttcgcaggca
    26641 tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt ggattactac gtgccggtgg
    26701 cagaaatcga aaaactggaa aaccagcatt tcgatgagat tactaaggca gggctgaaaa
    26761 atggcaactg atactttgac gcacgcgact gcccacgcgc acgaacacgg gcaccacgat
    26821 gcaggcggaa ccaaaatctt cggattttgg atctacctga tgagcgactg cattctgttc
    26881 tcaatcttgt ttgctaccta tgccgttctg gtgaacggca ccgcaggcgg cccgacaggt
    26941 aaggacattt tcgaactgcc gttcgttctg gttgaaactt tcttgctgtt gttcagctcc
    27001 atcacctacg gcatggcggc tatcgccatg tacaaaaaca acaaaagcca ggttatctcc
    27061 tggctggcgt tgacctggtt gtttggtgcc ggatttatcg ggatgaaaat ctatgaattc
    27121 catcacctga ttgttaacgg catgggtccg gatcgcagcg gcttcctgtc agcgttcttt
    27181 gcgctggtcg gcacgcacgg tctgcacgtc acttctggtc ttatctggat ggcggtgctg
    27241 atggtgcaaa tcgcccgtcg cggcctgacc agcactaacc gtacccgcat catgtgcctg
    27301 agcctgttct ggcacttcct ggatgtggtt tggatctgtg tgttcactgt tgtttatctg
    27361 atgggggcga tgtaatgagt cattctaccg atcacagcgg cgcgtcccat ggcagcgtaa
    27421 aaacctacat gacaggcttt atcctgtcga tcattctgac ggtgattccg ttctggatgg
    27481 tgatgacagg ggctgcctct ccggccgtaa ttctgggaac aatcctggca atggcagtgg
    27541 tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa taccaaatca gatgaaggct
    27601 ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc tatcctggtt gtaggctcca
    27661 tctggattat gtggaacctc aactacaaca tgatgatgca ctaagagcgg cggttatgat
    27721 gtttaagcaa tacctgcaag taacgaaacc aggcatcatc tttggcaacc tgatctcggt
    27781 gattggggga ttcctgctgg cctcaaaggg cagcattgat tatcccctgt ttatctacac
    27841 gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg tttaacaact acatcgacag
    27901 ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg ctggttaaag gcctgatctc
    27961 tcctgctgtc tcgctggtgt acgccacctt gctgggtatt gctggcttta tgctgctgtg
    28021 gtttggcgcg aatccgctgg cctgctggct gggggtgatg ggctttgtgg tttatgtcgg
    28081 cgtctatagc ctgtacatga aacgccactc agtctacggc acattgattg gttcgctctc
    28141 cggcgctgcg ccgccggtga ttggctactg tgcagtaacc ggcgagttcg atagcggcgc
    28201 agcgatccta ctggctatct tcagcctgtg gcagatgcct cactcctatg ccatcgccat
    28261 tttccgcttt aaggattacc aggcggcaaa cattccggta ttgccagtgg taaaaggcat
    28321 ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc tttgccgttg ccacgctgat
    28381 gctctctctt ggcggttacg ctgggtataa atatctggtg gtcgccgcgg cggttagcgt
    28441 ctggtggtta ggtatggctc tgcgcggtta taaagttgct gatgacagaa tctgggcgcg
    28501 caagctgttc ggcttctcta tcatcgccat cactgccctc tcggtgatga tgtccgttga
    28561 ttttatggta ccggactcgc atacgctgct ggctgctgtg tggtaacaaa acctctctat
    28621 taaaaaggtg ctacggcacc ttttttattg tctgagatca gagatacggc taataaccgt
    28681 atctcttgac agagtgagca gatgcacata aattaatcat acggttgata gccgtattat
    28741 gtatgagatc gactccctac gatttcattc agttaatcaa agaagtcata atgcctggaa
    28801 aaacagccgc tctatacgat gtcgataaaa cgctgaaaaa tgcacgggtc gaactcaaaa
    28861 ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca agccgttggg gtagatttaa
    28921 gcgcttttat tttgagcgtt gctatggaac gcgccgaaag tgtgcttgat aaccaacgtc
    28981 gccgtgagct ttcgaatcaa agctgggaac tgatgaacca actcatcgct gaacctgctc
    29041 aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa cagcgatggt cgacaagcat
    29101 gaagagatta ctctgcccat agtcctctcc tgtaattatc agtctgatat tacttatcct
    29161 gggcaaaaac agtttgattg cggtaaccct gttatcgata aatttgtacg cgcatcgcta
    29221 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
    29281 gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc
    29341 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga
    29401 gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
    29461 gtaaaaaaat tcaccaggca ttaccaatta aaggggttta tcttgatgct aaccctgccg
    29521 ccattaactt ttatgctcgt ctcggctttg ttcagctttc agcgacacca aatgcttttg
    29581 gtgctgtacc tatgtttttg gcgattcagc atattctcgc ggcttagatt attatcgcca
    29641 cccgctaggg caagttttgc cttagccggg ggctgagcag cgttaccact tttgttcaat
    29701 tgcccccgct ttcagcaact ccacatcctt gccgttaata gttgccgttg cggcaacgat
    29761 tgtggtcctt acagttgcac aagattttcg ctcaaattcc ggtttataaa gaataagaga
    29821 cgaattactg gtaaatgaac caaataggta atcagtaaag ctaatattca gccggtttaa
    29881 accgaaaggc gcgaagccaa ccgtttggtt aatcttctta ccggatgggc gacgaatgct
    29941 gaatgtcacg cgaagattgg ccacttcgcc taatttattg gtggtggcga agaggctatc
    30001 catgcaggaa aaacctttat ctaaaaaatt atcccgcatc ccagaacggg taaccgtgat
    30061 cacgggtaac gtatcctcaa ccgtttttct gtcaatcatg tcttttcgac aagtagccag
    30121 aaagtcttcc gggccaccat aaatgtggac acctttatca aggtattcct tcgctttctt
    30181 acaatctacc tcaacatact gaccattggc gtacatcatg cccaacctgg accatgctaa
    30241 attcatacct tgctgcgcgg cctgattaaa tagtgcatac gctttggcat aatccacccg
    30301 cacctgctca ccgtcgtagt agatactccc cagcgctaac ggagcttctc tgtcaccttg
    30361 tttagcggct aactcgaacc atttaatcgc ttcctgaaca tcaggtttgg tatcatagcg
    30421 aggctgataa tattcccagc ctaacaggct ctgcgcgtcg atatctcccg attcagccat
    30481 tttatgcagt gtatttataa catcgggagc agatttacca ccgtatatgc tgtcatacaa
    30541 ttcatgcgct ttcttacttg ccaccacgcc tgatgaaact atcagcatta aagaaaataa
    30601 tattctatta ggcaacataa aatatctctt atttatcttg tacctgaata tgctcttctt
    30661 ttaataaatc acgttgtttt ccattcagta tcgctgtcgc agatttaata gaataagtca
    30721 aatcatgaaa atcgcataaa ttacgattat acattgggat gtctatatag ctttttctta
    30781 tatgactgga attattatac atatccacac cgagcgtatt caaactaaaa ggagggacag
    30841 aaagagtata ttctttactc actccctccc ccccctttta gctcaagcgt caactgcaaa
    30901 ttagttatac tactaatttt atctgcatta atttcaatac tgacattgca tattttgggt
    30961 ttttcatttg cgttaaagcc ataggtcccg taatgtttta acgtgatgta aggtaaagta
    31021 tcaccctgtg tttctcgggt tttctgatct tgattacaac gggttaaata ttcatcctta
    31081 ttgaatttca ttgaatattt ccccgcacct ttttcgtaat aatcccatac cttatcacaa
    31141 tcaagcgttt cacctaatcc gttgtaatac atccaactta accacaatgc ggcttttttt
    31201 gccccttg
//
LOCUS       DADKXM010000034        30578 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.034, whole genome shotgun sequence.
ACCESSION   DADKXM010000034 DADKXM010000000
VERSION     DADKXM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.034"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="JQR05_001541"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="JQR05_001541"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY5323737.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JQR05_001542"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JQR05_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY5323738.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="JQR05_001543"
     CDS             2341..2556
                     /locus_tag="JQR05_001543"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323739.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="JQR05_001544"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="JQR05_001544"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY5323740.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /locus_tag="JQR05_001545"
     CDS             4451..5845
                     /locus_tag="JQR05_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY5323741.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="JQR05_001546"
     CDS             5922..6419
                     /locus_tag="JQR05_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY5323742.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="JQR05_001547"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="JQR05_001547"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY5323743.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="JQR05_001548"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="JQR05_001548"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY5323744.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="JQR05_001549"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="JQR05_001549"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY5323745.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9032..9667
                     /locus_tag="JQR05_001550"
     CDS             9032..9667
                     /locus_tag="JQR05_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY5323746.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="JQR05_001551"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="JQR05_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY5323747.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="JQR05_001552"
     CDS             complement(10259..11239)
                     /locus_tag="JQR05_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY5323748.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="JQR05_001553"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="JQR05_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY5323749.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="JQR05_001554"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="JQR05_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY5323750.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQSTGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="JQR05_001555"
     CDS             12542..12832
                     /locus_tag="JQR05_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY5323751.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="JQR05_001556"
     CDS             12840..13433
                     /locus_tag="JQR05_001556"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY5323752.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="JQR05_001557"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="JQR05_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY5323753.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="JQR05_001558"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="JQR05_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..16387
                     /locus_tag="JQR05_001559"
     CDS             15884..16387
                     /locus_tag="JQR05_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY5323754.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            16387..17688
                     /locus_tag="JQR05_001560"
     CDS             16387..17688
                     /locus_tag="JQR05_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY5323755.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(17744..18751)
                     /locus_tag="JQR05_001561"
     CDS             complement(17744..18751)
                     /locus_tag="JQR05_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY5323756.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(18868..19914)
                     /gene="ansB"
                     /locus_tag="JQR05_001562"
     CDS             complement(18868..19914)
                     /gene="ansB"
                     /locus_tag="JQR05_001562"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY5323757.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(20090..20809)
                     /locus_tag="JQR05_001563"
     CDS             complement(20090..20809)
                     /locus_tag="JQR05_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY5323758.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(20993..21319)
                     /locus_tag="JQR05_001564"
     CDS             complement(20993..21319)
                     /locus_tag="JQR05_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY5323759.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGRICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            complement(21319..22038)
                     /gene="trmB"
                     /locus_tag="JQR05_001565"
     CDS             complement(21319..22038)
                     /gene="trmB"
                     /locus_tag="JQR05_001565"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY5323760.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            22199..23251
                     /gene="mutY"
                     /locus_tag="JQR05_001566"
     CDS             22199..23251
                     /gene="mutY"
                     /locus_tag="JQR05_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY5323761.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTSCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            23279..23554
                     /locus_tag="JQR05_001567"
     CDS             23279..23554
                     /locus_tag="JQR05_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY5323762.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            23619..24698
                     /gene="mltC"
                     /locus_tag="JQR05_001568"
     CDS             23619..24698
                     /gene="mltC"
                     /locus_tag="JQR05_001568"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY5323763.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            24900..26156
                     /locus_tag="JQR05_001569"
     CDS             24900..26156
                     /locus_tag="JQR05_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY5323764.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(26205..28340)
                     /locus_tag="JQR05_001570"
     CDS             complement(26205..28340)
                     /locus_tag="JQR05_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY5323765.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACSGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFVPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            28738..29445
                     /locus_tag="JQR05_001571"
     CDS             28738..29445
                     /locus_tag="JQR05_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY5323766.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            29551..29626
                     /locus_tag="JQR05_001572"
     tRNA            29551..29626
                     /locus_tag="JQR05_001572"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29584..29586,aa:Phe,seq:gaa)
     gene            30225..30515
                     /locus_tag="JQR05_001573"
     CDS             30225..30515
                     /locus_tag="JQR05_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323767.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
BASE COUNT         7457 a         7893 c         7532 g         7696 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aagctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact atcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ctgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gtcagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgt gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattat ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac acactctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactaacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgagc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcttgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag tcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tctgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctgga cggcgtgcgt
    13681 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 taaccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgccttg tgttttatta atcaaagcat tcaacaactg aaacaacctg cgcaggaggc
    16381 cagttaatgg atagttatat cgcgattaca ctgtttggct gtttttttgt gctggtcttt
    16441 attggtgtgc ccatttcgtt ctcaatcggt attgccactg tagcttcaat gcttttgatg
    16501 tttccctggg atatcgcggc aatcactgtt tcgcaacgac tggctaatgg actggataac
    16561 tttgccctgt tggcaatccc ctttttcatc tttgccggaa cactgatgaa cagcggcggt
    16621 attgcgatac gtttgattaa cctcgcccag gtaatggttg gccgcgttcc tggttcgctt
    16681 ggtcatgtga acgtcctggc gaatatgatg tttggttcca tttctggttc agccgttgcg
    16741 gcagctgctg ccgttggtgg cacactgaat cccattcaga ccaaagaagg ttatgatccg
    16801 gcattttcta ccgctgtaaa cgtctcatct tgtattactg gattactgat accgccctca
    16861 aacgtgttaa ttgtgttttc cctgacagct ggcggcgttt ctgtagcatc gctgtttatg
    16921 gcgggatatt tgccagggat cttaatgggt cttgcggtaa tgatagtctg cggcattatt
    16981 gctaaacgcc gtggctaccc gctttcagaa agagcaacct ttgctcaggc ctgtaaagcg
    17041 tttctggatg ccttaccaag tttgcttctg gtgttcattg tgatgggcgg cattctgggc
    17101 ggcattttta ccgcaactga agcgtcagca attgccgttg tctacacatt tattctctcg
    17161 gtgctgattt accgggaagt aaaatggcgc catttaccca aactgattct ggagtcggtt
    17221 gttaccactt cgattgtatt actgttgatc ggtttttccg tgggcatgtc ctgggcaatg
    17281 actaatgctg atattcctta tatgatcagc gacgcattga tggggatttc tgataaccca
    17341 ctgatcatat tgctgctgat taatatcgtc ttattaattg tcggcatctt tatggacatg
    17401 acaccggctg tactaatctt tacaccgatc ttcttgccga ttgcacaaga gctgggaatg
    17461 gacccagtac atttcggtat tatgatggtg gccaaccttt gtattgggct gttaacaccg
    17521 ccggtgggta gtgcgctatt tgttggttgt tctatctcgg gtgtgaagat tcaacattta
    17581 atcaaaccat tgctgccatt ttatgctgca ttgctagtag cgttgatgat gatcacttac
    17641 attccgcaaa tatccttgtt cattccacag ctactagggc taatgtaaaa aataatttgc
    17701 cggataactt tttatattat ccggcaaagg cacttaacat gagttactta atcaccccat
    17761 tgcgccgcag ttgttcggta tcttttttcc agcgttcatt agcttggttg atatcgtcaa
    17821 ttttcatcaa aatcgcgact tttttactgt ttatttctga aactttcatc gttaacggat
    17881 attgcccttt ttcgagcgtt acccagtcat cctgcaattt acggctgagt ttttgtgatt
    17941 gcagaaaagt ctgtcgccgt tgttcgttga tatcgaaatc tcgtttctgc tgaatcggca
    18001 cttcataaca ctcttcactc tgtttatgac atgccgcaat cgccggttga taaacctctt
    18061 tgtcatattt aaccgcgaac gcctcgctat cgttctgttt attaaactca tcacgctgtt
    18121 ggtgaatttt tttaaaggct tcttcgcggg tcatttgctt accgtttgca tcttttcccc
    18181 aatatgcatc cgctgctttc tgcgccgcca ccatttgctt atcaagcgct ttcacttcct
    18241 tttccagcgc aggaatcgag ttcttctggg tattcaacgc agattcaagc tgactaaagc
    18301 gataattgaa atcttcgcta ttcacgatca gatatttatt attcgttttg atatcgtcga
    18361 ccacccgccc acgatcgctg gctgccgcct gaaaagagta aaagttcacc gaccagccag
    18421 acgcgggcgt tcctttactg gttaacatcg ccgagaattt taccggttta tctttcgtcc
    18481 aggtctgttc gagcagttcg taatctgcca actgaccgac ccaggtatag agatcgtcac
    18541 tggaagagac atcgccttcc gctgaccagg tggcctgatt acccttagca tcaagatttt
    18601 ttaagctgat ggcatcaagc ttgagaatgc cgtgatactg ctttttgaat tgatctttta
    18661 agatattttc agttggcggt tcgccatctg cccaggcatt accagtcatc aacataagca
    18721 gcgccgtgcc aacaatccat tgctttttca cccgaaaacc ctcgcgcgga ataatttatt
    18781 ccggtgatta tcattagggt aatgaaaatt ggcaatggac gtaagtctga agtgaaaaag
    18841 ccccggcacg atgccgggac gaggtgatta gtactgattg aaaatctgct ggatctgctg
    18901 cggatctttg gtttgcgtca gagccagctg cagcaggacg cgcgcttttt gcgggttcag
    18961 cgtgccagag gcgacgaagc cgtatttcgc atcatccact tcagcatcct gagtggtagc
    19021 acccgtcggt acgcgggaag aacgcactac tgcagtgccg tttttcgcgg cggttgccag
    19081 ggtgtcgaac acggatttat acaggttacc attacccaca ccagcgctaa cgatgccatc
    19141 atagcccgca tctaccagtg ctttagccgg aagatcggat gcgttagcgt agttataaac
    19201 gatgccgact ttcggcagct cattcagctt agagacatcg aatggcgtat cgctggtgtg
    19261 cttacgtgcc ggggtacgtt ggtagtcaat cttaccgttg tgaatgtatc ccagaggacc
    19321 gtagttaaca gacttgaagg tcgctacgtc ggtggtgttg gttttggtga catcgcgacc
    19381 gtccagtacg gtgtcgttca tcaccaccag cacgccacga ttagcggagg ctttatcagc
    19441 tgcggtcact accgcgttat acaggttgaa tggaccgtct gcgctcatgg acgtggacgg
    19501 gcgcattgcg ccgaccatca ccaccggttt gtcgcatttc accgtcaggt cgaggaagta
    19561 agcagtttct tccatcgtgt cggtaccgtg ggtaatgacg aagccgtcgg ttttatcgca
    19621 gtcggtgtta atttttttcg ccagtgtcag ccagacatta tcgttcatgt cctgggagcc
    19681 gatattcact acctgctcgc ctttaacgtt cgcaatgtct tttagttgcg gcaccgcatt
    19741 aaccagattt tctacgccaa ctttacccgc tgtgtagtta gatttggttg cggagtcacc
    19801 accaccggca atggtcccgc cggttgctaa aatggtgata ttgggtaatg ccaatgctgc
    19861 accactaaaa cccataacca gtgcggcaag agccgtcttt ttgaaaaact ccatttcatt
    19921 cctccagtta cgtgaacgct acgcattatc ccttagcttt gtatgggaaa tttgacgtta
    19981 aacaatttac aacgtgaata tattttggag atctacaaag ttagaggcag gtaacaaaac
    20041 gaagaattaa acggcataaa aaagtattat gccgtcttaa aatagaggat tattttaaat
    20101 tcccgaccag ggctttgcgg ctatcttcca gagtcacaac gcggctacaa acatctttgc
    20161 caaactgctg gaaatctttt tcctgctttt tccactcggt ttggattgag gattgcagac
    20221 cgcccaagct tcccagcaca ttctgtaatg ggttaccgcc gcttttcagc accgctttcg
    20281 cgcccatttc attaatgctg tcctgtaaaa ttccgcccat tgcctgattc actagttgct
    20341 ggccttcggc gcgaacctga tcaatggctt tatagtggaa cgtcaggcca tcgctgcgcg
    20401 tttcgataat gcgattcatc tgctctttaa gctgcgcatc aagtttggtc agacggctgc
    20461 gcattttgct gctgtcgccc atctcctgaa cgataatttt atccagcgca atacgggctt
    20521 tctcgacgcg gcttttcgcg ccttcatcaa tccacggcag cgtgctacgt agttcagcct
    20581 gataatcctt cgcctgctcg cgctgggcgg cattcaggga atattgctta ccgttataca
    20641 tcacgttgcc gtctggcgtg atcaccagat tgccgttttc gcccttcacc tgcacggttt
    20701 gcgggctgac aatcacatcg tcacgcggcg tgacgctgca ctggtagtcg gcgtgagcgg
    20761 tcattgccgt cactgaaagt gctgccgcca gcagcatttt gcgcatcata gtcttccctc
    20821 aagaacaaat caggccagca tatgctggcc ccagattgat aacaaagtac gcgttttcca
    20881 tgccggatgc ggcgtaaacg ccttatcccg cctactcgat cgtgcatctc tgtaggcctg
    20941 ataagcgtag cgcatcaggc agttttgcat ttgtcatcgc ccgtatgctt tcttagtccc
    21001 accaaacgtc gaaaagttcg ctggtgcgta cctcatccag tttgcactct tccagccact
    21061 tacgcacaat cgcctgatgt tcttcggtgc atttgccgat ttcctgcatg cagatccgac
    21121 cttcccaggc cagataaccg ctgccgtcaa aggccagttt gttcggttcg ataacctcgt
    21181 taataaagtc atcaacgatt ttatcaatct gttcttccga tgtaccttcc gggaatcgcc
    21241 atgccaccga aaatcctaat tcctggaatt cgtcgatgtg cattttttta cgcagacgac
    21301 ggctacggtt ctttgccatt atttcaccct ctcgaacatt aagtcccata ctccgtgacc
    21361 aagacgatga ccacgttgtt caaatttcgt caccggacgt gatgccggac gcggtacgta
    21421 atcattgctc tctgacaggt ttttataacc gtcaatagaa gacatcactt caagcatatg
    21481 ttccgcataa ggttcccagt cggtcgccat atggaatacg ccccccagct gcagtttgct
    21541 ttttaccagt tcggcaaacg gcacctgaac gatacggcgt ttattatggc gcgctttgtg
    21601 ccacgggtca gggaaaaaga gctgcaccat gcgcaatgaa ttgtcaggaa tcattttatg
    21661 cagcacttca accgcatcgt gacacatcac gcgcaggttg cttaaacctt cttcatgcgc
    21721 agaagccagg cacgcaccaa cgcccggtga atgcacttca atgccgagga agtcctgctc
    21781 ggggcgatct ttagccattg ccaccagcga cgcccccatg ccaaaaccaa tctcaagcgt
    21841 caccggcgct tcacggccaa aaagcgcggg gaaatccagc atatcttcac tgaactcaac
    21901 gcccatcacc ggccagtagt tttccagcgc atgttcctgg cctttggtca gtcgcccctg
    21961 gcggcgcaca aaactacgga tacggcgcag tgggcggccg ttttcatcaa attccggtga
    22021 aatgacgtcg tttttcataa agatttagtc gcttgtgaaa gtgttctgaa aacgggcatt
    22081 atccaaagtt agttgccgga tgcaagcatg ataaggccgt ggctgcggaa agttccggtt
    22141 tacaccctgc cgtcgctgtg ctgcaatctt gcccccaaca acagtgaagt cggtgaccat
    22201 gcaagcgtcg caattttcag cccaggttct ggactggtac gataaatacg ggcggaaaac
    22261 gctgccctgg caaattgaca agacgcccta caaagtatgg ctctcagaag tgatgttgca
    22321 acaaactcag gttgcgaccg ttatccccta ttttgaacgc tttatggcgc acttcccgac
    22381 ggtgaccgat ctcgccaatg cgccgctcga cgaagttctc cacttgtgga ccgggcttgg
    22441 ctattacgcc cgcgcgcgca acctgcataa agcggcacaa caagtggcga ccttacacgg
    22501 cggtaaattc ccggaaacct ttgaagaagt cgcggcgtta ccgggcgtcg ggcgttccac
    22561 cgcaggcgcg attctctcgc tttctctggg taagcacttt ccgattctcg acggtaacgt
    22621 caaacgcgtg ctggcgcgct gctatgctgt aagcggctgg cctgggaaaa aagaggtcga
    22681 gaataaacta tggagtttga gcgagcaggt gacgcccgcg gtcggcgtgg aacggtttaa
    22741 tcaggcgatg atggatttgg gcgcgatgat ttgcacgcgt tcgaagccga aatgttcgct
    22801 ctgtccgcta caaaacggat gtattgccgc caccaacaat agctggtcgc tttatccggg
    22861 caaaaaaccg aaacagacgc tgccggagcg caccggctac tttctcctgt tacagcacga
    22921 agatgaagta ttgctggcgc agcgtccgcc gagcggattg tggggcggtt tatactgttt
    22981 cccgcagttt gccgacgaag aaagtttgcg gcattggctg gcacaacggc agattgctgc
    23041 cgataacctg acgcaactga ccgcgtttcg ccataccttc agccatttcc acttagatat
    23101 tgtgcctatg tggcttcccg tgtcgtcatt caccagctgc atggatgaag gcaatgcgct
    23161 ctggtataac ttagcgcaac cgccgtcagt tggcctggcg gctcccgtgg agcgtttgtt
    23221 acagcagtta cgcactggcg cgccggttta gcgcgtgagt cgataaagag gatgatttat
    23281 gagcagaacg attttttgta ctttcctgca acgtgaagca gaaggtcagg attttcagct
    23341 gtaccccggc gagctgggaa aacgcatcta taacgagatc tccaaagaag cctgggcgca
    23401 gtggcagcac aaacaaacca tgctgattaa tgaaaagaaa ctcaacatga tgaatgccga
    23461 gcaccgcaag ctgctggagc aggagatggt caacttcctg ttcgagggta aagaggtgca
    23521 tatcgagggc tatacgccgg aagataaaaa ataaaaacag tgccggagca cgcctccggc
    23581 agcttgcata aaaacaaaca caacacgcac ccggaatgat gaaaaaatat ctcgcgctgg
    23641 ctttgattgc gccgttgctc atctcctgtt cgacgaccaa aaaaggcgat acctataacg
    23701 aagcctgggt caaagatacc aacggttttg atattctgat ggggcaattt gcccacaata
    23761 ttgagaacat ctggggcttc aaagaggtgg tgatcgccgg tcctaaggac tacgtgaaat
    23821 acaccgatca atatcagacc cgcagccaca tcaacttcga tgacggtacg attactatcg
    23881 aaaccatcgc cgggacagaa cctgccgcgc atttgcgccg ggcaattatc aaaacgttgc
    23941 tgatgggtga cgatccgagt tcggtcgatc tctattccga cgttgatgat attacaattt
    24001 cgaaagaacc tttcctttac ggtcaggtgg tggacaacac cgggcagccg attcgctggg
    24061 aaggtcgcgc aagcaacttc gcggattatc tgctgaaaaa ccgtctgaaa agccgcagca
    24121 acgggctacg tatcatctac agcgtcacca ttaacatggt gccgaaccac cttgataaac
    24181 gtgcgcacaa atatctcggc atggtccgcc aggcgtcacg gaaatatggc gttgatgagt
    24241 cgctgattct ggcaattatg cagactgaat cttcctttaa cccgtatgcg gtcagccgtt
    24301 ccgatgcgct gggattaatg caggtggtac aaaatactgc cgggaaagat gtgttccgct
    24361 cgcaggggaa atccggcacg ccgagccgca gtttcttgtt tgatcctgcc agcaatattg
    24421 ataccggcac cgcgtatctg gcgatgctga acaatgttta tctcggcgga attgataacc
    24481 caacatcgcg gcgttatgcc gtcatcaccg cctataacgg cggcgcaggc agcgtgctgc
    24541 gagtcttttc gaatgataag attcaggctg ccaatattat taacaccatg acgccgggcg
    24601 atgtttatca gacgctgacg acccgccatc cctctgcgga atctcgccgt tatctttata
    24661 aagtgaatac cgcgcaaaaa tcctaccgcc gccgataatt ccattaaccg cccctgacga
    24721 tgctcagggg caaaaatgtt atccacatca caatttcgtt ttgcaaattg ggaatgtttg
    24781 caattatttg ccacaggtaa caaaaaacca gtccgcgaag ttgatagaat cccatcatct
    24841 cgcacggtca aatgtgcttt ttcaaacact catccgcatc acgatgtgag gaaattaaca
    24901 tgaatcttaa gctgcagctg aaaatcctct cttttctgca gttctgtctg tggggaagtt
    24961 ggctgacgac cctcggctcc tatatgtttg ttaccctgaa gtttgacggt gcttctattg
    25021 gcgcagttta tagctcactg ggtatcgccg cggtatttat gcctgcgctg ctggggattg
    25081 tggccgacaa atggttaagt gcgaaatggg tatatgccat ttgccacacc attggcgcta
    25141 tcacgctgtt catggcggca caggtcacga cgccggaagc gatgttcctt gtgatattga
    25201 ttaactcgtt tgcttatatg ccaacgcttg ggttaatcaa caccatctct tactatcgcc
    25261 tgcaaaatgc cgggatggat atcgttactg acttcccgcc aatccgtatc tggggcacca
    25321 tcggctttat catggcaatg tgggtggtga gcctgtctgg cttcgaatta agccacatgc
    25381 agctgtatat tggcgcagca ctttccgcca ttctggttct gtttaccctg actctgccgc
    25441 atattccggt tgctaaacag caagcgaatc agagctggac aaccctgctg ggcctcgatg
    25501 cattcgcgct gtttaaaaac aagcgtatgg caatcttctt catcttctca atgctgctgg
    25561 gcgcggaact gcagattacc aacatgttcg gtaatacctt cctgcacagc ttcgacaaag
    25621 atccgatgtt tgccagcagc ttcatcgtgc agcatgcgtc aatcatcatg tcgatttcgc
    25681 agatctctga aaccctgttc attctgacca tcccgttctt cttaagccgc tacggtatta
    25741 agaacgtaat gatgatcagt attgtggcgt ggatcctgcg ttttgcgctg tttgcttacg
    25801 gcgacccgac tccgttcggt actgtactgc tggtactgtc gatgatcgtt tacggttgcg
    25861 cattcgactt cttcaacatc tctggttcgg tgtttgtcga aaaagaagtt agcccggcaa
    25921 ttcgcgccag tgcacaaggg atgttcctga tgatgactaa cggcttcggc tgtatcctcg
    25981 gcggcatcgt gagcggtaaa gttgttgaga tgtacaccca aaacggcatt accgactggc
    26041 agaccgtatg gttgattttc gctggttatt ccgtggttct ggccttcgcg ttcatggcga
    26101 tgttcaaata taaacacgtt cgtgtcccga caggcacaca gacggttagc cactaattac
    26161 gcaaagaaaa acgggtcgcc agaaggtgac ccgttttttt attcttactt caacacataa
    26221 ccgtacaatc gtttcatgcc atccgcatcg gtttcgctat agacaccttg caactccggc
    26281 gaaaatcccg gcaacaaatt cacgccttct tccagcgcaa ggaaataacg ttgaaccgcc
    26341 ccaccccaga cttccccggg taccacgcaa agcacgccag gtggataagg caacgcccct
    26401 tctgccgcaa ttcgcccttc ggcatcacga atccgcacca actccacttc accgcgaata
    26461 taagcgctat gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac
    26521 atcgcttttt gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac
    26581 aactggcgca gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac
    26641 acctcagcca gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc
    26701 agttgtgcca gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga
    26761 tcgcacttct ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga
    26821 acgccaaagt cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc
    26881 ttgcacggat caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc
    26941 cccggcacaa aactgaaaaa acggcggtcg ctggctaaca ctgatgtcgg ataatcctgc
    27001 cacaatttgc catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga
    27061 atagccttgc gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc
    27121 tccccttcat gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg
    27181 gtagaagcat ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt
    27241 ccgcggatat ggttatcttt tttatggatc tgcgacgtct gtgagaatcc agcctgctgt
    27301 ttgtgcacca actgtgtcac aaaaatcccc ggatcgtttt cgttaagttc taacagcagc
    27361 ggcgagctat ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac
    27421 agaatgtaat cacacagatg cccaatggta tcgatcacct gacgggcgtt atagacagtg
    27481 ccgtcatagg ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag gtcggctttt
    27541 tctggcgcaa cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca
    27601 ataccgccaa tgaaaccaaa cgggttacgt gaagcttcca gatagaccgg cgtcgtcccc
    27661 gcctgaatca gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga
    27721 tcgccacgcg ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc
    27781 acaaaatagg ttttatcggc atgaaagact ttggctgcga atttctgcgc atctttcgcc
    27841 gatccttcat gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga
    27901 aagacgttct caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaagaac
    27961 gcaccatgtt gatgtccaga gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc
    28021 agcgtgtcat aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc
    28081 tccagccact gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca
    28141 gcatgttcgg aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga
    28201 atgccactgc gactatccgc agcggtaatg acgactgccg cgacgtccgt aaaatcagta
    28261 tctcccaacg ccaccacgcg gcgatgagaa gaaagtcggg ataccagttc actactggcg
    28321 gcaatattca ttgatttcat aagcgcaaac ccgtttcggg gaagtaagaa taccggacaa
    28381 ggtggaaaac cctgccccat gagatatggg tcaaactggt caccagctcc gaccgccaga
    28441 catcagtaaa agcagaaacg ctctgatttt actgttgtcc tgcagtgagc gtgcgttaac
    28501 ttcaccgcat gagcagtaac atagaaaggg aaacgtttcg cgcgaaacgg cgataagcga
    28561 gagaatgtaa ggagatggcg tgcatcgggc aaactccgta cagagaggag aaaattcgcg
    28621 caatcatggc acctttcgct aaggcgtgca agccaggact ttgcgaccag acaagccatc
    28681 aacaaggcaa aaagttcata ataagaaaat caaacaatac agctaacagg aactcttgtg
    28741 gtcatcggcc cttttatcaa cgctagtgca gtcttactgg gtggcgttct cggcgcactg
    28801 ctcagccaac gcttaccgga acgtatccgc gtctccatga catctatttt tggtctggca
    28861 tcgctgggga ttggtatttt actggttgtg aaatgcgcca accttccagc gatggtttta
    28921 gctacgctac ttggcgctct aattggcgaa atttgtttgc tggagaaagg tgtcaataca
    28981 gcagtcgcca aagcacaaaa tctgtttcgt cactcacgta agaagccagc gcatgaatct
    29041 tttattcaga attatgtcgc gattattgtc ctgttttgcg ccagcggcac cgggatcttc
    29101 ggggcgatga acgaagggat gaccggcgat ccgagtattt taatcgccaa gtcatttctt
    29161 gatttcttta cggcgatgat cttcgcctgc tcgctgggta ttgcggtgtc ggtaattagt
    29221 atcccattac tgatcatcca gttaacgctg gcgtgggctg ccgcgctgat attaccgctg
    29281 accacaccgt cgatgatggc agacttcagc gccgtaggcg gtttattgct gctggcaacc
    29341 ggattacgca tctgtggcat taaaatgttc ccggtggtca acatgcttcc agcactcctt
    29401 ctggcaatgc cgctttccgc cgcctggacc gcctggtttg cctgacaatg cgtgcaatag
    29461 cggcaaagtg atgatagatt gtgcagtctg cagtaaattg aagaaatttg attgacgaga
    29521 cgaggcgaat caggtttaat gcgccccgtt gcccggatag ctcagtcggt agagcagggg
    29581 attgaaaatc cccgtgtcct tggttcgatt ccgagtccgg gcaccactat ttagaagaac
    29641 cagcctacgg gctggttttt tgctttctgg ggttctggta gatatccggt cactttgaat
    29701 gctgatgtca ctgctttacc tcttccatcc agcctgaaca tttcttctaa gtagccccct
    29761 tctggtcact gtggcctttt ctgtcaaact tcaaatcccc aatagacact atttccacat
    29821 gagcacctgg ctaacggatt cattactgca cggatacatc cggtctttgt ccggtctgaa
    29881 gtaaaacgca taccacctgc gttggcccct cctcagtccc ccctacatca aaccgaacat
    29941 ttttcactct gattgattta gctttagtca acttttgggt gttatgccta ctatatcaat
    30001 atgatccata ggggattatg gccagactac cggtaatacc gagagacagg atctcaatcg
    30061 gagatagaac gttctgcaaa acggggctta cagaaaatcc ctaaatgacc tgcaacctcc
    30121 agcccattgc atattatgat aacagtgaat attttggtta agtcgcaccc cgcaaaaggt
    30181 ggaaagcaat ttaacaaatt gaattaaaag ggtttaaccg caaaatggac gcaacaggca
    30241 acacgacagt cacctgcctg agtcacaaaa tgaagtacaa agaagtcgcc tgcggtaagc
    30301 agcggatgca ctggctggcg ctgcagttcg tggatgagaa caacaaccca gtttccggtc
    30361 tcaacgtgca gctggagtat catccacggg caatggcggc agagctggct ctgtgggcca
    30421 gaggcagaca cactcagttt gaccccacac cgccgccccc acctgtcggt gtcactgaca
    30481 gtcagggtct ggtacggttt gatgacttgt actgaattaa agcacctaaa acaaacattt
    30541 atatcgcaac agttaaatag aatggataat cggtaata
//
LOCUS       DADKXM010000035        30235 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.035, whole genome shotgun sequence.
ACCESSION   DADKXM010000035 DADKXM010000000
VERSION     DADKXM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.035"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            288..671
                     /gene="secE"
                     /locus_tag="JQR05_001574"
     CDS             288..671
                     /gene="secE"
                     /locus_tag="JQR05_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY5323768.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            673..1218
                     /gene="nusG"
                     /locus_tag="JQR05_001575"
     CDS             673..1218
                     /gene="nusG"
                     /locus_tag="JQR05_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY5323769.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1377..1805
                     /gene="rplK"
                     /locus_tag="JQR05_001576"
     CDS             1377..1805
                     /gene="rplK"
                     /locus_tag="JQR05_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY5323770.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1809..2513
                     /gene="rplA"
                     /locus_tag="JQR05_001577"
     CDS             1809..2513
                     /gene="rplA"
                     /locus_tag="JQR05_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY5323771.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2926..3423
                     /gene="rplJ"
                     /locus_tag="JQR05_001578"
     CDS             2926..3423
                     /gene="rplJ"
                     /locus_tag="JQR05_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY5323772.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3490..3855
                     /gene="rplL"
                     /locus_tag="JQR05_001579"
     CDS             3490..3855
                     /gene="rplL"
                     /locus_tag="JQR05_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY5323773.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4175..8203
                     /gene="rpoB"
                     /locus_tag="JQR05_001580"
     CDS             4175..8203
                     /gene="rpoB"
                     /locus_tag="JQR05_001580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY5323774.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLGLGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8280..12503
                     /gene="rpoC"
                     /locus_tag="JQR05_001581"
     CDS             8280..12503
                     /gene="rpoC"
                     /locus_tag="JQR05_001581"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY5323775.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12745..13878)
                     /gene="thiH"
                     /locus_tag="JQR05_001582"
     CDS             complement(12745..13878)
                     /gene="thiH"
                     /locus_tag="JQR05_001582"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY5323776.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13875..14720)
                     /gene="thiG"
                     /locus_tag="JQR05_001583"
     CDS             complement(13875..14720)
                     /gene="thiG"
                     /locus_tag="JQR05_001583"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY5323777.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14647..14847)
                     /gene="thiS"
                     /locus_tag="JQR05_001584"
     CDS             complement(14647..14847)
                     /gene="thiS"
                     /locus_tag="JQR05_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY5323778.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14831..15586)
                     /gene="thiF"
                     /locus_tag="JQR05_001585"
     CDS             complement(14831..15586)
                     /gene="thiF"
                     /locus_tag="JQR05_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY5323779.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15579..16214)
                     /gene="thiE"
                     /locus_tag="JQR05_001586"
     CDS             complement(15579..16214)
                     /gene="thiE"
                     /locus_tag="JQR05_001586"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY5323780.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16214..18109)
                     /gene="thiC"
                     /locus_tag="JQR05_001587"
     CDS             complement(16214..18109)
                     /gene="thiC"
                     /locus_tag="JQR05_001587"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY5323781.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYSDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18190..18290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18343..18819)
                     /gene="rsd"
                     /locus_tag="JQR05_001588"
     CDS             complement(18343..18819)
                     /gene="rsd"
                     /locus_tag="JQR05_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY5323782.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18914..19687
                     /gene="nudC"
                     /locus_tag="JQR05_001589"
     CDS             18914..19687
                     /gene="nudC"
                     /locus_tag="JQR05_001589"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY5323783.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19727..20791
                     /gene="hemE"
                     /locus_tag="JQR05_001590"
     CDS             19727..20791
                     /gene="hemE"
                     /locus_tag="JQR05_001590"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY5323784.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLIGRD
                     YQQFSLYYMHKIVDGLLRENDGRCVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20948..21619
                     /gene="nfi"
                     /locus_tag="JQR05_001591"
     CDS             20948..21619
                     /gene="nfi"
                     /locus_tag="JQR05_001591"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY5323785.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21662..22252
                     /locus_tag="JQR05_001592"
     CDS             21662..22252
                     /locus_tag="JQR05_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY5323786.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22439..22711
                     /gene="hupA"
                     /locus_tag="JQR05_001593"
     CDS             22439..22711
                     /gene="hupA"
                     /locus_tag="JQR05_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY5323787.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22724..23419
                     /locus_tag="JQR05_001594"
     CDS             22724..23419
                     /locus_tag="JQR05_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY5323788.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23421..23846)
                     /gene="zraP"
                     /locus_tag="JQR05_001595"
     CDS             complement(23421..23846)
                     /gene="zraP"
                     /locus_tag="JQR05_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY5323789.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24084..25460
                     /gene="zraS"
                     /locus_tag="JQR05_001596"
     CDS             24084..25460
                     /gene="zraS"
                     /locus_tag="JQR05_001596"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY5323790.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     MRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25457..26782
                     /gene="zraR"
                     /locus_tag="JQR05_001597"
     CDS             25457..26782
                     /gene="zraR"
                     /locus_tag="JQR05_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY5323791.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCATLNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26779..28068)
                     /gene="purD"
                     /locus_tag="JQR05_001598"
     CDS             complement(26779..28068)
                     /gene="purD"
                     /locus_tag="JQR05_001598"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY5323792.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28080..29669)
                     /gene="purH"
                     /locus_tag="JQR05_001599"
     CDS             complement(28080..29669)
                     /gene="purH"
                     /locus_tag="JQR05_001599"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY5323793.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLTIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVSGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7091 a         7873 c         8315 g         6956 t
ORIGIN      
        1 aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgagctaatt
       61 gccgataaca tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg
      121 ctttcgtacc agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag
      181 gctctgttga gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc
      241 gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata
      301 ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat
      361 tgctcctggt ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg
      421 cgctggccgt agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag
      481 gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc
      541 cgactcgcca ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt
      601 cactgatcct gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc
      661 tgaggttctg agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc
      721 ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa
      781 gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag
      841 cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac
      901 gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact
      961 tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag
     1021 caggttggtg ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt
     1081 aatgatggtc cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct
     1141 cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc
     1201 caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc
     1261 gtgagattgg aatacaattt tgcgcctttt gtttttatgg gccttgcccg taaaacgatt
     1321 ttttatatca cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg
     1381 ctaagaaagt acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc
     1441 cgccagtagg tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt
     1501 tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt
     1561 acgctgaccg ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa
     1621 aagcggctgg tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt
     1681 cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca
     1741 ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg
     1801 actaagaaat ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa
     1861 ccaaacagta cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat
     1921 tcgtagaaag cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga
     1981 acgtacgtgg tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat
     2041 ttacccaagg tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg
     2101 aagatctggc tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc
     2161 cggatgcaat gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc
     2221 caaacccgaa agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag
     2281 ctggccaggt tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag
     2341 tggactttga cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa
     2401 aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca
     2461 tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt
     2521 acgtgggagg tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta
     2581 tatccgagat attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct
     2641 aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga
     2701 tagaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag
     2761 tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta
     2821 tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag
     2881 tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt
     2941 caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca
     3001 gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt
     3061 cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa
     3121 ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct
     3181 atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa
     3241 tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc
     3301 ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa
     3361 gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct
     3421 taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga
     3481 acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta
     3541 atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct
     3601 gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta
     3661 attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact
     3721 ggcctgggtc tgaaagaagc taaagacttg gtagaatctg caccggctgc tctgaaagaa
     3781 ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa
     3841 gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg
     3901 tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt
     3961 gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga
     4021 cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg
     4081 aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca
     4141 gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa
     4201 acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc
     4261 tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct
     4321 ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca
     4381 atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg
     4441 cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc
     4501 gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct
     4561 catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct
     4621 gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa
     4681 agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga
     4741 tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat
     4801 tctgcgcgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat
     4861 ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga
     4921 aaccgcatct tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat
     4981 cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt
     5041 tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct
     5101 gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg
     5161 tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga
     5221 aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat
     5281 gatgcgccct ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt
     5341 cttctccgaa gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct
     5401 gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat
     5461 gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg
     5521 caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt
     5581 acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc
     5641 tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag
     5701 ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg
     5761 tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga
     5821 cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat
     5881 cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac
     5941 tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat
     6001 cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt
     6061 cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca
     6121 ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc
     6181 gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc
     6241 cgttccgact ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc
     6301 cgttgactcc ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc
     6361 ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga
     6421 catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc
     6481 gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac
     6541 cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg
     6601 ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac
     6661 caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga
     6721 gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg
     6781 tatcgtttac attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc
     6841 gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa
     6901 agcctctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat
     6961 cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga
     7021 agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc
     7081 gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct
     7141 cgacaaactg ccgcgcgatc gctggctggg gctgggcctg acagacgaag agaaacaaaa
     7201 tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact
     7261 cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat
     7321 tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg
     7381 tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga
     7441 aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat
     7501 cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa
     7561 cgccatgctg aaacagcagc aagaagttgc gaaactgcgc gaattcatcc agcgtgcgta
     7621 cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt
     7681 tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg
     7741 tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca
     7801 gatccgcctg tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta
     7861 catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg
     7921 ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg
     7981 tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga
     8041 aatgctcact gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt
     8101 ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga
     8161 gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt
     8221 cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg
     8281 tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca
     8341 aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc
     8401 cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta
     8461 tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc
     8521 gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc
     8581 gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc
     8641 cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact
     8701 ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga
     8761 ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg
     8821 gtgcggaagc aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc
     8881 tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta
     8941 tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg
     9001 ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg
     9061 cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac
     9121 gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg
     9181 aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca
     9241 agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga
     9301 acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc
     9361 tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca
     9421 tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg
     9481 ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg
     9541 tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac
     9601 tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact
     9661 tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc
     9721 gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg
     9781 ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca
     9841 aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc
     9901 tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg
     9961 gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga
    10021 tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa
    10081 tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg
    10141 cggaccagat catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca
    10201 tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag
    10261 ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca
    10321 aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc
    10381 tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca
    10441 gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg
    10501 ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg
    10561 cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta
    10621 aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg
    10681 ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca
    10741 tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg
    10801 gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca
    10861 acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa
    10921 aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc
    10981 gtgacctggc gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac
    11041 agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg
    11101 catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca
    11161 gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg
    11221 aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg
    11281 cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg
    11341 acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga
    11401 tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg
    11461 ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg
    11521 ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc
    11581 tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc
    11641 tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg
    11701 ttgcggacct gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg
    11761 gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag
    11821 acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag
    11881 gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc
    11941 tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat
    12001 accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc
    12061 tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg
    12121 aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa
    12181 cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct
    12241 ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac
    12301 gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta
    12361 ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg
    12421 caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc
    12481 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
    12541 gggttttttt atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc
    12601 agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca
    12661 taaaaaaaca caacgctttc aacaaccttg cgtttttcat cttccgtagc ggcttggaat
    12721 aaatttacgt tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc
    12781 ccagtctttc cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg
    12841 tctgcgatcg tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc
    12901 tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg
    12961 aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa
    13021 ggcgcagatg gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc
    13081 agtacacggg cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg
    13141 cagccatagc aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc
    13201 aattagcgcg ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc
    13261 cagccgccag aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc
    13321 gtgatatgtc tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta
    13381 ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc
    13441 ggggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa
    13501 tagcagatgt tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc
    13561 atccagcgtt ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt
    13621 agcgcaaaga ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg
    13681 acgggtcaga cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga
    13741 taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc
    13801 agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg
    13861 attgctgaag gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga
    13921 gcaaaataac tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc
    13981 agacgaaatg ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact
    14041 aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca
    14101 gcatcgacaa ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt
    14161 tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact
    14221 tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc
    14281 tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg
    14341 gcgtcagggt gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg
    14401 gcggcgaaaa tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca
    14461 cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc
    14521 atcgccagtg tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa
    14581 gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt
    14641 aacatctcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat
    14701 atgttgcgtc cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc
    14761 ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat
    14821 cggttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc
    14881 ggcgcaacgc caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg
    14941 caggcgtctc tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca
    15001 ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat
    15061 ctggccacag gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac
    15121 cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat
    15181 taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa
    15241 ccgcatcttt caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg
    15301 gattcaactg tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag
    15361 tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tcagccagta
    15421 ccagcgtccc gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa
    15481 gaccaataat cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt
    15541 cgagcagaat ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca
    15601 atttccagca actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg
    15661 acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg
    15721 ccaatcgcca cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc
    15781 ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata
    15841 taagaggggc gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc
    15901 agccgcaggc ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc
    15961 aaatggacgc catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat
    16021 cgcgcgttat agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca
    16081 tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg
    16141 atccactgta cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa
    16201 tcaggctgat acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga
    16261 agttctccga catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta
    16321 cttcctggct gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga
    16381 ctttgccgga ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga
    16441 gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat
    16501 cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat
    16561 aagtgataag cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg
    16621 ttacgtaaca gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg
    16681 tgaagtggtc atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg
    16741 cttcgtggca gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat
    16801 ggcctgggcc ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc
    16861 ccagcgtatg caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc
    16921 gcagaccgtc gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc
    16981 ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg
    17041 aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca
    17101 ggacgcccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt
    17161 cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt
    17221 agatcggcac tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt
    17281 gaatatagcg accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata
    17341 ccagcttttc cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt
    17401 taacttttac caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt
    17461 tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat
    17521 tttccggcag atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac
    17581 ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga
    17641 tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg
    17701 ttaataagcc gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt
    17761 aatcggaact gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc
    17821 gtagttttgc cagcccttgc tgcacgttaa tggcgatctg cggatcacta tacgggccgg
    17881 aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc
    17941 caattagcgt cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc
    18001 cagtgatata aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat
    18061 gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca
    18121 aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg
    18181 cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc
    18241 ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca
    18301 aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac
    18361 gcgggcggcg tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag
    18421 cgcctcgccg atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc
    18481 ggtttccagg ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg
    18541 ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc
    18601 ataaatactg aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag
    18661 ggctttttcg tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta
    18721 gtaagccacg agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc
    18781 tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta
    18841 ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg
    18901 tagtgcaaga gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag
    18961 ccatgaacaa aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt
    19021 cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg cagggagaac ctgtttggtt
    19081 aatacaacag cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt
    19141 tgggctgttt caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa
    19201 atactgtggt tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg
    19261 cagccattgc cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg
    19321 tcgcgatgat tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt
    19381 acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat
    19441 ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt
    19501 tcctcagtct ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga
    19561 cccgaaagaa ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc
    19621 gcccggcacc gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta
    19681 tgagtgatga tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa
    19741 aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat
    19801 gatgcgccag gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga
    19861 ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg
    19921 tcgctacccg ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat
    19981 ggggttaggg ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg
    20041 caaagccgac gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat
    20101 gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc
    20161 cggcagcccg tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac
    20221 cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact
    20281 ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat
    20341 gattttcgac acctggggcg gcgtgcttat cgggcgcgat tatcaacagt tctcgctcta
    20401 ttacatgcat aaaattgttg atggtttact gcgtgaaaac gacggtcgct gcgtgccagt
    20461 cacgctgttt accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga
    20521 tgcgctgggc ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa
    20581 agtcgcgttg cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga
    20641 agaagaagta gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct
    20701 tggtcacggc attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt
    20761 gcatcgactg tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg
    20821 ccattttgag gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt
    20881 ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg
    20941 agcgattatg gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat
    21001 ctgcgaggat cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt
    21061 tgagcagggc ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga
    21121 gctggtggag tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct
    21181 ttccttccgc gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga
    21241 tttagtgttt gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca
    21301 ttttggctta ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa
    21361 attcgaaccg ctctccagcg aaccgggcgc gctggcccca ctgatggata aaggcgagca
    21421 gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca
    21481 tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct
    21541 gccggagcca acgcgctggg ctgacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta
    21601 tacagcaaat cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa
    21661 catgttacaa aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac
    21721 tttcatggct tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac
    21781 cggttttggt gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac
    21841 cgttaaagac gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc
    21901 ttcagccgac gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt
    21961 aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag
    22021 taagacctcc atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatgag
    22081 cgatgaagag cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat
    22141 attccgactt ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc
    22201 agacctgcgt gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa
    22261 aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt
    22321 ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc
    22381 tggcatttcc gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat
    22441 gaacaagact caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc
    22501 taaagctgct ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc
    22561 tgtacaactg gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg
    22621 caacccgcag accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc
    22681 tggcaaggca ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga
    22741 aggggtggtt tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct
    22801 gtttctcact gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc
    22861 tgaagaccag ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct
    22921 tgccgtgttt agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg
    22981 cgataacctc acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc
    23041 gcgttttgac gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca
    23101 gcagctttct aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat
    23161 tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga
    23221 acagaccgtg accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat
    23281 agcgcatatc gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga
    23341 agcgcccgaa ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc
    23401 caacgagaaa acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag
    23461 ccgccgtagc ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata
    23521 tctcgtttca cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc
    23581 gcgttaattt tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc
    23641 ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc
    23701 tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt
    23761 ccgccgtgag caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc
    23821 agggcaattt tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta
    23881 ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg
    23941 agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc
    24001 tttgtgttac agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa
    24061 gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta
    24121 aatggttaag cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga
    24181 cggtgattcg cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag
    24241 gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc
    24301 atcatgcgca gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt
    24361 ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa
    24421 aacagcttta ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc
    24481 ggcggatcga tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga
    24541 tgtttgctgc tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag
    24601 caattttcat cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa
    24661 acaccctgat tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct
    24721 tctggtatcg ccgctatatg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag
    24781 agaagctggt ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac
    24841 tttcctcgat taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag
    24901 cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg
    24961 agttgctgga actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc
    25021 tgattaacca ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac
    25081 gctttaccgc caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg
    25141 tcctgttgaa tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg
    25201 tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa
    25261 ttgcggcaga tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca
    25321 ccggattggg gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg
    25381 tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc
    25441 gtaaggaccc acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat
    25501 tagccactgc actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc
    25561 gaacagcggg cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg
    25621 cgatgtgcga atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa
    25681 cccggcaatt ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc
    25741 actgaaaact ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc
    25801 gacgctggaa aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac
    25861 tgccagccag ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat
    25921 cgccctcgtc gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa
    25981 agagctggtc gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac
    26041 gctcaactgt gcgacactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa
    26101 aggggcgttt actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg
    26161 cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg
    26221 tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt
    26281 ccggctgatt gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg
    26341 ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg
    26401 gcgggaagat atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg
    26461 caaggcggta aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc
    26521 gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga
    26581 atatatttcc gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca
    26641 aagtcaggat attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga
    26701 gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct
    26761 attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt
    26821 ccggcagaag caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc
    26881 ttctgccacg gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac
    26941 ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc
    27001 cagcggcagg ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc
    27061 catcaccacg ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc
    27121 ttcacaggcc gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg
    27181 ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc
    27241 gatcatcagg cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt
    27301 cacggttggc cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg
    27361 cgcgggggag taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg
    27421 tttgtgatcc tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa
    27481 gctcgcttct tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa
    27541 agcgttgccc gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac
    27601 gataacgcct ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc
    27661 acgcagatac accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat
    27721 cttatggcgg gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc
    27781 tgcggttggg ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac
    27841 cagcggcgct tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa
    27901 cagcgccggg atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt
    27961 gcctgcatta cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca
    28021 ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat
    28081 taatggcgga agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg
    28141 gcaatcactt cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca
    28201 gcggcggcgg catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa
    28261 cctttcactt ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg
    28321 cggctcatct ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca
    28381 ttggatttca cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg
    28441 gtcggctgac gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga
    28501 tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa gacccgaaac acgctcgccc
    28561 cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct
    28621 tcgctggcgg aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg
    28681 gtttccgcat ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg
    28741 gttttgtacg cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg
    28801 ttggcgtgct tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc
    28861 gcatcggtat cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta
    28921 gcaacggagg cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc
    28981 tcgccgtaac gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga
    29041 ccggcggctt ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca
    29101 atcatgctgt cgtaggcggc agtgtgttcg aaggctttga tggtgaggtc gaaacgggtt
    29161 gcaagcgtca gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg
    29221 ctcttcacca cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg
    29281 ccgccgatat cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc
    29341 tgggcgaacg gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc
    29401 ataatggcat cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg
    29461 gtcttcacgc gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc
    29521 ggcagacctt tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg
    29581 cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga
    29641 gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag
    29701 agcgttagct gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt
    29761 tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg
    29821 gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg
    29881 tggataactc tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca
    29941 gtcatttttc tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca
    30001 tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc
    30061 ctgaaattca gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg
    30121 cgctaaagcg cgtcgcaact gctctttaac aatttatcag acaatctgtg tgggcactcg
    30181 aagatacgga ttcttaacgt cgcaagacga gaaatgaata ccaagtctca agagt
//
LOCUS       DADKXM010000036        29584 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.036, whole genome shotgun sequence.
ACCESSION   DADKXM010000036 DADKXM010000000
VERSION     DADKXM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.036"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(344..419)
                     /locus_tag="JQR05_001600"
     tRNA            complement(344..419)
                     /locus_tag="JQR05_001600"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(384..386),aa:Phe,seq:gaa)
     gene            complement(526..1101)
                     /locus_tag="JQR05_001601"
     CDS             complement(526..1101)
                     /locus_tag="JQR05_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5323794.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(1138..2835)
                     /locus_tag="JQR05_001602"
     CDS             complement(1138..2835)
                     /locus_tag="JQR05_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY5323795.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(2811..3149)
                     /gene="cutA"
                     /locus_tag="JQR05_001603"
     CDS             complement(2811..3149)
                     /gene="cutA"
                     /locus_tag="JQR05_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY5323796.1"
                     /translation="MLDEKSSNTASVVVLCTAPYEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(3265..4566)
                     /locus_tag="JQR05_001604"
     CDS             complement(3265..4566)
                     /locus_tag="JQR05_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY5323797.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(4684..6120)
                     /gene="aspA"
                     /locus_tag="JQR05_001605"
     CDS             complement(4684..6120)
                     /gene="aspA"
                     /locus_tag="JQR05_001605"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY5323798.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6457..6933
                     /gene="fxsA"
                     /locus_tag="JQR05_001606"
     CDS             6457..6933
                     /gene="fxsA"
                     /locus_tag="JQR05_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY5323799.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(6949..8205)
                     /gene="yjeH"
                     /locus_tag="JQR05_001607"
     CDS             complement(6949..8205)
                     /gene="yjeH"
                     /locus_tag="JQR05_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY5323800.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            8481..8774
                     /locus_tag="JQR05_001608"
     CDS             8481..8774
                     /locus_tag="JQR05_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY5323801.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8818..10464
                     /gene="groL"
                     /locus_tag="JQR05_001609"
     CDS             8818..10464
                     /gene="groL"
                     /locus_tag="JQR05_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY5323802.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            10602..10955
                     /locus_tag="JQR05_001610"
     CDS             10602..10955
                     /locus_tag="JQR05_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY5323803.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11158..12027)
                     /locus_tag="JQR05_001611"
     CDS             complement(11158..12027)
                     /locus_tag="JQR05_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323804.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(12422..13450)
                     /gene="epmB"
                     /locus_tag="JQR05_001612"
     CDS             complement(12422..13450)
                     /gene="epmB"
                     /locus_tag="JQR05_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY5323805.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            13492..14058
                     /gene="efp"
                     /locus_tag="JQR05_001613"
     CDS             13492..14058
                     /gene="efp"
                     /locus_tag="JQR05_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY5323806.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            14110..14235
                     /gene="ecnA"
                     /locus_tag="JQR05_001614"
     CDS             14110..14235
                     /gene="ecnA"
                     /locus_tag="JQR05_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY5323807.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            14346..14492
                     /gene="ecnB"
                     /locus_tag="JQR05_001615"
     CDS             14346..14492
                     /gene="ecnB"
                     /locus_tag="JQR05_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY5323808.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            14668..14985
                     /locus_tag="JQR05_001616"
     CDS             14668..14985
                     /locus_tag="JQR05_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY5323809.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(14982..15515)
                     /locus_tag="JQR05_001617"
     CDS             complement(14982..15515)
                     /locus_tag="JQR05_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY5323810.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15604..16737)
                     /gene="blaEC"
                     /locus_tag="JQR05_001618"
     CDS             complement(15604..16737)
                     /gene="blaEC"
                     /locus_tag="JQR05_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY5323811.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(16800..17159)
                     /gene="frdD"
                     /locus_tag="JQR05_001619"
     CDS             complement(16800..17159)
                     /gene="frdD"
                     /locus_tag="JQR05_001619"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY5323812.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(17170..17565)
                     /gene="frdC"
                     /locus_tag="JQR05_001620"
     CDS             complement(17170..17565)
                     /gene="frdC"
                     /locus_tag="JQR05_001620"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY5323813.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(17576..18310)
                     /locus_tag="JQR05_001621"
     CDS             complement(17576..18310)
                     /locus_tag="JQR05_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY5323814.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(18303..20111)
                     /gene="frdA"
                     /locus_tag="JQR05_001622"
                     /pseudo
     CDS             complement(18303..20111)
                     /gene="frdA"
                     /locus_tag="JQR05_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
     gene            20436..21413
                     /gene="epmA"
                     /locus_tag="JQR05_001623"
     CDS             20436..21413
                     /gene="epmA"
                     /locus_tag="JQR05_001623"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY5323815.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            21632..23134
                     /gene="yjeM"
                     /locus_tag="JQR05_001624"
     CDS             21632..23134
                     /gene="yjeM"
                     /locus_tag="JQR05_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY5323816.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDMNFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(23285..26596)
                     /gene="mscM"
                     /locus_tag="JQR05_001625"
     CDS             complement(23285..26596)
                     /gene="mscM"
                     /locus_tag="JQR05_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY5323817.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMSQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(26624..27586)
                     /gene="psd"
                     /locus_tag="JQR05_001626"
     CDS             complement(26624..27586)
                     /gene="psd"
                     /locus_tag="JQR05_001626"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY5323818.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKD"
     gene            complement(27683..28735)
                     /gene="rsgA"
                     /locus_tag="JQR05_001627"
     CDS             complement(27683..28735)
                     /gene="rsgA"
                     /locus_tag="JQR05_001627"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY5323819.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            28830..29375
                     /gene="orn"
                     /locus_tag="JQR05_001628"
     CDS             28830..29375
                     /gene="orn"
                     /locus_tag="JQR05_001628"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY5323820.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         7127 a         7459 c         7716 g         7282 t
ORIGIN      
        1 tgttagtctt tgcatgagca agcggactga cacatttctc tttgttctca ttcagaaaat
       61 ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga tgcgttacgc tcctgatgac
      121 gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag ttagtgctat
      181 agctcaggaa caaattttcc caaattggga tatgctcgca aattgctggt gatgtgggga
      241 gaatctggtt gagttcggta gaattgattt ggaggcagaa cgcttaaatc gtggcgtcct
      301 gaaacgaaaa acggacctcc gtggaggtcc gttgatatga atttggtgcc cggactcgga
      361 atcgaaccaa ggacacgggg attttcaatc ccctgctcta ccgactgagc tatccgggca
      421 acggggcgca ttaaaccgta atctgcacat ctcgtcaacc taatttcagt aaaagcgatt
      481 caactgctta agattgcggc aaatcgcttc ttttctgtgt ttctgtcagg tcaatgcacc
      541 accctggcgg cagcgggcga agcgcaggat atcttccgcc agccgatgtg cagtgtcgac
      601 atccgcctgg ctacgattca ccagcattcg gcttaaacag ccttccagca ccagttccat
      661 ctgctttgct accatcgccg gatcgtcaac ttccagcgtg gttaacagtt cgtgggtgaa
      721 atcgtaggcc gcgctttttt gctgatcggc cagttgatga atagggtggc caggatcggg
      781 ataaaacgta caggcagcga taaatagaca gcccggatag cggttgtttt taacgcactc
      841 cgataacgcc tgataacgtg ccagcagctt ttgttcggcg gtttgcgttt cgtccagcat
      901 cagctgacga cgccagacat ctatctgttg gctaagataa cgcagcgcat cgtagaggat
      961 tgcctctttg tctggccaga agcggcgtag ctcgtccagt ggataatcca cacgttcagc
     1021 aaccatctcc agcgtggtgt tggcaatccc ttgtaattct aataatttca gggcttctcc
     1081 cagtacatct tcacgttgca cgctattttc ctccgtcttt cccactgcaa gtgtcgttca
     1141 cggttggcga tcgcgcaaat gtgcgctgaa ggtttcagca tccataaagc ccgtgacgcg
     1201 tgcttgtgga tgctcctggc cttgtccgtc aaaaaagaga attgtcggta ggccaaggac
     1261 attaagatgc tttaacagcg ccacatcttg tgcgtcgttg gccgtgacgt tggcctgaag
     1321 taagaccgtg tctgctaacg ctttttgcac ctgcgggtcg ctgaaggtgt atttctcaaa
     1381 ctctttacag gcgacgcacc agtcggcata aagatctaac atcaccggtt tgcctttggc
     1441 ttcaacgagc gcctgattta actcatctac cgttttgatt tgtgtaaagt tgagatgcgt
     1501 ctgagtttgc gcggtatgcg tcgcaccaaa tgcccaatcc tgaagtgggc gcacgctaac
     1561 caatgccgct gccagcagga ttatttgcac cacgcgcatc cagccgcgtt tagcctgtag
     1621 gctggtgata aaggcccagc caaagaatgc gacacccagc gccgaccaca agcgtaatcc
     1681 ccatacatca ccaatcactc gctccagcag gaagaccggc agtgcgagga tcacaaaacc
     1741 aaacgcggtt ttgacttgtt ccatccacgg gccgcttttc ggcagcaagc ggttaccaaa
     1801 gacggtaatt agcatcagcg gcaggcccat gcccaacgca tagagataaa gcgtgccgcc
     1861 gcccagccac atgttcccgc tttgggcgat atacagcaga atcgcgctaa gcggtgcggt
     1921 ggtgcatggt gaacagatca gtccggcaat cgcccccata acaaacacac cgccaggtga
     1981 gccgccctgt tggcgattgc tcatcaacgt gagacgtgtt tgcagcgaag aggggagttg
     2041 tagagtaaac aagccaaaca ttgacatcgc cagcaaggta aagacgatgg cgaggccaat
     2101 gagcacgtat gggtgctgta gcgccgcctg gaactgtaac cctgcggcgg caactaccag
     2161 acccagcgcc gtgtaagtca gtgccatccc ctgcacataa ataaaggtca gcaacaatgc
     2221 tctggcagtg gagagccgct gtttaccacc cagcacgatg ccagaaatca gtgggtacat
     2281 tggcagcacg catggcgtaa aggcgatacc aataccgatc aacaacgccc agagcgcgga
     2341 aaagggcaat tgcgcggtgt gctgctcttg ctgcgaaaca gacacaggct gtgacgctgc
     2401 gttgttggcg accacttcgc ttaacggaac ggttttggtt tctggcggat aacagaaacc
     2461 ggcatcagca cagccctggt aggtgacagt taacgtcgct cccgcactcg cctggttgat
     2521 ggttacggga agcgtcagcc gatcgcggta aatctcgctt ttgccgtaaa actcatcttc
     2581 atgccagacg ccttgcggca gctgcacgtc ggcaattttc gcgtgttccg gcgtaatgcg
     2641 gatctgttta cggtagaggt agtaaccgtc tttgatctgc caggtcagat taaggtcatg
     2701 ttggttttgc tgaaaatcaa aagcaaaggc ttgatccgcg gggacaaatt gtgaacgtcc
     2761 cggcgcgtcg aataatccgg caaaaacgga agtgctgcaa agtagcagaa tcagcgtaaa
     2821 gatgcgttga gccatgagag gtaatctgtg tctccgtgtg taacaggtaa aaccagaagt
     2881 tccggggttt gatatggatg atgagacttc aggcattcca gcagtgcctg ctggtgagat
     2941 acggtagttt ttaaaatcat ctgcacttcg tattcttgct ccagcttacc ttcccagtaa
     3001 tagagagagg tagcgccggg gatcaaggtc gcgcaggccg ccagtttttc cgccagcact
     3061 ttggcggcta aatcctgggc tgtcgcttca tacggtgcgg tacatagcac cacgacagac
     3121 gcggtattcg aacttttttc atcaagcata aacacctcgc aagaacagat ggaatcgcaa
     3181 gagagaggtc actatacaac ggacggggga aggatgttag tcggcgggaa agaaagcggg
     3241 gcgtgaacgc cccgcgattg atcattacag catgaagcta cccagcacga agccgaagca
     3301 aacggccagg gcaacaccca gagtacccgg gatgaagaac ggatggttga agacgaattt
     3361 accgatacgg gtagtacccg tgtcatccat ctgtaccgca gcaaccagcg tcgggtaggt
     3421 cggcagaatg aacagaccag acaccgcagc gaaagaagca acagcggtca gcggtgaaac
     3481 gttcagtgcc agagccatcg gcatcagtgc ttttgcggtt gcagcctgag agtacagcag
     3541 agcagaagca aagaagaaga tgacggccag cagccacgga tgaccctgaa tcacttcacc
     3601 agcggtatct ttgatccagt cgatgttgtt ggaaacgaaa gtatcgccca gccacgcaac
     3661 acccaggata caaatacagg cgctcatacc tgctttgaag gtgctggagt tgaggatgtt
     3721 gtcggtatcg actttacaga taacggtggt cagagttgca acgctgagca tgatgatcag
     3781 gattgcgttg gtggtgttca tcagcggttt ttcaaccaga cccatgcttg ggctgttgat
     3841 gattgcatag ataaccacgc caactacgcc cagcaggaac agccagacgg acgtttttgc
     3901 accggatttg atttcaatct gcttttcacc gcgcagttca accaagccct cttccagacg
     3961 cttgcgataa atcggatcgt cagagagttt ggagttgaac agcatagtga ccaggaagga
     4021 catcaccaga accgccagca gggtggacgg gatgaccacg gagagcagat ggaggtagct
     4081 gatgccatga ccttccatca cggaagacat gtaaaccact gccgctgaga ttggcgatgc
     4141 ggtgatcgca atctgcgcgg ataccactgc agtagagagc ggacggcaag gtttaacgcc
     4201 ttgttccttc gcaacttcag cgataactgg cagtgtcgcc agagagatgt tgccagtacc
     4261 agcaaagata gtcaggaaat aggtcacgat cggtgcgagg atcgtgatgt atttcgggtt
     4321 acggcgcagc agcttttctg tctgatgaac cagatagtcc agaccgccag caacctgcat
     4381 ggcagaaata gcggcgataa ccgccatgat aatggaaatg acatcgaacg ggatgttacc
     4441 gggtttaacg ccaatagcgg caagaaccag cacccccaat ccgcctgcaa aaccaatacc
     4501 tattcccccc aatctggcgc ccaagaagat cgccagcaaa actatgatga gttctacaac
     4561 tagcatatta gccttccttg ttttttaaca agttgatatt agattgttat ttttaagtta
     4621 ctgctcacaa gaaaaaaggc acgtcatctg acgtgccttt tttatttgta ctaccctgta
     4681 cgattactgt tcgctttcat cagtatagcg ttttgctttg taagccgggt gcatcagatt
     4741 ctgtacggag aaaatatcgt caagttccgc ttcagtcaac agaccgcgtt ccagaacgac
     4801 ttcacgtaca ctcttaccgg tttcggcaca gattttaccc acgatgtcac cgttgtggtg
     4861 accgatgaac gggttcaggt aagtaacgat accgatagag ttgtaaacgt aaccttcgca
     4921 cacttctttg ttagcagtga tgccgttaat gcatttttcc agcaggttgt agcaagcgtt
     4981 ggtcagaatg tgaacggatt cgaacatggc ctggccaatg accggttcca taacgttcaa
     5041 ctgcagctga cctgcttctg ctgccatggt aacagtggtg tcgttaccga tgactttgaa
     5101 gcatacctgg ttaaccactt ccggaacaac cgggtttact ttagctggca tgatggaaga
     5161 gcccgcctgc agttccggca ggttgatctc gttcaggccg gcacgtggac cagaagagag
     5221 caagcgcagg tcgttacaga ttttggacat cttcacagcc aggcgtttca gcgcgccgtg
     5281 aaccataaca taagcgccgc agtcagaggt cgcttcgatc aggtcttcag ccggtacgca
     5341 tgggaagcca gtgacttcag ccagtttttt cactgccagc ggagagtact ctttcggcgt
     5401 gttcagacca gtaccgattg ctgttgcgcc aaggttaact tccagcagca gttcagcggt
     5461 acgttggatg tttttcactt cttctttcag caggatgctg aaagcgcgga attcctgacc
     5521 gagggtcatc ggtactgcgt cctgcagctg ggtacgaccc attttcagga tgtcctggaa
     5581 ttcgacagct ttacgttcaa agccttcacg cagttggtta atcgcatcta ccagcttaat
     5641 cagggaagag taaactgcga tacggaaacc ggtcgggtag gcgtcgttag tggactgaca
     5701 tttgttaaca tggtcgttcg ggttcaggta ctgatattca cctttctggt gacccatcag
     5761 ttccagaccg atattggcca gcacttcgtt ggtgttcatg tttacggaag tacctgcgcc
     5821 gccctggtag acgtctaccg ggaactgatc catgcatttt ccgttgttca ggacttcatc
     5881 acatgcggca atgatggcat tcgctacact tttaggaatg gtttgcagct ctttgtttgc
     5941 catagctgcg gcttttttaa ccattaccat accgcgaaca aattcaggaa tatcactgat
     6001 tttgttgttg ctgatataga agttttcaat cgctctcaga gtgtgaacac catagtaggc
     6061 atcagctgga acttccctgg tacccaacag atcttcttcg atacgaatgt tgtttgacat
     6121 gtgaaccttc tttttcaagc tgccaatgat ttgctttaaa cacacagaat atatgtggtt
     6181 tcgaatgttt ttcgaccgac gattatcccc tgcatcgacc gaatacccga gatcatatgc
     6241 tgcttgagga tttctaccgt aatctggatc actttaagtg tcggttttta ccccttaatt
     6301 attaatttgt gaaatagatc accgctttgg gattactacc aaaaatagtt gcgcaaacat
     6361 cttgaaattt tgctaatgac cacaatataa gctgaacgcg attcgcaacc cattcaggta
     6421 gccgggttta accggctgct attacaggag aaacctttgc gctggttacc ttttattgcc
     6481 attttccttt atgtctatat tgagatttca atctttattc aggttgccca tgtattgggg
     6541 gtattgctga ccctcgtgct ggttatattc acgtcagtta tcggtatgtc actggtacgt
     6601 aaccagggct ttaagaattt cgtgctgatg cagcaaaaaa tggcggcggg tgaaaatcct
     6661 gcggcggaga tgattaaaag tgtttcgctg attattgccg gtttgctgct tttattaccg
     6721 ggctttttca ccgacttcct cggtcttctg cttttattgc cgccggtgca aaagcatctg
     6781 acagtgaagt tgatgccaca tttgcgcttt tctcgcatgc caggcggcgg ttttagcgcc
     6841 gggaccggtg gcggtaatac ttttgatggt gagtatcagc gaaaggatga tgaccgcgac
     6901 cgccttgatc ataaagacga tcgccaggat taatgtcgaa acgccggatt atgtggttat
     6961 gccattttcc ggcgtttttc gttttggcag aaacagccat aaccccacca gcatgatcag
     7021 cgcatagaga cttttccagc cgaccattgc cagtaacaga acgcataaca gcccgccaac
     7081 caccgccagt agtcgataac gaccttgcaa taatttacag cctgccagca tgcataacag
     7141 ataaatcata ataaagatgc cattggcata aataataaga gcgtccagat tgatctctaa
     7201 ggcatgaatt accaacgtgc tcaccacgca gcagccaagc accgcattga gggcattatt
     7261 cgggatatgg cgagaagaga ggcgtgctag gtagtggtca ggattatgtt gcgcctgcga
     7321 ccagaccagg cgggcgaagc tctgtatata aatgttaaga ctggcaaagc aggccagata
     7381 gccaattacg caggcaatcc ataacgctcc tacaccgaac aactgcacta caatttttgg
     7441 aagcgatgct gccgccgcca tttgttcacc ataggcgtcg aagtgtaaga cgactaccgt
     7501 acagccccag tagactaatc ctgccagcag cagaccaatc atcaaagcac gaggaaaatc
     7561 acgctctgga tttttaaatt ccgaggcaag atgggcaaat gcttccagac cgacaaaaca
     7621 ccagaacatc actgataacg cagcaaataa cccggtaagt tcgatatttc ctggcgcggg
     7681 gaaggggata ctcgcaggtt tgatatcgcc cgcccaccag atagcgacaa tcagtgcgac
     7741 gataagtccg gcaataacgg tttgtagatt agcactggaa ctggcaccga gagtaccgat
     7801 ataccacacc aacgccagcg taccgagttc tgccaacaac agttgccagc tatgccagcc
     7861 aaacattgcc tggccgaatc cggcggcaat ttgtagtgcg gcaggcaaac ccacgggaat
     7921 gaccgataaa aacagccagc cggtgactcg ctcaagccgc gaaccaaacg ccataccgac
     7981 gaagtgcgcg acgccgcctg cgctgggata gtggcgaccc agaatcgcaa acacaatcgc
     8041 aatcgggaac actaagataa tcaaaacggg ccacgcccac aggctgttat tgcctgctac
     8101 cagtgcagct aacgcaggaa cggcaaacac gccagtgcct aataatgacg tcgatagcag
     8161 gccaatgccc tgggccagcc ccagttcttg tttgagtcca ctcatgggtt gatatccgat
     8221 tgcgcccaaa ttttgggcaa ctgcgtagat tttcgatggt agcacaatca gattcgctta
     8281 tgacggcgat gaagaaattg tgatgaaatg tgaggtgaat cagggttttc acccgatttt
     8341 gtgctgatca gaattttttt tctttttccc ccttgaaggg gcgaagcctc atccccattt
     8401 ctctggtcac cagccgggaa accacgtaag ctccggcgtc acccataaca gatacggact
     8461 ttctcaaagg agagttatca atgaatattc gtccattgca tgatcgcgtg atcgtcaagc
     8521 gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct gaccggctct gcagcggcta
     8581 aatccacccg cggcgaagtg ctggctgtcg gcaatggccg tatccttgaa aatggcgaag
     8641 tgaagccgct ggacgtgaaa gttggcgaca tcgttatttt caacgatggc tacggtgtga
     8701 aatccgagaa gatcgacaat gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa
     8761 ttgttgaagc gtaatccgcg cacgacactg aacatacgaa tttaaggaat aaagataatg
     8821 gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga aaatgctgcg cggcgtaaac
     8881 gtactggcag atgcagtgaa agttaccctc ggtccaaaag gccgtaacgt agttctggat
     8941 aaatctttcg gtgcaccgac catcaccaaa gatggtgttt ccgttgctcg tgaaatcgaa
     9001 ctggaagaca agttcgaaaa tatgggtgcg cagatggtga aagaagttgc ctccaaagcg
     9061 aacgacgctg caggcgacgg taccaccact gcaaccgtac tggctcaggc tatcatcact
     9121 gaaggtctga aagctgttgc tgcgggcatg aacccgatgg acctgaaacg tggtatcgac
     9181 aaagcggtta ccgctgcagt tgaagaactg aaagcgctgt ccgtaccgtg ctctgattct
     9241 aaagcgattg ctcaggttgg taccatctcc gctaactccg acgaaaccgt aggtaaactg
     9301 atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta tcaccgttga agacggtacc
     9361 ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt tcgaccgtgg ctacctgtct
     9421 ccttacttca tcaacaagcc ggaaactggc gcagtagaac tggaaagccc gttcatcctg
     9481 ctggctgaca agaaaatctc caacatccgc gaaatgctgc cggttctgga agctgttgcc
     9541 aaagcaggca aaccgctgct gatcatcgct gaagatgttg aaggcgaagc gctggcaact
     9601 ctggttgtta acaccatgcg tggcatcgtg aaagttgctg cggttaaagc tccgggcttc
     9661 ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc tgactggcgg taccgtaatc
     9721 tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg aagacctggg tcaggctaaa
     9781 cgtgttgtga tcaacaaaga caccaccact atcatcgatg gcgtgggtga agaagctgca
     9841 atccagggcc gtgttgctca gatccgtcag cagattgaag aagcaacttc tgattacgac
     9901 cgtgaaaaac tgcaggagcg cgtagcgaaa ctggcaggcg gcgttgcagt tatcaaagta
     9961 ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac gcgttgaaga tgccctgcac
    10021 gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg gtggtgttgc gctgatccgc
    10081 gtagcgtcta aactggctga cctgcgtggt cagaacgaag accagaacgt gggtatcaaa
    10141 gttgcactgc gtgcaatgga agctccgctg cgtcagatcg tcctgaactg cggcgaagaa
    10201 ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca actacggtta caacgcagca
    10261 accgaagaat acggcaacat gatcgacatg ggtatcctgg acccaaccaa agtaacccgt
    10321 tctgctctgc agtacgcagc ttctgtggct ggcctgatga tcaccaccga atgcatggtt
    10381 accgacctgc cgaaaaacga tgcagctgac ttaggcgctg ctggcggtat gggcggcatg
    10441 ggtggcatgg gcggcatgat gtaattgccc tgcacttcgc agaaataaac aaacccccgg
    10501 gcagaaatgt ctgggggttt ttcttttggt catctttcta gtataagatt cagacacgga
    10561 cgacgcgagt ggcgtccagc tcattgatta tggggaataa catgcacgta aaatacttag
    10621 cagggattgt cggtgccgcg ctactgatgg cgggttgtag ctccagcaac gaattgagtg
    10681 ctgccggtca gagtgtacgc attgtggacg agcagccagg cgcagagtgc cagctgattg
    10741 gtactgcgac aggtaagcaa agcaactggc tttccgggca acacggagaa gagggtggtt
    10801 ctatgcgcgg cgcagcaaac gatctgcgca accaggcggc tgcaatgggc ggtaacgtga
    10861 tttatggcat cagcagcccg tcgcagggaa tgttgtccag ttttgtcccg acggatagcc
    10921 agattatcgg tcaggtttat aagtgcccga actgatgtga tccagaggct gggtgacaaa
    10981 cgtattattg cctgatgcgc ttcgcttatc aggcctacag caggcattgc aactgattga
    11041 atgcttgaga atttgtaggc cggataaggc gtttacgccg catccggcat gaccaacgat
    11101 cacgttatca gtcatttcag gctggatggc tccagcctcg cattagcaaa gatgagatta
    11161 tttcgcctgt ggtgcagttt tgctggtgga ttgtacacgt aagtgatgca gcgcgcgtaa
    11221 gcattgatcc tgggttagcg gttgggcgtt attcgccgct acggtgcgaa catagacctg
    11281 acatggcaca cccgcgagtt ttttcagtcg tgggctgaaa tcaagcattc ggcgagtgat
    11341 cgcctctatt tctttacccg gcgttgcttc acgcgttttc accactgcgc cgctaaattg
    11401 ttctttgcca ctttcatctt taaagcggta aagcaccgcc agataatggt cgaacaagtt
    11461 atgtttccat tccggttggg aataggtgtc atattccaga ttgccattgt cctggcagtc
    11521 aacgtcgtat aacggcagga aatctaacag tgaggtaata cgtaatgcca gttcacgcat
    11581 ttccgcatta ttaattgcat gaatgattgc tcgcgccagc atttcaatct gcaactcatt
    11641 gacgactaac tcacatttgc taccgtcatg tagcgttaat acgaaggtaa gattttcgcc
    11701 ttgattatcg gtaagttcca gcgtgttaac ccgacgatta atatcggcat ttttaagctc
    11761 atcaaccaga atttctggga tgttttctgc cattttctta atgactttat cgcgagcttg
    11821 ctcatactga aggcgtgcag cagcatccag tttatgcttt tgatgcagac gactttccag
    11881 cgcaatcagc aggtcgcgca gttccatgac agacataaag aacaacgatt ctttattgcg
    11941 aggttcttta attttcagcg ccagtgcaat gaaattatta ctcttgcgaa taacaccggt
    12001 attaacaccc ttgatgctta tggccatgta ggttctccct aaccatttct caattaaata
    12061 attaatttta atttataagc cagataaatg ggcttggtag taatagttgt taaaataaca
    12121 taaatagccg tgctcactct atataaacag aaaagaaagg ttaattattg gtgttagcta
    12181 tataaaaagt aacaacagca atgcatatga ataattaaag gttatgcaac gtgcaaagat
    12241 taaatttctc agtttaatga caggttgctg tacttgcgtt tgttgctctt gccggatgcg
    12301 gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc attcaatttc aatgcacgat
    12361 gtaggcctga taagcgtagc gcatcaggca attttaatgt ttaacttccc tgtttaatca
    12421 gttactgctg gcgtagctgg agatccagcg gcgttttgct gggttcgccg ccaatttctc
    12481 gcgccagttt cggcaccaga tatcccgaca ccaatgtcag caactcacgc ataatctgcc
    12541 gtgcttcgtc atcactcacc ataaaatgcg ccgcgccctg tactttatct agcacatgca
    12601 ggtaatacgg catcacgccg gcatcgaaca acgcattact caggtttgcc aacgtttgtg
    12661 cgttatcgtt cacaccacgt aacagaacgc tctggttaag cagggtgaca ccgacacggc
    12721 gcaatttagc catcgcctga cggaacgttt catctacctc attggcatga ttgatgtgat
    12781 tcaccagcaa gatttgcagc gtagaacggg caaagcgttc aaccagcgcc tcggtgatac
    12841 gtgccgggat cacaatcggc agacggctgt gaatccgcag acgtttgata tgcgggatgg
    12901 cttccagttg tgtgagcaac cagtccagct cgtgatcttt cgccatcagc ggatcgccgc
    12961 cggagaaaat catctcgtcc agttccggat gcgcagcaac atactcaagt gcagtctgcc
    13021 agttacgctt gttgccctga ttttcggcat aggggaagtg gcgacggaag caatagcggc
    13081 aatttaccgc gcagccgcct ttgaccagca aaagcgcccg gttgtggtat ttatgcaaca
    13141 aaccaggcac tacgctgtgc tgttcttcca gtgggtcggt ggagaatccg gacgcgacga
    13201 caaactcatc ttgcgaggta agtacctgac gcaaaagagg atcgtccgga ttgcctttct
    13261 ccatgcgatc gataaatgag cggggcacac gcagggcaaa cagctttttg gcgctgcgtc
    13321 cggccaacag tttttcgtcc gcgtctatat tcaaaagacg cagaagttca tcaggatcgg
    13381 tcacaacatc ggcaagttgc gttaaccaat cttctctgga tggggtattt agggttacaa
    13441 tatgcgccat tttgtggctt agctaccaat taacaaattt cagagggcct tatggcaacg
    13501 tactatagca acgattttcg tgctggtctt aaaatcatgt tagacggcga accttacgcg
    13561 gttgaagcga gtgaattcgt aaaaccgggt aaaggccagg catttgctcg cgttaaactg
    13621 cgtcgtctgc tgaccggtac tcgcgtagaa aaaaccttca aatctactga ttccgctgaa
    13681 ggcgctgatg ttgtcgatat gaacctgact tacctgtaca acgacggtga gttctggcac
    13741 ttcatgaaca acgaaacttt cgagcagctg tctgctgatg caaaagcaat tggcgacaac
    13801 gctaaatggc tgctggatca ggcagagtgt atcgtaactc tgtggaatgg tcagccgatc
    13861 tccgttactc cgccgaactt cgttgaactg gaaatcgttg ataccgatcc gggcctgaaa
    13921 ggtgataccg caggtactgg cggcaaaccg gctaccctgt ctactggcgc tgtggttaaa
    13981 gttccgctgt tcgtacaaat cggcgaagtc atcaaagtgg atacccgctc tggtgaatac
    14041 gtctctcgcg tgaagtaatg cggttgtggt gcggcctgca ggctgcacca tcacttattc
    14101 aggtcagaga tgatgaaacg ccttatcgtt cttgttttgc ttgccagcac gctgctcacg
    14161 ggctgtaaca ccgcttgcgg tttcggcgaa gacatcaaac atctcggcaa ctccatctct
    14221 cgcgctgcca gctaattttt cttctcttcc gaaaaatcat cagatttcca tcatttttgg
    14281 cgatgttgtc tattattaat ttgctatagg caaacataaa taacattacc taaaaggaag
    14341 acgttatggt gaagaagaca attgcagcga tcttttctgt tctggtgctt tcaacagtat
    14401 taactgcctg caacaccacg cgtggcgttg gtgaagacat ttctgatggc ggtaacgcga
    14461 tttctggcgc agcaacgaaa gcgcagcaat aagcaataac ggtacgacag cagtgtcgtg
    14521 ccgtttgttt tttctgcgat cgtcataaag gtaatagttg aaattcccct gccacctggc
    14581 aaaatatccg ttcaaccatc agctttgcag gacgacctgc aaacgcctct tttcaccggg
    14641 gacggcccca aatttccgga gcctgatatg tcctggatta tcttagttat tgctggtctg
    14701 ctggaagtgg tatgggccgt tggcctgaaa tatacccacg gctttagccg tttgacgccg
    14761 agtgttatta ccgtgacggc gatgatagtc agtatggcgc tacttgcctg ggcgatgaaa
    14821 tcgttaccgg tagggacggc ttatgccgtg tggacgggta ttggcgcagt cggcgcggcc
    14881 atcaccggca ttgtgctgct cggtgagtcc gctaacccga tgcgtctggc tagtctggcg
    14941 ttaatcgtat tggggattat tggtctgaaa ctcagcactc actaactacc gggctgctgt
    15001 acccaaataa acttactgac atcaaaccct tcccgggtcg cgactgccag catctcctgt
    15061 ttcacttcgt cagaaatggt tggcgtgcgg gagagtatcc acaggtagtc gcggtccggg
    15121 ccgcaaacca gcgcatggcg gtattcccga tcgagtgcaa taacgttata accgccatag
    15181 aaaggaccaa agaaggacac tttcagcgca gcgcgagttg gtgcgccggt aaagtacgct
    15241 ttcccttcac tctgctgcca cattcctctg tcagggttat agcctttatt aatgacattc
    15301 aggccgccgt catcacgcag gctgtatgtt gcggtgactt tttccagtcc acgttcaaaa
    15361 cggtgatcaa aacgggcaat ctcataccag gtaccgagat aacgtttggc gtcgaaatta
    15421 ttcactacgg tcacgccacg cggcggcgta ggagaactac aggcaacgac cagaaatgca
    15481 gctgtcgctg cggcaacgag agggagcagg cgcataaatg tttctttact ggtttttttc
    15541 taagtgtaga tgacagcaag gaaaagcgca gaaaaggtcc gaaaattcgg acccgatgaa
    15601 attttactgt agagcgttga gaatctgaca ggcggcggcg actctcgctg gattgggata
    15661 gtttttgtta gccagcatca cgatacccag ctctttttct ggaataaacg cgacatagct
    15721 accaaatccg ccggtcgccc ccgttttatg tacccatgat gcgcgtactg caggagttgg
    15781 gggcttaatc gcttttaccg ggcgtgctgc cagtgcaatt ttattgtcac tgccgttaat
    15841 gatgctgtca ggattgaccg gccagtccag catttcccag cccaggccct gatacatatc
    15901 gccggtttgc cagtagcgag attgtgccag ttgtatccct tgttgaagcg ttttctcatt
    15961 gatatcaagg ggggttaaat tgctttgcac ccagcgggcc atatcttcaa tggtcgactt
    16021 cacaccataa gcttcagcat ctaacgcccc tggcgaaaca tgcactgcct taccttcgcg
    16081 atatccccag gcgtaattct tttcttctgg gggaggtaca ttaatccacg tatggttgag
    16141 tttgagtggc tggaagacac gagtttgcat cgcctgctca aaactcaaac cagacggctt
    16201 cacagccagt gcgccgaaca aaccgatact ggagttggca tacagacgtt gtgttcctgg
    16261 agcccatgca ggctgccagt tttgatagaa gcgcagcaag tcgcttgagg atttcacttc
    16321 atccggcacc tgcaatggca ggccgccagc ggtgtaggtc gcaagatgta atagtgtgat
    16381 cccattccac tgtttagcgg taagttcagg ccagtatttt gttgcgggat cgcttaactt
    16441 gatttcccct cgagcaatag cgtcgccacc aagcacgccc gtaaatgttt tgctgaccga
    16501 acctaactca aacaacgttt gctgtgtgac gggctgcttt ttggcgatgt ccgcatagcc
    16561 ccaggtaaag taataaggtt taccctgata aattaccgcc accgccatac cggggatctt
    16621 ttgttgctct ataagcgggg taattgtgcg atgcacaata tcgttgattt gttgaggggc
    16681 agcaaatgtg gagcaagagg cggtaattaa taaggcgcag agcgtcgttt tgaacataag
    16741 gtctggtttc catacaaaac ggcccgccat aggcgggccg gatttacatt ggcgatgcgt
    16801 tagattgtaa cgacaccaat cagcgtgaca actgtcagga tagcagccag accgtagaaa
    16861 acccatttgc ccgcaggcac gtggattttc agatcgtgca tcgcgtggtg catacggtgt
    16921 aaaccacacc acagcggcag aacgatcatc aggaacagga atacgcgacc aatgaagctc
    16981 tgcgcgaacg ccagaacgcg ctcgtagctc agcgcatcac ccggaaacag ccccagtggc
    17041 agcagaatac ccaccagcag gatcatcacc ggcgcaataa ttgcgctcca cataccaccg
    17101 gccccgaaga ggccccagaa taccggttcg tcagaacgct ttggatttgg attaatcatc
    17161 tcaggctcct taccagtaca gggcaacaaa caggattacg atggtggcaa ccacagttac
    17221 cgcccagaga cttttgataa ttggctctgg tcccattttt tcgtctttta caatgatatt
    17281 ggccgctttc ggtgccagtt caaaccaggt tttggtgtgc agcagagctg ccgtcagagt
    17341 gatcaggtta atgatcacga taaccgggtt ttgtaaaaag tcgacgaatc ccgcccaggc
    17401 ttccgggcca tttttcaggg caaacagccc gaaaatcagt tcaatgctga accacacagc
    17461 cggaaccgcc gtgccttcgc gcagcatgta aaagcgataa aacggcaatt ttttccacca
    17521 ggtggacgtc attggccgta catacggttt acgtttagtc gtcatgttgc actccttagc
    17581 gtggtttcag ggtcgcgata agaaagtctt tcgaactttc tactttgccc tgctgaatgg
    17641 ccgcagccgg atcgacgtgt ttcgggcaga cttcggagca atagcccacg aaagtacagc
    17701 tccatacacc gttctggctg ttcaactgcg ccatacgctc cttcttaccg tggtcgcggc
    17761 tatcttcgtt ataacgatgc gccagcgtaa tggcagccgg accgatgaac tctgggttca
    17821 ggccaaactg cgggcacgcg gcgtagcaca gaccacagtt gatgcaaccg gagaactggt
    17881 gatactttgc catctgcgcc ggggtctgga tgttagtacc ctgatccgcg gtgcgggagt
    17941 tgccgatgat gtacggtttg atcgcttcca gactttcgat gaagtgggtc atatcgacca
    18001 ccagatcgcg ttcaatcggg aagttagcta acgcttcaac cttcataccg tcggtgtaat
    18061 cacgcaggaa ggttttacat gccagttttg gcacgttgtt aaccatcatg ccgcaggaac
    18121 cacaaattgc catacggcag gaccagcggt aactcaggtc cggtgccagg ttgtctttga
    18181 tgtagcccag cgcatccagt aatgaggtag ttgcgtcata aggcacttca tagaatgcgc
    18241 tatgcggtgc ggtatcgact tccgggttat agcgcaccac ctcaattttc aggtttttca
    18301 tctcagccat tcgccttctc cttcttattg gctgcttccg ccttatcggc tgcatccgct
    18361 tcgccaccgt aaacgcgttt agctggcggc agcgtagtaa tcttcacgtc gctgtactcc
    18421 aggcgagtcg tgccatcagc atcgcggaag gcgagggtgt gtttgagaaa gttgacgtcg
    18481 tcacgctcgg tgcaaccttc gtccagacgc tggtgtgcgc cgcgggactc tttacgtgcc
    18541 attgcggagt gcgccataca ttcagcaacg ttcagaccgt ggcccagttc aatggtgtag
    18601 agcaggtcgg tgttgaacac gctggaggtg tcggtgatgc gcacgcgctt gaagcgttcc
    18661 tgcagctctg ccagcttgtc gatggttttc tgcatcagtt ccggcgtacg gtagataccg
    18721 caaccttctt ccattgccag gcccatttcg tcgcggatct tcgcccagtt ttcgccgcca
    18781 tcctggttaa ccagatcttt cagacgttgt tcaacgccag ctgcctgcgc ttcaattgcc
    18841 gcttcgttgc cattaccggc agttgctgca cgctctgtcg cttgttcacc ggccagacgg
    18901 ccgaagacca ccagttccgc cagggagttg gagcccagac ggtttgcacc gtgcagacca
    18961 acagaggaac attcacccac ggcgaacaga cctttaatgc gggtttcaca gttctgatcg
    19021 gtttcgatac cgcccatggt gtagtgtgca gtcggacgta ccggaatcgg ttctttaacc
    19081 ggatcgacgc caacgtacgc tttcgccagt tcgcagatga acggcagacg ttcatgcagt
    19141 tttttctcgc cgaggtgacg caggtcgaga taaaccacat caccacgcgg cgtggagatg
    19201 gtgttgcctt tacgccattc gtgccagaag gcctgagaaa ctttgtcgcg tggacccagt
    19261 tccatatatt tgtttttcgg ctcgcccagc ggagtttccg ggcccatgcc gtaatcttgc
    19321 agataacggt agccattttt gttgaccaga ataccgcctt caccacggca gccttcggtc
    19381 atcaggatac cggaacctgg cagaccggtt gggtgatact gaacgaattc catgtcacgc
    19441 agcggaacgc cgtggcttag cgccataccc ataccgtcac cggtaacgat gccgccgttg
    19501 gtgttgtaac gataaacgcg acccgcaccg ccggtagcca taacgaccgc gttagcacgg
    19561 atctgcacca acgtgccttc catcatgttc attgctacca ggccgcgaac atgaccatca
    19621 tcaaccagaa tatccagcac gaaatgttcg tcaaaacgct ggatctgcgg gaattgcaga
    19681 gaggtctgga acagcgtgtg cagcatatgg aagccggtct tatcggcggc gaactaggtg
    19741 cgctcgattt tcatgccgcc gaagcgacgt acgttgacgc taccatccgg gcgacggctc
    19801 catgggcatc cccacagttc cagttgggtc atttcggttg ggcagtggtg gacgaaataa
    19861 tccacgacat cctgctcaca caaccagtcg ccacccgcta ctgtatcgtg aaagtgatat
    19921 tcgaagctgt catgatcctg cgcgacagcg gcggagcccc cttctgcagc aacggtatgg
    19981 ctacgcatcg ggtatacttt tgagattagt gcgatttttg catttggatt tgcctgcgcg
    20041 gcagcaattg cagcacgtaa tcccgcgcca ccggcgccta caatggcaag atcggcttga
    20101 aaggtttgca cgacattcct ccagattgtt tttatcccac agccccgtac ttcagggtaa
    20161 gtgcctgaaa gttacggtct gcgaacgcta ttccactgct cctttatagg tacaacagta
    20221 tagtctgatg gataagtctg aaatttgacg agatcgattt ttttagtgcg caagagggct
    20281 aaattatcac tgaagatgat taatttaatt actaaaccat cagatcgtgc tttttttagt
    20341 cactgcccac cgctgtttga ttcctgcgta aaatttgtct cgccgtcgcg ttgcgagtag
    20401 acttcgtgcc cttgtcaaaa actggagatt taactatgag cgaaacggca tcctggcagc
    20461 cgagcgcatc cattcctaac ttattaaaac gcgcggcgat tatggcggag atccgtcgtt
    20521 tctttgccga tcgtggagtg ctggaggtgg agacgccttg tatgagccag gcgacggtaa
    20581 ccgatattca tttggtcccg tttgagacac gtttcgttgg ccccgggcat tcgcagggga
    20641 tgaatctctg gttaatgacc agcccggaat accatatgaa acgcctgctg gttgctggtt
    20701 gtgggccggt attccagctg tgccgcagct tccgtaatga agagatgggg cgttatcaca
    20761 accctgagtt cactatgctg gagtggtatc gaccgcacta tgatatgtac cggttgatga
    20821 acgaggtgga cgatctctta caacaggtgc tggactgccc ggcagcagaa agcctttctt
    20881 atcaacaagc tttcttgcgt tatctggaaa ttgacccgct ctctgccgac aaaacgcaac
    20941 tgcgggaagt cgcagcgaaa ctggatttga gcaatgttgc tgataccgaa gaagaccgcg
    21001 acacgttgct acaattgctg tttacctttg gcgtagagcc aaatattggt aaagaaaaac
    21061 cgacctttgt gtaccacttt ccagccagcc aggcatcact ggcgcaaatc agtaccgaag
    21121 atcatcgggt cgctgaacgc tttgaggttt attataaagg tattgagctg gcgaatggtt
    21181 tccatgaatt aacggatgcc cgtgagcagc aacaacgctt tgaacaagat aaccgtaagc
    21241 gcgcggcgcg cggtttgccg cagcacccca ttgaccagaa tctgattgaa gccttgaaag
    21301 tcggtatgcc tgactgttcc ggcgtggcat taggtgttga tcgtctggtg atgttggcgc
    21361 tgggcgcgga gacactggct gaagtcatcg cctttagcgt tgaccgggca taattctgaa
    21421 aaattactga attaacagcg aagaatggcg tgataagcgc cattctgtag catatgtttt
    21481 catttttccg ttaaaggcca caatttaaga ctggatattt gatatcatcc aggtatcaat
    21541 tctgtattgg tttttacgct gcctgctcaa tgttgcgcag tgcagttcag tgggcacgtg
    21601 ttatacacgc gctgaaatga aggatggttt catgcctcac acgataaaaa agatgagtct
    21661 gataggactc atattgatga tctttacttc cgtatttgga tttgccaata gcccatcggc
    21721 ttattactta atgggttata gtgcgattcc cttttatata ttttctgcat tgttattctt
    21781 tattccattc gccttaatga tggctgaaat gggagctgct tatcgcaaag aagaaggtgg
    21841 tatctattcc tggatgaata atagtgtcgg accacgtttt gccttcattg gtacgtttat
    21901 gtggttttcc tcttatatca tctggatggt gagtacctcc gcgaaagttt gggtaccgtt
    21961 ctcaacattc ctctatggta gtgacatgac ccagcactgg cgtattgccg gactggagcc
    22021 tacgcaggtg gttggtctgc tggcagtagc atggatgatt ctggtcaccg tcgttgcttc
    22081 aaaggggatt aataaaattg cccgcattac tgcggtgggc ggtattgcag taatgtgtct
    22141 gaatttagta ttgctgttag taagcattac tattttgtta ttaaatggtg ggcatttcgc
    22201 gcaggatatg aatttccttg catcaccgaa cccgggttat cagtccggtc tggcaatgct
    22261 atcgtttgtg gtatttgcta tttttgccta tggcggaatt gaagcggttg gtggtctggt
    22321 cgataaaacg gaaaatccag aaaagaactt tgccaaaggt attgtttttg ccgctattgt
    22381 tatttcaatc ggttattcgc tggcaatatt tttatggggc gtcagcacaa actggcagca
    22441 ggtattaagt aatggttccg ttaaccttgg caatattacc tatgtgctga tgaagagcct
    22501 cggggtgacg ctgggtaacg cactgcattt gtcacctgaa gcgtcattgt cgctgggcgt
    22561 atggtttgcg cgtattaccg gactttcgat gttcctcgct tataccggtg cgttctttac
    22621 gctttgctat tcaccgctga aagccatcat ccaggggacg ccgaaagcgt tgtggccgga
    22681 accgatgacg cgcctgaatg cgatggggat gctgtctatc gccatgtgga tgcagtgcgg
    22741 gttggttact gtcttcattc tgctggtttc gtttggtggc ggtaccgcat cggcgttctt
    22801 taacaagttg acgctgatgg cgaacgtgtc tatgacgctt ccttacctgt tcctcgcgct
    22861 ggctttcccg ttttttaaag cacgtcagga tctcgacaga ccatttgtga ttttcaaaac
    22921 gcgtatgtcg gcaatgattg cgacggtggt tgtcgttctg gtggtgacat ttgcgaacgt
    22981 cttcaccatt attcagcctg tggttgaagc cggagactgg gacagcacat tgtggatgat
    23041 tggcggccct gtcttcttct cgctgttagc gatggcgatt taccagaact attgcagtcg
    23101 catggcgaat aaacctgagt tagctctcga ctgatcatat gaaatcctgc aactcatcgt
    23161 ctctccggcg atgagttgtc aggaagtaat gcgtatggat gatactctcc agggccggtg
    23221 ataactgaag atgatgttaa gctgctaaat gagccacgtt gacatcattt ggcagcttca
    23281 tgcattacaa acttcccgcc tgacgacctt tgcccgcaga cgtcagcgtt ctccccgttt
    23341 gtttaccgtt gaggctttcc agacgcatct ggaagggcgg gaatggcata tcgataccgt
    23401 gggcgtggaa gcctgccaga atcagctggt ggatctcatg gcgtagcggc atacggtgac
    23461 ccatctcagc ggcgtaaata cgcagctcga aaatctgaat cccctgttgc agatccacca
    23521 ggaagacttc cggtgccggg ttgtcgatca ccagcgagca gcgacgcgct gcggtgagca
    23581 ggatttccgt cacttcttca ctgttggcat cggcaggggc cggaatcgtc aacactacgc
    23641 gcgtgaccga gtcagagagc gaccagttga taaactgctc ggtaataaac gccttgttcg
    23701 gcacgattat ctctttacgg tcccagtcgc tgatggtggt ggcgcgggtg ttaatttttg
    23761 tcacgctacc agtaagatcg cggatcgtta ccgtatcgcc aatgcgaatc ggtttttcga
    23821 acaggatgat caggccagag ataaagttgg cgaaaatttc ctgcaaacca aaaccgagac
    23881 caacaccgag cgcggcaacc agccactgca atttcgacca ctcaatacca atcattgaga
    23941 agccgaccag cccgccaatc agcatcagca gatattttgt gatggtggtg atggcgtaac
    24001 ccgtacccgg cgttaaatcc aggtgctgca aaatcgccag ttccagcagc gcgggcaggt
    24061 tgcgcaccag ctgcgtggtg atgataaata ccagaatggc aatcagcacc gcaccgaggg
    24121 taattggctc cagactttct acgccctgta ccgtggaggt gacatcccac agcgaaatat
    24181 tttcgaggaa gccgaaagcg gaatggattt ctgaccacag cacaatgacc gaaagcaggg
    24241 cgatcaacat caaaattgag cgcaccagcc gcaaggattg cgcactgatg gcatcgagat
    24301 cgacttcgct ttcatcgact tcaattgctc cttccgggct actgtgatga tgcgcttcct
    24361 cttcgccacg cgcacgttgc gctaacatct ctgcccggcg atgcttcgcc cggtcaaacg
    24421 ccagcctgcg acgctggatc agcatccagc ggcggataac gtgataaacc accagtagca
    24481 ggaaccagat ggcaaccgag gtttcaagcc tcgctaacag tgcctgcgcc gttgccagat
    24541 aacccaccgc cgacgccaga atggcaacca gtggtgcgcc aatcatcatg ttccacagca
    24601 tatggttggt aatgttgtcg ccgctgcctt ctttgttgag atacaacgga ataccggctt
    24661 ttttcaggct tagggtgacc accgccagcg cacagcaaat gaggataaag caaagccgtc
    24721 ccagcgaacc ggaaaattcg cggtcgtcga ggttatcgaa catcatcagc gccataatca
    24781 gcccgatgct catcaggtag tagcgcatcc cacgggaaac acgttcgcgc ggccagccaa
    24841 aatgagcgat aaacaagccg ttcgggcggg caaaggtggc gcaaatcatc actacccaca
    24901 gcatcggcac ggtggccgtt acaccatcgc caatcgcgac cgccagcgga taaggccacg
    24961 cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg taacggtgac gcgacgagaa
    25021 tcgaccagaa aagagtgcgc aacgtcagcc agaagtgatc ctgagtcact ttgccgactt
    25081 tcgccgccga acgttcaaga aaacgggtga aatagcggcg cgagtaaata ctgcaaccga
    25141 ccagaatcaa cgcgccaaac agcggcaaaa ttgtctcttt gctggtcagc atcatcacac
    25201 tggctttgcc caactgactg aaggtgtcca gcgaaatgag acgacgcaga tcctgggcga
    25261 tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga ggtccagaac agatagcggt
    25321 gcgttgcttc gttcacctct ttcagcgcat cctccagttg cccgttggag actttcagct
    25381 tggtcagttc caatagtagc gtgtcgccac cctgcaacaa tgagttcagc aactcacgct
    25441 gagtgcgcag ttgtgcttcc agaatacggt tttgctcggc ggtcagcggc tgaccgtcgg
    25501 cctgatggat ttgccgtagc agcggctgtt tattaagtaa atcctcataa cgtaaccgtt
    25561 gcacacgcaa ctgcgacatt tcggtatcaa gctgttgtgg ttttggcatt tctggtagcc
    25621 gtgccacctg cgcccgcagc gcttcgccga gcaggttgga cgatcccagc cattgcgact
    25681 gttcacgcag cgtattcaac gcctgccgga cctgtaacgt ctggctggca gcctgacgct
    25741 gttgcgaggc aacgagatcc atccgctgcg cctgttgatt cagcgctgcc gatagttcgc
    25801 ggttaatttt gaattgcgcg acgatatctt tcggcaaatc ggcactgttt tctgccagta
    25861 attcggtact ttccagcgcc cgctccgcct caagctgacg ttggctattc aactgattac
    25921 gtaaggcctg caaatacgca tccagttgct ggctctcttt ttccgccagc tctgagcgta
    25981 agcgcgctaa ttcctggcgg ttattggcag acagctgcgc cagttccagt tcatcaacga
    26041 gcgccttaag acgtgcagag tcagactgca acgcgaaatt ttgtgcctga ttgagcggag
    26101 tattgccggt aagcgttccc aggcggcgct cgatctcatt taactgacgg cgggcgtcgg
    26161 tttgctgttg cggcagttga ttcagcgaat cggcaatctc gcgggcgcgc tcctgctctt
    26221 gctgggcctg acggctttta tccagcaact ggctgctgac ctggagaatt tcctgattca
    26281 gcgcgtcggt agacattccc ggcgacacgc tgcgcggctc gtcacgcatg ttgtttaatt
    26341 gtgcgcgcag agtagcggag agtttcggat aattatcgat aacttgctga tattgtttga
    26401 tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa ggcagactgg agcgcctcta
    26461 cgacttccgg ctgtgcgggt ttcgccgctt ttgcctgctc cagttcctga gtgatttgtt
    26521 tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag gcaccaggcc atcagaaaag
    26581 tgataatcag gcgcacgtca gcgtttcctt tgatggatta gacctagtct tttttatcgt
    26641 caaccaatgg gctggcgtcg tgttctgctt cgatctcttc agcaggaagt ggggcgggtt
    26701 cagcgtctgg cgtaacaaag gtttcggtag atactgccag cggctggcca attttcgtga
    26761 cagacaggct ttccagttgc tcaaccagat tcactttacc cggtgcaaac aggttgataa
    26821 cggtggaacc gagtttaaag cgacccattt cctggccttt cagcagtgcc acagaaccgt
    26881 cgttttcccc ggcaggccag gtccagcgct tgatgatacc ttcgcgcggc ggcgtaatgg
    26941 tgcccgccca gaccgtctca atgctgccaa caatcgtcgc tccgaccaga atctgcgcca
    27001 ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc gttacgggca aacagattcg
    27061 gcacgttctg agcggtgaga tggttaacgg aaaagagatc gcccggcacg tagatcatct
    27121 cacgcagaat accgttgcac ggcatgtgta cgcgatggta gtcacgcggg gagaggtaag
    27181 tggtcacaaa cgtaccgttg cggaacaggt ccgccatcag atagttgcat gccagcaggg
    27241 cttcgaggct gtagttgtgg cctttggctt gcaggatttt atcttcttcg attttaccca
    27301 actggctgat aacgccatcg gcaggcatga ccagtacatt cggatcggta tcgattgggc
    27361 gtacttcgtc acgcagcgga cggacaaaga attcgttaaa ggtgcggtag ctggcggtgt
    27421 ccggcttttg cgcctctttc atgtcgacct tgtagtattt aacgaacaga tcgataacca
    27481 gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc cgccaggcga gtaagccata
    27541 gtttcggcag aatgtactgt agcgaaagtt taaatgaatt taacaaggta gcctccaggc
    27601 cattgttttg tcgttcctga tccggccaac atgccggatc ctgaaaaaaa ggggacgatt
    27661 ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga aaagttttta cgcgttttta
    27721 cctgcgccat gctttccaga atacggtgat agttttcgaa acgggtttcc gcgattttcc
    27781 cttcctcaac cgcttcccgg atagcgcagc ccggatcggt atcgtgtttg caatcgcgat
    27841 atttacacag acctaaatag tcatggaatt cgacaaagcc ctgagtgatt tgttccggct
    27901 ccaggtgcca gaggccgaac tcacgcacac caggggaatc aatcacatca ccgccgtgcg
    27961 ggaagtgata cagccgagcg gcggtcgtgg tgtgctgacc gagccccgag ttgtcagaga
    28021 catcgttggt caggatctct ttttgcagcc ccagcaacgc gttcagcagg ctggatttac
    28081 cgacgccaga ctgcccggca aaaatgctga tgcgcccggt caacgcctct tccagcggtt
    28141 tcagcccatc ctgagtatgg ctggaaacca tcaatacgcg ataaccgatg ttgcggtaga
    28201 tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc cagcaggtct atcttgttga
    28261 gcacgataat cggctcaatc tgcaaggttt cgcaggccac caggtaacgg tcgataatat
    28321 tgagcgacag ctccggcaaa atggcggaga caatgacaat ctggtcgatg ttggcggcta
    28381 tgggtttcac gccgtcgtag aaatccggac gcgttaacac cgaggtgcgc tcatgcaccg
    28441 cttccacgat ccctttgaca tttacgcctt ccgccgccgg tttacccgga cgccagacta
    28501 cgcggtcgcc ggttaccagc gaacggatgg tacggcgaat attgcagcgg tgaacgtcgc
    28561 catcggcgga ttccacatca gcgtgcatac caaagcggct gatgacgata ccttcatcag
    28621 gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt agacgtttta agacgacgct
    28681 ggtgattggc gttcacgcgg cgctgctggc ctttggagag tttattttta ctcaatcgta
    28741 ctgactcctg gtcgcccctg atgggcaaaa catctatgat acacgcaatt gtggatcaat
    28801 atagtcactg tgaatgggtg gaaaatagca tgagtgccaa tgaaaacaac ctgatttgga
    28861 tcgatcttga gatgaccggt ctggatcccg agcgcgatcg cattattgag attgccacgc
    28921 tggtgaccga tgccaatctg aatattctgg cagaagggcc gaccattgca gtacatcagt
    28981 ccgatgaaca gctggcgctg atggatgact ggaacgtgcg cacccatacc gccagcgggc
    29041 tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga agctgaactg gcaacgctcg
    29101 aattcttaaa acagtgggtg cctgcgggaa aatcgccgat ttgcggtaac agcatcggac
    29161 aggaccgtcg tttcctgttt aaatacatgc cggagctgga agtctacttc cactaccgtt
    29221 atctcgatgt cagcaccctg aaagagctgg cgcgccgctg gaagccggaa attctggatg
    29281 gttttaccaa gcaggggacg catcaggcga tggatgatat ccgtgaatcg gtggcggagc
    29341 tggcttacta ccgcgagcat tttatcaagc tgtaaaattt ttcctggtca cgtaagcgcc
    29401 ggaatccact tgcggcggcg tgattttgac gctaaatcga acgtcttgct gataatttca
    29461 gcacttgaga taaaaacgca aaaaaaactt ttttgggggg ttgcagaggg aaagatttct
    29521 cgtataatac gcctcccgta acgacgcaga aatgcgaaaa ttacgaaagc aaaattaagt
    29581 agta
//
LOCUS       DADKXM010000037        29307 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.037, whole genome shotgun sequence.
ACCESSION   DADKXM010000037 DADKXM010000000
VERSION     DADKXM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.037"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            390..2048
                     /locus_tag="JQR05_001629"
     CDS             390..2048
                     /locus_tag="JQR05_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY5323821.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2045..3001)
                     /locus_tag="JQR05_001630"
     CDS             complement(2045..3001)
                     /locus_tag="JQR05_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY5323822.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3152..4267
                     /gene="macA"
                     /locus_tag="JQR05_001631"
     CDS             3152..4267
                     /gene="macA"
                     /locus_tag="JQR05_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY5323823.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4264..6211
                     /gene="macB"
                     /locus_tag="JQR05_001632"
                     /pseudo
     CDS             4264..6211
                     /gene="macB"
                     /locus_tag="JQR05_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(6284..6508)
                     /gene="cspD"
                     /locus_tag="JQR05_001633"
     CDS             complement(6284..6508)
                     /gene="cspD"
                     /locus_tag="JQR05_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY5323824.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6831..7151
                     /gene="clpS"
                     /locus_tag="JQR05_001634"
     CDS             6831..7151
                     /gene="clpS"
                     /locus_tag="JQR05_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY5323825.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7182..9458
                     /gene="clpA"
                     /locus_tag="JQR05_001635"
     CDS             7182..9458
                     /gene="clpA"
                     /locus_tag="JQR05_001635"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY5323826.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9861..9948)
                     /locus_tag="JQR05_001636"
     tRNA            complement(9861..9948)
                     /locus_tag="JQR05_001636"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:gga)
     gene            complement(10202..10420)
                     /gene="infA"
                     /locus_tag="JQR05_001637"
     CDS             complement(10202..10420)
                     /gene="infA"
                     /locus_tag="JQR05_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY5323827.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10705..11409)
                     /gene="aat"
                     /locus_tag="JQR05_001638"
     CDS             complement(10705..11409)
                     /gene="aat"
                     /locus_tag="JQR05_001638"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY5323828.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLRR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11451..13172)
                     /gene="cydC"
                     /locus_tag="JQR05_001639"
     CDS             complement(11451..13172)
                     /gene="cydC"
                     /locus_tag="JQR05_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY5323829.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13173..14939)
                     /gene="cydD"
                     /locus_tag="JQR05_001640"
     CDS             complement(13173..14939)
                     /gene="cydD"
                     /locus_tag="JQR05_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY5323830.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15062..16027)
                     /gene="trxB"
                     /locus_tag="JQR05_001641"
     CDS             complement(15062..16027)
                     /gene="trxB"
                     /locus_tag="JQR05_001641"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY5323831.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="JQR05_001642"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="JQR05_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY5323832.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21269
                     /gene="ftsK"
                     /locus_tag="JQR05_001643"
     CDS             17202..21269
                     /gene="ftsK"
                     /locus_tag="JQR05_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY5323833.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALLWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21424..22035
                     /gene="lolA"
                     /locus_tag="JQR05_001644"
     CDS             21424..22035
                     /gene="lolA"
                     /locus_tag="JQR05_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY5323834.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22046..23389
                     /locus_tag="JQR05_001645"
     CDS             22046..23389
                     /locus_tag="JQR05_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY5323835.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23480..24772
                     /gene="serS"
                     /locus_tag="JQR05_001646"
     CDS             23480..24772
                     /gene="serS"
                     /locus_tag="JQR05_001646"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY5323836.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25012..27456
                     /gene="dmsA"
                     /locus_tag="JQR05_001647"
     CDS             25012..27456
                     /gene="dmsA"
                     /locus_tag="JQR05_001647"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAY5323837.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNHTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27467..28084
                     /gene="dmsB"
                     /locus_tag="JQR05_001648"
     CDS             27467..28084
                     /gene="dmsB"
                     /locus_tag="JQR05_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY5323838.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28086..28949
                     /locus_tag="JQR05_001649"
     CDS             28086..28949
                     /locus_tag="JQR05_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY5323839.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28985..>29307)
                     /locus_tag="JQR05_001650"
     CDS             complement(28985..>29307)
                     /locus_tag="JQR05_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY5323840.1"
                     /translation="TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEI
                     TRHSAWDRMSQAGAQLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTL
                     TKQK"
BASE COUNT         7270 a         7246 c         7907 g         6884 t
ORIGIN      
        1 atggtcgttt tatagggtcg tcgtaaaaaa tgacgacgga aataatgcgc ggctatttta
       61 aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt tagggcaaca
      121 gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagca aaagatttaa
      181 tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg ttgtttttgc
      241 gggcttatca gcaagaggga gtataacgcg attattcgct catttttcag acaattgcca
      301 tgcttaaatg tgatgtcatc acgtattagc aaggcctttc ccgttatact gccagcgtaa
      361 aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa attgtgggtt
      421 ttcgcggtat caaccgtttg tcgttgatgc tcgaacaaaa caacgtcctg attggggaga
      481 acgcgtgggg taaatccagc ttgctggacg ccttaaccct gctgctatcg ccagaatcag
      541 atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc aacgggcgag
      601 aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga catcgggttc
      661 gccgttatcg gccgctggaa gcgtgctgga cgccatgcac cgatggctat caccgtattt
      721 tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg cgcagttttc
      781 tcgataaaga cggacatccg attgatgttg aggatattaa cgatcaggca cgccatctgg
      841 tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg
      901 gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc
      961 gtgagttgtc ctcacatccg caaaatctct ctgatggaca gattcgtcag ggactttccg
     1021 caatggtgca gttgcttgag cattatttct ctgagcaggg ggctggacag gcgcgatatc
     1081 gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg gcgctatctg gacatcatca
     1141 accggatgat tgaccgacct ggagggcgct cgtatcgggt tattttgctc ggcctgtttg
     1201 ctactttatt gcaggcaaaa ggcacattgc gactggataa agacgcgcgt ccgttattgc
     1261 taattgaaga tccagaaacc cgcctgcatc cgattatgct ttccgttgcc tggcatctgt
     1321 tgaatctttt gcctttacag cgtattgcta ccaccaactc tggcgagctg ctttcgttaa
     1381 ctccagtgga acatgtttgc cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc
     1441 tggggccaag tggcttgagc accgaagaca gccgccgcat ctcttttcac attcgtttta
     1501 atcgtccgtc atcgctgttt gctcgctgct ggttgctggt ggaaggggaa acggaaacct
     1561 gggttatcaa tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa gggatcaagg
     1621 tcattgagtt tgcccagtcc gggctaaagc cactggttaa atttgcccgc cgaatgggga
     1681 ttgaatggca tgtactggtc gatggcgatg aagcagggaa gaaatatgcc gctacggtac
     1741 gtagcctgtt gaataacgat cgggaagccg aacgagaaca tttaacggcg ttaccggcgc
     1801 tggatatgga acattttatg tatcgccagg gattttccga tgtgttccac cgcgtggcgc
     1861 aaatcccgga aaatgtaccg atgaatctac gcaaaattat ctcgaaagcg atccatcgct
     1921 cttccaaacc cgatcttgcc attgaagtgg caatggaggc aggacgtcgt ggtgtggact
     1981 ccgtaccaac gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg
     2041 cggattaacc gcgaaacatc gtggccattt gtggctgaat agcgtcgagc atctcatagc
     2101 gccgacggta ttcagcccgt tttttactgg cgatttcggc aatctctttt cgtgctatct
     2161 gtgctggaag gcggtaatgg cgttcagcat cacatacgcc gccaaccgac tcccagaacg
     2221 cgttgtaatc agcatggatc ttgccttctt tatcgcgata acgcaggctg cggtaaatat
     2281 gcgtttcatt gctgacggca ataatctgct ctacctgcaa acgttgggca aacagacagg
     2341 ccgcttccat cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc gcattctgga
     2401 tttcctgatg tggaatttcc cattttgcgc cttgcagtcc gccaataaac atcgttcttt
     2461 tcccctgata ttcacacagg gtaaacgtga tctctgccag aggaatacct tcgctgttgc
     2521 ggaacaggat tgtgctgtca ccttctttat ccattgagat catcatggtc agctcaagcg
     2581 tgaactgctc accgtttttg ccttccagtt tagccagttg cagcccgggg gtattcaaat
     2641 ataagctgaa ttcttccgcc gacatacacc cgcggagtaa cgcataatgg taacgtaacg
     2701 cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc agacgcacag
     2761 gcagtcgcgg ctggcgcgtt aacaacacat tgagattagg ccagtgggaa agttcgttca
     2821 tccactcaac gcttaaacgc ggcataatca acgagcgcag caaaaatttc tggcgaaaac
     2881 tacagcgatg ccagaattta cccggccgac actgtccacg tgccagacta agaaaaagtg
     2941 acaggctgct gagagattca gatggcgtaa aggtccgttc agttagctgc gacatattca
     3001 tgaaatcaat ggttatacat gacgtcgatt tcaccattgc gtatcttaac caaacatcaa
     3061 tagtgtgatt actaacgtaa attttagggt tttgttgata tttcgttgaa gttaatgacc
     3121 cggattggca tatggagtat tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg
     3181 ttacgttatt gcgctggtga tagtcatcgc cggactgatt acgttatgga gaattcttaa
     3241 cgcacccgtg ccgacttatc agacactgat tgtgcgcccc ggtgatttac agcaaagcgt
     3301 gctggcgacc ggaaagctgg acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg
     3361 tcagttgaaa acgctgtcgg tggcgattgg cgataaagta aaaaaagacc agcttttagg
     3421 ggtcattgat cctgaacagg ctgaaaacca gatcaaggag gtcgaagcaa cgctgatgga
     3481 gctacgcgcg cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc
     3541 ccgtcagcaa cgtctggcac aaacgcaagc ggtttcactg caggatctcg ataccgccgc
     3601 gacggagatg gctgtgaaac aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa
     3661 tcaggcctct ctcgatacgg ctaaaaccaa tctcgattac acccgtatcg ttgccccgat
     3721 ggccggggaa gtgacgcaaa tcaccactct gcaaggccag acggtgattg ccgcacagca
     3781 agcaccgaac attctgacgc tggcagatat gagcactatg ctggtaaaag cgcaggtttc
     3841 tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga
     3901 tccactgacg cgctacgagg ggcaaatcaa ggatgtgcta ccgacgccgg aaaaggtaaa
     3961 cgacgctatt ttctattacg cccgttttga agtacccaac cccaatggtt tgctgcggct
     4021 ggatatgacc gcgcaagtgc atattcagct caccgatgtg aaaaatgtgt tgacgatccc
     4081 tctgtcggcg ttaggcgatc cggttggcga taatcgttat aaagtcaaat tgttgcgtaa
     4141 tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg atgttgagat
     4201 tgtcaaaggg ctggaagcgg gcgatgaagt ggtgattggt gaggccaaac caggagctgc
     4261 acaatgacgc ctttgctcga attaaaggat attcgtcgca gctatcctgc cggtgatgag
     4321 caggttgagg tgctgaaggg catcagcctc aatatttatg cgggtgagat ggtcgcgatt
     4381 gttggcgctt cgggttccgg taaatcgacc ctgatgaata ttctcggctg cctggataag
     4441 gccaccagcg gcacctatcg cgtagccggt caggatattg ccacgctgga cgccgatgcg
     4501 ctggcgcaac tgcgccgcga gcatttcggc tttattttcc agcgttacca tttgctttcg
     4561 catttaaccg ccgagcagaa cgttgaagta cccgccgtct atgccggtct tgagcggaaa
     4621 cagcgactgc ttcgtgccca ggagttgctg caacggctgg ggctggaaga ccgtacagag
     4681 tattatccgg cacagctttc gggtggtcag caacagcgcg tcagcatcgc gcgggcattg
     4741 atgaacggcg gtcaggtaat tcttgccgat gaaccgaccg gcgcactgga cagccattct
     4801 ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc
     4861 gtcacccacg atccgcaggt cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc
     4921 gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg ttgctggcgg gacggagcct
     4981 gttgtcaata cagtgtctgg ctggcggcag tttgtcagcg gttttaacga ggcgctgacg
     5041 atggcatggc ggacgctggc agcgaataaa atgcgtactt tactgaccat gctggggatt
     5101 attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg
     5161 gtgctggcgg atattcgttc tattggtacg aatactattg atgtctatcc cgggaaagat
     5221 tttggcgatg acgatccgca atatcagcag gcgctgaagt acgacgactt aatcgccatc
     5281 caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt
     5341 tataacaatg ttgatgttgc tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc
     5401 tatggcatga cacttcagtg aaggaaacac ctttaatcag gagcagctga acggtcgtgc
     5461 gcaggtcgtg gttctcgaca gaaatactcg ccgccagctt ttcccccata aagcagatgt
     5521 ggttggcgag gtgattctgg tcggcaatat gcccgccaga gtcattggtg tggcggaaga
     5581 aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctacctt acagcacgat
     5641 gtccgggcga gttatgggcc agtcgtggct taactccatt actgtcaggg tgaaagaagg
     5701 atttgacagc gccgaggcgg aacagcaact cacgcgttta ctttcactgc gccacggtaa
     5761 gaaggatttc tttacctaga acatggacgg cgtcttgaaa actgttgaaa agaccacacg
     5821 tactttacaa ctgtttctga cgctggtggc ggtgatttcg ctggtggtgg gcggtattgg
     5881 tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg gcattcgcat
     5941 ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt
     6001 ttgcctggtc ggtggcgcgt tgggaataac actgtcactg ttaattgctt tcaccttgca
     6061 gcttttctta cccggctggg agattggttt ttcaccgttg gcgctgctgc tggcgtttct
     6121 ctgctcgacg gccaccggga ttttatttgg ctggttaccc gcacgaaatg cggcacgact
     6181 ggatccagta gatgctctgg cacgagagta atttttgaga taaaaatgcc agccgatcgg
     6241 gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctatgcg actgccgctt
     6301 ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg tggacatcaa
     6361 actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg gtggaataat
     6421 gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct ttggcattgt
     6481 tgaaccactt aacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt
     6541 aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa ctctagaaat
     6601 acaatttaga gaattagggc gaaccgtcaa gcatttgaca ggggccaggg ggcaggtatg
     6661 aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt cgctgatgcc
     6721 aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat aagctcttgt
     6781 cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca atgggtaaaa
     6841 cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac
     6901 cgccatctat gtataaagtg atattagtca atgatgatta cactccgatg gagtttgtta
     6961 ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg
     7021 ctgttcacta ccaggggaag gccatttgcg gagtctttac cgccgaggtt gcagaaacca
     7081 aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg tgtacgctag
     7141 aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat caagaactgg
     7201 aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg
     7261 tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg ctggaagcgt
     7321 gttctgtgga tttggttgcg ctccgtcagg aactggaagc ctttattgaa caaaccacac
     7381 ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc gacgctgagt tttcagcgtg
     7441 tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt accggtgcaa
     7501 acgttctggt cgctatcttc agcgaacagg agtcgcaggc ggcatatctg ttgcgtaaac
     7561 atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt aaagacgagc
     7621 cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct ggtggggagg
     7681 aacgtatgga gaatttcacg acgaacctga accagcttgc gcgtgtgggc ggaatcgacc
     7741 cactaattgg tcgtgagaaa gagctggagc gtgctattca ggttctctgc cgtcgccgta
     7801 aaaacaaccc gctgctggtg ggggaatctg gtgtcggtaa aaccgcgatt gcagaaggtc
     7861 ttgcctggcg aattgttcag ggtgatgtgc cggaagtgat ggctgactgt acgatttact
     7921 ctctcgatat cggttctctg ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt
     7981 ttaaagcgtt gctcaagcag ctggagcagg acactaacag catcctgttt attgatgaga
     8041 tccacaccat tatcggtgcg ggtgcagcgt ctggtggtca ggtcgatgcg gctaacctaa
     8101 tcaaaccgtt gctctccagc ggtaaaattc gcgtaattgg ttcgacaacc tatcaggagt
     8161 tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg cttccagaaa attgatatta
     8221 ctgaaccgtc gatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag
     8281 cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga
     8341 aatacattaa cgatcgtcat ctgccggata aagccattga tgttatcgac gaagcgggcg
     8401 ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg
     8461 agtccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag agtgaccgcg
     8521 ataccctgaa aaacctcggc gatcgcctga aaatgctggt cttcggccag gataaagcca
     8581 ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac gaacataaac
     8641 cggtcggttc gttcctgttt gccggtccta ccggggtcgg gaaaacagag gtgacggtac
     8701 agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag tatatggaac
     8761 gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt gatcagggcg
     8821 gtttgctgac cgatgcggta atcaagcatc cacatgcggt gctgctgctg gacgaaatcg
     8881 agaaagcgca tccggacgtg ttcaatattc tgttgcaggt gatggacaac ggtacgctga
     8941 ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg accactaacg
     9001 ccggggtacg ggaaactgag cgcaaatcca ttggtcttat ccaccaggat aacagcaccg
     9061 atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt ctcgacaaca
     9121 ttatctggtt cgatcatctg tcaactgacg tgatccatca ggtggtggat aaattcatcg
     9181 tcgagttgca ggttcagctg gatcagaaag gtgtttctct ggaagtgagc caggaagcgc
     9241 gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg
     9301 tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg
     9361 acggcggtca ggtgacggtt gcgctggata aagagaaaaa tgagctgact tacggattcc
     9421 agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtcag gtaggttggt
     9481 gaagtccgta atctcgtcag gggttacgga ctttttattt atggggtgga acggtgaaaa
     9541 ccctattttt ggaggtgaag gtaagttgtt gataattagt gctgctggaa ggtaaggata
     9601 aaaaagggtg ctgcaggaga atgggatggt tttgctttat taacaacggg ctaaacgtgt
     9661 agtatttgag ttcactgccg tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt
     9721 tgcgaacgcg ttcactgccg tacaggcagc ttagaaattc acaggtaaca tactccaccc
     9781 gcccaccatg ttcactgccg tacagacaga taaaatgcga aaaaaaagct cgcactttcg
     9841 tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg
     9901 tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa attgttttgc
     9961 taccaaacct catgggtggc aacggggcgt tactataggg agttggagta aaacggtcaa
    10021 gaagaatttt aatgataatt attgtttgct catactgtaa acaacttgtg cagtatatct
    10081 acatcgagac aagttatgga cttatacttc caaagtactt catacatatc acaaaataaa
    10141 aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg cggtaaaaca
    10201 atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag
    10261 tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg
    10321 cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa
    10381 gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac
    10441 taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc
    10501 gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc
    10561 aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa
    10621 ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat tataacactc
    10681 tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcacc gtggtaccca
    10741 gaaattattc ggcaatcgtc tgaggcgcat ttgattgaga taattaaggt aatcccggcg
    10801 gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag
    10861 cttaccgcca tgaccgataa attcctcaca gaataccaga agcgccgttt tagacgcatt
    10921 ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca cgccgtacat
    10981 accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatgac cgagttcgtg
    11041 aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct
    11101 ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat agggcgagcg
    11161 tttatgaaat cgcttcatac tacggctgat atgcagtgat tctggccata gcaccgcgcg
    11221 gggatcgggc gaccaccaga ggatggggtc gcctggagaa aaccacggaa aaataccacg
    11281 ctggtaagcc attaacaggc gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt
    11341 aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac
    11401 caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc
    11461 tgcttgaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt accttgctca
    11521 ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc gcgaagtcga
    11581 tgggtgacca ttaacaccgt tttctcacgc atcatttctg caagcaattc aaggatctgg
    11641 ctttcggttg tggcatctaa gccttcggta ggttcatcca gcaacaccag tggcgcatca
    11701 tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg
    11761 ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag accaacgcga
    11821 cgcaagatct ccgacagagc ctcatcacta ctgccaggcg aggcgagtaa cagattatca
    11881 cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga
    11941 gccgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc ctgttgcggg
    12001 tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga
    12061 atcgctatat gttccccggc gtttacctga agagaaatcc ctttaagtgc ctgttgggat
    12121 tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga
    12181 gtttgggtat cagggaaggt gacctccggt ttttgatccg ttaagtcaga gatacgtacg
    12241 gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc cagtgcttca
    12301 aacgcggcta acgcacaaaa gacaaacagg gcaattaatg cgccgggttg agcattgccg
    12361 ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc
    12421 attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc cagccattga
    12481 atttctgtat tctctagttg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca
    12541 gcttgccctt gcagccaggc agtcagttgt tggcgatact gtccgcgaag atgagtcaga
    12601 ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt
    12661 aacataacgc tgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc
    12721 accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag gtaaagatga
    12781 tcgagcgtat caacatccgc caccacgcgg ttgagcaatt cgccctgacg atagcgcgcc
    12841 agtccggcag gggagagggg caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac
    12901 acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc agtacgggtg
    12961 attgcggcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca
    13021 acccccgcaa ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag accgatactg
    13081 gcgagcagcg tcacaattgc cagcacaata ccaagactta acatccattt atgacgttta
    13141 tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt
    13201 aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc
    13261 cggccatcct gcataaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg
    13321 accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca
    13381 ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt
    13441 aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg
    13501 ccaacaggtg tatcaacgcc ttgtggcagg agcggtagaa actcgctgac ccaggcgtta
    13561 tccagcgctg cttgtaattc ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc
    13621 cgcaattttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg tttacgccat
    13681 gattctggtg ataaatcacg taattctatc ccgttgattc gtaatgatcc ctgatatgag
    13741 agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg gccaaccaac
    13801 actgcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt tttaccttcc
    13861 ggcgacgtga taaacagatc ctcggcctca atggtcagcg gatcggtcaa tgctaattcc
    13921 gcctcaccgc gctgcggatg ggcgagcggg gtttccataa acgttttcag actgtcagct
    13981 gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa
    14041 aactctggcg caaggatcag ggccagaaaa cctgcagcca gcgtcacccc agtatcgtag
    14101 tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac cagagcaatt
    14161 gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagtcg tagcacttcc
    14221 attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca
    14281 cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag
    14341 cgagcaagag cgagaaagtt acgccggtta gcatcggcag cccccattcc aaccagcgcc
    14401 ataaacaacg gaattagcgg tgctgtgccc agcagaatga gcgccgcagc ccagttagag
    14461 gggaagatcg ccaccacaat cagcaacggc accgacactg ccagcgccat ttgcggcaga
    14521 tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc
    14581 cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctga
    14641 cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag
    14701 accacccatg cgcgcaatac aaaggtcaga accagtaacg taaagggaag cagcagggct
    14761 tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg
    14821 gcaatgatca atatgccgct tacaaagccc agcagacgag aaatattcag ccaacgttgg
    14881 gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacgaga tttattcatt
    14941 gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa
    15001 ataaaggcga cccatagtcg caaggtgtcg ccttctttac ttttgttact gatttgtaaa
    15061 attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct gccatgcagc
    15121 cagtaccggc cgaagtaatg gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa
    15181 agacgccagg aatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt
    15241 agccgttttc cagttccagc tgcccttcga aaatcgcagt attcgggctg tgaccgatag
    15301 caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc gtatcgcgca
    15361 gacgaacacc agtgacaccc atttgatcgc cggtcacttc ttccagcgta cggttggtgt
    15421 gcagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg attttttccg
    15481 cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta gacagataca
    15541 gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga
    15601 agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaaggct tcttcagagg
    15661 gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt
    15721 cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg ttgatatgat
    15781 caaaaatgat ctcagtttca aacttggtgg catgttcgtg catgcgctcc attaataacg
    15841 gaccggtcag atcgtttgga tcaccaggcc agttttccac ttccgtggtg gtggtcagtt
    15901 ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcgtaga
    15961 cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg
    16021 tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg gaatttacag
    16081 acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag catcgataag
    16141 caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct ttataagccg
    16201 attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac taaaaatcga
    16261 tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga aaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaatatcatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccatacgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gttgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcgttgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacggt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa cagcctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccgcagca gcagtatcag cagccacaac agccggttgc gccacagccg cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcagcagc
    19621 cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc
    19681 agccgcagta tcagcagcca caacagccag ttgcgccaca gccgcaggat accctgcttc
    19741 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca caaaccgacg acgccgctgc
    19801 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    19861 ttgaacaaat ggcgcgtctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    19921 tcgttaatta ctctccgggg ccagttatca ctcgctttga acttaacctg gctcctggcg
    19981 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    20041 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata
    20101 aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc
    20161 cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    20221 tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    20281 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    20341 tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcac ctgttaacgg
    20401 aagtcgttac tgatatgaaa gatgccgcca acgcgctgct ctggtgtgtt aacgagatgg
    20461 agcggcggta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    20521 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    20581 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtc gtgttggttg
    20641 acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc
    20701 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    20761 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    20821 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta
    20881 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    20941 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    21001 agtatgttga tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg
    21061 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    21121 aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21181 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    21241 aagtgctggc cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    21301 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    21361 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    21421 atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    21481 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    21541 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    21601 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    21661 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    21721 acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    21781 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    21841 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    21901 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcagctgaa atcccagcaa
    21961 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    22021 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    22081 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca
    22141 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttgcattc
    22201 tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg
    22261 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg
    22321 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    22381 tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga
    22441 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    22501 actgctttcc cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca
    22561 agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct
    22621 gccagatgat acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt
    22681 aaatacgctg gaaatgatgg cggatatggc cgaagtctat gatagcggta agcgggtcct
    22741 gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg
    22801 cgatcgcttt tacgatctaa tttccgcact gcataagtcg gtacgtggta gcgcacccga
    22861 tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    22921 gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca
    22981 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    23041 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    23101 gtttaaagcc gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt
    23161 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    23221 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    23281 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc
    23341 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    23401 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    23461 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    23521 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    23581 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    23641 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    23701 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    23761 tgaaattcgc gatatcgcgc tgaccattcc taacctgcct gcagatgaag tgccggtagg
    23821 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    23881 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc
    23941 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    24001 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    24061 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    24121 tggcgatctg tttcatactc gtccgctgga agaagaagca gacaccagta actatgcgct
    24181 gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    24241 tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata
    24301 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    24361 gattgtgcgc ccagaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    24421 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    24481 ctttggcgct tgcaaaactt acgacttgga agtatggatc ccggcacaga acacctaccg
    24541 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    24601 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    24661 tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga
    24721 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    24781 ttttctgaat ctaaaaagcg cctttagggc gctttttttg tctccctttg ataccgaaca
    24841 ataattactc ctcacttaca cgtaatacta ctttcgagtg aaaatctacc tatctctttg
    24901 attttcaaat tattcgatgt atacaagcct atatagcgag ctgctataga aataattaca
    24961 caatacggtt tgttactgga atcaatcgtg atcaagcttg agtgagccat tatgaaaacg
    25021 aaaatccctg atgcggtatt ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca
    25081 gcgatcggcg gcctggcaat ggccagcagc gcattaacat taccttttag tcggattgcg
    25141 cacgctgtcg atagcgccat tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt
    25201 acagttaact gtggtagtcg ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa
    25261 tatgtcgaaa cggacaatac cggcgatgac aattacgacg gcctgcacca ggttcgcgcc
    25321 tgcctgcgtg ggcgttccat gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg
    25381 atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc
    25441 tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc
    25501 tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg gccgccggga
    25561 aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca ttacggtgac
    25621 tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg ggcagatggt
    25681 aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa taacccaggc
    25741 gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg ccagaaatct
    25801 aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat
    25861 gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct ggcgtacgtc
    25921 atgatcactg aaaacctggt ggatcaggca ttcctcgata aatattgcgt tggctatgac
    25981 gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta tattctgggt
    26041 gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac cggtgttccg
    26101 gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc gtttatcagc
    26161 cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc tatctcgatg
    26221 ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc gcgtgaaggt
    26281 tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca gaccagcatt
    26341 tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc gctgcgtgat
    26401 ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt
    26461 aactgcctga ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct ccaggatgat
    26521 aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct
    26581 gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact ggatgcatcc
    26641 tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt
    26701 aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt
    26761 actgaaggcc gtacccagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg
    26821 attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac
    26881 ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc aaacccactg
    26941 actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat tgccgctacc
    27001 tgggaattgc cagaaggcga tgtgatcgat ccactgccga tttacacgcc gggctttgaa
    27061 agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt ccactataaa
    27121 tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcggcttg ccgtcaggaa
    27181 atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga taaagtcagg
    27241 atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg aatgatgccg
    27301 ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg tgtcgataag
    27361 ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg
    27421 tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga caacccagta
    27481 tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa
    27541 agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat atgctggcgg
    27601 cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat
    27661 ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca
    27721 taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct gccgttactg
    27781 tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa
    27841 atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg
    27901 cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac atggcgatct
    27961 ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc
    28021 caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc cgaaggaggt
    28081 gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat
    28141 gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag
    28201 aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg ggcattggct
    28261 ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg ctcaaccggg
    28321 taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt gctgtaggcg
    28381 gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt acgctatggc
    28441 tggtggtgac gatggttctt ggcgtcgtct ttgtctggat gatggtgcgt gtgtataaca
    28501 gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggcttc ttcctgacga
    28561 tgttcatggg cggcccgtta ctgggttacc tgttgctgag tctggctggc gtcgatggct
    28621 gggcgatgcg tctgctgcca gccatttctg tactggcact ggtggtgagt ggcgtgatgt
    28681 cagtgatgca gggcacagag ctggcaacta ttcatagttc tgtgcagcag gccgcagcgc
    28741 tggtgccgga ctatggtgcg ctgatgtcct ggcggatcgt gcttttggcc gttgccctgt
    28801 gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc ggttccgcta ctatccgtct
    28861 cattcattct gcttctggca ggggaattaa tcggtcgcgg cgtattctat ggtttgcata
    28921 tgaccgtggg gatggccgtc gcaagctaat cagtaacaac cggggtttcg gccccggttc
    28981 tcttttattt ctgcttcgtt aacgtgtcat aactggtcat caaattacga taatcaggaa
    29041 tatggttaga gaacaacgtc gccagtcctt caatatcatt acgccagtca cggtgcagct
    29101 cgcaagccac gccgaaccat gtcatcaatt gcgcaccagc ttgcgacatg cgatcccacg
    29161 ccgaatgccg ggtaatttca ttaaaggtcc ctgatgcatc agtcacgaca aagacatcaa
    29221 acccttcttc aatggctgaa agcgccggga atgccacgca aacttcggtt accacaccgg
    29281 caataattaa ctgtttttta cctgtcg
//
LOCUS       DADKXM010000038        29176 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.038, whole genome shotgun sequence.
ACCESSION   DADKXM010000038 DADKXM010000000
VERSION     DADKXM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.038"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="JQR05_001651"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="JQR05_001651"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY5323841.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="JQR05_001652"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="JQR05_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY5323842.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDVVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="JQR05_001653"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="JQR05_001653"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY5323843.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNTQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="JQR05_001654"
     CDS             complement(3100..6843)
                     /locus_tag="JQR05_001654"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY5323844.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="JQR05_001655"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="JQR05_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY5323845.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="JQR05_001656"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="JQR05_001656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY5323846.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="JQR05_001657"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="JQR05_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY5323847.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="JQR05_001658"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="JQR05_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY5323848.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="JQR05_001659"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="JQR05_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY5323849.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /locus_tag="JQR05_001660"
     CDS             complement(13382..14077)
                     /locus_tag="JQR05_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY5323850.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="JQR05_001661"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="JQR05_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY5323851.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="JQR05_001662"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="JQR05_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY5323852.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="JQR05_001663"
     CDS             16240..16347
                     /locus_tag="JQR05_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY5323853.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="JQR05_001664"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="JQR05_001664"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY5323854.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="JQR05_001665"
     CDS             complement(17386..18195)
                     /locus_tag="JQR05_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY5323855.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="JQR05_001666"
     CDS             complement(18199..18591)
                     /locus_tag="JQR05_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY5323856.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="JQR05_001667"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="JQR05_001667"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY5323857.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="JQR05_001668"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="JQR05_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY5323858.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="JQR05_001669"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="JQR05_001669"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY5323859.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="JQR05_001670"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="JQR05_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY5323860.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVITPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="JQR05_001671"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="JQR05_001671"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY5323861.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAYPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /locus_tag="JQR05_001672"
     CDS             23640..24587
                     /locus_tag="JQR05_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY5323862.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="JQR05_001673"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="JQR05_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY5323863.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSTLVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="JQR05_001674"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="JQR05_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY5323864.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="JQR05_001675"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="JQR05_001675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY5323865.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="JQR05_001676"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="JQR05_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY5323866.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         7007 a         7693 c         7332 g         7144 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acaacatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactggg cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttactgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct gggtgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccgccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacgact ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaggt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccag gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atggccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac gatccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagatgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccagg taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgtcggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg gtatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccttggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtaa ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt ccagcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacgggacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gcgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 gaccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaccgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgattttct ggcacgacct ggcagcaccg atcctggcgg gaattattac cgcagcgatt
    16321 gtcggctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 aacggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatacgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcggcaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttatca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgtacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cgcgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cttggaaagg atcatgccag
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg caccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctactctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct agagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgccttc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatcgcc
    28321 aacgtcaacg aagacggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatcct ggcaagatga
    29161 aaatgataac cagtag
//
LOCUS       DADKXM010000039        29154 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.039, whole genome shotgun sequence.
ACCESSION   DADKXM010000039 DADKXM010000000
VERSION     DADKXM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.039"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..213)
                     /locus_tag="JQR05_001677"
     CDS             complement(<1..213)
                     /locus_tag="JQR05_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY5323867.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JQR05_001678"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JQR05_001678"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY5323868.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2401..2853
                     /gene="nudB"
                     /locus_tag="JQR05_001679"
     CDS             2401..2853
                     /gene="nudB"
                     /locus_tag="JQR05_001679"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY5323869.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2882..3622
                     /locus_tag="JQR05_001680"
     CDS             2882..3622
                     /locus_tag="JQR05_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5323870.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3657..4178
                     /gene="ruvC"
                     /locus_tag="JQR05_001681"
     CDS             3657..4178
                     /gene="ruvC"
                     /locus_tag="JQR05_001681"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY5323871.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQAGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4180..4782)
                     /locus_tag="JQR05_001682"
     CDS             complement(4180..4782)
                     /locus_tag="JQR05_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY5323872.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4853..4918
                     /locus_tag="JQR05_001683"
     CDS             4853..4918
                     /locus_tag="JQR05_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323873.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5057..5668
                     /gene="ruvA"
                     /locus_tag="JQR05_001684"
     CDS             5057..5668
                     /gene="ruvA"
                     /locus_tag="JQR05_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY5323874.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            5677..6687
                     /gene="ruvB"
                     /locus_tag="JQR05_001685"
     CDS             5677..6687
                     /gene="ruvB"
                     /locus_tag="JQR05_001685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY5323875.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6834..7619)
                     /gene="znuB"
                     /locus_tag="JQR05_001686"
     CDS             complement(6834..7619)
                     /gene="znuB"
                     /locus_tag="JQR05_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY5323876.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7616..8371)
                     /gene="znuC"
                     /locus_tag="JQR05_001687"
     CDS             complement(7616..8371)
                     /gene="znuC"
                     /locus_tag="JQR05_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY5323877.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8450..9382
                     /gene="znuA"
                     /locus_tag="JQR05_001688"
     CDS             8450..9382
                     /gene="znuA"
                     /locus_tag="JQR05_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY5323878.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9398..10720
                     /gene="mepM"
                     /locus_tag="JQR05_001689"
     CDS             9398..10720
                     /gene="mepM"
                     /locus_tag="JQR05_001689"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY5323879.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10840..11811
                     /gene="lpxM"
                     /locus_tag="JQR05_001690"
                     /gene_synonym="msbB"
     CDS             10840..11811
                     /gene="lpxM"
                     /locus_tag="JQR05_001690"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5323880.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11942..13384)
                     /gene="pyk"
                     /locus_tag="JQR05_001691"
     CDS             complement(11942..13384)
                     /gene="pyk"
                     /locus_tag="JQR05_001691"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY5323881.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13512..14381)
                     /locus_tag="JQR05_001692"
     CDS             complement(13512..14381)
                     /locus_tag="JQR05_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY5323882.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14718..16193
                     /gene="zwf"
                     /locus_tag="JQR05_001693"
     CDS             14718..16193
                     /gene="zwf"
                     /locus_tag="JQR05_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY5323883.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16428..18239
                     /gene="edd"
                     /locus_tag="JQR05_001694"
     CDS             16428..18239
                     /gene="edd"
                     /locus_tag="JQR05_001694"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY5323884.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18276..18917
                     /locus_tag="JQR05_001695"
     CDS             18276..18917
                     /locus_tag="JQR05_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY5323885.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18973..20151)
                     /gene="purT"
                     /locus_tag="JQR05_001696"
     CDS             complement(18973..20151)
                     /gene="purT"
                     /locus_tag="JQR05_001696"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY5323886.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20285..20575
                     /gene="yebG"
                     /locus_tag="JQR05_001697"
     CDS             20285..20575
                     /gene="yebG"
                     /locus_tag="JQR05_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAY5323887.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20605..20997
                     /gene="yebF"
                     /locus_tag="JQR05_001698"
     CDS             20605..20997
                     /gene="yebF"
                     /locus_tag="JQR05_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY5323888.1"
                     /translation="MLSPESLCYGEKHEKRGAFLGRLLVSACASVFAANNETSKSVTF
                     PKCEGLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVP
                     LTVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            21324..21983
                     /locus_tag="JQR05_001699"
     CDS             21324..21983
                     /locus_tag="JQR05_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY5323889.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQVIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22192..24252
                     /gene="ptrB"
                     /locus_tag="JQR05_001700"
     CDS             22192..24252
                     /gene="ptrB"
                     /locus_tag="JQR05_001700"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY5323890.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQGPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24249..24911)
                     /gene="exoX"
                     /locus_tag="JQR05_001701"
     CDS             complement(24249..24911)
                     /gene="exoX"
                     /locus_tag="JQR05_001701"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY5323891.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24935..25591)
                     /locus_tag="JQR05_001702"
     CDS             complement(24935..25591)
                     /locus_tag="JQR05_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY5323892.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIPGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(25693..25923)
                     /locus_tag="JQR05_001703"
     CDS             complement(25693..25923)
                     /locus_tag="JQR05_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY5323893.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26094..26435
                     /gene="yobA"
                     /locus_tag="JQR05_001704"
     CDS             26094..26435
                     /gene="yobA"
                     /locus_tag="JQR05_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY5323894.1"
                     /translation="MAILTTSLVTPSVWAHAHLTHQYPAANAQVTAAPQAITLNFSEG
                     VETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYTVDWHVVSVDGH
                     KTKGHYTFSVK"
     gene            26439..27311
                     /gene="copD"
                     /locus_tag="JQR05_001705"
     CDS             26439..27311
                     /gene="copD"
                     /locus_tag="JQR05_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY5323895.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLMIRRLLTKRSL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            27324..27665
                     /locus_tag="JQR05_001706"
     CDS             27324..27665
                     /locus_tag="JQR05_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY5323896.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28058..29134)
                     /locus_tag="JQR05_001707"
     CDS             complement(28058..29134)
                     /locus_tag="JQR05_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5323897.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         7358 a         7174 c         7511 g         7111 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagatggg atgagcgaag gcagtcaacg aagaggcagc
     2401 gtgaaggata aagtgtataa gcgtcccgtt tcgatcttag tggtcatcta tgcacaagat
     2461 acgaaacggg tgctgatgtt gcagcggcgt gacgatcccg atttctggca gtcggtaacc
     2521 ggcagcgtgg aagagggtga aaccgcgccg caagctgcca tgcgcgaagt aaaggaagag
     2581 gtcaccattg atgttgtcgc tgaacaactg accttaattg actgtcagcg cacggtagag
     2641 tttgaaattt tttcacattt acgtcatcgc tatgcgccgg gcgtgacgcg taatacggaa
     2701 tcatggttct gtcttgcgct tccgcacgag cggcagatcg ttttcactga acatctggct
     2761 tacaagtggc ttgatgcgcc tgcggcggcg gcgctcacta agtcctggag caaccggcag
     2821 gcgattgaac agtttgtaat taacgctgcc tgaacaggca gggctatctg gagatatttt
     2881 tatggcaggt catagtaaat gggccaacac cagacatcgt aaagctgcgc aggatgctaa
     2941 gcgcggtaaa atcttcacta aaatcattcg tgagctggta accgcggcta agctgggcgg
     3001 tggcgatccg gacgctaacc cgcgtctgcg tgcggcggtg gataaagcac tgtctaacaa
     3061 catgacccgt gacacactga accgcgcaat tgcacgtggt gtgggcggtg atgatgatgc
     3121 aaacatggaa accatcatct acgaaggtta cggtcctggc ggcacggcaa tcatgattga
     3181 atgtctgtct gacaaccgca accgtaccgt tgctgaagtg cgtcacgcat tcagcaaatg
     3241 tggcggtaac ctcggcactg acggttccgt agcttatctg ttcagcaaaa aaggcgtgat
     3301 ctccttcgag aaaggcgacg aagacaccat catggaagca gctctggaag caggtgctga
     3361 agacgttgtg acatatgatg acggcgcgat tgatgtctac actgcatggg aagaaatggg
     3421 taaagtgcgc gacgctctgg aagcggcagg tctgaaagca gacagcgcgg aagtttccat
     3481 gatcccgtcc accaaagctg atatggatgc agaaaccgcg ccgaaactga tgcgtctgat
     3541 cgatatgctg gaagattgcg acgacgtgca ggaagtttac cataacggtg aaatctctga
     3601 tgaggttgca gcgactctct gatgatgcct gctaaacagc aaaacggaga cgcgtgatgg
     3661 ctattattct cggcattgac ccgggttcgc gcgtgaccgg ctacggcgtc atccgccagg
     3721 caggtaggca actgtcctac ctgggtagcg gatgcatacg caccaaagtg gatgatttac
     3781 cgtctcgtct gaagctcatc tatgcgggcg tgacggaaat catcacccag ttccagcctg
     3841 attatttcgc cattgaacaa gtctttatgg cgaagaacgc tgactcagcc ctgaaactgg
     3901 gccaggcgcg cggcgtggcg attgttgcgg cggtgaatca ggaattgccg gtatttgaat
     3961 acgcggcacg tcaggtaaag caaacggtgg taggtatggg tagtgccgaa aaaagccagg
     4021 tgcagcatat ggtccgcacc ttgctgaaac tgcccgctaa tccacaggcg gatgccgccg
     4081 atgcgctggc gattgccatc acccactgcc acgtaagtca gaatgcgatg cagatgagcg
     4141 aatcgcggct gaacctggcg agagggcgac tgcgttaagt tataccgcct cggtcagttc
     4201 cggctgaggc gtttcaactc cctccgcgtg aatcaacacc aaacccggat gatgccacaa
     4261 actggctggc gtgacggttt tacgctccca cggaatagaa cctaagaacc agaatttcca
     4321 gaaagtgagt tttgcattcg gattgctatt taacagttgt ccaaacgtct ctatttcacc
     4381 caccggaata catagcgcct ctttataaat atcaaagaca aattttgaac agaactggcg
     4441 cgaagattcg tatttaaaac ccgtgtggta aagtttgcgt agccgggagg gaacctgttc
     4501 aacaattcgt tgtttttgtt gttctgtcag cccagcgtct aatcgcttta tagcatagcg
     4561 ttgattagac gaacgtttaa taaaacgaga tagcgtggtg atggttgaaa ggggaacccg
     4621 gctttctgca accaggaagt cttcaccgtt atgaccgata atgatcccga cgtgattact
     4681 ccagcaattt gatgcagctg atatttgacc aaataaggca gcacctatac atgtaaagac
     4741 gatatctcca atttcatatt cagcagggta attaatattc acgttgtcat tccattgaaa
     4801 tagatacgaa tatacgtaat agcataaaaa aattattttt tcaggagtcg caatgtatat
     4861 ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc caatataacc
     4921 tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc aagttttctt
     4981 catccttcgc tggatatcta tccagcattt ttttatcata cagcattatc tttgattcat
     5041 tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa aacaaccccc
     5101 gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga tgacctgttt
     5161 ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg tggtgcgtga
     5221 agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt tcaaagagtt
     5281 gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg gaatgtcagc
     5341 gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga aactgccggg
     5401 tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat ttaaaggttt
     5461 gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg ccagcccggc
     5521 gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct ataaaccaca
     5581 agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac gccagcagtg aaacattaat
     5641 tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga ccgtctgatt
     5701 tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc taaattactg
     5761 gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat caaagcagcg
     5821 aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg gttgggtaaa
     5881 actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac gacttctggt
     5941 ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga accgcatgac
     6001 gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt gctgtacccg
     6061 gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc acgctccatt
     6121 aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg ttcgctgaca
     6181 tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca ggtgccggat
     6241 ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag tgatgacggt
     6301 gcgctggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg tctgctgcgt
     6361 cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga tatcgctgct
     6421 caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga ccgcaaattg
     6481 ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa cctggcggca
     6541 gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt gattcagcaa
     6601 ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg gaatcacttt
     6661 ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc atccgacact
     6721 ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag atagcgaacc
     6781 gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat agattagctg
     6841 gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac caccgacgga
     6901 cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc caccatcccc
     6961 accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg cgcagtagct
     7021 gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat cgctacacca
     7081 atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt cacaccatca
     7141 acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg ccagaacaaa
     7201 atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg cgtcactgcc
     7261 agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat cagactaacg
     7321 accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt gtcgatcgcc
     7381 agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt aacggcaatc
     7441 accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag taatgaggca
     7501 tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga acccagcgga
     7561 cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa ttcaatcatg
     7621 agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga tgattatgat
     7681 gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa ataaactccg
     7741 gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc aggcacagca
     7801 cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa acgccacaat
     7861 ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca tttacatcca
     7921 cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat gctcgcgcta
     7981 atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta atcaggtgcc
     8041 cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca gggcgtaagc
     8101 gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc tgcggtacat
     8161 agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt accagcccga
     8221 gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa gtcaaaattt
     8281 ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca aaagaaaccg
     8341 agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag tagtcatgaa
     8401 atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta tgttacataa
     8461 aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa cacaggccgc
     8521 agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg ccattgctga
     8581 tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg attattcact
     8641 gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg ttggcccgga
     8701 gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc aggtaacgat
     8761 tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg atgatgatga
     8821 tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca acatgcatct
     8881 ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa aattagtgga
     8941 acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg aggcacaatt
     9001 ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga aaggttattt
     9061 cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac cgcttggtca
     9121 ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa taagaacaca
     9181 gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc cagcggtcgt
     9241 tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc tggggacgaa
     9301 tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca accagtatgc
     9361 gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag cccgctctgt
     9421 cgccctggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt cgctcaccgt
     9481 tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg ccacgccaat
     9541 tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg aagccagtga
     9601 gccgattgat caagctgcac aagaagatga agccattccc caggacgaac tggatgacaa
     9661 aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata cgctaagcag
     9721 cattctcaat cagtatggta ttgatatggg tgatatcacc caactggctg ctgccgacaa
     9781 agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg cggacggcga
     9841 actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg accgtactgc
     9901 cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta acaatctgct
     9961 gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt taaccagcgc
    10021 cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca aactgaaaaa
    10081 aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac gtgagcaaag
    10141 ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa tccgcgctga
    10201 ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct tgcgattccc
    10261 gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta atccggtgac
    10321 aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta cgccagtgct
    10381 ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag gttattatgt
    10441 ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca agattctggt
    10501 gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta ataccggacg
    10561 ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg taaacccgct
    10621 gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg aattcctggc
    10681 gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca ttcgcagccg
    10741 gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgcgcc gctacactat
    10801 caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa aaaaaataat
    10861 agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg gggagcatgg
    10921 ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg tgatcccatt
    10981 ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg tcgtgcgtta
    11041 atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc gattgttgat
    11101 gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc aatacgcggg
    11161 ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga agagatgcgg
    11221 cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga tattcctgcc
    11281 atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca gggcaacccg
    11341 gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct gcatgcgaga
    11401 aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg atattattta
    11461 cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc cacctataaa
    11521 gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt tgtaccgctg
    11581 tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc accgatggat
    11641 gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga agtcgagatt
    11701 tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa aactcgcaaa
    11761 ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata aaaaagcctc
    11821 tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat attcgaaatc
    11881 tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc cggcaacgta
    11941 cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc atcacgtcgc
    12001 cctgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc agcagattaa
    12061 ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc ggcgtaacgc
    12121 cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg aaaattggta
    12181 gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc atggtaatga
    12241 tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca atagcttctt
    12301 ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc gggatttttt
    12361 ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac tgcccagcgg
    12421 cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt gctacgtcca
    12481 tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc tgggtcgccg
    12541 tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga atgccgacca
    12601 gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc acgtcagagg
    12661 cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt tcaaccttgg
    12721 caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag ttcagatctt
    12781 cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc gcagtcttaa
    12841 tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca agtttgttga
    12901 tacctttatt gttggaaagg ggaccaccga cggtgacttc ggtgaacact ttcatgccct
    12961 gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg tcaccaggca
    13021 cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg ccttcacctt
    13081 tacctaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact ttgccttctt
    13141 taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc acatgacgcc
    13201 ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct tcaggcgagc
    13261 cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt tcaagattat
    13321 tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga agccttttgg
    13381 acatgtaata ctccgttgac tgaaacaact aggtgttgct tgaacatgaa atccgaatga
    13441 aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga ttaggacagt
    13501 gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa acgcgattcc
    13561 ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg tcgcaaagta
    13621 aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac catgggcata
    13681 taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc ccgggcgagc
    13741 ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc cagctgcgcc
    13801 agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac gtctccgtcg
    13861 ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac caccggaaca
    13921 ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga gcctaatccg
    13981 aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat ggcagattta
    14041 tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat tttccccgtg
    14101 tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt gccattcgcc
    14161 agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat gctgcgacag
    14221 aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc aatactcgaa
    14281 tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc tgatttgctc
    14341 aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag agactcacgg
    14401 gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat attacgcctg
    14461 tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat aacatgatca
    14521 gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc cgcactttgc
    14581 gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa gctcgtaaaa
    14641 gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac tgggttagtt
    14701 aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac ctggtcattt
    14761 tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat caactggaaa
    14821 aagccggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct gactgggata
    14881 aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa gaaaccattg
    14941 atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc gatgtcaatg
    15001 acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt atcaccatta
    15061 actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt ggcgaggcaa
    15121 aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg tcgctggcga
    15181 cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag gtttaccgta
    15241 tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt tttgctaact
    15301 ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt accgtggcag
    15361 aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag atgcgtgaca
    15421 tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg ccatctgacc
    15481 tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg cgccgcatcg
    15541 accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc ttcgcccagg
    15601 gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc aatacagaaa
    15661 ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg ccattctacc
    15721 tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat ttcaaaacac
    15781 ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa ctgactatcc
    15841 gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct ggccttgacc
    15901 acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc tttaatcaga
    15961 cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt attcaggcac
    16021 tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc attactgagg
    16081 cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg ggacccgttg
    16141 cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag taatatctgt
    16201 gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt agaacaattt
    16261 ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac actgactgaa
    16321 acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct ctgaatgaaa
    16381 cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg aatccacaat
    16441 tgttacgcgt aacaaatcga atcattgaac gttcgcgcga gactcgctct gcttatctcg
    16501 cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca tgcggtaacc
    16561 tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa agcatgttgc
    16621 gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac cagccttatg
    16681 aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt ggtcaggttg
    16741 cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga atggaattgt
    16801 cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc cataacatgt
    16861 ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg acgatggcag
    16921 ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg gcaagcggtt
    16981 tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa gtggaccgca
    17041 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
    17101 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
    17161 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
    17221 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
    17281 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
    17341 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
    17401 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
    17461 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
    17521 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
    17581 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
    17641 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
    17701 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
    17761 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
    17821 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
    17881 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
    17941 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
    18001 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
    18061 gtgtgaatgg acagacaggc gaactgacgc tgctggtaga cgaagcggaa ctggctgctc
    18121 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
    18181 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
    18241 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
    18301 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
    18361 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
    18421 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
    18481 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
    18541 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
    18601 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
    18661 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
    18721 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
    18781 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
    18841 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
    18901 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
    18961 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
    19021 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
    19081 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
    19141 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
    19201 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
    19261 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
    19321 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
    19381 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
    19441 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
    19501 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
    19561 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
    19621 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
    19681 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttc
    19741 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
    19801 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
    19861 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
    19921 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
    19981 atgggagcga tgcgcaacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
    20041 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
    20101 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
    20161 cccctgatta attccgcgcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
    20221 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat cacagttatt atgagaggct
    20281 gcaaatggcg gttgaagtta aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
    20341 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
    20401 cacctggctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
    20461 atggctggca gaacaaaaag atgtgctgag caccattctg aaaaccggca aattaccgtc
    20521 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
    20581 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
    20641 catgaaaaaa gaggggcgtt tttagggcgg ttgttggttt ctgcctgcgc atcagttttc
    20701 gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg tctggatgct
    20761 gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa atcgcgtggc gcgctgggca
    20821 gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct gggtcagttt gcaggacatt
    20881 cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag tgccgatatt
    20941 cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg gcgttaatgg
    21001 cttctgataa ttcgattgac cgttggcgga tgcggcgcga gtgccttgtc cgccccagaa
    21061 cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg catcggacaa tcagcatcaa
    21121 acaatgccac tcaaaacgac aaactacttt cacctaaaat acccttatca cctgatatac
    21181 agtaaatacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc cgcttatttg
    21241 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
    21301 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
    21361 ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg attgggcaaa ttgttagtgc
    21421 caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cgtaaacttc
    21481 ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtagcg ggtacggtgc
    21541 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
    21601 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
    21661 ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc aatcgaccag caattgcgtg
    21721 aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggtaatcgaa caaccgctgg
    21781 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
    21841 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
    21901 gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat tgaacgcgat ctcgaacagc
    21961 aaaaacgcac tttggcggaa taattccgct ctctggatag cctgaagttg actatttcgg
    22021 gctatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc ataacattcg
    22081 atatccgcgc cattacctgt catcatctaa gcaatgacga ccctgtttcg cttgcatccc
    22141 cggtgagttt tgccaccctt ataagatgtt tcaaccagaa agaacaataa catgctacca
    22201 aaagccgccc gcattcccca cgccatgacg cttcatggcg atacgcgcat cgataattac
    22261 tactggctgc gggacgatac gcgttctcag ccagaagtcc tggactacct gcaacaagaa
    22321 aatagttacg gtcatcgggt gatggcctca caacaagcct tgcaggatcg catcttaaag
    22381 gaaatcatcg accgcattcc gcaaagagaa gtttctgcgc cctacataaa aaatggctac
    22441 cgctatcggc atatttatga accaggctgt gaatatgcta tctaccagcg tcaatcggca
    22501 ttcagcgaag agtgggatga gtgggaaaca ttgctcgatg ccaacaagcg cgcagctcat
    22561 agtgagtttt attcgatggg cggaatggcg attacgcccg ataacaccat tatggcgctg
    22621 gcagaagatt ttctttcccg acgccagtac ggcattcgtt ttcgtaatct ggaaacaggt
    22681 aactggtacc cggaactgct ggataacgtt gaacccagct ttgtctgggc aaatgactcc
    22741 tggactttct actatgttcg caagcatccg gtgacgctgc tgccttatca ggtctggcgt
    22801 cacgccatcg gtacgccagc atcgcaagat acactgatct acgaagaaaa agacgatacc
    22861 tattatgtca gcctgcataa aacgacgtcg aagcactatg tcgtcattca tctggccagc
    22921 gccaccacca gtgaagttcg cctgctggac gcggaaatgg ccgatgccga gccgtttgtt
    22981 tttctgccgc gccgcaaaga tcacgaatac agccttgatc actaccagca tcgtttttat
    23041 ctgcgttcca atcgccacgg caaaaacttt ggcttatacc gtacccgtat gcgtgatgag
    23101 caacagtggg aagagttaat tccgccacgc gaaaacatca tgctggaagg gtttacgctg
    23161 tttaccgact ggctggtggt tgaagagcgt cagcgcgggt taaccagttt gcgccaaatt
    23221 aaccgcaaga cccgggaagt cattggcatt gcctttgatg atccggccta tgtgacctgg
    23281 attgcctaca atccagaacc tgaaaccgcg cgattgcgtt atggttattc ttccatgacc
    23341 acaccagaca ctttgtttga actggatatg gataccggtg agcgtcgtgt attaaaacaa
    23401 acagaagtgc ccggttttga tgcggcgaat taccgcagtg aacacctgtg gatagtcgcc
    23461 cgtgatggcg tcgaagttcc ggtttcgctg gtctatcatc gcaaacattt tcgcaaagga
    23521 cacaacccgt tgctggtgta tggctatggt tcttacggcg caagtattga tgccgatttc
    23581 agttttagcc gcttgagttt gttagatcgt ggctttgtct acgccattgt ccatgttcgc
    23641 ggcggtggtg agctggggca acaatggtac gaagacggta aatttctgaa gaagaaaaat
    23701 acgtttaatg attatcttga tgcctgcgat gcattgttaa aactgggcta tggctctcct
    23761 tcgctctgtt atgcgatggg cggaagtgcg gggggcatgt tgatgggcgt tgcaattaat
    23821 caacgcccgg aattattcca cggcgttatc gcccaggtac cgtttgttga tgttgtaaca
    23881 acgatgcttg atgaatcaat tcctcttacc actggtgagt ttgaagagtg ggggaatccg
    23941 cagggtccgc aatattacga gtacatgaaa agctacagcc cgtatgacaa cgtcaccgca
    24001 caggcttatc cgcatttact ggtaacgacc ggtttacacg attctcaggt gcaatattgg
    24061 gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa ccgatgacca tcttttattg
    24121 ctctgtaccg acatggactc aggccatggc ggtaaatctg gccgctttaa atcgtacgaa
    24181 ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc agggaacatt acccgctacg
    24241 cctgcggatt aagtattttc cagataatgt ttcagtgtta aacgcagctc cgggctcatg
    24301 ctgtccaggt tattaaataa ccagcgcaga tagcccggat cgcgttcggc aacgtcggaa
    24361 accgctttgc cacggtattt gccaaaggtg aaggttgtca tcaacgacgg acgtccggtg
    24421 atatcggcca tctgttctgc cgtccagccg gaggtgttca taatatcgat aagcaacgcg
    24481 gcggtgatat aacagtcata caacgcgcgg tgatgatgca ggcccggcgg tgtctgtaca
    24541 ttgagcttgc gtgttttata taacgccata ttgctgtact tgagccctgg ccacaaacga
    24601 cgggccagtt tcatagtgca aatccactca ccgggcatct caggcagtac gcggcggtca
    24661 aagctggcgt tgtgcgcgac ataccattca ctaccgtaat agtgtgggat cacatcttca
    24721 atccacggtt tatcggcgac catggcttcg gtgatgcgat gaatcgccat cgcttgtgga
    24781 ctaataggac gatcggggcg caccaggagg ctcatggggt tgacgatttt tccgtcaatg
    24841 acatcaacag aggcaatctc aacgatccct ccctgcaaac cgcaggtttc tgtatcgata
    24901 atgcgcaaca tggcatgctc caggccgaaa aagcctagcg taatggaatg atatcgcctt
    24961 gccaaccctg tgatgaacgc tgaccgacta acaataatga accacgatcg gcgcgaacga
    25021 tgagacgacc atattcatcc catagtgcac tgttaccacg ggcatttgcc atcagcacgg
    25081 caatcgagta ctgatgtgaa aaaaattgta agcgacgggc cgacgccagc aggtcgggtt
    25141 ctcccagaca ttgccctgtc gtaaacagtg aacaggtcgg gtccatatcc ataccttgcg
    25201 gttgttcatc gaccaccgtg atggttctgg aacgacggcc cagacatgca ccatgactct
    25261 gatggtagat cccaggcgtt tttcgccacg gtgcgaacac cgcaattcca ggaataaagc
    25321 gatcgttata ttcaacggga aggccggcaa tgatggtcat tcgccaggtc gttgcggcat
    25381 aacaaagcgg gtctaacagt gaaagatcgg gtggggcagg aagggcacgt cgcgaataat
    25441 cacaccccag taaagaaagc gatggaaaaa ctaacagctg acattgttgc ctggcggcgg
    25501 ccctcacaaa ctccagatga tgagctacct gttcggtgag tgaggtttta cgaggctcat
    25561 attgcgctgc tgcaactttc cagaacgaca taatgacttc cttttcaaaa tgactgcaaa
    25621 ttatgggggc ggtttagaaa atactcataa tttagcaggc gttatgtaag aaagtgtaac
    25681 tctgtataag ttttatttaa gtttgggctc gtaaggtaaa cgtgacagat tgaccgaagc
    25741 caaacggtgg gcaataagcc gctcgcgaaa ccagctgcgc aaatgttctg gctgttcacg
    25801 ttcaaccgct tcagcgatca ccggcatatt gtagcgttct ttaaaagcca ccccggccgc
    25861 cgccaaatcg acattcactt tatccatttc tgtttgatcc agtttagcca gattcttcag
    25921 cattcttaat ctcctcatca ttcgcgggca aaagttacta agagtgagcc tggatggcaa
    25981 gccggaattg atgatcctcg actcagacat tagacaaggt tatgctctgt aaaaaagtta
    26041 ttacaaaaag gaatagataa tatggcttca actgccgctc ccttcgctac gcgctggcaa
    26101 tcctgaccac ttcgttggtg acaccttccg tctgggcaca tgcgcattta acgcatcagt
    26161 atcctgcggc aaacgcgcaa gtgacagctg ccccgcaggc aatcacttta aacttctcgg
    26221 aaggcgttga aacaggattc agtggtgcaa aaatcacggg gccaaaaaac gaaaatatta
    26281 aaacattacc agcgaagcgg aatgagcagg atcaaaagca actgattgtt ccactggccg
    26341 attcgctgaa acccggaacc tacaccgttg actggcatgt tgtttcggtg gacgggcata
    26401 aaacgaaagg acactacacc tttagcgtga aataaatcat gctggcgttt acctggatcg
    26461 cgctgcgatt tatccatttc acctcgttga tgctggtctt tggctttgca atgtatggtg
    26521 cctggctggc ccccttaatg attcgtcgct tgctgacgaa gcgttctctg cgtttacaac
    26581 agcacgctgc cgtctggagt ttgatcagtg ccactgcaat gctcgccgtt cagggcgggt
    26641 tgatgggaac gggatggaca gatgtatttt cgccaaacat ttggcaggct gttttgcaaa
    26701 cgcagtttgg tggcgtctgg ttatggcaaa ttgttctcgc cctcgtaacg ttgatcgtcg
    26761 cccttatgca accgcggaat atgccacgtt tgctgtttat gctcactacc gcgcaattta
    26821 ttctgctggc gggagtgggg catgcgacgc tgaatgaagg ggtaacagcg aaaatccatc
    26881 agactaatca cgcgattcac ctgatttgtg cggcggcgtg gtttggggga ttactgccgg
    26941 tgctctggtg tatgcaactc atcaaaggcc gttggcgaca ccaggctatt caggcgctga
    27001 tgcgtttttc ctggtgcggg cattttgcgg tgatcggcgt actggcaagc ggcgtgctta
    27061 atgcattgtt gattaccgga tttcccccca cgcttacgac ttactggggc caactcttat
    27121 tgctcaaagc catcctggtg atgattatgg tggtcattgc gctggctaat cgttatgttc
    27181 ttgtaccgcg catgaggcag gatgaagatc gtgcggcacc ctggtttgtg tggatgacta
    27241 aactcgaatg ggcgataggt gcggtagtgc tggtgatcat cagcctgctt gcgactctcg
    27301 aacctttttg atggattacg aaaatgatga aaaaaagtat tctggcgttt ctgttactca
    27361 ccagttctgc agcggcgctg gcggctccac aggtgataac cgtgagtcgt tttgaagtgg
    27421 gtaaagacaa atgggcgttt aatcgcgaag aggtaatgct gacttgccga ccgggtaatg
    27481 ctttgtatgt catcaaccca agtaccctcg tgcagtatcc tttaaacgat atcgcacaaa
    27541 aggaagttgc cagtgggaag accaatgccc aacccatttc ggtgattcag attgatgatc
    27601 ctaacaatcc cggcgaaaaa atgagtctgg caccgtttat agaacgagct gaaaaactct
    27661 gttaattacc taaaatagcc ttttgatttc caataaaaaa accgcctcag ttctttcacc
    27721 agaacgggcg gtttttaaca tttaagctga tgaccaccgc gctttttatt gaccattttg
    27781 cacgcaaact ggaaaacctg gcgtcgtcat ctattcttaa agggcaaggc aactaagcct
    27841 gcattaatgc caacttttag cgcacggctc tctcccaaga gccatttccc tggaccgaat
    27901 acaggaatcg tattcggtct ttttttaatt gcatttgaaa tcaattagtt gcaaatgtct
    27961 cccccgaaat tccccgaaat ttactcgaat ttctgtattc cggtcttttt ggttatatca
    28021 caaccaaaat acatttaaca atccatttac gttaaaatca gagcagtaag tacgtttttt
    28081 ctctctcatc aagatacatt tttgttgtct tctccgatgt gtggccaagt agacgctgag
    28141 caaattcttc tccacatgtt tctttgtaca atcgtccagc caggcttctg atctcgtgaa
    28201 aagttggtgg gttttcactg aactggatac ctgtgaattt tctggctgcg acaaattttt
    28261 ttgtcaggcc atccgggtga atgctgccgt cagggctgtt ttttctaatc ccggcactga
    28321 ttagataatc tccccggctt accaggcggc atcgttcaac tactgtacca agccgtagac
    28381 cagcgacagg aaggctgagt gacaggggga tagcaatcat cattcctgtc ttaatttgcc
    28441 tgacgtggag acgatcatca taaatatcac tgaaccgcat attcgttatg tcttcgcgac
    28501 gttgtcctgt tacaagggct aaatccatag ctaatgggaa ccatgccgga agttgatctg
    28561 ctgcctccct gatgcagttg tatgtcttta gtttcagtcg ttctcttgta actactattt
    28621 tcggtgctct tgttggcgtg actggatttt gagatatacg tccttcaaca atggcctcgc
    28681 gaaacatatc agacaacaca gaacgcattg atcctgccat tgtgtttttc cctccttcaa
    28741 tccacaaatc aagaaactcg gcaatatggc gagtggttat ttctgtcagt aaaatctctc
    28801 ccaatttttc ttttattgtc tccagttgat ttgcccgaat tttataagta tttctggaca
    28861 cttttctcct tataagaatc gttttgtaac gttcaatcca gtctgccata gtaaatgagt
    28921 cgaacccttt aagtttttca attaaggcag cgggagagta gtttttgtat atataatgat
    28981 ttgcttcaat tgcctgcgtt actgcatctc ttcttgaaat tttacctagt gtaaattctt
    29041 ctttcgtcag agggttgcgc cagtaatatg ctttgtctct ccttcgatat gttaagtttc
    29101 taggcaaatt gggatcgtat tttttccgct gcatgtttta acttctccat taac
//
LOCUS       DADKXM010000040        28993 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.040, whole genome shotgun sequence.
ACCESSION   DADKXM010000040 DADKXM010000000
VERSION     DADKXM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.040"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..449)
                     /locus_tag="JQR05_001708"
     CDS             complement(<1..449)
                     /locus_tag="JQR05_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HAY5323898.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKK"
     gene            complement(480..1039)
                     /locus_tag="JQR05_001709"
                     /pseudo
     CDS             complement(480..1039)
                     /locus_tag="JQR05_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            complement(1051..1871)
                     /locus_tag="JQR05_001710"
                     /pseudo
     CDS             complement(1051..1871)
                     /locus_tag="JQR05_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1871..3220)
                     /locus_tag="JQR05_001711"
     CDS             complement(1871..3220)
                     /locus_tag="JQR05_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY5323899.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(3266..4024)
                     /locus_tag="JQR05_001712"
     CDS             complement(3266..4024)
                     /locus_tag="JQR05_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323900.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(4056..4769)
                     /locus_tag="JQR05_001713"
     CDS             complement(4056..4769)
                     /locus_tag="JQR05_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY5323901.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            4943..5635
                     /locus_tag="JQR05_001714"
     CDS             4943..5635
                     /locus_tag="JQR05_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY5323902.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNHVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(5684..7183)
                     /gene="pitB"
                     /locus_tag="JQR05_001715"
     CDS             complement(5684..7183)
                     /gene="pitB"
                     /locus_tag="JQR05_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY5323903.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(7475..9334)
                     /gene="gss"
                     /locus_tag="JQR05_001716"
     CDS             complement(7475..9334)
                     /gene="gss"
                     /locus_tag="JQR05_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY5323904.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            9539..10405
                     /gene="yghU"
                     /locus_tag="JQR05_001717"
     CDS             9539..10405
                     /gene="yghU"
                     /locus_tag="JQR05_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY5323905.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSCFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(10528..10776)
                     /gene="hybG"
                     /locus_tag="JQR05_001718"
     CDS             complement(10528..10776)
                     /gene="hybG"
                     /locus_tag="JQR05_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY5323906.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(10910..11251)
                     /gene="hypA"
                     /locus_tag="JQR05_001719"
     CDS             complement(10910..11251)
                     /gene="hypA"
                     /locus_tag="JQR05_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY5323907.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCRGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(11244..11732)
                     /gene="hybE"
                     /locus_tag="JQR05_001720"
     CDS             complement(11244..11732)
                     /gene="hybE"
                     /locus_tag="JQR05_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY5323908.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMYDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(11725..12219)
                     /locus_tag="JQR05_001721"
     CDS             complement(11725..12219)
                     /locus_tag="JQR05_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY5323909.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(12219..13922)
                     /gene="hybC"
                     /locus_tag="JQR05_001722"
     CDS             complement(12219..13922)
                     /gene="hybC"
                     /locus_tag="JQR05_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY5323910.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVVIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(13919..15097)
                     /gene="hybB"
                     /locus_tag="JQR05_001723"
     CDS             complement(13919..15097)
                     /gene="hybB"
                     /locus_tag="JQR05_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY5323911.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(15087..16073)
                     /gene="hybA"
                     /locus_tag="JQR05_001724"
     CDS             complement(15087..16073)
                     /gene="hybA"
                     /locus_tag="JQR05_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY5323912.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWLNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(16076..17194)
                     /gene="hybO"
                     /locus_tag="JQR05_001725"
     CDS             complement(16076..17194)
                     /gene="hybO"
                     /locus_tag="JQR05_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY5323913.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(17383..17670)
                     /locus_tag="JQR05_001726"
                     /pseudo
     CDS             complement(17383..17670)
                     /locus_tag="JQR05_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
     gene            complement(17789..18676)
                     /locus_tag="JQR05_001727"
     CDS             complement(17789..18676)
                     /locus_tag="JQR05_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY5323914.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMMA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            18833..19873
                     /gene="gpr"
                     /locus_tag="JQR05_001728"
     CDS             18833..19873
                     /gene="gpr"
                     /locus_tag="JQR05_001728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY5323915.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDKNTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(19913..20407)
                     /locus_tag="JQR05_001729"
     CDS             complement(19913..20407)
                     /locus_tag="JQR05_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY5323916.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            20598..21482
                     /gene="yghA"
                     /locus_tag="JQR05_001730"
     CDS             20598..21482
                     /gene="yghA"
                     /locus_tag="JQR05_001730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY5323917.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(21754..22179)
                     /gene="exbD"
                     /locus_tag="JQR05_001731"
     CDS             complement(21754..22179)
                     /gene="exbD"
                     /locus_tag="JQR05_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY5323918.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(22186..22920)
                     /locus_tag="JQR05_001732"
     CDS             complement(22186..22920)
                     /locus_tag="JQR05_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY5323919.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            23172..24359
                     /gene="metC"
                     /locus_tag="JQR05_001733"
     CDS             23172..24359
                     /gene="metC"
                     /locus_tag="JQR05_001733"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY5323920.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLYIGLEDVDDLIADLDAGFA
                     RIV"
     gene            24499..25158
                     /locus_tag="JQR05_001734"
     CDS             24499..25158
                     /locus_tag="JQR05_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5323921.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(25198..26097)
                     /locus_tag="JQR05_001735"
     CDS             complement(25198..26097)
                     /locus_tag="JQR05_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5323922.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            26291..27454
                     /gene="yqhD"
                     /locus_tag="JQR05_001736"
     CDS             26291..27454
                     /gene="yqhD"
                     /locus_tag="JQR05_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY5323923.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHFSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            27559..28386
                     /gene="dkgA"
                     /locus_tag="JQR05_001737"
     CDS             27559..28386
                     /gene="dkgA"
                     /locus_tag="JQR05_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY5323924.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPETDQFGG"
     gene            28586..>28993
                     /locus_tag="JQR05_001738"
     CDS             28586..>28993
                     /locus_tag="JQR05_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY5323925.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWV"
BASE COUNT         7327 a         7706 c         7132 g         6828 t
ORIGIN      
        1 aatttctttg cccgcaactg gcgaacatcc agctccgact gcgactgctg ctccagttga
       61 taaatctgcg cataagccca cggacgacca tacatcgaca gaagggtgac aaaaatactt
      121 agcggtatcg ccagataaag aacggctttg tacaagcgtc ccggactgcc ccccgccgca
      181 gagatagcgg taatttccga gtcggtatac atctgcccta gcgtcacgcc aactgacaca
      241 tacagaccaa caggtaacaa catctccagt gcaatcagta ctttgtagaa aacgatatcc
      301 agcacaacat caagagctaa tgtcccattt gccgcttcgg tcagataacg ctgtgcggag
      361 tagctggcga aaataaagat cagaaaaccg acgataatca gcaccagacg gcgcgtacca
      421 cgcatgatgt agtgttcaac cagctttatc tcactaacgc cctcaatcct ttgagttttt
      481 tattcattga tcagtttctg cgccaacgca atgacatgat ctacctcttc cggcgtcagg
      541 ctgccgtcct ttgcccactg aatttgcccg gctttattca gcaccagaat ggttgaactc
      601 tgctccggca agcgccaggc aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc
      661 cacggatagc gcgtttattt ttttcgattt tgccaacaac aaaatagcca gtaccaaaga
      721 tggcatcatc ggtgttcaca atggtggtgg gctgaaaacg atcctgcgga aagtttgccg
      781 cttcaaccgc tttaattaac agtgagttct ttttcttcgc cgacttacgc ccggcgatat
      841 actgaataat gcggactttt cccgctaatt gtgtgctgtt ccacttttgg tagtgaattt
      901 cgtcgtcgct attcagaagc agctcgccgc cttcctggat ataaacaggg gtaaccgttt
      961 tgccagtgac gaaattgtgt gcctgcacac ccggggctaa gagcatgata atcaacgcaa
     1021 taatcaaacg agatgacatc attgctccgg ctatagcgga aaacgcgaaa tcagcagctc
     1081 taagcgctga cgaatatcgc tatccagcgc caacggtgca tcatacgact ggcggttttc
     1141 catatcatcc gccaccatgt cgtcgtgttt atctcccatc aggccattaa tggcggcgag
     1201 gaaacgggaa atctgtaacg cctctttatt ttcaggatca agcgatctta aggtgtcgta
     1261 ataagaaacc tgctggctga tgatttttcg cacatcatga aattccctgc cggttagtcg
     1321 cggtttggca ataatctctt tcagggtatg catttgcttt tgccgatagg caataaaact
     1381 gcttacgctt tctaactggc tatggcgtaa tgaatagttc accaacgacc agaccggaga
     1441 gcttaaataa atccgtaaca ggttgccgta atacgacaca atatttttct taccgttacg
     1501 aaagccgtcc tgaaaacgcc ccagaaaaac ggtcgttttg ccgaataaaa atccggcctg
     1561 atgcttcttt aaatacagcc gttgcgcaaa acgcaggtgc ttgtagcgcg ctcgcatgta
     1621 tttaaattgt ttgcgctcat cagcagtcag ctcatcgcct ttggcttgtt tgcgaactaa
     1681 acgcagtaat tcctgacgat caacgccatt gagccagaat tgcaggcata acgcgtaatt
     1741 atcatgaatg atttcggtgg tacaacttag tgcaatagtc ggcggcaaac taacctgtgc
     1801 atcaacgaca tcatcttctt caatcgcaac aaatagacgc tcgcctcaag cgggaaatat
     1861 tttctcatca tcaggatttc tctccggctg cgttataaag caaggaatgg cgctgaaaaa
     1921 atgcctttaa ggttggaatt aacgagagcg ccacgtccag acattgccca ccgaagtaag
     1981 caaacgctgc acccagcaca ccaaacagat gacagaacat gaacagcaat acgatgtagc
     2041 cgattgaggc gatggtttgc gccacgagaa aagcgcgctg ttttcccgcc atcaatagca
     2101 atgactcctg cgggaagccc agcatcgaaa tcacaattgc gcccagcatc acctgaatca
     2161 ggtcatacgc ttcgagatac ttaacgccaa acaccagcga aatgagcggc ttgccgacga
     2221 tcaacaccgc gagcgccacc agaataccga ttccgcccgc cagtaaaccg gacttcacac
     2281 ctaacaacca cggtctggtg gtgcgcggat ctaaacgcat cacctctggg taaaaacttt
     2341 tacccagcaa acccgctggc gttccggcgg cgtcgaagaa tgtcatggcg attttaaata
     2401 acccggcggc agcgggtcct aacacgatcc ccaccaacac tgtgctgcac gagttacgcg
     2461 ccgaccagat ggagtgggca atgttggttg accagacaaa actccacgcg cctttaatgt
     2521 gtcgggcaga ctcaaacaga ttcaatttga aggcgttatg gatatttcta cggcgtaatt
     2581 cgcgcgcggc aaaccaccag tacatggtgc cgccaaccag attcgacacg taccaggcaa
     2641 taacaaaacc cgcaaaacca aagtcaaaat accaggctgc gacgctcccc gctgcgcgca
     2701 gaaaaggttt cgtcgcctgc tgtacagcaa tcaaatcaaa gcgatctacc gcacgcagaa
     2761 tgccggtcgg cgtggaggaa gccattgaag gaatgagcgt gcaatacagc gccgccagcc
     2821 aaaagctttg gtcatccaga cctaatgaat gggaaaggaa tggcagtaag gcaatgccgc
     2881 cgacaatcgc caccgcgccg ctaacgatat ccagcgagaa ggaaaatgag acgacattgc
     2941 gaaattgctg cggattattg ttggtcaagg ctggcgttcc gtactgaacc accagctgcc
     3001 atgtctgaaa cttaataaaa tcgctgatcg acttggcgta cgattgcaca atcaccagta
     3061 cgccaaacat ggcgggcgtc atccctttac cggcacacga gagcgccaac agccccagca
     3121 aggcgctctc gacattactg gagcctaacc aggcgctatt gcgaataatg gtgcgaaacg
     3181 cgccatctgc aaaccaatgt ttgatgttaa aacccgccaa ttcagcctga ccttatcgtt
     3241 gatagtaaaa aatatcccgc cagccttaag ttaaacttcg gcggtcagaa acgatggcaa
     3301 ccagagaaac cgccttctgt gcctgttcca gcacttcgct gtagggcgct ctggaatcaa
     3361 tctcaagaat ttttgtgccg ttatagccaa tcgtcgacat gacgccgatt ttgtcctgca
     3421 gctcggcata ctcatggtca ggcttgcggg agatggcagt atcaatatca atccccaaac
     3481 gaatgattaa ttcaggacgg tattgcgcca ttttttggta taaacgccgt tcgcgctgcg
     3541 ccagaaacat gctgattttc ccggtcgcac gttcgacgcc gatccccggt ccgtcataat
     3601 aaaagcccga aatttcagcc tgcgggaagc gatcgctgac caccagaacg ccactttgcg
     3661 ccagtcgctg aaccttgcgt agattcgcca ttcttcgcag cgagaagcag tacataatca
     3721 ccgccgccca tagcgccgga gatttggttt tcatgctttg ggttttcgag gatttggccg
     3781 ccagtcgccg ttccagccag acgccaacca acggcaatcg tttgattttg tcgccgtctt
     3841 cgccggagag cagccccaga tagcggcgct cggtttgcca gtgttgttgc agcgatttca
     3901 ccaggtcggt ggtcagcgtg gatttaccgg tgccatcaca cccgaccacc gcaatcagcc
     3961 ccggaatgta gttgggctgc ggcgcggttg tactattaac agtttgtgtt tgtagtgcat
     4021 ccattcgtta aatcatcccc tatccatttt ggtgatcagg aaagcgcggc gtgaattgcg
     4081 cgtagtgacg cttgcataat ttcatcggct ggttgccgac catccagttc aaggatcttg
     4141 gcgccattga atgtcagttg cggcgtaacg gcgatttttt cctgtaacgc tgccagttgg
     4201 tggtcaggtt tacgcgcaaa cgcggtttgt tcatcaatgc caagacgaat caacaatacg
     4261 ggtaaataag acgccatcca ttggtacagc ttcaactcgc gctgccttaa catttttatc
     4321 caaccgttac cgcccgtggt ttttgccaat tgcgggccat caaagcgaaa acccggcact
     4381 tcaacctgcg ggtagcggtc ggtgatgagc agatagcctt gctggctttt acacaacatt
     4441 ttgcgaaact tgtacgcccg ccagcaggaa agcagataga taaccagcgc agtaatattg
     4501 ccaggcggtg ttgagggctt ttcgtgtaca tgtgccgctt tacttcgcag ataacaccca
     4561 caaggtgcgc caataacagg gagctgtgaa atccattcgc caattcgccc ggacgattgc
     4621 ccgagataaa tgtgttctgt tggcattctt gctgccagtt catttaccag gcttgccgtg
     4681 agggtcgatt tacctgaacc atcacatcct acaatggcaa taacacgcac tggcgtggaa
     4741 ttaattattg acatattacg ttgattcacg aaaaacccgg cagtaaataa tgtattgaat
     4801 attacgtggt ccgtgctcag aatatccgtt caaccttatt gtgacaatgg ttaattgtga
     4861 cattgacctg aattacgctt tcataaaaac atattaacca aataaatatt tttaatggat
     4921 atttaaatta aaggatatat tcatgcagtc aataacacct ccattaattg ccgttattgg
     4981 tagcgatggt tcaggcaagt caacggtgtg tgaacatctt attaccgttg tcgaaaaata
     5041 tggtgctgcc gaaagagttc atttaggaaa acaggctgga aatgtcggtc gtgcagtgac
     5101 aaaattacct ttgatgggaa aatccttaca taaaacaatt gaacgaaatc atgtgaaaac
     5161 agcaaaaaaa ttgcctagac cagttccggc gctggtaatt acagcgtttg tcgcccgtcg
     5221 cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga
     5281 ccgttatcct caggaccaaa ttcctggcgc ttacgatggt acggtgttcc cacctaacgt
     5341 tgaaggtggt cgttttgtct catggctggc aagccaggaa cgtaaagcgt ttcactggat
     5401 ggcgagccat aagcctgatc tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc
     5461 acgtaaaccc gaccataaac gggaatcgct ggcgaggaag attgccataa cgccacagtt
     5521 aacctttggt ggtgcacaac tggttgatat cgatgccaat cagccactgg aacaggtgtt
     5581 ggttgatgca gaaaaagcga ttacggattt tatgaccgcg cgtggttatc actagtcaaa
     5641 aatggaaatg cccgatcgcc aggaccgggc attttcagga aggttaaatc aactgcaatg
     5701 ctatccagta cagcccacca gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca
     5761 tcaggatgct ggttaccgtt ttacgctgta acccaccgcc gtccaccacc atcgtccctg
     5821 ctaccgcaga cgagaggacg tgtgttgtgg agacaggcat cccaatataa ctggcaaggc
     5881 cgatagacac tgccgccgtc atttgtgccg ccatgccttg cgcatacgtc atgccgcgct
     5941 taccaatctt ctcaccgatg gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc
     6001 cgagcgccaa tgctaccgcc atgatgatcc acaccggagc gtactcaatg gtgcttaaca
     6061 tatcgctgcg cagttttttc agcaggttct ggtcttcttt actgacgcct ggcagtttcg
     6121 ccagcttcgc ggaggtatca gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac
     6181 tcacgcttaa cggctcgtaa ctttccatat tgcctggcag catcgtttta acgcgcgcaa
     6241 tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg
     6301 atgctgcagg caatggaggt tccatcgcaa tcaacttctg cggcagttca ggatgctgtt
     6361 gcaggtagtg ttcgaagttg gtaacggcat cgcgggtacg ggtaatttca tagccggacg
     6421 cattcatatt gacgacgaag ccagcagggg caatccccac cagtaccagc attaccaggc
     6481 cgatcccttt ctgcccgtcg ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa
     6541 tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc ctttttcttt ttgcgatctt
     6601 ccggaatgcg gtgaatacgg tcacgctttt tcgtcccact ccagaaacgg cgcagcagga
     6661 aaatcagacc acccgcaatg accaggccga cgatagggga aacaatcagc gaggagaaaa
     6721 ttttggttac ttcacgcagg tttaacgcat ccatcaccga tgagccggtt aacagcgcgt
     6781 tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac
     6841 caaagaacca cgttcccagg ttccagataa tcgccgccag cagcatggaa aagaccatcg
     6901 ccagaccgtg agttgacccc atattcagca acaaatcggt tggtaacata tggacaatgg
     6961 cataggcaac gctaagtccg cccaataaca cgccaaaaaa gttaaaaaat gccgccatca
     7021 ccacagcaag ttgtggctgc atggcacgag tataaataac ggttgccacc gcattcgccg
     7081 tgtcatgaaa accattgatt gcttcgtaga acaacacaaa tgccagagca agtaataaca
     7141 aaagccccgt atatatatca aggccaacaa ataaatttag catatattag attacgccat
     7201 tttgaattta cgaacggacg cattatcagt gactttaacg gcatgggcaa agtgaaatat
     7261 cattttttaa cgataaaaag agtgttattt cttgtgtatc agacaataag cattcattca
     7321 aaacctttag aaataatcaa aacacaggta ttccactggt taatatattc acatatgaaa
     7381 tgaataaata ttggaatata taaatattga atattttgat taatccccgt actaattatt
     7441 cttcataatc agtacggggt tgctacaaca tgtattactt tttcaccaca attaacggtt
     7501 caatatcact ctcttttttg atgaccagtg attcatcacc gcgcaaacac gtcccaccgt
     7561 agttgccgcc aacggtgaag gtacatacct gaatgtattt accgtccact ttcggcaaac
     7621 accacagttg ctgatagatg tttttctgct cggcaaattt accgctggtt ttgtccagca
     7681 cctcttcatg atggctgacc aggtcgatat tgctaccaca gcgaccggcg atcggtttca
     7741 ctgcgtaacc tgttttcacc aattcatcat taacagtgaa atcggtatcc agcaggtaac
     7801 gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc tttgttgccg gggatcaccg
     7861 tccacagcgg ctcaaagacc agcacttccg ggcgcagcaa tacgtcgata agacgcactt
     7921 cgttttgcgg atgaccggta cggattggca ccgcagcaaa ctcacggtcg ctaacttcac
     7981 gaatctgatc aaacgcggtt tcccacgccc aggttttcca cacgcagtta accagtcgcc
     8041 cttccccatc aatcagttgc ccggcagcat cccagcccag ttcatccagc ccgcgcaaga
     8101 tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat aaactgcgcg tgatagtttt
     8161 cctcgatatc tttgtcctgc atgatatgga caaacggacg tgcacgactg tgtttccagg
     8221 caccagccag ttcgttaatc agcccttccg ccggattgaa gccgttgcct ttatagccct
     8281 gctccgccca acgttcgagg atcaagcccg cttcggtatg acaggaggcg gagtcggcgt
     8341 tgtattcgta aaccttcagg ccacgctcat ccatgcagaa atccatacga ccagtgatca
     8401 tatggtgacg gcgacgctgc caggagagac gcaaacgtgg ccagaggatt ttcggaatgt
     8461 cgaacagcgc cagcaggttg tcatctttca gcaccttgtc ggttgcgtga agatacatca
     8521 gatgcagctc gttggtggct ttaattagct cctgctcggc actctcggta atggtgtagt
     8581 aatgatacgg atcctgattg atcacctgac cgttggcctg cacataggcg ttttgtagcg
     8641 gatctttttc atccagccat ttaccgtcaa actggccttt gttttccaga cgcgctccac
     8701 tgattttcag cagctcgcct gcaatttccg gctgcggtaa gctgtattca gtatcttccg
     8761 tctggatcat ccagcccaga atggtggtgt catcaaaagt gtctttaagg gtatagcagc
     8821 cgttttcgac caccatctcc agctcgcgcg tccactgttg cccttgcggc aacggggaat
     8881 gaatcacgtt ctgttccgca atacggactt tgttgccatg caattgggta atgatggcga
     8941 catggccagt gtctttaaat tcaccgcctt tatcccagat aagaagcgca cccgcgaccg
     9001 gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag gatgttgtca ttaaccactt
     9061 ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc cacgtcagta aagaccacac
     9121 cgtaattcag aaagagaaaa cggcgagcaa attcaacgca ttgccacttg tggcccatat
     9181 attcgtcgtc gatatagcta cggaatacgg cgtcatcttc gtattcctgc ggatcgagag
     9241 aactgtaatc tgaagagtag attgctaccc cacctggggc gtagcccaat aatgtcccga
     9301 acggggcatc ctggctggtc gttcctttgc tcatcacttt acctttaaca atacaaccta
     9361 agcagttggc gtaattgttg tgctctgatt acctgctcag cgacattaac cggacagagg
     9421 tcagactaat catacacctc atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc
     9481 tgtacagcaa gtgacctgct acactgcttc aacactacca ctcagaaggc aactcactat
     9541 gacagacaat acttatcagc ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc
     9601 gttcgccaat atcaatcgcc cgttttctgg tccgacgcat gaaaaaacgc tgcccgttgg
     9661 caaacatcca ttgcaacttt attcgctggg aacgccgaac ggtcagaaag taacgattat
     9721 gcttgaggag ctactggcgc tgggcgttac tggtgcagag tacgacgcct ggctgattcg
     9781 tattggcgat ggcgatcaat tctccagctg ctttgtcgaa gtgaacccaa actcgaagat
     9841 cccggcgctg cgcgatcata cgcataatcc gccgatccgc gtgtttgaat ctggttcgat
     9901 cctgctttat ctggcggaga aatttggcta cttcctgccg caggatctgg caaaacggac
     9961 tgaaacgttg aactggctgt tctggttaca gggtgccgcg ccgttcctcg gcggtggttt
    10021 tggtcacttt taccattacg cgccggtgaa aattgagtac gccatcaacc gctttaccat
    10081 ggaagccaag cgcctgctcg acgtgctgga taagcaactg gcgcagcata agtttgttgc
    10141 gggcgatgag tacaccattg cggatatggc gatttggccg tggtttggca acgtggtgct
    10201 cggcggcgtg tatgatgccg cagagtttct ggatgcggga agttataaac atgtgcaacg
    10261 ctgggcgaaa gaagtgggcg aacgtccggc agtgaaacgt gggcgtattg ttaaccgcac
    10321 caacgggccg ctgaacgagc agttgcatga gcgccatgac gccagtgatt ttgagacaaa
    10381 tacagaagat aagcgtcagg ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg
    10441 gataaggcgt tcacgccgca tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg
    10501 tcttatcagg cctacaaaag ctaatcatca cgcgctggta atgtcgtaat ccatttggcg
    10561 cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg caaatccgac
    10621 gtgtaccagc acccactggc ccagcagatc ggcagggtta ccttcgcaaa tcagggcgat
    10681 attcacgtcg cgcttgatac cacatacttc aacctgcgca agctggtgaa tatcttcacc
    10741 gacagccagc acctggcctg gaacgccaat acacattgtt aactccggtt atttcgattg
    10801 ccggacaaac gcgaaaaaga acctttagcg ggtgttccct ctcaccctaa ccctctcccc
    10861 ggaggggcga ggggaccgtc cgtgcgcttt ttaggcgttg tcctcagtct tattcaactt
    10921 caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg ccgtgacaga
    10981 gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc cagcaccagg
    11041 cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt ccgcggcaga
    11101 caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg ccaatttcca
    11161 gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg ataatttcaa
    11221 cggcgctctg gcaaagcgac aactcatgca ttttcgcccc tcctgcggcc aaacagcagg
    11281 gcgcgacgcc ctgcgtgtgg tacatccgga ttggtgactg gcaacgaaag gatcattcgt
    11341 gcgcagtcat ccgtcaggcg ttggccttct tcaatcgaca tgctgtgcga aagcggcgac
    11401 atcagcgaac aggagaggta ttgcgaaaca ccgtccagtt cgccaacggt aaaggtcatg
    11461 gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg ccagagttga
    11521 tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca ccccgtccac
    11581 tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttgaaggatg cagaaaagag
    11641 agatcgtaca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg cgccttcggg
    11701 gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga tggcttccac
    11761 gcctgattcg cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca ttgcttcaac
    11821 cgtcggcgtt aagccgatgt gtggctccag cgattccggg atcacgccga ccagggtcag
    11881 ctttttcgga aactcaccgg tgaagcgcag ggccgacaat acgtcggcca ggccaagctg
    11941 atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca ggatcatcat
    12001 cgttcccggc gtgttctttt tcgacacaat ggcatccgca ataatcagat gatcgcgatt
    12061 tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct caacataatc
    12121 cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acgccgatgg cttcatcggt
    12181 cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt cactgaaacc
    12241 acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg gtcgaaggag
    12301 tgaatggtac gcaccacttc cagcggttta ttcggatcgg caaccggtgt acccaccagc
    12361 gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt ccaagttgat
    12421 ggaacaaccg cctggtagtt gctgatgata ccgtctttaa taaccatcca gtgagagagc
    12481 ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc cggaatgttc
    12541 ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc actgtattgg
    12601 ttttgcagga tatcctgcaa ttcacagcag tgaacggtac gaccaataat acggcccagc
    12661 gtggagtgca gctgtgccac ttccagcgtg ttgccagtca gtttctgata aatcacaacg
    12721 atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac cagcatatta
    12781 gccagcggcc ccacttctac cgttttgccg tagaaagtcg gtgatttcac ccaagaatat
    12841 ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca cggcgcctgt
    12901 ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt gatcaggtat
    12961 tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc aatgtagccg
    13021 cccgggaaca ggaagctgcc gtttttgctg tcggtcggga attccggcac gctcaggtag
    13081 ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcgat aaccgcggta
    13141 tcaaccttat aaacctgctc aacaaagtcg ctcagtttgt cgatgaaaga cttgatgtac
    13201 atcaggcgct caaggttcaa cacgcccaac ccgtcgaggt tgattgggtt cgcgacacca
    13261 cccactgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac gacgcggtta
    13321 gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag gttcacttcc
    13381 ggcggcagtt tcatcgccgg gtgaccccag tagccattgg cgaaaatacc caactgaccg
    13441 ctggcaacca gatctttgat cttgttctga actttagtga actcttccgg actgttcaga
    13501 tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc ttgcagtgca
    13561 gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat atggtcatgc
    13621 gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg aacgtcgata
    13681 ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt acatacgcca
    13741 cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac gatctcttcc
    13801 atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt ttcgatttcg
    13861 caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat tctctggctc
    13921 atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga agtatcggca
    13981 gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca atagaaatca
    14041 acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat gccaccaacg
    14101 aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac aggaacagca
    14161 tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag agcatcagca
    14221 ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac gacagcttgt
    14281 cgcgatagat aagctcgcca aagcgcagcc cgatgaaaat cgccagcaac acactgatgg
    14341 tgttggtcag cttgacgaac aggctctttt catccggacc gttgccacgc agacccgcct
    14401 gcaccagtga accttcaaag atgacaatcg agaagcccat gatgaacgcc gtcagcagcg
    14461 agaacagcgg caacatttca tagctctgcc agagcggatg caccttgtag cccgccgaga
    14521 tcatcagcga ccccattgaa gactggtgca tggtcggcag cagcgcaccg agcgcgataa
    14581 tgaagaacat caccttgttc agacgttgta gcgacacctt ccagcccaga cgttcaaaca
    14641 gtgccggagc aaactccaga gccatcacgc cgatgtagat ggtcatacag accgccgtct
    14701 cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc aggttccagt
    14761 agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac agactcgcca
    14821 acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat accgcccacg
    14881 ccagcgccca gccgccacag gcaaagccgg tgccaatcag caggtcaaac gcgatccaca
    14941 cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac accagacgct
    15001 tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc ggtttactga
    15061 tgattttgcc gcccagcggt tgtggatcat gactcatgat cgtctcctcc gtcgtgatgg
    15121 tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc cagtggtagc
    15181 atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt agaaagatcg
    15241 tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag aacctgagta
    15301 ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt atgcaggtaa
    15361 gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg cttcagcgcc
    15421 agacgttttt tcgcctccgc catcagctct tcacgcgtac cgaaaatcac cgcgcccgcc
    15481 gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac acctttctgg
    15541 ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta cttcggcacg
    15601 ttgtacggac aggcgaccat gcagtaacgg caaccggtgc acacgtcttt gtcgtaatgg
    15661 acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga gacacagttc
    15721 ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc ctcctggtct
    15781 ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata cggcgacagt
    15841 ttgtcgttgt tcaaccaggt ctgttcccct tgcgggttac gttcagggaa gttgatatcc
    15901 tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta caacatcccc
    15961 agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga cggcagcgcg
    16021 cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt cacggttatt
    16081 ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc accgccatca
    16141 cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa accaatagcg cctgcagaga
    16201 cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc tgattttcga
    16261 cgttggcaag ctgatggatg cctttatgga agccgatacc tttttcgtta cagccatagc
    16321 acgggtgacc aatcgccacc ggccacacac cgccaacatc gcagaattgc agcgttgagc
    16381 agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag ccttcgcggt
    16441 ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg cgacgttcgc
    16501 agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca tccagtttcg
    16561 gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc gggttcggcg
    16621 ggcagcccgg aatattgata acggttttgc ctggcagaac ttcttgcaag ctgactgcgc
    16681 cagttgggtt aactccagct gcggcaacac cgccccacgc ggagcaggaa ccaatagcga
    16741 taatggctgc tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc tcaccggcaa
    16801 ccatgcaata aataccgtta tcttttaatg ggatggaacc atccaccact aacacatact
    16861 gccctttgta cttctcgaga gcgttatgtt tgttctcttc gacctgatga ccgaaggcgg
    16921 cggaaagcac ttcgtgatat tccagagaga tagtctccag cacgaggttt tctaccgttg
    16981 gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg ccaatccaga
    17041 taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg gctttgctac
    17101 ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga cggttaatgc
    17161 cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt gcgaagacct
    17221 ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca tttaccacac
    17281 atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga agggaagaag
    17341 cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt cgaggcgata
    17401 accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga aatccattac
    17461 catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg cgctaagcta
    17521 gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac cgcgtttata
    17581 atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt ttaatcccgc
    17641 cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgttata tatcgccata
    17701 tataacgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca tacttgttta
    17761 agacgtaatc ccgtaagaga taaacctatc aggagagata tttatcgaac catttcagtg
    17821 tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa tcattatgga
    17881 atccgtgatt aacccccgga tagatataag cctcataaac cttattattg tctttcaacg
    17941 ccgcctcgta aggaggccag ccctcgttga ttcgggtgtc cagttccgcg aagtggagta
    18001 gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca taaaacggca
    18061 ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca ccgccatagc
    18121 aaaatccggt aatacccact ttacctgtcg cttgcggata gcgttgcata aactcaattg
    18181 cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc tcacgacctt
    18241 tatcatcatt tcccggataa cctccaacgg aacttaatcc gtcaggtgcc agggcgatat
    18301 accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt ccacgattct
    18361 catgcaccac caccacggct ggcgttttgc cgctcatttt tgcgggtttc accagataac
    18421 cccgtacctc gccgtgacca tttggcgaag gatacgtgat gtactcagca acaatctccg
    18481 ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa tcaaacaacg
    18541 ctaatgccat catcccgccc accgcatact tcgccgcaag gttgaggaac tcacgtttcg
    18601 aaattttccc gtgagcgtaa tagtcgtagt aatccaacaa ctcttgtggg aaatctttgg
    18661 cggttaaacg cggcatcgtt acattcctca actggtgttt ttttaagcaa agcataagcg
    18721 cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac gcgctggcga
    18781 atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg tcatggtctg
    18841 gttagcgaat cccgaacgtt acgggcagat gcagtaccgc tattgcggaa aaagtggttt
    18901 acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta acgcgctgga
    18961 atcacagcgt gcaatcctgc gtaaagcgtt tgatttaggc attacgcact ttgatttagc
    19021 caacaattac gggccgcctc caggaagcgc agaagagaac tttggtcgcc tgctgcgcga
    19081 ggattttgcc gcttatcgcg atgaactgat tatctctacc aaggctggct acgatatgtg
    19141 gcccggcccg tacggctctg gcggttcacg taaatacctg ctcgccagcc tcgaccaaag
    19201 cctgaagcgt atggggcttg agtatgtcga tatcttttac tctcatcgcg tcgataaaaa
    19261 tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg gtaaggcgct
    19321 gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg agttgctgca
    19381 cgagtggaaa attccgcttt taattcatca accttcgtac aatttactga accgctgggt
    19441 ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgta ttgcctttac
    19501 tcctctggct cagggattgc tgaccggaaa atatctcaac ggcattccgc aagattcacg
    19561 gatgcatcgt gaagggaata aagttcgtgg tctgacgccg aaaatgctta ccgaagccaa
    19621 cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag cgtggacaat caatggcgca
    19681 aatggcgtta agctggttgc tgaaagatga tcgcgtgacg tcggtattga ttggtgccag
    19741 ccgcgcggag caactagagg agaacgtgca ggcgctgaat aatctgacat ttagcaccga
    19801 ggagctggcg cagattgatc agcatatcgc cgatggcgag ctgaatctgt ggcaggcgtc
    19861 gtccgataaa tgacctgtta ataacgggcg cgcgaaccgc gcccgaatca gatcagtgat
    19921 tatgacgagt cagtcggtca agatagccca tcacaaatgc agaaagcaca aacgtcagat
    19981 ggataatgac gtaccacatc agtttgttat ccgggacatt tttcgcatcc ataaagacgc
    20041 gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt ttcagcgacg
    20101 ttgcgtccat tttccccagc cagttcagct tctctttgtt ctcggagata tccagctgtg
    20161 agacgaaatt ctcataaccg gaaaacatca ccatcaccag caaaccgcca accagcgtca
    20221 tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag aagatattcg
    20281 gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac gccagcgaaa
    20341 ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt tcaagaaaac
    20401 gttccataca ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa ttgagtaaac
    20461 agcacgcaac agcaaatcaa catatacgtt aaacctttgt cgcataattc tgaggtttag
    20521 ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag gacgttgaac
    20581 ctgaagggag aaaaacgatg tctcatttaa aagacccgac cacgcagtat tacactggtg
    20641 aatatcccaa acagaaacaa ccgacgccag gcatccaggc gaagatgaca ccgataccgg
    20701 attgcggcga gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa gcactggtga
    20761 cagggggcga ttccggaata ggtcgcgctg ccgccatcgc ttacgcgcgt gaaggggctg
    20821 acgtggcgat cagttatctt cccgtggaag aagaagacgc tcaggatgtg aaaaagatca
    20881 ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga tttaagcgat gagaaattcg
    20941 cccgttcgct ggttcacgaa gcgcacaagg cgttaggcgg gctggatatt atggcgctgg
    21001 tcgccgggaa acaggttgcc attccggata ttgcagacct caccagcgaa cagtttcaaa
    21061 agacctttgc cattaacgtt ttcgcgctgt tctggctaac ccaggaagcg atccccctgc
    21121 taccgaaagg tgcaagtatc atcaccactt cgtcaatcca ggcataccag ccaagtccgc
    21181 atttactgga ctatgcggct acgaaggcgg cgattctgaa ctacagccgt ggcctggcaa
    21241 aacaggtcgc ggagaaaggt attcgggtga atattgtcgc gccaggccct atctggacgg
    21301 cactgcaaat ttccggcgga caaacgcagg ataagatccc gcagtttggt cagaaaacgc
    21361 ctatgaagcg tgcggggcaa ccggcggaac tggcccctgt atatgtttat ctggcaagtc
    21421 aggagtcgag ctacgtcacc gcagaagtgc acggcgtgtg cggcggcgag catttaggtt
    21481 aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattg tgaattcact cttacctgat
    21541 gtggcgaaaa cgccttatcc gcctacaaac gcatgaagat tcaacatgtt gcaatagatt
    21601 ttgtaggcct gataagcgta gcgcatcagg cattgagcac ctaatgccgg atgcatcgtg
    21661 aacgccttat ccggcctaca aacgcacgaa gattcaacat gttgcaatag attttgtagg
    21721 cctgatgagc gtagcgcatc aggcaattct actttacttc gctttggcgg tttcttcgcc
    21781 gaccagacct atcttcaggt aacccgcctg atgcagcgta tccattacct tcatcaacgt
    21841 ctcgtaatcg acggttttgt ccgctcggaa gaagatggtg gtgtctttct tgccttcggt
    21901 taacgcattc aacgccgtaa tcattgtttc atcggtgacc ggatcgttac cgataaacat
    21961 cgagttgtct gccttcaccg acagataaac cggtttttcc gggcgcggct gcggcgtgct
    22021 ggtagaagca ggcaagttca ccttcacatc taccgtcgct aacggtgccg ccaccataaa
    22081 gataatcagc agaaccaaca tcacgtcgat aaacggcgtc acgttgatat catgcatttc
    22141 gccgttatcg tcgaggtttt cgttaagatg cattgccatc ggacattatc ctgcgcgtaa
    22201 tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct tccagatcca ggtcacggct
    22261 ttgcagcaac aatacctgcg ctgcaacatc acccagcatc gctttaaagc cgccaatctg
    22321 gcgtgcaaat acgttataga taacgaccgc aggaatcgct gccacgaggc cgattgccgt
    22381 tgctaacaga gcttctgcga tacccggcgc aacgactgcc aggttagtgg tctgcgtttg
    22441 cgcgatacca ataaagctgt tcatgatgcc ccagaccgta ccaaacagac caacgaacgg
    22501 agaaatcgcg ccgatggttg ccagatagcc gttaccgcgg cccatttgac gacccactgc
    22561 ggcgacccga cgctccagac ggaagctggt acgttcttta ataccttcgt tatcgtcgct
    22621 gccttctgac agttccagct cattctgcgc ttcattgagc aaatgcaggc ttaagctttt
    22681 gctaccaaaa tcagcggcga tatcgttggc ctggtttaag gaacgtgctt cagccagcag
    22741 ttgctgctcg cgcttaaggc gacgcttctg attgaagaac tctacgctct tactgaagaa
    22801 gattgcccaa gtgactacgg aggccaaaat aagcccaatc atcacgcact taacgacaat
    22861 atcggcgtgc tgatacatac cccagacgga aaggtccgtc tgcattaaat tattacccac
    22921 tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa taatatcaaa acgacgtcga
    22981 attgatagtc gttctcatta ctatttgcat actgccgtac ctttgctttc ttttccttgc
    23041 gtttacgcag taaaaaagtc accagcacgc catttgcgaa aattttctgc tttatgccaa
    23101 ttcttcagga tgcgcccgcg aatattcatg ctagtttaga catccagacg tataaaaaca
    23161 ggaatcccga catggcggac aaaaagcttg atactcaact ggtgaatgca ggacgcagca
    23221 aaaaatacac tcttggcgcg gtaaatagcg tgattcagcg cgcttcttcg ctggtatttg
    23281 agagtatgga agccaaaaag cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg
    23341 gacggcgcgg aacgttaacc catttttcct tacaacaagc gatgtgtgaa ctggaaggtg
    23401 gcgcaggctg cgcgctattt ccctgcgggg cggcagcggt tgctaattcg attcttgctt
    23461 ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt
    23521 tctgtagcaa aatcctcagc aaactgggcg taacgacatc gtggtttgat ccgctgattg
    23581 gtgccgatat cgttaagcat ctgcagccaa acactaaaat cgtgtttctg gaatcgccgg
    23641 gttccatcac tatggaagtc cacgacgttc cggcgattgt tgccgccgtg cgcagtgtgg
    23701 tgccggatgc catcattatg atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc
    23761 tggattttgg catcgatgtt tctattcagg ccgccaccaa atatctggtt gggcattcag
    23821 atgcgatgat tggcactgcc gtgtgcaatg cccgttgctg ggagcagcta cgggaaaatg
    23881 cctatctgat gggccagatg gtcgatgccg ataccgccta tataaccagc cgtggcctgc
    23941 gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag tctgaaagtg gccgaatggc
    24001 tggcagaaca tccgcaagtt gcgcgagtta accaccctgc cctgcctggc agtaaaggac
    24061 acgaattctg gaaacgagac tttacaggca gcagcgggct attttccttt gtgcttaaga
    24121 aaaaactcaa tgatgaagag ctggcgaact atctggataa cttcagttta ttcagcatgg
    24181 cctactcgtg gggcgggtat gaatcgttga tcctggcaaa tcaaccagaa catatcgccg
    24241 ccattcgccc acaaggcgag atcgatttta gcgggacctt gattcgcctg tatattggtc
    24301 tggaagatgt cgacgatctg attgccgatc tggacgccgg ttttgcacgg attgtataac
    24361 attgccactt ttggacaatt ttgcagacat tttattgtga aaagtcttaa attgttgcgt
    24421 ccgggatcaa ggcgtcccgg acgattcagg agtacaatag gcagataaag gcttaaacgc
    24481 tgttccacag gaaagtccat ggctgttatt caagatatca tcgctgcgct ctggcaacac
    24541 gactttgccg cgctggcgga tcctcatatt gttagcgttg tttactttgt catgtttgcc
    24601 acgctgtttt tagaaaacgg cctgctgccc gcctcatttt tgccaggcga cagcttgttg
    24661 atactggcag gcgcattgat tgcccaaggg gttatggatt ttctgcctac gattgcgatt
    24721 ctgaccgccg cagcaagtct gggctgctgg ctaagttata ttcaggggcg ctggttaggg
    24781 aataccaaaa cggtgaaagg ctggctggca cagcttcctg ctaaatatca ccagcgcgcc
    24841 acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg ctggacgttt tcttgcattt
    24901 gtccgtacgc tgctgccaac catggcggga atttccggtc tgccaaaccg ccgcttccag
    24961 tttttcaact ggttaagtgg attgctgtgg gtcagcgtgg taaccagttt tggctatgcc
    25021 ttaagtatga ttccgttcgt taaacgccat gaagatcagg taatgacgtt cctgatgatc
    25081 ctgccaattg ccttgttaac cgctggcttg ttaggcacgc tgtttgtggt gattaaaaaa
    25141 aaatactgta acgcctgacg attttccccg ttcccggctg ctgtaccggg aacgtattta
    25201 attcccctgc atcgcccgca ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg
    25261 tttaaactca cggctaaatt gcgatgcgct ttcatagccg acgcgcatcg ctgctgcgct
    25321 ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta tgcagacggt aattcttcaa
    25381 atactgcaac ggcgaggtgc tggtgacaga cttaaaatta tggtggaacg ccgatacgct
    25441 catgttggct tctgccgcca gttgctcgac gctcaggttt tcggtgtatt tattctcaat
    25501 tcgtttcagc acgcggctaa tcagactgaa gtgagtctgg cgactgacca gcgccagtaa
    25561 cgcgccgccg caaggtccgg tcagcacgta gtacagaatt tcgcggatga tctgtttgcc
    25621 gagaatacgc gcatccagtg gtcgctccat cacgtcgagt aaccgctccg ccgcgcataa
    25681 aatctcttct gataacgtgg cggagttaat cccgctggct gccatcgacg gctggaaatg
    25741 ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc aaaatatcga cattgagacg
    25801 caaccctgcc agcggcacct ctgacgtggc ataggtttcg cactcaaacg gcaacggcac
    25861 cgtcagcagc aggtattcat tggcatcata acgaaacacg cgttcattga tataaccgat
    25921 tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa
    25981 aggcgtctcg ccatacaaca aacgcacatc gggcaacagt tctgacaaac tattttcttt
    26041 atttttcagt ttattaactt tatccgccag caagcgacaa atctcttcac gtttcatatc
    26101 gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg caattttgta gcatttctcc
    26161 agcactctgg agaaataggc aagacattgg cagaaatgag cattgagagc cagggcgctg
    26221 gcgatcacaa tgaaaaacat caggcagatc gttctctgcc ctcatattgg cccagcaaag
    26281 ggagcaagta atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg
    26341 cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg
    26401 tggcggcagc gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat
    26461 ggacgtgctg gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc
    26521 cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt
    26581 actggacggc accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg
    26641 gcacattctg caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct
    26701 gacgctgcca gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac
    26761 aggcgacaag caggcgttcc attctgcaca tgttcagccg gtatttgccg tgctcgatcc
    26821 ggtttatacc tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt
    26881 acacaccgtg gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc
    26941 agaaggcatt ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa
    27001 ctacgatgtg cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg
    27061 cgctggcgta ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca
    27121 cggtctggat cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg
    27181 cgagaccaag cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg
    27241 ttccgacgat gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt
    27301 aggcgtgccg acccacttct ccgactacgg tctggacggc agctccatcc cggctttgct
    27361 gaaaaaactg gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga
    27421 tgtcagccgc cgtatatacg aagccgcccg ctaagctttt tacgcctcaa actttcgttt
    27481 tcgggcattt cgtccagact taagttcaca acacctcacc ggagcctgct ccggtgagtt
    27541 catataaagg aggaacgtat ggctaatcca accgttatta agctacagga tggcaatgtc
    27601 atgccccagc tgggactggg cgtctggcaa gcaagtaatg aggaagtaat caccgccatt
    27661 caaaaagcgt tagaagtggg ttatcgctcg attgataccg ccgcggccta caagaacgaa
    27721 gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca gagaagaact gttcatcacc
    27781 actaagctgt ggaacgacga ccacaagcgc ccccgcgaag ccctgctcga cagcctgaaa
    27841 aaactccagc ttgattatat cgacctctac ttaatgcact ggcccgttcc cgctatcgac
    27901 cattatgtcg aagcatggaa aggcatgatc gaattgcaaa aagagggatt aatcaaaagc
    27961 atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc tgattgatga aactggcgtg
    28021 acgcctgtga taaaccagat cgaacttcat ccgctgatgc aacaacgcca gctacacgcc
    28081 tggaacgcga cacacaaaat ccagaccgaa tcctggagcc cattagcgca aggagggaaa
    28141 ggcgttttcg atcagaaagt cattcgcgat ctggcagata aatacggcaa aaccccggcg
    28201 cagattgtta tccgctggca tctggatagc ggcctggtgg tgatcccgaa atcggtcaca
    28261 ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc gtctcgacaa agacgaactc
    28321 ggcaaaattg caaaactcga tcagggcaag cgtctcggtc ccgagactga ccagttcggc
    28381 ggctaacgtg caaattctcc cggtggcggt aatgttccgc taccggactt ttcagaaatc
    28441 atttattccc ctcgcgtccc gcccgttgtt actcttcctt gttcaggaat gccaaatata
    28501 aggacatcat catgcagagc cggaagctct taaaagaaca actcatctat atccgggata
    28561 aacgcaacgg agaggtgaaa aacagatgaa aataatactt ctgtttttag cagccctggc
    28621 aagttttacc gtacacgcgc agcctccttc acagaccgta gaacaaacag tccggcagat
    28681 ttatcagaac tataaatcag atgccagtac gccttatttc ggtgaaaccg gagagcgggc
    28741 gataacttca gcgcgtattc aacaggcgct taccctgaac gacaatctta cactaccggg
    28801 caatattggc tggctggatt atgatccggt ttgtgattgt caggattttg gcgatctggt
    28861 gttagaaagc gtagcgataa cccaaactga cgtcgatcat gccgatgccg ttgtgcgctt
    28921 tcgtatcttt aaagatgata aagaaaagac cacacagaca ctgaaaatgg tggcggaaaa
    28981 tggtcgttgg gtc
//
LOCUS       DADKXM010000041        28325 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.041, whole genome shotgun sequence.
ACCESSION   DADKXM010000041 DADKXM010000000
VERSION     DADKXM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.041"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQR05_001739"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQR05_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY5323926.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JQR05_001740"
     CDS             complement(998..2020)
                     /locus_tag="JQR05_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5323927.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JQR05_001741"
     CDS             complement(2034..3536)
                     /locus_tag="JQR05_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY5323928.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQFGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQR05_001742"
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQR05_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY5323929.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="JQR05_001743"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="JQR05_001743"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY5323930.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5852..6193)
                     /locus_tag="JQR05_001744"
     CDS             complement(5852..6193)
                     /locus_tag="JQR05_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY5323931.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6196..9975)
                     /gene="tamB"
                     /locus_tag="JQR05_001745"
     CDS             complement(6196..9975)
                     /gene="tamB"
                     /locus_tag="JQR05_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY5323932.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVLVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9972..11705)
                     /gene="tamA"
                     /locus_tag="JQR05_001746"
     CDS             complement(9972..11705)
                     /gene="tamA"
                     /locus_tag="JQR05_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY5323933.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11911..12540
                     /gene="msrA"
                     /locus_tag="JQR05_001747"
     CDS             11911..12540
                     /gene="msrA"
                     /locus_tag="JQR05_001747"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY5323934.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12863..14206
                     /locus_tag="JQR05_001748"
     CDS             12863..14206
                     /locus_tag="JQR05_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY5323935.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14268..14474)
                     /locus_tag="JQR05_001749"
     CDS             complement(14268..14474)
                     /locus_tag="JQR05_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY5323936.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14799..15353
                     /locus_tag="JQR05_001750"
     CDS             14799..15353
                     /locus_tag="JQR05_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY5323937.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15416..16354)
                     /locus_tag="JQR05_001751"
     CDS             complement(15416..16354)
                     /locus_tag="JQR05_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323938.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16566..17306)
                     /gene="cysQ"
                     /locus_tag="JQR05_001752"
     CDS             complement(16566..17306)
                     /gene="cysQ"
                     /locus_tag="JQR05_001752"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY5323939.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17496..19434
                     /gene="cpdB"
                     /locus_tag="JQR05_001753"
                     /pseudo
     CDS             17496..19434
                     /gene="cpdB"
                     /locus_tag="JQR05_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(19560..19940)
                     /locus_tag="JQR05_001754"
     CDS             complement(19560..19940)
                     /locus_tag="JQR05_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY5323940.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20029..20889
                     /gene="qorB"
                     /locus_tag="JQR05_001755"
     CDS             20029..20889
                     /gene="qorB"
                     /locus_tag="JQR05_001755"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY5323941.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            20998..21963
                     /locus_tag="JQR05_001756"
     CDS             20998..21963
                     /locus_tag="JQR05_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5323942.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22071..22733
                     /gene="ytfE"
                     /locus_tag="JQR05_001757"
     CDS             22071..22733
                     /gene="ytfE"
                     /locus_tag="JQR05_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY5323943.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22778..24190)
                     /gene="cycA"
                     /locus_tag="JQR05_001758"
                     /gene_synonym="dagA"
     CDS             complement(22778..24190)
                     /gene="cycA"
                     /locus_tag="JQR05_001758"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY5323944.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24498..25118)
                     /locus_tag="JQR05_001759"
     CDS             complement(24498..25118)
                     /locus_tag="JQR05_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5323945.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25337..25882
                     /locus_tag="JQR05_001760"
     CDS             25337..25882
                     /locus_tag="JQR05_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323946.1"
                     /translation="MPGRFELKPTLEKVWHALDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25866..26513)
                     /locus_tag="JQR05_001761"
     CDS             complement(25866..26513)
                     /locus_tag="JQR05_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5323947.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKVIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26725..27516
                     /locus_tag="JQR05_001762"
     CDS             26725..27516
                     /locus_tag="JQR05_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY5323948.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27526..>28325
                     /locus_tag="JQR05_001763"
     CDS             27526..>28325
                     /locus_tag="JQR05_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY5323949.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGLPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6943 a         7543 c         6995 g         6844 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acggcttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt ggctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca aactggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agcctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccataattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt gtgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat actcggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttggcgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaaatcg cgagatacat cgcataaaac
     5821 aacgccaccc gaaggtggcg tgcttttatt attacttatc cctgtctaac cagtcgccgc
     5881 tttcaattag ctttaatcca tcgacgggtc gttgataaac gtacatccat gcactcccat
     5941 acggcgtctg aatcaactgg cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt
     6001 cggccagcgt ggcgttgtca atacgataaa cttcaccgtg taccgttccg ttccccggaa
     6061 ctgcgcctgg atagtggccc aggctataca actggtagtt atcgatactg aaatcgccca
     6121 gtaactgggc attggtcatc cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga
     6181 caaatattcg cattgctaaa actcgaactg atagagcaaa tccagtgcct ggtctacacc
     6241 agacacggct tccagatata gcttaggcat caggcgataa cgtaacgtga gtgttgctat
     6301 agagtcaaat atacccacgc cgtatttcac ttgcagacct ggcaatacat agccgctgac
     6361 cactacctgg gaggagtcgc ctactcccag ggtgtcgagc gctaaattgc ttacgccaaa
     6421 cgtctcgccg attttaccca caatctggcc actttgcgca acccccagac caatcagcat
     6481 cgaggtcatt gccgcactgt cgctctgatc gctctccagc ccttgtccac gtagcaaata
     6541 agacaatgca gcttgttgcg acatcgccgg gtcagagaag atctccgctt tcggttcgtc
     6601 cgccagacca gtgacgcgaa ctccggcgat tacatcgtct tctgtagcat ccgggttacg
     6661 aatagcttca atattaagat acggttgatc cggcggacca gagaacagta actcaccctt
     6721 acgcacaatc agatcctgac cataggcatg gaagcgccct tcagggatgt tgatctgccc
     6781 gttcaggccc agcccttgtt tgtcctgtac gacgttaaga tcgcccgtca gccgcgcttt
     6841 caggccaaag gcgtcaatgc gcacattgtt gccaacgtgg acaatcaggt tactgttaat
     6901 cggaatcgac gccgttttcg gctcttccgg ttgcaggtta tcgttaagca tcaccacatc
     6961 gctggagacg cctactgcgc tttccggcag atcgtgcacc acgatgcgcg cccacgggac
     7021 atccacgcga ccatcgaggg taaacaggtt tggtgtagcc tcgaatacaa catctggcga
     7081 tacatccatt cgtaccatcg gcggcacggt gatccgcact ttactgcctt tcgccgttac
     7141 tcgtgcccgc cagttttcaa tttggctcca gtcggcatca ccgttcaggt agatttcacc
     7201 ctgctgggtc cgtactgtac cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag
     7261 ctggctcggc tgcatatcaa acggcataaa gttgccgtcg atatccacac ccgtaacctg
     7321 aagctgacca aacaactgcg ggctttgcac atcaccaccc agacgcaagt tggcactcac
     7381 catccccgct gctttttccc cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt
     7441 acggatattg acgttgccac caagattacg gcggccttgc ggatcggtca cctgcacctg
     7501 tccatcaaac tggccgttat tggtcaggcg gatggtccag cccaattcgg cacggttgtt
     7561 acgcaattcc gccgtcagat tcagtgtctg aaacgccacc ggcagcgccg catcgttgac
     7621 ggtttgcgtt acctgcacgt tacgccccga aagggtgata ctgccctgcg gcagcccctc
     7681 tttcgtggtg tcccaggcaa catccgcttt acccgtgaag ataccgctgg cctgagtggt
     7741 ttctggcata aacggtttca gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg
     7801 cccttcggcc cccgcatcga tagtttgcgg cacgcacagt tccgcattcg ggttaagcca
     7861 gcagtgtggc ccgatgctga ttttttgctc cttattgcgg taatccagcg caatatcgcg
     7921 ggtcagcgac cacgggccga ccggcgtctg gaagcgggta ttgctaagag ttcccttcca
     7981 gcgttcttct ttgcgatcaa aacttcctgc cagattaagc tgcccggaga caggctcgcc
     8041 ctgaatccgc aactgtagct cgtgctgctt ttcgctgcct ttggcattca gggtgacgag
     8101 gttgatattt acatccggct gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat
     8161 ctgatcggtg gatttaatgt cgccttccac gcgaacctgc gccacggaaa gttcctgcca
     8221 gcgcaggccg cgcgcggtga tatctgccag tagttgtggc gcttccaccg tgccgcgtac
     8281 tttcaccagc cctttcgctg taccgccaag ccccggcagc gcgttatcca gccccggcgc
     8341 gttgatggtg gcatcaagat tgagatcttt tacccccagc tcgcctttca cttcggcact
     8401 gtttggcccg agttccagat gaagcccagg gatcatccac tgcatataac tgttgccttt
     8461 cagcgtgccg tcaacgttca ctttgttctg tttgacgtta ccggtcagct tcagttctgg
     8521 cacctccatc tgccaggtgc cgccgtacag gctaccgcgg gttttaatca agccattgag
     8581 tttcgacggc cactccggga actctttggc ggtgttaatg ccattaagcg ttagctcacc
     8641 gcgccaacta atggcctgct gccagtcgag caacgccttg agttcagttt tcccttccag
     8701 cgccgcgacg gtgagtttgt cgagattgac ctgctgttca ttacctttgg catcaagggt
     8761 aatggtggct ggcgggatct cctgtccctt cactgccgta cgcatagaga gcgtgtaatc
     8821 ggtcattttg ccggtaagtt tcagtttcag atcatccgcc tgatactgct tctcaccagt
     8881 gaacggccag taaagctgtt tgctgttcac ttccacgttg agtggcaatc cggcttccgc
     8941 cagtcgcgtc tgggcgcgta aatccatatc caccggaccg gaaaggttaa cgcctatctc
     9001 cagctgttcg cgcagcgcgc cgcccacttt cagcttcacc ttttcacctt tcaacggctc
     9061 cacgttcagt gtgctgttga gggtgatatc caccggccag ttgtctgaca gctgcgccgt
     9121 gccgctggcg ttgacgatcc cctggttgga atcgatatcc agggcgtcca gtttagtatt
     9181 gccgtcaatg ctgctcactt tcagcagcat ggtgcgcacg gtgatgtccg tgtcgcccgt
     9241 cacgcgcagc tgctcgccct taaactcttc aatgttcagg ttaagcggca aatgcacgtc
     9301 ggtcatttcc ggcaataccg ggcgagaaaa gagatctttc agcgtttcgc cgagcggctt
     9361 ttcctccggc tgcggatttt caattttcgg ttcgaccact tcttcctgcg ccacttccac
     9421 cactttcggc agagcaatca gcaggccttt cagcgacgtc ggtttcaggg tcagggtttt
     9481 ctcctgccag ttcaggccgg aggtgaagtc catcaccaac accgtggtgt catcaatctt
     9541 gatgttgacg ttgtccagcg ctacccgtgt cagggtgatg ggatacggcg tggagagatc
     9601 cagcggaccg ctatcttcct cttcttcaac ctgttcagaa ggaggcattt ttttactgtc
     9661 gatgttgacc tgaatgtctt tcagcgccag gtcattaata caaacgctac tgttccacag
     9721 gcactcaaga ccgaccgcca gatgcagatt acccgctttt accgccacgc ctggctgctc
     9781 ataacgaacg tcagacaagg tgagatcgcg ccagccgccg gtgaccttgc caatatccag
     9841 tcctggcacc cagcgatccg ccgctttaaa tacaagatgc aggccgctgg tggtgcccac
     9901 cagaaacgcc accgatccca gcaacagtaa gataacgata accacgccga ggctgatttt
     9961 tttccataaa ctcataattc tggccccaga ccgatgtaaa actgtaaccc gtgttcgtct
    10021 ttatccgcga ccggtacggc aaaatcgagt ttgattggcc cgaccggcga ttcccagcgt
    10081 acgccgaccc cggtaccggt tttaaagtcg ctgcggcgaa tatcgcttac cgcttcgcca
    10141 ctatcgacaa acaccgcgcc ccaccatttt ccggtcacgt tgtactggta ttccagcgat
    10201 ccggttatca acttcgaggc ccctttcagg tcaccgttgg cgtatttcgg ggcgatagat
    10261 ttgtatttgt agccgcgaat actgcgatcg cccccggcga agaaacgcag atccggcggt
    10321 actttgtcga aatcaccggt ttcaatccag cccagcgtgc cgcgtgtcac aaagcgatgg
    10381 cgatcgtaca gtgtgcggat ccagacgttc tgcgcctgga aaacggagaa atcgacatct
    10441 gaaccccagg ccgtgttgga gtagtcgata gagtagcgtt gcgagtcgcc ccaggttggc
    10501 atcaggccac cacgagaacg cgtgcggcta atcatcaccc caggataaaa cagcatcgtg
    10561 gtgttggtaa tttcaccctg ggtaaagtgg tcgaggctcc agcgcaggtt aatggcacgc
    10621 tgccagccgc tggagagatc ccagtagcga gaagccacca gcgtagtgga gtcagattcg
    10681 gtatcgttca ggtcagtgcg cttaaaaccg ccctgcacca aataatattg ttccagtgga
    10741 ttcttcagca gcggcatttt atagctgaag tcgagggtct gttccggcgc ggaaatactg
    10801 gtactggtgg tcaaactgtg accgtatgag ttcatccacg gctttttcca cgtcgctttc
    10861 acgcgcggtc ccacgtccgt agagtaaccg accccggttt cgatggtgtt ttctgttcgc
    10921 ggcgaaacca cgcccgtcaa tggtaatact ttcgtttcgc gcgctttatc aaattgtgga
    10981 gccaccacca ccgagttaaa ccagccggta gcagaaagtc ggcggttcag ttccgccaga
    11041 tctttcgatt catactcatc gccctcttta aacggcacca gattttgcag gtattcatcg
    11101 cggatttgtg atccttcaaa ggtcacatgc ccaaagcggt aacgttcgcc actgttataa
    11161 tcaatatccc agaaggcttt atgcaggccg agcgcaatgc ccagctgcgc tttggtaaat
    11221 tcgctatcga aataaccttt acgcaacgca atactggtta aggacttttt gaaattttca
    11281 taatcgccct ggttcagtac cgtaccaata gccgggcgag tatcgagcaa tttcaaatag
    11341 tctttatcgg tccgcgcgcc gccgcgcaat accacatcgg tgccgccaat taacaccggc
    11401 acacctggcg tgactttggc gatcaatacc tgccgccctt tctttggcgg tggacggaga
    11461 tcaaattcaa tggtcggctg gtaataaccc agcgctttca gaccttcacg gatggcatca
    11521 tcgacgcgtg cgcgaaagcg acggtctggc gtcacttcat cactttcaat cgtagaaagc
    11581 tgcgcacgaa cgttcttttc cagctgtccc gataacccct cgacctgtag acggacgttc
    11641 gcggcgacgg cagatccgct taagcagagt aagcttacac agcataactg tcggatatag
    11701 cgcacatttt ctcctgaata tccttttttc ctgcccctgg aacgccgtta aacggcttaa
    11761 caaaaatcca gtaatatgga ttaaaaagca gactaaaccc caaatatttc ttatgtttta
    11821 ctttagacct attcacggtg gttattgtgt gcaaatacgc ctcttgttac aaccttaacc
    11881 ccaatgaccg attttcggga gagcgacacc atgagtttat ttgataaaaa gcatctggtt
    11941 tcccccgccg atgccctgcc tggacgtaac accccgatgc ccgtagccac gctgcatgcg
    12001 gtcaacggtc actcaatgac caatgtacct gacggaatgg agattgccat ttttgcgatg
    12061 ggttgtttct ggggtgtgga gcgtctgttc tggcagttac ccggcgttta cagcaccgcc
    12121 gcaggctata ccggcggcta tacgccaaat ccgacttatc gggaagtgtg ctccggtgat
    12181 acgggtcatg ccgaagcggt acgcattgtt tacgatcctt ccgtcatcag ctatgagcag
    12241 ttgctacagg tattttggga gaatcacgat cccgcccagg gaatgcgtca gggcaatgac
    12301 cacggcacgc agtatcgttc agcgatttat ccgctgaccc cagaacagga tgccgcagct
    12361 cgcgccagtc tggaacgttt tcaggcggcg atgcttgccg ccgatgacga tcgtcacatc
    12421 accacggaaa tcgctaacgc cacaccgttt tattatgccg aagatgacca ccagcaatat
    12481 ctgcataaaa acccatatgg ttactgtgga attggcgtct gtctgccgcc ggaagcatag
    12541 cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc tgatgcggct
    12601 taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag gactgcgtag
    12661 gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc
    12721 tctggcgact ttacagtacc ttacgctata ctagccactg aaaatgccgg ttcactttct
    12781 tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt ccgaggatct
    12841 ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc
    12901 tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca aaatcaaact
    12961 taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa
    13021 tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga ttctcggcgg
    13081 tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc
    13141 ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt
    13201 tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc
    13261 tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc tggtgtggtt
    13321 cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga
    13381 tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt
    13441 acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc
    13501 gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag
    13561 cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat
    13621 tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca
    13681 aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt
    13741 aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat
    13801 gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat
    13861 gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg
    13921 gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata ttgacgagtt
    13981 cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc tgcgtaagat
    14041 cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga
    14101 taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca ccgccctttc
    14161 gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc cagaaacatc
    14221 aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta agccatctct
    14281 gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc
    14341 ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac gtccttgatg
    14401 tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt
    14461 tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc
    14521 ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc gggcaatgtt
    14581 tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc
    14641 gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa
    14701 ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac tcagaccgaa
    14761 gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg
    14821 cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa atcggtcagc
    14881 gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa gatcagttta
    14941 gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg
    15001 ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga
    15061 agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac gcgattccgg
    15121 gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat ccctggtcgc
    15181 agtttattgt cgatagcaat ggtgtcgcgc gcggtgcctg gcagctggat gaagaaagtt
    15241 ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctca
    15301 caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa taatggcaat
    15361 aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag gggaattaag
    15421 tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt ttaaacgcct
    15481 caatggtcga tttatatttc tcgcaaatct tttctttggt aatattacta tctgcggcca
    15541 gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg caacctgcat
    15601 taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca gaggaaccat
    15661 tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat tctatgacta
    15721 tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat tctttaattc
    15781 caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact acatcctcca
    15841 ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct ttaatccttt
    15901 ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc atacaatctt ctgttttgtt
    15961 ctaacggaaa ctcgcggttt ttgaaatcca tgaatgagtt atactcattt attgttatgg
    16021 gaatttcttc gtattcaaac ccactgcctc gcaatgtcat tgtatattca gcaccgttat
    16081 ctttaaggct gaaactgata agaaaattat ccatggcatt aacagtttca ttttttgatg
    16141 aaagcacttg gtaactattt ttatcaatct gtgttgcgca acgaaaggac tcccgcaaag
    16201 ttaacagact atcataattc gaaacccaat cttttttctt aaatgcatgc tgaatattat
    16261 taaaaaaatt gtttgaatgt gtttttgtat gcgatgactt tacaaataac tccaccgcac
    16321 caataaaatt attgtgtctg atcctgggta acatatggct accttatttt atttggttat
    16381 tttatgtcgc cattaaatat ctcattatag aagtaaacta tcaaaaaacg ctcattatta
    16441 aaatgtaact tattagcaat tgaatttttg cactttaaat acaatctctt tttatttata
    16501 actttttagg ttgggataaa tcaggatggc agaatcagga aatacactgt ttctgccatc
    16561 tgaatttagt aaatggacac tctgaacccc ggattcagga acgactcgcg cggagtgtaa
    16621 tccagcggtt taccctgcca gtcgtgaacg tgcgctccgg cagctgcagc cacagcatgt
    16681 ccagcggcgg tgtcccaaat attcgttggt ccgaagcgcg ggtacagctg cgcctgtcct
    16741 tccgccacca ggcagaattt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc
    16801 tgttgcagat actctttcag ctccgcatct gcatgggaac ggctgataac caccagcggc
    16861 gggcgcgcat cgcggacctg aatcagcttg cgcacaccgc actcttcttt ccaggccttg
    16921 ccttctgccg cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt
    16981 ttgccatggt caatgagcgc aatgttaacg gtgaattcgc cattacgttt aataaactct
    17041 ttagtaccat ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa
    17101 ccgggaggat cttcttcaga aaggaccgga atatccggtg ccagcgtacg taaaccgtcc
    17161 atgataacgg tgtgagcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg
    17221 acgacgtcca tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc tgcattccgt
    17281 gcaagctggc atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt
    17341 attgttttac ttatacccta tcgttaatga atgcgccaac tgtgatagtg tcatcatttt
    17401 caaagcgtaa aattgtggca ttcttcactg ttctataagt aaggcgttta ttctcccttt
    17461 tctttcgtat tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc
    17521 tggccacgct gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa
    17581 ccactgatct gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa
    17641 aattcggact ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa gtgaaaaaca
    17701 gcgtactggt cgataacggc gatttgattc aggggagtcc gctggccgat tacatgtcgg
    17761 cgaaaggatt aaaagcaggt gatgttcatc cggtttataa ggcgctgaat acgctggatt
    17821 atacggtcgg tacactcggc aatcatgaat ttaactacgg tctggattac ctgaaaaatg
    17881 cgctggcggg agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac
    17941 agccaatgtt tacaccgtat ttaattaaag ataccgaagt ggtcgataaa gacggaaaaa
    18001 aacagacgct gaagattggc tatattggcg tcgtgccgcc gcaaatcatg ggctgggata
    18061 aagctaattt atccggaaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg
    18121 tgcctgaaat gcgcgagaaa ggtgccgatg tcgttgtcgt tctggcgcat tccgggctgt
    18181 ctgccgaata aagtaatggc ggaaaactca gtttattacc tcagtgaaat tccgggcgtt
    18241 aacgccatta tgtttggcca tgctcacgcc gttttcccgg gtaaagattt tgctgatatc
    18301 gaaggggctg atatcaccaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg
    18361 tggggcgatc atcttggggt ggtcgactta caactcagta atgacagcgg taaatggcag
    18421 gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa aaaatccctc
    18481 gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag
    18541 ttcgtcagca agccaatcgg taaatctgcc gacaatatgt atagctatct ggcgctggtg
    18601 caggacgatc cgaccgtgca agtcgtgaac aacgcgcaaa aagcgtatgt cgaacattac
    18661 attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa
    18721 gttggtggtc gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg ccagttgacc
    18781 ttccgtaatg ccgccgatct ttatctctac cccaatacgc tgattgtagt gaaagccagc
    18841 ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgctcct
    18901 aacagcacga aaccacagtc actcatcaac tgggatggtt tccgcactta taactttgat
    18961 gttattgatg gtgtgaatta tcagattgat gttacccaac ccgcccgcta tgacggcgag
    19021 tgccagatga ttaatgccaa tgcagagcgg attaagaacc tgacctttaa tggcaagccg
    19081 attgatccga acgctatgtt cctggttgcc accaataact atcgcgctta cggcggcaaa
    19141 tttgccggta cgggcgacag ccatatcgct tttgcttcac cggatgagaa ccgctcggtg
    19201 ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcatcc ggcggcagat
    19261 aacaactggc gtttagcacc gatagctgcc gataagaaac tggatatccg tttcgaaact
    19321 tccccgtcag ataaagccgc agcgtttatt aaagagaaag ggcagtatcc gatgaataaa
    19381 gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact
    19441 tctttttcgg cctataaatc aaccgcatcc ggcatttatt ggcggatgcg atgctgccgt
    19501 atcttatcca ccctacaagc catgaaccgt agaccggata agcgcagcgc atccggcagt
    19561 tatgccgcac gttcatcccg caccgccaac acctcgggca aattcaactc aatccagtcc
    19621 gccagtgcag caaccttttc gctcacctgc tcgcccagcg gcgtgaggct atattccaca
    19681 tgcggcggca ccaccggata cgcgatacgg ttaagaaaac catcctgttc taacgcctgt
    19741 aacgactgcg caagcatctt ttcactcacc ccaccgattt tgcgccgcag gtcgctaaag
    19801 cgatgagtac cttcgcgtag cgccaccaga atcaacaccc cccaacggct ggtgacgtgt
    19861 ttcaacacct cgcgcgacgg gcactgttcc gcaaagagat taccctcttt cagttgttgt
    19921 gacaggctaa cctgactcat ttcatactta cctttttgta cgtacttact aaaagtaagt
    19981 ttaggtgtta gcgtatttaa acacaagaca aaacgatgga gacttcccat gatcgctatt
    20041 actggtgcca ctggccaact tggtcactat attattgaat ccttgatgaa aacggttcct
    20101 gccagccaaa tagtggctat cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa
    20161 ggcattaccg tgcgtcaggc tgactacagc gatgaagccg cactgacatc tgcacttcag
    20221 ggagtggaaa aactactgct gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat
    20281 cgtaatgtta ttaatgccgc aaaggcggct ggcgtgaaat ttatcgctta taccagcctg
    20341 ctacatgcag atacctcccc gctcggcctc gccgatgagc acatcgagac ggagaaaatg
    20401 ctggctgatt ctggcatcgt ttacaccctg ctgcgcaacg gctggtacag cgaaaactac
    20461 ctcgccagcg ccccggcagc actggaacac ggcgtattta tcggtgcggc gggcgatggc
    20521 aaaatcgcct cagcaacgcg ggcagattat gcgacagctg cggcacgcgt gattagcgaa
    20581 gccggtcacg aaggcaaggt ttacgaactg gcgggcgata gtgcctggac gttgacacag
    20641 ttagcggcag agctgaccaa acagagcggc aaacaggtta cctatcaaaa tctgagcgaa
    20701 gccgatttcg ccgcggcact gaaaagcgtc ggactgcccg acggactggc ggatatgctg
    20761 gcggattctg acgttggcgc atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc
    20821 aaattgattg gccgcccaac gacaacgtta gccgaaagcg taagccatct ttttaatgtt
    20881 aataactagt taattaaagt ggcatcctcc cgcatcctct ctgataatga cgggatgccg
    20941 ggagcaatca tgtctgcttc ctgaacgttt cttctgacag accaatggat gccagtaatg
    21001 attagcggcg tgctgtacgc cctgttagca gggttgatgt gggggcttat ttttgtcggg
    21061 ccgttgatcg tgccggaata cccggcgatg ttgcagtcga tggggcgtta tctggcgtta
    21121 gggttaattg cgctacccat tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt
    21181 cgggactggc ttaccgcctt gatgctcact atgatgggca acctcattta ttacttctgc
    21241 cttgccagtg ccattcaacg tactggcgcg cctgtttcca cgatgattat cggcaccctg
    21301 ccggtggtga ttcctgtctt tgccaatctg ctttatagcc agcgcgacgg caaactcgcg
    21361 tggggaaaac tcgccccggc actgatttgt attggcatcg gcctggcgag tgtgaatatt
    21421 gctgagttaa accacggact ccccgatttt gactgggcac gttatacctc tggcatcgtg
    21481 ctagcgttag tttccgtggt ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg
    21541 cgggaaaatc ccgacaaaca tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg
    21601 ccggtttctc tcatcggcta tctcgtcgcc tgttactggc tgaatataca aacgccggac
    21661 ttctccttac cttttggccc ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc
    21721 gtgctttgct catgggttgg cgcactctgc tggaacgtcg ccagccagcg attaccgaca
    21781 gtgattctcg ggccgctgat tgttttcgaa acgctggcag gtttgctgta caccttttta
    21841 ctccgccagc aaatgccgcc gctaatgacg ctgagcggta tcgcgctgtt agtgattggc
    21901 gtggtcatcg cggtcagagc aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt
    21961 tgacatgctg gcagtgagtt aaataagcct ctgctacgta agggttatag cttttgcctt
    22021 aaaggtgtat ttaaaataca tcttatctta ttaagaatga ggtatcagct atggcttatc
    22081 gcgaccaacc tttaggtgaa ctggcgctct ctatccctcg cgcttcggct ctgtttcgta
    22141 aatatgatat ggattactgc tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta
    22201 aagaactgga tgttgaggtc attgaagctg aactggcaaa gcttgctgaa caaccgattg
    22261 agaaagactg gcgtagcgcc ccgctggcag aaatcattga ccatatcatc gtgcgctacc
    22321 acgatcgtca ccgcgagcag ctgccggagc tgatcctgca agcgactaaa gtcgagcgcg
    22381 ttcacgccga caaaccgagc gtgccaaaag ggctgacaaa atacctgacc atgctgcatg
    22441 aagagctttc cagccacatg atgaaagaag agcagatcct cttcccgatg atcaaacaag
    22501 gcatgggcag ccaggcaatg gggccaatca gcgtaatgga aagcgagcac gatgaagcgg
    22561 gcgaactgct ggaagtgatc aaacacacta ccaataacgt cacaccgccg ccagaagcct
    22621 gcaccacctg gaaagcgatg tataacggca ttaatgaact gattgatgac ctgatggatc
    22681 acatcagtct ggaaaacaat gtactgttcc cacgcgccct agcgggtgag tgatctaaaa
    22741 gctggatggc attgcgccat ccagcatgat aatgcggtta tttccgcagt tcagcagccc
    22801 gcttcttacc aataaacagc cagcccaacc ccagcgcgat aaaccacagc ggggtaacca
    22861 gcagcgcctg gcgagtgtca tcttccagtg tcagcaacac gaccacgaac acaaagaacg
    22921 ccatacatac ccagcacatc agcttgccga gcggcatctt gtagatcgac ttttcatgca
    22981 gatgaggacg ctgtttgcgg tacaccaggt acgagcaaag gataatcgtc cagacgaaca
    23041 taaacagaat cgcggaaacg gttgtaatca tcgtgaacgc gccaatcacg ctaggattca
    23101 catacaacat caccacgccg ccgagcagac agatacacga gaaggtcaac cctttcgcgg
    23161 gtactgcgcg cttagaaagt ttagcgaacg ctttcggcgc aacaccttcc tgcgccagac
    23221 caaacagcat acggctggta gagaagacac cgctgttagc ggaagacgcc gcagaggtca
    23281 gcaccacaaa gttgatcacg ctggcagccg caggcaaacc taccaacaca aacagctcaa
    23341 cgaacgggct tttctccggg actaccgaac tccacggtgt cacggacata atcacaatca
    23401 gcgcgaagac gtagaacata atgatacgga tcggaatgga gttaatcgcg cgtggcagtg
    23461 atttctccgg atctttggtt tccgcagctg ttgtacctac cagctcaatc cccacgaaag
    23521 cgaaaaccgc tatctggaat ccggcaaaga agccacttaa acctttcggg aaccagccgc
    23581 cgtcattcca caaatgcgcg aatgacgctt ccacaccggt cggtgactga aagtgcatcg
    23641 ccaccatgac caggccgacg acaatcagcg acacgatggc gacgattttg atcatcgcaa
    23701 accagaactc catctcaccg aacattttca cggtggcaag gttgagcgtc agcagcagca
    23761 ctatcaccgc cagcgaggcg acccagtcgg agagatcggg gaaccagaac tgagcataag
    23821 ccgtgatcgc caccacgtct gccataccgg ttacaaccca gcagaaccag taagtccagc
    23881 cggtgaaata tcccgcccac ggccccagta aatcggaagc gaagtcacta aaagatttgt
    23941 attccagatt cgaaagcagc aattccccca ttgcccgcat cacgaaaaag agcataaaac
    24001 caatgatcat ataaacgaaa atgatcgacg gcccggcaag gctaatcgtt ttgccggacc
    24061 ccataaacaa cccagtacca atggcaccgc caatggcaat aagctgaata tgtcggtttg
    24121 tgagattgcg ccgtagcgac tgttcagccg gagcctgatc atcggcaacg acttttacct
    24181 gatctaccat gttttttctt cctgtacctg tctgtgttgt tcaggctcta cggcctttag
    24241 tcagtctatg atacgacgaa tctgtatcgg gactcatcga tattaggtaa caatacgcgg
    24301 gatgaatact aagatttaga tctaatgtta attttatgtt taaagtgagt gtcatatcac
    24361 ggtgataacg cgaaacagct cacaaaaata cacgcttacg gggtctttgc aacacaaaat
    24421 agcgccgttt ggcaaactga agggtttatt gctgaatgcc tgctcccctc tggttcgagg
    24481 ggaacagaat gcgctgctta gaggatttcc agcagttcga cttcaaacac cagggtgctg
    24541 aacggaggga tggatgcacc tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc
    24601 agttcccatt tagaacctac cggcatcaga gtcagtgctt caatccagcc agggatcacg
    24661 ccattaaccg ggaattcagc gggttcacca cgagcaacgg agctgtcaaa cacggtgccg
    24721 tcgatcagtt taccggtgta atgaacacga acgcggtcgg tacgtgccgg aattgcgcct
    24781 tcaccctggt tgatcacgcg gaattgcagg ccagattcgg tgctattcac accttctttt
    24841 ttggcgtttt cttccaggta tttcacacct tcagcagcca tcgcctggaa acgctgacga
    24901 cgaacggcat cggcgcgttc gtggatttca cgcagcgcgc gatgcaccac atcaaccggc
    24961 acagccggat gtttgccttc cagcgcatcg gcaatacctg caaccagtgc ttctggcagc
    25021 agcccttcca ggccagattc actcagttgt tgccctacct gcaaaccaat gccgtagctt
    25081 gcttgcgctt cgatggtgtc aaaagttggg gtggtcatcg tatttccttt cttagggtat
    25141 aaaagtagcg ggcagcatat cagccacact tctttgggta aaactttgtc gcaggaatga
    25201 tgacaaatcc cggggaaaaa gaaacaatag caatatcccg tatgaatata ataaaggcgg
    25261 gtttaccacg gacatcatag cgttagctta ttcataggct atgattgagg aacaagacgt
    25321 ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg
    25381 gcacgcgctg gacaattttc gcttcatgga cccgctgccg ccgatgcatc gtcgggggat
    25441 cattattgcc gccattgtat tggtggtcgg atttctgctt ccatctgatg atacgcccaa
    25501 cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac aaccgccgac
    25561 agaagaacag ccgcaaacta cccaaacgca accattccag ccagatagcg gaattgataa
    25621 tcaatggcgc tcttaccgtg tcgaacccgg taaaacgatg gcgcagctgt tccgcgacca
    25681 cggactgccc gctaccgatg tctatgcaat ggcgcaggta gaaggcgcgg gtaagccgct
    25741 gagtaatttg caaaatgggc agatggtgaa aatccgccag aacgccagcg gcgtggtgac
    25801 gggcttaacc attgatacgg ggaataatca acaggtcctg tttacccgcc agccggatgg
    25861 cagttttatt cgtgcgcggt agatgaaggg tcaaccaaaa gccagctaag gattgtctct
    25921 ttgatgacct tacgtctggc ttccagtgcc tggggggaaa ccatactgat atgataaacc
    25981 aggccaagcg tatgctggtt gatcaaataa tatcccccaa gcgcagcaat attaatattc
    26041 acctgcaatg ggtcgatacc tggcttaaaa atattgtatt tcttcccttc atccaataag
    26101 gattccatta actgcaagtg cgcatggtta atttctaaga gacgctgtga ttttgcataa
    26161 tgcacacctt tactttgatt ttcactatgc acaattttta aaaaccaagg attggcaata
    26221 taataatccc atgtaaaatc aataactttc tcaatggctt tttgtggcgg aagatctgag
    26281 aaatcaaggg ccttttcttt ttgtcgaata tcgttccagg tatattccag cacttcgata
    26341 aataaatttt ctttattacc aaagtggtga taaaccgtct gtttactaca ccctgctgcc
    26401 tttacaatat tatctactct ggccccttca taaccatatt cggcaaattc attaaccgca
    26461 catagcagca atttttcctt caggctctgt acgttgctgg tatcaatata cataacgctt
    26521 ccccacgacc tcttcgtggt gactatcata acctgatttg atttcagctt cagaaaaaag
    26581 ggcaaaaact tgtactttca tcaggttatc aaatgtcctg tgatcgccat cgcatatcaa
    26641 ccaactagat ggttgatctt tttatcctcc agtcgcaagc gaatcatcac caaggaaaca
    26701 aaattcaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt gattaccgga
    26761 gccgctcgcg gtattggaaa gggctgtgcg caggagcttg ctcgtggcgg gtttaacctg
    26821 ctgattaacg atcgtcccga tgccgacagc gtagaaaaac tgcatatcac acaacaagaa
    26881 tgtttggcgg aaggcgtgga agtgatttgc ttccctgctg atgtcggtga tctatctctg
    26941 catgaggaga tgctcgacgc ggcgcaaaac cagtgggggc ggctggactg tctgctcaat
    27001 aacgcgggga tttcagtgaa aaaacgcggt gacctcctcg acctcgaacc agacagcttc
    27061 gatcaaaaca ttgccattaa cacccgcgcg ccgttcttcc ttgctcaggc attcagtaag
    27121 cgcttactgg cacagccgaa accggaagcg gagctgccgc accgctcgat tattttcgtc
    27181 agttccatca acgccatcat gctggcgatg aatcgcggcg aatacaccat tgccaaaacc
    27241 gccgtttctg ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca aattggcgtt
    27301 tatgaagtgc gccctggtct catcaaaact gatatgacta ttccggcaac ggcttattac
    27361 gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc ggctgatatt
    27421 gcttccaccg tccgcgcgat ggcggaaggt aaactgattt acacctgtgg tcaggcggtc
    27481 gcaatcgacg gcggcttaag tatgccgcgc ttctgaggct gaatcatgag agatctgtta
    27541 caacgcccgg atttattcag tatcaatacc gcgacactgg gctataaaac gccgctaccc
    27601 gccattattg atgcctgcgc cgcacgcggt attggcgcaa ttgccccctg gcgcagggag
    27661 ttgcagggtg aagatctcca gcaaatcacc cgccagcttg ccgccagtaa catgagcgtt
    27721 tccggtttgt gccgtagcac ttactacacc gcgccgacac tggcggaacg caaactggcg
    27781 attgatgata accgccgggc gctggacgac gcagcggtgc ttaacgccgc ctgttatatg
    27841 caggtggttg gcggtctgcc aacgggcact aaagatctat atgaagcgcg agaacaagtg
    27901 aaacaaggga ttcgccagtt gctgccacat tcgaaagatg tcggcttgcc aattgcgctg
    27961 gagccactgc acccgatgac agctgccgat cgctcttgcc tgtgcacgtt acgtcaggcg
    28021 ctggactggt gtgatgaact cgatcccgat ggtgagtttg gcctcggcgt ggcggtggat
    28081 gtttatcacg tctggtggga tccggatctc gccagtcaga tcctgcgtgc cggaaaacgc
    28141 atcctcgcgt ttcatgtttc cgactggtta gtgccgacca ctgatctggt caatgaccgt
    28201 ggtatgccgg gagatggcgt catcaatatt ccgtcaattc gtcggctggt tgaaaacgcc
    28261 ggatttaatg gtgctattga actggaaata ttctcgcctt actggtggca gaaagatatt
    28321 aacag
//
LOCUS       DADKXM010000042        28154 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.042, whole genome shotgun sequence.
ACCESSION   DADKXM010000042 DADKXM010000000
VERSION     DADKXM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.042"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JQR05_001764"
     CDS             complement(<1..209)
                     /locus_tag="JQR05_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5323950.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQR05_001765"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQR05_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY5323951.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQR05_001766"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQR05_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY5323952.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQR05_001767"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQR05_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY5323953.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQR05_001768"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQR05_001768"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY5323954.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQR05_001769"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQR05_001769"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY5323955.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQR05_001770"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQR05_001770"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY5323956.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JQR05_001771"
     CDS             complement(4598..5320)
                     /locus_tag="JQR05_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5323957.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6281)
                     /gene="fkpA"
                     /locus_tag="JQR05_001772"
     CDS             complement(5487..6281)
                     /gene="fkpA"
                     /locus_tag="JQR05_001772"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5323958.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKAADSAKK"
     gene            6502..6720
                     /locus_tag="JQR05_001773"
     CDS             6502..6720
                     /locus_tag="JQR05_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY5323959.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6769..7359)
                     /gene="slyD"
                     /locus_tag="JQR05_001774"
     CDS             complement(6769..7359)
                     /gene="slyD"
                     /locus_tag="JQR05_001774"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY5323960.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7454..7654)
                     /locus_tag="JQR05_001775"
     CDS             complement(7454..7654)
                     /locus_tag="JQR05_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY5323961.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7664..9469)
                     /gene="kefB"
                     /locus_tag="JQR05_001776"
     CDS             complement(7664..9469)
                     /gene="kefB"
                     /locus_tag="JQR05_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY5323962.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKTQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9469..10020)
                     /gene="kefG"
                     /locus_tag="JQR05_001777"
     CDS             complement(9469..10020)
                     /gene="kefG"
                     /locus_tag="JQR05_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY5323963.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10151..12064
                     /locus_tag="JQR05_001778"
     CDS             10151..12064
                     /locus_tag="JQR05_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5323964.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12064..13086
                     /locus_tag="JQR05_001779"
     CDS             12064..13086
                     /locus_tag="JQR05_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY5323965.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13080..13298
                     /locus_tag="JQR05_001780"
     CDS             13080..13298
                     /locus_tag="JQR05_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY5323966.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13352..14221
                     /locus_tag="JQR05_001781"
     CDS             13352..14221
                     /locus_tag="JQR05_001781"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY5323967.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14276..14680)
                     /locus_tag="JQR05_001782"
     CDS             complement(14276..14680)
                     /locus_tag="JQR05_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY5323968.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14982..15614
                     /gene="crp"
                     /locus_tag="JQR05_001783"
     CDS             14982..15614
                     /gene="crp"
                     /locus_tag="JQR05_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY5323969.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15665..17755
                     /locus_tag="JQR05_001784"
     CDS             15665..17755
                     /locus_tag="JQR05_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5323970.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVILSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17822..19042)
                     /gene="argD/dapC"
                     /locus_tag="JQR05_001785"
     CDS             complement(17822..19042)
                     /gene="argD/dapC"
                     /locus_tag="JQR05_001785"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY5323971.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19128..19691)
                     /gene="pabA"
                     /locus_tag="JQR05_001786"
     CDS             complement(19128..19691)
                     /gene="pabA"
                     /locus_tag="JQR05_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY5323972.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19723..20325)
                     /locus_tag="JQR05_001787"
     CDS             complement(19723..20325)
                     /locus_tag="JQR05_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY5323973.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20315..20482)
                     /locus_tag="JQR05_001788"
     CDS             complement(20315..20482)
                     /locus_tag="JQR05_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY5323974.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20587..21159)
                     /gene="ppiA"
                     /locus_tag="JQR05_001789"
                     /gene_synonym="rotA"
     CDS             complement(20587..21159)
                     /gene="ppiA"
                     /locus_tag="JQR05_001789"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY5323975.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21430..22611
                     /gene="tsgA"
                     /locus_tag="JQR05_001790"
     CDS             21430..22611
                     /gene="tsgA"
                     /locus_tag="JQR05_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY5323976.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSMALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22873..25416
                     /locus_tag="JQR05_001791"
     CDS             22873..25416
                     /locus_tag="JQR05_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY5323977.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25413..25739
                     /gene="nirD"
                     /locus_tag="JQR05_001792"
     CDS             25413..25739
                     /gene="nirD"
                     /locus_tag="JQR05_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY5323978.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25865..26671
                     /gene="nirC"
                     /locus_tag="JQR05_001793"
     CDS             25865..26671
                     /gene="nirC"
                     /locus_tag="JQR05_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY5323979.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26690..28063
                     /gene="cobA"
                     /locus_tag="JQR05_001794"
     CDS             26690..28063
                     /gene="cobA"
                     /locus_tag="JQR05_001794"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY5323980.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLQEAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6829 a         7540 c         7101 g         6684 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcaatgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gccttcggcg ctggtttcac atccagcagc tctacgtcaa acactagggt agaattcggt
     5581 gggatccccg gaacacccgc tttgccgtaa gccagttctg gtggaataac cagtttgatc
     5641 ttaccgcctt tcttgatgtt cttcagacct tctgtccaac ccgggataac accgtccaga
     5701 cggaaagaaa gcggttcacc acgggtgtaa gagttgtcga actctttacc gtcgatcagc
     5761 gtacctttgt agttcactac aacagtatcg ctgtctttcg gtgcttcgcc tttaccggct
     5821 tctactacct gataaaccag accagttgaa gaggttttca cacctttctc tttggcaaat
     5881 ttctcgcggt actctttacc ttttgcttcg ttatcagccg cgtctttttc catcttcgcc
     5941 tgagcagaag acttcacgcg agcttcgaat gcttgcagag tctgttcgat ctcttggtcg
     6001 gagagtttgc tcttatcagc aaatgcatcc tgaacaccag cgatcagctg atctttatcc
     6061 agtttgatgc ccagtttttc ttgttcttta agagagtttt ccatgtaacg acccagcgaa
     6121 gcacccagtg cataagctga tttctgatcg tcatttttga acgctgcttt gctgtcagca
     6181 gttgtagcag gttttgcagc ttcagcagca aaagtgattg gtgcatgcag ggcaacggcc
     6241 attgtggtcg ccagtagcgt tactttaaac agtgatttca tccatatctc caggatcggg
     6301 gcatctcacc ccaaggttaa ctattatcag aagggtacta taaagcgttg tcgaacaaat
     6361 ctacatacag acacgcccta ttatcatcta ttttcagact ctttttgttt aaattagttt
     6421 cgatgaccgc gaaatgagtg ctgtctcggg cagcaaagtt aagtagaatc cgcggcgacc
     6481 attcgacaaa agaggtgaat catgcaggat ttatcattgg aagcacgcct ggcagagctg
     6541 gagagccgac tggcttttca ggaaatcacc attgaagaac tgaacgtcac ggtgaccgct
     6601 catgaaatgg agatggcgaa actgcgcgat catctgcgtc tgctgaccga gaagttaaaa
     6661 gccagccagc cgtcgaacat cgcgtcgcag gctgaagaaa cgccaccgcc acattattga
     6721 ggcgtaaaaa aagcggggat tccccgcttt tttgtcacct tttcggtatt agtggcaacc
     6781 acaaccgccg ttgcctttac cgccacagca gccttcgcca ccgtgttcat gaccgtgatc
     6841 gtggccatga ccgccgcagc aaccgtcgtg gtcgtgatcg tggtgatgat cgtgcgcgcc
     6901 gtgaacgtgg ccatgagcca gttcttcttc ggtcgcttcg cgaatcgcca caacttcaac
     6961 gttgaatttc aggttctgac cggccagcat gtggttacca tcaaccacta cgtgatcgtc
     7021 ttcaaccgca gtgatttcaa ccggtaccgg accctggtca gtttcagcca ggaaacgcat
     7081 acctacctgc agttcatcaa cgcccataaa tacgtcttta ggaacacgtt gcactaggtt
     7141 ttcgtcgtac tgaccgtaag cgtcgttcgc gccaacagcg acatcaaatt tgtcgccaac
     7201 ttcatgacct tccagcgccg tttccaggcc agagatcagg gaaccgtgac catgcaggta
     7261 gtccagcggc gcactcaccg gagactcatc aaccaacaca ccgtcttctg tacgtacctg
     7321 ataggccagg ctgaccacca ggtcttttgc tactttcatg atatctcctg agcatgggaa
     7381 gaatagtggc gcagattgta gcggaattct gcagccgtgt actcactagc ttaaaaaaac
     7441 tcggctcata tcgctagtcc ggatgaaaaa tcccgatcac ttgctcatct ttgcgaacgt
     7501 gatcgcgggc ttctttgtct gcttctcgca tctgatgtcc gcacttaaca cattcaacaa
     7561 tatcaatatt attttcgcgc cacatcgcca ttgaatcctg cgcctgacag gccgggcatt
     7621 ttgcgcccgc tataaaacgt tttcggattg ccatccttac cctctactca aattcatccc
     7681 agccgtccag ctggcgtcgt tcttgttgca tttcacgctg gaaaatctct tccagttcgc
     7741 gtcgggcttc cctggcgcga gaaatttgta cggtatcagc atgcatcggg attaactccc
     7801 gcagcattcg catatccagg cggcgaaaat gcagttgcgc ccgctgtgcc tgatgcggat
     7861 gcatgccaag cgtgaccagc gtcttgcgcc ccagctctaa cgcactggag aatgtttcac
     7921 gggaaaactg cgtcacccct gcctgtaata actcatgcgc ttccacacgt ccgcgcgctc
     7981 gcgcaagaat atgcaaatgc ggaaagtgct gctggcatat ttccaccagc ttcatggtgt
     8041 cttccggctc gttacaggta atgacgatag actcagcggc ctctgcaccc gcagaacgta
     8101 aaagatcgac ctgcgtggcg tcgccgtaat aaactttgta gccatattta cgcatcaggt
     8161 taacggcgct gatatcccgc tccagcacgg taatgcgcat tttatttgcc atcagcaaac
     8221 gaccaatcac ctgaccaaaa cgcccgaagc ccacgacaat gacctgggtt ttatcatcgt
     8281 tgacccacgg tttttcgtct tcttcttccg gtccgttaaa ctggcgggat agccatttat
     8341 ccaccagctt catcagcaac ggcgtggtca tcatggaaag cgtcaccgtc accagcaaca
     8401 gcgccatctg gtcgccctgg aataaacgtt gtgaagaagc ggtagaaaag aggacaaagg
     8461 caaactcacc gccctgactc aacacgccag caaactgcat ccgctctgag ctacgcacgc
     8521 catacaatcg cgccagcaga tacagcacga gaattttcac cgccaccagc acaaccacgc
     8581 ttatcactac ccacaacaga tgggtataaa gcaccccgag gttgagcgac atgccgacag
     8641 agataaagaa caaaccgagc agcaagcctt tgaagggatc gatagccgtt tccagttcat
     8701 ggcgatattc actttccgcc agcagcacgc ccgcaataaa tgtaccgagc gccatcgaca
     8761 gccccagcgc atccataaac aatgcggaac ccaacaccag cagcagcgtc gcggcggtga
     8821 acacttcccg cacgccagaa gctgcaataa agcggaatac cggacgcagt aaatagcgcc
     8881 caccaatcag catgccgaca aacgccagca ccttcatgcc gatcttcatc cagtcgaaat
     8941 gttcgtctgc cgaccccgcc aacaacggca ctaacgccag tgctgggatc actgccagat
     9001 cctgaaacag cagaaccgaa aacccgagct ggccggattc gctgcgattc atccctttct
     9061 cacgcatcaa ttgcaacgcc attgcagttg aagacatcgc aaggccaatg ccaccgacca
     9121 ccgccgcctg ccaggcgaaa tccgtcagca tcaataatcc cgccagcaac gccgcgctta
     9181 acagcacctg tgccgcgcct acgccaaaaa tcgaacgccg cagttgccaa agtttggagg
     9241 gattcaactc aaggccgatg ataaacatca ggaataccac gccgagttcc gaaaagtgga
     9301 ggatctcatc gacgtcgcta ataaacccca gcccccacgg gccaattgca atccctgcca
     9361 gcaaatatcc caacacagcg ccaatcccca gccgcgatgc caacggcacc gcagccaccg
     9421 ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc agcggcctcc
     9481 tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg ccagctcctt
     9541 tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca tccggcacat
     9601 gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat tcagcgcgtc
     9661 ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt actttcccgc
     9721 cagttggttt cctcccggcc cactggcaaa accacgactt aatacccggt ccagccactc
     9781 tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa tcacctcgtg
     9841 ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat agtgcgcgta
     9901 aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta cccggtttgc
     9961 caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg gctgagacat
    10021 catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc attgcgcccg
    10081 gtgcggcagc tcgcccatac attacattat cataatgata agttaacata gtctgaacat
    10141 acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc gcgtcctgct
    10201 ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg gtaaaaacgg
    10261 ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg acggcggcag
    10321 ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc cagcattacc
    10381 gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac tggaagcgca
    10441 gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc atagcaagct
    10501 ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc acggcctcgg
    10561 tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct ggcgtatgcg
    10621 tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg acgaaccgac
    10681 taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga gctatcaggg
    10741 cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg ataaaattat
    10801 tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt ttgaagtaca
    10861 gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg aacgcgtggc
    10921 acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga agcaggccca
    10981 gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg tcgacaaccc
    11041 gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga agatggaaaa
    11101 ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga atctggtccc
    11161 cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat taatcaaact
    11221 gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag ggatcaagct
    11281 cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac ctattcaaca
    11341 tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc tcggcggctt
    11401 tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg gggaaaaagc
    11461 ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc tcgacgaacc
    11521 gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa tcgagtttga
    11581 aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca ctgacgatct
    11641 ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag attatcaaca
    11701 gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa aagagaacgc
    11761 gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc gtgcgcaaac
    11821 ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc tgaacgcgca
    11881 actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga gccgtaaagc
    11941 ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg aagagtgcga
    12001 aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag gccaaagcaa
    12061 ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc tgaattcacc
    12121 cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt gtttcgtcgt
    12181 caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga ttttgtcgat
    12241 ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt ggtgtttcac
    12301 gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc ggcgcaaaag
    12361 cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc aaaccgtatg
    12421 caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg ctggctgcaa
    12481 cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg taatatgctg
    12541 gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt gattgtctct
    12601 gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc ccgcgtttat
    12661 cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc agcctacccc
    12721 ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg tgaatttgac
    12781 gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta tcgtcagtgt
    12841 agcgccatgc cgatgctgaa ccggatcgcc aaaccgacgc tgattattca cgccaaagac
    12901 gatccgttta tggatcatca ggtgatcccg aaaccggaaa gtctcccctc gcaggtggag
    12961 tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt acttcatccg
    13021 caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga ggcgaaatca
    13081 tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt gaaagctttg
    13141 tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag aaagtcgccg
    13201 acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag ctgcacgaaa
    13261 cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc ggcggacgag
    13321 ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg tcattgcggt
    13381 aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta aaatattcgc
    13441 gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt acacccgccc
    13501 ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca gctacttcgg
    13561 ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg gtcagagcgg
    13621 caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct ggaatcaggt
    13681 accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac tgttttatga
    13741 aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg tggacttact
    13801 ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc gcgacaccag
    13861 cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa tggaagacta
    13921 tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc gcgttcccac
    13981 cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg aaagctttgt
    14041 ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg cgatgttgca
    14101 aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa tggggctggc
    14161 aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga aaatcgagta
    14221 actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag ttaagtcacg
    14281 cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc aacgccactg
    14341 aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct ttcaggtcgc
    14401 gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc tcttcgcggc
    14461 gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt tgcaggatcg
    14521 aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc atcggacttg
    14581 gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg gattcgccca
    14641 gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact ccactgcgtc
    14701 aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg acctgggtca
    14761 ggctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag tcaggatgct
    14821 acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg tgcagtacag
    14881 ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga cagcggcgtt
    14941 atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt ggcaaaccgc
    15001 aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag tacccatcca
    15061 agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc gttaaaggct
    15121 ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc tatctgaatc
    15181 agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt agcgcatggg
    15241 tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt cgccaattga
    15301 ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt cgtctgcaag
    15361 tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc attgcacaga
    15421 ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt atgcaaatca
    15481 aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc gtgggacgca
    15541 ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc atcgtcgttt
    15601 acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca ttttgtttcc
    15661 cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc gacaaacgct
    15721 ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat tcggtctgct
    15781 cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc ataaacgttt
    15841 tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac tgacacagct
    15901 gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc tggtacttgg
    15961 cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc tgctcgccgc
    16021 catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc tcatctatgc
    16081 gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct ggcgcgaaca
    16141 accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt gtgaagccaa
    16201 atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc tgctggtgcg
    16261 ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata tgctttccgc
    16321 gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc tggatttgca
    16381 ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg tcgagcgtag
    16441 ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc tgcgcgtgct
    16501 ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa agcaaattgg
    16561 cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat tctgctactg
    16621 gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg cccgtgactt
    16681 actggccgat aaacagcggc gaatgccgtt acttccggca ctgaaaagtt atctgtcact
    16741 aaagtctccg gcgctacgca atgccggacg actcagtgtg atattaagcg ttgccagcct
    16801 gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg tattgctggt
    16861 gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg tgggaaccgt
    16921 ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaag gttacaccct
    16981 gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa actacggctg
    17041 ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt ggctgaacgg
    17101 tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga ttgctttcgg
    17161 tggtaccgtc tggctatggc cgcagtggca gagcgggtta ttgcgtaaaa acgcccatga
    17221 tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc cgcaaccaac
    17281 gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt ataactcatt
    17341 gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata tgaaactgtg
    17401 ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc tggcgcggga
    17461 acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg aaatcgccat
    17521 tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg atgccaatat
    17581 catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg agcagcattt
    17641 acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg catggcgtca
    17701 gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg cgtaatacag
    17761 gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg atgcgtctta
    17821 tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct tcatcgatat
    17881 ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg gcattcagta
    17941 ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct ttgtagtgtg
    18001 gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac acatcgtact
    18061 gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga atgccttcca
    18121 gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag gccagaggat
    18181 taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt tccgccgtgg
    18241 tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc agaatatccg
    18301 gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc ccgcactgca
    18361 cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag ccctgcaaaa
    18421 actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc accaccgcac
    18481 aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga acgtggatga
    18541 tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc accgaaacgg
    18601 taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt ttgaacgggc
    18661 tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta gcttccgtgc
    18721 cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg cgtccaagac
    18781 gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg ccctgggttt
    18841 ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca atgccacccg
    18901 cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga ccttttaccg
    18961 gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc gcgcgtgtaa
    19021 ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata aatgtgaaat
    19081 tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca gagatgcagg
    19141 aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg gaactgcaca
    19201 ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc gctccaggcc
    19261 gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg gtagcgtgtc
    19321 acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt aatcggcgag
    19381 gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc ctgcgccatt
    19441 gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg gcgaataacg
    19501 tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac aattttttgt
    19561 ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac cagcacatcc
    19621 gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta gttatctata
    19681 agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag attccccggc
    19741 ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca ttgccccact
    19801 ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt gatatccggc
    19861 atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca cccggaaggg
    19921 atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact tcgctttctc
    19981 caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc cctctttttc
    20041 gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt cacgcagttg
    20101 ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca aatgcggtaa
    20161 accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca tttcgtaagc
    20221 ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga tatcaaggcc
    20281 aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc ctcctcagct
    20341 cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc tcgacgcctt
    20401 caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga cgggactttt
    20461 gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg ccacaagtat
    20521 aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg cccgcgcgag
    20581 aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg gcacattctg
    20641 gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt ccatgccttt
    20701 caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa ggaaggcgtt
    20761 atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg tacgcgccat
    20821 cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga ttggcgggtt
    20881 tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa agccaggaat
    20941 gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga caaagttttg
    21001 cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag ctgaggttgt
    21061 caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag cagaaagagc
    21121 gaaaacagca gccatcgccg ccagggtcga tttgaacatg agatttcctt aacaaacaga
    21181 gcagaaaaaa agcaagtgaa gagattctaa atagccttca agaacaaggc catcccttta
    21241 cctaatttta cgtatctgaa atatatgtaa cctgacgatc actttttatt ccgtgatcaa
    21301 aatcacctct taaaatgcaa tttagcaacc gattgcaata aaacatttaa acagatcaca
    21361 aaatcaccta aaatcgcccg ctcgcggcat atcccgctat gccattttat ttttgaacgc
    21421 aggccaatca tgactaacag caatcgcatc aagctcacat ggattagctt tctctcctac
    21481 gcactgactg gtgcgttggt tattgtcacc gggatggtga tgggaaatat cgccgattat
    21541 ttcaatctgc ctgtttccag tatgagtaac accttcacct tcctcaacgc cggcatttta
    21601 atctctatct tcctcaacgc ctggctgatg gaaatcgtcc cgttgaaaac gcagttacgt
    21661 tttggctttc tcctgatggt gctggcggtt gccggtttga tgttcagcca cagcatggcg
    21721 ctgttctcga cggcgatgtt cattctcggg gtggtcagcg gcattactat gtcgattggt
    21781 acattcctga taacacaaat gtatgaaggg cgtcagcgcg gttcccgcct gttatttact
    21841 gactccttct tcagtatggc tgggatgatt ttcccaatga tcgccgcgtt tctactggcg
    21901 cgcagcattg agtggtactg ggtttatgcc tgcatcgggc tggtgtatgt cgctattttt
    21961 attctgacct tcggctgtga gttcccggca ctgggcaaac gtgcaccaaa aacggatgct
    22021 ccggtagaga aagaaaagtg ggggatcggc gtactgtttc tctctgttgc ggcgctgtgc
    22081 tacatcctcg gtcagttagg ttttatctcc tgggtgcctg agtatgccaa aggcctgggc
    22141 atgagcctga acgacgcggg cacgctggtg agtaacttct ggatgtcata catggtcggc
    22201 atgtgggcgt tcagctttat tcttcgcttc tttgatttgc aacgcattct gaccgtactg
    22261 gctggtctgg ctgcgattct gatgtacgtc tttaacaccg gaacaccagc acatatggcg
    22321 tggtcaattc tcgctctggg cttcttctcc agcgcgatct ataccaccat catcaccctg
    22381 ggttcacagc agaccaaagt accgtcgcca aaactggtta actttgtcct gacctgcgga
    22441 accatcggta ctatgttgac ctttgtggtt accggcccga ttgttgaaca tagcggtccg
    22501 caggcggcac tgcttacagc aaacggtctg tacgctgtcg tctttgtgat gtgcttcctg
    22561 ttaggtttcg tcagccgtca ccgtcagcat aacaccctga cctctcatta attgctaatg
    22621 ccggacggca ctatcgtcgt ccggcctttt cctctcttcc cccgctacgt gcatctattt
    22681 ctataaaccc gttcattttg tccatttttt gcacaaacat gaaatgtcag acaattccgt
    22741 gacttaagaa aatttataca aatcagcaat atacccctta aggagtatat aaaggtgaat
    22801 ttgatttaca tcaataagcg gggttgctga atcgttaagg taggcggtaa tagaaaagaa
    22861 atcgaggcaa aaatgagcaa agtcagactc gcaattatcg gtaacggtat ggtcggccat
    22921 cgctttatcg aagatcttct tgataaatct gatgcggcca actttgatat taccgttttc
    22981 tgtgaagaac cgcgcatcgc ttatgaccgc gtacacctct cgtcttactt ctctcaccac
    23041 accgccgaag agctgtcgct ggtgcgcgaa ggcttctacg agaaacacgg catcaaagtt
    23101 ctggtcggcg aacgcgctat caccatcaac cgtcaggaga aggtgattca ctccagcgcc
    23161 ggacgtaccg ttttttatga caagctgatc atggcaaccg gttcctaccc gtggatccca
    23221 ccaatcaaag gttctgatac tcaggactgc tttgtctatc gcaccattga agacctcaac
    23281 gccattgaat cctgcgcccg tcgcagcaaa cgcggtgccg ttgttggtgg cggcctgtta
    23341 ggtctggaag ccgctggcgc gctgaaaaac ttaggtattg aaacccacgt tatcgaattt
    23401 gcccctatgc tgatggcgga acagcttgat cagatgggtg gcgagcagct gcgtcgcaaa
    23461 atcgaaagta tgggcgtgcg cgttcacacc agcaaaaaca cccttgagat tgtgcaggaa
    23521 ggtgtcgaag cgcgtaaaac catgcgtttt gccgacggca gcgaactgga agtcgacttt
    23581 atcgtcttct ctaccggtat ccgtccgcgc gataagctgg caacccagtg tggtctggac
    23641 gttgctccgc gtggggggat tgtcattaat gattcctgcc agacttccga tccggatatc
    23701 tacgccatcg gtgaatgcgc aagctggaac aaccgtgtat ttggtctggt agcaccaggc
    23761 tacaaaatgg cgcaggtcgc cgttgaccat attctgggta gcgaaaacgc ctttgaaggt
    23821 gctgacctta gcgccaagct gaaactgctg ggcgtagacg taggtggtat tggtgatgcg
    23881 cacggtcgca cgcctggcgc acgtagctat gtttacctcg acgaaagtaa agagatctac
    23941 aaacgcctga ttgtcagcga agacaacaaa accctgctcg gtgcggtact ggtgggcgat
    24001 accagcgact acggcaacct gctgcaactg gtgctgaacg ctatcgagct gccggaaaac
    24061 ccggattctc tgattctgcc agcgcactcg ggtagcggta agccgtctat cggtgttgat
    24121 aaactgccgg acagcgcgca aatctgctcc tgcttcgacg tcactaaagg tgatctgatt
    24181 gctgccatca acaaaggctg ccacacagtt gcggcgctga aagctgaaac caaagcgggt
    24241 actggctgcg gtggctgtat cccgctggtc actcaggtac tgaacgcgga actggcgaaa
    24301 cagggcatcg aagttaacaa caacctgtgc gaacactttg cttattcgcg tcaggaactg
    24361 ttccatttga tccgcgttga aggcattaaa accttcgaag aactgctggc gaaacacggc
    24421 aaaggctacg gttgtgaagt ttgtaaacca accgtcggtt cgctgctggc ctcctgctgg
    24481 aacgaataca ttctgaagcc ggaacatact ccgctgcagg attctaacga caacttcctc
    24541 gctaacatcc agaaagacgg cacctactcg gtgatcccgc gttctccggg cggtgaaatc
    24601 accccggaag ggctgatggc agtgggtcgt atcgcgcgtg aatttaatct ctacaccaaa
    24661 atcaccggct cccagcgcct ggcgatgttt ggcgcacaga aagacgatct gccggaaatc
    24721 tggcgtcagc tgattgaagc tggcttcgaa accggtcatg cctatgcgaa agcactgcgt
    24781 atggcgaaaa cctgcgtggg tagcacctgg tgccgctacg gtgttggcga tagcgtcggc
    24841 ctcggcgtgg aactggaaaa ccgctacaaa ggcatccgta cgccgcacaa aatgaagttc
    24901 ggtgtctccg gctgtacccg tgaatgttca gaagctcagg gtaaagacgt gggtattatc
    24961 gccactgaaa aaggctggaa cctgtatgtt tgcggtaacg gcggcatgaa accgcgtcat
    25021 gcggatctgc tggcagcgga tatcgatcgc gaaacgctga tcaaatatct cgaccgcttc
    25081 atgatgttct acatccgtac cgccgacaaa ctgacgcgta ccgcgccgtg gttagaaaac
    25141 ctcgaaggcg gcatcgatta cctgaaagcg gtgatcattg acgacaaact ggggctgaac
    25201 gctcatctgg aagaagagat ggcgcgcctg cgtgaagcgg tagtgtgtga gtggactgaa
    25261 acggtcaata caccgtccgc gcagactcgc ttcaaacact tcatcaacag cgacaagcgt
    25321 gacccgaacg tgcagatggt gccagagcgc gaacagcacc gtccggcaac gccgtatgaa
    25381 cgtatcccgg taactctggt ggaggacaac gcatgagcca gtggaaagac atctgcaaaa
    25441 tcgatgacat cctgcctgaa accggcgtct gcgcgctgtt aggtgacgag caggtagcga
    25501 ttttccgccc gtatcacagc gaccaggtgt ttgcgatcag caacattgat ccgttcttcg
    25561 agtccagcgt gctatcacgc gggctgattg cggaacacca gggcgagctg tgggtcgcca
    25621 gcccactgaa aaaacagcgt tttcgcttaa gtgatggctt gtgcatggaa gacgaacagt
    25681 tttccgtcaa acattacgaa gcacgagtga aagacggcgt ggtgcagctg cgcggttaat
    25741 gttttaacgg gaggcgcaat gcctcccctt tttgcatggt cctgtaatga ccttcggtat
    25801 attgcaggac attttttaaa ctttttgttt tattttttgt ttttattttt taaaggataa
    25861 tcaaatgttt acagacacta ttaataagtg tgcggctaat gctgcgcgca ttgcacgcct
    25921 gtcggcaaat aacccgctcg gcttttgggt cagctccgcc atggcgggcg catatgtggg
    25981 tcttgggatc atcctgattt tcacgctcgg taatttgctc gacccgtccg tacgtccgct
    26041 ggtgatgggc gcgacctttg gtatcgcctt aacgctggtg attatcgccg gttctgaact
    26101 gttcaccgga cacaccatgt tcctcacctt tggggtaaaa gcgggcagca tcagccacgg
    26161 gcaaatgtgg gcaatcctgc cgcaaacctg gctgggtaac ctggtcggtt ccgtcttcgt
    26221 tgccatgctc tatagctggg gcggcggtag cctgctgccg gtagatacca gcatcgttca
    26281 ctccgtcgcg ctggctaaaa ccactgcacc ggcaatggta ctgttcttca aaggtgcatt
    26341 gtgtaactgg ctggtttgcc tggcaatctg gatggcgctg cgcactgaag gggcggcgaa
    26401 atttatcgct atctggtggt gtctgctggc atttatcgcg tccggctacg agcactctat
    26461 cgctaacatg acgctgttcg cgctctcctg gttcggcaac cacagcgaag cctacacgct
    26521 ggcgggtatt ggtcataacc tgctgtgggt gacgctgggt aatactttat caggtgccgt
    26581 attcatggga ttgggttatt ggtatgctac gccgaaagcg aatcgtccgg ttgcggacaa
    26641 atttaatcaa actgaaacgg ctgccggtta attactaagg ggtttttacg tggatcattt
    26701 gcctatattt tgccaattac gcgatcgcga ctgtctgatt gtcggcggtg gtgatgtcgc
    26761 ggaacgcaaa gcaaggttgc tgttagacgc aggcgctcgc ttaacggtga atgcattagc
    26821 gtttattcca cagttcaccg catgggcaga tgcaggcatg ttaaccctcg tcgaagggcc
    26881 atttgatgaa agccttctcg acacctgctg gctggcgatt gcagcgacgg atgatgacac
    26941 gcttaaccag cgcgtcagcg aagccgctga agctcgtcgc atcttctgta acgtagtcga
    27001 tgcgccgaaa gccgccagct ttattatgcc gtcgattatt gaccgctcac cgctaatggt
    27061 agcggtctcc tctggcggca cctctccggt tctggcgcgc ctgttgcgcg aaaaactcga
    27121 atcactgctg ccgttgcatc tgggccaggt ggcgaaatat gccgggcaat tacgcgggcg
    27181 agtgaaacaa cagttcgcca cgatgagtga gcgtcgccgt ttctgggaga aattgttcgt
    27241 taacgaccgc ctggcgcagt cgctggcaaa caacgatcag aaagccatta ctgaaacgac
    27301 cgaacagtta atcaacgaac cgctcgacca tcgcggtgaa gtggtgctgg ttggtgcagg
    27361 tccgggcgat gccgggctgc tgacgctgaa aggactgcaa caaattcagc aggcagatgt
    27421 ggtggtctac gaccgtctgg tttctgacga tattatgaat ctggtacgcc gcgatgctga
    27481 tcgcgttttc gtcggcaaac gcgcgggata ccactgtgta ccgcaggaag agattaacca
    27541 gatcctgctg caggaagcgc aaaaaggcaa acgcgtggtg cggctgaaag gtggcgatcc
    27601 gtttattttt ggccgtggtg gcgaagagct ggaaacactg tgcaacgcgg gtattccgtt
    27661 ctcggtggtt ccgggtatta ccgcagcttc tggttgctct gcctattcgg gtattccact
    27721 cacgcatcgc gattatgccc agagcgtacg cttaattacc ggacacttaa aaaccggtgg
    27781 cgagctggac tgggaaaacc tggcggcaga aaaacagacg ctggtgttct atatggggtt
    27841 gaatcaggcc gcgactattc agcaaaagct gattgaacac ggtatgcctg gcgaaatgcc
    27901 ggtggcaatt gtcgaaaacg gaacggcagt cacgcagcgc gtgattgacg gtacgctcac
    27961 gcaactcggt gaacttgctc agcaaatgaa cagtccatcg ctaattatta ttggtcgggt
    28021 tgttggcctg cgcgataaac tgaactggtt ctccaatcat taatttaacc cgggccagag
    28081 aattctggcc ttcttaatga ctctttttat atattcagca aataaaacat aaccccttaa
    28141 taaatattat ctgc
//
LOCUS       DADKXM010000043        27563 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.043, whole genome shotgun sequence.
ACCESSION   DADKXM010000043 DADKXM010000000
VERSION     DADKXM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.043"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..796
                     /locus_tag="JQR05_001795"
     CDS             <1..796
                     /locus_tag="JQR05_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5323981.1"
                     /translation="WLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1784
                     /locus_tag="JQR05_001796"
                     /pseudo
     CDS             807..1784
                     /locus_tag="JQR05_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JQR05_001797"
     CDS             complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JQR05_001797"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY5323982.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2752..4788
                     /locus_tag="JQR05_001798"
     CDS             2752..4788
                     /locus_tag="JQR05_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY5323983.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4746..5141
                     /locus_tag="JQR05_001799"
     CDS             4746..5141
                     /locus_tag="JQR05_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY5323984.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5161..5751
                     /gene="diaA"
                     /locus_tag="JQR05_001800"
     CDS             5161..5751
                     /gene="diaA"
                     /locus_tag="JQR05_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY5323985.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5761..6336
                     /gene="yraP"
                     /locus_tag="JQR05_001801"
     CDS             5761..6336
                     /gene="yraP"
                     /locus_tag="JQR05_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY5323986.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6450..7490)
                     /locus_tag="JQR05_001802"
     CDS             complement(6450..7490)
                     /locus_tag="JQR05_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5323987.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVTMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7563..8198)
                     /locus_tag="JQR05_001803"
     CDS             complement(7563..8198)
                     /locus_tag="JQR05_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY5323988.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8326..8844
                     /gene="yhbO"
                     /locus_tag="JQR05_001804"
     CDS             8326..8844
                     /gene="yhbO"
                     /locus_tag="JQR05_001804"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY5323989.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLATSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8824..9267)
                     /locus_tag="JQR05_001805"
     CDS             complement(8824..9267)
                     /locus_tag="JQR05_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY5323990.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9318..9620
                     /gene="yhbQ"
                     /locus_tag="JQR05_001806"
     CDS             9318..9620
                     /gene="yhbQ"
                     /locus_tag="JQR05_001806"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY5323991.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9607..10110)
                     /locus_tag="JQR05_001807"
     CDS             complement(9607..10110)
                     /locus_tag="JQR05_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5323992.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10104..10628)
                     /locus_tag="JQR05_001808"
     CDS             complement(10104..10628)
                     /locus_tag="JQR05_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY5323993.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGMYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10837..11832
                     /locus_tag="JQR05_001809"
     CDS             10837..11832
                     /locus_tag="JQR05_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY5323994.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDDYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11841..12719
                     /locus_tag="JQR05_001810"
     CDS             11841..12719
                     /locus_tag="JQR05_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY5323995.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12800..13807
                     /locus_tag="JQR05_001811"
     CDS             12800..13807
                     /locus_tag="JQR05_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY5323996.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13925..15169)
                     /gene="mtr"
                     /locus_tag="JQR05_001812"
     CDS             complement(13925..15169)
                     /gene="mtr"
                     /locus_tag="JQR05_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY5323997.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15323..17212)
                     /locus_tag="JQR05_001813"
     CDS             complement(15323..17212)
                     /locus_tag="JQR05_001813"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY5323998.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17205..17285)
                     /gene="yrbN"
                     /locus_tag="JQR05_001814"
     CDS             complement(17205..17285)
                     /gene="yrbN"
                     /locus_tag="JQR05_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY5323999.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17392..18276)
                     /gene="nlpI"
                     /locus_tag="JQR05_001815"
     CDS             complement(17392..18276)
                     /gene="nlpI"
                     /locus_tag="JQR05_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY5324000.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18385..20520)
                     /gene="pnp"
                     /locus_tag="JQR05_001816"
     CDS             complement(18385..20520)
                     /gene="pnp"
                     /locus_tag="JQR05_001816"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY5324001.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNAVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGCV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20767..21036)
                     /gene="rpsO"
                     /locus_tag="JQR05_001817"
     CDS             complement(20767..21036)
                     /gene="rpsO"
                     /locus_tag="JQR05_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY5324002.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21185..22129)
                     /gene="truB"
                     /locus_tag="JQR05_001818"
     CDS             complement(21185..22129)
                     /gene="truB"
                     /locus_tag="JQR05_001818"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY5324003.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYACQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LCELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22129..22530)
                     /gene="rbfA"
                     /locus_tag="JQR05_001819"
     CDS             complement(22129..22530)
                     /gene="rbfA"
                     /locus_tag="JQR05_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY5324004.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22694..25366)
                     /gene="infB"
                     /locus_tag="JQR05_001820"
     CDS             complement(22694..25366)
                     /gene="infB"
                     /locus_tag="JQR05_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY5324005.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25391..26878)
                     /gene="nusA"
                     /locus_tag="JQR05_001821"
     CDS             complement(25391..26878)
                     /gene="nusA"
                     /locus_tag="JQR05_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY5324006.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26906..27358)
                     /gene="rimP"
                     /locus_tag="JQR05_001822"
     CDS             complement(26906..27358)
                     /gene="rimP"
                     /locus_tag="JQR05_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY5324007.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6571 a         7629 c         6913 g         6450 t
ORIGIN      
        1 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcaa cgctaagtag cactaactgg caatacgcct
      901 gttcctgctc tgcgggtaag gcagttaaac ttgtctatat ggtcagcccc gtacttacca
      961 ccactggaca tcagacagga tattacaaac tcaatgacag cctagatatt aaaaccacat
     1021 tacaggcaaa cgacattcca ggactcacaa ccgaccaggt tgtctctgtt aacacccgat
     1081 tcacacagat aaaaagcaac accgtatatt ctgctgcaac ccaaacgggt gtttgccagg
     1141 gtgacacgtc tcgttatgga cccgttaata ttggtgcaaa taccaccttt accctgtatg
     1201 tcaccaagcc atttctcggc tcgatgacca ttccgaaaac ggatattgcc gtcattaaag
     1261 gcgcgtgggt cgatggaatg ggaagcccgt ctacaggtga cttccatgat ttagtcaagt
     1321 tatcgattca gggaaatctc accgccccac agtcgtgcaa aattaatcag ggcgatgtta
     1381 ttaaagttaa ttttggattc atcaatggtc agaagtttac cacccgcaat gccatgccag
     1441 acggttttac tccagtagac tttgatatca cttatgactg tggtgatact tcaaagatta
     1501 aaaactcgtt gcaaatgcgc attgacggta caactggggt agtagaccag tacaacctgg
     1561 tcgccaggcg aagaagttca gacaatgtgc ccaatgtcgg tattcgtatt gaaaatctcg
     1621 gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat ccttccgggc
     1681 atggcaccgt caatatgcgc gcctagccag ttaatctggt cggtggtgag ctggaaacag
     1741 gaaaatttca gggcacagcc accattaccg tcatggtgcg gtaaaagaag acgttgcgtt
     1801 ttgattaacg caacgcttcg gcactgttac ccctgctgct ccagcgcata cttatacagc
     1861 gcatttttct tcacgccatg aatttctgcg gccagcgccg ccgctttttt cagcggcagt
     1921 tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct
     1981 ttatgacctt cgacaatcag caccatctcg cctttgcgac ggttttcatc ttcctttacc
     2041 cacgccagca gctcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca
     2101 cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct
     2161 aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt
     2221 agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca
     2281 gatggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg tagcggcacc
     2341 acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc
     2401 ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc
     2461 gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca
     2521 aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc
     2581 tgtaatacct ctaacgtacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca
     2641 atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta
     2701 ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc
     2761 tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt
     2821 ttcgccggtt gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg
     2881 caggctgatt ctgcctttta tcttcagcag atgcagcaaa gctctgatga taccaggatc
     2941 aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt
     3001 gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga gaaaacactg
     3061 ctggcggcag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg
     3121 aaaatcacac ctgccgattt agaacaaaac cagcaagcgc gttactggca ggcaaaaatc
     3181 gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg
     3241 ctgcttggcg cgaaagaaaa acagcagaat attgatgcca cctggcaggc gctctcctcc
     3301 atgactcagg aacaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc
     3361 tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg atcccgacat gatgaaagcc
     3421 gggatcgccg actggcagaa acgttatccg aacaatccgg gcgcgaaaat gctgccaacg
     3481 cagttggtta acgtaaaagc gtttaaacca gcctcgacca acaaaatcgc cctgctgttg
     3541 ccgctgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagccgcg
     3601 aaaaatatcg gcactcagcc agtggcggct caggtagctg ccgcacctgc cgcagacgta
     3661 gcagaacaac ctcagccgca aactgcggat ggcgttgcca gcccggcaca agcctcggtt
     3721 agcgatctga ccggtgatca gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca
     3781 agcaccgcag cggtaagcgc acccgcaaat ccatccgcag agctgaaaat ctacgatacc
     3841 tcatcacaac cacttagcca gatcttaagc caggttcagc aggatggcgc gagtattgtg
     3901 gttggtccgt tgctgaaaaa taacgttgaa gagttgctga agagcaacac cccgctgaac
     3961 gtactggcac tgaaccagcc ggagaatatc gaaaaccgcg tcaatatttg ttacttcgcg
     4021 ctttcaccgg aagacgaagc gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa
     4081 gcgccgctgg tgctgatccc acgcagtgca ttgggcgatc gcgtagccaa tgcgtttgcg
     4141 caagagtggc agaaactggg cggcggcacc gttctgcaac aaaaatttgg ttccaccagc
     4201 gaattacgcg cgggtgttaa cggcggttct ggtattgctt taacgggtag cccgattact
     4261 cccagagcga caaccgactc cggcatgacg accaacaatc caacgctgca aaccacgcca
     4321 accgatgacc agttcaccaa taatggcggt cgtgtcgatg cggtctacat tgtggcaacg
     4381 ccgggtgaaa tcgcttttat caaaccgatg atcgccatgc gtaacggtag ccagagcggt
     4441 gcaacgctgt acgccagttc ccgcagcgca caaggcaccg caggcccgga tttccgactg
     4501 gagatggaag gcttgcagta cagcgaaatc ccgatgctgg caggcggtaa tctgccgtta
     4561 atgcagcagg cgctcagcgc ggtgaacaac gattattcgc tggctcgcat gtatgcgatg
     4621 ggcgttgatg cctggtcgtt ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt
     4681 gaaatcaacg gtaataccgg aagtctgacg gctaacccgg attgcgtgat taacaggaag
     4741 ttatcatggc tacagtacca acaaggtcag gtagtccccg ccagttaacc accaaacaga
     4801 ccggcgatgc gtgggaagca caagcgcgtc gctggctgga aggcaaagga ctgcggttta
     4861 tcgccgctaa cgtgaacgag cgtggcggcg agatcgatct gataatgcgt gaaggcctga
     4921 ccaccgtttt tgtcgaggta cgctatcgcc gctctgcgct ttatggcggc gcggcagcca
     4981 gtgtgacccg cagcaaacaa cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc
     5041 ataatgggag ttttgatact gtggattgcc ggttcgatgt ggtagccttc accgggaatg
     5101 aggttgagtg gattaaggat gcctttaatg accactcata attaaggttt aaggattagc
     5161 gtgcaagaaa gaattaaagc ttgcttcact gaaagcattc aaactcaaat tgcggcggca
     5221 gaggcgcttc cggatgccat ctcccgtgca gccatgacgc tggttcagtc tctgctcaat
     5281 ggcaacaaaa tcctctgttg tggtaatgga acttccgctg ccaatgcaca gcattttgct
     5341 gccagcatga tcaaccgttt cgaaacggag cggcccagct tacctgccat tgcactaaat
     5401 actgataatg ttgtcttaac ggcgattgcc aacgatcgct tacatgatga agtgtatgca
     5461 aaacaggtgc gggcgctggg tcatgcggga gatgtattgt tagccatttc cacccgtggc
     5521 aacagccgcg atattgttaa agcagttgaa gccgccgtta cgcgtgatat gaccattgtg
     5581 gcattgaccg gctatgacgg cggcgaactt gcaggtttgt tagggccaca ggatgtggag
     5641 atccgcattc cttcgcatcg tagtgctcgc attcaggaaa tgcatatgct gacggtaaat
     5701 tgcctgtgcg atctgatcga taacacgctt ttccctcacc aggatgatta aggagaatac
     5761 atgaaggcat tatcgccaat cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt
     5821 gccgctgccg tagtgggtac cgctgctgtg ggtaccaaag ccgcaactga cccacgcagt
     5881 gtcggcaccc aggtggacga tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa
     5941 gacgaacaga ttaagaaaga aacccgtatt aatgtcacgg cctatcaggg caaagtgctg
     6001 ctggttgggc agtcaccaaa tgctgaactt tcggctcgcg ccaaacagat tgctatgggc
     6061 gtagacggtg ccaacgaagt gtataacgag attcgtcagg gccagccgat tggtctgggc
     6121 gaagcatcta acgatacgtg gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac
     6181 ctggtgaaat cgtccaacgt gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg
     6241 ctggtgactg aacgtgaagc gaaagcggcg gcagatattg ccagccgggt gagcggcgta
     6301 aagcgggtaa ccacagcgtt tacgtttatt aaatagcagc cccttgtaat gcctgatgcg
     6361 acgcttgccg cgtcttatca ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt
     6421 acgccgcatc cgacatgttt tccctcaaat cagaacaaca gcgccagccc gccgacaatc
     6481 acaccagaca ctgccaccat cgcccctgtc agccataagg ctttcgccgg gaacgctttg
     6541 cgcagcataa tcagtgacgg caaactcact gccgggagcg tcatcaacaa tgccagcgcc
     6601 ggagcggttc ccatacctgc cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg
     6661 ggaatgacaa acaagcatcc tgctaccgcc atcgtcacca cccacatcag gctgttatcg
     6721 acagcaccat cggcatgggg gaataaccag acgcgtgcgg cacccaacac cagtactgca
     6781 aggatgtaaa ccgggatcgt actccagaaa agtgtccata gcgccctgcc ccagcggcta
     6841 aaaaaaccgc tctgtgcttc cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt
     6901 tcacgcaccc atttttgcac cagcgtcgca atcagcaaca ccatcaccag cccggccacc
     6961 agacgaatcg ccgcaaaacc ccagccgagg acaaacccca taaacaccag cgtcgccggg
     7021 tttaacaccg gattgcccat ccagaatgcc agcgcaccgc ccatcgatac ctgttggcga
     7081 cgcattcccg ccgcgaccgg agccgcacag caggtacaca tcatgcccgg cagcgaaaac
     7141 agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa gcgtacgcaa caaccagtca
     7201 cgcgggatca gcacctgaat caacgaaccg aggatcaccc ccagcaccgc cgctttccat
     7261 actgcgagga aatagatcat cgcgtaatcc aacgccgctt gccacggatt agcatccgcc
     7321 tgcgcaagga tagatttacc gatactgtgg gtttcggctg cagtaaacgc tttgccgtag
     7381 taaggttgcc atttcacata ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt
     7441 ttccaccact gaatgggcgt tgccgcctga gatgaagact gaccagtcat agcattcccc
     7501 aggaataagt tatgataatt gagcgcgtga atattacgct cactatcaat tcttgggaat
     7561 aattattcag ctcttttgcg taattctgaa gagcttaaaa tcgtcacgcc ttcatgctca
     7621 ggccgcatcg cctctgccag catgacccgc gccacgtctc tcgcatcaat ggatttccag
     7681 ttacctggta acaaacggaa cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta
     7741 cgatcgccca gtaacatcga cgggcgagca atggtcaatt tcggccagtt ctgggcaatt
     7801 aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa acggcgagtg ggcattggca
     7861 cccatagcac tgaccaccag catatgctgc gcgcccagtc gccgcccggt taatgcggta
     7921 tccactacca gcgtgtaatc ggcatgaata aacgcctctt tgctccccgc ttctcgccgc
     7981 gtggtgccga gacaacaaaa cacaatgtcg atgggttcgg tgacctgcgc caacgcgtca
     8041 ctcagttgcg gatcatgggg attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc
     8101 ggcgcagcaa tggcgttaac tttcggttca ttaatcaaca tccgcagcag gtgaccgccc
     8161 accagccccg ttgcgcctgt aatcagtacc tgactcatct tcgctccttt acagaattgt
     8221 ctgccttgcg ctccccggct catgcaccag tcttaataga ccatgaggta attatccccg
     8281 attgtggaaa attcgcctca tccaatgcaa caacgcggag gaagcatgag taagaaaatt
     8341 gccgttttaa tcactgatga atttgaagat tcagaattta cttcacccgc agacgagttc
     8401 cgtaaagccg gacacgaagt gattactatt gaaaaacaag cgggtaaaac ggtgaaaggc
     8461 aaaaaaggag aagccagcgt gaccatcgat aaatccatcg atgaagtgac gccagcggag
     8521 tttgatgccc tgttgctacc gggtggtcat tcaccggatt atctgcgtgg cgacaaccgt
     8581 tttgtcacct ttacccgtga ttttgtgaat agcggcaaac ctgttttcgc catctgtcac
     8641 ggtccgcagc tgctgatcag cgccgatgtg attcgggggc gcaaactgac cgcagttaaa
     8701 ccgatcatta ttgatgttaa aaatgcgggc gtggaatttt acgatcagga agtcgtggtt
     8761 gataaagatc agctggctac cagccggaca ccggacgatc tgccagcgtt taaccgcgag
     8821 gcgttacgcc tgctcggtgc ctgagtcgcg tagccaaatc atttttttac caaagcccag
     8881 cgtgttgtcg gtgaatttga tttcatcgag ccggatttcc cacaccgggg ccgacagcat
     8941 tctggcaacc ggaaagcgac gattgtacgc cttgcgcgcg aggtcgcttt cctcaccttc
     9001 cagcctgcgg atctcacctt taaactgcac accgcgaatt aacgctaccg ttttcggctg
     9061 accgtttacc gttccggcaa ccgcagcctg cggcccactc atctgcgcgt ggcgcgtttt
     9121 ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca aaaagataaa aggcattagc
     9181 gcaccagagt tccccttcct gctgcacaca ccaggtgaca acatgttgtt ttgccagcca
     9241 acggctgatg gcgatgagtg tttccattgc tgttctcttt tatactgtgg gcctgacctt
     9301 aacatgcgat gcagacgatg acaccctggt ttctttactt gatccgtacc gccgacaata
     9361 agctttatac cgggatcacc acggatgtcg aacgccgcta tcagcagcac caaagcggca
     9421 aaggggcgaa agcactgcgc gggaaaggag aactgacgct ggcgttttct gcgccagtgg
     9481 gcgaccgttc gctggcacta cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa
     9541 aagagcgtct ggtagcggaa ggcgcagggt ttgcagaact gttgagcagc ctgcaaaccc
     9601 cggagattaa aagcgattga aatgctcgtg atactcaacc agaccggtta cgccattcag
     9661 cgcgtcatcc gctaaacgat gtacctggaa ggcgctttca gtgcccggcc agcggcaacg
     9721 cagatcgtga tgcgccgcca gttcaaagcc gaaacgactg tacagcgccg gatcgcccag
     9781 cgtcaccact gcggcatagc cgaactcatt aagcgaatcg agtccttcat agaccagttg
     9841 gcgtgccagc ccctgtccac ggtatttttc atcaaccgcc aacggtgcca tgccgaccca
     9901 ttgcaggtct tcgccctgca catcaaccgg actaaatgcc acatagccaa tgacctgacc
     9961 ttcgtcatct gtcgccacca gccccagcgt cagaaagcca tcttcacgca gatcgtgaac
    10021 cagcttcgct tccgcatcac tttcgaatga gcgacgcagc agggcatcaa tacccggcgc
    10081 atcaatggga atttctactc gaattagcat ggttcaccta ccgatgtctg tttggtttca
    10141 ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag
    10201 gctttcggca tttgctccag ttcaatggcg tccatcaggt ttttcacata catccccagc
    10261 tccgtatcgc cttcaatcac cagccgacgc tggaagaaga gcgtatccgg atcttgttta
    10321 cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa aactcacatc agcttgcgcg
    10381 ttctggctga ccaccagttt gccattcacc accgaggtaa accattgcag gtcaatatca
    10441 cgcacatgaa tacttaacca gcggccttca agaaactcca gctcgccatc atccagcgcc
    10501 tgacggaatt gccagcttaa gacctgctca agaacctggc gttttagcgc aaatggcgtc
    10561 agttttaccg gtacactcaa cagagatggc cccaaatgca caatacggga acgcagttta
    10621 tccaacacga gttttactcc ctgtttcaac aatcatccta ttttgccata tcagaaaaat
    10681 aacatagcgg tataaatcaa caattccata tgaaattgct gctaccacca atacaacttt
    10741 aactgcctta aatcaaaaat tgtcgcagca aggttaacta aaatcccagt tcgttaacat
    10801 ttttacgttt tgatagcgca accttcagga aaaattatgg agctgctctg ccctgccgga
    10861 aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag atgctgttta tatcgggcta
    10921 aaagatgata ccaatgcccg tcacttcgcc ggccttaact ttaccgagaa aaaattgcag
    10981 gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc acatcgcgat taacactttt
    11041 gcgcatccgg acgattacgc ccgttggcag cgtgccgtgg atatggcggc gcagctgggt
    11101 gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt acgccgccga gcgttacccg
    11161 catattgaac gtcatgtgtc agtgcaggct tcggcgacca atgaagaggc gattaacttt
    11221 tatcatcgcc attttgacgt tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag
    11281 gtgaaacagc tggcacgggt tactcctgtc ccgctggaag tgtttgcttt cggcagcctg
    11341 tgcattatgt cggaaggccg ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac
    11401 actgtgggtg cgtgttctcc ggcccgtttc gtgcgctggc aacaaacgcc gcaggggctg
    11461 gaatcccgcc tgaacgaagt gctgatcgac cgttatcagg acggcgaaaa cgcaggttat
    11521 ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc gctatcacgc gctggaagaa
    11581 ccaaccagtc tcaataccct ggaactgctg ccggagttaa tggctgcgaa tattgcttcg
    11641 gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg
    11701 cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg
    11761 atggagacgc tcgggtcgat gtccgaaggc acgcaaacca cccttggcgc gtatcaccgt
    11821 aaatggcagt gagaagagca atgaaatatt ccttagggcc agtgctgtgg tactggccaa
    11881 aagagacgct ggaagatttt tatcagcagg ccgccgccag cagcgccgac gtgatttatc
    11941 ttggtgaagc ggtatgcagc aagcgccggg caaccaaagt tggcgactgt ctggagatgg
    12001 caaaatcgct cgccggaagt ggcaagcaga ttgtgctctc cacgctggcg ctggtgcagg
    12061 catcctctga actgggcgaa ctgaaacgct atgttgagaa cggtgagttt ctgattgaag
    12121 ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc
    12181 acgcgctgaa ctgctacaac gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc
    12241 gctggtgtat gccggtggag ctttcccgcg actggctggt gaatctgctt aatcagtgcg
    12301 atgagctggg cattcgcaac cagtttgaag tggaagtcct gagctacggt catctaccgc
    12361 tggcctactc cgcccgctgc tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg
    12421 aaacctgctg cattaagtat ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag
    12481 tgtttgtact caatggcatt cagaccatga gcggctacgt ttacaacctc ggtaacgagc
    12541 tggcatccat gcagggattg gttgatgtag tccgcctgtc accgcagggc actgacactt
    12601 tcgcgatgct cgacgccttc cgcgctaatg aaaatggcgc tgtgccactg ccgttgacgg
    12661 caaacagcga ctgtaacggc tactggcggc ggctggcagg actggagctg caagcctaag
    12721 taaatagctc actttgttaa caactttaac tattctttaa tgcagtatta aagattaatc
    12781 ggtaacaaag tgagctgtta tgactgataa aaccattgcg ttttcgctac tcgatctggc
    12841 ccccataccc gaaggctctt cagcgcggga agcgttctcc cactctctcg atctcgcccg
    12901 tctggctgaa aagcgcggct atcatcgcta ctggctggca gaacaccaca atatgaccgg
    12961 cattgccagt gctgcaacat cggtattgat tggctatctg gcggcgaata ccaccacgct
    13021 gcatctgggg tctggcggcg tgatgttgcc taaccactca ccgttggtca ttgccgaaca
    13081 gttcggcaca ctcaatacgc tctatccggg gcgaatcgat ttggggctgg gtcgtgctcc
    13141 gggtagtgac caacggacaa tgatggcgct acgtcgtcat atgagcggcg atattgataa
    13201 tttcccccgc gatgtcgcgg agctggtgga ctggtttgac gcccgcgatc ccaatccgca
    13261 tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg tggttgttag gctccagcct
    13321 ttacagtgca caactggcgg cgcagcttgg tctgccattt gcgtttgcct cacacttcgc
    13381 gccggatatg ttgttccagg cgctgcatct ttatcgcagt aacttcaaac cgtcggctcg
    13441 gctggaaaaa ccatacgcga tggtgtgcat caatattatc gccgccgaca gcaaccgcga
    13501 cgctgaattt ctgtttacct caatgcagca agcctttgtg aagctgcgcc gtggcgaaac
    13561 cgggcaactg ccgccgccga ttcaaaatat ggatcagttc tggtcaccgt ctgagcagta
    13621 tggcgtgcag caggcgctga gtatgtcgtt ggtaggcgat aaagcgaaag tgcgtcatgg
    13681 tttgcagtcg atcctgcgcg aaaccgacgc tgatgagatt atggttaacg ggcagatttt
    13741 cgaccaccag gcgcgcctgc attcgtttga gctggcgatg gatgttaagg aagagttgtt
    13801 gggatagtgt gtcttaacgc gggaagcctt atccgagctg gcaacgctgt cctacgtagg
    13861 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
    13921 ctgattactg atacaccggc agtaaattaa agctcgataa aatatgcacc agtgcgttgc
    13981 cgacgccaaa caccagaatc agcgcaatca tcggcttgcc accccagacg cggaatttcg
    14041 ggctgccaaa gcgtttacgc gatgcgcggg ccagcagtgc cggaacaatt gccgcccaga
    14101 tggtagccgc taaaccagca taaccaatgg catacaggaa tccgttcggg aacagcagac
    14161 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcggccc acagccgagt
    14221 cgtcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
    14281 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
    14341 gcacgccgct taatgcttgt accagcacat caatattacc gcccttctcg gcgatgccga
    14401 taaactccgg acgagggatg ttgcccattg tcgccagcaa ccagatggta tacagcgcca
    14461 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
    14521 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaggg
    14581 tcatcagcaa atacggcgca taagatgcat tgctttcggc aacgttgaac aatgtcgcag
    14641 gctgcacatg ccccagcagg ctaccgaagg tgaggaagaa ggtaatgact ttcgccccca
    14701 gcacaatcgc cgtcatgcgg ctgacggctt tagtactcaa ccacaccaca aacgctacca
    14761 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
    14821 tgtgatgcag aatcgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
    14881 aggcaatgga aatgccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
    14941 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
    15001 gcagcataca gaaccaggta aagatcagcg ctgccattga ccagaaaaac cacgccccgg
    15061 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
    15121 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
    15181 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acatgagtac
    15241 aaaagacaga aaaaaagccc cgatggttaa aatcggggct gtatatatta ttttacagat
    15301 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
    15361 catcacgacg cggtgcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
    15421 gaccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgc tcgccaccga
    15481 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgtgcatcg cccagtaact
    15541 gcatgttcat cggcttgttg agaatgcgag tgcgggtaaa gtgttgcagc acttcacccg
    15601 gcataccttt cggcagttcg atggtggagt gagaagcaaa cagcttgatg ttaccgatgt
    15661 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
    15721 catcatcgcg gcccacttca atgcggtaca gctgcatatc gccaacatca cgacgttcac
    15781 gacgcggacg atcttcacgg tcgccacgcg ggccacggtc gttacgatcg cgcggaccac
    15841 ggtcatcacg gtcacggaat tcacgtttcg gacgcatagg cgcatctggc ggaacaatca
    15901 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatccagct
    15961 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
    16021 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
    16081 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
    16141 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
    16201 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
    16261 ccaccaggct gatacgctca acgtccaggc cacgggccgc aacgtcggtc gcaatcagga
    16321 tgtccagacg accgtcttta aggcgttcca gtgtctgctc acgcagcgcc tggttcatgt
    16381 caccgttcag cgcggcgcta ttgtagccgt tacgctcaag agcttcagcc acttccagag
    16441 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
    16501 gtaccagtgc ttcgttttta cgcatacccc acacagtcca gtagctctgg ctgatgtcag
    16561 gacgggtagt cacgctggac tgaatacgca cttcctgcgg ctctttcata aagcggcggg
    16621 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
    16681 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
    16741 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
    16801 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
    16861 gcacgtcata acgctggccg ccgtacaggg caaccacatt tacgccgcgc atgtgtttag
    16921 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
    16981 gaatctgtgg tgctttcagc tcaggatcaa gattctgcag cagaggtaaa gagaatgctg
    17041 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
    17101 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
    17161 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
    17221 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
    17281 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
    17341 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
    17401 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
    17461 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
    17521 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
    17581 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
    17641 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
    17701 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
    17761 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
    17821 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
    17881 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
    17941 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
    18001 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
    18061 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
    18121 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
    18181 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
    18241 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
    18301 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
    18361 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
    18421 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
    18481 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
    18541 ctcaacgcgt ttgtcagcga tttgagagat gtgtaccaga ccttctttac cgccgccgat
    18601 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcagcccac
    18661 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
    18721 ggtcgctgca atcttcacag taccgtcatc ttcgatttcg atggtggtgc cagtttcttc
    18781 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
    18841 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
    18901 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
    18961 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
    19021 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
    19081 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
    19141 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
    19201 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
    19261 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
    19321 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
    19381 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
    19441 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
    19501 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
    19561 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacag cgtttttctc
    19621 gatcgcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
    19681 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
    19741 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
    19801 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
    19861 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
    19921 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agtacagctg cttcagtacc
    19981 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
    20041 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
    20101 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
    20161 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
    20221 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
    20281 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
    20341 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
    20401 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
    20461 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
    20521 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
    20581 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
    20641 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
    20701 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
    20761 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
    20821 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
    20881 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
    20941 cagcagtgct acctgaactt cagtagaacc ggtgtcgttt gcgtcacgac caaactcaga
    21001 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
    21061 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
    21121 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
    21181 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
    21241 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
    21301 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
    21361 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
    21421 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cacagatgtt
    21481 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
    21541 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
    21601 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
    21661 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacac gcatattcgt
    21721 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
    21781 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
    21841 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
    21901 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
    21961 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
    22021 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc ggtttatcca
    22081 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
    22141 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
    22201 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
    22261 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
    22321 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
    22381 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
    22441 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
    22501 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
    22561 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
    22621 cgccagcgtc acatcaggca atccatgccg gatgcggcgt aaacgcctta tcccgcatgg
    22681 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
    22741 catcgccagt gcggacgtcg ttgtagttct taacgccaat accacattcc ataccgttac
    22801 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
    22861 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
    22921 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
    22981 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
    23041 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
    23101 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
    23161 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
    23221 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
    23281 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
    23341 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
    23401 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
    23461 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
    23521 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
    23581 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
    23641 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
    23701 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatacca gtacccgctt
    23761 tcgcagacac atgtacgaac tggctttcac cgccccactc ttccggcagg atgccgtact
    23821 gggagagttc gttcttaacg cgatccggat cggcttctgg tttatcgatc ttgttcactg
    23881 caacgaccac cggaacctgc gccgctttcg cgtgctggat tgcttcgatc gtctgcggca
    23941 ttacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgag
    24001 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    24061 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcca
    24121 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    24181 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    24241 cctcttccag ctcgttttca cgacgcagga taactttatg gcccatctct tcagcaacca
    24301 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    24361 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    24421 tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct
    24481 gcagcgaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    24541 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    24601 tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    24661 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    24721 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    24781 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    24841 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    24901 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    24961 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    25021 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    25081 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    25141 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    25201 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    25261 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    25321 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    25381 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    25441 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    25501 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    25561 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    25621 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    25681 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    25741 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    25801 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    25861 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    25921 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    25981 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    26041 ggttatcatc ccacagaacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    26101 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    26161 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    26221 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    26281 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    26341 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    26401 cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    26461 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    26521 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    26581 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    26641 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    26701 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    26761 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    26821 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    26881 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    26941 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    27001 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    27061 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    27121 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    27181 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    27241 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    27301 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    27361 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    27421 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    27481 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    27541 ttgaaaaagg actctaaggg aaa
//
LOCUS       DADKXM010000044        25945 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.044, whole genome shotgun sequence.
ACCESSION   DADKXM010000044 DADKXM010000000
VERSION     DADKXM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.044"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            223..1122
                     /gene="lysO"
                     /locus_tag="JQR05_001823"
     CDS             223..1122
                     /gene="lysO"
                     /locus_tag="JQR05_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY5324008.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1266..2918
                     /gene="hcp"
                     /locus_tag="JQR05_001824"
                     /gene_synonym="priS"
     CDS             1266..2918
                     /gene="hcp"
                     /locus_tag="JQR05_001824"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY5324009.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2930..3898
                     /gene="hcr"
                     /locus_tag="JQR05_001825"
     CDS             2930..3898
                     /gene="hcr"
                     /locus_tag="JQR05_001825"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY5324010.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4031..5749
                     /gene="poxB"
                     /locus_tag="JQR05_001826"
     CDS             4031..5749
                     /gene="poxB"
                     /locus_tag="JQR05_001826"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY5324011.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTKFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5786..6787
                     /gene="ltaE"
                     /locus_tag="JQR05_001827"
     CDS             5786..6787
                     /gene="ltaE"
                     /locus_tag="JQR05_001827"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY5324012.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSRILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEIAAHWRAFLAR"
     gene            6798..8228
                     /locus_tag="JQR05_001828"
     CDS             6798..8228
                     /locus_tag="JQR05_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5324013.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8327..9340
                     /locus_tag="JQR05_001829"
     CDS             8327..9340
                     /locus_tag="JQR05_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY5324014.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKDISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9337..10167)
                     /locus_tag="JQR05_001830"
     CDS             complement(9337..10167)
                     /locus_tag="JQR05_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY5324015.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTSVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10164..10487)
                     /locus_tag="JQR05_001831"
     CDS             complement(10164..10487)
                     /locus_tag="JQR05_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY5324016.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10698..12467)
                     /locus_tag="JQR05_001832"
     CDS             complement(10698..12467)
                     /locus_tag="JQR05_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY5324017.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            13116..13631
                     /locus_tag="JQR05_001833"
     CDS             13116..13631
                     /locus_tag="JQR05_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5324018.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            13849..14577
                     /gene="artP"
                     /locus_tag="JQR05_001834"
     CDS             13849..14577
                     /gene="artP"
                     /locus_tag="JQR05_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY5324019.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCSVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14595..15326
                     /gene="artJ"
                     /locus_tag="JQR05_001835"
     CDS             14595..15326
                     /gene="artJ"
                     /locus_tag="JQR05_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5324020.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15333..16049
                     /gene="artQ"
                     /locus_tag="JQR05_001836"
     CDS             15333..16049
                     /gene="artQ"
                     /locus_tag="JQR05_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY5324021.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16049..16717
                     /gene="artM"
                     /locus_tag="JQR05_001837"
     CDS             16049..16717
                     /gene="artM"
                     /locus_tag="JQR05_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY5324022.1"
                     /translation="MFEYLPELMKGLHTSLTLTFASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17009..17740
                     /gene="artJ"
                     /locus_tag="JQR05_001838"
     CDS             17009..17740
                     /gene="artJ"
                     /locus_tag="JQR05_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5324023.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17915..19042)
                     /gene="rlmC"
                     /locus_tag="JQR05_001839"
     CDS             complement(17915..19042)
                     /gene="rlmC"
                     /locus_tag="JQR05_001839"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY5324024.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NTQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(19083..19571)
                     /locus_tag="JQR05_001840"
     CDS             complement(19083..19571)
                     /locus_tag="JQR05_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY5324025.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(19631..20437)
                     /gene="potI"
                     /locus_tag="JQR05_001841"
     CDS             complement(19631..20437)
                     /gene="potI"
                     /locus_tag="JQR05_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY5324026.1"
                     /translation="MILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLR
                     DDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDV
                     ITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEA
                     AMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVF
                     SSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG"
     gene            complement(20473..21426)
                     /gene="potH"
                     /locus_tag="JQR05_001842"
     CDS             complement(20473..21426)
                     /gene="potH"
                     /locus_tag="JQR05_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY5324027.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21436..22569)
                     /gene="potG"
                     /locus_tag="JQR05_001843"
     CDS             complement(21436..22569)
                     /gene="potG"
                     /locus_tag="JQR05_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY5324028.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(22664..23776)
                     /gene="potF"
                     /locus_tag="JQR05_001844"
     CDS             complement(22664..23776)
                     /gene="potF"
                     /locus_tag="JQR05_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY5324029.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24127..24603)
                     /locus_tag="JQR05_001845"
     CDS             complement(24127..24603)
                     /locus_tag="JQR05_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY5324030.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(24691..25593)
                     /gene="rimK"
                     /locus_tag="JQR05_001846"
     CDS             complement(24691..25593)
                     /gene="rimK"
                     /locus_tag="JQR05_001846"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY5324031.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERWAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(25654..>25945)
                     /gene="nfsA"
                     /locus_tag="JQR05_001847"
     CDS             complement(25654..>25945)
                     /gene="nfsA"
                     /locus_tag="JQR05_001847"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY5324032.1"
                     /translation="LPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKDA
                     LAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR"
BASE COUNT         6440 a         6742 c         6401 g         6362 t
ORIGIN      
        1 gggggcggcg gcgactggct gccccctgcg cgctccctcc gccagtggct ttatatggca
       61 ttgcacatga aaggagcgca acaatgctaa acactcagat aggattaaaa atccccctct
      121 gatgcataac atttcctttt aattgcaact ttactcgtcc ttccgctttc agtagtgtgt
      181 catcgcgcat ttcgacttct tctttgcaag ggctggattt tcatgttttc tgggctgtta
      241 atcattctgg ttcccctgat tgtgggttac ctcattccgc ttcgccaaca agctgcgtta
      301 aaagttatta atcagctatt aagctggatg gtttacctta ttctcttttt tatgggtatc
      361 agtctggcgt ttctcgataa cctcgccagt aacctgttgg cgattctgca ttattctgcc
      421 gtcagtatta ccgttatttt actgtgtaat attgccgccc tgatgtggct ggagcgaggc
      481 ctgccgtggc gcaaccacca tcagcaagaa aaactcccgt cgcgtattgc gatggcgctg
      541 gagtcgctaa aactgtgcgg cgtagtagtg attggttttg ccattggtct gagtggactg
      601 gctttcttac aacacgcgac cgaagccagt gaatacacgt taattttgct acttttcctc
      661 gttggtattc agttgcgcaa taatggcatg accttaaagc agattgtcct taatcgccgg
      721 ggaatgattg tcgccgtggt ggtggttgtc agttcattaa ttggtggttt aattaacgcc
      781 tttattcttg atctccccat caataccgcg ctggcaatgg cctccggttt cggctggtat
      841 tctctttccg gtattttatt gaccgaatct tttggtccgg taatcgggag cgcggcgttt
      901 tttaatgatc tggcccgtga actgattgct attatgttga tccctgggct gattcgccgc
      961 agccgctcta ctgcactggg cttatgcggt gccacatcaa tggatttcac cctgcccgtt
     1021 cttcaacgta ctggcgggct ggatatggtc ccggcggcaa ttgttcacgg ttttattctt
     1081 agcctgttag tgccgatcct catcgccttt ttctctgcgt aatacctctc tggcggtaga
     1141 tccctgccgc caaaattgcg ctaaatcaat ctcccttaaa gttgcatgaa aaatcccttt
     1201 tatccccgcg ttaagcgtct taaccttaaa catgtatatt aaatataact ttaaaaggtg
     1261 tgatcatgtt ttgtgtgcaa tgtgaacaaa ctatccgtac tccggcagga aacggctgct
     1321 catacgcgca ggggatgtgt ggtaaaacgg cggaaacttc tgaccttcag gatttactca
     1381 tcgcggcgct gcaagggctt tctgcctggg cggtaaaagc gcgtgaatac ggcatcatca
     1441 accacgatgt tgacagcttc gcgccacgtg cctttttctc aaccctgacc aacgttaact
     1501 tcgattctcc gcgtattgtc ggctacgctc gtgaagcgat tgccttgcgc gaggcgctga
     1561 aagcacaatg cctggctgta gatgccaacg cgcgcgtcga taacccgatg gctgacctgc
     1621 aactggtgag cgatgatctc ggcgagctgc aacgtcaggc agcagaattt acccctaaca
     1681 aagataaagc ggcgattggc gaaaacattc tcggcctgcg tctgctgtgc ctgtatggcc
     1741 tgaaaggtgc ggcggcctat atggaacacg cgcatgttct cggtcaatac gacaacgata
     1801 tttatgccca gtaccataaa atcatggcgt ggctggggac ctggcctgcc gatatgaacg
     1861 cgcttcttga gtgttcaatg gaaatcggcc agatgaactt caaagtgatg agcattctgg
     1921 atgcaggcga aaccggtaaa tacggtcacc cgacgccaac ccaggtcaac gtcaaagcga
     1981 cggcgggtaa atgcattctg atttccggtc acgatctcaa agatctctac aacctgctgg
     2041 aacagaccga aggcacgggc gttaatgtct acacccacgg cgaaatgctg cctgcgcatg
     2101 gctacccgga gctgcgtaaa ttcaagcatc tggtcggtaa ctacggcagc ggctggcaga
     2161 atcagcaagt ggagttcgct cgtttccctg gccccatcgt gatgacctcg aactgcatca
     2221 tcgacccaac cgtaggcgct tatgacgatc gtatctggac ccgcagcatc gttggctggc
     2281 ctggcgtgcg tcatctggat ggcgaagatt tctctgcggt catcgcccag gcgcaacaaa
     2341 tggcgggctt cccgtacagc gaaattcctc acctgatcac cgtgggcttt ggtcgccaga
     2401 cgctgcttgg tgcagccgat acgctgattg atctggttag ccgtgaaaaa ctgcgtcata
     2461 tcttcctgct tggtggctgt gacggcgcac gcggcgagcg ccactacttc accgatttcg
     2521 ccaccagcgt gccggatgac tgcctgatcc tgaccctcgc ctgtggtaaa tatcgcttta
     2581 acaaacttga gtttggcgat atcgaaggtc tgccgcgtct ggtagatgca ggtcaatgta
     2641 acgatgctta ctcagcaatt attctggctg tcactctggc agagaaactg ggctgtggtg
     2701 tgaacgatct gccgctgtcg ctggtgctct cctggtttga acagaaagcg attgtcattc
     2761 tgctgacgct gctttctctg ggcgtgaaaa atatcgtcac cggcccgact gcacctggtt
     2821 tcctgacacc agacctgctg gcggtgctga acgagaaatt cggcctgcgt tctatcacca
     2881 ctgttgaaga agacatgaag caactgttga gcgcgtaagg agtttgtcga tgacgatgcc
     2941 aacgaatcaa tgcccgtggc ggatgcaggt tcatcacatt acgcaagaaa cgccggatgt
     3001 gtggacgatt tccctgattt gccacgatca ctacccatat cgcgccgggc aatatgcact
     3061 ggtcagcgtg cgaaactcag cggaaacgct gcgtgcttac accatttcct ccacgccagg
     3121 cgtgagtgaa tatatcaccc tgaccgtgcg gcggattgat gacggtgtcg gctcccagtg
     3181 gctgacgcgc gatgtaaaac gcggtgatta tctctggctt tcggacgcga tgggggaatt
     3241 tacctgcgac gataaagcag aagataaatt cctgttgctg gcggcaggct gcggcgtcac
     3301 gccgattatg tcgatgcgtc gctggcttgc gaagaaccgt ccacaggccg atgtgcgggt
     3361 gatctacaac gtgcgtacgc cgcaggatgt tattttcgcc gatgagtggc gtaactatcc
     3421 ggtaacgctg gtggcagaaa ataacgttac cgaaggcttt atcgctggtc gtctcactcg
     3481 cgaactgctg gcaggtgtac ctgacttagc ttcacgtacc gtgatgacct gcggcccggc
     3541 tccgtatatg gattgggtag agcaggaagt gaaagcgctc ggcgtgacgc gtttctttaa
     3601 agagaaattc ttcaccccag tagcagaagc ggcgaccagc ggtctgaaat tcaccaaact
     3661 gcaaccggca cgagaatttt acgccccggt tggcaccacg ctactggagg cgctggaaag
     3721 caataacgtt ccggttgtcg ctgcctgccg tgcgggtgtt tgcggctgct gtaagacaaa
     3781 agtggtttcc ggtgaatata cggtgagcag tacaatgacg ctgaccgacg ccgaaatcgc
     3841 tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc tcgcataatc
     3901 gccttatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc ctcctttctc
     3961 tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca cattcaggag
     4021 atggagaacc atgaaacaaa cggttgcagc ttatatcgcc aaaacactcg aatcggcagg
     4081 cgtgaaacgc atctggggag tcacgggcga ctctctgaac ggtcttagcg acagtcttaa
     4141 tcgcatgggc accatcgagt ggatgtccac ccgccatgaa gaagtggcgg cgtttgccgc
     4201 tggcgctgaa gcacaacttt caggagaact ggcggtctgt gccggatcgt gcggcccagg
     4261 caacctgcac ttgatcaacg gcctattcga ttgccaccgc aataacgttc cggtactggc
     4321 gattgccgct catattccct ccagcgaaat tggcagcggc tatttccagg aaacccaccc
     4381 acaagagcta ttccgcgaat gtagtcacta ttgcgagctg gtttccagcc cggagcagat
     4441 cccacaagtg ctggcaattg ctatgcgcaa agcggtgctt aaccgtggcg tttccgttgt
     4501 tgtgttaccg ggcgacgtgg cgttaaaacc tgcgccagaa ggggcaacca cgcactggta
     4561 tcatgcgcca cagccagtcg tgacgccgga agaagaagag ttacgcaaac tggcgcaact
     4621 gctgcgttat tccagcaata tcgccctgat gtgtggcagc ggctgcgcgg gggcgcataa
     4681 agagttagtt gagtttgccg ggaaaattaa agcacctatt gttcatgccc tacgcggtaa
     4741 agagcatgtc gaatacgata atccgtatga tgttggaatg accgggttaa tcggattctc
     4801 gtcaggtttc cataccatga tgaacgccga cacgttagtg ctactcggta ctcagttccc
     4861 ctaccgtgcg ttctacccga ccgatgccaa aattattcag attgatatca atccagccag
     4921 catcggcgcg catagcaagg tagatatggc gctggtcggc gatatcaaat caaccctgcg
     4981 cgcattgctg ccactggtgg aagaaaaaac cgatcgcaag tttctggata aagcgctgga
     5041 agattaccgc gatgcccgca aagggctgga cgatttagct aaaccgagcg agaaagccat
     5101 tcacccgcaa tatctggcgc agcaaattag tcattttgcc gccgatgacg ccatctttac
     5161 ctgtgacgtc ggcacgccaa cggtatgggc ggcacgttat ctgaaaatga acggcaagcg
     5221 tcgcctgtta ggttcgttta accacggttc gatggctaac gccatgccgc aggcgttggg
     5281 tgcgcaggca accgagccgg aacgtcaggt ggtcgccatg tgcggcgatg gcggttttag
     5341 catgttgatg ggcgatttcc tctcagtagt gcagatgaaa ctgccggtga aaattatcgt
     5401 ctttaataac agcgtgctgg gctttgtggc gatggagatg aaggccggag gttacctgac
     5461 agacggtact gagctgcacg acaccaaatt tgcccgcatt gccgaagcgt gcggcattac
     5521 gggtatccgt gtagaaaaag cctctgaagt tgatgaagcc ctgcaacgcg ccttctctat
     5581 cgacggtccg gtgttggtgg atgtggtggt cgccaaagaa gaattagcca ttccaccgca
     5641 gatcaaactc gaacaggcca aaggttttag cctgtatatg ttgcgcgcaa ttatcagcgg
     5701 acgcggcgat gaagtgatcg aactggcgaa aacgaactgg ctaaggtaaa aagggtggca
     5761 tttcccgtca taataaggac atgccatgat tgatttacgc agtgataccg ttacccgacc
     5821 gagccgcgcc atgctcgaag cgatgatggc cgccccggtt ggggacgacg tttacggaga
     5881 cgaccctacc gttaatgctc tgcaggacta tgcagcagag ctttccggta aagaagccgc
     5941 catttttctg cctaccggca ctcaggccaa cctggtcgct ctgctcagtc actgcgaacg
     6001 tggcgaagag tatattgtcg gtcaggccgc gcataactat ctgtttgaag ccggtggcgc
     6061 agcggtgctg ggcagtattc agccgcaacc catcgacgcg gctgccgacg gcacgctacc
     6121 gctggataaa gtggcgatga aaatcaaacc cgacgatatc catttcgccc gcaccaaatt
     6181 actcagtctg gaaaacaccc acaacggcaa agtgctgccg cgtgaatacc tgaaagaagc
     6241 atgggaattt acccgcgagc gcaatctggc gttgcatgtt gacggtgcgc gcatctttaa
     6301 tgccgtggtg gcttacggct gcgaactgaa agagatcacg caatattgtg attcgttcac
     6361 catttgcctg tcgaaaggtc ttgggacgcc agtcggttca ttactcgtcg gtaatcgtga
     6421 ttacattaaa cgtgccatcc gctggcggaa aatggccggt ggcgggatgc gtcagtccag
     6481 gattctggct gccgccggga tgtatgccct gaaaaataac gtcgcacggt tgcaggaaga
     6541 tcacgacaac gccgcctgga tggcggagca actgcgtgaa gcaggcgcgg atgtgatgcg
     6601 tcaggacact aatatgctgt ttgttcgcgt cggggaagaa aatgctgccg cgttaggcga
     6661 atacatgaaa gcgagaaacg tgctgattaa cgcctcgccg attgtccgcc tggtgacgca
     6721 tcttgacgtc tcgcgcgaac aactggcgga aatcgccgcc cactggcgcg cattcctggc
     6781 gcgttaagga gagagacgtg ccgcaacgca ttttagttct cggtgccagt ggctacattg
     6841 gtcagcatct ggtgcgcaca ctcagccagc aagggcatca gatcctggcg gcggcacgtc
     6901 atgtcgacag gcttgcaaag ctgcaactgg caaacgtcag ttgccataaa gtcgatctca
     6961 gctggccgga taaccttccg gccctgttgc aggatatcga taccgtctat tttctggtgc
     7021 acagcatggg cgaaggcggc gattttatcg ctcaggagcg ccaggtggct ctcaacgtcc
     7081 gcgatgcgct acgtgaagta ccagttaagc aattaatctt tctcagttcg ttgcaggccc
     7141 cgccacatga gcagtcggat catctgcgtg ctcgtcaggc tacggcggac attcttcgtg
     7201 aagcgaatgt accagtgacc gaacttcggg ccggaattat cgttggcgca ggttcagcgg
     7261 cgttcgaagt catgcgcgat atggtctaca acctgccagt gttaacgccg ccacgctggg
     7321 tacgttcacg caccacgccc atcgcgctgg aaaacttgct gcactatctg gtggcgctgt
     7381 tagaccatcc agccaacgaa caccgcatct tcgaagccgc cggaccagag gtgctcagtt
     7441 atcagcaaca gtttgaacat tttatggcgg tgagcggtaa gcgccgctgg ttgatcccca
     7501 tccccctccc cacccgctgg atttcggtgt ggtttctcaa tgtgattact tccgtaccgc
     7561 ccaccaccgc cagggcgttg attcaggggc tgaaacacga tctgctggca gatgataccg
     7621 cgctacgtgc actcatccca caacggctga tcgctttcga tgacgcggta cgtagcacat
     7681 tgaaagagga agaaaaactg gtcaactcca gcgactgggg ctacgacgct caggcctttg
     7741 cccgctggcg accggagtac ggttatttcg ccaaacaggc ggggtttacc gttaaaacgt
     7801 ccgccagcct tgcggcttta tggctggtgg tgaaccaaat cggcggtaaa gagcgttatt
     7861 tctttggcaa tattttgtgg cagacacggg cgttgatgga ccgcgcgatc ggtcataatc
     7921 tggcgaaagg ccgcccggag cgcgaatatt tacagaccgg cgatgcggtg gatagctgga
     7981 aagtgattgt cgttgaaccg aaaaagcaac ttacgttgtt atttggcatg aaagcgccgg
     8041 ggctgggacg actgtgtttt accctggaag ataaaggtga ctatcgtact atcgatgtcc
     8101 gcgctttctg gcatccgcac ggtatgccgg ggctgtttta ctggttattg atgatccccg
     8161 cgcatctgtt tatttttcgc ggaatggcaa aacaaatcgc cagactggca gaacaaagca
     8221 cagattaatc gacaaaacct gcgttattct ttcatgtttc ccattgcatc atgggggaaa
     8281 tcacggaaga atgcgcacgt cattttctca acaacagtgg attgatatga aggtactggt
     8341 taccggcgcc accagcggct taggtcgaaa cgcggtagag tttttatgcc agaaagacat
     8401 cagcgtgcgt gcgagcggtc gcaacgaagc aatgggtaaa ttgctggaaa aaatgggcgc
     8461 agagtttgtt ccgacggatc tgaccgagct ggtttcatca caagctaaag tgatgctcgc
     8521 gggcattgat acgctgtggc actgctccag ctttacctca ccctggggga cacaacaggc
     8581 tttcgatctg gctaacgttc gcgccactcg ccgcctgggt gaatgggctg tcgcctgggg
     8641 tgtgcgtaac tttattcata tctcttcccc ctccctgtac ttcgattatc accaccatcg
     8701 cgatattaaa gaagattttc gccctcaccg cttcgccaac gagtttgccc gcagcaaagc
     8761 agccagcgaa gaagtgatca atatgctttc gcaggcgaat ccacaaacgc gctttactat
     8821 tctgcgccca caaagtctgt tcggaccgca cgataaagtc tttattcccc gtctggcgca
     8881 tatgatgcac cactacggca gtattctgtt accgcatggc ggcagtgcgc tggtcgatat
     8941 gacctactat gaaaatgccg tgcacgcaat gtggctggca agccaggaag cctgcgataa
     9001 gctaccttcc gggcgtgtgt acaacatcac caacggcgag catcgcacac tgcgcagcat
     9061 cgtgcagaag ctgatcgacg agttgaatat tgactgtcgt attcgttccg tcccttaccc
     9121 gatgctggat atgatcgccc gcagcatgga gcgtttaggc cgcaagtcag caaaagagcc
     9181 gccgctgacc cactacggcg tctccaagtt gaattttgac tttacgctgg atattacgcg
     9241 tgcgcaggaa gagttaggtt atcagccggt catcaccctg gatgaaggca tcgagaaaac
     9301 tgccgcctgg ctacgcgacc acggaaaact gccgcgctaa tcctgcccgt atttctccag
     9361 caatgcttcg gcaatcgcct gagtttctgc atccgcttcg ccgttatata acgtcgggcg
     9421 gaaatgcatc tggaatgcca taatcacgcg ccgctgctcg cgcggtgtca tatcaggttt
     9481 tacgtcataa ccgtagcgcg ccaaaagctc cagcaatgac gcggtatcta ccgatgtgtg
     9541 cggcgcgcgc ccggcaaggt aaaagttcac ccgctgcgca tccggccagg caccaatccc
     9601 ctgctgcgcc agttgctgcc agggaaataa tggccccgga tcgtctttgc gctgcggtgc
     9661 gatatccgca tgtgccacta cgttttccgg cttgatgtga taacgggcaa taatatcttt
     9721 cgccagcgga ataagcgcct gaatctgtgc ctgttcaaac ggggcaaaat atttcactcc
     9781 ggatgatttt tgccagccac ggttttccag ctcaatgcca atagaggtgt cgttaaggcg
     9841 cgttgccccg cgccaggcgc taatccccgc atgccaggcc agttcttgtt ccggcaccag
     9901 ttgccagatg cgcggtttac cgttgtatcg cggtggcacc gcagggatca gataatgcga
     9961 gctgacctgc ttatcagtca gtgtcgccag cgagctatca aaatcatctg cggtgtagtg
    10021 gatcaccagc actttaatgc gcggatacgc cgcctgcgcc tggtgtcggg tatcaagctg
    10081 atatccctct ttctcgacaa tgcctttttc gcctgcacac cctgccagta acagagcggc
    10141 agcgaccagc cagaagactc ttctcatcga cgcgttttca ccgccgtacc gctgacgcta
    10201 accatcagca tactgccgtt ttgcccgacc gtttcgtagt cgatatcaat accaactacg
    10261 gcatcggccc ccagcgcccg cgcctgggag cctaattcct caaaggcgat ctcccgtgct
    10321 ttacgcagtt ctttttcata cgcaccggaa cgtccgccaa cgatatcgcg gataccggca
    10381 aagaaatcac ggaaaatatt ggcacctaaa atcgcttcgc cggtcaccac accgcaatat
    10441 tcaacgatgg tctggccttc cagagttggg gttgtagaaa attgcatgat cgatctcctt
    10501 gtttgttaag ccttcgatct ctaaagcatt atcggactga tatgccatgt tgggaagaag
    10561 gttttgatca gtttaggaaa tcatctcatt gtgtcaaaaa cacccactta agtctttacg
    10621 attatccgtt gcataacccg atattggttt cattataggg taccgcttac ctgtcttatt
    10681 atgttgcacc aacaacatta atgagcggaa agaggcaact tatattgtaa atgactccta
    10741 aagtcttccc catcttgtga ctgaggtcta taccgctgga ttgctttttg gtcagccgcc
    10801 gcaaaacaag aaagtaataa gatctcaatg cttttgtcat taatataagc gaagatcatt
    10861 cttgcaaact cgtatataaa cgcccgatcc tgagcgctta atagtttaat acaatattta
    10921 tttttaagag agaaaataat ttcaaggaaa gcatccgcag caaccttcac gttattcccg
    10981 gcgttcatat tatatatatc ccttgcacag atagagtaca gagatagtaa acagccatac
    11041 aaataatcac ttattaattc ttttgtccat tttacaggca accagggttt tatcttgaca
    11101 ccatgcatac tacattcatg aaaaagacat ttgctgagat taactcttat catatcaaca
    11161 ttgaaaaaat tgcagttaat aaattttaaa gaatgagtat ttacgttatt aattttaaca
    11221 gatctaaacg aaatattttt gaatgcactg tttattacat tgctatttgt tattttaaca
    11281 tcacctaaac tgccgttttc aaaacaacat tttttcaata tgctttcatt cacaacagtg
    11341 tgtgataaat agagattact tatgttttgc tttattaatg ccacgtcatt aaaatgacta
    11401 tatttaatga aacaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
    11461 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
    11521 aagtgaatca tgcattgcgt tccctttgtc atatccacac ttacgctaca aatatcgtgg
    11581 taatgcgaag aatgcatttc aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat
    11641 cgtgttaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
    11701 agcactattg catgctctac atctaacgca gtttcaaatg actcttcttc tgtaaaatca
    11761 ttatttaaaa cctcacctga cgtattatca ctgtcataaa aagtgctcat caatgcgata
    11821 ttgtttgtta cagcgtcttt atcgatattc tcactaatag catatcgcct gctataatca
    11881 taaaaatgaa taaatgcgtc ctttataaag gatatatatt gttctttgtt aaagtcaata
    11941 cgactgtata acttttcaaa aatcacattc gcaatcttac taccaatggt atacctgtat
    12001 aataatgggt gcgattcaat cgtaaaatac ttatctaatt gttcttgtaa tttattctca
    12061 tagtccagtg ggaaaagaaa ctgttgtctt tcaagttttc cattcgatac attacgtatt
    12121 ccaatagaga ttctttcatt acccgctttt atttcaactt cgttatcttc tgatttaccc
    12181 agatattttt taacatcaat ctcgaaagca ttgctaaaaa atgtattaaa ttggtaattc
    12241 gctgatggaa tattttccgc gagcaacaca tcatttttta tgagatcact gtcagcattt
    12301 attgctaata tcagatgcaa gtagatggta tcccatgtag acaactcacc cttacgactg
    12361 gaatttaata tcgcgaaaaa ttccctcagt gaatccagga aattatcact ttgatttagt
    12421 tgtgtgcctg gaaatgatga tgcttgatca gatatattgt ttatcatatt aaaaatcacc
    12481 caagaaaaac gataatccac cgaactcctc gcgctttata attataaaag cacaacgcct
    12541 tttgtattca tattaattaa caaaatactg aagcaattta atttattctt cactcaaatt
    12601 cattagctta aattctttac ccacaattgt agcataaata atattatcga gagtcaaagc
    12661 tgggactatc aaatttcgct cattttcaaa aatacaacgt aattttggca atcatcttaa
    12721 tcgcaggatc tcttatcgca tgtgaaaaat tttgtaatga ttagacaata accgcattca
    12781 tttatcacgc gttataaatg tgaaactggc ttcttgcaga tgctcttaac ctctacagga
    12841 ggatttatac caccatttca gaactatcat atttacctta aatgatcatt tcagccatat
    12901 aacacgctca acctcacaaa tggtttacac tgtctattgt ttgggtaatt taagtaagtc
    12961 agctacgagc aaccttgagt gggtagccag caatggactc aaacccaatc aggtgttcag
    13021 attgtttcag aattgtatta aaaacaatgt attaaataat ctgatagcgt ttggtcgtag
    13081 tgtgacgcag acagcaagct aataaggaaa tgattatgcg ctaccgttca ctatctctac
    13141 ttattccatg tgcactgctg ctgagtgcct gtaccaccgt cacgccagct tataaagata
    13201 acggcccccg caccggttcc tgtgtccagg gagggcctga cagtgtggct caacaattct
    13261 acgactaccg cattcaacat cgcagcaatg acattaccgc cctgcgcccc tatttgagtg
    13321 acaaactggc aacccaactt agcgatgcca gccgcgataa tagtcatcgt gaattgttaa
    13381 gcagcgatcc gttctccagt cgaaccacct tgccagacag cgcccacgtt gccagtgcat
    13441 cgactatccc taatcgtgat gcccgtaaca ttccattgcg ggtggatctc aaacagggtg
    13501 accagggctg gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg
    13561 atgtgcgcta cttaggcggt agcgttcatg ccacggcggg tacgctccgt cagtccatcg
    13621 agaatcgcta acgccttgat caaccaataa accccgtaaa ttatccggca tttcgtgagg
    13681 aatgccgaca tttatgctca ccgaccaccg cccccgttat tttgtgctat gtttattgaa
    13741 taatgcgctt tgcttttaac ttttaaagca taaatattgc ataattattc tgtcaaaggt
    13801 actatcttcg acctcaatag ctatcagact gccagtatac gagtgtcaat gagtattcaa
    13861 ttaaacggca ttaattgctt ctacggcgcg catcaggcgc tgttcgatat cacgctggat
    13921 tgcccacagg gcgaaacgct ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg
    13981 ctgcgtgtac tcaatctgct tgagatgccg cgctccggta cgctcaacat tgcaggcaac
    14041 catttcgatt tcaccaaaac accctctgac aaagcgattc gcgatttgcg tcgtaacgtt
    14101 ggcatggtgt ttcagcaata caacctgtgg ccgcatctga ccgtgcagca aaacctgatt
    14161 gaagcgccct gcagtgtact ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa
    14221 ctgctggaac gtctgcgtct caaaccttat agcgatcgtt acccgctgca tctttctggt
    14281 ggtcagcagc agcgtgttgc tattgcccgt gcgttgatga tggaaccgca ggtactgctg
    14341 ttcgatgaac cgaccgccgc actggacccg gaaattacgg cacaaatcgt cagcatcatt
    14401 cgtgagctgg cagaaacgaa tattacccag gtgatcgtca cccacgaagt tgaagtggcg
    14461 cgtaaaaccg ccagccgagt ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac
    14521 gcgagctgct ttaccgagcc gcaaaccgaa gcatttaaaa actatctctc tcactaagat
    14581 tcgggataac aacaatgaaa aaagttctga ttgccgcgtt aattgcaggt tttagtcttt
    14641 ccgccacagc tgccgaaacc attcgttttg ctaccgaagc ctcctatcct ccgtttgaat
    14701 cgattgatgc aaacaaccag atcgttggtt ttgacgtcga cctggcacag gcactgtgta
    14761 aagagattga tgcaacctgt actttctcta accaggcgtt tgacagcctg atcccaagcc
    14821 tgaaattccg tcgcgtagaa gcagtgatgg cgggcatgga tatcactccg gagcgtgaaa
    14881 agcaggtact gtttaccacc ccgtactatg acaactctgc cctgtttgtg ggtcagcaag
    14941 gcaaatacac cagtgttgat cagctgaaag gcaaaaaagt cggcgttcag aacgggacga
    15001 cacaccagaa gttcattatg gataagcacc cggaaatcac caccgttccg tatgacagct
    15061 accagaacgc aaaactggat ctgcaaaacg ggcgtatcga cagcgtattt ggtgacaccg
    15121 cagtggtcac tgagtggctg aaagataacc caaaactggc ggcagtgggc gataaagtga
    15181 ccgataaaga ttatttcggt actggcctcg gcatcgcggt acgtcagggc aacactgaac
    15241 tgcagcagaa actcaacact gcgctggaaa aagtgaagaa agatggcact tacgaaacca
    15301 tctacaacaa atggttccag aagtaattcc tgatgaatga attttttcct ttagcaagcg
    15361 ccgccgggat gaccgtcggc cttgccgttt gtgcattgat tgtcgggctg gcgctggcga
    15421 tgttctttgc ggtatgggag tcagcgaaat ggcgtcctgt cgcgtgggca ggttcagcgc
    15481 tggtaaccat tctgcgtggc ctgccagaaa ttctggtagt gctgtttatc tattttggct
    15541 cctcgcagtt actgctgacg ctttcggatg gcttcactat caatcttggg ttcgtgcaga
    15601 tcccggtgca gatggacatt gagaacttcg acgtgagtcc gttcctttgt ggtgtcatcg
    15661 ctctgtcact gctgtatgcc gcctatgcct cgcaaacgct gcgcggcgcg ttgaaagctg
    15721 taccggtggg ccagtgggaa tctggtcagg cgctggggct gtcgaaatcg gctatctttt
    15781 tccgtctggt gatgccgcag atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc
    15841 tggtgctgct gaaagatacc gcgctggtca gtttgattag tgtgaatgat ttaatgttac
    15901 aaaccaaaag cattgctact cgtacccagg aaccatttac ctggtacatt gtggcggcgg
    15961 cgatttacct ggtgatcacc ctgctcagtc agtacattct caaacgcatt gacctgcgcg
    16021 cgacacgttt tgagcggagg cccagctaat gtttgagtat ttacccgagc tgatgaaagg
    16081 gctgcacacc agcctgacgc taacctttgc ctcgctgatt gtggcactga ttctggcatt
    16141 gatttttacc atcatcctga cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta
    16201 tatcacgctg tttaccggta cgccgctgct ggtgcagatc ttcctgattt attacgggcc
    16261 gggccagttt ccgacgttgc aggagtatcc ggcactgtgg catttgttgt cagaaccgtg
    16321 gttatgtgcg ctgattgcgt tatcgctgaa cagtgcggcg tataccacgc agctgtttta
    16381 cggtgcaatt cgtgcgatcc cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag
    16441 caaaaaagat acgctggcga tcctgctgcc gtatgccttt aaacgctcgc tctcttctta
    16501 ttccaacgaa gtggtgctgg tgttcaaaag tacctctctg gcttacacca ttacgctgat
    16561 ggaagtgatg ggatacagcc agttgttgta cggacgcacc tacgatgtaa tggtgttcgg
    16621 tgcggcaggg attatttacc tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat
    16681 cgagcgcaaa gcactggcat tcgaacggcg gaattaatgt agctgcgtac attcgccggg
    16741 ggggtttgta ggtctgataa gacgcgccag cgtcgcatca gacatcagca cggtgcctgt
    16801 gccggatgcg gcgtggacgc cttatccggc ctacatagtg cataaattct tataatgaag
    16861 acggacaact cactaagtta tccgtctttt tttatttcat ttaaattatt taatcatgtt
    16921 tattgcatat aaattcactt ggtggcattg ttatcccatg ccgcagacac ggccaaaaat
    16981 cataagattg acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc
    17041 ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta
    17101 tccacccttt gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc
    17161 aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt actaatcacg cgttcgacag
    17221 cctgatcccg tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac
    17281 cccggagcgt agcaagcagg tttcgtttac cacaccctac tatgaaaact cagccgtcgt
    17341 gattgccaaa aaagatacct acaaaacgtt tgccgacctg aaaggcaaac gcattgggat
    17401 ggaaaacggt acgacgcacc agaaatatat tcaggatcag cacccggaag tgaaaactgt
    17461 ctcctatgac agttatcaga atgcctttat cgatctgaaa aatggtcgta ttgatggggt
    17521 atttggtgac accgcggtgg taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc
    17581 gactgaaaaa gtgaccgatc cgcaatactt cggcaccggc ctgggtatcg ctgtacgtcc
    17641 ggataacaaa gctctgctgg aaaaactgaa taacgcgctg gcagcaatta aagctgacgg
    17701 tacatatcag aaaatcaatg accagtggtt tccgcagtaa ttgagactga tgacaaaagt
    17761 aagattgcct gatgcgctgc gcttatccgg cccacatcaa ctctgcaatg tattgattta
    17821 gcatgatttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcacc
    17881 tgatcagcaa tctgaagccg cacctgtggc ttttttattg cttcaccagc agcgtcagca
    17941 cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcggtaac
    18001 ctggcagttc gcggatatct ttcgccatgg tttgggtgtt acagctggag tagataataa
    18061 aacgcggtgc catcgttgaa agataatcac acagcggttt accaatgccg cggcgcggcg
    18121 ggttaaccag caccagctcc ggcacctccc cctgagcagt ggcaaactga gtggagtcca
    18181 gcgcctgaaa ttgtaaacga gttaagccca gttcagcggc tgactgcttt gcacaggcaa
    18241 tggcctctgg cgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac
    18301 caaagccccc cacaccgcag aacagatccc acatatgttt aaccggcagc tgtcgtaccc
    18361 agtcgcgcgc ggtggcgtac aactggctgg cgaccgccgg attagtctgg aagaaacttt
    18421 gcggacggat ccacagcggt acgtcattaa aacgctccgc cagtgcctgt tgttcggtca
    18481 ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa
    18541 taactttcag ctgcggtagt tgttcgtgta accacggcag cgccttacgc agttgcgcca
    18601 gcttggtatc agaacgcagt acaaagcgca gcatcatgcc gccatcactc tggctttcag
    18661 tcagcagaat gtatttcagt tcgccacgct tacgcgccac gttgtagggc gttaaccccg
    18721 ctcgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaatgaggct ggataaagcg
    18781 ggcagtcaca aaggtcttcc ggcgtgccat cgcgatgcag cataccgagc agtggttttt
    18841 caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc gggcctgaca
    18901 ccggcgcgca ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggttt
    18961 tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg
    19021 cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca
    19081 cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg
    19141 agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg
    19201 ggaatgctga acagctccgg ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg
    19261 gcggtgattt gtgtcagcag atatagccag cgcgcccagc gacgcccttt caccagtgaa
    19321 aacgcacacc agatctcaat gaaaaccagc accagactgc ttaaaaagac cagcgttaaa
    19381 ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg
    19441 aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc
    19501 agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt
    19561 tcgtcttcca tgaaaacaat gccgcgacat gcgcggcatt atgtagccag gttggcaaat
    19621 tttagtgtct tcagccacgt cttgcacgtt ggatgtcgcg tatccgctgt ttttctgcgc
    19681 gcgccatcag ataccaggcg ataaatccga caattccgac cgcgccgagg atcaatgttg
    19741 ccaaggcgtt gatttccgga ttcaccccca tccgcacgct ggaaaagacc agcatcggta
    19801 acgtggtggc tcccggcccg gaaacaaagc tggcgatcac cagatcatca agcgacaaag
    19861 taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga
    19921 aaaacacttt cagcggcgtc gcaccgagat ccatcgcggc ttcttctatc gagcgatcca
    19981 gttcccgcag acgcgacgaa ataacgaccg ccacataagc cgtacagaaa gtgacatgcg
    20041 ccagccagat ggtaagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga
    20101 cgaataataa caacagcgac aagcccgtga tgacatctgg cattaccagc ggcgcggtga
    20161 tcataaaggc aaagccattt gatccgcgaa acctgccaaa gcgcaccagc accaccgccg
    20221 caatagtccc gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc aggcttaaac
    20281 caaccgcact catcatcgcg tcatcgcgca ataactcacc ataccagcgc gttgaccagc
    20341 cggcccacac cgtcaccagc ttcgagctgt taaacgaata gataaccagc atcagcattg
    20401 gcgcgtagag aaaggtaaag cccagcagca aaatcacaat ccgctagggc gaacgaacta
    20461 ccggtaaatt attcatccgt gttctcccac gcttttttgc tggtgtttgt ggaaccacat
    20521 tatcggcaca attagcagca gcaacatgat gatcgctacc gccgaggcca ccggccagtc
    20581 gcggttattg aaaaactctt gccatagcac gcgcccgatc atgatgctgt ccgggccacc
    20641 gagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc
    20701 aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc
    20761 accgagatcc agcgctgctt ccaccagcga ataatcaata cgaatcaacg cggtataaat
    20821 cggcagcacc ataaacggca cgtaggcgta aacaatgcca atataaactg ccagattggt
    20881 atgaagaatg gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac
    20941 accgttgttt tttaatattc ccatccaggc ataaacgcgg atcagaaacg aggtccacga
    21001 cggcaggatc accagtagta ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc
    21061 ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag
    21121 cgactggaga taagcatcga aatagagcgg atcgtcggtc agttgcagaa aattaccgag
    21181 attaagagtg atggacagtt gcccgtcagc ccactccatc agttcggtat aaggtggaat
    21241 agcgcgcgcc atctccgcca ggcttatttt aaagacaatc agaaatggca gcagaaacag
    21301 caagatcaac cagatatatg gcaacgcaat gaccagtttg cgcccatgct tcatttgcag
    21361 ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt
    21421 attcatcttt gctccttaaa ccgtcagcac cacacagctg tccacttccc agcacaaacg
    21481 cacttcgtcg ccccaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc
    21541 gctaatcatc tgcccacttt tcagacgaac gtgatacacc gaaagatcgc cgagataggc
    21601 aatgtgtatc acctccccca ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag
    21661 catgattttt tccgggcgca gcgccacatg taccggcacg ttatcgacca ccgaggcatc
    21721 cgcgtcgact ttcagtggat gcaccagccc cggcgaatca agcaccaggc catcttcctg
    21781 acgctctttg agtacgcctt caaagacgtt taccgagcca ataaattcgg cgctatagcg
    21841 ggtagtcgga tgctcgtaga tctcttccgg ttcgccaatc tggacaaatt tcccacgatt
    21901 cataatggcg atgcgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac
    21961 acaagtcaca ccgacgcgtt ccagaatatc caccacttca agctgcatcc ggtcacgcag
    22021 ctttttatcc agcgcgccca tcggctcgtc gagcagtaat agtttcgggc gcttggcaag
    22081 gcttcgggcc agggccacac gctgtcgctg accaccagaa agctgatgcg gtttacgttt
    22141 ggcgaactcc tgcatgtgca ccaggccgag catctcattg acccgactgg aaatttccgc
    22201 tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg
    22261 aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg gaacctgtga
    22321 caaatcgacg ccatcaagca ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat
    22381 acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt
    22441 gtagatggtc aggctgacat catccaccgc atgttgacca tcgtaggatt tggtgaggtt
    22501 gcggatttct aatagcggcg tcagcgcctt acgggttttc gcctgcgggc gagggatagc
    22561 gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt
    22621 gccggcgggt gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt ccgctcttca
    22681 ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact ttcagagtga
    22741 acagcttcgc acgaacatcc gcaggcggat aaatacccgg gttctcacgg acttccgcac
    22801 tcaccagcgg cgtggctgct ttattggcgt tggcatagaa cacatggtcg gaaatatgcg
    22861 ctactacatc cgggcgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg
    22921 catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga
    22981 acgagacatt cactccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac
    23041 ctgcccagcc gatagcgacg caaatatcgc cgtttgccag gtcgttaatg tattgagatg
    23101 aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggttgcc ggtccggtgt
    23161 aatcatccgc tttagtgctg ttgggatctt tgccgagata attcaacacg gtagcaaaaa
    23221 cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag
    23281 gtttgaggat caagtcccag ctatcgacgg gcgcgttttc gcccagcaca gctttaactt
    23341 tatcaacgtt atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt
    23401 cgggatcgtg tttggcgacc agcttcagca gctccgggtc gagattcttc cactccggca
    23461 atttgctttt gtccagcggc tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg
    23521 cagatggaac caccaagtca aagccggtgc tacccgccat taatttgcct tccagtactt
    23581 cgttagagtc gaaaacatcg tagacgactt taataccggt ttctttttca aaattggcca
    23641 ccgtgtcagg ggcgatataa tcagaccagt tataaatgtg gagtgttttt tgttcagccg
    23701 cgagcgtgcc gacagagacg gccatcagag cacccgcaac cagacccgat agccattttt
    23761 tatttaaggc ggtcacatct ctttccttct gaaagttcgt taacaaacga acaaaagttg
    23821 tcacactgaa agatatgcaa taaacgtgca tattttttta tcggcgcggt gtaagaaaag
    23881 agaactctct cccggccagc actgctcgct atataaaagt atcgcaagaa taactttagc
    23941 cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt tatgcgtatt
    24001 tgctctatgt gataaagcca aatcgacaca agagaagtga aataatcttt aagaaaaggt
    24061 taaaacagca gataattttg cagcacgcag tcactgcggc agcgactgcg tgctcgaaag
    24121 gaattatcag tggagaaaat agttttcggt aacgttgttt tgcccttcat cgtcagtcgg
    24181 taacagcagt tgatgggcat tagcttcaag aatgaccatc gaaatctgct cttcgctctg
    24241 acgcacgaac catgcaaact gctcataggt tacgccctgc ataacggata aagactggca
    24301 aaccaccagc tttggcagat tatcatcctg catatcaaga aatgctttca cggtcagcga
    24361 actggcattg atggcagata aatccgccgc cagcggcaat acggctgaag gtcggacttc
    24421 cgccatggca gaaaacagga tcgtgttatc gatcagatcg attttggcat caaagacacc
    24481 gtcgaaattc tgcatatggg gcagatgcag agcctgacag ttatcacatt caaaaaaact
    24541 catccccagg tcatcgagcc attgacgcag cgtatccaga ccaggaacga ccagcgatgt
    24601 cataattttg tacaacctct ttcgataaaa agaccggcac agcttacgca aaaagcgcag
    24661 gcaaaaccat gatcagtaat gtgattgcga ctaaccaccc gttttcaggc aatattctgt
    24721 cgtagcgtgg cgttcgatcc agcggatcat tttacccgcg atatcgatac cggtggtttt
    24781 ttctattcct tccagccccg gcgacgcatt cacttccatc accaacggcc cgcgattagc
    24841 acgcagaata tccacaccag caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat
    24901 ttcacgctcc tgtggtgtga tactggcgac gcttgccgcg ccgccacgat gcaaattgga
    24961 acgaaaatag ccctctttcg cccaccgttc aatcgcagcg accacttcat caccaacaac
    25021 cagacagcgg atatcgcacc cttgcgcctc tttgatatat tcctgcacca gaatatgcgc
    25081 gttcagaccg cggaaagcgt caatcacgct ttccgccgcc tgacgagtct ccgccagcac
    25141 gacgccaatt ccctgcgtgc cttcaaccaa cttgaccacc agcggcgcac caccgaccat
    25201 gtcgattaaa tcgctggtat catccggcga atgcgcaatg cccgtgacag gcaggtcgat
    25261 gccctgacgc gccagcagtt gcatggaacg caatttgtca cgcgcccggg caatggcgac
    25321 cgactcattg agcggatagc tccccagcat ctcgaactgg cgcagtgccg ccgtcccata
    25381 aaaggtaatg gcggtgccaa tacgcgggat cactgcgtca aaatggggta acttgcggcc
    25441 tttgtagtga atagaagacg ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag
    25501 aatttcaacc aggtgaccgc gctgtatagc ggcttcacgc agccgcttac acgaatagag
    25561 cgttccatcc cgggacaata tggcaatttt caccctgcac ctctctgtca gacctggtca
    25621 aaagcccgcg tatcatacat cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg
    25681 caaataatcc agaataaatg ggcggctttc tttaataatt gttcggcgga tatgatcgct
    25741 ccaggtatcc cggcgattat tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc
    25801 atactgcgcc agtgcgtctt tatccagcgg ttgatagctg ttttcatgca ccaaaatgga
    25861 ggccggtaaa cgcggcttga gatccggatt atccgcaggc cagccaaggc acagcccaaa
    25921 cagcggcaga acatgctgcg gtaat
//
LOCUS       DADKXM010000045        25873 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.045, whole genome shotgun sequence.
ACCESSION   DADKXM010000045 DADKXM010000000
VERSION     DADKXM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.045"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            309..473
                     /locus_tag="JQR05_001848"
     CDS             309..473
                     /locus_tag="JQR05_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY5324033.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            476..643
                     /locus_tag="JQR05_001849"
     CDS             476..643
                     /locus_tag="JQR05_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324034.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            757..852
                     /locus_tag="JQR05_001850"
     CDS             757..852
                     /locus_tag="JQR05_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324035.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            1016..3691
                     /gene="acnA"
                     /locus_tag="JQR05_001851"
     CDS             1016..3691
                     /gene="acnA"
                     /locus_tag="JQR05_001851"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY5324036.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYVLAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3755..4345)
                     /gene="ribA"
                     /locus_tag="JQR05_001852"
     CDS             complement(3755..4345)
                     /gene="ribA"
                     /locus_tag="JQR05_001852"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY5324037.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHIALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4515..5279
                     /gene="pgpB"
                     /locus_tag="JQR05_001853"
     CDS             4515..5279
                     /gene="pgpB"
                     /locus_tag="JQR05_001853"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY5324038.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5428..5736
                     /gene="lapA"
                     /locus_tag="JQR05_001854"
     CDS             5428..5736
                     /gene="lapA"
                     /locus_tag="JQR05_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY5324039.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5743..6912
                     /gene="lapB"
                     /locus_tag="JQR05_001855"
     CDS             5743..6912
                     /gene="lapB"
                     /locus_tag="JQR05_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY5324040.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7105..7842
                     /gene="pyrF"
                     /locus_tag="JQR05_001856"
     CDS             7105..7842
                     /gene="pyrF"
                     /locus_tag="JQR05_001856"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY5324041.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7842..8168
                     /gene="yciH"
                     /locus_tag="JQR05_001857"
     CDS             7842..8168
                     /gene="yciH"
                     /locus_tag="JQR05_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY5324042.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8294..8512)
                     /gene="osmB"
                     /locus_tag="JQR05_001858"
     CDS             complement(8294..8512)
                     /gene="osmB"
                     /locus_tag="JQR05_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY5324043.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8781..9530)
                     /locus_tag="JQR05_001859"
     CDS             complement(8781..9530)
                     /locus_tag="JQR05_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5324044.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9620..9793)
                     /locus_tag="JQR05_001860"
     CDS             complement(9620..9793)
                     /locus_tag="JQR05_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324045.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(9941..11926)
                     /gene="pdeR"
                     /locus_tag="JQR05_001861"
                     /pseudo
     CDS             complement(9941..11926)
                     /gene="pdeR"
                     /locus_tag="JQR05_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            11980..12082
                     /locus_tag="JQR05_001862"
                     /pseudo
     CDS             11980..12082
                     /locus_tag="JQR05_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12160..14094)
                     /gene="rnb"
                     /locus_tag="JQR05_001863"
     CDS             complement(12160..14094)
                     /gene="rnb"
                     /locus_tag="JQR05_001863"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY5324046.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCGQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(14162..15269)
                     /locus_tag="JQR05_001864"
                     /pseudo
     CDS             complement(14162..15269)
                     /locus_tag="JQR05_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            complement(15413..16201)
                     /gene="fabI"
                     /locus_tag="JQR05_001865"
     CDS             complement(15413..16201)
                     /gene="fabI"
                     /locus_tag="JQR05_001865"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY5324047.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(16569..16922)
                     /locus_tag="JQR05_001866"
     CDS             complement(16569..16922)
                     /locus_tag="JQR05_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY5324048.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(16990..17796)
                     /gene="sapF"
                     /locus_tag="JQR05_001867"
     CDS             complement(16990..17796)
                     /gene="sapF"
                     /locus_tag="JQR05_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY5324049.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(17798..18790)
                     /gene="sapD"
                     /locus_tag="JQR05_001868"
     CDS             complement(17798..18790)
                     /gene="sapD"
                     /locus_tag="JQR05_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY5324050.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLMGAKNHLYACHFPLNMEKE"
     gene            complement(18790..19680)
                     /gene="sapC"
                     /locus_tag="JQR05_001869"
     CDS             complement(18790..19680)
                     /gene="sapC"
                     /locus_tag="JQR05_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY5324051.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGKVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(19667..20632)
                     /gene="sapB"
                     /locus_tag="JQR05_001870"
     CDS             complement(19667..20632)
                     /gene="sapB"
                     /locus_tag="JQR05_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY5324052.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(20629..22272)
                     /gene="sapA"
                     /locus_tag="JQR05_001871"
     CDS             complement(20629..22272)
                     /gene="sapA"
                     /locus_tag="JQR05_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY5324053.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(22585..22830)
                     /locus_tag="JQR05_001872"
     CDS             complement(22585..22830)
                     /locus_tag="JQR05_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY5324054.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(22964..24349)
                     /gene="puuP"
                     /locus_tag="JQR05_001873"
     CDS             complement(22964..24349)
                     /gene="puuP"
                     /locus_tag="JQR05_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY5324055.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFIV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(24339..24503)
                     /locus_tag="JQR05_001874"
     CDS             complement(24339..24503)
                     /locus_tag="JQR05_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324056.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(24652..25842)
                     /locus_tag="JQR05_001875"
     CDS             complement(24652..25842)
                     /locus_tag="JQR05_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY5324057.1"
                     /translation="MDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM
                     LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGDLQPP
                     CAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLY
                     HTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGEN
                     VLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNR
                     TVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLE
                     QEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWM
                     LKNA"
BASE COUNT         6523 a         6490 c         6644 g         6216 t
ORIGIN      
        1 tgccggaaaa ataatttctt gcgttatttt cggcaccttt tatgtagcga aggtgccgga
       61 atatattctc ttttgttact tatttctata ccgtgcacaa tatttttatc cctcatattt
      121 atagggtaaa ttacctgtaa agtagtgtct aattacccgc ctcgcaaatt ccagttttca
      181 aatatctatt tcaggtcaaa agattgaatt attcactgga gacgattcgt aaattcgctg
      241 gatttttgac taaagtttct ttaggatttg tctcatcgta tgattaatta caccaatcat
      301 tcaacgtctt gctgataagt gatggcgatt atatgaggtt agcaatgcct tctggaaatc
      361 aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg gctggcggtt tttcttggtt
      421 ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa agtgtgggga taactatggt
      481 cggtcaggag caactggagt cgtcaccatt atgccagcat agtgacaatg aaccagaaac
      541 gaaacgggaa tgttccgtcg ttattccaga cgactggcaa ctaacatcgc agcagcaagc
      601 ctttatagaa ctgtttgctg aagatgatca gccgaaacaa taattatcat cattcttatt
      661 acccattttt aatgaattaa agggctttta atacaccgca gcaataacag cttgagttat
      721 ctcaacacaa aataataacc gttaagggtg tagcctatga tcaacacaaa tatgaaatat
      781 tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct gggccgaact cctctggatc
      841 attactcact gatccttgac cccgctgcgg cggggttgtc atttgctttg ccacaaggtt
      901 tctcctcttt tatcaatttg ggttgttatc aaatcgttac gcgatgtttg tgttatcttt
      961 aatattcacc ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc
     1021 gtcaacccta cgagaagcca gtaaggacac gttgcaggcc aaagataaaa cttaccacta
     1081 ctacagcctg ccgcttgctg ctaaatcact gggcgatatc acccgtctac ccaagtcact
     1141 caaagttttg ctcgaaaatc tgctgcgctg gcaggatggt aactcagtta ccgaagagga
     1201 tatccacgca ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg
     1261 cccggcaagg gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc
     1321 aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc
     1381 ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt
     1441 tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg
     1501 gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca tttgccatca
     1561 ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat
     1621 tgcttatccg gatacactcg ttggtactga ctcgcacacc actatgatca acggccttgg
     1681 cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc gccatgttag gccagccggt
     1741 ttccatgctt atcccggacg tagtgggctt taaacttacc ggaaaattac gtgaaggtat
     1801 taccgccaca gacctggttc tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg
     1861 gaaattcgtc gaattttatg gtgatggtct ggattcacta ccgttggcgg atcgcgccac
     1921 cattgccaat atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgcagt
     1981 aaccctcgat tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa
     2041 atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt ttaccagtat
     2101 gttagaactg gatatgaatg acgttgaagc gagcctggca gggccaaaac gcccacagga
     2161 tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tggaagtgaa
     2221 tgccacgcat aaagatcgcc tgccagtcga ttatgttatg aacggacatc agtatcagtt
     2281 acctgatggc gctgtggtca ttgctgcgat aacctcgtgc accaacacct ctaacccaag
     2341 tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta actcttggcc tcaagcggca
     2401 accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc gtttctgatt atctggcaaa
     2461 agcgaaactg acaccgtatc tcgacgaact ggggtttaac cttgtgggat acggttgtac
     2521 cacctgtatt ggtaactctg ggccgctgcc cgatcctatc gaaacagcaa tcaaaaaagg
     2581 cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc
     2641 gctggttaaa actaactggc tggcctcgcc gccgctggtg gttgcctatg tgctggcggg
     2701 aaatatgaat atcaacctgg cttctgagcc tatcggccat gatcgcaaag gcgatccggt
     2761 ttatctgaaa gatatctggc catcggcaca agaaattgcc cgtgcggtag aacaagtctc
     2821 cacagaaatg ttccgcaaag agtacgcaga agtttttgaa ggcacagcag agtggaagga
     2881 aattaacgtc acacgatccg atacctacgg ttggcaggag gactcaacct atattcgctt
     2941 atcgcctttc tttgatgaaa tgcaggcaac accagcacca gtggaagata ttcacggtgc
     3001 gcggatcctc gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag
     3061 tattaagccc gacagcccag cgggtcgata tctacaaggt cggggtgttg agcgaaaaga
     3121 ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc
     3181 caatattcgc atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt
     3241 acctgacagc gacgtagtct ctatttatga tgctgcgatg cgctataagc aggagcaaac
     3301 gccgctggcg gtgattgccg ggaaagagta tggatcaggc tcaagccgtg actgggcggc
     3361 aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc gaatcgtttg aacgaattca
     3421 ccgttcgaat ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtgacgcg
     3481 taaaacgtta gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaatctaca
     3541 acctggcgcg acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc
     3601 ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac taccagaacg acggcatttt
     3661 gcattatgtc attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt
     3721 ccggcaagca aatgaattac acaatgcaag agggttattt gttcagcaaa tggcccattt
     3781 tctcggcttt ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta
     3841 cgcgttcaac aatattaatc cctgcttcgg tcagaatttc gacttttttc gggttattgg
     3901 ttaacaagcg gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt
     3961 cgcgctcatc agcggcgaag cctaactggt ggttagcctc tacggtatcg taaccttgat
     4021 cctgcagtgc gtaagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt
     4081 gatacagcaa aataccacgg ccttcctcgg caatttgcgt caatgccgct tcgagctgga
     4141 agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc
     4201 gcgcaagtac cggggtatgc ccggaaatat cgccatagac tagcgcgata tgatcgtgtc
     4261 cggttgccag ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg
     4321 cttctgccac acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct
     4381 tattttgcca taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg
     4441 gcacaaatct gtcaattttt cttggaactg gcattttcag ttatgattgt gggacttatc
     4501 aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc agtgggagct gcactattgc
     4561 ttgtcatgcc agtagccgta tggatttctg gctggcgttg gcaacctgga gaacaaagtt
     4621 ggctactaaa agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta
     4681 cacatttgat tttgttcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct
     4741 ttgtattatt tgccattctg gcggcggcaa tccttgttgg acaaggcgtt aaatcctgga
     4801 tcaaagacaa agtccaggaa ccacgacctt ttgttatctg gttggaaaaa acacatcata
     4861 ttccggttga tgagttctac actttaaagc gagcagaacg cggaaatcta gtaaaagaac
     4921 agttggctga agagaaaaat attccacaat atttgcgttc acactggcag aaagagacgg
     4981 ggtttgcctt tccttccggt cacacgatgt ttgctgccag ttgggcactg ctggccgttg
     5041 gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc tgggcaacgg
     5101 gagtcatggg aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta
     5161 cgttgatttc gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga atttgtgggc
     5221 cattaacacc acctgcggaa gaaaatcgcg aaattgcaca acgagaacaa gaaagttaac
     5281 gctggttgat tttccgaatt taggccttaa atcatcaaca atgcgcgtgg atgccatttc
     5341 gcagacggcg cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt
     5401 cgctctaacc acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta
     5461 gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg atcaacaggt gacgtttaat
     5521 tatctgttag cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgca
     5581 gggtttgcta tcggttggtt gatttgtggc ctgttctggc tgcgagttcg tgtttccctg
     5641 gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg
     5701 gctgtagtgc cgcactcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt
     5761 ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa
     5821 aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg
     5881 cttagtaatc aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggataca
     5941 ggcaccgttg aagcccacct tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat
     6001 cgcgctattc gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt
     6061 ctgttggcga ttcaacaact ggggcgtgat tacatggccg ccgggttata cgaccgcgcg
     6121 gaagacatgt tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag
     6181 ttgctacaaa tctaccaggc taccagtgag tggcagaaag caattgatgt tgccgaacgc
     6241 ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg cccatttcta ctgtgagtta
     6301 gccctgcagc atatggccag cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg
     6361 gcggcggcag ataaaaacag cgcccgcgta tccatcatga tgggacgcgt gtttatggcg
     6421 aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg tcatatccca ggacagagaa
     6481 ctggtcagcg aaacgctgga aatgttgcaa acctgctatc agcagttggg taaaactgcc
     6541 gaatgggcag aattcctgca acgcgcggtg gaagagaaca ccggtgccga tgctgaattg
     6601 atgcttgctg atatcatcga agcgcgcgac ggtagtgagg ctgcacaggt ctatattaca
     6661 cgccagcttc agcgtcatcc gaccatgcgt gtattccata agctaatgga ttaccactta
     6721 aatgaagcgg aagaagggcg cgccaaagag agtctgatgg tgctgcgtga catggttggc
     6781 gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat gtggttttac cgcatacacc
     6841 ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat tcgcggtctt
     6901 gatggcctgt aatttttaaa aaaatccgac tttagttaca acatactagt tattgatgtc
     6961 ccattgtgct ccggcaacga cggagcagaa aaagcctgct aggggaaaaa tcgcaactgt
     7021 taatttttta tttccacggg tagaatgctc gccgtttacc tgtttcgcgc cacttccggt
     7081 gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt
     7141 acgaattctc ctgtggttgt tgcccttgat tatcataatt gtgatgacgc gctgtccttt
     7201 gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg gtaaagagat gtttacattg
     7261 tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg
     7321 aaattccacg atatccccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc
     7381 gtgtggatgg tgaatgttca tgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag
     7441 gcactggttc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt gttgaccagc
     7501 atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa
     7561 cgtctggcag cactgacgca aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa
     7621 gctgtgcgct ttaaacaggt attcggtcag gagttcaaac tggttacacc gggcattcgt
     7681 ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg
     7741 gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcag
     7801 acactgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc
     7861 gtctggtcta ctcaacggaa accgggcgta ttgatgagcc caaagcggcc ccagtacgcc
     7921 ctaaaggcga tggagcggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg
     7981 tttgcctgat taccggtatc gatctcgatg atgccgaact gacaaaactt gcagcggaac
     8041 tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg agttattgaa atccagggcg
     8101 ataagcgtga tttattaaaa tcactacttg aagcgaaagg gatgaaggta aaactcgcag
     8161 gcggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat
     8221 tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga
     8281 catttattta cgattattta ccgacctggt gaccaataac accaccgaca gctgcaccac
     8341 ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg
     8401 cgccgattgc ggtgttgcgg tcccgtttag accagttaga acaggcactc agagacattg
     8461 ccaaagtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct
     8521 ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc
     8581 ctggtgaaat tattccgtga aatctgctcg cgcgcagggt tatatcacgc aggtgataat
     8641 tacttcctgt tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa
     8701 tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa ctgcaaatca
     8761 tccgtagaga agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt actgtgctca
     8821 agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca
     8881 acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc
     8941 gcttcgcact ctttttccag cacggcattg accacatcgg tgcgcatcat atcgcgaccg
     9001 gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc
     9061 tgctggatgc actggcgtgt caaagggcca accatacttt cgcttttttt ctggtacacg
     9121 ccaccgagca aaatgacttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg
     9181 accgtgatga tagtgacatt tttcttctgc tcccccagag tccgggcgag cagggcattg
     9241 ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg
     9301 gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca
     9361 agcgaaactg caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga
     9421 tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc
     9481 tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattcat cgctgtaatt
     9541 cttataacgt tataatacta aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga
     9601 aaacctgaag gcacgattgt taaatctccc acggtgtctt cgggatgtct ggcgtagatt
     9661 cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc gcagagtggt
     9721 aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg cgctgggcat
     9781 caaattcaga catcaaggac atccttatag caaaaggggg aaaatgaata atgcaaaaat
     9841 tgccgctaaa agagaagtgt ttaacagcaa cggctaatta tcatccagga atacgatata
     9901 taatgacggg atatagcgct aagtatatat attcatctac ttatgcgcgc ttcagatagc
     9961 atttatacca gcgttcgaag gcgacggcgg gcatcggttt ggcaaacaaa aatccttgcc
    10021 gctcattgat cccgttcttg gttaaaaaag catcttcctt tgcactctct acaccttcgg
    10081 cgatcacctg aagattcaat gcctgggcca cagcgacgat cgcccggacc agtgactgcg
    10141 agacaggttg tttgtgaata tctcgaacaa aaacctggtc aagtttgatg gcatcgatcg
    10201 gaaagcgcgc cagttgcgaa agtgaagagt agccggtacc aaaatcatcc agatgcactt
    10261 gcgcacctag ttggctaaat tgttgaataa cagacagtgc cagttcatca ttctcaatca
    10321 gacaactctc tgtcagttca acatctatag ggcagtattc aaaattgagt tcctggagaa
    10381 cctgtttcag ggcggtgaaa atggtttgat cggcgagctg acgtgcagaa atatttaccg
    10441 ccactcgcag gtttatgcct ttatcccgcc actttgccac ctggcgtacg acatcgagaa
    10501 tcacccaacg gcctaaaggc acaattagcc ctgactcttc ggcgtaggaa atgaagtcca
    10561 acggtggaat caacccacgt tcaggtgact gccaacgtac tagtgcttcc agactgcgca
    10621 cttcgccacg ctaggtgatt ttcggttgat agtgaataac caactgatcg ttttccagtg
    10681 ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc atttctggag
    10741 taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt
    10801 gacgaataat agtcgcgctg tccgaaccat gttcgggaga gagcgaaata cctactgaac
    10861 agctggtata aacttcaatt aaaccaatac gaaaggggag gcgtaagcgg gtcaaaattc
    10921 gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc agaaactcat
    10981 cgccacctgg acgcgccaac acctggtcat gttcgagaca gcttaaaata gccaatgaca
    11041 cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc tttttaaaat
    11101 tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta atagcgtgat
    11161 cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg gtatttgcca
    11221 gaatacgcag tcgctcctga gtgcggcgct cttcggtaat gtcggtaccg gaacagatta
    11281 aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac agaaacagcc
    11341 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    11401 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    11461 cgctttgccc aatgacgtcg tgttctttca accctgtgta atcttcacat aaccgattga
    11521 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    11581 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt gtgtcatgcc
    11641 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    11701 aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    11761 ccctgatgcg agcagcctgt tcggcagata actgcaatgt acgatcaggt tctgtggttt
    11821 cggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    11881 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    11941 ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    12001 gcggctaaac gcattgtttt ttatatattt atttgtaatc cagttttgga aaaacgccag
    12061 ttttcaaacg aaagtcagtt aaaaaatctg cctggatata acgaaggtag agcggggaaa
    12121 taaacggccc atccatgagg aatgggccgt gaaaggagat tacgcgaccg ggcgcgcaat
    12181 aatgctgcgg gtttccatgc ggacttcggc aatggtgacg tcaataacgt cagttacttt
    12241 gtaaaccgtt tcacctttaa tttgtacagt gccgttttcc tggccgcaaa ccagttcatc
    12301 gcgcacagcg tgtaagaaag gtgccggaat aaaggcgata gcgccgttat caaccaaacg
    12361 cacacgcatg ccgccacggc tgatatcgac aatttccgcc gcgaaacggg tgccggtccc
    12421 ggctttatct ttcaggaagc gtgcgtataa ccagtcgcca acatcacgtt ctgccatccg
    12481 gttgagacgg cgacgttcgg ccatttggac agtgatttca tcctgcggac gcgtcgcagt
    12541 ttcgcctttg ataaccgctt tcagcagacg gtggttgatc atgtcgccat atttacggat
    12601 cggcgaagtc caggtggcgt atgcttccag accgaggcca aagtgaggac cgggttcagt
    12661 gctaatttca gcaaatgact ggaagcgacg aatgcggctg tcgaggaaac cagttggttg
    12721 cgcatccagt tcacgacgca gtttgcagaa accgtccagc gtgagcactt cttcggcatc
    12781 gacatgcaga ccgtgcgttt tcagcaacgc tgccagcgcg tcggcattcg ccggatcaaa
    12841 gcccatatgc acgttataga tgccaaaacc gagcttatcg cgcagtacgc gggccgcaca
    12901 aatgttagcg gcaatcatcg cttcttcgac gatacggttg gcaatgcgac gaggctcggc
    12961 gacgatatcc agcacttcac ctttttcacc gagaataaag cggtaatccg ggcgatcttt
    13021 aaacaccagt gcgtggttat gacgccactc gccgcggcgt tggcaaattt gcgctagcaa
    13081 acggacttgt tcggcaattg cttcactttc aggcttccag tcaccggtat tttccagcca
    13141 gtcagaaacc tggtcataca ccagcttcgc tttggattcg atggtggcgg caaagaattc
    13201 gatattatct tcaatggtgc catcagcgga gagcgtcatg cggcatgcca gtaccgggcg
    13261 gacttcattg gcgcgcagtg agcagagatc gtcagaaagc tcgcgaggca gcatagggat
    13321 gttgaagcca ggcagatagt tggtgaatgc gcgaattttc gcggctttgt ccagcttgct
    13381 gccttcagca atccacgcgg ttggatcggc aatcgccaca atcagctgaa gtttgtcatc
    13441 cggcagcgcc ttagcgaaaa gggcgtcatc catatcttca gtgctggcac tgtcgatggt
    13501 gacaaaatcc agcgaggtca gatcttcacg aaccagacct tcatcgagca tttcggtagc
    13561 gacgccgtct ggtgcttctt tttccagatt atggcgcgca agggtaaccc accacggtac
    13621 aaagtggtcg tcaccaaaag tgatgtattg tgtcagttca gcatagaaag aacgatcgcc
    13681 tttcagcgga tgacggcgca tttcggcaac cgcccagtcg ccttctttaa actcgtggtt
    13741 caggccacgg gctgcgcggc aaggaatggc gtcttttaaa agtggatgat caggaacgat
    13801 ggtcagacgg tcatttttgc cctgaacttt acccacgaaa cgagtcagga acggttcaac
    13861 cagttcttct ggctctgcgg attcacgttc tttttcactg tggatcaccg cgataattcg
    13921 gtcgccatgc atgacttttt tcatctgcgg cggcggaatg aaataacttt tttgcgcgtc
    13981 gacttccagg aagccaaagc ctttttctgt ggcttttacc accccttcag cgcgtggcgt
    14041 ctgggaatgc agttgctgtt taagctgcgc tagcagcggg ttgtcctgaa acataattgt
    14101 ctattttggt ggccattaga gcggctgaca gttttacgcg aatctgtctg acgcggcaag
    14161 gttaatatgt ctcacccaac gcgattttta agcgatttat ccagccacac agcccgctcc
    14221 ataccagcag attaataatc tgcgttgatg atttttcgtg ttcgagtaaa gggctgaact
    14281 gggcggcgct aaagcgatcg ggtgctcggg tcagccattg tatcgcctga acggtagcgc
    14341 gctcaacaga atgacggtca gcccatcggt taatttcatt ttcgcccttg cgcagaaggg
    14401 tgctgatttt tttatattca tttgatgaat ccatacaggt ggaaaaacaa gcaggactgc
    14461 catttatacg cgcgcataac aacgccgcaa gctgattatc cggctgtgta ttgacccgtg
    14521 tgctgtgaaa gtgaaatagg gtggagagag agtgtttcgg ccagttggca aagttctgca
    14581 gttgaacttt ttgtgtgctc ttcaggtagt ttttccacct catagctgct tcgccaacgt
    14641 attgattcat cagcaaacag tgacgcgtcg gcgtagtttt gtatcgccag cccgggtaac
    14701 cagcgtaccg gatgcccgag ataagcctga aatgtcgcaa ttgtccgcgc ctgaaagcca
    14761 ataaaaccaa tgatttggtt aatgatcaca cagtcccaga cagtaagacc gacgtcattc
    14821 agatgctggc gcgatcggtt gtcgataata gaaggcgagc tagccagttg gcgggcatat
    14881 tgcgtgattt gtgccaaccg atgattactt tccctggagg aatcaggtcc ggggagcggc
    14941 gtaagtcgcg ccgaatagta gttacataac cgctggacgc cgcaaacctg agcaaccgtc
    15001 aaggccgtcg acaagcgttc ataagcggtg aaggtttgtg aacgggtcac ggtaagctgg
    15061 tcagggaaca actccactgc aagaacgcgc gcagggacca gccatgaaag gaagggggcc
    15121 agcgtctctt catcaaggat cacgtcgagc agaaagggat cgctgacctt tgcggcttcc
    15181 gggaccagag gcagaacgtc atactccgta gtactggatt gcgtttcatg ataccagtgg
    15241 cttttgccgg tgatgtggcg ttgttccatg ggcgctcctt ggtcgtaaag gaaatcgtta
    15301 tcctgacgca aggcgggaag gggagaaaga cggatcggcg ataacaaata tcagaaaggt
    15361 ataacagaga taacgggcgg cagaacgccg cccatcttta ccaacagaac gattatttca
    15421 gttcgagttc gttcattgca gcgatgctga aaccgccgtc aacgtggact acttcaccgg
    15481 agataccggc agagagatcg gagcacagga atgccgcaga gttacccaca tcttcaatag
    15541 taacggtacg gcgaatcggg gtaacggctt cgcaatgagc cagcattttg cggaagtctt
    15601 tgataccgga ggccgccaga gtacggatcg gaccagcaga gatggcgtta acacgcacac
    15661 cttccggacc catcgcgttc gccatatagc gcacgttcgc ttccagagac gcttttgcca
    15721 gacccataac gttgtagttc gggatagcgc gctcagcgcc aaggtaggaa agggtcagca
    15781 gggcagaacc cggattcagc atggagcggc aagcttttgc cattgcaacg aagctgtagg
    15841 agctgatgtc gtgggcaatt ttgaagcctt cacgggtaac ggcgttaaca tagtcaccat
    15901 ccagctgatc gccaggtgca aaaccaatag agtgtacgaa accgtcaaat ttcggccaaa
    15961 ctttccccag ttcagcgaac atggtgtcga tgctggcatc ttctgcaaca tcgcactgca
    16021 gaacgatgtc agaacccaat tgagcggcaa attcttctac gcggcctttc agtttgtcgt
    16081 tctggtaggt gaatgccagt tcagctcctt cgcggtgcat cgcctgagcg ataccgtagg
    16141 cgatggacag tttgctggca acaccggtta ccagaatgcg cttaccggaa agaaaaccca
    16201 tagctttaat ccttattgtt gatgcttgtt gtgcctgaaa atcaggcgat tcgtcgtttt
    16261 agtaaacagt acgaacagat aaacggttat tataatcaac ctggctgtga gtagctatag
    16321 ttgccaggtc cgaccggagc aggctgcggc agggggggcg cttttcccct taccctaacc
    16381 ctctccccag aggggcgagg ggaccatatt gtgcaaatat tgttacccca gcaacaaaca
    16441 ggctcataca gcccctaacc cttttatggc gatggctggg atggttcaga ccttgccgaa
    16501 tattctccag cactgtttcc atgttttcac cacaacagct attcggctcg gtcggccccc
    16561 tcgccctttc agggtgaggg tgaccggcgg ttcagttcct gcagaataat ctccagcacc
    16621 gcctcctcat tacaatcaat ctcgttattc cagaaacgca gcacggtcca gccctgcgac
    16681 tcaagccagc tagtgcgcct ggtatcatag gcaactgcca aatcatgctg cccaccatcc
    16741 agctcaacga ctacgcgcgc cgagcagcaa gcaaaatcga gaatgtagct ccccaccgga
    16801 tgttgacggc gaaatttgaa atcaccaaaa cgtcggctgc gaagatatcg ccagagcttt
    16861 cgttcctgca aagtgagatt gcgtcttaaa tcacgcgcat ttgattttat tttatccatc
    16921 acctcattct gacaagattt aatctcttgt cagaatgagg tgaataatct ggaaggagga
    16981 ttcagaaaat tagcgatctt tacgccacgc atccgctgtc aatgcttcgc caaagtgccc
    17041 ggcaatcagc cgtttggtga gttcatgcag cggcgatgcc agcacatccg cggtgctgcc
    17101 tcgctcgaca acctcgccct gatgcatcac cagcacctgg tcgctaatgt gcttcatcat
    17161 tccgatatgc tgggtaacat aaatatacga aatgccctgt ttttcctgta actccagcat
    17221 cagattaatc aactgcgaac gcatcgacat atccagtgaa gcgagggctt catcggcaat
    17281 aatgactttt gggcgcaata tcagcgcgcg cgccagaccc agacgctgtt tttgtccggg
    17341 tgccaacata tgcggatagt aactgacgtg atccggtagc agcccaacca tacgcatcgt
    17401 ttcaataatc tgtttgcgac gctgttccgg ttccaggtcg gtgttcaggc gcagtggaaa
    17461 atccagaatt tgcgagatac gttgacgggg attcaacgag gtcgaaggat cctgaaaaat
    17521 catgcgaata cgctgactac ggaaggaata atcgccaaaa tgcagcggat gatcgtcaat
    17581 caataactcg ccgctggtag gttctatcat tcccgccagc atttttgcca gtgtggattt
    17641 acccgaacca ttctcgccaa taatcgccag tgtctggcct tcacgtagcg taaagctcaa
    17701 gggttttacc gcttctacgg tctgacgacg aaaccaaccg gtccggtagc ggaacgtttt
    17761 acttaaatta cgcacttcaa gcagcgtttc gatcatctca ctctttctcc atgttcagcg
    17821 ggaaatgaca ggcatagaga tgatttttcg cccccatcaa acgtggcgtc acaatgcatt
    17881 ctcgttgtgc atacgggcaa cgtggcccca gacgacaacc aatcggtaac tgttccagca
    17941 atgggatagc gccgggtaga gtattgaggc gacttttatg cggcatcgcg ctgccgaagt
    18001 ctggtatcgc gcggatcagc gcctgggtat aaggatgatg tggcatcgtc accaactcct
    18061 tactcggcgc ggtttccact gtttgaccgc agtaaagcac gttaatttta tccgcccatt
    18121 ggctaagcat ttgtaagtca tggctgataa gcaaaatagt ggtattgctg ttttggttga
    18181 gacgcgtcag caggcgaaag atttgcgcct gggttgttgg ctccattgag tttgtcggtt
    18241 cgtcagcaat cagcagacgc ggttgattcg ccagtgcaat ggctatcatc actttctgac
    18301 attcaccttc ggtcaactca tagggaaaac tgcgcatcgc atctttgtga tctttaatcc
    18361 ccacgcggtg cagcagttca atggcacggc gtttgcgcca gccaaaacgc tgccaccaac
    18421 ggcctttata ggtccaggct gggatgtttt gcatcaactg gcgacccaca cgttctgaag
    18481 ggtcaagaca cgactgcggt tcctggaaaa tcatcgacac gttatggcca accagtttgc
    18541 gccgttcgcg tgcggagaga cgcagcaaat cgatatcatc aaaacgcata cggtcagcag
    18601 taacacgcca gttatcttta ttcaccccac aaatcgcttt cgcaatcaaa ctcttgccgg
    18661 aaccggattc accaacaaga ccgcggattt caccttcggt taaggtcata cttacccggt
    18721 cgacggcttt aacccactca tcaccggttt taaattcaat ggtcaggtta cgaatatcga
    18781 gtaatggcat tattccaccc ccgcaataat cgcacgacga acgccatcgc caaggaggtt
    18841 aaccaacaac acgctaatca taattgccgc acctggcagc atgacagtcc acggggcgac
    18901 ataaatcagt tccagcgcat caccgagcat cgctccccat tcaggcgagg ggagttgtgc
    18961 gccgagatcg agaaagccca gcgcggcgat atcgagaatt gccatcgaca gtgcgcgggt
    19021 gatctcggtg accagcccgg cggtgatgtt tggcatcaca gcaaaccaga gaatattcag
    19081 cgttgatgcg ccatccagac gggcggcgat aacgtactct ttttccagtt cgtcatgcac
    19141 catgctgtaa atcgaacgta ccatacgcgg cagcaatgcc agccagacag caaacatagc
    19201 gtgggacaaa ctcggtccgg caaacgctac aacgataatt gccaacagca gcgaagggat
    19261 cgccagtaag gtatccagaa tatggttaag caccgctgag cggaggccgt gtgtcgcccc
    19321 ggcgaaggtg cccagtacca ggccacagat cgtcgcggca agcgtcacca caaatgcgcc
    19381 acccacggtt ggcgcagctc cgctcagtaa acggcttaaa acatcacgtc cgaggtcgtc
    19441 agtccccagg aagaaagaaa ctttgccata gcgcgaccat gatggcggca ataattgata
    19501 accgagaaat tgctggtcga tgccgtaagg cgcaaaccag ccgccaaaaa tacacagtac
    19561 agccagtccc gcgcagccgt acaggccgac catcgcagag gcgtcactat aaaatttgcg
    19621 ccaggcggta cgcagcgtgc ccggcgggcg cttttcgctg tatacgctat cgtaaggcat
    19681 accattcctt atgtttcaga gggttagcca tggcacccaa aatatcagaa atcacgttaa
    19741 caataatgac cagtgagcca cacaccatca ctccggcgga aatggctgcg taatcctgct
    19801 ggcggatggc gttaattaac cagcgcccta aacccggcca gctaaaaacc atttcggtga
    19861 tcatcgccag cgtcagcatg gtggaaaact gtaagcccag gcgaggaata accggcggta
    19921 acgcgttatg cagaacgtgg cgacgcaaaa tagtaaagcg cgacaaaccg cgggtcgccg
    19981 ccgcttttac atagttctgg tcatacactt caatagtgct gatacgcatc aggcggatca
    20041 cttcagttgt tggcgcaacc gacagggtga tcacgggcaa tatcatatgg cggattgcgc
    20101 tcatgatcat ttcatcccgc catggtgagt ccgaaagcca ggcatcaatc aacgcaaaac
    20161 cggtaatcgg tttcacttcg tagagcagat cgaaacgccc tgaaacgggc aaccaaccta
    20221 gcgtgagtga acaaaacagt gtcaacagaa gcgccagcca gaacaccggg attgaaaagc
    20281 ccagtaaggc gatggcgttg atcaagttat cctgccactt atggcgcgta atcccggcaa
    20341 tcatccccac ggggatgcca acaatcagcg caaaaccgaa ggcgaggatg cacagttcta
    20401 tcgtcgccgg gaagacctct tttaactgtt cggcaattgg ttgaccgtta atgctggaaa
    20461 caccaaaatc ccagtggatc aggccattaa accagaacac ccaggcattc cacagtgacg
    20521 cgccttgtaa cggcgcgtgt ggagtgaaat agctcaagct gaagccaacg aaggtcagca
    20581 ggaacaaggt gacaatcaat aacaaaatgc ggcgtaaggt gaagataatc atggtttttt
    20641 cacctcatcc tgtttctcgc gatacacccc agcaaaggag gcgttaccaa acgggctaag
    20701 taccagacct ttgatatcgt accgataggc ctgcaaacgc aatgatgacg ccagcggcag
    20761 aataggtaat tcctgcgcca gaatactctg cgcttcgtca taggcttcaa tacgcgctgc
    20821 cagctgctgc gaggagagcg ccttacgcaa cacgctgtcg aatttcggat cgcaccagtg
    20881 ggcgaggttg gtctgtgaat gaattgccgc gcagctcagt aacggacgga agaaactgtc
    20941 cgggtcgtta ctgtccgtcg cccaaccgga taacgtcaga tcatggctca tatccatcaa
    21001 ccgcgcctcc tgaaagcgac cttctaccgg cacaatcacc acttttacgc caacctgcgc
    21061 catatccgcc tgaatcagtt cggcagtttt cagtggactg gggttccacg cctgcgaacg
    21121 tgtgggcacc cacagtttca gcgttaagtt ttccagcccc aacgccttca actgttcgcg
    21181 cgatttcgcc ggattgtatt cagtaatttt agcctcgttg tcataggccc acgaggcgcg
    21241 cggtaaaata gaggccgccg tttcagccgt accataatag atggattgca tcaggcgctg
    21301 gttattaatc gccagtgcca gtgcatggcg gacagcggga ttatttagcg gcggtttggc
    21361 ggtgttaaat gccagatagg cgacgttcat cccaggacgc agcgttaaac gcaagcgcgg
    21421 gtcgtcacgc aaaatggata gctggctggc agcaggccag gccagaacgt cgcattcccc
    21481 ggtcaggagt ttcgacagac gtccggtgcc gccggagcct aaatccacca ctacctgcgg
    21541 cattaacggt ttaccgcgcc agaagtcatc atgacgttgt aggcgaataa attgcccggc
    21601 gcggtattcc gacaactgat acggaccggt gccgaccggt tgacggtcga gttgctcctg
    21661 gcgatcttct ttctctaact tccgggcata ttctgccgac atgaccgaag cataatgggt
    21721 tgcgaggtgc cacaaaaaag aagcatccgg ctgggcgagt cggaactcaa cggtatgatt
    21781 atccagtttg cggacgcttt tcacgttatc ggcaaattgc aggctgtcga agtaggggaa
    21841 gttgctgccg ttgacgttat gccacgggtt gttgcggtca aaaattcgct ggaaagtaaa
    21901 caccacatcg tcggcattca ttttacgagt gggagtaaac cagtcggttt tttgaaacgg
    21961 aacatcgcga cgcaggtgga agcgataggt cgcgccgttg tcgagtactt cccagctttc
    22021 ggcaagttcc ggcatcaggc gataggtata gggatcgaca tccagcagtc gatcataaaa
    22081 ctgggcggca agggtatcga caattaaccc actgctcgct ttggatgggt taaaggtgtt
    22141 gacttgcccg ctgacgcaat agacaaaacc gctgtcgcgg atatcagcat gcggggggga
    22201 ttcaggcgcg gcgattgcct gaccactcac aagtccagca atcaccaaaa gagacgataa
    22261 tacctggcgc ataatattaa gggattttat gtaaagaggc tatcttacta atatttaatg
    22321 acatttgcca ttaccgtttg tgttcagggg tcgtaatgag ggccgtgtgg ttggtcgttg
    22381 aacaggtggc gtcgccatct gttcaacatt cgtacccgtg atttctctat aactataact
    22441 cacagaacaa cttagcgagg agcagagcgg tacgatcgtg agaaacccat taaagcacgc
    22501 tgcagtgcgt gtcattgtca gccggatgcg gcgtgaacgc tttatccaga caacgatact
    22561 gaccgatcgt ctgcaattaa ataattactc attaccccat tgattcaaaa actctgcgat
    22621 ctcatcaatg cgtacgggat taatccccgc ttcagcagcc atttcatgtt gggcttcttc
    22681 gctgatctct tcattgttca tcaaacgggt gagcagtaac tggaagtagt gggccagcgg
    22741 tgtgcgttca gcctctgcga caggctctgc gcattcggtc gcgtactcat cggcaatatc
    22801 aaaatatttt agcgggatat cgtggttcat tatttgcccc tggggttaat gcgctaagca
    22861 taagggcgga tgatagcact cgtgatccgg gtcatacatc tttttatagt taatggcgcg
    22921 gcgcgttacc ctcaggcagg ataatgcgcc gcgcatccgg ctattacgtt tcgctcaccg
    22981 gcgttctgtc accttcgtac agcggcactt tgcgatagcg gcggatcaga taccacaaat
    23041 atgcgccgcc taacgaagcc cataccagac cgagtgtcag tgacgttgac tctaggttaa
    23101 cccacagcac acccaccgtc agcgcgccaa ccagcggcat cagcaaatag tggaagtgat
    23161 ccttccagct tttattcatt cctttacgcc gccagaaatg attaaacacc gacagattca
    23221 cgaaggtaaa cgccaccaga gcaccgaagt taatcagtgc tgtcgcggtg actaaatcaa
    23281 agaacagcgc cgacagcgcg acaatcccga ccataatgac gttgagtgcc ggagtccgcc
    23341 attttgggtg cacatagcca aacacgcgct ccggaaacac attgtcacgc cccatcacat
    23401 acagcagacg cgacacgctg gcatgtgaag ccaggccaga cgctaatgtg ttcacaaacg
    23461 tggtgcagag gaaaatcgac tgaaagagct tgccgccgac gtacagcgca atttcaggca
    23521 gtgcggcgtc cgggtcttta aagcggctga tatcgggaaa gaacagctgc ataaagaacg
    23581 acgccgcgat aaagataacg ccgccatata ccgccgtcag gaagatggct ttcgggatca
    23641 cgcgtgcggc gtccggcgtc tcttccgaaa gcgtggtcac cgcatcgaaa ccgaggaacg
    23701 agaaacagac aatcgtcgca ccggtaataa tcgggatcag gtgcgcgttc tcgctgataa
    23761 acggctgaag tgaccagacg gtgccaacgc cttctccttt atgcagtccc tgaaccacca
    23821 ggaagataaa caccaccatg atggagattt gcaccagtac aaacagggta ttgaagttag
    23881 cgaccaggtt gacgctcttc agattcgcgg cggttaaaat ggcgacgaag gttaccaccc
    23941 acacccacgg cggcacttcc gggaagaggg cggagagata gattttcgcc aacaaaacgt
    24001 tgatcattgg caaaaagaga taatccagca gtgatgacca gccgactata aatccgacgt
    24061 gcgggttaat cgacttttgc gcgtaggtat aggccgaacc ggcctccgga aactggcgaa
    24121 ccagtttgcc gtagctgata gcggtaaaca gcacgcccgc cagcgccagc aaataggacg
    24181 ccggaacgtg accgtcgcta atgccggaga caataccaaa agtatcaaat acggtcatcg
    24241 gcgtgagata ggccagaccc atcattacca cctgccacaa tttcagtgat tttcgcagac
    24301 gggttttgcc gggttgcgca gcaatattca gtggtgaatt aatagccata attgctgcct
    24361 cccctgcgta ataaggtttg cggtcgtgtc gacctgaaat caaacctgtt tagcaaggga
    24421 agcaaagggg gcggagtcca gtcagtagca ggaggggcgc atacgcagcc tccctcgcca
    24481 tgcggggatt taatcatcgg catcatctca tttcctcgtc acagtcttaa tcgttaacgg
    24541 aagcccgttg ccgcctgcgc tccataacgc gacatccggg aaatgtctgg attaccagca
    24601 aatgtcaggc ctggctccgc tcaggccgat gaaacaaccc cgcaagggga attacgcgtt
    24661 tttcaacatc cactcaattt ctgtttctgt gatgaggcgc tcaaactgca acagctcatc
    24721 atttttacag gcgtgataca catggcaaaa ccgttcgcct aaatagcggc gcaggtgatc
    24781 attctcgata aactcaccca gggcatcgct ctggcgaatc gggaagggta agccttcctg
    24841 ttccagcccg ttgccttcga cttcttcctg caacggcagc tcgttatcaa ggccatgcaa
    24901 aataccggca aaaatcgctg ccatcaccag atatgggttg gcatcggcac ccgccacgtg
    24961 atattccacg cggtgattat gacggtcgcc gcacggaata cgcagggcga cggtgcggtt
    25021 gttatggccc cacgacgcct gcgtcggcac atacattccc ggctggaagc ggcgatacga
    25081 gttcacgttt ggtgccagca acgccatcga cgacggcatc aggtcaatca tcccggcgag
    25141 catctttttc agcagcggcg aatcttcgcc ttctgtgtca gaaagcacat tctcgccacg
    25201 attattttgc atactgatat ggatatgcat cccgctgccc gcgtgctctt catacggctt
    25261 cgccataaaa gtggcgtgca tcttatgctt ttctgccatc agacgcacca ggcgttttag
    25321 tgccagcgca tcatcgcagg cttccagcac gttatcagta tggtaaaggt tgatttcaaa
    25381 ctgacccggc gaggcctcag cgaccgcgcc atctgccgga atcagctgca actgcgccag
    25441 ttcatcaata tcattgagca cgtcggcgaa gtggttgagg ttatcaacgg agtaaacctg
    25501 gctttgtgta ttgcggtcat cggtgccagg cgcgcagggc ggttgcagat ccccttcagc
    25561 gtcgcgctgg cgatccagta aatagaactc cagctctacc gctacaaccg ggaacaatcc
    25621 gcgctggcgc agctgctgcc acaggcggtt aagaacgttc cgcggctcaa cgtcaaaggg
    25681 agcgccatct tcatcgacca tggtcaggag catctgaccg ataaactctg gatcggcggc
    25741 agaaggagtt aaggaaccga gaacaggaac acaggtacga tccggctcgc ccatttcctg
    25801 acccagacct gcttcttcta ctacgttgcc cagaatatcc attgcaaata ccgaggccgg
    25861 gaaataatac cct
//
LOCUS       DADKXM010000046        25406 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.046, whole genome shotgun sequence.
ACCESSION   DADKXM010000046 DADKXM010000000
VERSION     DADKXM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.046"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..497
                     /gene="tnpB"
                     /locus_tag="JQR05_001876"
     CDS             <1..497
                     /gene="tnpB"
                     /locus_tag="JQR05_001876"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY5324058.1"
                     /translation="GLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKF
                     SNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTV
                     SQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPALRVLWSYSERKP
                     PVTK"
     gene            430..657
                     /locus_tag="JQR05_001877"
     CDS             430..657
                     /locus_tag="JQR05_001877"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5324059.1"
                     /translation="MLFRQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(701..5317)
                     /locus_tag="JQR05_001878"
                     /pseudo
     CDS             complement(701..5317)
                     /locus_tag="JQR05_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5410..6057)
                     /gene="rnt"
                     /locus_tag="JQR05_001879"
     CDS             complement(5410..6057)
                     /gene="rnt"
                     /locus_tag="JQR05_001879"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY5324060.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(6160..6567)
                     /gene="gloA"
                     /locus_tag="JQR05_001880"
     CDS             complement(6160..6567)
                     /gene="gloA"
                     /locus_tag="JQR05_001880"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY5324061.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6648..7733)
                     /gene="nemA"
                     /locus_tag="JQR05_001881"
     CDS             complement(6648..7733)
                     /gene="nemA"
                     /locus_tag="JQR05_001881"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY5324062.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISDSAQTKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTG
                     RISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDF
                     RQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAG
                     IEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGE
                     PYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQ
                     LKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7770..8369)
                     /locus_tag="JQR05_001882"
     CDS             complement(7770..8369)
                     /locus_tag="JQR05_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5324063.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8472..8711
                     /locus_tag="JQR05_001883"
     CDS             8472..8711
                     /locus_tag="JQR05_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY5324064.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8760..9656
                     /locus_tag="JQR05_001884"
     CDS             8760..9656
                     /locus_tag="JQR05_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY5324065.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9737..10258
                     /gene="sodC"
                     /locus_tag="JQR05_001885"
     CDS             9737..10258
                     /gene="sodC"
                     /locus_tag="JQR05_001885"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY5324066.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10259..12271)
                     /locus_tag="JQR05_001886"
                     /pseudo
     CDS             complement(10259..12271)
                     /locus_tag="JQR05_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(12271..13128)
                     /locus_tag="JQR05_001887"
     CDS             complement(12271..13128)
                     /locus_tag="JQR05_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5324067.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNVAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(13131..13367)
                     /locus_tag="JQR05_001888"
     CDS             complement(13131..13367)
                     /locus_tag="JQR05_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY5324068.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13568..14002
                     /gene="slyA"
                     /locus_tag="JQR05_001889"
     CDS             13568..14002
                     /gene="slyA"
                     /locus_tag="JQR05_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY5324069.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLDHNIIELQAKG"
     gene            complement(14049..14516)
                     /gene="slyB"
                     /locus_tag="JQR05_001890"
     CDS             complement(14049..14516)
                     /gene="slyB"
                     /locus_tag="JQR05_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY5324070.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14790..15899
                     /gene="anmK"
                     /locus_tag="JQR05_001891"
     CDS             14790..15899
                     /gene="anmK"
                     /locus_tag="JQR05_001891"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY5324071.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15997..16326
                     /gene="mliC"
                     /locus_tag="JQR05_001892"
     CDS             15997..16326
                     /gene="mliC"
                     /locus_tag="JQR05_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY5324072.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16385..17041
                     /gene="pdxH"
                     /locus_tag="JQR05_001893"
     CDS             16385..17041
                     /gene="pdxH"
                     /locus_tag="JQR05_001893"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY5324073.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            17170..18444
                     /gene="tyrS"
                     /locus_tag="JQR05_001894"
     CDS             17170..18444
                     /gene="tyrS"
                     /locus_tag="JQR05_001894"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY5324074.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18506..19366
                     /gene="pdxY"
                     /locus_tag="JQR05_001895"
     CDS             18506..19366
                     /gene="pdxY"
                     /locus_tag="JQR05_001895"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY5324075.1"
                     /translation="MKNILAIHSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(19410..20015)
                     /gene="gstA"
                     /locus_tag="JQR05_001896"
     CDS             complement(19410..20015)
                     /gene="gstA"
                     /locus_tag="JQR05_001896"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY5324076.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(20121..21623)
                     /gene="dtpA"
                     /locus_tag="JQR05_001897"
     CDS             complement(20121..21623)
                     /gene="dtpA"
                     /locus_tag="JQR05_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY5324077.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(22234..22869)
                     /gene="nth"
                     /locus_tag="JQR05_001898"
     CDS             complement(22234..22869)
                     /gene="nth"
                     /locus_tag="JQR05_001898"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY5324078.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22869..23564)
                     /locus_tag="JQR05_001899"
     CDS             complement(22869..23564)
                     /locus_tag="JQR05_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY5324079.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23568..24188)
                     /gene="rsxG"
                     /locus_tag="JQR05_001900"
     CDS             complement(23568..24188)
                     /gene="rsxG"
                     /locus_tag="JQR05_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY5324080.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(24192..25250)
                     /gene="rsxD"
                     /locus_tag="JQR05_001901"
     CDS             complement(24192..25250)
                     /gene="rsxD"
                     /locus_tag="JQR05_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY5324081.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         6308 a         6565 c         6630 g         5903 t
ORIGIN      
        1 tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca gtctttgagc
       61 ctgtaaacag ttttcaaaaa aaccagaaga cgctggcgag acttcagcga cagttaagcc
      121 ggaaggtcaa attcagcaac aactggcaga agcagaaacg caaaatacag cgactgcatt
      181 cctgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacgacc gtcagcaaaa
      241 accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag tcagcagcgg
      301 gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac cgttcgatac
      361 tggatcaggg ctggtatgaa atgcgccgcc agcttgagta caagcagctc tggcgtggcg
      421 gtcaggtgct tgctgttccg ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaac
      481 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
      541 aatggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      601 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcttgaacg
      661 tagcagggaa ccacgtcctt cagggcgtgg aggatgtcaa ctatccccaa tccagccctt
      721 gtggcgaact tgaaaatccc gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg
      781 gaagatcatt cacttctgtt agcgtaaagc gcagccttgg ttcgcgacgc agtgcggtgg
      841 tcagcgcctg gaaaatctct ggggcgagta attcctcctt ttcctgccag accagcattt
      901 ttttgccgcc ttgcgccaga tagagtaaca atttgccgcc agaaacgacc accagcgcac
      961 ccgcccgacg cgttggtacc agcgttgccg gatgtgcagg ccagggcaga agatttcccc
     1021 acacattagc cggatcgttg gcggagagcg ccactggtgt atagtggcgt gtttgcgtgg
     1081 cttgtgtcgc cagatcgcgc aatcggtcaa tagtcagacg ttcagcgaat tgcgcgccac
     1141 ccagaccttc tacaaaacga cctcgcataa ttcgcccgga gtcttccata cttcgacaaa
     1201 gtgtttgcat cgatggaaac ccgccaggga tattttcggc tatcaccgcc tgacgactga
     1261 tgatgccgta gcggtcgagc atattttccg ccagcgctag catcctttcg gtatcgttta
     1321 gtggctccac ctgcaataac gaccagcgtc cagccagatt tggtgtgttg taagataccc
     1381 gcggcgagac gggttgtgca tagacaggac gcccgcgacg agcccggtga ctgcggcgag
     1441 ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg taacggtgcc caaatatcgc
     1501 tggtaatgac gccttgccag accagcgccc ataacgcctc ttgcagtgcc gaggaatcaa
     1561 ccgattcgcc gattttatcg cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc
     1621 cgtcagccag aacagcgatt atcgcttgct gcagcgccga acgattcgcc tgatccgctt
     1681 cggcgggagt gaacgattct gcggcatatt tctgtagatg cagtgccacc aggccgtcat
     1741 cttcacccag ctttttttgt cccgaccaga taaccgctcc ggttgccagt aattcatcga
     1801 gcatttctgg cgaatagtcg cgtacgcggg caggcaggat ctggctttcc cagagtgagg
     1861 cgggtaaacc gactccggca agctgttcga tcacccgcat cacgccatct acgccctcat
     1921 aaacgcctgg ctatgttgag gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc
     1981 cctgacgttc caacagcaat cgcgcatagg tcgtggctgc aacgggacgc gtcgcttctc
     2041 tggcggcttg cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc acccagatgt
     2101 cttgttgcag attcatcacc agaccctgtt cacgcagttg ctgaagctgc tcttcgacaa
     2161 tggcaatacc gagactaaac tcatgagcca gttgttcagc cgtgaccaga gcatgagccc
     2221 ggagatagcg cagaaagagg tcgcgaagcg ggtaactgac tctatgtaaa taaatctctg
     2281 gcaatgactc tggtagtcgt acgccgaggg catcacgcag cctggcggca tcatccatac
     2341 aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat tcgttttact gcaagaagat
     2401 tttccagata cgacgcaacc tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa
     2461 cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc
     2521 tgccaggagc cagtcgttgc aactcttctt ccacctggcg aatgacctgc gggtcgagta
     2581 attcccccgg atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta
     2641 cggatgcccg gcgctctgcc agcggggcgt cgctctggta cataaattcc gcgacatagc
     2701 cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt cgttacatcg gatatttgaa
     2761 tttcgccacc gttcagacga cgcatcaaac gttccagtgc gggaagatca taaacatctt
     2821 gcagacattc gcgtagggtt tcgagaatga ccggaaaatc cggatatccc tgagcgattt
     2881 ccagcaactg actggcgcgc agccgttgtt gccaaagcgg ggtgcgatgg cccggcgtgc
     2941 ggcccggcat taataatgcc cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg
     3001 agctgcctac cgcctcgcgg acaatttgca gcaacttttc tggttcaaac aaaaaaatcg
     3061 cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc aacaatgccg tcatcactgg
     3121 cgaccacaga cgcgtcagcg ccccataggg catgtattcg cccggcaatc gccagcgccc
     3181 agggttcatg cacccgtctt ccatagggag agtgcaaaat aatacgccag tcgccaattt
     3241 catcacggca ccgttcgagg accagatggc gactccccgg aacgatgccc gtcgcgttgc
     3301 gttgctcgtc aatcaacccc tgaatattgg cgttcgtatt ttcttctgcc agccacgggg
     3361 gaatagtccc ggaaaagaac gcgccatccg ccagtaaatg aagaaaatcg ccgatcatct
     3421 cgcctaattc agccggacgt ccgttacctt caccacgcca gaaggggagc cgggcagaac
     3481 gaccctgagc aggagtcaca atcacctgat cgcgggtgat ttgctggatc cgccatgagg
     3541 tagcgccgag agtgataatg tcgttcaccc gcgactcata taccatctcc tcatccagtt
     3601 cacccacccg ccgcgaaccg gccttttctt caccttcggg taataacacg ctatacatgc
     3661 cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa ttgagcgcca ggtcgggcgg
     3721 tcaatatccc ggtctccctg ttccagacca gtttggggcg aaaagcagaa aaatcgccag
     3781 agggatagcg cccggaaagc atatccagcg tggcgtcaaa aacacgtctt ggcagatctt
     3841 tccacggtgc ggcacggcgt acgcgggagt accattcgtc tacctgtaat gcatccatcg
     3901 ccgcggcggc aacggtttgc tgcgcaagga cgtcgagagg attatgcggt ggtgtcaggt
     3961 tttccagcct gcctgcgaac atacactcta caatgactgc ggaatcgact aaatcacgcc
     4021 gggtacgggg gaaaaacagc cctttagata caccgccaac ctgatgtccg gcgcgaccaa
     4081 tgcgttgtaa cccactggca acagaaagcg gcgttgccac ctgaatcacc agatccaccg
     4141 cgcccatatc aatccccagt tcaagactgg aggttgcgac cacgcagcgt aattcacccg
     4201 atttcagcgc ctgttcggtg attgctcgtt gttctttaga gacggagccg tggtgtgagc
     4261 gggcaataaa aacgtcgcta ctttgtacac ggttagaggt tgcgccggag gtcgactcga
     4321 aatgggccgc atcaacggcg atagacgggg aacgctgtaa gcgtgcggcg taaagctcat
     4381 ttaatcgtgc cgtcagtttt tccgccagcc cgcgtgaatt agtaaagaca atggtcgagc
     4441 gatggcgcaa cacttcatca aggatacccg tttcaatata tggccagatg gagccttccc
     4501 ggccggcatg gctgtcttcg ccggtgccgc tggcgaccga tgagacatca tccatattgg
     4561 cgaccggtac gacaattcgt atctgcggat ggcgcattgc tggcgggttg actaccgtaa
     4621 ccgggcgatc gccaccaaga aatgctgcca catcgctggc tgagcgcaca gtggcagaaa
     4681 ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga cttaacgcca
     4741 gatgcgcacc acgtttactg cccgctaccg cgtggacttc atcaataatt accgtttcga
     4801 cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg
     4861 tgatcagaat atccggcgga ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc
     4921 cagtacggat ccctacgcga agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg
     4981 caataccctt caacgggatc tgcaagttgc gctgaacgtc ggtgcccagg gcttttatcg
     5041 gtgaaatata gaggatgcgt gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc
     5101 cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc cagcgttttc ccggagccag
     5161 tcggtgcgat caccagcgca tgttcgcctc gcgccgccac atgccaggtt tgcgactgga
     5221 cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt
     5281 ccggcaggag cgatgaaggg tctggattat ctgccattaa cgctattccc cgtgaatatc
     5341 atgcttgatg cgtggaatag cctcactata gatgcctgaa tcattgcatg tcatcaggca
     5401 tcgactcgat tacacctctt cggcggcagg tagcggccag cctcccagac gtttccagcg
     5461 gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc
     5521 ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc tttgacaata cggtttgtcc
     5581 gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga acgggttacg
     5641 tttcagcgag gcgcgttctg cggcggccat cataaagctg tgatcaaaat tggcgttgtg
     5701 cgccaccata atggcgcggt tacagccgct cgctttaata cctttacgta caactttaaa
     5761 aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat
     5821 gccgttgaag gcgagggctt ctggttgcaa atttgcgccg acaaatggtt cgacgtggaa
     5881 atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc attttcaggg tgatggcggc
     5941 aatctcaagc agcgcatcgg ttttggcgtt aaatccggct gtttcaacat cgatcacaac
     6001 aggataaaaa ccacgaaaac ggtcgcacag accggtaagt tgagcgttat cggacatctg
     6061 attctcttaa tacggataaa ttgcagcgcg cattatgaca aatattactt gtcgatgcag
     6121 taaagatgcg ggcgcgatga gttcacgccc ggcaggagat tagttgccca gaccgcgacc
     6181 ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc ggatcttcca caaacgcgat
     6241 aaccgtagta ccgcctttta ccggacccgc ttcacgggtc acgttacccc cgttttgacg
     6301 gattttttcg cacgcttcag cggcgttatc tacgctaagc gcgatgtgac cataagcagt
     6361 gccgagttcg tatttatcca cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt
     6421 ttccgggccg tagccaacaa acgccagtga gtatttgtat tccgggtttt cgctggtacg
     6481 cagcagtttc atgcccagca ctttggtata aaaatcgatg gagcgttgca aatcgccaac
     6541 gcgcagcatg gtatgaagaa gacgcatctt tttatcctca aaatggctaa cagattcaaa
     6601 atgttgtttt agtatagcgg cggctttacg ccgctcgcaa tgttggatta caacgtcggg
     6661 taatcggtat agccttccgc gccgccaccg tagaaacttt cggcacgctg tgggttaagc
     6721 tcagctttga gctgcaagcg ggcgaccaga tccgggttcg caatccagtc acgaccaaat
     6781 gccaccgcat caattaaccc tttgccgatc agcgtttcag ctttttctac tgtgtatgca
     6841 cctgcgccga taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta
     6901 tacggttcac cccccgccca atctggttct gacatatgca gataagcaat gccgcgttta
     6961 cccagttgtt caatcagata cagtgcatcg gcttcttcat tcgggccgtt atctgtgttc
     7021 tggaaagtac cgattggcga aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc
     7081 ccggcatcga ctacttccag taccaaacgt gcgcgatttt ccacgctgcc gccgtactga
     7141 tcggtacgat ggtttgaaga aggggaaagg aactgatgca gcaaataacc gtgagcagag
     7201 tggagctcta ccagatcaaa accggcttca cgcgcgttag caatggcctg acggaaatca
     7261 ttgacgatac ccggaatctc ttccagttca agcgcacgcg gcatggatgt ttcaacgcgg
     7321 atcgcctgac cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc
     7381 gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac
     7441 agctgcaagg ccatatgacc attttcagca tgaacgccag cggtgatttt tttccatgcg
     7501 gcaatttgct ccggactatg gatgccaggc gcacctgcat atccttttgt ctgggcagaa
     7561 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
     7621 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
     7681 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
     7741 cccggtcgtc tggtgataaa aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
     7801 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
     7861 gcgccaagcc atagtgcgta aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt
     7921 aaacaatggt tctcacggcc attttccaga gcctgcgaga gcagggcgat cacgccgcga
     7981 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
     8041 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
     8101 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
     8161 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
     8221 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
     8281 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccaa gagatgttcg
     8341 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
     8401 tgcaagcaaa gttgctagtt tggacattaa cagcttcaat aatggtaata atcttatctg
     8461 aggagggcga ggtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
     8521 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgg gatgagcgtt
     8581 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
     8641 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
     8701 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
     8761 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct
     8821 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
     8881 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
     8941 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
     9001 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
     9061 ctgaccgcaa tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
     9121 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
     9181 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
     9241 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
     9301 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
     9361 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
     9421 attatttcca gccgctgcgt gatgaactgg ctgtggtggc ggaggagtta aacgcgggct
     9481 cgattgaaca ggtggtttac gcctgggtat tacgtttacc gtcgcagccg ctgccaatta
     9541 tcggctcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
     9601 cccgtcaaca atggtttcgt atccgtaaag ctgcactggg gtatgacgta ccgtaagcac
     9661 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
     9721 aatgaacgga ggtcctatga aacgttttag tctggccatt ctggcgctgg ttgttgcgac
     9781 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggttgg
     9841 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcacccga
     9901 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
     9961 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
    10021 acaaaatacc ggtaaacatg aagggccaga aggtaccggg catttaggcg atctgcctgc
    10081 actggtcgtc aataatgacg gcaaagctac cgatgccgtc atcgcgcctc gtctgaaatc
    10141 actggatgaa atcaaagaca aggcgctgat ggtccacgtt ggcggcgata atatgtccga
    10201 tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    10261 acgaggccag cgtaccttgc ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc
    10321 gccagacaat tgccgccagc tcacgggcgg caggttgatg atgacgggca aggctgtcgc
    10381 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    10441 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    10501 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcagc actacacccc
    10561 agcgtaataa ccaacggcga gccagcgcat cctggctgtt actcaactga ctgacgtgat
    10621 gataagtgag cgattcaaat tcgctttcac tcagtgttgg atggcggctt agctgatcga
    10681 caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt acgagcatcc gatcctggac
    10741 gcagaatggc gaacgctagc caagccagcg caacgccaac gatttttgcc agattatcgt
    10801 taagaaaatc agcaaaatca tacaccgggg gattagtgac agcgataaac gaacccataa
    10861 aaacaatcag ttgcccccac agtgcggcaa atttgggcat ctgcaattta agaagctgca
    10921 ttgtcgccag cagtggaaag agaaacagta aaaattgcca cagatcgcta atctggacca
    10981 tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa caccagcgtg cgcatcagca
    11041 gtgacaacga cttaaacggt gctgcgacgg cggagtagag tacgcagcta attgctgcca
    11101 gcgttaatgc attggcaccg gcatcccatt gcgatgcaat actccatgcg ccaatcatca
    11161 tcaacgtaca aaatgtacgc agcccgctcc acatagcttc ggcgttgtcg gtatgacgag
    11221 ccagagcgga tgtccgtgcg agtctggcat gatcatctac accgctttgc agacgatgta
    11281 attcctgact actttgcaga taaaggcggc aaaaataacg tagtcgctgc cagaaggcga
    11341 cgtgccggta gtcggcgacg ttggtcgggc gtagcggggc gataatacgt gcgacggtgt
    11401 aagcatctgt ttgcgaactg gcgagcgccg tcagcaattg ttcgagaatt tctcgcgtgg
    11461 cacttggcgg tgagggccag ttgagcaaca tacgtcgcag gctggagatg acactggtca
    11521 tacgtaattg ctggtggagt agcgcattaa ggcgcgcgtt ttgctggcga aaacgatagt
    11581 ggctccagaa agcctggata cgcagcaaat tcatggtcag tatctgccca atcaccccct
    11641 catgtgctgc acgaatggca tcgtttgttt caggctgcca gagtaaactg gcatgttcca
    11701 gtaatcgggc gtgcatgttt ttcaatgcag ttaaaagggc agtagcatcg gaactgctag
    11761 gcaggatcat catcatcatg ccgccgcaca aaataccgac tatcacctcg caaacgcgcg
    11821 cctgagcgat atcccacagc tggctggcct cagtaatatt aaccatcgga aaggcgatga
    11881 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
    11941 aggcctaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
    12001 tgagcgtatg tccggcaaga agcagtgccg caatggctcc cagtaaactg ccagcgatgc
    12061 gtccgaggct tttgctgatg acaccgccaa cggtgggaaa gctaaccact gcagccgagg
    12121 tcatcgccca atagggttca tccagattca aataataggc aaccgtcagc gccagacaca
    12181 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagggca
    12241 aattgcgcaa ggaccatgac gatgcgttca ttattgacct acggcaatgc tacaggtggt
    12301 gcccgacacc agcgtgacat cgcctggaac tttatccagc gcaaagcgga cgggaacgcg
    12361 ttgggcgaga cgaacccagg ggacgttagg tttcacatcc ggaataaggc ttgagtcact
    12421 ttcaacgctc tgatcataaa tcgcccgacc gatactggaa acgtgaccct gtaacgtttt
    12481 gttatcacta tagagggtaa tttgtgctgg tgcaccttcg cggatatggc gcaacttggt
    12541 ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg acaagggcaa acagcggttt
    12601 cccggtgtca gcgtaatcgc cgatgcgggt agtgaggtta gtcacccatc cactcaccgg
    12661 ggcgcgaatt tccgtttgcg ccagttgcca ttgtgtctgt ttcaacgtcg cctgtgcgac
    12721 attaacactg gcctgcatcg ctttaacatt gaggttagcg gtatcgagtt cttcggcaaa
    12781 aataaaattt tgtgagagat gacgacgacg attcgcttcg ttattggctt ttgcgaggtc
    12841 agattgcgct ttcgccagct gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt
    12901 atcgatcgtg agcagaagtt cccccgcgtt aaccagttgg ttgtctttta tattcagctc
    12961 aacaatacgt cccgacacct gtggggtgat agaaacctgt tctgcgcgta ttttcccgtc
    13021 gcgtgtccac ggagattgca tgtaataatt ccacagccac catccggcaa gaaccgcaac
    13081 tatcgctaca atgattgttg agaaatactt aattgtttta attgacatag ttaccacgca
    13141 atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag
    13201 ggatgccaga tgtcaccggc gtagatccag ccacgaagca ggcggtgtac gacaagccag
    13261 atgacgaatc caaacgcgaa tgctttgaaa aacggaggaa aatagacgga cgcaccgaac
    13321 accagatctt gtaagggcaa tcctgttgca ttgagcataa acttcacaaa gtagattcct
    13381 ttacgaccga atatattgcg ttagattaat aaatattctt taagtgcgaa aaatttacgc
    13441 gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag caatacattt gttttagcaa
    13501 tacaattgct gcacactatt ctaaaagccg cataatatct tagcaagcta attataagga
    13561 gatgaaattg gaatcgccac taggttctga tctggcacgg ttggtgcgca tatggcgtgc
    13621 tctgatagac catcgcctga aaccgctgga gttaacacaa acccattggg ttacgttaca
    13681 caatatccat cagttacctc cagaccagtc gcaaattcaa ctggcaaaag cgattggcat
    13741 cgagcagcca tcactggtcc gtactctgga ccaactggaa gaaaaagggt taatttcgcg
    13801 tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc
    13861 actgatcagc gaaatggaag ctgttattaa caaaacccgc gcggaaatat tacatggcat
    13921 ctccgcagag gaactggagc aactgattac gctcatcgca aaacttgatc ataatatcat
    13981 tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc
    14041 aaacttattt agcgcggaga aacggtcacc tgactgccat tgctggccag tacgacacgt
    14101 tggcccggag agaaacgagt gttgccttgt ttctgtacca ccatgatggt attaccatcg
    14161 tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg tacgccctga
    14221 ccagctacgc cacctgcaac agcgcctgct gcagtagcca gagaacgccc ggttccgcca
    14281 ccaacagtat tccccaggaa accaccaaga acagcaccgc caattgcacc gataacgttg
    14341 gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca
    14401 ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt gtcgttatta
    14461 acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt aatcattgaa
    14521 aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa gtatatggca
    14581 tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg aaagtaggat
    14641 caaccaatta taaacaaaac attcatgatg attattgatt tgaagataat gttttactac
    14701 caacggtaaa aatcggtcat tccgcatggt attcacgaaa agtttatgtt tgagtggtgc
    14761 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
    14821 caggcaccag ccttgatggt gttgatgttg tgttggcgac aattgatgaa caccgggtcg
    14881 cacagctggc aagcttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
    14941 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
    15001 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
    15061 tagttgcgat cggctgtcac ggtcaaaccg tctggcatga accgacgggc gtggcaccac
    15121 acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt acggttgtcg
    15181 gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgttg gtacctgcgt
    15241 tccatcatgc cttgctggct cacccaaccg agcgacgaat ggtgctcaat attggcggca
    15301 tcgccaatct gtcactgctc attcctgggc agccggttgg cggctacgat accggtcctg
    15361 gtaacatgct gatggatgcc tggatctggc gtcaggcagg taaaccttac gataaagatg
    15421 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
    15481 cgtatttctc gcaacctgca ccgaaaagca ccggacgcga atactttaac tacggctggc
    15541 tggagcgcca tttacgccat tttccgggtg ttgatccccg tgatgttcag gccacactgg
    15601 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
    15661 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
    15721 tgctaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
    15781 cattggcttt cgcctggctt gcctggcgga cgctggcggg attaccagga aatctgcctt
    15841 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccccgcc aactcgtgat
    15901 atcagaatta actgaatttt ttcttatcgt gtctgccgtt acacttggaa gattaaggag
    15961 ggtatttgcc ctccggacca ggaaagtctt cgggatatga ctatgaaaaa actgttaatt
    16021 atcattttgc cggtccttct ctccggttgt agcgccttta atcaactggt tgagcgaatg
    16081 caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
    16141 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcctt
    16201 tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa aggtgaggaa
    16261 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccacag
    16321 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
    16381 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
    16441 aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg aacgttggct
    16501 ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg ctaccgtgga
    16561 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
    16621 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
    16681 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
    16741 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
    16801 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
    16861 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
    16921 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
    16981 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
    17041 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
    17101 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
    17161 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
    17221 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
    17281 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
    17341 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
    17401 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
    17461 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgcgga
    17521 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
    17581 ttcctgcgcg atataggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
    17641 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
    17701 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct gcaaattggt
    17761 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
    17821 aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac caaatttggt
    17881 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
    17941 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
    18001 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
    18061 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
    18121 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
    18181 gcggacttcg aacagttggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
    18241 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
    18301 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
    18361 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
    18421 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
    18481 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccact ctcacgttgt
    18541 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
    18601 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
    18661 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
    18721 acacacctgt gatgccgtct taagtggcta tctgggatcg gcggagcagg gtgaacatat
    18781 cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc
    18841 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
    18901 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
    18961 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
    19021 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
    19081 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cactggtgga
    19141 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
    19201 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga
    19261 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
    19321 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
    19381 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
    19441 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
    19501 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
    19561 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
    19621 ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac
    19681 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
    19741 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
    19801 tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc
    19861 acctgcccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
    19921 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
    19981 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
    20041 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
    20101 taaaagagtt tccctgaatt ttacgctaca gcagctttcg ccgctttgtc tgcagcgtca
    20161 tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca
    20221 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg
    20281 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
    20341 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
    20401 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
    20461 cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc
    20521 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
    20581 ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
    20641 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
    20701 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
    20761 aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
    20821 ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
    20881 gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca
    20941 acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa
    21001 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
    21061 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct
    21121 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
    21181 agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
    21241 aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca
    21301 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
    21361 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
    21421 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
    21481 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
    21541 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
    21601 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
    21661 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
    21721 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
    21781 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
    21841 aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttga cggcgatatg
    21901 ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
    21961 atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt
    22021 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
    22081 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
    22141 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
    22201 tatcaatggg gtaatcgatg ttaccccttt tcttcagatg tcaactttct ctttgtattc
    22261 acaaagatct tcaataatac aagagccaca gcggggtttg cgggcaatgc aggtataacg
    22321 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag
    22381 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
    22441 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
    22501 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
    22561 ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct
    22621 gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccca gttcaagcat
    22681 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgacatc
    22741 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
    22801 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc
    22861 tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca
    22921 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag
    22981 ccaataaatg cacctggtgg tagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
    23041 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg
    23101 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta
    23161 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
    23221 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
    23281 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
    23341 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
    23401 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
    23461 gcagtggacg tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg
    23521 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
    23581 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
    23641 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
    23701 tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg
    23761 gtgatccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc
    23821 tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc
    23881 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
    23941 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag
    24001 caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgcc
    24061 ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca
    24121 gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt
    24181 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
    24241 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
    24301 aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga
    24361 ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca
    24421 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
    24481 gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
    24541 tgccatagca accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg
    24601 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg
    24661 cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
    24721 cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg
    24781 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
    24841 accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc
    24901 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc
    24961 atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct
    25021 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
    25081 gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac
    25141 cacagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
    25201 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
    25261 tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact
    25321 gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc
    25381 ttgcgtgctt tggcgcgggc aatagc
//
LOCUS       DADKXM010000047        25335 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.047, whole genome shotgun sequence.
ACCESSION   DADKXM010000047 DADKXM010000000
VERSION     DADKXM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.047"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            21..494
                     /locus_tag="JQR05_001902"
     CDS             21..494
                     /locus_tag="JQR05_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5324082.1"
                     /translation="MRDKAMVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGE
                     FYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKE
                     AAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(504..1931)
                     /locus_tag="JQR05_001903"
     CDS             complement(504..1931)
                     /locus_tag="JQR05_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY5324083.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1928..2635)
                     /locus_tag="JQR05_001904"
     CDS             complement(1928..2635)
                     /locus_tag="JQR05_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY5324084.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHLSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     PADYPTCPETLKDMSDL"
     gene            2799..3776
                     /locus_tag="JQR05_001905"
     CDS             2799..3776
                     /locus_tag="JQR05_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY5324085.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3846..4328)
                     /locus_tag="JQR05_001906"
     CDS             complement(3846..4328)
                     /locus_tag="JQR05_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY5324086.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4563..7145
                     /gene="leuS"
                     /locus_tag="JQR05_001907"
     CDS             4563..7145
                     /gene="leuS"
                     /locus_tag="JQR05_001907"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY5324087.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMAERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7160..7741
                     /gene="lptE"
                     /locus_tag="JQR05_001908"
     CDS             7160..7741
                     /gene="lptE"
                     /locus_tag="JQR05_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY5324088.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7741..8772
                     /gene="holA"
                     /locus_tag="JQR05_001909"
     CDS             7741..8772
                     /gene="holA"
                     /locus_tag="JQR05_001909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY5324089.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8774..9415
                     /gene="nadD"
                     /locus_tag="JQR05_001910"
     CDS             8774..9415
                     /gene="nadD"
                     /locus_tag="JQR05_001910"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY5324090.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9439..10050
                     /locus_tag="JQR05_001911"
     CDS             9439..10050
                     /locus_tag="JQR05_001911"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY5324091.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNNRAIGVENA"
     gene            10310..10627
                     /gene="rsfS"
                     /locus_tag="JQR05_001912"
                     /gene_synonym="rsfA"
     CDS             10310..10627
                     /gene="rsfS"
                     /locus_tag="JQR05_001912"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY5324092.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10631..11098
                     /gene="rlmH"
                     /locus_tag="JQR05_001913"
     CDS             10631..11098
                     /gene="rlmH"
                     /locus_tag="JQR05_001913"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY5324093.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11129..13030
                     /gene="mrdA"
                     /locus_tag="JQR05_001914"
     CDS             11129..13030
                     /gene="mrdA"
                     /locus_tag="JQR05_001914"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY5324094.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKIDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKQWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13033..14145
                     /gene="mrdB"
                     /locus_tag="JQR05_001915"
                     /gene_synonym="rodA"
     CDS             13033..14145
                     /gene="mrdB"
                     /locus_tag="JQR05_001915"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY5324095.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14156..15244
                     /gene="rlpA"
                     /locus_tag="JQR05_001916"
     CDS             14156..15244
                     /gene="rlpA"
                     /locus_tag="JQR05_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY5324096.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15383..16594
                     /gene="dacA"
                     /locus_tag="JQR05_001917"
     CDS             15383..16594
                     /gene="dacA"
                     /locus_tag="JQR05_001917"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY5324097.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALDLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16705..16968
                     /locus_tag="JQR05_001918"
     CDS             16705..16968
                     /locus_tag="JQR05_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY5324098.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17069..17710
                     /gene="lipB"
                     /locus_tag="JQR05_001919"
     CDS             17069..17710
                     /gene="lipB"
                     /locus_tag="JQR05_001919"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY5324099.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17969..18922
                     /locus_tag="JQR05_001920"
     CDS             17969..18922
                     /locus_tag="JQR05_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY5324100.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19131..20096
                     /gene="lipA"
                     /locus_tag="JQR05_001921"
     CDS             19131..20096
                     /gene="lipA"
                     /locus_tag="JQR05_001921"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY5324101.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20197..20400)
                     /gene="tatE"
                     /locus_tag="JQR05_001922"
     CDS             complement(20197..20400)
                     /gene="tatE"
                     /locus_tag="JQR05_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY5324102.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20529..21317)
                     /locus_tag="JQR05_001923"
     CDS             complement(20529..21317)
                     /locus_tag="JQR05_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY5324103.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMACYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21410..21793
                     /gene="crcB"
                     /locus_tag="JQR05_001924"
     CDS             21410..21793
                     /gene="crcB"
                     /locus_tag="JQR05_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY5324104.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21847..22056)
                     /gene="cspE"
                     /locus_tag="JQR05_001925"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21847..22056)
                     /gene="cspE"
                     /locus_tag="JQR05_001925"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY5324105.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22231..22791)
                     /locus_tag="JQR05_001926"
     CDS             complement(22231..22791)
                     /locus_tag="JQR05_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY5324106.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23379..24764
                     /gene="dcuC"
                     /locus_tag="JQR05_001927"
     CDS             23379..24764
                     /gene="dcuC"
                     /locus_tag="JQR05_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY5324107.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHNVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24805..25146)
                     /locus_tag="JQR05_001928"
                     /pseudo
     CDS             complement(24805..25146)
                     /locus_tag="JQR05_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6465 a         6299 c         6243 g         6328 t
ORIGIN      
        1 gccaacccaa gaaaacgacg ttgcgtgata aagccatggt gaattatgcc tttgattatt
       61 taagatcacc aggcagtcta ccgttaacca cggcagccac ggagctttcc gcgattcatg
      121 gtcactcaac atcgcaatat cgccttggcg aattttatct tcatggtagc gacggtaaac
      181 cactggatta tacacaggcg agatactggt atgagcaatc agcagaacag gaaaatccgc
      241 gcgcacaaag caaactgggg tggatctacc tgaaaggcct gggagtcaaa cccgataccc
      301 gtaaagcaat tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata
      361 ctttgggttt gatctacaga aatggcactg gtattaatgt taaccattat gaatctcaaa
      421 aatggttaaa actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccggac
      481 ttcccgcaca ttaacgtcga ctgtcacccg aagagatgga gtattttccc tatcaaaccg
      541 gcaaagataa aaatgtagaa aataattttg atgacgccca gacagcccag cccgccatcg
      601 tcaacgggca ttggctgcgg ttgcggctcc ggctcgacgg tttcctgacc ggtaaaattc
      661 gccgcctgaa ttaataatcg acgaataatt aatgcttcca gtggacaacg atcttctgca
      721 tcaaggcggc tcacaatcgg cattaatgat tcttctgtcg gttggctggc ctgcactaat
      781 tgtttggcaa ctaaatataa tggccataag ggatgttctg tatcacgcgt gagtgagatc
      841 cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg aacgccccag cgcagagagt
      901 ttttcatcgc tgagtatttc gtataaatga aaacgcgtac gggcgctgtc gccggggaca
      961 taaagtagcg gttttccctg atcgttaacc agatcacgcg cttcaatcat catcaccact
     1021 aatgggtaat aatcgacccg atgctgacgg caccattgca acattaaagc atccgcctgg
     1081 gtggagggat actcgcgcca gtagtaacaa agttctgcca gcaggctttc gccttctcca
     1141 caatagacgc gttgtgcgta ttcataataa cgcgcatctt cattatcagg ttcagcttcc
     1201 cgccacgacc gggcgtaatc caggagttcg gggattccca ggcggcttgc gctataccag
     1261 cggagtaaat ttctgtgcag ttttgcatca tcggggataa tcactgcgcg atgttgcgct
     1321 aaatacaatg tcagccattc tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa
     1381 tcgatggtgt cattttgcac ggcaacgggc agatgcagaa tggtctggaa gttaaaaaca
     1441 ttccccttcg caatggcata taaaaaggat tccagttctt cttcgtctat ttccccgacg
     1501 ttttcctcct gcaaccaccg caatctttct gctaataaca ccacgcactc gaaggataaa
     1561 tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta attcatcaat ctcttccatc
     1621 gagcaataat ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa acgttcgcta
     1681 tcagaggcaa gtaacgcgcg aaaggcgagc agaatttcat gttctgctac ctcatattct
     1741 tctggttgcc aaacagattt agcaggcgac tgcgcaatcc gtagcgcctc ctcatacgcc
     1801 tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat gaaaggacgg taacagcgcc
     1861 agataagcgc ggcggataat atcgacgtca gtcgtttcct ctatatcgag gatcttccag
     1921 caatttttca taagtcgctc atatccttaa gcgtttccgg gcatgtgggg tagtcagccg
     1981 gtggttgcca ggacaaggta gggtaatacg catcatcact ggcaaataat tgataaaaca
     2041 tatcattgcc ggaatcgtcg tattctttac gcgctaagaa ttcgcgtaat aaaggcagat
     2101 gatcatcttc gggagactgc caatattgcc gaccaaaggt ataaccggca aagtattgcc
     2161 gccagttact ataataacgt agagcccgta gatgaatacg cgactggatc catagacttt
     2221 cttcttcact caaccagtta ttaagtacgc ccattcgact taaaaaaccc atccggacat
     2281 aatcccatgc tttaattcct ccctcaccac agcattcggc tgtgctggcg acaaattgcg
     2341 cataagcctg accttgctca tttaattcag caagatagtc acgccattca catggagaat
     2401 aacgaaacca gcggtgataa aaccctgcca accgagcggc atgaccgttc tcgatcattc
     2461 ggtgaattgt gcgcagcagg tcatcacggt catcaattcc ccatgaggaa gaaaggtcaa
     2521 aaaagcatgt acctaaatac attttggggg ataaatgcga aggtaaacag cattcattgc
     2581 gtatttccat cggagtggaa agtgcaaaca atattttttg cgaccccatg tccatataat
     2641 ttatccttaa acattcattt aatgaatgta aactacattt cgagactcag ttatttatac
     2701 ttttctgacg caacataata cgcataaaat aaggcttacg caaaaatgga acacataaaa
     2761 atataatata ctttatctac aaccataagg aaatgataat gatttttacg tcaagttgct
     2821 gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc
     2881 aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg gacgacgaaa
     2941 aggcctttta ctggctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct
     3001 tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct
     3061 ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt ggctggatac
     3121 tggatcgtgg agaagacccc aactataaag aagcggttac ctggtatcag atagccgcgg
     3181 agagcggaat gtcttatgcg caaaataatc ttggatggat gtacagaaat ggcaacggag
     3241 tcgcacaaga ctatacgctg gcatttttct ggtacaaaca agctgcatta caaggccata
     3301 gttacgcaca agacaatctg gccgatcttt ataaagacgg agaaggcgtt gctcaaaaca
     3361 agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg cacgcccagt
     3421 ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga
     3481 tgtactggta tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc aacatcggtt
     3541 atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta
     3601 cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt atgtatcact
     3661 acggagaagg aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt
     3721 catccggaac cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagaaat
     3781 aaaaacaagg gccggggaat ttcattctgc ccatattctc tttattgctg tgcgaaatca
     3841 gactattacg gctcaaacgg gcgtctctgg aactggtcat aaccacattt cgggcatagc
     3901 gtcagcactt ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt
     3961 cccagcccga ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact
     4021 tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct
     4081 ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg
     4141 gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgatctc ggttcgcgtt
     4201 aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt
     4261 tcgccattgc gcaggcgttc gttcagtgac gcgaccagtt cacggtaata ttgagcaacc
     4321 ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc
     4381 cttgctttgc taatacgccc acaaactctg taaattaatg tacgaataat ccgggcatca
     4441 tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg
     4501 atctgaaaaa ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg
     4561 ccatgcaaga gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg
     4621 agaagcgcac atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta
     4681 tgcttcccta tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg
     4741 acgtgatcgc ccgttaccag cgcatgctgg gcaaaaacgt tctgcagccg atcggctggg
     4801 acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt
     4861 ggacgtacga caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg
     4921 actggagccg cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct
     4981 tcaccgagct gtataaaaaa ggcctggtat ataaaaagac ttctgcggtc aactggtgcc
     5041 cgaacgacca gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg
     5101 ataccaaagt tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg
     5161 acgagctgct caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc
     5221 agcgtaactg gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg
     5281 acaacacgct gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg
     5341 cggtagctgc gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg
     5401 cctttattga cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga
     5461 aaaaaggcgt cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg
     5521 tttgggcagc aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg
     5581 gtcacgacca gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccggtta
     5641 tcctagcggc tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg
     5701 tgctgttcaa ctctggcgag ttcaacggtc ttgaccatga agcggccttc aacgccatcg
     5761 ccgataaact gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact
     5821 ggggtgtttc tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg
     5881 gtaccgtaat gccaaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga
     5941 tggacggcat taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg
     6001 gtatgccagc gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact
     6061 atgcgcgcta cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact
     6121 actggctgcc ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct
     6181 acttccgctt cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag
     6241 cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa
     6301 acggcgaacg taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc
     6361 gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa
     6421 tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat ggctgaacgt tacggcgcgg
     6481 acaccgttcg tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg
     6541 aatccggtgt ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtgtacgagc
     6601 acacagcaaa aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag
     6661 cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtgac cgatgatatc ggccgtcgtc
     6721 agaccttcaa caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac
     6781 cgaccgatgg cgagcaggat cgcgctctga tgcaggaagc actgctggcc gttgtccgta
     6841 tgctcaaccc gttcaccccg cacatctgct tcacgctgtg gcaggaactc aaaggcgaag
     6901 gcgatatcga caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca
     6961 cgctggtcgt ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ccggtggacg
     7021 caacggaaga acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg
     7081 atggcgttac tgtacgtaaa gtgatttacg taccaggtaa actcctaaat ctggtcgttg
     7141 gctaagcgcg ggaggatgcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt
     7201 aatcaccgcc gggtgtggct ggcatctgcg tgacaccacg caggttcctt ccactatgaa
     7261 ggtcatgatc ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca
     7321 gttacgtctg aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc
     7381 cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca
     7441 aacagcagag tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga
     7501 tatctacccg attagcgcca aagtcttccg ttcattcttc gataacccgc aaatggcgtt
     7561 agcgaaagat aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca
     7621 gctgattcgt aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac
     7681 gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg
     7741 atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg
     7801 tatcttttac ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgccag
     7861 gtagctgcgg cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac
     7921 tggaatgcga tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta
     7981 ttgctgttgt taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc
     8041 accggacttc tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg
     8101 caagaaaatg ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag
     8161 acaccggagc aggctcagct tccccgctgg gttgctgtgc gcgcaaaaca gctcaactta
     8221 gaactggatg acgcggcgaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg
     8281 ctggctcagg cactggagcg tttatcgctg ctctggccag acggcaaact gacattaccg
     8341 cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct
     8401 ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc
     8461 agcgagccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg
     8521 aaacgccagt ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag
     8581 aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gtcagccgca gttacgtcag
     8641 gccgtgcaac tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg
     8701 tgggcagagc tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt
     8761 atcgacggtt gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca
     8821 ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac
     8881 aatcatccct aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg
     8941 taaacacatg cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga
     9001 gctaaagcgc aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca
     9061 aggaccggac gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac
     9121 ctggtacgaa tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg
     9181 ttacccactt gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca
     9241 taacccggaa gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg
     9301 gtttaacatc tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga
     9361 tttattgccg gaaccggtat tgacttacat taaccaacaa ggcttgtatc gctgatacct
     9421 gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc atggcgaaac acaagcgaat
     9481 gtcgatggtc tgtacagcgg tcatgcgccc acacccctga ccgcacgcgg tattgagcaa
     9541 gcgcaaaatc tgcatacact actagatgat gtttcctttg acctggtttt atgcagtgaa
     9601 ctggaacggg cacagcatac cgcgcgactg gttctcagtg accgccagct ccccgttcat
     9661 atcatccctg aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac
     9721 ctcatgcaag aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc
     9781 cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg
     9841 cttagtgaat atcagcacta tcagaatatt ttaatcgtca gccatcaggg cgtactgagt
     9901 ctgttaattg ctcgtttaat tggcatgcct gccgagtcta tgtggcattt tcgcgttgac
     9961 caggggtgtt ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat
    10021 aaccgtgcca tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa
    10081 cgaccattga cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac
    10141 acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat
    10201 gatagcccac tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag
    10261 gtatactgac agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa
    10321 agcactccag gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc
    10381 cttagacgtt caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc
    10441 cagccgtcat gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct
    10501 gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga
    10561 tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg
    10621 gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca
    10681 aaccggtttt accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga
    10741 aattccggcc ggcaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg
    10801 tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa
    10861 gccctgggat acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga
    10921 cgtaagtcta ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggctga
    10981 gcagagctgg tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc
    11041 agagagtctg taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata
    11101 agggagcttt gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac
    11161 ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct
    11221 gaccggcgtg cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca
    11281 gacccgctct aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat
    11341 ctacgaccgt aacggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat
    11401 gccggagaaa atcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggtagatct
    11461 gaccgatgac gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc
    11521 tattccggtg aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta
    11581 ccgttttccg ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc
    11641 gttgacccac gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa
    11701 taatgacggc aaactggcca actatgcggc aacgcatgat attggtaagc tgggcattga
    11761 gcgttactat gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa
    11821 ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta
    11881 cctgacgctg gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc
    11941 agctgtggtt gttaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag
    12001 ttatgaccca aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa
    12061 cgacccgaat acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac
    12121 agttaaaccc tatgtggcgg tttcggcatt aagcgccggg gtgatcacgc gcaatacgac
    12181 gctgtttgac ccaggctggt ggcaactacc agggtcggaa aaacgttatc gtgactggaa
    12241 aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt
    12301 cttctatcag gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt
    12361 cggttatggt cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac
    12421 ccgcgaatgg aaacagaaac gctttaaaaa acagtggtat cagggtgaca ccattccggt
    12481 tggtatcggc cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat
    12541 cctgattaat gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg
    12601 caaacaggtg ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta
    12661 ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac cgccctaatg gtacggcgca
    12721 taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa tctggtaccg ctcaggtctt
    12781 cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca
    12841 caaactgatg accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct
    12901 ggagaacggc ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat
    12961 tatgctgggt gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga
    13021 ggaccattaa tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc
    13081 gatcccacaa tgctgctgat tttactggca ttgctggttt acagcgccct ggttatctgg
    13141 agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt
    13201 ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc
    13261 tatctctata tcatctgtat tattttgctg gtggcggtag atgctttcgg agccatctct
    13321 aaaggcgctc aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc
    13381 aaaatagccg taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg
    13441 ttgaagaaca ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca
    13501 cagcctgacc taggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc
    13561 tctggcctta gctggcgtct gattggcgtc gcagtagtgc tggtagcggc gttcattccg
    13621 attctgtggt tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac
    13681 ccggaatcag acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc
    13741 tccggcggat tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc
    13801 cccgaacgcc atactgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc
    13861 attctgattc tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc
    13921 agagcgcaaa ccacctttgg tcgtgtcatg gctggtggct taatgctgat attattcgtt
    13981 tatgtcttcg taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc
    14041 ccactggtca gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta
    14101 atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg
    14161 taagcagtgg ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga
    14221 tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga
    14281 aattagcggg gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca
    14341 gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact
    14401 ggcggcaatc tatgatgccg aaccaggcag taacctgacg gcctctggcg aagctttcga
    14461 tccgacaaag ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac
    14521 taacctggct aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa
    14581 cgaccgcgtt atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac
    14641 caaagttcgt atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat
    14701 ggcttgtacc acagtcgcca aacagactta cgccctgcct gcacctcccg atttaagcgg
    14761 tggcgcggga acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc
    14821 gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc
    14881 tccaacgacc ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc
    14941 cgttgttaca gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc
    15001 cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc
    15061 tcgtgcgcaa cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac
    15121 tcaaaatggc gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag
    15181 taccttgcag caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca
    15241 gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc
    15301 ggaaagtcag atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat
    15361 cacggatgtc gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct
    15421 ggcgctcacc acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa
    15481 tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga
    15541 ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag
    15601 cctgaccaaa atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa
    15661 agaaactgat ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa
    15721 aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg
    15781 cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg
    15841 tagccaggac gcttttgttg gcttgatgaa cagctacgtt aacgcactgg acctgaaaaa
    15901 tacccacttc cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga
    15961 tatggcgctg atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa
    16021 agaaaaagaa tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga
    16081 taacagcctg aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct
    16141 tgttgcttct gcgactgaag gccagatgcg cttgatttct gcggtgatgg gtggacgtac
    16201 ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttctttga
    16261 aaccgttaac ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga
    16321 ttctgatcgc gcttcgttag gggttgataa agacgtgtac ctgaccattc cgcgtggtcg
    16381 catgaaagat ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca
    16441 aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg
    16501 cccgctggtt gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta
    16561 cattaaatta atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt
    16621 ccgtccccat atactaagca tcagtaaaaa aactcccgcc ttctggcggg agttgctatt
    16681 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    16741 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    16801 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    16861 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    16921 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    16981 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    17041 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    17101 cggtcttcag ccttacgagc caatctcccg ggctatgcat gaattcaccg atacccgcga
    17161 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca
    17221 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    17281 cggtgggcag gtgacttatc acgggccggg acaacaggtg atgtatgtgt tgcttaacct
    17341 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    17401 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    17461 cggggaaaag aaaatttgct cactgggttt acgaattcga cgcggttgtt cattccacgg
    17521 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    17581 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    17641 tgctccacgt ttactggaaa atattttagc gctactaaac aatccggact tcgaatatat
    17701 taccgcttaa ttccatacat caatggccca atttacactg ggtcattact cataatacct
    17761 gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcgg
    17821 tgccacgcct tgctccgtaa ctcaccccat tgattaacct attgaaatag ttgattatgg
    17881 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt aaatattatt
    17941 tttccatgaa taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt
    18001 aagcagaaag tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct
    18061 taaccttctg actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa
    18121 agtgcttaat ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt
    18181 ccctgaccca ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca
    18241 tctacatgag tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg
    18301 aagctatgat aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt
    18361 ccttcctgtc atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc
    18421 gcccatcagc gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa
    18481 catgttttgc accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt
    18541 aatttgccgc gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa
    18601 cgctgcgcat gtacttctgt taccggaagg gcaaaatttc agcggtctgc ggcagagagt
    18661 tcaagagatg tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc
    18721 tgcactggtt gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt
    18781 tagtcgttgc tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga
    18841 tttctctatc cactacaaca aattcagcct gcgtgatccc atcctgcacg gagtaataga
    18901 tgttatccgt aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat
    18961 aaaatggcac aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt
    19021 ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct
    19081 attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac
    19141 ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg
    19201 ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca
    19261 agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc
    19321 tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca
    19381 cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg
    19441 cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg
    19501 ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg
    19561 gcggtgccca acactttgcg gattgtatta ctgccattcg ggaaaaaagc ccgcaaatca
    19621 aaattgaaac tctggtgccg gatttccgcg gtcgaatgga tcgtgctctg gatattctga
    19681 ctgcaacgcc cccagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccggc
    19741 aggtacggcc tggcgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag
    19801 cgcatccgga aatcccgacc aagtctggtc tgatggtggg tctgggtgaa accaatgaag
    19861 aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc
    19921 aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt
    19981 tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt
    20041 ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct
    20101 gtaataatta cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat
    20161 cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag agagcttttc
    20221 agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt
    20281 cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact tcttagtccc
    20341 aaacagcaga acgaccagcg ccgcaactac cagcagtttg gtaatactaa tctcacccat
    20401 agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag agaatattaa
    20461 cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta
    20521 aaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagat gggcagttgc
    20581 gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc aggcatttct
    20641 gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgccc gatatttttg
    20701 ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg agcggcaagc
    20761 aacgttgacc agtgatgctc tttgagcggc ccacgaaccc aggcggcagg aagtaccagg
    20821 atttcagctc cctgtaaagc ctgtgccagc gccagctctg gaaagcgtaa gtcataacag
    20881 gccatcagac cgaccttcat cccttccacc tccagtaacg gagctatttc attaccagca
    20941 tcaacatggc gtgattcctg aatggcaaat gcatcataga gatgcagttt ggcataacgg
    21001 gcgacgatgt ttcctgcctg aagtgccacc agcatattcc atgcgcgccc cggcgttgaa
    21061 ggaagatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgt
    21121 ccgaggaatt cgccttccag cagctgtgct gatttaaccg atagatctgc atcatgatca
    21181 tcgcgcgcca gcaatgcttc cggcaggaca aacagcgata cgtcgttttc cgccgcctgc
    21241 gccatcaacg aggcacaaat ctcagcgttc ttttcccaca cagatgtaac agcaaactgt
    21301 cctgcagcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca acgacgatta
    21361 ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag tgttacaact
    21421 tcttttagca gtttttattg gcggtggtac gggaagcgta gcgagatggc tgttaagtat
    21481 gcgatttaac ccgctgcatc aggcgattcc gttggggacg ctggcagcaa acctgattgg
    21541 ggcattcatc ataggaatgg gattcgcctg gttcagcagg atgacgaaca ttgatccagt
    21601 gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct caacattttc
    21661 ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc tgggcattac tgaacgtttt
    21721 cgtcaacctt ctggggtctt ttgccatgac cgcactggca ttctggctgt tttcggcctc
    21781 aaccgcacac taaagaccaa aaaaaacccg ctgattaagc gggttttgaa ttcttgctga
    21841 cgtatcttac agagcgatta cgtttgcagc agaagggcct ttggcaccgt tagtgatttc
    21901 gaactctacg cgctgacctt cagcaagagt tttaaaacca ttagtctgga ttgcagagaa
    21961 gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg aaaccgaatc ctttggactc
    22021 attaaaccac ttaacgttac ctttaatctt agacatcaaa attaccttta catgaaaaat
    22081 agacacaaat gctgtgtcgg ttatcagtac accaattgtg gtacgctttg tccagtgcaa
    22141 ctttactaaa aagtgataaa agtcgcgtta tttttgatgt tgcttatttt agttattgat
    22201 tttaaagaag ttactaaaac ttcatttgtc tcaaaactga aagcgcatcc aggcaaagta
    22261 cacattgcca ttgttgtagg tacccggaat gtaggtcatc tgaaaagtca ctgggccata
    22321 acccacggag gccaatggca gtagaaccgg gagagggatg taattccagt tatcgcgtgc
    22381 cgttacgcca gcggtgaatc ccagacctaa atgaaaattt tcatccgcca gcggtcgcca
    22441 ggtactttcc catccgtatc cggcaatcgg ttcccatttg ttccacgagt ccttaaatgc
    22501 catggcatac aggccatgcc agtttccttt ttcatcccaa cgcgacaggc caaaaccgcc
    22561 accccacggt cgctcgttat agcgatcggt tttttctttg tcgtaagcga aacgtgcatg
    22621 ccaggtgatg gcaggaatat ataaatcata atgttcaggc tgttgccagg tttgtgcaat
    22681 attttctcta aacgttgtca tcctctcatc tgcgttagca aaaactttac caacgctgat
    22741 taactgaata aaaacaaagg aaaagatagc gacatattta ctcacgttca tttgtgacca
    22801 taaaacattt atcaaaaatc tactactagc atagcaaagc tacaattaac ataaccttaa
    22861 tagacccaac ataaagaata atctgaatag ctgctctatt tatttccgct tgttcaagtt
    22921 aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt ctgcgatggg aatagtcaaa
    22981 aaagaaaaac caagtctttt ttgatgacaa aatgcaatca aggaaaaatt attttatttt
    23041 ttaacgagat acaacaatca tcttaacgaa gtatataata tatatcgtca acccatgctc
    23101 atattagatt attttttgag cgcacagccg cagctaacac cattgcaatt aacaaatttg
    23161 catcaatccg ccatcaattt acacacatta ttatgtgata attgccaacc gctaaatatg
    23221 cgttttgtta tctatgtata aaaacagcaa cttcaatgtc ttaatggcag tttttcttga
    23281 ttttaatcag cattcatcgc caatttattg ggcatatttt ttccttaagc tttaggaatt
    23341 ttttattatt tactttgggc ctggagacag gaaaaattat gctgacattc attgaactcc
    23401 ttattggggt tgtggttatt gtgggtgtag ctcgctacat cattaaaggg tattccgcca
    23461 ctggtgtgtt atttgtcggt ggcctgttat tgctgattat cagtgccatt atggggcaca
    23521 atgtgttacc gtccagccag gcttcaacag gctacagcgc cacggatatc gttgaatacg
    23581 ttaaaatatt actaatgagc cgcggcggcg acctcggcat gatgattatg atgctgtgtg
    23641 gctttgccgc ttacatgacc catatcggcg cgaatgatat ggtggtcaag ctggcgtcaa
    23701 aaccattgca gtatattaac tccccttacc tgctgatgat tgccgcctat tttgttgctt
    23761 gtctgatgtc actggccgtc tcttccgcaa ccggtctggg tgttttgctg atggcaaccc
    23821 tgtttccggt gatggtaaac gttggtatca gtcgtggcgc agcagctgcc atttgtgcct
    23881 ccccggcggc gattattctc gcaccgactt caggggatgt ggtgctggcg gcgcaggctt
    23941 ccgaaatgtc gctgattgac ttcgccttca aaacgacgct gcctatctca attgctgcaa
    24001 ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta tctggataaa aaagagcaca
    24061 tctctcatga aatgttagat gtcagtgaaa tcaccaccac tgcccccgcg ttttatgcca
    24121 ttttgccgtt cacgccgatc atcggtgtac tgatttttga cggtaaatgg ggtccgcaat
    24181 tacacatcat cactattctg gtgatttgta tgctgattgc ctccattctg gagttcctcc
    24241 gcagctttaa tacccagaaa gttttctctg gtctggaagt ggcttatcgc gggatggcag
    24301 atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg ggtattcgct caggggctta
    24361 gcaccatcgg ctttattcaa agtctgattt ctatcgctac ctcgtttggt tcggcgagta
    24421 tcatcctgat gctggtattg gtgattctga ccatgctggc ggcagtcacg accggttcag
    24481 gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa actggcgcac tcttccggca
    24541 ttaacccggc gtatttgact atcccgatgc tgcaggcgtc aaacctgggt cgtaccctat
    24601 cacccgtttc tggcgtagtc gttgcggttg ccgggatggc gaaaatctca ccatttgaag
    24661 tcgtaaaacg cacctcggtg ccggtgcttg ttgggctagt gattgttatc gttgctacag
    24721 agctgatggt gccaggaacg gcagcagcgg tcacaggcaa gtaaatagta atgccggagg
    24781 caagtttcct ccggctttat catgtcaccc actgtggtat atgcgttgtg gtctgccaac
    24841 tttgccgtga acaatttcag caataatcag atggcggctg gcgcaatatt caagataacg
    24901 cctggcagtg gtgcggctga tggtcagtgc ctgcgccacc gtttctgccg tatgttgcac
    24961 accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc aaggggtcaa tgccggtcgg
    25021 cagttcgtct ttgggttcac cgcgagcata ggcattaaac atctcatcaa tttgcttctg
    25081 gctggcgcta tcaatacttt ccagcatatg tttacgctgg cggaaacggg ttagcgtttg
    25141 ccccagtgta ttgattcact tgaagtacga aaaaaccggg agaacattgg attattcggg
    25201 atctgatggg attagatttg gtggggcttg caagcctgta gtacaaattt taggcgttaa
    25261 ccaatgaaac gcgaaagata gtaaaaaatt gcttttgttt cattgaaaaa acgaaaaaca
    25321 aaaacactat aaatc
//
LOCUS       DADKXM010000048        25134 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.048, whole genome shotgun sequence.
ACCESSION   DADKXM010000048 DADKXM010000000
VERSION     DADKXM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.048"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..403)
                     /locus_tag="JQR05_001929"
     CDS             complement(<1..403)
                     /locus_tag="JQR05_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY5324108.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="JQR05_001930"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="JQR05_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY5324109.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="JQR05_001931"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="JQR05_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY5324110.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="JQR05_001932"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="JQR05_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY5324111.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="JQR05_001933"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="JQR05_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY5324112.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="JQR05_001934"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="JQR05_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY5324113.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKLGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="JQR05_001935"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="JQR05_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY5324114.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="JQR05_001936"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="JQR05_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY5324115.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="JQR05_001937"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="JQR05_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY5324116.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="JQR05_001938"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="JQR05_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY5324117.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="JQR05_001939"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="JQR05_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY5324118.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="JQR05_001940"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="JQR05_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY5324119.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="JQR05_001941"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="JQR05_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY5324120.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="JQR05_001942"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="JQR05_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY5324121.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="JQR05_001943"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="JQR05_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY5324122.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="JQR05_001944"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="JQR05_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY5324123.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="JQR05_001945"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="JQR05_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY5324124.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="JQR05_001946"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="JQR05_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY5324125.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="JQR05_001947"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="JQR05_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY5324126.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="JQR05_001948"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="JQR05_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY5324127.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="JQR05_001949"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="JQR05_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY5324128.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="JQR05_001950"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="JQR05_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY5324129.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="JQR05_001951"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="JQR05_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY5324130.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="JQR05_001952"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="JQR05_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY5324131.1"
                     /translation="MKVRASVRKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="JQR05_001953"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="JQR05_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY5324132.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="JQR05_001954"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="JQR05_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY5324133.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="JQR05_001955"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="JQR05_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY5324134.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="JQR05_001956"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="JQR05_001956"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY5324135.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="JQR05_001957"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="JQR05_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY5324136.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="JQR05_001958"
     CDS             14543..14911
                     /locus_tag="JQR05_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY5324137.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="JQR05_001959"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="JQR05_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY5324138.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /locus_tag="JQR05_001960"
     CDS             15403..15621
                     /locus_tag="JQR05_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAY5324139.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="JQR05_001961"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="JQR05_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY5324140.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGGIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="JQR05_001962"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="JQR05_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY5324141.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="JQR05_001963"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="JQR05_001963"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY5324142.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="JQR05_001964"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="JQR05_001964"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY5324143.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="JQR05_001965"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="JQR05_001965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY5324144.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20493..21617
                     /gene="dprA"
                     /locus_tag="JQR05_001966"
     CDS             20493..21617
                     /gene="dprA"
                     /locus_tag="JQR05_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY5324145.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21589..22062
                     /locus_tag="JQR05_001967"
     CDS             21589..22062
                     /locus_tag="JQR05_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY5324146.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22091..22633
                     /locus_tag="JQR05_001968"
     CDS             22091..22633
                     /locus_tag="JQR05_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY5324147.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22638..23210
                     /gene="tsaC"
                     /locus_tag="JQR05_001969"
     CDS             22638..23210
                     /gene="tsaC"
                     /locus_tag="JQR05_001969"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY5324148.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23215..24033
                     /gene="aroE"
                     /locus_tag="JQR05_001970"
     CDS             23215..24033
                     /gene="aroE"
                     /locus_tag="JQR05_001970"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY5324149.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEEVAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24030..24287
                     /locus_tag="JQR05_001971"
     CDS             24030..24287
                     /locus_tag="JQR05_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY5324150.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24263..24817)
                     /locus_tag="JQR05_001972"
     CDS             complement(24263..24817)
                     /locus_tag="JQR05_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY5324151.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6424 a         5985 c         6547 g         6178 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgcc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatt agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aactaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactagtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcattgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggcgggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagctg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg caatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgcta aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt acaaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg taccaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctacaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg actggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcctgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcagaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgttac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtgt ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcag
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggtttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaaca tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctagaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca gcgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagtat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gccatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa cctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcacgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 cggttgctta ccgccagaac ttgagcaacg gagagaagca attcagcttc tggatattct
    14761 caaaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggattgag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgctcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactga
    15361 caggattaca ctcgcgccgt taaataacca actggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaagga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgagtag agaaaaataa gaaagggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatacc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcattgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgccc ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtcg cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc tgattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aggctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc tggatggcga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccaagatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagccgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taatacgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac gggtattaaa ctcggctaat aactcttctt gccgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcggaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgct cggcaagacc gggcttagaa gagtggacta tcagaccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gagttcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcagg ttcaggatgc catcaccagt cgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ccttcaattt ccagccagct cattggccat
    19141 gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ctcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccaaca tccatttgca taatggttac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcacagcttt aggcagaatt
    19561 aaaccatagg cgacgacgac cataacatca gcctgcaaat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac cttcttcctc agccagaact
    19681 ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat tcgttgttgt ttcagcggtg
    19921 acagataatc cataaacagt ttgccaacca ggtgatccat ctcatgctga atacagatag
    19981 ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc gcggtcaagg gcgcgaattt
    20041 taactttctc tgcgcgcggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct tgcttatggc agggagataa ggatggtcga tacagaaatt tggctgcgtt
    20521 taatgagcat cagcagcttg tacggcgatg atatggtccg tatcgctcac tggctggcaa
    20581 aacagtcgca tattgatgcg gttgtattgc agcaaacagg gcttacattg cggcaggcac
    20641 aacgctttct ttcatttcca cgaaagagta tcgaaagctc actttgttgg ttggagcaac
    20701 ccaaccatca tttaatccct gcggacagcg aattttatcc tcctcaactt caggcgacga
    20761 cagattaccc cggcgcactg tttgttgaag gagaactgca cgcgctgcat tcatttcagc
    20821 ttgccgtagt ggggagtcgg gcgcattcat ggtatggcga gcgatggggg cgattatttt
    20881 gcgaaactct ggcgacgtgt ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg
    20941 gtgtggtgca taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaatg
    21001 gacttaatac cattcatccg cgtcgccatg cccgactggc tgccagtctg tttgaacagg
    21061 gtggcgctct cgtctcggaa tttcccctcg atgttccacc ccttgcttac aatttcccac
    21121 gaagaaatcg cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc
    21181 gcagtggttc gctggtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct
    21241 tgccaggtcc aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg
    21301 cgattcttgt gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt
    21361 tgccagacgc ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat
    21421 ttcctgagct cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac
    21481 gtgccggcca acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat
    21541 ggatcgcagc tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta
    21601 ctaatgtatt tgtttgaaac ctatattcac actgaagctg agttgcgtgt ggatcaagac
    21661 aaacttgaac aggatcttac cgacgcaggt tttgagcgag aagatatcta caatgccctg
    21721 ctatggctgg aaaaacttgc tgattatcag gaagggttgg cagaaccgat gcaactggcc
    21781 tcggatcctc tctccatgcg tatttataca ccggaagagt gtgaacgact ggatgccagc
    21841 tgccgtgggt ttctgctttt ccttgagcag attcaggtgc tcaaccttga aactcgtgaa
    21901 atggtgatag agcgagtgct cgcgctggat aacgcagagt tcgaactgga tgatctgaaa
    21961 tgggtgatcc tgatggtgtt gttcaacatc ccgggctgcg aaaatgcgta ccagcaaatg
    22021 gaagaattac tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc
    22081 ataagttgtt atggcaaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa
    22141 gtgcggggct gaactggtta ttcgatccgg aaaacacggt ccgtttcttg gatgctcaca
    22201 gtatccggcg tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa
    22261 agttctggag gggcaggttt gccccgtatg tggcgcaaat ctggtattac gccagggacg
    22321 ctttggtatg tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga
    22381 taaaccggac gaaacagcaa ttacatgccc ccaatgtcgg acgggccatc tggtccagcg
    22441 ccgctcccgt tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc
    22501 cattaacttc aaaccaatag ctggagaatg ccctgagtgt cattatccgc tactcatcga
    22561 aaagaaaacc gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt
    22621 ttcggcggaa taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag
    22681 atgtcctcaa tgaaaaacgt gtcatcgcct atccaacgga agcagttttc ggtgtcgggt
    22741 gcgatcctga tagcgaaaca gcagtgatga gactgttgga gttaaaacag cgtccggttg
    22801 ataaggggct gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca
    22861 ccatgttgac tgacgtgcag cgtgaaacca ttttttcctg ctggccaggt cctgtcacct
    22921 ttgtctttcc cgcgcctgcg acaacaccgc gctggttgac gggccgcttt gattcgcttg
    22981 ctgtacgagt caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc
    23041 tggtttctac cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc
    23101 gcgcacaatt tggcgcggcg ttcccggttg tgcctggtga aacggggggg cgtttaaatc
    23161 cttcagaaat ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa
    23221 acctatgctg tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa
    23281 tttgctcagc aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat
    23341 ttcatcaaca cactgaacgc tttctttcgt gctggtggta aaggtgcgaa tgtgacggtg
    23401 ccttttaaag aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct
    23461 ggtgcagtta ataccctcaa gcggttagaa gatggacgcc tgctgggtga caataccgat
    23521 ggtgtaggct tgttaagcga tctggaacgt ctgtctttta tccgccctgg tttacgtatt
    23581 ctgcttatcg gcgctggtgg agcatctcgc ggcgtactac tgccacttct ttccctggac
    23641 tgtgcggtga caatcactaa tcggacggta tcccgcgcgg aagaggtggc taaattgttt
    23701 gcgcacactg gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat
    23761 ctcattatta atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg
    23821 ctcattcatc caggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt
    23881 ctggcatggt gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg
    23941 gcacaggcgg ctcatgcctt tcttctctgg cacggtgttc tgcctgacgt agaaccagtt
    24001 ataaagcaat tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacaggg
    24061 aagagtggga cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggtatgcaac
    24121 tgacatgcgc gatctctggc gagagtctgg cgtatcgctt tactggagat acgccagaac
    24181 agtggttagc gagttttcgt cagcatcgct gggacctgga agaagaagcg gaaaacttaa
    24241 ttcaggaaca aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt
    24301 ccttccattt cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta
    24361 acgggcggat ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat
    24421 tttgtgggac cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa
    24481 gtaaaattga gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca
    24541 ccttgtgacc aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg
    24601 aggatttatg agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct
    24661 gtacataatg tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca
    24721 gacgaacgtc accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg
    24781 gaaaaagatc gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt
    24841 tactaatgac tttgggggca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca
    24901 ccgttttgtg tgcgattgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca
    24961 aaaaaatact tgtgcaaaat attgggatcc ctataatgcg cctccgttga gacgacaacg
    25021 tgaaacactt cacaggatgg tcggaacaac gaagagaaaa aatcctgaaa ttcagggttg
    25081 actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DADKXM010000049        25060 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.049, whole genome shotgun sequence.
ACCESSION   DADKXM010000049 DADKXM010000000
VERSION     DADKXM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.049"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(222..572)
                     /gene="ubiK"
                     /locus_tag="JQR05_001973"
     CDS             complement(222..572)
                     /gene="ubiK"
                     /locus_tag="JQR05_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY5324152.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
     regulatory      634..782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            886..1539
                     /gene="ribB"
                     /locus_tag="JQR05_001974"
     CDS             886..1539
                     /gene="ribB"
                     /locus_tag="JQR05_001974"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY5324153.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1800..1970
                     /locus_tag="JQR05_001975"
     CDS             1800..1970
                     /locus_tag="JQR05_001975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324154.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(2028..2801)
                     /gene="zupT"
                     /locus_tag="JQR05_001976"
     CDS             complement(2028..2801)
                     /gene="zupT"
                     /locus_tag="JQR05_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY5324155.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2917..3732
                     /gene="ygiD"
                     /locus_tag="JQR05_001977"
     CDS             2917..3732
                     /gene="ygiD"
                     /locus_tag="JQR05_001977"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY5324156.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3770..4930)
                     /locus_tag="JQR05_001978"
     CDS             complement(3770..4930)
                     /locus_tag="JQR05_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY5324157.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4936..5607)
                     /locus_tag="JQR05_001979"
     CDS             complement(4936..5607)
                     /locus_tag="JQR05_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY5324158.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGSGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5755..7236)
                     /gene="tolC"
                     /locus_tag="JQR05_001980"
     CDS             complement(5755..7236)
                     /gene="tolC"
                     /locus_tag="JQR05_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY5324159.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQMKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7441..8070
                     /gene="nudF"
                     /locus_tag="JQR05_001981"
     CDS             7441..8070
                     /gene="nudF"
                     /locus_tag="JQR05_001981"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY5324160.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8071..8493
                     /locus_tag="JQR05_001982"
     CDS             8071..8493
                     /locus_tag="JQR05_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY5324161.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8518..9345
                     /gene="cpdA"
                     /locus_tag="JQR05_001983"
     CDS             8518..9345
                     /gene="cpdA"
                     /locus_tag="JQR05_001983"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY5324162.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9345..9926
                     /gene="yqiA"
                     /locus_tag="JQR05_001984"
     CDS             9345..9926
                     /gene="yqiA"
                     /locus_tag="JQR05_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY5324163.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9955..11847
                     /gene="parE"
                     /locus_tag="JQR05_001985"
     CDS             9955..11847
                     /gene="parE"
                     /locus_tag="JQR05_001985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY5324164.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11895..12209)
                     /locus_tag="JQR05_001986"
     CDS             complement(11895..12209)
                     /locus_tag="JQR05_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY5324165.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHSYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12240..12821)
                     /gene="mdaB"
                     /locus_tag="JQR05_001987"
     CDS             complement(12240..12821)
                     /gene="mdaB"
                     /locus_tag="JQR05_001987"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY5324166.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13140..13472
                     /locus_tag="JQR05_001988"
     CDS             13140..13472
                     /locus_tag="JQR05_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY5324167.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13518..14867)
                     /gene="qseC"
                     /locus_tag="JQR05_001989"
     CDS             complement(13518..14867)
                     /gene="qseC"
                     /locus_tag="JQR05_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY5324168.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14864..15523)
                     /gene="qseB"
                     /locus_tag="JQR05_001990"
     CDS             complement(14864..15523)
                     /gene="qseB"
                     /locus_tag="JQR05_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY5324169.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15675..16067
                     /gene="ygiW"
                     /locus_tag="JQR05_001991"
     CDS             15675..16067
                     /gene="ygiW"
                     /locus_tag="JQR05_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY5324170.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVENAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            16120..16602
                     /locus_tag="JQR05_001992"
     CDS             16120..16602
                     /locus_tag="JQR05_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5324171.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17148..19406
                     /gene="parC"
                     /locus_tag="JQR05_001993"
     CDS             17148..19406
                     /gene="parC"
                     /locus_tag="JQR05_001993"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY5324172.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19640..20377
                     /gene="plsC"
                     /locus_tag="JQR05_001994"
                     /gene_synonym="parF"
     CDS             19640..20377
                     /gene="plsC"
                     /locus_tag="JQR05_001994"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY5324173.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20452..21864
                     /gene="ftsP"
                     /locus_tag="JQR05_001995"
     CDS             20452..21864
                     /gene="ftsP"
                     /locus_tag="JQR05_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY5324174.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGCPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21975..24194
                     /locus_tag="JQR05_001996"
     CDS             21975..24194
                     /locus_tag="JQR05_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY5324175.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24237..24494)
                     /gene="yqhH"
                     /locus_tag="JQR05_001997"
     CDS             complement(24237..24494)
                     /gene="yqhH"
                     /locus_tag="JQR05_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY5324176.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24545..>25060)
                     /locus_tag="JQR05_001998"
     CDS             complement(24545..>25060)
                     /locus_tag="JQR05_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324177.1"
                     /translation="DDIVSNHGSVLQAVNSENEKTLAALASLQKEQPESFVAELFEHI
                     ADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDMQIERQFIYDNPICFGEESLFS
                     RVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQRREGKWEIADFIRPNSGSLLKQ
                     IEAKTAARLKQ"
BASE COUNT         5911 a         6204 c         6675 g         6270 t
ORIGIN      
        1 aagactatca cttaaataag tgatactggt tgtctggaga ttcagggggc cagtctaaat
       61 atcgattaag taaaacttaa agaatacata acaattatat tttcaattgc aatcattcat
      121 ttttgtgatg atactctgaa tgggattaag cgataatgta atagaaaaca ctgtaaatta
      181 gaggattaat tcataattta cagtgcatta aagacaaatg tttacagcgt tggggggaga
      241 gtctctggat ctggctgctt tttgatctca gtactgcgat tttccagttc gctgattctt
      301 tgttcaagca gcgccagttt ctcacgagtc cgtaacagga cttgcgtttg gacgtcgaac
      361 tcttcacggc ttacaaggtc gaggcgcgtc agctgagctt gtagggtttg gcggattttt
      421 ttctccacat cttccccgaa ctccctgatt cctttaggca ttgattcgtg aacctggcga
      481 gcgatttgct caattttttt cgggtcaatc attgtagttt ccctgtactg ataggtgttg
      541 agtaccattg tagtgcgata agggtaagtc ataaaccaga attatgtgaa gctatgcgtt
      601 gctgccgcta atcattagcg ttatagtgaa tccgcttatt ctcagggcgg ggcgaaattc
      661 cccaccggcg gtaaatcaac tcagttgaaa gcccgcgagc gctttgggtg cgaactcaaa
      721 ggacagcaga tccggtgtaa ttccggggcc gacggttaga gtccggatgg gagagagtaa
      781 cgattctgtc gggcatggac ccgctcacgt tattttggct atatgccgcc actcctaaga
      841 ctgccctgat tctggtaacc ataattttag tgaggttttt ttaccatgaa tcagacgcta
      901 ctttcctctt ttggtacgcc tttcgaacgt gttgaaaatg cactggctgc gctgcgtgaa
      961 ggacgcggtg taatggtgct tgatgatgaa gaccgtgaaa acgaaggcga tatgatcttc
     1021 ccggcagaaa ccatgaccgt tgagcagatg gcgctgacca ttcgccacgg tagcggtatt
     1081 gtttgcctgt gcatcactga agatcgccgt aaacaacttg atctgccaat gatggtagaa
     1141 aataacacca gcgcctatgg caccggtttt accgtgacca ttgaagcggc tgaaggtgtg
     1201 actaccggtg tttctgccgc tgatcgtatt acgaccgttc gcgcagcgat tgccgatggc
     1261 gcaaaacctt ctgatctgaa tcgcccgggc cacgttttcc cacttcgcgc gcaggcaggt
     1321 ggtgtactga cgcgtggcgg tcatactgaa gcgactattg atttgatgac gctggcaggc
     1381 tttaaaccgg ctggtgtgct gtgtgagctg actaatgacg atggcacgat ggcgcgtgca
     1441 ccagagtgca ttgagtttgc caataaacac aatatggcgc tcgtgactat tgaagacctg
     1501 gtggcatacc gtcaggcaca tgagcgtaaa gccagctgaa aaccgctgct taattactgc
     1561 cttaatcaag aaaccgaagt tgtagctggc ttcggttttt attttgccct gctatgcaag
     1621 atgttaattc cgcttctctt tctatgagaa aatttcatta atatcaggca ttctttttca
     1681 ttatgatcaa aatcatataa ttgttccgtt attatgcgac catatgaata tatccgaaaa
     1741 aggatatgtg aaatttatgc gctaaatgtt tagtattgtt aaatattaag ggtaaaagga
     1801 tgtttatcgc ctggtactgg attgtattga ttgctctggt tgtggtgggt tatttcctgc
     1861 atttgaaacg ttattgtcgg gcgtttcgcc aggacagaga cgcactgctt gaagcgcgga
     1921 acaaatactt aaacagtacg agagaagaga cagccgaaaa ggtagaataa ggctttcgtg
     1981 aagctggcag aagttgtcca tgtgctggac aacttctgat gttgttttta accaattccc
     2041 gccgtttgta gcagcactaa actgaatccc atcactgaca ttccacatag tacgccatag
     2101 ctggggttat tattagggtc aatctctttg gcgagcggca ttaattcatc aaccgagagc
     2161 gccaccataa ttcctgcaac cgccgccatg attgccgcca tgaccaccgg ggaaatcatg
     2221 ctaccgagga ttaaccacgc cagcacacca ccaagaattt ctgctagtcc agaaatcccc
     2281 gcccacagaa tcgcggtgcg tttagacccc gttgccgcat aaaccgggcc tgccactgcc
     2341 agaccttcag ggatattgtg caacgcgacg gccagtgcga tgccaaatcc cagctccagg
     2401 ttgctgctcg ccgtgacaaa ggtggcaatc ccttccggga agttatgcag actgatgccg
     2461 agagtgagca gaatggctgt gcgcttgatc gattttggca acggctgcac cgatttttgc
     2521 attaaatcct gcggatgcgc atgcggcaac atgcggtcca ggccaaagta gccaagcaga
     2581 ccgaagataa acattccata acccaacacc ggcgacattc cttcagctgc tagtgcggca
     2641 ggaagcattt ccattaatga gatgagcaac atgatccccg ccgcaaaacc tagcgaaaac
     2701 gccagtaagc ggttcgaggg tttttgtccg agaacgccga gaaacgcgcc aataaacgtg
     2761 gctgcccccg ccagtatggt cagaatgaga ggtactgaca tcgacaactc cttataaatc
     2821 ttcaccttca ggcagatcgt caaaataact gatgatcatc atcattgtta tccgggttct
     2881 tactccgccg aacaagcgta ttgtgaggat atcaaaatga cacctttagt taaggatatc
     2941 atcatgtctt caacacgtat gccagcattg tttttaggtc acggtagtcc gatgaacgtg
     3001 ctggaagata atttgtatac ccgcagctgg cagaagttgg ggatgacatt gccacgcccg
     3061 caagcgattg tggtggtttc ggctcactgg tttacccgtg gaacaggagt gaccgcgatg
     3121 gagacgccgc ccacgattca tgactttggt ggcttcccgc aggcgctgta cgatacgcat
     3181 tatcctgctc cgggttcgcc tgcgctggca cagcgtctgg ttgagctgtt agcgccgatc
     3241 ccggtgacgc tggataaaga agcctggggc tttgaccacg gctcgtgggg cgtgctgatt
     3301 aagatgtatc ctgacgccga tatccctatg gtgcagttga gtatcgacag tagcaaacct
     3361 gccgcctggc atttcgaaat ggggcgcaaa ctcgcggcgc tgcgtgatga aggaataatg
     3421 ttggtcgcca gcggtaacgt ggtgcataac ctgcgcacag tgaagtggca cggtgatagt
     3481 tcaccgtatc cgtgggcgac gtcgtttaat gagtatgtga aagcgaatct gacgtggcaa
     3541 gggccggtag agcagcatcc tctggtgaat tatctcgacc atgaaggtgg cgcgttatcg
     3601 aacccaacgc cagagcacta tctgccgttg ttgtatgtgt taggtgtgtg ggatgggcag
     3661 gagccaatta ccattccggt cgatggtata gaaatgggca gtttaagtat gctgtcggtg
     3721 cagataggct aacaattacc ggatggcgag tgccatccgg tatcgtggct tattcaacaa
     3781 aaatatgtgg ataaaaccga gacatatctt gggtgatcaa cgcacggtct tcacgaatgc
     3841 caattccggc aggttgatcg ttcaccagcc agctaccaat cagcatatag ctgtcgccga
     3901 atttcggtaa cgggtggaat tgctgaacaa tcatcccttc ttcgccatac ggaccttccg
     3961 ctgcttcaat ggttttgccg ttctcaatga tcgacacgtt tgcgccttca cgggagaaga
     4021 tcggtttaac cacatatttt tccatttgcg gatgatcatc ttccgcaaaa taagcgggca
     4081 gcaggttcgg gtgattcggg aacatctccc acagtagcgg tagaagtgcc ttgttggaga
     4141 taatgctctt ccacgccggt tccagccagc gtacgccagc atcttccagc ttggtggaga
     4201 acatttcgcg cagcataaat tcccacggat aaagcttgaa caggttagaa atcacctgat
     4261 cctgtaaatc cgtgaactgg cctttttcgc ctaaacctat ctcgtcgatg tagaggaact
     4321 cggtggcgat ttcagcttcg gttgcgcagt cctgcaaata ctgaatggtt ccgcgatctt
     4381 ccaccgtgtc gcgacagcag gtgagatgca gcaactggaa gccatactgt tcacgcagct
     4441 caacgaagcg atcgatcagt ttttcttgca gactgttaaa ctggtcgctg ccctccggca
     4501 agttaccggc gttaagctga tcttccagcc agatccactg aaagaacgcc gcctcgtata
     4561 gtgacgttgg cgtatcggcg ttattttcca gaagtttagg ttcgccagtg ccatcccacg
     4621 ccagatcaag acgcgaataa agcgatggct ggtgagtcag ccatgattga cgcacaaaac
     4681 tccaggtgtg ttttggtatg cggaatttgg tcatcagctc atcgctggcg atcacttttt
     4741 ccaccacttt caggcacatc tggtgcagtt cggcggtgac ttcttccagc ttttcaacct
     4801 gggcgagggt caacttgtag taagcatctt cacaccagta cggctcgccg tacatggtgt
     4861 gaaaattgaa accgtattcg tgggctttct cacgccagtc cgggcgctcg gtaatactga
     4921 ctctttccat cggtatcagc cacccattga acgagaagag gtgccggttg cgctacgctg
     4981 catagtgctt tgtttggcaa cagattcacc aaaaccgcca cgggtaacgg tagtggtggt
     5041 cgccggtttt ggtgccattg ccgtcttcgg tacggtcatg gtgcggcctg gctgggctgc
     5101 gccatagttt ttacccgtcg cgtcggtata tttaccgtaa gccggactgg ccgggttttt
     5161 cgaggagaac agcggctgct gtgcaaatcc cgcgccgctg cccatcagac gccccatcat
     5221 gtaaccggcc atcagcggca tccagaaact cccgctggat tgctgagcct gcgcctggtt
     5281 ttctggtgcc atgccagcct gggctggtgc ttgctgacac tgaccttcac caaattcagc
     5341 aacacagtct tcacgggtgg cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt
     5401 gttgtacgcg gtggtacatt cggcgctttt gcctgggttt gcagctgaac agtcgtcagc
     5461 attttgatag agagacactg tttcatcact cttttcacag ccagccagca taaaaacagt
     5521 ggcaaccgcg agagcgactg gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc
     5581 gtggcgtatg gattttgtcc gtttcatttt tgtcttccgg gaccagtggt aaatacccat
     5641 cagaatagag gatggctggt cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac
     5701 ccgtatcttt acgttgcctt acgttcagac ggggccgaag ccccgtcgtc gtcatcagtt
     5761 acggaaaggg ttatgaccgt tactggtggt agtgcgtgcg gatgtttgct gaacgacggg
     5821 tgccgggcta tcaggcgcat aaccatcagc aatagcattc tgttccggcg tttgcggggc
     5881 aacgttttcc ggattagtgg aaaccggttt gctcagcgca ttgttcagtg ccagcaaatc
     5941 ctgctcgttc aacgtaccca gggctgactt aatattcagc tgattaatca ggtagttata
     6001 acgcgcattc gccagctctt gcttggcgtt gtacagcgtg gtggtcgcat ccaacacatc
     6061 aacaatggta cgcgtaccga ccgagtagcc cgcttccatc gcgtctaatg agctttgcgc
     6121 ggaaactacg gcttgtttgt aggcgttaat actactgata gaagcattaa tgttgttgaa
     6181 ggaggaacgt acggtttgca cgacgctacg atgtgcgctt tccagttgct cactggcacc
     6241 aacaaagttg tactgtgcct gtttcatctg cgagttaacc attccgccct gataaatcgg
     6301 cagcgagaag ctcaggccaa ctttgttctg gcccatattg ctatcgtcat actgggtacc
     6361 agcggcacca cgggttttcg aaccgctata agaggtgtca gaaatcccgg tagaagccgt
     6421 taaatccaga gtcggtaagt gaccatcctg cgcctggcga atttgctcgc gcgccaggtc
     6481 ctggctcaag cgtgcctgta acagcgacag gttgcgtttt tcggcttctt tcagcagcgc
     6541 gttaaccggc tgtggtttgt cggttttaaa gttttcgaca ttcagcgccg ccagttccgg
     6601 atagtagtta ccggtgatct ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc
     6661 ggtcacttcg ttcgccagca cggtatcgta ctgcgcgcgg gcgttctgca cgtcggtgat
     6721 cgctaccagg cccacgttaa aacgttgggt ggtttgatct aattgacggt agatcgcttc
     6781 tttttgtgcc tgtgtatagg aaagaacgtc aatagcattc aacacgttga aataagcggt
     6841 cgcggtgttg aggatcaagg tttgctgatc ggtctgatat gtgacgtcct gaatccctgc
     6901 tgctttttcc tgcagcgtta acgcacgcca tttcgacata tcaaaaatgg attgagttaa
     6961 ctgcagggac gcactggtcg cgttcgagtt gatgccgttc gcgtcgcggt agccgttgct
     7021 ataggtgtaa tctgcaccta aacctagctg tggcagtaat ggactgcgcg cttcattaat
     7081 tttttcaaag gcagcatcac gatcggcggc agacttacgc aattccgggt tactaaggcg
     7141 tgcttgctga taaacttgca tcaggttctc ggcctggctc aacgaactga acccagaaag
     7201 gctcaggccg ataagaatgg ggagcaattt cttcatttgc attccttgtg gtgaagcagt
     7261 atttagcgcg atcaaactgt aaaattattg cggattctag cagaagccgc taccgcaaaa
     7321 ggttggcgtt acgtgccata gggcgttaat gtgcctcaat ttaacatatg actgctcaaa
     7381 cggcagtcaa acgtcgctga aattcacatt taattcacta ttagtgccag gacattaaca
     7441 atgcttaagc cagacaacct gcccgttaca tttggcaaaa acgatgtaga aattattgca
     7501 cgagaaacac tttatcgcgg ctttttttca ttagatcttt atagatttcg tcatcgtcta
     7561 ttcaacgggc aaatgagtca tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca
     7621 gtcttgctac cctttgaccc agtgcgtgat gaagttgtgc tgattgagca gattcggatt
     7681 gccgcgtacg acaccagcga aaccccctgg ctactggaga tggttgccgg gatgattgaa
     7741 gagggtgaaa gtgtggaaga tgttgcccgt cgcgaagcga ttgaagaggc gggactgata
     7801 gtcaaacgga ccaaaccggt gttaagtttc ctggcaagcc cggggggcac cagtgagcgt
     7861 tcgtcaatta tggtgggcga agtggacgcc acgaccgcaa gcggtattca tggtctggct
     7921 gatgaaaacg aagatattcg cgttcatgtg gtaagccggg aacaggcata ccagtgggta
     7981 gaagagggga aaatcgacaa cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat
     8041 catcaagcgt taaaaaatga gtgggcataa atgaagcgtt acacacctga ctttcctgaa
     8101 atgatgcgcc tgtgcgagat gaacttttca caattgcgcc gtttgttacc gcgcaatgac
     8161 gcacccggcg aaactgtaag ctatcaggtg gcaaacgcac aatatcggct gacgattgtg
     8221 gaatcgaccc gatacactac cctggtgaca atagaacaga ctgcgcccgc gatcagttac
     8281 tggagccttc cgtcaatgac ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt
     8341 tcaagccagc agatttttcg cttcaaagcg cggtatgatt atcctaataa aaagttgcat
     8401 caacgcgacg aaaagcatca aattaaccag tttttagcgg actggttgcg atactgttta
     8461 gcacatggag cgatggcgat tccggtttac tagcgtcgtg aaacctaagg acaccatttg
     8521 gaaagcctgt taacccttcc tctggctggt gaggccagag gaaggatttt acaaattacc
     8581 gatactcacc tgtttgcaca aaagcacgaa gccctgttag gggtaaacac ctgggaaagc
     8641 taccaggcgg tgctggaggc gattcgtcca caccagcacg aattcgacct gattgtcgcg
     8701 acaggtgatt tagcgcagga tcaatcctct gcggcctatc agcatttcgc tgaaggcatc
     8761 gcaagttttc gtgcgccctg cgtctggctg ccgggcaacc acgatttcca gcccgcgatg
     8821 tacagcgcgt tacaggatgc gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa
     8881 tggcaaatcc tgttgctgga tagccaggtg tttggcgtgc cgcacggtga gctgagcgag
     8941 tttcagcttg agtggctgga acgtaaactg gccgatgcgc cagaacgcca tacgttgctg
     9001 ctgctgcatc atcatccgct acctgcgggt tgtagttggc tcgatcaaca cagtctgcgt
     9061 aacgcgggcg aactggatac cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc
     9121 ggtcatattc atcaggagct ggatctcgac tggaacggtc gccgcctgct ggcaacgccg
     9181 tcgacctgtg tgcagtttaa gccgcactgt tccaacttta cgctggatac tatcgcgccc
     9241 ggctggcgta ctctcgagtt acatgctgat ggcacgctga ccaccgaggt gcatcgcctg
     9301 gcggacacac gtttccaacc tgataccgct tcagaaggct actgatgtct acgcttcttt
     9361 atttacacgg tttcaacagc tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc
     9421 tggcggaaca tcaccctgac gttgagatga tcattccgca gttgccgccg tatccttccg
     9481 acgcggcaga actgctggaa tccattgtcc tggaacatgg cggcgattcg ctgggtattg
     9541 tcggttcgtc actgggggga tattacgcca cctggttgtc gcaatgtttt atgctgcccg
     9601 cagtggtggt aaacccggcg gtacgcccgt ttgaattgct gacggactat ctcggtcaga
     9661 acgagaaccc ctacaccggg cagcaatatg tgctagagtc acgccatatt tacgatctca
     9721 aagtcatgca gattgacccg ctggaagcgc cggatttgat ctggctgctg caacagacgg
     9781 gagatgaagt gctggattac cgccaggcgg tggcgtacta cgcttcctgc cgccagactg
     9841 tcatagaagg cggcaaccac gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg
     9901 attttcttgg tctgcaccat ctctgacgat caactcgaat tgctaactta aaccatgacg
     9961 caaacttata acgctgatgc cattgaggta ctcaccgggc ttgagccggt tcgccgccgt
    10021 ccggggatgt ataccgatac cactcgccct aaccatttgg ggcaagaagt tattgataac
    10081 agtgtcgatg aagcactggc gggtcacgca aaacgcgtgg atgtaatctt acatgctgac
    10141 cagtcgttag aagtgattga cgatgggcgc gggatgccgg tggatattca cccggaagaa
    10201 ggtgtaccgg cggttgaact gattctttgc cgtctgcatg caggcggtaa attctctaac
    10261 aaaaattacc agttctctgg cggcctgcat ggcgtgggga tttcggtagt taacgccttg
    10321 tcgaagcgcg tagaagttaa cgtgcgccgc gatggtcagg tttataacat cgcctttgaa
    10381 aatggcgaaa aggtgcagga tttacaggtt gtcggcactt gcggtaaacg caataccggt
    10441 accagcgtgc acttctggcc ggatgaaacc ttctttgaca gcccgcgttt ttctgtttca
    10501 cgcctgacgc atgtgctgaa ggccaaagca gtactgtgcc ccggcgttga gattaccttt
    10561 aaagatgaga tcaacaacac cgaacagcgc tggtgctatc aggacggtct gaatgattac
    10621 ctggcggaag cggtaaacgg tttaccgacg ctgccagaaa aaccgtttat cggtaatttc
    10681 gctggcgata ctgaagctgt ggactgggcg ctactgtggc tgccggaagg cggtgaactg
    10741 ctgaccgaaa gctacgtcaa ccttatccca acgatgcagg gcggtaccca tgttaatggt
    10801 ctgcgtcagg gcctgttgga cgcgatgcgt gagttctgtg aataccgcaa cattctgccg
    10861 cgcggcgtga agctgtcggc ggaagatatc tgggatcgct gcgcctatgt tctgtcggtt
    10921 aaaatgcagg atccgcagtt tgccgggcag accaaagagc gtctctcttc gcgtcagtgt
    10981 gcggcattcg tttcgggcgt ggtgaaagat gccttcatcc tgtggctgaa ccagaacgtt
    11041 caggcggcgg agttgctggc agagatggcg atttccagcg cccagcgtcg tatgcgtgcg
    11101 gctaaaaaag tggtgcgtaa aaagctgacc agcggcccgg cgctgcctgg caaactggct
    11161 gattgtaccg cgcaggacct taaccgtacc gaactgttcc tcgtggaagg tgactccgca
    11221 ggcggatctg ccaagcaggc gcgcgatcgt gaatatcagg cgatcatgcc gctgaaaggt
    11281 aagatcctta acacctggga agtttcttcc gacgaagtgc tggcctcgca ggaagtgcat
    11341 gatatttcgg tagcgatcgg tatcgatcct gacagcgacg atctgagcca gcttcgttat
    11401 ggcaaaatct gtatcctcgc ggatgcggac tctgatggtc tgcacattgc cacgctgctc
    11461 tgcgctttgt tcgtaaaaca cttccgcgcg ttggtgaaac acggtcacgt ttacgtcgca
    11521 ctgccaccgc tctaccgtat tgatctcggg aaagaggttt attacgcgct gacggaagaa
    11581 gagaaagagg gcgtacttga gcaattaaaa cgcaagaaag gcaagccgaa cgtccagcgt
    11641 tttaaaggtc tgggggaaat gaacccgatg caattgcgtg aaaccacgct tgatccgaac
    11701 actcgccgtc tggtgcagtt gactatcgat gatgaagacg atcagcgtac tgacgcgatg
    11761 atggatatgc tgctggcgaa gaaacgctcg gaagatcgcc gcaactggtt gcaagagaaa
    11821 ggcgacatgg cggagattga ggtttaaagg aaagaacatt gcccggtcag gatggccggg
    11881 caaacaaggc aggattaaat ccctggctgc agaatacgga tattcatctc cagcacgtcg
    11941 ccttttacgg cttcgctgta cgccttcatg tgcggggttt gcagatgcgc ttcaaggtgc
    12001 gcgatgcttt cccactgctc aatcatcacg atagaatccg gtgccataga ctggaaactc
    12061 acgccagcag cgcaatccac cattggcgca tagctgtggc aaccttcttc tttcagtacg
    12121 gttggaacga ttttagcaaa ctgatccaat accgcctgac ggtgatgttg accaggacga
    12181 gtacggattt ctgcgattac ggtaagcatg gttaactcct tctaaagcct gagctctagt
    12241 taaccaaaaa tttccacaag atgcttgcga tattcttcag tatagcgggg aacatcaggc
    12301 atttttatca cgtcattagc gataaatgtc ggcagcggtt ccataccgag gaattggttt
    12361 gctttatgga acggcagata cacaccgtca acgccaacgc cgtggaagaa ctgatctttt
    12421 tcggtgaagg cttccattgg tgcgttccag gttagagaaa gcatatattt tttgccctgt
    12481 accaggccgc cggaaccgta ttttttcgac ggatctttgc gggtacgacc atcgctggca
    12541 tacagagtcc cgtgaccttc ggtgaataca tcatcaatat attttttcac tgtccacggc
    12601 gcgcccatcc accagcctgg catctgccag atcaccacat cagcccagag aaagttttgt
    12661 acttccgctt tgacattgta gtcgctgtcg gcgcgaacga tgcggacatc atgcccgagg
    12721 tcgcgcagtg tgccatccgc gacttcggtc agggtgtcgt tcagttgacc attggagtgg
    12781 gcgaattttt tcgcgccgtt gataatcagg atgttgctca ttttttaacc tcaaggtgag
    12841 acgtttgtcg actatagtac gtcacagagc ggtacggtga aattagcaaa atgtgcaaag
    12901 tcttttgcga atttagcaaa agtctgatct agctctgact caccgatgca ggataaatat
    12961 tttctgaaaa tgacaatact gatagccatt cctcataatt cttactcgca ccggaataat
    13021 ttacacgttt ggttcggttt ttttggggat tgaaaatatg gctttgaata agtaaaagat
    13081 gaaaatatca acgtgtcatg ttgataatta taaatggatt tttatgagcg tactgatata
    13141 tgttaaagca gaaaattaaa acaatatttg aagcattgct atatataatg cttacttatt
    13201 ggttaataga ttcttttttt gctttcaata agtatgactg gatgcttgag tcgggaggca
    13261 atatttgcag tataccttcc gtttcaggcg aagatcgtat attgcaagca atgattgcag
    13321 cattttttct tcttacgcca cttattattc ttatcttaag aaaactcttt atgcgggaaa
    13381 tgttcgagtt ttggctatat gtattctcgc tcgttatttg tctggtttgt ggttggtggc
    13441 ttttttgggg acggtttata ttttgctatt agtaaaatct atgagagtcg ttttaacggc
    13501 tctcatagac agagaagtta ccagcttacc ttcgcctcaa atccaccttg ttccgcattc
    13561 ccaaactcaa cattcatgtc atgcagcttg gcgattcgct ggacaatcga tagcccaagt
    13621 ccactgccgg tagcggtttg tccgggtggg cgatagaagc gttcgccaat tcgcgccagt
    13681 gcctctggtg tcacaccggg gccgttatcc ctcacgatga aattatcagc attcagcgtg
    13741 acgtctatca cgctgccctg tggactgtag cgcacggcgt tatccagcaa atttcgcacc
    13801 aacaaactta gcaatagcgg ttgtccggtg cgtttgatgc cgtgggcatt gagtgtcagt
    13861 cgcacgtcaa ttttcgcctg ctgcgccgtg tggtaaatat ccatcaccga cgattgtagg
    13921 agatcttcaa gcgggatctc cgcgacgtcc tgaaggttat ccagtgagtc cagccgcgat
    13981 agcgtgagca gttgatcaac cagacgagta gcgcgatcga tcccggaatg taattggagc
    14041 agtgcttttt tccgcgcctg cggatcatcg tcagagagct gcgcaacttc ggtttgcact
    14101 ttcagcgctg ttaacgggct acgtagttcg tgagctgcat cggaggtaaa gcgtcgttct
    14161 cgaaccatca tcgcatgtgt gcgggcgaac agttgattta gcgactcaac cagtggccgc
    14221 acttcgctgg gtacgccagt cgcgtttagt ggtttttccg agtcagggtc acgcatacgt
    14281 agtgccagcg ccagtttgtt cagcggcgcg agttcacgac ccagtagtac catcatgatg
    14341 attaacataa tcggcagtgc gaccagccac gggatcaatt gcccggcaac aatcgccagc
    14401 gccatgtctt cacggtattc ccattcctgg ccaacaacga tgcgatattt gccatcaggt
    14461 gaggtcatcc agacaaaacg ccaaggatcg tcttcaccga ccagttgccc gtcagcaaaa
    14521 ccttcccgtt gatagctata gggaatatct tctccgttat cgccatcatt aaggaccatt
    14581 ctgccgtcgt gggtaaagat ggcaaaggtc agcgcatcgt catcaacatg accgtgtttt
    14641 aatttatttg gcgtctgtgc catgcgatcc gccgcgttga tttcgttgag atcgagcgta
    14701 cttaaccgtt tggcaaacag catcagttgg gtgtcgaaca attcatcgac gttatccgtt
    14761 gtttgtttcc aggcgacaaa gctggaaagc agccaggtca ccgaggccag aattaaaaag
    14821 attagcgtca gcctgacgcg cagactaaga cgttgggtaa atttcatttc tcacctaatg
    14881 tgtagccaat accatgcacg gtacgaataa aatcactacc gagtttgcgt cgcagatgat
    14941 gcacatgcac ttcaacggca ttactggtga cctcttcgtc ccaggtatac agtttctctt
    15001 caatcagttt gcgcggcagt acccgaccag cgttacgcat cagtaattcc agcagggcaa
    15061 attcttttgg tttcagtgtt aagggttcgc cagccagcgt ggcgatacgt ttgccggggt
    15121 cgagcatgac gttaccgtgg cgcagctcgt tgctggcctg gccgttggtt cggcgcatca
    15181 gagcctccag cctggcggcg acttctatca acgcaaaagg tttacacaga taatcgtcag
    15241 ctcccagacg cagcccttct acacgttccg ctaacgcatc acgcgcggtc aggttcagta
    15301 ccggctcacg ctgacctttc tctcgccatt cgcgcaaaat atcgcgacca tccatgcctg
    15361 gtaaggttaa atccaggatc accgcatcat aaggtgcgct ataaagcgcc tcttttccct
    15421 gacgaccttg tgtaaaccag tcgacactaa aacccatttt actaaggcca gttttgatgc
    15481 cgtcgccaat cagcatgtca tcttctatca gtaaaattcg cattttttca tccctgcgat
    15541 aaccgtattt gctgccagta aaccgtgttg taacgcgctc tgtacagcat taaattttat
    15601 ttttttccct taagaagttg ttaaggacta tcttgttaaa tgctcggaaa cagacattaa
    15661 agggagtaat aaacatgaaa aaattcgcag cagtaatcgc agtaatggcc ctgtgcagtg
    15721 caccggtgat ggcagcagag cagggcggtt tttctggccc atcggcaacg caaagtcagg
    15781 ccggaggatt ccaggggccg aacggcagcg taacgactgt agaaaacgca aaatccctgc
    15841 gtgacgacac ctgggtaacc ctgcgcggca atatcgttga acgcatctct gacgatctct
    15901 acgtgtttaa agatgccagc ggtactatca atgttgatat cgatcacaaa cgctggaacg
    15961 gtgtgacggt gacgccgaaa gatacggttg agattcaggg tgaagtcgat aaagactgga
    16021 attctgttga aattgacgtc aaacagatcc gcaaagtaaa tccgtaattg ttaccgctcc
    16081 cgggacgcgt tcccgggaat aatttcgcag ggaggcaaaa tgacaaacct gacactggat
    16141 gtaaacatta tcgatttccc atcaatccct gtggcgatgt tgccgcaccg ctgtagccct
    16201 gagttgctca actacagtgt ggcgaaattt atcatgtggc gtaaagagac ggggctttct
    16261 cctgttaacc aaagccagac ttttggcgtc gcctgggacg accctgcaac caccgcaccg
    16321 gaagcgtttc gctttgatat ctgcggcagc gttagcgaac cgattcccga taatcgttat
    16381 ggtgtgagca atggtgaact taccggtgga cgttatgccg tggcccgcca cgttggcgag
    16441 ctggacgata tttcacacac gatatggggc attattcgcc actggctgcc tgcaagcggc
    16501 gagaaaatgc gtaaagcacc gattctgttt cactacacca atcttgccga aggggtgaca
    16561 gagcagcgac tggaaacgga tgtttatgtg ccgttggcgt gatggggaat ctttaccgga
    16621 gtgtgagcca aatcactcgc gataagtttg agcgaacaat gtcacaggcg tattgttgtt
    16681 gggcttataa tttctccggt aaggaatacc atgtatacgc gaaatttatt atccggtgcg
    16741 ccgtaagccg tctcgggggt atccatgagc aagccgcact tatggcagga ggcgcggggc
    16801 actggtggag acacagctaa actgggctga accgtatact tacagtcgac tactttgatg
    16861 ctttaggctt ccctggactc gcataacctc aacttctcga atcgcccgga gcggaccccc
    16921 atgccgagtg gtgtggcatg ggcgcaacct tgtgctgccc tctatgccga tcaagtctac
    16981 agcaaagagt tgtatatcaa ggcacattga tgcctttatc gccgtcggag cacgtccggc
    17041 ggcgctattc acgctgatct cctgtgactc gacgcggcag ataatgtagt atctccggca
    17101 atattgcccc tttgaaggct ggcgaataag ttgaggaatc agaattaatg agcgatatgg
    17161 cagagcgcct tgcgctacat gaatttacgg aaaacgccta cttaaactac tccatgtacg
    17221 tgatcatgga ccgtgcgttg ccgtttattg gtgatggtct gaaacctgtt cagcgccgca
    17281 ttgtgtatgc gatgtctgaa ctgggcctga atgccagcgc caaatttaaa aaatcggccc
    17341 gtaccgtcgg tgacgtactg ggtaaatacc atccgcacgg cgatagcgcc tgttatgaag
    17401 cgatggtcct gatggcgcag ccgttctctt accgttatcc gctggttgat ggtcagggga
    17461 actggggcgc gccggacgat ccgaaatcgt tcgcggcaat gcgttacacc gaatcccggt
    17521 tgtcgaaata ttccgagctg ctattgagcg agctggggca ggggacggct gactgggtgc
    17581 caaacttcga cggcactttg caggagccga aaatgctacc tgcccgtctg ccaaacattt
    17641 tgcttaacgg caccaccggt attgccgtcg gcatggcgac cgatattcca ccgcataacc
    17701 tgcgtgaagt agctcaggcg gcaatcgcat taatcgacca gccgaaaacc acgctcgatc
    17761 agctgctgga tatcgtgcag gggccggatt atccgactga agcggaaatt atcacttcgc
    17821 gcgccgagat tcgtaaaatc tacgagaacg gacgtggttc agtgcgtatg cgcgcggtgt
    17881 ggaagaaaga agatggcgcg gtggttatca gcgcattacc gcatcaggtt tcaggtgcgc
    17941 gcgtactgga gcaaattgct gcgcaaatgc gcaacaaaaa gctgccgatg gttgacgatc
    18001 tgcgcgatga atctgaccac gagaacccga cccgtctggt gattgtgccg cgttccaacc
    18061 gcgtggatat ggatcaggtg atgaaccacc tcttcgctac caccgatctg gaaaagagct
    18121 atcgcattaa cctcaatatg atcggtctgg atggtcgtcc ggcggtgaaa aacctgctgg
    18181 aaatcctctc cgaatggctg gtgttccgtc gcgataccgt gcgccgccga ctgaactatc
    18241 gtctggagaa agtcctcaag cgcctgcata tcctggaagg tttgctggtg gcgtttctca
    18301 acatcgacga agtgattgag atcattcgta acgaagatga accgaaaccg gcgctgatgt
    18361 cgcggtttgg ccttacggaa acccaggcgg aagcgatcct cgaactgaaa ctgcgtcatc
    18421 ttgccaaact ggaagagatg aagattcgcg gtgagcagag tgagctggaa aaagagcgcg
    18481 accagttgca gggcattttg gcttccgagc gtaaaatgaa taacctgctg aagaaagaac
    18541 tgcaggcaga cgcgcaagcc tacggtgacg atcgtcgttc gccgttgcag gaacgcgaag
    18601 aagcgaaagc gatgagcgag cacgacatgc tgccgtctga acctgtcacc attgtgctgt
    18661 cgcagatggg ctgggtacgc agcgctaaag gccatgatat cgacgcgccg ggcctgaatt
    18721 ataaagcggg tgatagcttc aaagcggcgg tgaaaggtaa gagtaaccaa ccggtagtgt
    18781 ttgttgattc caccggtcgt agctatgcca tcgacccgat tacgctgccg tcggcgcgtg
    18841 gtcagggcga accactcacc ggcaaattaa cgttgccgcc tggggcgacc gttgaccata
    18901 tgctgatgga aagcgacgat cagaaactgc tgatggcttc cgatgcgggt tacggtttcg
    18961 tctgcacctt taacgatctg gtggcgcgta accgtgcggg taaggctttg atcaccttac
    19021 cggaaaatgc ccatgttatg ccgccggtgg tgattgaaga tgcttccgat atgctgctgg
    19081 caatcactca ggcaggccgt atgttgatgt tcccggtaag cgatctgccg cagctgtcga
    19141 agggcaaagg caacaagatt atcaacattc catcggcaga agccgcgcgt ggcgaggatg
    19201 gtctggcgca actgtacgtt ctgccgccgc aaagcacgct gaccattcat gttgggaaac
    19261 gcaaaattaa actgcgtccg gaagagctac agaaagtcac tggcgaacgt ggacgccgcg
    19321 gtacgttgat gcgcggtttg cagcgtatcg atcgtgttga gatcgactct cctcgccgtg
    19381 ccagcagcgg tgatagcgaa gagtaatatg ccggggaggg cgctgtttcc tcccgctagc
    19441 aaggaacgcc ggatgaaacg ttaagtcgtc atccggcagc gtattcaacc cccggaaacg
    19501 ataaaaattc ccttgaaacc gttgtttatt catgcgttgc gattaacaat acgcttttcc
    19561 agagagcggc ttttaacaat gccctaacct gatttcaggt gacgtacaat gccagtctct
    19621 cagaccttca gagggtgtta tgctatatat ctttcgtctt attattaccg tgatttacag
    19681 catcttagtc tgtgtattcg gctccattta ctgccttttc agcccgcgta acccgaaaca
    19741 tgtggccacc tttgggcata tgtttggccg tcttgcgcca ctgttcggcc tgaaagttga
    19801 gtgccgcaaa cctgcagatg ctgaaagcta cggcaatgct atctatatcg ctaaccacca
    19861 gaacaactat gacatggtga cagcatcgaa cattgtgcaa ccgccgacgg tgacggtagg
    19921 taaaaagagc ttgctgtgga tccccttctt cgggcagttg tactggttaa ccggcaactt
    19981 attgatcgac agaaacaatc gcactaaagc tcacggcacc attgcggaag tcgtgaatca
    20041 tttcaaaaaa cgccgtattt ccatctggat gttcccggaa ggaacccgca gccgtggtcg
    20101 cggcctgcta ccgttcaaga ctggagcatt tcacgcggca attgcggcgg gcgtcccgat
    20161 tattcccgtg tgcgtctcta caacttcgaa taagattaat cttaatcgac tgcacaacgg
    20221 tctggtgatt gtcgaaatgc tgccgccaat tgacgtcagt cagtatggca aagatcaggt
    20281 tcgtgagctg gctgcccatt gtcgttcgat aatggaacaa aaaatcgccg agctcgataa
    20341 agaagtcgca gaacgcgaag ccgccggaaa agtttaagtc ggcaatctgt atttttgcgg
    20401 ggaacacttt cctgcacggt attactttag ccagttttac atggagcaaa tatgtcactc
    20461 agtcggcgtc agttcattca ggcatcgggg attgcacttt gtgcaggcgc tgttcccctg
    20521 aaggccagcg cagccgggca acagcaaccg ctacccgttc cgccgctact tgaatctcgc
    20581 cgtgggcaac cgctgtttat gactgtacaa cgtgcgcact ggtcatttac gccagggaca
    20641 cgcgcgtcgg tctggggaat caatggtcgt tacctggggc cgactatccg cgtctggaag
    20701 ggcgacgatg ttaagcttat ttacagcaac cgcctgacag aaaatgtctc aatgacggtg
    20761 gccgggctac aggtaccagg cccgctgatg ggcggtccgg cacggatgat gtcgccaaac
    20821 gctgactggg cacccgtact gcctattcgc cagaacgcag ctactctgtg gtatcacgcc
    20881 aatactccca accgcacggc tcagcaggtc tataacggcc ttgccggaat gtggctggtg
    20941 gaagatgaag tcagcaagtc gctgcctatc cccaaccatt atggtgtgga tgattttccg
    21001 gtcattatcc aggataaacg gctggataac tttggtacgc cagaatacaa cgaaccggga
    21061 agcggcggct ttgttggtga tacgctgctg gttaacggtg tacaaagccc gtacgttgaa
    21121 gtctcgcgtg gctgggtgcg cttgcgactg ctgaacgcgt cgaactctcg tcgctatcaa
    21181 ctacagatga gcgatggttg cccgttacat gtgatttctg gcgatcaggg attcctgcct
    21241 gctcctgtat cggtgaagca actttcgctg gcaccgggcg agcgccgcga gattctggtg
    21301 gatatgagca acggcgatga agtgtcgatc acctgtggcg aagcggcgag cattgttgat
    21361 cgtattcgcg gcttctttga gccatccagt attctggttt ctaccttggt gctaacgctg
    21421 cgcccaaccg gccttctgcc gctggtcaca gacagtcttc cgatgcgctt gctgccaact
    21481 gaaatcatgg ctggttcgcc aattcgcagt cgcgatatca gtctgggtga tgacccgggt
    21541 attaatggac agctgtggga cgtcaaccgt attgatgtca ccgcgcagca aggaacgtgg
    21601 gaacgctgga cggtacgcgc ggacgagccg caagcgttcc atattgaagg cgtaatgttc
    21661 cagatccgta acgtgaatgg cgcgatgccg ttcccggaag acagaggctg gaaagatacc
    21721 gtttgggttg acggacaagt ggagctgctt gtttatttcg gtcagccttc ctgggcgcac
    21781 ttcccgttct acttcaacag tcagacgctg gaaatggcgg accgtggctc gattgggcaa
    21841 ctgttggtca atccggtacc gtaatcttca tactcgcccg aggagggcga gtatggaata
    21901 cctttcgatt tctctttcgt tccggcttat aatccccggc ctttgattat ttttttatca
    21961 tttctggaag caaaatgagc tctatctccc tgatccaacc ggatcgcgac ctgttctcct
    22021 ggccgcagta ctgggccgcc tgttttggac cggcaccgtt tttgccgatg tctcgtgaag
    22081 agatggatca acttggctgg gatagctgcg acatcatttt ggttactggc gacgcgtatg
    22141 tcgatcaccc aagcttcggg atggcgattt gcggtcgtat gctggaagcg cagggctttc
    22201 gcgtcgggat catcgcccag ccggactgga gcagcaaaga cgactttatg cgtctgggta
    22261 aaccgaatct gttttttggt gttactgctg gcaacatgga ttcgatgatc aaccgctata
    22321 ccgccgatcg ccgtttacgt catgatgatg cctacacgcc agataatgtc gcgggtaagc
    22381 gaccggatcg cgccacgctg gtttataccc agcgttgtaa agaggcgtgg aaagatgtgc
    22441 cggtgatcct cggcggtatt gaggctagcc tgcgccgtac cgcgcattat gattactggt
    22501 ccgataccgt gcgccgttcc gttctggtag attcgaaagc cgacatgctg atgtttggta
    22561 acggtgaacg tccgctggtg gaagtagcgc atcgtctggc gatgggcgag ccgattagtg
    22621 aaatccgcga tgtgcgtaat accgcaatta tcgtaaaaga ggcgctgcct ggctggagcg
    22681 gtgtggattc cacccgtctt gataccccag ggaaaatcga cccaatcccg catccgtatg
    22741 gcgaagattt gccgtgtgcg gataacaaac ctgttgcgcc gaaaaagcag gaagcgaaag
    22801 ctgtaatcgt gcagccaccg cgccagaaac cgtgggaaaa aacctacgta ttgctgcctt
    22861 ctttcgagaa agtgaagggc gataaagtgc tgtacgccca tgcttcgcgc attctgcacc
    22921 acgaaaccaa cccaggctgc gcccgcgcat tgatgcaaaa acacggtgac cgctatgtgt
    22981 ggatcaaccc gcctgctatc ccgctttcta ccgaagagat ggacagcgtt tttgcgctgc
    23041 cgtacaagcg cgtgccacat ccggcctatg gcaatgcccg tattccggct tacgaaatga
    23101 tccgtttttc ggtcaacatt atgcgcggct gctttggcgg ctgttctttc tgttctatta
    23161 ccgagcacga agggcgcatt attcagagcc gttcggaaga ttcgatcatt aatgagatcg
    23221 aagcgatccg cgacaccgtt ccaggtttta cgggcgtgat ttccgatctc ggtgggccaa
    23281 ctgccaacat gtatatgttg cgctgcaaat cgccacgcgc tgaacaaact tgtcgccgtt
    23341 tgtcgtgcgt ttatccggat atttgtccgc acatggacac taaccacgaa ccgacgatca
    23401 acctctatcg ccgtgcgcgt gatctgaaag gcattaaaaa aatcctcatc gcctcaggcg
    23461 tacgttatga catcgccgtg gaagatccgc gctatatcaa agagttggcg acccatcacg
    23521 tcggcggtta tctgaagatt gctccggaac ataccgaaga agggccgttg tcgaagatga
    23581 tgaagccggg catgggcagc tatgaccgct ttaaagagct gttcgatacc tactcgaaac
    23641 aggcaggtaa agagcagtat ctgatcccgt atttcatctc tgcgcacccc ggtacgcgtg
    23701 atgaagatat ggtgaatttg gcgctgtggc tgaaaaagca tcgtttccgt ctcgaccagg
    23761 tacagaactt ctacccatcg ccgctggcga actcaaccac catgtattac accgggaaaa
    23821 acccgctggc gaagattggt tataagagcg aagacgtctt cgtaccgaag ggcgacaaac
    23881 agcgtcgttt gcataaagcg ttgttgcgtt accacgatcc ggcaaactgg ccgttaatcc
    23941 gccaggcgct ggaagcgatg ggcaaaaagc atctgattgg cagccgtcgc gattgcttag
    24001 tgcctgcgcc aaccattgaa gagatgcgtg aagctcgtcg ccagaaccgc aatacccgtc
    24061 cggcgttgac taaacatacg ccgatggcga ctcagcgtca gacgcctgct acggcaaaaa
    24121 aagcgtcgtc tacgcaatct cgtccggtga atgctggtgc gaagaaacgc cctaaagcgg
    24181 cggttggacg ttaaaaaaaa tccccggcaa ctgacatgct gccggggatt tttttatcat
    24241 tctgcgtaca tacgcaagca gcgcaggcaa tcaaaatagt cctgtgcatc aagacgcgta
    24301 ttggctctgt tagcttcgga tttggcagca tagatttgtg ggcgtagtgc tttcatatcc
    24361 tgctcaagcc gggcgatttt ggcatttaat gtttgtacat tgctcgccag ttgattgacc
    24421 ttttgctcat tgacgcagcc actgagcaag caggttgcca gaacaacagc tcccacggtg
    24481 aaaatcgttt tcatatagtg ttccctttta tttattgttc cggctaattg ttatttaatt
    24541 cagctcattg ctttaatctg gcggcggttt ttgcctcaat ctgcttaagt aggctgccgc
    24601 tgttcgggcg gataaaatca gcgatttccc acttaccttc gcgccgctgt aaaaccagct
    24661 cttgctcttc attgttgcca ttggtcagcg taaaacgaac atgaatgcgg gcggaatcgg
    24721 cggttttctc caggactcga atttcatcaa cgcgtgaaaa tagcgactct tcgccaaaac
    24781 agatcggatt gtcgtaaata aattgccgtt ctatttgcat atcttcatcg ggattattgg
    24841 ccgtcttgaa ggtggtttta tagaaggcat taaccgcctg gcggtaagag tcggaaacca
    24901 cccacgtcca cggccagcta taatcagcaa tatgttcaaa gagttcagca acaaaggatt
    24961 ctggctgttc tttctgcaac gaagctaaag cggccagcgt tttttcattc tcgctattaa
    25021 ctgcttgtaa gacgctgcca tgattgctga caatatcatc
//
LOCUS       DADKXM010000050        24933 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.050, whole genome shotgun sequence.
ACCESSION   DADKXM010000050 DADKXM010000000
VERSION     DADKXM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.050"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JQR05_001999"
     CDS             complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JQR05_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY5324178.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGAAAQGE
                     AVVEDSVLDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JQR05_002000"
     CDS             complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JQR05_002000"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY5324179.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JQR05_002001"
     CDS             complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JQR05_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY5324180.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JQR05_002002"
     CDS             complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JQR05_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY5324181.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JQR05_002003"
     CDS             complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JQR05_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY5324182.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKTLAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRTLKGHSVDEV"
     gene            complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JQR05_002004"
     CDS             complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JQR05_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY5324183.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDVAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JQR05_002005"
     CDS             complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JQR05_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY5324184.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JQR05_002006"
     CDS             complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JQR05_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY5324185.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JQR05_002007"
     CDS             complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JQR05_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY5324186.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JQR05_002008"
     CDS             complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JQR05_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY5324187.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JQR05_002009"
     CDS             complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JQR05_002009"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY5324188.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9556..10359
                     /gene="suhB"
                     /locus_tag="JQR05_002010"
     CDS             9556..10359
                     /gene="suhB"
                     /locus_tag="JQR05_002010"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY5324189.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10504..11358
                     /locus_tag="JQR05_002011"
     CDS             10504..11358
                     /locus_tag="JQR05_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5324190.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11549..12829
                     /gene="csiE"
                     /locus_tag="JQR05_002012"
     CDS             11549..12829
                     /gene="csiE"
                     /locus_tag="JQR05_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY5324191.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLNATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFPPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12821..13960)
                     /locus_tag="JQR05_002013"
     CDS             complement(12821..13960)
                     /locus_tag="JQR05_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY5324192.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGCEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JQR05_002014"
     CDS             complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JQR05_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY5324193.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSTEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15146..16507
                     /locus_tag="JQR05_002015"
     CDS             15146..16507
                     /locus_tag="JQR05_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY5324194.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWVLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16504..17022
                     /locus_tag="JQR05_002016"
     CDS             16504..17022
                     /locus_tag="JQR05_002016"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY5324195.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17022..17342
                     /locus_tag="JQR05_002017"
     CDS             17022..17342
                     /locus_tag="JQR05_002017"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY5324196.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17339..18151
                     /gene="hcaB"
                     /locus_tag="JQR05_002018"
     CDS             17339..18151
                     /gene="hcaB"
                     /locus_tag="JQR05_002018"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY5324197.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTCSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALEQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18161..19357
                     /locus_tag="JQR05_002019"
     CDS             18161..19357
                     /locus_tag="JQR05_002019"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY5324198.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIAIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLLPPPWFWSDQYSDNLQFIGDMRG
                     DDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDEN
                     IALKSL"
     gene            19454..19876
                     /locus_tag="JQR05_002020"
     CDS             19454..19876
                     /locus_tag="JQR05_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY5324199.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19924..20774)
                     /locus_tag="JQR05_002021"
                     /pseudo
     CDS             complement(19924..20774)
                     /locus_tag="JQR05_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
     gene            complement(20786..21868)
                     /locus_tag="JQR05_002022"
     CDS             complement(20786..21868)
                     /locus_tag="JQR05_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY5324200.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGLVGMMAKGRGAKRIIGVDMLPER
                     LAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTADWGR
                     VVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAITHRF
                     SLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21901..22899)
                     /locus_tag="JQR05_002023"
     CDS             complement(21901..22899)
                     /locus_tag="JQR05_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5324201.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIK
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22924..24435)
                     /locus_tag="JQR05_002024"
     CDS             complement(22924..24435)
                     /locus_tag="JQR05_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY5324202.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     KGASKSTLIRKLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLVSAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24458..>24933)
                     /locus_tag="JQR05_002025"
     CDS             complement(24458..>24933)
                     /locus_tag="JQR05_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY5324203.1"
                     /translation="AFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKL
                     IISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAV
                     VDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5879 a         6961 c         6220 g         5873 t
ORIGIN      
        1 tcagacaaat ataaacaaat cctgctcaaa attaaaaatt ctaaccggta aaagatatta
       61 cttaaacatg taaatttact ttcctttaaa aaacaaaaaa ccgccaaaat caggcggtta
      121 tttgttgctg gtccggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
      181 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
      241 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
      301 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
      361 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag
      421 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
      481 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
      541 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
      601 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
      661 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
      721 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
      781 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
      841 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
      901 gctctgcaac ttccgataat atcagcgatt caccgccttc caggatttcc tggctgctca
      961 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
     1021 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     1081 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
     1141 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcacgc
     1201 tatcttcaac cacagcctcg ccctgcgccg ccgcaccagg cacccagacg gtggattcca
     1261 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     1321 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
     1381 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
     1441 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
     1501 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
     1561 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
     1621 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
     1681 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
     1741 gacgccagaa tggttcgttc tcttgtgagg cactcgccag taaacgaccg gcaagcgcat
     1801 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
     1861 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
     1921 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
     1981 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
     2041 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
     2101 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
     2161 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac accggtgctt
     2221 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
     2281 gcccgacagt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
     2341 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
     2401 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
     2461 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
     2521 gcgtgccttt cgggcctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
     2581 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct
     2641 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
     2701 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
     2761 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
     2821 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
     2881 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
     2941 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
     3001 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
     3061 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
     3121 agcttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
     3181 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
     3241 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa
     3301 tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc tgatgaggca
     3361 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgtacgacga
     3421 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
     3481 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
     3541 ttgtcaatga cctgacgctc tgcggcgctt aacagcgcag catcagcagc cagcgcgccg
     3601 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt
     3661 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
     3721 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
     3781 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca
     3841 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
     3901 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
     3961 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
     4021 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
     4081 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
     4141 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
     4201 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
     4261 acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
     4321 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
     4381 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
     4441 tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta atccgctagc
     4501 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
     4561 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
     4621 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
     4681 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
     4741 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
     4801 gcttcagttg cccgcgccgc cagtgttttg aggatgtccg cagaaacgcg caccgggttc
     4861 agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
     4921 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
     4981 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
     5041 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
     5101 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
     5161 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
     5221 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
     5281 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccacgtc
     5341 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
     5401 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
     5461 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
     5521 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
     5581 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
     5641 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
     5701 gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
     5761 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
     5821 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
     5881 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt caggccttct
     5941 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
     6001 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
     6061 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
     6121 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
     6181 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
     6241 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
     6301 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
     6361 ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc
     6421 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt cgccacaggc
     6481 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
     6541 acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
     6601 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
     6661 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
     6721 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
     6781 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
     6841 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
     6901 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
     6961 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
     7021 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccattccgac
     7081 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
     7141 ttgcgcttcg atgcgtacac gcggtttacg acgcacatac agcgcaccga tacctttcgg
     7201 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
     7261 tggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
     7321 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
     7381 ggaaacaagg atggtgtcgt cacgcatcgc cgcttcgagt tctttcaggt cgataatacc
     7441 gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaagt
     7501 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
     7561 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
     7621 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
     7681 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
     7741 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
     7801 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
     7861 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc actccggcct
     7921 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
     7981 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
     8041 cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca
     8101 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
     8161 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
     8221 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
     8281 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
     8341 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
     8401 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
     8461 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
     8521 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
     8581 tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc cgacctacga
     8641 ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa ccactgttat
     8701 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
     8761 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
     8821 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
     8881 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca
     8941 ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg aaccgccatt
     9001 gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata atggcatttc
     9061 tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc caccggggta
     9121 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
     9181 agcgtgcggg agcgtgcgct ggtgcccacc accaagctac aaccggctaa cgcttcgtcg
     9241 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     9301 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     9361 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     9421 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     9481 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     9541 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
     9601 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
     9661 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
     9721 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
     9781 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
     9841 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
     9901 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
     9961 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    10021 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaagctg
    10081 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    10141 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    10201 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    10261 tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc catactggcg
    10321 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatatca
    10381 ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    10441 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    10501 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    10561 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    10621 aaaatttacg gtcccgcccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    10681 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    10741 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    10801 tggttacctg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    10861 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    10921 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    10981 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    11041 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    11101 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    11161 ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga aaagttgtat
    11221 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    11281 ttttccgatc gtcacggcaa tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    11341 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    11401 agacatgcta atccactggt cagaacagtt taaaatgaga aaaattctgt gacgcttgcc
    11461 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    11521 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    11581 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaacgc
    11641 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    11701 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    11761 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    11821 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattccccc ccgccctgaa
    11881 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    11941 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    12001 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    12061 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    12121 tggacgccac tggcagcgtc gggcgctcca gcatgtaccg cctgatgagc cactgtttat
    12181 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    12241 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    12301 tcgtttttat gatgaacagg ggttatgtga tcagctttat atccacctcg cccaggcgtt
    12361 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    12421 gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    12481 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    12541 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    12601 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    12661 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt agcgcggcac tgattattgc
    12721 gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct
    12781 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    12841 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac acgccgtggc
    12901 ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    12961 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgca accctggcga gcagcaatat
    13021 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    13081 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    13141 ccgagatcaa cagcatatcg cgagcactac aacggcggaa aagtttatta ctcagcgcaa
    13201 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    13261 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    13321 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    13381 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    13441 agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc cgataatcaa
    13501 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    13561 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    13621 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcaaccacg
    13681 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tggcagcagt gccagcacgc
    13741 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    13801 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    13861 tccctttaag ccagacgctc cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga
    13921 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    13981 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    14041 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    14101 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    14161 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    14221 aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa ttattcatat
    14281 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    14341 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    14401 tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg ccatccagtt
    14461 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    14521 atggttcgtc aaaaagctcc agataatcaa tctccgtgct ataaacagga tggcgcatta
    14581 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    14641 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcaata
    14701 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    14761 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    14821 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    14881 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    14941 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    15001 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    15061 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    15121 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    15181 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttacca
    15241 actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    15301 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg tcgtggtgcg
    15361 ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    15421 gagttatgca gattgtggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta
    15481 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    15541 gtgtaaatcc cactgggtgc taaacgaagt tccttgtgtg gagagttata aaggactgat
    15601 ttttggcaac tgggatacca gcgcaccggg cctgcatgat tacctgggtg acattgcctg
    15661 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    15721 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    15781 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    15841 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    15901 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    15961 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    16021 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    16081 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    16141 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    16201 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    16261 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    16321 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    16381 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ccgatcttct
    16441 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    16501 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    16561 gaagccagtc tgctggatga ctggaaattt cgtgactggt tggcacagct cgacgaagag
    16621 attcgttaca ccatgcgcac cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc
    16681 cagccgccga cgacctggat tttcaatgac accaaagacc agctggagcg gcgaatcgcc
    16741 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcactcg tcacttaatc
    16801 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    16861 ctactttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    16921 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctagat
    16981 caagcggtga tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    17041 gtcccgttgc ggatgtgccg gagggtgagg ctctccggat agatacctcg cccgtcatcg
    17101 ccttgttcaa cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    17161 cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    17221 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    17281 cttatccagt acacgttgaa ggtggtgata ttttcatcga cttaccggag gcgcagccat
    17341 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    17401 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    17461 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    17521 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgacttgtt ccggcaagct
    17581 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    17641 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    17701 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    17761 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    17821 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    17881 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgagcaaag
    17941 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    18001 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    18061 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    18121 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat
    18181 tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt
    18241 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    18301 ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    18361 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    18421 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    18481 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    18541 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    18601 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    18661 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    18721 cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa
    18781 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    18841 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    18901 cgaggccaac cttgatactg ccaatggcat tgccattgat gaggcttgcc gcacctgcga
    18961 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    19021 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    19081 agggctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    19141 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    19201 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    19261 gcgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    19321 gctgatagat gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc
    19381 aattcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    19441 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    19501 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    19561 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    19621 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    19681 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    19741 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    19801 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accgggccgg gggcaatttc
    19861 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    19921 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    19981 cgcaatgaga tcaccccctt ccgggcgatg atgatcatcc ggcgcatgac tcatcggttc
    20041 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    20101 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    20161 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg
    20221 cgcgagctgg ttaaaatcta gttcctgcgg tagctgctcg caaaactcac cagccagtaa
    20281 ccgctcgcct gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg
    20341 gtaccaaatg gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta
    20401 tccgccgtta aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca
    20461 tacaccagac acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca
    20521 tcgccgtgta gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc
    20581 tgccagacaa aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag
    20641 gcatcggttg ccacaccgct tttgttacca ggacgtaaga gcggcgtcgt atcgcgccag
    20701 aagccttcga ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag
    20761 gtatagatgg tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt
    20821 tgccgctcgc catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa
    20881 tggcgttacg cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga
    20941 acagactggt cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga
    21001 cctcgaattc cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg
    21061 attgcagtgc cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc
    21121 caccgtgggt aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt
    21181 gatccatcac ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga
    21241 tgatccgttt tgcaccgcga cctttcgcca tcatgccgac cagccccaga ccgaccacca
    21301 gcacgttatc gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc
    21361 cgcaactgat aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt
    21421 tttcttccgc cagcagatat tcggcatgac cgccgtcacg ctgccagccg taagccgctt
    21481 ttcctttgcc agtacaagag ataggaaagc cgcggcggca gttcgggcaa aaaccacagc
    21541 cagaaatgtg atacaccagc acgcggtcgc cctctttaaa atggcggcag ccttgcccca
    21601 tcgccacaat ctgcccgcac ggttcatgac cgttgataaa tccctggtat aacggtttat
    21661 cgggtgccgc cgccgtggca cggtgttgat gatagatata gtggacatcg cttccgcaaa
    21721 tcccggagga tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt
    21781 cccgcagatc gacggttgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc
    21841 cctcattaac aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt
    21901 ttagcgtttc gctttactgc ttcgctgggt cagcaagata ttcgccagca ccgccaccac
    21961 gatgatgacg ccgcgtacca cctgctggaa aaaggagtta ataccgagca gcaccagacc
    22021 gttaccgatt agcgtaatca ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc
    22081 gccggaaagt gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt
    22141 tgcggcacct gcgttaccgg aaccgaggcg tgccgccaac aaaatgccgg tcaccgccgc
    22201 taataatccc gaaagggtaa agataagaat gcgcactcga cgaactttaa taccgcacaa
    22261 ctgcgccgcc gtggcattac cgccaacggc aaaaaccgag cgcccgaagg cggtttttcg
    22321 gctaatgaac acaaacagcg caaacaacac catcatgatc agcgcggata ctggcacacc
    22381 gagaaattgt ccgcccagcc agtccagcac ctcgttttcg tcaatcggca ctggcagtgc
    22441 gttggtcata aacagcccca ttccgcgcag ggcgctccac agccccagtg tggcaacgaa
    22501 acttggtacg ttaaacacgc cgcgcagcac cccggcgagc gttcccatca gcgcgcctaa
    22561 cagcaacacc agcagacaag caatcgccaa cggaacttca aattgcagca aaaatgccag
    22621 gcacaccgag acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat
    22681 aatcagcgtc atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat
    22741 ctggttattc aatgagataa agccaggcgc gttcagggag aagaccagat aaagaatggc
    22801 aatcaccacc agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac
    22861 gctcttgccc tgcggcaatg gtaatgacga agcagacatc ttcaggttcc tcggtaaaat
    22921 tcatcagtgc acagacagaa tggcggacat cagctcatcc acattgaccg gcgagtgaaa
    22981 ctcctgcgag aacgtgccgt gctgtaataa caggatgcgg tcacacacca gcggcaactc
    23041 ctccacttca ctggagataa acaccacgct ttttccttcg gcagccagct cacggacaat
    23101 acggtaaatc tgctgtttgg cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa
    23161 aatctggctg gcggcataga cccaacgacc gatcaccact ttttgctgat tgccaccaga
    23221 aagcgtgccg atgggtgttt cgctactggc agccttgacc gtcatccgct gcatcacctc
    23281 ttcggtcagg cggcggatgg tggaccattg cagcacaccg ttggtgctga ttttttgccg
    23341 attggtcagc actgtatttt cgtcaacgcc cagccaggga atgatccccg cttctttgcg
    23401 gttttctggc gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcatgat
    23461 tttctcgccg ttgataacaa tttcgccctg ttcatacgtc tccagcccaa caatcgcctt
    23521 cagcaattca ctgcgccctg cccccagcag gccagcaatg ccaagcactt cgccacgacg
    23581 tagcgtaaag ctgatatcct ccagcttggg cttatggcgt aacgcacgga cttccagtac
    23641 ggcctgatcc acaatttcct gaggggcaac cggcgcaata tcaacgtgat cacgccctag
    23701 cattagcgac acaatatgat gcgtggaggt gttttcgagc atcacatcgc ccgccacctg
    23761 accatcgcgc ataacggtgg cacaggaggc aatgcggcgg atttcttcca tccggtggct
    23821 gacataaatc accgccacgc ccagtgccga catctttttc accgcactga tcaccagttc
    23881 aacttctgca ctgacaagcg agctggtggg ttcatcaaga atgaccacgc gcggctcgcc
    23941 cttcatcacc cgtgcaattt ccaccagttg cttttgcgcc gggcttagcg ttgaaacaag
    24001 ttgttcagga ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat
    24061 ttgcaggtaa tcaatcatgc cgttgcggcg aggccattga ccaaggcaga ggttttctgc
    24121 caccgtcagc ccttccacca gacttaattc ctgataaacc gcacgaaccc ccagttcagc
    24181 ggcacggcga gtcaacgtag cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc
    24241 accgctatcc gggcgttcgc tgccggtaag ctttcgaatg agagtcgatt tgctcgcgcc
    24301 ttttttacct aacagcgcac gaacttcgcc tttattgagc gtgaagttaa cgttatccag
    24361 cgcaacgacg ccggggtaac gcttatttcc tgccaccact tttgctaccg ggactgcctc
    24421 tgttgccgtg aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg
    24481 ttgccagcca ctgtttacca tcttccgttt tggtatagag atcgattggc acctgaatca
    24541 ctttttcacc gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc
    24601 ccattttctt accggaaata tccactaccg ctttcagcac ctgattgttt tccagctcct
    24661 gagcaatttc ggttgtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat
    24721 tacgtaccgc ttttaccgcg ccgagtgtcg caccgcccga ctcccccata atggcgttga
    24781 gatccggcgt ggagataatc agtttttcac caacggaaat cgctttatct aaaacagtcc
    24841 cttcctgatt agcgacaatt tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc
    24901 ctttacgtcg ctgcacacaa acttcaaagg ctt
//
LOCUS       DADKXM010000051        24859 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.051, whole genome shotgun sequence.
ACCESSION   DADKXM010000051 DADKXM010000000
VERSION     DADKXM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.051"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            261..488
                     /gene="feoA"
                     /locus_tag="JQR05_002026"
     CDS             261..488
                     /gene="feoA"
                     /locus_tag="JQR05_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY5324204.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            505..2826
                     /gene="feoB"
                     /locus_tag="JQR05_002027"
     CDS             505..2826
                     /gene="feoB"
                     /locus_tag="JQR05_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY5324205.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2826..3062
                     /gene="feoC"
                     /locus_tag="JQR05_002028"
     CDS             2826..3062
                     /gene="feoC"
                     /locus_tag="JQR05_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY5324206.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3265..4142
                     /gene="rpnA"
                     /locus_tag="JQR05_002029"
                     /pseudo
     CDS             3265..4142
                     /gene="rpnA"
                     /locus_tag="JQR05_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            4215..4484
                     /locus_tag="JQR05_002030"
     CDS             4215..4484
                     /locus_tag="JQR05_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY5324207.1"
                     /translation="MQAVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            4478..4843
                     /locus_tag="JQR05_002031"
     CDS             4478..4843
                     /locus_tag="JQR05_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY5324208.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(4844..5632)
                     /gene="bioH"
                     /locus_tag="JQR05_002032"
     CDS             complement(4844..5632)
                     /gene="bioH"
                     /locus_tag="JQR05_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY5324209.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            5670..6353
                     /gene="gntX"
                     /locus_tag="JQR05_002033"
     CDS             5670..6353
                     /gene="gntX"
                     /locus_tag="JQR05_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY5324210.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRTTRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            6412..6987
                     /gene="nfuA"
                     /locus_tag="JQR05_002034"
     CDS             6412..6987
                     /gene="nfuA"
                     /locus_tag="JQR05_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY5324211.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            7348..8664
                     /gene="gntT"
                     /locus_tag="JQR05_002035"
     CDS             7348..8664
                     /gene="gntT"
                     /locus_tag="JQR05_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY5324212.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8709..10793)
                     /gene="malQ"
                     /locus_tag="JQR05_002036"
     CDS             complement(8709..10793)
                     /gene="malQ"
                     /locus_tag="JQR05_002036"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY5324213.1"
                     /translation="MESKRLDNAALTAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10803..13196)
                     /gene="malP"
                     /locus_tag="JQR05_002037"
     CDS             complement(10803..13196)
                     /gene="malP"
                     /locus_tag="JQR05_002037"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY5324214.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13808..16513
                     /gene="malT"
                     /locus_tag="JQR05_002038"
     CDS             13808..16513
                     /gene="malT"
                     /locus_tag="JQR05_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY5324215.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELVAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(16556..17572)
                     /gene="rtcA"
                     /locus_tag="JQR05_002039"
     CDS             complement(16556..17572)
                     /gene="rtcA"
                     /locus_tag="JQR05_002039"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY5324216.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITAQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(17576..18829)
                     /locus_tag="JQR05_002040"
     CDS             complement(17576..18829)
                     /locus_tag="JQR05_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY5324217.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTLGTLGTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQD
                     QLETLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVR
                     QPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRG
                     LGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDI
                     DAVMAAQSDLVEVIYTLRQVVCVKG"
     gene            19018..20616
                     /locus_tag="JQR05_002041"
     CDS             19018..20616
                     /locus_tag="JQR05_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY5324218.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(20598..21356)
                     /locus_tag="JQR05_002042"
     CDS             complement(20598..21356)
                     /locus_tag="JQR05_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY5324219.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(21373..22203)
                     /gene="glpG"
                     /locus_tag="JQR05_002043"
     CDS             complement(21373..22203)
                     /gene="glpG"
                     /locus_tag="JQR05_002043"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY5324220.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(22248..22574)
                     /gene="glpE"
                     /locus_tag="JQR05_002044"
     CDS             complement(22248..22574)
                     /gene="glpE"
                     /locus_tag="JQR05_002044"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY5324221.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22764..24269
                     /gene="glpD"
                     /locus_tag="JQR05_002045"
     CDS             22764..24269
                     /gene="glpD"
                     /locus_tag="JQR05_002045"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY5324222.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(24538..24768)
                     /locus_tag="JQR05_002046"
                     /pseudo
     CDS             complement(24538..24768)
                     /locus_tag="JQR05_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         5846 a         6770 c         6299 g         5944 t
ORIGIN      
        1 acattagcca gtccgggtaa ttcactattc gaattatatt ttcgctgcga tataaccttg
       61 agccacatca acattgagtc agattattat tcaaaccaac attcgcacac attttaagta
      121 ttgctgatag aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt
      181 tggcaaatca tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa
      241 tgagaaacaa gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct
      301 tttcccgtga aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg
      361 gctcctcttt tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc
      421 gtcgtgtgag cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt
      481 cctgttaata cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca
      541 aattctggca agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac
      601 tgggctggcg ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc
      661 acgctggtgg acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc
      721 gatgagcaaa tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg
      781 gtggatgcgt ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc
      841 attccctgca tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt
      901 gaaattgatg ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt
      961 ggtcgcggta ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg
     1021 gaactggtgc attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg
     1081 ccttccgaca tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat
     1141 atctacagcc gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc cctcgcccgt
     1201 ctgcgtaatg aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt
     1261 gctgccatct gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact
     1321 gcggtagata aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg
     1381 tacctgatgt tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttgacgtc
     1441 ggctccgtgg cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg
     1501 gactggctga ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca
     1561 ctggtgccgc agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat
     1621 atggcgcgtg cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa
     1681 tcctttgtgc cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt
     1741 acgcttgatg caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc
     1801 ggcgcgcgtc tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc
     1861 ctagcggttt tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg
     1921 ctcaagtaca ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac
     1981 catgtaccac acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt
     2041 ctgcgtgctg gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc
     2101 tcgttgagcg ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg
     2161 gtgataaccc ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt
     2221 ggcctgttta cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac
     2281 accgcagaaa atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag
     2341 ctgttcagtg cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta
     2401 ctgatgaacc ccattgaagc tagcaaaggc gacggtgaaa tgggtaccgg ggcgatgggc
     2461 gtgatggatc agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg
     2521 ctgtatgtac catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg
     2581 atgggcttct ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat
     2641 caggtcgcca gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc
     2701 ctgtttaaca tcgtagttat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa
     2761 ctgctggcaa cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc
     2821 cattaatggc ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag
     2881 cgacccagat aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac
     2941 agctggaaag catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg
     3001 gcagttgtaa aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt
     3061 aaccatactc catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg
     3121 cgtgcggctt tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt
     3181 ctcgcaggga tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat
     3241 tcaaacaaca ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg
     3301 ctgttcaaac tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta
     3361 ccggcaccca ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gagcagcttt
     3421 attgatgacg atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa
     3481 ggaccagaat acatctattg tctgattgaa catcaaagcc ctcaaacaaa ctgatcgcat
     3541 ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa
     3601 cgttgccgat ggtggtgcca ttgttgtttt accacggtat tgaaagcccc tatccctatt
     3661 cgctgtgttg gctggattgt ttcgccgatc ccaaactggc aaggcagctt tatgcctccg
     3721 catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag caccgacgta
     3781 tggcgctgct ggagttaatt caaaaacata ttcatcaacg cgatctgatg gggctggtag
     3841 agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt
     3901 ttaattacat actgcaaact ggcgacgctg tacgttttaa cgattttatc gacggcgttg
     3961 ccgaacgttc accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg
     4021 agggggaaca atccaaagcc ctgcatatag ccaaaataat gcttgaatcc ggagtccctc
     4081 ttgcagacat catgcgattt accgggctgt cagaagaaga gttggctgcg gcgagtcagt
     4141 agatactcta ccctgcttaa gtgctacttt gtgctatctt gcatgtaaca caaagtagca
     4201 cttagggaac tgatatgcag gcagtgaaat tacggcaaca aggaggagcc atgatcgtta
     4261 ctatcccacg agatcttgcc atcgatcttg gctggtcacc gggcacagag cttaccgtgg
     4321 agaaaaaagg cgatagcgtt aatttgcgcg ccacagagca caaaccgcgt gggcgtctta
     4381 ccgtcgcgca gttgttgagc caaatcgatg aaagcgaaat tacggagcta aatcagtcca
     4441 cagaaggatg ggctgaaggg aaaaaaggta atgaagcatg gtaaaggcac ggacgccaca
     4501 tcgtggtgag atctggtatt ttaaccctga tccggttgcc gggcatgaac ttcaggggcc
     4561 acattattgc attgtggtaa cggacaaaaa actcaacaat gttttaaaag ttgctatgtg
     4621 ctgcccgatt tcaacagggg caaatgcagc acgttccaca ggggtgacgg tgaacgtcct
     4681 cccccgtgat acgcaaaccg gtaacctgca tggcgttgta ctttgtcacc agctaaaagc
     4741 cgtcgatctt attgcccgtg gcgctaaatt tcataccgtt gccgatgaaa aattgattag
     4801 tgaagttatc agtaaactgg tgaatttaat cgacccacaa taattagctc tcacttacca
     4861 ccaacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga tgcgaaataa
     4921 acggcgcatg ggccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca
     4981 gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg
     5041 gcatggacac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagcccgc
     5101 cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
     5161 catcctggcg cgccgtttca gtccccatgg tttgtaacgc caggaaccgc tccactgtac
     5221 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
     5281 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
     5341 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
     5401 aaatggcttt atcaggtgcc tgttgcagca cggcttcggc catatcagca agtgacagcg
     5461 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
     5521 agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
     5581 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
     5641 gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
     5701 gccgaatgcc actggcgtta ggtcattggg ggatttgttc ggtctgttca cgcaccaccc
     5761 gcacagataa aacgttatgc ccacaatgtg gattacccgc cacacactcc catcttccct
     5821 gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt gccgactatg
     5881 cgccgccgtt aagtccgctt atccaccagc ttaaattttc ccggcgcagt gaaatcgcca
     5941 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
     6001 aattgccgga tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
     6061 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcactgc caatgggata
     6121 gcgaagccgt cacacgtaca cgggccactg cgacccagca ttttctcagt gcccggctgc
     6181 gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg
     6241 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
     6301 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
     6361 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
     6421 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca
     6481 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
     6541 tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
     6601 tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgactt tgttaccgac
     6661 cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca aaatgcgtaa agtggcagac
     6721 gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
     6781 gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta cgccattctg
     6841 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
     6901 gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
     6961 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
     7021 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggca cagcattgct
     7081 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
     7141 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc
     7201 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
     7261 gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc
     7321 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
     7381 ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
     7441 gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa
     7501 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
     7561 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
     7621 tttggtaaaa agcacatcca gtgggcggtg gtgttaaccg gttttaccgt tggttttgcc
     7681 ctgttctatg aagtgggctt cgtgctgatg ctgccgctgg tgtttaccat cgcggcttcc
     7741 gcgaatattc cattgctgta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac
     7801 ggcttcctgc caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgatatg
     7861 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
     7921 gtttacgctc gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg
     7981 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg
     8041 ccggttgtgc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
     8101 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg caacgctgat tgccgtgctg
     8161 attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg
     8221 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
     8281 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
     8341 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
     8401 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
     8461 acgggtgtta gcccagagct gatggttatt gcggtgggtt ccggtagtgt gattttctct
     8521 catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag
     8581 accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
     8641 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
     8701 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
     8761 ttgttcacgc catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag
     8821 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
     8881 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg
     8941 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
     9001 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
     9061 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
     9121 ctctcccagt aaccacgcag cgttggcagg tcatgtgtcg ccgcaaccgc catcgactgc
     9181 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
     9241 ttgtaagagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
     9301 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
     9361 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
     9421 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
     9481 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc
     9541 attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
     9601 gatgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg
     9661 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
     9721 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca
     9781 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttcttata catctctggc
     9841 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
     9901 aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggcc
     9961 atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    10021 agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt
    10081 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg
    10141 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg
    10201 gcgctctccg gatttgccgg atagagcgca tgaatcgggt tcaggccaat gaacgacccg
    10261 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    10321 tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc
    10381 agcaaagcct gcggttcgta acaacgtttc ggggcgacaa tcacccggca atgcgcgcgc
    10441 tggtcgtcct gggtgagtgt tagcgtgtga taaccttccg gcagcttcgt cggtagattg
    10501 aacgctttgc cccccgttac atggcctttg tactgcgttc cttcttcggt ggtcagctgc
    10561 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    10621 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    10681 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    10741 ttggggctaa tccccgccgt cagcgcggca ttatccagac gtttgctttc catcgagctt
    10801 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    10861 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    10921 tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga
    10981 tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    11041 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    11101 ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta
    11161 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca
    11221 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    11281 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    11341 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga
    11401 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag
    11461 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg
    11521 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    11581 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    11641 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    11701 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    11761 ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt
    11821 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    11881 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    11941 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc
    12001 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    12061 aagcgagtat taatttcgtt aataatctgc atatggcgcg gtagtaagcc tttcaccagt
    12121 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    12181 ttgctggtga tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    12241 agcagttctg gaatcgcgat agttggatgg gtatcgttca gctgaataac ttcgtaatcc
    12301 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    12361 caggcacact ggaagtattg ctgcatcagg cgcagctttt taccggcagt atggttgtcg
    12421 tttggataga gaactttggt cagtttttcc gcattgatgc cttgctgttc ggcgcgcagg
    12481 aaatcaccgt cgttaaattt agttagatca aacggatgcg catgcgtcgc ctgccacaga
    12541 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    12601 gtaatggtaa actccggctc ccagcgtccg tctttcatca ctttaccgcc aatccctacc
    12661 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc
    12721 ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc
    12781 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc
    12841 agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtcagtc
    12901 aggttgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    12961 aggttgttgc ccgtcagacg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    13021 tgattcgcca ccggtttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    13081 actgccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    13141 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    13201 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    13261 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    13321 tgatctcgac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgccatac
    13381 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    13441 aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatccagtgt
    13501 gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag
    13561 gaatgaagaa gaaaccctta cttaaatgaa tctgatgaac ataagggaaa ccagtattca
    13621 cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg tgacacagtg
    13681 caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt
    13741 ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg
    13801 attaactatg ctgattccgt caaaactaag tcgtccggtt cgactcgacc ataccgtggt
    13861 tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac
    13921 gagtcctgcg ggctacggaa agaccaccct catttcccag tgggcggcag gcaaaaacga
    13981 tatcggctgg tactcgctgg atgaaggtga taaccagcaa gagcgtttcg ccagctatct
    14041 cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga cgatggcgca
    14101 aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga
    14161 atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta atccagtgat
    14221 ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt
    14281 gtcacgcaac cttccgcaac tgggcattgc caatctgcgt gttcgtgacc aactgctgga
    14341 aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg
    14401 tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt tgcgatgacg tttccggttg
    14461 ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact cagcccataa
    14521 gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga
    14581 ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg
    14641 ctcaatgaac gatgccctca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgtct
    14701 cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgagtggtt
    14761 ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg aactggtggc
    14821 ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg gatttcccag
    14881 cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct
    14941 taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa
    15001 ggccctgccg tgggacagtt tgctggaaaa tccgcagtta gtgttattgc aggcgtggct
    15061 aatgcaaagc caacatcgct atggcgaagt taacaccctg ctagcccgtg ctgaacatga
    15121 aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt
    15181 ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga
    15241 gctgccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct
    15301 gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc
    15361 acgccagcac gacgtctggc actacgcctt atggagttta attcagcaaa gtgaaattct
    15421 gtttgcccaa gggttcctgc aaaccgcgtg ggaaacgcag gaaaaagcgt tccagctgat
    15481 caacgagcag catctggaac agctgccaat gcatgagttt ctggtgcgca tccgagcaca
    15541 actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga
    15601 agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg
    15661 ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ttgaaccgtc tggaaaacct
    15721 gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat
    15781 ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa
    15841 accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg cccgcgcaca
    15901 aatcttgctg ggcgagtttg aacctgcgga aattgttctc gaagaactca atgaaaatgc
    15961 ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctta atcaactgta
    16021 ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat taaaactggc
    16081 gaatcgcacc ggatttatca gtcattttgt catcgaaggc gaagcgatgg cgcaacaact
    16141 gcgccagctg attcaactta atacgctgcc ggaactggaa cagcatcgcg cgcagcgtat
    16201 tctgcgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt
    16261 tgaacgtctg ctaaatcatc cggaagtgcc agaactaatc cgcaccagcc cgttgaccca
    16321 acgtgaatgg caggtcctgg ggctgatcta ctccggttac agcaatgaac aaattgcggg
    16381 cgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact
    16441 cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga agatgatggg
    16501 ttatggggtg tgagtttagc cggataatgc attggatccg gtttacatct ttacatcatt
    16561 caatactcac ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac
    16621 gctccaccac cgcgatattg gtcagcagat ggcatgaagg atgggcgacc gtaaattctc
    16681 ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc gagatattcc cccaccgccg
    16741 ctgggcttgc caggtagcgt ttcacctctt tcactaactg cgcggctacc acctcggcac
    16801 tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat attttcactt tcgacttcca
    16861 gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc tgttcatgca
    16921 gggaaaaact ccccgccagt gtagcgattt cacgctcagc aacatggcgc ggcacaccag
    16981 ctaatagaac ttctccacgc atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt
    17041 taaacgatgc cacgggcgag acttccgttg ccaccacacc gcctccggca ggataaaaac
    17101 cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc ggctccagca
    17161 cccggcggat aaaatccgca ggcggcgccg acgggttatc ggtgccgccg ctcacttcaa
    17221 cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc accagcgtac
    17281 aacttccggc gctaccgata gcaaagcggt aatcgccgcc gcgcacggtg ccaggccgga
    17341 agaccagacg ctgcgatccc agctccgcgc cttccaccgt tgccctacaa atttccgcag
    17401 ccgcttttac cgcggtcaga tgctggcgca gcagccccgg tttcgcccgt ccggctcgaa
    17461 tgccagtgat ggtaaatggc tgggcggtta tcatcgacag gctcagcgcc gagcgcagga
    17521 tctgcccgcc gccttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc
    17581 cttttacgca caccacctga cgcagggtgt agataacttc caccagatcg ctttgtgccg
    17641 ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt
    17701 tacggcattc cacatgcgcg gtggcacgaa tttggtcttc cacgctgaac agttttttcg
    17761 ctttagttcg gctcattacc cgcccggcac cgtggctgca cgaacagaac gactcttcat
    17821 ttcccagccc acggacgata aagctttttg ctcccatcga accgggaata attccatatt
    17881 gaccagcacg cgcagacacc gcacctttac gcgtcacgta gatctcttca ccaaagtgct
    17941 gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgtggct
    18001 gtctgaccgt tttctgcgta atgctctgca acgccgttac cacattttcc atcatcgcat
    18061 cgcggttaag gctggcgaaa agctgcgccc aggccacggc tcttaggtaa tcatcaaagt
    18121 attccgtacc ttccataaag tacgccagat cacgcgacgg caacgtctca agttggtcct
    18181 gcatctcttt ttgcgccaga tcgataaagt aagtcccgat ggcgttacca attccgcgtg
    18241 aaccggagtg cagcataatc cacacctgct ccgactcatc aaggcagatt tcaataaagt
    18301 ggttacccgt tcccagcgtt cccagcgttc ccagcgttcc cagcgttccc aggtgtttat
    18361 agttgttagt gttaaggaaa cgcggatatt tttgcgttaa ccactgataa ccggcctcca
    18421 gctcagccca tttcgcatcg acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt
    18481 tacaacgccc ggtagtacgc ccgtgtggta cggccgtttc aatcgcttga cgcagctccg
    18541 ccaggttttc aggcaggtct gccgccgtta acgcggtacg cagcgcgttc attccacagc
    18601 caatatccac gcccaccgcc gccggaataa tcgccccttt ggtcgggatc acgctaccaa
    18661 tggtggaacc tttacccagg tgtacatcag gcattaccgc aatatgtttg aaaataaacg
    18721 gcatcttcgc cgtattaata agttgctgac gcgcatcggc ttctaccggc acgcctttgg
    18781 tccacatttt taccggggca ttttcagtgg tcagtaattc gtaattcatt ttgtttttct
    18841 cttttcgttg tttgcggtcc tgataattgc aaccgtcgtg ccagaaaatt aaataagcag
    18901 attaattttt taatttattg tttctaaaaa gattatatct ttatgtccgt aaacggagat
    18961 ttcccgcaaa gccgatttac cgataatgaa atatcgcctt ttataaggat atctaagatg
    19021 cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg attatgccgg acgcggcagt
    19081 cagcgctggt caaaatggcg tccgtcactc tgtatatgcc agcaagaatc gttggtcatc
    19141 gatcgactgg aattattgca cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc
    19201 gatatcgcca gcgtttcgcc agaaacagaa gtggtgggcg ttgagattga actgcataac
    19261 ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt
    19321 cagccagaaa aagaagacta tttaattcac atcaccaccg gcacccacgt cgcgcagatt
    19381 tgctggtttc tgctggcgga agcgcgttat ctacccgccc ggttgataca atcttcaact
    19441 ccgcgcaaaa aagaacagcc gcgcggccca ggtgaagtga cgattatcga tctcgattta
    19501 agccgttata acgccatcgc cagccgcttt gccgaggaac ggcagcaaac gctcgatttc
    19561 ctcaaatccg gtattgccac gcgtaacccc cacttcaacc gcatgattga gcagatcgaa
    19621 aaagtggcga ttaaatcccg cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag
    19681 tcatttctgg cgcgacgcat cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg
    19741 tttgtggaag tgaactgcgc caccctgcgc ggcgataccg cgatgtcgac gctgtttggt
    19801 catgtgaaag gcgcgtttac cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc
    19861 aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga acaggcaatg
    19921 ctgctgaaag ctattgaaga gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc
    19981 agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt tgccgaaggc
    20041 aaattccgcg aagatctgta cgcgcggatc aatctctgga ccttcaccct gccgggtcta
    20101 cgccagcgcc aggaagatat tgaaccgaac ctggattatg aagtggagcg ccacgccaca
    20161 ctcactggtg acagcgtgcg ttttaacacc gaagcgcggc gtgcctggct ggcctttgca
    20221 acatctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg
    20281 atggccacct ttgccactag cggacgcatc actctggacg tggtggaaga tgagataaac
    20341 cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca
    20401 gaggcagaaa acatcgatct cttcgaccgt atgcaactgg aacacgttat cgctatctgc
    20461 cgccaggcaa agtcgctttc tgctgccgga cgacagcttt ttgatgtttc gcgccagggc
    20521 aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg
    20581 tgggaagccg tgcaggatca gcacagctcc agttgaatat ggtggtccgt cagcacctgc
    20641 atcacgctta ctggcggcgg ggcgtcggtg tagacggcat caaccatgct gatgctcccc
    20701 atattgacca tcgcattacg gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc
    20761 gagttctcaa taatggcgcg cttggtacga acttcgtggt aatcgaactc cagcagcgag
    20821 ccgtcgctgt cgatgccgct gatccccaga atgccgaaat caaggcggaa ctgggagata
    20881 aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tacgtaattc gccaccggcg
    20941 agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacgttgag attgttggtg
    21001 acaatgcgca aattgctgtg attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg
    21061 atatcgataa acagcgtcga gccattgggg atttgctccg ccactttgcg ggcgatgcgc
    21121 tctttttctt cggtctgggt ggctttacga tcgtgccacg gcgtgttaac cgaactggaa
    21181 ggcagcgccg cgccgccatg atggcgcagg atcaggtttt gctctgccag ctcgttgagg
    21241 tcgcggcgaa tagtctgcgg gctgacggag aagtgctcca ccagttcctc ggtactgaca
    21301 taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg tttcatttat
    21361 aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa
    21421 acccacggct aatccggcga tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa
    21481 ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc
    21541 actttgcgga tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat acaccacgcc
    21601 agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc ttaacagggc
    21661 gctgataagc gtaatgacaa ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc
    21721 gagataccac caccaaagca ggttaaagag gatatgcatc agcgagaagt gcattaacgc
    21781 gtgggtgaag taacgccaga actcaaattt cagtgttgga tcgaatggcc aggccagcca
    21841 taacatcact tcctgatcgc cgagaatttg catggcaata aacaccacca cgcaggcgat
    21901 catcatcacc caggtcaccg gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg
    21961 gcgataatgc aggccactgc cggtatggcc tgccagccag ctggccgcca gataacgcgg
    22021 atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg cctgggactc
    22081 atccgccagc cagacatcgc tttggttatg ttgttgaatc gtgaggataa caccctgcgt
    22141 cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg taatcatcaa
    22201 catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta cgcgccgtac
    22261 gccacctctg cgggaaactg tcgctgccag gcttcaaagc cgccgtcaat gctatagacc
    22321 acatcgtagc cctgttgcag cagatactgc gccgcccctt tgctgctatt gccgtgataa
    22381 cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg
    22441 ttggttaaat ggaaagcctg caccgcatgc cccatagcga aactctgtgg atcgcgaata
    22501 tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat
    22561 tcgaactgat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt accgcacagt
    22621 ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa aatgtttttt
    22681 caatgttacc caaagcgcga ttctttgcta atatgttcga taacgaacat ttatgagctt
    22741 taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca
    22801 tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg
    22861 aggcgcagga tctcgcttgc gcgacctctt ccgccagttc aaaactcatt cacggtggcc
    22921 tgcgttacct tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag
    22981 tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc
    23041 cgcatctgcg cccggcgtgg atgattcgca ttggtctgtt tatgtacgat catctgggta
    23101 aacgcaccag cctgccggga tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac
    23161 cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac
    23221 tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca
    23281 cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc gataccggca
    23341 aaaaatatag ctggcaagcg cgcggcctag ttaacgccac cggcccgtgg gtgaaacagt
    23401 tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc
    23461 atattgtggt gccgcgcgtg catacccaaa agcaggccta cattctgcaa aacgaagata
    23521 aacgcattgt gttcgtgatc ccgtggatgg acgagttttc catcatcggc accaccgatg
    23581 tggaatacaa aggtgatccg aaagcagtga agattgaaga gagtgaaatc aattacctgc
    23641 tgaaagtgta taacgcgcac tttaaaaagc agctaagccg tgacgatatc gtctggacct
    23701 actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaagcc attacccgtg
    23761 attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg
    23821 gtaagctgac cacctaccga aaactggcag aacatgcgct ggaaaaactg acgccgtatt
    23881 atcagggtat tggcccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag
    23941 gcgaccgcga cgattacgcc gctcgcctgc gccgccgtta cccgttcctg actgaatcgc
    24001 tggcacgtca ctacgcgcgc acttacggca gcaacagcga gctgctgctc ggcaatgcgg
    24061 gaacggtaag cgatctcggg gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat
    24121 acctggtgga tcacgaatgg gtccgccgca ccgacgatgc cctgtggcgt cgcacaaaac
    24181 aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag tcagtggctg gtggaatata
    24241 cgcagcagaa gttgtcgctg gcgtcgtaaa ttaacgtaag gtgatcaggt cagatttcaa
    24301 tctggcctga gactgatgac aaacacaaaa ctgcctgatg cgctacgctt atcaggccta
    24361 cgtggtttat gcaatatatt gaatttgcat ggtcttgtag gccagataag acgttcacgt
    24421 cgcatccggc atgaacaaag cgcactttgt caaaaatcta acctactttt taattcaggg
    24481 aattaccgca aagcccacgc catcataccc aacgtaacaa aactcaggca cgttccccac
    24541 tcgccccgag aaaaatagca ttaatgcgcc cagcgtcagc ataaaaatat tgagcagaag
    24601 gtaaattgtt ggcgtaaaga gtaaattgca aacctcagga atagcggcaa gcccagcccc
    24661 cagcatggca ataaccgctg caaaaaattg cgtcagtatt gggatgccga aaaaagtcat
    24721 taccagcgag gtcaaaatcc attttctatt ttgcattatt ctttccattc tttctgaatg
    24781 gcgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
    24841 aaaaaaacgg gaccctttg
//
LOCUS       DADKXM010000052        24314 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.052, whole genome shotgun sequence.
ACCESSION   DADKXM010000052 DADKXM010000000
VERSION     DADKXM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.052"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JQR05_002047"
     CDS             <1..848
                     /locus_tag="JQR05_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5324223.1"
                     /translation="SRFAFSDDPKWESGHHVHDNETELIYVKKGVARFTIDSSLYVAH
                     ADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGK
                     EVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLI
                     KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(891..2042)
                     /locus_tag="JQR05_002048"
     CDS             complement(891..2042)
                     /locus_tag="JQR05_002048"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY5324224.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYSRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2056..3651)
                     /locus_tag="JQR05_002049"
     CDS             complement(2056..3651)
                     /locus_tag="JQR05_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY5324225.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLIIALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYAIFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFRGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3797..4555)
                     /gene="aroD"
                     /locus_tag="JQR05_002050"
     CDS             complement(3797..4555)
                     /gene="aroD"
                     /locus_tag="JQR05_002050"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY5324226.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4586..5452)
                     /gene="ydiB"
                     /locus_tag="JQR05_002051"
     CDS             complement(4586..5452)
                     /gene="ydiB"
                     /locus_tag="JQR05_002051"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY5324227.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(5464..6729)
                     /locus_tag="JQR05_002052"
     CDS             complement(5464..6729)
                     /locus_tag="JQR05_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324228.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6956..8170)
                     /locus_tag="JQR05_002053"
     CDS             complement(6956..8170)
                     /locus_tag="JQR05_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324229.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(8270..8626)
                     /locus_tag="JQR05_002054"
     CDS             complement(8270..8626)
                     /locus_tag="JQR05_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY5324230.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8762..8869)
                     /gene="rprA"
                     /locus_tag="JQR05_002055"
     ncRNA           complement(8762..8869)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JQR05_002055"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(9055..10167)
                     /gene="ydiK"
                     /locus_tag="JQR05_002056"
     CDS             complement(9055..10167)
                     /gene="ydiK"
                     /locus_tag="JQR05_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY5324231.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10556..13612
                     /locus_tag="JQR05_002057"
     CDS             10556..13612
                     /locus_tag="JQR05_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY5324232.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13609..14019
                     /gene="menI"
                     /locus_tag="JQR05_002058"
     CDS             13609..14019
                     /gene="menI"
                     /locus_tag="JQR05_002058"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY5324233.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            14119..14307
                     /locus_tag="JQR05_002059"
     CDS             14119..14307
                     /locus_tag="JQR05_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324234.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14855..15223
                     /gene="sufA"
                     /locus_tag="JQR05_002060"
     CDS             14855..15223
                     /gene="sufA"
                     /locus_tag="JQR05_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY5324235.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            15232..16719
                     /gene="sufB"
                     /locus_tag="JQR05_002061"
     CDS             15232..16719
                     /gene="sufB"
                     /locus_tag="JQR05_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY5324236.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKSVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16729..17475
                     /gene="sufC"
                     /locus_tag="JQR05_002062"
     CDS             16729..17475
                     /gene="sufC"
                     /locus_tag="JQR05_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY5324237.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            17450..18721
                     /gene="sufD"
                     /locus_tag="JQR05_002063"
     CDS             17450..18721
                     /gene="sufD"
                     /locus_tag="JQR05_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY5324238.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTESLRDEGLKQQVLARIGQRLPGGAR"
     gene            18718..19938
                     /gene="sufS"
                     /locus_tag="JQR05_002064"
     CDS             18718..19938
                     /gene="sufS"
                     /locus_tag="JQR05_002064"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY5324239.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19951..20367
                     /gene="sufE"
                     /locus_tag="JQR05_002065"
     CDS             19951..20367
                     /gene="sufE"
                     /locus_tag="JQR05_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY5324240.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20516..21520
                     /gene="ldtE"
                     /locus_tag="JQR05_002066"
     CDS             20516..21520
                     /gene="ldtE"
                     /locus_tag="JQR05_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY5324241.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21584..21820)
                     /locus_tag="JQR05_002067"
     CDS             complement(21584..21820)
                     /locus_tag="JQR05_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY5324242.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(22131..23543)
                     /gene="pykF"
                     /locus_tag="JQR05_002068"
     CDS             complement(22131..23543)
                     /gene="pykF"
                     /locus_tag="JQR05_002068"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY5324243.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            24100..24309
                     /gene="fumD"
                     /locus_tag="JQR05_002069"
     CDS             24100..24309
                     /gene="fumD"
                     /locus_tag="JQR05_002069"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY5324244.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6352 a         5831 c         6115 g         6016 t
ORIGIN      
        1 tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac gttcatgaca
       61 atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc gattcttcgt
      121 tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg catgcggtgg
      181 cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc
      241 agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt gccgcggggc
      301 agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg ccgcaaagta
      361 aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca attctttact
      421 acgaaaactt taaaaatgct tatcgttcgg agcaggggta tattaaaaaa gatgttctga
      481 taaaagatat tcttttctat ctgaataata attatcgcga aaaaatcact ttagaacagt
      541 tatcgaaaaa atttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc
      601 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcaaaa tggtcactca
      661 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      721 actttgccaa actgtttttg cgccatgtcg gttgttcgcc cagcgattac cgtaggcaat
      781 ttaaaaattg ttttgcggaa caagaaatct tatctgaatt tcctcaaccg gtaagtcttg
      841 ccggataaat ctggcggcgc actttgttaa aaagctgcgc cgcctgcaga ttatttgttc
      901 tgatagtctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
      961 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
     1021 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcacag
     1081 tacagttttg ccagcgccgc gctggtgcgc agtgattgat gctgatcggc ttgccatgcc
     1141 actttcagca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
     1201 tggatcatct ggttatgccc aatgggttta ccaaaagcga tacgctggtt ggcatagcga
     1261 gcggcatctt caaaggcaca ctcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
     1321 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
     1381 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
     1441 attttatgca gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg
     1501 aaggcttttt tgggatcttt cggttgcgga tcgcgcgcca acaccagcat atacgggtac
     1561 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
     1621 gtgtaagtgg tagtggcact gttgttgtct gagcctgcgc ctggctccgt caacgccagg
     1681 gcataggcgg gatcacccgt ttccagtgtg ctttctgccg ttttacgtag ctgctctgca
     1741 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taatcaaaaa tgccggagca
     1801 ccgcattttg acacttccat cagcgccagc atctgggtaa cgtaatccgc agggataccg
     1861 ccgaactctt ccggcacacc aagcatggaa ataccgttat ccgccagtgc ccgcataaac
     1921 tcacgcgggt atgtcccgtt ttgatcgcag gtgcgggaat actcttccgg aaagttagtc
     1981 gtaatcaact cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatct
     2041 atcgtgatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     2101 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga aatccatttt
     2161 gtcgagaata tctttttgta aatcgacgcc aggagcgatt tcgattaaat gcaggccgtc
     2221 ttctttcagc gtgaatacag cacgttcggt gatgtaacga acatccagtc cccgttcgag
     2281 cgcgattttt ccgcggaaag taatttccgg tagttcccga ataaatttct tcacccgtcc
     2341 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
     2401 cgtgccgcag aaaataattt tcttcgaggt ggcactgata tcaataaatc caccggtacc
     2461 catgattttg ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2521 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2581 cagaatggcg cgggtattca cgttcgcgcc aaaggcgata ccctgtgaag taattccgcc
     2641 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgagcgac
     2701 caggccaatg ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc
     2761 gaataatgcg cgccgcgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtact
     2821 gtcatcaagg gtgaagtcac cagaaataaa gcggttaacc ggtgcaccgc catacagttg
     2881 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2941 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     3001 attgtgcact gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaaat
     3061 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc
     3121 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     3181 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgtcccga tgccaatatc
     3241 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     3301 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     3361 tccccagtga ccgcataatg cccatttcac cagaccttct tgcgccagcg gactaatacc
     3421 gcggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggcgtttg
     3481 agtctgttta tatttatcag caagagcaat aattaacgtg gtggcttcca gaataccgcc
     3541 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3601 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3661 ctgattcagg tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa
     3721 atatttttat aaaatacggt taaaaatact ttttttattt cggcggggga gggtatttcc
     3781 gccgaaatat tattgcttat gcctgatgta aaatagttaa taccgtgcgc aaatcattta
     3841 ccgagatttg ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc gccgagccaa
     3901 atacttcacc agccagacga gaaattacgc cagtttttgc catcgacatc gtgatgattg
     3961 gacgatcggc atactgctcc tgcatctcca gggtcgcggc aagcaacgtc agcacatcgc
     4021 tggtactttg cggcatcagc gcaatcttag gaatatcggc gtcgaaggac tgcattttgc
     4081 gcagacgggc aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga
     4141 caactttcac atcatgcgcg tgggcgtagg cgacggtttc tttgacctga tcatcgccgg
     4201 taaataactc cagatcgatc atatcaacca ggccgctgtc gatggctgca cgattgagag
     4261 caatataagc ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa
     4321 acagcagcgg tttttctggc atggtttcac ggagaatttt tgccgccgcc atgacagact
     4381 ccacattgga gaggtcggca aagtggtcca cacgccattc cagaatatca aagtccgctt
     4441 cacgataggc gagagcttcg gatttcacgc gggcgatatc tttcgccatc agcgagacga
     4501 tgattttagg tgcgcccgca ccaatgacga gatcttttac agttacggtt ttcatttttt
     4561 accctttctg cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt
     4621 ccagagggaa atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca
     4681 tgccgtatcc atcaatcgtt ttgcaaccag cttgttgtgc ctgctgcaat aacttcgtca
     4741 tatgcgggtt atacacgcat tcagtgacca gcagtcccgg atgtaacaga ctgatatcat
     4801 taaccaatga ttcattctca aggggtttca tacctacttt tgtgccattg gttaaaatgt
     4861 cggcggaagc cagggcttca gcaaaggctt gctgatcggc gagatcggta accgtgacga
     4921 cacaatcggt gttttcgtta acccgctgcg cgaaggcgag ggctttatcg aagaactcat
     4981 cccgacggtt aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaatcg
     5041 ccgttgaggc acctccggcc cctaacagca ccatcgtttt gcctttgata tcaaaaccgc
     5101 tctctttaat ggcgcgaata tggcccgtgc cgtcggtgtt atagccacgc agatagccat
     5161 catcattaac gatggtgttg atggcaccca ccagtttggc tgctggcgtt aattcatcaa
     5221 catattcaca cgccagttgt ttgttcggca tcgacacacc agttccgcgc attttgaggg
     5281 cttttaatcc ttcaattgct ccgggaaagc tatcgttatc cacttcgaag gccatatagg
     5341 taaatggcaa tcccgctttt tctaaggctt tattctgcat ttcaggcgat aaactgtggc
     5401 ggataggata agccatcaat ccaatcaatt cgtattttgc ggtaacatcc ataattaact
     5461 cctttaacct ctatgcttaa ttgtgtttaa ccgggtgcta aatttgcgtt cgccaaaccg
     5521 cacatcattc tcaggaataa taaaaacgcg gtaatagcgg ctaaaaacaa taattgcggt
     5581 aatcagggcc agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt
     5641 cgacagataa ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata
     5701 aatactggtg actttggctt tgcttttggg gaaaaactct gacatgaccg aaacaccgag
     5761 ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag gcgccggcat tacacaccag
     5821 tggagaaggg tacaggtaga taatggcagc tgttattgtt gccagcgcag agttaaatac
     5881 attagcccag atgggccgga ccattttttt cagtagtgcg gcaaaaataa agacacagac
     5941 caacgagccc atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc
     6001 aaaagccatc gcatatttgg gcatccacac cacaatcaca taaaaggtcg agaatgcggc
     6061 tacaccgaac agtaccgatg aaacaccttc cagccagact aacggtttgc tgttcatttg
     6121 cggtaattca ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag
     6181 catcagcgtg atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat
     6241 attattgagc aacatatagc tcaccagcat tgggtagaac atttgcccaa atgacaccat
     6301 cgctttaacc agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg
     6361 gtagccaccc gtatccagcg ctgagttagc gatacctacg cacactgcca gaccgtaggc
     6421 gagagttaaa ttcgggcaag cgggaatacc aaagaagaat agcagataca ttattactgc
     6481 cattaatatc accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact
     6541 gatcaatcgc cccaaaccga taccggaaat taaataggca atgcccgcgt tgtcagtgga
     6601 aaacttttct gccagagatg tcatattttg ggcaagcgta ataacactaa tgccgtgcag
     6661 gaaatagctg aagtaaatac aaagaacagc caggataaat ggcgtgctga aagccttatt
     6721 ttgagacatt ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc
     6781 cgacggttta cataattact atactgaaga tgattttccc tgggtacatg gcataaagtt
     6841 tatgtttcca ggaatattgc atgtcatagt ttgtttttga tcgcgatttt ccttagttag
     6901 cgcagaaaga gttatttatc cgtaagatct tgcatgacct gaaaagaata ttgcattacc
     6961 cacccggagc gacattttcc tttagcgagt gatgccgcgt ttttttgcgg ctgcgtaagc
     7021 cgataaacag tgccagtaaa aaaccgatgg cagcgatggc ggtatcgaac cacataatat
     7081 cggcaatact tctttgggac agatgagccg taatcaacgg aatagtaaag gtcgcaatac
     7141 tgcccgcact gtaatagatc cctgtagctt tacctttagc gtaagggaaa cgttcagcca
     7201 ttaacgtcag gccaatttgc acaacacctc cagcagaggt aaaaccaatg acaaaagcaa
     7261 atattatcac cacataaaat gtgggatgca ggcagacggt aaacagagca ataaatgaga
     7321 taaaggtgta cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcgg
     7381 taataaatac acaaagcagc gaacccacgg tgtagatact gagtagtttg atcgacatag
     7441 tgtatgacat gcctgcaaca aattgtccgt actgtgccag ccactggcta accagataaa
     7501 acgttgccat tgagatatag ccatataagg tataactggc taaatcgata attgaacagc
     7561 gatgttccgt agagctggtg gttttcttta tgacaggtaa gcgacgaccc ggatggggtg
     7621 ggaacgtaca acggtataaa aacagagcgt taataaacat aatgcctgca gcaatcataa
     7681 aggaccaacc gaaccacagt tcagcccaca ctaacaggct aatgattagc ggtaataaaa
     7741 attgtccgct ggaaacaaat gctttaatta aaatattggc tgtcccaggt gagcgtggaa
     7801 aagcttccat caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca
     7861 gaaagccaaa aacataagcg ataatgatgt tattggtctg caggatgcca aaaaagaagg
     7921 ccatatagca gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata
     7981 atcctgcaaa aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga
     8041 cacccgcggc attagtctgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa
     8101 tgacgcccat accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg
     8161 gatttttcat aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag
     8221 tatccattca ttattgttgt aacaacgaca attcctttct gtggaaaaat taacaaagcc
     8281 gctccagatc gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa
     8341 aattgccatc agggtagact tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt
     8401 tgttgccgat gcggttattt attttctcta tgatggcatg aagatgtttt tttcgttgct
     8461 gacgcatagc caacagttgc tcgactacac aatcaggaat ggcgcatttg ccattttccc
     8521 actgacgcca gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag
     8581 tcatggcaaa aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc
     8641 atgaaataaa caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc
     8701 acagtatctt gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat
     8761 gttaaaaaaa agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc
     8821 actcagggga tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt
     8881 aggcatacgg actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa
     8941 tttgcgacag gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc
     9001 aaatatcaat tagcggtgat taacaatcag gcggcagagc cgcctgatga gaaattactt
     9061 attcggtttc tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg
     9121 tgggacttct tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc
     9181 aataaacaga ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag
     9241 gatcagcggt aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt
     9301 gccaaccaca ccgctccaca ctaacaatac cgttccccag gtggtatcgc cggtccagta
     9361 gagccagata atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggagag
     9421 gatcattaac accgttagca acgttgcata aggtacgccg gatacggcga ggccgatacc
     9481 gccaagcact gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc
     9541 ctgtgccgcc agcagcacgg cggcatctcc gcgaacgcct gccagacggg ttgcaaaatg
     9601 gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat
     9661 cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt
     9721 ggtggtgcca atataagggc ggactttcgc catgatcgcc gtacccccca tatccagcaa
     9781 gttgtgccag cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag
     9841 atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt agcgggccgc tgccgtcgac
     9901 gatactgtta accagcaaag caatagggat gataaacacc atcactaata acagcgtcat
     9961 caccagaacg gcgagagaac ggcggccaaa catgatcttt tgcaaacgta acaataccgg
    10021 ccaggtggcg ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat
    10081 ccacagacat gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac
    10141 atccctgggc tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg
    10201 tcgacgcgac gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt
    10261 taacaggccg attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca
    10321 actcaggcaa cacgcaaacg atttactcgt cgtatttcaa gttgcatgtg ctgcggctac
    10381 gttcgttcac cccagtcact tactattgta agctcctgga gttcactcgc ttgccgcctt
    10441 cctgcaactc gaaatccata gagtaaataa acatcacctt tcagtgcgct attgcagccg
    10501 cctgaaagaa tagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat
    10561 tccacagatt tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct
    10621 ggagcaacaa ggttttaccg gcgatgcggc gacaagttat gccgatcgtc tgacaatgtc
    10681 gaccgacaac agtatttacc aacttctccc cgatgcggtg gtatttccgc gttcaaccgc
    10741 agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac
    10801 cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc aaccagggga ttattgttga
    10861 tatgtcccgc catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt
    10921 tgaggccggg gtgataaaag accaactcaa tcagtacctg aaaccgttcg gctacttttt
    10981 tgcgccggaa ctttcgacca gcaaccgggc aacgctcggt gggatgatca ataccgatgc
    11041 atccggtcag ggatcgctgg tctatggcaa aacgtcagat cacgtacttg gcgtacgcgc
    11101 ggtgttgttg gggggcgata ttctcgatac gcaaccttta cccgtcgagc tggcggaaac
    11161 gctgggtaaa tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg
    11221 tcagcaacgc cagttaatca tcgacaactt ccccaaactt aaccgctttc ttaccggtta
    11281 cgatctgcgt catgtcttta acgatgagat gaccgagttc gacctgacgc gcattctgac
    11341 gggttcagaa gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcgcttgcc
    11401 taaagtgcgc cgtctggtga atgtcaaata tgactctttt gactctgcgc tgcgtaacgc
    11461 gccgtttatg gttgaggcgc gggcgctttc ggtagagacg gtggactcaa aagtgctgaa
    11521 tctggcgcgg gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga
    11581 caaagagatg ctcggactga acattgtgga atttgctggt gatgatgagg cgctgattga
    11641 cgagcgagtt aatgcactct gtgtgcggct tgatgagctg attgccagcc aacaggcagg
    11701 tgtgattggc tggcaggtgt gccgcgagct ggcgggggtt gaacgtatct atgcgatgcg
    11761 caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc
    11821 tgaggatacc tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct
    11881 gctcgacagc cacggcttaa gctacggtat gttcggtcac gtcgacgcag gtgtcttgca
    11941 cgtccgtccg gcactggata tgtgcgatcc gcaacaagag attttgatga agcaaatctc
    12001 tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg agcacggcaa
    12061 aggttttcgc gctgaataca gcccggcgtt tttcggtgag gaactttttg cagaactgcg
    12121 caaagtgaaa gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc
    12181 agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg
    12241 gcagatcccc attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg
    12301 tttatgcttc aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa
    12361 ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gcttgttggc
    12421 ggatcgcggc gttgacccac tcaaactgga acaagaactg cctgaatccg gcgtcagttt
    12481 gcggacgtta attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt
    12541 ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagctt gttcgaccca
    12601 gtgccccatc aaaattgatg tgccggagtt tcgctctcgt tttctgcagc tctatcacac
    12661 ccgttattta cgcccgcttc gcgaccacct cgttgctacg gtcgagagct acgcaccgct
    12721 gatggcacgc gcgccgaaaa cctttaactt cttcattaac cagccgctgg tgcgcaaact
    12781 ctcggaaaaa catatcggca tggttgattt gccactgctg tcggtcccct cgctacaaca
    12841 acaaatggtg gggcatcgct cggcaaacat gacgctggaa cagcttgaag ctctcaatgc
    12901 agagcagaaa gcgcgcacgg tgttggtggt gcaggacccc tttaccagct attacgatgc
    12961 gcaagtggtg gcggattttg tccgtctggt tgaaaaatta ggtttccagc ctgtgttact
    13021 gccattttcg ccaaatggca aagcccagca tatcaaaggc tttcttaatc gttttgcgaa
    13081 gacggcgaaa aagacggcgg atttcctcaa ccgtatggct aagcttggga tgccaatggt
    13141 gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga
    13201 ggaacgtggc gagtttaacg tcttacttgc gaatgaatgg ctggcaagcg cacttgagtc
    13261 acatccagtg gctacagtca gcggtgaatc atggtatttc tttggtcact gtaccgaggt
    13321 taccgctttg ccgggcgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa
    13381 actggaaaat gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa
    13441 aaaccatgaa aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact
    13501 gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt agccaggtaa aacgggttga
    13561 aggcacgggg gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa
    13621 cggaaaatca ccctggaagc actgaatgct atgggtgaag gaaacatggt ggggttcctg
    13681 gatattcgct ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg
    13741 cggacaaagc agcctttcgg gttgctgcat ggaggagcat ccgtggtact ggccgaaagt
    13801 atcggttccg ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa
    13861 atcaatgcta atcacgtccg ctcggcacga gaagggcggg tgcgcggcat atgtaaaccg
    13921 ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt
    13981 ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg
    14041 acgcatattg cttttggtca ttatctgcac agtaaacggt ggtatactgt gcaggtcttg
    14101 tctaaaagcg aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt
    14161 ttacgccgtg accagagcaa tctttcacct gcccaatatc tcaaacgtct gaaacaacta
    14221 gaacttgaat ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac
    14281 tttgcctggc gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga
    14341 cctggattaa cctccgtcca ggttcgatga ccttcttaat actgcttatc taccgcttga
    14401 tattgttcaa aaataatact aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag
    14461 attattctta tcgccccttc aagagctaag ccactgagag tgccggagat aagcgccgga
    14521 tggggtagaa ccccttaagc ctgtgtcgca cagacttaag ggttttctta tttctgaacg
    14581 ctgcactttt ttacctatct cttaaatagt cattttcaat gcaacttttt ctttttcatc
    14641 acagccttaa tgctccttag ataataacca ttatctaaca atgagatacc taattcttag
    14701 cgtgcctgtt aacccacaca tcaggggcta tgcttattaa aagtaggcaa aaagcatgtt
    14761 ttacggtaaa gcccctgcgt ttgctgggtt gaactgataa tcattatcac taacatgctg
    14821 ttatacgctg aaagcgatga agtgaggtaa atagatggac atgcattcag gaacctttaa
    14881 cccacaggat ttcgcctggc aaggcttaac gctgacaccc gcagcggcga tacacatccg
    14941 cgagctggtg gcaaagcagc caggtatggt cggcgtgcgc ttaggtgtga agcaaacggg
    15001 ctgcgcgggc tttggctatg tgctcgacag tgttagcgag ccggacaaag acgatctgct
    15061 gtttgaacac gacggcgcga agctgtttgt cccgctgcaa tcgatgccgt ttattgatgg
    15121 cacggaagtc gatttcgttc gtgaaggact taatcagata ttcaaatttc acaaccctaa
    15181 agcccagaat gaatgtggct gtggcgaaag ctttggggta taggcggtac tatgtctcgt
    15241 aatactgaag caactgacga tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa
    15301 ggattcttca cccagttagc caccgatgag ctggcaaagg ggataaacga agaggtggtg
    15361 cgcgcaattt cggcgaagcg taatgagccg gagtggatgc tggagtttcg tctaaacgcc
    15421 tatcgcgcat ggctggagat ggaagaaccg cactggttga aagcgcacta cgacaagctg
    15481 aattatcagg attacagcta ctactcagca ccatcgtgcg gtaattgtga cgacacttgc
    15541 gcgtctgaac ctggcgcggt gcagcaaact ggcgcgaacg cctttttaag taaagaggtg
    15601 gaggcggcgt ttgagcagtt gggcgttccc gtgcgggaag gcaaagaggt ggcggtggat
    15661 gccattttcg actctgtttc ggttgccacc acttatcgcg aaaaactggc ggagcaggga
    15721 attattttct gttcctttgg tgaggcgatc cacgatcacc cggaactggt gcgtaaatat
    15781 ctcggcaccg tggtgccggg gaatgacaac ttctttgcag cacttaatgc ggcggtagcc
    15841 tctgacggta cgtttattta tgtgcctaaa agcgtgcgct gcccgatgga actttccacc
    15901 tattttcgca ttaacgcgga aaaaaccggg cagtttgagc gcacaattct ggtggccgac
    15961 gaagacagct acgtcagcta cattgaaggc tgttccgctc cggtgcgtga cagctatcag
    16021 ttgcacgcgg cagtggtgga agtcatcatc cataaaaacg ccgaggtgaa atattccacg
    16081 gtacaaaact ggttccctgg cgataacaac accggcggta ttctcaactt cgtcaccaag
    16141 cgtgctttgt gcgaaggcga aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca
    16201 gcgattacgt ggaaatatcc cagctgcatt ttgcgcggcg ataactccat tggtgagttt
    16261 tactcagtgg cgctgaccag cggtcatcag caagcggata ccggcaccaa gatgatccac
    16321 atcggtaaaa acaccaaatc gaccattatc tcgaaaggga tctctgccgg acatagtcag
    16381 aacagttatc gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact
    16441 cagtgcgact caatgctgat tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa
    16501 tgtcgcaata acagcgcaca actggagcac gaggcaacga catcacgtat tggtgaagat
    16561 caactgtttt actgcctgca acgcgggatc agcgaagaag acgccatctc gatgattgtt
    16621 aacggtttct gcaaagacgt gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa
    16681 aaactcctcg ccatcagtct tgaacacagc gtcggataag gaataaacat gttaagtatt
    16741 aaagatttac acgtcagcgt ggaagataaa gctatcctgc gtggattaag cctcgacgtt
    16801 cgtcccggcg aagttcacgc cattatgggg ccaaacggtt cgggcaaaag taccttatcg
    16861 gcaacgcttg ccgggcgcga agattatgaa gtgacgggcg gcacggttga gttcaaaggc
    16921 aaagatttgc ttgcactgtc gccggaagat cgtgcaggcg aaggcatctt tatggccttc
    16981 cagtatccgg tggagattcc aggcgtcagt aaccagtttt tcctgcaaac ggcacttaat
    17041 gcagtgcgca gctatcgcgg gcaggaaacg ctcgaccgct ttgattttca ggatttgatg
    17101 gaagagaaaa tcgctctcct gaagatgccg gaagatttat taacccgttc ggtaaacgtt
    17161 ggtttttccg gcggcgagaa aaagcgcaac gatattttgc aaatggcggt gctggaaccg
    17221 gagttatgca ttcttgatga gtcggactcc gggctggata ttgacgcatt aaaagtggtc
    17281 gccgatggcg tgaactcgct gcgtgatggc aagcgctcat tcatcattgt tacgcactac
    17341 caacgcattc tcgactacat caagcctgat tacgttcatg tgctatatca gggacgaatt
    17401 gtgaaatccg gcgatttcac gttggtcaaa caactggagg agcagggtta tggctggctt
    17461 accgaacagc agtaatgcgc tgcaacagtg gcatcacttg tttgaagctg aagggacaaa
    17521 acgctcaccg caagcacagc agcatttaca acaattgctg cgtaccggac tgccgacacg
    17581 taaacatgaa aactggaaat atacgccgct ggaagggctg accaatagtc agtttgtcag
    17641 cattgcggga gatatatccc cacagcagcg tgatgcctta gcgttaacgt tagacgccgt
    17701 gcggctggtg tttgtcgatg gacgttacgt gcccgcgctg agcgatgcaa ttgaaggcag
    17761 cggatatgaa gtgagcatta acgacgaccg tcaggggtta cccgacgcta ttcaggcgga
    17821 agtgtttctg catttgacgg aaagcctggc acaaagcgtg acgcatatcg ccgtgaagcg
    17881 cggtcaacgg ccggcaaagc cattgctgtt aatgcatatc acccagggcg tggcaggtga
    17941 agaggtgaac actgcccatt accggcatca tctggatctg gcggaaggtg ccgaagcaac
    18001 ggtgatcgaa cattttgtca gccttaatga tgctcgtcat tttaccgggg cacggttcac
    18061 tatcaatgtc gcagcgaacg cccacttgca gcatatcaag ctggcgtttg aaaacccggt
    18121 cagtcaccac tttgcccata acgatttgtt gctggctgac gatgccaccg catttagcca
    18181 tagtttcctg ctgggtggcg cagtgttacg acacaacacc agtacgcaac tcaatggcga
    18241 aaacagcacg ctgcggatca atagcctggc gatgccggtg aaaaacgagg tgtgtgatac
    18301 ccgtacctgg ctggaacaca ataaaggttt ttgtaacagc cgacagttgc acaaaactat
    18361 cgtcagcgac aaaggccgcg cggtatttaa cggtttgatc aacgtcgcgc agcacgccat
    18421 caaaacggat ggtcagatga ccaacaataa tctgctgatg ggtaaactgg cggaagtgga
    18481 tacaaaaccg cagctggaaa tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt
    18541 ggggcgtatt gatgatgaac agatgttcta tctgcgctcg cgcgggatca atcagcagga
    18601 tgcccagcag atgatcattt acgcctttgc tgccgaactg acggaatcac tgcgtgatga
    18661 ggggcttaaa cagcaggtgc tggcccgaat cggtcaacgg ctgccaggag gtgcaagatg
    18721 actttttccg tcgacaaagt gcgggccgac tttccggtgc tttcgcgtga ggtaaacggt
    18781 ttgccgctgg cttatctcga cagcgccgcc agtgcacaga aaccgagcca ggtgattgac
    18841 gccgaggccg agttttatcg tcatggctac gcggcggtgc atcgcggtat tcatacctta
    18901 agcgcccagg cgaccgagaa aatggagaac gtgcgcaagc gggcatcgct gtttattaat
    18961 gcccgttcgg cggaagagct ggtgttcgtc cgcggcacga cggaagggat caatctggtc
    19021 gccaatagct ggggcaacag caatgtgcgg gcgggcgata acatcatcat cagtcagatg
    19081 gagcaccacg ctaacattgt cccctggcag atgctttgcg cacgcgttgg cgcagagctg
    19141 cgtgtgatcc ctctcaaccc cgacggtacg ctgcaactgg agacgctgcc tacgctgttt
    19201 gatgagaaaa ctcgcctgct ggcaattact catgtctcca acgtgcttgg cacagaaaat
    19261 ccactggcgg aaatgatcac gcttgcgcac cagcatggcg caaaagtgct ggtggatggc
    19321 gctcaggcgg tgatgcatca tccggtggat gttcaggcgc tggattgcga cttttacgtg
    19381 ttctccgggc ataaactgta tggccccacc ggaattggca ttctttatgt gaaagaagcc
    19441 ttgttgcagg agatgccgcc gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg
    19501 agtgaaggca ctacctggac caaagcacca tggcggtttg aagccggtac acccaatacc
    19561 gggggcatca ttggtcttgg cgcggcgctg gagtatgttt cggcgctggg gcttaatagc
    19621 atagccgagt atgaacagaa tctgatgcat tatgcgctat cacagctgga atctgtaccg
    19681 gatctcactc tctatggccc acaaaacagg cttggcgtta ttgcttttaa tctcggtaaa
    19741 caccacgcct atgatgttgg cagttttctc gataattacg gcattgctgt gcgtaccgga
    19801 catcactgcg ctatgccatt aatggcctat tacaacgtcc ctgcgatgtg tcgggcgtcg
    19861 ctggccatgt ataacaccca tgaagaagtg gatcgtctgg tgaccggcct gcaacgtatt
    19921 caccgtctgc tgggataaca gggaggcact atggctttat tgccggataa agaaaaattg
    19981 ctgcgtaatt ttttacgctg cgccaactgg gaagagaaat atctctacat tattgagctg
    20041 ggccagcgtc tgccagaatt acgcgccgaa gaccgaagcc cacaaaatag cattcagggc
    20101 tgccagagtc aggtgtggat tgtcatgcgc cagaatgcgc agggaattat tgaattgcag
    20161 ggcgacagcg atgcggcgat tgtgaaaggg cttattgcgg tcgtctttat tctctacgat
    20221 cagatgacgc cgcaggatat tgtcaatttc gatgtgcgtc cgtggtttga aaaaatggcg
    20281 ctcacccaac atctcacccc atctcgttca caaggtctgg aagcgatgat tcgcgcaatt
    20341 cgcgccaaag ccgctgcact tagctaaact aaagggacag ctttcatccg gttggctcac
    20401 attgccggat ggtcaatgtt tacactgacc tgcacaggag tgttttggtt tcaggtgaac
    20461 ataagcgcca tccgacgttt cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa
    20521 acgcgcgtct ttgcttacac tcacgcttat cggcgctttt agcgccatcc aggctgcctg
    20581 ggcggttgat tatccgctac caccaaccgg aagccgactg gttgggcaaa atcaaacgta
    20641 tacggtgcaa gaaggggata aaaaccttca ggctattgcc cgacgttttg atactgcggc
    20701 aatgttgatc cttgaagcca ataacactat cgccccggtg ccaaaacctg gtacgacgat
    20761 aactattcct tcacaactgt tattacctga tgcaccgcgt caggggatta tcgttaacct
    20821 tgcagagctg cgcctttatt attatccgcc gggagaaaat attgtgcagg tttatccaat
    20881 aggtattgga ttgcaggggc tggaaacgcc ggtgatggaa acgcgtgttg ggcagaaaat
    20941 ccctaaccca acctggacgc ctacggcagg cattcgtcag cgttcgctgg agcgtggcat
    21001 taaattaccg ccagtcgttc ctgccggacc aaataacccg ctaggacgtt acgcactgcg
    21061 cctcgcgcat ggtaatggcg aatacctcat tcatggcacc agtgcgccgg acagcgttgg
    21121 tttgcgcgtc agttcagggt gtattcgcat gaatgcaccg gatattaaag ccttgttctc
    21181 cagcgtgcgg acgggaacgc cggtgaaagt gatcaacgaa ccggtgaaat attccgtgga
    21241 acctaacggg atgcgttatg ttgaagtaca tcgaccacta tcagcagaag aacagcaaaa
    21301 cgttcagaca atgccataca cactgccagc aggctttacg caatttaaag acaataaggc
    21361 tgtagatcag aagttagtcg ataaagcgtt gtatcgtcgg gcagggtatc cggtttcggt
    21421 gagcagtgga gcaactcccg cagccagcaa tgcgccttca gtagagtcag cgcagaatgg
    21481 tgaaccagag caagggaata tgttacgcgc gacgcagtag tggtaaatag cagacaaaaa
    21541 aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag
    21601 tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt
    21661 cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa
    21721 cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac
    21781 ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg
    21841 agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg agaatatttt
    21901 tttgatggga aggcacttat ttttgaacgt tcgctcaaag aagcatcgaa cgctgggttt
    21961 tagataaaaa aattgagaga aaacaggatg cttccatcgg attcatctta gataaagtga
    22021 gattatatag tgaaatccga tttgattttt tattgcttct ggttatcgat taaataaaaa
    22081 aagcgcccat cagggcgctt cgatatacaa attaattcac aaaagcaata ttacaggacg
    22141 tgaacagatg cggtgttagt agtgccgctc ggtaccagtg caccagaaac cataactacg
    22201 acgtcacctt tgtgtgccag accgctctgc agagccagtt ctttacccag acggtagaaa
    22261 tcatcagtag aagtgatttc tttaaccagc tgcggcacaa cgcctttgct cagtaccaac
    22321 tgatgagccg ttttttcgtt ggtggtcagt gccaggatgg tggcatccgg gaagtattta
    22381 cgtactgcgc gagcagattt accgccctgg gtagcaacca cgatcagcgg agcatccagt
    22441 ttttcagcag tttcaacggc accacggcat accgcttcgg tgatgcgcag tttacggttg
    22501 tcattgttga actcgagacg gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc
    22561 atgatagaaa ccgcttccag cgggtattta ccttttgcgg attcaccaga cagcatcact
    22621 gcgtcagtac cgtcgaggat ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt
    22681 gggtttttga tcatggaatc cagcatctgg gtcgcagtga taacgacttt acgtgcacgg
    22741 atacattttt cgatcatcat cttctgggcg aagataactt cttctaccgg gatttctaca
    22801 cccaggtcgc cacgcgcaac catgatgccg tcagaggctt cgaggatttc gtcgaagttg
    22861 ttgaggcctt cctggttttc gattttggag atgatgtgga tgttttcgcc gccgtgcgct
    22921 ttcaggtgct cacggatttc gataacgtca gaacgcttac gaataaagga agcagcaaca
    22981 aagtctacgc ctttttcgca accaaagatc aggtcctgtt tgtctttttc agccagtgct
    23041 ggcagagcaa tggaaacgcc aggcaggttc acacctttgt tttcgcccag gtcaccgttg
    23101 ttcagcactt tacagataac tttgttacct tcaatggcgg taacttccat accgatcaga
    23161 ccatcgtcaa ccagtacggt gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt
    23221 accgcaacca tttcgctgtt gccgataaca gatttatcag tggtgaaagt aaaggtctga
    23281 ccagctttca gagaaacatc gttaccgcct tccagtttca tggtgcggat ttccggacct
    23341 ttggtatcaa gcaggatagc ggcggtttta ccagttttgc tcatcacgtt gcgcagattc
    23401 tgaatgcgct gaccgtgttc tgcatagtca ccatgagaga agttcagacg cataacgttc
    23461 atgccagcgt ccagcatttt agctaacatc tcttcagatt cggttttcgg tccgatggtg
    23521 caaacaattt tggtcttttt catgacagtc ttagtcttta agttgagaag gatgggagaa
    23581 acttgctttc tgggcgcact gccggaaagc gaatccggta ttacgaaagt ggtggtgcct
    23641 cgctctaagg ataggtgaca tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc
    23701 ccaacgtgag tgcctgtttt tgagtcgttg agttctacag tccaaagtgc tgaaaccatt
    23761 caagagtcaa ttggcgcgca ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc
    23821 gtttcaaaaa atatacaact tgttagacaa taaattgtta tcaaagattt aacgttccag
    23881 ggaaaaggtt acgcaaaaac ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg
    23941 tgaggataag tgaaagttca tccggtgtat aaatatatca tcgctcaatt gctacagtta
    24001 aaataattta tcgcgttatt tgtctgattt tgtttgattt ttgtttaatt ggttagattg
    24061 atatatgctg atagttggca tgataaatag gtataaagaa tgggaaatcg gacaaaagag
    24121 gatgaactgt accgagaaat gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat
    24181 ctggggcagg aaccaaagca tattgttatc gcaggcgtat tacgtacagc attagccaat
    24241 aaacgcattc agcgcagtga gctggaaaag caggcgatgg aaaccgtcat taacgcactg
    24301 gtaaaatgac catt
//
LOCUS       DADKXM010000053        24231 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.053, whole genome shotgun sequence.
ACCESSION   DADKXM010000053 DADKXM010000000
VERSION     DADKXM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.053"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..375)
                     /locus_tag="JQR05_002070"
     CDS             complement(<1..375)
                     /locus_tag="JQR05_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324245.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQ"
     gene            899..2827
                     /gene="thrS"
                     /locus_tag="JQR05_002071"
     CDS             899..2827
                     /gene="thrS"
                     /locus_tag="JQR05_002071"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY5324246.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2831..3373
                     /gene="infC"
                     /locus_tag="JQR05_002072"
     CDS             2831..3373
                     /gene="infC"
                     /locus_tag="JQR05_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY5324247.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3470..3667
                     /gene="rpmI"
                     /locus_tag="JQR05_002073"
     CDS             3470..3667
                     /gene="rpmI"
                     /locus_tag="JQR05_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY5324248.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3720..4076
                     /gene="rplT"
                     /locus_tag="JQR05_002074"
     CDS             3720..4076
                     /gene="rplT"
                     /locus_tag="JQR05_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY5324249.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4199..4243
                     /locus_tag="JQR05_002075"
     CDS             4199..4243
                     /locus_tag="JQR05_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY5324250.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4526..5509
                     /gene="pheS"
                     /locus_tag="JQR05_002076"
     CDS             4526..5509
                     /gene="pheS"
                     /locus_tag="JQR05_002076"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY5324251.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPSHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5524..7911
                     /gene="pheT"
                     /locus_tag="JQR05_002077"
     CDS             5524..7911
                     /gene="pheT"
                     /locus_tag="JQR05_002077"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY5324252.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQTSLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7916..8215
                     /gene="ihfA"
                     /locus_tag="JQR05_002078"
     CDS             7916..8215
                     /gene="ihfA"
                     /locus_tag="JQR05_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY5324253.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8316..9296
                     /gene="btuC"
                     /locus_tag="JQR05_002079"
     CDS             8316..9296
                     /gene="btuC"
                     /locus_tag="JQR05_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY5324254.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9359..9910
                     /gene="btuE"
                     /locus_tag="JQR05_002080"
     CDS             9359..9910
                     /gene="btuE"
                     /locus_tag="JQR05_002080"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY5324255.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9910..10659
                     /gene="btuD"
                     /locus_tag="JQR05_002081"
     CDS             9910..10659
                     /gene="btuD"
                     /locus_tag="JQR05_002081"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY5324256.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSVLDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10737..11201
                     /locus_tag="JQR05_002082"
     CDS             10737..11201
                     /locus_tag="JQR05_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5324257.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11448..12161
                     /gene="ydiV"
                     /locus_tag="JQR05_002083"
     CDS             11448..12161
                     /gene="ydiV"
                     /locus_tag="JQR05_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY5324258.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELINHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12225..13661
                     /locus_tag="JQR05_002084"
     CDS             12225..13661
                     /locus_tag="JQR05_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY5324259.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JQR05_002085"
     CDS             complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JQR05_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY5324260.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JQR05_002086"
     CDS             complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JQR05_002086"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY5324261.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JQR05_002087"
     CDS             complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JQR05_002087"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY5324262.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="JQR05_002088"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="JQR05_002088"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY5324263.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JQR05_002089"
                     /pseudo
     CDS             complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JQR05_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20495..20788)
                     /locus_tag="JQR05_002090"
     CDS             complement(20495..20788)
                     /locus_tag="JQR05_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY5324264.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="JQR05_002091"
     CDS             complement(20785..22074)
                     /locus_tag="JQR05_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5324265.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="JQR05_002092"
     CDS             complement(22130..23068)
                     /locus_tag="JQR05_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY5324266.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYDPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="JQR05_002093"
     CDS             complement(23088..23852)
                     /locus_tag="JQR05_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY5324267.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24168..>24231
                     /locus_tag="JQR05_002094"
     CDS             24168..>24231
                     /locus_tag="JQR05_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY5324268.1"
                     /translation="MYQRCFDNASETLFVAGKTPR"
BASE COUNT         5991 a         5864 c         6372 g         6004 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag agcagtggaa taacaatttt gtgatatttt
      181 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
      241 ggtatcaagt agggtgatga gctggcgcgc aaatttatta ataggctctg atgaaggtgt
      301 tggtatgtcg ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat
      361 atcgttttga ctcataatct attcgcctta tatatatctg gaggttttga ggatgatgat
      421 taattattat cgccagaacg aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt
      481 tatcattgtg atatgaccac ttcgtcatta atctcatgag cgtttaaaat actaccgcca
      541 aatcacgcaa cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa
      601 cctttcagac gcaccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
      661 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
      721 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
      781 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
      841 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
      901 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
      961 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     1021 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     1081 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     1141 tgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     1201 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     1261 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     1321 gcacgaagcg cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga
     1381 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     1441 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     1501 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     1561 ggcgtgggca gacaaaaaag cacttaacgc ttacctgcag cgcctggaag aagccgcgaa
     1621 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcgcc
     1681 gggtatggta ttctggcaca acgacggctg gaccatcttc cgtgaactgg aagtgtttgt
     1741 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     1801 tgtcctgtgg gaaaaaacag gtcactggga caactacaaa gatgcaatgt tcaccacatc
     1861 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     1921 caaccagggg ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg
     1981 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca
     2041 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     2101 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     2161 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     2221 ggcggttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     2281 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     2341 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     2401 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt ctggggtcga tggagcgttt
     2461 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccagttca
     2521 ggttgttatc atgaatatta ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa
     2581 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     2641 aatccgcgag cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt
     2701 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     2761 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     2821 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     2881 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     2941 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     3001 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     3061 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     3121 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     3181 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     3241 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     3301 agtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     3361 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     3421 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     3481 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     3541 caagcacgct aacctgcgtc acattctgac taaaaaagcg accaaacgta aacgtcacct
     3601 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     3661 cgcataagcc gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata
     3721 tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag
     3781 ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca
     3841 aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt
     3901 ggattgcgcg tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca
     3961 atggcctgaa aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat
     4021 tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca
     4081 gttgaaagag ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt
     4141 atcgccgtag ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat
     4201 gaatgctgct attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc
     4261 gtgagaagag aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg
     4321 cgcgttttga aaaataaaaa gccagtctat caggcgtaat tgtcgcagcc agtctggaca
     4381 cggccagtgc ggagaaaccg gagcgtactt aagtacgtga ggatttcgag cactgcccgg
     4441 gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt
     4501 caccactgac acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa
     4561 ggcggccatt agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt
     4621 gggtaaaaaa gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga
     4681 gcgtccggca gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa
     4741 tgcgcgtaaa gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat
     4801 tgatgtctct ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac
     4861 catcgaccgt atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga
     4921 aatcgaagac gattatcata acttcgatgc tctgaacatt cctagtcacc acccggcgcg
     4981 cgctgaccac gacactttct ggtttgacgc tacccgcctg ctgcgtaccc agacctctgg
     5041 cgtacagatc cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg
     5101 tgtttatcgt aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct
     5161 gattgttgat accaacatca gctttaccaa cctgaaaggc acgctgcacg acttcctgcg
     5221 taacttcttt gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgtttaccga
     5281 gccttctgca gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggctg
     5341 cgggatggtg catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg
     5401 tttcgccttc gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct
     5461 gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag
     5521 attatgaaat tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat
     5581 acgctggcga atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc
     5641 ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct
     5701 gacaaactgc gtgtgacaaa agtaaatgtc ggcggcgatc gcctgctgga tatcgtctgc
     5761 ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg
     5821 ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggcg aaccgtctga agggatgctg
     5881 tgctcctttt ctgagctggg aatttctgac gatcacagtg gcattatcga actgcctgcg
     5941 gatgcgccga ttggcaccga catccgcgaa tacctgaaac tcgatgataa caccatcgaa
     6001 atcagcgtga caccaaaccg tgccgactgc ttaggtatca ttggtgttgc gcgtgacgtt
     6061 gccgtgctga atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc
     6121 gacgacacgt tgccgattac agtcgaagcg ccggaagcct gcccgcgcta tcttggccgt
     6181 gtggtaaaag gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt
     6241 cgttgcggga tccgttctat cgatgcagtt gttgacgtaa ccaactatgt actgctcgaa
     6301 ctgggccagc cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg
     6361 atggcaaaag agggcgaaac gctggtgctg cttgatggca ctgaagcgaa gctgaatgct
     6421 gacactctgg tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc
     6481 gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc
     6541 ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt
     6601 tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg
     6661 ctgatcgaca tctgcggtgg tgaggctggc ccggtaattg atatcaccaa cgaagcaacg
     6721 ctgccgaagc gtgcaaccat cactctacgt cgtagcaaac tggatcgcct gataggccat
     6781 catattgctg atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa
     6841 ggcaaagacg aatggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa
     6901 gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta
     6961 cagacaagcc tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa
     7021 acgctgctca acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa
     7081 gtgcagcaga tgatccatcc aggcgttgaa gccttactgc tggcaagccc gatctctgtt
     7141 gaaatgtcag caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac
     7201 cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat
     7261 actcaggcac cgttgggcat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac
     7321 cgttacgaag agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc
     7381 gaccttgaat ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa
     7441 gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt
     7501 ggttttgttg gagttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact
     7561 ctggtgttcg aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag
     7621 atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc
     7681 gcagcggata ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac
     7741 ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc
     7801 ctgatcctgc aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc
     7861 aaatgtgtag aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc
     7921 gcttacaaaa gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga
     7981 tgccaaagaa ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga
     8041 acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg
     8101 acgtaacccg aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag
     8161 acccgggcag aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga
     8221 tctaactaaa aaggccgcta tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa
     8281 aatcaacgcc atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac
     8341 agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct
     8401 taagcctttg cgccggtgaa caatggattt cgccaggtga ctggtttact cctcgtggcg
     8461 aactgttcgt ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg
     8521 cgctggctat atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg
     8581 gactacttgg cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc
     8641 aagggcaact ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca
     8701 ctttaatact cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg
     8761 gcgttgcatt agggattatc tgcagcgcac taatgacgtg ggctatctac ttttccacct
     8821 ctgttgattt acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc
     8881 ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca
     8941 ggccgatgaa tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt
     9001 ggttctggcg caatgtgctg gtggcagcga ccggctggat ggttggcgtc agtgtggcgc
     9061 tggcgggtgc tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt
     9121 taaccgatca tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc
     9181 tggccgatat tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca
     9241 ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc
     9301 aaaaagacgg tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat
     9361 gcaagattcc attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga
     9421 gaagtacgcc ggtaatgtgc tgttaattgt caatgtcgcc tcaaagtgtg gcttaacgcc
     9481 gcaatatgag cagttggaga atattcaaaa agcctgggcc gatcgaggtt ttgtggtgct
     9541 gggattcccg tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac
     9601 ttactgtacc accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg
     9661 cgaaggacgc catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc
     9721 ggaagagagc ggattctatg cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga
     9781 tgatatttta tggaattttg aaaaattcct ggttggcagg gacggaaaag tcatccagcg
     9841 tttttccccg gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt
     9901 ggcaaaataa tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg
     9961 ccgctttcag gcgaggttcg ggctggggaa atcctgcacc tggtggggcc gaatggcgcg
    10021 ggtaagagta ccttactggc gcgaatggcc ggaatgacca gcggtaaggg aagcattcag
    10081 ttcgcggggc aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat
    10141 ctttcacaac agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat
    10201 cagcacgata aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat
    10261 gacaaactcg gtcgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt
    10321 gctgcggtgg tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt
    10381 gatgagccga tgaacagtct tgatgttgcg caacaaagtg tgttagacaa aattctgagc
    10441 gcgctgtgtc agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacgttg
    10501 cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga
    10561 gaagaggtgc tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg
    10621 gatatcgaag gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat
    10681 cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc
    10741 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
    10801 cgccgccaaa tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc
    10861 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
    10921 gttcgggatt tgtggttgtg acgatgcgcg atcgtttcga tttgcatctg ccccgtgaaa
    10981 ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc
    11041 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca
    11101 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg
    11161 tctattggca gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaattgacgg
    11221 cgatgataca tcgccgtcaa tttactcctg aatacagtag tattttgcag acaaaaattc
    11281 cgtaaatgga ttattcgttt ataatcagga taatctaaaa aaaggccccc tgaaaaagtg
    11341 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
    11401 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
    11461 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc
    11521 ttgtcggcgt tgagttaatt aaccatttct ccagtgaaga tggcacggtt cgtatcccga
    11581 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
    11641 tggaattact aaaatcatgc caacactttt ttattcaaca taaactcttt gcctggctta
    11701 atttaacgcc acaagtggct acattattat tagacaggga taattacgca ggcgaactat
    11761 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
    11821 aagataatcg cgatcttttg tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg
    11881 gagcaggtaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtaatgctgg
    11941 agaaaggttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
    12001 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
    12061 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
    12121 ccgttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
    12181 ttaagacgag agtaaccgtc tacactatca aacagatagg atctatgacc ctgtctttta
    12241 ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc actttcccct acgcctttaa
    12301 ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt
    12361 cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac
    12421 cactggcaca ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg
    12481 ggcgcggcat tttacttggc gaacaactgc ttgctgatgg cactacaatg gactggcatc
    12541 tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt
    12601 cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc
    12661 gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg
    12721 cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact
    12781 atcgccgcga gccggaaaag gttcgtcagt tggctgactt tgccattcgt cattactggt
    12841 cacatcttga agacgatgag gacaaatacc gtctctggtt taacgatgtt gtcgcacgta
    12901 ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc acatggggtg atgaataccg
    12961 acaacatgtc gctgctggga ctgacgcttg attacgggcc gtttggtttt cttgatgatt
    13021 acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc
    13081 aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg
    13141 ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg
    13201 gacaacggat gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac
    13261 tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca
    13321 tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag tttattgatc
    13381 gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa gacgaggtta
    13441 gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc agctctggta ttgcgcaact
    13501 ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgatggaa ttgcaccgcc
    13561 tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc agtcgtccac
    13621 ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt agttaacagc
    13681 agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt
    13741 ttgccatccg gacctaacag tgtctggctg gtaattcgcc ggatgggctg cggggaagcg
    13801 gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa
    13861 attaataata attgatataa caatgattct tattatcaat attatggcag gccagagcaa
    13921 gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg
    13981 cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg cgttcggtat
    14041 cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga
    14101 cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca atctccgtag
    14161 agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct
    14221 tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca
    14281 gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca
    14341 taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt
    14401 ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg
    14461 tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgctgaa gccatttcgc
    14521 ggtggatctg actttcggta gtacgagcgc caatcgcgcc ccaactgatt aaatcagcaa
    14581 taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacaccc agctcattta
    14641 cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta
    14701 aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg
    14761 tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg
    14821 cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc
    14881 gcttatcttc accattcagt attttttcaa ttctgcggcg ggaatcggtg acatgggtgg
    14941 cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa
    15001 tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc
    15061 tacggcagga ctaccggaaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa
    15121 gtgcgaggtg tgtcacacct cgccgggagg ttagcatgat aacaaaaaaa caaaccaatg
    15181 cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc ttggtggcaa
    15241 tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg
    15301 cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc
    15361 gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat
    15421 gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg
    15481 ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg ggggttttac
    15541 cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc aacgaaatgc
    15601 cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat
    15661 tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt
    15721 gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca ctttgcaaga
    15781 tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc acgcctgtct
    15841 ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa
    15901 acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata
    15961 cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat
    16021 ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac
    16081 agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcaattttat tttttatgaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccaccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aatcatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agccgcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ctacaccagt
    20101 tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaatgccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgtt gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggatcata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atggtttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt
    23941 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tggctaaaac
    24001 aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta
    24061 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
    24121 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
    24181 gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg c
//
LOCUS       DADKXM010000054        23343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.054, whole genome shotgun sequence.
ACCESSION   DADKXM010000054 DADKXM010000000
VERSION     DADKXM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.054"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..1341
                     /gene="gabP"
                     /locus_tag="JQR05_002095"
     CDS             1..1341
                     /gene="gabP"
                     /locus_tag="JQR05_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY5324269.1"
                     /translation="MLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRM
                     LAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVP
                     GIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGIYPY
                     AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRAT
                     NSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLS
                     VTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYY
                     APAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIG
                     FITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            1362..2024
                     /gene="csiR"
                     /locus_tag="JQR05_002096"
     CDS             1362..2024
                     /gene="csiR"
                     /locus_tag="JQR05_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY5324270.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(2025..2474)
                     /gene="kbp"
                     /locus_tag="JQR05_002097"
     CDS             complement(2025..2474)
                     /gene="kbp"
                     /locus_tag="JQR05_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY5324271.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVLEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(2558..2716)
                     /locus_tag="JQR05_002098"
     CDS             complement(2558..2716)
                     /locus_tag="JQR05_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY5324272.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2899..3198
                     /locus_tag="JQR05_002099"
     CDS             2899..3198
                     /locus_tag="JQR05_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5324273.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            3208..3732
                     /gene="ygaP"
                     /locus_tag="JQR05_002100"
     CDS             3208..3732
                     /gene="ygaP"
                     /locus_tag="JQR05_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY5324274.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(3779..4183)
                     /gene="stpA"
                     /locus_tag="JQR05_002101"
     CDS             complement(3779..4183)
                     /gene="stpA"
                     /locus_tag="JQR05_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY5324275.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4851..5300
                     /gene="alaE"
                     /locus_tag="JQR05_002102"
     CDS             4851..5300
                     /gene="alaE"
                     /locus_tag="JQR05_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY5324276.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(5337..5681)
                     /locus_tag="JQR05_002103"
     CDS             complement(5337..5681)
                     /locus_tag="JQR05_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY5324277.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            5833..6162
                     /locus_tag="JQR05_002104"
     CDS             5833..6162
                     /locus_tag="JQR05_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY5324278.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            6410..6655
                     /gene="nrdH"
                     /locus_tag="JQR05_002105"
     CDS             6410..6655
                     /gene="nrdH"
                     /locus_tag="JQR05_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY5324279.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPQAASA"
     gene            6652..7062
                     /gene="nrdI"
                     /locus_tag="JQR05_002106"
     CDS             6652..7062
                     /gene="nrdI"
                     /locus_tag="JQR05_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY5324280.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            7035..9179
                     /gene="nrdE"
                     /locus_tag="JQR05_002107"
     CDS             7035..9179
                     /gene="nrdE"
                     /locus_tag="JQR05_002107"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY5324281.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            9189..10148
                     /gene="nrdF"
                     /locus_tag="JQR05_002108"
     CDS             9189..10148
                     /gene="nrdF"
                     /locus_tag="JQR05_002108"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY5324282.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            10503..11705
                     /locus_tag="JQR05_002109"
     CDS             10503..11705
                     /locus_tag="JQR05_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY5324283.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            11698..12762
                     /gene="proW"
                     /locus_tag="JQR05_002110"
     CDS             11698..12762
                     /gene="proW"
                     /locus_tag="JQR05_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY5324284.1"
                     /translation="MADKNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12820..13812
                     /gene="proX"
                     /locus_tag="JQR05_002111"
     CDS             12820..13812
                     /gene="proX"
                     /locus_tag="JQR05_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY5324285.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KLGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            14004..15188
                     /locus_tag="JQR05_002112"
     CDS             14004..15188
                     /locus_tag="JQR05_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324286.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCMTFANFSILWTSMAFLLAAPPFNYSDGDIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGATGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            15312..16049
                     /locus_tag="JQR05_002113"
     CDS             15312..16049
                     /locus_tag="JQR05_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY5324287.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            16039..16374
                     /gene="ygaH"
                     /locus_tag="JQR05_002114"
     CDS             16039..16374
                     /gene="ygaH"
                     /locus_tag="JQR05_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY5324288.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(16393..16494)
                     /locus_tag="JQR05_002115"
     CDS             complement(16393..16494)
                     /locus_tag="JQR05_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324289.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            16465..16995
                     /gene="emrR"
                     /locus_tag="JQR05_002116"
     CDS             16465..16995
                     /gene="emrR"
                     /locus_tag="JQR05_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY5324290.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            17122..18294
                     /gene="emrA"
                     /locus_tag="JQR05_002117"
                     /pseudo
     CDS             17122..18294
                     /gene="emrA"
                     /locus_tag="JQR05_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            18311..19849
                     /gene="emrB"
                     /locus_tag="JQR05_002118"
     CDS             18311..19849
                     /gene="emrB"
                     /locus_tag="JQR05_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY5324291.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRLAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19913..20428)
                     /gene="luxS"
                     /locus_tag="JQR05_002119"
     CDS             complement(19913..20428)
                     /gene="luxS"
                     /locus_tag="JQR05_002119"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY5324292.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(20578..22134)
                     /gene="gshA"
                     /locus_tag="JQR05_002120"
     CDS             complement(20578..22134)
                     /gene="gshA"
                     /locus_tag="JQR05_002120"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY5324293.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGELPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(22207..22635)
                     /locus_tag="JQR05_002121"
     CDS             complement(22207..22635)
                     /locus_tag="JQR05_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5324294.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(22632..23198)
                     /gene="yqaB"
                     /locus_tag="JQR05_002122"
     CDS             complement(22632..23198)
                     /gene="yqaB"
                     /locus_tag="JQR05_002122"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY5324295.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5575 a         5952 c         5863 g         5953 t
ORIGIN      
        1 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
       61 gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact ggtggttatg
      121 attatgcgga tgctggcgga aatggcagtt gccacgcccg ataccggttc gttttccacc
      181 tatgccgata aagccattgg ccgctgggcg ggctatacca tcggctggtt gtactggtgg
      241 ttttgggtac tggttatccc actggaagcc aacatcgccg ccatgatcct gcactcgtgg
      301 gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt
      361 aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
      421 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggtat ttacccttat
      481 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggtttc
      541 ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
      601 accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc caccaactcg
      661 gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtcgt ggcattaata
      721 ccgtggaata tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc
      781 aatattcccc atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc
      841 ctgaactcgg ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat
      901 gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc ggtgttactc
      961 tccaccggcg cggcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt
     1021 aaatttctga tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt
     1081 tcacagttgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg
     1141 tggctttatc cctggctcac ctggctggtc atcggcttta ttacctttgt gttggtagtg
     1201 atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg
     1261 attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca
     1321 cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg
     1381 atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat
     1441 tacggatgag tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagcac
     1501 tttctcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg
     1561 cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga
     1621 tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa
     1681 aagcgcatct gctcagtaag ctggaagcct gtgacgccag cgagaaaatg cttgatgagt
     1741 gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt tctcactatt
     1801 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ttatcgattt atctggctgc
     1861 ggcgaacggt gctttcggtg gaaatgctgg aggataaaca cgatcagcac cagaccctga
     1921 ctgcggcggt actggcgcga gatacagcgc gcgccagtga gttaatgcgc cagcatttac
     1981 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata
     2041 cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg
     2101 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
     2161 tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga
     2221 tcatcaacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt ctccttcgcc
     2281 tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact
     2341 ttatcggcat caggtatacc ggttttgctc agatgctcca gcaccttctt cgcctgatcg
     2401 tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa
     2461 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
     2521 aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga
     2581 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
     2641 cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg gtgatgacga
     2701 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
     2761 ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcttac gcttttactc
     2821 tcatttacgc tgctgaggct ggtatatgtt gcatttttag tttagagttt acttaattta
     2881 gaaaacactt aaataaatat gactgaactc gcgcaattac aggccagtac cgaacaggca
     2941 gcggccttat tgaaagcaat gagccaccct aaacggttgc tgattctgtg catgcttagc
     3001 ggttcccccg gcaccagcgc gggagagctg acgcgcatta ccggactgag cgcctctgcg
     3061 acatcacagc atctcgctcg tatgcgggac gaagggctta tcaacagcca acgggatgcc
     3121 caacgcattc tatattccat taaaaatgag gcggtaaatg ccattatcgc caccctgaaa
     3181 aatgtctatt gtccgtaagg agtcactatg gctttgacaa ccatttcgcc gcatgatgca
     3241 caagaattaa tcgcacgcgg cgcaaagtta atcgatattc gtgacgctga tgaatatctt
     3301 cgtgaacata ttcctgaagc agatctggct ccattatccg tgctggaaca gtcaggtctt
     3361 ccacctaaat tacgtcgcga gcaaattata ttccactgcc aggcaggtaa acgcaccagt
     3421 aataacgccg ataaattagc agcgattgcc gcccccgcag aaatattttt actcgaagat
     3481 gggattgatg gctggaaaag agctggattg ccagtagcgg taaataaatc tcaacccttg
     3541 ccgttaatgc gccaggtgca gatcgctgcg ggtggtttaa tattaatcgg cgttgtactg
     3601 ggttataccg taaatagcgg tttcttctta ttaagtggct ttgttggtgc cgggttactg
     3661 tttgcaggaa tcagcggttt ttgtggaatg gcaaggttgt tagataagat gccgtggaac
     3721 caacgagctt gagaagcgac gccggacgcg ccctagcagc gacatccggc ctcagtaatt
     3781 agatcaggaa atcgtcgaga gatttacctt ctgccagcgc ctgagcaatt ggcttcggtg
     3841 tacggccctg accggtccag gttttagttt caccgttaac atcagtgaat ttatatttcg
     3901 ccggacgcgg ctggcgtttt ttaccagcac gtggtgcagc agcagagcta ttacccaata
     3961 actcttccgg gttaattccg tcagctttca tcagctccag ccaggtgcta attttttcct
     4021 gacgctcagc cagttcacgc tgctgctgtt cttcttcttc acgtctttct ttagtgacaa
     4081 ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga gccatcgcac
     4141 ggagggtgcg aatgttattt aaactttgta acattacgga cataataaag aaaacctttt
     4201 aacgccaaaa caaaaaagta tttcgtcgcg tagtgtaagc gatcacttat tatatttcca
     4261 caaaatttaa tctatttaca gtcctgcgcg agattatttc agctgttatt ttctgaaact
     4321 ctcagaataa tttcaagatt atatattagc cattttgatg agtaatttag gttattggtg
     4381 aacagagtgg cgctctctgc tcgctaactc cgcaagcctg acaaaaccga agtgaataaa
     4441 aatgcaatat aacagaataa aagaaaacac ccctcactgg tgctttctgc tgttaatgta
     4501 cattttctgg catcaaaaat gctgtaaatg acccggtcac gacaaacacc gaattctcta
     4561 cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc aaggccatat
     4621 ccaattgatt taattgaaaa taaatcatat cagcatgtaa aaatccacaa tgtacaaaaa
     4681 acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt tttcactaat
     4741 tgattagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat gagatccact
     4801 gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc atgttctcac
     4861 cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt tactgttctg
     4921 tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag tctttttatt
     4981 ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt atgtaccgcg
     5041 atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa aatctggctg
     5101 atatcctagc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg
     5161 gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt tcgatgttga
     5221 tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa gtcagccgtt
     5281 accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc gcgtctttaa
     5341 ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga actaaagcca
     5401 tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa
     5461 tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat
     5521 gttggatgaa ttaccagagc ggtacgtccc attcgcgctt cgcgattaac atagacataa
     5581 ttttccccac ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact
     5641 ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttatttt
     5701 caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa agtccataaa
     5761 gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca tcgaattcta
     5821 atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat
     5881 atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa gtcctggggc
     5941 agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcgtt gctgaaagaa
     6001 accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcgcgcga tgccgttggc
     6061 tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc agctgcggta
     6121 ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa
     6181 tacgcgtctt tgaccccgaa gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa
     6241 aatccccctc ccccgtcacg ctcatatcca gggtaatttc gatcactatt tgctatatat
     6301 tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag agagacaacc
     6361 cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca tgcgcataac
     6421 tatttatact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg
     6481 gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg aagcgttgcg
     6541 tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct ggtctggttt
     6601 ccgtccggac atgattaacc gtctgcatcc agcgccacaa gcggccagtg catgagccag
     6661 ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg tttaggtctg
     6721 cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga gccttacatc
     6781 ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg acaggtaatt
     6841 cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc ttctggtaat
     6901 cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg
     6961 ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa
     7021 ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa acgatggatt
     7081 accacgcgct gaatgcgatg cttaatcttt acgatagcgc aggtcgcatt cagttcgata
     7141 aagaccgcca ggccgttgac gcctttattg cgacgcatgt gcgtccgaac agtgtgacct
     7201 tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat gatgaaagcg
     7261 ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca cacaccagcg
     7321 gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat acgttgaaga
     7381 cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg gtggcgctga
     7441 cgctggcaca aggcgatgag acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac
     7501 gctttcagcc agccacgcca acattcctca actgcggtaa gcagcagcgc ggcgaactgg
     7561 tttcctgttt tttgctgcgt attgaagaca atatggagtc gattggtcgg gcggtaaatt
     7621 ccgcactgca gctgtcgaaa cgcggcggcg gcgtagcatt tttgctgtcg aatctgcggg
     7681 aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga
     7741 aaatgttgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag ggggcaggtg
     7801 cagtctattt acatgctcat catcccgata ttctgcgttt tctcgacacg aaacgggaaa
     7861 atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc ccggatatca
     7921 ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc
     7981 gagtttatgg caagccgttt gccgatatcg ccattagcga acactatgac gaactggttg
     8041 ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag cgactggcag
     8101 aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacagta aaccgtgcta
     8161 accctatcgc cgggcgcata aatatgagta atctctgctc agaaattttg caggttaaca
     8221 gcgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat atttcctgca
     8281 atttaggttc gttgaatatt gcgcacacca tggattcccc cgatttttcc cgcacggtag
     8341 agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc agcgtgccgt
     8401 ccatcgaagc cggaaatgcc gcctcgcacg ccatcggact ggggcagatg aatttacatg
     8461 gttatctggc gagggaaggc atcgcttatg gttcgccgga agccctggat ttcaccaatc
     8521 tctatttcta taccatcacc tggcacgcac tgcgtacctc gatgttgctg gcgcgcgaac
     8581 gcggtgaaac cttcgccggg ttcaaacagt cacgctatgc cagtggtgaa tatttcagcc
     8641 aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg tttgcccgca
     8701 gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct
     8761 acggcatata caaccagaat ctccaggcgg tgccgccaac cgggtctatc tcttatatca
     8821 accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc aaagagggca
     8881 aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg gcgctgtatc
     8941 aggacgctta cgaaattggc gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc
     9001 atgtcgatca ggggctgtcg ctgacgctgt ttttccccga taccgccacc actcgcgata
     9061 tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat tacatccgcc
     9121 tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag
     9181 gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata tctgacgata
     9241 aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa aaggtgccgc
     9301 tgtcgaacga tattcccgcc tggcagacat taactgtcgt agaacaacaa ctgacgatgc
     9361 gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc
     9421 tgatgcccga tgcgctcacg cctcatgaag aggcggtatt atcgaatatc agctttatgg
     9481 aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag accaaagatg
     9541 tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta
     9601 ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg
     9661 aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc cgcggaaagc
     9721 tgaccaatac cgcggacctg atccgtctga ttatccgcga tgaagcagtc cacggttact
     9781 acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt
     9841 tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagttg caatacaccg
     9901 atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc tgttacaaca
     9961 ccaataaggc tttgatgaat ctgggctacg aaccgttatt tcccgcagaa atggcggaag
    10021 tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac gatttctttt
    10081 ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat gaagactgga
    10141 atttctgagg gtgttatttt caaaaatatc actacccgca gcagggaaat aattcccgcc
    10201 aaatagcttt ttatcacgca aatattctgt agtgatctac actgatactc tgttgcatta
    10261 ttcgcctgaa actacaatat tcaggcgttt tttcgctatc tatgacaaaa aatatcaact
    10321 tttctcgatt tgctctcagc ccttatatca cggaaaattc cggcgatttg ctcgcatcaa
    10381 tattcatgcc acatttgccc tcaggggttg cctcagattc tcagtatgtt agggtagaaa
    10441 aaagtgacta tttccattgg gtcatatatc gacataaaca aataaaggaa tcattctatt
    10501 gcatggccat taaattagaa attaaaaatc tttataaaat atttggtgag catccacagc
    10561 gagcgttcaa atatatcgaa caaggacttt caaaagaaca aattctggaa aaaactgggc
    10621 tatcacttgg cgtaaaagac gccagtctgg ccattgaaga aggcgagata tttgtcatca
    10681 tgggattatc cggctcgggt aaatccacaa tggtacgcct tctcaatcgc ctgattgaac
    10741 ccacccgcgg gcaagtgctg attgatggtg tggatattgc caaaatatcc gacgccgaac
    10801 tccgtgaggt gcgcagaaaa aagattgcga tggtcttcca gtcctttgcc ttaatgccgc
    10861 atatgaccgt gctggacaat actgcgttcg gtatggaatt ggccggaatt aatgccgaag
    10921 aacgccggga aaaagccctt gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca
    10981 gctacccgga tgaactctct ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag
    11041 cgattaatcc ggatatatta ttaatggacg aagccttctc ggcgctcgat ccattaattc
    11101 gcaccgagat gcaggatgag ctggtaaaat tacaggcgaa acatcagcgc accattgtct
    11161 ttatttccca cgatcttgat gaagccatgc gtattggcga ccgaattgcc attatgcaaa
    11221 atggtgaagt ggtacaggtc ggcacaccgg atgaaattct caataatccg gcgaatgatt
    11281 atgtccgtac cttcttccgt ggcgttgata ttagtcaggt gttcagtgcg aaagatattg
    11341 cccgccggac accgaatggc ttaattcgta aaactcctgg cttcggccca cgttcggcac
    11401 tgaaattatt gcaggatgaa gatcgcgaat atggctacgt tatcgaacgc ggtaataagt
    11461 ttgtcggcgc agtctccatc gattcgctta aaaccgcgtt aacgcagcag caaggtcttg
    11521 atgcggcgct gattgatgcg ccgttagcag tcgatgcaca aacgcctctt agcgagttgc
    11581 tctctcatgt cggacaggca ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg
    11641 tcggcatcat ttcgaaagga atgctgctgc gcgctttaga tcgtgagggg gtaaataatg
    11701 gctgataaaa ataatccgtg ggataccacg ccagcggcgg acagtgccgc gcaatccgca
    11761 gacgcctggg gtacaccgac gactgcaccg gctgacggcg gtggtgctga ctggctgacc
    11821 agtacgcctg cgccaaacgt cgagcatttt aatattctcg atccgttcca taaaacgctg
    11881 atcccgctcg acagttgggt cactgaaggg atcgactggg ttgttaccca tttccgtccc
    11941 gtcttccagg gcgtgcgcgt tccggttgat tatatcctca acggtttcca gcaattgctg
    12001 ctgggtatgc cagcgccagt ggcgattatc gttttcgctc tcatcgcctg gcagatttcc
    12061 ggggtcggaa tgggcgtggc gacgctggtc tcgctgattg ccatcggcgc aatcggtgcc
    12121 tggtcgcagg caatggtgac tctggcgctg gtgttaactg ccctgctgtt ctgtatcgtc
    12181 atcggtttgc cgttggggat atggctggcg agaagtccgc gagcggcgaa aattattcgt
    12241 ccactgcttg atgccatgca gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg
    12301 ctatttggta tcggtaacgt gccgggcgtg gtggtgacga tcatctttgc gctgccgccg
    12361 attatccgtc tgaccattct ggggattaac caggttccgg cggatctgat tgaagcctcg
    12421 cgctcattcg gtgccagccc gcgccagatg ctgttcaaag ttcagttacc gctggcgatg
    12481 ccgaccatta tggcgggcgt taaccagacg ctgatgctgg ccctttctat ggtggtcatt
    12541 gcctcgatga ttgccgtcgg cgggctgggt cagatggtac ttcgcggtat cggtcgtctg
    12601 gatatggggc ttgccaccgt tggcggcgtc gggattgtga tcctcgccat tatcctcgat
    12661 cgcctgacgc aggccgttgg gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc
    12721 actggccctg ttggtctgct gacccgccca ttcattaagt aactctgcac ttgcccggtg
    12781 acgccgggca ttatcaccct gccaaaaaaa ggaataacaa tgcgacatag cgtacttttt
    12841 gcgacagcgt ttgccacgct tatctctaca caaacttttg ctgccgatct gccgggcaaa
    12901 ggcattactg ttaatccagt tcagagcacc atcactgaag aaaccttcca gacgctgctg
    12961 gtcagtcgtg cgctggagaa attaggttat accgtcaaca aacccagcga agtggattac
    13021 aacgttggct acacctcgct tgcttccggc gatgcaacct tcaccgccgt gaactggacg
    13081 ccactccatg acaacatgta cgaagctgcc ggtggcgata agaaatttta tcgtgaaggg
    13141 gtatttgtta acggcgcagc acagggttac ctgatcgata agaaaaccgc cgaccaatac
    13201 aaaatcacca acatcgcaca actgaaagat ccgaagatcg ccaaactgtt cgataccaac
    13261 ggcgacggta aagcggattt aaccggttgt aaccctggct ggggctgcga aggtgcgatc
    13321 aaccaccagc ttgccgcgta tgaactgacc aacaccgtga cgcataatca ggggaactac
    13381 gcagcgatga tggccgacac catcagtcgc tacaaagagg gcaaaccggt gttttattac
    13441 acctggacgc cgtactgggt gagtaatgag ctgaagctag ggaaagatgt ggtctggttg
    13501 caggtgccgt tctccgcact gccgggcgat aaaaatgccg ataccaaact gccgaatggc
    13561 gcgaattacg gcttcccggt cagcaccatg catatcgttg ccaacaaagc ctggaccgag
    13621 aaaaatccgg cggcagccaa actgtttgcc attatgcagt tgccagtggc cgatattaac
    13681 gcccagaacg ccattatgca tgacggcaaa gcctcagaag gcgatattca gggccacgtt
    13741 gatggctgga tcaaagccca ccagcagcag ttcgatggct gggtgaatga ggcgctggca
    13801 gcgcagaagt aatttttatt cgggcggata aggcgttttc gcctcatccg ccgctctgta
    13861 cacaatgcct gatgcgacgc tgacgcgtcc tatcatgcct acaaaccaca tcgcacattt
    13921 caacaagcta tctttcatcc catattcatc aacatccgct attattgatt tccagcttaa
    13981 tcatcacctg atgaacaaaa ataatgacta aacctaatca tgagcttagc ccggcgctga
    14041 tcgtgctgat gtctatcgcc accggtctgg cggtagccag taactattac gcccagccat
    14101 tgctcgacac catcgcgcgt aacttttccc tttccgccag ttcggcaggc tttattgtta
    14161 ccgccgcgca gttgggctat gccgcaggtc tactgtttct tgttcccctc ggtgatatgt
    14221 ttgaacgccg ccgcctgatt gtctcgatga ccttactggc agcgggcggt atgttaatca
    14281 ctgccagcag tcagtcactg gcgatgatga tcctcggtac ggcattaacc ggtttattct
    14341 cagtcgtggc acaaattctg gttccgctgg cagcgacgct ggcttcaccg gataaacgcg
    14401 gcaaagtggt tggcaccatt atgagcggtc tgctgttggg gatattgctg gcacgaaccg
    14461 ttgccgggtt actggcaaac ctcggcggct ggcgcaccgt cttttgggtt gcttcggtgt
    14521 taatggcact gatggcgctg gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc
    14581 acctcaacta cccacagttg ttgggttccg ttttcagcat gttcatcagc gataagatcc
    14641 tgcgcacccg cgcgttgctg ggctgcatga cctttgccaa cttcagcatt ctctggacct
    14701 caatggcctt tttgcttgcc gctccacctt ttaactacag cgatggtgac attggtctgt
    14761 ttgggcttgc gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgaca
    14821 agggcaaatc gcaccacacc acaactttcg gtctgcttct gctgttactt tcatggctgg
    14881 caatctggtt tggtcacact tccgtactgg cgttgattat cggcattctg gtgctggacc
    14941 tcaccgtaca gggcgtgcat atcactaacc agacggtaat ttatcgaata catcctgatg
    15001 cgcgtaatcg cctgaccgca ggttacatga ccagctactt tattggcggt gccaccggtt
    15061 cgctaatttc agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg
    15121 cgacgattgc cctggttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg
    15181 caaattaagc aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc
    15241 catcagtctg ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa
    15301 gcgtacactc tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg
    15361 aaggatgcaa agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg
    15421 gtctgaatgc gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca
    15481 tttatgcagg cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt
    15541 gggttgctgc actgaccgtc atggcaatgg atgttcgcca tgttttgtat ggcccgtcac
    15601 tgcgtagccg tattattcag cgtctgcaaa aatcgaaaac cgccctatgg gcgtttggcc
    15661 tgacggatga ggtttttgcc gccgccaccg caaaactggt acgcaataat cgccgctgga
    15721 gcgagaactg gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg
    15781 taataggggc attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat
    15841 taggttttat gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac
    15901 aatctctttg cgttaccgca gctttagttg gtgcccttgc aggcgtaacg ctattttcta
    15961 ttcccgtcgc cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat
    16021 tctggcaagg agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg
    16081 gcgaattatt gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc
    16141 aaacgtggcg cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg
    16201 ctggttgtct ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg
    16261 gtcggcttcg cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca
    16321 ctgcttagtg cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca
    16381 agaatcattt ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc
    16441 tgaaattaat gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta
    16501 aaatttcgcg ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt
    16561 tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg
    16621 attaacgaga cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt
    16681 cagccttctg aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc
    16741 gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc
    16801 ctgcatctgc aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag
    16861 cataactgcc tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag
    16921 caaatcaccc gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc
    16981 gaagcgatga gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga
    17041 ccggccagca tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt
    17101 aagaagatcg tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg
    17161 gtaaagaaga gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt
    17221 attgccgtag cgatagggat ttattggttt ttggtactgc gtcatttcga agaaaccgat
    17281 gacgcatacg tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg
    17341 aaagtctggg ccgataacac cgattttgta aaagaaggcg atgttctggt cactctcgac
    17401 ccgacagatg ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc
    17461 caaacccacc agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa
    17521 atcgccctcg cgaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac
    17581 ctgattggtc gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa
    17641 ctggacgtcg ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg
    17701 gaagatcagc ctgccgtgca acaggctgcc accgaagtac gtaacgccta gctggcgctg
    17761 gagcgtaccc gtattgtcag tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg
    17821 ggtgcgcaaa ttagcccaac gacgccgctg atggcggtcg ctccagccac caatatgtgg
    17881 gtggatgcca actttaaaga gacgcagatt gccaatatgc gcatcggtca gccggtcacc
    17941 atcaccaccg atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat
    18001 atgggcacag gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc
    18061 aaagtcgttc agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac
    18121 ccgctgcgca ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag
    18181 gtactggcaa ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc
    18241 ctggcacctg tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga
    18301 ggtgcgtgtg atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat
    18361 tgcgctgtca ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat
    18421 ccccactatc gccgggaatc tgggctcatc gctcagccag ggaacgtggg taatcacttc
    18481 tttcggggtg gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg
    18541 ggaagtgaaa ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg
    18601 tgtctccagc agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg
    18661 gccgttgatc ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc
    18721 gatcgcgctg gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct
    18781 cggcggttat atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat
    18841 tggcgtggcg gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga
    18901 acggcggcgg attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca
    18961 gattatgctc gaccgcggta aagagctgga ctgtttttca tcacaggaaa ttatcattct
    19021 taccgtggtg gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa
    19081 cccgatagtc gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat
    19141 cagcctcgcg tatatgctct acttcggcgc tattgttctg ctgccgcagt tgttgcagga
    19201 ggtctacggt tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc
    19261 ggtgattctg tcgccgatta tcggccgcct cgcgcataaa ctggatatgc ggcggctggt
    19321 aaccttcagc tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc
    19381 aggtatggat tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg
    19441 cttctttatg ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc
    19501 ggcatcgagc ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac
    19561 cacgaccatg tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa
    19621 cccgttcaac ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca
    19681 acagcaggca tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc
    19741 caatgagatc ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt
    19801 tgctaaaccg ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag
    19861 ccagttcatt tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt
    19921 gcagttcctg caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacgt
    19981 cacgttccag aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag
    20041 tgccacactg gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt
    20101 cttccattgc cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac
    20161 tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac
    20221 cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt
    20281 ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc
    20341 catgcggggt gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat
    20401 cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt
    20461 tctgaactct ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc
    20521 atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca
    20581 ggcgtgtttt tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg
    20641 acggcgttca gatgcctcgc gctcggctac aaaatcctct tcccgcagaa tttccagcgg
    20701 ctcttcacgc agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc
    20761 agtatcaatc atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca
    20821 ggcaaccagt tcatcacaca ctttctgata cgcttcgccg ccgttaatgc tatccagcgt
    20881 ttgcgcgacg cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg
    20941 cgcggtttcg cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg
    21001 gttccagtta acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag
    21061 cgcacaccag accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg
    21121 cgagaacggg ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag
    21181 cgcatcagaa ggtaactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag
    21241 ttcgttttca atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat
    21301 accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta
    21361 ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag
    21421 atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc
    21481 aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc
    21541 aaacagataa ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc
    21601 cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa
    21661 tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta
    21721 gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta
    21781 ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat
    21841 ccgctcatcg cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat
    21901 atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt
    21961 agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag
    22021 tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa
    22081 cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac
    22141 ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc
    22201 ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg
    22261 taacgcttta ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc
    22321 cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc
    22381 accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg
    22441 aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt
    22501 taaaactaaa acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc
    22561 ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga
    22621 taacgcttca ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc
    22681 tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc
    22741 tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg
    22801 acgacggcgt caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg
    22861 ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca
    22921 acaagaggaa gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca
    22981 cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata
    23041 cgccaggtgg gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag
    23101 tgccctaata cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca
    23161 tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa
    23221 tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat
    23281 tcaggaaatg gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga
    23341 gaa
//
LOCUS       DADKXM010000055        21735 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.055, whole genome shotgun sequence.
ACCESSION   DADKXM010000055 DADKXM010000000
VERSION     DADKXM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.055"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..788)
                     /locus_tag="JQR05_002123"
     CDS             complement(<1..788)
                     /locus_tag="JQR05_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY5324296.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSE"
     gene            1136..2008
                     /gene="rluF"
                     /locus_tag="JQR05_002124"
     CDS             1136..2008
                     /gene="rluF"
                     /locus_tag="JQR05_002124"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY5324297.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2141..2413)
                     /locus_tag="JQR05_002125"
     CDS             complement(2141..2413)
                     /locus_tag="JQR05_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY5324298.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLNNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2666..4015)
                     /gene="lysC"
                     /locus_tag="JQR05_002126"
     CDS             complement(2666..4015)
                     /gene="lysC"
                     /locus_tag="JQR05_002126"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY5324299.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4540..6189
                     /gene="pgi"
                     /locus_tag="JQR05_002127"
     CDS             4540..6189
                     /gene="pgi"
                     /locus_tag="JQR05_002127"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY5324300.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQGLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            6651..6899
                     /locus_tag="JQR05_002128"
     CDS             6651..6899
                     /locus_tag="JQR05_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324301.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTTTSTQ"
     gene            7013..7651
                     /locus_tag="JQR05_002129"
     CDS             7013..7651
                     /locus_tag="JQR05_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY5324302.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            7648..8385
                     /locus_tag="JQR05_002130"
     CDS             7648..8385
                     /locus_tag="JQR05_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY5324303.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRVPQ"
     gene            8385..10481
                     /locus_tag="JQR05_002131"
     CDS             8385..10481
                     /locus_tag="JQR05_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY5324304.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(10528..10806)
                     /locus_tag="JQR05_002132"
     CDS             complement(10528..10806)
                     /locus_tag="JQR05_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324305.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            10807..10950
                     /locus_tag="JQR05_002133"
     CDS             10807..10950
                     /locus_tag="JQR05_002133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324306.1"
                     /translation="MFQRIIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11076..11486
                     /gene="psiE"
                     /locus_tag="JQR05_002134"
     CDS             11076..11486
                     /gene="psiE"
                     /locus_tag="JQR05_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY5324307.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11530..13005)
                     /gene="xylE"
                     /locus_tag="JQR05_002135"
     CDS             complement(11530..13005)
                     /gene="xylE"
                     /locus_tag="JQR05_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY5324308.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(13377..14267)
                     /gene="malG"
                     /locus_tag="JQR05_002136"
     CDS             complement(13377..14267)
                     /gene="malG"
                     /locus_tag="JQR05_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY5324309.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(14282..15826)
                     /gene="malF"
                     /locus_tag="JQR05_002137"
     CDS             complement(14282..15826)
                     /gene="malF"
                     /locus_tag="JQR05_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY5324310.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(15980..17170)
                     /gene="malE"
                     /locus_tag="JQR05_002138"
     CDS             complement(15980..17170)
                     /gene="malE"
                     /locus_tag="JQR05_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY5324311.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            17535..18650
                     /gene="malK"
                     /locus_tag="JQR05_002139"
     CDS             17535..18650
                     /gene="malK"
                     /locus_tag="JQR05_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY5324312.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18722..20062
                     /gene="lamB"
                     /locus_tag="JQR05_002140"
     CDS             18722..20062
                     /gene="lamB"
                     /locus_tag="JQR05_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY5324313.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            20214..21134
                     /gene="malM"
                     /locus_tag="JQR05_002141"
     CDS             20214..21134
                     /gene="malM"
                     /locus_tag="JQR05_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY5324314.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            21363..>21735
                     /locus_tag="JQR05_002142"
     CDS             21363..>21735
                     /locus_tag="JQR05_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324315.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMLREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDFDLTNCHFKGDVISKV"
BASE COUNT         5455 a         5543 c         5584 g         5153 t
ORIGIN      
        1 ttttcgctca tattcgtttc aaccattgcg ccgtgaatat caaaaaagcg cgagcaaata
       61 tcgccagcaa cctggcgggc attcaggtcg tcactctctt caccaccata aaacgcatgc
      121 cagttagcgc cgtcgcgaat ggccggtgag ccaattccca ccagggcgac atccagatta
      181 tcccagtagg cagagatggt tttaaagtgc tgagactgca tgatcccatt acgaattaat
      241 gggttatcca gcagagccgg aaaatccgcg agatgcgatt cccctttcag cttcgctgcc
      301 gcgctgtagg ttaatgtgtt tacatgatag cggctttcga gtttaccgga cggtccgcca
      361 ataattggta cgcagattaa ctgccgcgat tgccccgcct gcggcaagtt ttcaaccagt
      421 gcgctcaccg cgcgacccca ggaaaaaccg acaatatcgc caggctccag caagcgatcc
      481 agcagttgcg cgccgtgtaa ccccatcatc gccagttgag tctcttcatc ctcatcattt
      541 cccgacacca ccacaacgtc tttcaggcca aacttttgct tcagttgctg ctccagccag
      601 agattttcgt tgtagtcata gttgatggcg atggtgacca ttccctgatc gcggcctcgt
      661 ttaagcaagc ggctgatggt ggtgcggtaa atacccagtt cgcgcgcgat ttgcgcctgc
      721 gtcatatcct gttcgtaata gagttgggca atcttcacaa tcaaacggat atcgtcactg
      781 ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct caccgcttga
      841 tctgatataa ccccatcatg agggcctttc aaagcgattt ttcactcttt gcatggggag
      901 aaatagcgct ttctcttgca catttgtttc tctgcgtagt agtcatcagc acaggtgtgc
      961 agcgattcgt aatatgttga ttatccttat tttgccaggc aaatttgcat cgtacttccg
     1021 gagacacttt tgtgataaaa ggcgtggcag atcacattta atttcgacgt tgccgcataa
     1081 ccctgttttt ccccgctaag aggtacggta tattccgcgt tttacaggag aatttatgct
     1141 gcccgactca tcagtccgtt taaataaata catcagcgaa agcggaattt gctcacgccg
     1201 cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc gagccaccat
     1261 tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac ggtcagttga ttgagcctcg
     1321 ggaagccgaa gatttggtac ttatcgccct gaacaagccc gtcggtattg tcagcaccac
     1381 cgaagatggc gagcgcgata acattgtcga tttcgttaac cacagcaaac gcgtgttccc
     1441 gattggccgc ctggataaag actcccaggg gctgattttc ctcaccaatc acggcgatct
     1501 ggtgaataag atcctccgtg cgggtaacga tcatgagaaa gagtatctgg tgacggtcga
     1561 taaaccgatt accgatgaat ttattcgtgg tatgggggcg ggggtgccga tcctcgggac
     1621 ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt gtcttccgca ttaccctagt
     1681 gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat ttcggctatg aagtgaaaaa
     1741 gctggaacgt acgcgcatta tgaacgtcag cttaagcggc attccgctgg gggagtggcg
     1801 cgatttaacc gacgatgagt taatcgacct ctttaagctc atcgaaaatt cctcttccga
     1861 ggcaaaacct aaagcgaagg ccaaaccgaa aacagcgggc atcaaacgtc cagtcgttaa
     1921 gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc aacggtaagc gttttacctc
     1981 gccggggcgt aaaaagaagg gacgctgatt aacgcctcag actgatgaaa acgtccaaaa
     2041 agagctcgga cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaagattgtg
     2101 tccacacaga cgagtgaaaa tctcaggcgt tgattgttca ttagcgcctg ccgcgcgtcg
     2161 acgtatttgc tgaccagcta aatgacgctt caggatccgg tttataagcc tgctttttct
     2221 tcaattggcg ggcttttttc gcttcagcct cacgctcgac cattaattta tcgatgtact
     2281 ctttcttaat gctgttggtt tcggagttgt tcagtacgcg accatgcgca atccgcgccc
     2341 gatccagcag tgttttaagt tcacgctgct cgcgttcagt catctctttt tgggtaatac
     2401 gggggagtgc catgatggtg ccctcagtga gccagagagt tcagtgtacg ggaattcgtt
     2461 tgcgagcaga actgataata ttcattttgc cttctcttaa ctatttatct ctttcgataa
     2521 ttaattagag atgcagataa aaaaattaaa ggcaattatt ctccgataaa gacgttggtt
     2581 aacagagtac aggctcgttt attgatgagc atagtgacaa gaaaatcaat acggcccgaa
     2641 atatagcttc caggccatac agtatttact caaacaaatt actatgcagt ttttgcacca
     2701 cctgctcggc atcttcgccg ggcaccagga agcacaggtt atggctggat gcgccgtaac
     2761 aaatcatgcg aatgttgaac ggttccagta cgccgaatac ctctttgcca acgccgcagg
     2821 cttttgacag gtcattgcca atcaacgcga ccagcgccag accttcttcc acctccaccc
     2881 ggcacagtgc ggaaagctcc atcagcagag attgcgtcag caacgtatcg ccagtggagg
     2941 ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc tgacgtggtg attaagtcta
     3001 ccgaaatatt atgccgcgcg aggatgccga aaacttccgc gaggaagccg cgagaatgca
     3061 gcatattcag gctgtgcaaa gtgagcagag tctgattgcg acgaagcgcc agcgcgcgga
     3121 acagcggcgg attttcagtt ttattgcaca ccagcgtacc acctgcgcgt gggtctttgc
     3181 tggagccgac aaagaccggg atatcgctgc gtactgcggg tagcaacgtt gccggatgca
     3241 gtacttttgc accaaaagtt gccatctctg ccgcttcggc aaacgcgatt tcatcaatgc
     3301 gttttgctgc ggaaactacg cgtggatcgg tggtgtagat gcccgggaca tcggtccaga
     3361 tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc tgccgtataa tcgctgcctc
     3421 cacggccaag cgtcgttgta cgacctttat tttcgctacc gataaatccc tgggtgatca
     3481 ctaagccttc attgaggcgt gggagcagct gcagcgcggc cagttccgcc agcgcggcta
     3541 catctggctc tgcacgacca aatcggtcgt tggtacgcat cactttacgt acatcaaacc
     3601 actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa cagcagggtc gacatcagct
     3661 cgccgtggct gaccagctca tctgtcagcg ccggagacgt tgccagcgcc gccgcttctg
     3721 ccagaacagt aatgttctcc agcagacgtt caatctcttc acggataacg ttcgggtaac
     3781 gcagacgttc cagaatggca aactggatgt tgcgaatagc gtcgagtttt tcgaatcgct
     3841 cgccaggttc cagcccttcc gctaaagcga ccagcagatt agtgatacca gcagaagccg
     3901 agaggactac taaacgcacg ttggcatcag aaagcacaat atcagcgctg cggttcatgg
     3961 cgtcaaaatc agctacgctg gtaccgccaa atttggagac aacaatttca gacataacta
     4021 cctcgtgtca ggggatccat tttcagcctt ggcacaaggg aagagcggaa gacgggtggg
     4081 cgcagagcga tacttcgcta ctattttcac ccagaagtgc tccaccactt gcgaaacgcc
     4141 cgactgcgaa cgcttctggt gacaacccag gggattcagc ccctgtagcc gatgatgaac
     4201 gtggccagcc gttcaatcac ctcggcgatg caccccctca ggtgttatca caggactggc
     4261 tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc ctgcgctagc gcaggtagta
     4321 catttataaa taaagggtga gcggggcggt tgtcaacgac ggggtcatgc ggatttttca
     4381 tccactcctg gcggtcagta gttcagctaa taaatgcttc actgcgctaa gggtttacac
     4441 tcaacattac actaacggca ctaaaaccat cacatttttc tgtgactggc gctacaatct
     4501 tccaaagtca caattctcaa aatcagaaga gtattgctaa tgaaaaacat caatccaacg
     4561 cagaccgctg cctggcaggc actacagaaa cacttcgatg aaatgaaaga cgttacgatc
     4621 gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt tctccgcaac cttcgacgat
     4681 cagatgctgg tggattactc caaaaaccgc atcactgaag agacgctggc gaaattacag
     4741 ggtctggcga aagagtgcga tctggcgggc gcgattaagt cgatgttctc tggcgagaag
     4801 atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc tgcgtaatcg tagcaatacc
     4861 ccgattctgg ttgatggcaa agatgttatg ccagaagtca acgcggtgct ggagaagatg
     4921 aaaaccttct cggaagcgat tatctccggt gagtggaagg gttataccgg caaagcaatc
     4981 actgacgtag tgaacatcgg gatcggcggt tctgacctcg gcccgtacat ggtgaccgaa
     5041 gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg tttctaacgt cgatgggact
     5101 cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca cgctgttcct ggtagcatct
     5161 aaaactttca ccactcagga aaccatgacc aacgcccata gcgcgcgtga ctggttcctg
     5221 aaagcggcag gtgatgaaaa acacgttgcg aaacattttg cggcgctctc aaccaatgcc
     5281 aaagccgttg gcgagtttgg tattgatact gccaacatgt tcgagttctg ggactgggtc
     5341 ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga ttgttctctc catcggcttt
     5401 gataacttcg ttgaactgct ttctggcgca cacgcaatgg acaagcattt ctccaccacg
     5461 cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg gcatctggta caacaatttc
     5521 tttggtgcgg aaactgaagc gattctgccg tatgaccagt atatgcaccg tttcgcggcg
     5581 tacttccagc agggcaatat ggagtccaac ggtaagtatg ttgaccgtaa cggtaacgtt
     5641 gtggattacc agactggccc gattatctgg ggtgaaccag gcactaacgg tcagcacgcg
     5701 ttctaccagc tgatccacca gggaaccaaa atggtaccgt gcgatttcat cgccccggct
     5761 atcacccata acccgctctc tgatcaccac cagaaactgc tgtctaactt cttcgcccag
     5821 accgaagcgc tggcgtttgg taaatcccgc gaagtggttg agcaggaata tcgcgatcag
     5881 ggtaaagatc cggcaacgct tgactacgtg gtgccgttca aagtgttcga aggtaaccgc
     5941 ccgaccaact ccatcctgct gcgcgaaatc actccgttca gcctgggtgc gttgattgcg
     6001 ctgtatgagc acaaaatctt tactcagggc gtgatcctga acatcttcac cttcgaccag
     6061 tggggcgtgg agctgggtaa acaactggca aaccgtattc tgccagagct gaaagatgat
     6121 aaagaaatca gcagccacga tagctcgacc aatggtctga ttaaccgcta taaagcgtgg
     6181 cgcggttaat catcatgcgt gcctgatgcc gatatgtagg ccggataagg cgtttacgcc
     6241 gcatccggca accggagcct gatgccaata tcgacattaa tgcatagtat gaggagttaa
     6301 ataaccttaa aaacaatgcc gacaataagt cggcattgtt ttatcagata aatccccttg
     6361 tctgtgattt aacggaaatc ataccgtgag gttaatccta aaatagattt ttaatcgttg
     6421 tttatttcgg agaatacgca gattaattac ttttgttttt attttaattt tatgattttt
     6481 attgttattt aaatataagt tgaaacttat atttgatatt cattccaatt atcctaaaac
     6541 tccatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac tcgatcccgc
     6601 ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt atgaaaaaag
     6661 ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg gctgcacctg
     6721 tacaggtggg cgaagcggca gagtcggcag caacgtcggt ttcggcgggg agttcctccg
     6781 cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca accggtggcg
     6841 gtgatggttc taataccggg accaccacaa ccacaaccac gaccaccagt acccagtaat
     6901 aaagtatgta tccccaaaat aatttgagtc attgcatctg tggcttgaag tatgaaggga
     6961 tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag tcgtgaagcg
     7021 acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct
     7081 gggcaattca ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga
     7141 tattcaaaat atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt
     7201 tgtggtgctg gccttcgctg aagacggaca acaaaaatgg gtcactcagg atcaagccac
     7261 tcttgtcaca caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga
     7321 agtgaataac cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac
     7381 atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc
     7441 agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa ccctggttcg
     7501 cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct actggatcga
     7561 cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa
     7621 aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc gttgcttttg
     7681 agtgtgggag cgtcatcggt cttcgcggca ggaaccgtca aggtgttcag caatggcagc
     7741 agtgaggcca aaacgctaac gggcgcagag catttaatcg atctggtagg gcaacctcga
     7801 ctggcaaaca gctggtggcc tggtgcggtg attagcgaag agctggcaac ggcggcagca
     7861 ttgcgtcagc agcaggcgtt gctgacacgg ctggcagaac agggcgcaga ttccagcacc
     7921 gacgatgccg ccgccattaa cgccttacgc cagcaaatcc aggcattgaa agtgacaggc
     7981 aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg
     8041 ttgcagggca actacacgct gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg
     8101 cttatcagcc atcctggcaa tcagccattc actccaggtc gcgacgtggc gagctatctc
     8161 tctgaccaga gcctgctcag cggtgcggat cgcagctacg cgtgggtcgt ttacccggac
     8221 ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga gccgatgccc
     8281 ggcagcatta tttatgttgg cctcgcagac tccgtctgga gtgagacgcc tgatgccctt
     8341 aacgccgaca ttcttcagac tctgacgcag cgggtacccc aataatgaaa aaaagacatc
     8401 tgcttagctt actggcgctg ggcattagca cagcttgcta cggcgaaaca tatcctgcgc
     8461 ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt attacaaact cccaccgcgc
     8521 gtatggcacg ggaaggggag ttgagtctga actatcgcga taacgatcag taccgttatt
     8581 actcagcttc agtgcaactc ttcccgtggc tggaaacaac gctgcgctac accgacgtgc
     8641 gcacccggca gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat aaagataaag
     8701 ccttcgatct caaactgcgt ttgtgggaag agagttactg gctgccgcaa gtggcggttg
     8761 gtgcgcggga tatcggcggt acggggctgt ttgatgcgga atatcttgtt gccagcaaag
     8821 cctgggggcc gttcgatttt acgctcggcc tgggctgggg atatctgggc accagcggta
     8881 atgtaaaaaa tccgctctgt tcagccagtg ataaatattg ctatcgcgat aacagctaca
     8941 aacaggcggg atctatcgac ggtagccaga tgttccacgg tcctgcctca ctgtttggcg
     9001 gcgtggaata ccagacgccc tggcaaccgc tgcgtctgaa actggagtat gaaggtaata
     9061 attatcagca ggattttgcc gggaagctgg agcaaaaaag taagtttaac gtcggggcga
     9121 tttatcgtgt taccgattgg gccgacgtta accttagcta tgaacgtggc aacaccttta
     9181 tgtttggcgt cacgctgcgc accaacttta acgatctgcg cccgtcctac atcgataacg
     9241 cccgcccgca atatcaaccg cagccgcagg atgccatttt gcagcattcg gtggtggcga
     9301 atcagttaac gttgctgaaa tacaacgccg gacttgccga tccgcagatc caggcgaaag
     9361 gcgatacgct gtatgttacc ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca
     9421 tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg atccgcatta
     9481 cggaaaatcg ccttaacatg ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa
     9541 atcatctcgc cggagagccg ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc
     9601 cagtggttcc gaagtccacc gagcagggct ggtatatcga caaatcacgc tttgatttcc
     9661 atatcgatcc ggtgctgaac cagtcggtcg gtggcccgga aaacttttac atgtatcagc
     9721 tgggcgtgat gggaacggca gatttgtggc tgacggacca tctactgacc accggcagcc
     9781 tgtttgcaaa tcttgccaac aactacgaca agtttaacta caccaatcca ccgcaggact
     9841 cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat gtctatgtga
     9901 ataacctgca agccaactac ttccagcatc tgggcaacgg cttctacggt caggtctatg
     9961 gaggttatct cgaaaccatg tttggcggtg cgggggccga agtgttgtat cgtccgctgg
    10021 acagtaactg ggcgtttggt ctggatgcca actacgttaa acagcgcgac tggcgtagtg
    10081 caaaagatat gatgaaattc accgactaca gcgtgaaaac cggacatctg accgcctact
    10141 ggacgccatc tttcgcccag gacgtgttgg tgaaagccag cgtcgggcag tatctggcag
    10201 gggataaagg cggcacgctg gagatcgcta aacgctttga cagcggcgtg gtggtgggtg
    10261 gctatgccac gatcaccaac gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag
    10321 gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg cccgacacgc agccgtgcgg
    10381 cgattggctg gacgccgctg acgcgtgacg gtggtcagca acttgggcgt aagttccagt
    10441 tgtatgacat gaccagcgac cgtagcgtaa atttccgcta agtcatggga aaggtgccag
    10501 ttttcgcact cactggcacc ctcattctta atgacctgtt gagccatcag gaacgcagtt
    10561 cacccccggt ttacgatctg gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga
    10621 tatcggttgc aggtctggtt gcctgagcga ctgggccatc attgggctgg agacattgct
    10681 ttgcggtagg tcggctttat cagcagtacc gagcggacca gcataagcag gaagaacaga
    10741 gactgataac atcaaagcag caaaataagg cttcattttt accaccttta tcaggttacg
    10801 tttcatttgt tccagaggat cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca
    10861 cctgaatggg tgatttttga attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg
    10921 ccaggcgcgg cttatccggc ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc
    10981 aaatccaggt tgaacaaaac atacacaaaa aatatagatc tccgtcacat ttttgcgtta
    11041 tacaggaagc tcgccactgt gaaggaggta ctgctatgac gtcactctct cgtccgcgcg
    11101 tggagtttat ctccaccatt ttgcagaccg tactcaatct tggcctgctg tgcctcggcc
    11161 tgattttggt tgtcttcctc ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc
    11221 cagaacaaac cagcaaatat gagctggtag aaggactggt ggtttacttt ctctatttcg
    11281 aatttatcgc gctgattgtg aagtactttc agtccggttt tcacttcccg ctacgttact
    11341 ttgtctatat tgggatcacc gccattgtgc gcttgatcat cgtcgatcat aaatcgccac
    11401 tggatgtgtt gatctactcg gctgcgatcc tgttactggt gatcaccctg tggctgtgta
    11461 attcgaaacg gctgaagcgg gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg
    11521 acaggaagat tacagcgtag cagtttgttg tgttttcttc gtttccggtt cccagagcgc
    11581 ttccagctcc tcaagggttt tacctttggt ttccgggaca aatttccaca taaacagtgc
    11641 tgccagaacg cccatacaac cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac
    11701 cagccaggag tttttgtcca tcatcgggaa ggtccaggag acgaagtagt tcgccagcca
    11761 ctgggccgcc accgcgattg ccagcgcttt accacgaata gcattcggga agatttccga
    11821 cagcagtacc cagcataccg gaccccagga catggcaaag gcggcaacat agaacagcat
    11881 cgacagtagc gccacaatac ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat
    11941 accgattgcc attccgagtg cgccgataat ttgcagtggc ttacgaccaa atttatccac
    12001 agtcataatt gccagtacgg taaaggtgag gttgataact ccgacaataa tggtctgcaa
    12061 cagcgcgata tccgtgctgg cccccagcgt tttgaacact tccggcgcgt agtacagcac
    12121 cacattgatg ccgacaaatt gctggaagat ggagagcata acgccgatta caatcacgcc
    12181 cacgccaaac atcagcagac gaccaccggt tttgcggcca tgatccaggg agtgtttaat
    12241 ttcctgtact gcctgagttg caagcgtgtt gcccataatt ttgcgcagga tactttccgc
    12301 ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt tctggcacgg tatacagcag
    12361 cattaagaac agcagtgcag ggatacattc cgaggcaaac atataacgcc agccgtcagt
    12421 attcagccag ctggcatcac cggaacgggc aataaaatag tttacgcagt aaactaaaag
    12481 ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt tttccgcgaa tatgagctgg
    12541 agccagttcc gcaatataca ttggtgaaag cattgaggct aaaccaacgc caataccgcc
    12601 aataatacga taaataacaa attccgggac ataacctgcc agataaatag gtactgtgtt
    12661 gtccgggttt atagaggtaa aaccaagttc tggccaggca gaacctacac cagaaataaa
    12721 aaacaggaca gcagaaatct taagtgaatc acgacggccg aagcggttac tgcaataacc
    12781 accgagggca ccgccgatga tgcaaccaat cagagcgctg gctacgcaaa atcctaacag
    12841 ggagttggca gcggattcac ttaagttttg tggagcaaca aagacggtat tgagtgactc
    12901 aacagtaccg gaaataacgg cggtgtcgta gccaaataat aaaccaccta atgtagcgac
    12961 taaggtaatc gaaaatatat aactggaatt atactgggta ttcattcaga cctgccttag
    13021 accattctga tcttttatca gtgcattgat gttggaattg tcaggcagtt aaggcaatta
    13081 tcatttttta caatgcgttt acgtaatgtc ttttctgtga tcttaattgt gataattatc
    13141 aaaaattgat gaaaacaata ctattgccgt gactcagagc acgaaagaga attatcgtaa
    13201 gtgggaaaac aaataacgta aaaataataa gttctatgat gggaatatta accggcgaac
    13261 gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg agcgccgaat aatagtcaca
    13321 agttgggata acgtaagttg agggtgcagc ggcataacat tggcagaaca acatctttaa
    13381 cctttcacac cacctgccgt caggccgttg accagccagc gttgagccag caagaagacg
    13441 atggtgatcg gtaatgcaga catcacggca gcggcggcaa agtcacccca caggtagttt
    13501 tgcgggttga ggtattgctg catccccacg gctagggtgt agctgtttac gtcacgcagt
    13561 aacagcgacg cgaccggaac ttcagtaatg gcagcgataa acgacaggat gaataccacc
    13621 gccagaatcg gtactgacag cggcaacagg acaaggcgga aggcctgcca cggtgtcgca
    13681 ccatccagcg cagccgcttc ttccagcgaa ctgtcgatgg tttcgaaata gcctttgatg
    13741 gtccagacat gcagcgcaat cccacccaga tacgcgaaaa ttacgccgcc gtgagtattc
    13801 aggccaatga atggaatgta ctcacccaga cgatcaaaca acgcatacaa cgcgaccagt
    13861 gaaagtactg ccgggaacat ctggaaaatc agcattcctt tcagcagcgt cgctttgcct
    13921 ggaaagcgca tacgggcgaa agcgtaggcg caggtggtgg agagcgccac aatgccaatc
    13981 gcggaaatcc cagcgacctt taccgagttc cacagccaca gcagtaccgg gaatggcggt
    14041 ggcgtaatgc gaccatcagc ctgttcaacg ctaaaaccta acgccagttt ccagtgatcc
    14101 caggagattt gctccgggat caggctaccg gtcgcgaagt ttccctgacg cagcgagata
    14161 gcgacgacca tcagcagcgg gaacataatc gccgcgataa aaagtagcag tagcaggtga
    14221 gtaataaaca aacgtgcttt ttgcgatttc ggttggacca ttgccatttt tgttatctcc
    14281 cttaatcaaa cttcattcgc gtggctttca ggttcactat cgccagcgca cccaccagca
    14341 ggaagatcag cgtggcaatt gctgccgcca gaccgaagtc ctgacccccg ccgccttcaa
    14401 aagcgatacg gtaggtgtag ttaacaagca ggtcggtata accggctggc gtggtcgtgc
    14461 caagacgatc cgggccgccg ttggttaaca gctgaatcag cacgaagttg ttaaagttaa
    14521 aggcgaagct ggcgatcatc agcggcgtca gcggtttaat cagcagcggc agcgtaatct
    14581 taaagaagtt ctggaacgga cctgcgccat ccattgctga ggcttcatac aaatcgtccg
    14641 gaatcgcttt cagcaagccc atgcagagga tcatcatgta cggataaccc agccaggtat
    14701 tgacgataat cagcatcgtg cgagcggtgg tcggatcgct gaaccaggcg ggcttcacgc
    14761 caaacagcgc gctcaacatc atgttgattt caccgaagct ctggttaaac aatcctttga
    14821 aaatcaagat tgaaatgaac gatggcaccg cgtagggcag aatcagcagg acgcgataga
    14881 ccgctttgcc gcgcaacgct tcccactgca ccagacatgc cagaaccatg ccgaccgcca
    14941 ccgttaaaaa gacagtgatc agcgagaaca ccacggtcca gacgaaaatg gcgaggaacg
    15001 gtttctgaat gccttcgtcg gtaaagacgc gggtaaagtt tttccagccg gtggtcacgg
    15061 tgtaaccggg gcttagcttt tcatcccccc agttgccgtc ggcggtaatg gactggtaaa
    15121 agccaatttg gttattcgga cgatatttca cgccactctg attattcgtc aacgtgccgt
    15181 caccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg gcgcagggag ctcatcatca
    15241 ctttgttgcc atccggcaga atggcggtaa tgtcactcag cgcctgacga ttctgggtaa
    15301 tcacgcgcag attcgcgcgt tcgccttcgg gctgggcggt cgtttctttc agttgcagtt
    15361 tttgctcgcc gccaaattta aacgcgtcgg agaggtaatt tttgccggtt tcgccgtcgc
    15421 tgagcgccag ttgccactca tcgcccgccg ggtaaagacc aaagttatag gttttgcctg
    15481 cttgccagga gcgatctaac aacacttcct gcgcacgttc aaaagtcagc tggttagtgc
    15541 tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag agggaagagg acgaataatc
    15601 ccattccagc cattcccggg taaacatagc gccaggcgta ggctttacga ttggcgaaaa
    15661 tatacagccc cgctgaactc aatatcagcg tggtaatggc gaacaggtat tccccttgtg
    15721 cgtacattaa aacaacaagg taacccacca gcaggccgag cagacctagc actgaccatt
    15781 tcagcgcgtc gctttgccac caatgtttct ttttaatgac atccatgggg ttcttcctca
    15841 ttccaggacg gataaggctt tcacgcctta tccgacaaca actgcctgat gcgacactga
    15901 tgcgtcttat caggcctaca tgcgtttcag ttttgtaggc cggacaaggc gttcacgccg
    15961 catccggcat ttcacagcat tacttggtga tacgagtctg cgcgtctttc agggcttcat
    16021 cgacagtctg acgaccgctg gcggcgttga tcaccgcagt acgtacggca taccagaaag
    16081 cggacatctg cgggatgttc ggcatgattt cacctttctg ggcgttttcc atggtggcgg
    16141 caatacgtgg atctttcgcc aactcttcct cgtaagactt cagcgctacg gcacccagcg
    16201 gtttgtcttt attaaccgct tccagacctt catcagtcag cagatagttt tcgaggaact
    16261 ctttcgccag ctctttgttc ggactggcgg cgttaatacc ggcgctcagc acgccaacga
    16321 acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa ttcactttgc
    16381 tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt tcgcctttat
    16441 taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg tttttaatca
    16501 ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc acgtctttaa
    16561 tgtcgtactt gccgttttca tacttaaacg cataaccccc gtcagcagca atcagcggcc
    16621 aggtgaagta cggttcttgc aggttgaaca tcagcgcgct cttacctttc gctttcagtt
    16681 ctttatccag cgccgggatc tcttcccagg tttttggcgg gttcggcagc agatctttgt
    16741 tataaatcag cgataacgct tcaacagcga tcgggtaagc aatcagcttg ccgttgtaac
    16801 gtacggcatc ccatgtaaac ggatacagct tgtcctggaa cgctttgtcc ggggtgattt
    16861 cagccaacag gccagattga gcgtagccac caaagcggtc gtgcgcccag aagataatgt
    16921 cagggccatc gccagttgcc gcaacctgag ggaatttctc ttccagttta tccggatgct
    16981 caacggtgac tttaattccg gtatctttct cgaatttctt accgacttca gcgagaccgt
    17041 tatagccttt atcgccgtta atccagatta ccagtttacc ttcttcgatt ttggcgagag
    17101 ccgaggcgga aaacatcatc gtcgttaatg cggataatgc gaggatgcgt gcacctgttt
    17161 ttattttcat aatctatggt ccttgttggt gaagtgctcg tgaaaacacc taaacggact
    17221 ctagtttctt tatacggcaa cctctttcca tcctccttgc ccctacgccc caccgttgct
    17281 ttgtgtgatc tctgttacag aattggcggt aatgtgggga tgcgcacata aaatcgccac
    17341 gatttttgca agcaacatca cgaaattcct tacatgacct cggtttagtt cacagaagcc
    17401 gcgttctcat cctcccgcct cctcccccat aaaaaagcca gggggtggag gatttaagcc
    17461 atctcctgat gacgcatagt cagcccatca tgaatgttgc tgtcgatgac aggttgttac
    17521 aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt aacgaaagcc tggggcgagg
    17581 tcgtggtatc gaaagatatc aatctcgata tccatgaagg tgaattcgtg gtgtttgtcg
    17641 gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat tgccgggctt gagacgatca
    17701 ccagcggcga cctgttcatc ggtgagaaac ggatgaatga cactccgcca gcagaacgcg
    17761 gcgttggtat ggtgtttcag tcttacgcgc tctatcccca cctgtcagta gcagaaaaca
    17821 tgtcatttgg cctgaaactg gctggcgcaa aaaaagaggt gattaaccaa cgcgtcaacc
    17881 aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg caaaccgaaa gcgctctccg
    17941 gtggtcagcg tcagcgcgtg gcgattggcc gtacgctggt ggccgagcca agcgtatttt
    18001 tgctcgatga accgctctcc aacctcgatg ctgcactgcg tgtgcaaatg cgtatcgaaa
    18061 tctcccgtct gcataaacgc ctgggccgca caatgattta cgtcacccac gatcaggtcg
    18121 aagcgatgac gctggccgac aaaatcgtgg tgctggacgc cggtcgcgtg gcacaggttg
    18181 ggaaaccgct ggaactgtac cactatccgg cagaccgttt tgtcgccgga tttatcggtt
    18241 cgccaaagat gaacttcctg ccggtaaaag tgaccgccac cgcaatcgat caagtgcagg
    18301 tggagctgcc gatgccaaat cgtcaacaag tctggctgcc tgttgaaagc cgtgatgtcc
    18361 aggttggagc caatatgtcg ctgggtattc gcccggaaca tctactgccg agtgatatcg
    18421 ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca actcggcaac gaaacccaaa
    18481 tccatatcca gatcccttcc attcgtcaaa acctggtgta ccgccagaac gacgtggtgt
    18541 tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc agagcgttgc catctgttcc
    18601 gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc gggcgtttaa gcaccccaca
    18661 aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa agcaatgact caggagatag
    18721 aatgatgatt actctgcgca aacttcctct ggcggttgcc gtcgcagcgg gcgtaatgtc
    18781 tgctcaggca atggctgttg atttccacgg ctatgcacgt tccggtattg gctggacagg
    18841 tagcggcggt gaacaacagt gtttccagac taccggtgct caaagtaaat accgtcttgg
    18901 caacgaatgt gaaacttatg ctgaattaaa attgggtcag gaagtgtgga aagagggcga
    18961 taagagcttc tatttcgaca ctaacgtggc ctattccgtc gcgcaacaga atgactggga
    19021 agctaccgat ccggccttcc gtgaagcaaa cgtgcaggga aaaaacctga tcgaatggct
    19081 gccaggttcc accatctggg caggtaagcg cttctaccaa cgtcatgacg ttcatatgat
    19141 cgacttctac tactgggata tttctggtcc tggtgccggt ctggaaaaca tcgatgttgg
    19201 cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa gctggtggtt cttcctcttt
    19261 tgccagcaac aatatttatg actataccaa cgaaaccgcg aacgacgttt tcgatgtgcg
    19321 tttagcgcag atggaaatca acccgggcgg cacattagaa ctgggtgtcg actacggtcg
    19381 tgccaacctg cgtgataact atcgtctggt tgatggcgca tcgaaagacg gctggttatt
    19441 cactgctgaa catactcaga gtgtcctgaa gggctttaac aagtttgttg ttcagtacgc
    19501 tactgactcg atgacctcgc agggtaaagg tctgtcgcag ggttctggcg tcgcgtttga
    19561 taacgaaaaa tttgcctaca atatcaacaa caacggtcac atgctgcgta tcctcgacca
    19621 cggtgcgatc tccatgggcg acaactggga catgatgtac gtgggtatgt accaggatat
    19681 caactgggat aacgacaacg gcaccaagtg gtggaccgtt ggtattcgcc cgatgtacaa
    19741 gtggacgcca atcatgagca ccgtgatgga aatcggctac gacaacgtcg aatcccagcg
    19801 caccggcgac aagaacaatc agtacaaaat taccctcgca caacaatggc aggctggcga
    19861 cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc tacgccaagt gggatgagaa
    19921 atggggttac gactacaacg gcgatagcaa ggttaacccg aactacggca aagccgttcc
    19981 tgctgatttc aacggcggca gcttcggtcg tggcgacagc gacgagtgga ccttcggtgc
    20041 ccagatggaa atctggtggt aatagcaaaa cctgggccgg ataaggcgtt tacgccgcat
    20101 ccggcataaa aacaggttgt cattatctga aaggggcgaa agcccctctg attatcgggt
    20161 ttagcgcgct attgcctggc taccgctgag ctccagattt tgaggtgaaa acaatgaaaa
    20221 tgaataaaag tctcatcgcc ctctgtttat cagcagggtt actggcaagc gcgcctggaa
    20281 ttagccttgc cgatgttaac tacgtaccgc aaaacaccag cgacgcgcca gccattccat
    20341 ctgccgcgct gcaacaactc acctggacac cggtcgatca atctaaaacc cagaccaccc
    20401 aactggcgac cggcggccaa caactgaacg ttcccggcat cagtggtccg gttgctgcgt
    20461 acagcgtccc ggcaaacatt ggcgaactga ccctgacgct gaccagcgaa gtgaacaaac
    20521 aaaccagcgt ttttgcgccg aacgtgctga ttcttgatca gaacatgacc ccatcagcct
    20581 tcttccccag cagttatttc acctaccagg aaccaggcgt gatgagtgca gatcggctgg
    20641 aaggcgttat gcgcctgaca ccggcgttgg ggcagcaaaa actttatgtt ctggtcttta
    20701 ccacggaaaa agatctccag cagacgaccc aactgctcga cccggctaaa gcctatgcca
    20761 aaggcgtcgg taactcgatc ccggatattc ccgatccggt tgctcgtcat accagcgatg
    20821 gcttactgaa actgaaagtc aaaacgaact ccagctccag cgtgttggta ggaccactat
    20881 ttggttcttc cgctccagct ccggttacgg taggcaacac ggcggcacca gctgtggctg
    20941 cacctgctcc ggtgccggtg aagaaaagcg agccgatgct caacgacacg gaaagttatt
    21001 ttaataccgc gatcaaaaac gctgtcgcga aaggtgatgt tgataaggcg ttaaaactgc
    21061 ttgatgaagc tgaacgcctg ggatcgacat ctgcccgttc cacctttatc agcagtgtaa
    21121 aaggcaaggg gtaattacgc cccacagtgc tgattttgca acaactggtg cgtctcctgg
    21181 cgcacctttt tttatgcttc cttcctggga tatgagcgat tttttatagt acttcacttc
    21241 ttcttcacta agaatgtcca ttatttcaat gcattatcag agattctttt cctttcgccg
    21301 gtagtgtctg gacgctcagg ctacttttcc aggttatttt atttctgtat tacagaggtt
    21361 ttatgatagg tcatatcaca aattctggcg gcaataactc tttgatgaaa catgatgtgg
    21421 tgcaaggaaa taatatagta gatcttgatt tactacgtaa tttcaatggg gtgacaggtt
    21481 taaacagaga taactttatt tatatcagtg atatattttt aaatataaaa caacggaacg
    21541 aaaaaaatca tgcaataaat atgttacgtg aagtttcaat tagtaatgat actataagtg
    21601 taaaattcta cagaaatgaa aaaatagaat gcgcttgtaa ttttctgatg gataaagatg
    21661 cgcaggggta tatcgacctg tctgattttg atttaacaaa ttgtcatttt aaaggtgacg
    21721 ttatttctaa ggtgt
//
LOCUS       DADKXM010000056        21468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.056, whole genome shotgun sequence.
ACCESSION   DADKXM010000056 DADKXM010000000
VERSION     DADKXM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.056"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..446)
                     /locus_tag="JQR05_002143"
     CDS             complement(<1..446)
                     /locus_tag="JQR05_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY5324316.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /locus_tag="JQR05_002144"
     CDS             complement(501..1352)
                     /locus_tag="JQR05_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY5324317.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /locus_tag="JQR05_002145"
     CDS             complement(1365..2165)
                     /locus_tag="JQR05_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY5324318.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JQR05_002146"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JQR05_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY5324319.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPAEKAAPAPAAAAPKAAPTPAKPMWPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JQR05_002147"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JQR05_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY5324320.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="JQR05_002148"
     CDS             complement(5216..6814)
                     /locus_tag="JQR05_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5324321.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LVQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="JQR05_002149"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="JQR05_002149"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY5324322.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDIYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="JQR05_002150"
     CDS             complement(8493..9071)
                     /locus_tag="JQR05_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY5324323.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="JQR05_002151"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="JQR05_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY5324324.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="JQR05_002152"
     CDS             10510..10689
                     /locus_tag="JQR05_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY5324325.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="JQR05_002153"
     CDS             complement(10809..11168)
                     /locus_tag="JQR05_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY5324326.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="JQR05_002154"
     CDS             complement(11530..11874)
                     /locus_tag="JQR05_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY5324327.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="JQR05_002155"
     CDS             12006..13916
                     /locus_tag="JQR05_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY5324328.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQRALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="JQR05_002156"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="JQR05_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY5324329.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="JQR05_002157"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="JQR05_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY5324330.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTLQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15495..17180
                     /gene="fadD"
                     /locus_tag="JQR05_002158"
     CDS             15495..17180
                     /gene="fadD"
                     /locus_tag="JQR05_002158"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY5324331.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDILGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17262..18377
                     /gene="rnd"
                     /locus_tag="JQR05_002159"
     CDS             17262..18377
                     /gene="rnd"
                     /locus_tag="JQR05_002159"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY5324332.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="JQR05_002160"
     CDS             complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="JQR05_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY5324333.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19452..20054)
                     /locus_tag="JQR05_002161"
     CDS             complement(19452..20054)
                     /locus_tag="JQR05_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY5324334.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(20411..20977)
                     /locus_tag="JQR05_002162"
     CDS             complement(20411..20977)
                     /locus_tag="JQR05_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5324335.1"
                     /translation="MPSEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNTQENKNPQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     EILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21189..21416
                     /locus_tag="JQR05_002163"
     CDS             21189..21416
                     /locus_tag="JQR05_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5324336.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         5327 a         5330 c         5506 g         5305 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 acgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gctacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc acattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcagc cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtactttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg caccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttc gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaatgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggt tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgggtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagcgaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcgg gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgacgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga cgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt cccacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gccaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccct gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15361 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15421 ttaatattat gttaacagca tatatatcat ttggggttgc gatgacgacg aacacgcatt
    15481 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15541 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15601 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15661 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15721 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15781 gtgccgggat gatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15841 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacactctgg
    15901 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15961 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgtttggtgc
    16021 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    16081 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    16141 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    16201 aacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    16261 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16321 gtgggcagaa cctgcttatc actaacccgc gtgatattct agggttggta aaagagttag
    16381 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16441 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16501 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16561 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16621 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16681 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16741 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16801 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16861 acatgattct ggtttccggt tttaacgtct atcccaacga gattgaagat gtcgtcatgc
    16921 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16981 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    17041 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    17101 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    17161 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    17221 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    17281 cgctggcttc tttgtgtgaa gccgtccgtg cctttccggc gatagccctg gatactgaat
    17341 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17401 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17461 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17521 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17581 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17641 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17701 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17761 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17821 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17881 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17941 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    18001 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    18061 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    18121 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    18181 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    18241 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18301 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18361 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18421 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18481 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18541 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18601 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18661 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18721 caaattgctc aaagtgcagc gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18781 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18841 ccaataatcg agctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18901 cagcctgcgg atgctggact agctcatcac gaaatgcgca actttctgga tttcgcgagc
    18961 agtaatgtag ctgccagtcg acgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    19021 gggtgatacc aataccgccc gcgataaaca gatgctttct ggctgaggga attagcgcaa
    19081 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    19141 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    19201 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    19261 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19321 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19381 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19441 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19501 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19561 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19621 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19681 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19741 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19801 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19861 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19921 aaacctggat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19981 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    20041 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    20101 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    20161 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    20221 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    20281 aaaaacgttc aaaaccgata acaaccatgc tgtaaccaaa tgactcatat cacaaatcag
    20341 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20401 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20461 cttcctgttc ataaatacca tactttgaca aaatctcttt acgagtatct gttggtaatg
    20521 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20581 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20641 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20701 ttttcaatgt gctgcggtca ggttgaggat ttttattctc ttgagtattt ttgtgctcaa
    20761 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20821 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20881 catcagaaag tccgcactta gctgcaaacg cccggcttga ttctccattc attgcctctt
    20941 caaggcgttc cttgaaacgc attatttccg agggcataaa aatgcgctct gcatttttgc
    21001 tttctttttc acctgtcatg ctcaatctct ctgttatata aagaatttta aagaattctt
    21061 tgatttgcat tggttaaaat aaaaatgcgc atagctcttg aaatgcgcat ttttctgcgc
    21121 tatcgttttt cccgatagcg gtagccggta tcagtaaccg aaaacgttaa gagagtaagg
    21181 atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc
    21241 gtatgaaggg gaagacacta tctcaattat ccagagaggc aggcttaaag cctgacacta
    21301 tgcgtaacgt atttcgctgc catataccac gatacgaagc aatcattgct agatatttgg
    21361 atatggaacc agcccagatt tggccttctc ggtatatgga gaaaacatca tgttaaccca
    21421 ttgggtaaca gcgcaagaat gcgtaggaat gtcatgcttt cctaaaca
//
LOCUS       DADKXM010000057        21407 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.057, whole genome shotgun sequence.
ACCESSION   DADKXM010000057 DADKXM010000000
VERSION     DADKXM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.057"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JQR05_002164"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JQR05_002164"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY5324337.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="JQR05_002165"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="JQR05_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY5324338.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="JQR05_002166"
     CDS             complement(2650..3201)
                     /locus_tag="JQR05_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY5324339.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="JQR05_002167"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="JQR05_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY5324340.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5216
                     /gene="cybC"
                     /locus_tag="JQR05_002168"
     CDS             4830..5216
                     /gene="cybC"
                     /locus_tag="JQR05_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY5324341.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLADNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="JQR05_002169"
     CDS             complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="JQR05_002169"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY5324342.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="JQR05_002170"
     CDS             complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="JQR05_002170"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY5324343.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8415..10070)
                     /gene="treC"
                     /locus_tag="JQR05_002171"
     CDS             complement(8415..10070)
                     /gene="treC"
                     /locus_tag="JQR05_002171"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY5324344.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPCFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNGAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10120..11541)
                     /gene="treB"
                     /locus_tag="JQR05_002172"
                     /pseudo
     CDS             complement(10120..11541)
                     /gene="treB"
                     /locus_tag="JQR05_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11660..12607)
                     /gene="treR"
                     /locus_tag="JQR05_002173"
     CDS             complement(11660..12607)
                     /gene="treR"
                     /locus_tag="JQR05_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY5324345.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12792..12845
                     /gene="mgtL"
                     /locus_tag="JQR05_002174"
     CDS             12792..12845
                     /gene="mgtL"
                     /locus_tag="JQR05_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY5324346.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12986..15682
                     /gene="mgtA"
                     /locus_tag="JQR05_002175"
     CDS             12986..15682
                     /gene="mgtA"
                     /locus_tag="JQR05_002175"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY5324347.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGVNTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKNLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="JQR05_002176"
     CDS             complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="JQR05_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY5324348.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="JQR05_002177"
     CDS             complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="JQR05_002177"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY5324349.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="JQR05_002178"
     CDS             complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="JQR05_002178"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY5324350.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="JQR05_002179"
     CDS             complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="JQR05_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY5324351.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18175..18570)
                     /locus_tag="JQR05_002180"
     CDS             complement(18175..18570)
                     /locus_tag="JQR05_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY5324352.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="JQR05_002181"
     CDS             complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="JQR05_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5324353.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19486..20079
                     /locus_tag="JQR05_002182"
     CDS             19486..20079
                     /locus_tag="JQR05_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5324354.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20224..20676
                     /locus_tag="JQR05_002183"
     CDS             20224..20676
                     /locus_tag="JQR05_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY5324355.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20799..21407
                     /locus_tag="JQR05_002184"
                     /pseudo
     CDS             20799..21407
                     /locus_tag="JQR05_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         5159 a         5320 c         5640 g         5288 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgccgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgct gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgcagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa gaagcgcagg
     5161 ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat cgttaattcc
     5221 tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa tgatgcacca
     5281 cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc acaaatttgc
     5341 cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg cgcagcgttg agttcgtcga
     5401 gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg atgcgtttta
     5461 ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg gagagggaaa
     5521 tcccctggcg tttgatacga gtgtcattca gatctttaat gatcttgtct tccattgctt
     5581 tggtaaatgg ctgaccagaa tttacccgcc atgtgctttt gttatagcaa ccggggcatt
     5641 catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg acgatgtcga
     5701 caggatagta ctgatgataa ttcattattt cgcctggctt atcagtccgc gtgtaggctg
     5761 catccggtaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta cagccgttgc
     5821 caaatgtagg ccggataagg cgtttacgcc gcatccggca tttgcttagc gcagagtgaa
     5881 gattaaccta tctgcccatt ccccaaatgt ttaacgcggc gcttaacttc ttcctgctta
     5941 ccagcgttaa acggacgtgc atccgggcta cctaaatatc cgcacacgcg gcgagttacc
     6001 gacacacggg aggcgtcatg gttaccacat ttcgggcaag tgaagccttt gctggtgcac
     6061 tcgaactcac cggtaaagcc acactcgtag cactcatcaa tcggtgtatt ggtgccgtaa
     6121 tacggtacat gctgatagct gtaatcccag acatcttcca gcgctttcag gttgtgctga
     6181 atgtttggat actcgccgta gcaaatgaaa ccaccgttcg ccagcggcgg gtaaggcgct
     6241 tcaaagtcga tcttgtcgta cgggttcacc ttcttctcca catcaaggtg gaaactgttg
     6301 gtgtagtaac ctttatcggt cacgcccggc accacgccaa actcagcagt atcgagacgg
     6361 cagaagcggt cgcacaggtt ttcactcggc gtgctgtaga gactgaaacc ataacccgtt
     6421 tcttctttcc actgatccac tgcctgacgc agacgttcaa caatggcgat acctttcgcg
     6481 cgaagcttct cgttgtcgta aacatgctcg ccgccgaaca gcgcgttaat ggtttcgtgg
     6541 atgccgatgt aacccagcga aatagacgca cgaccgtttt tgaagatttc agaaacatca
     6601 tcgtcagcat tcagacgtac gccacaagca ccttccatat agaggatcgg ggcgacgcgc
     6661 gctttcacgc cttcgagacg agcgatacgg gtcatcagtg ccttacgtgc cagcaccaga
     6721 cgttcatcca gcagcttcca gaaggtggct tcatcgcctt ttgcttccag agcaatacgc
     6781 ggcaggttca ggctgatcac gccgaggttg ttacgaccat cgtggatctg ctcgccgttt
     6841 tcattttccc acacgccgag gaagctgcgg cagcccatcg gggttttaaa cgaaccggtg
     6901 actttcacta cctgatcgta gttcaggata tccggataca tgcgcttgct tgcgcactcc
     6961 agcgccagct gtttgatgtc gtagttcgga tcgccttttt tatggttcag gccatcgcga
     7021 atcgcaaaca ccagtttcgg gaacaccgca gttttacggt ttttaccgag gcctgcgata
     7081 cggttacgca ggatcgattc ctgaatcagg cgcgattccc agctggtgcc cagaccaaaa
     7141 ccaaaggtta caaacggcgt ctgaccgttg gcggtgtgca gggtgtttac ttcgtactcc
     7201 agcgactgga aggcatcgta gcactctttg atggtacgag agttagcgta gccttcggcg
     7261 tccgggatgt tccactcttc tgcggtcttg cgatgtttgt tgtagctggc ggtaacaaac
     7321 ggcgccagca cttcatcgat acggttaatg gtggtgccgc cataaatatg gctggcgacc
     7381 tgagcaataa tctgcgcagt taccgcggtt gccgtagaga tcgacttcgg cggttcaatc
     7441 tcggcgttcc ccattttaaa gccctgggtc agcatgcctt tcaggtcgat caacatgcag
     7501 ttgaacatcg ggaagaacgg cgagtaatcg agatcgtgat agtgaatatc gccacgctca
     7561 tgtgcctgca ccacgtcacg cggcagcagg tgctggcgtg catagtgttt agccacgatc
     7621 ccggccagca ggtcgcgctg ggttggaatc accttgctgt ctttgttggc gttttcgttg
     7681 agtaacgagg cgttggtctg ctcgaccaga ccacggatct cctggttcag gcgaccgcgt
     7741 ttttcacgtt caatgtcgcg atcgtgacgg tactcgatgt aagcacgagc cagttgtttg
     7801 tatggacccg acatcagctg gttttcaact gcggtctgga tctcgttgat atccacctgg
     7861 ttgcggccct gcatctgctc gctgacaacc gcggcaacag tggcgcaata atcggcatca
     7921 tcgacttccg ctgctttagc tgcacgcaga atcgcttctt tgatgcgctc tgatttaaac
     7981 ggcactttgc agccgtctcg tttcatcaca tgcggtgtca tgatcgctcc atatttttaa
     8041 gaacaggtta tccacagaaa ttgggaaagg cgttttccgg ttttttcgtt tcgttttccg
     8101 gtgctttccg caatcgccat ccgcttatcc acaacgccgc acctttttcg tggggagctg
     8161 tagtagcaat tatagtcgat taatacaaca tattgggttg ggacgcattt taaagtctat
     8221 atatagtgct ttgcatcaag gatgttttaa ctttttttga tgtagctcaa agtaaaaagc
     8281 agagcgtacg gatgacgggc gctacagcgt tatgtaaatt ttttaatgaa tttgctggtt
     8341 gaaaaatcaa caaaaacaac atgctgacag acaaaacccc ggaatgaccg gcattatcgg
     8401 ggcttaggga agatttactt ctgtaaccac cagacagcct caaaaggccg taaattcatg
     8461 gcacagggtt gtggtgaggc ttcttcgtag ttatgcatca caagctgcca gttgccgcgc
     8521 atttgccctg gctgccaggg ttggatctca cggctaaggt tggcaatgac cagcaaggtt
     8581 tgccccttcc attcacggcg atagcaccac aatacagggc tgtttggcag cagatcctgg
     8641 taattgcccc atgtcaggat ggcttcttgc ttacgcagtg cgattaactt ttggtaggtg
     8701 taaaacacag aggagtcatc ggccagcgcg gcttctacgt tgatttgttg atagttatcg
     8761 cctaggccaa tccacggttc gccagccgta aacccggcat tatcgccgtt gctccattgc
     8821 atgggcgtgc ggctgttgtc acgggattta ctggcgagga ttgccaataa ctcgtcggca
     8881 tcacgaccat cgttgcgcag ctcggcaaac atattgaggc tctccacgtc gcgatagtca
     8941 gtaatgcgcg tgaaatgcgg gttggtcatg ccaatctctt cgccctggta gatatacggc
     9001 gttccctgca tgccatgcag caccatcgcc agcatttttg ccgcaggcac gcggtattca
     9061 ccttcatcac caaagcgaga aacaatgcgc ggctgatcgt ggttacacca gaacaaggca
     9121 ttccatgccc cgttgtgcat tccttgttgc cagtggcgga acaatgtttt caacgccaca
     9181 aagtcaggtt tagccagcgt ccatttttca ccaccgggat aatcgacctt caggtgatga
     9241 aaattaaagg tcatcgacaa ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca
     9301 aggctggtgg aggacatttc acctacggtc attaacccgc gtggcgtaaa cacatcgcgg
     9361 ttcatctcgt gcaaaaactc gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg
     9421 ccatccaggt cttcagggaa acacgggtct ttggagatca gattcaccac atccaggcgc
     9481 aacccgtcga ccccacgatc ggcccagaat tcacagactt ttttcagctc tgcgcgtacc
     9541 gctggattct cccagttgag atccgcctgt tctggtgcaa agagatgcaa ataatactgt
     9601 tcgctttccg catgccagcg ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt
     9661 ggcgtttctg gttcgccatc gcgccagata taaaactggc ggtaagggct ttctttgttc
     9721 agcgcctcgc gaaaccaggc atgttgggta gaggtatggt taaacaccat atcgagaatg
     9781 atacgaatcc cgcgcgattt tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg
     9841 taggtgggat caatcgccgt atagttcgct acatcgtaac cgttatcgac ctggggagag
     9901 acataaaagg gggttagcca gatggcatca acgcccagtt tatgcagata gtccaggcgt
     9961 tggataacgc cacgtaaatc gccggtgccg ctacccgtgg tgtcctgaaa actctttgga
    10021 taaatctggt agataacgcc gttttgccac cagtggggaa gattagtcat tacgttattc
    10081 ctgcaaatgc gagggggcgc aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc
    10141 caggcggtat ttccgctgat agataaacga ggtgagtaca atcgggatga tgatggcgat
    10201 agccattgcc agcgcaaata cctgccagta gctcggttga atcgagagaa ttcccggcag
    10261 gccccctacg ccgataccat tcgccataac gccgttcagg ccgcatagca atcctgccag
    10321 accagaacca atcatcgcgc acagcatcgg gaagcgatat ttcaggttga tgccgtacat
    10381 tgcaggctca gtgaccccaa gccaggcgga gatagcggca ggcacggaga tctcgcgttc
    10441 attgtgcttg cggctggaaa tgataatgcc tatcacggcg gagccctgag cgatattcga
    10501 cagcgcaatc agcggccaca ctggcgtgcc acccatgctt tgaatcatct gcaaatcaat
    10561 agcaagggtg gtctggtgta cgccggtgat caccagcggc gcgtacagga agccaaacaa
    10621 tgccgcacca atcggagcaa agctgccggt catcaggtga cgtaccgcaa aggcaacgcc
    10681 atcgccaatc atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat
    10741 cagcgaacag acgggcacca ccaccagata gaggtaatcc ggtacgatgc gtttaaggcg
    10801 agtttcaata acgcccagcg ccagcccggc taacagtgcc gggatcacct gcgcctgata
    10861 gcccactttg gcgatgctga acatgccaaa gtcccacact tccggcagct gctgcccgag
    10921 cagataagcg ttcatcagct ggggggaaac cagtgtcacg ccaagcacga taccaaggat
    10981 cggcgtgccg cccatttttt tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa
    11041 aatcgcttca ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag ggtacatttg
    11101 cgccagcgtc tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa aaccgaggat
    11161 caaaccgccg ctaatcaacg cgggcagcaa cgggaagaag atctccgcga aatgagagat
    11221 caactgctca tgccatttca tattttgccg ggcggctttt tttacctgct ctttatcaac
    11281 ctgcgcctgt ccggttgacg ctattagtgc ttaatagtaa tcacccacgt tggtgccaat
    11341 caccacctga aattgcccgg cattggtgaa acagcctttc accatgggga gttgctcaat
    11401 ttctttcggt ctggcattgg ccggttggtt gaggacaaag cgcaggcgag taatacagtg
    11461 gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca atcaaccgat cgatatccgt
    11521 ttggtttatt ttgctcatca taaagcccca tggcagatga catttttggt tggctgcaga
    11581 atatattgcg cggaaaaatt taaaaacggg aacgttcccg aaacgcagcg aagatcacaa
    11641 tttatcgttc aggaaacgat caggacaggg tggcggggat gatgatttgt tgcggttcgc
    11701 tgcgcccggt tacctgtgcg atcaattggc aggccgcctg gcgtccagct tcggcgtagc
    11761 caggatctac ggtgacgatc tccggatgaa ggaatttcat taacggcgta ttacctacgc
    11821 tcgccagttg caaggtgtcg atgcgttgct cttgcaggta tttacttgcg ccaagagcca
    11881 gcgtgtcggt tgcgcacagt aacgcggtag tttcaggcgt aatcactttt gctacgtttt
    11941 catagccttg cttcatagca agccctggca gagcggcaac gggatgcagt ttatgcgctt
    12001 tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt cgtcacgtca ctatgcggca
    12061 cgccgagata actgatattg cgatgcccct ggtcatagag ccgttgcatc agaatcttga
    12121 ttgccccttc gtcgtcataa cagaccgaag caaagccttt tgcgtcacgc gccagcagaa
    12181 ccagcgatga ctgccagtgg gctaacattt cttcggttat gccggtaaaa ccgaacagca
    12241 ctacgccgtc gatattacgc cgtttcagca cccccaaatg ttcggcaact aattgtgggg
    12301 aaaactgatt ttccatcatg attggatcgt aaccttgttc atagaacgct ggcagcatgg
    12361 tttgaacggc gagattttct gacaacgaat ccagacgggt aacaatgatg gcgaccactt
    12421 tatcgctttg cccacgcata gcgcgcgcag agcgggaagg ggaaaatcca tgctgattca
    12481 tcactgcttc aacacgctcg cgcgtgcgct gactcacgcc gctttcgtta ttcagcaccc
    12541 gggaaaccgt agatttcccc acgccgctta agcgcgcgat gtctttgatg gtcagccgat
    12601 tttgcatcct gttgtcccat aacgtgttgt ttaattattt gagcctaact ttacccgtgc
    12661 attcagcaat gggtaaagtc tggtttatcg ttggtttagt tgccagcagg tattatatcg
    12721 ccataaatgc tacgaatatt attggattct ccttattatt tgcggcgctt ttttcactta
    12781 ccggaggtta tatggaacct gatcccacgc ctctccctcg acggagatta aaacttttcc
    12841 ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt ttaatgtccc
    12901 tgctcagctt tattaccttc aggtaaggct tcgccacgcc tgaagacatt tctgtactgt
    12961 ttcagacagt gcggagggac tccttatgtt taaagaaatt tttacccggt tcattcgcca
    13021 tttaccttcc cgtctggttc atcgcgatcc attgcctggc gcgcagcaga cagtgaatac
    13081 ggcggtcccg ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc ccgaagaaga
    13141 attgtggaaa acgttcgaca cccatccgga aggattaaat caggcggaag tggaatctgc
    13201 ccgcgaacaa catggtgata ataaattacc cgcacaacaa ccgtcgccgt ggtgggtaca
    13261 tttatgggtc tgctatcgca acccctttaa tattttgctc accattcttg gtgctatttc
    13321 ttacgccacg gaagatttat ttgccgcagg tgttatcgcg ctaatggtcg ctatttctac
    13381 gttgctgaac tttattcagg aagctcgttc caccaaagcg gcagatgccc tgaaagcgat
    13441 ggtcagtaat accgcgacgg tactgcgagt aattaacgac aaaggcgaaa atggctggct
    13501 ggagatcccg atagaccagc tggtgcccgg cgatattata aaactggcgg cgggggatat
    13561 gatcccggca gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc aggcgtcgtt
    13621 aaccggtgag tctctgcccg tggaaaaagc cgctactact cgccagccgg agcacagcaa
    13681 tccgctggag tgcgacaccc tgtgttttat gggcaccacc gtggtgagcg gcacggcaca
    13741 agcaatggtg attgctacag gagtcaacac ctggtttggt caactggcgg ggcgtgttag
    13801 tgagcaggaa agcgagccga atgcctttca gcaagggatc agccgcgtta gtatgctgct
    13861 gattcgcttt atgctggtga tggctccggt ggtgttgctt atcaacggtt acaccaaagg
    13921 cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg gtaggcttaa cgccggaaat
    13981 gttgccgatg attgttacct cgacgctggc gcgcggcgca gtaaagctgt cgaaacagaa
    14041 agtgatcgtc aaacatctgg atgccattca gaactttggc gcaatggata ttctgtgcac
    14101 tgataaaacc ggcaccctga cgcaggacaa aattgtgctg gagaatcata ccgatatctc
    14161 cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg aacagtcatt accagaccgg
    14221 acttaaaaat ctgcttgata ccgcggtgct cgaaggtacg gatgaagagt cagcgcgttc
    14281 gctggccagc cgttggcaaa aaattgatga gattccgttt gatttcgagc gtcgtcggat
    14341 gtcggtggta gtggcagaaa ataccgaaca ccatcagctg gtttgcaaag gtgcattgca
    14401 ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtac cgctcgatga
    14461 caccatgctg cgtaagatta agcgggttac tgatacgctg aatcgtcagg ggctgcgcgt
    14521 ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga gattaccagc gggcggatga
    14581 atccgacctg atcctcgaag gatatattgc tttccttgat ccgccaaaag agacaaccgc
    14641 tccggcactg aaggcattaa aagcgagtgg aattaccgta aaaatcctta ctggcgacag
    14701 tgagttagtt gcagcgaaag tgtgccatga agtggggctg gatgcgggag aggtggtcat
    14761 tggtagtgat attgaaacgc tatctgacga tgaactggca aatctcgcac agcgcaccac
    14821 gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt gtgaccttac tgaagcgcga
    14881 agggcatgtg gttggcttta tgggcgatgg tattaatgat gcgcccgcct tacgcgctgc
    14941 ggatatcggc atttctgtgg acggcgcggt agatattgcc cgtgaagcgg ctgatatcat
    15001 cctgctggaa aaaaacctga tggtgctgga agagggggtt attgagggac gtcgcacttt
    15061 cgccaacatg ctgaaataca tcaaaatgac ggcgagttct aacttcggta atgtgttcag
    15121 cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg ttgccgttac acttgcttat
    15181 tcagaacctg ctgtacgatg tgtcacaggt ggcgatcccg tttgataacg tcgacgacga
    15241 gcaaattcaa aagccgcagc gttggaatcc ggcggatctg gggcgcttta tggtcttctt
    15301 cggaccgatc agctcgattt tcgatattct gacgttttgc ttgatgtggt gggtattcca
    15361 tgccaacacg ccggaaacgc aaacgctgtt ccagtcgggc tggtttgtgg tgggattact
    15421 gtcgcaaacg ctgattgtgc atatgatccg tacccgccgt gtgccgttta ttcagagctg
    15481 tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg attgtcggga tcgcattgcc
    15541 gttttcaccg ctggccagtt atctgcagct gcaggcgctg ccgttaagct atttcccgtg
    15601 gctggttgcg attctggcag ggtatatgac gttaacccag ttggtgaaag ggttctatag
    15661 ccgtcgttac ggctggcagt aaagaataaa ctgggcatgg tggcccggat tcagattgca
    15721 gacaaagtga gctttgttca tgccggatgc ggcgtaaacg cccaatccgg cctacaaatt
    15781 caataaattg caggaatcac gtaggcctga taagcgtagc gcatcgggcg attttgcttt
    15841 tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta gcgacgaaca
    15901 gcgattgctt cgatctcaat cttcacgtct ttcggcagac gggcaacttc aacgcaagaa
    15961 cgtgccggga aagtggcgtt gtgttcggtg aagaaggctt cgtaagtggc gtttacggtt
    16021 gcgaagtcgt tcagatcttt cacgaacaca gtggttttaa cgatgtcgcc cactttcagg
    16081 ccagcggctt cgacgattgc ttttacgtta tccagcgact gacgtgcctg tgcagcgacg
    16141 tctgccggta cttcaccagt tttcggattt accgggatct gaccggaggt gatgatcata
    16201 ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt ttccgtcgcg
    16261 atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct cattataggg
    16321 agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg ggaaaactct
    16381 ttttcacagt atttgcattt aagcgcgatg tcattggcgc gttttcgcac ggcaaagctg
    16441 gatgaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag cacattgtcg
    16501 atgcgctccg gcagacttgg gcgcgattta cccaccactt catagttgtc gatacggtta
    16561 accgtggctt gcggtgcata caatgccagt tggtctactt gatcttcact caaaaaggta
    16621 ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaaatt cagaccaatg
    16681 gtgatgcgct gatctgtttc ggtcaacttg aacagactca acagcttaaa accgatctgg
    16741 gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa tttattatcg
    16801 tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag tgccagtaac
    16861 gcctggcgag cgaaaattcc gttgcctgct tgctggaagt accaggcgtg aggcgtttta
    16921 tccacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt catattggct
    16981 ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt ggcgtactcg
    17041 gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac ttccgccatc
    17101 acttcttcaa tagagctgtg cagactccag gcgatccctt tttcatcgag catatccaga
    17161 atgtattgcg gcattgccag cgcatccggc gcgataaagt aaaaacggtt gccgtcgaac
    17221 ttcgctagcg cctgggtcag ggagtgaacg gtgcggccat atttcaggtc accaaccatt
    17281 gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tggtgaataa gtccagcaag
    17341 gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac attgccagaa
    17401 aattcggtgg ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat cgcatcgacg
    17461 taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cttttttacc cagtgatgta
    17521 ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcatgga agtttcgaaa
    17581 gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt gtgcttcaac
    17641 agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag attaaggtca
    17701 tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt agccatcttt
    17761 tatctcctga cgcctaggca aaaaaaagcc cctcgattga ggggctggga atgggtgatc
    17821 aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac aaaatgtcga
    17881 acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat agccagaagc
    17941 ggtgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa ttaaaagtca
    18001 aaatatatgc atttttattt tctatgattc tggaggggaa gtgtcgttcg gtatcaataa
    18061 ttgcagacat ttcctgcttt tttctttttc attttcacgc aatcagaaat gcattttatt
    18121 ccattcatcc gacttatagg gcggagggtg aaaacgaacg gctaacacta ttgcttatag
    18181 ttcagggata cgcgctatca ctttaatttc aaaatcaaag cctgccagcc aggtaacacc
    18241 caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca ccgtcatgat
    18301 gtcttcaaat tgtttttccg gatcggtatg aaagctcgta acatcaatga tatcgtcaaa
    18361 agtgcatccc gcagctgcca gggtcgcatg caaattatca aaggccagtc tgacttgttg
    18421 ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg aaacaaacag
    18481 caaatcgccg gaacgaatag ccgcagaata acgatgctca gcatacagtg aatgtcggcc
    18541 agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta cgacatgaac
    18601 agaagattta tatttaccgg gagccgcttt tagggggacg acgttagtaa acaaaaccca
    18661 gacatcatgg ataatggctg ggcttaattg agcgtagtcg gttatgcgcc aaacgcgcca
    18721 tcaatggtat gcatcgcgcc ggtaacaaaa ctggcttctg gccctgctaa ccatgcgacc
    18781 ataccagcga cctcttccgg ttgcccatgt cttttgatgg ccataaaact atgcaacata
    18841 tcgcgcattg gcccgttggc gggattaacg tcggtatcaa ttggtcctgg ctggacgacg
    18901 ttgatggtga tccctcgcgg tccaaaatca cgggccagcc cgcgcgccat gccttgcagg
    18961 gcagatttac tggcggcata agcagccatg cctgcaacag gcatacgatc gccattcacg
    19021 gagccgatga ttaagatgcg cccgccttcg ggcatctgcc gggcggcttc aacagaggca
    19081 tgataaggag catgaatatt gattttgaaa aggcgatcaa tatcgtcggc atttaattcc
    19141 agggcatcgc caaagacgcc aatacctgca tttaccacca ggatatccaa tgcgccgctc
    19201 ttacgaacga catcaataac agcgtctctg tcagcactat ctgtgaatac tgctgtcgct
    19261 ccagtctctt gtgccagacg ttcagcggca tctttcgacc ccgcataggt gaatcgtaca
    19321 ttggccccat cggtgacgaa acgacgtacg atagcggcac cgattccacg actgccaccg
    19381 aggatgagaa ctgtcttacc ggtaaaagcg cccataagga ctccttgatt tattatgtaa
    19441 catgcattac aaaactgttt taactttctg tcaacaagtt ttgtaatggt cactaaaaaa
    19501 caatctcgtg ttccaggtcg tcccagacgt ttcgctcctg agcaggcagt ctctgcggca
    19561 aaagtgcttt ttcaccaaaa aggtttcgat gcagtaagtg tctctgaagt tactgattat
    19621 cttggtatta accccccgag cctctacgcg gcttttggca gtaaggctgg gttatttagc
    19681 cgtgtactca atgaatatgt cggtacggaa gctattccgc ttgccgatat tcttcgtgat
    19741 gatcgtccgg taggcgagtg cctggttgag gtattaaaag aagcggcgcg cagatatagc
    19801 caaaacggcg gctgcgctgg ctgtatggtt cttgaaggta ttcatagtca tgatccacaa
    19861 gcgcgtgata ttgccattca atattatcac gccgcagaaa cgaccattta tgactatatc
    19921 gccaggcggc atccacaaag cgcacaatgt gtgactgatt ttatgagtac cgtgatgtca
    19981 gggctttctg cgaaggcacg ggaggggcac tccatcgaac aactctgtgc aacagctgcc
    20041 ctggcggggg aagcgataaa aactcttctc aaggagtgat gctggccttg atccgaaagg
    20101 cgggaacgcg cctgccgata agttgtgata agacaataat tcacgcgtta aggctggcgg
    20161 aattgatcat cttttcgtat aacgatagaa atgaaacatt gttttaatta aggagtggaa
    20221 aagatgatca tcggaaatat tcataatctt cagccgtggc taccccagga gttacgccag
    20281 gcgattgagc atatcaaagc acacgttacg gcagaaacgc caaagggcaa gcacgatatc
    20341 gaaggcaatc gtctgtttta tcttatctcg gaagatatga ccgagccgta cgaagctcgc
    20401 cgtgcggagt accatgcccg ctatcttgac attcagattg tgttaaaagg tcaggaaggc
    20461 atgaccttca gcacgcaacc tgcaggcgcg ccggataccg actggttagc tgataaagac
    20521 atcgcatttt tgccggaagg cgttgatgag aaaacagtta tcctaaatga aggtgatttt
    20581 gttgtgtttt atccggggga agtgcataaa ccgctgtgcg cagtgggcgc gccagcccag
    20641 gttcgcaaag cagtagtgaa gatgctgatg gcgtgatgac ttttcgccgt aaataactcc
    20701 aggtttacgg cgagtttgtg aaaagagcgt tttttgatat ttttttgtga gtaaaatttg
    20761 taatgcttag acgttcttat taactcaagg agttcgtcat gagcaaaata tcagatttga
    20821 attattctca acacattaca ttagccgaca attttaaaca aaaaaatgaa gccttagata
    20881 cctggtatgt gggaatgaat gattttgccc gaattgccgg ggggcagaat agcagaagca
    20941 atattctttc tcctagagca tttttggagt ttttggcaaa gatatttacc ctgggttatg
    21001 tggattttag caaacgctcc aacgaagcgg gtagaaatat gatggctcat attgagttct
    21061 catcttattc taaagatact gatggcaatg aaaaaatgaa gttttacatg aataatcctg
    21121 aaggggagcg agcggattta tctaaggtga agacagaaat tacacttgcc tcagcttcta
    21181 ctaaagggat tcgtgaagga catacagtca ttatatttaa acaatctgat ggttcgacta
    21241 atcgtatcgt tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat
    21301 tactaacaag gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt
    21361 aaataatcat cagaagttaa ataatctaca gaagttaaat aatatac
//
LOCUS       DADKXM010000058        21329 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.058, whole genome shotgun sequence.
ACCESSION   DADKXM010000058 DADKXM010000000
VERSION     DADKXM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.058"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            11..865
                     /locus_tag="JQR05_002185"
     CDS             11..865
                     /locus_tag="JQR05_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY5324356.1"
                     /translation="MFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLES
                     IFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQ
                     VGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSE
                     SESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDK
                     TFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSGGGGSSG
                     GGGASGRW"
     gene            complement(925..2223)
                     /gene="eno"
                     /locus_tag="JQR05_002186"
     CDS             complement(925..2223)
                     /gene="eno"
                     /locus_tag="JQR05_002186"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY5324357.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2311..3948)
                     /gene="pyrG"
                     /locus_tag="JQR05_002187"
     CDS             complement(2311..3948)
                     /gene="pyrG"
                     /locus_tag="JQR05_002187"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY5324358.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4176..4967)
                     /gene="mazG"
                     /locus_tag="JQR05_002188"
                     /pseudo
     CDS             complement(4176..4967)
                     /gene="mazG"
                     /locus_tag="JQR05_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(5063..7297)
                     /locus_tag="JQR05_002189"
     CDS             complement(5063..7297)
                     /locus_tag="JQR05_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY5324359.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7345..8646)
                     /gene="rlmD"
                     /locus_tag="JQR05_002190"
     CDS             complement(7345..8646)
                     /gene="rlmD"
                     /locus_tag="JQR05_002190"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY5324360.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLGALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLQEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8703..11459
                     /gene="barA"
                     /locus_tag="JQR05_002191"
     CDS             8703..11459
                     /gene="barA"
                     /locus_tag="JQR05_002191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY5324361.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPPSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11691..13031)
                     /gene="gudD"
                     /locus_tag="JQR05_002192"
     CDS             complement(11691..13031)
                     /gene="gudD"
                     /locus_tag="JQR05_002192"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY5324362.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13052..14392)
                     /locus_tag="JQR05_002193"
     CDS             complement(13052..14392)
                     /locus_tag="JQR05_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY5324363.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14394..15746)
                     /locus_tag="JQR05_002194"
     CDS             complement(14394..15746)
                     /locus_tag="JQR05_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324364.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKSVAGQ
                     "
     gene            complement(16180..16629)
                     /locus_tag="JQR05_002195"
     CDS             complement(16180..16629)
                     /locus_tag="JQR05_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY5324365.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16647..17429)
                     /gene="truC"
                     /locus_tag="JQR05_002196"
     CDS             complement(16647..17429)
                     /gene="truC"
                     /locus_tag="JQR05_002196"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY5324366.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17429..17758)
                     /locus_tag="JQR05_002197"
     CDS             complement(17429..17758)
                     /locus_tag="JQR05_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY5324367.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18380..18925)
                     /gene="syd"
                     /locus_tag="JQR05_002198"
     CDS             complement(18380..18925)
                     /gene="syd"
                     /locus_tag="JQR05_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY5324368.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18993..19841
                     /gene="queF"
                     /locus_tag="JQR05_002199"
     CDS             18993..19841
                     /gene="queF"
                     /locus_tag="JQR05_002199"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY5324369.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19953..21317
                     /gene="ppnN"
                     /locus_tag="JQR05_002200"
     CDS             19953..21317
                     /gene="ppnN"
                     /locus_tag="JQR05_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY5324370.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5233 a         5687 c         5099 g         5310 t
ORIGIN      
        1 tttcatacta atgttcactt tcgtatgttc ctttgttgca gcccaaccaa ctattgtccc
       61 acaattacag caacaagtta cgggtcttac gagtagctta aactcacaag aaaagaaaga
      121 actgacgcac aagttagaat ctattttcaa taatacccaa gtgcaaattg ctgtattaat
      181 tgtttccaca accaaagatg agacaattga acaatatgct acaagagttt ttgacaattg
      241 gcgtttagga gatgccaaac gtaatgatgg gatactgatc gttgttgcct ggtcggatcg
      301 cactgtccgc atccaggtag gttatgggct ggaagaaaag gtaacagatg ctctggctgg
      361 agatatcatc cgtagcaaca tgatacccgc atttaaacaa caaaaattag ctaagggatt
      421 agagttagct ataatcgctt tgaataacca actcacttca caacaccaat atccggctaa
      481 tccttcagaa agtgaatcaa cgtcttccac tgatcattat tactttgcta ttttttgggt
      541 atttgcagtg atgttcttcc ctttctggtt ttttcatcaa ggcagtaatt tttgtcgcgc
      601 atgtaaaagt ggagtctgta tttcagcaat ccatctttta gatttattcc tgttctccga
      661 taaaactttt tccattgctg tattttcctt cttttttact ttcaccatat ttatggtctt
      721 tacctgttta tgtgtacttc agaaaagagc atctggtaga agttatcatt cagacaacag
      781 cggttccgct ggaggctcaa gtggcggcgg atttagcggc gggggcggtt cttctggcgg
      841 tggcggcgca tccggccgct ggtaactcca ccataaaaaa tgccagcccg aaggctggca
      901 ttatcaaatc agataaagtc agacttatgc ctggcctttg atctctttac gaccgttgta
      961 cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt tagcaacacg
     1021 gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa cagccagatc
     1081 agcgatggta gcatcttcag tttcaccaga acggtgagag ataactgcag tgtagccagc
     1141 atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct ggttgaattt
     1201 gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct tggtgttggt
     1261 tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg tctggtatgc
     1321 gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gaaacgatcg ggtactgttt
     1381 ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aacgctttgt tgccttcgcc
     1441 agccagaacg tatttaccat ctttgtagaa ttcagaagct gcgcagtcca tcgccagagt
     1501 gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa cagccagagc
     1561 ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaacag cagtgttcat
     1621 gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca tgcggatggc
     1681 ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat caacgttgtt
     1741 gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg agtatttgcc
     1801 cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atacctttag cagctgcagc
     1861 agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt tggatttgtt
     1921 ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt tagcatcttt
     1981 gccaatcagc gcctgagcga tcgggccgtt taccgcagca acagctttgg ttacgccttt
     2041 acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg aaccagtaga
     2101 agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta cttcggcttc
     2161 aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta cgattttgga
     2221 cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa cgcgtaccca
     2281 gggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct ggaactcgct
     2341 ggcggctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag aagtaaactc
     2401 cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga tctcgaccaa
     2461 ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct gtttcaacag
     2521 catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg cattgtatag
     2581 ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac gcatggtacc
     2641 gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc gccactcggt
     2701 aatcagcgcc acaaccgggt acttacagtc tggcacaaat tctgtagagt tggcgttctc
     2761 catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca ggcaaatgcc
     2821 tagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc cttctacgcc
     2881 acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga tttcaacgcc
     2941 gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac gattcttcag
     3001 cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt caatgtactt
     3061 gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga taacctgttc
     3121 ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa tataatcgtc
     3181 cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt ctttcagaga
     3241 aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt cgttcgccgg
     3301 tacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg agagcagctc
     3361 ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca tgtacggcac
     3421 cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca tctggcgaat
     3481 agcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt ctaccagtac
     3541 tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag tgatgtgcgg
     3601 aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca gaacgtcaga
     3661 gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa tgaaacgctc
     3721 gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca cttccccgtg
     3781 ttggattggg ctcatagtac ctggatcgac gttgatgtaa ggatccagtt tcatgatggt
     3841 cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa tgcctttacc
     3901 cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc tgaacctgag
     3961 aagttaggtt gaaaagacga tggaataacc aagacgggaa agcagtatac ccgaacatga
     4021 cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga aatcaggagt
     4081 gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt gtcatttaaa
     4141 aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc ctgccgtttt
     4201 acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc cagtccacgc
     4261 gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt cgctttttgc
     4321 aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc aaacagcagg
     4381 tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc ctcgtacatc
     4441 acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt ccagtcgaag
     4501 ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg taaactacga
     4561 ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt tttgatttgc
     4621 tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa aacatgcgga
     4681 tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc aaagcgccct
     4741 tcttcctgag ccatttgcgc gtaaaacacc acctagaata gcagatcgcc cagttcaccg
     4801 cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt ttcttcaagg
     4861 gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc gttttccgga
     4921 tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga attgtcctga
     4981 aaattgcggg tctgtcaggt gtaatacctt cgagtaaaag atatcgctgg atacggctga
     5041 cgccatatcc agctatgtaa gactaactcc cgtgcaaccg acgcgcgtcg ataacatccg
     5101 gcacctggtt gagtttaccc agcacgcgcc ccagcacttg caggttgtaa atctcaatgg
     5161 tcatgtcgat ggtcgccagt tgctgtttgg tgtcgctacg gctggcaacg ccaagcacgt
     5221 tcaccttctc gttggcgaga atggtcgtga tatcacgtaa caacccacta cgatcattag
     5281 ccaccacgcg gaccaccagc gaatatccag cggagtagct ctcaccccat accgcgtcaa
     5341 caatgcgttc tggcgcatgg gagcgcagtt ccgccagttg ttcgcaatcg gcgcggtgta
     5401 ctgaaatacc gcgtccctgg gtaatgaagc cgacaatctc atctccagga atcggctggc
     5461 agcagcgcgc gatgtggtgc atcaggttac caacaccttc aaccaccaca cgaccgttat
     5521 ctttactgcg gttttgcggt gtgtagcttt tttgctgaag ctgtttcagc gcggcggcgt
     5581 cctgctcttc ggcactcggc ttattaaatt gcgattgcag gaagttcacc atctgattga
     5641 gacggatatc cccgccacca atcgccgcca gcaactcgtc gacatcattg aagttgtaac
     5701 gcggcagcag atgtttttct gcttctttca ggctgatccc cagatgctcc agctcgtcgt
     5761 caaggatttg ccgcccagcc agaatgtttt tgtcacggtc ctgtttacgg aaccaggcgt
     5821 gaattttcga acgcccacgg ctggttgtga cgtaaccgag gtttgggttt aaccagtcac
     5881 ggctggggtt cggctgtttc tgggtgataa tttcaatctg gtcgcccatt tgcagctggt
     5941 aggtgaacgg cacaatgcgc ccgccaattt ttgccccgat gcagcggtgt ccgacatcac
     6001 tgtggatgtg gtaagcgaag tccagcggcg ttgatcccgc aggcaaatca acgacatcac
     6061 ctttcggcgt aaagacatac acccggtcgt caaacacctg actacgtact tcgtcgagca
     6121 tttcgccgga atcagccatc tcttcctgcc acgcaatcag tttacgcagc caggcaatcc
     6181 ggtcttcatg tcccgaacgt gcgccgccag ctgccgcgcc ctctttatat ttccagtgcg
     6241 cagcaacacc caactccgca tcttcatgca tctgtttggt gcggatttgg atctcaacgg
     6301 tttttccacc cggccccaga accacggtat gaatagactg ataaccgttt ggtttcgggt
     6361 tagcgacgta atcgtcaaac tcatccggca ggtggcgata gtgagtgtgc actatcccca
     6421 gtgcggcata acaatcctgt aaacgctcgg cgacaatacg taccgcacgc acatcaaaca
     6481 gctcatcgaa ggcgaggttc tttttctgca ttttgcgcca gatgctgtag atgtgtttcg
     6541 gacgaccata cacttcagct ttaacgcctt cagctttcat ctcagcgcgc agatggccga
     6601 caaactcttc gatatagtgt tcgcggtcga gacggcgttc atgcagcagt tttgcgatgc
     6661 gtttgtattc ggtcgggtgg agataacgga agcagtaatc ttccagctcc catttcagtt
     6721 gcccaatccc aagacggttc gccaacggcg cgtagatatt ggtgcactct tttgccgcca
     6781 gtacacgttc atcttccggc gcatctttta cttcgcgcag atgagcaata cgctccgcca
     6841 gtttgatgac tacgcagcga aaatcatcga ccatcgccaa taacatccgg cgaacgttat
     6901 cgacctgttc ggaggaaaca gaatcagtgt gcgtcgcttt cagctggcgg atcgccgcca
     6961 tatcacgcac gccgtgaata aggttaacaa ccgacttacc gacgctctca cgcagcacat
     7021 cttcgctgac tacgttggca tcagccagag ggaacagcag cgccgcccgc agcgtgtcaa
     7081 tgtccatact taatgtcgag aggatctcca ccatctcaac accacgccac aataacagac
     7141 tggcatccgg atgcccctgc gtctgttgca gacaatacgc ccaggtttcg gctaagcact
     7201 cacacgactt ctggctggta atacccagac ttgcgatcca tttttccgga tcaaattcac
     7261 cagccttatt gatatgtgca cttcttaccg caaccatcgt cctctccttt agggaccaga
     7321 cctgccgaaa tcggcaaatc gtaactattt aacgcgcgag aaaagtacca tcgattccag
     7381 atgtcccgtg tgtgggaaca tatccagcat cgccagtcgc gcaatggtat atcctgcttt
     7441 taataacgct tcgctatccc gagccagcgt tgcagggtta caggatacat aaactatacg
     7501 aataggttcc agttttataa tttgctgcat aacacctgcg gcacctgctc gcgccgggtc
     7561 cagcaacact ttatcgaagc cgtttttcgc ccacggctgc tttgtgacat cttcttgaag
     7621 attttcgtga taaaacgtca cattctgtaa gccattaaga cgcgcattct gctggccttt
     7681 ttccaccagc gccggaacac cttctacacc gaccacactg gcagcttgtg tcgccaatgg
     7741 cagtgtaaag ttgcccatac cgcagaacag atccagtacg cgatcttcag gttgcacatc
     7801 cagccattcc aacgcacgcg ctaccatttt ttggttcaca cccgcattga cctgaataaa
     7861 atcgcgcggg ctaaaagtta agcgcaaccc gtttgagtca taccagggca tctcaccaga
     7921 gacggtttcg agtatctcac tatcgggggc gagatacaga tccaggcctt cagaatgcga
     7981 aaagcgttcc agtttttcgc gatctgccga acttagcggt gcggtatggc gcaaaatcat
     8041 cagcgtgccg ctggttgcct gtaccagttc aacatgacca agatggcgca tagcttgtaa
     8101 gctgcccaga catgccctga ctttgggcag caatgctcca agttggggcg ctaaaatggg
     8161 gcattgtttg acgtcgacaa tgtcactgga gcccgctttg cgaaacccca tctgaagttg
     8221 ctgtgttttc ggtaagtagt tcagacttaa acgcgcgcgg cggcgatagc cccagggaac
     8281 atcggcgatc acttcagaga catcgtgttt cattaatcgg gcgagtgccg cacttttgct
     8341 tcgctgctgt aaatccacgc tggcgtgttg ttgctgacag ccgccgcata cgccaaaatg
     8401 aggacagcgt ggcgtttcgc gttccgggct atcgcttaac cggcgtacga ctttagcgcg
     8461 ggcatactgt tttttatctt cagtaacagc aacttccgcg ttttcctgcg gcaataatcc
     8521 ggggataaat aacgttttgc cgttatgtcg cgccacgccc tgaccaaaag agtcgaggtc
     8581 gttgactgaa acggttatga tctgacgcgt cgtcgtgcgt cgttttgcag agtagaattg
     8641 cgccattggc gagactttct caatttaaca gtgtgacctt aattgtccca taacggaact
     8701 ccatgaccaa ctacagcctg cgcgcacgca tgatgattct gatcctggca ccgaccgtcc
     8761 ttattggttt attgctgagt atctttttcg tcgtgcatcg ctataacgac ttgcagcgtc
     8821 aactggaaga tgccggtgct agcattattg agccgcttgc agtttctact gaatatggca
     8881 tgagcctgca aaatcgcgaa tctatcggtc agttaataag cgtactgcat cgtcgccatt
     8941 ccgatattgt tcgcgcgatt tcggtttatg atgaaaataa ccgactcttt gtcacctcca
     9001 attttcatct tgatccccca tcaatgcagc tcggcagcaa cgtgccgttt cctcgccagc
     9061 tcactgtcac tcgtgacggc gatattatga tcctccgcac gccgattatt tctgagagtt
     9121 actcccccga cgaatcgccc agtagcgatg ccaaaaatag tcaaaatatg ttgggatata
     9181 ttgcgcttga gctggatctt aaatcggttc gcttgcagca atataaagag atctttattt
     9241 cctgcgtgat gatgctgttt tgtatcggta ttgcgcttat ttttggctgg cgcttaatgc
     9301 gcgatgtaac cggtccgatt cgcaacatgg tgaataccgt cgaccgcatc cgtcgcgggc
     9361 aactcgacag ccgggtggaa ggatttatgc tcggcgagct ggatatgctg aaaaacggta
     9421 tcaactcgat ggcaatgtcg ctggctgctt atcacgaaga gatgcagcac aatatcgacc
     9481 aagcgacgtc cgatctgcgt gaaacgctgg agcagatgga aattcagaac gttgagttgg
     9541 atctggcgaa aaagcgcgcc caggaagcgg cgcgtattaa atccgagttt ctggcaaata
     9601 tgtcacacga gctgcgtaca ccactgaatg gtgttattgg ctttacccgc ctgacgctga
     9661 aaacagaatt aacaccaacg cagcgcgatc acctgaatac gattgaacgt tcggcaaata
     9721 atttgctggc aattattaat gatgttctcg acttctcgaa actggaagca ggtaagctga
     9781 ttctggaaag tattccattc ccactacgca gcacgctgga tgaagtcgtt actctgctgg
     9841 cacattcttc tcacgataaa gggttggaac tgacgctcaa tattaaaagc gacgtgcctg
     9901 ataacgtgat cggcgaccca ctgcgattac agcaaatcat cactaacctg gtggggaatg
     9961 caattaaatt caccgagaat ggcaacattg atattctggt agaaaaacgt gcgctgagta
    10021 ataccaaagt gcagattgaa gtgcagattc gggataccgg cattggtatt cctgaacgcg
    10081 atcaatcgcg cttattccag gccttccgac aggctgatgc cagtatttcc cgccgtcatg
    10141 gtggcaccgg gctggggctg gtgattacac aaaaactggt taatgaaatg ggcggcgata
    10201 tttcgttcca tagccagccg aatcgcggtt caactttctg gttccacatt aatctcgatc
    10261 tgaacccgaa cattattatc gaagggccat ccatccagtg cctcgcaggt aagcgcctgg
    10321 cctatgtcga accaaactcc gcagcagcgc aatgcacgct ggatatttta agtgaaacgc
    10381 cgctggaagt ggtttatagc ccaacgttct ccgcgctgcc tcccgcgcat tacgacatga
    10441 tgttgttagg catcgcggtg accttccgcg agccgctaac aatgcaacat gagcgattag
    10501 cgaaagcggt atcgatgacc gatttcctga tgctggcact tccttgccat gcacaagtca
    10561 atgctgaaaa actcaagcaa gatggtatcg gcgcgtgtct gctgaaacca ttaacaccta
    10621 cgcgcctgtt gcctgccctg acggaatttt gtcatcacaa acaaaacacg cttttgcctg
    10681 taaccgatga aagtaagctg gcaatgacag tcatggcggt tgatgacaac cccgctaacc
    10741 tgaaacttat cggcgcattg ttggaagata tggtgcaaca tgtggaactt tgcgatagcg
    10801 ggcatcaggc agttgaacgg gcgaaacaga tgccgttcga tttgatctta atggatattc
    10861 aaatgccgga catggatggc attcgggcct gcgaactcat ccaccaactc ccgcatcagc
    10921 gacaaacgcc ggttatcgcg gtaacggcgc atgcaatggc cgggcaaaaa gagaagctgc
    10981 ttggcgcagg gatgagcgat tatctggcga aaccgattga agaagagcga ttgcataatt
    11041 tgttgttgcg ctacaagcct ggcagcggta tttcgtctcg cgtcgtgacg cccgaagtca
    11101 acgaaattgt ggtgaacccg aatgcgaccc tcgactggca actggcacta cgccaggcag
    11161 caggaaaaac cgatttagcg cgcgatatgc tgcaaatgtt actcgatttc ctgcctgaag
    11221 ttcgcaacaa agttgaggaa cagctggttg gagaaaaccc ggaaggcctg gttgatttga
    11281 ttcataaact gcatggcagt tgcggctata gcggcgtacc acgtatgaag aatctctgcc
    11341 agctgatcga acaacagcta cgtagcggta ctaaagaaga agatttggag ccggagctgc
    11401 tggaactgtt ggacgagatg gataatgtcg cgcgcgaagc cagcaaaatt ctcgggtaac
    11461 ggacgtcttg tcgagtagca atcggctttt tatgacgctg gtttcagatt ggtgacaaag
    11521 tgcctgtgtt tatgccggat gcggcgttaa cgccttatcc ggcctacaaa accaattaaa
    11581 ttcaatgaat taaaaaatga tgtagacctg atacatagca caacaggcta ttttgcgttt
    11641 agcatcagtc tcaaaccggc tccagataga gccggttttg gttttctgtc ttaacgcacc
    11701 atgcacgggc gcttgttatc gaacgtccag ccaggaatca gatactgcat tcccatcgca
    11761 tcgtcacgcg cgccaagccc gtgtttctga tacagctcat gggctttcat cacttgatcc
    11821 atatcgattt ctacacccag ccccggtttt tctggcacct gtaccagccc gcctttgatc
    11881 tcaaacggtt ctttggtcag gcgctgatta ccttcctgcc agatccagtg cgtatcaata
    11941 gcagtaattt tacccggtgc agcggcggca acatgggtaa acatcgccag ggaaatatcg
    12001 aagtggttgt ttgagtgtga accccaggtc aggccaaatt catggcacat ttgcgccaca
    12061 cgtaccgaac cttgcatagt ccagaaatgc ggatccgcca gcgggatatc aacggattgt
    12121 aacgagagcg tatggcccat ctgccgccag tcggtggcga tcatattggt tgcagtcggc
    12181 aggccagtcg cgcgacggaa ctctgccatc acttcacgcc cggagaaacc ttgctccgca
    12241 ccacacggat cttctgcata agccagcgaa cctttcaggt atttaccaat tttaattgct
    12301 tcgttcagcg accaggcacc gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc
    12361 gccagtgcca caatagactc ggcttcttct tccccggcca gtacaccgcc tttcagtttg
    12421 aaatcgttga agccatattt ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc
    12481 gtcatcgctt cttcatgacg caggcgatac cagtcgcatg agtcatccgg ctggctttga
    12541 tacggcagag gtgtggcttt gcgattaccg acgaagaaca gataaccgag catttcgact
    12601 tcgctacgct gttgaccatc gcccagcagc gatgccacgt ttacccccag atgctgcccc
    12661 agcagatcca gcattgccgc ttctatcccg gtaactacat gaatagtggt acgcaaatca
    12721 aatgtctgca aaccgcgccc actagcatca cgatcggcaa aagtattacg caccagcgtc
    12781 agaacgtttt tgtattcacc cagcgtttta cctaccacca gcggaatcgc atcttccagc
    12841 gttttacgga ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc ggaattatct
    12901 ttgataatca caatattacg cgtgaagaac ggtgcgtgtg caccactcag attcatcagc
    12961 atactgtcat gacccgccac cgggatgacc tgcatttcag taacaacagg cgtcgtaaat
    13021 tgagaactca tatttatgtc ctttttcaga atcaatgacg gccgaaaacg ggacgtttac
    13081 ggtcaaaggt ccagccgggg atcaggtact gcatcggacc tgcgtcgtta cgcgcaccgc
    13141 aaggcagacg tttataggcc tcatgtgcct tttgtacctg ttcccagtcc agttccacgc
    13201 ccagaccggg cgcatcagga acggcaattt ttccgttttt aatctccagc ggatttttgg
    13261 tcaggcgaca atcgccctcc tgccaaatcc agtgggtatc gatagcggta ggattacccg
    13321 gtgccgccgc gcccacatgg gtaaacatcg ccagagagat atcgaaatgg ttgttggaat
    13381 ggcagcccca ggtcagcccc cagtcgtcgc aaagctgcgc cacgcggact gcaccggaaa
    13441 gcgtccagaa gtgcggatcg gcaagtggaa tatctaccgc attgagcatc accgcatgac
    13501 ccatttcgcg ccagttggtg gcgatcatgt tagtcgcgac gggcaagccg gtcgcccgtc
    13561 gaaactccgc catcacttca cgtccggaga agccctgttc tgcgccgcat ggatcttcgg
    13621 cataggtaag aacatcattc agccctttgc ataaagaaat ggcttcatca agcagccatg
    13681 caccgttggg atcaacggta atccgcgcat ccgggaagct tttcttcaat gcacgaacag
    13741 tgtcgatttc ttgctcgcca ggtaacaccc cgcccttaag tttgaaatct ttaaagccat
    13801 agcgatcctg tgaggcttcc gccagacgca caacggcttc gctgttcatc gctttctgat
    13861 ggcgcaactg ataccactca tggttgcccg gcgtattttc cagataagga agatcggttt
    13921 tggtccgatc accgatataa aacagataac cgaggacggt aatagtctcg cgttgcttgc
    13981 ctggccccaa cagttcgcag accggaacat tcagcgcctt acctagcagg tcaagcaagg
    14041 cggcttccag cgccgccacg gcattaacgc gcaattcaaa agtccaggca cctttgccga
    14101 aggtatcaaa atcggctgcc tgattacctt tatgcacctg ctgaaccact ttattcaggc
    14161 gcgcaacttc ctggcccaga accatcggaa tagcatcgac cagtgtttga taaatcacct
    14221 ctccgcccgg cgcttcacca atgccggtat gcccggcgtt atcggtgagt accacgatat
    14281 tgcgggtgaa gtatgcgtta tgcgcaccac caatattgag caacatgctg tcatgtccgg
    14341 cgaccggaat gactttcata tcagtaataa caggactgga ttgtgtcgcc atcttattgc
    14401 cccgcgacag atttcagctc gatacgcttg atatcgccca ccagcaccag gtagctcagt
    14461 accgcgatta gggcatgaac accaacataa atcagcgccc cattaaacga gccagtcgtg
    14521 ccaacgatat aaccaattgc gattggcgtg acgatgccag aaatgttacc gaacatgttg
    14581 aacaggccac cggaaagacc gctgatctct tttggcgcgg tatctgccat tactgcccaa
    14641 cccagcgccc cgatgccctt accgaagaag gccagcgcca taaagccgat gatcatccac
    14701 tcaacgttga cgtagttgca gaacaccatc accatcgaca gcaacatgcc cattacgatc
    14761 ggtgttttac gcgcaatgtt cagcgatccc gtgcggcgca tcagccagtc ggaaataatt
    14821 ccacccagca caccgccgat aaaaccgcaa accgccggaa cggaagccac aaagcccgct
    14881 ttcagaatcg acatcccacg cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag
    14941 taagtcaggg cgttgataca gtactgaccg atataaacgc cgatcatcat ccgtgacccg
    15001 agcagctgtt tgatctgccc ccacttcacg ctgaacggca ctttaacttt ggtgttttgc
    15061 tgatccatat tgatcagcgc gccacccgcg gcgatatact ccagctcttt ctgatttacc
    15121 cccggatgtt gatttggctc gtggatgact ttcaaccaga taaagctgat gacaatgccc
    15181 agaccgccca taaagaagaa gacgtgtgac cagcccactt catgcgtcag ccagcccata
    15241 atcggcgcga agatcaccgt tgcgaagtat tgagcggagt taaaaatcga caccgccgtt
    15301 cccctttcct gcgccggaaa ccaggccgca acaatgcgac tgttgccggg gaaagatggc
    15361 gcttcagcaa gcccgaccag gaagcgcaga gtaaacaggg caacgataat gccgaatcca
    15421 ctaaagatat cgacgaagcc ttgcagcaag gtaaacatcg accagataaa gatcgaccag
    15481 aagtagacgc gttttgaacc aaaacgatcc agcaaccaac caccagggat ctgcccgata
    15541 acataagccc atgagaaggc agagaacaca tagcccattc ccacgggatc aaggccgata
    15601 tctttggcca tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat
    15661 gtgacgataa acaacatcac cactatccag taacgagcat tcgtgcgttt ttccacactg
    15721 ctcgcagcct gacttaaaga actcattgtt gcactcctga aaattcgcgt tagccacgct
    15781 cactctggac tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt tttgccgttt
    15841 ttataggtcg ttcgccgaat acggcgcgtg tttatatctg gcaatagcag tataaaaagc
    15901 gcgccatagc ggctcaccgt gcaacaacac aacattaatg cgttcaatga ggcccgattt
    15961 tggcattagc cctggacggt ggaatccact tcacggaaat gaaaacaaga acaagaaagg
    16021 aagggtaaaa acgaagaaat aaagaagagt atgaaatgga tcgcttgact ctaggcaaac
    16081 gccagtaaaa atccgcgcta tgaagcagtt tttactggca tttgcctgaa aagattcgat
    16141 tcagcaccgc taaaacgaca tttaccgctc gctgacatat caggacaaca gcgtgcccca
    16201 ctgttcgacc cacggatttg attccgtttc cggttccggg ttctcactgg cgtcaatcaa
    16261 cagcatttcg ccaacccgct gagcgctctg ttcctgcaac aaggcatcga actgtttgcc
    16321 gccattgcaa aaatttacat aactgctgtc gccgagcgca atcacgccat aacgcagatt
    16381 cggctggaag cccagactat ctttgattcc ctgaaagagc ggcacaatgc tatcaggaag
    16441 gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac ttatcctgat agggcagcca
    16501 gtcgcttaat tcaggatctt caaataccgt tgctttgtgg ccctgtgcgg tcagaattgc
    16561 ttctgcttct tccgccacta acagcgaatt tccgtacatg gtgccgacaa aaataccaat
    16621 ttccgccata ccgttttctc cctggattag gaacttatct ctccatcctg acccgatggc
    16681 gcactgaact caaccctttc attttcagga agcagaccgc gccagccaaa ttgtgataac
    16741 gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca gcggctcgcc agtaaaagga
    16801 tgcgtcaatg acaactgact ggcatgtagc attaaccgct ggaggccaaa atgctcagca
    16861 ccgctgcgat tctggcgtaa atcgccatgt ttgctatcgc caataatcgg atgacgcaaa
    16921 tgggcaagat gtcggcgaag ctgatgtttg cgtccggttt tcggctccag ttccaccagg
    16981 ccgtagcgcg tggtcgggta acgtccggtc gctaccggca tttctacggt cgccagaccg
    17041 cgataatgcg tcactgctgg ctgcgggcct ttatcttcgc gggcaaattt atcagcgatt
    17101 ttgtccagtt cttccaccag tggataatcc aacaccgctt cttccatcaa ccagccgcgc
    17161 acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa actgttgtgc cagcagccgt
    17221 ccggcctcgc tggataatcc catcaacaac acaccagaag tgggtcggtc cagacgatga
    17281 gcagtaaaaa catgctgtcc tatctggtca cgcacggttt gcatgaccac tactttctcg
    17341 tcgcgatcca gccagctacg gtgaaccagc cagccggagg gtttatttac cgcaaccagc
    17401 cattcatcct gatagagtat ttccagcatt agctcgcatc atccgcaaaa agagcatcca
    17461 gtttttccaa ctcagccaga ataagcgcgc gttgcggatg gtccgtcgcc agcgccattt
    17521 cataataggg tgcaacagca aaagcgcccg gtaacggctg gttgttattt aacaaatcgt
    17581 gcattcgcgg gatcagcccc cactgcaacc actccagtgg ttccatggtg tccataaaga
    17641 acggttgggt actattaaat tgatgcggct ggggttcatc gtttcgccag tgctgatgtt
    17701 cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac gcggtcatga gtggtcataa
    17761 agcaacctca ataagaaaaa ctgccgcgaa ggatagcagg aataaaaaaa gggagcactg
    17821 tattcacagt gctcccggtt cgtttcgcag cattccagct acttttgttg ctccctgctc
    17881 atccttgaca acttttcctc tggccttgcg gccaatcgtt catcctgaac tattgcttcc
    17941 tgctcacacc accccgatgt gatacttcat cctgaagtgt ccctggcctt cctgacccac
    18001 cgaatcatcc tgaccggttc tcattctcca tcctggaggt gtcctttaac gcgtcctgcg
    18061 tcatcctctt cgcttcatcc agaagccttt ccctgaaaca ccatcctggt gtgtcctgca
    18121 gaagtgtcat cattctgatg ttcacttcgt tgtctgactc cctgtcgacg aagatagaat
    18181 cgtctttttc gcgaagtctt acaaggcgct tacagacaat gccttaagga aaatttcata
    18241 tgaaagttaa gaattaacaa tcaacatttt gattttaaat gaaaataacg attcttatct
    18301 ttttaagtat tcgcttattt cctggcgatc tcctacagag tctgtaagag atctctcaca
    18361 accagatggg gtatttggat tacagaagag gcttaagttg attaaggaat tccgcaagat
    18421 ttgaggctaa atgcgtccgt ttacgtgttc ccagcgtctc tttgcacact tcaccggaga
    18481 gattacacac cgagatgacc tctaactctt cttccagcgt cgcgataaat agcgtgggcg
    18541 gcagttttag ccttttctga gtcaccagat gaccaatcag attttcctgc acccgacgga
    18601 agtcgtcttc actccaggtt tgcagcaatg tcagtttgat atcgccaaac tgcgcgtgca
    18661 tatccccggc aaactgagtc gtataaaagg tatgaattgt gggttgtatc acaatatcaa
    18721 aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa cggctggggt tgccagtata
    18781 cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac accgtacagt tcctcactta
    18841 acggccagct tttatgctct tcatgccacg catcgcagta gcgtgcagta aaatctttca
    18901 gggcttgtgc ggtcaaatcg tccactaatt tctctcttca cttaaaccag atacacttgt
    18961 cgtttagttt atctggttta tgacggtgaa acatgtcttc ttatgcaaac catcaggcac
    19021 ttgcgggcct gactcttgga aaatcaaccg attaccggga tacctatgac gccagcctac
    19081 tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg tctgaaagcg gataacctgc
    19141 cttttcacgg tacggatatc tggacgctgt atgaactttc ctggctgaat gcgaaaggtt
    19201 tgccgcaggt cgctgtcggt catgttgaac ttgattacac cagcgtaaat ctgattgagt
    19261 cgaagagttt taagctctat ctcaacagtt ttaaccagac gcgttttaat aactgggatg
    19321 aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc tcagggtaag attagcgtgg
    19381 cgttatatcg tcttgatgaa ctggaaggcc agccgatagg tcatttcaat ggcacttgca
    19441 ttgatgacca ggatatcact atcgataact atgaattcac tactgactat ctggagaatg
    19501 ccaccagcgg tgaaaaagta gtggaagaga cgctcgtcag ccacctgctg aaatcaaact
    19561 gcctgatcac ccatcaacca gattggggtt cgatccaaat tcagtatcgt ggacgccaaa
    19621 ttgacagaga aaaactgctg cgttacctgg tctcattccg tcatcacaac gagttccacg
    19681 aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt ctgccagcca gagaaattga
    19741 gcgtttacgc acgttatacc cgtcgtggcg gtctggacat taacccgtgg cgcagtaata
    19801 gcgattttgt cccatcgacc acaagactgg ttcggcaata aattttttct caattttgcg
    19861 tgctggattc acgcagaagg ttgtgaaagg tcatcaggca gggctattgt aatcaaaggg
    19921 aatgacgata ttcgtcccat aaggagtttt tcttgattac acatattagc ccgcttggct
    19981 ccatggatat gttgtcgcag ctggaagtgg atatgcttaa acgcaccgcc agcagcgacc
    20041 tctatcaact gtttcgcaac tgttcacttg ccgtactgaa ctccggtagt ttgaccgata
    20101 acagcaaaga attgctgtct cgttttgaaa atttcgatat taacgtcctg cgccgtgaac
    20161 gcggcgtaaa gctggaactg attaatcccc cggaagaggc ttttgtcgat gggcgaatta
    20221 ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga cattctcttc gtttacgggc
    20281 aaatccataa taccgttcgt tttcccaacc tgaatctcga caactccgtc cacatcacta
    20341 acctggtctt ttccatcttg cgtaacgctc gcgcgctgca tgtgggtgaa gcgccaaata
    20401 tggtggtctg ctggggcggt cactcaatta acgaaaacga gtatttgtat gcccgtcgcg
    20461 tcgggaacca gctgggcctg cgtgagctga atatctgcac cggctgtggt ccaggagcga
    20521 tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca gcagcgttac aaagacagtc
    20581 gttttattgg tatgacagag ccgtcgatta tcgccgctga accgcctaac ccgctggtca
    20641 acgaattgat catcatgccg gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc
    20701 acggcatcat tatcttccct ggcggtgtgg gtacggcaga agagttgctg tatttgctgg
    20761 gaattttaat gaacccggcc aacaaagatc aggttttacc attgatcctc accggcccga
    20821 aagagagcgc cgactacttc cgcgtactgg acgagtttgt cgtacatacg ctgggcgaaa
    20881 acgcgcgccg ccattaccgc atcatcattg atgacgccgc tgaagtcgct cgtcagatga
    20941 aaaaatcgat gccgctggtg aaagaaaatc gccgtgatac aggcgatgcc tacagcttta
    21001 actggtcaat gcgcattgcg ccagatttgc aaatgccgtt tgagccgtct cacgagaata
    21061 tggctaatct gaagctttac ccggatcaac ctgttgaagt gctggctgcc gacctgcgcc
    21121 gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt cggtattcgc gccattgaag
    21181 agtttggtcc ttacaaaatc aacggcgata aagagattat gcgtcgtatg gacgacctgc
    21241 tacagggttt tgttgcccag catcgtatga agttgccagg ctcagcctac atcccttgct
    21301 acgaaatctg cacgtaatct cccctcccc
//
LOCUS       DADKXM010000059        21265 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.059, whole genome shotgun sequence.
ACCESSION   DADKXM010000059 DADKXM010000000
VERSION     DADKXM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.059"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            15..923
                     /locus_tag="JQR05_002201"
     CDS             15..923
                     /locus_tag="JQR05_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324371.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1162..1221
                     /locus_tag="JQR05_002202"
     CDS             1162..1221
                     /locus_tag="JQR05_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY5324372.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1538..1597
                     /gene="ibsE"
                     /locus_tag="JQR05_002203"
     CDS             1538..1597
                     /gene="ibsE"
                     /locus_tag="JQR05_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY5324373.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JQR05_002204"
     CDS             complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JQR05_002204"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY5324374.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JQR05_002205"
     CDS             complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JQR05_002205"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY5324375.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SRLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6061..7362)
                     /locus_tag="JQR05_002206"
     CDS             complement(6061..7362)
                     /locus_tag="JQR05_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY5324376.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGSIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7604..8224
                     /locus_tag="JQR05_002207"
     CDS             7604..8224
                     /locus_tag="JQR05_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY5324377.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8288..9526
                     /gene="cca"
                     /locus_tag="JQR05_002208"
     CDS             8288..9526
                     /gene="cca"
                     /locus_tag="JQR05_002208"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY5324378.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JQR05_002209"
     CDS             complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JQR05_002209"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY5324379.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFTPFAIYRFIVAAAVYVVFF"
     gene            complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JQR05_002210"
     CDS             complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JQR05_002210"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY5324380.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11074..11691
                     /gene="plsY"
                     /locus_tag="JQR05_002211"
     CDS             11074..11691
                     /gene="plsY"
                     /locus_tag="JQR05_002211"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY5324381.1"
                     /translation="MSAIALGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11704..12636)
                     /gene="ttdR"
                     /locus_tag="JQR05_002212"
                     /gene_synonym="dan"
     CDS             complement(11704..12636)
                     /gene="ttdR"
                     /locus_tag="JQR05_002212"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY5324382.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPERMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            12843..13686
                     /gene="ttdA"
                     /locus_tag="JQR05_002213"
                     /pseudo
     CDS             12843..13686
                     /gene="ttdA"
                     /locus_tag="JQR05_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13683..14288
                     /gene="ttdB"
                     /locus_tag="JQR05_002214"
     CDS             13683..14288
                     /gene="ttdB"
                     /locus_tag="JQR05_002214"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY5324383.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14336..15799
                     /locus_tag="JQR05_002215"
     CDS             14336..15799
                     /locus_tag="JQR05_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY5324384.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLLIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15842..16855)
                     /gene="tsaD"
                     /locus_tag="JQR05_002216"
     CDS             complement(15842..16855)
                     /gene="tsaD"
                     /locus_tag="JQR05_002216"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY5324385.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17093..17308
                     /gene="rpsU"
                     /locus_tag="JQR05_002217"
     CDS             17093..17308
                     /gene="rpsU"
                     /locus_tag="JQR05_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY5324386.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17419..19164
                     /gene="dnaG"
                     /locus_tag="JQR05_002218"
     CDS             17419..19164
                     /gene="dnaG"
                     /locus_tag="JQR05_002218"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY5324387.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19359..21200
                     /gene="rpoD"
                     /locus_tag="JQR05_002219"
     CDS             19359..21200
                     /gene="rpoD"
                     /locus_tag="JQR05_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY5324388.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5117 a         5878 c         5470 g         4800 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
      361 ccactcgcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
      421 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
      481 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg
      541 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
      601 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
      661 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg
      721 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta gcggaacaag
      781 cattatcagc cgctctctct taccgcacac aggcaccgct gattgactcc ttgctcaatg
      841 aaattggcgt ttcaggcggt tcactggcgg cattgacttc acccttaacc tcaacaactc
      901 ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctagcac aaaaagccgg
      961 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1021 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1081 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1141 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc
     1201 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1261 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     1321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     1381 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     1441 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     1501 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     1561 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     1621 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     1681 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     1741 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     1801 gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     1861 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     1921 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     1981 aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2041 caagcggtca cccagtttgc gggcatttgc cagataggag acgtgtccgg cgtgcaggat
     2101 gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg ccgctacggc
     2161 cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc cacgcactgc
     2221 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     2281 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     2341 caggacgcca atcaccgtgt cgcctgcacc ggtaacgtca tacacttcct gcgcctgggt
     2401 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     2461 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     2521 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gtgtcagcag
     2581 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     2641 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     2701 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     2761 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     2821 aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     2881 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     2941 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3001 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3061 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3121 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3181 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
     3241 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     3301 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
     3361 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
     3421 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     3481 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
     3541 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttctcgcggc
     3601 agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg atcgccgtac
     3661 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     3721 tcggcaaatg cttctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     3781 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     3841 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc agtcatcgca
     3901 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     3961 ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc gttgaggtga
     4021 tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac ggcatctatc
     4081 atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag cgtaccggcg
     4141 atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4201 tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc atcacggtag
     4261 gcatcggtcg ccgtcggctg gtaaagggta tttggatcga gcaattcatc cagcaataat
     4321 ggataacgcg ctagccggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
     4381 ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt aacaatcccc
     4441 accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc acagacatca
     4501 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
     4561 ttatccagct ctttgcggaa atcagcaatc agcgttagga cctgtttgcg atcatcctcg
     4621 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     4681 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     4741 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
     4801 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
     4861 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
     4921 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
     4981 agcgttggca gtaaagagcg cgactgcagc gacggttcgc gcccaccgcg aatgagctgg
     5041 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5101 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5161 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5221 ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc gtaacgctcc
     5281 cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
     5341 ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc atcctgcgtt
     5401 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     5461 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     5521 tcagaggaga aattcagttc cccgccgccc agcttaccca tgcctaagat cagcagcggt
     5581 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatacagc
     5641 cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg caatatgctc
     5701 tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat gcgccgccgg
     5761 aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag cgcctcctgc
     5821 aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc cagttccgtc
     5881 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
     5941 ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc aacaacggtc
     6001 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6061 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6121 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
     6181 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6241 acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     6301 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     6361 aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta ccagccattc
     6421 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
     6481 cgcagaagcg atggtcgctt cgcattgagt cagcaaatca cgtaagtgag tgctcgcttt
     6541 acgcggcaca atgctaccga acagcatcag tgtatgacgg actaggctaa tggctgccag
     6601 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg
     6661 cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     6721 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     6781 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     6841 cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct cgcagatagg
     6901 ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc
     6961 atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg gctgcacgcg
     7021 ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta actgcgccag
     7081 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
     7141 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7201 gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7261 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     7321 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     7381 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     7441 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     7501 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     7561 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     7621 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     7681 ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
     7741 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     7801 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     7861 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     7921 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     7981 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8041 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8101 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8161 gctcggtctg gtactgccgc acctgatccc aagccgcaaa cgcaaagatc gctggatgaa
     8221 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     8281 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctatc
     8341 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     8401 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     8461 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc
     8521 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     8581 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     8641 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt
     8701 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
     8761 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     8821 gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg
     8881 cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     8941 acctgccagg tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
     9001 ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg
     9061 taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt
     9121 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9181 cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac
     9241 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
     9301 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
     9361 gcaaggccgt tggttgcgcg aagcctggga agtggcgcag tcagtgccga caaaagcggt
     9421 cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac ggcggattgc
     9481 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
     9541 gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc
     9601 agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa
     9661 cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc gcagccgcca
     9721 caataaagcg ataaatggcg aacggggtaa acgaaatgcg cttaatcaat tgcaggaagg
     9781 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
     9841 tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
     9901 tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca
     9961 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10021 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10081 gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat
    10141 acatcacatt tatcgggtta aacaatgact taatcgtgtc gtggaataat agccccagta
    10201 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttcac
    10261 cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga
    10321 acatcactac taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg
    10381 tgtcgccctc aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10441 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10501 tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga actactgcag
    10561 tatgatcagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10621 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    10681 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt
    10741 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    10801 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    10861 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    10921 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    10981 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11041 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgct tggaatgatc
    11101 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11161 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11221 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgcct
    11281 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11341 gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa aggcgttgct
    11401 accgcttttg gagcaatcgc accaattggc tgggatctca ccggagtaat ggcgggaacc
    11461 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    11521 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11581 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    11641 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    11701 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    11761 cagaaattcc acgcagacac gtaatttcat gctgcgatag agcggctccc ggtaaacagc
    11821 ccagatattg gcgctttgtg catactccgg caatacttgc accagtttgc cactctccag
    11881 aaacggcaac acatcccact cggaacgcag cataatccct ttgccctcca gcgcccattg
    11941 caacactatc tcgccgctat tggaggaaag atgcccgcta accttcaccg attttttctc
    12001 ctgaccgttc cccaactccc atattccgtg ggtcatatcg cgctctttgg tcaccagaca
    12061 gtcatgacga cttaattcct gtaatgattg tggctgcgga tatttttgca gatactcagg
    12121 tgctgcgcat aatattcttt tatttttcgt taacagatgc gcaatataat aatcaggaat
    12181 ttcgtcatta atacgaatat ccagatcaat attatcctgc actaaatcaa tttgccgatc
    12241 gaacaattca aaatgcacct gtaactcagg ataattgcgc ataagttcgg taatggctgg
    12301 cgcaatatgg ctgcgcccaa aaccaaaact gcaaccaata cgaatcatcc tctccgggcg
    12361 cgttttgatt tgcgtgacgt catcgaccag tcgctgatat tgggtgagga tttccagcgc
    12421 atgttcgtaa caacgttggc cactttcagt cagcgccacg ccgcgagcgg agcggttcag
    12481 cagcgttgtt gccagcgtat tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt
    12541 ttgcccaagc gtcgctgctg cagcactaaa actcccgctg tggacgattt ctaccagtac
    12601 ctgcaagtct ttggctaaag gccagctatt cagcatcgtt aattatccgc agttgtgata
    12661 agcgcagtgt atttcgcaaa acattgccat tccctgctct ggcgcaatat tcattcttat
    12721 tttcgcgttg attcacgaac tgttaattac atattcacgg tggcaaaaaa tataaaacca
    12781 catttttagt ggtagtttgt ggcggtgaat ttttccagac aaatacaaaa actggagttg
    12841 ccatgatgag cgaaagtaat aagcaacagg cagtgaataa gttgacagag attgtcgcta
    12901 actttaccgc catgatttct acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc
    12961 taaaggatgc cgaaacgtcg tcgatgggga aaattatcta ccatacgatg ttcgacaaca
    13021 tgcaaaaagc gattgacctg aatcgtcctg cctgtcagga caccggggag attatgttct
    13081 tcgttaaagt cggttcccgc ttcccactgc ttggcgagct gcaaagcata ctcaaacaag
    13141 ccgtggaaga ggcaaccgtc aaagcgccac tacgtcacaa tgcggtagaa atttttgacg
    13201 aagtaaacac cggcaaaaat accggtagcg gcgtaccgtg ggtcacctgg gacatcatcc
    13261 ccgacaatga cgatgcggaa atcgaagttt acatggcagg cggcggctgc acgctacctg
    13321 gccgctcgaa agtgttaatg ccgtcagaag gctacgaagg cgtggtgaaa ttcgtcttcg
    13381 aaaatatctc caccctcgcc gtaaacgcct gtccaccggt actggtgggc gtgggcatcg
    13441 ccacctcggt ggaaaccgcc gccgtactct cgcgtaaagc cattttgcgc ccgattggca
    13501 gccgccatcc caatccaaaa gcggcagaac tggagctacg cctggaagaa ggactcaacc
    13561 gtctggggat tggtccacaa gggctgaccg gcaacagttc agtgatgggc gcatcgtcgc
    13621 ggcacgctgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac ccggagcgcg
    13681 ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat attcgcgtcg
    13741 gcgatgtgat ctacctgacc ggtacgctgg tgacctgccg cgacgtttgt caccgccgtt
    13801 tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt ttccacgctg
    13861 gccccatcgt gcgcaaaaac ggcgacaaat gggagatggt ctccgtcggc ccgacaacca
    13921 gtatgcgtat ggaaagtttt gaacgtgaat ttattgagca gaccggcgta aaactggtgg
    13981 ttggcaaagg tggtatgggg ccgctgaccg aagaaggctg ccagaaattc aaggcgctac
    14041 atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtagaa gagattgaag
    14101 aagtgcactg gacagaactc gggatgccgg agtcactgtg ggtctgccgg gtcaaagagt
    14161 tcggcccgct gattgtctct attgataccc acggcaacaa cctgatagcc gaaaacaaaa
    14221 agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac gtccattaca
    14281 tcaaataacc ctcccggaga ggctcacccc tctccttttt cgcaggcata acacgatgaa
    14341 accttccact gaatggtggc gatatcttgc gccgctggcg gtcatcgcca ttattgctct
    14401 acttccggtt cccgcagggc tggagaacca tacctggctc tactttgccg tttttactgg
    14461 cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat
    14521 ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc tcgcccagcc
    14581 aggctttaag ttcaccgcca aatccctctc ctgggcagtt tctggttttt ctaattcggt
    14641 tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg
    14701 acgccgcatt gcgctgattc tggtgaaaaa gatggggcat cgcacgctgt ttctcggcta
    14761 tgcggtgatg ttctccgagc tgatcctggc acctgtaaca ccgtccaact cggcgcgtgg
    14821 tgcgggtatt atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa
    14881 cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg
    14941 cgtgaccagc gccattttcc tgacggcgat ggcacctaac ttgctgttaa ttggactgat
    15001 gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct
    15061 cagcatttta ctggttctgc tggttccctg gctggcttac gtgctgtacc cgccggtact
    15121 gaagtctggt gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct
    15181 ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc tgtggatttt
    15241 cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcgc tgatgctgct
    15301 tctgctcatt atcagttggg acgacattgt cagtaataaa gcggcatgga acgttttctt
    15361 ctggctggcc tcgcttatca ccctcgcgac cggactcaac aataccggtt ttattagctg
    15421 gtttggcaaa ctgttagcag gcagcttaag tggttattcg ccgacgatgg tgatggtgac
    15481 gttgattgtg gtgttttatc tactgcgcta ctttttcgcc agcgccacgg cgtatacctc
    15541 cgccctcgca ccgatgatga tcgccgccgc gctggcgatg ccggaaatcc cgctgccggt
    15601 gttctgcctg atggttggcg cggcaattgg tctggggagc attcttacac catacgccac
    15661 cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact
    15721 gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat
    15781 gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt
    15841 tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg
    15901 ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag
    15961 tacaaaattc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca
    16021 gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc
    16081 ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa
    16141 aggcgcgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg
    16201 ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac
    16261 gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc
    16321 caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat
    16381 cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat
    16441 gaccgccgga caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg
    16501 gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg
    16561 ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg
    16621 tataggccac agcatcaatg tcttttgccg ttaaaccaga ctcctttagc gccgcctgga
    16681 tcaacggtac ggttttacgc acatgatcgc gggaggccag ttcaggcacg acgccgccgt
    16741 agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt
    16801 cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt
    16861 gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg
    16921 ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa
    16981 aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat
    17041 aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt
    17101 aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg
    17161 cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac
    17221 cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga
    17281 aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg
    17341 agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat
    17401 tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg
    17461 gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag
    17521 aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac cgttaacggt
    17581 gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcctg
    17641 atgaactacg acaagctcga gttcgtcgaa acggtcgaag agctggcagc aatgcacaat
    17701 cttgaagtgc catttgaagc aggcagcggc cccagccaga tcgagcgcca tcagaggcaa
    17761 acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt acaacaacct
    17821 gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgct
    17881 cgctttgcga ttggttttgc gccccccggc tgggacaacg tcctgaagcg gtttggcggc
    17941 aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcaggga
    18001 cgcagttacg atcgtttccg cgaacgggtg atgttcccca ttcgcgataa acgcggtcgg
    18061 gtgattggtt ttggcgggcg cgtgctgggc aacgataccc ccaaatacct gaactcgccg
    18121 gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat
    18181 aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt ggcgctggcg
    18241 caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata
    18301 caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc
    18361 cgcgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga cggccgtcag
    18421 ctacgcttta tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg aaaagaaggt
    18481 aaagaagcgt ttgaagcgcg gatggagcag gcgatgccac tctccgcatt tctgtttaac
    18541 agtctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg
    18601 gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa
    18661 ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg
    18721 gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt
    18781 atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg
    18841 gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc
    18901 cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc
    18961 acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc tgagcaaacc
    19021 ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta
    19081 atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta
    19141 aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg atcgggaagc
    19201 ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt
    19261 tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc
    19321 caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac
    19381 agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca
    19441 atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga
    19501 tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg
    19561 ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg
    19621 aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg
    19681 ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca
    19741 accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt
    19801 acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc
    19861 cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg
    19921 aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg
    19981 atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaacta cgcgctcagt
    20041 acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag
    20101 agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact
    20161 acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca
    20221 tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc ctgtttaccg
    20281 gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag ccgtggtcgg
    20341 aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcaaaaactg cagcagattg
    20401 aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg
    20461 gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta
    20521 tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag
    20581 gtaacatcgg cctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct
    20641 ccacctacgc aacctggtgg atccgtcagg cgatcacccg ttctatcgcg gatcaggcgc
    20701 gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc
    20761 gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct gaacgtatgc
    20821 tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg
    20881 aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc
    20941 tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg cacgacgtgc
    21001 tggctggcct gaccgcgcgt gaagcaaaag ttctgcgtat gcgtttcggt atcgatatga
    21061 acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc gaacgtatcc
    21121 gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgttgc
    21181 gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacta
    21241 gtctctctgc acaaacgcca ccttt
//
LOCUS       DADKXM010000060        20781 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.060, whole genome shotgun sequence.
ACCESSION   DADKXM010000060 DADKXM010000000
VERSION     DADKXM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.060"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..808)
                     /locus_tag="JQR05_002220"
     CDS             complement(<1..808)
                     /locus_tag="JQR05_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5324389.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERMNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVL"
     gene            complement(822..1481)
                     /gene="pgmB"
                     /locus_tag="JQR05_002221"
     CDS             complement(822..1481)
                     /gene="pgmB"
                     /locus_tag="JQR05_002221"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY5324390.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNTPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1478..3745)
                     /gene="ycjT"
                     /locus_tag="JQR05_002222"
     CDS             complement(1478..3745)
                     /gene="ycjT"
                     /locus_tag="JQR05_002222"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY5324391.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGYVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3742..4797)
                     /locus_tag="JQR05_002223"
     CDS             complement(3742..4797)
                     /locus_tag="JQR05_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY5324392.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAWEMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQLIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4807..5595)
                     /locus_tag="JQR05_002224"
     CDS             complement(4807..5595)
                     /locus_tag="JQR05_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY5324393.1"
                     /translation="MKIGTQNQAFFPENIRERFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5613..6665)
                     /locus_tag="JQR05_002225"
     CDS             complement(5613..6665)
                     /locus_tag="JQR05_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY5324394.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6696..7538)
                     /locus_tag="JQR05_002226"
     CDS             complement(6696..7538)
                     /locus_tag="JQR05_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY5324395.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7525..8406)
                     /locus_tag="JQR05_002227"
     CDS             complement(7525..8406)
                     /locus_tag="JQR05_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY5324396.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8427..9710)
                     /locus_tag="JQR05_002228"
     CDS             complement(8427..9710)
                     /locus_tag="JQR05_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5324397.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNRQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLASLTITAGQKTEAAEKFVTFMEQADNIADWVMMSPG
                     AALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDVTGS
                     GVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9724..11401)
                     /locus_tag="JQR05_002229"
                     /pseudo
     CDS             complement(9724..11401)
                     /locus_tag="JQR05_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11613..11960)
                     /gene="pspE"
                     /locus_tag="JQR05_002230"
     CDS             complement(11613..11960)
                     /gene="pspE"
                     /locus_tag="JQR05_002230"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY5324398.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(12003..12224)
                     /gene="pspD"
                     /locus_tag="JQR05_002231"
     CDS             complement(12003..12224)
                     /gene="pspD"
                     /locus_tag="JQR05_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY5324399.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12233..12592)
                     /gene="pspC"
                     /locus_tag="JQR05_002232"
     CDS             complement(12233..12592)
                     /gene="pspC"
                     /locus_tag="JQR05_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY5324400.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12592..12816)
                     /gene="pspB"
                     /locus_tag="JQR05_002233"
     CDS             complement(12592..12816)
                     /gene="pspB"
                     /locus_tag="JQR05_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY5324401.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12870..13538)
                     /gene="pspA"
                     /locus_tag="JQR05_002234"
     CDS             complement(12870..13538)
                     /gene="pspA"
                     /locus_tag="JQR05_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY5324402.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13705..14682
                     /gene="pspF"
                     /locus_tag="JQR05_002235"
     CDS             13705..14682
                     /gene="pspF"
                     /locus_tag="JQR05_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY5324403.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14802..16067)
                     /gene="puuE"
                     /locus_tag="JQR05_002236"
     CDS             complement(14802..16067)
                     /gene="puuE"
                     /locus_tag="JQR05_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY5324404.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(16105..17385)
                     /locus_tag="JQR05_002237"
     CDS             complement(16105..17385)
                     /locus_tag="JQR05_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY5324405.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(17387..18874)
                     /gene="puuC"
                     /locus_tag="JQR05_002238"
     CDS             complement(17387..18874)
                     /gene="puuC"
                     /locus_tag="JQR05_002238"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY5324406.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(19024..19581)
                     /gene="puuR"
                     /locus_tag="JQR05_002239"
     CDS             complement(19024..19581)
                     /gene="puuR"
                     /locus_tag="JQR05_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY5324407.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19608..20360)
                     /gene="puuD"
                     /locus_tag="JQR05_002240"
     CDS             complement(19608..20360)
                     /gene="puuD"
                     /locus_tag="JQR05_002240"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY5324408.1"
                     /translation="MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHA
                     LAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALER
                     RIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
                     GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWH
                     PEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            20584..>20781
                     /locus_tag="JQR05_002241"
     CDS             20584..>20781
                     /locus_tag="JQR05_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324409.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEK"
BASE COUNT         4853 a         5662 c         5224 g         5042 t
ORIGIN      
        1 tcagaaccgc taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat
       61 cgccatcgat cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa
      121 acatattcgc gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt
      181 ctttcataat cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt
      241 agatcatggt ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgtacgc
      301 gcagcttggc atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca
      361 caatcgcccg cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc
      421 gtttcaggta ctcacgcagg ccgagaattt gcgccgccca gttcatccgc tcatcaatca
      481 cctctttggc gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat
      541 gcggatacaa cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt
      601 cattcattcg tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa
      661 tcatgcgcag ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt
      721 tatcggcaat ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt
      781 gaatatgttg taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag
      841 gccgataacc gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt
      901 aagcccgcac cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc
      961 tgcgcatctt caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg
     1021 agaaagattt ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg
     1081 aaaaactcgc gcagctccag cgccgctaaa atcgtcggcg tattcaggga gacggaagcc
     1141 agccctaccg gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc
     1201 acagcattaa ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc
     1261 agttgcgccc gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc
     1321 cgcagagact catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg
     1381 atgccaattt cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg
     1441 attacaccat ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg
     1501 tagcggtccc attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca
     1561 cggatataag ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac
     1621 gctgcgcgtc gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact
     1681 gttgccactg ctcaggtaac gccggattta gatgcaattc accgtcacgc acgctcaccc
     1741 cggcaaaacc ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat
     1801 catcacaact atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat
     1861 aactttgggt cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg
     1921 acgagtcgtg aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg
     1981 agaactgctc tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca
     2041 tctcgttcac ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt
     2101 tgtatttcgc cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac
     2161 cgtcgggctg aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa
     2221 acgcgtcgtc actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat
     2281 acaccatata gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa
     2341 taacatcatg aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg
     2401 cagtttccag aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct
     2461 gccagtattg aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg
     2521 aggccacttt ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt
     2581 cttcgccgct gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg
     2641 ctttctcctg cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg
     2701 tcggatcgct aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtagc
     2761 ctttgtagcc ttcgccggtt aagccttttg ccgcaatact gctgcgctcg tcgtgagccg
     2821 gcgtcatgat gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc
     2881 cgccatttac cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg
     2941 caagatgttg gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc
     3001 cccactcgtc taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca
     3061 acgtcactgt ctcgcctgca tgaagctgcg cactggtatg ttgcagtaaa cggcgctctt
     3121 tggcggtata gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat
     3181 cactgcgccc atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca
     3241 cctgggtttc gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg
     3301 aaatcagcac tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc gcaatgagcg
     3361 gcagttggtc tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg
     3421 tacgccagac aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg
     3481 cttcgcggga taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag
     3541 gcagattcac cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat
     3601 acatccctcg cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc
     3661 cgttcccctg cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt
     3721 ctgataacgt tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata
     3781 taacgccgcc accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg
     3841 cttgccctgt acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc
     3901 catttcccgt tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg
     3961 aaacagcgtc gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg
     4021 gatgttgagt gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt
     4081 gccaaacagc gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca
     4141 gctcttttgc gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa
     4201 gcccagcaca tacatcgcag catccagcat atgaatgccg atgtcgatca gcgggccacc
     4261 accctgcagt tctttattgg taaagacacc ccagccggga acgccgcagc ggcgcagggc
     4321 gcgggcggtg gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg
     4381 ttgcgtatcg agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg
     4441 cgcggtatcg cacatttccc acgcctgttc tggcgtcatg gcgggcggtt tttcgcacat
     4501 cacatggcag cctgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga
     4561 gcagacgcta accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac
     4621 ggatgcattc ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac
     4681 agccaccagt tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac
     4741 ctggcccgcg cctattatcg cgacccgcag cggagagctt gtcattgcac ttttcacaaa
     4801 gacctcttag caggtacgca accaggccaa cgaatcacgg tacgcctggg caggatcttc
     4861 tgcccggata cgcccttcat acaccacata accctgatag ttatcggcgc gcagctgttc
     4921 aaacagcgcg tggaaatcga gggtgccact gcccggctgg taacgatggt tatcggcaat
     4981 atgcacatga ccgagcaagt cacggttgtc gtgcagcgcc tgcgccaggt tatcttcttc
     5041 gatgttcatg tgatagaaat cgccgataat ctgcacatgt ttaagattgt tttcgacgat
     5101 gtaacgacga gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggttc
     5161 gagatacacc acggttccgg tacgcgcggc gacctgttcc agtacgcgca gggaatcact
     5221 caccattttg cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt
     5281 aaacatgccc cacgctgccg gaacgacgat acctttaccc cccacttctg ccagcgcctc
     5341 gagaatgcgt tcgatctgct ttaagccatt aagacgacgc tcttcgataa agtcaccaat
     5401 ccagccgtca tagccgccgc aggcggtggt cactggtaaa ccggtttctt tgatcgccgc
     5461 tttgacttct tcgatgttgt tgaccagcag tttgccgtca atctcaaaac catcgaagcc
     5521 catctctttg atataacgaa atctctcgcg gatattttcc ggaaagaatg cctgattctg
     5581 tgtgccgatt ttcatttttt tattcctttg aattaaaacg taacgcccat tttgatgctc
     5641 tgttccggat gctgatcgac atactgcatg tagctttccg ggctgttggc aaaggtcact
     5701 accgggtcga ttaaatcttc gcaattgaga taaccgttca tcagcagttc ccagcaggtt
     5761 tcttcaatac gcttacggct ccagcgcgga taatccgggt tcggttcgct gcacgcgcgg
     5821 gagaaaacaa ttttggcgtt attgaaatgc gcttcgcgtc cgaggttaaa accttcagca
     5881 aatggtttgg caaacgcgac ataggagatg gtgccgccgt aggccaggcc gcgcagcgcc
     5941 gattgcagcg cgtcggcgta accgctggtt tcgataatca catcggcccc ctgcttgccg
     6001 gtcagcgttt tgatctcttt gcctacgtca gtgccaatgg ggttaaggca gaaatcagca
     6061 ccgtggcgac gggcaatatc acagcgatgg gcgataggat cgacgccaat taccaccgat
     6121 gcgccagcgc gtttcgccag ttggatggca atttgaccga tcgcgccaag ccctaccacc
     6181 acgacaaaat cccctacgcg tacgttggca tcgcgcacgc cgctcatggc aaactgcgcc
     6241 gggtcgtaac agacggcgtt tttccaggag ctgccttgcg gcattttgcg cagcttgtag
     6301 ttattcactg cgttaatgat gaccgtctcg gagagcgggc cgtagccgca tacgctgtcg
     6361 cccaccgcgt agtctgtaac gtcgctgccg cactcgataa tgtcgccaac caccatgttg
     6421 ccaagctgga atttgccaaa ctcaatgccg cgcggcgcat ctgccggacg cggggtgaac
     6481 atctgccact cgccgttaaa gtcttcatcg ataaacgggc tggcggcgcg gaagtcgacc
     6541 acttccgttc cgtgtttcgg tgcgccgaaa cgcacgcgga tcttcacttc attggctaaa
     6601 atcgcccgat cttcatactc aaccagcgca gcaacacgcg gtgctgtggc tactaacttt
     6661 ttcatgtaaa actccttgta aagaaactgg ccgctttagc ccttcacgcc accggcggtc
     6721 aaaccactct taataaaacg ttccgaaagc gcatacataa tcaccaccgg caatgcggtc
     6781 accagcgagg ccgccatcat ccgtccccag atgtaatcag gcgtactgaa cagcgcgttc
     6841 aggcccactg gtaaggtgaa attgctggcg ctggagagga aaatcgacgc aaacagatag
     6901 tcgttccacg ccaccataaa gcagtagacg aacaccgata tcagcccgga catcgccagg
     6961 ggcacggtga tacggaagat gatttgcaga cggttgaggc catccatcat cgccgcttct
     7021 tcgatctcat cagggatggt gtcgaagtaa cttttcagca taaacacggc ggtgggcagg
     7081 gtttgcgtca ccatggtaat gatcagcgcc atctcggtgt cgtaaatacc aagcgcggtg
     7141 atgattttga acagaggcac caccagcaaa atgccagaga acatatacac cgtgtaaaag
     7201 ctggcgttga tggtcatccg gcctttaaag cgcaggcggg aaagcgcgta agctccgaga
     7261 atgccgagaa ataccgccac cacggaggaa accaccgaca ccaccatgct gttacggaag
     7321 tagtcgacaa acgggaaaat catcgggtta aaaatgtcga cgtaatgctc cagcgtccac
     7381 tgttgcggca gcagcgtagg atgcagcgat atcgcctctt tcgcgctttt gaacgaggtc
     7441 atcagcatca caaaaaacgg aaacagcgtg atgatgagaa acagcgccag cccgcagtaa
     7501 aaaccgatac gactgagcgt gcgtttattt gttgccattg aggttcaccc gtttcctggt
     7561 cagcagaatg acagcgaaaa taatgatgaa gagcaccacc gagatcgccg ccgctttccc
     7621 taagtcatta aaggcaaagg cggttttgta gagatagaca ccaagaatat cgaccttggt
     7681 tgtcagcaga taaacatcag cgaacatgta gaacatccag atggtgcgca gggtcaccac
     7741 cgtcgccagg accggcataa ttgcgggcag cgtgacgatg cgaaaccgct gccaggcatt
     7801 agcgccatcc atctctgccg cttcgtacag cgatttgtcg atggtctgca aaatcgccag
     7861 aaacgagata aaggcatacg ggaagtagcg ccagatggcg aacagcacca ccagcacaaa
     7921 gctactgccc ggattatcaa accacagcgg tgcctgctca tagagatgca gaagatcgac
     7981 gccgaggtag ttcacaatgc cgtagccgtt gttgaacatg tatttccagg caaacaccag
     8041 cgaaatggac ggcgttacgt aggagaggat caccagcgaa cgcgcggttt tgcgcaggcg
     8101 gaactcgcgg ttgaaaaaca tcgccacggc cagcccgagc acggtgctgc ccgccaccac
     8161 cagcgccgta taccagaccg tcatccacag cgagtgccag aagccgggat cggagagaat
     8221 gcgcacatag ttactcaccc caacaaacgt tgactcgatg ttgggattga gcggcagacg
     8281 taaaaaactg atttcgatat ttgacaccat cggccacgcc accagaccgc ccagcagtaa
     8341 taagctgggc gcgagaagca gcagcgcaaa cggcatatcg gaacgacctg aaaaaagcct
     8401 gttcattgcg gattcctgtc ctgggtttag tgctgttcga tcagttcatc cagctttttc
     8461 tggctcgctt gcagcgtgcc agggagatcg gctttaccca cggtgacgtt atgcaccatg
     8521 gagctcacca caccagaacc cgtcacatcg cccatgcggg taaagttttt atcccctact
     8581 gcgccaaaga cctgaatatt tggcagttca ccgattagct gattcggtag ttcacccagc
     8641 gccttaataa cgtcgttgtc tttccaggtg gcggtagtga caaccgcttt attcaccggc
     8701 agcgcagcac ctggcgacat catcacccag tcggcaatgt tgtccgcctg ctccataaag
     8761 gtgacaaatt tttctgctgc ttcggtcttt tgtccggcgg taatggtcag cgaggccaac
     8821 atgccgtaga ccgcagagtt tttctcggtt ggcaccacga aaccgacgtt tttcgggtcg
     8881 ccttctttaa tcacagccgg aaggatatag gtggagtaaa tcgccatcgg cgcggtgccg
     8941 ttcataaagg cgtctttcac ttccatgatg tcgttagaac ccggcatggt gttggcagca
     9001 aggtcgcggt aataggtcag cgcctgcatc atctctggtg tatcaagggt gattttgcct
     9061 tcggcgttaa agacgttggc ctggttggat aacgcaaact gggaaaagga ttgttccgtc
     9121 agcacgcttt ctgccgtagg cagcgcaata ccgtattttt tattcgccgg gtcattcagt
     9181 ttctgtgcaa cgtccagcag ctgttgcctg tttttcggct cctccagccc cgcttttgcc
     9241 agcacatctt tgcgatacca gatgccgcca atccaggcgc tgacaggaac accggtccat
     9301 gcgctaccat cttcggtacg cacaatacgc agtacgccat cgtaaaacgc gccttcacca
     9361 acgttgctga tgaccgtggc aaccgctttg cgatcgataa gctgctcttt atccataact
     9421 ttggcgtagt catggctggt ttcgatcact tccggcagcg agccgctacg tgaaagggtg
     9481 atgactttgg tgttataagc gtcttcttcc acgggcactt gtttaacggt gatacctggg
     9541 ttttcttttt caaaacgggc aatcaatttg ctgataactg cctgacgctc ctgctccacc
     9601 gaagaatgca taaattcgat ggatacatgg gttttatttt cttctttaca gcctgaaatc
     9661 agggcgcagg aaaccagtgc tgataacagc acgatttttg atttaatcat gagatgttcc
     9721 tttttattta atccacataa cctgccaggg gcgcagcgtt atttcattac ccaatatatc
     9781 aacttcacta atcagatccc gaccatgcag atcagtaata tttacatgct gaatattttt
     9841 actgacatta aacaatccag tcagacaatt accatcagcg ttacttcttt gaatacgcat
     9901 tacggatgaa ttaaccgtat cgatggtaaa attattatcc ggatgaaact cgttatggct
     9961 gcgacgaagt gtaattaaac gcgacaactc atgatatacc gcatgcctta atgtagcttc
    10021 atcgttcagt tctcgtgtta tctcttcact gtgatatttt ttacggttaa tcgcacggtt
    10081 atatccgagt ttttcgacac ctgcgtaatc attacgcgag ccaagaatac tttgaatata
    10141 tatcgcgggt acaccgggga aacttaacaa aatcgcatgg gcaaggataa acctggcgca
    10201 acgttcttca tcgctactct cgcggcggct taacgcatcc atataggtga cattcatctc
    10261 atagggactg cgcgtgccgt cgggattatt tttccagttt actaatgcac cttcctgctg
    10321 taatgcctcg accagcgcta atatttcgct ttctggaagc aagccacgca gtgggttcaa
    10381 cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtc gttttactgg agggcaagct
    10441 cagactttgc gcccactggc acagcgcctc gacgttctgt ttttgcaccg catgtaacac
    10501 cagcggcggc agcgagaact ggtacaccat atgcgcttcg tcatcaccgt tgccaaagta
    10561 agcaatgttg tctttgtgcg gaacattggt ttcggtaatg atcactgtac ctggtgctac
    10621 gtcatcaata atcgaccgta acagcttgat aatcagatgt gttttttcca gatggatgca
    10681 gcttgttccc ggctctttcc acataaagcc aacggcatcc aggcggacat actcagcacc
    10741 tttctcaagg taacagagta aaacatccac catcgccagc aacacttcag ggctacggta
    10801 attcaggtca atttgagtca ctaaaggtgg tccataaatg gcgcgtggaa ttatcatcca
    10861 tttgaaacgg cgttaatagc ggtaacgctc gcggacgggt gacggcactg agatcggttt
    10921 tcggatcaac cgcaataaag aaatcttcaa aacccggaag ctgttgtaaa tagtttttaa
    10981 accattcact ttttgcagac atatggttgc agacaaaatc aaacattaaa tgactgcatt
    11041 caccgagttg ctgaatatcc tgccactccc ccgcttcact ggcgacctga tgataatcaa
    11101 ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg taataaatga acatgtgaaa
    11161 aaatgctttg cagccattga tggtagaact gattaaatat gggtagtggt tttaaatcat
    11221 tactgtgaaa ttgatcggca taggtaatta acacgacatc actttcatcc cagtgttttt
    11281 tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat
    11341 gagttgcggt aaatgttcca ccgtagattt cgtccaggta atccgtaatt ttctgtttca
    11401 tgttgccttt ccgtggtagc ggtcccatga agcgagagac tactccgcag aaaataaact
    11461 gtcaattgag cacaagggtt ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg
    11521 tatttgtgag ctgacgcaaa taatctatag ggatgtaaat aaggtgtgac agaaaaaaaa
    11581 cggcgcataa gcgccgctca tggtgaattc ttttaacctt tgaccttcgg cattgcgatg
    11641 tctttcaggc caccggcgtt ctcaacgtgg gtatatccca tctcgctaag gatctctttt
    11701 gcctgcccgg actggcgtcc ggcattgcaa tacactttca cggtgtcgtt tttatccgga
    11761 acggcggtgg caatgcgctc tttcacttcc ttcaggggaa tattgatggc cccctgaacg
    11821 tgctcttgct gatactgctc tggaacacga acatcgatcc agtgttcagc ggcgaaaacg
    11881 ggcagtgaaa acaccagtgc cagagcaagt aagccttttt ttaaacatgt aagcgtcctt
    11941 ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt gcgaaactcc
    12001 cctcaccttt tataacgctg tgccagttta ttagcagccc gacttaacag cggttccagc
    12061 gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc ccagcccgcc
    12121 acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa
    12181 ccaggcttca ccttttgccc ggcctgttgc cagcgagtat tcataacctt cctcacagtt
    12241 gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta
    12301 aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg ctgctggaag
    12361 gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga
    12421 tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac
    12481 gcaccagttt taccggtaca tcaaaatagt tggcaatccc ggcgcagacg ccgcggacca
    12541 tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc
    12601 cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac gttcgcgcat
    12661 ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact
    12721 gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa
    12781 aatggttaac ggaatagcca gaaatagcgc gctcataagt actccttaga cgatgagcgg
    12841 cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
    12901 aattgtgcca gttgttcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc
    12961 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaatacga
    13021 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga
    13081 cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca tcaatgcctg ctggcgagcg
    13141 cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt tcttcatgcg tgccagcgta
    13201 tcgtccacca gcgtcacttc atgttccagg gacttaatca gatcggtcag tttctgtttt
    13261 tcaattaacg ctgcacgtgc cagatcctct ctctctttca gcagcgccag ttcggctttt
    13321 tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt
    13381 tctgccaacg cacgcgccga agtagaacgt acttcaacca gtgtatcttc catctcctgg
    13441 atcatcagac gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg
    13501 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg
    13561 cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt ttatcttttt
    13621 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat tagcgaatta
    13681 cactaacaag tggcgaattt catcatggcc gaatacaaag ataatttact tggtgaggcg
    13741 aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg
    13801 ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg ccagccgcct gcattatctc
    13861 tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
    13921 ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat
    13981 cctgggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca
    14041 ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt acggtgaact ggagcgcgtt
    14101 ggcggtagcc agccgttgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc
    14161 ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc tcgaccggct ggctttcgat
    14221 gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat ggcagaacac
    14281 tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc
    14341 gccagagaaa cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt gaaaaacgtg
    14401 gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga catcattatt
    14461 gatcccttta aacggcgtcc acctgaagaa gccatcgccg tttcagaaaa tacctcgctt
    14521 ccgacgctgc cgctggattt acgcgagttt cagatgcagc aggaaaaaga gttgctgcaa
    14581 ctcagtttgc aacaggggaa atataaccag aagcgcgcgg cagaattact gggtttaact
    14641 tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg agcaatacaa
    14701 cttgccggat gcggcgtgaa tgctttattc ggcatacata atcaatacgt cgtttgcagg
    14761 cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct cagcgcatcc
    14821 tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaag
    14881 cgaatcacgt tgccgtatgc gccacaggtc agcaggagca gcccctgcgc cagcgcgcgt
    14941 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
    15001 gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg ccggaacgct ttctttggca
    15061 tcaatcaacg tgtttttgag acgctggccc agttgattcg cgcgttcgca gagtgattct
    15121 ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag
    15181 gtgccaccca acccgcccgg cgcgggcgcg tccataatat tcgcgttacc gaccacgccc
    15241 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg
    15301 taatgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac ttcatcggca
    15361 atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc
    15421 gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc
    15481 gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg
    15541 ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg
    15601 attttgtacg gcgcaacttt tccggtcagc gccatggtca tatatgtacg accgtggaag
    15661 ccaccgctaa acgcaatcac accaggtcgt ccggtatggg cgcgagcaat tttcaccgcg
    15721 ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt tagcctgccc gctcactggg
    15781 gcaagggcgt taattttctc cgccagcgtg acgtagcttt cgtacggcac aatctgatac
    15841 gccgtgtggg taaactgttg caattgctgc tccaccgccg cgaccagatc agggtggcga
    15901 tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtattcgtt gccctctacg
    15961 tccttcagcg tagcgttttc tgccgactgg gcgaagaagt tacacatcac gccaaccccg
    16021 cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
    16081 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc
    16141 cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg
    16201 cagattggcg aaggcatcga aacgctcggc gtcaccgcgc agcagttcag caatcaaacg
    16261 cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt
    16321 ggtatcaagg cggccgaact gcggcatgcg cgacaaggtc agcaggaagt tgcccgtcca
    16381 gcggtaatca attttcacgc ccttcaactg agggaaggtt tttagtagtt tcggtaccac
    16441 aagacgctca acgtcatccg ggttgcgtgc accatacact acacccccgc cgtacagcag
    16501 gcggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
    16561 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg tgatcacctg
    16621 cgtgccgcac ggcatgctgc gtttcgctag ttccggctct actttatcgc ccagatacgc
    16681 attcccggcg acaatcacat acttcgccgt cacctgacca ttggcagtcc gaaccacggc
    16741 tggcgtggtg tgctggatct gcgtcacggc ggaaagctca tacacgcgcc cgccgttcag
    16801 gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgcc caccgctgtg
    16861 atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc
    16921 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt
    16981 ggcgagttgt ttatcattca tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat
    17041 ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
    17101 catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga
    17161 gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag ccgatgcgtg aggcttcgag
    17221 aaccactacg tcaaagcccg cttccgccag atgcagcgcg gaggagagcc cggtatagcc
    17281 gccgccaacc acgcaaacgt cgcaggtgat tgactcattc agtgtgtcga atggtgcata
    17341 tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc
    17401 ttatccagat ggttttcagt tcagtgaatt tttcaagggc atgcagggat ttgtcgcgac
    17461 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
    17521 tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc
    17581 gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgc agcgcctgat
    17641 cttctgatgt gaaacgggtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
    17701 acgcattcgg gtccacatcc acgaagatgg tcgggccgat ggccgctgcc agcccggcgt
    17761 tgcgaccatc cagcagcagt tgccccttgc cttcgccttc acgaataaag gtgtgtaccg
    17821 agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc
    17881 ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct
    17941 cttccagtaa caaacgcgtt ccggcgatgc acacctggcc ctggttgtag aagatgcctg
    18001 cggcggtggc gcttgccgct ttttgcagat ccggacaatc ggcgaaaacg atgttggcgc
    18061 ttttgccgcc cgcttccagc cagacacgtt tcatgttgct atcacccgcg tctttcagca
    18121 gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc atcgatatcg ttatgacgcg
    18181 aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca
    18241 agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg
    18301 gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca
    18361 acagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca
    18421 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg
    18481 cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg
    18541 gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat
    18601 cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
    18661 gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
    18721 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg
    18781 ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt
    18841 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
    18901 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc ccgtttattt
    18961 ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    19021 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
    19081 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
    19141 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
    19201 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
    19261 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
    19321 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    19381 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    19441 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    19501 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    19561 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    19621 ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat acgcgaaagc
    19681 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    19741 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    19801 ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt
    19861 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
    19921 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    19981 ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg gcagatggcg
    20041 aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag aagatcacgc
    20101 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    20161 ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt
    20221 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    20281 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    20341 ccgataaccg gattgttcat tatattttcc acgctcaatc tcacaaagtg gactaaatta
    20401 tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    20461 ttttcatttt tgaaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg
    20521 aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    20581 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    20641 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    20701 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
    20761 agcctgaaga agctcgagaa a
//
LOCUS       DADKXM010000061        20696 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.061, whole genome shotgun sequence.
ACCESSION   DADKXM010000061 DADKXM010000000
VERSION     DADKXM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.061"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..1870)
                     /gene="torS"
                     /locus_tag="JQR05_002242"
                     /pseudo
     CDS             complement(1..1870)
                     /gene="torS"
                     /locus_tag="JQR05_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            1998..3026
                     /gene="torT"
                     /locus_tag="JQR05_002243"
     CDS             1998..3026
                     /gene="torT"
                     /locus_tag="JQR05_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY5324410.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLECHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(2999..3691)
                     /gene="torR"
                     /locus_tag="JQR05_002244"
     CDS             complement(2999..3691)
                     /gene="torR"
                     /locus_tag="JQR05_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY5324411.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            3821..4993
                     /gene="torC"
                     /locus_tag="JQR05_002245"
     CDS             3821..4993
                     /gene="torC"
                     /locus_tag="JQR05_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY5324412.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSSDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            4993..7533
                     /gene="torA"
                     /locus_tag="JQR05_002246"
     CDS             4993..7533
                     /gene="torA"
                     /locus_tag="JQR05_002246"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAY5324413.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPDGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            7530..8129
                     /gene="torD"
                     /locus_tag="JQR05_002247"
     CDS             7530..8129
                     /gene="torD"
                     /locus_tag="JQR05_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY5324414.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQVALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAALCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(8222..8527)
                     /gene="cbpM"
                     /locus_tag="JQR05_002248"
     CDS             complement(8222..8527)
                     /gene="cbpM"
                     /locus_tag="JQR05_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY5324415.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(8527..9447)
                     /gene="cbpA"
                     /locus_tag="JQR05_002249"
     CDS             complement(8527..9447)
                     /gene="cbpA"
                     /locus_tag="JQR05_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY5324416.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPSSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            9708..10964
                     /locus_tag="JQR05_002250"
     CDS             9708..10964
                     /locus_tag="JQR05_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY5324417.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYVYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            11257..12498
                     /gene="agp"
                     /locus_tag="JQR05_002251"
     CDS             11257..12498
                     /gene="agp"
                     /locus_tag="JQR05_002251"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY5324418.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(12536..12763)
                     /locus_tag="JQR05_002252"
     CDS             complement(12536..12763)
                     /locus_tag="JQR05_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324419.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(12784..13380)
                     /gene="wrbA"
                     /locus_tag="JQR05_002253"
     CDS             complement(12784..13380)
                     /gene="wrbA"
                     /locus_tag="JQR05_002253"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY5324420.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            13753..13926
                     /locus_tag="JQR05_002254"
     CDS             13753..13926
                     /locus_tag="JQR05_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY5324421.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(14008..15336)
                     /gene="rutG"
                     /locus_tag="JQR05_002255"
     CDS             complement(14008..15336)
                     /gene="rutG"
                     /locus_tag="JQR05_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY5324422.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(15357..15851)
                     /gene="rutF"
                     /locus_tag="JQR05_002256"
     CDS             complement(15357..15851)
                     /gene="rutF"
                     /locus_tag="JQR05_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY5324423.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     SVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(15862..16452)
                     /locus_tag="JQR05_002257"
     CDS             complement(15862..16452)
                     /locus_tag="JQR05_002257"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY5324424.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(16462..17267)
                     /gene="rutD"
                     /locus_tag="JQR05_002258"
                     /pseudo
     CDS             complement(16462..17267)
                     /gene="rutD"
                     /locus_tag="JQR05_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            complement(17275..17661)
                     /gene="rutC"
                     /locus_tag="JQR05_002259"
     CDS             complement(17275..17661)
                     /gene="rutC"
                     /locus_tag="JQR05_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY5324425.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(17673..18365)
                     /gene="rutB"
                     /locus_tag="JQR05_002260"
     CDS             complement(17673..18365)
                     /gene="rutB"
                     /locus_tag="JQR05_002260"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY5324426.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(18365..19456)
                     /gene="rutA"
                     /locus_tag="JQR05_002261"
     CDS             complement(18365..19456)
                     /gene="rutA"
                     /locus_tag="JQR05_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY5324427.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIIARMAA
                     TIDSTSGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            19744..20382
                     /gene="rutR"
                     /locus_tag="JQR05_002262"
     CDS             19744..20382
                     /gene="rutR"
                     /locus_tag="JQR05_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY5324428.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         5091 a         5287 c         5444 g         4874 t
ORIGIN      
        1 tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc
       61 ttacctgcac aaatggctgg aagatttctg ccagtttcgc gggatcaata ccgcagccgc
      121 tgtcttccac ttcgaccagc cattgctcgc catcagtgcg actacgcagg ataatgtgcc
      181 cttcgtcagt aaaacgcagg gcattgctca ataggttggt aatgacctga cgaatacgtc
      241 gtggatcgcc cattaacgcg ctcggtacat catcggcaat tgccgttgcc aggcgaatcg
      301 ggcgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcagc gggcgcggtt
      361 caaagggctc atcgctgact gaaacatttt tgccacctgc ttcgatagcc gaataatcga
      421 gaatatcgtt gaggatggtc agcaacgatt cgccagagtc ggtaatcgcc cgcaaatcat
      481 cacgctgggc gttaagtgcg gggttatctg ccagcagttg agcagtgccg agaataccgt
      541 acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt gcctagctgg
      601 ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg
      661 tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg attcagtgca tgaacactgc
      721 tgcgaaacgc atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg
      781 ggaaagggga gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa
      841 gtggacgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt gcgcaaagtg
      901 aaaccatccc cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgtg
      961 ccagtccatg ctgattgcgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa
     1021 actgcgcgaa ctgggcgata ttattttggg cgagagtttg caggtgattg ctgatttcac
     1081 tgtcctgctg gaacagcgcc aacagatcgc tatattggct aacggtagtt aacgttgttg
     1141 cgacctgcgc acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca
     1201 ccgcattatt gagctgcttt tccagcgttg gtgcattttt ctggagctgc tccagcccca
     1261 gattcatcac catttgctgc acccgcagag cgctaaggcg cagttcattc atctggttaa
     1321 catactcaag atcgatatca atcagccgat cgagtgcact ttcagcagcc tgacgctgat
     1381 cttgttcgat caaatcgtaa atcccggcct gggtcgctcc agcggaagtt gtcgcattat
     1441 tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc agctatctgc tgactgagtt
     1501 gccgttgttg ctggcgtagt tgcagacgcc gccccaccag ttccccttgc tgacgtaatg
     1561 aacgggagat ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta
     1621 acgcttgcag caacgcatta atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct
     1681 gccacatctt ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc
     1741 tggcttcgct caactggcgc gccatattca tggtaggaat caatgcctga gtattgtctt
     1801 tttccacctg gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta
     1861 acagcgccat cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg
     1921 gtgcacttaa ggtgaaaaag gttgagtcgc aaagtggaat gcatctagca taaagcctta
     1981 ttattgatga ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttgt
     2041 cggcattttc ggctgataac ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag
     2101 cctctatgcc gcttaaagcc aaacgcgtat ggaaactgtg cgcgctttat cccagcctga
     2161 aagattcata ctggttatcg ttgaactatg gtatgcagga ggctgctcgc cgctacggtg
     2221 tggatttaaa agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa
     2281 tcgaccagtg taaacagtgg ggcgcagagg ccattttgct cggtagtagc acgacgtcat
     2341 ttcccgacct gcaaaagcag gtagcaaatc tgccggtgat cgaactggta aatgctcttg
     2401 atgatcccca ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg
     2461 ggcgatattt ggtgcaatgg gcgcacggta acccactgaa cgtgctgctg atgcccggac
     2521 ccgataacgc cgggggcagt aaggagatgg tcgagggttt tcgcgcagcc attgccggaa
     2581 gcccggtgcg tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgcaacc
     2641 tgttgcagga gatgctggaa tgccacccag aaatcgacgt cgttgccgga acggccattg
     2701 cggcagaggc ggcaatgggt gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt
     2761 tttatctttc acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca
     2821 gcgatcaaat ggtctggcag ggggaactgg cagttgagca ggccatcagg caattacagg
     2881 ggcaatcggt gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg
     2941 accgagaaca tatccgccgc tcgctgtcac cggggggatt tcgtccggtc tatttttatc
     3001 agcacacatc agcggctaag aaataacctt caccatgttg cgtcaccagt aaatccgcgc
     3061 tgagtttatg acgtaaacga cgaattaaca catcgacggt gcgtaggtca gggttttcca
     3121 cccgacgcgc agaaagcata cgcagcagac gctcacggct gagaatttcg cccggattag
     3181 tcacaaatgc caccaacatt tcatactctg cgcgggtcag tttaatcggc tcgccatccc
     3241 gctccagcgt atggcgcgac acattcaggc aataacccgc aaagcgatag cagtagtcct
     3301 gagtgtgcgg ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt ttcacccgta
     3361 ctaccagttc gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc
     3421 caacaatacg gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc
     3481 gttctcgcag ggcgcgggtt aacatcaggc cattttcatc gggtaagttg atatccagca
     3541 gaattaaatc taccggctga ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa
     3601 cggaaacggt atacccctcc tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct
     3661 catcttcaac aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatc
     3721 tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc cgctgttcat atttgctcat
     3781 taagatcgct tcactaaacc ataattctac aggggttatt atgcggaaac tctggaacgc
     3841 gctacgccga cccagtgctc gttggtcggt actggcgctg gtcgcaattg ggattgtgat
     3901 tggcattgcg ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt
     3961 ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt
     4021 ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc
     4081 aggcatggtg aagcgcaaac tggaagcgag caacgacatt taccagacct ttattgccca
     4141 ctccattgat acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg
     4201 ggcgcgaatg aaagaaaaca actcggcaac ctgtcgctcc tgccataact acgatgcgat
     4261 ggatcatgcc aggcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa
     4321 tcaatcctgc atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg
     4381 cttccgtaag cagttcgatg agctgcgcgc cagtgctaat gacagtggtg acacgctgta
     4441 ctctagtgat attaagccaa tttatgcggc gaaaggcgat aaagaagcct ctggttctct
     4501 gctgcctgct tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat
     4561 taccggctgg acggaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg
     4621 catctttgtt gcctcgattc gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac
     4681 caccgttgcc gacaccaata ccgagtggag caagttgcag gccaccgcgt ggatgaagaa
     4741 aggcgacatg gtgaacgata tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg
     4801 cacctgtaac cagtgccacg gcgcaccgga aatcgcccac tttgacgcta acggttggat
     4861 cggcacgctc aacggcatga ttggctttac cagtctcgat aaacgtgaag aacgcacttt
     4921 gttgaaatat ctgcaaatga atgcgtctga caccgcaggt aaggctcacg gcgataagaa
     4981 ggaagaaaaa taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca
     5041 caactcggcg gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt
     5101 gcaactgcgg cgcaagcggc gactgaggct gtcatctcga aagagggcat tcttaccggg
     5161 tcgcactggg gggctatccg cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg
     5221 ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat tgccggatca cgtacacaac
     5281 gcggcacgta ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg ccatctgagc
     5341 gacacctccc agcgcggtga taaccgtttt gtgcgcgtga gctgggatga agccctcgac
     5401 atgttctatg aagaactgga acgcgtacag aaaactcacg ggccgagtgc cttgctgacc
     5461 gccagtggtt ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct
     5521 attgccttgc atggtaatag cgttggtacg ggcggagatt actctaccgg tgctgcgcag
     5581 gtgatcctgc cgcgcgtagt cggttcaatg gaagtgtatg aacagcaaac ctcctggccg
     5641 ctggtattgc agaacagcaa aaccattgtg ctgtggggct ccgatttgct gaaaaaccag
     5701 caagcgaact ggtggtgccc ggatcacgat gtttatgaat attacgagca gctaaaagcg
     5761 aaagtcgccg ccggtgaagt tgaggtcatc agcatcgatc cggttgtcac atccacccat
     5821 gagtatctgg ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgacgtgccg
     5881 ctgcaactgg cgctggcaca tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc
     5941 gctaactact gtgtgggttt tgagcagttc ctgccgtatc tgctgggtga gaaagacggt
     6001 cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt
     6061 gggctggcgc ggcagatggc ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag
     6121 cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg
     6181 caaattggcc tgccagatgg tggttttggc tggcactaca acggcgcagg cacgccgggg
     6241 cgtaaaggcg ttattctgag tggtttctcc ggctctacgt cgattccgcc tgttcacgac
     6301 aacagtgact acaaaggcta cagcagcact attccgattg cccgttttat cgatgcgatc
     6361 ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg taaaactgcc gccgctgaaa
     6421 atgtgtattt ttgccggaac taacccattc catcgccatc agcagatcaa ccgcattatt
     6481 gaaggctggc gcaagctgga aacggttatc gccatagata accagtggac ctcaacctgc
     6541 cgctttgccg atatcgtgct gcctgcgacc acgcagtttg agcgtaacga tctcgaccag
     6601 tacggcaacc actccaaccg tggcattatc gccatgaaac aggtggtgcc gccgcagttc
     6661 gaggcgcgca acgactttga tattttccgc gagctgtgcc gtcgctttaa tcgtgaagaa
     6721 gcctttaccg aagggctgga cgaaatgggc tggctgaaac gcatctggca ggaaggtgta
     6781 caacaaggca aaggacgcgg cgttcatctg ccagcgtttg atgacttctg gaataacaaa
     6841 gagtacgtcg agtttgacca tccgcagatg tttgttcgcc accaggcatt ccgcgaagat
     6901 ccggatctcg aaccgctggg cacgccgagt ggcctgattg agatctactc gaaaaccatc
     6961 gccgatatga actacgacga ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc
     7021 tcccacggtg ggcccggctc gcagaagtat ccgttgcatc tgcaatctgt gcatccggat
     7081 ttccgacttc actcgcagtt atgtgagtcg gaaacgctgc gtcagcaata tacggtagcg
     7141 ggtaaagagc cagtgttcat taacccgcag gatgccagcg cgcgcggtat tcgtaacggt
     7201 gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg caggagcagt agtttctgac
     7261 cgctatgcac ccggcgtggc gcgaattcac gaaggggcat ggtacgatcc agataaaggc
     7321 ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg tgctgaccat cgacatcggt
     7381 acttcgcagc tggcgcaggc gaccagtgcg cacactacgc tggtggaaat tgagaagtac
     7441 aatggaacag tggagcaggt gacggcgttt aacggccccg tggagatggt ggcgcagtgc
     7501 gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct gacagcacaa cagattgcct
     7561 gtgtttacgc ctggctggcg cagttgttct cccgtgaact ggacgatgaa caactgacgc
     7621 aaatcgccag tgcgcagatg gctgaatggt tttcgttgct gaaaagcgaa ccgccgctca
     7681 ctgcggcggt gaatgatctg gaaaacagta ttgccacgct gacagttcgt gacgatgccc
     7741 gtctggaact ggccgctgac ttttgcggcc tgtttctgat gaccgacaaa caagtggcgc
     7801 tgccgtatgc atcggcctac aaacaggacg agcaagagat taaacgcttg ttagttgagg
     7861 cagggatgga aaccagtggc aatttcaacg aaccggcaga tcatctggcg atctatctcg
     7921 agttgttgag tcatctgcat ttttcgctgg gagaggggac cgttcctgcg cgaagaatcg
     7981 acagtttgcg gcaaaaaaca ctgacggcgc tgcggcaatg gttaccagag tttgctgcgc
     8041 tttgtcgaca gtatgacagc tttggttttt acgcggcact aagccagtta ttgctggtgt
     8101 tagtggagtg cgaccaccaa aacagataac gtcgtttatg cgcctgaaaa gacgcgttta
     8161 gcgtcgcatc aggcattatg gcgcagttgc cggatgcgtc atcacatccg gcaaacacac
     8221 atcacggatg agctacaaac cgggaaagcc gctggcgtag caggcggttt tcctgcttca
     8281 ggtgcgcaat atcatccatt aacgtcagcg ccaccgcgat ccccggccag tccagagcca
     8341 gttcatgacg caggcgtacc gcgcgttgca ccacaatggc ggcatggtcg tcaaataccc
     8401 aggttgtttc ctggatctca cgcggttcaa ccacccccaa accgacaatt tcattcaact
     8461 cctcttcaga gatgccggta tgcaggcaaa attcggtaat agtaaaagtc accgtaacat
     8521 tagccattat gctttccccc aatctttacg tggatcaaaa gacgactggg cgtctgccag
     8581 ttgctgccac agcgcggcag tgttttcatc cggtttcggc ggcatcacga ttttcagtac
     8641 cgcatacaga tcgccggtct gttttttgct caccagacct ttgcctttaa cgcgcaatcg
     8701 ttgcccggcc tggctgcttg gcgggatagt cagcaaaatg ctttctttca gtgttggaac
     8761 ggtgacttta gtacccagcg ccgcttccca cgggctaacc ggcaccacaa tttccagatc
     8821 ctggccgaca atatcgaaca gcggatgtgg cgcaatatga atcaccagcc acaaatcgcc
     8881 atttggaccg ccgttttcgc ccggcgtccc ctggcctttc aggcggatgc gttgaccatt
     8941 gccgacgcct gccgggatct tcacattcag cgttttcggg atttcctgtt cgatcatgcc
     9001 aaaggcgtta taaaccggca ggttatagct gatggtacgc ttatgctcag taagcgtttc
     9061 ttcgaggaat accgccactt cgatttcaat atcgtggccg cgtgcggcgg ggcgttgacg
     9121 gctctggcgg gcatgctgac cgaaaattga cgagaagata tcgtcaaaat cttcggcatt
     9181 aaaactctga ccgtcgccat gatggaactg acggttaaat tgcggatcgt tgcgatgttg
     9241 ccacatctga tcatactcag cgcgacgttg ttcatcactt aacacttccc aggcttcagc
     9301 gacctctttg aagcgggctt cggcatccgg ttctttgctg acatcaggat ggtatttacg
     9361 ggccagtcga cgataggcgg tcttgattgt cttgagatcg tccgtcggtt tcacgcccat
     9421 gatggcgtaa taatccttta attccatagc gttatctcgc gtaaatcaac acaaattgaa
     9481 ggaacccctg taaggtaact cctataagtg tagggtaatc ctcaaaattt catatgccaa
     9541 cacagaatat gttattgaaa tcatcgcgga gaggaggtcg ccatcaagat gggttgctga
     9601 acatatttta aacaggtgaa aaagggtgag cgatttttga tagttgaacc aggcacttta
     9661 agtttaacta gggcgtcatt atttattaaa ttttatagac gctatatatg ggtagtaata
     9721 tacatggaat tagttgcact gcaaataatt atttgaaaca ggcctggaac gatataaaaa
     9781 atgagtacga aaaaaatcaa acatattcaa tcacgctttt tgaaaacaca ctggtgtgtt
     9841 ttatgcggtt gtacaatgaa ctcagacgta aagtaaatga agaggatact ccatgtctgg
     9901 aatgtgaatc actagaaaaa gaatttgagg aaatgcagaa tgataatgat ctatcattat
     9961 ttatgagaac attgcgtact aatgatacac aaatttattc aggggtttca ggaggtatta
    10021 catatactat acaatatgtt caagatgttg atattgttag agtgtccttg ccgggcagag
    10081 gttcagagtc tatcacagat tttaaaggtt attattggta tggctttatg gaatatattg
    10141 aaaacattaa tgcgtgtgat gatgtttttt ctgagtattg tttagatgat gaaaatatga
    10201 gtatccagcc agaacagata aatatgccgg gaatatctga tttagatact ggtattgatt
    10261 tgtctggcat atcttttatt cagagtgaaa ttaataaaac atatggatta aaatacgctc
    10321 cagtagatgg cgatggatat tgtctgttaa gagccatact ggttttaaaa gaacatgagt
    10381 attcatgggc gctgggcagc cataagacgc aaaagcaagt atacgaagag tttattaaaa
    10441 ttgttgataa acaaacgatc gaggctcttg ttgatacggc attctataat ctcagggaag
    10501 atgtaaagac attatttggc gttgatctgc aatctgacaa caaaattcag ggtgtgggta
    10561 gttttatgtc atggagcttt ctgtttttta aaaaacaatt cattgatagt tgcttgaatg
    10621 acaaaaaatg catactgcat ttacccgagt ttatatttaa tgataacaag aatttgcttg
    10681 ctttagatac cgacacgtcg gataggatta aagcggtgaa gaattttctt gctgttcttt
    10741 cagatagcat ttgctcatta tttattgtta atagtaatgt ggcatcaatc tccttgggaa
    10801 atgaatcctt ttcaacagat gaagatcttg agtatgttta tttaataaac actggcaatc
    10861 attatgatgt ttacctccct cctgaacttt tttctcaggc ttataagtta aaaaataagg
    10921 aaatgaatgc gcaacttgac tatttaaatc gttatgcaac ttaatggcaa aggcatatgc
    10981 taaaaaccat tgttattagt ctcacacttt tttattggta aatattgtct ctgtattggt
    11041 aacgccgcag atattctgtt tagccacagg tgcaattatc agcggcgtac gcgaggcagg
    11101 ggctaatcag gcatagtttg cgtcaaacct tgcctgtttt tgaagatgta tatagaaaaa
    11161 caggcgttca acaagccatt ttgcgaacct gttcccggaa aaaagtcata tttctgtcac
    11221 actctttaat gattgataac aaaagaggtg ccaggaatga acaaaacgct aatcgccgca
    11281 actgtggcag ggatagtttt actcgcttca aatgctcagg cacaaaccgt accggaaggc
    11341 tatcagctac agcaagtgct catgatgagc cgccataact tacgtgcgcc gctggcgaac
    11401 aatggcagtg tgctggagca gtcgacgccg aataaatggc ccgaatggga cgtccccggt
    11461 gggcaactca ccaccaaagg tggcgtgctc gaagtgtata tgggccatta catgcgtgaa
    11521 tggctggcag agcaggggat agtgaaatcg ggggaatgcc cgccgccgga caccgtttat
    11581 gcctatgcca atagtctgca acgtaccgtc gctaccgccc agttctttat tactggtgca
    11641 ttccctgggt gtgatattcc tgtgcatcac caggaaaaaa tgggcactat ggacccaacc
    11701 tttaacccga tgatcaccga tgattccgcc gcattcagtg aacaggcggt ggcggcaatg
    11761 gagaaagagc tcagcaaact ccagcttacc gacagctacc agctactgga aaaaatcgtt
    11821 aactataaag attcccctgc ctgtaaagag aaacaacagt gttcgctggt ggatggcaaa
    11881 aataccttta gcgccaagta tcaacaagaa ccaggtgttt ccgggccgct gaaagtcggc
    11941 aactcgctgg tagatgcgtt tactttgcaa tactacgaag gttttccgat ggatcaggtg
    12001 gcctggggag aaatcaaatc tgaccagcag tggaaggtat tgtcgaagct gaaaaacggt
    12061 taccaggaca gcctgtttac ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc
    12121 agttatatcg acaaagccct ggtcaccgat cgcgccagtg ctccgaaaat cacggtgtta
    12181 gttgggcacg actccaacat agcctctctg ttaacggcgc tggatttcaa accgtatcag
    12241 ttgcatgacc agaacgaacg cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat
    12301 gacagcaaag ccaatcgcga tttgatgaaa attgaatatg tgtaccagag tgcggaacag
    12361 ttacgtaatg ccgatgcgtt aaccctgcag gcacctgcgc agcgcgtgac gctggaatta
    12421 agcggttgcc cgatagatgc caacggtttc tgcccgatgg ataagtttga tagcgtgttg
    12481 aatgaggcgg tgaaataaca gaaaactccc ccgcgagaag cgggggagtc gctggttaaa
    12541 cgtttttacg ttcgatggtc tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa
    12601 aggctttgac taacacttca tcactacctt cttcccaaat cttttctgcc attttttcgt
    12661 cgtacccggc gacttcgaaa atggcctcgg ctatttccgg cgacgtattg cgcagagatg
    12721 cccattcacc gacgtgatga gctttcgctt cttgagttgg catgcgtatc ctcctgttga
    12781 agattagccg ttaagtttaa ctgccagacc tgcgacatat tccccttgat aacgagcaat
    12841 agacagttct tcctggcttg gctggcgtga gccgtcaccg cctgcgatgg tggttgcgcc
    12901 gtacggcgta ccgccgcgaa cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat
    12961 ggggacaatc accatgccgt gatgcgcaag ggtcgtccag gtggatgtga tggtttgttc
    13021 ctgaccgccg ccagtaccgg tggaactaaa gacgctcgcc agttttccgt atagtgcgcc
    13081 ggaagcccac aggccgcccg tctggtcgag gaaggtacgc atttgaccgg acatgttgcc
    13141 aaagcgggta ggtgtaccaa aaataatggc gtcgtaatcg gccagttctt gcggggttgc
    13201 aaccggtgca gtttgcgttt taccgcctgc tttttcaaat aattgcggcg gcatggtttc
    13261 cggtacacgc ttaacgacaa cttctgcgcc atccactttg cttgcaccct cagcgactgc
    13321 gcgtgccatc gtttcaatat gtccgtacat ggaataataa agcaccagaa ctttagccat
    13381 ttctaaccac tcctcgtgtt atctctattc cgtagcgatt cgctaccact tatttaaaga
    13441 taagacgtcc ttttcagagt gcaaatttca caaccactta tttgatttat aacaactttc
    13501 acaagcacgt aattttgtcg caaaatgaca catttttatc tcatcgcgtt tttttaatta
    13561 taagagcggc ttatggataa ttattggaga tgatatctat tctcgctaag aagctgttgc
    13621 aggatattac caaacgcggg tctgcccgcg tcagttcact aagcttagtc ccacgtagcg
    13681 aaaatatggc agccgccata cgccgcgtta attctatgca atatgatgtc tatacccaga
    13741 cggaggtcag taatggcaaa ccatcgaggc ggttccggca attttgcaga agaccgcgaa
    13801 agagcatcag aagcaggtaa aaaaggtgga cagcacagcg ggggtaattt caagaatgat
    13861 ccgcagcgcg catctgaagc aggtaaaaaa ggtggcaaga gcagtcacgg caaaagcgac
    13921 aactagccgg gctaatcaat gacgaatgca tttttttctt aagctcgtca aaaagtcatc
    13981 accgccggta acccggcggt tttctgttta tggctcctga tgaacaactt ctggcggtgg
    14041 aacgtcaacc aaccttctgc ttaataacgc attgagtaaa atcgcgccaa aggttgctgt
    14101 accgatccct cccaacgtaa aaccgccaag cgtgagagca aaatcacccg cgcccagcac
    14161 gaaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac ggttttgtac
    14221 ccatatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg caccaccaat
    14281 caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc ccaacagcat
    14341 agcgatgacg gcagctgcaa caaacaccag cgtcgagtag acttttgtca cggccatcac
    14401 accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg aaagcatcgt
    14461 tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc gtccggtcat
    14521 cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg ccacgggcgc
    14581 aatcaacatc atcgcctggc cattaaaacc aggagtggaa aaatgcggca gaccgaacca
    14641 ggcagcatag ctgacgagag taaaatcgac ggcttttccc agccctaaaa cgttggtcat
    14701 cacgccatac agcagacagg cgacaattaa tcctacgaga atcaataacc gctggatcat
    14761 gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg ccatccagct
    14821 atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca ggccaatcgc
    14881 catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc gtgtaccaat
    14941 tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa tcccgcccag
    15001 cgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg caatcaccac
    15061 gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg taataaagaa
    15121 aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca gccccatcag
    15181 aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc ccattactgc
    15241 cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg tagaggtcga
    15301 ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta aggaggttaa
    15361 caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc gtagggtgtg
    15421 gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc gacgctcacc
    15481 acctggctga tacggcagtc aaacgaaacc agagcctctt ccagttgcgg gcatccggtc
    15541 acactcgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt gccgccaaaa
    15601 agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt tcgattttca
    15661 ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt gggcggcgtg
    15721 tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggctgg accgtccgtg
    15781 gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt ttgttgatca
    15841 actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg acagacgtgg
    15901 caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca gattgctctt
    15961 cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac ccgacatcgg
    16021 gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct gcatggaact
    16081 gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac tgcgggcatc
    16141 accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg cgacgatagc
    16201 ggtgacgggc gcggtcaggg ttttttgcag attgccgctg gaaagcgccg ggcgcagacg
    16261 ttcttttcct tctgcagtgc ggataaacac gatccgtgcc ggagaacagt tagctgatgt
    16321 cggcccccat ttcatcaggg cataaagctc ctgtaacgtc tcatcgctga cgggtgtctc
    16381 ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac ctgggctaac
    16441 ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca ggctggcaag
    16501 cccgttgagt aacagagcat taaacgtttc gggatcggtc acgttgcagg cgtgtccgcc
    16561 atagggcatc accattttct ggctatcggg cagggcggca tgaagttcac tggaacatgc
    16621 tgttggcacc agcagatcat cactggcgca gatgatttgc accgggcagc ggatgcgatc
    16681 cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgtagta aattattttt
    16741 gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctcc aggcgaggtg cgcgggccgc
    16801 catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgctt gcgcgccgcc
    16861 gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt ttattcgtag
    16921 ccagccgtta acgcaacgct gaccagcata gttaccgacg cgggataatc cagcgccagc
    16981 tgcatcccca ccagcgcacc gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg
    17041 gctaccagcg cctgatgcag ttccgctgcc atctgggcga tactgtaatc ttctgccagt
    17101 gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc
    17161 agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc
    17221 actacgggtg catcagcata agggggaggt gagagtgaaa gtttcatcgc ggcctcactt
    17281 ggcgatatgc gcaattgtgg cgatttccac cagtgcgtca ggttttacca gtccgcactg
    17341 aatgcagaat cgcgccggtt tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc
    17401 ggcgtaattt ttccagtcgg taataaagat gctgttgaag gtcacatccg ccatcgtgcc
    17461 acccgccgtc tcgatcacct tgcggatagt ttccagaacg tggcgggttt gcgcctttgg
    17521 gtcatcggca aacagcacgt tattatgttg atcaaaagcc agcgtaccgg agacatacac
    17581 cacgccatca gccagcgtgc cgggaacgaa gggggccagc ggtgcgctgc tgccagcggg
    17641 aataattacg gattttggca tcgttaaact ccttaagcga tacgagcaaa ggacgtggga
    17701 gaaagcgcgt cgcagaacgt ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg
    17761 aacaacgcgg ctttctgtgc aaattccggc cccgcctggt gtgttgcgtc ttccagcacc
    17821 acgccgaaat actccagaaa aaagccatcg cgtagcgtcg attcgacgca gacgttggta
    17881 gcgataccgg tgaaaaccag atggcgtatt ccacggctgc gcaaaatgct gtccagcggc
    17941 gtattgaaga aaccgctgta gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc
    18001 agttcatcca ccagttgata atcccaggag cctttcgcca gcaatttccc ctgcagctgc
    18061 ggctgcttac gcatggtttt cagggcgttc gatttatgaa aattcggtga gccggggccg
    18121 ccagcctcga catactgttc atcccagcca ttttgaaacc agatgatcaa catccctgcc
    18181 gctcgcgctg cggtcacagc ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca
    18241 tcaaacccgg cgagatctaa gtagccgcct ggcgtggcat aagcgttttg catatccacc
    18301 acgatctgcg cactttgctg cggatcgaag gtaatggctt ccggtcgagc ggttaaggtt
    18361 gtcatcatgc cacctcctga gtcagcgcag agagatgggc gcggcactgc atcagtggtt
    18421 gaatgcgctc gccgaaggtt tcgattcccg acagaaaatc atcgaaggtc aacagcacgc
    18481 cttctgcacc aggcacgctt gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac
    18541 cgactaacgt ccccatattg atgtttaccg ccgaagtggg atcggccatc tgacgaacgt
    18601 tggtgtcagt accggagcgg gtatctttct gactttgttc ggttagccag cttaacgcct
    18661 cttcatccgc gcccgctttg tagtgttccc atttggcgcg agtggcatcg tcggtttcat
    18721 cggcaatcac cataaacaac acataagagc caacgtcgcg tccggtttgc tctgcggcct
    18781 gtttcatccg cgcagcggtc ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac
    18841 agaagttgaa atcggcatac tgggcggaga acgccatgcc agcgtcgctt tgcccggcgc
    18901 agatcacttt catggggaca ctcggttgcg gactgacgcg acaatcattc atggtgaaaa
    18961 aatcgccttt aaaatcgctt ttccccgtgc cccacaggtc gcgcagcacc tgaacatatt
    19021 cggtgagata gtcgtaacga cgggagaaat agtcatcgcc aggccagata cccatctgct
    19081 catactcggg cttttgccag ccagtcacga ggttgacgcc aaaacgcccg ccagaggtgg
    19141 agtcgatggt tgcggccata cgggcgatga ttgccggagg taacgttaag gtggcagcag
    19201 tggcgtaaat ctgaatgcgc gaggtcacgg ctgccagacc cgccatcaag gtgaacgact
    19261 caaggttgtg atcccagaac tcagttttgc cgccaaagcc acgcagtttg atcatcgaca
    19321 gggcgaaatc gaaatggtaa tgctccgctt tttgcacaat ggctttattc agttcaaagg
    19381 tcggcatgta ctgcggcgcg tgggtcgaaa tgagccagcc gttgttgcca ataggtacga
    19441 atacgccaat tttcatcatc aacctctctt cgtctcgtaa agtgaaagtc agacggggcg
    19501 ctgcatcctg catatccttt tcagccgcgt attggcttgt ttgcaaagcg gatgccagtt
    19561 tttaaaaagt gaatgttatt aatgtgttag cattgtgtta tctaaaatat ctggtaaaaa
    19621 gtggactaaa cggtcaaaac agttgcacat aaaacatgca tccgtgcgcg atgagagtgc
    19681 agaaggtcga ggccgggcgg gggttttgct atcctgttac caatctacaa gaggggagag
    19741 cgcatgacgc aaggcgcagt gaaaacaacg ggtaaacgtt cgcgcgcagt aagcgcgaag
    19801 aaaaaagcga ttcttagcgc agcactggac actttttcac aattcggttt tcacggcaca
    19861 aggctggagc agatcgcaga gttggcgggt gtttcaaaaa ccaatctgct gtattacttc
    19921 ccgtcaaaag aggcgctgta tattgccgtg ctgcggcaga ttctcgatat ctggctggca
    19981 ccgttaaaag cgtttcgtga agatttcgcc ccgctggcgg cgataaaaga gtacatccgt
    20041 ctgaagctgg aagtctcacg cgattatccg caggcttcgc gcctgttctg tatggagatg
    20101 ctggcaggcg cgccgttgtt aatggatgaa ctgacgggcg atttgaaggc attaattgat
    20161 gagaaatcgg cgctgattgc cggttgggtc aaaagcggca aactcgcgcc gattgatccg
    20221 cagcatttga tttttatgat ttgggcttcc actcaacatt acgccgattt cgcccctcag
    20281 gtggaggcgg tgacaggcgc gacgttgcgc gatgaggtat ttttcaatca aacggttgaa
    20341 aacgtgcagc ggattattat tgaggggatt cgaccacgtt aaagatgccg gaggaggttg
    20401 taacatcctc cggctacctg tttaaccgat cgtcattaaa ctggcgttac cgccagcggc
    20461 agcggtattc acactcagcg aacgttcatc gataggaatt aaaaccccaa aaagattaaa
    20521 aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga acggaaccga
    20581 cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt tgctccgttt
    20641 ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg catccg
//
LOCUS       DADKXM010000062        20575 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.062, whole genome shotgun sequence.
ACCESSION   DADKXM010000062 DADKXM010000000
VERSION     DADKXM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.062"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JQR05_002263"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JQR05_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY5324429.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JQR05_002264"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JQR05_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY5324430.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JQR05_002265"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JQR05_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY5324431.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAERQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQR05_002266"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQR05_002266"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY5324432.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQR05_002267"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQR05_002267"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY5324433.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGREPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JQR05_002268"
     CDS             4449..4814
                     /locus_tag="JQR05_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY5324434.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JQR05_002269"
     CDS             4964..5482
                     /locus_tag="JQR05_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY5324435.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JQR05_002270"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JQR05_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JQR05_002271"
     CDS             6713..7195
                     /locus_tag="JQR05_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY5324436.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQR05_002272"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQR05_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY5324437.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQR05_002273"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQR05_002273"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY5324438.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQR05_002274"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQR05_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY5324439.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQR05_002275"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQR05_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY5324440.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQR05_002276"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQR05_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY5324441.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JQR05_002277"
     CDS             10864..11730
                     /locus_tag="JQR05_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY5324442.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JQR05_002278"
     CDS             11751..13037
                     /locus_tag="JQR05_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY5324443.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQR05_002279"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQR05_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY5324444.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JQR05_002280"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JQR05_002280"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY5324445.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JQR05_002281"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JQR05_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY5324446.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JQR05_002282"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JQR05_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY5324447.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JQR05_002283"
     CDS             complement(16985..17275)
                     /locus_tag="JQR05_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY5324448.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQR05_002284"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQR05_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY5324449.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JQR05_002285"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JQR05_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY5324450.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGSNITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JQR05_002286"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JQR05_002286"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JQR05_002287"
     CDS             19137..20378
                     /locus_tag="JQR05_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5324451.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5311 a         5363 c         4833 g         5068 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcactaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgctaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacaggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gagcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggctcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaaccta gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccaa ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgcta
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttatttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggctctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagaaacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttagt gtatgatggt gattttaagg tgctt
//
LOCUS       DADKXM010000063        20440 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.063, whole genome shotgun sequence.
ACCESSION   DADKXM010000063 DADKXM010000000
VERSION     DADKXM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.063"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..213)
                     /locus_tag="JQR05_002288"
     CDS             complement(<1..213)
                     /locus_tag="JQR05_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HAY5324452.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKE"
     gene            complement(221..931)
                     /locus_tag="JQR05_002289"
     CDS             complement(221..931)
                     /locus_tag="JQR05_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY5324453.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1147..2970)
                     /gene="typA"
                     /locus_tag="JQR05_002290"
     CDS             complement(1147..2970)
                     /gene="typA"
                     /locus_tag="JQR05_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY5324454.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3343..4752
                     /gene="glnA"
                     /locus_tag="JQR05_002291"
     CDS             3343..4752
                     /gene="glnA"
                     /locus_tag="JQR05_002291"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY5324455.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4925..5974
                     /gene="glnL"
                     /locus_tag="JQR05_002292"
     CDS             4925..5974
                     /gene="glnL"
                     /locus_tag="JQR05_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY5324456.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5986..7395
                     /gene="glnG"
                     /locus_tag="JQR05_002293"
     CDS             5986..7395
                     /gene="glnG"
                     /locus_tag="JQR05_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY5324457.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7507..7617
                     /gene="yshB"
                     /locus_tag="JQR05_002294"
     CDS             7507..7617
                     /gene="yshB"
                     /locus_tag="JQR05_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY5324458.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7846..9219)
                     /gene="hemN"
                     /locus_tag="JQR05_002295"
     CDS             complement(7846..9219)
                     /gene="hemN"
                     /locus_tag="JQR05_002295"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY5324459.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9408..9917)
                     /gene="yihI"
                     /locus_tag="JQR05_002296"
     CDS             complement(9408..9917)
                     /gene="yihI"
                     /locus_tag="JQR05_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY5324460.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDVLLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10499..11131
                     /locus_tag="JQR05_002297"
     CDS             10499..11131
                     /locus_tag="JQR05_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY5324461.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11513..14299)
                     /gene="polA"
                     /locus_tag="JQR05_002298"
     CDS             complement(11513..14299)
                     /gene="polA"
                     /locus_tag="JQR05_002298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY5324462.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14301..14543
                     /locus_tag="JQR05_002299"
     CDS             14301..14543
                     /locus_tag="JQR05_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324463.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            14663..15595
                     /locus_tag="JQR05_002300"
     CDS             14663..15595
                     /locus_tag="JQR05_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY5324464.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15636..17066)
                     /locus_tag="JQR05_002301"
     CDS             complement(15636..17066)
                     /locus_tag="JQR05_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY5324465.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNDAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(17221..17847)
                     /gene="dsbA"
                     /locus_tag="JQR05_002302"
     CDS             complement(17221..17847)
                     /gene="dsbA"
                     /locus_tag="JQR05_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY5324466.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(17864..18850)
                     /gene="srkA"
                     /locus_tag="JQR05_002303"
     CDS             complement(17864..18850)
                     /gene="srkA"
                     /locus_tag="JQR05_002303"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY5324467.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(18927..19196)
                     /locus_tag="JQR05_002304"
     CDS             complement(18927..19196)
                     /locus_tag="JQR05_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY5324468.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19266..19850
                     /gene="mobA"
                     /locus_tag="JQR05_002305"
     CDS             19266..19850
                     /gene="mobA"
                     /locus_tag="JQR05_002305"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY5324469.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            19832..20359
                     /gene="mobB"
                     /locus_tag="JQR05_002306"
     CDS             19832..20359
                     /gene="mobB"
                     /locus_tag="JQR05_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY5324470.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         4969 a         5164 c         4950 g         5357 t
ORIGIN      
        1 ttctttatcg tccatattac caagagaaaa acgctcgcca tccagaacat ggattttgac
       61 gccacgtaga ttattttcac ttgcgatatc aagtaaatca gcaggcaaca ctctttccag
      121 ccgcatattt aaatgaaagg cataagcatg taaataaaga ggaagagtgt cgacacgttg
      181 tagaatcttt cttgcgttat ttattgtcac cataaaacac ctacagatta atgatgaggt
      241 ctacggcctc aaatcgccaa tattcaatat ctatctggac aatattgcct tgctggtcgg
      301 cgcgatgctt ttcaattaag atcgctggca tacccgcaga accgccaaga tacttgctga
      361 tatcgcccgg cattctgacg ggtttgaaaa tcaatttttt tactaccgtc tctttttggt
      421 agcacttttc ccagactgca gaaaatgagt ggttttcaag ttgttcaata aaacccggag
      481 caacttcagg attaatatat gtttcgtgat agaaaacttt atgtccttcc agcgcccccc
      541 agccggtgat gcgatagagt tcagttgatg gcgtcactgc aatcagtggc gcgagcgtct
      601 cgggaatatc gctggtacgg tttttctcgg taaaccccca agaaggctct cgcccttgct
      661 caattgcggc ccgctgaaag ctcgccgaca gctccggact gtaattaaat cgcggctggg
      721 tcacgaacca ccccttacga tccttacgga agattttgga ttctgcctcg aggttcaaca
      781 acgcctggcg cagcgtcata cgtttaatgc ccagcagttc tcccagctct ctttctgaag
      841 ggagttttcc acccggcgtg gtgatgccat ctttcaacca ccgatccagt ttctcttttg
      901 cattttctac ggtggattga ttctccgcca ttgtttaatc cttttgattt aaaccagatg
      961 aagcatctta cgcttacgcc aaaaaagaaa gcaattaacg gtgtatgact ttgcagataa
     1021 ttgtgacaaa gctctctatt gacgtaatca cagacttata agggagatac tcgcattggt
     1081 gtgcgctcac gacaggtaaa aaaaacctgc catcgctggc aggtttttta tgactaaaaa
     1141 acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga cgatcgtttt ccgtcaggtg
     1201 acgtttacga atacggatag aggtcggagt cacttctacc agttcgtcat catcgatgaa
     1261 ctccagagct tgttccagag tcatgcggat aggcggaacc agaacaacag cttcgtcagt
     1321 accggaagca cgcatgttgg tcagtttctt accggtcagg cagtttacag tcaggtcgtt
     1381 agagcggcta tgaataccga taatctgacc ttcgtaaact tctgcaccgt gaccgaggaa
     1441 cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg acagctttac cctgaccgtt
     1501 agagatcagt acaccgttct gacgctgacc cacttcgccc ggacgtacgt cgtcgtagtg
     1561 gctgaaggtg gagtacagca gaccagtacc ggaagtcatg gtcatgaact cagaacggaa
     1621 gccaatcaga ccacggcttg ggatcacgta gtcgagacgt acgcggcctt taccgtctgg
     1681 attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc tgcatcacag aaccctgatg
     1741 ctgttcttca acgtccagcg tcacgttttc atacggctct tgtttacgac cgtcgatttc
     1801 acggaagatt actttcggac gggataccgc cagttcgaaa ccttcacgac gcatgttttc
     1861 gataagaaca gagaggtgca gttcaccacg accagaaacg cggaacgcat cggcgtcttc
     1921 ggtttcttct acgcgcagcg caacgttgtg taccagttct ttgttcagac gatcgaggat
     1981 ctgacgagac gttacgaact taccttcttt accgcagaac ggcgaggtgt taacgcagaa
     2041 gaacatagaa acggtcggct catcaacgga gagtgccggc agcgcttcaa cgttttgcgt
     2101 gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc gtgatcgcca cgatatcgcc
     2161 agcttccgcc agatcggttt cgatacgttc cagaccgagg tggcccagca ctttaccgac
     2221 tttcgcgtta cgggttttgc cttcgctatc gatgatagta acctgctggt tcggcttcac
     2281 tttaccacgc ttgatgcggc caatgccgat aacgccaaca tagctgttgt aatcgagctg
     2341 agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc ggcgcaggaa cgtggtcaac
     2401 aatcgcctga tacagcgggg tcatgtcttc cgccatatct tcgtggtcca gacccgcgat
     2461 accgttcagc gcagaagcgt aaacgatcgg gaagtccagc tgctcgtcgg tcgcgtcgag
     2521 gttaacgaac agatcgaata cctgatccac aacccaatca ggacgcgcgc cagggcggtc
     2581 aactttgttg ataacaacaa tcggcttcag gccgtaagca aacgcttttt tggttacgaa
     2641 gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc agcagcactg agtctaccat
     2701 ggacattaca cgttcaactt caccaccgaa gtcggcgtgc cccggggtat caacgatgtt
     2761 gatacggtaa tcattccatt tgatagcggt gtttttcgcg aggatggtaa tcccacgctc
     2821 tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt tcggcacgag agtcgaacgt
     2881 accggattgt tggagcagct tgtctaccag ggtggtttta ccatggtcta cgtgcgcgat
     2941 gatggcgata ttacgcaatt tttcgatcac aactttgcct caggcattag aaatagcgcg
     3001 ttattgtaca cggattaatc gcactacaaa acaggatcac aaacatcctc cgcaaacaag
     3061 tattgcagag tccctttgtg atcgctttca cggagcataa aaagggttat ccaaaggtca
     3121 ttgcaccaat atggtgctta atgtttccat tgaagcacta tattggtgca acattcacat
     3181 catggtgcag cccttttgca cgatgttaag catgataacg ccttttaggg gcaatttaaa
     3241 agttggcaca gatttcgctt tatctttttt acggcgacac ggccaaaata attgcagatt
     3301 tcgttaccac gacgacaatg accaatccag gagagttaaa gtatgtccgc tgaacacgta
     3361 ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt tgcgcttcac cgataccaaa
     3421 ggtaaagaac aacacgtcac tatccctgct catcaggtga atgctgaatt cttcgaagaa
     3481 ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag gcattaacga atccgacatg
     3541 gtgctgatgc cagacgcatc caccgcagtg attgacccgt tcttcgccga ctccaccctg
     3601 attatccgtt gcgacatcct tgaacctggc accctgcaag gctatgaccg tgacccgcgc
     3661 tctattgcga agcgcgccga agattacctg cgttccactg gcattgccga caccgtactg
     3721 ttcgggccag aacctgaatt cttcctgttc gatgacatcc gttttggatc atctatctcc
     3781 ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga actcctccac ccaatacgaa
     3841 ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt acttcccggt tccgccggta
     3901 gactctgctc aggatattcg ttctgaaatg tgtctggtga tggaacagat gggtctggtg
     3961 gttgaagccc atcaccacga agtagcgact gctggtcaga acgaagtggc tacccgcttc
     4021 aataccatga ccaaaaaagc tgacgaaatt cagatctaca aatatgtcgt gcataacgta
     4081 gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac cgatgttcgg tgataacggc
     4141 tccggtatgc actgccacat gtctctgtct aaaaacggcg ttaacctgtt cgcaggcgac
     4201 aaatacgcag gtctgtctga gcaggcgctg tactacattg gcggcgtaat caaacacgct
     4261 aaagcgatta acgccctggc aaacccgacc accaactctt ataagcgtct ggtcccgggc
     4321 tacgaagcac cggtaatgct ggcttactct gcgcgtaacc gttctgcgtc tatccgtatt
     4381 ccggtggttt cttctccgaa agcacgtcgt atcgaagtac gtttcccgga tccggcagct
     4441 aacccgtacc tgtgctttgc tgccctgctg atggccggtc ttgatggtat caagaacaag
     4501 atccatccgg gcgaagccat ggacaaaaac ctgtatgacc tgccgccaga agaagcgaaa
     4561 gagatcccac aggttgcagg ctctctggaa gaagcactga acgaactgga tctggaccgc
     4621 gagttcctga aagccggtgg cgtgttcacg gatgaagcga tcgatgcgta catcgctctg
     4681 cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc cggtagagtt tgagctgtac
     4741 tacagcgtct aatagttgaa gttgtactac ccggcgcaca acgccgggat ttagttgccg
     4801 tggaaacttt cagcccatct ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg
     4861 cgctttttat ccgtaaaaag ctataatgca ctaaaatggt gcaacctgtt caggagactg
     4921 ctttatggca acaggcacgc agcccgatgc tgggcagatc ctcaactcgc tgattaacag
     4981 tattttgtta attgatgaca atctggcgat ccattacgcc aaccccgccg cgcaacaact
     5041 gctcgcccaa agctcccgca aattgtttgg tacgccgtta ccggaactgt tgagctactt
     5101 ctcattaaat atcgagctga tgcaagaaag tctggaggcg gggcaaggct ttaccgataa
     5161 cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt tctgtgacgg cccagcgtat
     5221 gccggacggc atgatcctgc tggagatggc accgatggat aaccagcgcc gcttaagcca
     5281 ggaacaacta caacatgctc aacaagtagc tgcccgtgat ttagtgcgcg gcctggcaca
     5341 tgagattaaa aatccgcttg gcggtttacg tggcgcggcg cagttactca gcaaagcgtt
     5401 acctgaccca tcactactcg aatataccaa agtgattatc gaacaggcgg accggctgcg
     5461 aaatttggtc gaccgtctgt tggggccgca gctgcccggt acgcgcgtta ccgaaagtat
     5521 tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg
     5581 gttgattcgt gattacgatc ccagcctgcc ggaactggcg cacgacccgg atcaaattga
     5641 acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga
     5701 aatcattctg cgtacccgca ccgcgtttca actgacctta cacggcgagc gctaccggct
     5761 ggcggcgcgg attgatgtgg aagataacgg gccgggcatt ccgcctcatt tgcaggatac
     5821 gctgttttac ccgatggtca gcggccgcga aggtggcacc gggcttggct tatccatcgc
     5881 ccgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc caggtcatac
     5941 cgagttctcg gtttacctgc ctatcaggaa ataaaggtga cgtttatgca acgagggata
     6001 gtctgggtag tcgatgacga tagttccatc cgttgggtgc ttgaacgtgc gctcgctggc
     6061 gcgggtttaa cctgtacgac atttgagaac ggcgcggaag tactggaggc gctggcgagc
     6121 aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg gaatggacgg gctggcgctg
     6181 ctcaagcaga ttaaacagcg ccatccaatg cttccggtca tcattatgac cgcacattcc
     6241 gatctggatg ctgccgtcag cgcctatcaa caaggggcgt ttgattatct gcccaaaccg
     6301 tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta tcagtcatta ccaggaacag
     6361 cagcagccgc gtaatattca gcttaacggc ccaacgaccg atatcatcgg cgaagcgcaa
     6421 gccatgcagg acgtgttccg tattatcggt cggctttcgc gttcttctat tagcgtgctg
     6481 attaacggcg aatccggcac cggtaaagaa ctggtcgctc atgccctgca tcgccacagt
     6541 ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag ctatcccaaa agatttgatc
     6601 gaatcagaac tgtttggcca cgagaaaggc gcgtttactg gcgcgaatac cattcgtcag
     6661 gggcgttttg aacaggccga tggcggcacg ttatttctcg acgaaattgg tgatatgccg
     6721 ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg gtcagtttta tcgcgttggc
     6781 ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg ccactcacca gaatcttgaa
     6841 cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc accgcctgaa cgttatccgc
     6901 gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc cccgtctggc gcgccatttt
     6961 ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa
     7021 gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc
     7081 tggctaacgg tgatggccgc cgggcaggaa gtgttgattc aggatttgcc cggcgaactg
     7141 tttgaatcaa cggttgcgga gagtacttcg caaatgcaac cggacagttg ggcgacactt
     7201 ttagcgcagt gggcagacag agcgctgcgt tccggtcatc aaaatctgct ttccgaagcg
     7261 cagccagagc tggagcggac gttactgacg accgcgttgc gacatacgca ggggcataaa
     7321 caggaagcgg cgcggctact cggctggggc cgcaacactc tgacgcgtaa gttaaaagag
     7381 ctggggatgg agtgattcac ggcttgtgtg taaagattga ttattgagcg caaattgctg
     7441 gtattttgcg ctttactgtt ccgataagtt cagtatgatc ttgcccggaa aacggggaga
     7501 gtcattatgc tggaatcaat aattaatctg gtatcgagtg gcgcagttga cagccacaca
     7561 ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga ttgggctgtt tagctgaagc
     7621 tcgttgttta tgccggatgc ggagaaagtc gtaggcctga taagacgcga ccagcgtcgc
     7681 atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc cttatccggc ctacaaaagc
     7741 aataatttca acaggttttg ttgcccagcg catcaggcaa tcttgttctc agcacattga
     7801 tggcgctttc gttttctact ttgtaaacga agcgccattc actctttaaa tcacccgtga
     7861 gaactgctgc atccgcgctt tctggcgcag ataggtatca aagcacatgc aaatgttgcg
     7921 gatcagcaag cgacctttcg ccgtcacctg tattcccttc tcatccacat ccaccagccc
     7981 atcttttgct aacggggcga gcagcttgag atcttccgca aagtaatcag cgaagtgcaa
     8041 atcccactgt ttctcaagag gggcgtaatc cagacggaag ttgcagatga gcgacttaat
     8101 cacatcgcgg cgaatacagt catcacgcgt tagcgcaata ccacgccaca gcgcattgcc
     8161 ttgttcatcc acttgctgat agtactgctt caactctttc tggttctgcg cgtagcagtc
     8221 gccaatcatg ctgatggcgg aaacgcccat ccccagcaga tcggtatcgc cctgagtggt
     8281 gtagccctgg aagttacgat gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc
     8341 aggacgggca aagtgatcca taccgataaa ctgatagccc gattgcgtca ggaaggcgat
     8401 ggtttcctgc aggatatcga gtttttgctg cggactcggc aggtcagcat ctttgatttt
     8461 gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta aaaacgctca gacgatcggg
     8521 gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag
     8581 gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc tcacgcgcat ggttaagcag
     8641 tgcaaagatg aactcttcat cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa
     8701 gtcctgcacg cccatgctca ggcgattaaa gccttcggcg cgtaaatgat cgagtacatc
     8761 cagttcgatt tcccgcggat cgacttcgat cgaaatctcc gcatcggtat tgaaatggaa
     8821 gttttcgcgc agcagcttca tcagacggct gatttgcgct ttattcagat acgtcggcgt
     8881 tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg aacagcggtg cacgatggac
     8941 gatttcttgc tccagcgcgt ccagatactg atcggccttg tgctgctggc gagtaacaat
     9001 cttattgcaa ccgcagaagt aacaaagctt atggcagaac gggatatgta cgtagagaga
     9061 taatggacgc tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc
     9121 tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt tatatttctg
     9181 gatcagggcc agatcccagt cgatttgctg tacagacatg ctcactcctt ccgatggcgt
     9241 ctctggcggc tacggcgtac cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc
     9301 cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata accacaccag ataacatata
     9361 accggaaggg ttatcagcag aacgggaagg cccacggcgg caatccgtta gttgccccgc
     9421 agcagacgca tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa
     9481 gagaggccga gtttctgcat caactcatca atacgatcca gtttagcatc cacccaggat
     9541 tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt ccagcagcac atccagacgc
     9601 tcatccgttt ccagtaattc caactccgcc tgcggtgaaa gcataggttt ctcactcttc
     9661 ggtttgtgct gtttggtgac tttttcagtc actcccaatg gaataggggt tttactgcca
     9721 atacgtggat cttttggtgc gttctggcct ttgctgcctg acgtggtgtt tccgcccgct
     9781 gcgcggctgc ccggcgcatg accacgacgt tttttctgac gcttacggtc acgagcttcc
     9841 tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat gacctttgct gcgtgagttt
     9901 gaagatgatg gtttcataat ggttcgtctt ttagccgttt tattcagtat agatttgcgg
     9961 cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag attactctgt cgcctttttt
    10021 cctgactcat aacccttaac gggtcttaca atccttgcag gccgccaaca caccctgttt
    10081 ttccattagc cggaacaatc cgtgttgatt ccatttccgt ttaattacgt ctccggacgt
    10141 ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc
    10201 attgttcctg ttagcgtcct cgctctatgg gtgctacttt acgcctttgc tttaaacaaa
    10261 caatcaaccg ccatcagtat ttgcacattt tcagagtttt atataaaagc aattcactga
    10321 tttatatagc ttatttgaca tgcatcgcag aattttctct gctatttcgc ataaagacta
    10381 tcggcaatgt ctcacaaagc gtatggcttt tacttacaac gctcacgccg aaaaatccgc
    10441 cttttattat ggttcaggta taatcgccac aaagctttga ctgacggaga cgaccgcttt
    10501 gactaatttg aattatcaac agacgcattt tgtgatgagt gcgcctgata ttcgccacct
    10561 accttccgat accggaattg aagtggcttt tgcaggccgt tccaacgcag gtaaatccag
    10621 cgcgctgaac acgctgacta accagaaaag cctggctcgt acctcaaaaa ccccagggcg
    10681 cacccagctt atcaacctgt ttgaagtggc tgacggcaag cgtctggttg acttgcctgg
    10741 gtacggttat gcggaagtcc cggaagagat gaagcgcaaa tggcagcgtg cgctcggcga
    10801 atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg ctaatggata ttcgccatcc
    10861 gctgaaagat ttggatcagc agatgattga gtgggcggta gacagcaata tcgccgttct
    10921 ggtgctgctg accaaagcgg acaaactggc aagcggcgca cgtaaagcgc aattgaatat
    10981 ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgtt
    11041 gaagaaacaa ggcgtagaca agctgcggca gaaactggat acctggttta gcgagatgca
    11101 gcctgtagaa gaaacgcagg acggcgaata attttcttgc cttaatgctt gtgccggatg
    11161 tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta ccgttggcct gataaacata
    11221 gcgcatcagg cattacggat cttttctttc gcccaataaa aaacgcccca gtcattactg
    11281 actggggcgg ctaaaatatt cagccaaatc cgattacgtg aagtaaaagg tctgaaagat
    11341 agaacatctt acctctgtac cctacgcgac taactttact cttttttgtt cagttcagaa
    11401 agcacttttt gtaatttttt ttcagttgtt gcataggaat ttctaatagc catcacaaaa
    11461 cgtgacagct tatgttgctt acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg
    11521 cctgatccca gttttcgcca ctccccactt ccaccagcaa cggcacatcc agacgggtac
    11581 agttttccat cagttgatga atctgcttcg cgacggcatc aacatcatct ttatgaactt
    11641 caaataccag ttcatcgtgt acctgcatga tcatacgtac acgcggttgc tcagcctgta
    11701 accacgcatc aacggcaatc atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg
    11761 gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc accattgctg gatttgatat
    11821 ccggcagata cagacggcgt ccgtccaacg tttcaacgta gccctgctct ttcgcctgag
    11881 cacgggtgcg ttccatatac tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt
    11941 acttctgcgc ttctttacgt ggaatgttca attgccgcgc cagaccgaaa gcactcatgc
    12001 cataaatcag accaaagttg atcgctttcg cgctacggcg ttgctcgctg gtgacggttt
    12061 ccagtggcaa accaaacact tctgccgccg ttgcccggtg gatatctttt ccttccgcga
    12121 atgcggtcag caagccttta tcacgcgaga gatgcgccat aatgcgcagt tcaatctgcg
    12181 agtagtccgc tgagacaatc acataatcct ctggcgcaat aaacgcctgg cggatacgac
    12241 gaccttcttc gttacgcacc ggaatgtttt gcaggttagg atcggttgac gataaacgtc
    12301 ccgttgcagt tactgcctgg tgataagagg tatgcacacg cccggttttc gggttgatca
    12361 tcagcggcag cttgtcggtg taagtcgact tcagcttcgc cagaccacga tactccagaa
    12421 tcacttttgg caacggatag tccagcgcca gttcttccag tacctcttcc gacgttgacg
    12481 gcgcgccacc cggcgttttc ttcagcggtt taataccctg tttttcaaac agaatggttt
    12541 gcaactgctt ggtggaagaa aggttaaatt cttcacctgc aatttcatgc gctttctttt
    12601 ccagctcagc cagacgcaag gtgagctctt cagaatgatt gtgcagcact ttcggatcga
    12661 tcttcacacc gttacgttca atgcgtgaaa gcaccggcac cagcggcatt tcgatattct
    12721 cgaagacgtt caacggccct ttgtgttttt gcagatccgg ccacattttc agatgcaact
    12781 gcaaggtgac atctgcatct tcggcggcgt aacgtccggc ctcttcgagg gcaatctggt
    12841 taaaggtcag ttgatttttg cctttacccg caatctcttc aaaagtgatg gttttgtgct
    12901 tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc ggcaacgcta ttgagaatgt
    12961 aggactccag catggtatca aacgcaatcc cacgcagttc aatgccgtaa ttcgccagaa
    13021 taccgcgatc gtatttcagg ttttgcccga ccttcagcgc cttttcatct tccagcagcg
    13081 gttttagcaa ctcgagtgca cgctcgtgag agatttgatc gggcgcatca agataatcat
    13141 gagcaaccgg aatatatgcc gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt
    13201 tagcagagat gttatcaagg ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt
    13261 ccagcttcgc aatccacgct ttcagtgttt cttcatcaag gatagtgacg tagttgtcat
    13321 aagaaatcac cgttgccgtc acttctggcg cttcgtctgc aacactggtt tcctgcggct
    13381 tcgcggctgg ttttgcccct ttggcctgta accatttgcc cgcttcgaca tcagcagtcc
    13441 agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc ttccgctgcc ggttgctgca
    13501 cttccagttg ttcacaggtc agctccagtt caacatcggt tttaatcgtc gccaactggt
    13561 atgagagata agcaacttct ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac
    13621 ggaagctcaa cccagcaatt ttttctggct cggcatacag cgtatccagc ccgccaagac
    13681 cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg tacgccagga atgttatcag
    13741 aggagtcacc catcagcgcc aggaagtcga tgatcaattc tggcggcacg ccgtacttat
    13801 tcaccacctc ttccggtccg aggatggtat tcgtcatggt attgataagc gtaatatttg
    13861 gcgtcaccag ctgcgccata tctttatcgc cagtgctaat cagcaccgga cgcccggctt
    13921 tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc ttctacgcca gaaaccgcca
    13981 gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg ttcgatttgt gctcgcagat
    14041 cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt
    14101 ttcccttggc gtcaaagacc accgctgcat gcgtcggttt atattgcatg atcagactgc
    14161 gcagcatgtt gaggacacca tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg
    14221 ggggaaacgc gtgatatgcg cgataaagat aagatgaacc atctacaagg ataagtggat
    14281 tttgggggat ctgaaccata atgtccgtgc ctgtttatca gattatgggt aaaggatgcc
    14341 acagacagga tgaaaacatg agtttttcac cgcttttcgt gaaaatcttt tgtagatctg
    14401 ctggatcgct cgcaaatcgt catgtggata actttgtgca taaattattt acatcgcttt
    14461 taatttatta tcatgctttt tacagcatga tgtttgtctt tgtttttcat gcagttaaaa
    14521 ataaatccat ccctaataat tgagataatg ggattttttc taatgtggat agatatgaat
    14581 tatttttctc cttaaggatc atccgttatt tgggtcattt tctgctaaaa tctgcgcctt
    14641 gcgcttcttt agtgcactgt ctatggctaa ccttttgaat aaattcatta tgacgagaat
    14701 actcgctgcg ataacccttt tgctgagtat cgtattgacc attttggtca ctattttttg
    14761 ttccgtcccg atcatcattg ccgggattgt aaaactattg ctacctgtgc cagtcatttg
    14821 gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc tggtgtgaag gtctggcggt
    14881 attactgcat ctgaacccac acctgcaatg ggaagttcac gggctggaag ggttaagtaa
    14941 gaagaactgg tatctgctta tttgtaacca ccgtagttgg gcagatattg tcgtactgtg
    15001 cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc ctcaagcaac agctggcctg
    15061 ggtgccattt cttggcctgg cgtgctgggc gctggatatg ccgtttatga agcgttattc
    15121 ccgcgcttat ttattacgtc atccggagcg acgtggtaaa gatgttgaaa ccactcggcg
    15181 ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc aatttcgttg aaggctcccg
    15241 tttcacgcaa gaaaaacatc agcaaaccca ctccactttt caaaacttgt tgccaccaaa
    15301 ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa caattcgata aactgcttaa
    15361 tgttacgctg tgttatccgg acaataaccg tcagccgttc ttcgatatgt taagcggtaa
    15421 actgacgcgg attgtcgtac atgtggattt acagcctatt gccgatgagt tacatggcga
    15481 ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa tggctgaact cattgtggca
    15541 ggaaaaagac cggcttctga cgtctctcat gtcatcacag cgtcagaata agtaagtttt
    15601 actcgcctct gaatgagcag aggcgagtga gtattttaat gaacaaaacg tccggcacga
    15661 gacataaatt cttcttcgct catctcctgt ccgttaaaaa caactttacc cggtgtataa
    15721 cgcagctgca gcgaggcggt attgttgtca atcgtgatta actgaaacat ctgacctaat
    15781 gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg ctttttccgc atccattcct
    15841 tcagataaat taagctgttt tgcggtttct gtgacgacat taagcggtaa cttcacatca
    15901 aaattgagcg atttgatatc tttgtttgtg gatgatgaag atttggctgg gtcggcaata
    15961 ctgatatcca gattggcatt tagttcgccg agtgcgttct tccatcttac cggttgttta
    16021 atggtcggct cacttttttg taataacggt aaatactctt tgaacaatgc agcattcact
    16081 tcttgcagag caacttcatc gtttgctaac tcagggtgtg cagcaagctg cttctgcatc
    16141 gcaatgttat actggatgat aaattggcgc actgctgagg gatcaataga ttcagcaatc
    16201 aatgaccact caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag
    16261 gtcagattga cattgatata atctttgtct ttaacgcgat ccagcctgtt ttttgcaacg
    16321 aaagcatcga tttgggcaac gtcttccccg tgattgataa tgttaatctg attaaatttc
    16381 gactcgcttt caccaaccgg aaatttcttt tcttctaatc gcgccagaga atcaatggta
    16441 aaactttttg ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg
    16501 ccttcaatat ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca
    16561 ccaaaagaaa actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca
    16621 ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc
    16681 ggttgtgtca gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca
    16741 ttaccatgca ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaataca
    16801 actttctggc ctggcttgat attgagatcg ggtgcatcgt tatcgaatgt catttgcatt
    16861 tgaaaacggg aattaaaaaa gcctttgtca aagtttttat aacttaacgt catatcgtag
    16921 gcatgttcac ctttcttttt cgcatcgtta aaatctttaa taaacttttc gataccaggc
    16981 tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga ttacggctaa cgcaacaata
    17041 acacctgtag ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttga
    17101 atatccactt atgctgaaat aatgtccagc ggcaggatgc attatcagaa tgtatttata
    17161 tttattaaat aataaaaaaa gcccgtgaat attcacgggc tttatgtaat ttacattgaa
    17221 ttattttttc tcggacagat atttcaccgt atcagcatac tgctgaacaa aaacatccat
    17281 attgctggta tccataccct gcggattcag ctgatattta ccgttaacaa acatcgccgg
    17341 aacaccacgc aattgcacat cagctgcggc tttttcctgc tgagcgacca gagatttcac
    17401 cacgaagctg ttccacgccg cgtcgtactc ttcaccttta atacctgcat tgataaatac
    17461 atcgcggata tcagatgctg aacgaatggt ctgggttttc tgtacgcctt caaacagcgg
    17521 aacagtcact ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc
    17581 tttgcccagg tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg
    17641 cagttttttc ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg
    17701 gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt
    17761 agtgtactgt ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc
    17821 agccagcgcc agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta
    17881 attgcaaagg gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtctgtcgta
    17941 gccagtaatc ttccccggtt aaccacggga aatttttcgg gaacgcggga tcagcccaat
    18001 gccgcattag ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc
    18061 cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt
    18121 gcatccgctg ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac
    18181 cattacgtgc atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg
    18241 cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc ccgccagtgt gctgtaacgg
    18301 cggcgatcag ctcatctgtc gctttcagaa atgccgcttt caacccggaa ggtatcagtg
    18361 tagcgtcctc aaacagcttg cgaggctcaa tgagatattc attcagaccg atggtcgggc
    18421 gatgaataaa aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg
    18481 cttccatctg atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag
    18541 cgaaataaaa tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga
    18601 ccggaacttc atcatttacc agctgcaacg caaattgatg ttcttcgagg atttgatcgg
    18661 ctgtccaacg ttcagggcga taaaatttga cgacaaaacg tcgacgctct tcgtcctgaa
    18721 attgatagac acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc
    18781 cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc
    18841 tgttgttcat cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg
    18901 attttggtgg cgccgcttac aagcttttac tctttaatta cgccgcgagc gcgtaacagc
    18961 gccgttttga agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg
    19021 gaatcacgca ttttcaggtg atagatcaga atgtcatccg tcaaatccgc cagttcgccg
    19081 tcaaaacctg actctttcgc cagtttctgc aaaaattgca acaagttaaa gtctggctct
    19141 ttttgccagg ctggctggag gagttcaata acttcattca gacgtttaca tttcatagta
    19201 atgctcctta ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga
    19261 tatcggtgaa tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac
    19321 gaatgggcgg cgtagataaa ggattgctgg acttaaatgg caaaccgctg tggcaacatg
    19381 tcgctgacgc gcttaagacg cagctctctc acgtcgtggt taatgctaat cgtcatcagg
    19441 aaatctatca ggcaggcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc
    19501 ctctggcagg aatgctttca gtaatgcagc aggaagctgg tgagtggttt ttgttttgtc
    19561 cgtgcgatac gccttacatt ccccatgatt tagccgcccg gcttaatcat cagcgcaaag
    19621 atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca cccgactatt gctctggtaa
    19681 accgcgctat tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg
    19741 catttatgcg tctggctggc ggtcatgcgg ttgatttcag cgatcataaa gatgcatttg
    19801 ttaacgtgaa tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact
    19861 cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt ttgaaaaaac tgatcccggc
    19921 attatgcgcc agagggatcc gtccggggct gattaagcat acgcaccatg atatggatgt
    19981 tgataagcca ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt
    20041 tgccagccag caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct
    20101 acattttctc gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt
    20161 taagcatgaa gagatcgcaa agattgtgct gtttcgcgat ggagccggac atcgacctga
    20221 agaattagtg atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga
    20281 tgtcgcatta ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat
    20341 gcaaaagcag aacggataag caagagtagg ctggatcaga cgcagccagt gtcgcatccg
    20401 acctttttct gcaccagcgc aaaaaggccc gactgggcct
//
LOCUS       DADKXM010000064        20369 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.064, whole genome shotgun sequence.
ACCESSION   DADKXM010000064 DADKXM010000000
VERSION     DADKXM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.064"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(178..2499)
                     /locus_tag="JQR05_002307"
     CDS             complement(178..2499)
                     /locus_tag="JQR05_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY5324471.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     GTERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLVPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JQR05_002308"
     CDS             complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JQR05_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY5324472.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            3300..4019
                     /gene="ompR"
                     /locus_tag="JQR05_002309"
     CDS             3300..4019
                     /gene="ompR"
                     /locus_tag="JQR05_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY5324473.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4016..5368
                     /gene="envZ"
                     /locus_tag="JQR05_002310"
     CDS             4016..5368
                     /gene="envZ"
                     /locus_tag="JQR05_002310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY5324474.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5444..7066)
                     /gene="pckA"
                     /locus_tag="JQR05_002311"
     CDS             complement(5444..7066)
                     /gene="pckA"
                     /locus_tag="JQR05_002311"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY5324475.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNTVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7445..9169
                     /locus_tag="JQR05_002312"
     CDS             7445..9169
                     /locus_tag="JQR05_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY5324476.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWALWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9304..10182)
                     /gene="hslO"
                     /locus_tag="JQR05_002313"
     CDS             complement(9304..10182)
                     /gene="hslO"
                     /locus_tag="JQR05_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY5324477.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEMPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10207..10608)
                     /gene="hslR"
                     /locus_tag="JQR05_002314"
     CDS             complement(10207..10608)
                     /gene="hslR"
                     /locus_tag="JQR05_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY5324478.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALARKMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10619..11287)
                     /gene="yrfG"
                     /locus_tag="JQR05_002315"
     CDS             complement(10619..11287)
                     /gene="yrfG"
                     /locus_tag="JQR05_002315"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY5324479.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWSAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11352..13487)
                     /gene="igaA"
                     /locus_tag="JQR05_002316"
     CDS             complement(11352..13487)
                     /gene="igaA"
                     /locus_tag="JQR05_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY5324480.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPSPTGASAAPLSLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATGVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13807..14367
                     /gene="nudE"
                     /locus_tag="JQR05_002317"
     CDS             13807..14367
                     /gene="nudE"
                     /locus_tag="JQR05_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY5324481.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFYEARNVSALFLVREWLKGQGRV"
     gene            complement(14532..17084)
                     /gene="mrcA"
                     /locus_tag="JQR05_002318"
     CDS             complement(14532..17084)
                     /gene="mrcA"
                     /locus_tag="JQR05_002318"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY5324482.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITHKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLGQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17204..17983
                     /gene="hofM"
                     /locus_tag="JQR05_002319"
     CDS             17204..17983
                     /gene="hofM"
                     /locus_tag="JQR05_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY5324483.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            17983..18522
                     /locus_tag="JQR05_002320"
     CDS             17983..18522
                     /locus_tag="JQR05_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY5324484.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18506..18946
                     /gene="hofO"
                     /locus_tag="JQR05_002321"
     CDS             18506..18946
                     /gene="hofO"
                     /locus_tag="JQR05_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY5324485.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18936..19340
                     /gene="hofP"
                     /locus_tag="JQR05_002322"
     CDS             18936..19340
                     /gene="hofP"
                     /locus_tag="JQR05_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY5324486.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNYVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19252..>20369
                     /gene="hofQ"
                     /locus_tag="JQR05_002323"
     CDS             19252..>20369
                     /gene="hofQ"
                     /locus_tag="JQR05_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY5324487.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQ"
BASE COUNT         4609 a         5284 c         5541 g         4935 t
ORIGIN      
        1 ttaattaagg tttcagttgt taatgaagtg ataaatatta acggagtata ttgtgagttt
       61 ggctgtggaa taaaagtgcc ggagatattc tccggcactt ttatgtcagg caaagcgttc
      121 gttgccagat gcggcgtgaa cgccttatcg cgcgggttta ggggccagtc cgggcgttta
      181 acgtttttta cccatcgctg ccgccagcgc atccatcatc gcgctattac cggcaggctg
      241 cgcttcacga ccgcgtggtt tggctgccgg gcggttgttt tgcgggcgtt cattaccgcc
      301 gccgcgacga gagttggttt cgccaggctg ctcgtccaga cgcatagtca gggcgatacg
      361 tttacgctga agatccactt ccagcacttt caccttcaca atgtcgcccg ctttcaccac
      421 ggtatgcgga tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg
      481 atgcacgcca atatcgacaa acgcgccaaa gttggtgacg ttggtcactg cgccttcgag
      541 gatcataccc ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt
      601 aaattccgga cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt
      661 cggcacaccg aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct
      721 gttacccatc agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg
      781 ataggcttcc ggatgaacgg tagaggcgtc cagcgggtta tcaccgtggt taatgcgcag
      841 gaagcccgcg cactgctcga aggctttcgg caccagacgg ctgactttca gcagttgctg
      901 acggttctgg aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg
      961 cgtcaggccc gccacgcggg ttaatagcgg aacagaagcg gtgttgagat cgacgccaac
     1021 ggcgtttacg cagtcttcta ctactgcatc cagtttgcgg gccagttgcg tctggctgac
     1081 gtcatgctga tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag
     1141 cggatcctgc aaacggcggg cgatagatac cgcaccacgc agcgaaacgt cgagatccgg
     1201 gaactcctgc gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat
     1261 cactttctgt gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt
     1321 tccgcgggaa gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa
     1381 ggcagcaacg gtcatcgctg cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt
     1441 cgccaccagt ttgccagtgg catcgaccac cgccactttt accccggtac gcagacccgg
     1501 atcgaggccc atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt
     1561 acgggcaaag acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc
     1621 ggtttccaga tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca
     1681 gctatccgcc ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca
     1741 atagctctct ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac
     1801 gccttcgtta cgcccacgga acatcgccag cgcgcggtga gaaggcaccg tggacaacgg
     1861 ttcgtgatga tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac
     1921 cgtagaaacc aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc
     1981 atcttcggca aaccgttcca tcaggatata gcgcgcgcca tccagcgcgg ctttggtatc
     2041 tgccacgcct ttatcggcat caacatattg tgcagcggcg acttctggcg tgtgtgacgg
     2101 atcgctccac agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg
     2161 ggtgcggcgt ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt
     2221 ggcgttgatg gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggatttgag
     2281 gatcgcctga cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg
     2341 cagctgcgtg tcatccagac cgccggtgat ttccttacga taacgtgcga taaacggcac
     2401 ggtattccct tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat
     2461 ttcacccgca ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg
     2521 atcaaaaatc aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga
     2581 ggttaagact cttccttacg gtttcacgta ctcgatagca ttaacatacc agttcgcttc
     2641 cccggcaggg gtattcacca ccgccagatc gccgacttct tttttcagca atgcgcgggc
     2701 catcggggaa tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat
     2761 acggaaacgg tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac
     2821 tttgccttcc tgctgagggg agtaatcgac gattttgaga ttttccaggc atttagtgag
     2881 atagcgcacg cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt
     2941 ttcgctgcgg tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc
     3001 ttcacgccag agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag
     3061 gggcgttttc atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac
     3121 gtgatcatat caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg
     3181 tttaatatgc tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac
     3241 gtgagctttt ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa
     3301 tgcaagagaa ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg
     3361 aacgttatct caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg
     3421 atcgcctgct gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg
     3481 aagatggctt gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta
     3541 tggtgacggc gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg
     3601 actacattcc aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc
     3661 gtcgtcaggc gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg
     3721 gtaagttcaa acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc
     3781 tcaccagcgg tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct
     3841 cccgcgataa gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca
     3901 tcgacgtgca gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt
     3961 acattcagac cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag
     4021 gcgattgcgc ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt
     4081 gctgttcgcc agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag
     4141 cctccagcag tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact
     4201 gcaactggag gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg
     4261 tgagctgggg atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc
     4321 gcaacactat gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt
     4381 gcgcgttgag gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa
     4441 tatctgggta cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg
     4501 ctatacgctg gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca
     4561 gaaccgaccg ttggtcgatc tcgaacacgc agccttgcag gttggtaaag ggattattcc
     4621 gccgccgctg cgtgaatatg gcgcttcgga ggtgcgttcc gttacccgtg cctttaacca
     4681 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag
     4741 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca
     4801 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga
     4861 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa
     4921 tgcagtgctc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc
     4981 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc
     5041 gaatatggtg gtcaacgccg cccgttatgg caatggctgg atcaaagtca gcagcggaac
     5101 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca
     5161 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac
     5221 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct
     5281 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg
     5341 ggcgcagggc acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgttttg
     5401 ctttctataa gatactggat agatattctc cagcttcaaa tcattacagt ttcggaccag
     5461 ccgctaccag cgcggcaccc gcaggggtgt cggtgtattt atcgaagttg tcgataaaca
     5521 gtttcgccag ggtttcggct ttttcctgcc actgttccgg agaagcgtag gtgttacgcg
     5581 gatcgagaat cttcgtgtct acgcccggca gttcggttgg gatcgccagg ttaaacatcg
     5641 gcagagtgaa ggtttctgca ttatccagcg aaccgttgag gatggcgtcg ataatggcgc
     5701 gggtatcttt aatcgagata cgtttgccag tgccgttcca gccagtgtta accagataag
     5761 cctgcgcgcc cgccgcctgc atacgtttca ccagcacttc tgcgtactga gtcgggtgca
     5821 gcgacaggaa cgccgcgccg aagcaagcgg agaaggttgg cgtcggttcg gtgatgccac
     5881 gctcagtacc ggccagtttg gcggtgaagc cagagaggaa gtgatactgg gtttgatcgg
     5941 cagtcaggcg agaaaccggc ggcaacacgc cgaaagcatc agcagtcagg aagataacct
     6001 tagtcgcgtg gcccgctttg gaaaccggct taacggtgtt atcgatgtga tagattggat
     6061 aagaaacgcg ggtgttctcg gtttttgaac catcatcaaa gtcgatagtg ccatcttcac
     6121 gcacggtgac gttttccagc agtgcatcac gacggatagc gttgtagatt tcaggttccg
     6181 cttctttcga cagcttgata gtttttgcgt agcagccgcc ttcgaaatta aacacgccgt
     6241 catcgtccca gccgtgttcg tcatcgccaa tcaggcggcg tttcgggtcg gtggaaaggg
     6301 tggttttacc ggtgccggaa aggccgaaga acaccgcaac atcgcctttc tcgccaacgt
     6361 tggcggagca gtgcatagaa gcgatacctt tcagcggcag caggtagttc atcatcgaga
     6421 acatcccttt cttcatttcg ccgccgtacc aagtgccgcc aatcagctgc atgcgctcgg
     6481 tcaggttaaa cgccacgaag ttttcggagt tcagaccctg ttctttccac tgcgggttag
     6541 tgcacttcgc gccgttcata acgataaagt ctggtttgaa acctgccagc tcttcatcgc
     6601 tcgggcgaat aaacatgttt ttgacaaaat gcgcctgcca ggccacttcg gtgatgaaac
     6661 ggacggaaag acgagtatcc gggttcgcac cacagaaagc gtcgacaacg aacagacgtt
     6721 tgccggaaag ctgcctggtc accaggcctt tcagatgctg ccaggtttcc ggagagagag
     6781 gtttgttgtc gttcttacct ttgcctttgt ctgcccacca gaaagtatcg cgagtggtat
     6841 cgtcacggac gatatactta tcttttggtg aacgaccggt gaagatcccg gtatcgacgg
     6901 caacggcacc cagattagtt aacaccccgc gctcataacc tgtcaggctc ggatcgagct
     6961 cttcctgata cagcaggtcg tagcttgggt tgtaaacgat atcatgtacg tcactgatac
     7021 cataagcctc gagttcttgc ggggtcaaac cattgttaac gcgcatttca ctgctcctta
     7081 gccaatatgt attgcctgaa tagtaaagtc tttttggggg tgttaaccgc gacaaggctc
     7141 atagatttac gtatctggag aaattcatat ttgacaaaaa actccgtgat tcctgtcacg
     7201 aaacggttgc tattatcgca gaaatagcat tttaggtgtg gaaaatgttc tcaaaaggtt
     7261 aaatcttggt ttatatggcg ggaatattgt gagtggaatc gcattcctga atgaaattga
     7321 tgcaaattca accggtaaag tatcgattca ccacgctaac tttttttgat tattttctga
     7381 aaaaagcaaa atactactgc ccggatgcga taattaaccc accttttgat tctggataac
     7441 caacatggat aacgttgaac tttcaccggc aacacgctgg gggatgattg ctaccggatt
     7501 acttcagggg ctggtttgct acctgcttat cgcctggctt gccggaaaaa atcacagttg
     7561 gattgtttat ggcgtgcctg cgaccgtcgc gttttcatcc gttttactct tctccgtgat
     7621 ctcttttaaa cagaagcgcc tttggggatg gctggcgctg gtgtttattg ccacgacagg
     7681 gatgagcggt tggctgaagt ggcaaactga tggcatgaac ccctggagag ctgaaaaggc
     7741 gatttgggat tttggctgct atctgctgtt gatggcaatg ttgttgctac cgtggataca
     7801 acaaagcctt cgtatccgca atggcagtag ccgctatagc tacttttacc agtcagtatg
     7861 gcataacgta cttatattac tggtgatttt tctctccaat ggcctgacgt ggctggtgct
     7921 tttactgtgg ggtgagttgt ttaaacttgt tggcatcacg tttttcaata cgctcttttt
     7981 tgcaaccgac tggtttatgt atcttacatt tggtctggtt accgcactgg cggtgatcct
     8041 cgcgcgtaca cagtcacgtt taatcgactc tattcaaaag ttgttcacgc taatcgccac
     8101 ggggttgctg ccgttagtat cattgctaac cctgatgttt atcatcaccc tgccgtttac
     8161 gggcctgagc gcgatttctc gccacatctc cgccgccggg ttgctgttaa cgctggcctt
     8221 tttgcaattg atcttaatgg ctattgtccg cgatccgcaa aaagcgtcac ttccctggac
     8281 ggggccgttg cgttgcctga ttaaaaccgc tttgctggtt gctccgctgt atgtgttcgt
     8341 cgccacctgg gcgttatggc tgcgggtcgc tcagtacggc tggactgtcg accgcttgca
     8401 gggcgcgctg gcggagctgg tgttactggt gtggtcgctg gggtattttg tcagtatcgt
     8461 ctggcgtaat gggcaaaatc cccttgttct tcagggcaaa gtgaaccttg cggtttcgtt
     8521 attggtgttg gtgatactgg tgcttcttaa ttcgccggtg ctggacagta tgcgtattag
     8581 cgtgaacagc catatggcgc gttatcagag cggcaaaaac acgccagacc aggtaactat
     8641 ctacatgctc gaacagagcg gtcgctatgg acgtgcggcg cttgagtcgc tgaaaagcga
     8701 tgccgggttt atgaaagacc cgaaacgcgc gcgggatctg ctgatggcgt tagatggaga
     8761 gcaacatctt caggaacagg tatcggaaaa agtattagcc gaaaatgtgt taattgcccc
     8821 tggttctgtt aaaccggatg cgacattctg gtcggcctta atccaggatc gctataacgt
     8881 gatgacctgt attgaaaaag acgcctgcgt actggtcgag caagatctga atagtgatgg
     8941 tcaggcggag cggatcctgt ttgcttttaa tgatgacaga gtcattgtct atggctttga
     9001 ctcagacaga aaagaatggg acgcgcttga tatgagttta cttccgaacg aaataacgaa
     9061 agaaaaatta ctcacagctg cgaaggatgg aaaactgggg acgaggccga aagcgtggcg
     9121 cgatctgacg gtagatggtg aaacgctgga aataaactta agtaagtgac aacgctttta
     9181 tcggatacga cgctgtatct gaccaaaata atttgcgatt tatttcatag gcgctggtaa
     9241 gcgcagcaca tcaggccatt aagtcgtaaa atccggcaga gccctctctg ccggacatac
     9301 tcattaatga acttgcggat ctgccggaga cgcgttgttg cggatttcag caatatccat
     9361 cgcattgaac agatagtggt taccgcagta atcacaatgc atgtcaattt cgccatcttc
     9421 cgccaggatg ctatcaactt cttcatcagg cagcgttttc agcgcatcgg cgcaacgttc
     9481 acgcgagcag gtgcatttga actccacatc ctgcggatcg taaaccgtca cctcttcttc
     9541 gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc agcagttctt cggttttgat
     9601 ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc tgggtatttt gcgcaggcat
     9661 tacctgcaac aacataccgc ctgcagccgg tttgccgtct acgtcgccgg tgcgaataaa
     9721 caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct tccaggcagg ccgccagggt
     9781 atcaccttcc agaccaacta cgccctgata gcgttcgcct tcgctcgggg taatggtgat
     9841 caccacgtaa ccattgccga ccagcgtttt caggtcggca ttttctggca tttcgccctg
     9901 cacgcgcgcc acaccgcgca tttgctggtt attgttaccg ttaataaccg ccagattcat
     9961 cggaccgtcg ccctgcagct gtacggtgat atcaccatca aacttcagcg tagcggttaa
    10021 caggctggtc gcaaccagca gttctgccag cacgttttta acgggctgcg gataatcgtg
    10081 gttctcaagg atctgttgca gggtttccga aacggttacc agttcgccgc gaacggcaaa
    10141 attttcaaac agatagcgat gtaattggtc atgttgcggc ataatcatct ctcttgcagg
    10201 tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg tcgcggcgct cttttttgtc
    10261 cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt ttacgtgcca tcgccatttt
    10321 ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc aaggctgcct cgctggcggg
    10381 gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt tcgtcatttc cctggcgcag
    10441 agtgagcgtg gcattcagct cgacgatttt gctcggcttg ctgcgctgcc cgttgtaatg
    10501 caccttaccg ccttcaatca ttttacgggc cagcgcgcgg gttttataaa aacgggcagc
    10561 ccatagccat ttatccagtc gaacctcaac agcaggtttc tctttcatgg cgtctccttc
    10621 acattagcga ggggatcagg cggcggtagt cattcagtga cggatggcgt tgatactgtt
    10681 tctcggcaat cccggaatca ggattagtca cgccgaggca gtaacgaata ccaaattgcg
    10741 cggcagcatc gagaatcgct tcgctgtcat caataaacag cgttctttca gctttcagac
    10801 ccgtagcttc ggccaccgca tgccataacc gctgatcctc tttcggataa ccaaatgtgt
    10861 gggtggaaag taataaatca aggtgtgcgt ccagacccgt atgctcaagt tttaccgcca
    10921 ggttgtgcgg atgagcattg gtgagcaaaa ttcgctgctt accgctggct ttcagtgcct
    10981 caagaaacgg aatggtatct tcacgcagta cggcgcgcgg tcccatctcg gtggtcatcg
    11041 cacagatatc cagacccagt tgctcactcc agtaatcaag acagtaccag tttagcgtat
    11101 gctgtacgtc gtgatattgc tggcgcatat attccatcgc ttcctgtggc gtaaccccgt
    11161 ttttcgcgct ccatgtttca ggcaccagct tttgccagaa atagttatcg aaggcgaggt
    11221 cgagcaacgt gccgtccata tccagcagaa cggtatctac gtcctgccag gcaatgttga
    11281 tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg acagggtagc ataacctgcc
    11341 gcgcaaacgt gttattcgat aaggctttct gaaggggtga tcagttgcgg gttcaggcag
    11401 ctttcataat aggcctgaat ctccatcatg cgagtgcgat gacgctggta acgacgccag
    11461 gcctgtacgc cattgtaaat ggcgctaccc agcatcgtca gtagcagcaa tgtggtgctg
    11521 agatagcgcc acaggccgga acgatccggg atcggatgaa ggccaatatg ctgcgtacca
    11581 ttggcgtcag tgaagatttt tgtgacgata ccttcggcgt taaacggcgt atgcatcagc
    11641 atttgtgcca gtttctggaa agcgttccac tgttcttgcg gcgggtagtc gtaaagtgat
    11701 gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt cgctgacaat caggaatccg
    11761 ccgggggccg gactgtttag tgattgtgcc gctcgcgccg tttcatgcgt gataaacggc
    11821 gcggtggagg ttgccaccag gttatccagc gattccgcac tcaccgggcg taataacaca
    11881 ttcacgccat cgagcttccc ggcgttggcg cgttttacca gcgcgtccca gtctttactg
    11941 ttgccgagat tgaccagcgc atttttcagt cgcacacagt catctttggc agaacataaa
    12001 tccgctgtct tcagtacaat gtcgccaaaa tcatcaagca ataccatgcc ggatttttga
    12061 atcgctgaac gtaatgaggc actgacgcga gattcatctt ccggtttagg gtgcagctgg
    12121 cgattaactg cttcagtcaa tgccgtcgct ttgttgacca gttcagattc tggtaatggt
    12181 aatgagcggg cgtcattcca gatgatctgc gagcagtcaa acggtaaaaa aggtgaattg
    12241 gttttcgcgc tccaggttcc ggaagtgcga atattacaca ttcccgtacc gctaatacgc
    12301 aatgtatcgc ctacccgcac gccagcgtca gccagttgtt ttacgccggt ggcttcaatg
    12361 gtctgcgcgc ctttcatcca tgagagagtg aatttcagcg gcatatccag cgggatccag
    12421 aataacagca taaacagcac cagcagcgag cccgcagcga taatcgtact gcgcagccag
    12481 tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa gatatcgccc ctgacgcact
    12541 acgtggcggt cgagatagat atcaatatcg gtttgttgac cgagatcctg agcaatgtat
    12601 ggctgccagt gtgcgggata gaccaggtcg ataataccga gtgaaatatt gttgatctgt
    12661 tcctgatcgt tttcgccaaa caatccccaa cgacggggtg taccgcgcag acaatgaatt
    12721 tctcgcaggg aggattttgc cgggggcgcg aatagccccc acagacctgc gcccagcagc
    12781 agtaatgcgc cgcctgccag ccacggaaca aatacatccg gggtaatcag gcagaaaaag
    12841 aacatcagga aggaggcgac gatcagcaac gcttcacgca gcccgcgcgg acgactcagc
    12901 gcatattctt catgcgtttc tttgcgaata ttgagtagct cgatttgctc actctcttcc
    12961 ccgcgaatcg acgcctgcgt ggacggaaca ggttgcaagg catagccgcg cgtttcctgc
    13021 atgtactcct gcagcgtatg accgttgagt gaaataacca acggcagcga atcggtatga
    13081 atcagttcaa cggtattctc atcgttgatg tactgttccc agaagggggg caggtggact
    13141 tctaccgaat cgaggtagta acgccattta ttcggatcgt cggtagagat gccgtaacgt
    13201 gtgatagcgt gtgtcagcat catgacgttg ttgctttcgg cattcagcgc cagagagagc
    13261 ggtgccgcgc tagctcccgt tgggctaggc acctgtaata cctgcgtcag gctctcaaga
    13321 taattttcaa cggcgctacg ttcttcaggt gtgagtttac gcgtttgcgc atccgcgaag
    13381 gcgttggtcc agggcagctg acgccgtctg gatcgcactt ttatcagcca tcccgcaagt
    13441 agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg tgctcatgct ttccccatct
    13501 tacttagtct gtcaggcgtg gtcagtcgca ccggacacaa tacatgattc tgcggttggc
    13561 tatcggcaag cgtggagttt aacttgagca tcattaagca catcacagta accgctgatc
    13621 atagcaaacc ctctgaaggc ggaatatcag caaattcctg gagttatttc aacctattga
    13681 cttttaattt gatttgagga cgattattta ttaagttaca taaatgtaaa taacatgcaa
    13741 ttcacattgc cgaaagcgtg tccgatatcg cacaataacc gctgatttca cagcatagat
    13801 cccacgatga gcaaatcatt acaaaaaccc accattctga atgttgaaac tgtagcccgt
    13861 tcccgactgt ttaccgtcga gagcgtggat ctggagttca gcaatggcgt gcggcgtgtt
    13921 tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga ttgtgccgat tgtggacgat
    13981 cacctgatcc tgatccgcga atacgcagtg ggaactgaat cctacgaatt aggtttttcg
    14041 aaaggattaa ttgatccggg tgaaagcgtc tacgaagccg ctaaccgcga gctaaaagaa
    14101 gaggttggat ttggtgcgaa cgatctgact tttttgaaga agctcagcat ggcaccgtct
    14161 tacttttcca gcaaaatgaa tatcgtggtc gcgcaagatc tctacccgga atcgctggaa
    14221 ggcgatgagc cagagccgct accacaggtg cgctggccgc tggcgcatat gatggatttg
    14281 ctggaagacc ctgacttcta cgaagcgcgt aatgtcagcg cgctgttcct cgtgcgcgaa
    14341 tggttgaaag ggcaggggcg agtgtaaatg cgtgaagttt gcctgatgca caacgcttat
    14401 caggcttatg gggttcctgc aatatattga tttttcacga ttttgttagc cggataacgc
    14461 gttcacccgc atccggcata aacaaatgca ctttgtcagc caactgaaaa ggcgccgaag
    14521 cgccttttta atcagaacaa ttcctgtgcc tcaccattat cgataatggt cgttcccacc
    14581 tcgtgcaccg cctgttgtgt cggctgcgta ccttcgatga aatactcttc gcggctgttg
    14641 ccaccattag ctaactgccc ggtgctgcga tcgatattca ccgtcacaat acccggaggc
    14701 ggcgtcagcg gctgctccgg cacaccttca agaacggctt tcatgtaagc gtcccatgca
    14761 ggctgggcac tcttggcacc gccttcgtaa cctgagatct gatctttaat cgctccggaa
    14821 gccgttgtat gaccgagatt acgacggtga tcatcaaagc caatccagac cgaggtcaca
    14881 acgcccggac cgtaacccga gaaccacgca tctttcgaac tgttagtggt accggttttc
    14941 ccgccgatat cgcgacgctg caaatcacga cctgcacgcc agccggtacc ctgccagcct
    15001 ggttcaccaa agatattggt gttcaaagca ctcttaatca ggaatgccag cggagtgttg
    15061 atgacgtgcg gtgcgtactc ctgcgcgcca gtcttcgcca ctaacgcctg atttgcctgc
    15121 cccagctgcg gcattggtac agaaacattc tgctgctcgc gggagatagc gacatcttca
    15181 acatcgttat tttccagcac gttcgatttc tgcgtatcac cgtaaatcac tggaatatcg
    15241 cattccgggc aggctacttt cggtttcgct tcgaaaatca cgccgccctg atcgttttca
    15301 attttgctga taaaccacgg gtccaccagg aagccgccgt tcgccatgac cgcgtagccg
    15361 cgcgccacct gcattggggt gaaggacgct gaacccagcg ccagcgattc ggtgtggaca
    15421 atgttttgcg ccgggaagcc gaagcgttgc agatattctg cagcgtagtc gacgcccatc
    15481 gcccgcattg cacgtaccat caccacgttt ttcgactgac ccagcccctg acgtaagcga
    15541 attggaccag catactgcgg tggtgagttc ttcggctgcc agtcagaacc ggcacctgca
    15601 tcccagcgag aaattggcac atcgttcaac atacttgcca gcgtcagacc tttatccatc
    15661 gccgcggtgt agaggaacgg tttgatgttg gaacccacct gacgcagtgc ctgggtggcg
    15721 cggttaaact tgctctgatt gaaatcaaag ccaccgacca gcgccataac ggcaccattt
    15781 tgcggattga tcgacaccag cgccgagttc acttccggca cttgtgccag ccaccatgca
    15841 tcgccaacct gacgaaccca gatttgctga cccgtttgca gaacatcggt cactttacgc
    15901 ggcgtcggtc cctgctgagt atccgaacgg taaggacgcg cccagcgaac gccttccata
    15961 ctcaatgcga cggtcgaccc gtccgccagc atcgccgtcg ctccctgagg attggcgctg
    16021 gtgactgcgg caggcagcag cggaccatag gtcggcagcg ccttcagcgt atcggtaatc
    16081 ttgttgttat cccacgccga ctcgcccact ttccacagca catttgccgg gccgcgatag
    16141 ccgtggcgca tgtcgtagtc cagcacgtta ttacgtaccg cctgctgcgc ggcctgctgc
    16201 actttgtggg tgatggtggt gtaaatgcga taaccgtctt cataggcact ttcgccataa
    16261 cggttataca tctcctggcg caccatttcg ctcaggtacg gcgcagagaa agcaatctcc
    16321 ggcgcgtgat aattagcgtt aatcgcctca gtgcgtgtct gatcgaactg ttgctgggtg
    16381 atataccctt catccagcat ccgcgacagt acgacgttac gccgcgcgac ggcacgatcc
    16441 attgagtaga gcggattaaa ggtggaaggc gctttcggca acccggctat caccgccatt
    16501 tcgttcagcg tcagttggtc gaccgtttta ccgaaataga cttgtgccgc agcaccgaca
    16561 ccataggcgc ggtaaccaag gtaaatcttg ttcagataaa gctcgaggat ttcgtctttc
    16621 gtcagcaact gttcaatgcg aatcgcgagg aagacttcct taatcttacg catcagcgtg
    16681 cgttctggac tgaggaagaa atttctcgcc agctgctggg taatggtact cgccccttgt
    16741 gacgcgtggc cggagaacag cgccacactt gccgcacgga agatccccac cgggtcaacg
    16801 ccgtgatgct cgtagaagcg gctgtcttcc gtcgcgataa aggctttcac catctccggt
    16861 gggatttgat ccaacgtaac cggaatacga cgtttctcac cgtattgagc aatcagctcg
    16921 ccatcggcgc tgtaaatctg catcggaatt tgcaggcgaa catcttttaa tgtcgccaca
    16981 tccggcagtt gtggctcgat gtagcggtat aggccataaa tcgagcctgc tcccagcaga
    17041 atgcaacaga ctgcaaggat caaaaaatac tttacgaact tcactggaaa tttcccattt
    17101 agtttcattt gggcagttta taaacaaacg cgcggtagta taaaggcaag ccaggcgcat
    17161 tgatataccc gtcagagtta cgggtgataa ggagatcatc acaatggcat ttaagatctg
    17221 gcaaattggt ttgcatttac aacagcaaga agcggcagcg gttgcgatcg tgcggggcgc
    17281 aaaagaatgc tttttgcaac gctggtggcg gttgccgctg gcgcacgaca ttatcaaaga
    17341 tgggcggatt gttgatgcgc agcagctggc taaaacgttg ttaccgtgga gtcgcgaact
    17401 gccgcagcgt catcacatta tgttggcgtt tcccgccagt cgcacattac agcggtcatt
    17461 tccgcgcccg tcgatgtccc ttggtgagcg ggagcaaacg gcttggctgt cagggacgat
    17521 ggcccgcgag ctggatatgg atccggactc cctgcgcttc gattacagcg aagactcact
    17581 cagccccgct tataacgtga ctgccgcgca aagcaaagag ctggcaacgc tgcttacgct
    17641 ggcagaaagg ttgcgtgttc atgttagtgc gatcaccccg gatgccagtg cattacagcg
    17701 attcctgcct tttttacctt ctcatcagca atgtctggcc tggcgtgata acgaacagtg
    17761 gctgtgggcg acgcgctata gctgggggcg caaactggcg gtggggatga ctagcgcgaa
    17821 ggagctggcg gcagcgttat ccgttgatcc cgacagcgtc gcgatatgtg gcgaaggcgg
    17881 atttgacccc tgggaggccg tttctgttcg tcagccgccg ctaccgccgc ctggtggaga
    17941 ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac tgatgaaccc gccaattaat
    18001 tttttgccct ggcgacagca acgccggacc gcttttctgc gtttctggtt gctgatgttc
    18061 gttgcgcctc tgctgctggc cgtcgggata acgctaatac tgcgtctgac aggcagcgcc
    18121 gaagcgcgca tagacgcagt tttgcttcag gcggaacaac aactcgcccg cagcttacag
    18181 ataacgaagc cacgtttgct ggagcaacag cagttacgcg aacagcgttc gcaacggcag
    18241 cgccagcgac aatttacccg cgactggcaa tctgcgctgg aagcactggc ggcgctttta
    18301 cccgagcacg cctggctgac aacgataagc tggcagcagg gaacgctgga gatcaagggg
    18361 ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt cactccgcca ggatgcgtct
    18421 tttcatctca atcagcgggg agccacgcag caggatgcgc agggacgctg gcaatttgag
    18481 tatcagttaa caaggaaggt tagcgatgaa catgttcttt gactggtggt tcgccacatc
    18541 accccgcctc cgccagtttt gctgggcatt ctggttgctg atgttagtta cgctcatttt
    18601 tctatcatcg acacaccatg aagagcgcga cgcattaatt cgactacggg caagtcatca
    18661 ccagcagtgg gccgcactgt atcgcctggt agacaccact cccttcagcg aggaaaaaac
    18721 gctgcccttt tcgccactgg attttcagtt atccggcgcg caactggttt cctggcatcc
    18781 atccgcgcag ggaggcgagt tggcgttgaa aacgctgtgg gaagcagtgc cgtcggcatt
    18841 tacacggctg gcagagcgca acgtcagcgt gagccgtttt tcgttaagcg tggaaggtga
    18901 tgatcttttg ttcacgctac aactggagac gccgcatgag ggttaaacgc tggttgttgg
    18961 caggtattgc attgtgcctt ttaaccggta tgcgtgaccc ttttaaaccg ccggaagatc
    19021 tatgccggat tagcgaactt agccagtggc gctatcaggg gatggtaggg cgaggcgagc
    19081 gcatcatcgg tgtaataaaa gacgggcaaa agaaatggcg acgggtgcag caaaactatg
    19141 tgctggaaaa cggctggaca attttacagc tgacgccaga cgtactaacg ctgggtaccg
    19201 ggacaaactg cgaaccgcca caatggttgt ggcaacggca aggagataca aatgaagcaa
    19261 tggatagccg cactactgtt gatgctgata cccggcgtac aggcggcaaa gccgcaaaaa
    19321 gtgacgctga tggtggatga cgttccggta gctcaggtgt tgcaggcgct ggctgaacag
    19381 gagaagttga acctggtcgt gtcgccagac gtcagcggta cggtgtcgtt acatctaaca
    19441 gatgttccct ggaagcaggc actacaaact gtagtgaaaa gcgccggact gataacgcgg
    19501 caggaaggca acattctctc agtgcattcc attgcctggc agaatgacaa tatcgcccgc
    19561 caagaggcgg agcaggcgcg ggcgcaggca aatctgccgc tggaaaatcg cagtataacc
    19621 ctgcaatacg ccgacgcggg agaactggcg aaagcggggg agaagctact gagtgccaaa
    19681 gggagtatga ccgtcgataa acgcaccaat cgccttttgc tacgagataa caaaacggcg
    19741 ttaagcgcgc ttgaacagtg ggtagcgcaa atggatctgc cggtcgggca ggttgagctg
    19801 tcggcgcata ttgtcaccat taatgaaaaa agtttgcgtg agttaggcgt gaaatggacg
    19861 ctggccgatg cgcaacacgc tggtggcgtt gggcaagtca ccacgcttgg tagcgacctc
    19921 tccgtagcga cggcgacaac gcatgtcggt tttaacattg ggcgcatcaa cggacgcttg
    19981 ctggatcttg agctttccgc gctcgaacaa aaacagcagc tggatattat cgccagtccg
    20041 cgtctgctgg cctcacatct tcagcctgcc agcattaaac aggggagcga aattccatat
    20101 caggtttcca gcggggaaag tggcgcgacg tcggtggaat ttaaagaggc cgtcctgggg
    20161 atggaggtca cgcccacggt gttacaaaaa ggtcgcatcc ggctgaaatt acacatcagc
    20221 cagaacgttc cggggcaggt gctacagcag gccgatggcg aagtgctggc gattgataag
    20281 caggagatcg aaacgcaggt cgaggtaaaa agcggagaaa cgttggcgct gggcggcatt
    20341 tttacccgta aaaataaatc gggtcagga
//
LOCUS       DADKXM010000065        20098 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.065, whole genome shotgun sequence.
ACCESSION   DADKXM010000065 DADKXM010000000
VERSION     DADKXM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.065"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..119
                     /locus_tag="JQR05_002324"
     CDS             <1..119
                     /locus_tag="JQR05_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324488.1"
                     /translation="VPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE"
     gene            520..1041
                     /gene="aroK"
                     /locus_tag="JQR05_002325"
     CDS             520..1041
                     /gene="aroK"
                     /locus_tag="JQR05_002325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY5324489.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            1098..2186
                     /gene="aroB"
                     /locus_tag="JQR05_002326"
     CDS             1098..2186
                     /gene="aroB"
                     /locus_tag="JQR05_002326"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY5324490.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            2278..3564
                     /gene="damX"
                     /locus_tag="JQR05_002327"
     CDS             2278..3564
                     /gene="damX"
                     /locus_tag="JQR05_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY5324491.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTELAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQVDLK"
     gene            3671..4507
                     /gene="dam"
                     /locus_tag="JQR05_002328"
     CDS             3671..4507
                     /gene="dam"
                     /locus_tag="JQR05_002328"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY5324492.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            4525..5202
                     /gene="rpe"
                     /locus_tag="JQR05_002329"
     CDS             4525..5202
                     /gene="rpe"
                     /locus_tag="JQR05_002329"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY5324493.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            5195..5953
                     /gene="gph"
                     /locus_tag="JQR05_002330"
     CDS             5195..5953
                     /gene="gph"
                     /locus_tag="JQR05_002330"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY5324494.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            5946..6950
                     /gene="trpS"
                     /locus_tag="JQR05_002331"
     CDS             5946..6950
                     /gene="trpS"
                     /locus_tag="JQR05_002331"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY5324495.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRMLKAVYEAIGFVAKP
                     "
     gene            7220..8125
                     /locus_tag="JQR05_002332"
     CDS             7220..8125
                     /locus_tag="JQR05_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324496.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            8135..8434
                     /locus_tag="JQR05_002333"
     CDS             8135..8434
                     /locus_tag="JQR05_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY5324497.1"
                     /translation="MMQVVNVLEKECHLPVRSEQGTMAMTHMASALMRSRRGEEIEPL
                     DNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEEGYLLANLYGLWMAANEEV"
     gene            8518..9681
                     /locus_tag="JQR05_002334"
     CDS             8518..9681
                     /locus_tag="JQR05_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY5324498.1"
                     /translation="MFVEELKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            9682..10908
                     /locus_tag="JQR05_002335"
     CDS             9682..10908
                     /locus_tag="JQR05_002335"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY5324499.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAECLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            10905..11783
                     /locus_tag="JQR05_002336"
     CDS             10905..11783
                     /locus_tag="JQR05_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY5324500.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            11794..12147
                     /locus_tag="JQR05_002337"
     CDS             11794..12147
                     /locus_tag="JQR05_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY5324501.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            12159..13463
                     /locus_tag="JQR05_002338"
     CDS             12159..13463
                     /locus_tag="JQR05_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324502.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            13475..14560
                     /locus_tag="JQR05_002339"
     CDS             13475..14560
                     /locus_tag="JQR05_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY5324503.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RATGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(14720..15451)
                     /gene="phoB"
                     /locus_tag="JQR05_002340"
     CDS             complement(14720..15451)
                     /gene="phoB"
                     /locus_tag="JQR05_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY5324504.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQTGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(15551..16336)
                     /gene="frlD"
                     /locus_tag="JQR05_002341"
     CDS             complement(15551..16336)
                     /gene="frlD"
                     /locus_tag="JQR05_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY5324505.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(16333..17163)
                     /gene="frlC"
                     /locus_tag="JQR05_002342"
     CDS             complement(16333..17163)
                     /gene="frlC"
                     /locus_tag="JQR05_002342"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY5324506.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(17213..18235)
                     /gene="frlB"
                     /locus_tag="JQR05_002343"
     CDS             complement(17213..18235)
                     /gene="frlB"
                     /locus_tag="JQR05_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY5324507.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(18256..19488)
                     /locus_tag="JQR05_002344"
                     /pseudo
     CDS             complement(18256..19488)
                     /locus_tag="JQR05_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(19779..19946)
                     /locus_tag="JQR05_002345"
     CDS             complement(19779..19946)
                     /locus_tag="JQR05_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY5324508.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         4757 a         4961 c         5581 g         4799 t
ORIGIN      
        1 gcgtaccgtt gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag
       61 aagatgaacg acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa
      121 cagccgtaaa agcggtaatg tttttatgct gaacgtgttt catctatttg acgcgcgcag
      181 gtatttagca tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca
      241 tgatgatgat ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg
      301 tgatttatcg ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga
      361 gggatgggtg atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg
      421 actctcgcaa tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg
      481 ggtttatcat taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc
      541 tttctggttg ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa
      601 ctcaatatgg aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg
      661 ggctgggttt tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc
      721 aatgagttga ccgagaaaca gggtattgtg ctggctactg gcggcggctc tgtgaaatcc
      781 cgtgaaacgc gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc
      841 gaaaagcaac ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca
      901 ccgccgcgtg aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt
      961 gccgacgtga ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt
     1021 cacatgctgg aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt
     1081 aaggtggatg tcgcgttatg gagaggattg tcgttactct cggggaacgt agttacccaa
     1141 ttaccatcgc atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg
     1201 agcaggtcat gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg
     1261 gcgtacttga acaggcgggt gttaacgtcg atagcgttat cctccctgac ggcgagcagt
     1321 ataaaagcct ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc
     1381 gcgatactac gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg
     1441 cggcgagtta tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg
     1501 tcgattcctc cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg
     1561 gcgcgttcta ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc
     1621 cgcgtgagtt agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg
     1681 cgttttttaa ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa
     1741 tggcgtactg tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc
     1801 gcgaaaccgg gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag
     1861 ctgaaatggg gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg
     1921 cggcgcggac gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa
     1981 ccctgctcac gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt
     2041 atttaccgca tatgctgcgt gacaagaaag tccttgcggg agagatgcgc ttaattcttc
     2101 cgttggcaat tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg
     2161 ccattgccga ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc
     2221 tattagcttc aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg
     2281 gatgaattca aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt
     2341 tctcgtcaat cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt
     2401 gatgatattg aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc
     2461 aatgaggaac cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa
     2521 gagcgcgtag agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt
     2581 cagtatatga tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt
     2641 tctgcgctaa aagccccctc gaccacttcc agcgatcaaa ccgcgtctgg cgagaagagt
     2701 attgatcttg ctggcaatgc gaccgatcag gcgaatggcg tgcagccagc gccgggaacc
     2761 acgtctgcgg aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact
     2821 caagggcaaa ccccggtggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg
     2881 aacaatgcgc tgacccagcc acaaaatcag caacagctga acaatgtggc ggtcaattcc
     2941 acgttgccga ctgaacccgc aacggttgcg cctgttcgca atggcaatgc atcgcgtgac
     3001 acggcgaaaa cgcaaaccgc tgaacgtccg tccactacgc gcccagttcg tcagcaggcg
     3061 gtgattgaac cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag
     3121 acaccgaagc gtactgaact agctgcccct gtggcgagca cgaaggcacc ggctgcgact
     3181 tctacgccag caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg
     3241 caaaccacgg caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa
     3301 tcggcaccgt ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg
     3361 aacggttggg cgaagaaaga gaatctgaaa aactacgttg tctatgaaac gacgcgtaat
     3421 ggtcagccgt ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa
     3481 gcggtatcta cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt
     3541 caggtacagg tcgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg
     3601 agctgcctga agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta
     3661 gttagtcagc atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct
     3721 gcttgatgat attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg
     3781 tgccgggtcg gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag
     3841 cgacctgatc agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc
     3901 acgcgagctg tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga
     3961 gttcaacaaa agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta
     4021 cggttacaac ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg
     4081 ctacaaaaaa ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa
     4141 tgcctttttc tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt
     4201 cgtctattgc gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca
     4261 cacaaacagt tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt
     4321 tgagcgccat attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta
     4381 tcagcgcgca aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac
     4441 acgtaaaaag gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa
     4501 aaaataattc tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg
     4561 gctgattttg cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc
     4621 gtgcattttg acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg
     4681 ctgaaatcct tgcgtaacta cggcattacc gcccctatcg acgtacatct gatggtgaaa
     4741 cccgtcgatc gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat
     4801 ccagaagcct ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa
     4861 gcgggtctgg tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag
     4921 ctggatgtga tcctgctgat gtccgtcaac cctggtttcg gcggtcagtc tttcattcct
     4981 caaacactgg ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt
     5041 cgtctggaag tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc
     5101 gcggatatgt tcgtcgccgg ttcggcaatc ttcgaccagc cagactataa aaaagtcatt
     5161 gatgaaatgc gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg
     5221 cgtcgctttt gatcttgatg gtacgctggt cgacagtgct cctggtcttg ctgctgcggt
     5281 agatatggcg ctgtatgcgc tggagttgcc cgtcgcaggt gaagaacgcg ttattacctg
     5341 gattggtaac ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg
     5401 tgcgactctg cgtaaaacaa tggggaaacc gcccgttgat gacgacattc cggcagaaga
     5461 acaggtacgt attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg
     5521 gacgtttttg ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc
     5581 gctaggcctg gtcaccaaca aaccgacgcc gttcgtcgcg ccgctgctcg aagccttaga
     5641 tattgccaaa tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca
     5701 tccggacccg ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt
     5761 tgtcggcgac tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg
     5821 cttaacctac ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta
     5881 tcagtctata aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc
     5941 gaaaaatgac taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg
     6001 gtaactacat gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt
     6061 actgtatcgt tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag
     6121 cgacgttgga tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca
     6181 tttttgttca gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgctata
     6241 cctacttcgg cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg
     6301 agaacatcaa cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt
     6361 atcaaactaa tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg
     6421 atatcgccca gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta
     6481 ttccgaaatc tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt
     6541 ctgacgataa ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga
     6601 aaatcaaacg cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga
     6661 acaaagcggg cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc
     6721 cagaactgga aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg
     6781 atgccgtttc cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg
     6841 aagccttcct gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc
     6901 gtatgctgaa agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tatcaaaacg
     6961 ctcggacggt cccctcgccc ctttggggag agggttaggg tgaggggagc aggcaagcgc
     7021 cagtgggaat taccctttgc tcgcattgtc gaataaatca cttcgtcatc actctgaaac
     7081 cggggaaacc cggttttttt atcctctttt cgcaacaacc cgaaaaaatg tgaagcgcct
     7141 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattt
     7201 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
     7261 gacactggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
     7321 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgctgga
     7381 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc ttgtcgagat
     7441 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
     7501 gccctatacc cgcttgtacg aaggtttagc gagcggattg aaagcccagt ttgatggcat
     7561 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
     7621 tgtctatgac atggcagtag tttcccgcct ggcggcggaa agttatctca gccaaaacaa
     7681 tttgtgcatc gccctcgagc ttggcccgca tacgtatgtt ggtgaacatc aactgatatg
     7741 ccgcaaaggt gagtctggaa atgttaagcg agtaggtctc gatagtcgtt cggcggatca
     7801 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgagtcg atctctctta
     7861 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggtgatct ggaacgtggt
     7921 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctaacag acgatccgcg
     7981 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
     8041 attgctgcgt gccgttgtag ataaacacgc cctgcttgcc catcaacaac gggtggtgag
     8101 tggggaacag gaacccagtt attaaacgaa aggcatgatg caggtcgtca acgtactgga
     8161 aaaagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga cacatatggc
     8221 gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata acgagttgct
     8281 ggcggaactg gcgcaatcca gccactggca agccgttgtg caattgcatc aggtgttgtt
     8341 gaaggaattc gctctggaag ttaacccgtg tgaagaaggc tatttgctgg ccaatcttta
     8401 tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc acgtcgcaca
     8461 acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg caacaagatg
     8521 tttgtagaag aattgaaacg ccagaacccg gcgctgattt ccgccgcact aagtttgtgg
     8581 cagcagggca aaatcgcccc ggacagctgg gtgatcgacg tggatcagat actggaaaac
     8641 ggtaagcggc tcattgagac ggcgcggctt tacggcattg aactgtatct gatgaccaag
     8701 caatttggtc gtaatccgtg gctggcggaa aaattgctgg cattaggcta cagcggcatt
     8761 gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgcc agtggcgcat
     8821 caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga acagggcacc
     8881 gacatcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc agcggttaag
     8941 gccggacggg gccagcctgt gctgctcaaa gtttatagcg acgatgattt tctttatccg
     9001 gggcaggaga gcggttttgt acagcattcg ctgcacgagg tgattgctga aattaagaaa
     9061 ctgccggggc tgcatttagc cggactaacc catttccctt gcctgctttg ggatgaggct
     9121 gccgggaaag ttttgccaac accgaatctt cacacgctgg tacaagcacg ggatcaactg
     9181 gcgaaatctg gtattgcgat tgagcaactg aacgtacctt cagcgaccag ctgcacttcg
     9241 ctgccattgc tggcggaata cggtgcgact catgccgaac ccggtcatgc gctgaccggt
     9301 actattccgg caaaccagca gggcgatcag cctgaacgta tcgcgatgct ctggttaagt
     9361 gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta ctaccgtcgt
     9421 ggtcatgcgc aacatgcgct ggtgtttacg ccagaaaatc aaaagattac tgaaaccaat
     9481 ctcaaaactg tggatgacag cagtatcgac tacaccctgc cgctggcagg cgagtatccg
     9541 gtgagcagtg cagtggtgct ctgttttcgc acgcagattt ttgtcacccg tagcgatgtg
     9601 gtgctggtgt ccggtattca gcgcggcgaa ccagaaatag ttggtcgtta tgacagcctc
     9661 ggcaaccctc tggaggcata aatggcgcga tttgtggtgt tagtgattga tagctttggc
     9721 gtaggggcaa tgaaagatgt cacgctggtg cgcccgcaag atgccggggc gaatacctgt
     9781 ggtcacatcc tgagccagtt gccgcatttg cagctaccaa cgctggagaa gctggggcta
     9841 atcaacacat tgggttatgc gccaggcgat atgcagccgt cagattccgc aacctggggc
     9901 gtggcagagc tgcaacatga aggtggcgat acctttatgg ggcatcagga aattttaggc
     9961 acgcgcccgt taccgccgct gcggatgcct tttcgcgatg tgattgaccg tgttgagcag
    10021 gcattagttt ccgctggctg gcaggtggag cgccgtggcg atgatctgca atttctgtgg
    10081 gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg atttaggcca agtctataac
    10141 attaccgcca atctctctgt gatctctttt gacgacgcaa tcaaaattgg tcgtatcgtg
    10201 cgtgaccagg tacaggtcgg tcgggtcatt acatttggcg gcctgttaac caacagtcag
    10261 cgtattctcg atgccgcaga aagcaaagaa gggcgcttta ttggtattaa tgcgccgcgt
    10321 tctggcgctt atgacaacgg cttccaggtc gtgcatatgg gctacggtgt tgatgaaaaa
    10381 gtgcaggtgc cgcaaaaact gtatgaagcg ggcgtgccaa ccgtgctggt gggtaaggtg
    10441 gcagatatcg tcagcaatcc ttatggtgtg agctggcaaa acctggtgga tagccagcgg
    10501 attatggata tcaccctcga cgaatttaac acccatccga cggcgtttat ttgcatcaat
    10561 atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg cacgttatgc cgaatgtttg
    10621 caggtcgttg accgtaacct tgcccggctt gttgaggcga tgcagccaga tgattgcctg
    10681 gtcgtgatgg cggatcacgg taacgatccg accattggtc acagccacca tacccgtgaa
    10741 gtggtgccag tgctggttta tcagcaaggg ctggtcgcca cgcagctcgg tgtgcgcacc
    10801 acgctttctg atgtgggagc taccgtgtgt gaatttttcc gtgcgccacc gccacaaaat
    10861 ggtcgctctt ttctttcctc cctccggttt gcaggagaca ccctatgagt tttgatccga
    10921 cgggttacac cctggcccat gagcatctgc atattgatct ctccggcttt aaaaacaacg
    10981 tggactgccg ccttgatcag tatgcgttca tttgccagga gatgaacgac ctgatgaccc
    11041 ggggtgtacg taatgtgatt gagatgacca accgttacat ggggcgcaat gcgcaattta
    11101 tgctcgatgt aatgcgcgaa accgggatca acgtggtggc ctgtactggt tattaccagg
    11161 atgcgttttt cccggaacat gtggtgaccc gcagcgtgca ggaactggcg caggagatgg
    11221 tcgatgaaat tgaacagggt atcgatggca cggagctgaa agccgggatc atcgcggaga
    11281 tcggctccag cgaaggaaag attacgccgc tggaagagaa ggtatttatt gccgctgcgc
    11341 tggcgcataa ccagaccgga cgcccgatct ccacgcatac gtcgttcagc accatggggc
    11401 tggaacaact ggctctgttg caagcccacg gagttgatct ttcgcgcgtc accgttggtc
    11461 actgcgatct gaaagacaac ctcgacaaca ttttgaagat gatcgatctc ggcgcgtacg
    11521 tgcagttcga caccattggt aagaacagtt actacccgga cgaaaagcgc attgcgatgc
    11581 tccacgcgct acgtgaccgt gggttgctga accgcgtcat gctgtcgatg gatattacgc
    11641 gccgctccca tttaaaagcc aatggtggtt atggctatga ctatttattg accaccttta
    11701 ttccgcaatt gcgccagtca ggattcagtc aggccgatgt ggatgtgatg ttacgtgaaa
    11761 atccctctca atttttccaa taaggacaga ctcatgaaaa agattggcgt tgcaggctta
    11821 cagcgtgagc agattaaaaa aaccattgaa gcgacggctc ctggctgttt tgaagttttc
    11881 attcacaacg acatggaagc ggcaatgaag gtgaaatccg ggcaactaga ttattacatt
    11941 ggcgcgtgta ataccggtgc gggcgcggca ttgtcgattg ccatcgcggt gattggctat
    12001 aacaaaagtt gcaccattgc caaaccaggc attaaagcga aagacgagca tatcgccaaa
    12061 atgatcgctg aagggaaagt ggcgtttggc ctttccgttg agcacgtcga acacgcgatt
    12121 ccgatgttga ttaaccatct gaaataaaag gcacgactat ggatctgtat attcagatta
    12181 tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc gcatcgctcg gcggctgttt
    12241 ttcatgacgg catccacccg atcctgccgc aactgattga aggctatatg aaccgtcgcg
    12301 aggcggggag tatcgctttt ggtctgagca ttggttttgt ggcctcggtg gggatctctt
    12361 ttaccctgaa aaccgggctg ctcaacgcat ggttactctt tcttcctacc gatatcctcg
    12421 gcgtactggc gataaacagc ctgatggcgt ttggtcttgg cgctatctgg ggcgtgttga
    12481 tccttacttg cctgttgcca gttaaccagc tgctgaccgc gctgccggtg gatgtattag
    12541 gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt tgcactgttc ccgctggtgg
    12601 cgattttcta ccagtttggc tggaagcaaa gtctggtcgc cgccgtggtt gttctgatga
    12661 cccgtgtggt agtcgtgcgc tatttcccac atcttaaccc tgaatccatc gaaatcttta
    12721 ttggcatggt gatgctgctg gggatcgcga taactcacga cctgcgtcat cgtgatgaaa
    12781 atgacatcga tgccagcggg ctttcggtgt ttgaagagcg cacgtcgcgg attatcaaaa
    12841 acttacctta tatcgccatc gtgggagcat tgattgccgc cgttgccagt atgaagattt
    12901 ttgctggtag cgaagtttcg atcttcacac tggagaaagc gtattccgca ggcgtgacgc
    12961 cggaacaatc gcaaacgctg attaatcagg cggctctggc agaatttatg cgcggactgg
    13021 ggtttgtgcc gttgattgcc accaccgcct tagcaacggg tgtgtatgca gttgcgggct
    13081 ttacctttgt ttatgcggtg ggctatctct cgccgaatcc gatggttgca gcggtattag
    13141 gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc gatcggcaaa tggctgggac
    13201 gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa cgccatgaat atgctgatgg
    13261 aagtggcgct gctggtcggt tcgattttcg cagcaattaa gatggcgggt tataccggat
    13321 tctctatcgc ggttgccatt tacttcctca acgaatccct gggccgtccg gtacagaaaa
    13381 tggcggcacc ggtcgtggca gtaatgatca ccggtattct gctgaatgtt ctttactggc
    13441 ttggcctgtt cgttccggct taaggaaggc acctatgaag acgtttcctt tgcaaagcct
    13501 gactcttgct gaagcgcagc aaaagcagtt tgcgctggtg gacactattt gccgccattt
    13561 tccaggcagc gagtttcttg ctggcggtga tttagggtta actccaggtc tgaatcaacc
    13621 gcgtattacc caacgggtgg agcaggtgct ggctgatgca tttcacgcac aggccgcggc
    13681 gctggtgcag ggcgcgggga ctggcgcgat tcgcgccggg ctggcggctt tgctcaaacc
    13741 ggggcagcgt cttctggtgc atgacgcgcc tgtttacccg acgacacggg ttattattga
    13801 gcagatgggg ctgacgctta ttactgctga tttcaatgac ctgtcggcac tgaagcaggt
    13861 cgtcgacgag caacaacctg atgccgcgct ggtgcagcat acgcgccagc agccgcagga
    13921 cagctatgtg ctggcagatg tgctggcaac gttgcgcgcg acaggtgttc cagcgttaac
    13981 cgatgataac tatgcggtga tgaaggtagc gcgaatcggc tgtgaatgcg gcgcgaatgt
    14041 ctcgacattc tcctgcttca agctgtttgg gccggagggc gttggtgcag tggttggcga
    14101 tgccgatgtt attagtcgga ttcgcgccac gctttactcc ggcggtagcc agatccaggg
    14161 ctcacaggcg ctggaagtct tgcgtggtct ggtgttcgcg ccagtgatgc atgcggtgca
    14221 ggcgggagta tccgaacggt tgctggcttt gcttaacggc ggcgcggtgg cggaagtgaa
    14281 aagcgccgtc attgccaatg cgcagtcgaa ggtgttgatt gtcgagtttc atcagctgat
    14341 tgccgccagg gtgctggaag aagcgcaaaa gcgtggcgcg ttgccttacc cggtcggtgc
    14401 agagtcgaaa tatgaaatcc cgccgctctt ctatcgactt tcaggaacgt ttcgccaggc
    14461 gaatccacaa ctcgaacatt gtgcgattcg cattaacccg aatcgcagcg gtgaagagac
    14521 ggtgctgcgg attttgcgtg agagtattgc gagtatttaa tgctggatag caccgttcgg
    14581 caatcaagct aaattccgca ctgaacggtc ccctcgcccc tttggggaga gggttagggt
    14641 gaggggaacg tgcgggatgt aaaattcagt gcgccagcat cgccggatgc gatgctggcg
    14701 catcttatcc ggcctacggt taatgtcttt tattatcaat cgttaaggtg atgcgattgg
    14761 cgcggcagaa gagttcggag acgtgcaccg gtttgtcatt ctgatcaaag gcgattttgc
    14821 tgatgcgaaa caacggttcg cccagttcgc atttcagcca ttttgcctgc gggcgggtgg
    14881 cggcaaagat atcgatggtc tttttgtcgc tgaccactcg cgtgtcaaaa cgctcctgaa
    14941 ataactgata ggtggagctt ccttcgacgt aaatctcatc aaagtcagga taacgcgaca
    15001 gcgggatcca tgaactatca ataaacagcg gctctttatc gagatacatc acccggcaga
    15061 gatggaacac ttcgctgttt ccggggatgt tcagcttttc gcaaaacggc gcggcgctga
    15121 cccgttcctg ttcgatcact ttctctttcg tcgccttgcc ttgtgagacg ccaaaatcgg
    15181 taaaaccact gacagtaagc agggcgtttt caactttctg gctttgtaca aaggtgcctt
    15241 ttccctgcca gcggatcagt acgccgtctg ccactaagtc gctgatggct ttgcgaatgg
    15301 taatgcggct gacgttatat tgtgtacaaa gctcgttttc ggtagggatc tgttgcccgg
    15361 tctggtaaac cccctgcgcg atatcatcca gcagccgctg gcggacggta gcgtagagga
    15421 gttgatgaga gtagcggtcc gtagctgaca taatcgaccc tgaaataaag aatgaattac
    15481 tcggattata gggtcgtttt ccggcaagta acagcacgtt ggcgtgaaga tgctcacgcc
    15541 aacgttatac ctaccaggca ccgtggtact gaatggtttt cgccgcgcac gccgttccct
    15601 gcgccatcgc ctgcggtaat gtcatccccg cagaccagcc gcaaaggaat ccggcaatga
    15661 acgaatctcc ggcacccatg gtgtcgataa ccgtcaccgg ttcaggagcc tgacgccaga
    15721 actgcgcgcc atcccaggca atgctgccgt tttcacccag cgtgacaatc actgttcctg
    15781 cgccacgggc aacaatcgct ttcatcttca gacgcagcgc ttcgtcttct tgcggtgcgg
    15841 aggcaaaggc aaaatcgaga tgcggcacca gtgtctgcca gagcgggctg tcccacttgt
    15901 cggagaagtc gaaagcggta agtttgcccg cagcgtgcag ctgtgggaat gcgtcttccg
    15961 catgtcccca gattgccgcg tgcacaatgt catactgcgc cagccaggcg taatcctctt
    16021 cactcagggc aaagtcggcc atcacgcctt cggtgtagtc gccaaaaacg cgatcattgt
    16081 cgtgcagttc cacctgagtt tgtgcggtaa cgccgtgttt cgtatggaca tggctgatat
    16141 cgacgcccat gcgggcgaga tcctgcttca gctttgtgcc gtagtcatcg tcacccaccc
    16201 aggtaatgca tcccggctgt atgccgtagc gagtgcagta caccgccaca ttgaccgcat
    16261 taccgccaga aaacgcttta ttcagttgcg ggtagatatc gacgcagtta tcgccgattg
    16321 tcgccagggt tttcatctct catcctccgg cagcagcgcg cgaaagcgtt ccagcgcctg
    16381 gcgggcatag agtctgggct cgttcatata catcgtcacc agctccaccg tacagtaacc
    16441 ctcatagccc cgatcaataa tatcgcgcat cagttcccgc agcggcattt tgccttctcc
    16501 aggaatgtaa tgcgtgtcgc tggccccgtc gctgtcgaca atatgcagat gacgtaattt
    16561 atcgcccagt ttgtcgaaat aactcatcac cggttccgcc tggacatacg gcgcgcaaat
    16621 gtcgaccatg ctgaacaggc gcggcgaagg caccagcgcc agcgcatgaa gcacatcatt
    16681 agcattacac acgacgttcg attcatacgg cgttaacggt tcgaggatca gatccatgcc
    16741 gatgttttcc gcgtactcac acagttcgct taagttctct gccagccgcc cccagataac
    16801 gttaggcggc gtgagatagc ccgcgtgggc cgcagaaatc agcgtataac ccgcgttcat
    16861 ctcttttgcc atatctatcg ccagcttgat catgtccagg ctttcacgac gcatatgttc
    16921 atcgcccagc atcatgttat acggatagcc gttggtttct ggcgtgtaac cgataatcgg
    16981 catctgatac gtctgcgcca gcgccttgat ttgtttgatg ccgcccgctt ttaagtccgg
    17041 cgcgaacgcg tgcgggcgac cgccccaaat ttcgatgccg tcgtaaccca gctcgcttgc
    17101 atcacgaaat gcgtgttcaa taggcagccg ctggtggccg caggtaaaca tacctgtttt
    17161 catcgggtaa ctccttgcgt aaatccggcc cgttgccggg ccgggagagg gattaatatt
    17221 ccaccagacc accgtaatag cggcgttcat ccgggttgtg atctttgtaa atagacaggt
    17281 agtagcagag ccactccatt ggcacgaaca tcaggaacgg tgccagccac gggtgcagcc
    17341 cttgcgaaat ttcggcgtaa tcgatgacga tcacgttgtc agtacgctgt ttaacaaagt
    17401 taatggcgcg ttcggtggtg tggcgacttt catcattgcc gagcaggaac aggaacggaa
    17461 cgcccggttc gacaatctcc agcgggccat ggcggaactc tccgctctca atcacgcagc
    17521 cgtgcgtcca ggtaaattcc atcagcgtta caatgccttc tttgtaaccc agcggacgca
    17581 gcggacccgc agcaacggta taaatcatcg gccactggct ggccagttca ccaagctggc
    17641 ggcctttttc ttcccaggtg cgtaccagat gaccgagcgc attcggcaac tgcttgagat
    17701 cgtttttgat cttgccgatt tccgcgttcg gcgcgaggcg ggtgatcatc tccagcacca
    17761 cgctgtagca gagcagcagg tgaatttccc agatacagtc ggcctgataa tcaatgctaa
    17821 attccgccgc cgaggtaatc gggctgtccg cgcgtttggt gaacgccgca gtgagtgcgc
    17881 cgcaggcccg gcccagctcc agcgctttga ttacctcttc cgttttaccg tagtcagaaa
    17941 cgccaatcac cgcgcaacga tcgtcgaggc gatacggggt gttatcgcag aactcccagc
    18001 cggaaatggc gtagacctga agatcggaaa agcgatccgc cagatgtttc gccgtttgcg
    18061 ccgcgttgag tggcgagccg caggcaacga aataaatacg atcaatgtcg cgcttcacca
    18121 tctcttccac gatggcgtgc accagcggaa cgtcatggct gagaactttt tccacttcct
    18181 gaaccatatt ttcggtgacc agaaagtcca cggtgctttt atcaatatcc aacattttat
    18241 tttcttcaga taacattacg acaaagcgtt gagctggcgg ctacgcttcg cccagaaggc
    18301 gtaagcaggc agaccagtag caataacgat gacggcgcag ataaggccgg gaatcggtgc
    18361 ccagacaaag gttgaggcga ccagaatgag gcttgacgca atggcgaggg tggtcatcag
    18421 cccgaaagcc ggagtacgcc acagcggttt gtaatcgtcg cgtttacgac accagatgat
    18481 ggagccgaag gtgagggtat ttttgaaaca catcaccagg gtgaaataac ccagcaggct
    18541 ggtgagatcg gaaacgaaga tgaagaagat ccccagcgcc ccttgcagga tgatggagac
    18601 atccggcgtg ttgtatttcg gatgcacatg gccgaagcat ttaaagaaca ggttgtcttt
    18661 cgccatcgcg tattccagcc gcggctggta catcacgcag ctggaaagcg aaccaagaat
    18721 gacgatcatc gccgtgatgg caacaaagat cccagcggtg ctgccgagtg cggggatcta
    18781 ggtcagggcg tcggaaatcg gcgtttcaga attggcgagt ttgtcgaagg gcatcaggcc
    18841 ggaaatcacc agcgccagca gggtgtagag caccagaacc agcagacagg aaccaatcag
    18901 cgccataaag ctgcccgttg cgccaatcgc agtggtggta agggcggcaa aattctccgc
    18961 tttaaaccag aagatcccaa ggccaatgac gatagtgaac gggataattt tggcgatggt
    19021 aattagcgtc tgaaacgctg cgccgccttc aacggagcgc aggtgtagca acataaaggc
    19081 gataattaat ccggcggcga taaatttacc gagcaacgga tcgataggcg ttaaaaagcc
    19141 aagattgctg acaatcgcca gcgccataat cgacaatgac ggcgcatcgt tggcccagaa
    19201 gctggcccag ccggagagga aagccagcgg tcggcttccg gcatttttca gataaacata
    19261 atctgcgcca ttttccggat aagcggtgga tagttccgca tagacgcaca tttgcgggat
    19321 cacaattaac ccgccaatga caaacgcgag caccgtaagc cacggcgtgc ccgctgcttt
    19381 tgccacttca cccacagata caaaaatacc ggagccgacg gttgtcccga cggcgattgc
    19441 aagaacggcc caaaatccga gcttgcgttg gagttcctgg cttcccatag cgatttttct
    19501 tgtccaatac gttgtagggg agagtttgca gtcatttaaa aggtaacatg acaatgcatg
    19561 atgcatataa caacatatga tgttatgtaa tgtggaggaa gatcacaaaa tttcgcacag
    19621 gatcgcgctg tggctaatgg atgtagttat caaattgaat ttaaagtgaa aatattttta
    19681 cgggcggggg caagaaggac atagaaacaa atacgccctc ggaaaatcca gagggcgtcg
    19741 ggcaattaaa ccggtgttag ccgatttctg tcagagactt actgtgcagt aggaccgcaa
    19801 ccgccaatga ttttagaaat agaaattgcc gggtgcagca ggtagtcgta agagcagttc
    19861 ttatcacggt tttcaatatg accagtacat gcagttaacg tagccagtac tgcacctacc
    19921 agtgcaactt taataaattt gttcatttac attccctatg tattatatat taattaatat
    19981 tcattgatta tatgaatata agcagactgc tcgttaagaa cgatacagga atatttaatt
    20041 ggcgtcagtg agcgaatgac ttaattttgt aagtcgctgt aacggtgaat ataaccca
//
LOCUS       DADKXM010000066        19841 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.066, whole genome shotgun sequence.
ACCESSION   DADKXM010000066 DADKXM010000000
VERSION     DADKXM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.066"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..441
                     /locus_tag="JQR05_002346"
     CDS             <1..441
                     /locus_tag="JQR05_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY5324509.1"
                     /translation="DHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGL
                     AVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQRVC
                     ASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JQR05_002347"
     CDS             complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JQR05_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY5324510.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JQR05_002348"
     CDS             complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JQR05_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY5324511.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3666..4280
                     /gene="evgA"
                     /locus_tag="JQR05_002349"
     CDS             3666..4280
                     /gene="evgA"
                     /locus_tag="JQR05_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY5324512.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4285..7878
                     /gene="evgS"
                     /locus_tag="JQR05_002350"
     CDS             4285..7878
                     /gene="evgS"
                     /locus_tag="JQR05_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY5324513.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNNYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPSVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFKIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JQR05_002351"
     CDS             complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JQR05_002351"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY5324514.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JQR05_002352"
     CDS             complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JQR05_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY5324515.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLEPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JQR05_002353"
     CDS             complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JQR05_002353"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY5324516.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JQR05_002354"
     CDS             complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JQR05_002354"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY5324517.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(13678..14301)
                     /locus_tag="JQR05_002355"
     CDS             complement(13678..14301)
                     /locus_tag="JQR05_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY5324518.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            14598..14873
                     /locus_tag="JQR05_002356"
     CDS             14598..14873
                     /locus_tag="JQR05_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324519.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(14950..15192)
                     /locus_tag="JQR05_002357"
     CDS             complement(14950..15192)
                     /locus_tag="JQR05_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY5324520.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            15545..16465
                     /gene="lpxP"
                     /locus_tag="JQR05_002358"
     CDS             15545..16465
                     /gene="lpxP"
                     /locus_tag="JQR05_002358"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY5324521.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTIVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16820..16891
                     /gene="ypdK"
                     /locus_tag="JQR05_002359"
     CDS             16820..16891
                     /gene="ypdK"
                     /locus_tag="JQR05_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY5324522.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JQR05_002360"
     CDS             complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JQR05_002360"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY5324523.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            18571..>19841
                     /locus_tag="JQR05_002361"
     CDS             18571..>19841
                     /locus_tag="JQR05_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY5324524.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQI"
BASE COUNT         5629 a         4593 c         4220 g         5399 t
ORIGIN      
        1 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
       61 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
      121 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
      181 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
      241 caggaagaag gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc
      301 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca attacgtaat
      361 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
      421 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
      481 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
      541 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
      601 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
      661 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
      721 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
      781 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
      841 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
      901 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
      961 ccctggaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     1021 gaacgccagt aatagcaaac cgcatacatc aaaaaactga atgtcactaa cacccgcatg
     1081 tctattttat tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg
     1141 ggcgcataag caagcccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     1201 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca
     1261 atggtgaaat tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     1321 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     1381 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     1441 ccgagcacta acagggtcag tcctggtaga ttcattttta ccggtgaagt ttcagtttct
     1501 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     1561 aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat
     1621 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     1681 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc
     1741 tgaacgactc taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa
     1801 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     1861 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
     1921 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     1981 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat
     2041 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     2101 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     2161 tactggtata agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt
     2221 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     2281 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     2341 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     2401 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     2461 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     2521 gacgtgccgg tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa
     2581 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca
     2641 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     2701 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     2761 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     2821 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
     2881 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     2941 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     3001 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     3061 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     3121 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     3181 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     3241 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     3301 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     3361 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     3421 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     3481 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     3541 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     3601 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     3661 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     3721 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     3781 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     3841 acggtatcca ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     3901 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     3961 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     4021 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     4081 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     4141 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata
     4201 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc
     4261 aacgtaacaa aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt
     4321 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     4381 agtaacaacc gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta
     4441 gcgagcaaaa aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat
     4501 accgattcgc agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     4561 gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc aatggacgcg
     4621 cttgcagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat
     4681 aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt
     4741 cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat
     4801 taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     4861 aatttatatc aggcattagc atccgtctca gctgggcaca ataattactt tattggtagt
     4921 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     4981 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
     5041 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
     5101 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     5161 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
     5221 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
     5281 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     5341 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
     5401 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     5461 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     5521 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
     5581 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
     5641 gtcgcgacac agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
     5701 cattttctta ttcctagcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     5761 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
     5821 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
     5881 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     5941 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     6001 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     6061 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     6121 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     6181 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     6241 tttaaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     6301 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     6361 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     6421 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     6481 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     6541 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     6601 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     6661 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc
     6721 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
     6781 ttatcaaatt tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     6841 acctccctgg gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     6901 ggaagtggat tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt
     6961 gcaggtcgtc agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa
     7021 aatatgcagg gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc
     7081 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc
     7141 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     7201 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     7261 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     7321 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     7381 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     7441 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     7501 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     7561 aataatacgg cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     7621 acgcataaag atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc
     7681 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     7741 aatattagcc atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt
     7801 cagctgctga actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc
     7861 tgtcagaaaa atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca
     7921 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     7981 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     8041 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
     8101 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
     8161 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
     8221 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     8281 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     8341 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
     8401 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     8461 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     8521 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
     8581 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
     8641 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     8701 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
     8761 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     8821 atatactttt atcatgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     8881 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     8941 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
     9001 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     9061 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     9121 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     9181 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     9241 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     9301 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
     9361 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
     9421 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     9481 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
     9541 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     9601 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     9661 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     9721 gatacggaat caccataaat aggctcgaga actgcaaacc ccaggaatcc aatggtaggt
     9781 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     9841 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     9901 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     9961 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    10021 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    10081 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    10141 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    10201 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
    10261 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    10321 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    10381 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    10441 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    10501 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    10561 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    10621 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    10681 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    10741 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    10801 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    10861 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    10921 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    10981 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    11041 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    11101 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    11161 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    11221 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    11281 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    11341 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    11401 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    11461 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    11521 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    11581 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    11641 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    11701 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    11761 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    11821 ataatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    11881 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    11941 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    12001 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    12061 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    12121 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    12181 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    12241 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    12301 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    12361 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    12421 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    12481 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    12541 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    12601 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    12661 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    12721 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    12781 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    12841 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    12901 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    12961 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    13021 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    13081 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    13141 taattccttg aagtggagtt gacatagcca tacctattag ttaattaaat atgtgttaat
    13201 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtggcg
    13261 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    13321 attcctttct gcatggtaag tagtgatgac gaaaagaaga atatgataaa tattgattcg
    13381 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    13441 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    13501 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    13561 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    13621 aaaaattgtc ggctggagtg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    13681 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    13741 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    13801 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    13861 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    13921 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    13981 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    14041 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    14101 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    14161 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    14221 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    14281 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    14341 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    14401 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    14461 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    14521 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    14581 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    14641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    14701 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    14761 cacaggcgat tttgtttaag atgagagaag atgttccacc attaaacctc acagaagaaa
    14821 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    14881 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    14941 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    15001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    15061 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    15121 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    15181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    15241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    15301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    15361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    15421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    15481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    15541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    15601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    15661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    15721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    15781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    15841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    15901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    15961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    16021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    16081 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    16141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    16201 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    16261 aacgatagta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    16321 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    16381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    16441 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    16501 cataataacc tcatgatttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    16561 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    16621 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    16681 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    16741 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    16801 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggttatcgt
    16861 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    16921 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    16981 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    17041 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    17101 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    17161 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    17221 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    17281 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    17341 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    17401 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    17461 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    17521 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    17581 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    17641 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    17701 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    17761 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    17821 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    17881 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    17941 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    18001 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    18061 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    18121 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    18181 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    18241 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    18301 aacattttta tcaatggctt taaatgagga actccacgtc aaaaacagga aatttttcta
    18361 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    18421 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    18481 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    18541 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    18601 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    18661 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    18721 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    18781 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    18841 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    18901 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    18961 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    19021 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    19081 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    19141 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    19201 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    19261 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    19321 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    19381 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    19441 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    19501 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    19561 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    19621 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    19681 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    19741 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    19801 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta a
//
LOCUS       DADKXM010000067        19697 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.067, whole genome shotgun sequence.
ACCESSION   DADKXM010000067 DADKXM010000000
VERSION     DADKXM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.067"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JQR05_002362"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JQR05_002362"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY5324525.1"
                     /translation="MKGRRVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1542..2348
                     /gene="tcdA"
                     /locus_tag="JQR05_002363"
     CDS             1542..2348
                     /gene="tcdA"
                     /locus_tag="JQR05_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY5324526.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JQR05_002364"
     CDS             complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JQR05_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY5324527.1"
                     /translation="MTNPQFAVHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JQR05_002365"
     CDS             complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JQR05_002365"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY5324528.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4239..4466
                     /locus_tag="JQR05_002366"
     CDS             4239..4466
                     /locus_tag="JQR05_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY5324529.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4817..5734
                     /locus_tag="JQR05_002367"
     CDS             4817..5734
                     /locus_tag="JQR05_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY5324530.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5753..6148
                     /locus_tag="JQR05_002368"
     CDS             5753..6148
                     /locus_tag="JQR05_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY5324531.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6141..7241
                     /gene="rlmM"
                     /locus_tag="JQR05_002369"
     CDS             6141..7241
                     /gene="rlmM"
                     /locus_tag="JQR05_002369"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY5324532.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JQR05_002370"
     CDS             complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JQR05_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY5324533.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JQR05_002371"
     CDS             complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JQR05_002371"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY5324534.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JQR05_002372"
     CDS             complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JQR05_002372"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY5324535.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVNKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTSELDSQAWLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     YELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JQR05_002373"
                     /pseudo
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JQR05_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JQR05_002374"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JQR05_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY5324536.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JQR05_002375"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JQR05_002375"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY5324537.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JQR05_002376"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JQR05_002376"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY5324538.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JQR05_002377"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JQR05_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY5324539.1"
                     /translation="MRGLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JQR05_002378"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JQR05_002378"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY5324540.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JQR05_002379"
     CDS             complement(17863..19152)
                     /locus_tag="JQR05_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY5324541.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4870 a         4824 c         5194 g         4809 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgtagg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcacat cctgattatt gactaagtgc
     1381 gcgttgttca taccggatgc ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc
     1441 aaaatattgc aggagttatg taggcctgat aagcgtagcg catcaggcat ttattcgttt
     1501 agggtgaggg ataacctcgc cctttttaat tctgaggtgc tatgtctgtg gtaattagtg
     1561 atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac
     1621 tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg
     1681 aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat gatgtgtgcg
     1741 tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg
     1801 aagtcatggc ggagcgcatt cgccagatta acccggagtg ccgtgtaacg gtggtggatg
     1861 atttcgtgac gccggataac gtagcgcaat atatgagtgc cggttattcg tacgtgattg
     1921 atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa
     1981 tcccgctggt tacgaccggt ggcgcgggtg ggcagattga cccgacgcag attcaggtta
     2041 ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa
     2101 gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtggattgc gtgttttcta
     2161 ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag
     2221 aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca
     2281 cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc
     2341 agggttaaac aatcaggtcg gatgagacgc gaacgtctta tccgacccgg ttctgtgctt
     2401 aaacctgctt cgtcgcagcg ataatcgcct cgcttaacgc atttaacccc tggctgcgtg
     2461 aggcgctaag ctgcgcacgt aatcccagct catcaaacaa tgccagcggc gactgtgcct
     2521 gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac aggccgcgca
     2581 caatgcgccc ttcgctgtcg ccaaagaagt gcattttgcc gttttcagcc acggtatatc
     2641 ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt aactcgtctg
     2701 gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct tcccattgcg
     2761 acaacggtgc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg aacggatgta
     2821 cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt caacggcatt
     2881 caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc
     2941 ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat
     3001 accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa aatcaaaggc
     3061 cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg ccagcgcttc
     3121 ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat cgtaatctgc
     3181 cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg ttcctgcttc
     3241 cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt
     3301 gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat acagcacgcc
     3361 gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga tatccagttg
     3421 ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca tcaaccatca ccaccatccc
     3481 ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg caaccgccag tcacgttcga
     3541 catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca acaaatcgac
     3601 atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctctag tttgttgggc
     3661 gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa taatctcatc
     3721 gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg attcagtggt
     3781 gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg ccactttctc
     3841 tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac
     3901 gtttccggcg ctcaggctgt aaaactgtcg agtggcttca accacggctt caggtttaag
     3961 cgcggtcgcg gcgctatcga gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg
     4021 aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc gatcgtgacg
     4081 caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctgga tatcctggcg
     4141 aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc tgttaatcaa
     4201 tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact gccgcaatta
     4261 tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac tatgtgatgc
     4321 acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat aacgataccg
     4381 gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt actgacgtga
     4441 aagagatggt cgaactggaa cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat
     4501 taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga agtgaaaaaa
     4561 ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacgcaaca acacaacatc
     4621 acaaccgtaa gccaaaagtt caccagaata cgcattccga taaaactttt cgttccggct
     4681 caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa tttataatgg
     4741 ctcagattaa aaaaactaat aggttacaca gtgtgatcta attgttaaat tcatttaaca
     4801 tcaaagttta aaagccatgt ctaaacgatt accaccgcta aatgccttac gagtttttga
     4861 tgccgcagca cgccatttaa gtttcactcg cgcagcagaa gagctttttg tgacccaggc
     4921 cgcagtaagt catcaaatca agtctcttga ggattttttg gggctaaaac tgttccgccg
     4981 ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc tatttcctcg atatcaaaga
     5041 gatattttcg caattaaccg aagcgacgcg taaactccag gcccgtagcg ccaagggggc
     5101 gttgacggtc agtttactcc ccagtttcgc cattcattgg ttggttccgc gactttccag
     5161 ctttaattca gcttatccgg gaattgacgt tcgaatccag gcggttgatc gtcaggaaga
     5221 taagctggcg gatgatgttg atgtggcgat attttatggt cggggcaact ggccggggct
     5281 acgggtggaa aaactgtacg ccgaatattt attgccggtg tgttcgccgc tactgctgac
     5341 aggcgaaaaa cccttgaaga ccccggaaga tctggctaaa catacgttat tacatgatgc
     5401 gtcacgccgt gactggcaga catatacccg acagttgggg ttaaatcata tcaacgttca
     5461 gcaagggcca atttttagtc atagcgccat ggtgctgcaa gcggctattc acgggcaggg
     5521 agtggcgctg gcaaataacg tgatggcgca atctgaaatc gaggccggac gtcttgtttg
     5581 cccgtttaat gatgttctgg tcagtaaaaa tgctttttat ctggtttgtc atgacagcca
     5641 ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga
     5701 acaagaaaaa ttccgctttc gttatgaaca ataatttacg tagggtacga ccatgaccag
     5761 ccgttttatg ctgattttcg ccgccattag cggcttcatt tttgtggcac tgggcgcttt
     5821 tggtgcgcat gtgttaagta aaaccatggg ggccgttgag atgggctgga tccaaaccgg
     5881 tctcgaatac caggcgtttc atacgctggc gatcttaggt ctggcggtgg caatgcagcg
     5941 tcgcatcagt atctggtttt actggagtag cgttttcctc gcgttaggca cggtgctgtt
     6001 cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc
     6061 ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt
     6121 aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct gtgccgtccg ggctttgaaa
     6181 aagagtgcgc cgcagaaatt accgataaag ccggccagcg ggaaattttc ggttttgccc
     6241 gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta tcaacctgat gatggcgata
     6301 agttaatccg tgagctgccg ttcagttcat taatttttgc ccgccagtgg tttgtggtgg
     6361 gggaactcct gcagcatttg ccgccagaag atcgtattac ccccattgtc ggcatgttac
     6421 agggtgtagt agagaagggc ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca
     6481 aagagttgct gaaattctgc cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg
     6541 ctggggtgct ggcgaactat gaaacgccga agcgtccggt tgtgcatgta ttctttattg
     6601 caccaggatg ctgctatacc ggttactcat acagcaacaa taattcgccg ttctatatgg
     6661 gcattccgcg cctgaaattt ccggcagagg cgccgagtcg ttccacgctc aaactggaag
     6721 aggcatttca tgtgtttatt cctgcggatg agtgggatga acgcctggcg aacgggatgt
     6781 gggcggtgga tttaggcgct tgccctggcg gctggaccta ccaactggtg aagcgcaaca
     6841 tgtgggttta ttccgtcgac aacggcccga tggcgcaaag tctgatggat accgggcagg
     6901 tgacgtggct gcgggaagac ggtttcaaat tccgtccgac acgcagcaat atctcctgga
     6961 tggtatgcga tatggttgaa aaaccggcta aagttgcggc attgatggcg cagtggctgg
     7021 ttaatggctg gtgccgtgaa accattttca atctcaaact gccgatgaaa aaacgctacg
     7081 aagaagtatc acacaatctg gcgtatattc aggcacagct tgatgaacat ggcataaatg
     7141 ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc
     7201 gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc
     7261 gtctccggcc tgctaccctt tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc
     7321 atcagaaata atgtgcgtta cctcatccag atgcccgatg cgggcttccc ctgaacgatt
     7381 aaatttactt ttatcaatca ataacaacga ttgcgcggca cgttttaata gcatcgattt
     7441 gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg ctgctatcga tcccttcaca
     7501 agaaaaaata aacagatcga tttccagcga tttaagttgg gaaatcagcg agggattgac
     7561 gtaacagcca tatttgcgct caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg
     7621 tttaccgagt tcatggcaaa tcggatggct attggtgaag acctgaatgt tgatgtcagg
     7681 caactggcgt gccagatacc agcaagttga actggcgtct aaggctatca ccatcccttc
     7741 ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta tgcgcataat ggcttttcag
     7801 cctgatgtga aagggatcgc cactgtcttg attttgacgg tggatatatt tagcgcgtcc
     7861 atgattgcgc agaattttac cctgcgtctg taattcattg agatcgcgac gaatggtttc
     7921 tttactgacc tttagctgtt cagagagagc ttccgtggtc aggctggtat ggttcagcaa
     7981 caggtcgact atcgcttgct ggcgtgccgc tttcatattt ttcaccccat cattcgataa
     8041 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     8101 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     8161 ttcataaaac gcaaaacgat tgatacggat gatgtcagga cacggggctt gtagtgaaag
     8221 cgcattacgg taacgtcgtt ctacttcagg atcgagagtg tcaccctcta ccgccgccat
     8281 catcaccagc ggcggggcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     8341 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     8401 gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc tctgccagca ctttcaatag
     8461 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacttcctct ataaattcag
     8521 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
     8581 ggctgttaaa ttcccctacg ccataccaac cgaacagcgc agctcctgcg acggtcgttt
     8641 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
     8701 atgtgttgcg acttcctcca ccgactaaca ataattcata ggctttaaag tgcccgattt
     8761 tttccagcat ctgtagattg cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat
     8821 agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca gccagcgttc tgacacgaca
     8881 ataaatcaca ctgcattttc acaccatccg cgccaggtgc gatcagacga gcttcttcaa
     8941 tcaacatttg ccagggtgtt tcagccgtcc agaacagttt tctcacccat tccagcacgc
     9001 cggatgccag ccattgcata cctgggttat acaaccatgc ctggctatct agttcgctgg
     9061 tggaaccggc gtactgactt aacagcgaag tatcaacttg ggcgctgcga accattaaaa
     9121 tttcccatgt accggaggag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
     9181 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
     9241 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
     9301 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
     9361 ccagcatctg gctggttccg gccatcgtga tatcagtagt gaattcgccg gttaaacggt
     9421 ggttaatcag cgacgaaatg aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
     9481 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg acgccagaaa
     9541 tagcctgcaa ccgctgtgcg gagattaacc gttcaatatt gtccataacc gctgctgttc
     9601 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttattt accagagcgc
     9661 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcattcagtc agttcactat
     9721 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggagtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagagtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggctaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcacc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgtccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga actggcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcagct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ctcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagttc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgctaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tacttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccgg
//
LOCUS       DADKXM010000068        19512 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.068, whole genome shotgun sequence.
ACCESSION   DADKXM010000068 DADKXM010000000
VERSION     DADKXM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.068"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            73..419
                     /locus_tag="JQR05_002380"
                     /pseudo
     CDS             73..419
                     /locus_tag="JQR05_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Hsp90"
     gene            434..1168
                     /locus_tag="JQR05_002381"
     CDS             434..1168
                     /locus_tag="JQR05_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5324542.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1181..2038
                     /locus_tag="JQR05_002382"
     CDS             1181..2038
                     /locus_tag="JQR05_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5324543.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2041..4536)
                     /gene="ptsP"
                     /locus_tag="JQR05_002383"
     CDS             complement(2041..4536)
                     /gene="ptsP"
                     /locus_tag="JQR05_002383"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY5324544.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQSTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(4561..5597)
                     /gene="ypdE"
                     /locus_tag="JQR05_002384"
                     /pseudo
     CDS             complement(4561..5597)
                     /gene="ypdE"
                     /locus_tag="JQR05_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(5597..6680)
                     /gene="ypdF"
                     /locus_tag="JQR05_002385"
                     /pseudo
     CDS             complement(5597..6680)
                     /gene="ypdF"
                     /locus_tag="JQR05_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6695..7942)
                     /locus_tag="JQR05_002386"
     CDS             complement(6695..7942)
                     /locus_tag="JQR05_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY5324545.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMVGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(7964..8290)
                     /locus_tag="JQR05_002387"
     CDS             complement(7964..8290)
                     /locus_tag="JQR05_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY5324546.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(8509..9474)
                     /gene="glk"
                     /locus_tag="JQR05_002388"
     CDS             complement(8509..9474)
                     /gene="glk"
                     /locus_tag="JQR05_002388"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY5324547.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            9678..10934
                     /locus_tag="JQR05_002389"
     CDS             9678..10934
                     /locus_tag="JQR05_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY5324548.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11049..11375
                     /locus_tag="JQR05_002390"
     CDS             11049..11375
                     /locus_tag="JQR05_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY5324549.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(11516..12754)
                     /gene="mntH"
                     /locus_tag="JQR05_002391"
     CDS             complement(11516..12754)
                     /gene="mntH"
                     /locus_tag="JQR05_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY5324550.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13090..14292
                     /gene="nupC"
                     /locus_tag="JQR05_002392"
     CDS             13090..14292
                     /gene="nupC"
                     /locus_tag="JQR05_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY5324551.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14342..16531)
                     /locus_tag="JQR05_002393"
                     /pseudo
     CDS             complement(14342..16531)
                     /locus_tag="JQR05_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(16739..16814)
                     /locus_tag="JQR05_002394"
     tRNA            complement(16739..16814)
                     /locus_tag="JQR05_002394"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16779..16781),aa:Ala,seq:ggc)
     gene            17050..17394
                     /locus_tag="JQR05_002395"
     CDS             17050..17394
                     /locus_tag="JQR05_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY5324552.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            17417..17788
                     /locus_tag="JQR05_002396"
     CDS             17417..17788
                     /locus_tag="JQR05_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY5324553.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLYIDESA"
     gene            complement(17840..19255)
                     /gene="gltX"
                     /locus_tag="JQR05_002397"
     CDS             complement(17840..19255)
                     /gene="gltX"
                     /locus_tag="JQR05_002397"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY5324554.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         4827 a         5225 c         4859 g         4601 t
ORIGIN      
        1 aaagactata ttgccattga gcaggcccgc tttggcgaca agctgacggt tatctatgat
       61 attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggagaac
      121 gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca
      181 gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat cgatccaaag
      241 gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct gctgaatgtc
      301 catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct ggagccgggg
      361 cgttttacat tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag
      421 ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact
      481 gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct
      541 ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat
      601 tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt
      661 tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt
      721 tgactacatt ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga
      781 agcggcctgg caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa
      841 tgacaccatt aatctggtga aagatgagcg aataatcgtc acgccaatta acgatatcta
      901 ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat
      961 gccgatgaac attaccgaat tttgcagcaa actgccgccg tcgcattttt tccgctgcca
     1021 tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac
     1081 ctacattctg cgactgaaag atctggattt tgaagtgccg gtcagccgca gcaaagtgaa
     1141 agaatttcgc cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc
     1201 cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc cgcaactact
     1261 ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga
     1321 ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca
     1381 gcagcgaatg gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccgat
     1441 caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc
     1501 gttttacgat agccgcacca cgtcgttgtt gcatcctgct cgccagatcc agcttcccag
     1561 cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc
     1621 gctggagcag aatatcattc agccactggt gttaagtttg ctgcatcttt gccgcaacgt
     1681 ggtgaatatg ccgccgggta attcgcaacc gcgcggcgat tttctctatc acagcatttg
     1741 taactgggtt caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt
     1801 taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacga tgcgttttat
     1861 cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct
     1921 gtcaattcat gaagtggcac agcgttgcgg ttttccggat agcgactatt tttgtcgcgt
     1981 tttccggcgt cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg
     2041 tcagaaggtt aattctgttt ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa
     2101 caacgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac
     2161 atgattcatc ccttcgttag cacccagcgt cagcatgatc accagttcga cttcgcccat
     2221 ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg aatgacggat
     2281 ccactgagat ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc
     2341 ttcccgctgc cagacatctt cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc
     2401 gaggttgccg cacaggaact ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa
     2461 gatattctcc agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa
     2521 cgcttcaatt tcctgcgcac tgcggcattc acatgcctga cgcgccagtt cccgacacgc
     2581 ctcgctatcc agttgacgaa gctggctttt caccgccgga atacgcgggc tactcatact
     2641 cagctcatcc aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc
     2701 accgcaaatg cctacccatt tgccccgctg atgcgcagtg gtaactattt gctgcaacat
     2761 gcgcaggaac gatggcgtaa tcgggttata tagcggcgat acgcgcgggt tattacgatc
     2821 gaccgcatac agatactggg tcatatcgtt ggagccgata ctgaagaaat cgacctcatc
     2881 gcagaagtgg tcgatgatgt agcacaccga cggcacttcc accatgatcc caagcgtaat
     2941 cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc
     3001 tttcacccat aagatctgat cgaggctgtg aaccatcggg atcatcaact gggcgttgcc
     3061 gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag caaattccgg
     3121 gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa tattcagata
     3181 aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc
     3241 cgccagtagc acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg
     3301 atccatatag agcatttcgg tacggaacag cccgacacct tccgcgccgt tggcaaacgc
     3361 gcctggcgct tccagagcgg tgccgatatt cgccgcaata tcaatacgct tgttatcacg
     3421 ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt
     3481 ctgcgcgacc tgataataac cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca
     3541 ctgggcgtca agcactgcag gttgcccggc ataacgggca atcgcatcca gcggcaagcc
     3601 actcagtacc gggatcgccg aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt
     3661 tttctccaga atcatacccg caagattttt caaatcaagg ctcaaaaact ggcttggggt
     3721 taaatcttca gccaccagaa tggtcggttt ttccagcacc agattgttgc gcggcttcag
     3781 ttccggccag gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc
     3841 gcgcagataa tcgctggcag aagcagatag tttggcgcaa acctgctcca tattgctgat
     3901 gatcgccgcc cccagtccct gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa
     3961 ttcatcatcc tgaatcagcg acaaatgagc gctgaggata gttttgcttt cgccgtcacg
     4021 ctcacgcaat tgctggttca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt
     4081 ggaatcttgc gcactggcgg ggattgcccg ataactgtcg aggctgtcgc tttgtaacag
     4141 ggtcagcgta cccacgccga cgccgcttgc cagcacattg ccgtacagta aatccgggtt
     4201 aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg tcgactgaac
     4261 gctgtcgctg tcgataaagc gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc
     4321 ttcatcgctg ccgctaatgt tcaggctgca actgtcatta aataaggtgc cagttccaat
     4381 caacgccagc gagcttttag catcggcctt tgcgttctgg cgatggttga taaaggtgat
     4441 ttcgctttgc cactggctgc actgttcttt aagttcccac gccggacgag catgtagacc
     4501 gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt aatcaggaga
     4561 tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa taagggcaga
     4621 taaaagttgc tccatctgca aaatgtcgcg gcaatcggca atcgatgccg cgcaatgtcc
     4681 gtggcgggtg gcaggcccca ttaccactgt gggtacgcca gtaccggtta agtgcaccgc
     4741 tccaccgtcc gtgccgccgt tactgaacat atccgcctgc aacggaacgc caatttctgc
     4801 cgccacggtt tcaatccacg cggtaagttt tggcggcgca atcagcgatt tatcgcttaa
     4861 caccagcatc ggcccgttac caatctgacg atggttagcc gcgccataat caaagttttt
     4921 cgcccagcag gcggtatcaa gcacaatggc gacgtccggc gacaccgcgc gggtagcagt
     4981 ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc accagccaca cttccgcagg
     5041 cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc agatagcaac cgaggcgatc
     5101 atcaaaggct tgcccatcac ccgctggtga gggaggacct gaaaagtggt atcgaaggtg
     5161 acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt cataggtgcg cgcgccaatg
     5221 tccacgcgca tggcgctaac gtcattcccc tgccgttcgc cgtcaagcag gcctggaatt
     5281 ttgcgctctt cacgggtggt gatgcgcacc ggctgcagct ggcgggcagc catgcgcacg
     5341 ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga tgctgcgcac cataaatccc
     5401 acttcgtcca tatgggcgca gatcatcacc ttcggacctg tcgattcatt gaggcggatc
     5461 agcaccgatc ccagaccatc aaatcgcact tctttttgca ggcgagccgc ttcttccagc
     5521 aggatctgcc gcacttcctg ttccgaggag gcgatcgcat ctgcctcgct caacgctttt
     5581 aatagcgata aatccattat gcctctcccg tgagcaacac tgttttcggc atggcgtaga
     5641 gcacttctgc gccttgcggg gtaaccagca caacatcttc gatgcgcacg cccccttgcc
     5701 ctagcaaata aatccccggc tccacggtca gcaacatgcc tggctgtagc gtcgtggtgt
     5761 cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc gatagcgtga ccagtgttat
     5821 gaccgaaata gtcgccaaat ccagcctcgg taatgacccg gcgcgcggcg tcgtcaatct
     5881 gctggcagcg cacgccgggg cgaattgcag agattgccgc gagctgtgcc tgcaggacaa
     5941 tctgatagac gtcaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca
     6001 aggtgcgcgt catatcagca gtagccctga tacagcgcac caaaatcgag agtgacaaac
     6061 tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg gcagcgcccc gcgccagccg
     6121 ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct gctggcgcat aaaccactcc
     6181 agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc
     6241 gctccgcgat cggcaatccc acaggcgagg cggattatct ccacctcctc tggcgttttg
     6301 atttgccgca gcacatccgg cgtggcgcta accagtttcg cattgagttc agacttccag
     6361 cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca
     6421 tcggcaatga tttgattgac gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc
     6481 tggtagcctt gcgcacgggc ttccacctca acgaaatagc gcgaatctac cagaatgtgc
     6541 gcactttcac ggctaataac cacatagcct gatccagtgg agatccccag atgcggctgt
     6601 ttgttctgcc gtgaggagag aagcaccgca tccagttgtt gcaccttaag ccagtcgcgc
     6661 agcgaagcga gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc
     6721 ctttgcggaa catcatcaga cgcaggaaca ccaccatcag cgcagtaatg attgccccca
     6781 gcgcgatccc caccatataa acgcccaggt tagtgaccag cggccatgcc cagatagcgg
     6841 attccgggaa ccattgcact gcgcccagcc agacagctgc ggttgagccg acaatcgcgc
     6901 cgaccatata tgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct
     6961 cactaatccc cataaaggcc aggaacatcg cggtcttacc ctgcggataa agttgagcgt
     7021 tgaacaggcg tttgccggtt aaacgacggt caataatggt cgccagaccc agaccaatcg
     7081 gcggaataac gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc
     7141 tgaaggcaac gaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg
     7201 cggcgatccc cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacagtac
     7261 ggataccgcc gttgatccag ccaccaaagg gggtgatgac gtagtacatc gccagcatga
     7321 caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg
     7381 gtaactggat tttctggttc atccatttca caaggtaacc ggcaacgata gaaataatca
     7441 gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat
     7501 cgaagttcag cagttgcgtc ggctgggtag acatcaaccc gccgataaaa ccagccggga
     7561 acgccagttt gccaccgata gagttcgcca caaatgcagc aaacatcgga atggcaaaac
     7621 caaacaacac gccgccgaag gactgcgaca gccaggcaaa tttaagtaag gagaggtcga
     7681 aaccggagaa tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa
     7741 gccagctata agcaatcagc tggctgaaag cgaggatcac accgcccata attaaggtcg
     7801 gcaccatgcg tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt
     7861 tagaggcctg cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg
     7921 cactgcgttt tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg
     7981 caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag
     8041 tgatttcata aacgtcgcgt gattcgaaac gttcgttatc ttccggggta actgccacgg
     8101 aatggatgat gatggtcgct tcggcgatat cctgcgccgt caggcgattc tggatgccgt
     8161 ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccagcg
     8221 cctgagcggc cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt
     8281 tcttactcat cgttaattcc tcgttcagtg atagttgacg agtgaaatta tgaacgtgca
     8341 aaacaaatcg ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg
     8401 gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga aaaattgaat taactgtatg
     8461 atttaaaaga ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg
     8521 acctaaggtc tggcgtaaat gtgcaccgga accgagaaga cccggatagt catggacgat
     8581 gagatacacc ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcggc
     8641 acggaaaccg gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa
     8701 cacgccgcca aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac
     8761 gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt
     8821 aatatctttt ggcttgagat tttctggcag gcggttgtca gctttcacaa ttgcgcgata
     8881 caaattcacc agcccagggc cagaaagcac gcgctccgcc gaaacatgac caatttccgc
     8941 acgcaatatt tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc
     9001 gccttcgccc ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag
     9061 ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg accggttctg cgccaccaaa
     9121 ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt
     9181 aataatttcc agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca
     9241 ggtatggttg gtcattgcca cccagtcacc ggtaattggg caagcgatgg caatacagcc
     9301 gtctttcacc tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg
     9361 gtaatcaagc cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag
     9421 agcaagacgt gcgttggtgc cgcccacatc accgactaat gcatactttg tcattcttca
     9481 actgctccgc taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca
     9541 acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg ggtcacacaa ttactttatc
     9601 gtttcagcac caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc
     9661 cgcataagga atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc
     9721 ccgccgtggc aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct
     9781 cggtattaca gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt
     9841 cacccctctg gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc
     9901 gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccgg
     9961 ttccgccctc tgcgctacct ggacttatcg tagcattaat tctcggtctt gctggcggcg
    10021 tcagcctggg gccggaacat ccgatcatga ccgtcaatat cgcccttgcc gtcgccattg
    10081 gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa
    10141 ccatcggcgc gctgtttggt acacctgttg cggcggcgtt gatattttcg caaaccttaa
    10201 atggcagtag tgaagttccg ctatgggatc gtctttttgc gccgttaatg gcggcagcag
    10261 ctggtgcact tactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt
    10321 acggacagat ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg
    10381 cagcagggat ggtcgccgta tggtgcttac cacggttgca cgcgatgatg aatcaaatga
    10441 aaaatccggt gctcgtgctg ggtattggcg gatttattct cggaattctg ggggttattg
    10501 gtggaccagt ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg
    10561 ctttcagcac cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg
    10621 ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat
    10681 tagggttgat gctgcatgag cacgttcccg ccgttccagc ggcaataacc gtttcctgcg
    10741 ctattctcgg catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg
    10801 cagtcgttgt acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat
    10861 ggctgttatt agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc
    10921 acgataatgt ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg
    10981 gggctttacc tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga
    11041 gaataacaat gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg
    11101 cagcaaatgc aggtgaaatc accctactgc catcgatcaa attacaaatt ggcgatcgcg
    11161 atcattacgg taattactgg gacggtggtc actggcgaga tcgtgattac tggcatcgca
    11221 attatgaatg gcgcaaaaac cgctggtggc gtcatgataa tggctatcat cgtggctggg
    11281 ataagcgtaa agcatatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc
    11341 gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg tcctccaatg gagcacaatg
    11401 cctgatgcga cgctagcgtg tcttatcagg cctacaaacc ggctctttaa cgcgtagggc
    11461 ggataaggcg tttacgccgc atccgccaat agtgccagat gcgacgctca ttcaactaca
    11521 atcccagcgc cgtccccacc aacaaccaga tattcagcgc cacgaccagc actacaatca
    11581 cccagcctgt ctgttttacg cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac
    11641 tggtgaaaat cagcagagga accagcgcca gggcgatacc aaaacttaac agcacctgac
    11701 tcataaccag aatccgtgtc ggatctaatc ccatcagaat gacaataaat gacggcaaca
    11761 tggtgactgt acgacgcacc cacagcggga tatggaagcg aatgaatccc tgcatcacca
    11821 cctgccccgc cagtgtcccc accaccgttg aggacagtcc ggcagcaacc agacttaacc
    11881 caaagaccgt tgccgcagca tggcttaaca gcggttgcag cgtcagataa gcctcatcaa
    11941 gatcggcaac accagtatga ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg
    12001 ccagattgac aaaaccggca atcgtcatgg cgatagccac atcccatttg gtggcggaat
    12061 aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag cgaagagtgc aaataaatca
    12121 catgcggcat aatcgtcgcc cctaacacgc ctgctgccag gaagaccgcc tccgaagtag
    12181 gtaaactcgg gatcaccatt cctttaccca gctgcgccag gttaggctgg gagaaaatca
    12241 actcgacaat gtaagccgcg gcaacaaaca acagtaaccc gccaatcact ttctccagcg
    12301 gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg
    12361 cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc gatcgccgca ccaataaatt
    12421 ccgccaggtc ggttgccatc gcaataattt ctgcctgaac ccaatagaac cacactacgg
    12481 gacgcggata gtgatcacga atctgctccg ccagattttt accggtggca atccctagtt
    12541 tggcagagag gatctgaatc agcatcgcca tcaggttggc ccaaacgaca acccacagta
    12601 gctgatagcc gaagctagca cccgcctgaa tattggtcgc aaagttaccg ggatcgatat
    12661 aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc
    12721 gtccgctgct actctcaacg cgatagttcg tcatcttgtg cctctaaaac atagcctttg
    12781 ctatgtttca tgctatgcta aacgagaatg attatcaaat tcatttaaat ggattgtggt
    12841 gatttctctg atagaccagg attatgatta cgaaaagatt gatgacttga atgttatggt
    12901 tatgtttaat gttagcacat ttacataact ttcagcttcc atacacaaca tagcagaaat
    12961 gtatgacaga tcactatttt tgaagcctgt cacaggacgt cattatagtg tgtgtcagat
    13021 ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa atttaacctg cctcatattt
    13081 ggagcaaata tggaccgtgt ccttcatttt gtactggcac ttgccgttgt tgctattctc
    13141 gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt
    13201 gttatcgaag tgttactggc gtggttcttc ctgaactccg acgttggttt aggcttcgtg
    13261 aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg ccaacgaagg gactaacttc
    13321 gtctttggta gcatgaatga tcaaggcctg gcattcttct tcctgaaagt gctgtgccca
    13381 atcgtcttta tctctgcact gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatt
    13441 atccgcgcaa ttggtttcct gctctccaaa gtcaacggta tgggcaaact ggaatccttt
    13501 aacgccgtca gctccctgat tctgggtcag tctgaaaact ttattgccta taaagatatc
    13561 ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg ccacggcaat gtccaccgtg
    13621 tcgatgtcca tcgttggtgc atacatgacc atgctggaac cgaaatacgt cgttgctgcg
    13681 ctggtactga acatgttcag cacctttatc gtgctgtcgc tgatcaatcc ttaccgtgtt
    13741 gatgccagtg aagaaaacat ccagatgtcc aacctgcacg aaggtcagag cttcttcgaa
    13801 atgctgggtg aatacattct ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg
    13861 atcggcttta tcgccctgat cgccgcgctg aacgcactgt ttgccaccgt tactggctgg
    13921 tttggctaca gcatctcctt ccagggcatc ttgggctaca tcttctatcc gattgcatgg
    13981 gtgatgggtg ttccttccag tgaagcactg caagtgggca gtatcatggc gaccaaactg
    14041 gtttccaacg agttcgttgc gatgatggat ctgcagaaaa ttgcttccac gctctctccg
    14101 cgtgctgaag gcatcatctc tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg
    14161 attatcgcag gtgcagttaa aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc
    14221 ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca
    14281 ctggtgctgt aaaaccatac ataaaaaagc cggggatcat tccccggctt ttttacgcct
    14341 gttaatcagc taatggctgc gggcgaccaa tcaaataccc ttgcagatat tgcaccccga
    14401 gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg
    14461 acttcgcttt cgccagatcg gtaattgatc tcacaatcat cgcatccagc gtattcgtga
    14521 caatatcttt cacaaagacg ccatcaattt tgatgatatc agcctgcaaa cgctttaacc
    14581 gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc aatccggaaa ccaaacttat
    14641 gcagctgctc gatgttgtac atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga
    14701 tctcaagaat gaccgcctgc ggggagatgt gataacgctt aaacagacga ataatccgcc
    14761 cggcaatatt cttttgcagc agcgtgagcg gcattaaatt gactgaaaag cgcggtcctt
    14821 ttttgtcgca agggtgtgtt gccagccact tcaacaagga ttccagcact tgcaaatcaa
    14881 aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa cttatccggg gtcataatgc
    14941 cgccgtcata tttcagtcgc gcgaggatct catcataacc ttcaccctct ttgttgcgaa
    15001 ttggctgggc gtagagcagc aaatcaccct gatctaacgc cgtgcgaatg gtattcagca
    15061 atagcacctg tttagtggtc tgcccggaaa ccatctcctc tctgctatcc agcgccagca
    15121 catgatgatg tgcgcaggat tgctccgcca gccagcttaa ctgccccaac aagggttgct
    15181 gggtttcctg atttccatca aaacgccccc aggcagcacc atagcccata tccagcccgg
    15241 tattgttcca gtgaatttgc cgactattca ggatgttaac catatgctgg agtcgccctt
    15301 ccgtttccgg cccgctcagc actaacagca gttcactacc cggcaattga tacaactttt
    15361 cgttttcctg catcaacggc agcagcgtac ggcaaattga gcggatacag tgaacgcgca
    15421 tcattaagcc gtaatgacga ctcataaact caagattatc aatgcgcagg cagcaaaaac
    15481 tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg aaagttgggt aggagcgtta
    15541 acggatctgt cagcgcctgc aaatgccagc ggcgattaag ccattcactg cgatgataaa
    15601 tgcgcaccat atagagcagg caaacgctaa aggaaatcag caccgcgaga ataaatgcca
    15661 gcgaatattc ggtttccacc ccttacaaaa agttctggtt gtaattcaga aggcaaagcg
    15721 ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag attaccgacc ccgagggtaa
    15781 agatgatgaa gaaaacgggt accaggtaac cggcaataaa gtcgttttca taaggtgtgc
    15841 acaacagaag tagcagcacg cttaacgctg ccagccagct taaggtaaat gcgcgtttct
    15901 ctttgcccaa cgacggagcg atatccctgc gccacaatat ctgcgcaaag tggggactta
    15961 caatcatgcg agtgagatag tagaaaagca tgttgtaaat cagcactgcg gtgaaaaggc
    16021 ttagtaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggtg gatatcttta
    16081 gcgggaaatc aaagaagctc cccacaagat acatgctgca tttgatgccg atcggcgtca
    16141 ccaaaccaag ccagaacaga cgttgccaga catatcggct ggtcaatcca taacgccaac
    16201 gcgtccccag ctgccagcgt aatattgcgc aggcacacaa cacgacaaac gtctggctgc
    16261 cgagcatgac cgcagtctgt aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca
    16321 tcccgcataa aacgggaacg acaccgcgcc agccaaagat aaacaagatt gacagcatta
    16381 cgcacagtgg catccacgcc agaaagatat aactggaatg gataatggca agtggcgaaa
    16441 taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga
    16501 tattttttat caggttatgc tccacaaaca tgagtacact actctcactg atcggggttt
    16561 tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc agcctgttgc
    16621 cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta gactgtatta
    16681 caaaagcggc aaaaagcaga gacaaaaaac ccccgctttg cagcgagggt tggaaatttg
    16741 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    16801 tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc
    16861 gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa
    16921 atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa
    16981 agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc
    17041 aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt
    17101 aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt ccagggtggc
    17161 aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt
    17221 ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg
    17281 gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg
    17341 cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa
    17401 aagattacgc aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca
    17461 aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa
    17521 aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac
    17581 gccagccttc cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc
    17641 tcctggagca cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga
    17701 cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc
    17761 ccgtctctat attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt
    17821 ttatcttttt acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt
    17881 tgttgatacg ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg
    17941 gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta cccataccca
    18001 cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag
    18061 taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat
    18121 gttttttcgc cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct
    18181 gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct
    18241 gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccattgtaag tgagtagcaa
    18301 catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt
    18361 tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac
    18421 gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg
    18481 cttctggcaa ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg
    18541 acagtttttt accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg
    18601 ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac
    18661 ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg
    18721 gggaaccatc ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg
    18781 gaccacggat ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa
    18841 cacacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg
    18901 gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat
    18961 agcatttata agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa
    19021 aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat
    19081 ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca
    19141 gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc
    19201 cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat
    19261 ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat
    19321 attctatcac tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg
    19381 ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga
    19441 cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc tataatacga
    19501 ctccacacag cg
//
LOCUS       DADKXM010000069        19368 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.069, whole genome shotgun sequence.
ACCESSION   DADKXM010000069 DADKXM010000000
VERSION     DADKXM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.069"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JQR05_002398"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JQR05_002398"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY5324555.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JQR05_002399"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JQR05_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY5324556.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JQR05_002400"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JQR05_002400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY5324557.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JQR05_002401"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JQR05_002401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY5324558.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JQR05_002402"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JQR05_002402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY5324559.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JQR05_002403"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JQR05_002403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY5324560.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JQR05_002404"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JQR05_002404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY5324561.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JQR05_002405"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JQR05_002405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY5324562.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JQR05_002406"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JQR05_002406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY5324563.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JQR05_002407"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JQR05_002407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY5324564.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JQR05_002408"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JQR05_002408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY5324565.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JQR05_002409"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JQR05_002409"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY5324566.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JQR05_002410"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JQR05_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY5324567.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JQR05_002411"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JQR05_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY5324568.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JQR05_002412"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JQR05_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY5324569.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="JQR05_002413"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="JQR05_002413"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY5324570.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JQR05_002414"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JQR05_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY5324571.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSQEAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JQR05_002415"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JQR05_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY5324572.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELQEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPADWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4623 a         5124 c         4853 g         4768 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttatcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcatcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccattatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcgag tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg gctttcgtgc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgataataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctggggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gttcaacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttca
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaagaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgtt gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaagcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaatt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgatattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacaaccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 agtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 tgcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacgctaa agtcgatggt
    13201 tcgagcatca atacgcggcg gagtaccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagagagcg gaatggatgg cggatcgcca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt tgccgtcgag gaacgtccca acagtgagca ccacagcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagcgca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcagccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg acttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa cagcggttgt tctttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actgccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aaccagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gctgatcagg gcttgatggt acgcatgatc
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca taacttttat aaagatgctg
    14881 tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 ttcttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac cgttcctgcc ggatcagatt
    15841 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    15901 gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg
    15961 agtgatggtc atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggcc atgcgggcct gaacggcgat attctggtgt ggaacccggt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata acagcgccgg ttttcagcga
    16741 gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg agcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcctgag aacgccccag ctgctctgcc aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctgacc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 aacgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg gcagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    18241 acgacggctg aaaatgccgc ccagacgaat taacgttaag gctgttttat cgctttgttg
    18301 ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg
    18361 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatca accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cttgcacttt atctaaccat agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc ctgcagctcg ttggaggccg ccaccagtag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacgatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DADKXM010000070        19074 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.070, whole genome shotgun sequence.
ACCESSION   DADKXM010000070 DADKXM010000000
VERSION     DADKXM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.070"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..343)
                     /locus_tag="JQR05_002416"
     CDS             complement(<1..343)
                     /locus_tag="JQR05_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY5324573.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNHVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYS"
     gene            complement(343..1584)
                     /gene="clsB"
                     /locus_tag="JQR05_002417"
     CDS             complement(343..1584)
                     /gene="clsB"
                     /locus_tag="JQR05_002417"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY5324574.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(1581..2342)
                     /locus_tag="JQR05_002418"
     CDS             complement(1581..2342)
                     /locus_tag="JQR05_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY5324575.1"
                     /translation="MPNQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            2475..2885
                     /locus_tag="JQR05_002419"
     CDS             2475..2885
                     /locus_tag="JQR05_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324576.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(2847..3953)
                     /locus_tag="JQR05_002420"
     CDS             complement(2847..3953)
                     /locus_tag="JQR05_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5324577.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(3964..5097)
                     /locus_tag="JQR05_002421"
     CDS             complement(3964..5097)
                     /locus_tag="JQR05_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5324578.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGMPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(5090..6826)
                     /locus_tag="JQR05_002422"
     CDS             complement(5090..6826)
                     /locus_tag="JQR05_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5324579.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(6819..7817)
                     /gene="hlyD"
                     /locus_tag="JQR05_002423"
     CDS             complement(6819..7817)
                     /gene="hlyD"
                     /locus_tag="JQR05_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY5324580.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPMTVQFGDEAGHE"
     gene            complement(7817..8488)
                     /gene="cecR"
                     /locus_tag="JQR05_002424"
     CDS             complement(7817..8488)
                     /gene="cecR"
                     /locus_tag="JQR05_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY5324581.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            8717..10081
                     /gene="rhlE"
                     /locus_tag="JQR05_002425"
     CDS             8717..10081
                     /gene="rhlE"
                     /locus_tag="JQR05_002425"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY5324582.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(10313..10795)
                     /gene="ybiA"
                     /locus_tag="JQR05_002426"
                     /pseudo
     CDS             complement(10313..10795)
                     /gene="ybiA"
                     /locus_tag="JQR05_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            10915..13065
                     /gene="dinG"
                     /locus_tag="JQR05_002427"
     CDS             10915..13065
                     /gene="dinG"
                     /locus_tag="JQR05_002427"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY5324583.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIAKKKEKTKSPRRRRR"
     gene            13093..14055
                     /gene="ybiB"
                     /locus_tag="JQR05_002428"
     CDS             13093..14055
                     /gene="ybiB"
                     /locus_tag="JQR05_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY5324584.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVVKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            14196..15281
                     /locus_tag="JQR05_002429"
     CDS             14196..15281
                     /locus_tag="JQR05_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY5324585.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(15510..15770)
                     /locus_tag="JQR05_002430"
     CDS             complement(15510..15770)
                     /locus_tag="JQR05_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5324586.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16035..16301)
                     /gene="ybiI"
                     /locus_tag="JQR05_002431"
     CDS             complement(16035..16301)
                     /gene="ybiI"
                     /locus_tag="JQR05_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY5324587.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(16375..17088)
                     /gene="ybiX"
                     /locus_tag="JQR05_002432"
     CDS             complement(16375..17088)
                     /gene="ybiX"
                     /locus_tag="JQR05_002432"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY5324588.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17094..19074)
                     /locus_tag="JQR05_002433"
                     /pseudo
     CDS             complement(17094..19074)
                     /locus_tag="JQR05_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4375 a         5040 c         5201 g         4458 t
ORIGIN      
        1 gggagtacaa acgatagcgc ataccaatgc cgccaaccca ggtactgagc gtcaggttga
       61 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
      121 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
      181 ccgcactaag cagcgcaacg tgattgtagt cgcggatgac cttccagacc tcttcccagt
      241 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
      301 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcagggtt ttacccccgt
      361 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
      421 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
      481 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
      541 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
      601 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
      661 gagattactg gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag
      721 cgggcggcgg cggtactcaa aaacctgaac gccgccttta accagatagt tatacagcaa
      781 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
      841 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
      901 ggcgttggcg attatcactt cccgccgcgc ctgagtgagc attttcagat aatggcgttc
      961 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
     1021 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
     1081 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
     1141 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
     1201 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
     1261 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
     1321 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
     1381 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
     1441 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
     1501 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
     1561 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
     1621 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
     1681 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
     1741 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
     1801 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
     1861 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
     1921 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
     1981 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
     2041 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
     2101 aaatcgctcc acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
     2161 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
     2221 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
     2281 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgattgggc
     2341 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
     2401 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
     2461 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
     2521 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
     2581 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
     2641 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
     2701 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct atggctgttg tcccgcgtac
     2761 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
     2821 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
     2881 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
     2941 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
     3001 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
     3061 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
     3121 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
     3181 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
     3241 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
     3301 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
     3361 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
     3421 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
     3481 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
     3541 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
     3601 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
     3661 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
     3721 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
     3781 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
     3841 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
     3901 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
     3961 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
     4021 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
     4081 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
     4141 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
     4201 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
     4261 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
     4321 accgcgatac ggcatgccga gaataaacac tgacaccagc atacacagca acatcgccag
     4381 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
     4441 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
     4501 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
     4561 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
     4621 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
     4681 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
     4741 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
     4801 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
     4861 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
     4921 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
     4981 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
     5041 cgtctctttg acgcacagcg cccgcacgcg acgccaggaa aggatcgggt tactcattgc
     5101 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
     5161 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
     5221 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
     5281 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
     5341 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
     5401 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
     5461 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
     5521 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
     5581 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
     5641 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
     5701 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
     5761 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
     5821 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
     5881 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
     5941 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
     6001 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccgtcgc
     6061 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
     6121 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
     6181 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
     6241 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
     6301 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
     6361 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
     6421 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
     6481 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
     6541 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
     6601 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
     6661 atcccgccaa catccgcatc agcgtggttt tacctgcacc gtccggcccc accaaccccg
     6721 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
     6781 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
     6841 ccgaattgta ccgtcattgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
     6901 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt ttttggggta
     6961 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
     7021 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
     7081 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
     7141 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
     7201 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
     7261 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
     7321 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
     7381 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
     7441 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
     7501 cgatacccgg caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc
     7561 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
     7621 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
     7681 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
     7741 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
     7801 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
     7861 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
     7921 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
     7981 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
     8041 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
     8101 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
     8161 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
     8221 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
     8281 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
     8341 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
     8401 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
     8461 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
     8521 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
     8581 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
     8641 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
     8701 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
     8761 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgcggt
     8821 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
     8881 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
     8941 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
     9001 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
     9061 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
     9121 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
     9181 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
     9241 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
     9301 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
     9361 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
     9421 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
     9481 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgcggcgat
     9541 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
     9601 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
     9661 gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
     9721 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
     9781 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
     9841 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
     9901 cggcggcggt cgtgggcaag gtggtggtcg cggtcaacag caaccacgcc gtggggaagg
     9961 tggcgcaaaa tctgcaagcg cgaaacctgc agaaaaaccg tctcgccgcc tcggcgatgc
    10021 caaaccggca ggcgaacaac aacgtcgccg ccgtccgcgt aaacctgccg ctgcgcagta
    10081 atcttttatg ccgggctatg cccggcatca ggctgatgaa caaacgcaaa actgcctgat
    10141 gcgctacgct tatcaggcct acgtgaactc tgcaatatat tgaatttgca tgcttttgta
    10201 ggccggataa ggcgtttacg ccgcatccgg catttcacaa caagcacttg tcagcaattt
    10261 gagaacacgg gaaaaaattt atctgtttta ccgcccataa gccaccgaga cgttacttct
    10321 ctatagccaa ttgttcgcgc aactccatta aaaggtagcc cagtcgattc ttgcccttac
    10381 cattaccacc gtctccccag taagcatcgt tttccgtatg ctcaaccagt tttgcgggcg
    10441 cggttgccag caagagcaca cgcagttctg catgctgttc gaatttagcg cgaagtgctt
    10501 tacgcatcac ctgttcttta accgactccc agtttttacg aagtggcttg gaacgattac
    10561 gccccatgcg agcggcgacc atgggggaag aaacccgacg gatctcttcg cggtattttt
    10621 catcaaggaa tttttgtgcc tgaaaatagt gttcggaggt gggctaggta ttcccgtcaa
    10681 ccttgatggg ccaggcggca aagttagaaa aatcaccgta gtcatcgctg gtgctgtaga
    10741 aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg tgctcgaacg ggcattaaac
    10801 cgcattatgt tggtggttat tgcgagccgc tttccagaaa cagaaaaacc attacccctg
    10861 aaaaccgaaa aatgccacaa tattggctgt ttatacagta tttcaggttt tctcatggca
    10921 ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata aggcgcttca ggaacagatc
    10981 cccgacttta ttccccgtgc gccgcagcgg cagatgattg cggacgtcgc caaaacgctg
    11041 gccggagaag aagggcggca tctggcgatt gaagccccca ccggcgttgg gaaaacgctc
    11101 tcctatttga tccccggcat cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt
    11161 accgccaacg tggcgttgca ggatcagatt tacagcaaag atttaccgct gctgaaaaag
    11221 atcattcccg atcttaaatt cactgccgct ttcgggcgtg ggcgctacgt ttgtccgcgt
    11281 aatctgacgg cgctcgccag cactgaaccc acgcaacagg atctgctggc gtttcttgac
    11341 gacgaactga cgccaaataa tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat
    11401 ctcgacactt ataaatggga tggtctgcgc gatcatacgg atatcgctat agatgatgat
    11461 ctctggcgtc gtttgagtac cgacaaagcc agctgcctca accgtaactg ttactactat
    11521 cgtgaatgcc cgttttttgt tactcgtcgg gagattcagg aagcggaagt ggtggtggca
    11581 aaccatgcgc tggtgatggc ggcgatggaa agcgaagccg tactgcctga cccgaaaaat
    11641 ctgctgctgg tgctggacga aggtcatcac ctgccagatg tggcccggga tgcgctggag
    11701 atgagcgctg aaatcaccgc gccctggtat cgactacagc tggacttgtt cacgaaactg
    11761 gtcgctacct gcatggagca gtttcgcccg aagaccatcc caccgctggc gatccctgaa
    11821 cgtttgaatg cgcattgtga agagttgtat gagcttatcg cctcgttaaa caacattctc
    11881 aatctctaca tgcctgccgg gcaggaggca gagcaccgtt ttgcgatggg cgaactgcct
    11941 gatgaattgc tggagatctg ccagcggctg gcaaaactca ccgagatgct gcgtggtctg
    12001 gcggagttat ttcttaacga tttaagtgag aaaaccggca gccacgatat tgtgcgtctg
    12061 catcggttga ttttacagat gaaccgcgcg ctggggatgt ttgaggtgca aagcaaactc
    12121 tggcggctgg cgtctctggc gcaatcttcc ggtgcgccgg tgaccaaatg ggcgacgcgg
    12181 gaagagcgcg aagggcagct acacctctgg tttcactgcg tgggaatacg tgtcagcgat
    12241 cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg
    12301 cgttcgctga atagtttttc gcgtttgcag gagatgagtg gtctgaaaga gaaagcgggc
    12361 gaccgttttg tggcgctgga ttcccccttt aaccactgcg aacagggcaa aattgttatt
    12421 ccccggatgc gctttgagcc ttccatcgac aacgaagagc aacacattgc cgaaatggcg
    12481 gcctttttcc gtgaacaggt ggagagcaaa aaacatctcg gtatgttggt actgtttgcc
    12541 agcggacggg cgatgcagcg ctttctcgac tatgtgacgg atttacgcct gatgttgctg
    12601 gtgcagggcg atcagccgcg ttatcgctta gttgaactgc accgcaaacg cgttgccaac
    12661 ggtgagcgta gcgtgctggt gggcttacag tcatttgccg aagggcttga tttgaaaggt
    12721 gatctgctca gccaggtgca tatccacaaa atcgcttttc cgcccatcga cagcccggtg
    12781 gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct atccgtttga ggtgcaaagc
    12841 ctgccgagcg cctcgtttaa cctgattcag caggttgggc gactgattcg aagccacggt
    12901 tgctggggcg aagtggttat ctacgacaaa cgcttgctga caaaaaatta tggcaagcga
    12961 ctactggatg cattaccggt atttccgata gagcaaccgg aagtccctga aggtatagct
    13021 aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc gttaatgatg tgagtcaggt
    13081 aaggagtcgt aaatggacta tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac
    13141 gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg ctcatatgct caatggtgaa
    13201 gtccctgacc ttgagttggg cggcgtattg attgcgctgc gtatcaaagg ggaaggggaa
    13261 gcagagatgc tcggctttta cgaagccatg caaaatcaca ccatcaagct gacgccgcct
    13321 gcaggcaagc cgatgccgat tgtcatcccc agttacaacg gcgcgcgtaa acaggctaac
    13381 ctgacgccat tattggcgat tctgctgcat aaactcggtt ttcctgtggt ggttcacgga
    13441 gttagcgaag atccaactcg cgtgctgaca gaaaccattt tcgaactgat gggtattaca
    13501 ccaacgttgc acggcgggca ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg
    13561 cctgtcggcg cgttttgccc gccgctggaa aaacaactgg cgatgcgctg gcggatgggc
    13621 gtgcgtaaca gtgcacatac cctggcgaaa ctggcgacgc catttgccga aggtgaggca
    13681 ctgcgtcttt ccagcgtttc gcatccggaa tacattggac gcgtcgtgaa gttctttagc
    13741 gatatcggcg ggtgggcgtt actaatgcat ggtactgaag gcgaagtgta tgctaatccg
    13801 cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga tgcgggtgct gtatgaaaaa
    13861 caggaccctg ctggtagcga gttactgcca caagcaaaag atccggaaac cacggcgcag
    13921 tggactgagc gttgcttggc tggcagcgaa gcgattcccg aatcgctgaa aatccagatg
    13981 gcttgctgcc tggtggctac gggtgaagcg gcaactatca gcgatggcct ggcgcgcgtt
    14041 aatcaggcat tttgattttt atctcagccg gatgagccac gtcatccggc ttttacccca
    14101 ccgcaatatg aaattcctgc atctttattg accttcccgc gcccggcgtg cagcataaaa
    14161 aaacaacaaa cacataacat aaacaggagt taaccatgga aagtggtcat cgctttgatg
    14221 ctcagacgct gcacagtttt attcaggctg tatttcgtca gatgggtagc gaggaacaag
    14281 aagcgaaatt agttgccgat catttaatcg cggcaaacct ggcagggcat gattctcatg
    14341 gtattggcat gatcccaagc tatgtgcgtt cctggagtca ggggcatctg caaattaacc
    14401 atcatgccaa aatcgttaaa gaggcggggg cggcagtcac gctcgatggc gatcgcgcat
    14461 tcggtcaggt cgcggcacac gaagcgatgg cgctggggat tgagaaagcg catcagcacg
    14521 gtattgctgc cgtggcgcta cataactcgc atcatatcgg ccgtatcggt tactgggcgg
    14581 agcagtgttc agcggcgggg tttgtctcta tccactttgt tagcgtggtc ggtattccaa
    14641 tggtcgcgcc gttccacggt cgcgacagcc gctttggcac caatcccttc tgtgtggttt
    14701 tccctcgtaa agataatttc ccgctgttgc ttgattacgc caccagcgcc attgcatttg
    14761 gcaaaacccg cgtcgcctgg cataaaggcg tccccgtgcc gccaggttgc ctgattgacg
    14821 ttaacggcgt gccgacgacc aatccggcgg taatgcagga gtcgccgttg gggtcgctgt
    14881 tgacctttgc cgaacataaa ggctacgccc ttgcagcgat gtgtgaaatt cttggcgggg
    14941 cgctttctgg cggtaaaacg acgcatcagg aaacgttgca aaccagcccc gatgccattc
    15001 ttaactgcat gaccactatc atcatcaacc cggaactgtt cggcgcgccg gattgtaacg
    15061 cgcagaccga agcctttgcc gagtgggtga aagcctcgcc gcatgatgac gataagccga
    15121 ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga acggcaggag caggggattc
    15181 cattggatgc gggaagctgg caggccattt gtgatgcggc gcggcagatt ggtatgtcgg
    15241 aagagacgtt gcaggctttc tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg
    15301 cggacgggca aacaagggta acataggatc aatgagggtt agagcatatg cgtctgtcgg
    15361 caaacagaca gggaaatact tgtgctggac gtagcgtaaa cgcctgatcc gtccaacggt
    15421 tcggattttg taggcctgat aagacgcggt agcgtcgcat caggcatctg ttgccggatg
    15481 cggcataaac gccttatccg tcctacgaat tacttataaa ttaccgcagt accgcttaat
    15541 ttattgttgt tggtggcgga agtgatgctg tatccactgg caccggctgc cgccgctttc
    15601 tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc catcggcaga aaccacgccg
    15661 attttattca tgttctgtgc ctgggatgcc gttaccggtt ccgccgcgaa cacgccaaat
    15721 gacagagttg aaagagccat agcagcaaca acagtattga tagttttcat aattaatctc
    15781 tcgcaggtga tttttgtata agtgacgttg tttcgtcgat gtgatgagta tcacgttttt
    15841 ttacgagaga taaaatcgaa gagaattgac cgccttgttc aaataaattg attgatatct
    15901 aacatactga taaaccattt atttgttgta cttatggact tgttttatgc cgcgtggcag
    15961 tcacagcagc gttaaaggca cggaatgcca cgcaaaatac aaattatact cgctatgcat
    16021 ctcgccagtt gtagttaacg taactggctg tctttcgaac ctctgcgatt atatcctgta
    16081 tacgctggtt tttgtaaatc tttctcctgc tgacaatgaa tacataagcg cacgccagga
    16141 atggcttccc gacgggcttg cgggatgggg gctccgcact cttcacattc atacaggctt
    16201 tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat cttcaattgt actgttgatc
    16261 tgttcgttga cggcgtcatc gttagcccaa gcggatgcca tatcgtcctc cccatatcaa
    16321 tgcttgtaca gttaagtgta gctaatatag ggacgaacct gggcagttca agcgtcagat
    16381 ctccgaccat tcccgcagca gattatgata aagattgagc agcgacagga tctcttcatt
    16441 ttcaccatag cggcttttca gcgactgaat atttttgtcc agttcaaaca gcatggcgcg
    16501 ctttttatca tcgcggatca tcgactggat ccacataaat gatgccactc gtacgccgcg
    16561 ggttacgggt gtcacgcaat gcaggctgct ggagggatac aacaccagat cacctgccgg
    16621 gagttttacc cgatgttgtc caaaggtgtc attaacgacc agttcgccgc cgtcgtagct
    16681 ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagtg cgcatccagc cgttttgtgg
    16741 atggctgcgt actgcgccat ccacatggaa accataggtt tcattgttct gatagcgatt
    16801 aaacagcggc gtggaaaggg tacgcggcaa ggcagcggca aagaacaatg catgttggtt
    16861 aaccgcgttc agcacctcat tttgcagtgc ggcgtataac gcgctgcggg tgtctacttg
    16921 ctgattgttc ttaacttgtg caccttgtgc tccggtggtg acgcgtccat ccacccattc
    16981 ggcttgttcc agttgttcgc gaaaatgggc gacgtcctgt ggcgataaca cgccgggaat
    17041 gtggtacatc atacaagttc tccaaaaagt ggggcctgcg ccccacatct gaatcagaaa
    17101 tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg gtagccgctc
    17161 ttgttgattg aggcgacgta atcggtatca aacaggttgt aaacgtttag ctggaagtcg
    17221 agattgcgat taacccgata ccccagtttg gcatcggcga cccagtaacc ttctgtaaat
    17281 gctggtgttc ccaccgcgcc gtctgaacct ttatgtatac tgccgatata gcgtgcgccc
    17341 gcgccaacag agatatcatc ggttgcctga tattggctcc ataaggtgaa ggcgtgctcc
    17401 ggggtatacg gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt tttgatggtt
    17461 gctttttgct gggtatagcc gccaatcacc tgccacgcgg gagtgatatt ccctgccacg
    17521 gatatctcat agccttcgac gcgtttctta ccgtattgcg agtaagtccc gtcatcattt
    17581 tgctcaactt cattttcgat atcagtacgg aacagcgcgg cggtgagcaa caggcgttta
    17641 tccagaacct gccatttggt gccaatctcg ctggtgttgg ctttttgcgg tttaaaatcg
    17701 gtgcggttgg cactgttacc actgccagac tgcgcaaggg cgaagttgtt gccgcccgga
    17761 ggctgctggg aaacggcata gttaatatag acattgccgt tttccgtcag gtgatacagt
    17821 gccccggctt tccagttcac cagattgccc gacttggcgg tgtcgacggt ggtgactgga
    17881 gaaccttttg ccacaccagc tgggcaggtg atggcaccgc gtccgctgcc gccgcaggcg
    17941 gtggcactgt catattcagt atgataatta tccagacgga tcccgccgtt cagctcaaaa
    18001 tcacgggtga tttgcagcgt atcgaaggcg taaattgcga aggtatccgt ctgaccattg
    18061 gcgtttgcgc cgttgcgcgt caggccgccg ggatgaattc tgctgtcagg atgataaata
    18121 tttaccgccg gtaacgtcac cggattaacg ccatagttag tctgcgtttc acgggtaaat
    18181 tccacgccgg tactgacatc atgaccgata gaaccggtat agaacgtcga ggtcaggttg
    18241 gtctggttgg tgagaatttt attactcaca tctttggtat tcgccgtgcg tgactaggtc
    18301 cagctattca catcgctggt gggctgagta atattcgacg ccccgcccat aatcgccgtc
    18361 atcaggtaat cctgctttac gcgcgaccaa cgggtagtat tgcgaatggt ggtgttatcg
    18421 ttgatgtcgt gctcaaaacg catggtggcg gtgtcggtgg tcgaatcgtc gtaatcggaa
    18481 tccgtgccgt aaaagttatg agtatcaact tttccggaat gattcagggc cgccgctccc
    18541 gcagatgggg cagaatagcc cggcaaaccg atggtcggaa tgccgccgtc tggcgtgttg
    18601 tgctgagtga catgcagata attaagatac aaacgattcg ctgtaccaag gccaaaagcg
    18661 acagaagggg cgacgccgta acgctcattt ttgactttgt cgcgtccggc atcgtgggtt
    18721 ttttcgccca tcacattcag gcgcaccgcg gtagtatcac caatgacctg attgacgtcc
    18781 agcgtgccgc ggcggaacta ggcgctgcca atactggcgg aggcgtcaat gccggaatca
    18841 ttgcgcggct gcttgctgat catattgatc gagcctgtcg gtgcgctgcg cccgtagtcg
    18901 gtgccggacg gccctttaat cacttcgacc tgctcggtat tgaaggtgtc gcgcgagacg
    18961 ctgccgatat cgcgaatacc atcaatataa atactgttag aggtatcggc accacgcata
    19021 taaatggcgt cgccagtggt ggagttaccg ttctcacccg caaaaaacgc accc
//
LOCUS       DADKXM010000071        18808 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.071, whole genome shotgun sequence.
ACCESSION   DADKXM010000071 DADKXM010000000
VERSION     DADKXM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.071"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..699)
                     /locus_tag="JQR05_002434"
     CDS             complement(<1..699)
                     /locus_tag="JQR05_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5324589.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFI"
     gene            806..1102
                     /locus_tag="JQR05_002435"
     CDS             806..1102
                     /locus_tag="JQR05_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5324590.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1236..1661)
                     /gene="arsC"
                     /locus_tag="JQR05_002436"
     CDS             complement(1236..1661)
                     /gene="arsC"
                     /locus_tag="JQR05_002436"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY5324591.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1674..2963)
                     /gene="arsB"
                     /locus_tag="JQR05_002437"
     CDS             complement(1674..2963)
                     /gene="arsB"
                     /locus_tag="JQR05_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY5324592.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3017..3370)
                     /gene="arsR"
                     /locus_tag="JQR05_002438"
     CDS             complement(3017..3370)
                     /gene="arsR"
                     /locus_tag="JQR05_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY5324593.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4113..4196
                     /locus_tag="JQR05_002439"
     CDS             4113..4196
                     /locus_tag="JQR05_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY5324594.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4250..5602)
                     /gene="gorA"
                     /locus_tag="JQR05_002440"
     CDS             complement(4250..5602)
                     /gene="gorA"
                     /locus_tag="JQR05_002440"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY5324595.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEDPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5674..6516)
                     /locus_tag="JQR05_002441"
     CDS             complement(5674..6516)
                     /locus_tag="JQR05_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY5324596.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6719..8761
                     /gene="prlC"
                     /locus_tag="JQR05_002442"
     CDS             6719..8761
                     /gene="prlC"
                     /locus_tag="JQR05_002442"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY5324597.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8769..9521
                     /gene="rsmJ"
                     /locus_tag="JQR05_002443"
     CDS             8769..9521
                     /gene="rsmJ"
                     /locus_tag="JQR05_002443"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY5324598.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9570..11039)
                     /gene="dtpB"
                     /locus_tag="JQR05_002444"
     CDS             complement(9570..11039)
                     /gene="dtpB"
                     /locus_tag="JQR05_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY5324599.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQSVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11357..11791)
                     /gene="uspA"
                     /locus_tag="JQR05_002445"
     CDS             complement(11357..11791)
                     /gene="uspA"
                     /locus_tag="JQR05_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY5324600.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12182..12517
                     /gene="uspB"
                     /locus_tag="JQR05_002446"
     CDS             12182..12517
                     /gene="uspB"
                     /locus_tag="JQR05_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY5324601.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12588..14087)
                     /gene="pitA"
                     /locus_tag="JQR05_002447"
     CDS             complement(12588..14087)
                     /gene="pitA"
                     /locus_tag="JQR05_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY5324602.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14319..15518
                     /locus_tag="JQR05_002448"
     CDS             14319..15518
                     /locus_tag="JQR05_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY5324603.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKIPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
     gene            16684..18195
                     /locus_tag="JQR05_002449"
     CDS             16684..18195
                     /locus_tag="JQR05_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY5324604.1"
                     /translation="MPLQQFSRLALTLAIGLSIAAGGAALTWYQVAETDLSRTAAAAH
                     EAVSVTERLLDEAQGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLS
                     CSSWEGADPVREFISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVA
                     DNMTLLNLRRPLSLRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPL
                     TLYFHDGALSLLLSVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDA
                     STGEIAGVEVLARWVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGD
                     GKRWHISFNITQSHIMETGFIAECLTFIDAFTPGSITLTIELTEREPFDNSDEMRCQL
                     KRIHDAGIALALDDFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIE
                     MANTLKKEIIAEGVETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT         4764 a         4631 c         4842 g         4571 t
ORIGIN      
        1 aataaagcca taaataaaag atcctattaa tactcctaac aaaagatatg gcagtacatc
       61 tttaaactgt tgcaacgcat ctttggtggc tagttttatg ggattaatac ttactacagc
      121 tttggtactg caacaggtaa ctacggggtt ctcaatggct ttagccgctg gactacaaca
      181 actcactttt atgggcgtat atgtcgttcc cggcgaagtt tttgctggag cacaacaatt
      241 tgctgatgag cttttactgg caatgatatg acgttcaaac cccagagaat ctaggataat
      301 actggcaaga acggagacgc cggctgcgat aatcgcgtac aacaaggtga ctttccaacc
      361 aaaggtcatc cacattagtc cgacgataat gggattaagt aatggtgaaa caaataaaaa
      421 agtgagagtc ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca tgggtattgt
      481 cgagcaacta cagaacgggg taacggctcc caacagagca gccaggagat agcctctccc
      541 ttttctggcc cccatcatct gctggatttt atggtctggc accttttgtc ttatcaggct
      601 gacaccggca ctaatcacta taaacagcaa cgaaagctcg acggcgagaa acacaaacat
      661 ctctgcggca tcttgcagca tcgcaagcca actactcata cgtaacctca ttggataaat
      721 ctatatttcc agaataatcg aaataaaatt cagatcaaga tatttctggt attatcgaaa
      781 tatcaaccag acagaggggt gtcatatgca gttggaagaa gtcgctaaag cgctgaaaga
      841 gctaggtcac ccgacccgtt tgttcatatt taaacatctt gtcaaagccg gggaacaagg
      901 gttgccggtc ggtgagttgc aaaagcagct tgggatacct ggctccacac tcagccacca
      961 tatatcagca ctggtatcgg taggactggt caaacaaaac cgtgagagtc ggacgctgat
     1021 gtgtgtttcg caatacgcaa tgctggaagc gataattgaa ttcttacgcg aggagtgctg
     1081 cgtaaatagc aaaattgcct gatgcgctac gctctacata tttcatgcca tatattgaat
     1141 ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg catccgacat aaacaaagcg
     1201 cactttacca actatcggca gtggatatcg ccgctttatt tcaggcgctt acccgcttca
     1261 tcaaccactt tctcgccatc ttccttggag aatgcgcctt tttgcgcatc tggcagaatt
     1321 tccagcacca cttctgaagg gcggcacagg cgagttccca gcggcgtcac cacaatcggg
     1381 cgattaatca gaatcgggtg ctgaagcata aagtcgatta accgatcgtc agtaaattta
     1441 tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt tacgcagcag cgcgcgtacg
     1501 gaaatcccca tatcggcaat gagtttgacc agttcatcgc gcgttggcgg agtttccaga
     1561 taatggataa tggtcggttc tgtgccgctg ttgcggatca tctccagcgt attacgcgac
     1621 gtgccgcagg ccgggttgtg ataaatggta atgttgctca tatcagtatc tcattacaaa
     1681 gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa acagcacagg cagggtcatg
     1741 ataatccctg tacggaagta atatccccag ctgatagtca tattcttctg cgaaagtacg
     1801 tgcagccaga gcagcgttgc caggctacca atcggggtaa ttttcggccc caaatcgcag
     1861 ccaatcacgt tggcataaac catcgcttct ttgataacgc cagatgccgt gctgccatca
     1921 atggacaacg cgccaaccag taccgtcggc atattgttca taatagaaga gaggaaggcg
     1981 gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt tatccgccag cacgttgagt
     2041 acgccagaaa gatattccgt taatccggca ttgcgcaggc cataaaccac cagatacatg
     2101 ccgagcgaga agatgacaat ctgccagggg gcaccgcgca ggactttacc cgtattaatc
     2161 gcatgaccgc gtttagcgac gacaaataat atcagtgcgc ccacagctgc aatggcgctc
     2221 accggaatgc cgagcggttc caggacgaaa aatcccacca gcagaagcag taaaacaacc
     2281 cagccagttt tgaacgtagc aggatctttg atcgcttctg cgggagattt cagcagcgcc
     2341 atatcgtagt tctgcggaat atctttgcga aaatagagat gtagcatcac cagcgtggca
     2401 acaatcgcgg cgatatccac cggcaccatc accgaggcgt attcgcgaaa gccgaggcca
     2461 aagaaatcag cggaaacgat attcaccagg ttggagacaa taagcggcag gctggcggta
     2521 tcggcaatga atccggccgc catcacgaac gccagcgtcg tgcctttact gaatcctaaa
     2581 gccagcagca tggcgatgac aatcggcgtc aaaataagcg ccgcgccatc attggcaaac
     2641 agggccgcaa cggcggcacc gagcaggaca atccaggtaa acagcaagcg accgcgtcca
     2701 ttgccccagc gtgaaacgtg cagcgctgcc cattcaaaaa agccggactc atccagcagc
     2761 aggctgatga taatgacggc aataaacgcc gccgtcgcgt tccagacgat attccacacc
     2821 accggaatat cacccggatg aaccacgccc gtaaccaacg ccagtaccgc gccgagcgtt
     2881 gcactccagc cgatgcctaa acctttcggc tgccagataa ccaatacgat ggtcaggaca
     2941 aagatagcgc ctgccagtaa cataatgcct cccggataaa acacatgtgc aaatccatat
     3001 gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc tccggaacag ttttgtctgg
     3061 ccaggttgcg gacaattgcc tgaatctttt cctgttcaca tcgccaggct tcatcaataa
     3121 ttttcgccgc ccatgctgga atatgcggcg ataagcggta atgaacccac ttaccttgct
     3181 tgcggtccag caatagcccg ctttcacgca gcaatgccag atggcgggat atcttgggct
     3241 gcgactgatc gagggcagtg cagagatcgc agacgcataa ctctcccagt tcgctaagca
     3301 gtaaaacgat gcccagacgg gtttcatcag caagaatttt gaacaattgg atgggtaaca
     3361 gaaatgacat attgcgctcc tgattgttgc aggtagtgtc tctcttcgaa gcggataagt
     3421 caaaaacata tatgacttaa cgaatgtgta agtgcagagg aaggtaatag gtgtgaattt
     3481 tgagttggct attcatttga aaggcggttt gaatcaggac gtaaaaaaac cgactttgcg
     3541 tcggtttttt actcttcagc ctgagttggt ggctctggct ggagtgagaa tgcccacatc
     3601 taacggttca atactattag ccagtggcta acattgcaag cgttagcgat ttcttcagct
     3661 ggagaaggga cagtttgcta tacatggtgg tgaaagagga tttatatact ttcatcgggt
     3721 cgctgccgta ccaagccttg tccgaatccg gacgtaaaaa aaccgacttc gcgtcggttt
     3781 ttttactctt cagcccagag ttggtggctc tggctggagt gagaatgcca tcatctaacg
     3841 catcaatgct aataccatac tgaaactatt gcaaggttat gctggttatt tagcctaatt
     3901 acactgtatg attatccagt tagctctgag gcattttcac tctggcaatg cgcataaacg
     3961 ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa ctgatgctct ggccggagtg
     4021 agattgccca catctaacaa tgaggcatgc gccgtgacag gcagtggatg agtaagcgga
     4081 tgcattctca ctccatcgca tggagaaaac gggtgattga taaagcaatc atcgttcttg
     4141 gggcgttaat tgcgctgctg gaactgatcc gctttctgct tcagcttctg aactgatagc
     4201 ggaaacgtaa ttaagggcta agagcacact actcttagcc ctttaacatt taacgcatgg
     4261 tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt attgtcgaag tcttttttgg
     4321 ttgcgcccat cttcagcgcc accgcgaagc cctgcaacat ttcgtccata ccaaagccaa
     4381 tgccgtgaat accgacaatc ttctcttctg gcccaacgca caccagcttc atgcggcacg
     4441 gctggcggtg agtggtgacg gcggtataca tcgcggtgaa agaggattta tacactttca
     4501 cctgatcgtc gccgtactgc tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg
     4561 gcggatggct gaagaccacg gtcggaatgt tgctgtaatc cagatgctca tccggcttgt
     4621 tattaaacag gcgttcagag agacggcgac ccgctgcaac tgccaccggt gtcagttcca
     4681 ctgcacccgt gttatcgccc accgcgtaaa taccttcaat attggtgttt tgatatttat
     4741 cgacgacgat atagcctttt tcgttggttt taacgccagc ggcttcgagg ttgatgttgt
     4801 cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat
     4861 cttccagctc cagcgtcagg ctaccatcgg catttttcac taccgctttc gggatggcgt
     4921 tggtatgcaa ctgtgggtct tcggcgttca tcacttcgac cagcgtttca gagatcatcg
     4981 ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag atgcgttttc gcgccgaggc
     5041 cgttaatcac gccagccagc tcaacggcga tgtaacccgc gccaacaacc gccacgcgct
     5101 ctggcaaagc aggaagggcg aagaagccat cggaatcaat accgtattcc acgcctggaa
     5161 tatccgggtg gctcggacga ccgccggtgg cgatcagaat atgatcggcc gtgatggttt
     5221 cgccgttaac ttccagcgtt ttggcatcaa cgaagcgggc aaagcctttg attacatcaa
     5281 cgttattttt accgagcacg ttttcatagg aagtatgaat acggtcgata taggcggtac
     5341 ggctggcgat caacgtttcc cagttgaact tgttgatggt ggtatcgaaa ccataatcag
     5401 ggccgtacat atggatcgct tcacggattt gcgccgcgtg ccacatcact tttttcggca
     5461 cgcagccgac gttcacgcag gtgccgccca gctctttggc ttcaatcagt gcgcatttct
     5521 ggccgtacat agccgcgcgg ttgatggagg cgataccgcc gctgccgccg ccgatggcga
     5581 tgtaatcata gtgtttagtc atgacatatt gtccttatcg ttgattaccg cgattgtagc
     5641 gcgagacgta ataggtgcca gcaatggctg caattactcc ggcacgatcc agcttacggt
     5701 ggcgtgcccg gtacctgccg gaaccagttt gctgtgcagc cacgacagca cgttattcat
     5761 ctgctgttcc agcttccacg gcgggttaat cacaatcatg ccggaagcgg tcatgccacg
     5821 gcgatcgctg tctggcagca ccgccagttc aatttgcaga attttgcgaa tgccggtcgc
     5881 ttccaggtcg tggatcatgc gcttaatttg ctggcgcagc accaccggat accacaatgc
     5941 gtaagtgcca gtggcgaaac gtttgtaacc ttctgctatc ccgctgacca ccgcctgata
     6001 gtcggttttc atttcatacg gcgggtcgat aaggattaaa ccacggcgag aaaccggcgg
     6061 cagtttggcc ttaagttgct ggaaaccgtc ggctttttcg acgcgtgcac ggctgtcttt
     6121 ctgaaatcca gaacgcagca acgggtaatc gctcgggtgc agttcggtca gttgcaggct
     6181 gtcttgttcg cgcagtagct ggcgagcaat caacggcgaa ccggggtagt aacgcaactg
     6241 gccgctacgg ttgaagtgtt ttaccacatt gatgtacgcc tccagttctg cgggcaaatc
     6301 gtcctgctgc cagatacggg cgataccttc gagatattcg ccggtacgct cggcatgttc
     6361 gctacctaac tgataacgcc cggcccctgc gtgggtgtcg agatagagaa acggtttatc
     6421 tttctctttc agcgactcga tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg
     6481 gttgccagcg tgaaagctgt ggcgataact gagcatgggt aaaggtgttc cggtaagtaa
     6541 aaattggccc gtattcgggg cgcacaaaag cctatcagga cagtataccg aaaagaggcc
     6601 gccgccgcga aagcgtaacg tttctcattg aaattcacta cacttaaccc catgctacac
     6661 acattatgta aagcgcctgt tgagcgcttc cttaacctct ttaaccagga ctgcgctaat
     6721 gacgaatccg ttactgactc cctttgaatt gcctccgttt tctaaaattc tcccggaaca
     6781 tgtcgttcca gccgtgacta aggcattgaa cgactgccgc gaaaatgtgg agcgcgtagt
     6841 agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag ccgttggcgg aagtggacga
     6901 tgttttgggg cgtatcttct ccccggtcag ccacctgaac tcggtgaaaa atagcccgga
     6961 actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtcg gaatacagca cctgggtagg
     7021 gcaacatgaa gggctgtata aagcgtaccg cgacctgcgc gatggcgatc attacgccac
     7081 gctgaacacg gcgcagaaga aagcggttga taacgccctg cgtgattttg aactctctgg
     7141 cataggtctg ccgaaagaga aacagcagcg ttacggcgaa attgctaccc gtctttctga
     7201 actgggcaac cagtacagca acaacgtcct cgatgcgacg atgggctgga ccaaactcgt
     7261 taccgacgaa gcggggctgg cggggatgcc agaaagcgcg ctggctgcgg caaaagccca
     7321 ggccgaagcg aaagagctgg aaggctacct gctgacgctg gatatcccaa gctacctgcc
     7381 ggtaatgacc tactgcgaca accaggctct gcgcgaagag atgtatcgcg cttacagcac
     7441 ccgcgcctcc gatcaaggcc cgaacgccgg taagtgggat aacagcaagg tgatggaaga
     7501 gatcctcgcg ctgcgtcacg aactggcgca actgctgggc tttgaaaact acgcctttaa
     7561 atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg ctggatttct taaccgatct
     7621 ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggag caactgcgtg ccttcgctaa
     7681 agccgaattt ggtgtcgatg agttgcagcc gtgggatatc gcttactaca gcgaaaaaca
     7741 gaaacagcac ctctacagca tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa
     7801 agcggttaac ggcctgtttg aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg
     7861 taaagatgtt gatgtctggc atccggatgt acgtttcttc gaactgtatg acgaaaataa
     7921 cgaactgcgc ggcagcttct acctcgatct gtatgcccgt gaaaacaagc gcggcggggc
     7981 gtggatggat ggctgcgtag gccagatgcg taaagctgat ggttctttgc aaaaaccggt
     8041 cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca
     8101 cgacgaagtg atcaccctgt tccacgagtt cggtcacggc ctgcaccata tgctgacccg
     8161 catcgaaacc gctggtgttt ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc
     8221 gagtcagttt atggaaaact ggtgctggga gccggaggcg ctggcgttta tctctggtca
     8281 ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa
     8341 ctaccaggcg gcgctgttta ttctgcgtca gctggagttc ggcctgtttg atttccgcct
     8401 tcatgccgag ttccgcccgg atcagggggc aaaaatcctc gaaactctgg cagaaatcaa
     8461 gaaactggtt gccgtggtgc catctccgtc ctggggccgt ttcccgcacg ctttcagcca
     8521 tattttcgcc ggtggttatg cggcaggtta ctacagctac ctgtgggctg acgtactggc
     8581 ggcagatgct ttctcgcgct ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc
     8641 gttcctcgac aacattctga gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg
     8701 cttccgtggt cgtgaaccgc agctggatgc gatgctggag cattacggca ttaagggctg
     8761 atcattcagt gaaaatctgc ttaattgatg aaacaggcgc cggagacggc gccttatctg
     8821 ttctggcggc ccgctggggg ctggagcacg atgaagacaa cctgatggcg ctggtgttaa
     8881 cgccggaaca tctggaattg cgcaagcgtg atgagccaaa acttggcggc atctttgttg
     8941 attttgttgg cggagcgatg gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg
     9001 tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga tgtggtggac gccaccgcag
     9061 ggctggggcg cgatgccttt gtactggctt cagtcggctg ccgcgtgcgg atgctggagc
     9121 gtaatccagt ggttgccgcg ctactcgacg acggcctggc gcgtggttat gcggatgcgg
     9181 aaatcggcgg ctggttgcag gagcggttgc agttaattca cgcctccagc ctgacggcgc
     9241 taaccgatat tacaccacgc ccgcaggtgg tctatctcga tccgatgttc ccgcataagc
     9301 agaaaagcgc gctggtgaaa aaagagatgc gtgtctttca gtcgctggtg gggccggatc
     9361 ttgatgccga tggattactg gagcctgcac gcttgctggc gaccaaacgc gtggtggtaa
     9421 agcgcccgga ctacgcgcca ccgctggcga atgtcgccac gccaaacgcg gttgtgacta
     9481 aagggcatcg ctttgatatc tatgcaggta cgccagtgta aaagtaaagc agggtagcga
     9541 taacgctacc ctgctttctg caagaataat taatggcttt ccggcgtcgc aatcatgcgt
     9601 ttcagccacg gcaccatcaa cagcatcact actgcaacgc ccagcgtgac cagaccaatc
     9661 ttaccaaaca cgttggtata gacgggcaac gtctcaagcg gatcggtaat gttgtccggc
     9721 accgcggtaa atgttgccac atagccgccc agcaagaacg cggcagcctg cgtcaggaac
     9781 cacatcccga gaataaagcc catcaaatgc tgcggcacca gggcagcaat catcgccagg
     9841 ccaagggcgc taataaacaa ttcacccagg ctctggaata aatacaccag cacgataaac
     9901 catggcgatg tcagcccctg ggcatccgca aaccacattc ccgcagcagc agccgtcaaa
     9961 aagcccagtg agcacataaa catgccgaga gtaaatttca tcggcatcga gaggtctttg
    10021 cctttgttac ccagatgcgt gtaaatgcct gccagtattg ggctggcgag taccacccag
    10081 aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa aaccgagaat ttcatgatgc
    10141 acgttgttga tggcaaagaa gttcagcgat gttggcatct gggcgtagag aatgtaaaac
    10201 accaccgctt cgagcatcag gacaaaggcg acaaacattt tattgcgccc ggttttatcc
    10261 agcttgaatg cctgacgaaa gaagatgatg gtgacgacga tggagagaac aatcagcacc
    10321 agattggcga cttctacgtt gtgcatcagc catgcacata cgaagatcat caccacgctg
    10381 ccaagtaata cgtacaacag tttgctgaag ctcatcggct tgaagtcggg ttcagaacca
    10441 atgtctttca ccattccacg acaggcgatg taaaccagta atgcgataat taaccccgcc
    10501 ccgcacaggt tgtaggtgac tgaataaccg aatctatcag cgatcacagg ggccagcgat
    10561 aacgctatca acgagccgat attgatcgac atatagaaca gggtgaatgc gccatcaagc
    10621 cgcggatctt tcggcggata gcacttcgaa agcaagctgg ctgggttagc tttaaacagg
    10681 ccgttaccga cagcgatagt ccctagggcg atgaaaatca ggtcaggctt aagtagcgac
    10741 atgccggtca tgaagtagcc aatcgccagc acaagtgctc caagaacaat ggtgcgtttg
    10801 gtccccaaca ggtggtcgcc gacatagccg ccaatggaaa tgaggccata gaccagtgca
    10861 gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga atccaagctg tttaacgaag
    10921 aaaaccgcca gtacgctctg cacgccgtag tagccgaatc gctcccataa ctcgacaaaa
    10981 aagatcatga aaaatgggcg aggttgctgc agcatcccca tgggtgttgt tgtattaata
    11041 tttattaacc ttccaatacc atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa
    11101 aagtttacat ctgtcagaga ttcttaatat tgatgcggtg aatagattaa tacactatta
    11161 caacagaaaa taaccagctg atcacactaa tgatggaaaa tatgatggtg ttaaattttg
    11221 tattggcagg agattttgat tgcgtacgca gatcgtaggc cagataaggg gtttacgctg
    11281 catcaggtaa caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca
    11341 cgtcgtggag ggatgattat tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt
    11401 gaacggtgtt gatcagctga cgtgcggaag acatcagttt gctccagaag tcctggtggt
    11461 gaccacaaac caccagatcc atatcgtatt tcttgattgc atcgaccaga acctggccca
    11521 ggtcgccgct gccgctcagg gtttcagtga ttggatagcc tgcattagtg gaaagctcgg
    11581 tcagcgcatg atgtgtctct tcagagatgc gtttctgcat atcacccaga ttcacatcaa
    11641 taagcccggt gtataggtca gagtagttta catctacgtg gatcagagaa actttcgcat
    11701 tgtaggggcg agccatagag actgctttct ctaccagaac tttgctttcc ggggagagat
    11761 cgactgcgat gagaatgtgt ttataagcca tagtgttact ccttccataa agttgtcgat
    11821 gactggccag ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg
    11881 cggggctaac ccagccgtaa agattcagtg ttaagaccat ccttacctta tagcgacccg
    11941 gatgatccgt caatccgcct tgcttacccg ttagataaac aaattttcga catgaatata
    12001 ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc
    12061 cgctcggatg ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc
    12121 agcgtatcgg gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg
    12181 tatgataagc accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc
    12241 gcgctatttc tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct
    12301 ctatcaatat gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt
    12361 gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat catcacgatg atgagtttat
    12421 tcgccgctgt gagcgggtgc gtcggcagtt tattctgact agcgcattgt gtggtctggt
    12481 ggtggtcagc ctgattgcat tgatgatttg gcattaagca cttcagcaat aataacgcat
    12541 aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg cgtacgatta caggaactgc
    12601 aaggagagcc agtacagccc gccggaaagt agtaccgcag ccggaagggt aaacacccag
    12661 gccatcagaa tactggttac ggttttacgc tgtaagccgc cgccatctac caccatcgtc
    12721 cccgcgacag aagaggagag tacgtgagtg gtggaaaccg gcatcccggt ataactcgcc
    12781 aggccgattg acactgccgc cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct
    12841 ttcttaccga ttttctcacc gatagtcgtt gccacacgac gccagccaat catcgtaccg
    12901 atacctaacg ccagcgcgac cgccatgatg atccacaccg gcgcatactc gatggtgcta
    12961 agcatgtcgg acttcagttt cttcaacagg cgctgatcgt cagcactcac accaggcatc
    13021 ttcaccactt tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt
    13081 tgatcaagcg acagcttgtc gtagctttcc acatcggtgg tcaacatacc tttcaggcgg
    13141 ttgagcgcgt taatggtatt cgacggatgg cagtggaact ccgcaggttg cgttgcgcca
    13201 gcttccggag ccggtactaa ctgatcagca ccggtagcct gtttgagcag cgcaggatgc
    13261 tgctcaaagt aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca
    13321 gtggcattca tgttcaccac gaagcctgct ggcgcgacgc caatcaatac caacataacc
    13381 agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc gatagcggaa
    13441 aggatcagcg cgatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc
    13501 tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc
    13561 aagaaaatca gaccgccagc aaacaccagg ccgacaatag gggaaacgat cagagaaccg
    13621 aaaatactta atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac
    13681 gcattggtta aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt
    13741 aaaccaaagt accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc
    13801 atggcaaggc catgagacga tcccatatta agcagcagat ccgtcggcag catatgcaca
    13861 atggcatagg caacactcag accacccagc aaaacaccca aaaagttgaa caccgccgcc
    13921 ataaccacgg cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg
    13981 gctgtgtcat ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat
    14041 aataacagcc cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc
    14101 attttgagga catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa
    14161 tatagacttt ttttgatttg cctcctgtat ggatttcact caaaaaataa ttatcttata
    14221 taattcaggc aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct
    14281 ttacaattca cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt gatgccatta
    14341 ttataggcgc gggtgcggcg ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc
    14401 gggttctgct gatcgataat ggtaaaatac cagggcgcaa aatccttatg tctggcggtg
    14461 ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc
    14521 atttttgtaa gtctgcactc gcacgtttta cccagtggga tttcattgat ctggtcaata
    14581 aacacggtat cgcctggcac gagaaaacgt tagggcaact cttctgcgat gactccgcgc
    14641 agcagattgt cgacatgctg gtggatgagt gcgagaaggg caatgtaacc ttcagattgc
    14701 gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt cacgcttgat ctgaacggca
    14761 tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg gctgtcaatg ccggggctgg
    14821 gcgcatcgcc gtttggttat aagattgccg aacaatttgg cctcaacgtg ctgccgaccc
    14881 gcgcgggtct ggtgccattc actctgcata aaccgttgct cgaagagtta caggtgctgg
    14941 cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg caccgttttc cgtgagaact
    15001 tactcttcac ccaccgcggc ttatctggac cggcggtgtt gcagatttca agctactggc
    15061 aaccgggtga atttgtcagt atcaatctgc taccggatgt ggacctcgaa accttcctga
    15121 atgagcagcg taacgcacat ccgaatcaaa gcctgaaaaa cacactggcg gttcatctac
    15181 cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt tcgctaaaac
    15241 agctcaacgt gtgtgaccaa caggcactga ttagcacatt gaccgactgg cgcgtacaac
    15301 ccaacggcac tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg
    15361 aactttcttc acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag
    15421 tgatggacgt taccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcat
    15481 gggcttgtgc gcaggatttg gcgtcaattg tgaaataacc atgacgtttt cccgtcagcc
    15541 gtttgtatat gtcgttatta agtcattttg gtacggttct gcatccgaca ttctggtgtt
    15601 aactcgctgt tttgttagcg aattaacgct attgccgttg tggttatttt atagtgtatt
    15661 tattctcaga gaatatatat tactgaaata aaaaacacaa agaataacca aaggaaatta
    15721 tttgaaacgt aaaaggtata tatttttaat ctgtaaaatt taaaatcacc ccctctggat
    15781 tgaatcaact tgctctattt aagacgattg tttttacatt ttcaccacct gtatcaggtg
    15841 gtgttttttt attggcggca atgcctttat cttattttta ttacgttgta aatatggcta
    15901 ttcctaaatg aaatttgatt tgctcagttt atttgtgatg tcaggggagt gcttgccatg
    15961 agagtcgtga tattattatt catcattttt ataccgagtg tggcggatgc tttttcattc
    16021 gatagttcaa taattaatct caaatgtcct aggcgtggaa ctatcgaaat aatattacac
    16081 agatacgaac acacccagga aagctgggga ggggagaatt ttgaaaccgg gagtgggtat
    16141 agtcatgagg gcgcgttatt tatttttgac tttgccaatc aggataaaat gatttacaat
    16201 cgcgcggaga attcgtttgc tttttggtat gaatcttccc ggaaattagt taactgtaac
    16261 ttaataacgc ttaaaagcac atatcctgtc aacctgcctt atgttcatgg ctgatgtgat
    16321 gtgtgtcggg aactaacatc ttcatctttg tatatgacat tacgataatg ttaagctggt
    16381 ctttgtcata aatttaccct tagagatact actcccttct gtcaatatta ttcaagccga
    16441 acgttatact ggcttttgtc ggtcacggat atcttttttt cagtgagaat tcaaagtgag
    16501 ggtatataag aagtcgtatg agtaagtatc tttgttgtgg tgggttatta ttttatgggc
    16561 aaaatattaa gcagcgaaat cttttttttg cccagacgtt ttttgtagaa aaaagaattt
    16621 attagtccgg ctgaaaagca agaactgcta taactaatta agcgtaacgg aacggtacaa
    16681 aacatgccct tacaacaatt ttcccgatta gcgctcactc tggcgatcgg cctttcgatt
    16741 gctgcgggtg gggccgcgct gacctggtat caggtcgctg aaacagacct gagcagaaca
    16801 gccgctgctg cccatgaggc ggtctcggtt actgaacgac tgctggacga agcccagggc
    16861 gcaatcgatc tggtttatcc cctgagtgtg caaccatgta ccccacagct cagaaccgaa
    16921 cttggccggt tgaccattag tcacgaacat atccgggtga ttaacatttt taatcagaat
    16981 cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc gggagttcat ctccgctggc
    17041 gacaaccaga cgctgattct ggcgacagac gattatatat cacctggtgt gccggtcatg
    17101 attctacgca aaacggataa tgacaacgcc ataactgcga gtatcgccac taaatgggtg
    17161 gctgacaata tgacacttct gaatcttcgt aggccgctgt cgctgcgcgt agggaatact
    17221 gttcttacca gcgataacac gctggtgaaa gcgatacctg gagggaatag acacgcagtt
    17281 tattctggcc tgtatccctt ttccatagaa tatcctggcg atctttccgt tcctctaacg
    17341 ctttattttc atgatggtgc gttatcttta ctgctttctg tgcttctggg ggccgctgtg
    17401 gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc gctatgatga aatctacgag
    17461 gcaatgagga aagatgagat tgtgccgtgg tatcagccaa ttatggatgc ctcaacaggt
    17521 gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag cagatgggga agtcatttat
    17581 cccggcagtt ttatcactga agtagaacgt tcagatttag ttattccatt gactcgtagc
    17641 ctgatggcgc gggcatcccg ggatttaccg cctctgacag gcgacggaaa gcgttggcat
    17701 attagtttca atattacgca gtcacacatt atggaaacgg gatttattgc cgagtgcctg
    17761 acgtttattg atgcttttac accgggcagt atcactctca ctatagagct cacagagcga
    17821 gaaccctttg ataacagcga tgagatgcga tgccagctta agcgtattca tgatgcaggc
    17881 attgcccttg cgctggacga ctttggcacc ggttacgcaa atatggaata tcttagtgag
    17941 atcccagtcg atatcatcaa aattgatcgc gtctttgtga acagaatagg tcagggcgaa
    18001 tctgcagaac acctgctgac cagccttatc gagatggcca atacgctgaa gaaagagatt
    18061 atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc tatatgagcg tggtgttggc
    18121 aagttacaag gattcctata ttcaccggca ttaccgcccg ataagttggc actgtttaaa
    18181 ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat cattgaaccg gtgagggccg
    18241 ccccctttat tactcagggg gggctttcaa cgttctgaat taagatgttt tatgactcac
    18301 tgagcaggat gtgagttcca acgtaatctc cgctattttt actgtttttc atttgctgcc
    18361 tcatctatcc cttcgccttg cctctgagtt gcgttaaagg ctgatgtact gcctttaacc
    18421 cgttcaatac atagcctttt tgtcaatcat catcccgaag agcgttgcac tgtgccttcc
    18481 atagctgtgg ttgtcctctc aatctgtcag cccatttatg gaaaatctta acgaagcaac
    18541 aatgagatat gtaaaaaaag ggccgattta atcattcatt aagacaggtc attttttctt
    18601 actgtgaggg cggtaagctt ttctgtcggt aacaggttgg gagatgctgg tattatgaga
    18661 tttttctacc gtcctgcggc ggaatatgtg aaaaatagaa atgattatta ttattgcatg
    18721 gttattttta gtttgtgatg gtggagaata atcctattcc cgtgaccttg ttttttacat
    18781 tttatttaac tgcatggtat atgcattt
//
LOCUS       DADKXM010000072        18780 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.072, whole genome shotgun sequence.
ACCESSION   DADKXM010000072 DADKXM010000000
VERSION     DADKXM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.072"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..913)
                     /locus_tag="JQR05_002450"
                     /pseudo
     CDS             complement(1..913)
                     /locus_tag="JQR05_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1185..1279)
                     /locus_tag="JQR05_002451"
     tRNA            complement(1185..1279)
                     /locus_tag="JQR05_002451"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Sec,seq:tca)
     gene            1572..2955
                     /locus_tag="JQR05_002452"
                     /pseudo
     CDS             1572..2955
                     /locus_tag="JQR05_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            2965..5283
                     /gene="yicI"
                     /locus_tag="JQR05_002453"
     CDS             2965..5283
                     /gene="yicI"
                     /locus_tag="JQR05_002453"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY5324605.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5336..7045)
                     /locus_tag="JQR05_002454"
     CDS             complement(5336..7045)
                     /locus_tag="JQR05_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY5324606.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTSLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(7166..8557)
                     /gene="xanP"
                     /locus_tag="JQR05_002455"
     CDS             complement(7166..8557)
                     /gene="xanP"
                     /locus_tag="JQR05_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY5324607.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNKPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8837..10042
                     /gene="gltS"
                     /locus_tag="JQR05_002456"
     CDS             8837..10042
                     /gene="gltS"
                     /locus_tag="JQR05_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY5324608.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(10076..12157)
                     /gene="recG"
                     /locus_tag="JQR05_002457"
     CDS             complement(10076..12157)
                     /gene="recG"
                     /locus_tag="JQR05_002457"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY5324609.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTRQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12163..12852)
                     /gene="trmH"
                     /locus_tag="JQR05_002458"
     CDS             complement(12163..12852)
                     /gene="trmH"
                     /locus_tag="JQR05_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY5324610.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAAQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12859..14967)
                     /gene="spoT"
                     /locus_tag="JQR05_002459"
     CDS             complement(12859..14967)
                     /gene="spoT"
                     /locus_tag="JQR05_002459"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY5324611.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14986..15261)
                     /gene="rpoZ"
                     /locus_tag="JQR05_002460"
     CDS             complement(14986..15261)
                     /gene="rpoZ"
                     /locus_tag="JQR05_002460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY5324612.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15316..15939)
                     /gene="gmk"
                     /locus_tag="JQR05_002461"
     CDS             complement(15316..15939)
                     /gene="gmk"
                     /locus_tag="JQR05_002461"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY5324613.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16197..17879
                     /gene="ligB"
                     /locus_tag="JQR05_002462"
     CDS             16197..17879
                     /gene="ligB"
                     /locus_tag="JQR05_002462"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY5324614.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17876..18493)
                     /locus_tag="JQR05_002463"
     CDS             complement(17876..18493)
                     /locus_tag="JQR05_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY5324615.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
BASE COUNT         4321 a         4741 c         5064 g         4654 t
ORIGIN      
        1 atgatgtttt gagattggtt caagctgctt aaatagcata atgacctgct cagagagcgg
       61 cacaatatga ggtcgcttca tcttcattac ttctgccggg atctcccaca attttgtctc
      121 aaaatcgata tcttcccagc gtgcaaaacg cagttcttgt gttcgtacgc cggttaacat
      181 aatgatctga gtagctgtct tagtgattat acttccggta taacccgcca gatcgttgag
      241 gaagtgggga agttcattag cagtaagaaa gggaaaatgt acttttttcg gtgtagctaa
      301 agcactggca agatcggggg ctgggttata gtctgcccgg ccagtaacaa ttgcatagcg
      361 gaacacctca ccacagcgct ggcggacctt gcgcattttc tccagagctc cacgtttttc
      421 cattttacgc agtgcttcca gtaactccat tggtttgatt tcggcaatag ggcgcttgcc
      481 aatgtaggga aaaatgtctt tttcaaaggt atcaatgatt tcatcacgat agcgtaatga
      541 ccaccgatca gcttttaact ggtgccattc gcgagcaata gcctcgaacg tatttttcag
      601 actcatttgc tgcgcgattt tttcttcctt ttttgcctcc cctggatcgc ctccagcagc
      661 gaggagtttc ctggcttcac tgcgtttctc gcgagcatcg gctagcgtta catcggggta
      721 tacgccaata gaaagcatct tttctttacc agcaaagcgg tacttcatgc gccagtattt
      781 agaaccatta gtgttaatca gtagatacat accgccgcca tcagcaagct tatagggttt
      841 gtctttgggt tttgcggttt tcactttgat atcggttaat gccatgacat cctccggctt
      901 ttgatgaatt catgttcctg agggggattt ctgccagtaa ctaacctgat acccccaata
      961 caccccttgt ataacaaaga tattgttaaa agccgttgtt atgtatcaca tttttcctat
     1021 tatgggggtg cttgtatttc aataggaaat aacaccccct aaagtacccc caaaagaaac
     1081 ttgatacggg gtgaagtagg ttgacctcag gatacaaata aatactgata tacaagggat
     1141 tagaagagat ttattgacat tgggagagtt cagaagaggt aaactggcgg aagatcacag
     1201 gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca
     1261 ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta
     1321 cttccttgag tttctacatc ccccagatca gttttttccg cctaataaac gcagcgtatt
     1381 atcacgaaag cattataatt catataaaac ccatatttta caatgatata tgagcggaat
     1441 taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat
     1501 tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca
     1561 aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg
     1621 gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata
     1681 ccgatatttt tggcattcct gccggatttg tcggaaccat gtttttggtc gctcgtgcac
     1741 tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg
     1801 gtaaatttcg tccgtgggta ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg
     1861 cctatagcac gccagatctc agtatgaacg gcaaaatgat ctatgcagca attacttaca
     1921 ccctaactta ccttacttta taccgtcgtc aatatccctt actgcgcatt gggtggtgta
     1981 atcaccaatg acccgactca gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc
     2041 gcgggaggca tgctttctac tgttctgatg atgccactgg ttaatttaat tggcggtgat
     2101 aataaaccac tcggtttcca gggcggtatc gtggtccttt ccgtggtggc attcatgatg
     2161 ctggcatttt gtttcttcac cactaaagaa cgcgttgaag caccacctac aacaacgtct
     2221 atgcgggaag atttacgtga tatctggcaa aacgaccagt ggcggattgt cggtttacta
     2281 accattttca atatcctagc ggtgtgcgta cgcggtgggg cgatgatgta ttacgtcaca
     2341 tggattttgg gcacgccgga agtgtttgtc gcttttctca ccacttattg cgtgggtaac
     2401 ctgattggtt ccgcactggc aaaacctctg accgactgga aatgtaaagt cactatcttc
     2461 tggtggacga acgccctgct ggcagtgatt agcctcgcga tgttctttgt tcccatgcag
     2521 gccagcatca ctatgtttgt cttcatcttc gtgattggtg tgttgcatca actggtgaca
     2581 cctatccagt gggtaatgat gtccgatacc gtcgactacg gcgagtggtg caatggtaaa
     2641 cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc tcaaactggg gttggccttc
     2701 ggcggcgctc ttatcggctg gatgctggct tatggcggat atgatgcggc agaaaaagcg
     2761 cagaacagcg ccacgattag catcattatt gcgctattca cgattgttcc ggcgatctgt
     2821 tatttgctga gcgcgattat cgctaaacgc tactactcac tcacgacgca caatctgaaa
     2881 accgttatgg aacagctggc tcagggtaaa cgccgttgcc agcaacaatt cacctctcaa
     2941 gaagtgcaga actaaggaac ggcaatgaaa attagcgatg gaaactggtt gattcaacct
     3001 ggcctcaatt tgattcaccc gcttcaggta ttcgaggttg aacagcagga taatgaaatg
     3061 gtggtctatg ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga tacgccttta
     3121 tttacgctgc gctttttctc cccacaggaa ggtattgtcg gtgtgcggat tgagcatttt
     3181 cagggggcgc tgaataacgg tcctcattat ccgctcaata ttttgcagga tgtgaaggtc
     3241 acaatcgaaa acacagaacg ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc
     3301 agcaaaggtg agttctggtc actggatttt ctgcgcaacg gcgaacgtat taccggtagt
     3361 caggtgaaaa ataatggcta cgtgcaggac acgaataatc aacgcaatta tatgtttgag
     3421 cggcttgatc ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt tactgccctg
     3481 gtgcgcaatg gccagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag
     3541 gcgtataaaa atatcccgtt ctacatgact aaccgtggtt atggggtact ggtcaatcat
     3601 ccccagtgtg tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt
     3661 gagagtgaat atctcgaata ctttgttatc gacggcccga cgccgaaagc ggtacttgat
     3721 cgttataccc gctttactgg tcgtccggcg ctgccgcccc cgtggtcctt cggcctgtgg
     3781 ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt
     3841 atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc
     3901 ttccagtggt gcgattttga gtgggacccg ctgactttcc ctgacccgga agggatgatc
     3961 cgccgcctga aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa
     4021 aaatcccccg tctttaaaga gttacaagag aaaggctatt tactcaaacg cccggacggt
     4081 tcgctatggc agtgggataa atggcagcca ggtctggcga tttatgactt taccaatccg
     4141 gatgcctgca aatggtacgc cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc
     4201 tttaagaccg actttggcga acgtatccca actgatgttc agtggtttga cggttccgat
     4261 ccgcagaaaa tgcataacca ttatgcgtac atctacaacg aactggtgtg gaacgtgctc
     4321 aaggacaccg ttggtgagga agaagctgtc ttgtttgccc gctcggcctc cgtcggtgcg
     4381 cagaaattcc cggtacactg gggtggcgat tgttacgcta actacgaatc aatggcggaa
     4441 agcctgcgcg gtggtttgtc tattggcctt tcaggttttg gcttctggag ccacgatatc
     4501 ggcggctttg aaaataccgc tccggcgcac gtttacaaac gctggtgcgc gtttggtttg
     4561 ctctccagcc atagccgttt acacggtagc aaatcttatc gtgtgccgtg ggcctacgat
     4621 gatgagtcct gtgatgtggt gcgcttcttc acgcaactga aatgccgcat gatgccgtat
     4681 ctgtatcgtg aagctgcgcg tgcgaacgcg cggggtacgc cgatgatgcg ggccatgatg
     4741 atggagttcc cggacgatcc ggcttgtgat taccttgacc gtcaatacat gttaggcgac
     4801 aacgtgatgg ttgcgccggt gttcactgaa gcgggcgatg tgcagttcta cctgccggaa
     4861 ggtcgctgga cacacctgtg gcacaacgat gaactcgacg gtagtcgctg gcataaacag
     4921 cagcacggct tcctgagtct gcccgtttat gtgcgtgata acactctact ggcgctgggc
     4981 aacaacgatc aacgtcccga ttacgtgtgg cacgaaggca cggcattcca cctctttaat
     5041 ctgcaagacg ggcatgaagc cgtctgtgaa gtgcccgctg ctgacggatc ggtgatcttt
     5101 actttaaaag cagcacgtac tggcaacacg attactgtga ctggtgcggg cgaggcgaag
     5161 aactggacgc tgtgcttgcg caatgttgtg aaagtaaatg gtctgcaagg cggttcgcag
     5221 gctgaaagtg agcaggggct ggtggtgacg cctcaaggga atgcgctgac aattacgttg
     5281 taatctattg acggaatgcc gggtacgtct gagtcgtacc cggcgagatt gatgtttacc
     5341 gcaccggctc cgtcgatgta acttcaccgc tggaaacgac gcccgcattc atggtttgag
     5401 tcacttgctg ctttgcggcg ttcacggcat gcaattgccc gctaaccgta ggtttcagcg
     5461 gggcattggc ctgaatatct ccactggcgg taagctgaat attgccgtcg ccagtcaacg
     5521 gtaattcagg ccatccccat tgttgcaaaa tattcaccgg cacaccgcgt ccattcaggc
     5581 tgatatgggt ctgacgttgt ggcgtttgtg aaacacttgc ggtggcttcc agaatgcctt
     5641 tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct gctgttggcg gtcagcgcca
     5701 gcgacgggcg acgaacatca acacgattga atgtcgcggc ggcggcattc agattcgccg
     5761 agccactcca gacgccccat ttatgatcgg taaccagcgt caggttggca ccgtaaccat
     5821 cgagcgaggt gagctgccac gggaagtcag ggtcgatatc aatgatcaga ttgcggctgg
     5881 cgctaaatct cttcagttgc aggctgttta accaaccggg tgtcgtttcc atccacaact
     5941 gttgccagtt tttcggcaag gtatattcca gcccggcaat tgccgcatca tcaaggatca
     6001 acgttttacc gtcacgcagc cagttccctg acgttctgac cataccccct tcccagcggc
     6061 tggtgaactg gcgcagcgct acgccctgcg gggaaaattc cgcgtttata atcgggtcaa
     6121 ataaatgcag cgaaccataa atgaactcgc tggcgttcat cgacagtttg ccttcctgtg
     6181 tctgccagtc atctttactg aaggtcatgt tgcgcaagct gagatcgagg tcggtcaccg
     6241 cccagtccgg accttgcaga cgagcatcga tgacttccaa gcgaccaatc tgcaacgacg
     6301 ggacagagcg taatggcgca aagaagtcgg ttagcgattt ttcgctttgt agacggatat
     6361 ccgccatgcg cagattttcc acttgccagc tgccgtcggc gttacgctgc gcgtttccgg
     6421 ttaatgtccc gcgggcgatg tcggcaccca ggttagtcag cgtaacgcga tcgttatcaa
     6481 tactgccttc aatcagtaca ttggtggcag gaacatcatt gagcgaaagc gatccggcac
     6541 taaactgaat ctgcgccttc gtacccagca ctttaccggc ttctggtgac cacggaacca
     6601 cgccgccatt tacccgctgc gcgctcagtt tccattcgct attcgggcta ttaaacgcca
     6661 tatcacgcag ttgcagacga tcggctttga acggtagcgg cgcggtctgg tcggtgagat
     6721 tcagcgtccc gttttccagc aggatggtat cgacatggcg tggttcggtc agttggcgac
     6781 tgcttagcgc aatgtcgaca ctttttgcca ccaggggcgc gggctgacca tcacgaccaa
     6841 acgtgacatt ctccagcacg atatgagatg gcgcggaaaa acggtgatcc atcgccccga
     6901 aggccagatg atagtcgcta ttctcggaaa cccatgcgct gatatgttct gctccccagc
     6961 gggtttgcag aagaaaatag aggccagcga tcaccactaa cagagcgatg agaatgtaga
     7021 gaagcagctt cccaataaat ttcatggtct tccatcccgt gaaatgcaca taagggagtt
     7081 atgcacgatt tacgcgcaat cctcaaggcg ggaatggtga aagaggtgag actgcggcgg
     7141 taattatctt accgccgcca gtgaattact gtttttctgg tgggaaaatc agattcagca
     7201 cgatggcagt aataccgccc gcggcgatcc cggaggagag caggtttttc agccattcag
     7261 gggcaaactg caaaatcagc ggctgctgag acacgcccag accaaccgcc agcgacagcg
     7321 cgataatcag aatcgcccga cggttcagcg gctcacgaga aacgatacgc acaccggagg
     7381 cggcgatggt gccaaacatt acaagcgttg cgccgcccag aaccggttct ggaatgtgtt
     7441 gtacaaaacc gctcactgcc gggaacagac ccagcacgat caacatcagc gcgacgacaa
     7501 aaccgacata gcggctggca acaccagtca actggatcac tccgttgttt tgcccgaagc
     7561 aggagttcgg gaaggtgtta aacaccgccg aaacaaacga gttcaggccg tttgccagca
     7621 cgccgccttt caggcgtttc atgtacagcg gaccggacac tggctgttcg gaaacgtcag
     7681 aggtcgccgt gatatcgcca atggtttcca gcgaagtgat cataaagacc agcatcagcg
     7741 gcagcagcag actccattca atgccaagac cgtaatagag cggcgttggc accataatca
     7801 gttcttgcgt catcggtttg ttgctttctg gcaacatgcc cataaaccac gccagcgcat
     7861 atccggccgc catcgcaatt accagtgagg ccacgcgtaa gtaagggtta cgttgacggt
     7921 taagcaggat aattaaggct aaaaccacgc ctgccagcag cagatttttc ggtgcgccga
     7981 aggtgttatc gctcatggct gcgtaaccgc cgccaatgga ggtcagcccc acctgaatta
     8041 gcgacaggcc gataatcatc accacaacgc cagaaaccag cggcgtaata atgcggcgcg
     8101 ccagatgcag aacgcgggag atcaccatct cggtgcaact tgccagcatc aacgtgccga
     8161 acaaagccgc catcatggta ggaacatcag caccaccggt tttcagcgcg gtaccgccca
     8221 taatcagcgg ggcaacaaag ttgaagctgg tgccctgaat agacaacagc ccggagccaa
     8281 ccggacccca ggccttaatt tgaataatcg atgccacacc ggaggcaaac agcgacatac
     8341 taataatgtg ttgcgtgtct tgtgccggta aacccagcgc ctggcagatt aatagcgctg
     8401 gcgtgatcac cgcaacgaac atcgccagca gatgctgaca ggcggcaaac agggtttgag
     8461 gaagcggcgg acgatcttca agacggtaaa tcagttcgct gttttgagtc tgcgcaaccg
     8521 gttgcgcatt ttctgactcg agggtggaaa cagacatcga aaacaatccc gtggtagcaa
     8581 agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat
     8641 atttaaatga gataaatgta cttttttata taacttttgg tatttttctg cttaaaatcc
     8701 atgccatttg ttcattaaca gctgagaatt tgacccagga aagcataact cgatacccgt
     8761 catagttcat gaagttgcca ctgcgcgatg aagtatgacg agtatgaaag agtgatgcgg
     8821 atacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg ccgcaacgct
     8881 gacgttgctg ctcgggcgta agttggtcca ttccgtctcc tttttgaaga aatacaccat
     8941 accggaacct gttgcgggtg gtttgttggt ggcgctggcg ctactagtac tgaaaaaaag
     9001 catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttaatgc tggctttctt
     9061 cgccaccatt ggcctgaacg ccaacattgc cagtttgcgt gccggtgggc gtgtggttgg
     9121 catcttcttg attgtggttg ttggtctgtt ggtgatgcaa aatgccattg gcattggtat
     9181 ggctagcctg ttagggcttg atccgctgat ggggctgttg gccggttcta ttacgctttc
     9241 cggcggtcac ggtacgggcg ctgcgtggag taaattgttc attgaacgtt atggcttcac
     9301 caatgcgacg gaagtggcga tggcctgtgc aacgttcggt ctggtgctgg gcggcttgat
     9361 tggcggtccg gtggcgcgct atctggtgaa acactccacc acgccgaacg gtattccgga
     9421 tgaccaggaa gtcccgacgg cgtttgaaaa gccggatgtg ggacgcatga tcacctcgtt
     9481 ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga aaattgttgc
     9541 gcaacttttg gctggcactg cttttgaact gccgaccttc gtctgtgtac tgtttgttgg
     9601 cgtgattctg agcaacggtc tgtcaataat gggcttttac cgcgtctttg agcgtgcggt
     9661 atccgtgctg ggtaacgtaa gcttgtcgtt gttcctggcg atggcgttga tggggctgaa
     9721 actgtgggag ctggcttcgc tggcgctgcc gatgctggcg attctggtgg tacagaccat
     9781 cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg atgggcaaaa actacgatgc
     9841 ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa cggcaatcgc
     9901 caacatgcag gcgatcactg aacgctttgg cccgtcgcac atggcgtttt tggtggtgcc
     9961 gatggtcggt gcgttcttta tcgatatcgt caatgcgctg gtgattaagc tgtatttaat
    10021 gttgccgatt tttgccggtt aaccgatgaa gcggcggtct tctcaccgcc gccttttacg
    10081 cattcgagta acgttctgtc tccggcatcc agcgttctat caaggctttt gcctgttgtg
    10141 ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc gcctgatcgc
    10201 gcagtaaatc cgccacttta aattcagcat tacccgtctg acgcgtgcct aacaattcgc
    10261 cagggccgcg aatctccaga tctttttgcg caatcacaaa accgtcgtta ctgtcgcgca
    10321 gcacttgcag gcgaatttgc gccgttttag aaagcggcgt tttgtagagc agcacgcagt
    10381 gagaagccac cgcgccacga cctacgcgcc cgcgcagctg gtgtaactgc gccagaccca
    10441 gacgctcagg gttttcgata atcatcaggc tggcgttagg cacatcaaca ccgacttcaa
    10501 taacggtagt ggcaaccagc agatgtagct caccttgttt aaacgacgcc atcaccgcct
    10561 gtttctcggc tggtttcatc cgcccgtgta ccaggccaac attcaattct ggtagcgcca
    10621 gtttcaactc ttcccaggta gcttccgccg cctgcgcttc cagtaactct gactcttcaa
    10681 tcaacgtaca aacccagtac gcctgacgac cttcagttat gcaggcgtgg cgcacgcggt
    10741 caatgatgtc ggtacggcgg gtatcaggaa tagcgaccgt agtcactggc gtgcggcctg
    10801 gcggcagctc atctatcacc gaggtatcga gatcggcata cgccgtcatt gccagcgtgc
    10861 gggggatcgg cgtggcggtc atgatcaact gatgcggatg gaagccctgc tgctggcctt
    10921 tctcccacaa tgccagacgc tgatgcacgc caaaacgatg ctgttcgtcg ataatcacca
    10981 gcgccaggcc gttaaactgc acctgttcct ggaagatggc gtgtgtaccg acaatcatct
    11041 gcacctgacc gctggcgatg gcttcctgct gagacagtcg cgctttgcct ttctgcttac
    11101 cggcgagcca gcccacttcg ataccgagcg gtgcaaacca gttgcggaag ttattggcgt
    11161 gctgctcggc gagtaattcg gttggtgcca tcagtgccac ctgtttgccg tgggcaatcg
    11221 cacgcagcgc ggcgagggcg gcgaccagcg ttttaccgga acctacatcg ccctgtacca
    11281 ggcgcatcat cggcacatcc agcgccatat catgctcgat ctccgccact acgcgtgcct
    11341 gtgcgcccgt tggcttgaac ggtaaggcgg cgaggagttt atttttcagc gtgtcattgg
    11401 cgctcagcgg ctgggcatga aaacgctgtg ctccggcacg taaggctaac atgctgaggt
    11461 tgtgcgccag cagttcttcc agaatcagac gacgttgcgc cggatgctgc ccggtttcca
    11521 gatcgctaag ctgtcgcgtc ggtggcgggc ggtgcaaagt gcgcaacgct tccggtagcg
    11581 tcatcattcc ttgtgacagt tccggcggca ggagttcttc aatggcgcag gtgtcgagca
    11641 gatccagcgc ctggtcggtt aatttacgca gcgtggcctg ctttacgcct tccgttgttg
    11701 gataaaccgg cgtgagcgtt tcctgtaatt ctggcgtgct gagatcgccc tgcacgcggt
    11761 attccgggtg gatcatctcc gcaccatatt taccgcgctt tgcttcgcca taagccagta
    11821 cacggcggcc cgtcgccagg ctatttttca ttgccgcgct gaaattgaaa aagcgcatgg
    11881 tgaggatgcc ggaaccgtcg ctgatctggc aggtcatcat ccgccgaccg ccgaaggaga
    11941 tattgcagtt cagcacttcg ccttccaccg tggcataaac gcccggcagt agttctccga
    12001 tggggtagag atgggtgcga tcttcgtagc gcaggggaag gtgtaagagt agatcctgca
    12061 cggtatgcag gttgattttc gccagtttgt tactaagtgc tgcgccaacg cccgttaggg
    12121 aactgagtgg gacagcatct aacaggcgac ctttcatggc acttaccctg cagcctgcat
    12181 agtagcccac cagtcggcat cagcttcgat ctcgccttgc tgattgacgt ggggataagg
    12241 caggcctttg cgttttgcga ctttcgccag caccggatag ccgccttcaa acaacaggcg
    12301 ttgttgcgct gcttccggca acatgctgtt ttcacgcagg tacatgcctg cattttgccg
    12361 ctgacgctgg gcttcgtaaa gaatgagggc tgaggcaacg gaaacattca gcgactgcac
    12421 catgccgatc atcggaatga tgatgtcctg atccgccagg gccaatgctt cctgcgtgat
    12481 gcccgttttc tcctgtccca tcaaaatgca ggtcgggcga gtgtaatcaa tttcgcggaa
    12541 atcgacagca ttatcagaaa gatgggttgc cagaatctgc atgccctggc ctttgagatg
    12601 agcgacggca tcgccaatgg tgcggtgtgt tttcacctgt acccagctgt tactacccgc
    12661 cgctgccgaa gccatggtgc gcatgcggct accaggccag acggcgtgaa cttcatgtac
    12721 gccaacggca tctgcggtac gaataatcgc agaaacgtta tgaggtttgt ggacctgctc
    12781 catgcagacg gtcagatcag gctgccgcct ggcgagcatt tcgcagatgc gtgcataacg
    12841 tgttgggttc ataaaacatt aatttcggtt tcgggtgact ttaatcacgt ctggcatcac
    12901 gcggattttg cgcatgatat tcgccagatg cacacggtca cgagcggtca gacgaataaa
    12961 ggcgctgtag acgcgaccat ctttctcttc cgtattcaaa ctttgaatat tcgaagtcgt
    13021 ggtgttaatt gccgccgtca ggtttgccag cgcgccctga tgattgaaca tctccacctt
    13081 gatttcggtg atgaactcct gcgccgtctc tttatcccat tccacagcca taaacttctc
    13141 tggctctttc tggtagccac ggatattacg gcaggattca tggtggatca ccagaccttt
    13201 accggggctg acgtgggcga taatcgggtc gccaggaata gggcggcagc atttcgcaaa
    13261 ggtgatcagc acgccatcgg cacctttaat gggcagatgt ccgtggcttt gggttgccgg
    13321 tggaatggag gcgtccccat gttgcagatt tttcgcgacc accacgctca ttgcgttacc
    13381 aagtccgatt tctgccagca gatcgtcaag cgttgccagc ttcatgcgat ccagctcgcg
    13441 ctgaatattt tcctgcggga tttcattcag cttacggcta ccacccaaag catggttgag
    13501 cagacgacgg cccaggctta cagaatcatc acgcttgagg tttttcagca actgacgaat
    13561 tttggcgcgc gcttttgagc taacgacaaa gttcagccac gcggcattcg ggcgagcgcc
    13621 cggtgcggta atgatttcaa cggtttgacc gctggtaagc ggctgcgaca gcgggtaagg
    13681 ctggcggtca acgcgtgcgc ccacgcaggc atgaccgata tcggtatgca ctgcataagc
    13741 gaagtcgacg ggcgttgcac cggcaggcag ctcgacaatt cgcccttccg gtgtgaaaac
    13801 gtaaatctca tccgggaaga gatcggattt aacgctctcg ataaattcaa acgaactacc
    13861 ggcgctctgt tgcagctcca gcaggctttg catccagcgc tgggcgcgga tttgcgcggt
    13921 ggtgctggtt tcgccgtgct ctttataagc ccagtgcgcg gcaacaccca tctccgccat
    13981 ctgatccata tcttcggtac ggatctggac ctcaaccggc acaccgtgcg ggccgatcat
    14041 cgaggtgtgc aaagactgat agccgttcgc ttttggaatg gcgatgtagt ctttcacgcg
    14101 acccggacgc ggcttgtaca ggctatgcat ttgcccgagc acgcgataac aggtgtcgga
    14161 atcattgacg atcacgcgga aagcgtagat gtccatgatt gagtgaaaac gctgctcttt
    14221 gagcaccatt ttgcagtaaa tcgaataaag atgcttctcg cgaccactga cgcggcaggg
    14281 aattcccgct tcctgcaaac gcccttcgat ttccgagagg attttttgga tcatctcttt
    14341 acggttgccg cgcgcggctt tcaccacttc tttgattacg cgataacggt tgggatacag
    14401 cgcctcaaaa cccagctctt cgagttcggt tttaatgtgg tggataccta aacggtgcgc
    14461 cagcgggctg taaatttcga gagtttcacg ggcgatgcgg cgacgtttgt ccgggcgaag
    14521 tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt ttgatgagga tgacgcggat
    14581 atcctgcacc atcgccataa tcatcttgcg aaagttttcg gcctgcgcct ctttcttatc
    14641 gcggaacttg agtttatcaa gtttcgacac cccctctacc agctcggcga cgcttttacc
    14701 aaaaagctgt tccatatcct ggtaggtggc gggagtatct tcaatcacgt catgcagcag
    14761 cgccgccatc agcgtttcat agtcgagttt catctcggcc agaatgcagg caaccgctac
    14821 cgggtgcgtg atatagggtt caccgcttga acgtgtttgc ccctcgtgag catcacgtgc
    14881 aacgagatac gcctgccgca gacgcttgat ttggtcttcc ggcaggtagg tttgaatcag
    14941 ttgattcagg ctttcaaaca gatacaaggg cgacccgctt tgtgattaac gacgaccttc
    15001 agcaatagcg gtaacggctt gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc
    15061 gcgaacgtcg aggatctggt tgttgatcag accttcttcg atttcgcgca gcgcgattac
    15121 agtggtttta tcgttttctt ccggtaccag cggatccttt ccgcctacct gcatctgacg
    15181 agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta ccaattttct ctacagcgtc
    15241 ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg tgaagaaatg actgggcatg
    15301 atactgaaat caggttcagt ctgccaacaa tttgctgatt aaagcgtcat gacgctgctt
    15361 ttggcggctc atgcgcagac gttcggcgcg aataatggtc ttcaaatcgg tcaacgcggt
    15421 atcgaagtca tcattcacaa tcagataatc atattcggcg taatggctca tttctgcaac
    15481 agcttgcgcc atacgctttg caatgacctc ttcgctgtcc tgaccgcgac cgcgtagacg
    15541 gcggtccagt tcaattttgg acggcggtaa aataaagata ctccgcgcgt gcggcatctt
    15601 ctggcgaatt tgctgcgcgc cctgccagtc gatatcgaga aaaacatcga caccggtcgc
    15661 cagtacttgc tcaatggcct cacgcgaagt gccatagtaa ttaccaaaaa cttctgcgtg
    15721 ttcgaggaac gcatctctgc taatcatttc tttaaattca tcatgattaa caaagaaata
    15781 atgttcaccg tggacttcac caggacgcgg ttggcgtgtg gtgtgtgaaa cagaaacctg
    15841 ggtgtcatac aacggttggg tttttaataa agcctgaatc aggctggatt tacccgcgcc
    15901 actgggggca gaaacaatat aaagcgtgcc ttgagccatg agtatctttc gtatgtgatt
    15961 agcgaaataa agcctacata cgagcttatt atacacggcg cggccatgtg acgtagccct
    16021 tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag ctgtttccgc atttgcctgt
    16081 gatttactgc aataaagacg attctcagga agcgtcctcg cagactccgt ttttgccact
    16141 cggctttgcc ttcaccacgt tgtatatctg ccagcctgac tacagcggag gtgaagatga
    16201 aagtatggat ggcgatatta ataagtatct tgtgctggca atcatctgtg tgggcggtct
    16261 gtccggcctg gtcgccagcc agagcacagg aagaaatttc ccgcctgcaa cagcaaataa
    16321 aacagtggga cgatgactac tggaaggaag gaaaaagtga ggtggaagac ggtgtttacg
    16381 atcagttaag cgcccgtctt acgcagtggc aacgctgctt tgtgagcgag ccccgcgacg
    16441 tgatgatgcc gcctttaaat ggtgcggtta tgcatccggt tgcccatacg ggtgtacgca
    16501 aaatggcgga taaaaacgca ttaagtctgt ggatgcgaga acgtagcgat ctttgggtgc
    16561 agccaaaagt tgatggcgtt gcggtaacct tggtttatcg ggacgggaaa ctgaacaaag
    16621 caatcagtcg cggtaatggc ctgaaaggcg aggactggac gcagaaagtt agcttaattt
    16681 ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag tacgcttcag ggggaaatat
    16741 ttctccagcg cgaggggcat atccaacaac aaatgggggg aataaatgcc cgcgcaaaag
    16801 ttgctggcct gatgatgcgc caggggaata gcgacacgct aaactcactg gctgtttttg
    16861 tctgggcatg gccggatgga ccgcagttaa tgaccgatcg tttaaaggag ctggctaccg
    16921 caggttttac tctaacgcag aggtataccc gtgcggttaa aaatgctgat gaggttgcgc
    16981 gcgtacgcaa tgagtggtgg aaagcgaaat tacccttcgt caccgatggc gtggttgtgc
    17041 gaggggctaa ggaacccgaa tcccgtcatt ggctaccggg ccaggcggag tggctggtgg
    17101 cctggaaata tcaacctgta gctcaggttg ccgaagtgaa ggcgattcaa tttgcggtgg
    17161 gtaagagcgg taaaatatcg gtggttgcgt cactcgcacc tgtcatgctg gatgataaaa
    17221 aagtccagcg ggtgaatatt ggttccgtca ggcgctggca ggagtgggat attgcgcctg
    17281 gtgatcagat cctcgtcagc cttgccggtc agggtattcc tcgcattgat gatgtggtct
    17341 ggcgcggtgc agaacgtaca aaaccgacac caccagaaaa ccgctttaac tcgttgacct
    17401 gctactttgc ttctgatgtt tgtcaggaac agttcatttc acgcttagtc tggctggggt
    17461 caaaacaggt tctggggctg gatggcattg gtgaggccgg ttggcgcgcg ctgcatcaga
    17521 ctcatcgctt tgagcatatc ttttcctggc ttttattaac gccagagcaa ttacagaaca
    17581 cgccggggat cgcgaaaagt aaaagtgcgc agttatggca tcagtttaat ctggctcgta
    17641 accagccttt tactcgctgg gtgatggcaa tgggaatacc gctaacccgg gcggcgctta
    17701 atgccagtga tgaacggtcc tggtcgcaac ttttatttag cacggagcag ttctggcagc
    17761 agctgccggg gactggatct gggcgcgcca gacaggttat tgaatggaag gaaaatgcgc
    17821 aaatcaagaa gctgggcagt tggctggctg cccagcagat cacaggtttt gaaccttagt
    17881 gaccttcgtg gctgtagtaa aagaccggta atcccagctt aagacgtagc gccagtaaac
    17941 gagcaaaaaa gccgaatact agggtggaga taatcaccac atcatggtta gaaacatagt
    18001 gttgcagcgc gatgtacagc acggcggagg caaatgagac accggcatac aactcttttt
    18061 gaaagaccag cggaatgcgt ttacagaaca tatcgcgcaa aacgccgcca aatacaccgg
    18121 tggttaccgc cgcgacaacg gcaataattg ggccgtgccc catatccagt gcaacctgtg
    18181 cgccgatgat agaaaagacc accagtccga gcgcatcgag caccagaaac actttgcgca
    18241 ggtaaggcat tacaggagca acgatagtgg tgagtactgc ggcggtagcg acgataatca
    18301 catattccgg gtgtttgacc cagccgagcg gatagtggcc cagcagaata tcgcgcactg
    18361 accctccgcc aattgcggtg gcggttgcaa taataattac gccaaatgta tccatgcgcc
    18421 gtcgcccggc cgccagcgcc cccgtcatgg cttcggcagt aatgccaacc aaatacaaaa
    18481 tgtgtaacag catggttctc tccctaaaaa tctgtctggc agggtagcga tttgtgcgca
    18541 ttgtcacgat tgatattttt taaggtgaga ggaaattgat aacttttagt tatgtgaaat
    18601 cgctattttc tgtagcgtag atgcattctt ctgacttctt gtctgagaaa atgcataaga
    18661 aaaaataggt taaaactgag gggggaaaag aaaggtgcaa tgtttagcca ttgtgttcac
    18721 tgcacctgga ccttggctta cgcatcgggt gtctcaatca acaaaaagag tttttcgttt
//
LOCUS       DADKXM010000073        18208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.074, whole genome shotgun sequence.
ACCESSION   DADKXM010000073 DADKXM010000000
VERSION     DADKXM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.074"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1019
                     /locus_tag="JQR05_002464"
     CDS             <1..1019
                     /locus_tag="JQR05_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY5324616.1"
                     /translation="FSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQC
                     WEPDVEWVRQPPQDAITAPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADE
                     SYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQNLCNP
                     LYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAG
                     HDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQ
                     TQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGIL
                     CNND"
     gene            992..1621
                     /locus_tag="JQR05_002465"
     CDS             992..1621
                     /locus_tag="JQR05_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAY5324617.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEDTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1622..2782)
                     /gene="ybdL"
                     /locus_tag="JQR05_002466"
     CDS             complement(1622..2782)
                     /gene="ybdL"
                     /locus_tag="JQR05_002466"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY5324618.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            2891..3979
                     /gene="hcxA"
                     /locus_tag="JQR05_002467"
     CDS             2891..3979
                     /gene="hcxA"
                     /locus_tag="JQR05_002467"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY5324619.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3989..4186)
                     /locus_tag="JQR05_002468"
     CDS             complement(3989..4186)
                     /locus_tag="JQR05_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY5324620.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4368..6462)
                     /gene="cstA"
                     /locus_tag="JQR05_002469"
                     /pseudo
     CDS             complement(4368..6462)
                     /gene="cstA"
                     /locus_tag="JQR05_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
     gene            complement(6643..7056)
                     /gene="entH"
                     /locus_tag="JQR05_002470"
     CDS             complement(6643..7056)
                     /gene="entH"
                     /locus_tag="JQR05_002470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY5324621.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7059..7805)
                     /gene="dhbA"
                     /locus_tag="JQR05_002471"
     CDS             complement(7059..7805)
                     /gene="dhbA"
                     /locus_tag="JQR05_002471"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY5324622.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(7805..8662)
                     /gene="entB"
                     /locus_tag="JQR05_002472"
     CDS             complement(7805..8662)
                     /gene="entB"
                     /locus_tag="JQR05_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY5324623.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8676..10286)
                     /gene="entE"
                     /locus_tag="JQR05_002473"
     CDS             complement(8676..10286)
                     /gene="entE"
                     /locus_tag="JQR05_002473"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY5324624.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGHLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10296..11471)
                     /gene="entC"
                     /locus_tag="JQR05_002474"
                     /pseudo
     CDS             complement(10296..11471)
                     /gene="entC"
                     /locus_tag="JQR05_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            11762..12718
                     /gene="fepB"
                     /locus_tag="JQR05_002475"
     CDS             11762..12718
                     /gene="fepB"
                     /locus_tag="JQR05_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY5324625.1"
                     /translation="MRLAPLHRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGAATPNNRVADDQGFLRQWSKVAKERKLQQLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12722..13972)
                     /gene="entS"
                     /locus_tag="JQR05_002476"
     CDS             complement(12722..13972)
                     /gene="entS"
                     /locus_tag="JQR05_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY5324626.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14083..15087
                     /gene="fepD"
                     /locus_tag="JQR05_002477"
     CDS             14083..15087
                     /gene="fepD"
                     /locus_tag="JQR05_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY5324627.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15084..16076
                     /gene="fepG"
                     /locus_tag="JQR05_002478"
     CDS             15084..16076
                     /gene="fepG"
                     /locus_tag="JQR05_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY5324628.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPHSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16073..16888
                     /gene="fepC"
                     /locus_tag="JQR05_002479"
     CDS             16073..16888
                     /gene="fepC"
                     /locus_tag="JQR05_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY5324629.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16885..18018)
                     /gene="fepE"
                     /locus_tag="JQR05_002480"
     CDS             complement(16885..18018)
                     /gene="fepE"
                     /locus_tag="JQR05_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY5324630.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4301 a         5152 c         4710 g         4045 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
     1141 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
     1201 acccgaaaaa aaactgctgc aaaaatccat tgaaatagat ggttttaccc aacccattgt
     1261 ggtcacacat acggataaaa atgccatgga aatcgtggat ggttttcacc gccatgaaat
     1321 tggtaaaggc tcatcatcat taaaattacg cttaaaaggt tatttgccgg tgacctgtct
     1381 ggaagatacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg ctcgtggacg
     1441 ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg gatgggacga
     1501 taataaaatt ggcaaagagc tgggaatgga cagcgacgaa gtgctgcgcc tgaagcaaat
     1561 taacggcctg caagagttgt ttgccgaccg tcaatattcc cgcgcctgga cggttaaata
     1621 gctaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc ttggcaaaac
     1681 agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc ggaatcgccg
     1741 ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg gtagaaaccg
     1801 cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc agccggcttt
     1861 catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt aacgcaagat
     1921 aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg gtattcaccg
     1981 aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct ggcgcaacac
     2041 aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa accgccactg
     2101 cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt gaaaagttga
     2161 tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc gcctgccaca
     2221 aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcgga gtgttgagga
     2281 tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca acgcgaaaat
     2341 gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt
     2401 aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa
     2461 tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg
     2521 gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc
     2581 cggtcatggg cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta
     2641 aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct
     2701 gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg
     2761 gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact
     2821 ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg
     2881 acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc
     2941 acatccagga agtttcaatc acctgcacaa ttttttcact gatgaacaac tttctcgcgc
     3001 ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt
     3061 tggacttcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca
     3121 gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc
     3181 actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccatttg ttgccgctcc
     3241 gacgatcgcc gccacctgcg cagcctggac accgctctcc gtctggtata acgatgccgg
     3301 acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga
     3361 gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa
     3421 atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcggct
     3481 ggggatcaat aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg aacaggcact
     3541 tgccgatcag caaaatcagc agttaacgca atcattttgc gatgtggtgg atgctattat
     3601 tgctggtggt gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca
     3661 tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag aagtttcttc acggcaccaa
     3721 agtcgcttac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca
     3781 attaaccggg gcgtatcagc gttttcatct gccgactacg ctggcggagc tggaggtgga
     3841 tatcaataat caggcggaga tcgacaaagt gattgcccac accttgcgcc cggtggagtc
     3901 cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcgaaaaagt
     3961 ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct tttccaccgt
     4021 agcgcgcgtc ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat
     4081 gattaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg
     4141 ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta
     4201 accatgttgc tgaaagagtg ccggatgcgg cgtgaacgct ttatccggcc tacaaatgct
     4261 atcaacgcac tcggtcagtc ccctcgcccc atcggggaga gggttagggt gagggggaac
     4321 cctcggcact atgccaacat tcgccaacat cccccctcac tctgacttta gtgcgcgcct
     4381 ttcgcctgcg ctacgatctc ctcgacattt tccggcatcg gctcatacgg cgtttctttc
     4441 gccgttggct tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga
     4501 accaccacga ccaccataaa gaagatggtt aacccggcat ccagacggtt gttgaacacc
     4561 agttgcgcca gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc
     4621 tggaacttgt tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc
     4681 cagtcagcgg tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg
     4741 taacgttgac gtttcatctt gaacaacacc acggcacaga gcatcagcgc catccctgcc
     4801 agcatctggt tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg
     4861 accacgccct gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg
     4921 ttagcaggca gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc
     4981 aacataaagc gcgcagcacg cgtacccgca tctaccgccg tcagaataaa cagagcttca
     5041 aacagaatgg cgaagtgata ccagaacgcc acatccatca taccacccag cgcaccgtgc
     5101 aggatgtaag ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga aatgatcgac
     5161 tgttcaccca cttcgctggc aatctggttg agcgtatctg gtgtaatgct aaagccccag
     5221 ctactcacca tctgcgcggc agaagcgact acatccgccg tccctgccgg agccagcacc
     5281 gccattgggc tgttcatggc aaaatacacg cccggatcga tgatacaggc agaaaccagc
     5341 gccataatcg ccacgaagga ttccatcaac atcccaccgt agccgataaa gcacgcctgc
     5401 ccttcgttcg ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg gaagccagac
     5461 accgcaccac aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg
     5521 ccagtgccat cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa
     5581 atgcctaccg ccagaccgac gatagtcccg attttcagga aggtcgagag atagtcacgc
     5641 ggggccagca gcaaccacac cggcagcacc gccgccacaa aaccgtagcc caccagcatc
     5701 caggtcagct gcacgccggt aaagtcaaag tacggtgccc agtgccaccc agccgccaga
     5761 gataatggcg aaaatcagga acaccaggcc aatcactgac acttcgccaa tgcggcccgg
     5821 acgcagatag cgcaggtaga ttcccataaa aatcgccagc ggaatggtga acgcaacggt
     5881 atacgttccc cacgggctat gagtcagggc tttcaccacg atcatcgcca gtactgcaag
     5941 gataatgacc atgatcataa agcaggctac cagcgcaatc accccggcag ttggccccat
     6001 ctcttctttg accagctcac ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac
     6061 catgaaatcc tgcaccgcac cggcgagaac caccccggcg agcagccaga tcatccccgg
     6121 caggtagccc atttgcgctg ccagcaccgg ccccaccagc ggccctgctc cggcaatggc
     6181 cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc acatagtcca gcccgtcgtt
     6241 atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata
     6301 cagcccataa aaacggtaag cgatcagata gatacagacc gacgccacca caatccacag
     6361 cgcgttgatc tgttccccac gatttaaagc aatgtatccc agagcaaatg ctcccattac
     6421 agagagcact gtccagacga ggtatttccc tgatttgttc atagttgtta tccgtgtgcg
     6481 tgtccataga gatgttacat tttgtttcta taacatcttt gtacgattta acaacacggt
     6541 aaccatgtaa atcggagttg aaaccaatat ttaacttaac aatgttaact cgatcactcc
     6601 gttaaccgag ttttcctgca atctcattta attctgtcgg gctcatccca aaactgccgt
     6661 acccagccga caagtgcagc aacgccgccc ctgttcatcg aaaacgacga tttcccagct
     6721 ctgattttgc cgcccaagat gcagcggctg gcagacgccg cgtaccttgc cttcagacac
     6781 cgggcgatga tgggtggcgt taagttccgt gcccaccaca cactgcccgt cacgggtcat
     6841 cataaatccg gccatcgatc ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag
     6901 caggccaaac ggctgatgag tacgggtatc aaccggcatt tcggcttcca gcacatcatc
     6961 tcccagacgg gtatacacaa ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt
     7021 cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt atgcccccag cgttgagccg
     7081 ccatcgacca caatatcctg tagggtgata tggctggcga ggtcagaggc gaggaacaaa
     7141 atcgtgttgg cgatctcttg tggacgggcg attttcccca gcggaatgcc gagtttaaac
     7201 tgctcgccaa agccgcgaat acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt
     7261 tgcatatcgg tgtcggtgga gccaggcgaa accacattac agcgcacgcc gctacccgcc
     7321 agttccagcc cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc gccataagca
     7381 ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc
     7441 cgctgacggc gaaactggtt catggtttgc tggaacaggt taaacgcacc gccgacgtta
     7501 accgcaaaag tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa
     7561 attcccgccg cattgatcag cacgtccagt cgctccgttt cagctaacag tcgctgacat
     7621 acctgcgcca cctgcccggc gtcggcaaca tccattactt cggtcgcaaa gggatattgc
     7681 tcctgagtga acgcttgatc aaaacctgta actttcgctc ccgcctcaac aaacgccagc
     7741 gccgtggcgt agccgatacc tttacctgcg ccggttaccc agacattttt accgctgaaa
     7801 tccattattt cacctcgcgg gagagtagct tccaccaggc gtcgatggtc gggtttttcg
     7861 ccagcatgac aaagtcgata tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca
     7921 tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc atcgaacggt tcatcggact
     7981 cgtccagcaa cggcagaatc acctcacgca gcgccgcttt gctggcgggg ataggtgctg
     8041 gcagtaattc ttcggtcatc accacccggc cagaacgtcc ggccacatat ttcagcgaca
     8101 tcaaatgctc gtcacggctg aaatcagcca gcgcatccgc caccataaac ggtttaatat
     8161 cgcgcataaa tgcgtcggtt gcggtggtca tacagccaat gtgggcatat acccctgtaa
     8221 taatcagctg gttacgtcca ctctctttga gcatttgctc cagcggagaa cgatgaaacg
     8281 cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca
     8341 ccttttgctg ttccggcgag cgggtcaggc ccggccccca catatcattc aacagcgccc
     8401 gatcttcatc gctctgctct ttcggctggg cggtgtaata aaccgggata ttgtgctgtt
     8461 tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg ctccatcatc gggcagttct
     8521 cgccccagaa gctgacaaaa tagtcctgca tatcatggat taacaacgcg gcacgttgcg
     8581 gttcaaaggc ccagtcaact ttattctgcg gaatatcgtg agactccggc agtgcgtaag
     8641 cctgtaattt tggaatagcc atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc
     8701 cactgacgta attgtttttt atcgactttc ccgaccgccg tcagcggaag tgaatccaca
     8761 cactccacgc gatccggtaa tttaaattcg gcaataccct gttcacgcag gaaacgacgc
     8821 acctgcaccg cgcgcagcgg ctcttttacc accagataag cacagctttt ttcgcccatc
     8881 agctcatctt ccatgctcac cagtgcggca tagatcactg ccggatggcg cagcagcagg
     8941 ttttcgatat cttcggcagc gatcttctcg ccgccacggt tgatctgatc tttctcgcgc
     9001 ccctgcacgg tgatgtaacc ctcaggatca atagagatca gatcgccgga acagtaaaaa
     9061 ccgttggcat caaaggcact ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg
     9121 tacggcccgc gcgtcatcag gtgtccaact tccccttgcg gcagtggatt tccttcggca
     9181 tcggcaaccc acacttcatc gtccggacac attgggtaac cctgggtatg gataattttc
     9241 tccgcgctat catcaagacg ggtgtagttc accagccctt ccgccatgcc aaacacctgc
     9301 tgcaactggc agccaatctc agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg
     9361 ccgccgacct gtaacagttt cagcgaggca agctgcgccc ggctttcgcc ttcagccagc
     9421 gcctgcaacc acaggctgac tgccggcggc accagcgcgg taacgttaac ctgatgtttt
     9481 gcaatcaacg ggaagcaaag cgtggcgctg ggatcggccg ccagaacaac cgttcctccg
     9541 gcaagaaaga cgcccagcga tcctggcgaa ctcatagcgt agttatgagc cgccgggatc
     9601 gcgcacaggt agcgcgtctg ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg
     9661 tagtaatagt cgttatgagt gcgcgggatc agtttcggtg tgccggtggt gccgccggaa
     9721 agctggaaat aggccacttc atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga
     9781 tggttaatcg catcctgcaa gttatgctca ccgctgtcgt tgtgcagttg caccacgcga
     9841 atggaagaat gctctgcaac aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc
     9901 tggcgatcgg caatcagtaa cgccggttca atctggctgg cgtaggcgtt cagttcacta
     9961 cgctgatggc tgaacagcgc cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag
    10021 gtaatgtaaa gttcagcgac gttacccagt tgtaccagcg cggtttcacc aggtttaatg
    10081 ccctgacggc gcaaactaca ggcgaggtta tccgccgcct gattcagctc ccgataactc
    10141 aactgccgct cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg
    10201 tcggtcagtg gcaaatcctg ccagtagcct ttttcccgat agcgacgggc aaactcttcc
    10261 ggccagcggg tgaatggaat gctcatcctc gctccttaat gcaatccaaa aacgttcaac
    10321 atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca acggtgacga cgcaggcaca
    10381 atccccgctc cggcaaacag acgcacctga ttttcccgct gcttcgcgca gcggatggtc
    10441 accacccatt cgccgttacc ttcgctgtca caccaaccca caatgccgcc aaacagttcg
    10501 cggtcgaacg gctccagttc agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc
    10561 agcgcggggg tcggatgcag cagacaggcc agagtcagtg cgttttcttg cgaattcgct
    10621 ttaccttcaa agggagttgc gagatgccac agcgtcggcg tggtaatcaa ttgtggagag
    10681 gagggaacgt gtaactcact actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc
    10741 agttcatgtt catggcgatc tttttctgac gccagcagac gattacccgc ttcgcgatcg
    10801 agcacttcat ccggctgacg acgcgcggaa ccggctaacg gaatggagct aaaacgctcg
    10861 ccgtctttac gtagcagcag ttccgggctg gcccccagca ggacgccacc atcagccagc
    10921 gggacatgga agttgtaact aaccgggttt tgcgcaatca accgttccag caatacgcca
    10981 ctatcaatgg cggcgtcagt ggtgatatca atcaatcgtg acaacaccac tttgtcgacc
    11041 tgcggcgtgg cggtaagtgc ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca
    11101 ggaattgcct ggcgttccac cacgttcagc gactggctgc gggtaaaaca gcgggctgag
    11161 gtctgttttt cctgacggga gaacgactgc taggattcgg gaatatacag cgacgaaggc
    11221 tgacgtggat cgaagggaat cgccccgacc atcaccggat ttttgatgcc ctgcgctttg
    11281 gcatcggcaa acagcgcggc gagtttttgc tggaagggac tgtcgggcga atccccgttc
    11341 acagccggtt catcgaaacg ggcgaaacat cctgacgtcg taaaactgcg gtacggcgat
    11401 ataaagaaaa agcgattggg cgcaagtgtt gccatggtct gctgtacttc ctcagccagt
    11461 gacgtatcca tatcatcctc cacaaatgat aaaggcttta ataatgatta tcatttatat
    11521 tttcggccgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct
    11581 gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt attgatgaat
    11641 tcgcataagc gcaatgtgat gtcctgcgcc gttttgcccc ctctccccaa agggacggat
    11701 tgtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact
    11761 tgtgagactc gccccgctcc accgcaacgc ccttctatta acaggacttt tgctttcagg
    11821 aatagccgta gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca
    11881 taccctggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact
    11941 gctggcgatt gatgctccgg tgatcgccag cggcgcggcc acgccgaata accgcgtcgc
    12001 agatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaaca
    12061 gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt
    12121 aattagcgca accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc
    12181 cccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg
    12241 cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact
    12301 ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac
    12361 cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga
    12421 acaactcggc tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg
    12481 caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgcggggt taaacggtga
    12541 atcgctattc ctgttcgctg gtgatcagaa agatgccgat gcgatttatg ccaatccact
    12601 gctcgcgcac ctgcctgcgg tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt
    12661 ccgcctggat tactacagcg ccatgcaagt gctggaacgg cttaaggcgc tgttttaagc
    12721 attaactgtc ggacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca
    12781 gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg cttgcggaag
    12841 caaccggtgt catcatcgcg cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg
    12901 tcacgttctg cgccgtccac aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg
    12961 tttgcagcat tgtatattgc agcaacgaac taaccgcgct cagccagccg aacagcgcca
    13021 gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg gcgaggaacg
    13081 atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc agcttcccgc
    13141 tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg
    13201 ccgctgacat ctgccagttg tcagccagcg ccggatacag taccagcacc gcgctcgcca
    13261 tcgtcaataa accacccagc agcgcaatcc cgcccaccag cgggctggca agcagaaaac
    13321 gaaatcctgc cagtaatgat ttcaacggat gctcacgcgg ctgcggtggc ggtggcaacg
    13381 ccggaaggct taataacggt aacaaggtaa taaacgtgcc cgccgccgcc agcccgtagt
    13441 tccaggctac gccgccggtc gccagcaata aaccgccaat catgggcgaa atcaccgacc
    13501 ccaggcgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca cgccctacca
    13561 gtgcagatgt tgccgccagt agcgccgtaa caccaagtga tgcgaaaaaa ccatcccata
    13621 aaccaagtaa atagattgcc agcaatgacg gctccggcag cagtgcatta aggcacagtc
    13681 caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgttcatagc
    13741 gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg gtcagcgtca
    13801 ccgaaagccc cacctgccag gtagaatggg tcatcatctg gatctgcacc ggcaccgcga
    13861 cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact gcgcgaaacg
    13921 ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc
    13981 cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata
    14041 agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc
    14101 gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta
    14161 agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc cttctccggc
    14221 acctgccaga gcgccgactg caccatcgtg ctcgacgcgc ggctgccgcg tacccttgcc
    14281 ggtttactgg caggcggcgc gcttggcctt gccggggcgt taatgcaaac cctcacccga
    14341 aacccacttg ccgaccccgg cttgcttggc gtgaacgccg gagccagctt tgccattgtg
    14401 ctgggtgcgg cgctgtttgg ttactcatcc gcacaggaac aactggcgat ggccttcgcc
    14461 ggggcgctgg tggcctcatt gattgttgcc tttaccggca gtcagggtgg agggcagtta
    14521 agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga aggactgacc
    14581 agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt
    14641 tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt tctgatcgcc
    14701 ggagcaactg cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg cagtgacacc
    14761 gcgacggcgc tgggcagtcg cgtggcgcgc acacagttga ttggtctgct ggcgattacc
    14821 gtgctttgtg gtagtgcgac ggcggtagtt ggcccgattg cctttatcgg cctgatgatg
    14881 ccgcacatgg cgcgctggct ggtaggtgcc gatcatcgct ggtcgctgcc cgtcacgcta
    14941 cttgctaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc
    15001 gaactgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat cttcctcgtg
    15061 cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt
    15121 tgctgctgat ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca
    15181 cgctggaaat ctcgcaggta ttcgccgcgt tgatgggcga tgcgccgcac agtatgacga
    15241 tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcggcgc
    15301 tgggcgtcag cggcgcgatt tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg
    15361 taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc
    15421 aggatctgac ggctatcgcg ctggcagcaa tggtgggcgg cattatcact tcgctgttgg
    15481 tctggctgct cgcctggcgt aacggtatcg acacctttcg gttgattatt atcggtatcg
    15541 gcgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg tctttagaaa
    15601 cggcgctaac agcaggtttg tggaatgccg gatcgctcaa cggcctgacg tgggcaaaaa
    15661 cctcgccttc cgcacccatc attatattga tgctcattgc cgccaccttg ctggtgcggc
    15721 gaatgcgctt gctggagatg ggcgatgata ccgcctgcgc gctgggcgtc agcgtcgagc
    15781 gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca acggcgcttg
    15841 ccgggccgat ttcctttatt gctttagtcg cgccgcacat tgcccgacgc attagcggca
    15901 ccgctcgctg ggggctgacc caggcggcgc tgtgcggagc gctgttactg ctggcggccg
    15961 atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca
    16021 gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa aaatgaccga
    16081 atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat ataccgttgc
    16141 ggaaaatctg actgtagaaa ttcctgatgg tcactttacg gcaattatcg gaccaaatgg
    16201 ctgtggtaaa tccacgttac tgcgtacctt aagccgcctg atgacgcctg ctcacgggca
    16261 tgtctggctg gatggcgagc acattcaaca ttacgccagt aaagaagttg cacgccggat
    16321 tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg agctggtggc
    16381 gcgtggacgt tatccgcatc aaccgctgtt tacccgctgg cgcaaagagg atgaagaagc
    16441 ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg gcagatcaaa gcgtggatac
    16501 cctttctggc ggacaacgcc agcgagcgtg gatcgcgatg gtgctggccc aggaaacggc
    16561 aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatctact
    16621 ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg tgctgcacga
    16681 tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag ggaaaattgt
    16741 tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct
    16801 gcgatgcatg atcattgacg atccggtagc cggaacgccg cttgtggtgc cgctcggacg
    16861 aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt
    16921 tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag
    16981 cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg
    17041 gcttaactga tatttaaacg gcgtaaaatt cacatcgttg acatttgctt ttgttaattg
    17101 ctcgacaaga tactgccgat tacgtaattc accgttcagt tccgcaacgt cagtaaccgc
    17161 tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg
    17221 gtcatcttta acggcctgac cattactgta tacgggcttt ttaattcctg ccgcgttggc
    17281 aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt tcgttttaat
    17341 gcgatcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt tattacggac
    17401 gttttctatc gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac
    17461 ggtctgcgcc tctttactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga
    17521 cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc
    17581 aacaatagcg cgatgcaaat ccagttcatc gattttcgcc tcttttaatt ggtccatcac
    17641 ataaggtgat gaacgcaggt actcttccag caagctaaca gactgaaact tcttgataaa
    17701 caggttaaat gcaccgccac ggtcgatatt aacatcgaga tcaagaacgc ggagtttggt
    17761 aaaggttttc tccaggtcct gccactgaat ggcttcggcg ggagtaataa cggctgagct
    17821 ggtccatttt tgcggcagga tgaaagagat cagtaaacct gcgcaggcaa atgcaaaaac
    17881 gaccgccatg accgtttttt tggcccgcca taaaacttcg attagattaa gtaaatcaat
    17941 ttcattatta ctgggcgacg ccagaggata ctcagaaaaa tgagcgtcac ttccctgttt
    18001 aatattcaat gatgacatgg ggtataacct gaaaatgagt ccaatagggg aaaatctcca
    18061 cggcgctcac tttaccagtt ggtgagaatt atccgaagct gaagtttgta aattccttcc
    18121 actgaatgca acattcactc tatttaagag aaatattcat caaacctata aatcaaacaa
    18181 ataaaaaacc cataattatt ttctgtaa
//
LOCUS       DADKXM010000074        18203 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.075, whole genome shotgun sequence.
ACCESSION   DADKXM010000074 DADKXM010000000
VERSION     DADKXM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.075"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            481..1137
                     /gene="pphA"
                     /locus_tag="JQR05_002481"
     CDS             481..1137
                     /gene="pphA"
                     /locus_tag="JQR05_002481"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY5324631.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1138..1413)
                     /locus_tag="JQR05_002482"
     CDS             complement(1138..1413)
                     /locus_tag="JQR05_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY5324632.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(1485..1670)
                     /locus_tag="JQR05_002483"
     CDS             complement(1485..1670)
                     /locus_tag="JQR05_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY5324633.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVL"
     gene            complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JQR05_002484"
     CDS             complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JQR05_002484"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY5324634.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JQR05_002485"
     CDS             complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JQR05_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY5324635.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JQR05_002486"
     CDS             complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JQR05_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY5324636.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAE
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7322..7819
                     /locus_tag="JQR05_002487"
     CDS             7322..7819
                     /locus_tag="JQR05_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY5324637.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFVRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7916..8614
                     /gene="proQ"
                     /locus_tag="JQR05_002488"
     CDS             7916..8614
                     /gene="proQ"
                     /locus_tag="JQR05_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY5324638.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8634..10682
                     /gene="prc"
                     /locus_tag="JQR05_002489"
     CDS             8634..10682
                     /gene="prc"
                     /locus_tag="JQR05_002489"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY5324639.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQTAPVK
                     "
     gene            10874..11755
                     /gene="htpX"
                     /locus_tag="JQR05_002490"
     CDS             10874..11755
                     /gene="htpX"
                     /locus_tag="JQR05_002490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY5324640.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11801..13173)
                     /locus_tag="JQR05_002491"
                     /pseudo
     CDS             complement(11801..13173)
                     /locus_tag="JQR05_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13350..14141
                     /gene="kdgR"
                     /locus_tag="JQR05_002492"
     CDS             13350..14141
                     /gene="kdgR"
                     /locus_tag="JQR05_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY5324641.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14284..14523)
                     /locus_tag="JQR05_002493"
     CDS             complement(14284..14523)
                     /locus_tag="JQR05_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324642.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14682..14825
                     /gene="mgrB"
                     /locus_tag="JQR05_002494"
     CDS             14682..14825
                     /gene="mgrB"
                     /locus_tag="JQR05_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY5324643.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14900..15187
                     /locus_tag="JQR05_002495"
     CDS             14900..15187
                     /locus_tag="JQR05_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324644.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15857..16000
                     /locus_tag="JQR05_002496"
     CDS             15857..16000
                     /locus_tag="JQR05_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY5324645.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16013..16222
                     /gene="cspE"
                     /locus_tag="JQR05_002497"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16013..16222
                     /gene="cspE"
                     /locus_tag="JQR05_002497"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY5324646.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16388..17197
                     /gene="rlmA"
                     /locus_tag="JQR05_002498"
     CDS             16388..17197
                     /gene="rlmA"
                     /locus_tag="JQR05_002498"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY5324647.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17194..17760)
                     /gene="mntP"
                     /locus_tag="JQR05_002499"
     CDS             complement(17194..17760)
                     /gene="mntP"
                     /locus_tag="JQR05_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY5324648.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4823 a         4348 c         4583 g         4449 t
ORIGIN      
        1 cccaaatcgt aaaggaaacc gtagcacgtc gtatgcaaga acgtgccacg gctggctgat
       61 ggatgttcga tagcgcgagt ttgaatgaaa atcagccgga gatgatttta cataattgct
      121 acggaattat tcaatacagg aattgcttgc gtctgcatgg attgacctga aatattcccg
      181 aaaatttctc taaaaaactc gaaaaaaatg gtaactggtt gaatgtatta atatgcaatg
      241 gtacgtgtca gggattaaaa gatgaacgta aatttattca acgcattaat tttaaagggt
      301 tttattgttt gttgacgaaa acaggaatcg tgttcggtct ctttttatct gttaaaagcc
      361 agaagcattt ccttcgctga ctttatagtc aaccataaca cacactctac tgtctgagtc
      421 cagcgttttt taacattctt gttaagatta tgtgatcttt agcgcgggag gaaaatattg
      481 atgaaacagc ctgcgcccgt ttatcagaga attgcgggtc atcaatggcg acatatctgg
      541 ctttctggcg atatacacgg ttgtcttgag cagttgcgcc gcaaattatg gcattgtcgt
      601 tttgatccgt ggcgagattt acttatctca gtgggagacg ttatcgatcg tgggccgcaa
      661 agtttacgtt gtttgcagtt actggaacaa cattgggttc gtgcggtaag aggcaatcat
      721 gaacagatgg cgatggatgc gctggcattc cagcagatgt ctttgtggtt gatgaatggc
      781 ggcgactggt ttattgcgct ggcagataat caacagaaac aagcgaaaac ggcgctggaa
      841 aaatgtcagc atttgccctt tattcttgaa gtacacagtc gcaccggcaa gcatgttatt
      901 gctcatgccg attatccaga tgatgtttat gaatggcaaa aggacgttga tttgcatcag
      961 gtcttgtgga gccgctcgcg attaggtgaa cgcccaaaag ggcagggaat tacaggtgct
     1021 gatcatttct ggtttggtca tacaccgttg cgacatcgcg tggatattgg caacctgcat
     1081 tatattgata ccggtgctgt ctttgggggc gaattgactc ttgtgcaact gcaataatta
     1141 aaaatcacca tactcctgtg ctggtcgcca gaaaccatct ataaaatcct caatcggaaa
     1201 acaaccgcca tggcggatcc gttgatcgct catagaataa agacactgct gttccgtgtt
     1261 gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa aaagtaccag
     1321 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
     1381 acgggggaat aaccaggaca aataacccgc catgccgacg ggttattttt ggatcaggca
     1441 agacgcataa cccaggcatc agattgttga tcgtaacggg tacgctaaag gacagagtgt
     1501 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
     1561 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
     1621 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
     1681 tccatttagc tgccgattgt ggtacgacca gtccgatcgc acaatttttc agcctagttg
     1741 aggccgccga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
     1801 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
     1861 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
     1921 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc ctgcggtgtc
     1981 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
     2041 cgataacctt tgttatgcgt ttcggcaagt ttaatcccca agcgagaaaa tcggacttta
     2101 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
     2161 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
     2221 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     2281 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     2341 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     2401 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     2461 gcttcgtttt cttcccggtt taaggtacag gtcgagtaaa ccagcgtacc gccaggacgt
     2521 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     2581 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     2641 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     2701 tcaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     2761 tttacccgac tggcggaaaa ctcattggca aggattgccc cttcgttatt catccgcgcg
     2821 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ttgtggtgca
     2881 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     2941 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     3001 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     3061 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     3121 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     3181 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     3241 tggcaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac gccggagcag
     3301 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     3361 caacaggaag tgtttgcctt cctgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     3421 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     3481 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     3541 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     3601 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     3661 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     3721 agtaatggtg gcctcttgta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     3781 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     3841 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     3901 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     3961 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     4021 gtgaagcgta atctgcccgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     4081 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     4141 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     4201 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     4261 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     4321 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     4381 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     4441 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     4501 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     4561 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     4621 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     4681 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     4741 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     4801 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     4861 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     4921 gcgggtattt tcacgaagca gggtaacgac gctgggatca acggtcattt ccccagtgac
     4981 tttaccacca ggatttaaat ccagtttagt cagctgtccg acttccagcc cctgatacat
     5041 taacggcgtc gagtcggcgg ttaatcctgc tccactcggc agttccagtt ttattattac
     5101 tccacgctgg ctgtgggcca gatcttcata cagaccaaag gtatcttccg cctcggcagg
     5161 tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg ccagactttc
     5221 cagtttcacc ttcgcgccac tgatactgac gttggcatca acgccggaaa cgttccagaa
     5281 acggctacct tttttcacca gatcggtaaa acgccgctcg atcaggacat caatcaccac
     5341 gccttgcttg ttgggattga tggcatagtc gtagactttt cccaccggga tcttgcggaa
     5401 atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag
     5461 atcgccattg tccagccgat atttcggttg ggtatcgagc gcgacaaagt gatcctgctc
     5521 tttaccttta cccggcatca tgccgatata gttcccaccg acgagggcgt ccaacccgga
     5581 gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt cgcgcagcgc
     5641 atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat cgtcgctgag
     5701 gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg tacggcccgg
     5761 aacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt cctgataact
     5821 gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc agaaaggtga
     5881 gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat
     5941 gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc aaaattacgg
     6001 cagcgccaaa ataaaactca gccggtccca tagtaaaagc gaggatctga tcgcgattaa
     6061 tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg
     6121 tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc
     6181 tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata ctggcgataa
     6241 acacgattcc cgcaaccgca atgttgctac ttgccagcga cataattccg gaaagaatcg
     6301 tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg ttggctggta
     6361 acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta tgacgacgac
     6421 gcaggcgtag cgggatatgg caacgcgggc agagaccacg ctgatcagga tagccggtaa
     6481 aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg cgctgcggat
     6541 aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca
     6601 ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa tcctgtacct
     6661 ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac tctttaagtc
     6721 gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca aaccataaat
     6781 atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag accattgatc
     6841 ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa
     6901 tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac ggcataagca
     6961 acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa
     7021 tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg ggcaggctaa
     7081 acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc tcgccgattg
     7141 ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc
     7201 aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt gagcattaac
     7261 gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa gtgattaaat
     7321 aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc tgatggcggg
     7381 agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac
     7441 tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac tcggaccatt
     7501 tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt
     7561 tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc atattgcctg
     7621 tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc agattattgg
     7681 tgttctcgac atcgatagta ccgtcttcgt tcgctttaca gacgaggacg agcaaggctt
     7741 acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca aaaaattctt
     7801 tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg tcattataat
     7861 gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa atttcatgga
     7921 aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg aacgttttcc
     7981 ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt ttcaggattt
     8041 ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat ccgctttacg
     8101 tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga
     8161 tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg ctcgcaagca
     8221 gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg
     8281 cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg
     8341 tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt
     8401 agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc cggtttctga
     8461 catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga
     8521 tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc
     8581 tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca
     8641 tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag accttcgctg
     8701 tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg cagcatgcga
     8761 cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag ttcgacctcg
     8821 atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat tacagccaca
     8881 atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg
     8941 aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg caaaagcgcc
     9001 gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat ttcaccggca
     9061 acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag gctgagttga
     9121 acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg acaggaaaaa
     9181 cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt cgtcgtctgg
     9241 cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg cgtgaaatcg
     9301 atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact gaaatgagtt
     9361 tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc gttatcaatt
     9421 cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt gacaaaattg
     9481 tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt cttgatgatg
     9541 tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt ttacctgctg
     9601 gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt ctcgaagacc
     9661 gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc
     9721 cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa ctggaaaaac
     9781 agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag
     9841 cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc cgcgataaca
     9901 acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa ggcccgctgg
     9961 tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg
    10021 attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat
    10081 accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt
    10141 ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg caacgtaaag
    10201 gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat
    10261 tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg aaatcaggag
    10321 atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc gcgaaagatc
    10381 ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca
    10441 atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc
    10501 gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg aagaagctgg
    10561 atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg gtgaatatcg
    10621 cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaaaccgct cccgtcaagt
    10681 aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag atgccgtaaa
    10741 tcagatgcta caaaatgtaa agttgtgtct ttctggtgag ttacgcacta tccagacttg
    10801 aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt taaactgagg
    10861 taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt
    10921 cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc aggggctgat
    10981 gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg
    11041 gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg
    11101 ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt
    11161 ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc
    11221 tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag ccgaggcggt
    11281 aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga cgctgattca
    11341 gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg
    11401 ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc tgatctactt
    11461 tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta tcaccatgtg
    11521 gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg ttggtcgcga
    11581 gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag aagcaaccag
    11641 catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt tcatgactca
    11701 cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga agtaatatga
    11761 agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg gctcgtggct
    11821 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
    11881 gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc agcgccgcgc
    11941 cactactctg acccagtaga cgagccgttc ctaacatgcc actggcacca ccgctacgtt
    12001 cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag ccaaatccag
    12061 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
    12121 ccagggaaaa aagccccgca gccatgatga acaaccctaa agcacccagc aatcctgcat
    12181 gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct aacggccacg
    12241 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc aggtaaaagg
    12301 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    12361 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    12421 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    12481 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    12541 cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg
    12601 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    12661 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    12721 agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt tgtggataga
    12781 tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa ccttgtatga
    12841 cacgcgcaag ggtgagcatt tgcagcgaat cagaaaggga gcagaacagt gaagacaaca
    12901 gaaaaacgac cagaccgcat tataaatacg tcgatagcca aacatatcgc ccagaaacga
    12961 aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc agatggaact
    13021 ggctggtgtg gcatgaaggt ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc
    13081 gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt atcgctgggg
    13141 caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt ggtgaataat
    13201 caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat tgtaactaac
    13261 cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac agttctgatt
    13321 tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat aaacagcctg
    13381 attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt gaagagcgcg
    13441 aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct
    13501 ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg gagaaatatt
    13561 ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc
    13621 gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact atccacctcg
    13681 gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg tacaatttgc
    13741 gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac
    13801 tgctggcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg gagtataaac
    13861 gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg gaccaggtgc
    13921 gcgagcaggg gtatggcgaa gataacgaag agcaggaaga agggctgcga tgcattgcgg
    13981 taccggtatt tgatcgcttt ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt
    14041 tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca
    14101 aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa gaacctgtcg
    14161 gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga aggtgcgtta
    14221 tattaaacgc gcttgctata agaatatttt actcatgagt gagaatctgg ttatttatta
    14281 cccttaacca ttatcgacca cgatattgct tttacgtaac agtgggcaat ctgttatccc
    14341 caagaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg cagtcagata
    14401 ggtacattgc aaacctaatc ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc
    14461 gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa tgatgaatcg
    14521 cattacaacc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca
    14581 agctactcat tccgaaaaag cacgaatatc gacatagtta ggcgctgttt aactaacgca
    14641 tgctagttta atgacataag gtaggtgaag cggagattgg agtgaaaaag tttcgatggg
    14701 tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta ttcaacatga
    14761 tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag tttatcccgt
    14821 ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta gaatagcgcc
    14881 tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc
    14941 tttggttgta tcggtggtgg ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg
    15001 tgccagttca cggactaacg aacagattga actgcttgag gcgttgctgg atcagcaaaa
    15061 acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac gaaccagaga aagcagataa
    15121 aaagaccatt gagagtcaaa aatcggttga tgacgaagat attattcgcc tggtcgccga
    15181 acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc aattcatcct
    15241 gattatacgc actgtcatgg tgcaactgct tcaaattaaa actctggtct gacttcaaat
    15301 aattatcata ttatttattt gcgtaacggg atatcagaaa aagatatttt cctcattaat
    15361 ttattaggat gtttacatca gatttgtgat taagcgtggt attatttatt agcgaaacgt
    15421 ttctctcttg atttttttgc tcactcatca atttttctta ttttaaattt acaatccctt
    15481 tggggattga cttctcttta gggtaattga tagccgttaa ctgactgttt tatgagaaaa
    15541 agtgatataa ctttttattc attgcatagc agaaaatgtg atattgcacg cactatgtaa
    15601 taacttctcc cactggcctg gaacaactga acttattgaa ctatgttaga aactacgcca
    15661 gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg atatatcggc gcgtattccg
    15721 ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca
    15781 tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat aaaagatttc gggttaaacg
    15841 aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta aacacgttga
    15901 cgttgaatac cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt
    15961 tttatctatg ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa
    16021 gattaaaggt caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactccggc
    16081 tgatggcagc aaagatgtgt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac
    16141 tctggctgaa ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc
    16201 tgttaacgta acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc
    16261 tcgctataaa gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg
    16321 ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt atcctgccgg
    16381 agttgtcatg tcgttttcct gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag
    16441 ctatatctgt ccccaacgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct
    16501 gcccgttcag cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg
    16561 ccgcgcattc ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact
    16621 gcgggaacgg cttgatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta
    16681 ttacacacac gcatttgccg atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc
    16741 gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc
    16801 cagccaccgt ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc
    16861 gtgtaaagca gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac
    16921 gccgggaccg cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca
    16981 tgcacctcat gcagaacaac tggaaggttt tacattacag cagagtgcgg agttgtgtta
    17041 tccgatgcgt cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg
    17101 gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg actgccagac
    17161 ggactttaat attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat
    17221 ctggacgccg atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat
    17281 tgagccgata aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc
    17341 aatggccaat gcggtcgcga taatgttgac ctgaaggaaa gcaagaccaa cacccacagc
    17401 catggcatcc aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg
    17461 gcgcggctct tcatcttcat catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc
    17521 gaggaatatc agcagcacaa acgcaatcca gtgattccat tcaaggacaa accggctggc
    17581 taacatgccc attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag
    17641 gccggttcgc aatgcttcag aaaatttcgg tttatggagg ggggcacctt taccgattga
    17701 tgcagcaaat gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat
    17761 gacaacgtcc tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac
    17821 atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    17881 tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    17941 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    18001 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    18061 gattttcatt tatgtttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    18121 gataatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    18181 attcataagt tattgagttg ata
//
LOCUS       DADKXM010000075        18054 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.076, whole genome shotgun sequence.
ACCESSION   DADKXM010000075 DADKXM010000000
VERSION     DADKXM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.076"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..519
                     /locus_tag="JQR05_002500"
     CDS             <1..519
                     /locus_tag="JQR05_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY5324649.1"
                     /translation="PVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAID
                     DQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSS
                     GQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTADKPGDAHASGWTL
                     AAKRGRDAGGGK"
     gene            516..842
                     /locus_tag="JQR05_002501"
     CDS             516..842
                     /locus_tag="JQR05_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY5324650.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            852..1202
                     /locus_tag="JQR05_002502"
     CDS             852..1202
                     /locus_tag="JQR05_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY5324651.1"
                     /translation="MAISAGRLTQMISILNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1199..1645
                     /locus_tag="JQR05_002503"
     CDS             1199..1645
                     /locus_tag="JQR05_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAY5324652.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1642..1986
                     /locus_tag="JQR05_002504"
     CDS             1642..1986
                     /locus_tag="JQR05_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAY5324653.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            2053..2769
                     /locus_tag="JQR05_002505"
     CDS             2053..2769
                     /locus_tag="JQR05_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5324654.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            2775..3149
                     /locus_tag="JQR05_002506"
     CDS             2775..3149
                     /locus_tag="JQR05_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5324655.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            3245..3454
                     /locus_tag="JQR05_002507"
     CDS             3245..3454
                     /locus_tag="JQR05_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY5324656.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            3502..6561
                     /locus_tag="JQR05_002508"
     CDS             3502..6561
                     /locus_tag="JQR05_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY5324657.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERLRE
                     CMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQETFYAES
                     VLQEISKVLMNAAIVNGIRTAANSMSGAGGFIGSIGSWLGGAVANAKGGVYTSANLSA
                     YSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSP
                     VYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6554..6895
                     /locus_tag="JQR05_002509"
     CDS             6554..6895
                     /locus_tag="JQR05_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5324658.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            6895..7593
                     /locus_tag="JQR05_002510"
     CDS             6895..7593
                     /locus_tag="JQR05_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY5324659.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQSGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMTASFVLVTPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYMGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            7604..8347
                     /locus_tag="JQR05_002511"
     CDS             7604..8347
                     /locus_tag="JQR05_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5324660.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVLSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMVVT
                     GFYRVPLSSAQPGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWNTSAFTGIYNDLAAASACM"
     gene            8245..8925
                     /locus_tag="JQR05_002512"
     CDS             8245..8925
                     /locus_tag="JQR05_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY5324661.1"
                     /translation="MATTNAFCLASPPLEHICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRLQMNEGWYQIRIAGEDTAPEVVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSAMAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9166..12645
                     /locus_tag="JQR05_002513"
     CDS             9166..12645
                     /locus_tag="JQR05_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY5324662.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPHRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQMVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTSSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTLNSVIPPAVQHL
                     TVEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSHLVTTAITADTEHRFSGL
                     PLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPT
                     VQFEFWFSEKRITNTAQVEKSARYLGTGSQWTVSGPHIKPGKDFWFYVRSVNLVGKSA
                     FVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNE
                     ITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDT
                     DGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPP
                     TFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGK
                     AFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGAKHQNFNSPNQQSYWYSTCKLQ
                     VLKNGVEIFHEPATDVSRVFSSVIDMPAGQGHVTLTFNVSSAGANNWTPTTYISDLLV
                     VVMKKSTAGISIS"
     gene            12714..13337
                     /locus_tag="JQR05_002514"
     CDS             12714..13337
                     /locus_tag="JQR05_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY5324663.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTVHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVEMVVIDLAYEGSGSGDWRTDGFIVGIGYKF"
     gene            13402..16215
                     /locus_tag="JQR05_002515"
     CDS             13402..16215
                     /locus_tag="JQR05_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5324664.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATRATDAGDSARAANTSAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASVTAA
                     EKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAAS
                     SASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAA
                     TRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAN
                     GRVPSNRKVNGKALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYI
                     ALIGGAGYNVVSPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTY
                     DIYVEIGNYATSVNIHWDCTTNASVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTP
                     SDIGALPTTGGTISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTP
                     ENIGAFARRSTGAYADSDGAVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKA
                     HYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQG
                     QTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASST
                     DLGTKTTSSFDYGTKSSNATGDHNHSRGTMEITGTLGYFRSDSGNFYTASGAFTLGGS
                     APAHGFTGSQFTYGVPVNFNASRTWSGATSTTGNHAHSVPIGAHAHSVAIGSHGHTIT
                     VNAAGNAENTVKNIAFNYIVRLA"
     gene            16215..16799
                     /locus_tag="JQR05_002516"
     CDS             16215..16799
                     /locus_tag="JQR05_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5324665.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPLDIPAGFVAVFNSDESSWHLFEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIRDAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(16915..17163)
                     /locus_tag="JQR05_002517"
     CDS             complement(16915..17163)
                     /locus_tag="JQR05_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY5324666.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESANAWFVH"
     gene            complement(17225..>18054)
                     /locus_tag="JQR05_002518"
     CDS             complement(17225..>18054)
                     /locus_tag="JQR05_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5324667.1"
                     /translation="TKRYVLELLMDSDISAIKLSELTENDVIEHCRLRNNAGAGPATV
                     SHDVSYLGSVLDAAKPVYGINYTSNPAKSARPYLLKLGLIGKSNRRNRRPASDELDML
                     IEGLQQRSTHKCSKIPFVDILKFSVWSCMRIGEVCRLRWEDLDQEQKSILVRDRKDPR
                     KKEGNHMKVALLGEAWDIVQRQPQKSEFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDL
                     RREGASRLFEAGFSIEEVAQVTGHRSLNVLWQVYTELYPKSLHNRFEELQRSRNKIS"
BASE COUNT         4245 a         4395 c         5541 g         3873 t
ORIGIN      
        1 cctgttccgc gtgatggtcg tgactacgat ccggatgttc tgcagaaggc ggttctggat
       61 gcggtgagtg ccctgccggc tccgcaggac gggcgtgatg ccacggcact ggaaatactc
      121 cccgccattg acgatcaaaa atcctttccc cggggcacgt atgccacaca ccagggcgga
      181 ctctggcggg cgtatgaaaa aacgcacggg atgcggggat gggaatgcct ggttgacggg
      241 gtggcggata ttgacgtcag catgacgggt gaacggttgt tctctgtggt ggtccggcag
      301 agcagtggcc agcgtacgga aaaaacattt tccctgccgg tgatgctcta ccgcggtgtg
      361 ttcagagccg gtgaaaccta ccaccccggt gatacggtga cgtggggggg ctcgctgtgg
      421 cactgcaaca gtatgaccgc cgataaaccg ggggatgcgc atgcatctgg ctggacgctg
      481 gctgccaaac ggggacggga tgcaggaggc ggaaaatgac ggcattactg acactggaag
      541 agatcaaggc acatctgcgt gtcgaccatg acgcggatga tgacatgctg atggacaagg
      601 ttcgtcaggc taccgccgtg ctgctggcct acattcaggg cagccgggat aaagtgattc
      661 gtgaggacgg tgaactgatc ccgggcgagg cattaacccg gatgaagggg gctgccatgc
      721 gactgaccgg gatgctgtac cggaatccgg atcttgcgga gcgggaagaa ctgattcagg
      781 gggagctgcc gttttctgtt tccgtgctga tttacgattt gcgttgtccg acggtgttat
      841 gaggaggggg aatggcaata tctgcaggtc gtctgacaca gatgataagt attctgaacc
      901 cggtgttaac ccgtaatgct gccggagaaa tgacggaaga atgggtgtca tgcgggaaaa
      961 ttcatgcgga tatccgtggc aggagcagcc gggagcggat gcagtccggt gcggaaatgg
     1021 cgcaggcgga aatccgcatc tgggtgcgcg gtcagtccgg ccgggaaatc acggcagcgt
     1081 cacgacttca tgtgctgagt ggtccatggc gtgaccggat cctgaacgtt gtcgggctgc
     1141 ccgtgccgga tgcgaccggt gggcgtctgg aaattctctg tcggctggga ggggaaaaat
     1201 gatcgaaacc ctgctggatt tttcggggct ggaggacatc agccgcgatt tgcagcttct
     1261 gagtggtgcg gaaaataacc gggtgctgcg tgaggcaacc cgtgcggggg cgaatgtgct
     1321 gaaagaagaa gtggtgtcac gggcaccggt acgcaggggg aaactgcgcc gcaatgtggt
     1381 ggtcctttcc cggcgctccc gcgatggcgg gatggaatcc ggtgtccata tccgtggtgt
     1441 taatccggac accggtaaca gcgataacac catgaaggcg gataacccgc gcaatgcttt
     1501 ctactggcgg tttgtggaaa tggggaccgt gaatatgcca ccgcacccgt ttgtgcgtcc
     1561 ggcatttgat gtgcgcagtg aacaggcagc gcaggtggcg attgctcgga tgaaccgggc
     1621 cattgatgag gtactgagac gatgacggag gcggatttgt atcctcatct ggcgcatctt
     1681 gccggcgggc aggtgtaccc gtatgtggtc cccctgctgg atggcaggcc gtcggtggcg
     1741 cttccgtggg tggttttcag cctgatttca tcggtgtctg cggacgtgat gggcgggcag
     1801 gcggagtcct cagtgtcggt gcagatagac gtttatgccg ggactgtgac gcaggcgcgt
     1861 cagatacgtc aggacgcccg tgaagccata atgctgctgg ccccgggatc cgtcagtgaa
     1921 atgcaggact atattccgga aaaccgctgt taccgtgcaa ccctggagtt tcaggtcacg
     1981 gtgtgacttt ttcttttttt ctacaaaacc cataccccgc cgcgtgcggg ttttttatta
     2041 tcaggaggca gaatgtctgc tttgtatgaa cgctcacagc tgacgcaggt gatgatttca
     2101 tctgccccgg cgactgctga aactatggat aaggcggaat atctgcgcct ggactgcacc
     2161 atcaaggaag tccagttcac cgccggtcag aaacaggata ttgatgtgac cacgctctgc
     2221 tccacagagc aggagaacat caacggtctg ggggcgtcgt ccgagatttc catgtcgggt
     2281 aatttttatc tgaatcaggc ccagaacgcc ctgcgtgatg cctatgacaa tgacacggtg
     2341 tatgcgttta aggtgcagtt tccgtccggt aagggcttta agttcctggc ggaagtgcgt
     2401 cagcacacct ggtcatccgg taccaacggc gtggtggctg caacgttttc acttcgcctg
     2461 aagggtaaac cggtgtccta tgtggtaccg ctggcgtttg tgaaaaatct ggagaagaca
     2521 cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg gggaacgccg
     2581 ccttataaac acgcctggaa gaaggatggt cagccggtag agggacagac tactgacact
     2641 ttcagtaaag ccaatacgca gtcaggtgat aagggggctt atacctgcga ggtaacggat
     2701 tctgcagaac agccgcagag cattacctct gatgcgtgta cagtaacggt taatggtgcg
     2761 ggcggataag gcttatggca aaagatctga aaacactggc gctggccaga ctgtcagggt
     2821 tccgtcataa aacggtgaag gtgccggaat ggggaaatgt cagcgtggtg ctgcgggagc
     2881 cttcggcaga ggcctggtat ctgtggcagg aagtgctcaa tggtgatgga gaggatgacg
     2941 ataccctgtc ggtggtggcg aaaacccgcc gtaacctgga agcggatgtg acgctgttct
     3001 gcgatgtcct gtgtgatact gacctgcaac gggtgttcac tccggacgac cgtgagcagg
     3061 tgctggccgt ctatggtccg gtacatgccc ggttgctgcg tcaggcactg gaactgatcg
     3121 ctgatgcaga gtcggccaga aaaaagtagc ccgcccggaa attcgctttc tgatgcgact
     3181 tgcgctccgt ctggggcgca ccttatccga actgcggcac agcctgagtg cgagcgaggc
     3241 gatgatgtgg atggagtttg acagggtgtc cccgctgggt gatgagcgcg gggatatccg
     3301 taatgcacag atcgtgaaag cggttttcgg ggcacagggg atgaatgttg cactgaagga
     3361 cgccatgctc tgctggggcg aggatgagga taagccggag gtggatccgt ttgcggcgct
     3421 ggaagacgcg ctgagctttg cagcacagtc atgaatgatg agaaccgctg aggcggtttt
     3481 tttacgcccg gagaaaggtg aatggcgacg ttacgtgaac tgattatcaa aatttcggca
     3541 aattcacagt cattccagtc ggagatccag cgggcttccc gtatgggcag tgaatattac
     3601 cggaccctgc agaatggcgg gcgtcaggcc gctgcggcag cccgggagca gcgacgtgcc
     3661 ctggcagaac tgaacagcca gttgacggaa atccgcgctt cggctgtcgg aatggctggc
     3721 gcatttgccg gtgcctttgc caccggacac ctgatttcac tggctgatga gtggagttcc
     3781 gtgaatgccc gtctgaaaca ggcatcacag tcatccgatg aattttcgtc atcacagaaa
     3841 gtgctgatgg atatcagtca gcggacaggt accgcttttt cggataatgc ggccctgttt
     3901 gcccgttcgg ctgcctcgat gcgtgaatat ggttacagtg ccggtgatgt actgaaggtg
     3961 acggaggcca tttccacggg gctgaaaatc tccggtgcca gtacggctga ggcgggttcg
     4021 gtgatcaccc agttcagcca ggcgctggca cagggtgtgt tgcgcggtga ggaatttaat
     4081 tcggtcaatg aaagtggtga ccggatcatt cgtgcactgg ctgcaggcat gggcgtggcc
     4141 cgtaaagatc tgaaggcgat ggcggacgat ggtcaactga cggcggataa agtcgtgccc
     4201 gcgttaatca gccagctggg gacattgcgt gatgaatatg ccgccatgcc agaaacggtc
     4261 tctgacggga tcacaaaggt ggaaaacgcc tttatggctt gggtgggcgg tgcgaatgag
     4321 gccagcgggg tgacaaaaac gctctccggc atgctgaacg gtgttgccgg acagattgat
     4381 aatgtggcaa cagctgtggg tgcgctggtt gccgtcgggg tggcccggta ctttggcaat
     4441 atggcctccg gagcgatgtc tgccacggca ggacttgtga cggctgcacg taatgaagtt
     4501 gcactggcgg aagcacagtt caggggaacg cagattgcca cggcgcgggc aagggcagcc
     4561 gtgtaccgtg ctcagcaggc cgtggcggca gcccgcggga cggagatgca gattgctgca
     4621 gaggcccgtc tggcggccac acaggaacgc ctgaacagaa atattgctgc cagaagcgcc
     4681 gcccagaatg cgctgaacag tacaacggcg gtgggctcac gtctgatgag cggtgcgctg
     4741 gggctggttg gtggcgtacc cggactggtg atgctggggg ctgcagcatg gtacacgctg
     4801 taccagaatc aggagcaggc cagggagtct gcgcgccagt atgcactgac gatagatgaa
     4861 atcgcgcata aaacgccgtc aatgtctttg cctgaagcct cagataatga aggacgaaca
     4921 cgggcggcgc tgacagagca gaaccggctg attgatgaac aggccagtcg ggtgaaatcc
     4981 ctgcaggaaa aaatcgcagg atatcagtat gttctggcga acccgggctg gacgaccggt
     5041 gacggattca tgataaacca tctgacctcg gtgaagaccg taacggaagg gcttgctcag
     5101 gcaacagagc agcttgccgt tgagcagtcc cgtctggcac agatgcagga aaaagcgcag
     5161 tccattcagg atgtgcttgc cgggctggaa gaccgtcgtg tggcgttaat tcgtcagcag
     5221 gcggcagagc agaataaggt gtaccagtcc atgctggtta tgaacggtca gcatacggaa
     5281 ttcaaccgtc tgctggggct gggtaatgaa ctgcttcagc agcggcaggg actggtgaat
     5341 gtgccgttac ggctgccaca ggccactctg gatgataaac agcagagtgc cctgacaaaa
     5401 acagagcgtg agctggccct gtccagactg aaaggggaag aaaaagagcg tgtccgactg
     5461 gggtatgcgg cggatgacct cggttttgtg ggtgatccgt atcaggaggc gagacaacgt
     5521 tatatcagta atgccctgga agcctggcgc aataacgagg cgaataaacc caaatcccgg
     5581 ggtggaaaat cagagacgga aaaagcggaa gacagttttt cccggctgct gaagcagcag
     5641 aaagagcaat tggcacttgc ggggcagaat acagagctgg cgaagctgaa ataccagact
     5701 gcgcagggcg aactgaaaac cctgacggag atgcagaagc aggaactgct gcgcaatgcg
     5761 gccctgattg accagcaaaa aatccgggag cagttgcggt cccgggaaga gaccctgaag
     5821 aatgataatg tggctgcgcg tgcatcaaat gaagcggaac tgctggggta cgggcaggga
     5881 gagcggctcc gtgaatgcat gcgggagttg cagcagatcc gcgacagttt ccgccagaag
     5941 gatgcggacc ttcagtctca gtatcagacc ggggatatca gtgaggattt ttacagacag
     6001 gctctggcac agaacgcgca gtatctgagt gaacgtctta aagaccagga aactttttat
     6061 gccgaatcag tgctgcagga aatatcaaaa gttcttatga atgccgcgat tgtcaacggg
     6121 atcaggacgg cggcaaacag tatgtccgga gcgggaggat ttatcggcag tattggtagc
     6181 tggctgggcg gtgcggtggc caatgcaaaa ggcggcgtgt atacctcggc aaacctgagt
     6241 gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa aggggcggga
     6301 ctgatggggg aggccggtcc tgaagccatt atgcccctga cccgggcggc ggatggctcg
     6361 ctgggtgtgc gagcggtggg cagtatgaac ggcagtgcag gtctggtgta ttccccggtc
     6421 taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga agctgcgggc
     6481 agccttgtgc aactgattga ccagcgggtg caggcggtga tgctgtccat gcgacgtgac
     6541 ggaggaatgc tgagtggctg agataaaaac gctgcatctg gtcccgcgtg aagggatgca
     6601 ggtgagtgag aaaccgtcgg tggcgagggt acggtttggt gacggttatg aacagcgccg
     6661 cccgacggga cttaatgccc gactgaagac gtttcaggcg gtgttccggg tgacggatga
     6721 ggcgacccgg cgatggctgg aagagttttt atcgtggcat ggtggttacc gtgccttttt
     6781 gtggcgaccg ccgaaacata accggacggt gagggtggta tgccgggagt ggagcgtcac
     6841 agataacgcc aggtacagtg atttcagttg tacgattgag caggtggtga actgatgcag
     6901 gatattcacg aagaaagtct gaacgagtcg gttaaatcag agcagtcacc gcgggtggta
     6961 ctctgggaaa tcgacctgac ggtacagagc ggtgagcggt attttttctg taatgagctg
     7021 aatgaaaaag gggagccggt cacctggcag gggcgtaagt atgaggcgta cccgattgat
     7081 ggcagcggct ttgaaatgaa cgggaaaggc agcagtgccc gcccgtcgct gacggtgtcc
     7141 aatctgtttg gccttgtcac cgggatggcg gaggatttgc agagtctggt gggtgccacg
     7201 gtggtccgtc gccgggtgta tgcgcgtttt ctggatgcgg tgaattttgt ggcgggcaat
     7261 ccggaagcgg acccggaaca ggaactgagc gaccgctggg tggtggagca gttatcagag
     7321 ctgacggcca tgacggcctc gtttgtgctg gtgacaccga cggagacgga cggggcgctg
     7381 tttcccggtc gcatcatgct ggcgaacacc tgtatgtgga cctaccgctc tgatgagtgt
     7441 ggttacatgg gcggggctgt ggcggatgag ttcgataaac ccaccacgga tatccgtaag
     7501 gacagatgca gcaagtgcat gcgcgggtgt gaactgcgca ggaatgtcgg caattttggc
     7561 ggtttccttt ccattaataa actttcgcag taaatcccgg tttatgacac agactgaatc
     7621 agcgattctg gcacacaccc ggcgatgtgc gcctgcggag tcttgcggct tcgtgataag
     7681 cacaccggag ggggagcggt atctgccgtg tgtgaatatc tccgcagagc cggaggcgta
     7741 ttttcgtatc gcaccggaag actggctgcg ggcagagatg cagggggaga ttgtggcact
     7801 ggtcctcagt catcccggtg agctgccctg gctgagcgag gctgaccggc ggctgcagat
     7861 aaaaagcgca ctgccctggt ggctggtctg ccggggggaa attcataaat tccgctgtgt
     7921 gccacatctg acaggacggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     7981 ggatgcatac catctggcgg gaattgatat gccggacttt catcgcgagg atgactggtg
     8041 gcgcaacggc cagaacctgt acctggacaa tatggtggtc accggctttt accgggtgcc
     8101 cctgtcctct gcacagccgg gcgatatcct gctgtgctgc ttcggcgcat cggtggctaa
     8161 tcatgccgcc atttactgcg gcaacggtga actgcttcac catattcctg aacaactgag
     8221 taaacgggag aggtattccg aaaaatggca acgacgaacg cattctgtct ggcgtcaccg
     8281 ccactggaac acatctgcct tcacggggat ttacaacgat ttggccgccg cctcagcctg
     8341 tatgtgaaca cggcagcgga agccattcgc gccctgtcga tgcagatgcc gggctttcgc
     8401 cttcagatga acgaaggctg gtaccagata cgtattgccg gtgaagacac ggcaccggag
     8461 gtggtgtacg cccgccttca cgaacagctg ggtgagggaa cggtcatcca cattgtgccg
     8521 cgactggccg gggccggaaa gggtggactg cagattgtgt tgggggcggc agccatcgtg
     8581 ggctctttct tcactgccgg ggcatcaatg gcgttatggg gttcagccat ggcagccggt
     8641 ggtttttctg ccaccacgat gctgttttca cttggagcca gcatgattct gggcggtgtg
     8701 gcccagatgc tggccccgaa ggcaaaaaca ccggattacc gcgcaacgga taacggcaga
     8761 cagaacacgt acttttcctc gctggataac atgattgccc aggggaaccc gatgccggtg
     8821 ccttacgggg aaatgctggt tggctcccgc cgtatatccc aggacatcag cacccgtgat
     8881 gaaggcgggg gcggaaaggt cgtggttatc gggcggcagg gataaaacat aaaaaaatcc
     8941 cgcagtgatc gcggagctgc ggggacagac aaatgaagat caatgttaag gagttgtttt
     9001 tgttactcgg gcaaaaaaac actaacgcag cgaaattata agcgccacag tcagtgtgtg
     9061 aaaatgtgaa gatattcaga aattttattc cgtcatgacg caggcacccg gtgaggtgcc
     9121 tgttgttttt gtgagtgaac aattatcacg gtaagaggtg atgtaatggg caaaggtggc
     9181 ggcagggcgc acacaccgcg tgaggcgaaa gacaatctca aatccacgca gatgatgagc
     9241 gtgattgatg cgattggtga gggaccgata gaaggcccgg tgaaaggcct gcagagtatt
     9301 ctggtgaaca aaaccccgct gacggacacg gacggtaatc ccgtgataca cggtgtgacc
     9361 gcggtctggc gtgccgggga gcaggagcag acaccaccgg aaggctttga gtcctctggc
     9421 tctgaaactg tactgggtgt cgaagtgacc agggcaaaac cggtaacacg caccattacg
     9481 tcagcgaaca ttgaccgcct gcgggtgacc ttcggggtgc agtcactggt ggagaccacg
     9541 tcaaagggtg accgtaatcc gtcctctgtc cgtctgctga ttcagttaca gcgtaacggt
     9601 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcaca gttccttgcg
     9661 tcggtgattc tggataatct gcctcaccgc ccctttaaca tccggatggt cagggagacg
     9721 gcggacagca ccacggacca gctgcagaac agaacgctgt ggtcgtcata caccgaaatc
     9781 atcgatgtga aacagtgcta cccgaacacg gccattgtgg ggatgcaggt ggatgcggag
     9841 cagtttggtg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggtg
     9901 ccgtcaaact atgacccgga aaaacgcacg tacagtggta tctgggacgg cagtctgaaa
     9961 ccggcataca gcaacaatcc ggcctggtgt ctgtgggaca tgctgactca cccgcgctac
    10021 ggcatgggaa aacgtctggg ggcggcggat gtggacaagt gggcgctgta tgccatcggg
    10081 cagtactgcg accagatggt gccggatggc ttcgggggca ccgagccgcg gatgaccttc
    10141 aatgcgtacc tggcacaaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
    10201 atgcgatgta tgccggtatg gaacggtcag acgctgacgt tcgttcagga ccgcccgtcg
    10261 gatgtggtgt ggccgtacac cagcagcgat gtggtggtgg atgataacgg cgtggggttc
    10321 cgctacagct tcagtgccct gaaggaccgg cacacggcgg tggaggtgaa ttacaccgac
    10381 ccgcagaacg gctggcagac ctccacggaa ctggtggaag acccggaagc catactgcgc
    10441 tacgggcgca acctgctgaa gatggatgcg ttcggctgca ccagtcgcgg tcaggcccac
    10501 cgtgccggac tgtgggtgat aaagaccgga ctgctggaaa cgcagacggt ggatttcacg
    10561 ctcgggtcac aggggctgcg tcacacaccc ggtgacatta ttgaaatctg tgataacgac
    10621 tatgccggga ccatgaccgg cggacgtgtc ctgtccatcg atgccgccac ccgcaccctg
    10681 acgctggacc gtgaggtgac actgccggag accggtgccg ccacggtgaa cctgattaac
    10741 ggcagcggta agccggtgag tgtggacatc accgcacacc ccgcgccgga ccggatacag
    10801 gtcagtaccc tgccggatgg cgtggagaca tacggtgtgt ggggactctc cctgccgtca
    10861 ttgcgccgtc gcctgttccg ctgtgtctcc gtccgggaaa acacggacgg cacctttgcc
    10921 atcacggcag tgcagcatgt gccggagaaa gaagccatcg tggataacgg ggcgcacttt
    10981 gacggcgacc agagcggcac cctgaacagc gtcatccctc cggcagtgca gcacctgacg
    11041 gtggaggtga gcgcagctga cggccagtat ctggcgcagg tgaaatggga cacgccgcgg
    11101 gtggtgaagg gcgtgcgctt cagtctgcga ctgaccagcg gaagcggaga aggcagccat
    11161 ctggtgacca ccgccatcac tgcggataca gagcatcgtt tcagtggtct gccgctcggg
    11221 gaatacaccc tgacagtcag ggcaattaac agttatggcc agcagggcga accggccacc
    11281 accaccttcc ggattaacgc gccagcaaaa cccgccacca ttgagctgac gccggggtat
    11341 tttcagataa cggcggtccc acgtcttgcg gtgtatgacc cgacggtaca gtttgaattc
    11401 tggttctcag aaaaacgcat cacgaacaca gcacaggtgg aaaaatctgc ccgttatctg
    11461 gggaccggca gtcagtggac tgtctccggc ccgcacatta agcccgggaa ggatttctgg
    11521 ttttatgtac gcagcgttaa cctggtgggg aaatctgctt ttgttgaagc cagtggacgg
    11581 gccagcaatg atgcagaagg gtatctgggg ctgtttcggg aaaaaatagg aaaactgcat
    11641 ctggctcagg ggctgtggga gctgatagac aacagccagc ttgcggatga gatggcggag
    11701 atgaagacca ccatcaccga aacccgcaat gaaatcacac agacggtcag taaaacgctg
    11761 gaagaccaga gcgccaccat tcagcagata cagcgcgtgc agaaggacac aaatgatgac
    11821 ctggctgcgc tgtacatgct gaaggttcaa aaaacgaaag acggcattcc ctatgtggcc
    11881 gggattggtg cagggattga ggatactgat ggccagccac tgagcaacat actgctgctg
    11941 gctgaccgta tcgcgatgat aaatccggag aacggcaaca gcacgccgtt atttgtggcg
    12001 caggggaatc agctgttcat gaacgacgtg ttcctgaagc gactgtttgc ggtgagtatc
    12061 acgtcatccg gcaacccccc gacgttttcc ctgacgccgg aagggaagct gacagccagg
    12121 aacgcggata tcagcggagc aattaccgcg aataccggca cgctcaataa tgtcaccatt
    12181 aacgagaact gtgtcatcag agggaaactg tctgcaaacc agattgaagg cgacctggtg
    12241 aagacggtgg ggaaagcctt tccccggaat aacagttatg ccagcgggac ggtaactgtc
    12301 acagtttacg atgaccaggg attcgaccgg cagattatca ttcccccggt gctgtttcgc
    12361 ggggcgaaac accagaattt caacagcccg aatcagcagt cgtactggta ttccacctgt
    12421 aagctgcagg tgctgaagaa cggggttgag attttccatg aaccggcaac ggatgtcagc
    12481 cgggtgttct catcggtgat agatatgccg gcagggcagg gtcatgtcac cctgacgttt
    12541 aatgtgtcgt cggccggtgc gaacaactgg acgccgacaa cgtacatcag tgatttactg
    12601 gttgtggtca tgaaaaaatc cacggcaggg atcagtatca gctgacggtt tattaacccg
    12661 gacgggcacc tcaggaggtg ccttttttat tgactgaaaa caaagaggta atcatgcggc
    12721 atttatacgc aacgatatta ttgtttacta ccctgctggc agggattgcc tttcctgcac
    12781 aggctgaaag cggacacggt gcattttccg tgggatatgc tcaggttcac ccgggcggcg
    12841 taccggcatt gtccggtacc ggtgcgcgtg caggtgattt aaaagggatt aatgtgaaat
    12901 accgttatga gttcacggtt cacctgggcg gcattgtcgc gctgagttat gcatcggtga
    12961 agaaaagtga cacgatgaag acgggtgaaa ataccttcca ttatgaaagc ctgcgcggtc
    13021 gttatgtcag tctgatggcc ggccctgtct ggcagctcag tgagcgggtc agtctctatg
    13081 gcatggccgg gatggcgtac acccgctggt ctgacagtgt tcaggattac cggcgtgatg
    13141 aagtaaaacc ggggtatgtg aaggagacca ccaccgccag tgatggccat actgcgcgtc
    13201 atctgtcgcc ggcctggaat gccgggattc agttcagtcc cgtagagatg gtggttattg
    13261 accttgctta tgaaggttcc ggcagtggcg actggcgcac tgacggtttc atcgtgggta
    13321 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
    13381 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca
    13441 ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
    13501 gtggtgaaca cgctggcatc tgaaaatccg gatgaagccg ggcgttacag catggacgtt
    13561 gagtacggtc agtacagcgt tattctgttg gtggaagggt tcccgccgtc acatgccggg
    13621 accatcaccg tgtatgaaga ttctcaaccc ggtacgctga atgattttct tggtgccatg
    13681 actgaggatg atgtccgtcc ggaggcactg cgccgttttg aactgatggt ggaagaggtg
    13741 gcgcgtaacg cgtccgcagt agcacagaac acggcagccg cgaagaagtc agccggcgat
    13801 gccggcacat ctgccagtga ggcggcaacc cgtgcgactg atgctggaga ttcagcacgt
    13861 gcagccaaca cgtcagccgg acaggccgcg acgtcggctc aggaggcttt ttccagcgca
    13921 ggaacggcat cagcaaaagc ctctgaggca tcgaaaagtg ctgctgctgc agagtcctca
    13981 aaaagcgcgg cggccaccag tgccggtgca gcgaaaacgt cagaaacgaa tgcggcagcc
    14041 agcgttaccg ccgctgaaaa atcggcagaa agcgccgcat cgtccgcttc aacagccaca
    14101 acgaaggctg gcgaagccac tgaacaggcc agcgcagcag cgaggtctgc ttccgcagcg
    14161 aagacatccg aaacgaacgc gaaagcgtcg gaaacccgtg cagaatcctc aaaaacggct
    14221 gccgcatcat ccgccagttc ggcggcgtca tcggcatcat ctgcgtctgc ttcaaaagat
    14281 gaggcgacca gacaggcgtc agcagcgaag ggcagcgcca cgacggcatc cacaaaggcg
    14341 acagaggctg ctggcagtgc gacagcggca gcacagagca aaagtacggc agaatctgca
    14401 gcaacgcgcg ctgagacagc ggcaaaacgg gcagaggata ttgcatccgc cgtggcgctt
    14461 gaggatgcga gcacgacgaa aaaggggata gtccagctca gtagtgcaac caacagcaca
    14521 tctgaaacac ttgccgcgac accgaaggca gttaaagcag cgaatgacaa tgcgaatggc
    14581 agggtaccat ctaaccgaaa agttaacggg aaagcactga ctgcggatat aacattaaca
    14641 ccgaaagata ttggcacttt aaattcagta acgatctctt tctctggcgg ggcagggtgg
    14701 ttcaaactgg caacggttac catgccacaa gcgagttcca tcgtttacat cgcattgatt
    14761 ggtggcgctg gttacaacgt cgtctcccca cagcaggcag gcatttcaga actggttcta
    14821 cgagcaggca atggaaaccc caaagggatt accggagctt tgtggaagcg tacagccgtc
    14881 ggattaacga atttcgcctg gatcaacaca tccggcgata catatgatat ttacgttgag
    14941 attggcaatt atgcgacgag tgtaaatatc cattgggatt gtactacaaa tgcgtcagtt
    15001 tctatttata cctcgccaac atattcagcg agtaagcctt ccagcgttac cgatggtgtt
    15061 gtttatacga tgtatagctc acatcagaaa cctacaccat cagatattgg agcgctgcca
    15121 acgactggag ggactatttc aggtccgttg tctgttactg atgggatcac cggggcactg
    15181 aaggggaacg ccgataccgc gacgaaactt gcggcagccc caaaaattaa cggtgttaag
    15241 tttgatggct cggcggatat taacctcacg ccggaaaata ttggtgcatt tgcccgacgt
    15301 tcgacggggg cttatgcgga ttcggatgga gccgttccct ggaatgccga atcaggcgct
    15361 tacaatgtca cccgctctgg cgacagctat attctggtta acttctatac cggagtcgga
    15421 agttgccgga ccttgcagat gaaggcgcat tacagaaatg gtggtctgtt ctaccgttct
    15481 tcaagagacg gttatggttt tgaggaagac tgggcagaag tttatacctc gaaaaatctt
    15541 ccaccagaaa gctacccagt cggcgcacca atcccgtggc catcagatac cgttccgtct
    15601 ggttatgccc tgatgcaggg gcagactttt gacaaatctg cctacccgaa acttgcagcc
    15661 gcttatccgt caggcgtgat ccctgatatg cgtggctgga cgattaaggg caaacctgcc
    15721 agtggtcgtg ccgtattgtc tcaggaacag gacggcatta aatcgcacac ccacagcgcc
    15781 agtgcatcca gtacggattt ggggacgaaa accacgtcgt cgtttgatta tggtactaag
    15841 tcttcaaatg ccactggtga ccataaccac agccgaggca ctatggaaat taccggtact
    15901 ctcggttact tcagaagtga tagcggtaat ttctatacag caagtggagc atttacactg
    15961 ggtggctctg cgccagccca tggatttacg ggctctcaat ttacttatgg tgttcctgta
    16021 aactttaacg cttccagaac ctggtctggt gccacaagca ctacaggtaa ccatgcgcac
    16081 tctgttccaa ttggtgctca tgcgcactcc gttgcgattg gttcacatgg acacaccatc
    16141 accgttaacg ctgctggtaa cgcggaaaac accgtcaaaa acatcgcatt taactatatt
    16201 gtgaggcttg cataatggca ttcagaatga gtgaacaacc acggaccata aaaatttata
    16261 atctgctggc cggaactaat gaatttattg gtgaaggtga cgcatatatt ccgcctcata
    16321 caggtctgcc tgcaaacagt accgatattg caccgttaga tattccggca ggcttcgtgg
    16381 ctgttttcaa cagtgatgag tcatcgtggc atctctttga agaccatcgg ggtaaaacgg
    16441 tttatgacgt ggcttccggc gacgcgttat ttatttctga actcggtccg ttaccggaaa
    16501 atgttacctg gttatcgccg gaaggggagt ttcagaagtg gaacggcaca gcctgggtga
    16561 aggatacgga agcagaaaaa ctgttccgga tccgggatgc ggaagaaaca aaaaacaacc
    16621 tgatgcaggt agccagtgag catattgcgc cgcttcagga tgctgcagat ctggaaattg
    16681 caacggagga agaaacctca ttgctggaag cctggaaaaa gtatcgggtg ttgctgaacc
    16741 gtgttgatac atcaactgca cctgatattg agtggcctac gaaccctgtc agggagtaat
    16801 cattgggatt atgccgcagc acgtcttaag caagaacgtg ctgcggttgg atggtgaact
    16861 ttcgatagtg cgagtattga atgatttcca gccgttaccg attttacgtg ttaattagtg
    16921 aacaaaccac gcgttagcag actcccaggt atctttcaga gtttcctgaa caaaagtttt
    16981 agctgaatct ttatcggcgg tgcgcgtaac agaaaggcca tcgttgctgg tagcttttac
    17041 gatcacctct acatcgtcat aacgtttact gatgcgtcgg gttaattctt cctttaacgc
    17101 atccacagca ccggttggca ttttagtcat tttttctttg gctatgcaga tttcaatacg
    17161 cataaaagtc cctctatact gtgtttgtat acagtattat ttttaactgt atggataaac
    17221 agtgtcaaga gatcttattt ctgctccttt ggagctcttc aaaacgatta tgtaaagatt
    17281 tcggatacag ttcggtatat acctgccata gcacgtttaa tgaacgatgc cctgtaacct
    17341 gggctacttc ctcaatacta aaaccagcct caaataagcg acttgcccct tctctacgca
    17401 aatcatggta tcgcagatcc ttaataccta atttgcttct taccctctga aatcccgcag
    17461 taacagaagt gctgttatat ggaaaaatga attccgattt ttggggctgt cgttggacga
    17521 tatcccaggc ttccccaagc aaggctactt tcatgtggtt gccttccttt ttgcgtggat
    17581 ctttcctgtc tcttacgagt atagattttt gttcctggtc gagatcttcc catcgtaacc
    17641 ggcatacttc tccgattcgc atacaggacc acacagaaaa tttgaggata tcaacgaacg
    17701 gaatttttga gcatttatga gtagatcgtt gttgaaggcc ttcaatgagc atgtccagtt
    17761 catcagatgc tggtctacga ttacgacggt ttgatttacc aatcaaacca agtttaagta
    17821 ggtatggacg agcacttttc gccgggtttg atgtgtaatt aattccgtat acaggtttgg
    17881 ccgcatccag aacactgcca agataactaa catcgtggct gactgttgct ggacctgcgc
    17941 cagcgttgtt tcttagcctg caatgttcaa ttacgtcatt ttctgtcagt tcagatagtt
    18001 tgatcgcgga gatgtcacta tccataagca gttccagcac atatctttta gtac
//
LOCUS       DADKXM010000076        17876 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.077, whole genome shotgun sequence.
ACCESSION   DADKXM010000076 DADKXM010000000
VERSION     DADKXM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.077"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..648)
                     /gene="yihS"
                     /locus_tag="JQR05_002519"
     CDS             complement(<1..648)
                     /gene="yihS"
                     /locus_tag="JQR05_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY5324668.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRV"
     gene            complement(665..1543)
                     /gene="yihT"
                     /locus_tag="JQR05_002520"
     CDS             complement(665..1543)
                     /gene="yihT"
                     /locus_tag="JQR05_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY5324669.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(1567..2463)
                     /gene="yihU"
                     /locus_tag="JQR05_002521"
     CDS             complement(1567..2463)
                     /gene="yihU"
                     /locus_tag="JQR05_002521"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY5324670.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVARTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            2631..3527
                     /gene="yihV"
                     /locus_tag="JQR05_002522"
     CDS             2631..3527
                     /gene="yihV"
                     /locus_tag="JQR05_002522"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY5324671.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITSQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3561..4346
                     /locus_tag="JQR05_002523"
     CDS             3561..4346
                     /locus_tag="JQR05_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5324672.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            4445..5044
                     /gene="yihX"
                     /locus_tag="JQR05_002524"
     CDS             4445..5044
                     /gene="yihX"
                     /locus_tag="JQR05_002524"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY5324673.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            5038..5910
                     /locus_tag="JQR05_002525"
     CDS             5038..5910
                     /locus_tag="JQR05_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY5324674.1"
                     /translation="MLKTIQDKARHRTRPLWVWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            5907..6344
                     /gene="dtd"
                     /locus_tag="JQR05_002526"
     CDS             5907..6344
                     /gene="dtd"
                     /locus_tag="JQR05_002526"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY5324675.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            6389..7330
                     /gene="fabY"
                     /locus_tag="JQR05_002527"
                     /gene_synonym="yiiD"
     CDS             6389..7330
                     /gene="fabY"
                     /locus_tag="JQR05_002527"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY5324676.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            7746..9561
                     /locus_tag="JQR05_002528"
                     /pseudo
     CDS             7746..9561
                     /locus_tag="JQR05_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10239..10457
                     /locus_tag="JQR05_002529"
     CDS             10239..10457
                     /locus_tag="JQR05_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY5324677.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            10675..10917
                     /locus_tag="JQR05_002530"
     CDS             10675..10917
                     /locus_tag="JQR05_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324678.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(11247..12176)
                     /gene="fdhE"
                     /locus_tag="JQR05_002531"
     CDS             complement(11247..12176)
                     /gene="fdhE"
                     /locus_tag="JQR05_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY5324679.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(12173..12808)
                     /gene="fdoI"
                     /locus_tag="JQR05_002532"
     CDS             complement(12173..12808)
                     /gene="fdoI"
                     /locus_tag="JQR05_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY5324680.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTNAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(12805..13707)
                     /gene="fdxH"
                     /locus_tag="JQR05_002533"
     CDS             complement(12805..13707)
                     /gene="fdxH"
                     /locus_tag="JQR05_002533"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY5324681.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(13720..16770)
                     /gene="fdnG"
                     /locus_tag="JQR05_002534"
     CDS             complement(13720..16770)
                     /gene="fdnG"
                     /locus_tag="JQR05_002534"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(16183..16185),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY5324682.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTETYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            16964..17797
                     /gene="fdhD"
                     /locus_tag="JQR05_002535"
     CDS             16964..17797
                     /gene="fdhD"
                     /locus_tag="JQR05_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY5324683.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         4361 a         4357 c         4659 g         4499 t
ORIGIN      
        1 aacgcgataa tgattatttc tggcgacgtc gtggataatc acggaagcca cgcgaatcgc
       61 gcgatccagc cattttttgt cgtgagtgac gtcataaaca atcaagaaag cttccaccgc
      121 gtgcatattg gcattgccgc cgcggtactc ttcggttttg ctgaaggctt cgtcccagga
      181 ttccaggcac atctgctctt cttcgctcca gaaatatttc tcgataatct caatggtgta
      241 atcgagcagc ttgcgcgctt ccgggtgacc cgttgtgacg gcgctggcgg cacccagcag
      301 agcaaagaag tgttgatagc cctgtttgga ggcatccacc acgccctcgt cattcacgca
      361 ggcataccag ccgccatatt ttttatcacg cagtgcgccg ttcatggctt taataccgtg
      421 atcaaccaac gcgtaagcgc caggtcgacc catcgccgca gcaacggaat aaacgtgcaa
      481 catacgagct gtgatccaca gatgggtgcc catctcttct ttgatttgcc ctttattgcc
      541 taaccagcca aaaccagtcg gcactacgga atttttacca aaatcaaaga tgcggtcggt
      601 ttcctgttcc agccaacggt tgtggcttag ggtgttaaac catttcattc cttttatcct
      661 catcttagcg gcgtttagcc atcatttcgt cgacgatatc gccaagttgt tgtaatttcg
      721 gtgcgcaaac gtcacggagc atcagctcgt tgtctggtaa accgacgacc gatgcccaga
      781 ctgcacggcc tgccaggaat cccgatgcgc ccgccgtcat tgccacgcgt acggcacgcg
      841 ggaacagttt ttcgtcgaca ccggaagaga ggatcaccca tggcatattg atatggtcat
      901 tcagacgttg tgaagcacag agaagctctt gttgcggacc tttgccataa agcggcattt
      961 caactttgta gaggtcagcg ccactgtcgc ccagctcttt ggcggcatcg atgatcgctt
     1021 gttcgcgatc gaatttatcg ccacgacgcg gtgggcggac gactggctca atgatgctta
     1081 ccagaccgtg tgagtggcac agttcgttga actctttcac catatccaga cgttgctgcg
     1141 catcttcatc actacgccac agcaccagca gttttaaggc tttgccaccg tcttgtttga
     1201 tctgtagcgg attgattttg cggtcaataa ccacgctatc gaccggaata ccgttgccag
     1261 gaatgaactc atcggcggcg acaatcatgg cgcaactttt ggcaatcgcg ttttgctcaa
     1321 ccacctggcg gtagcagaat tgttgatcta ccagaatcgc cgaggcataa ggcgagaggg
     1381 cctttgcagc gttaactttg aaatcagtta aaacgctatc ggctaccgga gcgggtgccc
     1441 cagccgcggc aaacatcatg cgcatggctt cgcgctgatc gaccgccagc atggcaaaac
     1501 cgcccgatgc gcgcgtaatg tcgttgatgg tgtacttatt cattcattta ttcctttatc
     1561 cagtcgttac atttttactt tggcagtcat cccggcactg acacggacct gttccagaat
     1621 ggcggaccag tcctggcgac cgcgacccgc tgcgcgcgcc tggctataaa cctcccgtga
     1681 ggcggccccc agcggcattg gcacatgcag ctggttggcg acatcaaggg cgatgccaag
     1741 atccttatgg gcaagatcga tcatgaaggc gggagaaaga tctccgctga ggactttgtt
     1801 tggccaggaa gtggtgaagt gacctttacc ggcggcggta ccgctcatca ctttgacggc
     1861 aacatcgaag ggaagattca gggcttcgca caaaacggcg gcttctgccg aaagcgcatt
     1921 gagcgcgatg ctcatgtagt tgttgatgag cttaacgcgg atccccatgc ccggaccgcc
     1981 agcgttgatc aactcactgc ccatcgccat caggatcggt gtggcacgtt caacttgttc
     2041 agcggtgccg ccagccagca gtaacagagt accggtaatg gcatttgcag aagtacgggc
     2101 taccggaaca tccatcatgc tgaagccttt ggcttgcata tcggcaatca atttatcggt
     2161 ttgcagcgga tggatggtgg acatatcaat gaccagcgca tcggtagata agccttcgca
     2221 aacgccgttt tcaccgaaca acacgttgcg caccagatcg ccattcggca acatggtaat
     2281 gataaattcg gcatctttag cggcctgcgc cgggttggcg gcgggagtcg cacctttgtc
     2341 taccagatgc cgcacagctt cggcgttcac atcaaagacg cgaagttgat gcccttgctg
     2401 caataaattg ctcgccattg gcgaacccat ttgtcctaaa ccgataaatg cgattgctgc
     2461 cataaccctc tcctgaatac agtgatgtca ctttttgtca tttatgacat gctttgcttg
     2521 tctgtttttg atcgcatttg taatttatcg tcaaaaaatt gacagccgtc actttttaaa
     2581 caattggtga aattaaaatg aacgcatccc aaaatgttta aggaatgacc atgattcgtg
     2641 ttgcttgtgt aggtataacc gtgatggatc gcatctatta cgtggaaggg ttaccgacgg
     2701 agagcggtaa atacgtggcg agaaattata cggaagttgg tggcgggcca gcggcgacgg
     2761 cagcggttgc ggcggcaagg ctgggggcgc aggtcgattt tattggtcgc gtaggtgatg
     2821 acgacgcggg caacagcctg ctggcagagc tggaatcctg gggcgttaac acccgttaca
     2881 ccaaacggta taaccaggcg aaatcttcgc aatccgccat catggtggat accaaaggcg
     2941 agcggataat cattaactac cccagcccgg atctgctgcc tgacgcagag tggttggagg
     3001 aaattgattt ctctcagtgg gatgttgtgc tggcagatgt acgctggcac gacggcgcta
     3061 aaaaagcctt caccctggcc cgtcaggcgg gtgtgatgac cgttctggac ggggacatta
     3121 cgtcgcagga tatcagtgag ctggtggcat taagcgatca cgcggccttt tcagaaccgg
     3181 gtctggcgcg cttaacgggc gtgaaagaga tggccagtgc gctaaaacag gcacaaacgc
     3241 tcacaaatgg tcatgtctat gtgacccagg gtagcgcagg ctgcgactgg ctggaaaatg
     3301 gtgggcgtca gcatcaaccg gccttcaaag ttgatgtggt agataccaca ggtgcgggtg
     3361 atgtttttca cggcgctttg gcggtggcgc tggcaacaag tggggattta gcggagtcag
     3421 tccgcttcgc cagcggtgta gcggcgttaa aatgcacacg tcccggtgga cgagccggga
     3481 tccctgactg tgatcaaacc cgatcttttt tgtcactttt tgtataaaat gccagggtga
     3541 tggtttttcg aggaattttc atgagcctta ccgaactaac cggtaacccg cggcacgacc
     3601 aactcctcat gctgatcgcc gagcgtgggt atatgaatat tgatgagctg gcaaatctgc
     3661 tggatgtctc cacgcagacg gtccgccggg atattcgtaa attaagcgag caaggcctga
     3721 ttacgcgcca tcacggtggc gcgggtcggg cttccagcgt cgttaatacg gcgttcgagc
     3781 agcgtgaggt ttcgcaaacc gagaaaaaaa aagcgattgc cgaagcggtg gcagactata
     3841 ttcctgatgg ttcaacaata tttatcacca ttggtacgac tgttgagcat gttgcccggg
     3901 cgttacttaa ccataatcat ttgcggataa tcaccaacag cctgcgtgtg gcgcatattc
     3961 tttaccacaa cccgcgcttt gaagtgatgg tgcccggcgg tacgttgcgc tctcataata
     4021 gcgggatcat tggcccttca gcggcgtcct ttgtggctga ttttcgtgct gattatctgg
     4081 taacaagcgt tggggcgatt gagagcgatg gcgcgttgat ggagtttgat gtaaacgaag
     4141 ctaacgtggt gaaaacgatg atggcgcacg cgagaaatat tctgctggtc gccgatcaca
     4201 ctaagtatca tgcttcggcg gcggttgaaa ttggtaacgt ggcacaggtc actgcgctct
     4261 ttaccgacga gctgccgccc gctgcgctaa aatcacgctt acaagacagc caaattgaaa
     4321 tcattcttcc ccaggaagac gcgtagattt ttgtgaccgt taacctggct tcataccttg
     4381 ccacatagcc aaacccatcc tttcccgcta cagttaattt cttgtggcgc gaaaggaggc
     4441 aaaaatgctc tatatctttg atttaggtaa tgtgattgtc gatatcgact ttaaccgtgt
     4501 gctgggagcc tggagcgatt taacgcgtat tccgctggca tcgcttaaga agagttttca
     4561 tatgggggag gcgtttcatc agcatgagcg tggggaaatt agcgacgaag cgttcgcaga
     4621 ggcgctgtgt catgagatgg ctctaccgct aagctacgag cagttctctc acggctggca
     4681 ggcggtgttt gttgcgctgc gcccggaagt gatcgccatc atgcataaac tgcgtgagca
     4741 ggggcatcgc gtggtggtgc tttccaatac caaccgcctg cataccacct tctggccgga
     4801 agaatacccg gaaattcgtg atgctgctga ccatatctat ctgtcgcaag atctggggat
     4861 gcgcaaacct gaagcacgaa tttaccagca tgttttgcag gcggaaggtt tttcacccag
     4921 cgatacggtc tttttcgacg ataacgccga taatatagaa ggagctaatc agctaggcat
     4981 taccagtatt ctggtgaaag ataaaaccac catcccggac tatttcgcga aggtgttatg
     5041 ctaaaaacca ttcaggacaa agccaggcac cgtacccgtc cactatgggt ctggctaaaa
     5101 ctactctggc aacgtattga tgaggacaac atgacaaccc tggcaggtaa ccttgcctat
     5161 gtgtcgttgc tctcattagt gccgctggtt gccgttgttt ttgcgctttt cgccgctttt
     5221 cccatgtttt ccgacgtcag cattcagtta cgtcacttta tttttgccaa ctttctgcct
     5281 gctactggcg atgttattca gcggtatatc gaacaatttg ttgctaattc caacaagatg
     5341 accgccgttg gggcgtgcgg gctgatcgtc accgcgttat tgttgatgta ctccatcgat
     5401 agcgcgttga ataccatctg gcgcagtaaa cgagcgcgac caaaaattta ctcgttcgcc
     5461 gtgtactgga tgattttaac gctggggccg ctgctggcag gggccagtct ggcgatcagt
     5521 tcctatttgc tctccctgcg ctgggcgagc gatctcaata ctgtcatcga caacgtgctg
     5581 cgtatttttc cgttgctgtt gtcgtggatc tccttctggt tgctgtacag cattgttcct
     5641 accatccgcg tacctaaccg cgacgcgatt gtcggcgcgt ttgtcgccgc actcctgttc
     5701 gaagcaggaa agaaaggttt cgcgctttat atcaccatgt tcccgtcata tcagctcatt
     5761 tacggtgtgc tggcggtgat ccccattctc tttgtttggg tctactggac atggtgtatc
     5821 gtcttgcttg gcgcggaaat tactgtcact ctcggggaat accgcaaact aaaacaagca
     5881 gctgaacaag aagaagacga cgaaccatga ttgcattaat tcaacgcgta acccgtgcca
     5941 gcgtcaccgt ggagggagaa gtgacgggcg aaattggcgc gggacttttg gtgttattgg
     6001 gtgtcgaaaa ggatgacgac gaacagaaag caaaccgtct gtgcgagcgt gtgctcggct
     6061 accgcatctt tagcgatgcc gaaggcaaga tgaatctcaa cgtgcaacag gcgggcggca
     6121 gtgtactggt ggtttcccag tttaccctcg ccgcagatac cgaacggggg atgcgcccaa
     6181 gtttctccaa aggcgcatca ccggatcgcg cagaggcgtt atatgactat ttcgtcgaac
     6241 gctgccgcca gcaagagatg aacacgcaaa caggacgctt cgctgccgat atgcaggtat
     6301 cgctggtcaa tgatggcccc gtgacattct ggttgcaggt atgagccagc ttccagggtt
     6361 gtcacgggaa acaagagaga gtatcgctat gtatcacctt caggttccac aaacagaaga
     6421 agagttagag cgttactatc agtttcgctg ggaaatgttg cgtaagcccc tgcatcaacc
     6481 aaaaggttcg gaacgcgacg cgtgggatgc gatggcgcat caccagatgg tcgtcgacga
     6541 gcagggtaat ctggtggcgg taggccgatt gtatattaat gccgacaatg aagcgtccat
     6601 tcgctttatg gccgttcacc cggacgtgca ggacaaaggg ttaggaacgc tgatggcgat
     6661 gaccctggag tcggtggcgc gtcaggaagg cgttaagcgc gtgacctgta gcgcccgtga
     6721 agacgcggtg gagtttttcg ccaagctggg gtttgttaat cagggagaaa tcaccacgcc
     6781 aaccaccacg ccgattcgcc attttttgat gattaagccc gtcgccactc tggatgatat
     6841 tttgcatcgc ggcgactggt gcgcgcagct gcaacaggcg tggtacgaac atatcccgct
     6901 tagtgaaaaa atgggcgtgc gtattcaaca atataccggg caaaaattta tcaccaccat
     6961 gccggaaacc ggcaatcaga atccgcacca tacgctgttt gccgggagtt tattctcgct
     7021 ggcgacgctc accggttggg ggcttatctg gctgatgctg cgtgaacgcc acctcggcgg
     7081 aacgattatt ctggcggatg cgcatatccg ctacagcaaa ccgattagcg gtaaacccca
     7141 tgcggtagcc gaccttggtg ccttaagcgg cgatctcgac cgtctggcgc gcggacgaaa
     7201 agcacgggtg cagatgcagg tcgaaatctt tggcgacgag acgccgggtg cagtgtttga
     7261 aggcacgtat atcgttctgc ccgcgaagcc atttggcccg tatgaagagg gcgggaacga
     7321 agaagagtag cttatggaga ggggcggtgc tgcctctctc attcattccg gtcatttaca
     7381 tcagtccctg actattccgg ttatattgaa taagaacttt ataaattttt attcttcgtt
     7441 ggcaccatac gtgtcttgct acatcactgt tcaggagcat ttaaatatta tttgtagagc
     7501 tgagtggcaa ttattgcaga taatgcttaa ctcatcatct tagttcaggt ttgagcgatt
     7561 tttgatagtc aaaaccccct ctggtgccat acttttttca taaatatttt taaaatctat
     7621 agctcacaaa cattatgcaa agataatggg ctggcataca ttgtcagtat cattcattta
     7681 ctgctctgta tgttatgaat cattatgttg tatgtttgct cttaaaccgt gggaaggagg
     7741 ctataatggt tgccaatatt aatctcatta aacaagaaag ttattctgtt gttaatttag
     7801 aaaaacaact aatgagtaat aaatcaccga agcaaacgct attaaatcaa atttcacttt
     7861 gccttaaaat ggaaggtgag gagcaactgt gcgattgtgt taacgaactg aagcatggtt
     7921 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
     7981 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
     8041 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
     8101 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
     8161 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
     8221 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
     8281 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
     8341 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
     8401 gaaaaagttg aggatatgtg tgaaagaatg aaaggtggca tctctatgtt taaccacacc
     8461 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
     8521 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
     8581 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
     8641 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
     8701 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
     8761 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
     8821 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
     8881 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     8941 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     9001 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     9061 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     9121 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     9181 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     9241 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     9301 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     9361 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     9421 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     9481 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     9541 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     9601 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     9661 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     9721 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     9781 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     9841 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     9901 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     9961 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
    10021 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
    10081 atttttgagt aataaatgaa acaactgtaa cgctcttatt atttctggaa aacgcctcgc
    10141 aaaaacaaaa tgacatgcaa aaatccgctt tttttaaatg cttttccggc gtaattttcg
    10201 cacatacatt aatgagtaat atatgtaagg tggtggtaat ggcgatgaat acggtttttc
    10261 ttcatttatc agaggaggca attaaacggc tgaacaagct tcggggctgg cgtaaggttt
    10321 cacgatctgc aattttacgc gaagcggttg agcaatatct ggagcggcag caatttccgg
    10381 tgcgtaaagc aaagggaggc aggcaaaagg gggaggtagt tggtgttgat gatcagtgta
    10441 aggagcataa ggaatgaaaa aaatttttca ttcttgaata taaaaaacag atgcccttat
    10501 tctggtatta atacaaggct gttttacttg aacttataat aactgcaact gttacatcgt
    10561 atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa atgagctttc tttaaagatt
    10621 cggcaagggt acttttctct ccatagattc aaaaatgata ttggcgagat atttatgaac
    10681 tctctggcag gtatagatat gggcagaatt ttactcgatt tatcgaatga ggtgattaag
    10741 caacttgatg atctcgaggt gcagcgtaat cttcctcgtg cggacctatt aagggaagcg
    10801 gtagatcaat acctgataaa tcaatcgcaa acagcaagaa ccagtgttcc tggcatctgg
    10861 caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc tgcgcgagga atggtaatgg
    10921 gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt tgttcatgcc ggatgcggcg
    10981 tgaacgcctt atccggccta caaagtcatg caaattcaat atattgaagt ttattggtag
    11041 gcctgataag cgcagcgcat caggcaatca tacgcttgtc atcagtgaca tacccataac
    11101 cttatcgatg tgttggaggg aagaacgagg caaacagaca attgcggcat gatcttgaat
    11161 gggtaacgcg gaatacgaga gatggtgtcg tcacacccta tgagctgtaa tccgggcgac
    11221 ctgcgccacc cggaaaacgt cagagattac tctccttcac ccggaaacag gaacgggtta
    11281 atggaactac gggcgtagcc ttcttgctcc attcgcgcgt ccagtaccag agaggcgagg
    11341 tcatctgcca cggcttcaac tttcggttct ttttcctgat aaagaatctt caggtaagtg
    11401 ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag cgaccagtaa
    11461 tgcagtttgc cgctctgctc gcagttgctg cattttacgc gcactacgtg ccattcggtt
    11521 tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg tgccaatttg caccatgctg
    11581 gacaccggca tagagccaca taccgggcaa tattgacgtt gttcgccgta ttcagcgcgg
    11641 gctttgccgg ggatcagatt ggccatctgc gcccagtaga gcgacagcgc agcccagata
    11701 aacggcgctt tatcgctgct gacggacgag aaatcagagg caaacagcgc gctggccata
    11761 tcttccagct cctgagtcga tgccttctcc agattctcaa tcactgccag cgccgggccg
    11821 ctcatttcag gtttcagctc agcaattagc gccatcagca gcttttgcca gtgcttatca
    11881 cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg cgctggcttc tttaatgcgc
    11941 gcggtcagat ccatctccag cggatggtcg tacagcacca cttcctgggc gtgggcgata
    12001 agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc gcgcagacgc
    12061 tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg aatcatatcc
    12121 gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat actcattcag
    12181 ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt ttcttcgccc
    12241 aggcgttggt aacccatcct tccaccatgg cggtaatcgt gcctttcacc caaagggcgg
    12301 cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa tgcagcatta
    12361 acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc cagataatca
    12421 cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg catttctgac
    12481 cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta cgaatattct
    12541 tcgcccaaaa gatatcatcc cgattgatta ggttttgatg ccagtaacgg aaaaacatga
    12601 tgatgaacga ggcaaacata accacgccga caaacgggtg cagaattcgc gccagctgcg
    12661 gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc agcccgctca
    12721 ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc ggcgcggtgt
    12781 agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt cgtgcagatt
    12841 attctcttcc tcatccgcac ggttcggacc gacacccacg tagtggaaga tactggccgc
    12901 aaaggtagcc gcaaagccaa cagccgcgag cggtttccag atgcctttcc agaatttcac
    12961 ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct tgtcagcatg
    13021 gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc ggatcgtaca gacccgcatt
    13081 gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc gccagcgttt tcatcgactc
    13141 tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggctg gttcttgccc
    13201 aaccaccacg cggtcaacgc acagcgtaca tttgtagacg cggttgtctt ccgggttgag
    13261 gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccaatgc actgctcgga
    13321 ctggaagtcg acgataccgt tggcatactg aataattgca ccttccgccg ggcacgcttt
    13381 caggcagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc attccagttt
    13441 gtcgttctgc tccacttccg agaagcgcat taccgtccac gatttggcgc ttaaatcatt
    13501 ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt tccactctga
    13561 acacgccacc tgacaggctt tacagccgat acaggtggta acgtcgatga gtttcgccac
    13621 ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg
    13681 aatgatatct tgagattgat aagccatatg tcgtctccgt tacacctttt ccacattcac
    13741 aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccaacgaatg gcgttaaagt
    13801 attggcaata aagccttttt tagctacgcc ttcatagccc cagtgaatcg gaataccgat
    13861 ggtatcgata tctttgccgt tcgctttcag cgtgcgaata cgtttggtca ccaccgcttt
    13921 ggctttgata tagccacggt tggaggagac tttcacggta tcgccctggg caatgccaag
    13981 tttattcgcc agcgactccc cgatttccac aaactgctct ggctgcaaaa tcgcgttcaa
    14041 cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga cgataggtgg ttccgacata
    14101 cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagatac gtgcagccgg
    14161 gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa acggctcgta
    14221 gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgcc ccatgccttc
    14281 ctgctgcatg ataaacggcc cgacgccgct acccggaggc gctgcgctgt agtccggaat
    14341 atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg gatcccacgg
    14401 gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa gcggccatgc
    14461 ccatgcccag cccagcgtgt taccgaggcc agacggatcg gcgttatcac gacgcgccat
    14521 ctggttgcct tccggcgtcc agctaccggc gaaaatccag cagccacagg aggttgtacc
    14581 gtcatcgcgc aattgggcga acgagctaag ttgttggcct ttcttgacga taaccgcacc
    14641 ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg ctacttcttc
    14701 cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg ttcagcacct gatccgggtt
    14761 cgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg agaggatctc
    14821 accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc cagtgccact gcaaccagcg
    14881 acctgagtta acgattgaac cgttctcttc tgcgaagcag gtcgatggca gacggaacac
    14941 ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct gccagaagtt
    15001 agaggtttca gtgttcagtg ggtcgatggt gacgaggaac ttcagtttcg acagacaacc
    15061 gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc agatatagcc
    15121 attgaccttg ccctctttca tcatctcgaa gtattgcagg acgtcgtagc ctttatccca
    15181 cttcggcaac cagtcaaagc cccagctatt ttccgccgtc gctttatcac caaagaaggc
    15241 cttcatcata gagacgaaga atttcgggta gttgccccag tagtttacct ggccttccag
    15301 cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga tcggtctgct tctcgcttgg
    15361 cagcgtcatg taacctggca gactctgcga cagtagcccc aggtccgtca gcccctgaat
    15421 attggagtga ccgcgcaggg cgttaacgcc gccgccggcc atccccatat tgccgagcag
    15481 cagctggatc atcgccatcg tacgaatgtt ttgcgcacca acggagtgtt gcgtccagcc
    15541 gagggcatac aggaacgagg cagttttatc gtgagcactg gtttctgcga tgtattcgca
    15601 gactttcagg aacgcgtctt ttggcgtacc acagatgttt tcaaccacat ctggcgtgta
    15661 gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc ggatgttgca gcgtggtatc
    15721 gcgtttggcg aagccgtttt cgtccagttc ataagtccag gaggatttat cgtacttgcg
    15781 tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat
    15841 caggctggcg ttggtatagg tttcggtgta ttcgtggttg aatttttcat tgttcagcag
    15901 gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacgaatag gggcatagta
    15961 gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc acaatcagct tcgcgccgtt
    16021 gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag cggcgttacc
    16081 gcccatcacc acgacgaggt tggcgttctt gatgtcgacc cagtggttgg tcatcgcacc
    16141 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac gcgcctggtt
    16201 gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt
    16261 actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag tcacgccttc
    16321 ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc
    16381 aaacgcctct tcccaactga tttgctgcca tttatcagaa cctggcgcac ggtattccgg
    16441 aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt tcggacaaag
    16501 tgcaccgcgg ttgaccgggt gatccggatc gccttcgata tggaagatag atgctttggc
    16561 gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg aacaataggt
    16621 gcaggtatta cgggtttcgc gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc
    16681 tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc cagcgcagat
    16741 cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac attgtcacgt
    16801 cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg
    16861 ggccgcaaag gtccagaatt ggctcaattt ccctccatct aggaagggtt tgtaacagaa
    16921 taccataatg ttggtgtgtg tgttcttatc tggttaagag aaagtgaaaa aaacacagcg
    16981 aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt caaattgagt tatggcggcg
    17041 tgatgattta caacacccac ggttagatga ggtcgcggaa gaagttcccg ttgcgctggt
    17101 ctacaacggt atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg agtactttgc
    17161 gctcggtttt tcgctttccg aggggataat agaaagtccg cgcgatatct tcggcatgga
    17221 tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag ctttccagcc gccgctttat
    17281 ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg tatgcggcgt
    17341 ggagcaactt aatgacatcg gaaaaccggt gcagccgcta ccgttcaccc agacgtttga
    17401 tctcaacaaa ctggatgatg cattacgtca tctcaacgat tttcagccag tggggcaact
    17461 gactggttgt actcacgccg ctgcctggat gttgccatct ggcgaactgg tcggcgggca
    17521 tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt cacaagaagg
    17581 ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt gccagttatg aaatggtgca
    17641 aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga ccacgcttgc
    17701 tgtagaagtg gctgagcgct gtaatctgac actggtaggt ttttgtaaac cgggtcgggc
    17761 aacggtttat acccatccgc agcgtttgag caattaatta aaataagtcc tgcgatatat
    17821 attaaattgc aggacttatt catttcgtga attttattat tttatttata aataaa
//
LOCUS       DADKXM010000077        17614 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.078, whole genome shotgun sequence.
ACCESSION   DADKXM010000077 DADKXM010000000
VERSION     DADKXM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.078"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JQR05_002536"
     CDS             <1..848
                     /locus_tag="JQR05_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY5324684.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASADLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVAWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWMNALEQEWLR
                     RYGTH"
     gene            821..2357
                     /locus_tag="JQR05_002537"
                     /pseudo
     CDS             821..2357
                     /locus_tag="JQR05_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2357..3010
                     /locus_tag="JQR05_002538"
     CDS             2357..3010
                     /locus_tag="JQR05_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5324685.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3077..4384
                     /locus_tag="JQR05_002539"
     CDS             3077..4384
                     /locus_tag="JQR05_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY5324686.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVENEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(4393..5013)
                     /locus_tag="JQR05_002540"
     CDS             complement(4393..5013)
                     /locus_tag="JQR05_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY5324687.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            5100..5507
                     /gene="nudG"
                     /locus_tag="JQR05_002541"
     CDS             5100..5507
                     /gene="nudG"
                     /locus_tag="JQR05_002541"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY5324688.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5473..5745)
                     /locus_tag="JQR05_002542"
     CDS             complement(5473..5745)
                     /locus_tag="JQR05_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY5324689.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5981..7324
                     /gene="gdhA"
                     /locus_tag="JQR05_002543"
     CDS             5981..7324
                     /gene="gdhA"
                     /locus_tag="JQR05_002543"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY5324690.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(7441..8481)
                     /locus_tag="JQR05_002544"
     CDS             complement(7441..8481)
                     /locus_tag="JQR05_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY5324691.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8609..10570)
                     /locus_tag="JQR05_002545"
     CDS             complement(8609..10570)
                     /locus_tag="JQR05_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY5324692.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JQR05_002546"
     CDS             complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JQR05_002546"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY5324693.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11735..12286)
                     /locus_tag="JQR05_002547"
     CDS             complement(11735..12286)
                     /locus_tag="JQR05_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY5324694.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12447..14303
                     /gene="sppA"
                     /locus_tag="JQR05_002548"
     CDS             12447..14303
                     /gene="sppA"
                     /locus_tag="JQR05_002548"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY5324695.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSPHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14470..15486
                     /gene="ansA"
                     /locus_tag="JQR05_002549"
     CDS             14470..15486
                     /gene="ansA"
                     /locus_tag="JQR05_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY5324696.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15497..16138
                     /gene="pncA"
                     /locus_tag="JQR05_002550"
     CDS             15497..16138
                     /gene="pncA"
                     /locus_tag="JQR05_002550"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY5324697.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16231..17590)
                     /locus_tag="JQR05_002551"
                     /pseudo
     CDS             complement(16231..17590)
                     /locus_tag="JQR05_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4262 a         4639 c         4477 g         4236 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcgccga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gcctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg atgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggcatcg gtctgggcct cacccttgca gtgaaagaaa gcgcatttct
     1261 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
     1321 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc
     1381 gccttcgctg gcaatggcga tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt
     1441 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
     1501 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
     1561 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
     1621 tccccgcgta gatggcattc gcaatgcctg ccacaccttt attaccaggc actacgctgg
     1681 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
     1741 agtcgacgat caatagtgaa gcactcatca acagtctgac aatggggctg gtagcaacat
     1801 tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg ggcacagcgt cgccagttgt
     1861 ggctatggtt gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg
     1921 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
     1981 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
     2041 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtgaaatgcc
     2101 cactcatgtt gcgccctgtg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
     2161 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg
     2221 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgt
     2281 tgttaccgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
     2341 gacaaggaat ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
     2401 cttgctgaca aacgttaact ttacggttga taaaggtgac atcgtcacgt taatggggcc
     2461 gtctggctgt ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ccggacagtt
     2521 ttcttgtaca ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca
     2581 gcgtcagatt ggcattcttt ttcaggatgc actgttattt gaccagttca gcgtcgggca
     2641 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
     2701 tgatgcactt gagcgttcag gccttgacgg agctttccat caggatcctg ccactttgtc
     2761 tggcggtcag cgagcgcgcg ttgctctact acgcgccctt ctcgcccagc caaaagcgtt
     2821 actcctggat gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg
     2881 ggtgttcagc gaagttcgcg ccctggcgat ccccgtcgtt caggtaacgc acgatctcca
     2941 ggatgttcct gctgatagtt ctgttctgga tatggcgcag tggtcagaaa attacaacaa
     3001 actgcgataa cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac
     3061 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
     3121 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccaact
     3181 tcagcaacaa aatggcaaag cgatagatac tcgccccagc gcgttttata acggctggcc
     3241 acaaacctta aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgctagctg
     3301 gctcgacaaa atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc
     3361 cgatgccccg gtggcgctgt acggtaatgg caaagatgtc gacgccgtca aaacgcgact
     3421 gcaaaaagca ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg
     3481 tctacaaaaa ctgccgcact ttgagcagct ggtttatccg caatggctgc atgacctgca
     3541 acaaggtaaa gaggttacgg cgaaacctgt cggtgactgg aaagtcattg aagcggcctg
     3601 gggcgctcct aagctttacc ttatcagcca tattcctggc gctgactaca tcgataccaa
     3661 cgaagtggaa aacgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct
     3721 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
     3781 tggagcgcgt gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga
     3841 tggcggctgg caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa
     3901 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga
     3961 tatggaacag gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
     4021 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
     4081 agccggagca cgttggggac acgctggtag cgactcgacg catatggaag atttccataa
     4141 cccggatggc accatgcgta gcgccgatga tattaccgct atgtggaaag catggaatat
     4201 caaaccagat cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt
     4261 tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga
     4321 atggagcagc gatccaaaaa atccggttgc aaccggtgaa cgcggcccgg gcagtagcaa
     4381 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
     4441 cgtaaaggtc gtcatccagc acaacgcacc gaagatccac gcaaaccacg caaaccatgc
     4501 agggaataaa catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaatccccc
     4561 cagatagtaa aacgacttat gtgcataacc ggggttatca atctgatgtt ttgctgccag
     4621 tgcagcaaag gcgagaaaac tgctgcccgt gccgataaac gcaaacaaca accagccgcc
     4681 tgccagcgca ttttgctccg gtgcagcaag aataaagcca aacggcacca gcgcgtaaaa
     4741 gagaaaatcg agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
     4801 cagcgcaccg tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag
     4861 ataccagccc agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt
     4921 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
     4981 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
     5041 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
     5101 tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc
     5161 aacgccccgc ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
     5221 tggatgaaag ccagcagcag gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag
     5281 caactgtggg tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc
     5341 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
     5401 tggtctggtg ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat
     5461 tattagaggc gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt
     5521 gtcatcacgc tggcattgca gcagtattcc ttcagcttta attaccgccc cttcagaata
     5581 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
     5641 gaacacttct ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc
     5701 aattaatgga aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac
     5761 gttattgtct ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat
     5821 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
     5881 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
     5941 cataagcaca atcgtattaa tatataaggt ttttatatct atggatcaga catattctct
     6001 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
     6061 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
     6121 gatgtcatta ttggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
     6181 ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
     6241 catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa
     6301 attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg
     6361 taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtaaagtga tgcgtttttg
     6421 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg
     6481 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
     6541 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg
     6601 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg
     6661 tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
     6721 tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac
     6781 tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc
     6841 cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg
     6901 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
     6961 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc
     7021 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
     7081 accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa
     7141 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
     7201 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
     7261 gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat
     7321 ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacagatggg cacaattcat
     7381 tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt
     7441 ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt
     7501 cttactgtag tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc
     7561 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt
     7621 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
     7681 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat
     7741 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
     7801 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
     7861 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
     7921 aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt gattctctaa
     7981 agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat gatattggaa
     8041 ggtatctttg ccaggttcag gctttcgcgg ccagaactct agcgtttcag accattgctt
     8101 atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg
     8161 ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg
     8221 aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg taatcaggcc
     8281 tctttcattc aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca
     8341 tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa tcacccgttc
     8401 aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt
     8461 ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag gcgataattt
     8521 tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg
     8581 actcgaatag cacgaaagat tcgctcgctt acgctatcgc cccgcttccg gcttcatctg
     8641 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
     8701 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
     8761 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
     8821 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
     8881 tttctggcag cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt
     8941 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
     9001 tccccagacc atcggtcgca cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg
     9061 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
     9121 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
     9181 atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
     9241 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
     9301 cacacttgcg gaacaccgca tccgggcaga actgcatcag gtattgacgg gcaatcaggt
     9361 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga
     9421 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
     9481 ccactggctg cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc
     9541 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     9601 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc
     9661 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt
     9721 cccgtttatc gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat
     9781 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     9841 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     9901 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     9961 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
    10021 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
    10081 ccagcgcaga aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc
    10141 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
    10201 ccggtgccag ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg
    10261 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
    10321 gtttggtcac ggagggtcgt ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
    10381 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    10441 gaccgataca ccaagtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    10501 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    10561 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    10621 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct
    10681 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    10741 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg
    10801 ccgccaggta ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct
    10861 tcatagtcga cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc
    10921 agaccaaagc ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
    10981 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac
    11041 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
    11101 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    11161 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    11221 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
    11281 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
    11341 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    11401 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    11461 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    11521 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
    11581 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    11641 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
    11701 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
    11761 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
    11821 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    11881 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
    11941 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttctttgc
    12001 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
    12061 tagctttatc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
    12121 cgcacccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
    12181 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    12241 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    12301 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
    12361 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
    12421 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    12481 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    12541 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    12601 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaaccag
    12661 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    12721 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    12781 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    12841 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    12901 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    12961 caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
    13021 tgctggataa gctgaaagtt tccccccatg tgttccgcgt gggtacgtat aaatctgccg
    13081 ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga
    13141 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
    13201 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    13261 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    13321 aaaaagctct gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
    13381 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
    13441 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    13501 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
    13561 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    13621 cccgggcagc gggtaagtct gtggttgtat cgatgggcgg catggcggca tctggtggtt
    13681 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
    13741 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    13801 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    13861 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
    13921 ttgctgatgc gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct
    13981 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    14041 cggttgccaa agcagcagag ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg
    14101 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    14161 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
    14221 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    14281 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
    14341 tattttttac ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
    14401 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
    14461 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
    14521 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
    14581 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg
    14641 atggattctt ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg
    14701 cactatgacg actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc
    14761 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
    14821 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
    14881 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
    14941 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
    15001 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac
    15061 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
    15121 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
    15181 attctgcgct cctatggcgt gggtaatgcg ccacaaaaca aagccttcct gcaggaatta
    15241 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
    15301 gtgaatatga gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
    15361 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
    15421 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
    15481 gattaaggag actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt
    15541 ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt acggtggagg ttgctaaccg
    15601 ccttattgac tggtgccagt cgcgcggaga agcggttatc gccagccagg actggcatcc
    15661 ggcgaatcac ggcagctttg ccagccagca cggcgtagag ccttatacgc caggccaact
    15721 cgacggcctg cggcaaactt tctggcctga acactgtgtg cagaacagtg aaggcgcaca
    15781 attacatccg ttactgaatc aaaaagagat cgcagcggtg ttccataaag gcgaaaatcc
    15841 tttagttgac agttacagtg ccttttttga taacggacgt cggcagaaaa ccgcgcttga
    15901 tgactggttg cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta
    15961 ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac
    16021 cgatggctgt cgtggcgtga atatccagcc ccaggacagt gcacacgcgt ttatggagat
    16081 gtcagcagct ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaatt
    16141 ttacgctggc ctacaattct gtactggcat tgtaggccaa ataaaacacg tcagtggtac
    16201 atctggcaat tgatgccatc aacgaaagat ttaattcacc tcagaaatct cttccagcga
    16261 caccttccgc gtttcgatgc caaaaatgga gagaaccagc gcacagagca ctaacatgac
    16321 accaagcacc ataaacaccg tgatcgaccc ataatgtgtt aataatgccg caacgccata
    16381 gggcgtgaaa actgcgacga tccgtccgac ggcattaacg aaacccgaac cgcgcaggcg
    16441 taaatgcgtt ggccaaagct ccgggatata aaccgccgac gcgaagcaaa catacatgta
    16501 taaaaagaag atcatcacca gaccatagat taaaatcgcc cactctgtag tctgacttga
    16561 atagatatag cctaacacgg caataataat aagtaaggcg gagccaaata atcgacgagg
    16621 aaaatgatca ataattaatg ccgcaataaa tattcctacc ggagcaccaa tcataataac
    16681 agcggtcatt aatattgatt tatcgacatc aatgccggag ttaacaaata tggtcggtat
    16741 ccatacggta atggtataaa gcgaaatgtt cattgcaatt aaaacagtta tcgcaactaa
    16801 tgtacgtcgt aacatttccc ctttaaacag gagccagaag gtacccttga ttaccttaac
    16861 cttgctgttg ctctggttca aagtaagctg gggtaaacga atactcttct ccctttcaat
    16921 ttgctgctct acttcacgaa gttggctttc tgcaccggcg atttgccctt tccctgccag
    16981 ccatcgtggc gactcgataa agtatttacc tgagagaaac caggctaaca gtatgccaat
    17041 accacccaga agaaacatta ttcgccaact aaaaaaaagc gataaccacc acgcctatcg
    17101 ccgcagacag catgggcgac cagttaccaa caaatgagag ccgcgcggac cattttccac
    17161 gcaccttcgc ggggataaac tccgtaaatg aggcatagcc aaccataatc agcgccccca
    17221 ttcctgttcc catcaggaag cgaaagaaaa tgagccagta catatcaggg acaaaagcgg
    17281 cccctgttgc agcaataccg acgatgagaa gatttatgcg aaacgccctg cgccgcccaa
    17341 agtagtcacc aataaaccca ccagtaagtg agccgatgaa ataaccaaac attaatgccg
    17401 aggtaaaggc ggcattgagg aaattatttg accatccatt gcttaccagc tttgctaaga
    17461 cgacattacc tgagtaactc aaaaaccccg ttaataacag actaaagctt attataccaa
    17521 atatacgata atgaaaccgg gccaaaggca agcggtccag tcttgcgcca atttgatcat
    17581 attgttccat tgggtgcctc ggatttgcta aaaa
//
LOCUS       DADKXM010000078        17515 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.079, whole genome shotgun sequence.
ACCESSION   DADKXM010000078 DADKXM010000000
VERSION     DADKXM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.079"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1247
                     /locus_tag="JQR05_002552"
     CDS             <1..1247
                     /locus_tag="JQR05_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY5324698.1"
                     /translation="MGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDADYWFDGA
                     NYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPVRAEEPFL
                     MVVSYDEPHHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQAMPSPVGDD
                     GLYHHPLYFACNDFVDDQIGRVINALTAEQRENTWVIYTSDHGEMMGAHKLISKGAAM
                     YDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPR
                     GVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDD
                     IRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPRPQDGYSPV
                     VRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(1294..1707)
                     /locus_tag="JQR05_002553"
     CDS             complement(1294..1707)
                     /locus_tag="JQR05_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324699.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            1816..2163
                     /locus_tag="JQR05_002554"
     CDS             1816..2163
                     /locus_tag="JQR05_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY5324700.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            2153..2515
                     /locus_tag="JQR05_002555"
     CDS             2153..2515
                     /locus_tag="JQR05_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY5324701.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            2512..3009
                     /locus_tag="JQR05_002556"
     CDS             2512..3009
                     /locus_tag="JQR05_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY5324702.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="JQR05_002557"
     CDS             complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="JQR05_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY5324703.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="JQR05_002558"
     CDS             complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="JQR05_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY5324704.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            5135..5233
                     /gene="ivbL"
                     /locus_tag="JQR05_002559"
     CDS             5135..5233
                     /gene="ivbL"
                     /locus_tag="JQR05_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY5324705.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            5339..7027
                     /gene="ilvB"
                     /locus_tag="JQR05_002560"
     CDS             5339..7027
                     /gene="ilvB"
                     /locus_tag="JQR05_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY5324706.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVGSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            7031..7321
                     /locus_tag="JQR05_002561"
     CDS             7031..7321
                     /locus_tag="JQR05_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY5324707.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            7395..7985
                     /gene="uhpA"
                     /locus_tag="JQR05_002562"
     CDS             7395..7985
                     /gene="uhpA"
                     /locus_tag="JQR05_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY5324708.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            7985..9487
                     /gene="uhpB"
                     /locus_tag="JQR05_002563"
     CDS             7985..9487
                     /gene="uhpB"
                     /locus_tag="JQR05_002563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY5324709.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            9497..10816
                     /gene="uhpC"
                     /locus_tag="JQR05_002564"
     CDS             9497..10816
                     /gene="uhpC"
                     /locus_tag="JQR05_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY5324710.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            10954..12345
                     /gene="uhpT"
                     /locus_tag="JQR05_002565"
     CDS             10954..12345
                     /gene="uhpT"
                     /locus_tag="JQR05_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY5324711.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(12391..14143)
                     /gene="adeD"
                     /locus_tag="JQR05_002566"
                     /pseudo
     CDS             complement(12391..14143)
                     /gene="adeD"
                     /locus_tag="JQR05_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            14318..15652
                     /locus_tag="JQR05_002567"
     CDS             14318..15652
                     /locus_tag="JQR05_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5324712.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            15705..16157
                     /locus_tag="JQR05_002568"
     CDS             15705..16157
                     /locus_tag="JQR05_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY5324713.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            16368..>17515
                     /gene="nepI"
                     /locus_tag="JQR05_002569"
     CDS             16368..>17515
                     /gene="nepI"
                     /locus_tag="JQR05_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY5324714.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVVFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNIFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLT"
BASE COUNT         3861 a         4435 c         4838 g         4381 t
ORIGIN      
        1 ctatggggcg ctactttaag gatgccggct atcacacctg ttacatcggc aaatggcatc
       61 tcgacggtca tgactatttc ggcactggcg agtgtccgcc ggagtgggat gctgattact
      121 ggtttgatgg ggcgaactat cttagcgaac tgacggaaaa agagatcagc ctgtggcgca
      181 atggcctaaa cagcgtcgaa gatttacagg cgaaccatat tgacgaaacc ttcacctggg
      241 cgcaccgcat cagcaatcga gcggtagatt ttctgcaaca gcccgtgcgc gccgaggaac
      301 ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc gttcacctgt ccggtggagt
      361 atttagagaa atacgctgat ttttactacg aacttggcga gaaatcacag gatgacctgg
      421 cgaacaaacc ggaacatcac cgcttatggg cgcaggcgat gccatcgcca gtcggtgatg
      481 acgggcttta tcaccatccg ctctattttg cctgcaatga ctttgttgat gaccaaatcg
      541 gacgtgtcat caatgcctta acggcagagc aacgtgaaaa tacgtgggtc atttatacct
      601 ccgatcacgg tgaaatgatg ggcgcacata agctgatcag taaaggagcg gcgatgtatg
      661 acgacatcac ccgtattccg ctgatcatcc gttcgccgca aggggagcgg cgacaggtcg
      721 atacgccagt cagtcatatt gatttactgc cgacaatgat ggcgctggca gatattgaaa
      781 aaccagagat tctgccgggg gaaaatatcc ttgccgtgaa agagccacgc ggcgtgatgg
      841 tggaatttaa ccgctacgag attgagcatg atagctttgg cggttttatt ccggtgcgtt
      901 gctgggtgac ggatgacttt aaactggtgc tcaacctctt caccagtgat gaactttacg
      961 atcgccgtaa tgacccaaat gaaatgcata acctgatcga tgatatccgt tttgcagacg
     1021 ttcgcagcaa aatgcatgac gccttattgg attacatgga caaaattcgc gatccgttcc
     1081 gcagttacca atggagtctg cgtccgtggc gtaaagatgc acgaccgcgc tggatggggg
     1141 cgtttcgccc acgcccacaa gatggctatt cgccagtggt acgcgactat gacaccggcc
     1201 taccgacgca aggagtgaag gtggaggaga aaaaacagaa attctgacga taatgcccgc
     1261 tgatgatcac ccggcgggca ttattcaggc tgcttaaacc agcgaaccgc cggtcaggaa
     1321 ggaaaaaatc aggaatatca gcaaaatgct gcacacgatg ccgataccaa aaaccagcgc
     1381 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
     1441 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgatatcca tgtcctcttt
     1501 tttctccaca gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
     1561 agccgttttg tccgccacga tgctttcaag cgttggcggt ggtgcggaaa aagggccgaa
     1621 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
     1681 gattttattt tgcgctatga tacccatctg acctccttgt agcggatggc aaaagcatat
     1741 caggaaacgg gcggacgaaa ttcttaaaaa aaaccgctaa agatagagcg ttagtgaaat
     1801 tgcaaaggag caacaatgaa aatttcccgc cttggagaag cgcccgatta ccgcttctcg
     1861 ctggctaatg agcgtacttt tctggcgtgg atccgtaccg cactaggatt tctggcggcg
     1921 ggggttgggc ttgatcaact tgcgccagat ttcgccacgc ccgtcattcg tgaactgctg
     1981 gcgttgttgt tgtgtctgtt ttccggcggg ttagcaatgt atggctacct gcgctggtta
     2041 cgcaatgaaa aagcgatgcg tctgaaagag gacctgccct acaccaacag cttattaatc
     2101 atcagcttaa tattgatggt tgtggcggtg atcgttatgg gactggtgtt gtatgccgga
     2161 tagccgtaaa gccagacgca ttgccgaccc agggctgcag ccggagcgca catcactggc
     2221 gtggtttcgt accatgctgg gctacggcgc gttgatggcg ttggctatca aacacaactg
     2281 gcaccaggcg ggcatgttat tctggatttc gattggcatc ctcgccatcg tggcgctgat
     2341 cctctggcac tacacccgta atcgcaattt aatggatgtc acgaatagcg atttttccca
     2401 atttcacgta gtccgtgaca aatttttgat ctccctcgcg gtgttatctc tcgcaatact
     2461 gtttgctgta acgcatatac atcaacttat cgtatttatt gagagagtcg catgacagga
     2521 agtcaggtca ttgatgctga agaggacagg cataagctgg ttgtagagta caaggatgct
     2581 ctgcaacctg ccgattttta tcacaatttc aaacagcggg gcatccgctc cgtacagctc
     2641 attccccatc ttgaatttga tgaccggggc gatctgacgg ccgcttcggt gacagcagag
     2701 ctgtggggga aatttttaat tgccctgttt gagtgttggg tgcgggcaga tatcagtcgt
     2761 atctcgattg agctttttga cgccacctta caaaaatggt gtggcagcga aaacccgcaa
     2821 ccgcgccgtg actgccaggc gtgcgactgg catcgcctgt gcccgcacgc tcggcaggag
     2881 acgccagaca gcgtgctttg tgcgggctat caggcgtttt attcttactc cgcaccgcat
     2941 atgcgggtga tgcgtgatct gataaagcag catcgttctc ccatggagct gatgacgatg
     3001 ctgcggtgac gtgcgcttaa acgggctgcc cctgatgcga catccgcgtc gccagcggca
     3061 gccagcacag cacgatcaac aatcccatta aggtcatcaa caaccccagg ctgccctgac
     3121 cggtttgcgg caacatcgca gagagcgacg ccagcacgcc ggaaccaatg ttttgcagac
     3181 cgccgaccag cgcaccagcc gtgcccgcca ggaaggggaa cggctccatc gcgccgctgg
     3241 tcgccagcgg aaacagcatc ccggcaccga aaaagaacag cgcggcggga acgagcagcg
     3301 tccagacatt catcacgcca aaccagtcgg ggatccacat cagcaagcca gccagcaggc
     3361 agcagataac cgactgccac atcaacgttg agaagcgttt attaggacgc ccggcaaacc
     3421 atgccccaaa aaatgccgcc ggaatcggca gaataaacaa aatactgacc gtcatactgc
     3481 tcagccctaa caccgcgccc atcagcacgc cggagcaggc ttcaaaggca gcaatcccgg
     3541 ccagaccgcc aatcagcatc agcaaataac agttaaaacc gctgttaccg aaaagcgttt
     3601 tataactggt aagcaggcgc gtgcgcggtg catcgaccgg acgcgtttcc ggcatccagc
     3661 gggccatact gaaggtcaca ccagcacaaa gaaccaacaa aaagagataa caggcgcgcc
     3721 agttccacat cgtatccagc agaccgccga ttagcggtgc gagcaacgga ctgacgagaa
     3781 tccccatgtt taacaggctg ttagcatggc gcaactgtgt ccgttcatat aaatcacgcg
     3841 gcaaagtacg cgccattacg ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg
     3901 caatcaatac cgtcaaactg gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg
     3961 acattccgac gaggatcact ggtcggcgac cgacacggtc ggatatcggg ccataaaaca
     4021 gctgtgagac accgtaagtc agcagataag cgcccattac gctctgcacc gccccttcac
     4081 ggacgttgag atcgcgcgcc atatcggcaa tagctggaat ataaatggtt tgcgccatct
     4141 gaccgacggc cacgagtaat accaacatca ataacaaatt gacgtttctt tgccttttca
     4201 ttatcacgga tgcttttata aaaataatta gaataagtca ctacgcattc ccataaatgc
     4261 gggagaggaa tctatcacat tgatgaatgt tagccagata tacgccagga atggggaatt
     4321 gtttagcggc agaatatgta aacaaaagcg gcaataaatg ttgccgggag ggatggtagg
     4381 gcggataaga tgcgtcagca tcgcatccga ctccaacccg cacgctaaat ataaaagggg
     4441 agcggttacc cgctcccctt tggtgcgact tgaatctgaa ttacttcagg tatttcagaa
     4501 cagcatcaag cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca
     4561 ccaggctcat acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct
     4621 gcctgttgcc agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat
     4681 aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact
     4741 tagtgcgccg ggtaacgatg cggcccccgc ataacacatt gcgtacagtg atattataga
     4801 agtttactgt ataaataaac agtaatattt ggacaaaacg caaactgtga tcgatactca
     4861 actatgcact aaatacgtca aaattcgtgc cgaaattgcg cgttctgcgc ggaacacgta
     4921 tactttcagt gttgacataa tacagtgtgc tttgcggtta ccagccgcag gcggctgacg
     4981 aaacctcgct ccggcggggt tttttgttat ctgcaattca gtacaaaacg tgatcaaccc
     5041 ctcaattttc ccttgctgaa aaattttcca ttgtctcccc tgtaaagctg tgcttgtata
     5101 aatattgtta aacacaaaac caacaaggtc cccaatgact acttccatgt tcaacgcaaa
     5161 actactacca actgcgccat ccgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg
     5221 caatgcgccg tagggactgg aacaacacac gattccaaaa ccccgccggc gcaaaccggg
     5281 cggggttttt cgtttaagca cctcccggaa agtcggccca gaagaaaagg actggagcat
     5341 ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttca
     5401 tttcctggaa cagcagggca ttaagattgt gacgggcatt ccgggcggtt ctatcctgcc
     5461 tgtttacgac gccttaagcc aaagcacgca aatccgccat attctggctc gccatgaaca
     5521 gggcgcgggg tttatcgctc agggaatggc gcgcaccgat ggtaaaccgg cggtctgtat
     5581 ggcctgtagc gggccgggtg cgactaacct ggtgaccgcc attgctgatg cgcggctgga
     5641 ctccatcccg ctgatttgca tcactggtca ggttcccgcc tcgatgatcg gcaccgacgc
     5701 cttccaggaa gtggacacct acggcatctc tatccccatc accaaacaca actatctggt
     5761 cagacatatc gaagagctcc cacaggtcat gagcgatgcc ttccgcattg cgcaatcagg
     5821 ccgccctggc ccggtgtgga tagacattcc taaggatgta caaacggcgg tttttgagat
     5881 tgaagctcag cccgcggtgg cagaaaaagc cgctgcaccc gcctttagcg aagaaagcat
     5941 tcgtgacgca gctacaatga ttaacgctgc caaacgcccg gtgctttatc tgggtggtgg
     6001 tgtgatcaat gcgcctgcgc gggtgcgtga actggcggag aaagcgcaac tgcctaccac
     6061 catgacttta atggcgctgg gcatgctgcc aaaagcgcat ccgttgtcgc tgggtatgct
     6121 ggggatgcac ggcgtgggca gtaccaacta tattttgcag gaggcggatt tgttgattgt
     6181 gctcggtgcg cgttttgatg accgggcgat tggcaaaacc gagcagttct gtccgaatgc
     6241 caaaattatt catgtcgata tcgatcgcgc agagctgggt aaaatcaagc agccgcatgt
     6301 ggcgattcag gcggatgttg atgacgtgct ggcgcaattg atcccgcagg tggaagcgca
     6361 accgcgtgca gagtggcacc agttggtagc ggatttgcag cgcgaattcc cgtgtccaat
     6421 cccgaaagcg tgcgatccat taagccatta cggcctgatc aacgccgttg ccgcctgtgt
     6481 cgatgacaat gcgattatca ccaccgatgt gggtcagcat cagatgtgga ccgcgcaagc
     6541 ttatccgctc aatcgcccac gccagtggct gacctccggt gggctgggca cgatgggttt
     6601 tggcctgcct gcggcgattg gcgcggcgct ggcgaacccg gatcgcaaag tgttgtgttt
     6661 ctccggcgac ggcagcctga tgatgaatat tcaggagatg gcgaccgcca gtgaaaatca
     6721 gctggatgtc aaaatcattc tgatgaacaa cgaagcgctg gggctggtgc atcagcaaca
     6781 gagtctgttc tacgagcaag gcgtttttgc cgccacctat ccgggcaaaa tcaactttat
     6841 gcagattgcc gccggattcg gcctcgaaac ctgtgatttg aataacgaag ccgatccgca
     6901 ggctgcattg caggaaatca tcaatcgccc tggcccggcg ctgatccatg tgcgcattga
     6961 tgccgaagaa aaagtttacc cgatggtgcc gccaggtgcg gcgaatactg aaatggtggg
     7021 ggaataagcc atgcaaaaca caactcataa caacgtaatt ctggagctca ccgttcgcaa
     7081 ccatccgggc gtaatgaccc acgtttgtgg cctttttgcc cgccgcgctt ttaacgttga
     7141 aggcattctt tgtctgccga ttcaggacag cgacaaaagc catatctggc tactggtcaa
     7201 tgacgaccag cgtctggagc agatgataag ccaaatcgat aagctggaag atgtcgtgaa
     7261 agtgcagcgt aatcagtccg atccgacgat gtttaacaag atcgcggtgt tttttcagta
     7321 accgctcaag gcttgaacaa catcgcgctt atcgttaagg taagcgcgtt ttttttaccc
     7381 gccaggacaa gaccatgatc accgttgccc ttatagacga tcacctcatc gtccgctccg
     7441 gctttgcgca gctgctgggg ctggaacctg atttgcaggt agttgccgag tttggttcgg
     7501 ggcgcgaggc gctggcgggg ctgccggggc gcggtgtgca ggtgtgtatt tgcgatatct
     7561 ccatgcccga tatctccggt ctggagctgc taagccagct gccgaaaggt atggcgacga
     7621 ttatgctctc cgttcacgac agtcctgcgc tggttgagca ggcgcttaac gcgggggcac
     7681 gcggctttct ttccaaacgc tgtagcccgg atgaactcat tgctgcggtg catacggttg
     7741 ccacgggcgg ctgttatctg acgccggata ttgccattaa actggcatcc ggtcgtcagg
     7801 acccgctgac caaacgtgaa cgccaggtgg cggaaaaact ggcgcaagga atggcggtga
     7861 aagagattgc cgccgaactg ggcttgtcac cgaaaacggt acacgtccat cgcgccaatc
     7921 tgatggaaaa actgggcgtc agtaacgacg tagagctggc gcgccgcatg tttgatggct
     7981 ggtgatgaag acgttgttct cccgcttaat taccgttatt gcctgctttt ttatcttctc
     8041 tgccgcatgg ttttgcctat ggagtatcag cctgcatctg gttgagcgcc ctgatatggc
     8101 ggtgctgtta tttccgtttg gtctgcgtct ggggctaatg ctgcaatgcc cgcgcggata
     8161 ctggcccgta ttgctgggtg cggagtggct gctgatttac tggctaacgc aggcggtcgg
     8221 tttaacccat tttccgttat tgatgatcgg tagtttactg acgttactgc ccgtagcgct
     8281 gatctcgcgc tatcgccatc agcgtgactg gcgcaccttg ctgttacagg gtgcggcgtt
     8341 aacggcggcg gcgttgttgc agtcgctgcc ctggctttgg cacggcaaag agtcgtggaa
     8401 tgcgctgttg ctgactttaa ctggcggtct gacgctggcc ccgatatgtc tggtgttctg
     8461 gcactatctc gccaataaca cctggctgcc gctcggcccg tcactggttt ctcagcccat
     8521 caactggcgc gggcggcatc tggtctggta cttgctgctg tttgttatca gtctctggct
     8581 ccagttggga ttgccggacg aactgtcgcg ctttacgcca ttctgtctgg cgctgccgat
     8641 tatcgcgctg gcctggcact atggctggca aggagcgctg attgcgacgt tgatgaacgc
     8701 catcgcgctg atcgccagtc aaacctggcg cgatcatccg gtggatttat tactctcgtt
     8761 gctggtgcaa agtctgacag ggttgttact gggcgcaggc atccagcggt tgcgtgaact
     8821 taaccagtcg ctacaaaagg aactggcgcg caatcagcat ctggctgaac gtttgttaga
     8881 aaccgaagag agcgtgcgcc gcgacgtggc gcgtgagctg cacgacgata tcggtcagac
     8941 catcactgct attcgtactc aggcgggcat tgttcagcgg ctggcggcag ataacgccag
     9001 tgtgaagcag agcgggcagc tcatcgaaca actgtcgctg ggcgtttacg acgcggtgcg
     9061 ccgtttgtta ggacggttac gtccgcgcca gctggatgat ctcaccctgg agcaggccat
     9121 ccgctcactg atgcgggaaa tggagctgga agggcgcggc attgtcagcc atctcgaatg
     9181 gcgtatcgat gaatcagcat taagcgaaaa ccagcgcgtg acgctgtttc gtgtctgcca
     9241 ggaagggctg aacaacattg tgaaacatgc cgatgccagc gcggttacgc tgcaaggctg
     9301 gcagcaggat gaacggttga tgctggtgat tgaagacgat ggcagcggtt tgccgccggg
     9361 ttccgggcaa caaggttttg gcctcaccgg aatgcgcgag cgcgtaacgg cgctgggcgg
     9421 cacattacac atttcctgtc tgcacggcac gcgtgtcagc gtttctctac ctcaacgtta
     9481 tgtctaaggt tcgatgatgt tgccgtttct gaaagcgcct gccgatgcgc cattaatgac
     9541 tgataaacat gaaattgatg cccgttatcg ctactggcgt cggcatattc tgctgaccat
     9601 ctggctgggt tacgcgctgt tttacttcac gcggaaaagt tttaacgccg cggtaccaga
     9661 aatccttgct aacggcgtgc tcagccgtag cgatatcggc ctgttagcga ccctgtttta
     9721 cattacttac ggcgtgtcga agtttgtctc cggcattgtc agcgatcgct caaatgcccg
     9781 ttattttatg gggatagggc ttattgccac gggcatcatg aacattctgt ttggcttctc
     9841 gacgtcgcta tgggcgtttg ccgtgctctg ggtgctgaac gcctttttcc agggctgggg
     9901 ttcaccggtg tgtgcgcgtc tgttaacggc ctggtattca cgtaccgagc gcggcggttg
     9961 gtgggcatta tggaacacgg cgcataacgt cggcggcgca ctcattccca ttgtgatggc
    10021 agcgagcgcg ctgcattacg gctggcgtgc cgggatgatg attgctggtt gtatggcgat
    10081 agtcgtgggg atttttctct gctggcggct acgcgatcgc ccgcaggcgt taggtttacc
    10141 ggcggtcggt gaatggcggc acgacgcgct ggaaattgct caacaacaag aaggggcagg
    10201 gttgacgcgt aaagagatcc tcaccaaata tgtgttgctg aatccgtata tctggctgct
    10261 ttcgttttgc tatgtgctgg tctatgtggt ccgggcggcg atcaacgact ggggcaattt
    10321 gtatatgtcc gagacgctgg gcgtcgatct ggtcacggcg aatacggcag tgacgatgtt
    10381 tgaactgggc ggatttatcg gtgcgctggt agccggttgg ggctcggaca aattgtttaa
    10441 cggcaaccgt gggccgatga atttgatttt cgccgccgga attttgcttt cagtcggctc
    10501 gctgtggctg atgccatttg ccagctacgt gatgcaggca acctgcttct tcaccattgg
    10561 tttttttgtc tttggcccgc agatgttaat tggtatggcg gcggcagagt gttcccacaa
    10621 agaggcggca ggggcggcga cggggtttgt cggcttgttt gcttatctgg gggcgtcgct
    10681 tgctggttgg ccgctggcga aagtgctcga tacctggcac tggagcggat tttttgtggt
    10741 tatctctatc gccgccggga tttccgcact gctgttactg ccctttttga acgcccagac
    10801 accgcgtgaa gcgtgatgca tctcaccttt tcacttcata tccggcaaaa ctaagaaatt
    10861 ttccaggttt ttcttggacg ctatctcagg cctgatttgc tgctgatttt tacaatgcat
    10921 gcctcacgca ggtattcatt tcaggagtaa cccatgctgg ctttcctaaa ccaggttcgc
    10981 aagccgaccc tggaccttcc gctcgaagtg cggcgcaaaa tgtggttcaa accgttcatg
    11041 caatcctacc tggtggtctt tatcggctac ctgacgatgt acctgattcg caagaacttt
    11101 aacatcgcgc agaacgatat gatttcgacc tacgggttga gcatgacgca gctggggatg
    11161 atcggcctgg gtttctccat cacttatggc gtgggtaaaa cgttggtttc atactacgcc
    11221 gacggcaaaa acaccaaaca attcctgccg ttcatgctta tcctctctgc catttgtatg
    11281 ctgggcttca gtgccagtat gggcagcggc tcggttagcc tgttcctgat gattgccttc
    11341 tacgccttaa gcggcttttt ccagagtacc ggcggttcgt gcagttactc caccatcacc
    11401 aaatggacgc cgcgtcgtaa acgcgggaca ttcctcggtt tctggaatat ttctcacaac
    11461 cttggcggtg caggcgcagc aggtgtggcg ctgttcgggg caaattacct gttcgatggc
    11521 catgtcatcg gcatgtttat cttcccgtcg attatcgcgc tgattgtcgg ttttatcggc
    11581 ctgcgttacg gcagcgactc cccggaatct tatggcctcg gcaaagctga agaactgttc
    11641 ggcgaggaga tcagcgaaga ggacaaagag acagaatcta ccgatatgac caagtggcag
    11701 atctttgttg agtatgtgct gaaaaacaaa gtgatctggc tgctgtgctt cgccaacatt
    11761 ttcctctatg tggtacgtat tggtatcgac cagtggtcaa ccgtatacgc gttccaggaa
    11821 ctgaaactct ctaaagcggt ggcgattcag ggctttacgc tgtttgaagc tggtgcgctg
    11881 gtcggtacgc tgctgtgggg ctggctctct gacctggcga acggtcgccg tggcctggtg
    11941 gcctgcatcg cgctggcgct gattatcgcc acgctcggtg tgtatcaaca tgccagtaac
    12001 gaatatatct atctggcttc tctctttgcg ttgggtttct tggtctttgg cccgcaattg
    12061 ttgattggtg tggctgctgt tggctttgta cctaaaaaag cgattggcgc tgccgatggt
    12121 attaaaggca cctttgctta cctgattggt gacagctttg ccaagttagg tctgggaatg
    12181 attgccgatg ggacgccggt attcggcctt accggctggg caggcacctt cgccgcgctg
    12241 gatatcgccg cgattggttg tatctgcctg atggcgatag tggcggtaat ggaagaacgc
    12301 aaaatccgcc gcgagaaaaa aattcagcag ttgacagtgg cataaacgta actggtgact
    12361 tttgcccggc atgacgccgg gcttttttta ttattccgtg acttccagcg tagtgaaggc
    12421 aaacttctcg ccatcaaata gcccctgact ggttagtttt agcgcgggga tcactggcag
    12481 agaaagaaac gccatctgaa taaacggctc atcgggtaac ggaccgcatt cacgggcggc
    12541 ggctttcaag gcgtcaattt gttccgccag cgactgcgcc gtgtcggtgc tcatcagccc
    12601 ggcaatgggt aacggcagat gactttgtac ctggccgtta cgtaccacgc acagcccgcc
    12661 gccatcctga atcacctgat tgaccgccag cgccatctct tcggcactgc gaccgatcac
    12721 cacaatatta tggctgtcat ggctgactgt cgcagccagc gcgccttcat tcaggccaaa
    12781 gccgccgagc aaaccacagg ccggagccag ccgttgcccg taacgctcaa gtacggcaat
    12841 aaagcacaca tcatcgcgat caaaaccatt ttcgctgtag acgctggagc gggagtgcgt
    12901 aatcaattcg ttatggatga cgtcaatgac ccgatagcgt tttccgggcg tgaattgcag
    12961 ggcaaagtcg ctggcgggca atggtattgc catatggcgg ggcagacagc gccagtctcg
    13021 ccgactcttc cgcctgtaag gtttgcgcat caatcggttc gcctttcacc agtacctgct
    13081 gcaccgtgac cttacgcgca tcgctcaaca ggacgatatc ggcctgcttg ccgggtgcca
    13141 gtaagccgag gtgattcaga ccaaagtggc gcgccgtcga ccagctggcg acgcgatatg
    13201 ccacatgcag cggcacattg tgttgttcga tcaggcggcg aattaaggca tcgatatgcc
    13261 cttcatgggc gatctcccac gggttacggt catcggtaca gagcatgcat tgcgggctgt
    13321 taaattcgtt gatcaacggt gccagtgcgt tgagattgcg ggcagcggac ccttcgcgga
    13381 tcatcaacga catgccgagt tgtaatttcc ggcgtccttc ttccagctga taactttcgt
    13441 ggcagttttc aatacccgca gcaatatagg cgttaagttc tttgccaccc aaacccggac
    13501 agtgaccgtc cagcgtcagg tagcgaaatg catcgagttt atcgagcagc gcattctgcc
    13561 cgttaattac gccagggtag tccatcattt ctgcaaggcc ggtaacctgc ggatggtccc
    13621 gccaggcgag catctgttca agggtaaaac tggcaccgtt aacatcgcag ccttccagag
    13681 cgggcacgca agagctgacc tgtaagtact ggttttgcct tgcctgttcg gcacagcggg
    13741 caaaccaggc gaatccggct tctcccatca cgttgacgat ttcatgaggg tcgcaaataa
    13801 cggtcgtcag gccgcgcggc agggtagcgg tttcaaaagt gaccggcgtc atcatgctgg
    13861 attcaatatg caggtgagca tcaataaacc ctggcaccgc cgttgcgccg cgagcatcaa
    13921 tccgctgcaa agccggagca tcagcgtatt ctgcgccaac accggcaatg taacgtcctt
    13981 taatcacaat tgggctggaa atttctccgg cattgatcag gtcgagaata gagacattat
    14041 caataatata atcggcaaca gcgtcgccac gggaaacggc taacaattcc tggtattcag
    14101 cccggctaat gtgatgaaat ttatggttaa tggaattatt catatttttc tcattttgat
    14161 tttatcaatt tgattattat ttatcataaa gaaacgtcgc gagattatta aagaatatct
    14221 attaatgtgc aattgaaatg tgataattat cactgaaccg ttgttgtcat ttgttttaca
    14281 aaagcaaggg attaaatctc ctccaatgga caaaaaaatg aataatgaca ataccgatta
    14341 cgtgagtaat gaatcaggga cgctttcgcg attatttaaa ctacctcagc atgggaccac
    14401 cgtccgcaca gaattgattg cggggatgac cactttttta accatggtgt acattgtttt
    14461 tgtgaacccg caaatcctcg gcgcggcaca aatggacccg aaagtggtgt ttgttaccac
    14521 ctgtttgatt gccggtatcg gcagtattgc gatggggata tttgctaact tacccgtggc
    14581 gctggctccg gcaatggggc tgaacgcctt ctttgcgttc gtggtcgtgg gggcgatggg
    14641 gatctcctgg cagaccggga tgggcgcgat attctggggc gcaattgggc tatttttgct
    14701 cacgctgttt cgtatccggt actggatgat ctccaacatt ccattaagtt tacgtattgg
    14761 tatcaccagt ggaatcggat tatttattgc cttaatggga ttaaaaaaca ctggtgttat
    14821 tgtcgccaat aaagacacgc tggtgatgat tggcgattta agttctcacg gcgtgttgtt
    14881 aggtatttta gggtttttta ttataaccgt gttgtcatca cgtcattttc atgccgcggt
    14941 gctggtttct attgtggtga cgtcttgctg tggattattt ttcggtgatg ttcattttag
    15001 cggcgtctat tccattccgc ctgatattag cggcgtcatt ggtgaagtag atttgagcgg
    15061 cgcgttaaca cttgaactcg ccggtatcat tttctccttt atgctgatca acctatttga
    15121 ttcatcagga acattaattg gtgtaactga taaagcgggc ttaatagata gcaacggtaa
    15181 attccccaat atgaataagg cgctgtatgt tgatagcgtc agttcggtgg caggcgcatt
    15241 tatcggtacc tcgtctgtta ctgcctatat tgaaagtact tctggtgtgg cagtcggtgg
    15301 tcgcacgggg ctaacggctg tcgtggtcgg cgttatgttc ctgttagtta tgttcttctc
    15361 accgctggtg gcgatggttc ctccttacgc aaccgctgga gcgttaatct ttgttggcgt
    15421 gctgatgact tcgagcctgg cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc
    15481 gtttattacc acggtgatga tgccctttac tttctcgatc accgaaggga ttgcactcgg
    15541 ctttatgtcg tactgcatca tgaaagtgtg caccgggcgc tggcgcgatc tgaacctgtg
    15601 tgtggtggtg gtcgcagctc tgttcgcatt gaagattatt ctggtggatt agtaactggc
    15661 gagggtttcc agatatcatg agttctgatt acgcaggaga actcatgatc tggataatgc
    15721 tcgccacgct ggcggtagtg tttgtggttg gttttcgggt gctgacatcc ggggccagaa
    15781 aagcgattcg ccgtctcagc gatcggctga atatcgatgt cgtacccgtg gagtcgatgg
    15841 tcgatcaaat gggaaagtca gccggtgacg aatttttacg ttatttgcat cgcccggatg
    15901 agtcgcacct gcaaaacgcc gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg
    15961 atagcgaaca gaacctgctg caatggcacc ggattttaca aaaagcccgc ctttccgcgc
    16021 cgattaccga tgctcaggtc aggctggcgc tgggttttct gcgtgaaact gaacctgaaa
    16081 tgcaggatat taatgctttt cagatgcgct ataacgcgtt ctttcagcct gccgagggcg
    16141 ttcactggtt gcattgaaac cgtttcgtct tatcagccta cagaccgcgc ctgacctgtc
    16201 gggtggataa gacgttcacg ccgcatccgg taatctttgc cctgttccgt ttcgatctta
    16261 actccagctc aagcaacacc gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa
    16321 tgttaaagtg gtcggctttt cccctgaaac atgccacggg taacaccatg agtgaattta
    16381 ttgccgaaaa ccgcggcgcg gatgccatca cccgaccgaa ctggtcagcc gttttctcgg
    16441 tggtgttttg tgtcgcctgt ctgattatcg ttgagttttt gcccgtcagt ttgttgacgc
    16501 caatggccca ggatttaggc atttcggaag gggttgccgg gcaatcggtg accgtgaccg
    16561 cctttgtggc aatgtttgcc agtttgttta ttacccagac aattcaggcc actgaccgcc
    16621 gctacgttgt tattttgttt gccgttttgc tgacgctctc ctgcttgctg gtttcctttg
    16681 ctaactcatt cagtttgctt ttaatcggtc gtgcctgtct ggggctggcg ctgggcgggt
    16741 tctgggcgat gtcggcgtcg ctgaccatgc gtctggtacc gccgcgtacg gtgccgaagg
    16801 cgctgtcggt gatcttcggc gcggtttcta ttgcgctggt gattgccgcg ccgttgggca
    16861 gttttttagg cgagcttatc ggttggcgca atgtctttaa tgcggcggcg gtgatgggcg
    16921 tgctgtgtat tttctggatt atcaaatcat tgccttcact gccaggcgaa ccctcgcatc
    16981 agaaacaaaa tattttccgc ttattacaac gtccgggtgt gatggcaggg atgatcgcca
    17041 tcttcatgtc tttcgccggg cagtttgctt tcttcacgta tattcgcccg gtgtatatga
    17101 acctagcggg attcggcgtg gatggcttaa cgctggtgct gttgagtttt ggtatcgcca
    17161 gctttattgg tacgtcgctt tcgtcgttca ttcttaaacg ttcggtaaaa ctggccttag
    17221 caggcgcgcc gttaatactg gctgtgagtg cgttggtact gacgttgtgg ggaagcgata
    17281 aaatcgttgc taccggcgtg gcgattatct gggggctaac ttttgcattg gttcccgtcg
    17341 gctggtcaac gtggatcacc cgctcgctgg ccgatcaggc agaaaaagcc gggtctattc
    17401 aggtggcggt tattcagctt gctaatacct gtggcgcggc aatcggcggt tatgcgctgg
    17461 ataatattgg tctgacttcg ccgttgatgt tgtccggcac attgatgttg ctgac
//
LOCUS       DADKXM010000079        17446 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.080, whole genome shotgun sequence.
ACCESSION   DADKXM010000079 DADKXM010000000
VERSION     DADKXM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.080"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(12..71)
                     /gene="ibsA"
                     /locus_tag="JQR05_002570"
     CDS             complement(12..71)
                     /gene="ibsA"
                     /locus_tag="JQR05_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY5324715.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(343..399)
                     /locus_tag="JQR05_002571"
     CDS             complement(343..399)
                     /locus_tag="JQR05_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY5324716.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(671..727)
                     /locus_tag="JQR05_002572"
     CDS             complement(671..727)
                     /locus_tag="JQR05_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY5324717.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            1006..2253
                     /gene="mdtA"
                     /locus_tag="JQR05_002573"
     CDS             1006..2253
                     /gene="mdtA"
                     /locus_tag="JQR05_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY5324718.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSPFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTL
                     LSLNNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            2253..5375
                     /gene="mdtB"
                     /locus_tag="JQR05_002574"
     CDS             2253..5375
                     /gene="mdtB"
                     /locus_tag="JQR05_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY5324719.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDQVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5376..8453
                     /gene="mdtC"
                     /locus_tag="JQR05_002575"
     CDS             5376..8453
                     /gene="mdtC"
                     /locus_tag="JQR05_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY5324720.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPKALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8454..9869
                     /locus_tag="JQR05_002576"
                     /pseudo
     CDS             8454..9869
                     /locus_tag="JQR05_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9866..11269
                     /gene="baeS"
                     /locus_tag="JQR05_002577"
     CDS             9866..11269
                     /gene="baeS"
                     /locus_tag="JQR05_002577"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY5324721.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            11266..11988
                     /gene="baeR"
                     /locus_tag="JQR05_002578"
     CDS             11266..11988
                     /gene="baeR"
                     /locus_tag="JQR05_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY5324722.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAVSYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12179..12511
                     /locus_tag="JQR05_002579"
     CDS             12179..12511
                     /locus_tag="JQR05_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY5324723.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12658..14019
                     /locus_tag="JQR05_002580"
     CDS             12658..14019
                     /locus_tag="JQR05_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY5324724.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14361..14678)
                     /locus_tag="JQR05_002581"
     CDS             complement(14361..14678)
                     /locus_tag="JQR05_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324725.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            15084..15983
                     /gene="yegS"
                     /locus_tag="JQR05_002582"
     CDS             15084..15983
                     /gene="yegS"
                     /locus_tag="JQR05_002582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY5324726.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAITIDMAQVNKQTCFINMPTGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(16065..16844)
                     /locus_tag="JQR05_002583"
     CDS             complement(16065..16844)
                     /locus_tag="JQR05_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5324727.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(16944..>17446)
                     /locus_tag="JQR05_002584"
     CDS             complement(16944..>17446)
                     /locus_tag="JQR05_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5324728.1"
                     /translation="VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVL
                     RELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELT
                     VIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIARNAMPG
                     KVLLIP"
BASE COUNT         3961 a         4671 c         4614 g         4200 t
ORIGIN      
        1 ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag tatgatgacg
       61 tgcttcatca taaccctttc cttatttaag gccccttcct cgggaggggc tttcccgtgt
      121 cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc tgaatgcagt ctatcgctaa
      181 cgcgtgaaca aaatactttc ctctgtgatt attgcgtaac aactcgcaaa gaatatttgc
      241 tggcaggatc gcagactaca aagcctacgg attgacaatc ttatcgtgaa ggcataattt
      301 caggagtgag ggtagagcgg ggtttccccc gccttggtag tcttagtaag cggggaagct
      361 tatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc
      421 cccttcctcg ggaggggctt tcccgtttca gcgtcccgct gaaatcggcg gcttacctcc
      481 tttcgccctg aatgcagtct atcgctgact tgcggataaa atagtttcca gtgtgattat
      541 tgcgtaacgt ctcgcaaaga atatttgctg gcaggatcgc agactgcaaa gcctgcagat
      601 tgacaatctt atcgtgaagg catactttca ggagtgaggg tagagcgggg tttcccccgc
      661 cctggtagtc ttagtaagct ggccagctaa tgactaagag caccacgatg atgagtagct
      721 tcatcatgac cctttcctta tttatggccc cttcctcggg aggggctttc ccgtttcagc
      781 gtcccgctga aatcggcggc ttacctcctt tcgccatgca tgcagtctat cgctaacgcg
      841 tagataaaat agtttcctgt gttattactg gatgcgtgct cgcaaatgtg cccgccattc
      901 agacgattcc cgatagtgtt tcataattcc tccatttttc tcccttattg gctggctaca
      961 ctagtatcat tccgcgaaac gtttcaggaa gagaaactct taacgatgaa aggcagttat
     1021 aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg ccatcgccgc attctggttc
     1081 tggcaaggcc gcaatgactc ccggagtgca gccccagggg cgacgaaaca agcgcagcaa
     1141 tcgccagcgg gtggtcgccg tggtatgcgt tccggcccat tagccccggt tcaggcggcg
     1201 accgccgtag aacaggcagt tccgcgttac ctcaccgggc ttggcaccat taccgccgct
     1261 aatactgtta cggtgcgcag ccgcgtggac ggtcaactga tggcgttaca tttccaggaa
     1321 ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagccc gttcaaagtt
     1381 gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc taacgcccgc
     1441 cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg tcaggagctg
     1501 gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc
     1561 gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc
     1621 gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac caccggaatt
     1681 gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga aagcgatatc
     1741 gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc ctgggatcgc
     1801 accaattcga agaaattaag tgaaggcacg ctgttaagcc tcaataacca aatcgatgcc
     1861 acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc
     1921 aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca
     1981 acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac
     2041 aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt
     2101 gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg
     2161 gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc
     2221 cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag
     2281 gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga
     2341 tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg
     2401 actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat gtcatgacct
     2461 ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt
     2521 cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta acattaccgc
     2581 tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga
     2641 gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga
     2701 cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc
     2761 gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc
     2821 agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca
     2881 gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg
     2941 acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt
     3001 atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc gatgtcgcaa
     3061 ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa gaacaggcca
     3121 ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcattc
     3181 ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg acagtgcttt
     3241 ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg
     3301 ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca
     3361 ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg gtgtttctcg
     3421 atttttcaat caataacctg acactgatgg cgttaactat cgccaccgga ttcgtggtcg
     3481 atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc gaaaaaccgt
     3541 tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct
     3601 cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc
     3661 gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc
     3721 tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa cagaaccgct
     3781 tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg
     3841 cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta
     3901 gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac aacggcatta
     3961 ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcgacaac
     4021 gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg
     4081 gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac ctcaaaccgt
     4141 tggatgaacg tgatgatcag gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata
     4201 aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc gatactcagg
     4261 tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct
     4321 gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat gtctccagcg
     4381 actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc
     4441 tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt cagcgactga
     4501 tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac aataccgaaa
     4561 ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac ggtggcgtgg
     4621 tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc atcaaccatc
     4681 tggatcagtt cccggtaacg accatctcct ttaacgtgcc ggataactat tcgctgggcg
     4741 atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca
     4801 cgcaattcca gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga
     4861 ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc
     4921 acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg ctggcattaa
     4981 tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta
     5041 ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag
     5101 gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga
     5161 tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg
     5221 cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc agccaggtgc
     5281 tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca ttgtggacca
     5341 aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt
     5401 accgccctgt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggtt
     5461 tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg
     5521 cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc
     5581 gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg ctcggcagca
     5641 cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg cgtgatgtgc
     5701 aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc cgcccgacct
     5761 atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt
     5821 attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa
     5881 tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg cgcgtcgggc
     5941 tgaacccgaa ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc actgccatca
     6001 gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga
     6061 tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt attcactaca
     6121 acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gaccgactca gtgcaggatg
     6181 tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaactgc
     6241 cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca gagttgcagg
     6301 agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg
     6361 cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg
     6421 tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg gtgccggttt
     6481 cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aatctttcgc
     6541 tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg gtgctggaaa
     6601 acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg caaggtactc
     6661 gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc
     6721 tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg
     6781 tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg tgcggctgga
     6841 tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc cgcatgttgg
     6901 tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat acccgtctgg
     6961 tgggcgtggt gctgcttggc accattgcgc tgaatatctg gctgtatatc tcgatcccga
     7021 aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga
     7081 gtatttcgtt tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg
     7141 atccggcagt ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga
     7201 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
     7261 gtctgcgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag
     7321 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
     7381 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
     7441 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
     7501 accgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata
     7561 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
     7621 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
     7681 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
     7741 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc
     7801 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
     7861 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga
     7921 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
     7981 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
     8041 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctaatag
     8101 ggatcatgct attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc
     8161 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
     8221 tgcgttttcg cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg
     8281 tattgtcggg cggtgacggc tcggagctgc ggcaacccct ggggatcacc attgtcggcg
     8341 gtctggtgat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
     8401 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
     8461 atcttcccga cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt
     8521 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
     8581 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
     8641 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
     8701 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
     8761 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
     8821 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
     8881 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt
     8941 ggcactggat ctttttgatc aacattccag tggggattat cggtgcgatc gccacattga
     9001 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
     9061 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
     9121 cgccgctggc gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc
     9181 tgcacgccag aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct
     9241 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
     9301 tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
     9361 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc
     9421 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
     9481 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
     9541 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
     9601 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
     9661 gtatcggcgt caccatcgcc gggctgttgc tggggctttt tggttcgcag catgtcagcg
     9721 tcgacagcgg caccacacaa accgtcttta tgtacaccta gcttagcatg gcgtttatca
     9781 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
     9841 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact
     9901 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
     9961 tatcagtttt gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca
    10021 gctgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg
    10081 caacaacgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    10141 taaacccggc ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca
    10201 aaacaacaaa gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    10261 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    10321 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc
    10381 cctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc
    10441 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    10501 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    10561 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    10621 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    10681 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    10741 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    10801 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    10861 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    10921 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    10981 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    11041 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    11101 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    11161 cattgttgaa gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    11221 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    11281 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    11341 ctcattgatt atctgcgtgc tgtgagctat gcgccgacgc ttatcagcca cggcgatcag
    11401 gtactggcgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    11461 ggcaccgatg gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg
    11521 atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat
    11581 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    11641 cgccgttgta aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc
    11701 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    11761 tttcgtctgc ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    11821 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt
    11881 aaaaatctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    11941 tacggcgttg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    12001 tactttgtta gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt
    12061 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    12121 cagtaaacag cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat
    12181 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    12241 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    12301 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc
    12361 aagcaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    12421 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    12481 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    12541 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct
    12601 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    12661 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    12721 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac
    12781 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    12841 ttttatgtcg tggtcaacat tgcaccacac aacgccaagc tgaaaacctt tatccgtgac
    12901 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    12961 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    13021 gtgaactggg cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct
    13081 cgcgagctgt cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc
    13141 gagatcttcg ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc
    13201 tatatcaaca agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac
    13261 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    13321 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    13381 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    13441 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    13501 ggcgtgcatt cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc
    13561 gcacaggttt accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc
    13621 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    13681 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    13741 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    13801 aaattctccg ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc
    13861 cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat
    13921 actgtgtggc tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat
    13981 ttctccgggg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    14041 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    14101 ttcgctgaaa aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    14161 tgtaatctcc cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc
    14221 tgataaccgt cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc
    14281 acgtaacggt atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg
    14341 acgcttacca aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    14401 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    14461 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
    14521 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    14581 gcaagaccaa atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga
    14641 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac
    14701 caacgacact ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat
    14761 tataataaat gttctgtaca acatttccta cataagtagg aattacggac attgaggccc
    14821 ttcagggtaa ctccatgggg gctttaatat attatattga agatgccact gtttagttga
    14881 atattaggta tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc
    14941 ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc
    15001 tccgactttc atcctgttct gactccacct tttgttttct gctctacact atctacagac
    15061 caatcataaa ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg
    15121 gcaaaagtac tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa
    15181 tgacgatcca tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg
    15241 cccggaagtt gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag
    15301 tttctacggc gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat
    15361 taggaaccgc caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc
    15421 tgaaactggc aattgccggt aatgccatta cgatagatat ggcgcaggtc aacaaacaaa
    15481 cctgttttat taatatgccg acaggcggat ttgggacgcg tattaccaca gaaacgccgg
    15541 aaaaattaaa agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg
    15601 atactctgca accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg
    15661 ccctggtcat tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga
    15721 acgcgttaat taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc
    15781 cggctctcgt atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt
    15841 cgtcgtggtt tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt
    15901 tgagtgggca aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac
    15961 cagattgtcc attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa
    16021 tgccggatgc ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat
    16081 tttacccgtg tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca
    16141 ataatgtcaa ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat
    16201 gaatcgagaa cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa
    16261 ccttcattaa aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt
    16321 aaatcggcat taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt
    16381 ttatggcgga ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca
    16441 aacgctgccg ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg
    16501 gcgataagca ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt
    16561 ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta
    16621 aacccgactt cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca
    16681 tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct
    16741 tccgaggcag caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact
    16801 aattggatga tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt
    16861 gtggctagtg taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc
    16921 cagcatcacg ctggcccgcg gtttcatgga atgagcaaca ctttgcccgg catggcatta
    16981 cgagcgatgt cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat
    17041 ggatccaggc ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc
    17101 ggccaagggc tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt
    17161 ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt
    17221 tgggcatgag gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc
    17281 tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc
    17341 atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct
    17401 gagctaatgt cgatcgccgt cacactcttt gctcccagcg cgacag
//
LOCUS       DADKXM010000080        17379 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.081, whole genome shotgun sequence.
ACCESSION   DADKXM010000080 DADKXM010000000
VERSION     DADKXM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.081"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(35..766)
                     /gene="rlmB"
                     /locus_tag="JQR05_002585"
     CDS             complement(35..766)
                     /gene="rlmB"
                     /locus_tag="JQR05_002585"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY5324729.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     TGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(946..3387)
                     /gene="rnr"
                     /locus_tag="JQR05_002586"
     CDS             complement(946..3387)
                     /gene="rnr"
                     /locus_tag="JQR05_002586"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY5324730.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKSGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3426..3851)
                     /gene="nsrR"
                     /locus_tag="JQR05_002587"
     CDS             complement(3426..3851)
                     /gene="nsrR"
                     /locus_tag="JQR05_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY5324731.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4056..5354)
                     /gene="purA"
                     /locus_tag="JQR05_002588"
     CDS             complement(4056..5354)
                     /gene="purA"
                     /locus_tag="JQR05_002588"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY5324732.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5458..5655)
                     /locus_tag="JQR05_002589"
     CDS             complement(5458..5655)
                     /locus_tag="JQR05_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY5324733.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5737..6741)
                     /gene="hflC"
                     /locus_tag="JQR05_002590"
     CDS             complement(5737..6741)
                     /gene="hflC"
                     /locus_tag="JQR05_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY5324734.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWCISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6744..8003)
                     /gene="hflK"
                     /locus_tag="JQR05_002591"
     CDS             complement(6744..8003)
                     /gene="hflK"
                     /locus_tag="JQR05_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY5324735.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8089..9369)
                     /gene="hflX"
                     /locus_tag="JQR05_002592"
     CDS             complement(8089..9369)
                     /gene="hflX"
                     /locus_tag="JQR05_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY5324736.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSTAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9445..9753)
                     /gene="hfq"
                     /locus_tag="JQR05_002593"
     CDS             complement(9445..9753)
                     /gene="hfq"
                     /locus_tag="JQR05_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY5324737.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9839..10789)
                     /gene="miaA"
                     /locus_tag="JQR05_002594"
     CDS             complement(9839..10789)
                     /gene="miaA"
                     /locus_tag="JQR05_002594"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY5324738.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10782..12629)
                     /gene="mutL"
                     /locus_tag="JQR05_002595"
     CDS             complement(10782..12629)
                     /gene="mutL"
                     /locus_tag="JQR05_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY5324739.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIAPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12639..13976)
                     /gene="amiB"
                     /locus_tag="JQR05_002596"
     CDS             complement(12639..13976)
                     /gene="amiB"
                     /locus_tag="JQR05_002596"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY5324740.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQEQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13995..14456)
                     /gene="tsaE"
                     /locus_tag="JQR05_002597"
     CDS             complement(13995..14456)
                     /gene="tsaE"
                     /locus_tag="JQR05_002597"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY5324741.1"
                     /translation="MMNRVIPLPDEQVTLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14428..15975)
                     /gene="nnr"
                     /locus_tag="JQR05_002598"
     CDS             complement(14428..15975)
                     /gene="nnr"
                     /locus_tag="JQR05_002598"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY5324742.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15974..17113
                     /gene="queG"
                     /locus_tag="JQR05_002599"
     CDS             15974..17113
                     /gene="queG"
                     /locus_tag="JQR05_002599"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY5324743.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17096..17149)
                     /locus_tag="JQR05_002600"
     CDS             complement(17096..17149)
                     /locus_tag="JQR05_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324744.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3936 a         4958 c         4368 g         4117 t
ORIGIN      
        1 ccaaatctgg atggcttttc ataattcaga ggaactagct gcgctgacga accgcttcaa
       61 ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct gtcattggga
      121 tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca ccttccgcgc
      181 ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga tccgcctcgc
      241 ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg cgcgcaaggt
      301 tagtcacccg aatcagcgga acgctttctg ccgcgccgca ggctactttt ttcgccgtag
      361 cgttgagctg tgcggagcga tctttcggca caatcaccgc atgaacgcct gcggcgtccg
      421 cactacgcag gcacgcgccg aggttgtgcg gatcggttac gccgtcgagg atcagcagga
      481 acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac tgacgtcctg
      541 gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg tcgagatatt
      601 ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc agcggtaaca
      661 gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca ggggcgcgct
      721 ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat gtactcgttg
      781 tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc aatatattga
      841 atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg
      901 cattttgtca gcaatctaac cctcttcttt taaagagggt attgatcact ctgccacttt
      961 tttcttcgcc gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg
     1021 ctttttcgct tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg
     1081 gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct tgccgccttt
     1141 tttgcctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc
     1201 gcgttcactg gagatcagac taaagtcgat tttgcgctcg tccatattaa ccgcttcgac
     1261 gcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattccc ccatcaggcg
     1321 ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat ggaccagacc
     1381 atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc tggaaattac
     1441 gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc agtcagcgac
     1501 atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa
     1561 catctcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg
     1621 ctctttcgcc agcagatatt taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat
     1681 cggcgaagta aagtgcgcat aggactgcaa tgccagaccg aagtgaccac ggttttctgg
     1741 atcgtaaatc gcctgtttca tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg
     1801 acggtcggca accgactcca gcagttccgc gtagtcacgc ggttccggct tgttaccacc
     1861 cggcagctcc agccccagct ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct
     1921 cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga aacgcgccgc
     1981 cgagatattc gccagaatca tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt
     2041 ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc
     2101 aaatgagatc ccaccgcgtt cttcacgggc tttatccagc actttataga ggttatgcaa
     2161 ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat cgccctgcag
     2221 aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa
     2281 tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca tacacaggcg
     2341 gtctacctgc gggttgagcg aacacaggcc gttagagagc acttccggca gcatcgggat
     2401 aacctgcgaa gggaagtaca ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt
     2461 tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc
     2521 gcgttttttc tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt
     2581 gaccagcggt aaatcgcgca aatcgacacg gcccgatttt gcttcttccg gcacttcttc
     2641 tttcagcccc gcaacctgtt gctcaacagc ctgcggccag atatacggaa tttcatgggt
     2701 acgcagagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca cttcgacgat
     2761 tttacccacc gctttggtgc ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa
     2821 gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc tcagacggct
     2881 atcgtcagga accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa caatctggct
     2941 ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac ggtcagcacc
     3001 cagcggctgc gccagcacct gatcgccatg aatgcaggtt ttcatctgct cgctggagag
     3061 atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac ggtggccaat
     3121 aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac gacgagtgaa
     3181 gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt caagctgctc
     3241 ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt tttcacgttt
     3301 ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt tttcagcttc
     3361 gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc tgatgaagat
     3421 tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac caaatcggca
     3481 agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc
     3541 aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag cgataagggc
     3601 tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc caggcgaatg
     3661 ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg attgattatt
     3721 ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat actggtcatc
     3781 cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc agtgaaactc
     3841 gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat attgatgata
     3901 aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat
     3961 ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc acactgttag
     4021 cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg gtcgcgcaga
     4081 atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggcac accggtcagc
     4141 tcttcaatac gcttgatgta gttcagcgcc gcctgcggca ggccgctacg atctttcacg
     4201 ccgaaagtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac acctttccag
     4261 tcgtcagctg ccagcggagt ggtagtcact tcgcgaccat ccggcatacg gtaagccacg
     4321 cagagtttaa cctctttcag gccatccaga acgtccagtt tagtcaggca gaagccagac
     4381 agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc ggtacgacga
     4441 cgacgacccg tagttgcacc gaattcgtta ccctgcttgc agaggaactc gccagtttca
     4501 tcaaacagtt cggccgggaa aggacctgca cctacacgag ttgagtaagc tttgaggata
     4561 cccagaacgt aatcaacata acgcgggccc aagccggaac cggtcgccac gccaccagca
     4621 gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag cagcgtaccc
     4681 tgcgcacctt cgaacatgac gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa
     4741 acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc cagaactttc
     4801 tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg atattccatc
     4861 acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg
     4921 cgacgtgcca ctttatcttc ataagcaggc ccgataccac gaccggtggt gccgatcgct
     4981 ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc
     5041 ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc ttccagttct
     5101 ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat gatgctggtt
     5161 acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc accgttgatt
     5221 acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt agcccgttca
     5281 gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc cagtacgacg
     5341 acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt
     5401 ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt tttttgttca
     5461 gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc
     5521 aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct tcttccatgc
     5581 cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa gcgccagcca
     5641 gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat ccctgaggat
     5701 gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt
     5761 cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct gattgccaga
     5821 gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg gatctttact
     5881 gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca taatgcggcc
     5941 ttgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg cgcgcagttt
     6001 ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac gctcggcgcg
     6061 catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct tgatacgcac
     6121 atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg gaactttgcc
     6181 cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg ctgccggggt
     6241 agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt
     6301 cagacgacca cgggaatcgg tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg
     6361 cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt cgccaccacc
     6421 cgttgccagg tagtaacggc tgaaatcgct gatgcaccat ttgatgtaag agtcgacgat
     6481 caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg
     6541 tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac caggctcata
     6601 aaccaaaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg taataccgcg
     6661 ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga tgatgataat
     6721 cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg
     6781 ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt gcttgctcca
     6841 ctggttgtgg aggaagaggc tggcggcaga cgcagcagat tgctggcacc gttatcgctc
     6901 ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac catcaggttg
     6961 ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc catcgtctcg
     7021 atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag aagtttagca
     7081 aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc acgcgcctct
     7141 tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt atacgcttct
     7201 gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct
     7261 tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt gatacccatg
     7321 tcatacggac gaatcgtctc ttccagttcg cgctgagtat cgctacgaat cacggtacga
     7381 ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag ggcgctgtcg
     7441 gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt ttccggattg
     7501 gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga cgtcagcatc
     7561 acaccagaag cggccagttc acgcacggct tccacgttca ccggtttgac ttcgtcgata
     7621 aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc aaagcgtgtt
     7681 accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca gataatgaca
     7741 atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg gccttgcgat
     7801 gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag ctttttgctc
     7861 agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca
     7921 tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta
     7981 ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt gatccctgtt
     8041 gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt agatcaggta
     8101 atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg gcatacgaac
     8161 ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct gaagctgata
     8221 aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct gcgccacctc
     8281 gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc cggtctgtgc
     8341 ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc
     8401 ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag cgtcgatctc
     8461 ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat
     8521 gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa atgccgccac
     8581 cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg tttcaccgac
     8641 atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc
     8701 gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt tggtatatcc
     8761 caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct gctcacgctg
     8821 cttttcaact ctttccaggc gcgactgtat ctgcacgatg cgattacgca acaaacgacg
     8881 gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt tctgtctttc
     8941 aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac
     9001 ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt
     9061 gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg ccgtgctcag
     9121 ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg caatttcaac
     9181 tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac cggtaatcac
     9241 ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgga ggtcttccat
     9301 atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag catcataacg
     9361 gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc gctggctccc
     9421 cgtgtaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt gcgcggaagt
     9481 attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg cgttgttact
     9541 gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt aaaccatctg
     9601 gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt gcccttgcag
     9661 cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca gtgcgttcag
     9721 gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata tgcttatttg
     9781 tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt gtacacattc
     9841 agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct ggtttttcac
     9901 tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc ttcgccaact
     9961 gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg ccttcaaggt
    10021 aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta tgcaaatctc
    10081 ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc tgatgaaaac
    10141 gctgctcaat gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg aactgatgca
    10201 cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt ttacccgaaa
    10261 tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga atccttgctg
    10321 ccgcaaccgg atctacctcc tgaagttgac gatgcaatga ctcccaacct tgctctgccg
    10381 cctgttgctc aattctggcc cgcacttccg ggtctgccga cggtagcggc gacaaccctt
    10441 ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc cgccccgccg
    10501 cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc gagtaagcct
    10561 gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct tcagcgttcg
    10621 gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg cttatcaact
    10681 ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag gccgtcggcc
    10741 ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc tttcagggct
    10801 tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt tttcacaagt
    10861 tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat tgaccactgc
    10921 gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg ttcgaatacg
    10981 gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt ttgttggcgt
    11041 aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa atcaataccc
    11101 aattccgcta gggcagactg cgctttttct aatgccgatt tttcttcgcc agaaactttt
    11161 agccgcaacg gaatcagcag cggctgggcg caaacgggcg cttcacccgg cgtcaattgt
    11221 gcctgacgca gccaacgttc tgccactggc aaggataaaa gtgaaatgtt gccatcgcgc
    11281 tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact ctgactgttc
    11341 gccgcaagtg caggttcctg cggttccggc gcttttggtt tttgcatcgg cgcgggcgtt
    11401 tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt agcctggctg cgcattcggc
    11461 cagggggcag ccggacgact gcctgaggcg ggcgcaggag agtagcgcgg agctaccggc
    11521 tcacgagctg ccggttctgc aaagtgattg cgccccgccg ccacgcggtt ttccgggatg
    11581 gcacgcggtg cgggttgtgg ttcatcgtcc agcggtagcg gcgtttccag ttgctgttgt
    11641 agcacgctca gcacgccctg atagataaaa tcatgcacca gacgcgactg atggaaacgc
    11701 acttcgtgtt tggcggggtg tacgttgacg tccacctgat gcggggcgat ctccagatac
    11761 aacacaaatg ccggttgctg atcggccccc agtttgtctt cgcaggcctg gcggatcgca
    11821 tgattgatca ggcgatcgcg catcatgcga ccattcacgt agcaatactg aatttccgcc
    11881 agtgcgggcg tagtgtgatt tggatcggcc acccagccgc gcagggtgag atcgccgtgt
    11941 tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc gcctaagcgc
    12001 cgttcttttt gcccgccttc cggcactgcg cggtactgac gcacaatttt accgttatgc
    12061 gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat ttcatcaata
    12121 tggctaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt gttgtagaac
    12181 agatccagca cctccagcgt cgttcccacc ggatgcgccg ccggtttaac cgtcacgtcc
    12241 atgtcgcgtc cttcggcata ggcctgccag gcttcctgct gttctgcggt gcgtgaagtg
    12301 agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa gcccaggctg
    12361 ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg agccagcgcc
    12421 agcgccagct catctttttt gataccgcag ccgttatcac gaatgcggat aagtttcgcc
    12481 ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct gttttccact
    12541 agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat ctggttcgcc
    12601 agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag cgtgcgatct
    12661 ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct gcggcgcaga ttgcatcgga
    12721 tgcgcaagga agtaattgcg caggcctttg taaatggctt ctgccagctg ttcttggtaa
    12781 tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt ttcgaccagt
    12841 actgatggga tatccggcga gcgcagaacg ccaaggctgg cgtgttctgg tcgacgttta
    12901 tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc ataccctacc
    12961 cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg gtcagactga
    13021 ctgttcgcca gcacatcacc cgccccaccc agcagctccg actgtttctc gtgctgctcc
    13081 agccagctgg ccatttcact gttggcgcga cggttagaga gcacccatac ggaagcgcca
    13141 gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt ggcgttttgc
    13201 ttacgtgcca catcactgcg ccccatcacc gagataaagt aatccccgtc acgggttaaa
    13261 acgcctttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc aatggcgatg
    13321 gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg accgccgtgt
    13381 ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg cgctgccgga
    13441 cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt tttaaacgga
    13501 ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac gcgtttcgca
    13561 accacgggcg gcggaggagg cggtggcggc acatcggcgt taatcgtaaa gacgacagtg
    13621 taattgctgc cattctgccg cttcaccgct tcggttttac cgttttcggt aagatcgacc
    13681 accagccgta gcgtttgtgc atctttaggc gttccagagc gaatcgcctt caccagatta
    13741 ttgccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat atcgagcgcc
    13801 acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa actcaacgtt
    13861 atccgcgcct gttgattacc gttagaaacc tgaatatcag agagcgtcgc ggcacccacc
    13921 ggcgtgcaca gcagcagcag cgtcgctacc aaccaatttc tgatgcgata catcatcccg
    13981 ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc agaggaaacc
    14041 gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc gacatccggg
    14101 tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc atcgttggca
    14161 aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg gtacaaatca
    14221 aagtgataga ccattaagtt gtcgagcgta tagggttcga ccagcgtata agtggggctt
    14281 ttgacattac cctgatgacc cagagcctgt aaaaagcccc ggctaaaggt ggttttacct
    14341 gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt cgctacccgc
    14401 tcgcccaggt ctaatgttac ctgctcatca gggagcggaa ttactcgatt catcatggtt
    14461 tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat cggttgccag
    14521 catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt gcgcgacaca
    14581 gcctgcacag gctgcatcat acggcgacag tttttgccca agcaatgcgc caataatacc
    14641 agagagcaca tcgcccatgc cgccgctcgc catgcctgca tttccggcat caataatgcc
    14701 taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg ctacgccgcc
    14761 ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt cagcgacgga
    14821 acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat tgtgacgctt
    14881 atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg gtttgcgaaa
    14941 attctcaact ttttgcaggg cttttttccc ccactcttgc tggcccagac cgggaccaat
    15001 caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatagtca gttcatgcac
    15061 catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg tcagtactcg
    15121 gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag cccccgccgt
    15181 gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg aagtcgggcg
    15241 acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt tcgtctcctg
    15301 acctgccagc cagctatcca gccccagtga gtcaaaatgc agttgtcctg taacatcccg
    15361 cgcttttcca gtgagcaagc ctagtttcag cgcaataaaa gtgatggtgt gatcggcgtt
    15421 gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga tatcaaccgc
    15481 cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg attcgcgggg
    15541 cgcttgctgc aagccggtac cgagcagcgc atcaacaatc agatctaccg attcgggcca
    15601 gacaatattc gaagcatgga tctcgccacc cgcgtttaac catgcttcgc gtgccagcgc
    15661 ggcctcttcc ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct caatgccgac
    15721 cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat gaccgcacag
    15781 caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg cggcctcgcc
    15841 agcgcgaagc atcagctcat agagtgtgag ccccagcaca tctgccgcct cgcgttctcc
    15901 gcggcggata tcgtcggcgt accagacggt gtgtggtata cttacggggt ttttcttcat
    15961 tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa aaattaaaca
    16021 gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc tcagcgagtc
    16081 cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg actggatggc
    16141 acgtcacggt atgctgcgcg ctcgccccca tgagttatta cccggtacgc tgcgtgtaat
    16201 cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc tgaaaaaccc
    16261 caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac ttctgcgcaa
    16321 ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga attttagacc
    16381 gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg ggctcggctg
    16441 gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct ttttaggtga
    16501 attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat gcggcaaatg
    16561 cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata ccgtcgatgc
    16621 tcgccgctgt atctcttatc tcaccatcga acttgaaggg gcgatcccgg aagagttgcg
    16681 accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct gcccgtggaa
    16741 tcgctattcg caactcacta cagaagacga tttcagcccg cgtaagccgc tacacgcacc
    16801 ggaactcatt gagttatttg cctggagcga agagaagttt ttaaaagtca cggaaggatc
    16861 ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg cattaggcaa
    16921 tgccccttgg gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg agcacccact
    16981 tctcgatgag cacatagcgt gggcgattgc gcagcaaata gagagacgaa atgcgtgcat
    17041 agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa aagggttacc
    17101 gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt gcaattcaag
    17161 acgaataaaa tcgtcaagtg atcgactcga caaaacggaa tcttgatttt taacttattt
    17221 atcaataggt ataaaaaata ccattcaccg tgcgaagttt ctttgaaatt aagattacag
    17281 cctgaggctg tggataactc tgtttacaag agtttgtcag agacaacaaa aaccagcaat
    17341 aacgcatcgc gtcgctgtgg atattttatt gagagaaga
//
LOCUS       DADKXM010000081        17306 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.082, whole genome shotgun sequence.
ACCESSION   DADKXM010000081 DADKXM010000000
VERSION     DADKXM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.082"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JQR05_002601"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JQR05_002601"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY5324745.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQVIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JQR05_002602"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JQR05_002602"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY5324746.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRALRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JQR05_002603"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JQR05_002603"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY5324747.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JQR05_002604"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JQR05_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY5324748.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JQR05_002605"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JQR05_002605"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY5324749.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JQR05_002606"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JQR05_002606"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY5324750.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="JQR05_002607"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="JQR05_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY5324751.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JQR05_002608"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JQR05_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY5324752.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVL
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JQR05_002609"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JQR05_002609"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY5324753.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JQR05_002610"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JQR05_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY5324754.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /locus_tag="JQR05_002611"
     CDS             complement(14846..15541)
                     /locus_tag="JQR05_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY5324755.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPYKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="JQR05_002612"
     CDS             complement(15664..16701)
                     /locus_tag="JQR05_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5324756.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..>17306
                     /locus_tag="JQR05_002613"
     CDS             17034..>17306
                     /locus_tag="JQR05_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="YhhY; regulated by the fur regulator; unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5324757.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWK"
BASE COUNT         4249 a         4742 c         4313 g         4002 t
ORIGIN      
        1 cctttggccc cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat
       61 atgatcagcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgacc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaagag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaagacc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgca ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg
     5041 atgcagttag acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacaac cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg
     5701 agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa
     5761 gagtaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccactaggag cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata
     7741 gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagcatc ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 acgataaatc atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
     8281 cagcgtgttc cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg
     8641 ccaggaaccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat
     8881 gccgctctct ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcacg cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gagcccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 agagtagttc agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa
    12361 gttaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccaag
    12481 caccaggaac gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgctg
    13261 catagcaaac gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatatggc accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc gagcaataat
    14101 ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtaacat tgccttttac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt
    15061 ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatacgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcaca ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg
    16201 cggcagtcca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 gacaatctct cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc
    16321 aaagcgacga ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag
    16381 ctctttcgcc tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa
    16501 cttaacatcg ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgaaacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaag
//
LOCUS       DADKXM010000082        17214 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.083, whole genome shotgun sequence.
ACCESSION   DADKXM010000082 DADKXM010000000
VERSION     DADKXM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.083"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            22..417
                     /locus_tag="JQR05_002614"
     CDS             22..417
                     /locus_tag="JQR05_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5324758.1"
                     /translation="MRWEDIRDGRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNL
                     KKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGS
                     DFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            653..1471
                     /locus_tag="JQR05_002615"
     CDS             653..1471
                     /locus_tag="JQR05_002615"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY5324759.1"
                     /translation="MTTRVIALDLDGTLLTLKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1472..2530)
                     /gene="modC"
                     /locus_tag="JQR05_002616"
     CDS             complement(1472..2530)
                     /gene="modC"
                     /locus_tag="JQR05_002616"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY5324760.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2533..3222)
                     /gene="modB"
                     /locus_tag="JQR05_002617"
     CDS             complement(2533..3222)
                     /gene="modB"
                     /locus_tag="JQR05_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY5324761.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3222..3995)
                     /gene="modA"
                     /locus_tag="JQR05_002618"
     CDS             complement(3222..3995)
                     /gene="modA"
                     /locus_tag="JQR05_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5324762.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4162..4311)
                     /locus_tag="JQR05_002619"
     CDS             complement(4162..4311)
                     /locus_tag="JQR05_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY5324763.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4440..5228
                     /gene="modE"
                     /locus_tag="JQR05_002620"
     CDS             4440..5228
                     /gene="modE"
                     /locus_tag="JQR05_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY5324764.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5296..6768
                     /gene="modF"
                     /locus_tag="JQR05_002621"
     CDS             5296..6768
                     /gene="modF"
                     /locus_tag="JQR05_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY5324765.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7029..8045
                     /gene="galE"
                     /locus_tag="JQR05_002622"
     CDS             7029..8045
                     /gene="galE"
                     /locus_tag="JQR05_002622"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY5324766.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8055..9101
                     /gene="galT"
                     /locus_tag="JQR05_002623"
     CDS             8055..9101
                     /gene="galT"
                     /locus_tag="JQR05_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY5324767.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9105..10253
                     /gene="galK"
                     /locus_tag="JQR05_002624"
     CDS             9105..10253
                     /gene="galK"
                     /locus_tag="JQR05_002624"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY5324768.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGNKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10247..11287
                     /gene="galM"
                     /locus_tag="JQR05_002625"
     CDS             10247..11287
                     /gene="galM"
                     /locus_tag="JQR05_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY5324769.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLSPGEE
                     YSSLTEYQFIAE"
     gene            11490..12242
                     /gene="gpmA"
                     /locus_tag="JQR05_002626"
     CDS             11490..12242
                     /gene="gpmA"
                     /locus_tag="JQR05_002626"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY5324770.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12409..13461)
                     /gene="aroG"
                     /locus_tag="JQR05_002627"
     CDS             complement(12409..13461)
                     /gene="aroG"
                     /locus_tag="JQR05_002627"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY5324771.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13777..14157
                     /locus_tag="JQR05_002628"
     CDS             13777..14157
                     /locus_tag="JQR05_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324772.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14271..15206
                     /gene="zitB"
                     /locus_tag="JQR05_002629"
     CDS             14271..15206
                     /gene="zitB"
                     /locus_tag="JQR05_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY5324773.1"
                     /translation="MAHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGGSGHSHHHH"
     gene            complement(15203..15922)
                     /locus_tag="JQR05_002630"
     CDS             complement(15203..15922)
                     /locus_tag="JQR05_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY5324774.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15960..17003)
                     /gene="nadA"
                     /locus_tag="JQR05_002631"
     CDS             complement(15960..17003)
                     /gene="nadA"
                     /locus_tag="JQR05_002631"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY5324775.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFVAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRFIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4374 a         4498 c         4288 g         4054 t
ORIGIN      
        1 cgcgaaggag atctggcggc tatgagatgg gaggatataa gagatggcag gctatacgtg
       61 gaacaacaaa aaacaggggc aaaaattcgc atttctctcc ccaccacgat ttccagattg
      121 aatttaacac ttgccgatgt actggataac ttgaaaaaaa taaacggtaa aaacgaaaag
      181 ttactcggag ggaagacagc caggacaatc gcagcacagt tcaggattgc ccgagacaca
      241 tccggattga aatgggaagg tgacccaccg ccatttcatg aaatccgaag tttatccggt
      301 cgcctgcact cagcagaaaa aggcagtgat ttcactcagg cgctccttgg acatcgttca
      361 tccagcatga cggataaata ccgtgacgga cgagggaggg aatggaaaga catttagaat
      421 taatttcgaa tgatttcgaa atgccacttg taattaattg attataaagg attaattcct
      481 aaagagcatt tgcttcatga atgctccttt gcatttagca actgactgta acagctaaaa
      541 ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg atagtggtat
      601 cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg ccatgaccac
      661 acgcgtgatt gctctcgact tagacggcac cttattgacc ctgaaaaaga ccttgcttcc
      721 atcatcgata gaagccctgg cccgcgctcg cgaagcaggc tatcaattaa tcatcgtcac
      781 aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg atacacctgc
      841 tatttgctgt aatggcacct atttgtatga ttatcatgca aaaaccgtgc tggaagcgga
      901 cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac accacattca
      961 cggtctgatg tatgtcgatg atgcaatggt ctatgagcac ccgaccgggc atgtcattcg
     1021 cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca cacaagtcgc
     1081 ttctctggct gaaacggcgc aacaagttaa cgccgtatgg aagttcgccc tgacgcacga
     1141 tgacctgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg gactggagtg
     1201 tgaatggtcc tggcacgatc aggttgatat tgcacacggc ggcaacagca aaggtaaacg
     1261 tttgacgaaa tgggttgagg cgcaaggttg gtcgatggaa aacgtcgtgg cattcggcga
     1321 taactttaat gatatcagta tgctggaagc cgctggtaca ggcgtggcga tgggcaacgc
     1381 cgatgacgcg gtaaaagcgc gcgccaacat tgtgattggt gataacacca ccgacagcat
     1441 tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca cttttaattt
     1501 gcgcgtacag ccacaggcca ggtttgatcg ccagttcatc cctggcccac gggctgatac
     1561 gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctggccg ttgtcgtcat
     1621 aactattaac aacttttgcc cgcaatacgt tacgaatgct ggtttgctgc ggcggttgta
     1681 aaaccagaga aacatcggaa gcctgaatgc ggatacgtag cgcagcttgc agcggttcgc
     1741 ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcggtcatc gcgtaatgcg
     1801 gatgatgctc aagcaccgtc actttcagaa tgctactttg ttgctctttc ggcagccacg
     1861 gattcatcac gctactgccc cacacttcct ccagcgcgcc aaaggctttc acctgaccgt
     1921 tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa tggctgacat
     1981 acaacatcgg aatgttgatt tcccgtgtca gccgttgcag ataaggcaac agttcgcgtt
     2041 tacgcggaat atccagtgac gccagcggtt catccagcaa cagcaattcc ggtgctgtca
     2101 gcaaagcccg accaatcgcc acgcgctgtt tttcgcctcc ggacaggctg cctggtaaac
     2161 ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga tcgaccatac
     2221 ttttcgacat gccgtagcgc agattgccac gcactttgta atgcgggaac agccgcgcat
     2281 cctgaaaaac atagccaacg cgacgctttt ccggcgtcag gcagatacct ttttcggcat
     2341 catttagtac ccgcccattg aggacaatcc gccctttttg cgggcgcgtc agtccactga
     2401 tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca gtgatgccat
     2461 tggcgggcag cgtttcatta atggtcaggc aatggttgcc caacgtctgg gaaaaattca
     2521 gctccagcat gattagcgcc ccgcccgttc acggctgatt ctggccagcc attctgaaat
     2581 caacagggag atcatcgcca gcgcaataga aataatgcac agtctcgccg cgccactttc
     2641 cccgccgggg gtctggatca gggtatacat ggcagaagga atggttcgcg tttcgctagg
     2701 aatgttcgac acaaaggtga tggttgcacc aaactcaccg agagaacgag caaaagccag
     2761 taccgtacca acaataattc ccggtaaggt cagcggtaac gtgatagtaa agaaaacgcg
     2821 ccagcgcccg gcacctagtg ttcttgcggc ctgttccagt ttgacatcaa ccccttccag
     2881 cgccagacga attgcccgca ccatcagcgg aaacgacatg acggcggcag cgagaaccgc
     2941 gccgcgccag ctaaaggcga aggtaatacc aaaccagtca tacagacgtt caccgataaa
     3001 tccgcgccgt cccatcgaaa ctaataataa gtaaccgacg accacgggcg gtaacaccag
     3061 cggtagatgc agtacgctgt cgagcagagc tttgcccgga aacgtgcaac gcaccagtaa
     3121 ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag aaactttcag
     3181 gcttaataaa actgcctgcc attctggatc ggtcagtatc attacttggt tgtaaatccg
     3241 taacgtttaa agatttccgc tgcctgcggt cccttcagat aatcataaaa tgctttcact
     3301 gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt atgtgaatct
     3361 tccgggaagg tggcaaccac ttttacccct ttgctggcaa ctgcgtcaga accgtagaca
     3421 atgcccagag gcgcttcgtt acgttcgacc agcgccagcg ccccacgaac atcttccgct
     3481 ggggccagtt tcggagagag cgtatcccat gcgcccagtt tttgcagtgc ttcttttgca
     3541 taaatgccag cgggaacatg ttccggatcg ccaaccgcca ggcgaccgcc attcagcagc
     3601 gaagtccagt tagttttgct gtcgatggtg aaatctttct gctcgctggc tttcggtgct
     3661 acaacgacca ggctattgcc gagcagtgtc tgacgcgtag cagtatcgat cgctttttta
     3721 tcaaccgcat aatccatcca tttctgatcg gcagaaataa acagatccgc aggggcaccc
     3781 gcttcaatct gacgggcgag agtagatgac gaagcgaaag aagaaaccac atccacgcct
     3841 ttctcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga tgcggcggcg
     3901 aacaccgtga ttttcccttc atctgccagt gcattgccag caactgcgaa agagagtgcc
     3961 gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat gtaacgttat
     4021 gtaggcgaca atataacgac taactcggaa ttttcccagc agttattgct aacctttgtg
     4081 aggtagataa gaaaaaatat cggcaggaaa agcaggaagt tgagaaaaag aaaatgcccg
     4141 actaagcggg cattcaggga atcaatgatt ttgtccgggc tggtcttttt taccaacacc
     4201 agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga tggccatcac
     4261 gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca tggtcaactc
     4321 cagatatagt catgagacta ttctaaccgc taagcacaga aaagcactcc ccttttgtgc
     4381 ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag gacattgtta
     4441 tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc gacccgcgcc
     4501 gcatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga gcgaaagatg
     4561 ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag ttaagtgagc
     4621 atattctggt cgagcgcgca acaggcggta aaggtggcgg aggcgcagta ctgacccgct
     4681 atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa aaagcctttg
     4741 atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg atctcacgtt
     4801 tttcactgca aaccagcgcc cgtaaccagt ggttcggtac catcaccgcc cgcgatcatg
     4861 atgacgttca acagcatgtt gatgtcttac tggctgacgg aaaaacacgc ctgaaagtcg
     4921 caattaccgc acaaagcggc gcacgtctgg ggctggatga aggcaaagaa gtgttgatat
     4981 tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcacaa aacgccgaca
     5041 accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc gaagtattaa
     5101 tggcgctacc cgacgggcaa acactgtgcg ccacagtgcc ggtaaatgaa gcgacttctc
     5161 ttcagcaagg gcagaatgtc acggcctact ttaatgccga cagcgtgatt atcgccacgc
     5221 tgtgctaagc gtgttgacaa tttgttatga aacacgtatc cctgtcagta atcgctgcac
     5281 aaaatgggat ataaaatgtc atcgttgcaa attttgcaag gcacgtttcg tcttagcgac
     5341 acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg ggcgtttgtc
     5401 ggttcgaatg gaagtggaaa gtcggcgctg gcccgcgcgc tggcggggga acttccgctt
     5461 ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt cgagcaattg
     5521 caaaagctcg tcagcgacga atggcaacgg aataacaccg atatgctcgg ccctagcgaa
     5581 gatgacaccg gacgcactac ggctgagatt attcaggatg aagtaaagga tgcaccgcgt
     5641 tgcatgcaac tggcgcagca gttcggtatt accgccctcc tcgaccgacg ctttaaatac
     5701 ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc ggagcctgac
     5761 ttgttgattc ttgatgagcc gttcgatggc ctggatgttg cctcacgtca gcagctggct
     5821 gagcgactcg cctcgttaca tcagtccggt attactttgg tactggtgct caatcgcttc
     5881 gatgagatcc cggaatttgt ccagtttgct ggcgtgctgg cggattgtac gttagcggaa
     5941 actggcgcta aagaggaact gctccagcaa gcactcgtcg cgcaacttgc gcatagtgaa
     6001 cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca cgccttaccc
     6061 gccaacgaat cgcgcattgt gctgaacaat ggcgtggttt cttataatga tcgccccatt
     6121 cttaataacc ttagctggca ggtgaatcca ggcgaacact ggcaaattgt cgggccaaat
     6181 ggcgcgggaa aatcgacgtt attaagcctg attactggcg atcatccgca aggttacagc
     6241 aacgatttga cgcttttcgg ccgacgtcgc ggcagcagcg aaaccatctg ggacatcaaa
     6301 aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag cactaccgtg
     6361 cgtaatgtga ttctttctgg ctattttgat tcgattggca tttatcaggc cgtttcggat
     6421 cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa acacacggct
     6481 gacgctccgt tccatagtct ttcctgggga cagcagcgtc tggcgctgat cgtccgcgca
     6541 ctggtgaaac atccgacgtt gcttattctc gatgaaccac tacaggggct tgatccgctc
     6601 aatcgccaac ttatccgccg ttttgttgat gtgctgatta gcgaaggtga aacgcaattg
     6661 ttgtttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg ccttgagttc
     6721 gtgccggacg gtggactcta tcgctatgtg ctgacaaaaa tatattgagt cggtagtgct
     6781 gaccttgccg gaggcggcct aagcgccctc tccggccaac ggttcgacgc atgcaggcat
     6841 gaaaccgctt cttttttcag ataaaaagcg caatcattca taaaccctct gttttataat
     6901 cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac taatttattc
     6961 catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata agcctaatgg
     7021 agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt catacctgcg
     7081 tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt aacagtaagc
     7141 gcagcgtact gcctgttatc gagcgtttag gcggcaaaca tccaacgttt gttgaaggcg
     7201 atattcgtaa cgaagcgttg atgaccgaga tcctgcacga tcacgctatc gacaccgtga
     7261 tccacttcgc cgggctgaaa gccgtcgccg aatcggtaca aaaaccgctg gaatattacg
     7321 acaacaatgt caacggcact ctgcgcctga ttagcgccat gcgcgccgct aacgtcaaaa
     7381 actttatttt tagctcctcc gccaccgttt atggcgatca gccccaaatt ccatacgttg
     7441 aaagcttccc gaccggcaca ccgcaaagcc cttatggcaa aagcaaattg atggtggaac
     7501 agatcctcac cgacctgcaa aaagcccagc cggactggag cattgccctg ctgcgctact
     7561 tcaacccggt tggcgcgcat ccgtcgggcg atatgggcga agatccgcaa ggcattccga
     7621 ataacctgat gccatacatc gcccaggttg ctgtaggccg tcgcgactcg ctggcaattt
     7681 ttggtaacga ttatccgacc gaagatggta ctggcgtacg cgattacatc cacgtaatgg
     7741 atctggcgga cggtcacgtc gtggcgatgg aaaaactggc gaacaagcca ggcgtacaca
     7801 tctacaacct cggtgctggc gtaggcagca gcgtgctgga cgtggttaat gccttcagca
     7861 aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg tcgcgaaggc gaccttccgg
     7921 cctactgggc ggacgccagc aaagccgacc gtgaactgaa ctggcgcgta acgcgcacac
     7981 tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag ggatatcccg
     8041 attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc cgctacaacc
     8101 cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg
     8161 cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc ttcctctgcg
     8221 caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact tacgttttca
     8281 ctaatgactt tgcggccttg atgtctgaca cgccagatgc gccagaaagc aacgatcctt
     8341 tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca ccggatcaca
     8401 gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa acctggcagg
     8461 agcaaaccgc agagctggga aaaacgtacc cgtgggtgca ggtctttgaa aacaaaggtg
     8521 cggcgatggg ctgctctaac ccgcatccgc acggacagat ttgggcaaat agcttcctgc
     8581 ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata ttttgccgaa cagaaatcac
     8641 caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc gttgtcgaaa
     8701 ccgaacactg gttagccgtt gtgccttact gggctgcctg gccgttcgaa acgctactgc
     8761 tgcccaaagc ccacgttttg cggatcaccg atttgaccga cgcccagcgc agcgatttgg
     8821 cgctggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc tccttcccct
     8881 actctatggg ctggcacggc gcaccattta atggcgaaga gaatcaacac tggcagctgc
     8941 acgcgcactt ttatccgcct ctgctgcgct ccgccaccgt acgtaaattt atggttggtt
     9001 atgaaatgct ggcagagacc cagcgagacc tgaccgcaga acaggcagca gagcgtttgc
     9061 gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa
     9121 aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc
     9181 ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct
     9241 gcgcgattga ttatcaaacc gtgatcagct gtgcaccacg cgatgaccgt aaagttcgcg
     9301 tgatggcagc cgattatgaa aatcagctcg acgagttttc cctcaatgcg cccattgtcg
     9361 cgcatgaaaa ctatcaatgg gcgaactacg ttcgtggcgt ggtgaaacat ctgcaactgc
     9421 gtaacaacag cttcggcggt gtggacatgg tgatcagcgg caatgtgccg cagggtgccg
     9481 ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc
     9541 atctgccgct ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg
     9601 ttggctgtaa ctgcgggatc atggatcagc taatttccgc actcggcaag aaagatcatg
     9661 ccttgctgat tgactgccgc tcactgggga ctaaagcagt ttccatgccg aaaggtgtgg
     9721 ctgtcgtcat catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc
     9781 gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca gccagctctg cgcgatgtca
     9841 ccattgaaga gttcaacgct gttgcacatg agctggaccc aatcgtggcg aaacgcgtgc
     9901 gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg
     9961 acctgaaacg tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg
    10021 aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcaaca
    10081 aaggtggcgt acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg
    10141 aagagctggt gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta
    10201 ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac
    10261 tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta acaacgcagg
    10321 gatggtagtc acgctgatgg actggggtgc gactttactt tccgcccgta ttccgctttc
    10381 cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagt ccggaatgct atcaggatca
    10441 ggctgcgttt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca atagtcgtta
    10501 tacctttgac ggtgaaaccg tgacgctttc gccaagccag ggcgttaacc agctgcacgg
    10561 cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaacg atcgtcaggt
    10621 gctgtttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc tcggcgcgac
    10681 ggtgcaatat cgtctgaccg acgataaccg tatctccatt acttatcgcg ccacagttga
    10741 taaaccttgc ccggtgaata tgactaatca cgtctatttc aatcttgacg gcgagcagtc
    10801 tgacgtgcgc aatcacaagt tgcagattct ggcggacgaa tatcttccgg ttgatgaagg
    10861 cggcattccg cacgacggct tgaaatctgt cgccggaacg tcttttgatt tccgcagcgc
    10921 caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga aaggttacga
    10981 tcacgcattc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc atgtctggtc
    11041 agcagatgaa aaattgcagc tgaaggtcta caccaccgct ccggctcttc aattctactc
    11101 cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa ccttacgccg actggcaagg
    11161 gctggcgttg gaaagcgagt ttctgccgga cagcccgaac caccctgaat ggccgcaacc
    11221 ggactgcttc ctgagtcctg gcgaagagta ttccagcctg acggaatatc agtttattgc
    11281 tgagtaatgt taagccctcg tcatgagggc tttatcttca tattgttcaa atcaccagca
    11341 aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgcc actattttcg
    11401 ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga gaattattat
    11461 cattaaaaga tgatttgagg agtaagtata tggctgtaac taagctggtt ctggttcgtc
    11521 atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac gtggatctgt
    11581 ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag gaaggttaca
    11641 gctttgactt tgcttacact tctgtgctga aacgcgctat ccataccctg tggaatgtgc
    11701 tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg aacgaacgtc
    11761 actacggtgc gttgcagggt ctgaacaaag cggaaactgc tgaaaagtat ggcgacgagc
    11821 aggtgaaaca gtggcgtcgt ggttttgcag tgactccgcc ggaactgact aaagatgatg
    11881 agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagaa ttgccgctga
    11941 cggaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa accattctgc
    12001 cgcgtatgaa gagcggtgag cgcgtaatca tcgccgcaca cggtaactcc ctgcgtgcgc
    12061 tggtgaaata tcttgataac atgagcgaag aagagattct tgagctgaat atcccgactg
    12121 gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc tattatctgg
    12181 gtaatgctga cgagatcgcg gcgaaagcag cagccgttgc gaaccagggt aaagcgaagt
    12241 aaacgtcatt cgtttaaaaa atgagaaagc cgactgcaag tgagtcggct tttttgtttg
    12301 tcgttgctaa caatggagca cattgcctga tgcgacgctg cgcgtcttat caggcctgtg
    12361 gtgattcatc ggatacgcca ctctgacggc gcatccgaca attaaacctt acccgcgacg
    12421 cgcttttact gcattcgcca gttgacgtaa cagagcatcg gtatcttccc agccaatgca
    12481 ggcatcggtg atgctcttac cgtaggccag cggctccccg ctatcgaggc tctgattgcc
    12541 ttccaccaga tggctttcca ccatcacgcc aataatggcc ttttcgccac cggcaatctg
    12601 ctggcaaacg tcagcacaaa catccatctg ctttttgaat tgtttggacg aattagcatg
    12661 gctgaaatcg atcatcacct gagctggcag acctgctttg ttcagccctt ctttcacttc
    12721 agcaacgtgc ttcgcgctgt agttaggctc tttaccgccg cgcagaatga tatggcaatc
    12781 gccgttacca ctggtattca caatcgccga atgcccccat ttagttacgg acaggaagca
    12841 gtgcggcgca ccggcggcat taatggcatc gatagccact ttaatcgtac cgtctgtgcc
    12901 atttttaaaa cctaccggac aagaaagacc agacgccagt tcgcggtgca cctgcgattc
    12961 cgtggtacgt gcaccaattg cgccccagct catcaggtca gcgagatact gaggagtgat
    13021 catatcgaga aactcacccg ccgctggcag accgctgtcg ttaatatcaa gcagcaattt
    13081 acgggctata cgcagaccgt cgttgatctg gaagctgtta tccatatgcg gatcgttaat
    13141 caaccctttc cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca ttacgatttc
    13201 cagctcatct ttcagctctt cacgcagcgc cagcaagcga gtggcatact ctttagccgc
    13261 gacaggatca tgaattgagc acgggccaat caccaccaac aggcgatcat cattaccttt
    13321 caggatctta tggatcgctt ttcgggcatg ggcaaccgta ttcgcggcat tttcagttgc
    13381 ggggaatttt tccagcaatg cgacaggagg aagtaactct ttgatttctt tgatgcgtaa
    13441 atcgtcgttc tgataattca tgtctgttcc agtgttgtca tacttatctt agtgaatgca
    13501 atacttccaa tctatatctt ccgtcagaat gtgtaaacgg ggttttacac tatgaacgga
    13561 ttaatcctgg aattagcaag aaaaacgcca gattgtcgcg aaaaacgaga tctatcctac
    13621 aatttctaac tgtaactcct ttgtttgtta attatttcaa gattctctgc tgcgtttcat
    13681 aaactggctg aaatcttaaa ccaatgcctt atattcacct gcaaatgcac tgttggaaga
    13741 ggttatccga cataacgacc ataacaggag catcctatga aaatgacaaa actggccaca
    13801 ttatttctga ctgccactct aagccttgcc agcggtgccg cactggctgc cgatagcgga
    13861 gcgcaaacta ataacggcca ggcaaacgcc gcagctgatg cgggccaggt agcccctgac
    13921 gcccgtgaaa atgtcgcgcc aaataacgtc gacaataacg gggtaaatac cggttctggc
    13981 ggcacaatgc tgcattcgga tggttcttca atgaacaatg acggaatgac caaagatgaa
    14041 gagcacaaga acaccatgtg caaagatggt cgatgtccgg atatcaataa aaaggtacaa
    14101 accggtgatg gcatcaataa tgatgtcgat accaaaaccg acggtaccac gcagtaaccc
    14161 tgcaaaatag ccaataatgg gagagcggag tctctccctt ttaatttttc acttctaaaa
    14221 gcagtatgat gtcataaagt taagtgagaa taattaaggg atgactcgtc atggcgcact
    14281 cacacacgtc ctcacacctg ccagaagata ataatgctcg tcgcttgttg tatgctttcg
    14341 gcgtgactgc cgggtttatg ctggtagaag tcgttggtgg ttttctttct ggttctctgg
    14401 cattgctggc cgatgcgggt catatgttga ccgatactgc cgccctgctt ttcgccctac
    14461 tcgccgtgca attttcacgt cgtcctccca ccattcgcca cactttcggt tggctaagac
    14521 tgaccacgct cgcggcgttt gtgaatgcca tcgccctcgt ggtaattacc attttgattg
    14581 tctgggaggc gatagaacgc ttccgcacgc cgcgtccggt cgagggaggc atgatgatgg
    14641 caattgccgt ggctgggttg ctggcaaata tactttcttt ctggttactt catcacggca
    14701 gcgaagagaa aaacctcaac gtacgagcag cggcactgca tgtgctcggg gacctgctgg
    14761 gttcggttgg ggctattatt gccgcgttga ttattatctg gactggctgg acacctgctg
    14821 accccattct ctcgatactg gtgtcgcttc tggttctgcg tagcgcgtgg cgactgttga
    14881 aagatagcgt gaatgaatta cttgaaggtg caccggtatc gctggatatc gctgaactga
    14941 agcgtcgtat gtgccgggaa atcccggaag tccgcaatgt gcaccatgta catgtatgga
    15001 tggtaggcga gaagccggtg atgacgttgc atgtgcaggt gatcccgcca cacgatcacg
    15061 atgccttgtt ggatcagatc caacactacc tgatggatca ctatcagatt gagcacgcca
    15121 ccattcagat ggaatatcaa ccttgtcatg ggccagactg ccacctgaat gaaggggggt
    15181 ctggccattc acatcatcac cattaatggg acagcgcgcg tgagcctctt tcacgtgcgc
    15241 tgttgatcca catccggctg ccgttgagcg caataaaggt caggatgatg tactccagag
    15301 acatggcgta aacgccctga agtgcaaaaa taacgacgct aatcacgtta ataatcaccc
    15361 acaacagcca gttttccaca tacttacgcg tcatcagaat cattgccacg atagataaca
    15421 ccatcatgca tgaatcccag aacgggaaag cgtccggttg cagttcaggc atcacaacct
    15481 gtaatcctaa tgcttgcatg atcatgactg ccacgcgggt caaaaatgca aacaccggat
    15541 tgataaatac cgtcatcaga ccaatcgaaa caacgcaaac cgccaaccag ctgagtgcct
    15601 tcggcaatgg caaccagcga attttcaact ccgcctcgtt ctgactggtt tgtcgcgacc
    15661 acgcatacca gccgtaaata ttcgcggcaa agaaaaacac ctgtaatagc aggctggcat
    15721 acagctgaat ctgaaagaaa ataatgccaa acaaggtaac gttgatcagg ccaaagaagt
    15781 agttgctgat cttctccaga ctggcaaggc caatacacag caacccggcg atcgtgccta
    15841 ccgcttcgat ccatgagaga tcataaccgc ccgcccctat tggtatatgt actaggatat
    15901 tctgcacact aaaaaaatcc atctttcccc caaagcgtaa cgttacgcct tattattcgt
    15961 tatccacgta gtgtagccgc aaaatccagc atacgattga gcggcaccag cgccctctct
    16021 cgcagcctct catcgacatg aacctcgtga ttacttcctt cctgttctaa tgcctctgcg
    16081 atagcctgaa ggccattcat ggccatccac ggacaatgcg cgcagctgcg gcaggttgcg
    16141 ccctcacccg cggttggtgc ttccagtaac tctttatctg gcaccgcctg ctgcattttg
    16201 tagaaaatac cccgatcggt tgccacaata aacctctgat gaggcaatgc ttttgcagca
    16261 gcgatcagtt gactggtgga accgaccgca tccgccatat cgacaatagc ttgtggtgat
    16321 tctggatgca ccagtatggc agcatccggg tattcttctt gcaagcgggt taacgcctga
    16381 gtcttaaatt catcatgcac aatgcaggca ccctgccagc ataaaatgtc tccacccgtc
    16441 tgtttttgca cgtaacgccc cagatgtttg tcgggtgccc agatgatttt ttcacccaaa
    16501 ctatcaagat gatcaataag ttcgacggca atgcttgaag ttaccaccca atctgcgcgc
    16561 gcttttaccg cagcagaagt gttggcgtag acgacgacag tacgatcggg atgggcatcg
    16621 caaaatgcgt taaattcttc aacagggcag ccgagatcca gtgaacactc agcctgaagt
    16681 gtcggcatca gaattgtttt ttccggactg agaattttgg cggtttctcc cataaatctc
    16741 accccagcga ctaacaaagt agaagcggga tgctttgcaa cgaagcgcgc catttccaga
    16801 gaatcagaaa tacagccacc agtttcttct gccagttgtt gaatttcggg atcggtatag
    16861 tagtgggcaa ccatcaccgc attacgttct tttagcagac gttttatctt ctcgcggtaa
    16921 tacgcttttt catcaatgct taacggcgtc ggcttcgggg ggaaaggata aatcgccgtg
    16981 tctggatcaa acattacgct catcttacca tctcgtttta caggcttaac gttaaaaccg
    17041 acattagcgt aacattcgct gttatcggtg taatttgttt tatatgctaa acaagatagt
    17101 cgaatggtgg ggaaaagtca ctcgaattac gcctaattcc accgacgtag agttgcttgc
    17161 acagtaagct ctgacgatgt catcaggttt acaaagtgaa attgaggatg cctg
//
LOCUS       DADKXM010000083        16931 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.084, whole genome shotgun sequence.
ACCESSION   DADKXM010000083 DADKXM010000000
VERSION     DADKXM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.084"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            150..689
                     /locus_tag="JQR05_002632"
     CDS             150..689
                     /locus_tag="JQR05_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324776.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(784..2361)
                     /gene="guaA"
                     /locus_tag="JQR05_002633"
     CDS             complement(784..2361)
                     /gene="guaA"
                     /locus_tag="JQR05_002633"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY5324777.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2430..3896)
                     /gene="guaB"
                     /locus_tag="JQR05_002634"
     CDS             complement(2430..3896)
                     /gene="guaB"
                     /locus_tag="JQR05_002634"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY5324778.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4058..5428
                     /gene="xseA"
                     /locus_tag="JQR05_002635"
     CDS             4058..5428
                     /gene="xseA"
                     /locus_tag="JQR05_002635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY5324779.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQFWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5425..5640)
                     /locus_tag="JQR05_002636"
     CDS             complement(5425..5640)
                     /locus_tag="JQR05_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324780.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5710..7182)
                     /gene="der"
                     /locus_tag="JQR05_002637"
     CDS             complement(5710..7182)
                     /gene="der"
                     /locus_tag="JQR05_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY5324781.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7300..8478)
                     /gene="bamB"
                     /locus_tag="JQR05_002638"
     CDS             complement(7300..8478)
                     /gene="bamB"
                     /locus_tag="JQR05_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY5324782.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8489..9109)
                     /locus_tag="JQR05_002639"
     CDS             complement(8489..9109)
                     /locus_tag="JQR05_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY5324783.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9127..10401)
                     /gene="hisS"
                     /locus_tag="JQR05_002640"
     CDS             complement(9127..10401)
                     /gene="hisS"
                     /locus_tag="JQR05_002640"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY5324784.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKQLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10512..11630)
                     /gene="ispG"
                     /locus_tag="JQR05_002641"
                     /gene_synonym="gcpE"
     CDS             complement(10512..11630)
                     /gene="ispG"
                     /locus_tag="JQR05_002641"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY5324785.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11657..12670)
                     /gene="rodZ"
                     /locus_tag="JQR05_002642"
     CDS             complement(11657..12670)
                     /gene="rodZ"
                     /locus_tag="JQR05_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY5324786.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12955..14109)
                     /gene="trmG/rlmN"
                     /locus_tag="JQR05_002643"
     CDS             complement(12955..14109)
                     /gene="trmG/rlmN"
                     /locus_tag="JQR05_002643"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY5324787.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14259..14690)
                     /gene="ndk"
                     /locus_tag="JQR05_002644"
     CDS             complement(14259..14690)
                     /gene="ndk"
                     /locus_tag="JQR05_002644"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY5324788.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14839..16931)
                     /gene="pbpC"
                     /locus_tag="JQR05_002645"
                     /pseudo
     CDS             complement(14839..16931)
                     /gene="pbpC"
                     /locus_tag="JQR05_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
BASE COUNT         4077 a         4730 c         4215 g         3909 t
ORIGIN      
        1 aaggcaccaa agtgtatacc tccacccagg tgggtaaaga gtgccagttt acgacaggtt
       61 tagccgttgt tattaccaca acgtataacg aaacgcgtat tcagccaaat accaaatgtc
      121 ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc gccatcttag
      181 cctccttaag ctatccggct atagcctcat cattgcagga tcaactctct gctgtcgcag
      241 aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg gtcgcggagc
      301 gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa tgcccaggcc gccgcgaaca
      361 aaagagcggc aacggcagcg gcaaataaga aagctcgtca ggataaactg gacgccgaag
      421 ccactgcgga caaaaaacgc gatcaaagtt atgaagatga gctacgcagc ttagagattc
      481 agaaacaaaa actggcgctg gcgaaagaag aagcccgcgt taagcgcgaa aacgaattta
      541 tcgatcagga actgaagcac aaagctgcgc aaaccgatgt ggtgcaatct gaagctgacg
      601 caaacagaaa tatgactgaa ggcggtcgcg atctgatgaa aagcgtgggc aaagcagaag
      661 agaacaaatc ggacagctgg tttaattaat cgatgttagt aacttcaagc ctatgattct
      721 tgaagataaa aaaccctctg tagtaacgga gggttttgtt cattcatagt gcagggatca
      781 aaatcattcc cactcaatgg tagctggcgg cttgccgctg atgtcataca ccacgcggga
      841 aataccgttc acttcattga taatgcggtt ggaaacgcga ccgaggaaat cgtacggcag
      901 atgcgcccag tgtgcggtca taaagtcgat ggtttcgaca gcacgcagag agacaaccca
      961 gtcatactta cgaccatcgc ccattacgcc aacggaacgt actggcagga agacggtgaa
     1021 cgcctggctg actttgtcgt acaggtccgc tttacgcagt tcttcaatga agatggcgtc
     1081 agcacggcgc agcaggtcac agtactcttt cttcacttca cccagaacac gaacgccaag
     1141 gcctggtccc gggaacggat gacggtacag catgtcgtac ggcaggccca gctccagacc
     1201 aatcttacgc acttcgtctt tgaacagctc tttcagcggt tcaaccaggc ccatcttcat
     1261 ctctttcggc aggccgccca cgttgtggtg agatttgatg acgtgtgctt taccggttgc
     1321 agaagccgca gattcgataa cgtcagggta gatggtgccc tgcgccagcc acttcacgtc
     1381 ttccagtttc agcgcttctt catcgaatac ttcaacgaaa acgcgcccga tgattttacg
     1441 ttttgcttcc ggatcgtttt cgccagccag cgctgacagg aagcgatctt ctgccggtac
     1501 gtgaacaatg ttaagaccaa agtgatcgcc aaacatatcc agaacctgct ctgcttcgtt
     1561 gagacgcagc aggccgttgt cgacgaatac gcaagtcagg tttttaccga tagcacggtg
     1621 cagcagcatt gcggttacgg aggaatccac accaccagag aggccgagga tgactttatc
     1681 gtcgcctacc tgctcgcgga tgcgagctac agcatcgtcg ataattttcg ctggcgtcca
     1741 cagggcttca cactggcaga tatcacgcac aaaacgctcc agcatgcgca taccctggcg
     1801 ggtgtgagtc acttccgggt ggaactgtac gccatagaag cgtttttctt cgttagccat
     1861 aatggcaaac gggcagcttt cggtgctggc tacggtgacg aagtcggacg ggatagcggt
     1921 aactttatcg ccgtggctca tccagacatc gagcagcggt ttaccgtctg cggtcagcgc
     1981 atcttcgata ccgcgaacca gtgcgctgtc gtttacgact tcaacctgcg cgtagccaaa
     2041 ttcacgttcg ttagaggctt caacgtgacc gcccaactgc attgccatgg tctgcatgcc
     2101 atagcaaacg ccgaatatcg gtacgcctgc ttcaaagaca tactgcggcg cacgcggact
     2161 gttttcttca gtagtacttt ccgggccgcc ggaaagaata atgccgcttg gattgaagtc
     2221 acgaatttgt gcttctgtca catcccacgc ccacagttcg cagtaaacac ccagctcacg
     2281 cacgcggcgc gcaaccagtt gagtgtactg agaaccgaag tccagaatga ggatgcgatg
     2341 cttatgaatg ttttccgtca ttgacgctta ttccgaggca agtgaaacag ataatataaa
     2401 tcgcccgaca tgaagtcggg cgaagagaat caggagccca gacggtagtt cggggactct
     2461 ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatac ccgcaccgct gatacgtaca
     2521 aactccgctt tagtacgcag ttcgtcgata gtaccacagc cggtcagacc catacaggag
     2581 cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc gacctttata agctacgcga
     2641 ccttcgatac cttccggcac cagtttgtcg gcagcgttat cgctctggaa ataacggtca
     2701 gaggaacctt tggacatcgc gcccagggaa cccataccac ggtaagattt ataagaacgg
     2761 ccctggtaga gttcgatttc acccggagat tcttcagtac ccgccagcat ggaacctacc
     2821 atcaccgcgc ttgcgccagc ggcgatagct ttggcgatgt cgccggagaa gcgaatacca
     2881 ccatctgcga taaccggaat accggtgcct tccagggctt caactgcgtc agcaacggca
     2941 gtgatctgcg gaacacctac gccagtaacg atacgagtcg tacagataga accagggccg
     3001 ataccgactt taaccgcgct gcaaccagct tctgccaaag cgcgtgcacc tgcagctgtt
     3061 gccacgttgc cgccgatgat ttgcagatcc ggatatttag cacgggtttc acggatacgc
     3121 tgcaaaacgc cttctgagtg accgtgggag gagtcgatca gcagaacgtc aacgccagcg
     3181 gcaaccagcg cgtcaacacg ctcttcgtta cccgcacctg cgccaactgc agcaccaaca
     3241 cgcagacggc cttgctcgtc tttacaggcg ttcggtttac gttccgcttt ctggaagtct
     3301 ttcacggtga tcatgccgat caggtggaat tcgtcatcaa ccaccagcgc tttttcaacg
     3361 cgtttttcgt gcatttttgc cagcaccact tcacgggctt caccttcacg cacggtgacc
     3421 agacgctctt tcggcgtcat gtaaacgcta accggctggt tcaggtcggt aacaaaacgc
     3481 acgtcacgac cggtgataat acccaccagt tcgttttctt cggtaacgac cggatagccc
     3541 gcaaaaccgt tacgctcggt cagttctttc acttcgcgca gcgtcgtggt tggcagaaca
     3601 gtctgcggat cagtcaccac accagattcg tgttttttca cacggcgaac ttcttctgcc
     3661 tggcgttcaa tggacatgtt tttgtggata aagccgatac cgccttcctg agccagagca
     3721 atagccaggc gcgcttccgt tacggtatcc attgctgcgg aaagcatagg gatattcaga
     3781 cgaatagttt tcgtcagctg ggtgctgagg tcagcagtat tcggcagaac ggtagaatga
     3841 gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt tagcgatacg tagcatgggc
     3901 aatatctcga ccagagtggt taataaatat tgccgcggca ttatacagag cgtaaccgat
     3961 tgcatctacc cctttttgca aaaaatgctt gctatccccg aagggcgggt tactatcgac
     4021 tgaataacct gctgatttag aatttgatct cgctcacatg ttaccttctc aatcccctgc
     4081 aatttttacc gttagtcgcc tgaatcaaac ggttcgtctg ctgcttgagc atgagatggg
     4141 acagttttgg atcagcggcg aaatctctaa tttcacacaa ccggcttccg gtcactggta
     4201 ctttacactc aaagacgaca ccgcccaggt acgctgcgcg atgttccgca acagcaaccg
     4261 ccgggtcacc ttccgtccgc agcatgggca acaagtttta gttcgcgcca atattacgct
     4321 ctacgagccg cgcggcgact accagataat cgttgagagt atgcagccgg ccggtgaagg
     4381 gctgctgcaa cagaagtacg aacagctcaa agcgaagttg caggctgaag gtttgttcga
     4441 tcagcaatac aaaaaaccac ttccctcccc tgcgcattgc gttggtgtga tcacctcaaa
     4501 aaccggtgct gcgctacatg atattttgca tgtgttaaaa cgtcgcgatc cttctctacc
     4561 ggtgatcatc taccccacct ccgttcaggg cgatgacgcg ccggggcaaa ttgttcgcgc
     4621 cattgaactg gcgaatcagc gcaatgagtg cgacgtgttg atcgttgggc gcggcggcgg
     4681 ttcgctggaa gatttatgga gttttaacga cgaacgcgta gcgcgggcga tttttgccag
     4741 ccgcattccg gtcgtcagcg ccgtcgggca tgagacggat gtgaccattg ccgattttgt
     4801 tgccgatctg cgtgcgccaa cgccgtctgc cgccgctgaa gtagtgagcc gtaatcagca
     4861 agagttactg cgccaggtgc aatcggcccg tcaacggctg gagatggcga tggattatta
     4921 tctcgccaac cgtacgcgtc gctttacgca gattcatcat cgcttgcagc agcagcatcc
     4981 gcagctccgg ctggcacgcc agcaaaccat gcttgaacgc ctgcaaaaac ggatgagctt
     5041 tgcgctggaa aatcagctta agcgtgccgg gcaacagcag cagcgattaa cacagcggct
     5101 gaatcagcaa aatccacagc cgaagattca tcgcacgcaa acgcgcattc agcaactgga
     5161 atatcgttta gcagaaatcc tgcgcgcaca gcttagcgcc acgcgtgaac gtttcggtaa
     5221 tgcagtaacg cacctcgaag ccgtaagccc actgtcaacg ctggcgcgtg gatacagcgt
     5281 tactactgct actgacggca atgtactgaa aaaagtgaag caagttaaag cgggtgaaat
     5341 gctaaccaca cgtctggaag acggctggat agaaagtgaa gtaaaaaaca tccagccggt
     5401 aaaaaaatcg cgtaaaaagg ttcattaagc cagcacgaat tcaacgcgtt ttttcgatat
     5461 caagccatgc ccgtgctgac agaagtaatc tactgcaccg caggctttta acacctgcaa
     5521 cggttgatgg cagtctggac aaagagcttt catttcaata aattccccgc aggatgggca
     5581 acgcgcatgg ccgttatcct gatcaagcac atgctgacat tttggacaat gcagttccat
     5641 atgaattcct ccggtagcca ttccctctac attcatagag ggaatggcag ataaaatact
     5701 tacggataat tatttatttt tcttgatgtg cttcatcaga cgcttacgtt tacgcatctg
     5761 ggttggcgtc agggtgttac gcttgttcgc atacgggttt tccccttctt tgaactgaat
     5821 acgaatcggc gatcccatta cgtccagcga tttgcggaag tagttcatca agtagcgctt
     5881 gtaggaatca ggcaggtctt tcacctgatt accgtgaatc accacaatcg gcgggttata
     5941 accaccagcg tgggcatatt tcagcttcac acgacgaccg cgtaccagcg gcggttggtg
     6001 atcttcaaca gccatcgtca tgatgcgcgt cagcatagag gtccccacac gacgggtgga
     6061 gctgtcatac gcttcacgta ctgattcaaa caagttacca acaccactgc cgtgcaaggc
     6121 agagataaag tgcacacgag caaaatcgat aaagcccaga cggaagtcca gcgtttcttt
     6181 cacctgctct ttcacttcct ggctcaggcc atcccactta ttcaccacaa tgacaagtga
     6241 gcgcccacta ttgagaataa agcccagcag cgagagatcc tgatcggaaa taccttcgcg
     6301 cgcatcaatc actaacatca ccacgttggc gtcttcaatg gcctgcaacg ttttgattac
     6361 ggagaatttc tctacagcat cggtgatttt gccgcgttta cgtacgccag cggtgtcaat
     6421 gagcacatac tcacgtccat cgcgttccat tgggatgtag atgctgtcac gcgtcgtgcc
     6481 aggcatgtcg taaacaacaa cgcgctcttc tccaagaata cggttagtga gtgtagactt
     6541 acctacgttc ggacgaccca caatcgccag tttgatcggc agactttgcg ggtcgaagtc
     6601 gtcttcctct tcttcttcgc cgttctcttc cgcttcaaat tgcgcccagt attcagcgtc
     6661 ttcgtcgact tcctcttgcg gtgcgagatc ttccatccac ggcagcagca catgctccag
     6721 cagacttaat acgccacgac cgtgagacgc ggcgatcggg tagatttcac ctaaaccaag
     6781 cgagtagaaa tcaaccactg cctgatcggg atccagaccg tcagttttgt ttgccaccag
     6841 gaaggtcggt ttttcacggg agcgcagatg tttggcaatc gcttcatctg ccggcatcag
     6901 gcccgcgcgc gcatccacca taaacagtac gacgtccgct tcttcaatcg ccagcagcga
     6961 ctgttccgcc atgcgggttt ctacaccgtc ttctgtgcca tcaatcccgc cggtatcaat
     7021 acagataaac tcacggcctt caatttccgc acgaccgtac ttacggtcac gagtcagacc
     7081 cgggaaatcc gcaaccagcg catctcgggt gcgagttaaa cggttaaata acgtggattt
     7141 tcctacgtta gggcgcccga caagcgcgac cacaggtacc atgtttaaag cctcattttt
     7201 ataaatcatc agacaacgca cgctatattc gcgtcgttgt taaaaacagg aaaacggccc
     7261 ctgtccagga gccgttttca aagtgaacga cagagacgat taacgtgtaa tagagtacac
     7321 ggttccgtct tttgcctgga tcagcagttt gccgtcagcg gcaaccggtt cagtctggaa
     7381 accggaacta tcaacttttt gctgggcaac gaaacgacca tcttcgacgt taatccagtg
     7441 cagataacct tcactgtcac cgaccaccag gttgccatta tacagcaccg gagaagtcag
     7501 caggcgatgc agcagatcgc tttgtgccca cagcgtaacg ccgccatcaa tggtcaacgc
     7561 catcacccgg tcattttgat cgaccagata gatgcgattg ccatcgacga tgaaatcatt
     7621 caccgaaccc agttcgcgtt tccacataat ctgaccactg cgcagatcaa gcgccgtcag
     7681 gttaccatta taggccagcg cgaaaacaac gccgttaaca acgacgggag tcgtgtcaac
     7741 atcgctcaga cggtcaattt cggtagaacc ggtcgcctgg gaaatacgct gctgccaaat
     7801 catctggccc tgttccatca gcactgcgct gacgcgacca ttatcgccac ccacgacggc
     7861 cgcaccaaaa gccgttgccg gcgcagactc gccacgcaaa gagagcgaag gcatatcgag
     7921 gttaactgtc catttgacag cgccgtcagc ttcgttcagc gcttgtaact gaccgttact
     7981 ggtgtggatt aacaccagac cgtcgctgac caccgggcgc gaaagtgctt cacccgcgac
     8041 tttagtttgc catgccacag taccatcgct ggtattcagc gcgtaaacct gcgccttttc
     8101 gctgccaatg tagacatgcc caccagacac ggtcacaccg ccagaaagta atgcaggctc
     8161 tttagagaac cagccatctt tctcggccag gctgacagac cagatttctt tgccatcatc
     8221 cgcattcagc gcttttacca aaccagcgcg gtccgctgca tagacaacgt tgtccgccag
     8281 tgccggatga agattggaat agaagttgcc aatgccgcta ccaacggaag tgctccacgc
     8341 cgtggtcggc gtaaactggt tttcaacggt tggcaatggg gacatcttta ccacatcttc
     8401 ttcgctgtta aacagcgaac agccgcttaa aagggtaacg gaaagcagtc ctggcagcag
     8461 taatttacgc aattgcatcg ggtccctctc agatggacaa attattaatt ttcatctgca
     8521 tcatttcgct cagtgccgga gtaacatcgc ttttcacgcc tgcttcccat gcactacgcg
     8581 caccttgctt atcacctttg ctcagcaatg cttcaccacg caggtcggca acaatggcag
     8641 cccacccttc acctttgatg gtatcaaggg ttttcagcgc ggcatcagcc tgcttgagct
     8701 gtacctgaac gcgagcaaga cgcagattta tcacggcttt gagattttca tcgctcgtgt
     8761 ctgccagccc ctgttgtaac tgggcggcag ctttctctag ttcatttttg tcaacaaatt
     8821 gctgcgcaag ttccaaagaa gccagcgcac cataagtatt tttattttca gcagcaaatt
     8881 tttccgccgc cgggatgcta tccggtttgc cttcgctcac tgcggtaacc gcattttgat
     8941 aggcaagaga agcggagcgt gcagaatcaa cctgatggct gttccagtag cgccagccaa
     9001 tcagtgcgcc aacgcccaaa atcaccccaa cagccagtgc tttgccattt tcagcaaaaa
     9061 agcgtttaac cgcttctacc tggtcgtttt cgttctcgta aatttccacg ctgtccttct
     9121 ccttccttaa cccagtaacg tgcgcaaatg cgcggctacg ctatcctgcg caactgccgt
     9181 ttgctcacca gagcgcaaat ccttcactac tgctgtgccg ttagccactt cagactcacc
     9241 cagcaccaca gcaacgcggg caccccattt atcagcacgg gcaaactgtt tcttaaagtt
     9301 gccgccgccg tggttggtca tcaatttcac gcccagtaat tcatcacgca gacgctcagc
     9361 taatgccata gccgcagatt gtgtatcagc acctgaagcc accaggtata tatcgacaac
     9421 aggatcggct ttaaattccg gattaacggc ctgtactaac aatacaagac gttcgaggcc
     9481 catcgcaaaa ccgacagccg gtgttgcacg accgcccagt tgttccacaa gaccgtcata
     9541 acgaccgcct gcacacacgg tgccctggga gccgagactg ttagtcaccc actcgaaaac
     9601 ggtacggtta tagtaatcca gaccacgcac cagacgctgg tttacggtgt aagcgatccc
     9661 cgcgctctcc agctgtttgc acagaccggc aaaatgctca cgagattcct cgtccagata
     9721 atcacctaat gccggagcgt cgttgagaag cgcctgcact tccggatttt ttgaatccag
     9781 cacgcgcagc gggttagtgt acatgcggcg tttgcagtct tcgtccagct tttctttatg
     9841 ctgctcaagg aatgccacca gcgcatcgcg gtaattggcg cgtgcttcca gcgaaccgat
     9901 agaattcagc tcaagagtta cgtgttcgaa gatacccagc gcacgccacc agcgggcagt
     9961 gagcataatc agttcagcgt cgatatccgg accttgcaga ccgaaaactt cgcagcccaa
    10021 ctgatggaac tgacgataac gccctttctg cggacgctcg tgacggaaca tcggcccgat
    10081 ataccacaga cgctgttcct gattgtacag aagaccatgc tcgatgccgg cgcgtacaca
    10141 gcccgccgtc ccttcagggc gcagagtcag gctgtcgcca ttgcgatcct caaaggtgta
    10201 catctctttt tcaaccacgt cggtgacttc accaatcgca cgtttgaata gcggggtctg
    10261 ctctacaatc ggcaagcgga tttcactgta accgtagctg ccgagcacgt ttttcagtgt
    10321 gccttcaatg cgctgccaga tggccgtttc gccaggcagg taatcgttca tgccgcgaat
    10381 ggcttgaatg ttttttgcca cgtttattct ctttctgaat ataaaaatga accctcaacg
    10441 cttccctcaa tgtttcggga gccatgcggg ttcaatcata cacgggaagc gaggcgcttc
    10501 ccatcacgtt attatttttc aacctgctga acgtcaattc gacgcgcttc gtccagctga
    10561 ctggctttcg cacgaatgcg tgcttccagc tggtcgatca tatcgttgtt gtccagacgg
    10621 tctttgcgca cgccatcttc atagaggccg cttttcttgt tgccgccggt gacgccgagt
    10681 gtagaaacca gcgcctcacc tgggccattc accacgcagc cgataatcga aacgtccatc
    10741 ggagtgatga tatcttccag gcgttgctcc agcgcgttaa ccgtaccgat aacatcaaat
    10801 tcctgacgcg aacaggtcgg gcaggcgatg aagttaatcc ctcgcgaacg gatacgcagc
    10861 gatttcaaaa tatcgaaacc gactttgatc tcttcgaccg gatcggccgc cagcgatacg
    10921 cgcagcgtgt cgccgatgcc ttccgacagc agcagaccta aaccaatggc ggattttact
    10981 gccccgctgc gtgcaccacc ggcttcggtg atccccagat gcagcggctg atcgatctgt
    11041 tttgccagca aacgataaga ctcaacagcg aggaagacgt cagacgcttt cacgctgact
    11101 ttgaactgat cgaagttcag gcgatcgaga tgatcaacat gacgcatggc ggattccagc
    11161 aacgcctgcg gcgtcggttc gccatacttt tcttgcagat ctttttccag cgatccggcg
    11221 ttaacgccaa tacggatcgg aatgttttta tcgcgcgcac agtcaaccac catgcgaata
    11281 cgctcttcat taccgatatt accagggtta atacgcagac aatcgacgcc gtattccgct
    11341 actttcagcg caatgcgata gtcgaagtgg atgtcagcca ccagcggcac gttaacccgc
    11401 tgtttgatga gtttgaacgc ttctgccgcg tccatcgtcg gtacggagac acggacgata
    11461 tcagcgccaa cgcgttccag cgccttgatt tgattgaccg ttgcttcgac atctgtcgta
    11521 cgcgtattgg tcatggactg tacggcgatg ggagcaccat cgccaatcgg cacattccca
    11581 acgtaaatac gtgttgattt tctacgttga attggagcct ggttatgcat gaaaaatctc
    11641 ccacgttacc cgtctgttac tgcgccggtg attgttcggc attgagggtc agacgcgcaa
    11701 cctggttagt tctgataaaa cgactcagat cgacaggttt cccttgatac tggatctgta
    11761 ctgcggctgg cgcaccaatt ttcagtttat acggtgcctg gccggttaaa ttcaaattac
    11821 cgtctttacg ctgcatcccg ctaaacaatt ttttaccggt agcatcagtg acctccagcc
    11881 agcaatcggc agtaaagttc atcaccagcg cattcggatc agccgccggc gtggtcacgc
    11941 cagcctgatc ggttggcaac ggcgcagcac catctggcgt tgttgctgcc gttggtaccg
    12001 gggtcgcggc ggtatcaaca tttgcctgcg aaggcgaaac aaccgcattc tgttgcggat
    12061 caacagctgg tgctggcgca gttacggcag gtgtttgcgt gttggttgcg gtagtatcca
    12121 cagacgctgg cggcgtggac gttgtagccg ggtctgtagt tgtcgacgta tttaacggaa
    12181 cactctgccc ctgctcgcta ttactgctca gttccgccga agattgatcg gccatagtgg
    12241 tgatctcttc ctgctgagct ttgtggtctt gccaccacca ggcaccgctc aggccgataa
    12301 ccacaaacaa caccagccag gtgaaggtca tcagccagcc gtcgcgtttt ttgcggcgtt
    12361 taccgaggga aaaactctgc attggcgcaa cttttgcagc ccgaagtgga gcctgctttt
    12421 ccagccctgg cagcagttct tcttctggaa tatgtaccag acgcgcataa gagcggatat
    12481 atccgcgcag gaatgttgaa gcaagatcgg cgggtgcctt atcttcttca atgtcgcgta
    12541 ccgtggaaac cttcaggcaa agtcgctcgg caacggcctg ctgactaagt cctagttgtt
    12601 cgcgagcatt acgcaggcga gcgccggtag taagtgcttc attttggtcg tgcgtggctt
    12661 cagtattcat tcgctgcagg tacgtttaaa tgagaattga gatgccggtg aaccatcatg
    12721 ctcacccaca ccgcgaaaca tccgttaagt taaccgttat catacagtat aagaccgtct
    12781 gtgtgctcgt gacaaagccc acattcactg aggctaactt actgttgcat cgttacatac
    12841 tgccttaaag tcagcaaaaa cgcaccgtta atattgacca gacaattgca acttaatact
    12901 tcattcataa aagattacgc cacggtacat aaagtaaccg tggcgtaatg gctatcagac
    12961 cgctttaata tcgatggctt caccctgcat ccgtttacgc agagtacgtt tcgtacggtc
    13021 gataacatcg cccgccaact gaccacaggc agcatcgata tcatcaccac gcgttttacg
    13081 cacaatagtg gtgaaaccgt agctcatcag cacttttgag aaacggtcga tacggctgtt
    13141 cgaactgcgt ccatacggcg cgcccgggaa cgggttccac gggatcaggt tgatcttaca
    13201 cggcgtatct ttcagcagtt ccgccagttg gtgcgcgtgt tcagtgccgt cgttaacgtg
    13261 gtcaagcatc acgtattcaa tagtgactcg gccctgattg gcgttggatt tctccagata
    13321 acggcgcacc gcagcaagga acgtttcgat attgtacttt ttgttgatcg gcacaatttc
    13381 gtcacgaatt tcgtcgttcg gcgcgtgcag ggaaattgcc agtgcaacgt cgatcatatc
    13441 gcccagttta tccagcgccg gaactacacc ggaagtggaa agcgtcacgc gacgtttaga
    13501 caggccaaaa ccgaaatcat caagcatgat ttccatcgcc ggaacgacgt tgttcaggtt
    13561 gagcagcggc tcgcccatgc ccatcatcac gacgttagtg attgggcgct gaccggtgac
    13621 ttttgctgcg ccgacgattt tcgccgcacg ccacacctgg ccaataattt ccgatacccg
    13681 caggttgcgg ttaaagccct gctgggcggt ggaacagaat ttacactcca gcgcacaccc
    13741 cacctgcgaa gagacgcaga gcgtggcacg atcgtcttcc gggatataca ccgtttcgac
    13801 gcgctgatcg ccaacggcga tcgcccattt aatggtgccg tcggatgaac gctgttcttc
    13861 aaccacttcc ggtgcgcgga tttccgccac ctctttcagt ttgccgcgca acactttgtt
    13921 gatgtcggtc atctcatcaa agttgtcgca gcaatagtga tacatccact tcatcacctg
    13981 atcggcgcgg aaggtttttt cacctaaatc tttaaaaaac tcccgcatct gctgacggtt
    14041 gagatccagc aggttgattt ttccatcttt cgtggtgacg ttttcaggtg tgactaattg
    14101 ttcagacata tgctattccg gcctcgttat tacacgttat ggcccctgga gggttgaaaa
    14161 aagaaacgcc ccggtgagcg gctgctcgtc cgggggcgct gcattgtaca aattctggcg
    14221 cacggatgcc acgtttgcac gcggcattta cgaaattatt aacgggtgcg cgggcacact
    14281 tcgccttcgc cgaagaaata agcgatttcg cgagcggcag attcgacgga atcagaaccg
    14341 tgggtaccgt tttcggtcag gctgtcagcg taatcagcgc gcagagtacc agccagtgcg
    14401 tttgccggat tggtcgcgcc cagcagatcg cggtgacgct gaacggcgtt ttcaccttcc
    14461 agcacggaaa ccacgatcgg gccagaggtc atgaattcaa ccagaccatc aaagaacggt
    14521 tttccatcgt gttcagcata aaagccacgt gcctgttcaa cggtcaggtg cagcattttg
    14581 gtgccgacaa ttttgaaccc tgcagcttca aagcgcgcaa agatattacc aatgacgttt
    14641 tttgctaccg cgttcggttt gatgatggaa aaagtacgtt caatagccat ttttacctct
    14701 gtaaattgtt ctgttgttgt ctgtaccagc gtacgagatg gcgcggatta taatgagcaa
    14761 cagggccgtt gactattgat gaaggtaaca tttttttaaa ataaaatgag ttttagcaac
    14821 aatcagggac aaaacagact attgcatgac aaatttcact gtcgcgattt gccccacatc
    14881 atccatcacc agcaattgat aatcaccttt atccgtcaaa tgcagggtca cgttgcgccc
    14941 gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt tcacctgccc ctccacttga
    15001 ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatcg cgccatcgcg
    15061 aacgcctgtc agctgcagag ggagctgggc atcgtgaccg tatggcgggc aaattgtcga
    15121 ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc agccagggtt ccagcggtag
    15181 cggccagaca tttaccattt cttgcttcgc ctgcgggcag tcagcggcga cgcgctgccc
    15241 tttgtcatcc agccagatgg ggaagcgaat gccattaatc ccttcctgct ctgacagtaa
    15301 cagcgttggc ggctggctgc catccagcag ccaggttgcc aggcggcggc gacagttacc
    15361 gtcaccttcc ggcaaagact gtccgcccgg ccagcagata acgccacgag tgactgagtt
    15421 cgggcgcggg tcttccggca gattcgcact gcgcgacagt aagatattat tgacctgatt
    15481 caacaatggt acggcactgg caaagccaaa ctgaccaaca acgggcgtgc cgtcaggtct
    15541 gccagtccag atcccaatga catagcgagc gttaacccca atcgcccagg cgtcacgata
    15601 gccatagctg gtgcccgttt tccatgccag tggggcgacg cgcggcaagg cactatccgg
    15661 caagggttgc gcttcatcag ccataatccg gcgaatgatc cacgccgccc ccgacgacat
    15721 taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg ccttgccgtg
    15781 gcgagcaaac gcggtatacg ctgccgccat atcttccagt tttgcaccag cgccgccgag
    15841 aatgagtgaa agattcggcg cagcgccgtt gggcaaatat aacggcaatc caacattgcg
    15901 taactttgcc gcaaaccgtt tcggtccata ggcttccagc acctgcacag caggtaagtt
    15961 cagcgagcgc accagcgcct cgctcatgtt gatcgggcca tgaaaaccgc tatcaaagtt
    16021 acctagtcga taatcaccgg tgcgccgggg gacgtcttgc agcagtgatg ccgggtggat
    16081 caagccttca tccagcgcca gaccataaac aaacggtttg agcaccgatc ctggcgatcg
    16141 gatcgcattg accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgatcc
    16201 cacccagccg cgaacacgca tatcggtatg attaaccacg atcatcgcca gtgagctgcg
    16261 cggtggcaac cgccctttcc agttttgcgc cagttcttcc agacgtcgtt gaagaccagc
    16321 atccagcgta gtaacgattt tgtcgctttt gcttttaccg agcatcatgc gcgaaaacag
    16381 cggtgccagt tgcggcattt gtcggggtgc cagccagatg ggttcttccc ttgactcttt
    16441 tacctgctca cgggaccaca caccttgcgc agccatccgt tcgagcactt tattacgcgt
    16501 attacgcgcg gcttcggcac gctccggcca acgatccggg cgcagtcggc tgggcgcttg
    16561 cggtaacacc gccagcattg ccgcctagga atagcttaaa ttcgcaggcg attttccgag
    16621 ataagcccaa cttgccgcac cgatcccctg caacgtaccg ccaaacggag cgcggttaag
    16681 atacaagatc agaatttcac gcttagacaa atgccactcc agttgcaacg cgcgccagag
    16741 ctggagaatt ttgccaccaa atgttttggg gtgaggatca agcagacgag caacctgcat
    16801 agtgagcgtg ctgccaccgg aaataacccg tcccgaagtg agatcttgcc atgctgcgcg
    16861 cgccaccgag aatggattaa cccccggatg cttccagaac cagcgatctt cataattgat
    16921 cagcgcttca a
//
LOCUS       DADKXM010000084        16897 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.085, whole genome shotgun sequence.
ACCESSION   DADKXM010000084 DADKXM010000000
VERSION     DADKXM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.085"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JQR05_002646"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JQR05_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5324789.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JQR05_002647"
     CDS             2219..2767
                     /locus_tag="JQR05_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY5324790.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JQR05_002648"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JQR05_002648"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY5324791.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JQR05_002649"
     CDS             complement(3664..4854)
                     /locus_tag="JQR05_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY5324792.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHALDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQR05_002650"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQR05_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY5324793.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JQR05_002651"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JQR05_002651"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY5324794.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JQR05_002652"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JQR05_002652"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY5324795.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="JQR05_002653"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="JQR05_002653"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY5324796.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDTPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JQR05_002654"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JQR05_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY5324797.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGPDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JQR05_002655"
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JQR05_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY5324798.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JQR05_002656"
     CDS             complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JQR05_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY5324799.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15295..16254)
                     /locus_tag="JQR05_002657"
     CDS             complement(15295..16254)
                     /locus_tag="JQR05_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5324800.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRTGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JQR05_002658"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JQR05_002658"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY5324801.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVL
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4217 a         4376 c         4327 g         3977 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgcgatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagccca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggcg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgat
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacctag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgcaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagagt gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatactccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gtggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatcagg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccggacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctaccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggc gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gctgccacag ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cttgtgcttt ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gcccatacgc ggcagctttc gcccgcactt gttcgatttt ctctttaaca agttccggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14821 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14881 gccagacctg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gggcggcaac agtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttcccagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15421 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 atagggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
    15781 gatatcggtg aatttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatac
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
    16141 aatactgcct ttctgatagc ctgtacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatgggcat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agatgggttt gcagatttgg cgtgaactgg ggtctgtgat
    16381 ggtaatcaat taattgtgag tcatcggcaa acacgccgtg caggatctcc tgagctttca
    16441 gtgcgcttaa aactggttta agggcataat cgaccgccag cagatgggcc acggtaccgc
    16501 ccgtcgccag cggtagcaac actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tctaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaag
//
LOCUS       DADKXM010000085        16412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.086, whole genome shotgun sequence.
ACCESSION   DADKXM010000085 DADKXM010000000
VERSION     DADKXM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.086"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1075)
                     /locus_tag="JQR05_002659"
     CDS             complement(<1..1075)
                     /locus_tag="JQR05_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY5324802.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLW"
     gene            complement(1274..1358)
                     /locus_tag="JQR05_002660"
     tRNA            complement(1274..1358)
                     /locus_tag="JQR05_002660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Leu,seq:caa)
     gene            1554..2573
                     /gene="ahr"
                     /locus_tag="JQR05_002661"
     CDS             1554..2573
                     /gene="ahr"
                     /locus_tag="JQR05_002661"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY5324803.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2702..4204
                     /locus_tag="JQR05_002662"
     CDS             2702..4204
                     /locus_tag="JQR05_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY5324804.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4323..5405)
                     /gene="lptG"
                     /locus_tag="JQR05_002663"
     CDS             complement(4323..5405)
                     /gene="lptG"
                     /locus_tag="JQR05_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY5324805.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5405..6505)
                     /gene="lptF"
                     /locus_tag="JQR05_002664"
     CDS             complement(5405..6505)
                     /gene="lptF"
                     /locus_tag="JQR05_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY5324806.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6772..8283
                     /gene="pepA"
                     /locus_tag="JQR05_002665"
     CDS             6772..8283
                     /gene="pepA"
                     /locus_tag="JQR05_002665"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY5324807.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8417..8860
                     /gene="holC"
                     /locus_tag="JQR05_002666"
     CDS             8417..8860
                     /gene="holC"
                     /locus_tag="JQR05_002666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY5324808.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8860..11715
                     /gene="valS"
                     /locus_tag="JQR05_002667"
     CDS             8860..11715
                     /gene="valS"
                     /locus_tag="JQR05_002667"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY5324809.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENHESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11769..12965)
                     /locus_tag="JQR05_002668"
     CDS             complement(11769..12965)
                     /locus_tag="JQR05_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY5324810.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGENVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13158..13661
                     /locus_tag="JQR05_002669"
     CDS             13158..13661
                     /locus_tag="JQR05_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5324811.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13707..14123)
                     /gene="rraB"
                     /locus_tag="JQR05_002670"
     CDS             complement(13707..14123)
                     /gene="rraB"
                     /locus_tag="JQR05_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY5324812.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14285..15298
                     /gene="argF"
                     /locus_tag="JQR05_002671"
     CDS             14285..15298
                     /gene="argF"
                     /locus_tag="JQR05_002671"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY5324813.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            complement(15351..16412)
                     /locus_tag="JQR05_002672"
                     /pseudo
     CDS             complement(15351..16412)
                     /locus_tag="JQR05_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4310 a         4089 c         4092 g         3921 t
ORIGIN      
        1 accataatcc cgactcaatc aacgaactac aagccatcgt cctgaagcca tgcccacaaa
       61 cctcgacctt cgtgtcatac cccattacgc gcaaagcctt gttcaccgta ttctcactca
      121 tcggcttacg tggatcgtga tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt
      181 tgatcttttc caaaatactt agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca
      241 tcttcgagcc acgctgagaa tgcttgactc cttccagttg ctcacgctca cctgggattg
      301 tccacatagc cgtttcaaaa tctacttctg accagcgggc aaaacgcagc tcgctcgaac
      361 ggatgaaaac caataaagtg agttcaaccg ccagtcgagt taacggtctg ccagaatagt
      421 gatctatgcg atgaagtaat tcaggtatac ggttcagttc aagagctgca cggtgctgtc
      481 ttttcgccgt agcaaccgca cctgcaatct cttgcgcggg gttgtagtcg attaagccgc
      541 tctgaacggc aaagcgcata atcgcggtag tacgctgctg taaacgggcg gcaacttcga
      601 gccgcccgga tgactcgact gctttaatgg gtacaagaag atcccgcgtc ttcagttccg
      661 caatgttccg cttaccgata gcagtaaaga gattatcctc cagacttttc aaaacacgag
      721 cgctatgcga tgccgaccac ttctgattgc tggcgtgcca gtctctggcg actacttcaa
      781 acgttatcgc ctcttgttcc tgttctacct taacagcttt cttgttttca ctgggatcga
      841 caccattagc taaaagctta cgagcctcat cccggcgtgc cctggcatcc gccaacgaca
      901 cttcggggta tttccccaat gccagcatct tctctttgcc gccaaagcga taacgtagcc
      961 gccagtattt ggagccgtta gggtgaacca gcaaaaccat gccttcaccg tcggtcagct
     1021 tataggcttt tgcttcaggc ttagccgaac gaaccttcac atcactcaga gccatgatga
     1081 gtatcctttc aaggggtctg tgtgggtaca aacattatcg aaccgggaaa tacccgcagt
     1141 tgtacccgca tcagtaagtt gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa
     1201 gcgaggaaag ccttgcggat actggatttt aggcacaaaa aaagacgtcc gttgacgtct
     1261 attgatgttc cgatggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat
     1321 tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac
     1381 gttgtggatt atacctgtcg cacgccacca tgcaagcgcc agcttgcacc cacctcgcta
     1441 agtgctgaaa ttttcagcat tacactcagc gatatgtaac ctttgtcaca ctccaggcac
     1501 cccgccctgc catgctctac acttcccaaa caacaccaga gaaggaccca aaaatgtcga
     1561 tgataaaaag ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgatc
     1621 ccggtgagct gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atttgtcatt
     1681 ccgatctgtc gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc
     1741 atgaggtgat tgggcgcgtg gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg
     1801 tcggtcagcg tgtcggtatt ggctggacgg cgcgtagctg tggtcactgc gacgcctgta
     1861 ttagcggtaa tcagatcaac tgcgagcaag gtgcggtgcc gacgattatg aatcgcggtg
     1921 gctttgccga gaagttgcgt gcggactggc aatgggtgat tccactgcca gaaaatattg
     1981 atatcgagtc cgccgggccg ctgttatgcg gcggtatcac ggtctttaaa ccactgttga
     2041 tgcaccatat cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata
     2101 tcgctataaa acttctgcac gcaatgggat gcgaggtgac ggcctttagt tctaatccgg
     2161 cgaaagagca ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc
     2221 aggcactgaa agccctgtcg gggcagtttg atctcattat caatactgtg aacgtcagcc
     2281 tcgactggca gccttatttt gaggcgctga cctatggcgg taatttccat acggtcggtg
     2341 cggttctcac gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcatct
     2401 ctggttctgc taccggcacg ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca
     2461 gcaaagttgc gccgacaacc gaactgttcc cgatgtcgaa aattaacgac gccattcagc
     2521 atgtacgcga tggtaaagcg cgctaccgag tagtcctgaa agccgacttc tgatcccttt
     2581 gccgggtgct gtcgcccggc acttaaatct ctttggaagg cgtctcgcaa tctgctgaat
     2641 ttagcggtga agagtgcaca gccgttgcct atactccagg caggaaactg gaggaaatct
     2701 catgagcgaa cccctgttaa ttgcccgcac gccggacaca gaactgtttt tactgccggg
     2761 aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa ccgttacgct
     2821 gcaaaaactg gcagagtcat tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa
     2881 aggcgatctg accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct
     2941 taaaaatatc ggcgtcaatg actggcaacc gcatgccaat ccggtggtgg tgtgggatat
     3001 ctttggcgag aaaggccaca cggtgcgggc gacggtttcg gatctggggc cgctgttgct
     3061 ggcgcgactg ttgaatctca acgatgtgca atctggcgtg ctgaatatta tcttccgcat
     3121 tgcggacgat cagggattgt tgctgctcga ctttaaagat ttgcgggcga ttacccagta
     3181 catcggcgat aacgccaaat ccttccagaa tcagtacgga aatatcagta gcgcatcggt
     3241 tggtgccatc cagcgcggat tactgtcgct ggaacagcaa ggcgcagcac acttctttgg
     3301 tgagccgatg ctggatatca aagactggat gcgcaccgat gccaacggta aaggcgttat
     3361 caatatcctc agcgccgaga aactttatca gatgccgaaa ctgtacgccg ccagcctgct
     3421 gtggatgctt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa
     3481 actggtgttt ttcttcgacg aggcacatct gctgtttaac gatgcaccgc aggtactgct
     3541 ggataagatt gagcaggtga taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt
     3601 ttcgcaaaac ccgtctgata ttccggataa tgtgctcggc cagctcggta atcgtgttca
     3661 gcacgctttg cgggctttta cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac
     3721 catgcgggcc aatccggcat ttgataccga aaaggcaatc caggaactgg ggaccggcga
     3781 ggcgttaatc tcgtttctcg atgcaaaagg aagtccttct gtggtggaac gggcgatggt
     3841 gatcgcacct tgttcgcgaa tggggccggt gacggaagat gagcgtaatg gcctgattaa
     3901 tcactccccg gtgtatggca aatatgaaga tgaggtggac cgcgagtccg cctatgagat
     3961 gctgcaaaaa ggctttcagg ccagtaccga gcagcaaaat aatccccccg tgaaagggaa
     4021 agaggtggcg gtggatgacg gtattcttgg tggattgaag gatattttgt ttggcactac
     4081 cggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg
     4141 ccaggtgacg aatcagattg tacgcgggat gttggggagt ttgctggggg ggagaaaaag
     4201 ataagtcaga cgtgatctgt gcccctcacc ctaaccctct ccccaaaggg gcgaggggac
     4261 cgttcgaact cgtatttgac tttgtcaaca ataataaagg gagctttcgc tcccttgtta
     4321 gattacgatt ttctcattaa cagccacagg ctgattaaga agaagctggc acttggcaac
     4381 agtgcgccga tgatcggcgg gatgccgtaa accaacgtca gcgggccgaa gatctggtcc
     4441 agtacgtaga agacaaaacc gaagctgata cctgtgacca cacgcacgcc catcggtacg
     4501 ctacgcagtg ggccaaagat aaacgacagc gccatcagca tcatcaccgc cacggatagc
     4561 ggctggaaga ttttgctcca catgttgagc tgataacgtc cggcatcctg accgctcgac
     4621 ttcagatact tcacgtagtt gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc
     4681 accacgccca gtttgtctgg cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa
     4741 ccggtaatct gtttcggatt ggtcagatca gattcatcaa cctgcgacag acgccagact
     4801 ttatgttccg ggtcaaactt cgcggtagcg gcataacgta cggattgcag acgacgattc
     4861 tcgttaaagg cataaatact gatgccacct aactcctcgt cacctttaac ccgctcaatg
     4921 tagacgaagt tgttgccatc tttcgcccat aaaccttgct gggtagagag caacgagccg
     4981 ccgtacatcg cctgcgcacg gtagttacgc gccatctgtt cgccctgcgg cgcgacccat
     5041 tcgccaatcg ccatcgtcag caagaccagt ggaatggcgg ttttcatcac cgacagcgct
     5101 acctgcatac gggtaaaacc agaagcctgc atcaccacca gttcgctgcg ctgcgccagc
     5161 atcccaagac caagcaacgc cccaagaaga gccgccatcg ggaagaagat ctgcacatct
     5221 ttcggcacgc tcagcaaggt atacattcct gcgcctaacg cgtcgtaact cccctgcccg
     5281 gcttttttca gctgatcgac aaatttgata atgcccgaca gcgacaccag catgaacagc
     5341 gtcatcatga tggtggtgaa aatagtttta ccgatatagc ggtcaagtac gccaaaaggt
     5401 tgcatcacac cgctccttta cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt
     5461 cccaaaggtt gagaacaatc gccaaagcca gataaatcag gttaacggtc cacatccaca
     5521 gtgtcgggtc cagcttacct ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga
     5581 aaagtagata cagcagcatg gctggcagca tcgacagtac gcgtccctga cgcgggttaa
     5641 ccacgctcaa cggtacaacc ataagtgcca tcataaacac ggtgaatacc aacgtgatac
     5701 gccagttcag ttctgcgcga gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca
     5761 tctggtcggt atcgttcggg tcgagcgcca ccgcctggtg accaatgatc gcctgataat
     5821 cctggaaatc cgtaatgcgg aaatcacgta acagtgcagt gccttcgaag cgcgttccct
     5881 ggttgagagt gacgacctgg gagccgtcgc gcagctgggt taaatgtccg gaatcggcca
     5941 ccaccacaga aggacgtgca ttaccttttg gtcgaatttg tgcgaggaac acatctttga
     6001 aatcgctgcc gtcaacgctt tcgatgaaca gcaccgagct gccattagtc gcttgctgga
     6061 attgcccttg cgccagcgcc gccatgccag ggttcgcttt cgcttctgct aacacttcat
     6121 cctgatgacg cgatgaccac ggtcccgccc acatcacgtt aaccgccgcg acgattgccg
     6181 tgaataccgc aaggatcatt gccgctttca ccagaaccgc tttgctcaag ccgcaggcat
     6241 gcattaccgt aatttcactt tcggtataca gtttgcccag cgtcatcagc agcccgagga
     6301 acaggcttaa tggcaggata agctgcgcca tttccggcac gcccaacccg agaagggaga
     6361 gcaccagatt cgccggaata tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt
     6421 gacagaagaa gatcaaaagc aagatgaaga gtatcgccag ctggcttttg agcgtctccc
     6481 gcaccagata tcttatgatt atcactttaa atacgcccgt aaaaactcgt cttttgcagg
     6541 attttagctt gtttcatggc ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc
     6601 taagataatt atactcaacg gattcacctc tcagattttg ttctgacgtg ccaatgccgt
     6661 aataacgtta agattaacac gaagtcatcg caacagcgga catgagttac gaaagcttgc
     6721 aattctatct gtagccaccg ccgttgtctt taagattcag gagcgtagtg catggagttt
     6781 agtgtaaaaa gcggtagccc ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc
     6841 gaaccacgtc gcctttctcc gattgcagaa cagctcgata aaatcagcga tgggtacatc
     6901 agcgccctgc tacgccgggg cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac
     6961 catgttccga atgtactttc cgagcgaatt ctccttattg gttgcggcaa agaacgtgag
     7021 ctggatgagc gtcagtacaa gcaggttatt cagaaaacca ttaatacgct gaatgatact
     7081 ggctcgatgg aagcggtctg ctttctgact gaactgcacg ttaaaggccg taacaactac
     7141 tggaaagtgc gtcaggctgt cgagacagca aaagagacgc tctacagttt cgatcagttg
     7201 aaaacgaata agagcgaacc gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc
     7261 cgccgtgaac tgaccagcgg tgagcgcgcg atccagcacg gtctggcgat tgccgccggg
     7321 ataaaagcag cgaaagatct cggtaatatg ccgccgaata tctgtaacgc cgcttatctc
     7381 gcctctcagg cgcgccagct ggctgacagc tacagcaaga atgtcatcac ccgcgttatc
     7441 ggcgaacagc agatgaaaga gctgggaatg cactcttatc tggcggtcgg tcagggttca
     7501 caaaacgaat cgctgatgtc ggtgattgag tacaaaggca acgcgtcgga agatgcacgc
     7561 ccaatcgtgc tggtgggtaa aggtttaacc ttcgactccg gcggtatctc catcaagcct
     7621 tcagaaggca tggatgagat gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg
     7681 atgcgtatgg tcgcggagct gcaactgccg attaacgtta ttggcgtgct ggcaggctgt
     7741 gaaaacatgc ctggcgggcg tgcctatcgt ccgggcgatg tgttaaccac catgtccggt
     7801 caaaccgttg aagtgctgaa caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta
     7861 acttacgttg aacgttttga gccggaagca gtgattgacg tggcgacgct gaccggtgcc
     7921 tgcgtgatcg cgctgggtca tcatattact ggtctgatgg cgaaccataa tccgctggcc
     7981 catgaactga ttgccgcgtc tgaacaatcc ggtgaccgcg catggcgctt accgctgggt
     8041 gacgagtatc aggaacagct ggagtccaat tttgccgata tggcgaacat tggcggtcgt
     8101 cctggtgggg cgattaccgc aggttgcttc ctgtcacgct ttacccgtaa gtataactgg
     8161 gcgcacctgg atattgcagg aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt
     8221 cgtccggtag cgttgctggc acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag
     8281 taagtttgcg ttttaccctc accctaaccc tctccctaag ggagagggaa ccgatcgagc
     8341 gcaatagaaa ttcattgctc atctttcagg gtgaggaatg cagcaatatt taaatccacc
     8401 acaagaagcc ccatatatga aaaacgcaac gttctacctt ctggacaatg acaccaccgt
     8461 cgatggctta agcgccgttg agcaactggt gtgtgaaatt gccgcagaac gttggcgcag
     8521 cggtaagcgc gtactcatcg cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc
     8581 cctgtgggcg cgtccggcag aaagctttgt tccgcataat ttagcgggag aaggaccgcg
     8641 cggcggtgcg ccggtggaga tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat
     8701 attgattagt ctgcgaacaa gctttgcaga ttttgccacc gctttcacag aagtggtaga
     8761 cttcgttcct tatgaagatt ctctgaaaca actggcgcgc gaacgctata aagcctaccg
     8821 cgtggctggt ttcaacctga atacggcaac ctggaaataa tggaaaagac atacaaccca
     8881 caagatatcg aacagccgct ttacgagcac tgggaaaagc agggctactt taagcctaat
     8941 ggcgatgaaa gccaggaaag tttctgcatc atgatcccgc cgccgaacgt caccggcagt
     9001 ttgcatatgg gtcacgcctt ccagcaaacc atcatggata ccatgatccg ctatcagcgc
     9061 atgcagggca aaaacaccct gtggcaggtc ggtactgacc acgccgggat cgctacccag
     9121 atggtcgttg agcgcaagat tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc
     9181 gaagctttca tcgacaaaat ctgggaatgg aaagcggaat ctggcggcac cattacccgt
     9241 cagatgcgcc gtctcggcaa ctccgtcgac tgggagcgtg aacgcttcac catggacgaa
     9301 ggcctgtcca atgcggtgaa agaagttttc gttcgtctgt ataaagaaga cctgatttac
     9361 cgtggcaagc gcctggtaaa ctgggacccg aaactgcgta ccgctatttc tgacctggaa
     9421 gtggaaaacc acgaatcgaa aggttcgatg tggcacatcc gctatccgct ggctgacggc
     9481 gcgaaaaccg cagacggtaa agattatctg gtggttgcga ccactcgccc ggaaaccctg
     9541 ctgggcgata ccggtgtagc tgttaacccg gaagatccgc gttacaaaga tctgattggc
     9601 aaatatgtca ttctgccgct ggttaaccgt cgtattccga tcgttggcga cgaacacgcc
     9661 gacatggaaa aaggcaccgg ctgcgtgaag atcaccccgg cgcacgactt taacgactat
     9721 gaagtcggta aacgtcacgc cctgccgatg atcaacatcc tgacctttga cggtgatatc
     9781 cgtgaaagcg cccaggtgtt cgataccaaa ggtaacgaat ctgacgttta ttccagcgag
     9841 atcccggcag agttccagaa actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg
     9901 gttgatgcac tcggcctgct ggaagaaatt aaaccgcacg acctgaccgt tccttacggc
     9961 gaccgtggcg gcgtggttat cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat
    10021 gtactggcga aaccggcagt tgaagcggtt gagaacggcg acattcagtt cgtaccgaag
    10081 cagtacgaaa acatgtactt ctcctggatg cgcgatattc aggactggtg tatctctcgt
    10141 cagctgtggt ggggtcaccg tatcccggca tggtatgacg aagcgggtaa tgtttatgtt
    10201 ggccgcaacg aagaagaagt gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt
    10261 caggacgaag acgttctcga cacctggttc tcttctgcac tgtggacctt ctctaccctt
    10321 ggctggccgg aaaataccga cgccctgcgt cagttccacc caaccagcgt gatggtatct
    10381 ggtttcgaca tcatcttctt ctggattgcc cgcatgatca tgatgaccat gcacttcatc
    10441 aaagatgaaa atggcaaacc gcaggtgccg ttccacactg tttacatgac cggtctgatt
    10501 cgtgacgacg aaggtcagaa gatgtccaaa tccaagggta acgttatcga cccgctggat
    10561 atggttgacg gtatttcgct gccagaactg ctggaaaaac gtaccggcaa tatgatgcag
    10621 ccgcagctgg cggacaaaat ccgtaagcgc accgagaaac agttcccgaa cggcattgag
    10681 ccgcacggca ccgatgccct gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac
    10741 atcaactggg atatgaagcg tctggaaggc taccgtaact tctgtaacaa gctgtggaac
    10801 gccagccgct ttgtgctgat gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa
    10861 atgacgctat cgctggcgga ccgctggatt ctggcggagt tcaaccagac tatcaaagcg
    10921 taccgcgaag cgctggacag cttccgcttc gatatcgccg caggcatttt gtatgagttc
    10981 acctggaacc agttctgcga ctggtatctg gagctgacca agccggtaat gaacggtgga
    11041 acagaagccg aactgcgcgg tactcgccat acgctggtga ctgtactgga aggtctgctg
    11101 cgcctcgcgc atccgatcat tccgttcatc accgaaacca tctggcagcg tgtgaaagta
    11161 ctttgcggta tcactgccga caccatcatg ctgcagccgt tcccgcagta tgatgcatct
    11221 caggttgatg aagccgcact ggccgacacc gagtggctga aacaggcgat cgttgctgta
    11281 cgtaacatcc gtgcagaaat gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt
    11341 ggttgcagcg cggatgcaga acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg
    11401 gcgcgtctgg aaagcatcac cgtgctgcct gccgatgaca aaggtccggt ttccgttacc
    11461 aagatcatcg acggcgcaga gctgctgatc ccgatggctg gcctcatcaa caaagaagat
    11521 gagctggcgc gcctggcgaa agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag
    11581 aacaagctgg caaatgaagg ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag
    11641 cgtgagaagc tggaaggtta tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt
    11701 atcgccgcac tgtaatcaac acagtgatga aaacgaaggc cggagcatgc tccggccttt
    11761 tttatctctt acagaaatgg taaagaaggc ataactcccc gagaaatact ggctaagaag
    11821 cctttatctg gttgttcttc tttattgatt aaaggaaggt catccaaatc gcccagcaaa
    11881 tacgtatttt ttgcctgcca gtcaatcatc catattttca gcagtgggta agccagacca
    11941 cccgtaatcc cggatatcac caccaacgca cacatgcgat aaagcatacc gtggtacgtt
    12001 aaagttgagc gaaaacgaat acgcccatca ttcagtgaca ggttgctcat aaaatgattt
    12061 cgcaacgaca ccgttaaata actggtgcta acagcaatcc caaaataata aattaactgc
    12121 gcgattatca ttttacgttg catttccata aattgctgta aattctcaat atcatcattt
    12181 gcatacccag aactatcata ctcatttaat atttgatcga agataatata accagcaaca
    12241 gcgagaaaag gtaataaagc taaaaatgcg aggatggcat attttataca atacgcagta
    12301 tctaatttca ccttgaaacg atgtataccg aaactggtat tgctccagag aaaactcatt
    12361 accaggctat ataaagtacc attaaaaata ccaatggaaa caataccaac tattgccatc
    12421 agaaccacag atataataac actacttgaa ctattggcat gtagcatctt tgtcgagata
    12481 aagaaaacag tccccatccc aatggccatt aaaatcggca agaaaaaggt tacccaccag
    12541 aaccctttca aagagcaatt aaaactaaat cttacgccgt tgagactggt cattaaggcc
    12601 tgataacgca gtccttttgc tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa
    12661 acacagccaa caagcggcat atctgctgac actgtcatta agattgcgaa atagaaaaaa
    12721 acaaaaacaa gacaactaac aaaaacattc tcaccggtaa ttccataaga aaatcgttgt
    12781 ccgttaactt ccatattagc ataaagataa cgcttacatt tcattaatgc ccatggtaaa
    12841 tagataccta gcgttataat cgttaacaac acattcacca cacaaataag aaaatatctc
    12901 tcacctgtac catgaaaaac aaaagaatgg gacggaacat ccatttcatt aataacttga
    12961 gccataaaaa tcccctgaat taaaaaatcc attgatataa tttatagata cagacagtaa
    13021 aatgttctgc cacgttacaa tactcttatc agtaataggc tggcaaaaac tttattgctt
    13081 tataagacgc ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat
    13141 ttttattggg ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga
    13201 cgctgcagga taatcctgct atcgccagcg tcatccgtca ggtttacgca gagtatggtc
    13261 ttaccgccga taaaggctac accgttgccg atccgaatct cgacgaactg tatcaagtgt
    13321 acagccagcc tggccatgct tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg
    13381 gcgggattgc gccattaaca ggtagtgaag cggacatttg cgaactgcag aaaatgtact
    13441 ttcttcccgc tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc
    13501 aggcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag
    13561 ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg
    13621 gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg
    13681 tgctatcggg cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca
    13741 aaatcttcat cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg
    13801 tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga
    13861 tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca
    13921 acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct
    13981 ttttccagag tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc
    14041 ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct
    14101 tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt
    14161 gtcacacacc actgacattg cctccacctt tgtgacaaag atttatgctt tagacttgca
    14221 aatgaataat catacatata aattgaattt taattcattg aggcgttagc cacaggaggg
    14281 atctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga
    14341 actcaacagc ctgctgcagt tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga
    14401 agaagccaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgactcgtac
    14461 ccgatgctct ttcgaagttg ccgcatatga ccagggtgct cgcgttactt atcttggccc
    14521 aagcggtagt cagattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg
    14581 catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga
    14641 atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagttgct
    14701 ggcggatctc ctcaccatgc aggagcattt acccggtaaa gcattcaacg aaatgacgct
    14761 ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcggcgct
    14821 taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt
    14881 tacggaatgc cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt
    14941 agctaaagga gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga
    15001 agcaaaagaa aaatgggctg aacgcattgc attactgcgt gattatcagg tgaacagcaa
    15061 gatgatgcag ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca
    15121 tgacgaccaa acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat
    15181 ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa
    15241 ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaattaa ataattaata
    15301 tctctataaa gatgccggga aatacaaaaa catcccggca tcaaattatt ttatgcagtg
    15361 atagtttttt tcttccagcc aaatttttct attgccatac gtaaattttc cggaggattt
    15421 agtgtattta aacgctgttg gtcaatatat ggcgatacat taaccttcga tttaaggata
    15481 gtttgcatca ttctaaatct aagtttgaat tcattattca gtttttcttt gataacatta
    15541 tctggttgat tcttcaaaat ttctttatta gatttctcat catttatatc atattttaaa
    15601 ttatcaaaat aatttttttc gccctcataa ctcttaagta accgaggaaa gtaccagtta
    15661 atcatctcag tgtttttcat aaaaaaatca ttcagaaaaa tatcattaac taaaatgctg
    15721 acaggtactt tattaaatag cgactcaaca ttattaaata tcctttgtat taaatatatt
    15781 ttttctcgat tgcttaagtt aaaatcattg acatatacaa ccaacttttt ccaaatcaac
    15841 tcctgtaatt cagggctatt tttatagggt tctttatcga gattgaaaaa taatgtctca
    15901 gctactttag actgatcgca gagaaaatta atatgattta ttacggagca tgcatgctct
    15961 atctttgcag aatcatcaaa atattgcatg cttttattaa aacaccactc cagtattttt
    16021 acatcattat tatatggcaa ttctaaaaaa gtcaccaaca tcgaatggat gaccggtgta
    16081 aggttaacct ttgcatcatc taaggaatca ataagaatat gagacaggtt aatcatgatg
    16141 tcattaattt cattgttacc ttctttgtca ttatcaattc cgtgatcatt taattcgtag
    16201 agcttatcta tttcttcaag tataccattt tcgcccagta agtatgaaat ctattcatca
    16261 catacatgag gcattaatag cagatgttcg ttccttattg ctaataaggc tttattcaat
    16321 gctttttcta ataaatccgt aatcgtagaa tcaactgaac ctttacaact cacctgcgag
    16381 ttatttaact cctgagaatc atttaactcc tg
//
LOCUS       DADKXM010000086        15936 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.087, whole genome shotgun sequence.
ACCESSION   DADKXM010000086 DADKXM010000000
VERSION     DADKXM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.087"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /gene="glxK"
                     /locus_tag="JQR05_002673"
     CDS             <1..1064
                     /gene="glxK"
                     /locus_tag="JQR05_002673"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY5324814.1"
                     /translation="TLFPDAHYICLPIADGGEGTVDAMVAATGGNIVTLEVCGPMGEK
                     VNAFYGLTGDGKTAVIEMAAASGLMLVAPEKRNPLLASSFGTGELIRHALDNGIRHII
                     LGIGGSATVDGGMGMAQALGVRFLDADGQVLAANGGNLARVASIEMEECDPRLANSHI
                     EVACDVDNPLVGARGAAAVFGPQKGATPEMVEELEQGLQNYARVLQQQTEINVCQMAG
                     GGAAGGMGIAAAVFLNADIKPGIEIVLNAVNLAQAVQGAALVITGEGRIDSQTAGGKA
                     PLGVASVAKQFNVPVIGIAGVLGDGVEVVHQYGIDAVFSILPRLAPLAEVLASGETNL
                     FNSARNIACAIKIGQGIKN"
     gene            complement(1292..2077)
                     /gene="allE"
                     /locus_tag="JQR05_002674"
     CDS             complement(1292..2077)
                     /gene="allE"
                     /locus_tag="JQR05_002674"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY5324815.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGTSFVDYLVTLHQNGGNQQCFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPSSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(2088..3323)
                     /gene="allC"
                     /locus_tag="JQR05_002675"
     CDS             complement(2088..3323)
                     /gene="allC"
                     /locus_tag="JQR05_002675"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY5324816.1"
                     /translation="MITHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FADAMKACEFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(3345..4394)
                     /gene="allD"
                     /locus_tag="JQR05_002676"
     CDS             complement(3345..4394)
                     /gene="allD"
                     /locus_tag="JQR05_002676"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAY5324817.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKVAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            4711..6378
                     /locus_tag="JQR05_002677"
                     /pseudo
     CDS             4711..6378
                     /locus_tag="JQR05_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
     gene            6388..7647
                     /locus_tag="JQR05_002678"
     CDS             6388..7647
                     /locus_tag="JQR05_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY5324818.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            7658..8473
                     /locus_tag="JQR05_002679"
     CDS             7658..8473
                     /locus_tag="JQR05_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY5324819.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            8470..9363
                     /locus_tag="JQR05_002680"
     CDS             8470..9363
                     /locus_tag="JQR05_002680"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY5324820.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9500..10567)
                     /gene="purK"
                     /locus_tag="JQR05_002681"
     CDS             complement(9500..10567)
                     /gene="purK"
                     /locus_tag="JQR05_002681"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY5324821.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWLAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(10564..11073)
                     /gene="purE"
                     /locus_tag="JQR05_002682"
     CDS             complement(10564..11073)
                     /gene="purE"
                     /locus_tag="JQR05_002682"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY5324822.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(11191..11913)
                     /gene="lpxH"
                     /locus_tag="JQR05_002683"
     CDS             complement(11191..11913)
                     /gene="lpxH"
                     /locus_tag="JQR05_002683"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY5324823.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(11916..12410)
                     /gene="ppiB"
                     /locus_tag="JQR05_002684"
     CDS             complement(11916..12410)
                     /gene="ppiB"
                     /locus_tag="JQR05_002684"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY5324824.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            12584..13969
                     /gene="cysS"
                     /locus_tag="JQR05_002685"
     CDS             12584..13969
                     /gene="cysS"
                     /locus_tag="JQR05_002685"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY5324825.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(14005..14526)
                     /locus_tag="JQR05_002686"
     CDS             complement(14005..14526)
                     /locus_tag="JQR05_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5324826.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWRVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(14634..14846)
                     /gene="ybcJ"
                     /locus_tag="JQR05_002687"
     CDS             complement(14634..14846)
                     /gene="ybcJ"
                     /locus_tag="JQR05_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY5324827.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(14848..15714)
                     /gene="folD"
                     /locus_tag="JQR05_002688"
     CDS             complement(14848..15714)
                     /gene="folD"
                     /locus_tag="JQR05_002688"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY5324828.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAARLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         3696 a         4127 c         4437 g         3676 t
ORIGIN      
        1 cgaccctctt tcccgatgcg cactatatct gtttgccgat agcggatggc ggcgaaggga
       61 cggtggatgc gatggtcgcc gcgacgggtg gcaacatcgt gacgcttgaa gtctgcgggc
      121 cgatgggcga aaaagtgaat gctttttatg gccttaccgg agacgggaaa acagcggtga
      181 ttgagatggc ggcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt aatccgttgc
      241 tggcctccag ttttggtacg ggggagttaa ttcgccatgc gctggataac ggcattcgcc
      301 atattattct cggcattggc ggcagtgcga cggtcgacgg cggtatgggc atggcgcagg
      361 cgcttggtgt gcgtttcctt gatgccgacg gccaggtgct ggcggctaac ggcggcaatt
      421 tagcgcgcgt ggcaagcatt gagatggagg aatgcgatcc gcgtctggcg aacagccata
      481 ttgaagtggc atgtgacgtt gataacccgc tggtaggggc acgcggcgcg gcggcggtat
      541 ttggcccgca aaaaggggca acgccggaga tggtcgaaga gctggaacag gggctgcaaa
      601 attacgcccg tgttttacaa cagcaaactg aaattaatgt ctgtcagatg gcgggcggcg
      661 gcgctgcggg cggtatgggt attgcggcgg cggtatttct caatgcggat attaaaccgg
      721 gcattgagat tgtgttgaat gcggtcaatc ttgcgcaggc ggtgcagggg gcagcactgg
      781 tgatcaccgg ggaagggcgc attgactcgc aaacggcagg cggtaaagcg ccgctgggcg
      841 ttgcgtcggt ggcgaagcag tttaatgtac cggtgattgg gattgctggt gtattgggtg
      901 atggcgtgga agtggtgcac cagtacggca ttgacgcggt attcagcatt ttgcctcgtc
      961 tggcaccttt agccgaagtg ctcgccagcg gtgaaaccaa tctcttcaac agcgcgcgaa
     1021 atattgcctg cgccattaaa ataggtcagg gaattaaaaa ctgactcttg cctttaaagc
     1081 ggatgcgatt tatttcgtat aagagcgcag tactcatgcc ggatgcggca tgagtactat
     1141 atccttcctg aaaatcgcgc aaattctata tattgcagag gtcatgtagg cctgataagc
     1201 gaagcgcatc aggcaatgtt acaaaaaaag ccacggtata aaccgtggca aaatccaaca
     1261 tagctaaaaa taatcaggcg agtggtatga cttaaatctc tacgtcgcgg ttgcaatctt
     1321 tcgagtaaat atagctgaac gcttcaccac gccctacgcc ataaccagcc tgtaaagaat
     1381 aagcgcccat aaagatgtaa tcgccttttt tcaccgggat ccagttattg tcgaggttat
     1441 aaaccccctg accggaaaga atataggcac catgttcctg aacgtgtgtt tcgatataac
     1501 cgtggctggc acctggtgca aaagagagga tatgcatgtt catatcaaaa cctaactctt
     1561 tgggcagaaa atccagcagg atcacatcat ccataccttc atagtgaatg cgttccagtt
     1621 cgctggcatt gccagaaacc agccacggtg catggccttc taccggaaca tagcggcgct
     1681 tatataaaaa gatttggctg tcttcggcct gggcgttaac aaacgtcatt aaggagcttg
     1741 gtgggcaata aagatagcca ccttcgctta aggcaaatgt tttgccttcg gctttggcag
     1801 tgatatttcc agagatcaca tacaggaacg tttcaatgcc ttcgccaccg aagcactgtt
     1861 ggttgccacc gttttgatgc agtgtgacca gataatcaac aaaagaggta cccagctttg
     1921 gcgtggagag gattgtcgcg tcacaatttt caaagcccgg aataatattt tttaccagac
     1981 catccggggt taacagtgcg aaattaccgt gtttaacaat cgcacggtta gccagtaaat
     2041 cttcgcggta accggtgacg ttatttaaat atcccattta tgactcctta tttctgccag
     2101 gcaagttgat aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc agtaatattg
     2161 gtgcgttccg ccgggttatg gctgatcccg ttgatgctgg ggataaaaat catgcaggtt
     2221 ggtacgcgag gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat cacccggtaa
     2281 ttcagttttt ctctttcaca caattctgtc agggtggcga ccagctcctt attcatcggc
     2341 acgggttctt cgtccatcca taaatcgata tcaataccaa tgtccatttc atcgcaaatc
     2401 gcccgcatgt cgttttctaa ctgttgggtg aaatcgcgca gcacggcagc gtcggtatga
     2461 cgacaatcaa tggtgaacgt ggttttaccc ggcaccacat ttaccgtatt cgggcgcggc
     2521 tctactttgc caaaggtcag aaccagcgga tcgcccatct ttttcgcttt ttcgatcgac
     2581 tgatggcaaa tgcgactgaa agcgtaaacc gtatcacgac gataacccat cggcgtggtg
     2641 cctgcatggt ttgattcgcc gttcagcgtc accgtatagc gacgctgccc gacaattgca
     2701 ttcaccacgc caattgattg cccattactt tccagcacac agccctgttc aatatgcagt
     2761 tcaacaaagg cttttatatc ctgacgcgga gttagtgggg cgttcggcag agtaaattcg
     2821 caagccttca tcgcatcggc aaaactattt cctttggcat cacagatatt ccgcacgtcg
     2881 tcaggattcg ccagcccaaa gatattttta ctgccccaga agacatacgg gaagcggctg
     2941 ccttcttctt ctgccatcgc caccacttcg accgtacgta acggtgcgcc gtattgcgtt
     3001 ttcaaccagt caattgccag ccacgccgcc agcgcgccga attgcccgtc gaggttaccg
     3061 ccgttaacca cggtatcgat atgcgaaccg ctcagaacca cttcctgtgg atattcggtg
     3121 ccactcaggc gaccgtataa attccccact tcatcgaaac gtgtttccag cccacttgct
     3181 gccattcttt ttttaaattg ctgctgggtt tccagccatt ccggcgaata aagtaaacgg
     3241 gtcatcccac ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt ttcttctata
     3301 gcttgacggg aatgtgtaat cataagaaag tcctgtctca ataattattg cgcaaaggga
     3361 tttttcgttt cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg gtaaatatca
     3421 tcaacaattt cgatgccttc gacggcgact ttgcgttgtt taatatcctg atcctgtccg
     3481 ggataataaa cctgattaaa accgggcgcg ggggtaatgg catttaattc gcgcatggtc
     3541 tggctaagat gttgacggaa taattcgctg gaggagaaaa agttcggatt aataactata
     3601 tgtaattgcc ccaaattacg ccctgcgtgt aaatcgtcat acatcgaact aacctgtcgc
     3661 ccgaacggta agccgagtaa gacgcctgag aggacgtcaa tcatcatcat caggccatac
     3721 ccttttggcc cagcggcggg gagcagagca tgtaccgcga acggatcggt tgttggtgca
     3781 ccgtttttat cgaccgccca ggtatccggg atagacatat tacgcgagcg ggcgtcgagc
     3841 acttttcccc atgcctgtac ggtagtcgcc atatcaaagg taaggatctc gtcgccttct
     3901 cccggtgcgg caaaggccag tgggttagta ccgtagtaaa tttccgcgcc gccaaacggc
     3961 accaccattg gatcggactg gcacatcgaa atgccaatta atccggcgcg ggctgcctgc
     4021 tgcacaaaat aagagattgc gccgctgtga cccatccggc tgataccgac caccgcaacg
     4081 cctttttgct gggcggtttt gatggcatgt tccataccca ttttcgccgc gacctgcccg
     4141 gcggcattgt cagcatgtaa aattgccgag cacggcccgg tttcctcaag acgaaactcc
     4201 ggttcgcggt tggtgccgcc ttttgaaatg cgttccgcgt agtattccac gcgcaccgcg
     4261 ccatgagagt ggatccctct ggcatcggcg taaaccaata cttcagccac ggttgcagcg
     4321 tgctcacgtt ttaacccagc ctggcagagt ttattctcga ttagctggtg gagtgtttcc
     4381 cgactaattt tcatctgtct tcctttttaa cgacggtgtg aagcatgact gcaattaaca
     4441 tacagggaaa atatctggat tatgtgatcc agacaggcaa aaaaatatag ttagaattta
     4501 tttgataatc cgctcgcttt taacctggtt tttaaaacaa ccacgcttat taaaaaataa
     4561 cacgtaatag aatggcggtt atttgaattc ttttgttaat aatccttgtg tgatattcat
     4621 caccttattt acccgttgtc atcgataccg taatcgccac attaacactg ctcgtgcaat
     4681 tgccatgggt gcaattttta aggagttgtt atgatccacg cctttattaa aaaagggtgt
     4741 tttcaggatt cggtcagttt aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt
     4801 gatgatgttt ccgtaatgat gggtacgccc gccaataaag cgttattaga taccacaggt
     4861 ttctggcatg acgattttaa taacgccacg ccgaacgata tttgcgtggc aattcgtagc
     4921 gaagcggcgg atgcggggat cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa
     4981 caactggcgc agggttcagg cagcagccag gcgttgacgc aggtgcgtcg ctgggacagc
     5041 gcctgtcaga aattacccga tgccagtctg gcgctgattt cagtggctgg cgagtatgcg
     5101 gcggagctgg cgaaccaggc gctggatcgc aacctcaacg tgatgatgtt ctccgataac
     5161 gtcacgctgg aagatgaaat ccaactcaaa acccgcgcgc gggaaaaagg cttgctggtg
     5221 atggggccgg actgcggtac atcgatgatt gccgccacgc cgctggcttt tgctaacgtg
     5281 atgccggaag gcaacattgg cgtcattggc gcttccggta ccgggattca ggagctgtgc
     5341 tcacagattg cgctggcagg ggagggaatt actcacgcga ttggccttgg cgggcgcgac
     5401 ctcagccgtg aagtgggcgg catcagtgcg ctgacggcgc tggaaatgct cagtgcagac
     5461 gagaaaagcg aagtgctggc atttgtttca aaaccacctg ccgaagctgt gcgtctgaaa
     5521 attgttaatg ccatgaaagc aaccggcaaa ccgacggtgg cgctgttttt aggttatacc
     5581 ccggcggtgg cacgcgacga gaatgtctgg tttgcctcct cgctggatga ggccgcacgc
     5641 ctggcttgcc tgctttcacg cgttacggcg tgacgtaacg cgataacgcc tgccagcagc
     5701 ggatttattt gcggtttgta taccggcggt acgctggctg ccgaagcggc gggattactt
     5761 gccggatacc ttggcgtgga agtcgatgat gcccatcatc acggcatgat gctcgacgcc
     5821 gatggtcacc agattattga cctgggcgat gatttctaca cagtcgggcg tccccatccg
     5881 atgatcgacc caaccttacg caaccagtta attgccgatc tcggcgctaa accacaagtg
     5941 cgcgtattgc tacttgatgt cgtgattggc ttcggtgcga ctgccgatcc tgccgcctcg
     6001 ctggtaagcg cctggcaaaa agcctgtgcc gcgcgttcag ataatcaacc actgtatgcc
     6061 attgccacgg tgacaggcac tgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg
     6121 ctggaagatg cggggatcgc ggtagtgagt tcgctgccgg aagccacctt gctggcggca
     6181 gcgttaattt atccgctctc gcctgccgca cagcaacata caccgtcatt actggaaaac
     6241 gtcgccgtga ttaacatcgg attacgcagc tttgcgctgg agctacaaag cgccagcaaa
     6301 ccggttgtgc attaccaatg gtcgccagtc gccggcggca ataaaaaact ggctcgttta
     6361 ttagaacgtt tgcaataagg ggttcccatg tttacatcag tggcgcaagc caatgctgcg
     6421 gttatcgaac aaattcgtcg tgctcgtcca cactggctgg atgtgcaacc ggctttttca
     6481 cttatcagcg aactaaacga gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg
     6541 caggagatga ccggacccat gaaaggggcg tgcgtgggcg catgtctgtt cgaaggctgg
     6601 gcgaaagatg aagcgcaggc actggcgatg ctggagcagg gggaagtgaa cttcattcct
     6661 tgtcaccatg tgaatgccgt cgggccaatg ggcggcatta cttctgccag tatgccgatg
     6721 ctggtggtcg agaacgtgac cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc
     6781 ggcaaagtga tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca tcgctggatg
     6841 cgcgatgtgt taatgccagt attaagcgcg gcgctggggc gcatggagcg cggtatcgat
     6901 ctcacggcga tgatggcgca gggcattacg atgggcgatg agttccatca acgcaatatt
     6961 gcttcctcag cactgttaat gcgtacgctg gccccacaaa ttgctcgcct cgatcatgat
     7021 aaacagcaca ttgcggaagt gatggatttc ctcagcgtga ccgatcagtt cttcctcaac
     7081 ctcgcgatgg cctactgcaa ggcggcgatg gatgctggcg cgatgatccg cgcaggcagc
     7141 atcgttacgg ctatgacccg caacggcaat atgttcggga ttcgggtaag cgggctgggt
     7201 gaacgctggt ttactgcgcc tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg
     7261 caggagcagg cgaacccgga tatgggcgat agcgcgatta ccgaaacctt tggtatcgga
     7321 ggtgcggcaa tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg tggcatggaa
     7381 gcggccaggg cggtatctga agagatggcg gaaatttacc ttgaacgcaa tatgcagttg
     7441 cagatccccg gctgggattt tcagggcgcg tgcctggggc tggatattcg tcgcgtggta
     7501 gaaaccggca ttacgccact catcaatacc ggtatcgccc ataaagaggc ggggatcggg
     7561 cagattggcg caggcaccgt gcgggcaccg ctggcgtgct ttgaacaggc gctggaagca
     7621 ctggctgaaa gcatggggat tggttgagga acgcgcaatg acgatcatcc atcctctgct
     7681 tgccagcagt agcgcaccga attatcgcca gtcctggcgg ttagcgggag tgtggcggcg
     7741 ggcgattaac ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc agggtagtgg
     7801 tttcggcccc ggaggatggg tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt
     7861 atgcggcaat gaacgaccac aggttgtggc tcaagggatt cgcctcgggc gtttcacggt
     7921 taaacagcca cagcgttatt gtttgctgcg tattacgccg ccttcgcatc ctcaaccgct
     7981 tgcagctgca tggatgcaac gcgcggagga aaccggactt ttcgggccac tggcgatggc
     8041 ggcaagcgat ccgctgcccg ctgaattacg ccagtttcgt cactgttttc aggccgcgct
     8101 caatggcgtt aagaccgact ggcggcactg gctgggtaaa ggccccggat taacgccaag
     8161 tcatgatgac acgttgagcg gaatgctgct ggcggcctgg tattatggcg ctttagatgc
     8221 gcgcgccggt cgcccgtttt ttgcctgttc cgacaatctt caactcgtta ccacagcggt
     8281 gagcgtcagt tatttacgtt atgccgcgca aggatatttc gcctcgccac tcctgcactt
     8341 tgtccatgct ctgagttgcc cgaaacgtac cgctgttgcg attgattcgc tgctggcgct
     8401 ggggcatacg tcaggggcag atacgctgct ggggttctgg cttggccaac aattattaca
     8461 aggaaaacca tgaaaacact ggttgtagct cttgggggca acgccttact ccagcgcggt
     8521 gaggcgctga cggcggaaaa tcaatatcgc aatatcgcca gtgctgtacc cgcgctggca
     8581 cgcctggccc gttcttatcg gctggcgatt gttcacggca acgggccgca ggtggggctg
     8641 ctggcgttac agaatctggc gtggaaagag gtagaaccgt atccgctgga tgtgctggtg
     8701 gcggaaagcc aggggatgat tggctatatg ctggcgcaga gcttgagcgc acagccgcag
     8761 atgccgcctg ttacgacggt gctgacgcgc attgaggttt cgcctgatga tccggcgttt
     8821 ttgcagccag agaaatttat tggtccggtt tatcagccag aagaacaaga ggcactggaa
     8881 gcggcttacg gctggcagat gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct
     8941 ccgcaaccgc gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa agaggggcat
     9001 gtggtgattt gcagtggcgg cggcggtgtg cctgtgacgg aagatggggc agggagtgaa
     9061 gcggtgattg ataaagatct cgccgctgcg ttgctcgccg agcagattaa tgccgatgga
     9121 ctggtgatcc tcaccgatgc tgatgcggta tatgaaaact ggggaacgcc gcagcaacgt
     9181 gccattcgcc atgccacacc ggatgagtta gcgccatttg ccaaagccga tggttcgatg
     9241 gggccgaagg taacggcggt gagtggttat gtcagaagcc gtagtaaacc cgcgtggatt
     9301 ggggcgttat cgcgaattga agagacgctg gcgggcgaag cggggacctg tatttcgctg
     9361 tagtgatgag agcatccggc aatcggtgca ccatgcctga tgcgacgctt gacgcgtctt
     9421 atcaggccta caatgggtac cggagcggta agccggataa ggcgtttacg ccgcatccgg
     9481 caatcaaatg agcacctgct taactgaact tactctgcgc ccaaatcacg ccgctggcat
     9541 actctggcgg cagcagcggg attaaggctt ccagcgtcgc agtcagacgc gatgtgtcgc
     9601 tgtcggtcaa attcagatgc cccactttac gccccggacg gacttctttg tcgtaccagt
     9661 gcagatgcac cagcggcaat ttcagccagt cataattcac atcgctacca atcagattga
     9721 tcatcaccga cggattattc actactggtt gcggtaacgg cagatcggta atcgcccgca
     9781 gatgcagctc aaactggctg atgctggcac cgttttgtgt ccagtgaccg ctgttatgca
     9841 cacgcggagc cagttcgttg atcagcagac cttgcggggt gacaaaacac tccatcgcca
     9901 tcacgcccac atagcccagc tcctgcataa tcgccgacag catctcttcg gcttgcgcct
     9961 gctgctgtgc gttggcctgc ggaaaagcga cgctggtgcg caaaataccg tcctgatgca
    10021 ggttatgcgt cagcggataa aacacggtgc tgccatcaaa gccgcgcgcg ccaaccagcg
    10081 atacttcacc agagaagtta atgccctgct cgacaataca ttcaccgtaa cactctgccg
    10141 gtaactgttc ggtttcattg gcgcgtaaac tccattgacc gcgaccgtca tagccaccag
    10201 tgcgacgctt aacaatcgcc agttcaccta aacgatcaaa caccgcaagc cactcgctgc
    10261 gatcggcaag taactgccac ggcgcggtcg gcaggtggag cttatcgaaa agctgtttct
    10321 gagtcagacg gtcagcaata atcgggaaca catcgcggtt cacaaaggcc ggatgacgcg
    10381 ccagctcgcg ggttaatgcg gtttccggcc agcgttcgat ctcagcggta atcacgcttt
    10441 gttgaaaagg caccgccgcc ggttcagcgt ccagcccgac cggccagaca gcaatgccta
    10501 acggttcgcc tgcctgacgc agcatacggc ctaactgccc gttaccgagg acgcaaacct
    10561 gtttcatgcc gcacctcgcg ggtccgggtt ttccagcact tcgtcggtct gggctttgcg
    10621 ccagtcattc agacgctggt gtagctcttt atcgtgagtc gcgagaattt gtgctgccag
    10681 taatgccgcg tttgccgcgc cagctttacc aatcgccagc gtacccaccg gaatgccgcg
    10741 cggcatttgt acgatggagt agaggctatc gacaccgctc agtgcggcgc tctgtactgg
    10801 cacgcccagc accggcacca gcgttttggc ggcaatcatg cctggcaggt gcgctgcgcc
    10861 gcctgcgccc gcaataatca cctgataacc gttctcttcg gcgctttcgg cgaagctgaa
    10921 cagtttatcg ggggtgcggt gagcagaaac cacttcaacg tggtgcggga cattcaggat
    10981 ttcgaagatt tcgacggcga actgcatggt agcccagtcg cttttggacc ccatcacgat
    11041 ggcgacacgc gccggattat tgcgggaaga catgcgtctt aaaactcctg tggtgcacaa
    11101 ctctcggctt tagagggcac agagaatagc acggaaagag agcaaggaaa acggttgcgt
    11161 ggctgtgaaa tcagcaaagt tgtgggtttt ttaaaacgga aaatgaatca gctcaacgtc
    11221 atccgccgtg actttcacca tcgaaccttc cgtatgccag gcacccagta ccacgcgaaa
    11281 agcaggttgc tggttggcga taagttcatg caccgccggg cgatgggtat gcccgtggat
    11341 cagccattgc acctgatgtt tttccatcgc actgaccaca gcgttttggt taacgtccat
    11401 gatcgccagc gatttactgc tgttggcttc tttgctgttc gcgcgcattc gcgcggcaat
    11461 gcgtttgcgc acaaacaacg gcagggcgag gaatagcatt tgcagccagg gtttgtggac
    11521 tttggcgcga aaagcctgat aacccgcgtc atcggtgcac agcgtgtcgc catgcataat
    11581 caacacccgg cgaccataaa gttcgagcac cttttcttcc ggcaataatg tcatgccgct
    11641 ttcacgggca aagcgtttgc cgagcagaaa atcacggttg ccatgaatga aataacaggg
    11701 aacgccagaa tcggacaccg ctttgatcgc tgccgccatc tggcgatgga gtgggttggg
    11761 atcgtcgtcg ccaatccatg cttcaaacag atcgccaaga atatacagcg cgtcggcctt
    11821 gcgggcttcc cccgctaaaa aacgcagaaa accggcggtg atcgccggtt cttccacgca
    11881 gagatgaaga tctgcaataa aaagtgtcgc cacgattact cgctaacggt cacgctttca
    11941 atgataacgt cttcttttgg cacgtcctgg tgcataccgt tacgaccggt tgcaacacct
    12001 ttgattttgt ctaccacgtc catgccgtca accacttcag caaacacgca gtagccccaa
    12061 ccttgcaggc tttcgccaga gaagttcagg aagtcgttat caaccacgtt gatgaagaac
    12121 tgtgcagttg cagagtgcgg agcctgagta cgtgccattg ccagcgtacc acgggtattt
    12181 ttcaggccgt tgttggcttc gtttttgatc ggttctttgg tggctttttg tttcatgccc
    12241 ggttcaaaac cgccgccctg aatcataaag ccgttgataa cacggtggaa aatggtgttg
    12301 ttgtaaaaac cttcgcggca gtagtccagg aagtttttaa ctgtttcagg tgctttatcg
    12361 tcaaaagttt tgatgacaat atcgccgtga ttggtgtgga aagtaaccat ttttgcatcc
    12421 tgttccgttt gattggtgct tcaacccagt tcgggtcata tatagggtgg tgttatagca
    12481 taaccgcacg atcggatcat cacgcaatgt atgctgattc gcgcgggaaa tatgggtatt
    12541 atacgcaact caattaccca cacatgtcta aacggaatct tcgatgctaa aaatcttcaa
    12601 tactctgaca cgccaaaaag aggaatttaa gcctattcac gccggggaag tcggcatgta
    12661 cgtgtgtgga atcaccgttt acgatctctg tcatatcggt cacgggcgta cctttgttgc
    12721 ttttgacgtg gttgcgcgct atctgcgttt cctcggctat aaactgaagt atgtgcgcaa
    12781 cattaccgat atcgacgaca aaatcatcaa acgcgccaat gaaaatggcg aaagctttgt
    12841 ggcgctggtg gatcgcatga tcgccgaaat gcacaaagat tttgatgctt tgaacattct
    12901 gcgcccggat atggagccgc gcgcgacgca ccatatcgca gaaattattg aactcactga
    12961 acaactgatc gccaaaggtc acgcttatgt ggcggacaac ggcgacgtga tgttcgacgt
    13021 cccgaccgat ccaacttatg gcgtgctgtc gcgtcaggat ctcgaccagc tgcaggctgg
    13081 cgcgcgcgtt gacgtggttg atgacaaacg caacccgatg gacttcgttc tgtggaagat
    13141 gtcgaaagag ggcgaaccga gctggccgtc tccgtggggc gcgggtcgtc ctggctggca
    13201 cattgaatgt tcggccatga actgcaagca gctgggtaac cactttgata tccacggcgg
    13261 cggttcagac ctgatgttcc cgcaccacga gaacgaaatc gcgcagtcca cctgtgccca
    13321 tgatggtcag tatgtgaact actggatgca ctccggaatg gtgatggttg accgcgagaa
    13381 gatgtccaaa tcgctgggta acttctttac cgtgcgcgat gtgctgaaat actacgacgc
    13441 ggaaaccgtg cgttacttcc tgatgtcggg ccactatcgc agccagctga actacagcga
    13501 agagaacctg aagcaggcgc gtgcggcgct ggagcgcctc tacactgcgc tgcgcggcac
    13561 agataaaacc gttgcgcctg ccggtggcga agcgtttgaa gcgcgcttta ttgaagcgat
    13621 ggacgacgat ttcaacaccc cggaagccta ttccgtgctg tttgatatgg cgcgtgaagt
    13681 gaaccgtctg aaagcagaag atatggcagc ggcgaatgca atggcatctc acctgcgtaa
    13741 actttccgct gtattgggcc tgctggagca agaaccggaa gcgttcctgc aaagcggcgc
    13801 gcaggcagac gacagcgaag tggctgagat tgaagcgtta attcaacagc gtctggatgc
    13861 ccgtaaagcg aaagactggg cggcggcaga tgcggcacgt gaccgtctta acgagatggg
    13921 gatcgtgctg gaagatggcc cgcaagggac cacctggcgt cgtaagtaat tgcgcaattg
    13981 ccggatgcga gttttcgcat ccggttatcg tctgcgccac cacaacattc ccatcagtag
    14041 catccccggc aaccacaccc acatcaattc ggaaataatc acctgatgcc cgtacgctgt
    14101 ggtgtaacga gacaatgcaa acggcgcgac tttaatcacc cgccagggag cgaaaaagcg
    14161 ttcatctgac cacggccaca gccagccaac gcccttaccg ccagtggtta ccgaatccag
    14221 taagctgtgc gatagcaacg agacggttaa aaacagccag cagcgaatca gcccagccct
    14281 gaaccatcga cgtccaatca gcacacataa gagcgggaca acaaacgcaa acaccagcga
    14341 atgggtaaac ccgcgatgac caaaaacatt gccgtaagca acgccaaatt taaacgacaa
    14401 tacgtcggca tcgggcagca tcgccaggat gattccggca aataacagac gcggagggat
    14461 gactttcgaa cccaacccta aaccaatgca aaggggaacg gcggcgtgcg taataacggt
    14521 tggcatgatg gtcgcttcgg caaaatgtcg atgctatcag catggatgaa cggggcgtag
    14581 agggcaaaag tctgaaaaga gaaccggcct gttgatacag gccgggaaag ggatcaggca
    14641 acaacctgta cgctgtgtcc tgcaaaactc actgtctgac cggcgacgat tttgcagcgt
    14701 ttgcgcgttt caaccgcacc gtcgactttc acctggcctt cggcaatcgc gattttcgcc
    14761 tgcgcgccgc tttcgctcca gccttccagt ttcagcaagt cgcacagctc aacgtgcgga
    14821 tgtttaccta aagaaaatgt cgccatgtta ctcaccctgt ggatcatgat attcaacgca
    14881 cgcctgtagc gtgttttcaa tcagcgtggc aaccgtcatc gggccaacgc cgccgggaac
    14941 aggcgtaatg tatgaggcgc gtttagccgc gtcttcaaac acgacgtcgc ccacaacttt
    15001 gccattttcc agacggttga tgccgacatc aatcacaatt gcgccttctt tgatccagtc
    15061 accgggaata aagcctggct tgccaacggc aacgatcaac agatcggcat tttctacgtg
    15121 atgacgcaga tttttagtga agcggtgagt cacggtggtg gtgcaacctg cgagcaacag
    15181 ttccatgctc attgggcgac cgacgatatt cgatgcgcca atcaccacgg cgttgaggcc
    15241 gaaggtatca atgttgtaac gctcaagcag cgtgacgata ccgcgcgggg tgcagggacg
    15301 cagacgcggc gcgcgctggc acagacgacc gacgttgtaa ggatggaaac cgtccacgtc
    15361 tttgtccgga tgaatacgtt ccagcacttt gacgttatca atacccgccg gtaacggcag
    15421 ttgaaccaga atgccatcga tggtgttgtc ggcattcagc gcatcgataa gctccagcag
    15481 ctccgcttcg ctggtggttt ccgggaggtc ataagagcgg gagacgaacc cgacttcttc
    15541 acaagccttg cgtttgcttg cgacataaat ttgcgatgca gggttactac ccaccagcac
    15601 aacggccagt cctggtgccc gcagtctggc tgcaatacga gcctgaactt tttgagcaac
    15661 ttcagagcgc acctgctgcg caatcgtttt accgtcaata atctttgctg ccatcagaga
    15721 gagaattcca tctgttacgt aggtcgaagg ggatgcgtct attttgtcag aagcggggcg
    15781 cgctgtcagg tttcgtttca gatttatcgc gtgaagcgac ctcttgcgaa ggttaggcgc
    15841 accgtcgctg aggtcgctga gattgaaagc ttcatttttc gtacatgatg gcgttgtaaa
    15901 tctggaactg atttatttcc ttgtttaagg attaag
//
LOCUS       DADKXM010000087        15924 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.088, whole genome shotgun sequence.
ACCESSION   DADKXM010000087 DADKXM010000000
VERSION     DADKXM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.088"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..179
                     /locus_tag="JQR05_002689"
     CDS             <1..179
                     /locus_tag="JQR05_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324829.1"
                     /translation="DEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDL
                     PRNCSGKIIRKNLK"
     gene            288..1073
                     /gene="caiD"
                     /locus_tag="JQR05_002690"
     CDS             288..1073
                     /gene="caiD"
                     /locus_tag="JQR05_002690"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY5324830.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            1079..1669
                     /gene="caiE"
                     /locus_tag="JQR05_002691"
     CDS             1079..1669
                     /gene="caiE"
                     /locus_tag="JQR05_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY5324831.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1755..2150)
                     /gene="caiF"
                     /locus_tag="JQR05_002692"
     CDS             complement(1755..2150)
                     /gene="caiF"
                     /locus_tag="JQR05_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY5324832.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(2412..5633)
                     /gene="carB"
                     /locus_tag="JQR05_002693"
     CDS             complement(2412..5633)
                     /gene="carB"
                     /locus_tag="JQR05_002693"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY5324833.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(5651..6799)
                     /gene="carA"
                     /locus_tag="JQR05_002694"
     CDS             complement(5651..6799)
                     /gene="carA"
                     /locus_tag="JQR05_002694"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY5324834.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFLGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(7254..8075)
                     /gene="dapB"
                     /locus_tag="JQR05_002695"
     CDS             complement(7254..8075)
                     /gene="dapB"
                     /locus_tag="JQR05_002695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY5324835.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(8242..9156)
                     /gene="rihC"
                     /locus_tag="JQR05_002696"
     CDS             complement(8242..9156)
                     /gene="rihC"
                     /locus_tag="JQR05_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY5324836.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(9222..10172)
                     /gene="ispH"
                     /locus_tag="JQR05_002697"
                     /gene_synonym="lytB"
     CDS             complement(9222..10172)
                     /gene="ispH"
                     /locus_tag="JQR05_002697"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY5324837.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(10174..10623)
                     /gene="fkpB"
                     /locus_tag="JQR05_002698"
     CDS             complement(10174..10623)
                     /gene="fkpB"
                     /locus_tag="JQR05_002698"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5324838.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(10710..11204)
                     /gene="lspA"
                     /locus_tag="JQR05_002699"
     CDS             complement(10710..11204)
                     /gene="lspA"
                     /locus_tag="JQR05_002699"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY5324839.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(11204..14020)
                     /gene="ileS"
                     /locus_tag="JQR05_002700"
     CDS             complement(11204..14020)
                     /gene="ileS"
                     /locus_tag="JQR05_002700"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY5324840.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYVEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(14063..15004)
                     /gene="ribF"
                     /locus_tag="JQR05_002701"
     CDS             complement(14063..15004)
                     /gene="ribF"
                     /locus_tag="JQR05_002701"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY5324841.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(15012..15230)
                     /locus_tag="JQR05_002702"
     CDS             complement(15012..15230)
                     /locus_tag="JQR05_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY5324842.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SYFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            15333..15596
                     /gene="rpsT"
                     /locus_tag="JQR05_002703"
     CDS             15333..15596
                     /gene="rpsT"
                     /locus_tag="JQR05_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY5324843.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3811 a         4352 c         4019 g         3742 t
ORIGIN      
        1 gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg
       61 aattttttcg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga
      121 tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa
      181 tgaaacggca gggaactacc ctgccagcta ataatcacgc cctaaaacaa tatgcatttt
      241 ttgcagggat gttgtcgtcc ctaaaaaagc aaaaatggag aaaaggaatg agtgaatcat
      301 tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgccca aaagcgaatg
      361 ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc
      421 cgcaattacg tgtcgccatt attaccggtg ccggagagaa attcttttcc gcgggctggg
      481 atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggttttg
      541 cgggattaac cgaaattttc aatctcgaca aaccagttat cgcagctgtg aacggctacg
      601 cctttggcgg cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg
      661 ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc tgacagcggc ggtgtgctgc
      721 gtctgccgaa gatcctgccg cctaccatcg tcaatgaaat ggtgatgacc ggcagacgaa
      781 tgggcgcaga agaggcactg cgttggggga tagtcaaccg cgtggttagc caggcggaac
      841 tgatggataa cgcccgcgaa ctggctcagc agctggttaa cagcgccccg ctggcgattg
      901 cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct
      961 atattcgcag cggcgtgttg aaacactatc catcggttct gcattcggaa gatgccattg
     1021 aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt
     1081 gagctattac gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc
     1141 cagtgccgtc ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc
     1201 ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg
     1261 ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg
     1321 gcatggagcg atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag
     1381 cgtaattatg gatggcgcgg tcattggcga agagagcatt gttgccgcaa tgagctttgt
     1441 caaagcaggc tttagtggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt
     1501 acgcagtgtt agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga
     1561 tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag ccactgaggc aaatggagga
     1621 aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat
     1681 gcgacctggt tgctggataa gatgctacaa catcgtatct ggcaagcagg acgcccggta
     1741 tcaacgagat aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg
     1801 cggttaagtt ccgtgagcgg aacggcagga atacgcggtg tctttcttac gccgactaat
     1861 ttttcccgac tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccacttta
     1921 accagtcgct gacactggca tccccgccct tccagcttat tagggatcat cttaacttcg
     1981 cagcttattt ctgtgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt
     2041 tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc
     2101 cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca
     2161 gtttcacaaa ttaaaagtct ggctatgact cgttttgaag caattaaaca aggccatatt
     2221 aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct
     2281 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaaacg
     2341 gtcctcatca gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc
     2401 acgacacgct attatttgat ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc
     2461 gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc
     2521 actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct
     2581 gaggtggtgt tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga
     2641 cggccttcat gcaccttgtt taccagacgc ggattgatac ccgcttcgcc cagcacaatc
     2701 gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc
     2761 accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg
     2821 gtggagttgc tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc
     2881 atgacttccc cggtagagcg catttctggc cctaacagcg ggtcaacgcc cgggaattta
     2941 ttgaacggca gcaccacttc tttcaccgag tagtacggcg gaataacttc tttggttacg
     3001 ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg
     3061 ccggtggctt tggagacgaa cggaacagta cgcgccgcac gcgggtttac ttcaatcagg
     3121 taaacttcgt tgtttttcac cgcaaactgc acgttcatca gaccgcgcac ctgcaattcg
     3181 aaagccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtac
     3241 gctggcagcg aacatgcgga gtcaccggag tgcacgcccg cctgctcgat gtgctccatg
     3301 atgccgccaa tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg
     3361 tcatcaagga aatgatccag caacactggc gcatcgttag acacgctaac cgccgtctgg
     3421 aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg
     3481 taagacggac gcaccaccag cgggtagcca atctcttttg ccttctcaac tgccatttca
     3541 atagcagtaa cggtggcgtt cgccggttgt ttcagtttca gacggtcaac cgcatgctgg
     3601 aagcgttcac ggtcttccgc acggtcgata gcatccgggc tggtgccgat aaccggtacg
     3661 ccagcggctt ccagtgcacg cgccagtttc agcggggtct gaccgccgta ctgaacgata
     3721 acgcctttcg gcttctcaat acgcacgatt tccagcacat cttccagagt taccggctcg
     3781 aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg tttccgggtt acagttaacc
     3841 ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg
     3901 aattcgatac cctgaccgat acggtttgga ccgccgccaa gcaccatgat tttttcacgg
     3961 tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta agcggtgtcg
     4021 gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg
     4081 tcacgcagct tacggatttc cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg
     4141 gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt gatgcccact
     4201 tccgccactt tctcttccag acgcaccagt tcttcaatct gtaccaggaa ccagcggtca
     4261 atgttagtca ggttgaagac gccgtccaca gacaggcccg cgcggaaagc atcggcgatg
     4321 taccagatac gctctgcgcc tgcgtctttc agttcgcgac ggattttggt taacgcttcc
     4381 gggtcatcta ggctcacttt cgggtcgaag ccagttgcgc ccacttccag gccgcgcagc
     4441 gctttttgca gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc
     4501 atctgagtgg tcagacggtc gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt
     4561 ttagtaacca catagtcgat ggacggctcg aaggaggccg gagtacgtcc gccagtgatg
     4621 tcgttcatca gttcgtcgag ggtgtaaccc accgccagtt tcgccgccac tttagcaatc
     4681 gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg
     4741 ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacattgga accaccggtt
     4801 tcaacgccga tttcacgcag caccgccatc gaggcgttac gcatgatttg atattctttg
     4861 tcggtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat gcccatcgca
     4921 tcaaagtttt cgatagagca gacgatgatg cagttgtcgt ttttatcacg caccacttcc
     4981 atctcgtact ctttccagcc gatcagcgac tcatcaatca gcaactcttt ggtcggagag
     5041 agatccagac cgcgggcgca aatttcttca aactcttcac ggttataagc gataccgccg
     5101 ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagcccac gtcagcggca
     5161 acagccagcg cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt
     5221 ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc atcggcagtg
     5281 gcaccaatca tggtgacacc gaactcttcc aacacgccct gacgttccag ctccagcgcg
     5341 cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg cgtccgggcg ctctttttca
     5401 ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc
     5461 gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta accctcttcg
     5521 cgcagggctt tacatgcttg cgcgccagag tagtcaaact cacacgcctg accgataaca
     5581 atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg catggctctt
     5641 ttactcctga ttacttagcg gttttacggt actgctcaat taacttgata aagtggtcga
     5701 acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg gtgaccctgg aagctgaacg
     5761 ccggtttatc gatgcgatga atgccctgta acgtaccgtc gaacagggat ttatgcgtga
     5821 cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc tgggcggtga
     5881 tcatcaccac gtttttctcc acatctttaa ccggatggtt gccgccgtgg tgaccaaatt
     5941 tcattttgac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg agacagatgc
     6001 cgaataccgg aatatcggtt tcgaggaact tctggatggc ggtaatggcg taatcgcacg
     6061 gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt ttcagcacat
     6121 cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc accagcatcc
     6181 gcaggatgtt gcgcttggca ccaaaatcat aagccacgac gtggaacggc agctcgtctt
     6241 cttttttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc cagctatagg
     6301 cttctgcggt ggtcacttct tttgccagat ccatgccgtt cagacctagg aacgcgcggg
     6361 ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc gataatgcag ccattctgtg
     6421 cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca atcgccacga
     6481 tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg ctggcaatca
     6541 gcggcaggtc gcgaatcacc agaccttgtg catgtacctg agaagattct tcatcggcgt
     6601 cattagtgcc gacattgcca atatggggat aagtaagagt aacgatttga cgagaatagg
     6661 aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg acttccccaa
     6721 ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct tccagaacca
     6781 atagcgctga cttaatcaaa acaccctcca gagaatattc actcacttta tttgcatatt
     6841 aattcacagt gatgacgtaa atcaatgcaa atctgcttac cgacgaattt ctggcaaacg
     6901 gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcaggattt tttatattat
     6961 tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aacatcatct aacctgttct
     7021 ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa acgagcagga aaaagtggac
     7081 caaatggtca aaatttacat taaaacaaca aaaatagaag acgaaacatg taattttggc
     7141 agttatagaa aatttaaaag cataacacca aaaataaaag ggctaaaaat taaagccctt
     7201 tataattaat aaattaaatg tgttactttt gcatcataat aaacattttg tggttacaaa
     7261 ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc actcaaccac
     7321 aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt atgggtgatc
     7381 tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca
     7441 cgcacggtgg caaaaccaat ggtaccaggc acacgttcac cggtatggcc ttcacgactg
     7501 tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc tcccattgcc
     7561 agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat aatttcgata
     7621 tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat gacgttaacg
     7681 ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc
     7741 tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc atgctgccga
     7801 caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa
     7861 tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc tgcaccagcc
     7921 agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc
     7981 tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg ccccccggct
     8041 cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg ttaatttgca
     8101 tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac gctgataatt
     8161 agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat gaatgactgc
     8221 atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca cccactgttg
     8281 gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc ccaggcaacc
     8341 gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa
     8401 acacggcttg agggtgaata agtccgggcg caccagccag gcgatggcgc agagatcgtg
     8461 cattcgcaga ccgctttgca tactgccgct acggtagtgg ctaaacaggg cgtggagcat
     8521 ttttccggtg cgattcaact ccggtaaagt agcgagatag tcaggggtta atattgcctg
     8581 attggtgaca tccagaccgc acatgacaat ttcgatgccg ctacggaaga cacaggcagc
     8641 cgcttctgga tcggcagcga tattaaactc ggcatttggt gtacagttgc cgcgaccagc
     8701 agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc attgtaaaag
     8761 taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct ctggtgcacg
     8821 catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc ggttgtgctc
     8881 aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc
     8941 gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc agaaatgcag
     9001 cagttgcagg gcattgcggg tagttttctc aaccgagaca ttacccgcga cggtggtcat
     9061 cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg catcgtcaat
     9121 gccggggtca gtatcgagga agataggtaa acgcatgttt tcttcataaa aaatgccggt
     9181 aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac ttcacgaata
     9241 tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc ttccagcgga
     9301 atggcttcac caccacccaa ctgctgcaaa cgtgccacca cattctgcac cagaatatcc
     9361 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctgg
     9421 atatctttcg catcgtcaat caaaaacgcg tgtttgccca tacgctgggc cagctccgcc
     9481 agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc cgcctgttct
     9541 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
     9601 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
     9661 accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc
     9721 cacacatcgt ctggcgattc gaccagatac attccccctt cggggttgct gtactgcccc
     9781 atcgtccctt ccacttccgg gtgcccggcg tggccgatga gaatagattc ttcgccacgg
     9841 cgactggcgc gggcaacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
     9901 accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag
     9961 aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct caataaagat agccccacgc
    10021 tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
    10081 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
    10141 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
    10201 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
    10261 aatggagtcg ccgttaattt cgcggatcac accaggcatc tcactgccat ccattgcggt
    10321 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
    10381 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
    10441 ggttttgtcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
    10501 atcgcccagg cggaacagcg ccggtttacc gttgttgcga gtagactcgg cggtggtgcc
    10561 atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
    10621 catgcaggtt tgctcttatt tttgaggatt tttgccggat gcggcctacg atttcgtagc
    10681 aatctgtagg ccgcatccgg cagggtttat tattgttttt tcgctttgga aggtaaaaac
    10741 ccttccagca cgatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    10801 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    10861 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cgtaagcgat attgtttagc
    10921 ttctgcgtgg ccttcgagcg atacatcatc actgccagga gcacgctaat accaatcgca
    10981 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    11041 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccaga
    11101 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    11161 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    11221 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    11281 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    11341 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    11401 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    11461 cggtcagttt cgccgccagt tccggttcta catacaaggt tactgccgct tccagcgagc
    11521 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    11581 tcaacagctc gtcccagaac gcatcgttca ttgcttcact gtctgccaga ccaaacaggc
    11641 cttcgtacca ctcaccggtg aagacgtatt tttcacgttc gcccggcaga tagccccaca
    11701 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tcagcgatgt
    11761 gatacagcgc agtctggcag ctacgacgcg ccacgctatc cgctttggcg gtgtactgac
    11821 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    11881 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    11941 ctttcgcaca acctacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    12001 cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    12061 gacgatagct atcggcagca cgtttcagga tctcgtcaga aacggccatt tcaccggtgt
    12121 agtcggttga tgccacccac agacgcagaa tatccgcacc cagtttgttc atcacatcct
    12181 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    12241 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatta
    12301 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    12361 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    12421 atacgtcgag agtatccggc actttcacat actggtcagc ttcgtcgccg aggatctctt
    12481 tcgcatcgag atcccaccac gcctggatgc cgtcaacttc aacgcgttta gccacttctt
    12541 ccatcagttc gagggtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    12601 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    12661 actcgatacg cgcctggccc cagtccggga tccactgcac gcctttgatc tctttcagtg
    12721 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga
    12781 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    12841 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    12901 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    12961 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    13021 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    13081 cgaagcccat aaacggatgg gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc
    13141 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    13201 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    13261 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    13321 ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc atcctgatcg accgcctgga
    13381 aagcgacgtc aatggacgga gaagttttgt cgtaatactc aacttccgct tctgccagtg
    13441 cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc
    13501 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atagtcagat
    13561 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    13621 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    13681 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    13741 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    13801 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    13861 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    13921 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    13981 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    14041 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    14101 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    14161 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    14221 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccgatgt
    14281 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    14341 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    14401 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    14461 ctgccagagc cagattgtca tcagccagcg cctgacgcac ggcggtgctg ctgatacgca
    14521 caccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa gccgtattcc atgccagctt
    14581 tctgtaataa caagaaatcg ccttcacgac cagcgccaaa gcggaaatca tcacctacgg
    14641 caagaaattt tacgcgcaaa tgcttcacca aaagatcgct gatgaaattt tgcgcggtta
    14701 acgccgcaaa acgcctgtcg aaacgcacgc acagcacgta atcaacgcca cactctgcaa
    14761 ggtaacgcag tttttcccgc agccgggtca gtcttgccgg ggctttatcg gtagcaaaca
    14821 gttccagtgg ttgaggttca aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt
    14881 cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa
    14941 tagtcagcac acacccttct tgcggggcct ggctgagatt atgtatgccg cgtatcagct
    15001 tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt
    15061 aaccggcgat tgagtaccga aatcagaaag aagtagcttt tcattgccat ggcgcaaatc
    15121 acgggaagaa actgaccgcc tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc
    15181 gcaagctggt agaatcctgc gccatcacta cgtaacgagt gccggcacat taacggcgct
    15241 tatttgcaca aatccattga caaaagaagg ctaaaagggc atattcctcg gcctttgaat
    15301 tgtccatata gaacacattt gggagttgga ccttggctaa tatcaaatca gctaagaagc
    15361 gcgccattca gtctgaaaag gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta
    15421 ctttcatcaa gaaagtatac gcagctatcg aagctggcga caaagctgct gcacagaaag
    15481 catttaacga aatgcaaccg atcgtggacc gtcaggctgc taaaggtctg atccacaaaa
    15541 acaaagctgc acgtcataag gctaacctga ctgcacagat caacaaactg gcttaatcgc
    15601 caatttgctg aagctttgtg aaaaagcccg cgcaagcggg tttttttatg cctgctgctt
    15661 ttgtgatgcg tgagagaaca cactgccggt tgccagatag tcaaaagaga catgccgttg
    15721 tactatctga actcttcgtg gcttaagaat cgtagaacca tcggctgata cattctgaac
    15781 aagaatatct actcaatctc cagactcccc cacaaagaat atggatattg tgatacacat
    15841 tgaggtatta aacttaagac cactctattt ttgaattaaa ttcctatata ctttaaccat
    15901 tcataaatat ctcctcatta gacc
//
LOCUS       DADKXM010000088        15895 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.089, whole genome shotgun sequence.
ACCESSION   DADKXM010000088 DADKXM010000000
VERSION     DADKXM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.089"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..63)
                     /locus_tag="JQR05_002704"
     CDS             complement(<1..63)
                     /locus_tag="JQR05_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5324844.1"
                     /translation="MNKKLMYIFAIFIVAAITCIS"
     gene            165..872
                     /locus_tag="JQR05_002705"
                     /pseudo
     CDS             165..872
                     /locus_tag="JQR05_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            869..2320
                     /gene="djlC"
                     /locus_tag="JQR05_002706"
                     /pseudo
     CDS             869..2320
                     /gene="djlC"
                     /locus_tag="JQR05_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(2380..4050)
                     /locus_tag="JQR05_002707"
     CDS             complement(2380..4050)
                     /locus_tag="JQR05_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY5324845.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4134..5069)
                     /gene="rihA"
                     /locus_tag="JQR05_002708"
     CDS             complement(4134..5069)
                     /gene="rihA"
                     /locus_tag="JQR05_002708"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY5324846.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(5187..5912)
                     /gene="gltL"
                     /locus_tag="JQR05_002709"
     CDS             complement(5187..5912)
                     /gene="gltL"
                     /locus_tag="JQR05_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY5324847.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5912..6586)
                     /gene="gltK"
                     /locus_tag="JQR05_002710"
     CDS             complement(5912..6586)
                     /gene="gltK"
                     /locus_tag="JQR05_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY5324848.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6586..7326)
                     /gene="gltJ"
                     /locus_tag="JQR05_002711"
     CDS             complement(6586..7326)
                     /gene="gltJ"
                     /locus_tag="JQR05_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY5324849.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7496..8404)
                     /gene="gltI"
                     /locus_tag="JQR05_002712"
     CDS             complement(7496..8404)
                     /gene="gltI"
                     /locus_tag="JQR05_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY5324850.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8801..10339)
                     /gene="lnt"
                     /locus_tag="JQR05_002713"
     CDS             complement(8801..10339)
                     /gene="lnt"
                     /locus_tag="JQR05_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY5324851.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10364..11242)
                     /gene="corC"
                     /locus_tag="JQR05_002714"
     CDS             complement(10364..11242)
                     /gene="corC"
                     /locus_tag="JQR05_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY5324852.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQKLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11332..11799)
                     /gene="ybeY"
                     /locus_tag="JQR05_002715"
     CDS             complement(11332..11799)
                     /gene="ybeY"
                     /locus_tag="JQR05_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY5324853.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11796..12875)
                     /locus_tag="JQR05_002716"
     CDS             complement(11796..12875)
                     /locus_tag="JQR05_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY5324854.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPKYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            complement(12989..14413)
                     /gene="miaB"
                     /locus_tag="JQR05_002717"
     CDS             complement(12989..14413)
                     /gene="miaB"
                     /locus_tag="JQR05_002717"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY5324855.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14559..15734
                     /gene="ubiF"
                     /locus_tag="JQR05_002718"
     CDS             14559..15734
                     /gene="ubiF"
                     /locus_tag="JQR05_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY5324856.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVIPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3878 a         4039 c         4105 g         3873 t
ORIGIN      
        1 gctaatacag gtaattgctg caactataaa aattgcgaat atatacatta attttttatt
       61 catgtgtgaa tgcttcgatt ttctttttat tttgaaagcg tcaagtttat actcatcaat
      121 aataaaaaag tagtcagtta atgcacttaa gggattatta aaaaatgaat aaggaagaac
      181 aatatcttct ttttgcgctt tctgcgccca tggaaatact taatcagagt tgcaaacccg
      241 cgcatgactc gccaaaaatg tataccggga taaaagaatt cgatctctca tcatcctggg
      301 gaataaataa tcgtgatgat ttaattcaga ctatttatca gatgactgat gatggtcatg
      361 ccaatgattt agctggctta tatctgacat ggcaacgctc ttcccctgaa gagtggaaag
      421 cattaattgc tggtggctcg gagagagggt tgatttatac ccaattcgtt gcccaaaccg
      481 ctatgtgctg tggagaaggg ggaatcaaag cctgggatta tgttcgaatg gggtttctgt
      541 cacgggtcgg tgtgctcaac aactggctga cggaagaaga gagtttatga ctgcaatcgc
      601 gcgtctatgc acgagctcac cattactatc acagctggat gcactatttt tctgcttatt
      661 cactagggcg tctctactgg caatcttctc agtgcgaaga caacgcttcg ctacgcgagg
      721 cgctaaccct gtacaaatac gatagcgccg ggagtcggat gttcgaagag ctggcagcgg
      781 gaagcgaccg tttctatgcc acgcttccct ggcagccatt aactgtccag cctgaatgcc
      841 cggtaacgct taaggatgtg agcgacctat gaaaacatgt tggcaaatcc tcgaaattga
      901 aagcacgacg caaatagaca ttatccgcca ggcttatctt gctcgcttac cgttgtgtca
      961 tcccgaaacc gatccgcagg ggtttaaagc attacgccag gcctatgaag aggcgctgcg
     1021 actggcggta aatcctgtcg aggaagcaga tgatgaagaa aaagatgcca ccgctgaaca
     1081 tgaaatacta cgtgcattca ggacattact ggattcagaa agtgatcgtt ttcagccctc
     1141 cgcctggcag aaatttattc agcaattaaa tacctggaac atggaggatg tcgatcaatt
     1201 acgctggccg ctgtgtgcaa tcgctataga agcgcgatat ctgtcattaa attgtgcttc
     1261 tttgttggca gagcgtttga actggcattc gtttaatgac agcgaaggaa tggatgagga
     1321 agaaagggag gcttttcttg aggccattca ggctggtgat tgtttcgatt tccttagcct
     1381 tctggaatat cccgtcgcgt tacagaacca gactgttgaa tattacttcg cgctggaacg
     1441 ttgctgccgt taccatcctg actatgtcac tgcgtttttg gcgatggaag gaccgtggtt
     1501 cattcctgat gatgcaaagt tacatcgcaa actgttgcgc tggtacagct cggtgcaaac
     1561 aggtatggtg gaactcattc ctgtagccaa acagtggcaa gcggaagaac cagaaagcga
     1621 agatgcccgg tattactagt gtgcacaacg tttgtactgc ggcgaggggg aaagcctgct
     1681 tgccgatctc ggcgcgtact gggaaagtta cccatctaca caagctgata atttgttgtt
     1741 gcagtggagc aagcgtcatt gcccggatta tttcgcgtta ttagtgatgg ttatcgaagc
     1801 gcggagcatg gtagatgcgc aaggtcaacc gctgaaatat gttcctggtg agagcgcccg
     1861 gacgcggctg ttatgggcgg aaattttaca tagcggaaaa ttatcgccgt taggtcaatc
     1921 gtttattgag tcgttattct tcaaacgcaa agcatgggcg tggtggaaat cgagagtcgg
     1981 tagcgagaca gaagaagatt caccgttact ggatttgtat cgggtagcgg aacaggtagt
     2041 acttgaagcg tttccgaaac aagagatgct ggcccgtctt aatacaaggc tggaaggcgg
     2101 agatgctcat ccattagagg ccattatcac ccggatgctg ttgacgaaag taaaactcga
     2161 gccggaggat gaagatgtcg atgagccaac acctgaaaac catgaagaaa aaaatgatga
     2221 gggtgaaaaa ccacagagca ttaccagcat tatcaaaatc agtttaacgg tgctggttat
     2281 aggttatgct ctcggcaaaa tcgcgatgtt gtttagttga cgatgtgttt tttgcgccga
     2341 tgccggatgc agcatttacg ccgcatccgg cagccgtgat tatgaggtct caatggctaa
     2401 ataaacgctc gcccgccgcc tgacctcatc gacgcgggcc atatcgtttg actgcagcgc
     2461 cgcatcaaaa tcggtgatca cccggccaat ctcttctcgc tcgtcaccca gcgcccgcgc
     2521 ccacaactct tccagtttgg ctttaaaggt gcgattgatg agcatatcgc gcggataaat
     2581 tttcaatgcg gataaccgcg tccgactctc ttcaatctgc tgcgcgctca atgttaccgg
     2641 gctgtggtta atcactctgg acttaacgct gccgtcttcc agaagcacgt caacttcaag
     2701 caacccgttg atatcataac taaagcgaat atcaatcgac tgataagccc cggttttctt
     2761 caacggcaca tcgaaggatt ccaccagaat gttgttttta actttgtggt tttctccctg
     2821 atagacgtta accgtaattg aatcctgttc cgggtgcatg gttgaataag tttctacacg
     2881 cgacacgggc acagtggtgt ttcgttcaat aatcggcgag aaaatgccgg aaacgccctg
     2941 gcggttaacc tcaacgccca acgagtaagg gcaaatatca gtgaggatca cttcttcaat
     3001 atcttcactg cgtaagcggc aggcggcctg gattgctgcg cccagcgcga caatggtgct
     3061 cggatcgtaa ctttgatacg gtaatttgcc aaacagacgc acggcgattc gctgcaccag
     3121 cggcatttgt gacgcgccgc caaccagcac cagactgtcg atttgactcg gcttcagacg
     3181 cgcatcgcgc aacgcctgtt caatcggcac tcgcaagcga ttgagcagcg gcaaccacaa
     3241 atcttccagt tcgttctcgt aaaattcgca ttcccgcgtt tcatcctggt actgccagcg
     3301 aatgtgcatt ggcgattgat tgctacattt tgccgcttcc acacaggcgt acaaggctgc
     3361 cagttcactc tcgttaagcg tggtccttgt gacatccgcg cgctttaaaa cgtcatcgac
     3421 caacatatgg gtaaaatctt cgccaccgag gaagttgtcg ccagcggagg cgtgaacttc
     3481 aatcaccggc gtggcgtact caagcaccgt aacgtcaaac gtgccgccac cgagatcaaa
     3541 caccagcgaa cgggtatttt gttgggtatg caggccatac gccatcgcag ctgctgtggg
     3601 ttcattaatt aagcgtaccg catttaaccc ggctaagtcc gctgctaaac gggtatgctt
     3661 gcgttgttca tcgctgaaat aagccggaac ggagatcacc acatctttaa tcggacgttg
     3721 cagaaattct tcggcatctt cttttaatga gcgtaatacc aaagaggaca gttctggcgc
     3781 gttaaatgtg tcgctgccta accgccagtt ggtattactg cccattgcac gtttaaataa
     3841 cgctgccgtt ttatccggat gcgaagtacg ccgtgataca gccggttttc cgactaaaat
     3901 atgattattt tcatccatgc taattatgga tggtgttaaa tattcaccga acttatttgg
     3961 aattaattgc gcggcaccgt ctttccagac ggcaattaaa ctattggtag tgccgagatc
     4021 gatacctatg gcgagttctg cattatccat tgcatttata tcttgaaaga aaaagagata
     4081 aatagattaa gaccacccag gtaacgcgtg gtgatcttaa tcaatgacga gtgttaagcg
     4141 taaaatttca gacgatcagc cagtaaatca acaaagccct gacgatcaac atcgacaatt
     4201 acagtggcat tcggtttatt gccagtcaga taataataat caacaaccgt cataccctgg
     4261 gtatatttcc cctgtgtttc cacgccaacc cagcgctcaa cagtggtaaa caactcgggt
     4321 ttcaacagcc aggcgatggt gcatgggtca tgcagcggtg cgccgacaaa gccccatttt
     4381 tcgtctttat gatattcgag gaagaaatcc agcagttcgg caacaatggt tgaaacaggg
     4441 ttgccaatcg cgcggaaacg ctcggtgtct tcaacgtgga tttgcgcttt atgagtaaca
     4501 tccagaccgg ccatcaccac cgggatccct gactggaaga caatttctgc cgcttccggg
     4561 tcaacgtaaa tattaaattc agccgcaggc gtccagttac caagccccat tgcgccgccc
     4621 ataatcacga tacgggcaat tttgctatgc agttccggat ggctattgag cagcaaggca
     4681 acgttagttt gcggtccggt agaaacaatc gtgacaggtt ccgcactttc gcgcagcgtt
     4741 ttcgccatca gctccaccgc cgtacagttt tgcggtgcga acgtcggttc cggtaatgcc
     4801 gggccgtcga gaccgctttc gccgtgcaca ttgtccgcga taatcaactc acgcattaac
     4861 ggttttaccg cgccgcctgc caccggaata tcggtgcgat taagcaaggt cagcatacgc
     4921 agaacattgc gtaaggtttt ttctggtgtc tggtttccgg cggaagacgt aattgctttg
     4981 acatcaagct ctggtgaggc gagggcgaga actattgcga tagcgtcatc atgacctggg
     5041 tcgcaatcta acagaattgg cagtgccatt gttgctcctt gttgtgtgct tctttgcgac
     5101 aagggtaacg ccaggatgta acagatacga ggggcgaaac gataaagcgt gagatggcgc
     5161 gcaattgggt atgcgcgcca gagtgattaa tgcaggattt tcgcgaggaa gtcttttgcg
     5221 cggtccgatt tcggatcatc gaagaaagcg tctttcggcg agtcttcgac aattttaccc
     5281 tcgtccataa agatcacccg attcgccact ttgcgggcaa agcccatttc gtgggtcacc
     5341 accatcatgg tcattccttc gttcgccagt tccaccatca cgtccagcac ttcgttgatc
     5401 atctccggat ccagcgccga tgtcggttcg tcgaacagca tcgcaatagg atccatacac
     5461 aatgcgcgag cgattgccac acgctgctgc tgaccgccgg aaagctgcgc cggaaattta
     5521 ttggcatgtg cggaaagtcc cacgcgctcc agcaatttca atgctttttc acgagcgggc
     5581 gctttatcgc gtttaagcac tttcacctgc gccagggtca ggttttcgat aatcgacaga
     5641 tgagggaaca gctcgaaatg ctggaatacc atcccgacgc gggaacgcag ctttgccaga
     5701 tcggttttct tgtcgttaac cacgatacca tcgacggtga tttcaccttg ctgcactggt
     5761 tcgaggccgt tgacggtttt aatcagcgtt gatttgccgg aaccagacgg gccgcaaacc
     5821 accaccactt cgcctttttt cacttcggtt gagcagtcgg tcagcacctg aaagtgacca
     5881 taccattttg aaacattttt cagggtaatc attatgctgt ccttcttttc aagtagctga
     5941 ccaacaacga cgcgctaagg ctaataacga aataaacaaa tccggcaaac aggatcatct
     6001 cgacctgcgt accatcacgc tcaccaatgg ttgaggcggt acggaagaaa tcggccagag
     6061 ataacacata caccagcgag gtatcctgga acagtacgat gccctgagtg agcagcagcg
     6121 gcaccatcgc gcggaaagcc tgcggcagaa taatcagttt catcgactgc cagtgagtca
     6181 tccccaacgc cagcgcggcg ctcgactgac cacgggaaat actttgaata ccggcacgga
     6241 taatctctga atagtaggcc gcttcaaaca tcgaaaacgc caccatcgcc gaaattaaac
     6301 gaatatcatt ttttggcgat aatcccagca cattttgcag aaatcccggc acaatcaagt
     6361 aaaaccacag caaaaccatg actaaaggaa tcgagcggaa tacgttaacg taggctttgg
     6421 caaaccacga aacaggcgcg aagctggaca agcgcatcac cgccagcatg gtaccccaca
     6481 aaataccaat cacgactgcc gtgacggtga ttttcagggt gatcaccagc ccgtcgagca
     6541 gatatggcag ggaaggaaca atggaactcc agtcaaactc gtacattatt tgccccccat
     6601 gtttccgggc aggcgaactt tacgttcaac cagcgtcatc accagcatga taaaagcgtt
     6661 aatcaacaca tacgcaagcg tgatggcggt aaacgactcc caggcgtgag ccgagtagtc
     6721 gagcaattta cccgcctgcg ccgccatatc caccagaccg atggtcgagg cgatggcgga
     6781 gtttttcacc aggttcatca tctcggaagt catcggcggg acgataacgc gataagcatt
     6841 aggcagcagt acgtatcgat aagtctgcgg tagcgtcagg cccatcgcca gcgcggcatt
     6901 cttctgccca cgcggtagcg actgaatagc ggcgcgtacc tgttcgcaaa cacgggcggc
     6961 ggtaaacagc cccaggcaga gcatggatga aaggaaaaac tgaatattgg gatccagctc
     7021 ggccttaaac cacatgccga ttttctccgg cagcagctcc gggatcacca gataccaggt
     7081 aaagaattgc acaatcaacg gcacgttgcg gaacagttcg acatacaacg tacccagacc
     7141 ggagagaaaa cggtttggaa cggtacgtaa aatgccgaaa aatgaaccga cgaggaaagc
     7201 gataatccag gcgcagatcg acaaagcgat cgtcacctga aaaccactcc agacccaacc
     7261 aagataggtg gtattgccga acggggcttg ttgtaaaaaa ataccccagt tccagtctat
     7321 agacataaat ctactccaga aaaaagaggg tagcagcgtt aactgctacc ctcgaagatt
     7381 gttacccagc gtattgcggt tttcaggccc gatggggaac gaccatcagg cgtatagtct
     7441 gtccgtgcta cgtaacaatc gagagggctg gaatttccgc ccctggttct tgtaattagt
     7501 tcagtgcctt gtcattcggt tctttgaaca gtgctttcat ttcgtctgac agttcgaaat
     7561 tcatgttcag gtttttcggc ggaattggat ttttgaacca tttatcaaac catttttccg
     7621 cttcaccgga ggtctgcacc tgagcgatag tgtcatccat cagctttttg aactgcggat
     7681 cgtctttacg caacatgcaa ccgtaggctt cctgagactg cggcttgccg acgatatccc
     7741 agttgtctgg tttcttcgct ttcgcacgtt caccggccag cagagcgtca tccatcataa
     7801 aggcaacggc acgaccgctt tccagggtgc ggaaagagtc accgtgatct ttggcgctga
     7861 tgatgcgcat attcattttt tgctcttcat tcagtttgtt gagcaaaact tcagaggtag
     7921 tgccggaagt gacgactacg gctttgcctt tcaggtcggc aaaatctttg atatcgccgc
     7981 cctttttggt caacaggcgc gtaccgacca cgaaaatagt gtcagagaaa gccgcctgtt
     8041 tttggcgttc gacgttgttg gtggtagaac cacattcaaa atcgaaagtg ccgttttgca
     8101 gcagtggaat acggttttgt gaggtaatcg gaatcagttt tacctgcaag tccggtttgt
     8161 tgagtttctt tttcactgct tcaacaatgg cgttggagta atcctgcgag taacccacca
     8221 ctttttgctg attgtcgtaa taagagaaag gtactgaaga ttcacggtga ccgacgacaa
     8281 tcacaccgtt tttggcgatt ttgtccagcg tactgcctgc tgccggggcg gcgtcatctg
     8341 cctgtgccag tcctgcggaa agcgccaggg cgaggattgc tgtggcaggt ttacgtaatt
     8401 gcatatccaa ctcctttatc ttctgcgtta agaacgcatg gatacccgtt gtgagtgttt
     8461 gtgttgttat cgtctgcaac tttattgtgc agtgttgtgc ctgttaggta acatttagtt
     8521 tggctaaatg taaagatatt gatgttttat tgtttgtttt tgcgaggtgc accgcaccat
     8581 tctgaagcaa aattcttaaa atgcactctt ttggtgctac cggtggattg ctgtggtgca
     8641 accgggttgt actgatgctg gttcaagctt tgccttgtat aacaaagcaa tagatgtgcc
     8701 aaagttggat aggagaatat tgttatccgg ataatgcact gatgccgcat ccggtgagcg
     8761 tgccgaaata tgggatgtat tccggcacga taagaaggga ttatttacgt cgctgacgca
     8821 gactcatcaa cacagcagca aaaccaaaca gtgccgtcag cacccacagc ggccagttgc
     8881 cggtacgtgc gtatggtgtg agtccggtgg tcggcgtcac gttagtggtt aacacctcgc
     8941 gggtgaactg cgggatcatc gcctgaatct caccctgcgg gccaatcacc gccgtaatgc
     9001 cgttgttggt gctgcgcaac agtgggcgcg ccagctccag cgcacgcatt cgcgccatct
     9061 ggaagtgttg ccatggacca atagatttac caaaccacgc atcgttggag atagtcagca
     9121 ggtagtcggt atccgggcgg aagttatcgc gcacttgctc gccgagaatg atctcgtagc
     9181 aaatagccgc agtaagctca ataccatttg ccgacagcgg cggctggata tatggcccac
     9241 ggctgaacga cgacatcggc agatcaaaga acggtgctaa cggacgcaga atcgactcca
     9301 gcgggacaaa ctcgccaaac ggcaccagat ggtttttgtt atagcgatcg gctgattcgt
     9361 agctgtacgg tgcaccttta cccagcgtga tgatggtgtt gtaggtatcg tagcggttct
     9421 gcttattgag acgcgcgtcg acaatcccgg ttaccagcga gctaccttta tcacgcaact
     9481 caccgtccag tgctttgagg aacggttgct gattaatttc cagatcggtt atcgccgact
     9541 ccggccagat aatcaacgat gatttgccca tcagcggtgc cgttgcgttg tagtaaatct
     9601 ttagcgtatt aagaagctgg ccttcgtccc atttcagcga ttgcggaata tcgccctgaa
     9661 ccatcgaaac ctgaatggtt ttctccggtt gcggggtaaa ccattggatg taacgcagcg
     9721 ggaagggtag ggcaaacagc acgacggcca ccaccagcgg acgccagttg cgtttgacca
     9781 acgccagtgc cagcaggcca ctaaccatca tcagcaggaa gttaatggct tccacgccca
     9841 ttagcggtgc cagccctttt aacgggccat caatctggct atagccgaac tgtaaccacg
     9901 ggaagccagt cagcacccaa ccgcgcagaa actcggtcac ttgccagaga gcaggggcgg
     9961 caatcgccac gcgcagccag gtggttttcg gccacagacg cgacagcacg ccagcaaaca
    10021 gtccggtata cagcgacaaa tacgccgcca gcagcaccac caggaagatg tttaccgggc
    10081 caggcatccc gccaaaggtc gcgatgctga catagaccca gttaataccg ctgccaaaga
    10141 ggccaaatcc ccagcaaaag ccaatagcgg cagactggag tgggcgacgg ttaaaggtca
    10201 acgcctgaag ccccatcagc gaaataatcg ccgcaggcca gacgtcgtaa ggagagaagg
    10261 ccagcgttcc gcaggcaccg aataataacg ccagcagcag gcgaatgcgc tggcgttcaa
    10321 ttaatgaggc aaaagccatg tagttatcta tccagtttcg gttttattca tccagctttg
    10381 gctggggtga gtcatccggg attttgacat gaacctgaat aatacgccga ctatcggcca
    10441 tcgccacttt gaactggtaa ccgtcgatgt cgatagtttc gccacgcgcc ggaagatgcc
    10501 caaacgcctg catcaccaga ccaccgatag tgtcgacttc ttcatcgcta aagtgggtgc
    10561 cgaacgcttc gttgaagtct tcaatggaag ccagtgcgcg cacggtccag gtatgacgac
    10621 tcagctgacg gaagtcgata tcatcttctt cgtcatactc gtcttcaatc tcaccaacaa
    10681 tcagttccag gatgtcttca atggtcacca gaccggaaac cccaccgaat tcgtcaataa
    10741 cgatcgccat gtggtaacgc tgagagcgaa actctttcag catccggtct acgcgcttac
    10801 tttcaggaac gacaaccgcc tgacgtaaca ctttgtccat gctgaaggct tcagcatcgc
    10861 tgcgcataaa cggcagcaag tctttcgcca tcagaatccc ttcaatgtga tctttgtctt
    10921 cgctaatcac cgggaaacgt gagtgggcgg actcgatgat gacatcaagg cattcgtcca
    10981 gcttctggtt gcgtttcagg gtaatcatct gggagcgggg gatcatgatg tcgcgaacgc
    11041 gttggtctgc gatgtccatc accccttcga gcatatcgcg cgtatcttcg tcgataaggt
    11101 cgttctgccc ggaatcacgg atcagcgcca gcagttcgtc acggtttttc ggttcaccgt
    11161 ggaaaagttg gctgagtaac agggagaaaa atcccttctt gttgcttatc gtgtcactac
    11221 tgtgtgaatt gtcgtcgctc atggcgtcgt atgggttctc atgttagtta atcaaaacgc
    11281 cgtcgttaat caccaacggc ggggacgtct gccagtcaaa tgcctggcaa attattcttt
    11341 ctcggcaatg tacggatcct catagcccag agcaagcata atctctgttt cgagggcttc
    11401 catttcttct gcttcgtcat cttcaatgtg atcgtaacct aacaaatgca gactgccgtg
    11461 caccaccata tgcgcccagt gcgcctccag tggtttgcct tgctcctgag cttccttctc
    11521 aaccacctga cggcagataa ccagatcgcc cagtagcgac atctccatgc caggcggcac
    11581 ttcaaacggg aaggagagca cgttggtcgg cttatcctta ccgcgatagg tcagattcag
    11641 actgtggctt tcggcggtat cgaccacgcg aatagtcact tccgactctt cctgaaactg
    11701 cgggatcacc gcattcagcc atgtttgaaa ctggctctct tccggtaacc cggaattatc
    11761 ttcacatgcc agttgtaaat cgaggatcac ctgactcatt tttgttcctg ttcttcgcgc
    11821 ttgcgttctg ctgccagcgc cgctttacgt ttttgttctg cttcttccca ggcttcataa
    11881 gcgttaacga tacgcgccac caccgggtga cgaaccacgt cttcgctgtg gaagaagtta
    11941 aagctgatct cttcgacatc ggccagcact tcaatggcat gacgtaagcc tgatttagta
    12001 ttacgcggca ggtcgatctg tgtgacgtcg ccggtgataa ccgcttttga gttaaaaccg
    12061 atacgggtca ggaacatctt catctgttcg atggtagtgt tctggctctc atcgagaatg
    12121 ataaacgcgt cgttcagcgt acgaccacgc atataggcca gcggcgcgac ttcaataacg
    12181 ttgcgctcaa tcagtttctc gactttctca aagcccagca tttcaaacag cgcgtcgtac
    12241 agtgggcgca gatacgggtc tactttctgg cttaaatcgc caggcaggaa gcccagtttc
    12301 tcaccggctt cgaccgccgg acgagtcagc agaatacggc gaatttcctg acgctccagg
    12361 gcatcaactg ccgcagccac tgccaggtag gttttacccg tacccgccgg gccaacgccg
    12421 aaggtaatgt catggtcgag aatattggcg atgtactgcg cctggtttgg cgtgcgcggc
    12481 ttaattacgc cgcgtttagt tttgatattg accgctttgc cgtacttcgg cacgctctcc
    12541 gcgctttgct ccagtacccg cgcttcttta atcgcaaggt ggatctgttc cggttcgata
    12601 tcctgaatct gaccgcgcat cggggcagta tcgacataca ggctacgcag aatgtctgcc
    12661 gcagcggtga cgcaaatcgg acggccggtc agtttaaagt ggttatcgcg gcgattgatc
    12721 tcgatgccga gacggcgttc gagctgcttg atgttgtcat caaacgggcc gcacaggctc
    12781 aacagacgcg cattgtctgc tggctccagg gtgatttcgc gagtgtctat gttcaaaccg
    12841 ttcctcttat ctgtatgccg ccggaagctg aacattcacc ggcctataag gaaattattc
    12901 acgccacagg aaaaaggcgc aagcattgcg ataaagatgg ggataaagag agaaaaaaca
    12961 aggcccaccg gaacggcagg cctgagaatt acggctgata ataacccacg ccaaggtcgt
    13021 tttctttgcg ggtgcgggca atcaccgact ccggtgtttc tgccatgcgc agacccattt
    13081 catcttcagt tcgcaccact ttaccgcgca gagagttcgg gtagacgtcg gtaatttcta
    13141 catcgacgaa tttaccgatc atatccggtg tgccctcgaa gttgaccacg cggttatttt
    13201 cggtacgccc ggaaagctcc atgatgctct tacgcgaagt cccttctacc agaatacgct
    13261 gggtggtgcc gagcatccga cggctccatg ccatcgcttg ctgattgata cgttcttgca
    13321 gaatatacag acgctgcttc ttctcttctt ccggaacatc atcaaccata tcggcggcag
    13381 gcgttcctgg acgtgcagag aagataaagc tgtagctcat gtcgaaattg acgtcggcaa
    13441 tcagcttcat cgttttctcg aagtcttcgg tggtttcgcc agggaagcca acaatgaagt
    13501 cagaactgat ctgaatatcc ggacgcgccg cacgcagttt acggatgatc gctttgtact
    13561 ccagcgccgt atgggtacgt cccatcaggt tcagaatgcg atcggaaccg ctctgtaccg
    13621 gcagatgcag gaagctcacc agctcaggcg tgtcgcggta cacttcgatg atatcgtcgg
    13681 tgaattcgat cggatggctg gtggtaaagc gaatacgatc gatcccgtcg atcgcagcaa
    13741 ccagacgcaa cagatcggca aacgatccgg tggtgccgtc gtagttctca ccacgccagg
    13801 cattcacatt ctgaccgagc aggttgactt cacgcacgcc ctgagccgca agctgggcaa
    13861 tctcaaacag aatatcgtca gacggacggc ttacctcttc accacgggtg taaggcacca
    13921 cgcagtaggt gcaatattta ttgcagcctt ccatgatgga gacaaacgcg gtcggccctt
    13981 cggcgcgcgg ttccggcaga cggtcaaact tctcgatttc cgggaagctg atatctacaa
    14041 ccgggctgcg gtcgccgcgc acggagttga tcatctccgg cagacggtgc agcgtttgcg
    14101 gcccaaaaat aatatcgaca tagtgggcgc gctggcgaat gtgctcgcct tcttgcgatg
    14161 ccacgcagcc accgacgccg ataatcaggt ctggattctt ctcttttaac agtttccagc
    14221 gacccaactg atggaagact ttttcctgag ccttctcgcg gattgagcag gtgttcagca
    14281 gtagcacatc cgcttcttcc gccacgtcgg tcagttgata gccgtgggtg gcatccagca
    14341 gatcggccat cttcgatgaa tcgtactcgt tcatctgaca gccccaggtt ttaatatgga
    14401 gttttttggt catcgacttg ctcttgcgaa atagtagcca ggaatgcagg gcgcatagtg
    14461 taatgctttg ctgccgttgt gaccagtatg agcgttatca gcccttgggg gtaaaaatcc
    14521 tgtaaactta aagcatattg ctaacaggat gattgaccat gacaaatcaa ccaacggaaa
    14581 ttgccattgt cggcggagga atggtcggcg gcgcactggc gctggggctg gcacagcacg
    14641 gattttcggt aacggtgatc gagcacgcag aacctgcacc gtttgtcgct gacagccaac
    14701 cggacgtgcg gatctcggcg atcagcgcgg cttcggtatc attgcttaaa gggttagggg
    14761 tctgggatgc agtacaggct atgcgttgcc atccttaccg cagactggaa acctgggagt
    14821 gggaaacggc acatgtggta tttgacgcag ccgaattaaa gttgccgttg cttggctaca
    14881 tggtggaaaa cacagtcctg caacaggcgt tgtggcaggc gctggaagcg catccgaaag
    14941 taacgttacg tgtgccaacc tcgctgattg cattacatcg agataatgat cttcaggagc
    15001 tggaactgaa gggcggtgaa acgatccgcg cgaaactggt gattggtgcc gacggcgcaa
    15061 attctcaggt gcggcagatg gcgggaattg gcgttcatgc ctggcagtat gcgcagtcgt
    15121 gcatgttgat tagcgtacag tgtgagaacg atcccggcga cagcacctgg cagcaattta
    15181 ccccggacgg accgcgtgcg tttctgccgt tgtttgataa ctgggcatcg ctggtgtggt
    15241 atgactctcc ggcgcgtatt cgccagttgc agaatatgaa tatggcgcaa ctccaggcgg
    15301 aaatcgcgaa gcatttcccg tcgcgtctgg gttacgttat accgcttgcc gctggtgcgt
    15361 ttccgctgac gcgccgacat gcgttgcagt atgtgcagcc ggggcttgcg ctggtgggcg
    15421 atgccgcgca taccatccat ccgctggcgg ggcagggggt gaatcttggt tatcgtgatg
    15481 tcgatgccct gattgatgtt ctggtgaatg cccgcagcta cggtgaagcg tgggccagtt
    15541 atcctgtcct caagcgttac cagatgcggc gcatggcgga taacttcatt atgcaaagtg
    15601 gtatggatct gttttatgcc ggattcagta ataatctgcc gccgctgcgt tttgtgcgta
    15661 atctcgggtt aatggcggca gagcgtgctg gcgtgttgaa acgtcaggcg ctgaaatatg
    15721 cgttagggtt gtagccttac aacattgccg gatgcgtgcc aaccgtaggt cggataagac
    15781 gcggcagcgt cgcatccgac attgaaggat aagacgtgtc aacatcgcat tcgacattga
    15841 atgaacgcag aaaagcaaaa agctcgccga agcgagcttt tttaatgtgg ctggg
//
LOCUS       DADKXM010000089        15405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.090, whole genome shotgun sequence.
ACCESSION   DADKXM010000089 DADKXM010000000
VERSION     DADKXM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.090"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..733
                     /locus_tag="JQR05_002719"
     CDS             <1..733
                     /locus_tag="JQR05_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY5324857.1"
                     /translation="NCVEGNTTLYALPKPEVVLRWREQTTDDFRFCFKFPATISHQAA
                     LRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFLDSLPAEFNYGVEV
                     RHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIRDAQRKKPKVPVHA
                     VLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHT
                     LWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            786..1181
                     /locus_tag="JQR05_002720"
     CDS             786..1181
                     /locus_tag="JQR05_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324858.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1222..1965
                     /gene="cmoA"
                     /locus_tag="JQR05_002721"
     CDS             1222..1965
                     /gene="cmoA"
                     /locus_tag="JQR05_002721"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY5324859.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1962..2933
                     /gene="cmoB"
                     /locus_tag="JQR05_002722"
     CDS             1962..2933
                     /gene="cmoB"
                     /locus_tag="JQR05_002722"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY5324860.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3098..5527)
                     /locus_tag="JQR05_002723"
     CDS             complement(3098..5527)
                     /locus_tag="JQR05_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY5324861.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKNIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNTL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKSAVQA"
     gene            complement(5552..6652)
                     /locus_tag="JQR05_002724"
     CDS             complement(5552..6652)
                     /locus_tag="JQR05_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY5324862.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7040..7786)
                     /gene="cutC"
                     /locus_tag="JQR05_002725"
     CDS             complement(7040..7786)
                     /gene="cutC"
                     /locus_tag="JQR05_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY5324863.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYCDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGTWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7800..8366)
                     /locus_tag="JQR05_002726"
     CDS             complement(7800..8366)
                     /locus_tag="JQR05_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5324864.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8582..10315
                     /gene="argS"
                     /locus_tag="JQR05_002727"
     CDS             8582..10315
                     /gene="argS"
                     /locus_tag="JQR05_002727"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY5324865.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAAYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10492..10980
                     /locus_tag="JQR05_002728"
                     /pseudo
     CDS             10492..10980
                     /locus_tag="JQR05_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(11098..11490)
                     /locus_tag="JQR05_002729"
     CDS             complement(11098..11490)
                     /locus_tag="JQR05_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY5324866.1"
                     /translation="MRILLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(11490..12872)
                     /locus_tag="JQR05_002730"
     CDS             complement(11490..12872)
                     /locus_tag="JQR05_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY5324867.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVNFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(12869..13489)
                     /locus_tag="JQR05_002731"
     CDS             complement(12869..13489)
                     /locus_tag="JQR05_002731"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324868.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTTLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(13514..14707)
                     /gene="flhB"
                     /locus_tag="JQR05_002732"
     CDS             complement(13514..14707)
                     /gene="flhB"
                     /locus_tag="JQR05_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY5324869.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNAPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRKPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            14943..15401
                     /locus_tag="JQR05_002733"
     CDS             14943..15401
                     /locus_tag="JQR05_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY5324870.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFGHLKWSTKTGHRVRVFPVSIFRFVWG"
BASE COUNT         3767 a         4082 c         3717 g         3839 t
ORIGIN      
        1 taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg
       61 ctggcgtgaa cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc
      121 gcatcaggca gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc
      181 accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg
      241 ggagctgcct gcgctttggc attttctcga ttctctccct gccgaattta attatggtgt
      301 ggaagtccgc catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg
      361 tttacatcag cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcatcacg
      421 tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc
      481 tgtactgacg gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa
      541 ccgggaatta tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc
      601 ttatcttttt ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg
      661 ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc
      721 ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag
      781 tttgtatggt aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt
      841 ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg
      901 aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg
      961 tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca
     1021 tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct
     1081 ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc
     1141 tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc
     1201 tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc
     1261 agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag
     1321 cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc
     1381 gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg
     1441 gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg
     1501 atgattgaac gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc
     1561 attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt
     1621 accctgcaat tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga
     1681 ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt
     1741 ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg
     1801 gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc
     1861 cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt
     1921 aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact
     1981 tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga
     2041 ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg
     2101 aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa
     2161 gcgaagagcc actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga
     2221 tgccatggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt
     2281 ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc
     2341 tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc
     2401 tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac
     2461 tactgggtaa cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac
     2521 tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg
     2581 agcatctctg gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc
     2641 tggttattga tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc
     2701 gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt
     2761 ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg
     2821 aatggatggt taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag
     2881 ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg
     2941 gtattactgc cggatgcggc gtgaacgcct tatccggcct acaaaccctt gctaattcaa
     3001 tctattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc
     3061 ataacctcag cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcagacttat
     3121 caaatgccgt taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt
     3181 taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac
     3241 tgccgttttt acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca
     3301 cgcctttttt gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc
     3361 agacacgcac cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag
     3421 tgatcggttc acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac
     3481 tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac
     3541 ccttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt
     3601 tttccagcgt tttggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca
     3661 ccggatcggc gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca
     3721 tttcgatcag tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg
     3781 cacgggcccc tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac
     3841 cttcggtata gatctctttt ccgccaggtt tgagtaattc cgcaagatcg gcaaacacgt
     3901 caaaatcgtt acgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct
     3961 ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta
     4021 atacgatatc tgcgtgtttg gctgctgcgg tccagtagca ttcagaaacg acgatcatct
     4081 ccggtttctg ccaggctttg atcagacgat tagtatcctg gtgatgggta aagttaccgc
     4141 cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat
     4201 atttgccgcc aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgtcattc
     4261 cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca
     4321 cgccgccaac acgcgtcggg ttgccaccat tggagtaatg ataagagaaa ccaaaaccgc
     4381 cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct
     4441 gttcaccata ttgctggcgc tgaattcccc agcctgccat cagcattgtg cgattcgccg
     4501 ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc
     4561 agaccgcgct cttcggcgta ttatcgctct tacctgtcag atactcttca aactgcggat
     4621 acccggtagt gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat
     4681 gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat
     4741 tatcgccaaa gaattcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc
     4801 cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa
     4861 tttttaaggt attcagcgtg ttcattcccc acagcaccac aacctggctg ttttccagaa
     4921 tcagcggcca ggaggcctgc tgttcataca cctctacaga accgaccaca tgcggcatga
     4981 tcacctgtgc tgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt
     5041 tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt
     5101 aagatccggc aaaaatggcc gatggtccgt tagctttacg aatacgctca tgttgctcat
     5161 gaatcagttt taatgcctgt tcccagctca cctgtacata ggtatcttcg ccacgacctt
     5221 tcgccggttg cagtggattg tcgagatagc tttttctcac catcggatgc tgaatacgcg
     5281 ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg tttttcgcca
     5341 gcgcacctgt cgaagaaaca atcttgccgt ccttcacttc tacgctcatc gctccccaac
     5401 gtcccgcggt gaggatttta ccgcccttct cttctgccca tgcggggagc ggtgctgccg
     5461 atgtcaccac cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta
     5521 atgccataac ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt
     5581 tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc
     5641 ccattccttt ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagagg
     5701 catgacaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac
     5761 ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc
     5821 cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta
     5881 atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca
     5941 tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat
     6001 cggtaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat
     6061 gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat
     6121 ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc
     6181 cgcccttttg tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca
     6241 tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc
     6301 atactgtttc ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc
     6361 cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca
     6421 tccctgactt tggaatatgg caatcggcac attccgcacg tatccctttc tggttcgaaa
     6481 agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa
     6541 atgctgtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa
     6601 ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct
     6661 ctaaataaat tccagaacac cgaaatcatt gtataattaa atacacaacg aattaaccgc
     6721 ttgcataact cggcacaaca ctgcctgaaa taatcgataa agaatatgat ttattacaat
     6781 gtaatcatta attgctaagg aataacccag ttgctattta gaatatatat ttatcagttt
     6841 tagcaattta aatcccataa tcaatgtgaa tataaaaaat atttttagca caacgctatg
     6901 cgcactgtca cccgctcaac agatcacggt tatcgttcgt tttttatact gctcagggat
     6961 aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga
     7021 catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag
     7081 caaccgccgc cccgtctacg atataacgcg aatactcgtc cgcgtgttca tctgatgaca
     7141 tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgttccc gcggagctat
     7201 ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc
     7261 cagccataat gattggagca tcacgatggg caataagttc cataattttt gataaacctt
     7321 gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat tccgcaagat
     7381 tattgagtgt atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca
     7441 gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc ccgtcaacat
     7501 cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa
     7561 gaatggcggc aaactcaccg tcgcagtagc aaaaatcacc accgcgtggg cgaattatcg
     7621 gatgcacagg gatcgtcacc cgctggcgca cggatttcag tacccccaac gacggcgtta
     7681 agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg
     7741 ttagtgcaca ttccatgctg taacagcaaa tttccagtaa tgccattttt gctccttaat
     7801 tacgctgact gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt
     7861 gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc accttttggt
     7921 gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc
     7981 gcacgggcat tcagcgtttc tggatcaccc ggcagaacga tttcaatatg ttcccaacct
     8041 tcgtgtgggt aacgtttttc cccgggccac ggtagctcca cattggaaaa ctgccagtgc
     8101 gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcatattt
     8161 tctgacaaaa gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc ggtgacgttt
     8221 tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatatccaa ccccagacgg
     8281 cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc
     8341 tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt
     8401 taccctgttg ccctgtgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga
     8461 aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactcccg
     8521 ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc
     8581 ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc
     8641 gcctgcggat tgcgaaccgc agattcgtca gtcagcaaaa gttcagttcg gcgactatca
     8701 ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac aattagcaga
     8761 gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc
     8821 aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct
     8881 ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg ttgactactc
     8941 tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca ttattggtga
     9001 cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg
     9061 cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa
     9121 cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga aaaagcatta
     9181 cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg
     9241 tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga cgcagaacca
     9301 gatcacttac gatcgtctca acgtgacgct gacccgtgat gacgtgatgg gcgaaagcct
     9361 ctacaaccca atgctgccag gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga
     9421 aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa aacaaggaag gcgaaccgat
     9481 gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg atatcgcctg
     9541 tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg
     9601 tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcagcct atgtaccgga
     9661 atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt
     9721 caaaacccgc gcgggtggta cagtgaaact ggcagacctg ctggatgaag ccctggagcg
     9781 tgcacgtcgt ctggtggcag aaaagaaccc ggatatgcca gctgacgagc tggaaaaact
     9841 ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa accgcaccac
     9901 ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg cgccatacat
     9961 gcagtatgct tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta acgaagagca
    10021 actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct
    10081 gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg
    10141 tgcttacctg tacgatctgg ccggtctgtt ctctggcttc tacgagcact gcccgatcct
    10201 cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa
    10261 gacgctgaag ctgggtctgg atacgctggg tattgagact gtagagcgta tgtaatcgat
    10321 ttttcgtgag agtgaagcct gatcagggtt agccgatcag gcttttttat tgccatctaa
    10381 atgtattctg aaaacggaca tgccattgtt ttctcactgt tgagtaagag gccagaagcg
    10441 taatatccag gcccaggaaa acgataacgg ttcaatttaa ggaataccgc agtgtttaaa
    10501 tttcttgtat taacattagg cattatctct tgccaggctt acgcagaaga tacggttata
    10561 gtaaacgacc atgacatttc agccatcaaa gattgttggc aaaaaaattc agatgatgat
    10621 actgacatta acgtgatcaa atcatgcctg cgacaagaat acaatctcgt cgatgcgcaa
    10681 ttaaataaag cctatggtga agcttatcgt tatatagaac aagtgccacg cacaggtgca
    10741 aaaaaacttg atactgaaca acttaacttg cttaaaaaat cacagcgagc ctggctggat
    10801 tttagggaca aagaatgtga attaatcctt tcaaatgagg atgttcagga tttaagtgac
    10861 ccttattctg aatcagaata gctatcatgt atgatcatac agaccaatac gcgaactcgc
    10921 cagttgcagc tataccgtaa ctctgaagat ttttatccaa gccctttgac aagaggataa
    10981 ttcacatctt tttggcatgt tttgttgcaa gctattcctg ataaatgatt gcaacaaaac
    11041 atccagcctt ttgactgagt tattaaacat actcgcgagc gcgtaatttt ttgtctttca
    11101 gcgataattc acaatcactt cattccgttg tacctttagc ggtggaatta accgcccacc
    11161 gcctggcact tcccagataa atcgcaacgg ttctgctgcc gctataccgg aaaaggccac
    11221 ggtggtaccg ctctgcccct ctaattcgac acaacgagat tgcgaacaca agcgaacccg
    11281 cagtcctgca ggtgtcgggc cgataagctg ataacgccac gccaccagcg tcatcaatcc
    11341 tgaagcaggt tgtcgcgcag aaagaggtgc agacgacatc gactcaccgc gatgatttag
    11401 cgtaattccg acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
    11461 aaacaataat atcgctaata agattctcat catttaccgc caattgtcgc cgtcatgcgg
    11521 atatgtcggt tatcagacag ttccagattc gacaggacca ctaactgcgg caagctgcgg
    11581 cgcaggaagc gagacaataa tggtcgcagc gcgtggttca ccaacaatac tggcggcgca
    11641 cccagcatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
    11701 cctggctcca gtcctccccc gccctgcagc gcctgtagta acaaacgttc cagcggtgta
    11761 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
    11821 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
    11881 tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg gtactttttc atcgaggaga
    11941 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc caggaacgag atcttccgtc
    12001 agctttggca tctcctgggc gacgcgatcc aacagctgtt gcgcctcctg acgaccaaac
    12061 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
    12121 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
    12181 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
    12241 ccagggttaa tcgccagcca gcgccccgga taagcatcac cactgccaat ctccacgcct
    12301 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgcaccact
    12361 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
    12421 aactcaccat cctgctggaa attgaccatc gggatcagtc gataacccac ttccattccc
    12481 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggtatt attctctgcc
    12541 atttttaccg gtttgggttc ggcaggcgct ttttgttcgc gtccgcgtat ccaccaggcc
    12601 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
    12661 ccgagtaaac cgagcacggc ggcgcttaac aacataacgc ttgggttact gaaaagctga
    12721 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacgat gccccccgcg
    12781 gcggtagaaa tcaccagcgc cggaatttgt gccaccagac cgtcgccaat ggtcaatagc
    12841 gtataacttt ccgccgcgtg tcccatgctc atgccagttg cagcacgccg accagcaacc
    12901 cgccgacaac gttaatgacc atgatgagga tcccggcaat ggcatcgccg cgaacaaact
    12961 tacttgcccc gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt
    13021 ttttcgcctc atcttcacca atcaatccag cgttaaggtc ggcgtcaatc gccatctgct
    13081 tacccggcat accatcgaga acaaagcgcg cccccacttc tgcgatacgc ccggcacctt
    13141 tggtaatgac cataaagttg atgatcacga gaatgacaaa caccacgata ccgatagcga
    13201 aattgccacc aacgaggaag tgaccgaacg cttcgaccac cttcccagcc gccgccgcgc
    13261 cggtatgccc ttccatcaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca
    13321 acgtagtaaa caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca
    13381 tcgccaccag caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta
    13441 tgaatgcggg cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg
    13501 caaggatctg ccattatgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat
    13561 tactcatggg tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta
    13621 ggtgtacagg gcgctgtcca ccagccagac gccagcgttt cagttgccag acccaggcca
    13681 gcacttccgc caccgcggcg tacagttgac ccgggatttg ttgaccaatc tccgcatgtc
    13741 gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg ggcgttattt tcagcgccaa
    13801 tttcacgaat acgcagcgcg accagccctg cacctttagc gaccactttc ggtgcgctca
    13861 ttttgttttc gtcatactgc aacgctaccg aatagtgggt cgggttattg acaatgacat
    13921 ccgctttcgg cacatcggcc atcatccgac gccgtgcggc agctcgctgc atctgacaga
    13981 tccgtccttt aacatggggg tcgccttcgc tttgtttgaa ctcatcacga atatcctgcc
    14041 gcgacattcg cagctttttc aggtggctga agatttggaa aaagacgtca aatcccacca
    14101 ttggaatgac accaagcacc accagcagtg cgcatagccc taccaaatcc atcgcattac
    14161 ccatggcggt aatcggtgac tcggccatta agcgcatcat ctgcggccag tgatgccaga
    14221 gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa aattgcctta agcaactccg
    14281 cgctagtctg tgccgagaat atccgtttaa tgcccggtag cgggttgagt ttggaaaact
    14341 tcggctgcaa ggatttgccg ctaaatacca gcccccccag cataaccgga gaaatgagcg
    14401 ccaccagcac cacgccgcta atcagcggta gcagcgccag catggcttct ctgatcagca
    14461 gaataatttg cccgaggatc agattcgggt ctttgataat actgtgatca aaatgcagcc
    14521 cagcggagag catgcccgac aatcgacggg ccagcgacac accgccaaac cagataacac
    14581 taacgcccac cagcaaaatc agcagtgagg tcagttcacg ggaacgcggg atttgcccct
    14641 cttcccgcgc tttttctagt cggtgaggtg tgggggcttc tgttttgtcg tcgctctcgt
    14701 cagacacgtc gccaatcctg gaaagagtat taagcgtaaa tgatgccaga gcgcaaagcg
    14761 ttcaatggtt taagtaaggg gcaaaacagg cgggatttag ggcgtttatg caaaaatagg
    14821 ttcagttgca gaacgttttg cgaaccattt tccagttctg atatttagcc atttagccta
    14881 tagaatcgcc ctccgcctct ctgttagttt gctaataatt aaattattac aaaggtgatc
    14941 aaatggaatg gcaacaattg cttaactgca accgtcggaa agataatcta aaaaaaacca
    15001 acatagcatt tttacatccc cttgctagcg gaagacaaga gatcgagcgg gattacgacc
    15061 gattactttt tgctgcacca actcgtcggc ttgcggataa aacacaagtt ttcccgttag
    15121 atcaaaatga cagcatacgg acgaggctga ctcattctca cgaagtagca aactttgctc
    15181 gcgggatagg aatgaggctc gctttcgaga tgaaggagca catctttggc gaaataccca
    15241 cggggattgt cgttgaacgc gacgttcctg ctctgttagc agcgatcgga ctggcacacg
    15301 atctcggtaa tccaccattt gggcatctga agtggtcaac aaaaactggc caccgagtta
    15361 gagtttttcc agtatcgatt ttccgattcg tttgggggta accca
//
LOCUS       DADKXM010000090        15372 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.091, whole genome shotgun sequence.
ACCESSION   DADKXM010000090 DADKXM010000000
VERSION     DADKXM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.091"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQR05_002734"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQR05_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY5324871.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JQR05_002735"
     tRNA            complement(621..695)
                     /locus_tag="JQR05_002735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="JQR05_002736"
     CDS             complement(771..1703)
                     /locus_tag="JQR05_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY5324872.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTDIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTPHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="JQR05_002737"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="JQR05_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY5324873.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2934..3993)
                     /locus_tag="JQR05_002738"
                     /pseudo
     CDS             complement(2934..3993)
                     /locus_tag="JQR05_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4359..5699)
                     /gene="fadL"
                     /locus_tag="JQR05_002739"
     CDS             complement(4359..5699)
                     /gene="fadL"
                     /locus_tag="JQR05_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY5324874.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6071..6355
                     /locus_tag="JQR05_002740"
     CDS             6071..6355
                     /locus_tag="JQR05_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY5324875.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6535..7845
                     /gene="fadI"
                     /locus_tag="JQR05_002741"
     CDS             6535..7845
                     /gene="fadI"
                     /locus_tag="JQR05_002741"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY5324876.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVLLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7845..9989
                     /gene="fadJ"
                     /locus_tag="JQR05_002742"
     CDS             7845..9989
                     /gene="fadJ"
                     /locus_tag="JQR05_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY5324877.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAALNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPFIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10192..10677
                     /gene="sixA"
                     /locus_tag="JQR05_002743"
     CDS             10192..10677
                     /gene="sixA"
                     /locus_tag="JQR05_002743"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY5324878.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            11352..11915
                     /locus_tag="JQR05_002744"
     CDS             11352..11915
                     /locus_tag="JQR05_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5324879.1"
                     /translation="MSKFVKTAIAVAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            11997..14638
                     /gene="yfcU"
                     /locus_tag="JQR05_002745"
                     /pseudo
     CDS             11997..14638
                     /gene="yfcU"
                     /locus_tag="JQR05_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            14658..>15372
                     /locus_tag="JQR05_002746"
     CDS             14658..>15372
                     /locus_tag="JQR05_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY5324880.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGN"
BASE COUNT         3908 a         3551 c         3822 g         4091 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaattgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacaaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatggggg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatatc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac
     2881 tgactatttc cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata
     2941 tcatcgaaat taacacctag tgcagatagt tgattaattt cattttgata tttcgcctgg
     3001 cgattttgaa gtcttttttc aactttatct tcgagatcct taaagtcttt gtatttattt
     3061 tcaggatggt caacgtcaac tttgttagcg aataaagtag aaattatttc ttttacatta
     3121 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     3181 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     3241 tcggaaccag ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa
     3301 gcactcctgg cgttttcact acaatattgg gtgagactat caccattaat acgaattcct
     3361 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     3421 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     3481 aagtccgttg gttcgctttt cccgctctca tcaaattccg ttaccgtatg tctgagtttg
     3541 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     3601 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     3661 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     3721 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa
     3781 ttattaatat taaatctata aggatcgtcg tttactcgta tggcgttttt tataagctca
     3841 agtagatgtt taatacaggt gcttattcct tccttaccat taaaaatggt ttttgaactc
     3901 ttgtactaat ccattaattt catcattgtt atttttattg atggattctg ggtaagctgc
     3961 gctggtattg ttgattgaaa tagatggtag catgatttta ttccatgttg attgatttgt
     4021 tacaaatata tattacctaa tgaaatagac tatcaaaaaa cgctcattta tttagaacct
     4081 atctttaatt tgagtgtata agcgcacaat agccaggggg gttgagtatc ctggaaatgc
     4141 agaaaagtaa actctttgag aaggacgagc agaacgcttg aaaatatgca tttcccttca
     4201 ggtttgtctg tggataccac ttactgattt gaaagggcaa gctgccatca ggctgtttgc
     4261 aaaactcagg aggcaagcaa tgaggaaact actttcggtg aagtggggga gttaaagtca
     4321 cctgcatagc aggtgacttt atccaggcga acgcgttatc agaacgcgta gttaaagtta
     4381 gtaccgaaca gccaggcttt accttcagac tcgaactggt atgggccttc gttaattttc
     4441 acgctctgac cgtgcatata agaaacacca acgtcgactg aagcatcttt attaaatgcg
     4501 taagtcgtac ctgcactcag ccagaaacgg tcctggtccg gaatggagat agaacgattc
     4561 tgagccggaa ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca gttatcatcg
     4621 tagtaataag tggtacccaa cgcgatgcgg taagcatctt taaagccttc atgtttctgg
     4681 aacagcgtgt cgccactggt tgaggtcgct ttcagctgct ggaactgact ccagctggtg
     4741 taagccaggc tatagtgaat cgcccactgt ggatcaacac ggttataacc tgacacttcc
     4801 cacatttcag gcaggttcag cgtcagataa cccgattgcg ttgcgccacc tgtcgcggta
     4861 ggaattggca aaccgtagtt attaaacgca cgattaagat cgctgctgta gttacctttg
     4921 aagtcaattt tcacttcaga acggtaggtc agtgcatagc ggttattttt atccagttca
     4981 tacaggattc cggcgttcca gccaaagccc cactggttac cgttcagatg agcgattttg
     5041 gtattactgt cgataccgtt ggcggtagct gccaatgctt gcccttgagg agtttgcccg
     5101 gcaggagatt gcataatctg accagcaacc aactgcccca gatcgcctgc gaaacgttca
     5161 attttcgcgc gagcgtagat ggcgttgaaa ccaagaccaa agctccatgc attatttaag
     5221 cgatacgcac cgcttaagtt caggttcatg gtttcaaggt cagttgtacc cccgacagag
     5281 ccgcctgcat aagtatcatt aaactctgtt gccagaccat agttagaggt aatagaagcg
     5341 ccccaaccaa attggtcgtt aatcggtgca acaaagtgca tgttcggaac ccatgccgta
     5401 ggcgcgatgt tatcggcttt caggctacga ccagacggag acgttccgct gatattcaca
     5461 tccgggtcaa tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat ggtaatcaat
     5521 gcggggttac ggctaacgtt acctgcatca tcggcaattg cgccttcccc tgaataagcc
     5581 cggcccaggc cagaggaaga aaattcgttt aactgaaagc ctgccgacca ggcctgggtg
     5641 gagataagtg ccactgcgac tgcgagagca gactttgtaa acagggtttt ctggctcatg
     5701 accataacct caatgattta tttttataca aactatgtta cgtgctgtaa caggagcgcg
     5761 aagtgtaggg tctgaggtac atcttagaaa tcagaccagt ggcgagagta taggtcggac
     5821 cagctagaat gttgcaagta tgtttccatc ttttttcaaa tatgatctaa gaaacgcgat
     5881 ccagctggca aaatttggag gttgcgcact cgcggattcg ggtgaatttt gttttagatc
     5941 atttttaagt gtgatttcgg tcacttatcc gattttgcag aattaacaac tggagcagca
     6001 ctttccggaa gcgtaaaata tccacaacgt ttatctggca ccttgggtgc acatacagag
     6061 gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg gtttgctgct gtatggatgt
     6121 tggcaccatt atggacaact ccgactgcac cgcgtcttac agccgcgtgt ttgcaaaccg
     6181 cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa gcccgtagcg tggaatccga
     6241 accgtgcaaa atctccccga ctttcactga agagtctgac ggtgtgcgcc tggatatcga
     6301 tttcactttt gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc gttaattcct
     6361 ctcgggtacg tacgcccctg tcagttgctg gcaggggcgt tttcttttct ttcttctgtc
     6421 ttcactgtgt catagctccc actttccttt ttctccgctt ggctaaatgt aaaaaaatgg
     6481 ttaagactat gatcaggtca gaccacttta tttatttttt tacaggggag tgttatgggt
     6541 caggttttac cactggttac ccgccagggc gatcgtatcg ccattgttag cggtttacgt
     6601 acgccttttg cccgccaggc gacggctttt catggcattc ccgcggttga tttagggaag
     6661 atggtggtag gcgaactgct ggcacgcagc gagatccccg ctgaagtgat tgaacagctg
     6721 gtctttggtc aggtcgtaca aatgcctgaa gcccccaaca ttgcgcgtga aattgttctc
     6781 ggtaccggaa tgaatgtgca taccgatgct tacagcgtca gccgcgcttg cgctaccagt
     6841 ttccaggcgg ttgcaaacgt cgcagaaagc ctgatggcgg gaactatccg agcggggatt
     6901 gccggtgggg cagattcctc ttctgtattg ccaattggcg tcagtaaaaa actggcgcac
     6961 gtgctggttg atgtcaacaa agctcgaacc atgagccagc gcctgaaact cttctctcgc
     7021 ctgcgtttgc gcgacttaat gcccgtaccg cctgcggtag cagaatattc tactggcttg
     7081 cggatgggcg acaccgccga acaaatggcg aaaacctacg gcatcacccg agaacagcaa
     7141 gatgcattag cgcaccgttc gcatcagcgt gccgctcagg catggtcaga cggaaaactc
     7201 aaagaagagg tgatgactgc ctttatccct ccttataaac aaccgcttgt cgaagacaac
     7261 aatattcgcg gtaattcctc gcttgccgat tacgcaaagc tgcgcccggc gtttgatcgc
     7321 aaacacggaa cggtaacggc ggcaaacagt acgccgctga ccgatggcgc ggcagcggtg
     7381 atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg tgctgctggg gtatctgcgc
     7441 agctacgcat ttactgcgat tgatgtctgg caggacatgt tgctcggtcc agcctggtca
     7501 acaccgctgg cgctggaacg tgccggtttg acgatgagcg aactgacatt gatcgatatg
     7561 cacgaagcct ttgcagctca gacactggcg aatattcagt tgctgggtag tgaacgtttt
     7621 gctcgtgaag tactggggcg tgcacatgcc actggcgaag tggacgatag caaatttaac
     7681 gtgcttggcg gttcgattgc ttacggacat cccttcgcgg cgaccggcgc gcggatgatt
     7741 acccagacat tgcatgaact tcgccgtcgc ggcggtggat ttggtttagt taccgcctgt
     7801 gctgccggtg ggcttggcgc ggcaatggtt ctggaggcgg aataatggaa atggcatcag
     7861 cgtttactct taatgttcgt ctggacaaca ttgctatcat caccatcgac gtaccggatg
     7921 agaaaatgaa taccctgaag gcggaatttg cctcgcaggt gcgcgccatt attaagcaac
     7981 tccgtgaaaa caaagagttg cgcggcgtgg tgtttatctc cgcgaaaccg gacaatttta
     8041 tcgccggagc agacatcaac atgattggca actgcaaaac ggcgcaagaa gcggaagcac
     8101 tggcgcggca gggccaacag ttgatggcgg agattcatgc tttgcccatt ccggttatcg
     8161 cggctattca tggcgcttgc ctgggcggtg ggctggagct ggcgctggcg tgccacggtc
     8221 gcgtttgtac tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa cttggattgt
     8281 tacccggttc aggcggcacc cagcgtttac cgcgtctgat aggcgtcagt acagcattag
     8341 agatgattct caccggaaaa caacttcggg cgaaacaggc attaaagctg gggctggtgg
     8401 atgacgttgt tccgcactcc attttgctgg aagccgctgt tgagctggcg aagaaggatc
     8461 gcccatcttc ccgccctcta cctgtacgcg agcgtattct ggcggggccg ttaggtcgtg
     8521 cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa aactcaaggc aattatccgg
     8581 cgacagaacg catcctggag gttgttgaaa cgggattagc gcagggcacc agcagcggct
     8641 atgacgctga agctcgcgcc tttggcgaac tggcgatgac gccgcaatcg caagctttgc
     8701 gtagtatctt ttttgccagt acggacgtga agaaagatcc cggtagtgat gcaccgcctg
     8761 cggcattaaa cagcgtggga attttaggtg gtggcttgat gggcggcggt attgcttatg
     8821 tcactgcttg taaagcgggg cttccggtca gaattaaaga tatcaacccg cagggcataa
     8881 atcatgcgct gaagtacagt tgggatcagc tggagggcaa agttcgccgt cgtcatctca
     8941 aagccagcga acgtgacaaa cagctggcat taatctccgg aacgacggac tatcgcggct
     9001 ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa tctcgaatta aaacaacaga
     9061 tggtggcgga agtggagcaa aattgcgccg ctcataccat ttttgcttcg aacacgtcat
     9121 ctttaccgat tggtgatatc gccgctcacg ccgcgcgacc tgagcaagtt attggcctgc
     9181 atttcttcag tccagtggaa aaaatgccgc tggtagagat tattcctcat gcgggtacat
     9241 cggcgcaaac catcgccact acggtaaaac tggcgaaaaa acagggcaaa acgcccattg
     9301 tcgtgcgtga caaagccggg ttttacgtca atcgcatctt agcgccttac attaatgaag
     9361 ctatccgcat gctgaccgaa ggtgaacggg tagagcacat tgatgccgcg ctagtgaaat
     9421 ttggttttcc ggtaggccca atccaacttt tggatgaggt aggaatcgac accgggacta
     9481 aaattattcc tgtactggaa gccgcttatg gagaacgttt tagcgcgcct gcaaatgttg
     9541 tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt ttctatcttt
     9601 atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc tgccatttac ccgttcattg
     9661 gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt gtgatgttga
     9721 tgctgaatga agcagtacgt tgtgttgatg agcaggttat ccgtagcgtg cgtgacgggg
     9781 atattggtgc ggtatttggc attggttttc cgccatttct cggtggaccg ttccgctata
     9841 tcgattctct cggcgcgggc gaagtggttg caataatgca acgactggcc acgcagtatg
     9901 gttcccgttt taccccttgc gagcgtttgg tcgagatggg cgcgcgtggg gaaagttttt
     9961 ggaaaacaac tgcaactgac ctgcaataag aaggtcaaag ctatatgaat ccgcgctgaa
    10021 tggcggagtg ttggtcaaaa tgtaaacgca tattgactat acttacgcca ttgaggtaaa
    10081 aaacagcgtt tcattcggtg aatggataag gcacaatgcc ggccaccgtt ttctttctct
    10141 ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa agcggtgcaa tatgcaagtt
    10201 tttatcatgc gtcacggcga cgcagccctc gatgccgcca gtgattcggt tcgtcctctg
    10261 accactaatg gttgtgacga atctcgcctg atggcgaact ggctgaaagg tcaaaaagtg
    10321 gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg agcaaacact ggaagaagta
    10381 ggggattgtc tgaacctgcc ttccagtgca gaagttctgc cggagttaac gccctgcggc
    10441 gatgttggtc tggtcagtgc ctatttgcag gcgttgacca atgaaggtgt tgcttcggta
    10501 ctggtgatct ctcacttacc gttagtcggg tatctggttg ccgagttatg tccgggcgaa
    10561 acgccgccta tgtttaccac ctcggccatt gccagcgtaa cgcttgatga aagcggaaac
    10621 ggtacattca actggcagat gagcccgtgt aatctgaaga tggcaaaagc tatctgatgt
    10681 gttccaaatt atgcccaatg aggtagattc attgggcata attgctcatt aggcagcgga
    10741 tatctgtcaa cctcactact attgaagact ctccttatct ataaataaaa cactttctac
    10801 attcattcgg aaattattct tctgcgcgta tcttgatttt gcccaaagtt acatgttgaa
    10861 atgaataatg tctatttgac actcattgtc atttattaat acagataata gagttgaata
    10921 atcaggcagg atgtaattga aacttgatta aattctggct atagtgttta tatattatat
    10981 tgtcaaaagt ggataaatgc caatcgcatt atatgttcat tgtttgtttt ttaagctgtt
    11041 gatattcatt attgatatac tttctatttt ttctaaatac ttattccgta ttttttaagg
    11101 atgtccttaa gataaccttt cttctgtcaa tatattaatt aaatctacat ttactcttta
    11161 gccaaattaa attggctcag gggtgtggaa acatcactat gcggccccac accaacgaat
    11221 acaattttct acgacctgtt ctttttgggt cgcatgccgt attcaatggc tgggtataaa
    11281 cattggctat tgactgaatc aatgtattgc aatgtttata tttttaatta tttatagttt
    11341 aaggacaaat aatgagtaag tttgttaaaa cagctattgc tgtggcaatg gtgatgggcg
    11401 tgttcacctc tacggcaaca atcgctgcgg gtaacaacgg tactgcacgt ttttacggca
    11461 ccattgaaga ctcagtgtgt tctatcgttc cggacgatca caaactggaa gttgatatgg
    11521 gtgatatcgg tgccgaaaaa ctgaaaaata acggcaccac cacgccgaaa agtttccaga
    11581 ttcgtctgca agattgcgta tttgatactc aggaaacaat gaccactacc tttactggta
    11641 ccgtttcttc tgcaaatagc ggcaattatt acaccatttt caataccgat accggtgcgg
    11701 catttaacaa tgttagcttg gcgatcggtg actctctggg tacctcttac aaaagcggca
    11761 tgggtattga ccagaaaata gtgaaagaca cttctaccaa caaaggcaaa gcgaagcaga
    11821 cactgaactt taaagcctgg ctggtcggcg cagctgatgc gccagatctg ggtaattttg
    11881 aagcaaatac caccttccag attacttacc tgtaaggcat actcccatag cgacactgct
    11941 atgggggatt taaatggatt ttgaccgaac aagaataaaa acggatagaa acgtgtatgc
    12001 ctgaccattc tctttttcga ttacggattc ttccgtggtg cattgcgctg gcaatgtcag
    12061 ggagttatag cagtgtctgg gctgaagacg acattcagtt tgattcccgt tttctggaat
    12121 taaaaggcga cacaaaaatt gatctgaagc gtttttccag ccagggatat gttgagcccg
    12181 gaaaatacaa tttacaggtt caactaaata aacagccatt ggcggaagag tacgatattt
    12241 actggtatgc tggtgaagat gacgcgagca aaagctatgc ttgtctgaca ccggaactgg
    12301 tagcgcagtt tggtttaaaa gaagacgtgg cgaaaaatct gcaatggagc cacgatgcta
    12361 aatgcctgaa atccggtcaa ctggaaggca tggaaattaa ggctgattta agccagtccg
    12421 cattagtcat ttcactgcca caggcttacc tcgaatatac ttatcccgac tgggatccgc
    12481 cttcacgttg ggatgacggc atctccggga tcgtcgcgga ctacagcatc aacgcacaaa
    12541 cccggcacga agaaaatggc ggtgatgata gtaacgagat cagcggcaac gggacggtcg
    12601 gggttaacct ggggccgtgg cgtatgcgtg ctgactggca gactaactat caacatactc
    12661 gcagtaatga tgacgatgaa ttcagcggcg atgaaactca aaaaaaatgg gagtggagtc
    12721 gctactatgc caggcgggcg ttaccatcat taaaagccaa actggcgctg ggcgaggatt
    12781 acctcagatc cgatattttt gatggtttta actatgttgg tggcagtgtc agtactgacg
    12841 atcaaatgtt gcctcccaat ctgcgcggct acgcgccaga catttccggc gtggcacaca
    12901 ccacagcaaa agtgaccgtc agccagatgg ggcgtgtgat ttacgaaacg taggttccgg
    12961 ctggaccgtt tcgtattcag gatcttggtg attctgtctc cggtacgttg catattcgca
    13021 ttgaagaaca gaacggccag gtgcaggaat atgacatcag caccgcctcg atgccatacc
    13081 tcactcgtcc aggtcaggtt cgctataaga tcatgatggg ccgtccgcaa gagtggggac
    13141 accatgtcga gggtgaattt ttttctggtg ctgaagcttc ctgggggatc gctaacggct
    13201 ggtcgttata tggcggcgca ctgggagatg aaaactatca gtctgcggcg cttggcgtcg
    13261 gtcgcgattt gtctacattc ggcgcggtcg cgtttgatgt tactcactcg cacaccaaac
    13321 tggataaaga caccgcttat ggcaaaggtt cgctggacgg taactccttc cgtgtgagtt
    13381 attccaaaga ctttgaccag ctcaacagcc gcgttacttt tgctggatat cgcttctcgg
    13441 aagagaactt tatgaccatg agcgagtatc tggatgccag tgacagcgga atggtacgca
    13501 cgggcaacga caaagagatg tacaccgcca cttataacca gaacttccgc gatgcgggtg
    13561 tttcggttta tctcaactat acccgccata cctactggga tcgcgaggag cagacaaact
    13621 acaacatcat gctctcgcac tatttcaata tgggcagtat tcgtaatgtc agcatctcga
    13681 tgactggcta ccgttacgag tatgacaacc aggccgacaa aggcatgtac atttcgctca
    13741 gtatgccgtg gggcgacaac agtaccgtta gctataacgg tactatggca gtgggacgga
    13801 cagcagtcag gtcggttatt tcagccgtgt cgatgacgcg actcactatc agttgaacgt
    13861 cggcaccagt gacaaacaca ccagcgttga cggctattac agccatgatg gttcgctggc
    13921 gcaggttgac ctcagtgcga actaccatga agggcaatac acctctgcgg gcttgtcgtt
    13981 acagggcggc gcaacgctta ctgcccacgg tggcgcactc caccgtaccc agaatatggg
    14041 cgggacacgc ttgttgattg atgccgatgg cgttgccgat gttccggtag aaggtaacgg
    14101 tgctgctgtt tataccaata tgtttggtaa agccgtcgtt tctgacgtca ataactatta
    14161 ccgcaatcag gcgtatatcg acctcaacaa actgccggaa aacgccgaag caacccagtc
    14221 ggtggtacaa gccacgctaa ctgaaggggc gattggctac cgcaaatttg ccgtcatcag
    14281 tggtcaaaaa gcgatggcgg tgctgcgttt acaagacggc agccatcctc cgtttggcgc
    14341 agaagtaaaa aatgataacg agcagacagt gggccttgtc gatgatgacg gcaatgttta
    14401 tctggctggg gtgaaacctg gcgaacacat gagtgtgttc tggagtggtg ttgcgcattg
    14461 cgatatcaac ctgccggacc cgctgcctgc cgatctgttt aacggcttgt tactgccatg
    14521 ccagcataaa ggcaacgtcg cgccggttgt tcctgatgac ataaagcctg tcattcagga
    14581 gcagacgcaa caggtgacac ccaccaatcc ccctgtttct gtttcagcta accaataacg
    14641 tgattaagga atgatccatg tctgatttac tttgctctgc caaattgggt gctacgacgc
    14701 tagcgttact gcttagcgct gccagtttgt ctgcccaggc ttctgtaacg ccagatcgta
    14761 cccgtttgat atttaatgaa agcgacaaat caatcagcgt gacgttgcgc aataacgatc
    14821 cgaaactgcc ttatctggcc caaagctgga tcgaagatga aaagggtaac aagatcagtt
    14881 caccgttaac tgttctgcca ccggtgcaac gcatcgattc aatgatgaac gggcaggtaa
    14941 aagtgcaggg aatgccggat atcaacaaat tgcctgccga tcgtgaaagc ctgttctact
    15001 tcaacgtgcg tgagatcccg ccaaaatcaa acaaagccaa cacgctgcaa atagcactac
    15061 aaacgcgtat taagttgttc tggcgaccga aggcgctgga gaacgtcagc atgaaaaacc
    15121 cctggcaata taaggttact ttaacccgca acggtcagga atttaccgtc aataacccga
    15181 cgtcgtatta cgtcattttc agtaatgcca gcacacagaa aaatggcaat ccggcagccg
    15241 gtttctcgcc aatagtgatg tcgccgaaaa cctctgcacc gctgaatgtg aagatgggta
    15301 gcgtgccggt actgacctat gtcaacgact atggcgctcg gatgccgctc tttttcactt
    15361 gcaacggtaa ca
//
LOCUS       DADKXM010000091        15369 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.092, whole genome shotgun sequence.
ACCESSION   DADKXM010000091 DADKXM010000000
VERSION     DADKXM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.092"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            116..1089
                     /locus_tag="JQR05_002747"
                     /pseudo
     CDS             116..1089
                     /locus_tag="JQR05_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1086..2222)
                     /locus_tag="JQR05_002748"
     CDS             complement(1086..2222)
                     /locus_tag="JQR05_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5324881.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(2228..2551)
                     /locus_tag="JQR05_002749"
                     /pseudo
     CDS             complement(2228..2551)
                     /locus_tag="JQR05_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
     gene            2734..2877
                     /locus_tag="JQR05_002750"
     CDS             2734..2877
                     /locus_tag="JQR05_002750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324882.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(3096..4634)
                     /locus_tag="JQR05_002751"
     CDS             complement(3096..4634)
                     /locus_tag="JQR05_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY5324883.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4799..5950)
                     /gene="yiaY"
                     /locus_tag="JQR05_002752"
     CDS             complement(4799..5950)
                     /gene="yiaY"
                     /locus_tag="JQR05_002752"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY5324884.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(6140..7984)
                     /gene="selB"
                     /locus_tag="JQR05_002753"
     CDS             complement(6140..7984)
                     /gene="selB"
                     /locus_tag="JQR05_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY5324885.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLGIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7981..9372)
                     /gene="selA"
                     /locus_tag="JQR05_002754"
     CDS             complement(7981..9372)
                     /gene="selA"
                     /locus_tag="JQR05_002754"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY5324886.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(9470..10078)
                     /locus_tag="JQR05_002755"
     CDS             complement(9470..10078)
                     /locus_tag="JQR05_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY5324887.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVLVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFACTKPPKA"
     gene            complement(10149..11234)
                     /locus_tag="JQR05_002756"
     CDS             complement(10149..11234)
                     /locus_tag="JQR05_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5324888.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHLYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(11237..11599)
                     /locus_tag="JQR05_002757"
     CDS             complement(11237..11599)
                     /locus_tag="JQR05_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY5324889.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            12136..12819
                     /locus_tag="JQR05_002758"
                     /pseudo
     CDS             12136..12819
                     /locus_tag="JQR05_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            12821..13699
                     /locus_tag="JQR05_002759"
                     /pseudo
     CDS             12821..13699
                     /locus_tag="JQR05_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            13699..14286
                     /gene="mtlR"
                     /locus_tag="JQR05_002760"
     CDS             13699..14286
                     /gene="mtlR"
                     /locus_tag="JQR05_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY5324890.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(14298..14507)
                     /locus_tag="JQR05_002761"
     CDS             complement(14298..14507)
                     /locus_tag="JQR05_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324891.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            14791..15153
                     /locus_tag="JQR05_002762"
     CDS             14791..15153
                     /locus_tag="JQR05_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY5324892.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LRREMGLEEMTGFSKTTF"
BASE COUNT         3847 a         4076 c         3743 g         3703 t
ORIGIN      
        1 ttccatttta aaataagata aaaaagagtc ggcataataa tgcttacaat ttctccggtg
       61 caagattcat atttatatag taatatataa aattatatat aattgggctg ttgcgatgac
      121 aaaattacaa cttaagtacc gggagttaaa aattatctcg gtaatcgctg ccagtgaaaa
      181 tatcagccat gccggactgt acttggcatc gcacaggcca acgtcagcaa atatcttgct
      241 gattttgaat caaaagtggg tttaaaagtc tttgaccgaa ccacccggca gctgatgctc
      301 acaccttttg gcaccgcgct gttaccgtac atcaacgaca tgctggacag aaacgagcaa
      361 cttaataatt ttattgcaga ttataagcat gagaaacgtg gtcgggtcac catctacgcc
      421 ccaaccggca taattaccta tttatccaaa catgtaatcg ataaaattaa agatatcggt
      481 gatatcacct tatcgctaaa aacctgcaat cttgagcgta atgcctttta tgaaggcgtc
      541 gaatttcctg atgattgtga tgttttaatc agctacgctc caccaaaaga tgaatcgctg
      601 gttgccagtt ttattacaca atatgccgta accgcttatg ccagccagcg ctatcttgag
      661 aaacatccta ttagccgtcc tgacgaactc gaacatcact cctgtatttt gatcgactcg
      721 atgatgatcg acgacgcgaa tatctggcgc ttcaacgtcg ccgggagtaa agaggtgcgg
      781 gattatcgcg tgaagggaaa ttacgtttgc gataacacgc aatcggcgct ggagctggca
      841 cgaaatcacc tggggattgt gtttgcgcca gacaaaagtg tgcagagcga tctgcaagac
      901 ggcacgctgg tgccctgctt tcagcacccg tatgaatggt ggctggacct ggtggctatc
      961 ttccgtaaac gggaatacct gccctggcgg gtccagtatg tgctggatga aatgctgcgt
     1021 gaaattcgcc accagcttgc tcagtcgcag caactgcggc ctgaacaagc ggcggaaagc
     1081 gaagattaat gatcgagata aaggtaatgc acccagctgg tcatgcgtaa tagcactttg
     1141 cgcatcaccg agacatggct gaaatgatca gagtagaccg ccacctgggc gtaaataccg
     1201 tctggtaaag cgctcaaatc agtgtgctca tccagttcaa tggtcgcgat aacgccatct
     1261 gaacccggcg ctgtgtttaa cgtctgtaag gtgccggtcg actgataagc tccgccggga
     1321 acagctggac taatggctgc cagcttgccg ctgaatacct tacctggcag agcattaaac
     1381 accacttccg catcgtcgcc aggggccagt cgcagcaagg agttctgacg gaactgcgcc
     1441 acaatttgtc gtttctgatc gggtataaac accatcaccg gacgtagcgg cagcgacgcg
     1501 gcataggtac ccggacgaat cagcacctgg gtcacataac catcgctcgg cgcacgcact
     1561 atcgtctgct caaggttata ttttgcttcc gcgagctgcg ctttcaggct ggcgatttga
     1621 gaatgttcac ccaacaccag gctatccagc tggctctgga tctgtttttg ttccgccgcc
     1681 gatgacttta cggaggcttc ctgcgccaga taattttgcc gcgccacatc aatatcgcgt
     1741 tctgaaaacg ggtttacttt cgcctggctg ccgcgtgcgt aacgctgata ctctttagcg
     1801 aatttatccc gcgtggcctt tgcctgctga gtattcgccg ccatctcatc taactctgcg
     1861 cccaacgccc gctgtttatg ttctgcggta acgatatccg ccatcagccg atccacccgc
     1921 gcctgataac gcgtcgggtc cagtcgaaat agcacctcac cttttttaat cagcgtattt
     1981 ttcttatccg tcacttcgat caccacgcct gtcacctgtg ggacaacagg aatagaaata
     2041 accgcttttt gcgctttgaa ggtatacgga tggttatagt tcattaacag aattaaacca
     2101 ctgacaataa atatgccacc cagggccgct gtggaaatgg tccatttatt tacaggaatt
     2161 ttgaagatct taaacattgc ccatgcaaaa gccacatagg tcaaaataat cagtaaatcc
     2221 ataagtatta ctcggcagaa gaggatttga tgtcggcgag ctgtttctcc agcccggcaa
     2281 tacgctgttg tagttgcaca acggaggagt catggtttta cattccccat ccccgctccg
     2341 ggcggtaaag cgtggcccaa atccacaaaa atggccagat aacatgcagc gtaaaaagac
     2401 tcacccaacc agcaacatga atggcgtcgg catgaggatg attacgtttt ttagcaatca
     2461 ggtagggaat atcatgtaaa ataataatcc cacagaatat caccagaaat acaaaaataa
     2521 gcactcccag tgcaaaatag tccaggaaca tatttccctc aaagaatata aaaaagaaca
     2581 attaacgcat attatgaaat gccatgatgc agtgcaattt ccttatttat ataacgacat
     2641 attgtttttt atatactttc tgaaatgtgc tgtctggttt ttcaacagga cgaggtttat
     2701 catattgaaa tagtggaact attagccaaa ctaatgaata actccagtaa aacatccaca
     2761 gtacagatta agcgtattaa accttcaatt atctaccgtt tattgctgat tggcctcgga
     2821 tcaccaatgg tgatttacgg cctggttcgc ccgctcacca tcgaaacgcg agattaaact
     2881 cctgacaaaa cacgccccag aaacgcactt ctgttggcgt agtctgggtt attgcgcacg
     2941 taggtttaaa acgtaaattg taacaacgtg cgctttgttt atgccggatg cggcgtgtac
     3001 gccttatccg gcctacggtc aggttcccgt aggcatgata agacgcgtaa gcgtcgcatc
     3061 aggcaatgaa tacccaatgc gaccagcttc ttatatcaga acagccccaa cggtttatcc
     3121 gagtagctca ccagcaggca cttggtttgc tggtaatgct ccagcatcat cttgtgggtt
     3181 tcgcgaccga tacctgattg tttgtagcca ccaaacgcag catgtgccgg gtaagcgtga
     3241 taacagttgg tccacacgcg cccagcctgt atgccgcgcc ccatcttata ggccagatta
     3301 ccgttgcggc tccagacgcc cgcgcccagg ccatattgcg tatcgttcgc cagctccagc
     3361 gcttcttcca tcgttttgaa ggtggtcacc gccagcaccg ggccaaaaat ctcctcctgg
     3421 aacacccgca tattgttctg accaaacaga atcgtcggtt cgaggtagta gccgtctttc
     3481 agttcacctt ccagcagctt gcgccgcccg cctgtgagca cgtcagcgcc ctctttttta
     3541 ccgatatcaa tgtagttgag gatggtttcc agttgcccgt gagaaacctg cgcgcccatt
     3601 tgcgtcacgc tgtcgagcgg gttaccgctg cgaatgcttt cgacacggcg gatggcgcgt
     3661 tccataaagc gttcgtagat tgactcctgc actaaggcac gactcggaca ggtgcaaact
     3721 tcgccctggt taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa aaaggcatct
     3781 tcttcatcca tcacatcagc aaagaagata tttggcgact taccgcccaa ctccagcgtc
     3841 accggaataa tgttttgcgt tgcgtattgc ataatttgtt ggcccacttc cgttgagccg
     3901 gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat attcgccaat ttccccacct
     3961 gcgccattga ccacgttcac cacgcccggc ggcagtaaat caccgacaat ttccattagc
     4021 agcagtacag aaagcggggt aagacgtgcg ggtttcagca ccacacagtt gcccgccgcc
     4081 agcgcgggag ccattttcca gctcgccatc agcagcggga agttccacgg gataatctgc
     4141 cccaccacgc ctaacggttc atggaaatga taggccacgg tttcgctatc aacttcactg
     4201 atcccacctt cctgcgcccg aatacacgag gcgaaatagc ggaaatggtc aatcgccagc
     4261 ggtacatccg cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt ttcagctgtc
     4321 gctaacagct cgaggttttg ttccattcga tcggcaatct taaacagaat cgccgcgcga
     4381 tcctgcaccg aggtgtgcgc ccatttatct ttcactttgt gcgcagcatc cagcgccaga
     4441 tcgatgtctc gtttgcccga agacgccact tcgcacagca gctgcccggt caccggcgtc
     4501 aggttctggt aatactcacc atcggcagga gctacccatt cgccgccaat aaagttgtcg
     4561 tagcgggctt ttaacttgat ggggaaacca tactcgccgg gcttaatctg tgctgaaggg
     4621 ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta gacgtgactg
     4681 gcgaaatctt cgccagtcgg taacagcttt acgacagcta tcacgaattt acgggcaagg
     4741 attacagaga tagttgaaaa agcggctgac aatttgccag ccgttgttgg aatgatgatt
     4801 acattgcggc gcgataaatc gccacaattt cttcgtgagt tgcctggatc gggttagtaa
     4861 agccacaggc atctttcagg gcattagtcg ccagaaccgc gaaatcttct tctttcacgt
     4921 tcaggtcgcg taggcctgcc gggatatcca ctttcttcgc cagttcacgg atggcgttaa
     4981 tgcaggcttc agcaccttcc gcgtcgtttt tacctgtcac gttcacgccc attgcagcgg
     5041 cacagtcacg cagacgtgca gcggcgactt tgctgttgaa tacctgaaca tgcggcagca
     5101 gaacggcgtt acatacaccg tgtggcaggt tgtagaaacc gcccagctgg tgcgccatcg
     5161 catgaacata acccagagaa gcattattga acgccatacc ggcaaggaac tgggcataag
     5221 ccattgcttc acgcgctttc gcattactgc catcttcaac ggctaacggc aggttttcgg
     5281 caatcatggt cacggctttc agtgcacaag cgtcagtgat cggcgtggcg gcaatagaaa
     5341 catacgcttc gatagcgtgc gttaaggcat ccatacccgt tgcggcggtc agtgacttcg
     5401 gcataccaat catcagagag gagtcattga cagaaagcag cggagtcacg tgcttatcaa
     5461 caatcgccat tttgttatga cgcgcttcgt cagtgatgat gcagaaacgg gtcatttcag
     5521 aggccgtacc cgccgtggta ttgatggcga tcatcggcag ctgcggtttt gcagagcggt
     5581 caacgccttc gtaatcgcga atatcgccgc cattggctgc caccagcgca ataccttttg
     5641 cgcagtcgtg tggagaacca ccgcctaagg agatcacgct atcgcaatta ttttctttaa
     5701 gtaatttcaa acctgcggcg acgttttccg tggtggggtt aggttgggtg ccatcataaa
     5761 taacgctaaa aatattgcgt tcttccagtg ctttttgcac atcacccgcc atacctaatt
     5821 tcgttaacat attgtcagtg acaattaagg tacgggtaaa tccataatct gccatcatat
     5881 tcattgcatc agtcaatgaa tcagcgccga tgacattcac tgaaggaata aagaaagttg
     5941 aagatgccat aacacactcc tgaaagtgta aaaaagggtg ggcagcttac tgaggatttt
     6001 cattcgaaaa catgatcatt attattaatt atcaaaatta atctaataaa aagcacattg
     6061 tattcattaa acaatgtgct ttatttaaaa tcataagaaa tttgtattaa tttattttaa
     6121 aaattaatta atcatttcct tatttttccg gaaataataa tgcgtcgcgc aataaatgat
     6181 catttccacg acgacgcgta aagccaatgc ggtcaaaata ttccagaatc tgaattccca
     6241 gctttcggcc tacgcctaag cgatcgcgga aatccgccgc gcaggttgaa ccacactcct
     6301 gatcgagatc gcggatcata ttggcaaatt cgacaatccg atcgttacgg taataacgat
     6361 ctttgacgat cgcggtaatt attccttgct gcgccgcctg gcgtagcgtc aggcgcattg
     6421 cctgctcgtc ggtacccgtc tcttttgcca gatcacgcac ccaccacggc tcgtcaccaa
     6481 acagcggctc tgctttttgc cagatggcct gctgctcttc gctgaagccc gctttgtgat
     6541 ccggcagatg cagccaaccg tgatgactga ggatgtcgcc gctttcgcgc atcttttcaa
     6601 tcagcaacag tactagcgct tcatcttcca ttggcaacgc catacgtcgc agacgttcgc
     6661 gcccaggccc aggttcatcg cgatgttgct catgataagt cgctaatgtg tcgagaattt
     6721 tccgttgcca gcgagcggca accggcgcat tcaacaagct ataaccagcc tgaatatagc
     6781 caggctgttg cagcagttcg cgcatccctt cgccgttgag ctggcgcgcc caggcgaaat
     6841 caggaagatt aaccgcgccg cgttgcaggt gaacggctaa cgcttcgcta tcattctgtg
     6901 ccctggcgag ggaagccagc cattgcagat actctggttt acgtttaccg cgacgcggcg
     6961 ggttaagcat cacgacgcgc gctccggcca gcgtgttgcg ggcagagata tcgcgcaata
     7021 ccaggcggtc gttatctgcc agccataacg gggtgtcgaa gaccagttca gcaaggttat
     7081 cttccagcag tgaaacgcgt cccgtgacgt ggctggcggc gtggtgaata tgcagcggct
     7141 gccactgggt cagcggtgta tgggtttgaa gctcgacaat cacccgtgtg aacggctctg
     7201 gcggcacatc ggcaagcagc cagtcgccac ggttaatctg ctctttttcc gcatcacccg
     7261 cgatgtcaag cgcgatacgc tgcccagcat tggcggtttc tgttggctgg ttttgcgcat
     7321 gcagcgcacg tacacgcatc ggtttattta caccagtcag ccagagtgaa tcgcctacct
     7381 tcacttcccc gcttaacgcc gtaccggtga cgaccagccc ggcacctttt acggtaaatg
     7441 cgcggtcaat cgcgaggcgg aaactatgtt ggctggcgtg ctcgcgttcc ggcaactgaa
     7501 gcagatgctc gcgcagggca tcaattcccc gaccttcggt tgctgcggtg ataaacagtt
     7561 ttgcctcagc aaaaccgtat tcccgcagaa cctcctttac ctggcgttca acctcatcaa
     7621 cacgcgcttc gtccacgcga tcggctttgg tcagcgccac tgtcagcatc gggttaccgg
     7681 tcagctgcaa aatcgccaga tgctcacggg tctgtgccat cacgccgtca tcgcacgcca
     7741 ccaccaacag cgcgtgatcg ataccaccaa cgcccgccag catgttggaa agaaactttt
     7801 catgaccggg aacgtcgata aaaccaggca cgcggccatc cggctgcggc cagtaggcat
     7861 agccgagatc gatggtcatg ccgcgctttt tttcttccgg cagacggtca gcatttacgc
     7921 cagtaatcgc ctgcaataag gttgttttgc cgtggtcaac gtgtccggca gtcgcaataa
     7981 tcatttcaac aacatctcca aaaaccgttg ctcatcttca aggcagcgta aatccagcca
     8041 caatcgtccg tcataaatac gaccaatcac cggcactggc aattcacgcc agcgggcggc
     8101 taatgactca aggtggctac cgcgtccatc atggggtgta aacgttaatg ccgcgctcgg
     8161 caggcgatca accggcagcg aaccactgcc aatctgcgaa agacatggca taacctgtac
     8221 cgcaaactcc gcgccgtaat gtgcggcaag gggagcctgt aaacattgtg cctggatttg
     8281 aatgacctct gcgctgcggg taagcaggcg cagggtcggt aatttttcac tcagagcttc
     8341 agggtgtaaa taaagacgca acgtggcttc cagcgccgcg agggtcattt tatccgcgcg
     8401 taatgcacgc ttcagcgggt ggctttgcag gcgggcgatc atctcttttt taccaacgat
     8461 aatccctgcc tgcggcccgc ctaacaactt gtcgccggag aagctcacca gactgacgcc
     8521 cgccgcaatc aactcctgcg gcattggctc tttcggcaaa ccgtactggc taagatcgac
     8581 cagcgagcca ctgcctaaat cagtcaccac tggaacatcc agctctttgc cgagcgccac
     8641 cagttcagct tcgtctatcg ctttggtgaa cccctgaatg ctgtagttac tggtatgtac
     8701 tttcatcaac agtgcggtat tttcattcac cgcctgacga taatcattcg cgtgcgtgcg
     8761 gttggtggtc cctacttcgt gtagggtaca gcctgcctga cgcataacat cgggaatacg
     8821 aaacgcgccg ccaatctcca ccagttcgcc gcgagatacc accacctctt tcccgctggc
     8881 agtggccgcc aacatcaata acaccgccgc cgcattgtta ttgacgatac aggcatcttc
     8941 cgcccccgta atacggcaca gcagctgcgc cagcgcccga tcgcgatgtc cgcgtccggc
     9001 gtcgtccaga tcgtactcga gggtcactgg cgaacgcata gcctgcgcaa cggcttccac
     9061 cgctgcttcc gcctgtaaag ctcgcccaag gttggtatgc agcacggttc ccgtcaggtt
     9121 gatcaccgga cgcagcgcgc tctgcgcctc tttcgtcaac cgggcatcga cttcttgcgc
     9181 ccagttttca caccacgcag gcagcgtctg gctgccacga atcacttctc gcgcttcgtc
     9241 gagcatctga cgcaacaatt ccaccacgcg ggtgtgacca taagtatcac gcaaagaaag
     9301 gaaggagcta tcgcgcaata agcgatcaat agccggaagt tgactataga gggaacgcgt
     9361 ttcggttgtc ataggaaacc tggctgatca aggccctctc acacggagaa gggcgtttaa
     9421 cataaccacg gattgtaacg tgagatgggt caggaggaca tatcgcgcat caagcctttg
     9481 gcggtttggt gcaagcaaaa ctttcgcggc taaacaggtt ttcgaccagt ttgactaagt
     9541 gtgggcggtc gacacaccaa cctggcgcaa cgcggcggaa attgagatat ccgacagcac
     9601 aggcaatggc gatagtcgcc agattgaccg tatcggtttt gagtgtgcca tcgaccagat
     9661 atccttccag cacatccaga ctgcggttga ttttctcccg ctggcgtaac aattcatctt
     9721 cagactgctg cgccgccgga cgcgcctgtt cacgcaccga taccagcccg gcatccataa
     9781 tgccatccgc cagcgcctca attttgcgca cccgcaacga ctccagcgga tcgcgcggca
     9841 acatcgccgg agcgacattc attaactcaa tatattcagc gatgatcggc gaatcaaacc
     9901 agcattcgcc ctcttcggtc accagcacca gcactttccc taacgggtta aattgcgcca
     9961 cgccgttatc cgcgttatag ggcagttcat taataaattc gaaagttatg cccttttcta
    10021 acaacagaat agaaagtttg cgtacaaacg ggctggtgta gctaccgacg agtttcatgc
    10081 cgagtccttt gtgcgaggaa aaatatcagt atggctcagg ctcacgcaaa acacaggtga
    10141 tataagtctc aatgatccaa ataaagataa tgcatccaac tggtcattct tagcagcact
    10201 ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg ggcgtagatg
    10261 ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc cagcacaccg
    10321 tccgtgccgg gaacgaccgt taatgattgc aataccccct gcgcctgata agaaccgcct
    10381 ggcacgacag gtaaaatact agtcaatttg ccgtggaaca cctgcccagg tagcgcgtta
    10441 aacaccactt ccgcatcatc gccaggtttc agacgtaaca gcgagttttg ccgaaattgg
    10501 gcaacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag cggcaaggca
    10561 gctgcgtatg tacctgggcg gatcagtacc tgagtgacgt agccattgct tggtgcgcga
    10621 atgacagtct gctcaagatt atattttgct tcagtaagtt gcgctcttaa gctcacaatc
    10681 tgcgattgct cgccgttgac catactgtcg agctgactct ggatctgcgc ctgctccgcc
    10741 accgagcctt tcactaacgc atcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca
    10801 cgttccgaga acggattcac cgccgcctgg ctgcctttca ggtaacgttg ataattttta
    10861 aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc
    10921 gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact
    10981 cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg aataagctga
    11041 ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat agggatcgct
    11101 atcactgcct tttgcgcggt aaaagtgtaa aggtggttgt agttcatcaa caaaatcaaa
    11161 ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgccacgta agttaaaaca
    11221 atcaatagat ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg
    11281 cgagttgatg ctccagccgg gaaattcggt cagaaagtgc ggcgatttcc ggttcagtcg
    11341 ctttctcctg cgacgcaaca tgtgactgca taccccagcc acgctccggt tgatagagcg
    11401 tcgcccagat ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg
    11461 ccgtatgaat agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt
    11521 catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg
    11581 cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg ttttttggct
    11641 ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc
    11701 ataacaccaa agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg
    11761 acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga
    11821 ttcagatcac aaagattcaa caaaccatca aaacaaaaat gtgacattac tcacatttaa
    11881 atgccatttt tagcgaaaat caccgccttg tttctttttt acacaggcgt tttgtgatga
    11941 gcgtcacgtc aattacctct ctacccccta tatttatgtg actgatatca cacaaaagcc
    12001 cgtcgactgg acagttaacc gattcagtgc cagatttcgc agtctctaca aggtccggct
    12061 acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata
    12121 agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc
    12181 ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg
    12241 ttatttattc caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg
    12301 atcacttatc tcctaccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa
    12361 cgtggcggcg tagtcggtgc cattaccact atgggcgtta tcgtcggcgc agacatgccg
    12421 atgttcctcg gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac
    12481 cgctgggtag acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca
    12541 ggcatcatcg ggatgatcct cgctattctg gcattcctcg gcattggccc gattgttgaa
    12601 gccctgtcca aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg
    12661 ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac
    12721 ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc aatcttcttc
    12781 ctgattgaag ctaacccagg tccaggtatg ggcgtgcttt gtgctggaac gtattgctcc
    12841 ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat
    12901 catcgcctgt gaaaacatgg tacgcggcac cacgcagctg aaaggtcatg tgatgaacgc
    12961 cctgccggaa gacgccaaag cgtgggtaga agagcacgtt ggctttgtcg attccgccgt
    13021 tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga
    13081 aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc
    13141 aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa
    13201 cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga
    13261 cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc
    13321 ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat
    13381 cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc
    13441 gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata
    13501 cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga
    13561 agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc
    13621 gctggcacag atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc
    13681 ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga
    13741 ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag
    13801 cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc
    13861 gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat
    13921 gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa
    13981 ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg
    14041 gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc
    14101 cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa
    14161 ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca
    14221 acaatggtcc tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa
    14281 aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa
    14341 tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg
    14401 ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct
    14461 acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg
    14521 tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc ggtaaagatg
    14581 acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttc
    14641 tatagatttc ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt
    14701 gcagaactat tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc
    14761 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
    14821 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
    14881 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
    14941 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
    15001 ccgttccagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
    15061 gttcgcggac tggtggttgt gcacccaatg accgcgctga ggcgcgaaat gggcctggaa
    15121 gagatgactg gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta
    15181 tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct
    15241 aattaccaaa caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg
    15301 catttttacc gaaatagcca tctataaaca ataaaaatat atagaagata tcatttgtta
    15361 attaacaag
//
LOCUS       DADKXM010000092        15336 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.093, whole genome shotgun sequence.
ACCESSION   DADKXM010000092 DADKXM010000000
VERSION     DADKXM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.093"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="JQR05_002763"
     CDS             complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="JQR05_002763"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY5324893.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEA"
     gene            complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="JQR05_002764"
     CDS             complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="JQR05_002764"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY5324894.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="JQR05_002765"
     CDS             complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="JQR05_002765"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY5324895.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            3213..5759
                     /gene="cyaA"
                     /locus_tag="JQR05_002766"
     CDS             3213..5759
                     /gene="cyaA"
                     /locus_tag="JQR05_002766"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY5324896.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="JQR05_002767"
     CDS             complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="JQR05_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY5324897.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(6167..7480)
                     /locus_tag="JQR05_002768"
     CDS             complement(6167..7480)
                     /locus_tag="JQR05_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY5324898.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(7546..8942)
                     /locus_tag="JQR05_002769"
                     /pseudo
     CDS             complement(7546..8942)
                     /locus_tag="JQR05_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9131..9334
                     /locus_tag="JQR05_002770"
     CDS             9131..9334
                     /locus_tag="JQR05_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5324899.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9371..10195
                     /gene="dapF"
                     /locus_tag="JQR05_002771"
     CDS             9371..10195
                     /gene="dapF"
                     /locus_tag="JQR05_002771"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY5324900.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            10192..10899
                     /locus_tag="JQR05_002772"
     CDS             10192..10899
                     /locus_tag="JQR05_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY5324901.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLVRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10896..11792
                     /gene="xerC"
                     /locus_tag="JQR05_002773"
     CDS             10896..11792
                     /gene="xerC"
                     /locus_tag="JQR05_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY5324902.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     QQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11792..12508
                     /gene="yigB"
                     /locus_tag="JQR05_002774"
     CDS             11792..12508
                     /gene="yigB"
                     /locus_tag="JQR05_002774"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY5324903.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12592..14754
                     /gene="uvrD"
                     /locus_tag="JQR05_002775"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12592..14754
                     /gene="uvrD"
                     /locus_tag="JQR05_002775"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY5324904.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14901..15332)
                     /locus_tag="JQR05_002776"
     CDS             complement(14901..15332)
                     /locus_tag="JQR05_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324905.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3566 a         3973 c         4102 g         3695 t
ORIGIN      
        1 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca
       61 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
      121 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
      181 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac
      241 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
      301 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
      361 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
      421 aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
      481 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
      541 gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
      601 tcgggtcatt catatccgcc aagctggcgt ctgcactttt cagcaacgct gcagaggtcg
      661 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
      721 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat
      781 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt
      841 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
      901 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
      961 catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     1021 cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     1081 ccctagtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     1141 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     1201 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     1261 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     1321 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     1381 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     1441 caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     1501 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     1561 cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     1621 tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct
     1681 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     1741 gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     1801 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     1861 gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag
     1921 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     1981 atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     2041 cagcgcacgt agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg
     2101 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     2161 gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat
     2221 accgactgca ccttgtccta ccgccggaag agaaatttcg ggtggtaacg cggcgcgaat
     2281 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     2341 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     2401 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     2461 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     2521 ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt
     2581 cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac
     2641 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     2701 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     2761 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     2821 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     2881 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     2941 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     3001 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     3061 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     3121 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     3181 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     3241 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     3301 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc
     3361 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     3421 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     3481 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     3541 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     3601 gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtag
     3661 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg
     3721 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg
     3781 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     3841 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     3901 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     3961 agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag
     4021 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     4081 acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta
     4141 ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct
     4201 atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag
     4261 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     4321 accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga
     4381 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     4441 ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc
     4501 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     4561 cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg
     4621 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata
     4681 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     4741 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     4801 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc
     4861 gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg
     4921 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     4981 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     5041 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     5101 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa
     5161 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     5221 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     5281 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     5341 tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac
     5401 cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc
     5461 aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc
     5521 accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat
     5581 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     5641 agattgtgaa ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca
     5701 acctgccgcc cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat
     5761 gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     5821 cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     5881 caaatccatt catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac
     5941 acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg
     6001 gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga
     6061 cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt
     6121 gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     6181 tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc
     6241 tgttttgaac ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg
     6301 gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc
     6361 gatcgttttt agttagcgga ccgttaactg gaataatttg caagctcttt gaccgtaatt
     6421 ggcggataaa gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg
     6481 gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat
     6541 acagccgatc tttatattgc gcaatattgc cagtaatcag atggaattgt tcaggttcaa
     6601 tacctggtag gtctatccac ttgctccact gacggtcggg cgtgagctgt ttacgccagc
     6661 gaacgcgact ttcccctact tcaaaagaga cgtcatcatc tacgggtatc gctttgcctg
     6721 ataattgacc aaagggataa cgatgcagac ccttattgtc gcgaaaaacc agcagtgaat
     6781 gaggtatcca gcgaattatc tcaaatgagt ctgcattcgc cgtaaggtga acgccattta
     6841 aataaatatt ctgcccactg cggataagca gatcattgga atatgaattt ctgtttacac
     6901 tcataagacg tggattaata ccccaccatc ttttttctaa tacagtaaca tcactgctgc
     6961 tcccctgacg tcgaccggac aagtagagat ttttactatc caccagcgtg gtagagttac
     7021 cttcgatagc ttttagtgtc gccagatcaa cacgtgacgc gccgctatta ctttcggtgc
     7081 gctgtccgtc aaaatagagg ctatatttat cgaccgcgaa gcggcccata gcctgaaagc
     7141 tggcaatatc gacagtgggt gttcccggtg gattgctaac gattttgcca cgccagagaa
     7201 taacgcggcc gtctgtcacc cagtggtatt cctcattttc tatgcttatt tcatcggaag
     7261 gtatataacc cgttttccca gagatatttt tcacaaggcg caaatcggcc ctgctcatct
     7321 ccggaaactg ggcaatgatt ttcttatccc cccaataacc tttaccgtaa gacataacac
     7381 cgtgatcatt gtaatagtcg aaccctatgg cctgtacagc aggcgtcaga aatgtgtgtc
     7441 ccaacaggat gagtgcaact gtgattattt gacccttcat tttgtcgcct cattgcgcgg
     7501 ggcaggaata aattcgccgt catcagtaat ttttccgtcc agatatcacg ttttgtaatt
     7561 tacgccatag ccatatcccg aatgttgttc cttccagatt aagcgaactt gtttcggatc
     7621 agcggtgcgg tatcgccaga gctgcgcgcc atcaaagctg taaatgtagc ggttatcgcg
     7681 ggcataccat ctgcgcaaaa agggatggtc attaagatgg gcttcgcgtt catccggaga
     7741 acggttatcc agtaagatca gcggtccgtc agattcaaac acgtgtaaac cggaatggta
     7801 tggccactct tcggcacgcg taagtaaata gccgtggcgt ttcccggcgt tgaaacgttt
     7861 gttgataata agattgggat catcaagttt cacgatctcc agcttacggt caccagactc
     7921 tgtttcgatg atggtataaa gcgagtcctg atactgggcg acagcatcac tgatgggttg
     7981 aaactgctcc ggatcgagtc cgggtaattc ttcctgttta caagcaggtc ctttacgcca
     8041 gaacactttc ttttccagca gagaaaaatc cagacagtgc tttgttaaat cttcatccca
     8101 cgccagattt tccttgtcga ggactttacg ctgcagcccg tttttatcac gccaggtcaa
     8161 gaatgagccg ggcatccagc gcacgacgga aaaggtgtcc gggtcggcat caagctgttc
     8221 gccgttgagc aggattttgg tccgtgtacg agccagggta tcccaggggc caaatggtac
     8281 ggcaatgcag gggttaaacg tggtggaaaa ctttcctcgc tgatcccagg acttctgcgc
     8341 cagcacacgg aaactctcgg ggtcagcgag gcgatgccca ttgatataca agaaattagc
     8401 gtcacgtaat atcagcccca gtaagtcagg atcccaggga ggccggaatt ccacctgatg
     8461 cagcgttttg atatccaagc tatttccacc accattgtca tcggtgcgct tgccttcaaa
     8521 atagagactg tgtttatctg cggcaaaatc accccatgcc ttgaacgagg cgacatcgac
     8581 gggaggtgtg ccgggagggt tttgcacaac ttcacccgcg tagaggatat tgcgaccatc
     8641 gctatgccat gaataatgag gataccaggc ctgtgcgccc tcatagaggt agtcggttgt
     8701 caaatcagtc agaggcggtt ctttatccca ttcgtctagt gttgtcgggt ctattaataa
     8761 attgggtaac cgacgcaaac tcttgtaatt cacgtcaggt agagaaagat ttgtttcttt
     8821 ataagggcgc aggtacacca cgccttttcc ctgtttctgg taaatattat gagccgacat
     8881 ccccatgccg acgataaaaa caatgaacaa gccaacaaac aaaccacatt gcgatagtgc
     8941 ataaagccat cctggcgcga ggtgttgatc acgaaactgc cagcagaaca taaatcctca
     9001 cgagtaagcg ttatactcgc agcatttcct cacttttcag acttcataaa gagtcgctaa
     9061 acgcttgctt ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag
     9121 gcaatccata atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct
     9181 gacgggctgc ggtctgaaag gtccgctcta tttcccgcct gcagataaaa acgcaccgcc
     9241 gccgaccaaa ccggtagaga cgcaaacgca atccacggtg ccggataaaa acgatcgcgc
     9301 cactggcgat ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt
     9361 ggagtaaatg atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga
     9421 cgcggtaacg cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg cggatcggca
     9481 cctgggggta gggtttgacc agctgctggt ggttgagccg ccgtatgatc ctgaactgga
     9541 ttttcactat cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gtaacggtgc
     9601 gcgctgcttt gcccgttttg tgcgtctgaa aggactgacc aataagcgtg atatccgcgt
     9661 cagcaccgcc aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa
     9721 tatgggcgaa cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa
     9781 gacctatatt atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa
     9841 tccgcattgc gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc
     9901 tgttctggaa agtcacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt
     9961 taagcgcgag catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg
    10021 cggcagcggc gcgtgtgcgg cggtggcggt agggattcag caaggtttgc tggacgaaga
    10081 agtacgcgtg gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc
    10141 gttatatatg actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa
    10201 ccaggggaag aactgcagga aacactcacg gagcttgatg accgggcggt tgtcgattat
    10261 ctgattaaaa atcctgagtt ttttatccgc aatgcgcgcg cagtggaagc gatacgtgtg
    10321 ccgcatccgg tacgcggcac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat
    10381 cacattcatg ttctggaaga gaacatggcg ttgttgatgg agcaggctat cgccaacgaa
    10441 gggctgtttt atcgcctact ctacctgcag cgcagtctca ccgccgccag cagtctcgac
    10501 gatatgctgg tgcgctttca ccgctgggcg cgcgatctcg gcctggctgg tgcgagtctg
    10561 cgcctgtttc cagatcgctg gcgcttaggt gcgccgtcga accacactca tctggcatta
    10621 agccgtcagt ctttcgaacc gctgcgtatt cagcgtttgg ggcaggaaca gcactatctt
    10681 gggccgctta acggaccgga gctgctggtg gtgctaccgg aagcgaaagc ggtgggatcg
    10741 gtggcgatgt cgatgctggg aagcgatgct gatttgggtg ttgtgctgtt tactagtcgc
    10801 gatgccagtc actatcaaca agggcaggga acgcagttac ttcatgaaat tgcgctgatg
    10861 ttgccggagc ttctggagcg ttggattgaa cgcgtatgac cgatttacac accgatgtag
    10921 aacgctacct acgttatctg agcgtggagc gtcagcttag cccgataacc ctacttaact
    10981 accagcgtca gcttgaggcg atcatcaatt ttgccagcga aaacggccag caaagctggc
    11041 aacaatgcga tgcagcgatg gtacgcaatt ttgctgtacg cagtcgccgt aaagggctgg
    11101 gagcagcaag tctggcgtta cggctttctg cgctacgtag cttttttgac tggctggtca
    11161 gccagaacga actcaaagct aacccggcga aaggagtttc ggcaccgaaa gcgccgcgtc
    11221 atctgccgaa aaatatcgac gtcgacgata tgaatcggct gctggatatt gatatcaacg
    11281 atcccctcgc tgtacgcgac cgtgcaatgc tggaagtgat gtacggcgcg ggtctgcgtc
    11341 tttccgagct ggtggggctg gatatcaaac acctcgacct ggagtccggc gaagtgtggg
    11401 tgatggggaa aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga
    11461 ttgagcactg gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt
    11521 cgaaactggg caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca
    11581 taaaacaagg gctgaataat cacgttcacc cacataaatt acgtcactcg ttcgctacgc
    11641 atatgctgga gtcgagcggc gatcttcgtg gtgtgcagga gctactgggt catgccaacc
    11701 tctccaccac gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg
    11761 cggcgcatcc acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat
    11821 ctcggcgctc acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg
    11881 caccgagcga gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca
    11941 gaatgaagat ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca
    12001 cgacgtgacg cgttggcgtt ttcgttcgat tgaacaagcg atgctcgacg ccgggctgag
    12061 tgccgaagaa gccagtgcag gcgcacacgc agcaatgatt aactttgcca aatggcgcag
    12121 ccgaatcgac gtcccgcagc aaactcacga caccttaaaa cagctggcga agaaatggcc
    12181 gctggtggcg atcaccaacg gtaacgccca gccggaactg tttggtttgg gggattattt
    12241 tgagtttgtg ctgcgcgctg gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt
    12301 tttggctgcg gaaaaactca acgtgccgat cggcgagatc ttacatgttg gggacgatct
    12361 caccactgac gtgggtgggg caattcgcag cggaatgcag gcttgttgga tcagaccgga
    12421 aaatggcgat ctgatgcaaa cctgggacag ccgtttactg ccacatctgg aaatttcccg
    12481 gttggcatct ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg
    12541 gcggagggcg ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt
    12601 tcttacctgc tcgacagcct taatgacaaa cagcgcgaag cggtggccgc gccacgcagc
    12661 aaccttctgg tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc
    12721 gcctggctga tgagcgtgga aaactgctcg ccatactcga ttatggctgt gacgtttacc
    12781 aacaaagcgg cggcggagat gcgtcatcgt atcggacaac tgatgggcac cagccagggc
    12841 ggcatgtggg taggcacctt ccacgggctg gcgcaccgcc tgctgcgtgc gcaccatatg
    12901 gacgccaatc tgccgcagga tttccagatc ctcgatagcg aagaccagct acgcctgctt
    12961 aagcgtctga tcaaagccat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg
    13021 tggtacatca acagccagaa agatgaaggc ctgcgtccgc atcatattca aagctacggt
    13081 aatccggtgg agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgcgatcgc
    13141 gcgggcctgg tggacttcgc cgagctgctg ctgcgcgctc acgaactgtg gcttaacaag
    13201 ccgcatatcc tacaacacta ccgcgaacgt tttaccaata tcctggtgga cgaattccag
    13261 gataccaaca acattcagta cgcgtggatc cgcctgctgg cgggcgacac cggcaaagtg
    13321 atgatcgtcg gtgatgacga ccagtcaatc tacggctggc gcggggcgca ggtggagaat
    13381 attcagcgtt tccttaatga tttccccggt gccgaaacta ttcgtctgga gcaaaactac
    13441 cgctctacca gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt
    13501 ctgggtaaaa aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct
    13561 tttaacgaac tcgatgaagc gcgttttgtg gttaaccgca tcaaaacctg gcaggacaac
    13621 ggcggggcgc ttgccgagtg cgccattctc taccgcagta acgcccagtc gcgtgtgctg
    13681 gaagaagcac tgttgcaggc gagtatgccg taccgtattt acggcgggat gcgcttcttc
    13741 gaacgccagg aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgtaacgac
    13801 gacgcggcct ttgagcgtgt ggtgaatacg ccaacgcggg gtattggtga ccggacgctg
    13861 gacgtggtac gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa
    13921 ctgttgcagg aaaaagccct cgccggacgt gctgccagcg ccttacagcg gtttatggaa
    13981 ctgatcgacg ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg
    14041 gtaattaaag actccggcct gcgtaccatg tacgagcagg agaagggcga aaaaggtcag
    14101 acgcgtatcg aaaacttaga ggaactggtg acggcaacgc gccagttcag ctacaacgaa
    14161 gaagacgaag atttaatgcc gctgcaggcg ttcctatccc atgcggcgct ggaagcaggc
    14221 gaagggcagg cggatacctg gcaggatgcg gtacaactga tgaccatgca ctcggcgaaa
    14281 ggtctggagt tcccgcaggt gtttatcgtc ggtatggaag agggcatgtt cccaagccag
    14341 atgtcgctgg atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta
    14401 acccgcgcga tgcagaaact gacgctgacc tacgcggaaa ctcgccgtct gtatggcaaa
    14461 gaggtttacc atcgcccgtc gcgctttatt ggcgagttgc cggaagagtg tgtggaagag
    14521 gtgcgcctgc gcgccacggt aagccgcccg gtcagccatc agcggatggg tacgccgatg
    14581 gtcgagaacg acagcggcta caagctcggc cagcgtgtac gccacgctaa gtttggtgaa
    14641 ggcaccatcg tcaatatgga aggcagcggt gaacatagcc gtttgcaggt ggcattccag
    14701 ggccagggaa tcaaatggct ggtggcagct tacgcccggc tggagtcggt gtaacgttgc
    14761 cggatgcggt gctgcgcacc ttatttggcc taaaaaatca ttcagattca ataaattgca
    14821 acgtcatgta ggccggataa ggcgtttacg ccgcatccgg caccagcagc atatttagta
    14881 tctgacacaa agctatcatt ctaacctttc cgctcgacgg aaatcatggt gacaaaagga
    14941 tagcgttgcc acgggattgc cccgcctttc atatacatat gtgaaatcgt gccatcaaga
    15001 taaagcagct gctcaacgtt cagtttcgct ttggcataac aggcaaaatc ataaaaattt
    15061 gtcgcctgct ggctcaacaa aaacacggca ttcccatgtt tattaatccc aacaccgtta
    15121 cgaattttac gtgaggcgac gttgggatga atacgcggat taattacacc gttttccatc
    15181 aacattggtc ctgactgcac cgcaaactga atatctttac tggctttaaa agcatccaga
    15241 cgaacgatgc cgactttatc tcccgcgaca taaaacacgc cgccaggacg gataaagaaa
    15301 ttcccttcac ctgaagcgag atttaacgcc accttc
//
LOCUS       DADKXM010000093        15262 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.094, whole genome shotgun sequence.
ACCESSION   DADKXM010000093 DADKXM010000000
VERSION     DADKXM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.094"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..606
                     /locus_tag="JQR05_002777"
     CDS             <1..606
                     /locus_tag="JQR05_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY5324906.1"
                     /translation="LPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVDQ
                     TTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMVM
                     GAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSI
                     IAALLAYRILYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(642..1355)
                     /locus_tag="JQR05_002778"
     CDS             complement(642..1355)
                     /locus_tag="JQR05_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY5324907.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPSSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAITITLVWQRHTRFFH"
     gene            complement(1560..2264)
                     /locus_tag="JQR05_002779"
     CDS             complement(1560..2264)
                     /locus_tag="JQR05_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY5324908.1"
                     /translation="MDRFPRSDLIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(2401..2853)
                     /gene="moaE"
                     /locus_tag="JQR05_002780"
     CDS             complement(2401..2853)
                     /gene="moaE"
                     /locus_tag="JQR05_002780"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY5324909.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(2855..3100)
                     /gene="moaD"
                     /locus_tag="JQR05_002781"
     CDS             complement(2855..3100)
                     /gene="moaD"
                     /locus_tag="JQR05_002781"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY5324910.1"
                     /translation="MIKVLFFAQVRELVGADATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(3093..3578)
                     /gene="moaC"
                     /locus_tag="JQR05_002782"
     CDS             complement(3093..3578)
                     /gene="moaC"
                     /locus_tag="JQR05_002782"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY5324911.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(3581..4093)
                     /gene="moaB"
                     /locus_tag="JQR05_002783"
     CDS             complement(3581..4093)
                     /gene="moaB"
                     /locus_tag="JQR05_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY5324912.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREFEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(4115..5104)
                     /gene="moaA"
                     /locus_tag="JQR05_002784"
     CDS             complement(4115..5104)
                     /gene="moaA"
                     /locus_tag="JQR05_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY5324913.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(5092..5236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5501..6409
                     /locus_tag="JQR05_002785"
     CDS             5501..6409
                     /locus_tag="JQR05_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAY5324914.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASTMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(6601..8622)
                     /gene="uvrB"
                     /locus_tag="JQR05_002786"
     CDS             complement(6601..8622)
                     /gene="uvrB"
                     /locus_tag="JQR05_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY5324915.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9201..9878)
                     /gene="bioD"
                     /locus_tag="JQR05_002787"
     CDS             complement(9201..9878)
                     /gene="bioD"
                     /locus_tag="JQR05_002787"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY5324916.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTV
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGELPWLAENPENAATGK
                     YINLALL"
     gene            complement(9871..10626)
                     /gene="bioC"
                     /locus_tag="JQR05_002788"
     CDS             complement(9871..10626)
                     /gene="bioC"
                     /locus_tag="JQR05_002788"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY5324917.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(10613..11767)
                     /gene="bioF"
                     /locus_tag="JQR05_002789"
     CDS             complement(10613..11767)
                     /gene="bioF"
                     /locus_tag="JQR05_002789"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY5324918.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(11764..12804)
                     /gene="bioB"
                     /locus_tag="JQR05_002790"
     CDS             complement(11764..12804)
                     /gene="bioB"
                     /locus_tag="JQR05_002790"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY5324919.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            12891..14180
                     /gene="bioA"
                     /locus_tag="JQR05_002791"
     CDS             12891..14180
                     /gene="bioA"
                     /locus_tag="JQR05_002791"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY5324920.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAVGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            14239..14715
                     /locus_tag="JQR05_002792"
     CDS             14239..14715
                     /locus_tag="JQR05_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY5324921.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            15030..15110
                     /locus_tag="JQR05_002793"
                     /pseudo
     CDS             15030..15110
                     /locus_tag="JQR05_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(15120..15206)
                     /locus_tag="JQR05_002794"
                     /pseudo
     CDS             complement(15120..15206)
                     /locus_tag="JQR05_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3606 a         4073 c         3854 g         3729 t
ORIGIN      
        1 ttaccgggca gcactattac gcggatttcc tcgctcagta ttaccaccaa ctggctgggc
       61 tatattttac tggcagggat tatctttacc gcaggcgtgg tggagttgcc ggatcactgg
      121 tatgtcgatc aaactacgct gcgtattctc ggcattggct tactgatgat tatcgcggtt
      181 tatttgtggt tttgcgcttt cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg
      241 gtgctgcctt catggaaatt cgccctcgct cagatgctga tttccagcgt taactggatg
      301 gtaatggggg cgattatctg gctgttactt ggtcaaagcg tgaactattt ctttgtactg
      361 ggcgtgttac tggttagtag tattgctggc gtcatcgtgc atattccggc ggggatcggt
      421 gtgctggaag cggtgtttat cgcactactg gctggggagc atacctcgaa agggagcatt
      481 atcgccgccc tgctcgctta tcgcatcctg tattacttta tcccgctgct gctggcgctg
      541 atctgttacc tgctactgga aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg
      601 atgtgaagtt tagttaagtt ctttagtatc tgcatttacg actaatgaaa aaaacgcgta
      661 tgcctttgcc agacaagcgt tatagttatc gcggcaagta tcccaaagaa gccgataaaa
      721 tagtaataga gtgttaatgc acctgtaaaa gcgcaacgaa ccggactcca caactcttca
      781 ctatggcatt ttcctgccag ttcaaggagc ttatttcgtc catgcgagat aatgcctggc
      841 cataacacaa tcattaagca acttattacc caaatgggcc gttcactcat taatgccgca
      901 ttcacgatga ttaccagggc caaccctgtc aacgtcatca taatgataaa acggtgagaa
      961 ctaggatcaa cattaaataa taaaccggcc agcatactga ttaaaaacat cgcgcccgca
     1021 gtgctgaaca cgatgataat ggcatataca ggaactaaac ttgcaacgac aatcgccacc
     1081 aaagagagta aaaacagata tattacgccc gcgatggcgg catttatctt gttgatatcc
     1141 ttacttaagc catacataca aagtaataat aaccctatgg cgacatacat tggtggtgaa
     1201 tcatatttgg ttgccgtaat ttcgagatac ttagctcccc aggctagtcc tgcggttgcc
     1261 accaaaccta aagcggtaat gagccatata ccgtttaata tcctggcctc gtgctgaaaa
     1321 tctaatttat tgctgttttg cgagtatgat tccattcttt tagttgtcct tagaaaaaat
     1381 attaatattc accatcaata aatttaaaac cacgcataca aaatactttt gtatttatgg
     1441 atacggaaaa cgattccgct cgctcataag gcatatttta tacctggtac ttgttaagtg
     1501 tcgtttttag taagaaaaaa atcccgacac atggccggga tagtgatggt gaaaaagaat
     1561 taacggcggt tgccgaagat ccgtaacaac atcaggaaca ggttgatgaa gtccagatac
     1621 aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg ggtatcaatc
     1681 tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac aaagacaatc
     1741 acgccgatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa gttgaccagc
     1801 gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc acttaaatcg
     1861 cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt aacgacgaaa
     1921 gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt aagacccgtc
     1981 agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc
     2041 gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg
     2101 ttagtgaaca acagctccat cacggccgcg gaattagccg cataccaggc aacaaatgcg
     2161 gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt ttgcaagcca
     2221 gcccggggtt gtacgattaa atcagaacgt gggaatctgt ccatgacgat ctcctgaaga
     2281 tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa
     2341 cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaaa
     2401 ctaccagcgt tttgccgcct gcagatcgct ctcccgggct tcaacccagc ggtcgccttc
     2461 cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca taataaactg
     2521 cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc
     2581 gcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg gccagcggtt
     2641 acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat agtgttcgag
     2701 ggttaatgct ttgacgctgt cgcccaagtt atggttgcgc acctttccgg tgaaggtgac
     2761 taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta cgctgaacgg
     2821 ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc ggcgggaaga
     2881 aagctacttc gtcgccgtca gtcagcggat ggtcaaaact caccagcgtc tggttgacgg
     2941 cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc gcagccaggt
     3001 gctggcgtaa cgcttcaacg gttgggtaat ccgcagccac ttcggttgca tctgctccca
     3061 ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca
     3121 cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat atctttttgc
     3181 accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt taacgcttcc
     3241 atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac ccgattgtgc
     3301 tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg acacagcggg
     3361 atcagatccc aagtgcgttt tgccgcctga ataccggcaa tacgcgcagt ggcaaatacg
     3421 tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg catggtgaca
     3481 aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc caccatgtgc
     3541 gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc aaatgtggat
     3601 ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt tcccatgcgg
     3661 tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta gcgacgcccg
     3721 ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac atacggaaca
     3781 cttcaccaaa accttcaaac tcacggtcga acaacggcag cagcgcttcg ggagcctgat
     3841 caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg tcgctggcga
     3901 tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg gctttatcga
     3961 caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt
     4021 cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata aattcagtgc
     4081 ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac gataagtttt
     4141 gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc cgcagcgccg
     4201 ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt
     4261 taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa acgcgcaggc
     4321 ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc tctccggcgt
     4381 aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc
     4441 agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc ttacggaaga
     4501 gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata gggcgatgct
     4561 ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc agcacggtat
     4621 tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccctgcc atgacctggt
     4681 tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccaaactg tcgacactga
     4741 cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc agacggtaac
     4801 cattggtggt gaccgcaatc tggcggatag cgtcgttttc ccgcacagcg gcgatgatat
     4861 cggtaaagtc gcggcgtaaa gacggctctc ctcctgtcag gcgcactttt tcggtgccca
     4921 gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag cctttattgg
     4981 tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag ttacacacat
     5041 cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag
     5101 ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa
     5161 tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg cagaggctag
     5221 agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat ccacactttc
     5281 gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa taagggaaaa
     5341 tggcggtttt actttgatat acatcatcag ggaggcatag cgtgcatcgt cctttagaga
     5401 tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt
     5461 gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc gtacgctggc
     5521 tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg ttctctcatc
     5581 actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata atggtggctc
     5641 gacggggcgt attcgccgtt cagaaggcgg cattgcttgg ggcgatatgc gcaactgcct
     5701 caaccagctg ataacggaac cgagcgtcgc ctccacgatg tttgaatacc gttttggcgg
     5761 caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgt tggatcacct
     5821 tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag tggatacgca
     5881 tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc aggggcatga
     5941 agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt tattgttaac
     6001 gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg atctcatcat
     6061 tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga aggaaatcgc
     6121 ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg ggcgtgagtt
     6181 gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg agcagtatgt
     6241 tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa
     6301 agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc gtcatgaccg
     6361 ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac ggaaatccga
     6421 aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt
     6481 gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga aaatgtaggc
     6541 ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt cgctatcctg
     6601 ttacgatgcg gcaataaaca actcacgcaa ctgatgcaac tggtcacgaa tttgtgccgc
     6661 ttcttcgaac tccagattct gcgcgtgttg catcatcagc ccttccagtt catggatttt
     6721 ctgctgcaac gctttcggcg acatatccat cggcacatta tccggctcaa caatcgggcg
     6781 cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg ccaggatatc
     6841 gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg
     6901 tttctcacgg cgacgttcgg tttcgccaat cgctttcgcc attgatggag tgatcttatc
     6961 gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa
     7021 cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca ccagcgacac
     7081 ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt cgaactcacc
     7141 cagacgcaag tcgcggataa tctccatacg ctcgacggtg tcgatatctg agtgaagata
     7201 acgcacgcgc tcaccgtgtt cttcgagata ttcggtaaga tcttccgcca ttcgcttggt
     7261 cagtgtggtg accagcacgc gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag
     7321 atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca atccggttgg
     7381 acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat tacccggcgt
     7441 cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact taagcggacg
     7501 gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt tacgcgcccg
     7561 gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat cgacgaccag
     7621 cagcccatcg gcaggcaggt aatcaaacag cgtcggcggt ggctcacccg gtccacgacc
     7681 ggagaggaag cgcgagtagt tttcaatccc cgaacagtag cccagctcgt tcatcatctc
     7741 cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt tgttttccaa
     7801 cagcactttg cgtctggcgg ccagttcttc tttgatctcc tccatcgcct gtacgatgcg
     7861 ctcgcgcggt gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa tagtggaaac
     7921 aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt caaacagttc
     7981 cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tctatcacct cgccacgaac
     8041 gcggaaagta ccacgctgga atgcttgatc attacgagcg tattgcagct ccgccagtcg
     8101 gcgcagaatc gcgcgctgat cgataatcat accgaccgtg agatggagca tcatcttgag
     8161 atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacta caatcacatc
     8221 acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgctcaatat gttcgttaac
     8281 cgaggcatct ttctcaatga aggtgtcgga actcggtaca taggcttccg gctgatagta
     8341 gtcgtagtag gagacgaaat actccaccgc gttttccggg aagaactctt tcatttcgcc
     8401 atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc gctgaaggtc
     8461 agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa gtaacgtctg
     8521 gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctctg gctgatcgcc
     8581 agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct acctgaagga
     8641 gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac aaattatact
     8701 ggataaaaaa acagttcatc accataatat ttctgataca gcgtaaactc cgctgtcaat
     8761 catgagcaaa atttactctg tggcgagata aaactccggc cttaccgggt tatccccaaa
     8821 gcaacggctt ttttaacatt tgtcaagatg agtgatgaca gttttatggc aagagatgcc
     8881 tgttcagtga ctgccattgc atttatctaa ccagttaaaa aataaaagat atttctttga
     8941 gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc
     9001 taactctaat acacatggtt atccacagga atagtggata actgtctcca gcccctatcc
     9061 cccgccgcct gggcaattcc cacattcgcc gcatgaggcg gaagtaaaat ttttttcgtg
     9121 attattttag aatttaattg gttaaattgc agtcaatcga agacgcgatc tcgctcacaa
     9181 tttaaccaaa tacagaatgg ctacaacaag gcaaggttta tgtactttcc ggttgccgca
     9241 ttttctggat tttctgcaag ccaggggagc tctcccagca gcggcgcggg aatcatgcgg
     9301 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc gttcgccacc
     9361 caacccgcca gagtcagtcc ggcgtgttgt attgcctgca cagtcaacat cgcgtgatta
     9421 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
     9481 tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
     9541 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
     9601 ggcctgccct cttgggcgct gatgatgtgc ggcgaggtgg gttctgcgaa ggtgtaagga
     9661 tttaccgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag cgcgtcacta
     9721 ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata acctgccgtc
     9781 cgataaccta ccgccttcgc ggcttgtaaa agtgcacaac tggcgacggt tttccccact
     9841 tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc ccaaaaaaag
     9901 atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca atcgctgcaa
     9961 ctgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag caccgatgcc
    10021 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccat agtgtgatga gctgaatatg
    10081 atgttgataa cgcacgccgt tcagcgactc ttcgatttca tctggcggta aaaagcgatt
    10141 agcatgcgga cgctcgtcca ccgcctgccg cgcctgatgc agttcgggta acgatccctg
    10201 caccagcgtg gtaaacgcga ccacgcctcc ggggcgcacc acccgataca gctcgcggag
    10261 tgccgtggat aaattaccgc accactgcac cgcgagattg ctccatgcaa gatcgaacgt
    10321 cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatgatctg cggcatcttt
    10381 atggcgggct tgcgcaagca ttggcggcga gagatctaac gccgtcacct gcgcgtgacg
    10441 ttcccgccag tggcggctca tccagccagg tccacaaccc gcgtccagta cgtgggtgta
    10501 tttacgctgt ggaagcattg ccagtaaggc gtcagcactc tggcgctgta gatctgcatg
    10561 ttgctcatag tgtgcggctg cccgaccaaa tgccgctgca atggcttgtt tattaaccgt
    10621 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgca gcggttagcg
    10681 ttaagcgcag tcgcgcagta ccagcgggta cggttggcgg gcgaatcgcc gtgacccagc
    10741 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccgacgatca
    10801 atggctggat ggcgctgcat gaatcagcca gcgtaaacgg caaatcctgt actccggcac
    10861 gaaaacgcgc aatgagtgac accagttttt cgcgccgtgc atccccctca tcacggcgaa
    10921 tgaccgccag cgacgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaga
    10981 taaggtggcg ggcgaattgc agcagataat ccgccaccgt acttgagcaa agcaccgcag
    11041 ccccactgac gccaaatcct ttgccaaaag tcactaccag caattctggt tttacctttt
    11101 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcatcatcga
    11161 ccatcaacca gccattgtgc tgttgcgtta cctgctggat ttccgccagt ggcgcgctat
    11221 cgccgtccat gctgaacacg ccttctgtca ccaccagttg ctgccccgga cagggggaag
    11281 caagcagtcg cgccagatga gcgacatcgt tatgagtaaa acggcgaagc tgcgacgggc
    11341 ttaaactggc agcctccagc aatgaggcat ggctaagccg gtcggcaaca atacggtcct
    11401 ctttcgccat catcgcggca atgactgcct gattcgcggc aaaaccagag ataaacagca
    11461 gcgcccgcga atagccaagc cactcggcca gttcttcttc cagcgcctga tgcaccacgc
    11521 tataaccgct gacgtgaccg gagccgccgc taccgatgcc aaattgctcc gccccctgct
    11581 tccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    11641 tcagatactg gcgatcatcc gccaccagcc agcgtccggc tccttgcgcc accggataac
    11701 ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc
    11761 agctcataat gctgccgcgt tgtaatattc gtcggtgtcc ggggtcatca gcgcctgttc
    11821 aagacgttgc tgttgttcgt tatcccctgc cagcacggca gtttgctgcg gatttagccc
    11881 cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg tcagcagttt
    11941 gcaaccgtag aaaatcgagt ttgcgcctgc cataaagcac atcgcctgag tctgttcgtt
    12001 catctgctcg cgtccggcag aaaggcgcac gtaagaggtc ggcatcatga tccgcgctac
    12061 ggcaatggtg cgaataaaat caaaggcatc aacgtcatcg ttatcggcaa gcggcgtgcc
    12121 tttcaccttc accagcatgt tgattggtac gctttccggc ggcgtcggca ggtttgccag
    12181 ttgcagcaat aatccggcgc gatcttttac cgtttcgcct aagcccacaa tgccacctga
    12241 gcagactttg atcccggcat cgcgcacttt ttccagcgta tcgaggcgtt cctgataagt
    12301 gcgtgtggtg atgatattgc cgtaaaactc cggcgaggtg tccaggttgt ggttgtagta
    12361 atccagcccg gcgttcgcga ggcgctgcgc ctgagattca ctcaacgtgc ccagcgtcat
    12421 acacgcctcc agccccatcg cttttacccc ctgcaccatt tgttccaggt acggcatatc
    12481 gcgttcgtgg ggattcttcc acgccgcgcc catacagaag cgcgtcgatc ctgccgcttt
    12541 cgctttgcgc gccgactcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    12601 ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    12661 aatcgacagc aacgtgctga cctgcacctg acgaggatcg aaatgctggc gatgcacctg
    12721 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    12781 tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    12841 ggtgtagact tgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    12901 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctcccctc
    12961 tgccggttta tccggtggcg agcgccgaag gttgcgagct aattttgtct gacggcagac
    13021 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat cacccgcagc
    13081 ttaatgcggc gatgaagacg caaattgatg ccatgtcgca tgtgatgttt ggcggtatca
    13141 cccatgcgcc cgccatcgag ctatgccgca aactggtggc aatgacgccg caaccgctgg
    13201 agtgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg aaaatggcgt
    13261 tgcagtactg gcaagccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    13321 atcatggcga tacctttggc gcgatgtcgg tgtgcgatcc ggacaactca atgcacagcc
    13381 tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccgcaaagc cgcatggatg
    13441 gtgaatggga tgaacgcgat atggttggtt ttgcccgcct gatggcggca catcgtcatg
    13501 aaatcgcagc ggtgatcatt gagccgattg tccagggcgc aggcgggatg cgcatgtacc
    13561 atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg
    13621 ccgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt gaatatgcag
    13681 aaatcgcgcc ggacattttg tgcctcggta aagccttaac cggcggcaca atgacccttt
    13741 ccgccacact taccacgcgc gaggttgcag aaaccatcag taacggcgaa gccggctgct
    13801 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcggcagca aacgccagcc
    13861 tggcgattat cgaatccggc gaatggcagc agcaggtggc ggctattgaa gtgcagctgc
    13921 gcgagcaact ggcaccagcc cgtgatgccg aaatggttgc cgatgtgcgc gtactggggg
    13981 cagtcggtgt ggtcgaaacc actcgtccgg tgaatatggc ggcgctgcaa aaattctttg
    14041 tcgaacaggg ggtatggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    14101 ttattctccc gcaacagttg cagcgtctga ccgcagcggt taaccgcgcg gtacaggatg
    14161 aaacattttt ttgccaataa cgggaagtca gcgtgagggt ttctggctac actttctgca
    14221 aacaagaaag gagggttcat gaaactcatc agtaacgatc tgcgcgatgg cgataagttg
    14281 ccgcatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    14341 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    14401 gcgccaaccg gctccggctg gtggcactgg gtagttgtta atttacccgc tgatacccgc
    14461 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    14521 cgtaccgatt ttggtaaaac cggatacgat ggcgcagcgc cgccgaaagg cgaaacccat
    14581 cgctacattt ttaccgttca cgcgctggat gtagagcgta ttgatgtcga tgaaggagcc
    14641 agcggcgcga tggtcgggtt taacgttcat ttccactctc tggcaagcgc atcgattacc
    14701 gcgatgttta gttaatcact ctgccagatg gcgcaatgcc atctggtatc acttaaaggt
    14761 attaaaaaca actttttgtc tttttacctt cccgtttcgc tcaagttagt ataaaaaagc
    14821 tgaacgagaa acagtaaaaa tcattaatat caatgcatta taatacattt agcttaaaaa
    14881 atagactgca taatactgca aaacacaaca tatccagtca ctatgaatca accacttaga
    14941 tggtattagt gacctgatac agagcattag cgcaaggtga ttttttgtcc tcttgcgcta
    15001 attttttgtc aacgcactgg gttagctgaa tttacaacca tgctcagtat ctcgataagc
    15061 gcagagaaat gatgctgtgg tgggcagact actggcttga tgaaaaggta gggcgaccaa
    15121 ccgcgttttt tcagttcagt cagcacattg agccagaact tcaccccttc attttcggcc
    15181 agccacatcc ccaacagttc ttcctgttct taactattta caggcgcgtt tttttaaaga
    15241 atcctcactc catttgcacg aa
//
LOCUS       DADKXM010000094        15172 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.095, whole genome shotgun sequence.
ACCESSION   DADKXM010000094 DADKXM010000000
VERSION     DADKXM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.095"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..311)
                     /locus_tag="JQR05_002795"
     CDS             complement(<1..311)
                     /locus_tag="JQR05_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HAY5324922.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     G"
     gene            complement(424..2358)
                     /gene="yeaG"
                     /locus_tag="JQR05_002796"
     CDS             complement(424..2358)
                     /gene="yeaG"
                     /locus_tag="JQR05_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY5324923.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYDIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2794..3540
                     /locus_tag="JQR05_002797"
     CDS             2794..3540
                     /locus_tag="JQR05_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY5324924.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3630..4391
                     /locus_tag="JQR05_002798"
     CDS             3630..4391
                     /locus_tag="JQR05_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY5324925.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGVEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(4442..5326)
                     /locus_tag="JQR05_002799"
     CDS             complement(4442..5326)
                     /locus_tag="JQR05_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY5324926.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(5410..6405)
                     /gene="gapA"
                     /locus_tag="JQR05_002800"
     CDS             complement(5410..6405)
                     /gene="gapA"
                     /locus_tag="JQR05_002800"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY5324927.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            6747..7160
                     /gene="msrB"
                     /locus_tag="JQR05_002801"
     CDS             6747..7160
                     /gene="msrB"
                     /locus_tag="JQR05_002801"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY5324928.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            7202..7474
                     /locus_tag="JQR05_002802"
     CDS             7202..7474
                     /locus_tag="JQR05_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY5324929.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            7844..8920
                     /locus_tag="JQR05_002803"
     CDS             7844..8920
                     /locus_tag="JQR05_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5324930.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FMCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            8947..10326
                     /locus_tag="JQR05_002804"
     CDS             8947..10326
                     /locus_tag="JQR05_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324931.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            10343..11386
                     /locus_tag="JQR05_002805"
     CDS             10343..11386
                     /locus_tag="JQR05_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAY5324932.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            11407..12243
                     /locus_tag="JQR05_002806"
     CDS             11407..12243
                     /locus_tag="JQR05_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY5324933.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            12248..13195
                     /locus_tag="JQR05_002807"
     CDS             12248..13195
                     /locus_tag="JQR05_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY5324934.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            13205..14185
                     /gene="ydjG"
                     /locus_tag="JQR05_002808"
     CDS             13205..14185
                     /gene="ydjG"
                     /locus_tag="JQR05_002808"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY5324935.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKLIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            14322..15080
                     /locus_tag="JQR05_002809"
     CDS             14322..15080
                     /locus_tag="JQR05_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5324936.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
BASE COUNT         3935 a         3405 c         3856 g         3976 t
ORIGIN      
        1 tggccctgac cactgccgga acctccgccg ccaccctggg gacgttcaat tcggtcgttc
       61 tgaacgaaat ggtcattgcc cggatgcacg cggtggcgca gaccgccacg cccctgatga
      121 aacattggtt cgctaatatc ttccgtggga atggatacgg attcgccgct gtcgacgtca
      181 gtcaccgaac gcttattaat ggcctcggag atcgactgtt taatttgcgc tttataacgg
      241 cgtaaaaaac gctggcgatt caccatgctt ttgtttttgc cgttcagacg ccggtcaata
      301 aaccaggtca tatgcccccc gtactacatt tgccaactgt atgctacgcc tgtgttgccg
      361 cttctcaggc ccacggttcg ctgtcgttgt aggcccggta gcatgtgtac cgggccaggg
      421 ttgttaagac gatttacgta cgcgcaaata ccattcgcac agtaaacgca cctgtttacg
      481 ggtgtagcct ttttccatca tacggtcgac aaagtcgtcg tgtttcttct gctcgtcggt
      541 tgacgttttg gcgttaaacg agataaccgg caacagctcc tcggtattgg agaacatttt
      601 cttctcgata accgtgcgca gtttttcata gctggtccag ttcggattgc gtccgctgtt
      661 attcgctctg gcgcgcagta cgaagttaac aatctcgttg cggaaatctt ttggattact
      721 gatccccgcc ggtttctcga ttttctccag ctcggcgtta agagactcgc ggtcaaacag
      781 ctgcccggta tccggatcgc gatactcctg atcctgaatc cagaaatccg cgtaggtaac
      841 ataacggtcg aaaatgtttt gcccatattc ggaataggat tcaaggtagg ccgtctggat
      901 ctctttgccg ataaactcgg catatttcgg gatcagataa cctttcagga actccagata
      961 gcgttctgcc tgctcttgcg ggaactgctc gcgctcgatc tgctgttcca ggacgtagaa
     1021 cagatggacc gggtttgccg ccacttctac atgatcgaag ttgaacacgc gggagaggat
     1081 cttaaacgca aaacgcgtcg acagaccgtt catcccttca tcgacaccgg cgtagtcacg
     1141 atattcctga tacgacttgg ctttcggatc ggtgtctttc agactttcgc catcataaac
     1201 ccgcatcttt gaataaatgc tggagttttc tggctctttc aggcgcgaaa gaatggaaaa
     1261 acgtgacagt gtttcgagcg tgccaggggc gcatggggcg tgagtcaatt cactgtgatt
     1321 aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc aagcaatacg gcaccttcac
     1381 gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta ttacggaaag tgacccattc
     1441 ggactcgttc gagtgggcga gaataatccc gttgaacggc agggcggaga taccttccgt
     1501 cccgttgtag ttaccttcct gagtggcggt taacaaggga tgcagcactt ttatcggtgc
     1561 tttaaacatc tcaacgaatt ccatgatccc ctgattggcg cggcacagcg caccggaata
     1621 gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt ttacgaatat cgactttccc
     1681 aaccagcgcg gagatgtcct ggttgttctc atcaccgggt tccgttttgg cgatagcaat
     1741 ttgttgcaga attgacggcc agaccttcac tacccggaac ttagtgatat cgccaccaaa
     1801 ttcatgcagg cgttttgccg cccatggcga catgatggtg ccgagataac ggcgaggaat
     1861 gtcatactct ttttccagaa tctgagcatc ttcctgcgga ttgaaaagac agaacggatg
     1921 atcgttgacc gggctacgct caccgttggc gctcaataca taaatcggta cgagctgcat
     1981 taatgatttc agtcgctcag caagcgatga tttaccccca cccacaggcc ccagcagata
     2041 caggatttgt ttcttctctt ccagcccctg agccgcgtgt ttcagataag agacaatctg
     2101 ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc ggataacgtg caatgacccg
     2161 gttagaaaag agtcgagaaa gtctgggttc ctgggctgta tcgaccatga caggctcacc
     2221 gatagccatc aatagccgct cagccgcgtt ggcataagca ctgcgatctt gccgacaagt
     2281 ggtaagaaac tcctgcagtg tgaactcttc gtccttggca gcttcatatc gctggcgata
     2341 gtgatcgaat atattcatgg tatgccgtcc tttcgttttt tagcacaggt taagagccgt
     2401 tcatatgaat agtagaggct cccggaagag gtaaactgaa cgacgtctct tgctaaaaca
     2461 cagcaactct catgccattt tatcgacgat gagaacaacg tgcccggtga tacaccttca
     2521 aaattaagcg tagatggcaa atggaaaact tgcctgcacg gctttcttaa tttcaatggc
     2581 atatcaacaa ctcatccata aattttgcat aattaatgta aagaccaggc tcgccagtaa
     2641 cgctaaattc atttggctgt aagcgcggtg tcatccgcgg caggaaaatt aaacagttac
     2701 tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg
     2761 cccgctaaac atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt
     2821 ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc
     2881 gcaggcgtag gtgtcgttga gcacccatat aaagattacg ataccgatgt ttacccagta
     2941 ccggtaatca actatgaagg cgataacttc tggttccgtg gcttaggtgg tggttactac
     3001 ctgtggaatg acgcaacgga taaactttca attaccgctt actggtcgcc gctttacttc
     3061 aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg
     3121 atggctggtc tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct
     3181 ggcgataccc tggataacag caacggcatt gtctgggata tggcctggtt gtatcgttac
     3241 accaacggtg gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag
     3301 aacgaatact attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat
     3361 aacccgaatg acagctggag cccttacctt gagctgagcg ccagctacaa cttcctcggc
     3421 gactggagtg tttacggtac cgcgcgctac acccgtctgt ctgatgaagt tactgacagc
     3481 ccgatggtgg ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga
     3541 taatgcatct ttccagggca ttttgtgcat aaaaacaggg cgtcacgccc tgttttgcat
     3601 tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg
     3661 atgtctcact gccagccata gggcagggaa catggtatat gggcgaagat gccagtcagc
     3721 gcaaaacaga agttgctgca ctacgcgcag gcattgaact cggtttaacc ctcattgata
     3781 ccgccgaaat gtatgccgat ggcggtgtcg aaaaggtggt tggggaagca ttaaccggtc
     3841 tgcgagagaa ggtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag
     3901 cgataaatgc atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact
     3961 tattacactg gtctggcagt ttcgcttttg aagagactgt tgctgcgatg gaaaaattga
     4021 tcgcccaggg aaaaatccgt cgctggggcg tttctaacct tggttatgcc gatatgcagg
     4081 aactctggca gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg
     4141 gttcacgagg gattgagtac gatctactcc cctggtgcca gcaacagcag atgccggtga
     4201 tggcttacag tccattagcc caggccgggc ggttgcgcaa tggactgtta aaaaacgcgg
     4261 tagtcaacga aattgcacat gtccaacaaa atgcagctgc gcttgaggtc gaactttcgt
     4321 cagcggaatt agctatgctg gataaggcat atccggcacc aaaaggaaaa actgcgctgg
     4381 atatggtgtg atgttgatac gtgccggatg catcgcaccc ggcacacaac attaacgcaa
     4441 attaacgttt ggcaacgcga atggattgcg ccagatgtgc aggtttctct ttggtgactt
     4501 tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa ggtcttgtag ccatcgtccg
     4561 gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt ccagccgaca acgttcagat
     4621 gatgctggtg gcctacggcg ataatacgat tcagcgcttc atcattaatc acgctgcaat
     4681 cttgcggatt cagatacacg cgatcggtac ggtcagggaa ggtctgaata ccgtcggtca
     4741 gtacatcttc tttcgcgtca ttcactttat caatgaagcg atcgcccagc ccactgacgc
     4801 ttaccttagc gatatcaccc acgttaaagt aggtatgcag ggcagaggtg gtttcaaatt
     4861 cgccatgtga ttcaagatcg atttcacagg ttttacccac gcggaaatgc gctaacagcg
     4921 taaagtcgtg cggccagaat tttttcgtct cttcgctttg tgtcaattca aaagtcagcg
     4981 ctacgccatc agcatcttca tgatgtgatt tcagcgtcca cggcaggttg cgggcaaaac
     5041 cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca cggccagcaa accggtacgc
     5101 caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt gctcaaccac agaacttctt
     5161 cttcacccgc aggtttccac gagagaaggt gtgcgccctg taatgcaaaa gaggctttta
     5221 cctggggatg atcgaccaca atgaggtcca gttcatccag tttacgacgg gagaggacag
     5281 gggagatttg ttcgatgacc ggaagggcaa aaattttctt aatcatgatg cagtccttta
     5341 acttcatttt atcaggtaaa aaaaagagcg accgaagtcg ctctttttag atcacagtgt
     5401 catctcaact tatttggaga tgtgagcgat caggtccaga actttgttgg agtaaccggt
     5461 ttcgttgtcg taccaggata ccagtttcac gaagttgtcg ttcagagcga taccagcttt
     5521 agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg gtagatacta cgtcatcttc
     5581 ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca gctttaacgg cagctttgat
     5641 ctgctcgtaa gttgcagctt tttccagacg aacggtcagg tcaactacgg atacgttcgg
     5701 ggtcggaacg cggaacgcca taccagtcag tttgccattc agttctggca gtactttacc
     5761 tacagcttta gcagcaccgg tagaggacgg gatgatgttc tgggaagcgc cgcggccgcc
     5821 gcgccagtct ttgtgagacg ggccatcaac ggttttctga gtagcggtag tagcgtgaac
     5881 ggtggtcatc agaccttcga tgatgccgaa gttatcgttg ataactttag ccagcggagc
     5941 caggcagttg gtggtgcagg aagcgttgga aacgatgtcc tggccagcat atttgtcgaa
     6001 gttagcgcct ttaacgaaca tcggagtgtt gtctttagac ggaccagtca taaccacttt
     6061 cttcgcacca gcggtgatgt gtttacgagc agtttcgtca gtcaggaaca gaccagttgc
     6121 ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg ttagccggat cacgttcagc
     6181 ggtaacacgg atttttttac cgttaacgat cagatgaccg tctttcactt caacggtacc
     6241 gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat gccatgtaat cagcgtctaa
     6301 caggtcgttg attgcaacga tctcgatgtc agaacgtttc tgagcagcac ggaaaacaat
     6361 gcgaccgata cggccaaaac cgttgatacc tactttgata gtcatatatt ccaccagcta
     6421 tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa ccttacgcag cgtcaagcgg
     6481 aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa gcattacgcg actgactcgc
     6541 ctcactcttc ctttgggctt gagaccacat ggggtcggcg ccccgaattt taaagggcaa
     6601 ttacgataaa aatgtgattt tcatcacgat ttaggtgcag ccatttcgtc gagatcaagt
     6661 ttaggacaaa attttgccag ttggtgttaa tgttttgtta gaatcagtca cgtaatgtga
     6721 gcacgattaa agtgagatgt gagtaaatgg ctaataaacc ttcggcagaa gaactgaaaa
     6781 aaaatttgtc cgagatgcag ttttacgtga cgcagaatca tgggacagaa ccgccattta
     6841 cgggtcgttt actgcataac aagcgtgacg gcgtatatca ctgtttgatc tgcgatgccc
     6901 cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg ctggcccagt ttctacgaac
     6961 cggtgagtga agaatccatt cgttatatca aagacttgtc acatggaatg cagcgcatag
     7021 aaattcgttg cggtaactgt gatgcccatc tggggcatgt ctttcccgac gggccgcaac
     7081 caacaggcga acgttattgt gttaactctg cttctttacg cttcactgat ggcgaaaacg
     7141 gcgaagaaat caacggttga aaaaacgatt cagcaactta ttccacagga gcggaaaaat
     7201 tatgaatctt gatgacatta tcaacagcat gacgcctgag gtataccagc gtttgtcgac
     7261 cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg accgaggaac aaaaagagaa
     7321 ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat accgaagcac agcatatgac
     7381 aattgacaca aatggtcaga tggtcatgaa gagcaaacag cagttaaaag aagattttgg
     7441 tatctcagca aagcctattg ccatgtttaa gtaagtggtt catccccttt ttgccgcggc
     7501 tatgaagggg atagccaaca atgaccccac gactcgtctc aattatctcg acccgacctg
     7561 gctaagtccc agtccgatct cttgcagtcg cgttcacgcc tgaatgactt ctcctgaact
     7621 taagtctcag acctatttgg ccggtaatcc ctctcgaatt tgctcttttt gcgtcccgcc
     7681 ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa ttgtgatcta agttgtaaaa
     7741 cgcaggtcat tatctgtgtt atgcagattt aatgttgacc tcaaaaatca tgtgggtaag
     7801 tatgatggcc atctaagcaa aacaaaacac agaggatgac gcaatgaaag cactggctcg
     7861 gtttggcaag gcctttggcg gctacaagat gattcatgtc ccacaaccca tttgtggccc
     7921 ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc ggcgcagaca tgaagcacta
     7981 caatgtcgat agcggttctg atgagtttaa ctctatccgc ggccatgagt tcgcaggttg
     8041 tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg gggcaacgcg tcgtgtcgga
     8101 taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa cagggtgatt ttatgtgttg
     8161 tacagaaaaa gtaaaccttg gtctggataa caatacttgg ggcggtggtt tttccaaata
     8221 ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat gcgttgtggg aaatccctga
     8281 tggtgttgat tatgaggacg cagccgtact tgaccctatc tgtaatgcct ataaatccat
     8341 cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc gtcattggca ctggcccact
     8401 cgggctgttt tccgtacaaa tggcgcggat tatgggggcg gtaaatatcg tcgtcgttgg
     8461 tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa gaactggggg cgacggcagt
     8521 agtaaatggt tctaccgaag atgtggtggc gcgctgccag caaatttgtg gcaaagataa
     8581 tctgggactg gtgattgaat gctccggtgc caatatcgca ctgaaacaag ccatcgaaat
     8641 gctccgcccg aatggggaag tggtacgcgt tggaatgggc ttcaaacctc ttgatttctc
     8701 gattaatgac attaccgcct ggaataaaag catcattggg catatggcct atgactccac
     8761 ctcatggcgt aacgctatcc ggctattagc cagcggcgct atcaaagtca aaccgatgat
     8821 cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt gatgcgatgg tcgataaaac
     8881 cgcaatcaaa gtgatcatga cttacgactt tgatgaataa ctactctcct gaaggaacgt
     8941 tatcgcatgg aacagataac aaaaccgcat tgtggtgcac ggctggatcg cttaccggat
     9001 tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg gcttgcttgt ctgctggagt
     9061 aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt tgggctggac agataattcc
     9121 accacagcca cattctcagc aatcacgacc gccggaatgt ttctcggtgc gctggtgggc
     9181 ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca tcctctatga ggccattcat
     9241 attgcctcga tggttgtcgg tgctttctca ccgaatatgg attttcttat tgcctgccgt
     9301 tttgtgatgg gcgttgggct gggagcttta ctggttacac tgtttgctgg tttcaccgaa
     9361 tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg tttcctttat tggcaactgg
     9421 tcatatccgc tctgctcact gattgcgatg ggactcacgc cgctgattag tgcagagtgg
     9481 aactggcggg tacaactgct tattcctgca atattgtcgc ttatcgctac ggcgctggcc
     9541 tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc gcggacggta tcaggaagcc
     9601 gagaaagtca tgcggagtat agaagaaggc gtcatacgcc agacgggtaa acctttgccg
     9661 cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg tgccgtattc agccttactg
     9721 acaggagtat tactgaaacg cgtgatatta ggctcttgtg tgctgattac catgaacgtt
     9781 gtgcagtaca cactaattaa ctggttgcca acaatattca tgacccaggg gattaattta
     9841 aaagactcga ttgttttaaa taccatgagt atgtttggcg cgccatttgg tatttttatt
     9901 gccatgctgg tgatggataa aattccgcgt aaaacaatgg gtgtggggct attaatcctg
     9961 attgcggtgc tcggatatat ctattcactg caaaccagta tgttgctcat aacgctgatt
    10021 ggtttcttcc tgattacttt cgtctatatg tacgtttgct atgcctcggc agtgtatgtc
    10081 cctgaaatct ggccgacaga ggccaaactc cgtggctccg gtctggcgaa tgcggtaggg
    10141 cgaatcagtg gtattgccgc accttatgcc gttgcagtgc tgctcagtag ttatggtgta
    10201 acgggagtct ttattcttct gggggctgtt tcaattattg tcgcaattgc tatcgccacc
    10261 attggaattg aaaccaaagg tgtctccgtt gaaagtttaa gtattgatgc agtagccaat
    10321 aaataatacg ggtggattca aaatgaaaaa ttcaaaagca atattgcagg tgccgggcac
    10381 aatgaaaatt atttcagcag aaataccagt gcctaaagaa gatgaagttt tgattaaagt
    10441 agaatatgtc ggtatttgtg gttcagatgt acatggtttt gaatcaggcc cgtttattcc
    10501 gcctaaagac ccaaatcaag aaattggcct gggtcatgaa tgcgccggga cggttgtggc
    10561 tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg gtaaatatcg aacctggcgt
    10621 tccttgcggt cactgtcgtt actgtctgga aggcaaatat aacatctgcc cggacgttga
    10681 ttttatggcg acacaaccca actaccgcgg cgcattaacg cactatctgt gtcatccgga
    10741 gagctttact tacaaactgc ccgacaatat ggacacgatg gaaggggcgc tggtggagcc
    10801 tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt aaaccgggta agaagataat
    10861 tattctggga gcaggttgta ttggtttgat gacgttgcaa gcgtgcaaat gcctgggcgc
    10921 aacggaaatt gccgtcgttg atgtactgga aaaacgtctg gcaatggcgg aacagcttgg
    10981 tgcgacagtg gttattaatg gcgcaaaaga ggacactatt gcacgctgtc agcaatttac
    11041 cgaagacatg ggcgcagata ttgttttcga aacagcgggt tctgcggtca ccgttaaaca
    11101 ggcaccttat ctggtaatgc gcggcggtaa aattatgatt gttggtactg tacccggcga
    11161 ttcggcaatc aatttcctca aaatcaatcg cgaagtcact atccagacgg tattccgcta
    11221 tgccaatcgt tatccggtca cgattgaagc tatttcttca gggcgattcg atgtgaaatc
    11281 gatggtgacg catatttacg attatcggga tgtacaacag gcatttgaag agtcagttaa
    11341 caacaaacgc gacattatta aaggcgttat taaaattagc gattaattaa tacgggagaa
    11401 cagaacatgc tcgcagatat cagatattgg gaaaacgatg ccacaaataa acattatgca
    11461 attgcccatt ttaacgtatg gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa
    11521 gaagcgaaat ccccggttat tatttctttt ggtacaggtt ttgtcggtaa cacctcattt
    11581 gaagatttct ctcacatgat ggtatcaatg gcacaaaaag caacggtgcc ggtaataact
    11641 cattgggatc atggtcggag tatggagatt attcataacg cctggactca tggtatgaat
    11701 tcattaatgc gtgatgcttc tgcatttgat ttcgaagaaa atattcgttt aaccaaagag
    11761 gctgttgatt tctttcatcc gctgggtatt ccggtagagg cggaattagg gcatgtcggt
    11821 aatgaaaccg tttatgagga agcgctggcg ggctatcact ataccgatcc tgaccaggcg
    11881 gctgaatttg ttgaacgtac gggctgtgac tcattggctg tcgccatcgg caaccagcat
    11941 ggggtttata cgtcagagcc gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct
    12001 gtttctgttc cgctggtttt gcatggcgca tcggggatca gtgatgccga cattaaaact
    12061 gcaatttcgt tgggtattgc gaaaatcaac attcatacgg agctctgtca ggccgcaatg
    12121 gtagcagtga aagaaaacca ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa
    12181 gctgtaaaag aacgggcatt ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa
    12241 taatgatatg gataatctcg acgttatttg tataggtgcc gctattgttg atattccatt
    12301 acaaccggtc agtaaaaata tctttgatgt agattcttat cctcttgaaa gaatcgcaat
    12361 gagcaccggg ggcgatgcaa taaatgaagc aacaattatt tctcgtctgg ggcatcgtac
    12421 cgcattaatg agccgcattg gtaaagatgc ctcagggcaa tttattctcg accattgtcg
    12481 caaagagaat attgatattc aaagcctgaa acaggacgtc agcatagata catctattaa
    12541 cgttggactg gtgacggagg atggcgagcg gacgtttgtc acgaaccgta acggcagttt
    12601 gtggaaatta aatattgacg atgttgattt tgcgcggttt cctcaggcga aattattatc
    12661 actggccagt attttcaaca gtcctctatt ggatggtaaa gcgctaacag aaatttttac
    12721 gcaagcgaaa gcccggcaga tgatcatttg tgccgatatg atcaaaccgc ggttgaatga
    12781 aacgctggat gatatttgcg aagcattgag ttatgtcgat tatctgtttc caaattttgc
    12841 cgaggcaaaa ttactcacag ggaaagagac actggatgaa attgctgact gctttcttgc
    12901 gtgcggcgta aaaacggtgg tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg
    12961 tgacatgacg atgaaggtgc cggcggtcgc aggaataacc gccatcgaca ccattggcgc
    13021 gggcgataac tttgcttcag gttttattgc ggcactgtta gaaggcaaaa atctgcgtga
    13081 atgcgcacgc tttgccaatg caacggcggc tatctcggtt ctaagcgtcg gtgccaccac
    13141 cggcgtaaaa aacagaaagc tggtcgaaca attgctggaa gaatacgaag gataatgaag
    13201 gcaaatgaaa aagatacctt taggcacaac ggatataacg ctttcgcgaa tggggttggg
    13261 gacatgggcc attggcggcg gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat
    13321 tgatacgatt cttgaagccc atcgttgtgg cattaatctg attgatactg cgccaggata
    13381 taactttggc aatagtgaag ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca
    13441 ggttgtagta gaaaccaaat gcggcattgt ctgggaacga aaaggaagtt tattcaacaa
    13501 agttggcgat cggcagttgt ataaaaacct ttccccggaa tctatccgcg aagaggtaga
    13561 agccagcttg caacgtctgg gtattgatta catcgatatc tatatgacgc actggcagtc
    13621 ggtgccgcca ttttttacgc cgatagctga aactgtcgca gtgcttaatg agttaaaggc
    13681 tgaagggaaa attcgcgcta taggcgctgc taacgtcgat gctgaccata tccgcgagta
    13741 tctgcaatat ggtgaactgg atattattca ggcgaaatac agtattctcg accgggcaat
    13801 ggaaaacgaa ctgctgccgc tatgtcgtga taatggcatt gtggttcagg tttattcccc
    13861 gctagagcag ggattgttga ccggcaccat cactcgtgat tacgttccgg gcggcgctcg
    13921 ggcaaataaa gtctggttcc agcgtgaaaa catgctgaaa ttgattgata tgcttgaaca
    13981 gtggcagcca ctttgtgctc gttatcagtg cacaattccc actctggcac tggcgtggat
    14041 attaaaacag agtgatttaa tctccattct tagtggggct actgcaccgg aacaggtacg
    14101 cgaaaatgtc gcggcactga atatcaactt atcggatgca gacgcaacat tgatgaggga
    14161 aatggcagag gccctggagc gttaaatact caacacgatt cacctctgtt tctgtgaaaa
    14221 ctgtgatgaa aatctgtgat tcaaataggt tattttgaaa gtaaacatcg gttatgcgat
    14281 aatcgcgcta atgtgatgta aaaagataca ggggtgtaat cgtggcggca aaagacagga
    14341 ttcaggcaat taagcaaatg gttgccaacg ataaaaaggt gacagtctca aatttgagtg
    14401 ggatttttca ggtaaccgaa gaaaccattc gccgcgatct tgagaagctg gaagatgaag
    14461 gctttttgac cagaacctat ggtggtgctg ttttaaatac agcgatgttg acggagaata
    14521 tccattttta taagcgcgct tcatcgtttt atgaagagaa gcagcttatt gcacgcaagg
    14581 cactaccctt tatcgacaat aaaaccacca tggcagccga ttccagtagt accgtaatgg
    14641 aattgctcaa attgttacag gaccgtagtg acctgacgtt gctaaccaac tcggcggaag
    14701 cgattcatgt tctggctcag tcagaaatta aagtcgtttc aacgggtggg gaactaaaca
    14761 aaaatacact ttccctgcaa ggaagaatta ctaaagagat catcagccgc taccatgtcg
    14821 atatcatggt aatgagttgt aaaggtcttg atattaacag cggcgcgctg gactctaatg
    14881 aagcggaagc tgaaatcaaa aagacaatga tccgtcaggc gacagaagtt gcgttattgg
    14941 ttgatcactc taagtttgat cgtaaagctt ttgtccagtt agctgacttt agtcatatta
    15001 attatataat aactgataaa tcaccgggtg cagagtggat agcattttgc aaagacaata
    15061 atatccagct ggtgtggtaa tttaagcaca cgttgtcaat attatttgtt cctgcgcctg
    15121 gaaataaaat gatgataaag atgcggcaag tgaatttgcc gcaattgcta cc
//
LOCUS       DADKXM010000095        15063 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.096, whole genome shotgun sequence.
ACCESSION   DADKXM010000095 DADKXM010000000
VERSION     DADKXM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.096"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..766
                     /gene="citC"
                     /locus_tag="JQR05_002810"
     CDS             <1..766
                     /gene="citC"
                     /locus_tag="JQR05_002810"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY5324937.1"
                     /translation="TEYEALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRH
                     PGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGT
                     ADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRF
                     VGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKN
                     DLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
     gene            781..1077
                     /gene="citD"
                     /locus_tag="JQR05_002811"
     CDS             781..1077
                     /gene="citD"
                     /locus_tag="JQR05_002811"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY5324938.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1074..1982
                     /gene="citE"
                     /locus_tag="JQR05_002812"
     CDS             1074..1982
                     /gene="citE"
                     /locus_tag="JQR05_002812"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY5324939.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            1993..3524
                     /gene="citF"
                     /locus_tag="JQR05_002813"
                     /pseudo
     CDS             1993..3524
                     /gene="citF"
                     /locus_tag="JQR05_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            3528..4079
                     /gene="citX"
                     /locus_tag="JQR05_002814"
     CDS             3528..4079
                     /gene="citX"
                     /locus_tag="JQR05_002814"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY5324940.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4054..4932
                     /gene="citG"
                     /locus_tag="JQR05_002815"
     CDS             4054..4932
                     /gene="citG"
                     /locus_tag="JQR05_002815"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY5324941.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTIVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            4983..6446
                     /gene="citT"
                     /locus_tag="JQR05_002816"
     CDS             4983..6446
                     /gene="citT"
                     /locus_tag="JQR05_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY5324942.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6560..7366
                     /gene="rna"
                     /locus_tag="JQR05_002817"
     CDS             6560..7366
                     /gene="rna"
                     /locus_tag="JQR05_002817"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY5324943.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7595..8005
                     /gene="rnk"
                     /locus_tag="JQR05_002818"
     CDS             7595..8005
                     /gene="rnk"
                     /locus_tag="JQR05_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY5324944.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8236..9474)
                     /locus_tag="JQR05_002819"
     CDS             complement(8236..9474)
                     /locus_tag="JQR05_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY5324945.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            9695..10123
                     /gene="uspG"
                     /locus_tag="JQR05_002820"
     CDS             9695..10123
                     /gene="uspG"
                     /locus_tag="JQR05_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY5324946.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10244..11809)
                     /gene="ahpF"
                     /locus_tag="JQR05_002821"
     CDS             complement(10244..11809)
                     /gene="ahpF"
                     /locus_tag="JQR05_002821"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY5324947.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12054..12617)
                     /gene="ahpC"
                     /locus_tag="JQR05_002822"
     CDS             complement(12054..12617)
                     /gene="ahpC"
                     /locus_tag="JQR05_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY5324948.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12989..13735
                     /gene="dsbG"
                     /locus_tag="JQR05_002823"
     CDS             12989..13735
                     /gene="dsbG"
                     /locus_tag="JQR05_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY5324949.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            13945..14846
                     /locus_tag="JQR05_002824"
                     /pseudo
     CDS             13945..14846
                     /locus_tag="JQR05_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14993..15055
                     /locus_tag="JQR05_002825"
                     /pseudo
     CDS             14993..15055
                     /locus_tag="JQR05_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate
                     sulfotransferase"
BASE COUNT         3657 a         3940 c         3707 g         3759 t
ORIGIN      
        1 aaccgaatac gaggcgctgt tccgccagtg cggtttttcc acgctgacct gcgtacccgg
       61 cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa cgctatgccg aatcgctgaa
      121 aaaatttcgt catccaggga acaagattgg ctgcattgtg atgaacgcca atccctttac
      181 gaatggtcac cgttatctga ttcaacaagc tgcggcacag tgcgactggt tgcatctgtt
      241 tttagtcaaa gaagattctt cacgcttccc ctatgaagac cggctggatc tggtgttaaa
      301 aggcaccgcc gatattccac gcctgactgt gcatcgcggc tcagaataca tcatctcccg
      361 cgctacgttc ccttgctact tcattaaaga acagagcgtc attaaccatt gttacaccga
      421 aattgatctg aagattttcc gtcagtacct cgctcccgcg cttggtgtaa ctcaccgctt
      481 tgtcggtact gaaccctttt gtcgcgttac cgcccagtac aaccaggata tgcgctactg
      541 gctggaaacg ccgactatct ccgcagcgcc catcgaactg gttgaaattg agcggctgcg
      601 ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct
      661 cacggctatc gcgccgctgg tccctgcagt cacgctgcat tatttgcaga acctgcttga
      721 gcactcccgc caggacgcgg cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa
      781 atgaaaataa accagcccgc cgttgcaggc acccttgagt ctggggatgt gatgatacgc
      841 atcgccccac tcgatacgca ggatatcgac ctgcaaatca atagcagcgt tgagaaacag
      901 tttggcgatg caattcgcac caccattctg gacgttctcg cccgctacaa cgtgcgcggc
      961 gtacagctga atgtcgatga caaaggcgca ctggactgca ttttacgtgc acgactggaa
     1021 gccctgctgg cacgcgccag cggtatcccg gctctgccat gggaggattg ccaatgattt
     1081 ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag catgttgttt gtgcctggtg
     1141 ccaatgccgc gatggtcagc aactccttca tctacccggc tgatgccctg atgtttgacc
     1201 tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg ccgcatggtt tatcacgcgc
     1261 tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg tgtcaacgcg ctggattccg
     1321 aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc
     1381 cgaaaaccga taccgctcag gatgttctgg atattgaaaa agagatcctg cgtatagaaa
     1441 aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc ggcgattgaa tctccgctgg
     1501 gcattacccg cgcagtggaa atcgctcacg cttccgagcg tttgatcggt atcgccctcg
     1561 gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc cccggaagga actgaactgc
     1621 tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc gggtattcag gcgttcgata
     1681 ccgtctattc cgacgctaac aacgaagccg gattcctgca agaagccgcc cacatcaaac
     1741 agctgggctt tgacggcaaa tcgctgatca acccgcgtca gattgatctg ctgcacaacc
     1801 tctacgcacc gacccagaaa gaagtggatc acgcccgccg cgtcgtagaa gccgctgaag
     1861 ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg gacggtccgg
     1921 ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact ttccggcatc cgcgaagaat
     1981 aaggcaatca aaatgacgca gaaaattgaa caatctcaac gacaagaacg ggtagcggcc
     2041 tggaatcgtc gcactgaatg cgatcttgcc gctttccaga actcaccaaa gcaaacctac
     2101 caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc
     2161 tcaggtttac aggacggcat gacggtttcc ttccatcacg ctttccgtgg cggtgacctg
     2221 accgtcaata tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct gaccctggcg
     2281 tccagctccc tgagtgattg ccatgcgccg ctggtagaac acattcgcca gggcgtggtt
     2341 acccgcattt atacctctgg cctacgcggt ccactggcgg aagagatctc ccgtggtctg
     2401 ctggcggaac cggtgcagat ccactctcac ggcggtcgtg tgcatctggt acagagcggc
     2461 gaactgaata tcgacgtggc tttcctcggc gtcccgtcct gtgatgaatt cggtaatgcc
     2521 aacggctaca ccggtaaagc ctgctgcggc tccctcggct atgcaatggt tgatgccgac
     2581 aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc cttatccgca taatccggca
     2641 agcattgagc aagatcaggt tgatttgatc gtcaaagttg atcgcgttgg cgatgctgca
     2701 aaaatcggcg ctggtactac ccgtatgacc actaacccgc gcgaactgct tattgcccgt
     2761 agcgctgcgg atgtgattgt caactctggc tacttcaaag aaggtttctc catgcaaacc
     2821 ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc
     2881 gatattcgcg ccgacttcgc cctcggcggt attaccgcga cgatggttga tctgcacgaa
     2941 aaaggtctga tccgcaaact gctggatgtg cagagctttg acagccatgc tgcgcaatcg
     3001 ctggcccgta accccaatca catcgaaatc agcgccaacc agtacgctaa ctggggtcga
     3061 aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaggc
     3121 agttcaacgt taacgtgctg accggttctg acggcgtact gcgtggcgct tccggtggtc
     3181 actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccactg gtacgcggtc
     3241 gtattccgac tctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     3301 ttctggtcac agaccacggt atcgcagtta acccggcacg tccggaacta gcagaacgtc
     3361 tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc
     3421 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     3481 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     3541 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     3601 ggcaagcacg gcaacacgcc tggctcaagc accatcctgt tccactggtc tcctttaccg
     3661 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     3721 tgacagcctt gcgtgcctta gccacaaagc agggctggca aattcaggag caggctgcac
     3781 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
     3841 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     3901 tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
     3961 gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg
     4021 atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa
     4081 accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     4141 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     4201 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     4261 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactccac
     4321 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     4381 aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg
     4441 cttcagctca accagccggt tacgccaaca atcgtttgtt ctacggcggc aagtttctgc
     4501 cgtggcctga ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg
     4561 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
     4621 gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     4681 ctggcattgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     4741 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     4801 aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     4861 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctggttttta
     4921 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     4981 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggtg
     5041 tcatgtttct tatccctgtc cccgacggta tgccgccgca ggcatggcat tacttcgctg
     5101 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt
     5161 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     5221 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggcctg gctggttttt
     5281 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
     5341 ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catgggcaaa cgcacgctga
     5401 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
     5461 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
     5521 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
     5581 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
     5641 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
     5701 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
     5761 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
     5821 tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
     5881 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg gcagtttcgc
     5941 taatgctggc tctgcacgtt gtgccgtgga aagacattac ccgctataac agcgcatgga
     6001 acacgctggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggtt
     6061 ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca ccaaacgcaa
     6121 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
     6181 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
     6241 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
     6301 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
     6361 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct
     6421 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
     6481 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg
     6541 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
     6601 tctgcttccc ttatcttctg ccaacgccgt agcgttgcag gcaaaacagt atggcgattt
     6661 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
     6721 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
     6781 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
     6841 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
     6901 acgcgccagc cgaatgtgtt catcgccgga aaccgggtta tcactggaaa cggccgctaa
     6961 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
     7021 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
     7081 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
     7141 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
     7201 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
     7261 caaagctgac gccatcaacg ccccgctttc tgcaaactca ttcttgccac aacctcaccc
     7321 aggtaactgt ggcaaaacct ttgtgattga taaagcgggt tattaatcct ctgatttaca
     7381 aaacacgcta attcgtcttc cataaccatg ccgattaaga attatacata atgcgctgca
     7441 ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca
     7501 tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt tcttttgggt atgtttatct
     7561 gcgacactcg cagtaccgac gacatggagt aaaaatgtcc agaccaacta tcatcattaa
     7621 cgacctggat gccgaacgca tcgatattct gctggagcaa cccgcctatg ctggtttgcc
     7681 aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa atgtgttcgc cagaagagat
     7741 gccacacgac gtggtgacaa tgaacagccg ggttaaattc cgcaatctta gcgatggcga
     7801 agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc gatagcaata ctcagctttc
     7861 cgttatggct ccggtaggtg ccgcactgct ggggctgcgc gttggcgatt ccattcactg
     7921 ggaacttccg ggcggcgttg caacccacct tgaagtgctg gaactcgaat accagccaga
     7981 agctgctggc gactacctgc tttaatccca tctgcacaag cgataactcg ctttgcccag
     8041 aggatgcact cgcatcctct ttacgcaaaa tcagcaattt ttcctggttc tcactgttca
     8101 cttttgctgc aaaattattc cgtttcgttt gtgggaataa ccatggcaga agcattttac
     8161 atcctgatag gatttctgat tatggcggcg attattgtca tggctgtcct ttacctcgaa
     8221 aaccactcct gacgatcata ttgttccccc cggcatcgca ttcaccagac ctgaaaccgc
     8281 cttctgcgcc gcctctgcgc tttgtgcacc gggtaccaga atcaccttac ggcactcctc
     8341 ttcacgtttt tcgaaaatct catatccccg ggcggcctct tcaaacggca tatagtgggt
     8401 aacaatttct tctggtttca gtaatccttt ctcaattaac ggtaataatt ctcccagcca
     8461 tgcgtgaacg tgggtctgtc ccattttaaa cgtcaaccct ttatcaaagg cgtcgccaaa
     8521 caggaaaccg tgaataaatc cagcgtagac gcccggtacg ctaacaatgc cgccacgcct
     8581 gaccgccgca atacactggc gcaacgcttt accgctgctg ccctccagtt tcaggttagt
     8641 cagcaccgtt tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc
     8701 cacgccccgg tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc
     8761 gaaattaatc gggatcgcgc cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg
     8821 atcaacaaca aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc
     8881 cacaggacca gcaccataga ctgcaacgct tgaaccttgt tggatctgca cattttttgc
     8941 tgcctgccat gccgttggca gaatatcaga aaggaaaagt gctttatcat ctgaaagcaa
     9001 aggcggtact ttaaacggcc ccacattccc tttagggacg cggacatatt ccgcctgccc
     9061 gccaggaacg ccaccataca ggtgactata accaaacaat gccgcgggag caggtatctg
     9121 ttttttattg agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg
     9181 taatcgacag aaaaaacagt cgccacaagc aatgacgaac ggaattacca cccggtcgcc
     9241 tttttgcaaa ttttttacgt ccttcccggt ttcaacgact tcccccataa attcatgacc
     9301 aaaaatatcg ccatgtttaa cctggggtat tttgcctcga taaagatgga ggtcagagcc
     9361 acagatcgcc gttaccgtaa tacgcagaat aatatcatct gcctgttcaa tgcccggatc
     9421 gggaacattt tctacctgaa cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc
     9481 tccagagaca gttcaaaaaa gagtcagtct ttaagggtag ctatcacagc gggaaaacgg
     9541 gcggtaccgc aggttttggg agaattcctg gtgttcagtg agaatatatt aatcacagaa
     9601 accgttcgta aggaaaaaga accagacaaa tatcagggat aaatattata atcatggcga
     9661 atgatttgtt catgattaac agggagaaag ggttatgtat aagacaatca ttatgccagt
     9721 tgatgtattt gaaatggaat tgagcgacaa agctgttcgc cacgctgaat tcctcgccca
     9781 ggatgacgga gttattcatc tacttcacgt actacccggg tcagccagcc tgagcctgca
     9841 ccgttttgcc gctgatgtgc gtcgttttga agagcatctg caacatgaag cagaagaacg
     9901 tctgcaaacg atggtcagcc acttcaccat cgatccttcc cgcattaaac aacatgtccg
     9961 ttttggtagc gtgcgggatg aagtcaatga gttggcagaa gaactggggg ctgatgttgt
    10021 agttatcggt tctcgcaacc catcgatttc gacccatctg ttaggttcta acgcctcgag
    10081 cgtgatccgc cacgccaatc tgcctgtact ggtcgttcgc taacagataa aagtatttat
    10141 gccgccctgc ctggaacatc ccggcagggc tttttaatgc cataaaaaag ccgccaggtt
    10201 tgaccccggc ggctaagcaa ttgcaggtga atcttacttc ttcttatgca gttttggtgc
    10261 gaatcaggta gtcaaaagca ctcagagagg ctttggcacc ttcgccagtg gcgatgatga
    10321 tctgcttgta cggaaccgtc gtacagtcac ccgctgcgaa cacgcctttg acgttggttt
    10381 cacatttcgc atcaatgata atctcgccca tgcggttacg ttcgactgcg ccttccagcc
    10441 agttggtgtt cggcagcaga ccaatctgga caaaaatacc ggccagttcg atgttgtgaa
    10501 tatcgccgct gacacgatct cgatattcca gaccaacgac tttgctgccg tcgcctttca
    10561 cttccgtggt ttgcgcattc agaataatgt cgacgttttt caggctgcgc agtttgtcct
    10621 gcagaacctg gtcggctttc atttctggcg caaattccag cagcgttacg tgctcaacga
    10681 tacccgccag gtcgattgcc gcttccacgc cggagttacc gccgccgata accgctacgc
    10741 gtttgccttt aaacagcggg ccgtcgcagt gcgggcagta ggtcacgcct ttagtgcgat
    10801 actgatcttc gcccggaacg ttcatgttgc gccattttgc acccgtcgcc acgataatgc
    10861 tgcgtgcttt cagtaccgcg ccagaagctg tttcaatctg gtgtagacca ccttcaaccg
    10921 ctgccgggat cagtttgctg gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa
    10981 cgtgaacttt cagtgcgcct gccaacttct gcccttcagt cttcggtaca gaaatgtagt
    11041 tttcgatatc aacggtatcg aggatctgac caccaaaacg ttcgcccatc agaccggtac
    11101 ggatgccttt acgtgcggag taaattgctg ccgctgcacc cgccgggccg gaaccaacga
    11161 ttaatacgtc ataagcatca cgcttgttca gctcttctgc cgcacgtttt tccgcgccag
    11221 tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt
    11281 ttacgaacac tgccggaacg cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc
    11341 cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcaatac gctcatcagg ttcagcgcct
    11401 gcaccacgtc cgggcagtta tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac
    11461 cgtcaatatg gcgaatctgc tccagcagag actgcgcttc tttcgacgga tgaccaccgg
    11521 tccacagcaa cgccagtacc agcgaggtga actcgtggcc cagcggggag cctgcaaaac
    11581 gtggtccctg gttggaacct gggttggtga tcaggaaaga cggcttacgc actggcaagc
    11641 tgttatcttc tttaaaggtg actttgtctg acagttctgc gatttcagcc aacagttcct
    11701 tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat taactcaaca ggcttggtca
    11761 atttctcaag gtaagccttg agttgagttt tcatatttgt gtcgagcatg tttatctcct
    11821 aggctttaaa catcatcatg caagcggcct ctgcggctac ctggatgcag cttgcatcat
    11881 ggtgcgggga agaaaatcgg gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta
    11941 tttcgcgtca gagcaaggcg actagcctgt gaatccccgg gagcttacac aagtaagtga
    12001 ccggggtgag cgggcgacgc caacgccgct atggcgtgaa agacgaagga aatttagatt
    12061 ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg
    12121 caaacttcac ctgggtgaga agctacgtac tgagctgctt tgattttacg cagcagatca
    12181 gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg
    12241 tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta
    12301 cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca
    12361 gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct
    12421 acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat
    12481 acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt
    12541 tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg
    12601 gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat
    12661 ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct
    12721 tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc
    12781 tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc
    12841 tgaggtgttg cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag
    12901 ggcaatcacc tgatctaagc tcttacctat gacagtgata ggttatgcct tttactcgac
    12961 ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac tggctctgct
    13021 tcctgcaatc gccttcgcag aggaacttcc ttccccagta aaagcgattg aaaaacaggg
    13081 cattacaatc atcaaaacgt tcgatgcccc cggaggaatg aaaggttatc tcggaaagta
    13141 tcaggatatg ggcgtcacca tctacctgac tcccgatggt aagcacgcta tctctggtta
    13201 catgtacaac gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc
    13261 accagccgga cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa
    13321 aaaagatgcg ccggtcattg tctacgtctt cgccgatccg ttctgcccat attgtaaaca
    13381 gttctggcag caggcgcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt
    13441 ggttggggtt atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga
    13501 tcccgcaaaa acctggcaac aatatgaagc ctctggttgc aagcttaagc taaacgtgcc
    13561 tgcaaacgta agtacagagc aaatgaaagt gttaagtgac aatgagaaac tgatggacga
    13621 tctgggggca aatgtcacgc cggctatcta ttacatgagt aaggaaaata cgctgcaaca
    13681 ggccgtgggg ttgcccgatc agaaaacgct taatatcatt atgggaaata aataaaaggt
    13741 aattcatgga tggttgaact atatccctgc ttttcaacca tcctaaaaaa gcatgaattg
    13801 ctcgctctac cctaagtggc tgtgctattt tagaaaaact taatcctgag ttaagcaaaa
    13861 aaacataatc aataaaaata catacgcaaa aaacattgat taagtgaata taccatggaa
    13921 gaaaaatata aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta
    13981 aatcttcttg tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa
    14041 tcactatata tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt
    14101 aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttgaat
    14161 ctgcatcacc atcttgagaa aacctcaaag gccttgaaca aacaattaat atagtcaata
    14221 agtccgaact aaagaaaaac tttattatct acggccctca gctcatttct tgctctaata
    14281 atagtatgct gatccgctgc ctacggcaag atgcctctgt cgagattgag tgccatgata
    14341 tcctcatgtc agcagagaat gctgaagaac tgttggttca ccgcaaagcc gatctggtga
    14401 tcacccaaat gccagtgatc agccgttccg tcatttgcat gccattgcat acaattcgca
    14461 atactctaat ttgtagtaat aagcatccgc gaattactga caatagcact tatgaacaaa
    14521 ttatggcaga agagttcacc caattaatat ccaaatcagc aggagttgat gattttcaaa
    14581 tggaaataga cgagaagttt atgaatcgca aaatttcatt tcgtggttcc tctttgctga
    14641 caataattaa tagcattgca gttacagatt tactgggaat agtcccctat gaactttata
    14701 actcccaccg tgactttctt aatttaaaag agataaaact agaacatccg ttaccttcaa
    14761 taaagcttta catttcctat aataaatctt ctttaaataa tttggtgttc tcccgattta
    14821 ttgaccgtct aaatgagagt ttttgatata taactacaga cagctactat aactttcatc
    14881 tatttattca cagcgcgaat gaaaaataaa acactctgct ttacagtgtt tactttataa
    14941 ttaacagtct tatctcactg ttcaccctgt aacgttcaga gagcgtcttc agatgtctat
    15001 ttataaaatt cctcttccgc tcaatattct ggaagccgca cgagagcgta ttacctagac
    15061 act
//
LOCUS       DADKXM010000096        15029 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.097, whole genome shotgun sequence.
ACCESSION   DADKXM010000096 DADKXM010000000
VERSION     DADKXM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.097"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..237
                     /locus_tag="JQR05_002826"
     CDS             <1..237
                     /locus_tag="JQR05_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY5324950.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="JQR05_002827"
     CDS             247..885
                     /locus_tag="JQR05_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY5324951.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNSYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="JQR05_002828"
     CDS             888..2051
                     /locus_tag="JQR05_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY5324952.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3760
                     /locus_tag="JQR05_002829"
     CDS             2135..3760
                     /locus_tag="JQR05_002829"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY5324953.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASSPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="JQR05_002830"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="JQR05_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY5324954.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="JQR05_002831"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="JQR05_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY5324955.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="JQR05_002832"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="JQR05_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5324956.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGALMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="JQR05_002833"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="JQR05_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY5324957.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="JQR05_002834"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="JQR05_002834"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY5324958.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGKALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="JQR05_002835"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="JQR05_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY5324959.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="JQR05_002836"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="JQR05_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY5324960.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="JQR05_002837"
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="JQR05_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY5324961.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="JQR05_002838"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="JQR05_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY5324962.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11542
                     /locus_tag="JQR05_002839"
     CDS             10706..11542
                     /locus_tag="JQR05_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY5324963.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ECLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11542..12228
                     /locus_tag="JQR05_002840"
     CDS             11542..12228
                     /locus_tag="JQR05_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY5324964.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12357..12632)
                     /locus_tag="JQR05_002841"
     CDS             complement(12357..12632)
                     /locus_tag="JQR05_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5324965.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12959..13354
                     /gene="rpsF"
                     /locus_tag="JQR05_002842"
     CDS             12959..13354
                     /gene="rpsF"
                     /locus_tag="JQR05_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY5324966.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTVAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13361..13675
                     /gene="priB"
                     /locus_tag="JQR05_002843"
     CDS             13361..13675
                     /gene="priB"
                     /locus_tag="JQR05_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY5324967.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13680..13907
                     /gene="rpsR"
                     /locus_tag="JQR05_002844"
     CDS             13680..13907
                     /gene="rpsR"
                     /locus_tag="JQR05_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY5324968.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13949..14398
                     /gene="rplI"
                     /locus_tag="JQR05_002845"
     CDS             13949..14398
                     /gene="rplI"
                     /locus_tag="JQR05_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY5324969.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEVRRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14469..15026)
                     /locus_tag="JQR05_002846"
     CDS             complement(14469..15026)
                     /locus_tag="JQR05_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY5324970.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPNLWTQENGEWIYLRN"
BASE COUNT         3508 a         3656 c         4058 g         3807 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatagcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acgttttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtta
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cacgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tcttcgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatccagt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgttgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc taggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttcatctccc acagcacgcg
     6601 gatttcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggctttac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggggt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacattggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgct caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca atatgtgatt ggttttgatt aatcctgaca ctattttttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt
     7681 tcgttgtgtg aatcccactc tatccgtgtg gaattatttg cgggtcgcgc cacatttaat
     7741 cataaataat cttgttgtga ttgcttttga aaattagagt gagtgcacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg caaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgataattac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat ggattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgcgccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gcggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattttggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaactgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     9901 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactgacgag tgcctgtcgc gcctcgactg
    10861 gagccgcgag cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgca tggccaaagc
    11521 aggcatggtg gaggcggcat aatgcaaaag ctaaaacagc aggtgtttga agccaacatg
    11581 gatctgccgc gctatgggct ggtgaccttt acctggggca acgtcagcgc tatcgaccgc
    11641 gaacgcgggc tggtggtgat caagcccagc ggcgttgcct acgaaaccat gaaagcagac
    11701 gatatggtgg tggtcgatat gagcggcaag gtggtggaag gggagtatcg cccatcttcc
    11761 gacactgcga cgcatctcga actctaccgt cgttacccgt cgcttggcgg cattgtccat
    11821 acccactcca ctcatgccac cgcatgggcg caggcggggc tggcgatccc ggcgttaggc
    11881 accacgcacg ctgactactt ctttggtgat ataccgtgta cgcgtggatt aagcgaagaa
    11941 gaggtgcagg gtgagtatga actgaacact ggcaaagtga ttatcgaaac gctgggcaac
    12001 gccgagccgc tgcatacgcc gggaattgtg gtgtatcagc acggaccgtt cgcctggggg
    12061 aaagatgccc acgacgcggt gcataacgcg gtagtgatgg aagaagtggc gaaaatggcg
    12121 tggattgcgc gcagtattaa tccacaactc aatcatatcg acagctacct gatgaataaa
    12181 cacttcatgc gtaagcacgg tcctaacgct tattacgggc agaagtaaaa cacgcgctgc
    12241 ggaaatttcc ttcctcggga gataactggt ctaattccgc agccgttttt caaaaaaagc
    12301 cccctgcgaa gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag
    12361 cgataaatat ccgcgctggc gtgcatattg ccgttactcc ccggttcccg catcagaatt
    12421 acgtggtagt acgttgcgcc ttgcgcatct gtttcttcat ttaatgcctg acgtgcttcg
    12481 ctttcagtgg cgaaattatg attgatataa atcacgccta agctttgcac atcgtccata
    12541 tttctggcct ggtggttatt aatttcaatg gctgcccatg tatttgcact tagcaaaagc
    12601 acagccagaa gggctaaaac tcgactgaac atagatacct cctcgacggc tgactttgtg
    12661 tgctctcctc tgtgatgatc ttctgattta attttaatca atgataaaga agttgatggt
    12721 gaccatttct gatgcagttg ttcaaaaaaa cactgtgacg gaatgtgatg aacttcaaat
    12781 cagcggttta gaggttattt gcgaaagggg agatttattt ctgctccgcc cttgagttta
    12841 gcggggtatg tcagtactat aacgcgtcat ttttcagccg acctttaaca cgttccttgc
    12901 ctccccggga ttcggctgac ccagacagga ggctgaataa tccgtaagga gcaattcgat
    12961 gcgtcattac gaaatcgttt ttatggtcca tcctgaccag agcgaacagg ttccgggcat
    13021 gatcgagcgc tacactgttg ccatcactgg tgcagaaggc aagatccacc gtctggaaga
    13081 ctggggccgc cgtcagctgg cttacccgat caacaaactg cacaaagcac actacgttct
    13141 gatgaacgtt gaagctccgc aggaagtgat cgatgagctg gaaactacct tccgcttcaa
    13201 cgatgccgtt atccgcagca tggttatgcg taccaagcac gctgttaccg aagcatctcc
    13261 gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc
    13321 tgatgatgct gaagctgggg attctgaaga gtaatttctg atgaccaacc gtctggtgtt
    13381 gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg
    13441 ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc ggctttcacc ggcaggcatg
    13501 gtgtcaaatg cccgttattg ttagcggaca cgaaaaccag gccattactc acagtataac
    13561 ggtcggcagt cgcataaccg ttcaggggtt catttcatgc cacaaggcaa agaacggact
    13621 gagcaaaatg gttttgcatg ccgagcagat tgaattgata gattctggag actagccata
    13681 tggcacgtta tttccgtcgt cgcaagttct gccgtttcac cgcggaaggc gttcaagaga
    13741 tcgactataa agatatcgct acgctgaaaa actacatcac cgaaagcggt aagattgtcc
    13801 caagccgtat caccggtacc cgtgcaaaat accagcgtca gctggctcgc gctatcaaac
    13861 gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc
    13921 attaatacga ctttgagagg ataaggtaat gcaagttatt ctgcttgata aagtagcaaa
    13981 cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc tatgctcgta acttcctggt
    14041 accgcagggt aaagctgttc cagctaccaa gaaaaacatt gaattcttcg aagtacgtcg
    14101 cgctgaactg gaagctaaac tggctgaagt tctggcagct gctaatgctc gcgctgagaa
    14161 aatcaatgca ctggaaactg ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt
    14221 cggttccatc ggtactcgcg acatcgctga cgctgtaact gcagctggcg ttgaagtggc
    14281 taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc actggcgaac acgaagtgag
    14341 cttccaggtt cacagcgaag tattcgcgaa agtgatcgta aacgtagtag ctgaataatt
    14401 cgttattcaa cgagacgtaa aaacgccgac cattggtcgg cgttttgctt tctatttttc
    14461 gtcaggcatt aatttcgcaa gtagatccat tcaccattct cttgtgtcca taagttagga
    14521 tggtttgctg gcgttagatg catacgatca ctccttatgt ctcggtcctg cggcactcgc
    14581 tcgaaaccta aacccgagta gattgcggct gaatttgata aggaacgtag accaattttc
    14641 aaatcatggg gtaaatcaga atacgcgttt agcaatgcag caatgatgaa tccgagggca
    14701 ttagaaacgc ctgtaacagt gcgaagttgg tcgatataaa gatcgattgt tggtagttca
    14761 gatgaccagc aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg
    14821 gtatagtctg gagtactata accaggtccc aaaacaacct cgtgtattgt gtatttactg
    14881 ttgggttcga aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca
    14941 cccgcgtaag ctttattgta tttctgggcg tgaatattat ctaatgtagc ttcatggttc
    15001 catccaaaag aattatcacc attcattgt
//
LOCUS       DADKXM010000097        15010 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.098, whole genome shotgun sequence.
ACCESSION   DADKXM010000097 DADKXM010000000
VERSION     DADKXM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.098"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQR05_002847"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQR05_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY5324971.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADRGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQR05_002848"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQR05_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY5324972.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQR05_002849"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQR05_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY5324973.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMKLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQR05_002850"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQR05_002850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY5324974.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRLGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JQR05_002851"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JQR05_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY5324975.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JQR05_002852"
     CDS             complement(8217..9110)
                     /locus_tag="JQR05_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY5324976.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JQR05_002853"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JQR05_002853"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY5324977.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JQR05_002854"
     CDS             complement(10007..10189)
                     /locus_tag="JQR05_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY5324978.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JQR05_002855"
     CDS             complement(10241..11473)
                     /locus_tag="JQR05_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY5324979.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JQR05_002856"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JQR05_002856"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY5324980.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JQR05_002857"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JQR05_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY5324981.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..15010)
                     /locus_tag="JQR05_002858"
                     /pseudo
     CDS             complement(14278..15010)
                     /locus_tag="JQR05_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
BASE COUNT         3530 a         4223 c         3691 g         3566 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tcacttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 ccctctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtgg cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac ttcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctagacgca acactgacca cagggtctgt aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttcc
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagta tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccaa gggataatca gttgttgccc
    14941 gctatcccct tcaggctagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt
//
LOCUS       DADKXM010000098        14656 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.099, whole genome shotgun sequence.
ACCESSION   DADKXM010000098 DADKXM010000000
VERSION     DADKXM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.099"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..179
                     /gene="fliJ"
                     /locus_tag="JQR05_002859"
     CDS             <1..179
                     /gene="fliJ"
                     /locus_tag="JQR05_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="rod/hook and filament chaperone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis chaperone FliJ"
                     /protein_id="HAY5324982.1"
                     /translation="WTQKVDIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKK
                     MDEFAQRAAMRKPE"
     gene            176..1303
                     /gene="fliK"
                     /locus_tag="JQR05_002860"
     CDS             176..1303
                     /gene="fliK"
                     /locus_tag="JQR05_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY5324983.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            1408..1872
                     /gene="fliL"
                     /locus_tag="JQR05_002861"
     CDS             1408..1872
                     /gene="fliL"
                     /locus_tag="JQR05_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY5324984.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            1877..2881
                     /gene="fliM"
                     /locus_tag="JQR05_002862"
     CDS             1877..2881
                     /gene="fliM"
                     /locus_tag="JQR05_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY5324985.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPCDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            2878..2967
                     /gene="fliN"
                     /locus_tag="JQR05_002863"
                     /pseudo
     CDS             2878..2967
                     /gene="fliN"
                     /locus_tag="JQR05_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="One of three proteins involved in switching the
                     direction of the flagellar rotation; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            2958..3119
                     /locus_tag="JQR05_002864"
                     /pseudo
     CDS             2958..3119
                     /locus_tag="JQR05_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707832.3"
                     /note="with FlhA, FlhB, FliP, FliQ and FliR is one of the
                     membrane components of the flagellar export apparatus;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliO"
     gene            3119..3856
                     /gene="fliP"
                     /locus_tag="JQR05_002865"
     CDS             3119..3856
                     /gene="fliP"
                     /locus_tag="JQR05_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY5324986.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            3866..4135
                     /gene="fliQ"
                     /locus_tag="JQR05_002866"
     CDS             3866..4135
                     /gene="fliQ"
                     /locus_tag="JQR05_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY5324987.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            4144..4929
                     /gene="fliR"
                     /locus_tag="JQR05_002867"
     CDS             4144..4929
                     /gene="fliR"
                     /locus_tag="JQR05_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY5324988.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPTNDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCKHLFSEIFNLLADIISELPLI"
     gene            5219..5842
                     /gene="rcsA"
                     /locus_tag="JQR05_002868"
     CDS             5219..5842
                     /gene="rcsA"
                     /locus_tag="JQR05_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY5324989.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(5886..6128)
                     /gene="dsrB"
                     /locus_tag="JQR05_002869"
     CDS             complement(5886..6128)
                     /gene="dsrB"
                     /locus_tag="JQR05_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY5324990.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            6237..6464
                     /gene="yodD"
                     /locus_tag="JQR05_002870"
     CDS             6237..6464
                     /gene="yodD"
                     /locus_tag="JQR05_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY5324991.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            6762..7577
                     /locus_tag="JQR05_002871"
                     /pseudo
     CDS             6762..7577
                     /locus_tag="JQR05_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(7574..9272)
                     /gene="yedQ"
                     /locus_tag="JQR05_002872"
                     /pseudo
     CDS             complement(7574..9272)
                     /gene="yedQ"
                     /locus_tag="JQR05_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(9443..9625)
                     /locus_tag="JQR05_002873"
     CDS             complement(9443..9625)
                     /locus_tag="JQR05_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY5324992.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(9704..10621)
                     /locus_tag="JQR05_002874"
     CDS             complement(9704..10621)
                     /locus_tag="JQR05_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY5324993.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            10794..11714
                     /gene="yedA"
                     /locus_tag="JQR05_002875"
     CDS             10794..11714
                     /gene="yedA"
                     /locus_tag="JQR05_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY5324994.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(11703..12173)
                     /locus_tag="JQR05_002876"
     CDS             complement(11703..12173)
                     /locus_tag="JQR05_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY5324995.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(12154..13572)
                     /locus_tag="JQR05_002877"
     CDS             complement(12154..13572)
                     /locus_tag="JQR05_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY5324996.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(13639..14334)
                     /locus_tag="JQR05_002878"
                     /pseudo
     CDS             complement(13639..14334)
                     /locus_tag="JQR05_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(14374..14631)
                     /locus_tag="JQR05_002879"
     CDS             complement(14374..14631)
                     /locus_tag="JQR05_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5324997.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         3559 a         3900 c         3606 g         3591 t
ORIGIN      
        1 agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc
       61 aggcctggca gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc
      121 tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat
      181 tcgcttagcg cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc
      241 cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa gcattagcag gcgagacaac
      301 aactgacaaa gcggcccccc agttgctggt ggcaacagat aagcccacga caaaaggcga
      361 gccgctggtc agcgatattc ttgccgatgc gcaacaagcg gatttactga tccctgtgga
      421 tgaaacactt cctgtcatca acgacgaaca atccacatca acaccattaa ccaccgctca
      481 gacgatgacg ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga
      541 tcttaatgaa gacgtcaccg caagtctgag cgcccttttt gcgatgttgc cgggttttga
      601 caatacgccc aaagtgactg atgcaccgtc aaccgtgtta ccggcagaga aaccaacgct
      661 cttcacaaaa ctgacttctg cgcaactcac tacagcacag cctgatgacg cccccggcac
      721 accagctcag ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag
      781 cacgccttcg ccggtgaccg ctgacgccag cccgctaatc actccacacc agacacagcc
      841 actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg aatggcaaca
      901 atcattgagc cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg
      961 tctgcaccca caggatttag gtgaagtgca aatttccctc aaagtggatg ataaccaggc
     1021 gcagatccag atgatttcac cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc
     1081 ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acattagtgg
     1141 cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa cagcaaagcc aacgcacagc
     1201 aaaccatgaa cctctggcgg gggaagacga cgatacgctt ccggttcccg tctctttaca
     1261 agggcgtgta acaggtaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg
     1321 taatccgcgt cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc
     1381 agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag
     1441 cgatcgcttt ggatcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt
     1501 tacagctact ggcattcgca tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg
     1561 ccctccccgg tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc
     1621 gtgctttata tcggcataac cctgcgactg aaagatgaag caacccgctc gcggctgagt
     1681 gagtatttgc cggaagtccg tagtcgcttg ctgttactgt tttcacgtca ggatgctgcc
     1741 gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac cacactttcc
     1801 accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt
     1861 attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt
     1921 gaatggtgac agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat
     1981 tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga
     2041 aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg
     2101 tagcccggac attaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa
     2161 cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa ccgctacgcg gcacagggct
     2221 ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg
     2281 acgcttcccg accaaagtgg aaggtcgcga gtttacccat actgaacagc gcgtcatcaa
     2341 ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc tggaaagcga ttaatccgct
     2401 ggaagttgag tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc
     2461 gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt
     2521 taatatctgc ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc
     2581 gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca
     2641 gcattcacag ctggaactgg tggccaactt tgccgatatc tcgctacgcc tgtcgcagat
     2701 tttaaaactg aaaccctgcg acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca
     2761 tgttgacggc gtcccggttc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt
     2821 acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg
     2881 agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa
     2941 gcgttgagcg agcaaaaatc atggtcgatg tggaagatgc acggctggtg ctcggcgtta
     3001 ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaacg gaagagatac
     3061 cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa gcgtggcggg agatcctgat
     3121 gcgtcgtttg ttttctgtcg caccggtcct tctctggctg attacgcccc tcgccttcgc
     3181 gcaactgccg ggtatcacca gccagccgct acctggcggt ggacaaagct ggtcgctccc
     3241 ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt ccagcgattt tactgatgat
     3301 gaccagtttc acccgcatca ttattgtttt tggtttattg cgtaacgcgc tgggaacgcc
     3361 ctccgcgcca cctaaccagg tattgctggg gctggcactg tttttgacct tttttattat
     3421 gtcgccagtg atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat
     3481 ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca
     3541 gacccgcgag gcagatttag ggttgtttgc cagactggcg aataccggcc cgttgcaggg
     3601 gcctgaagct gtgccgatgc gcattttgct cccggcctac gtgaccagcg agttgaaaac
     3661 cgcatttcag ataggcttca cgattttcat cccttttttg attatcgacc tggtgattgc
     3721 cagcgtgttg atggcattgg ggatgatgat ggttcccccc gccaccattg ctctgccctt
     3781 taaactgatg ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca
     3841 gagcttttac agctagagag gcaaaatgac acctgaatcg gtcatgatga tgggaactga
     3901 agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg ctggtagcgc tggtcacggg
     3961 ccttatcatc agtattttgc aggcagccac gcagattaac gaaatgacgc tgtcgtttat
     4021 tccgaaaatc atcgccgtat ttatcgccat cattattgcc ggaccgtgga tgctcaatct
     4081 gttgctggat tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtagcccgt
     4141 acaatgatgc aggagacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg
     4201 ttactgcgcg tgctggcgct catttctacc gcgccaattc tgagcgaacg cagcgtgccg
     4261 aaacgggtaa aactgggtct ggcaatgatg atcacgttcg ccattgcccc atcattacct
     4321 accaacgatg ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg
     4381 atcggcattg cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc
     4441 gaaattatcg gtctgcaaat ggggctgtca tttgcgacat ttgtcgatcc tggcagccat
     4501 ctcaatatgc ccgttttagc gcgcatcatg gatatgctgg cattactgct gttcctgaca
     4561 tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac cctgccgatt
     4621 ggtagcgaac cgttgaacag caatgcgttt ctggcaccca ccaaagcagg gagtttgatt
     4681 ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgttgctgac cctgaatctg
     4741 gcattaggtt tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca
     4801 ttaactctga ctgtcggcat ctctttaatg gcggcattaa tgccgttaat tgcgcctttt
     4861 tgcaaacatt tattcagtga aatttttaat ttgctggctg atattattag tgaattacca
     4921 ttaatataat tccgtaacgt ttatcatgct accctaagga ttatccgaaa aataatatct
     4981 gcgaacatct tccaggatac tcctgtagcg aaatatttgt tttaagctca ctcacatatc
     5041 gcaacattta ctttacttta agacaattcc aggcaaatta tacaacactt tacgggatag
     5101 taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg ttccgtttag
     5161 tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag ggtatgccat
     5221 gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg ggtatctgtt
     5281 gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg accttgccat
     5341 agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt tcatccacga
     5401 tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata cgttatttat
     5461 cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca gaaaaaattt
     5521 attgatcagc tctaaatcga ttaaaccgga atctctcgac gatatccttg gcgatattct
     5581 gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat tgagccgaac
     5641 cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa tctccgacca
     5701 aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac gtaagatcaa
     5761 aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg tgactaatgg
     5821 tatttttgtc aacatgcgct aacacattct gactggtggt ttccctccag tcaggctgaa
     5881 taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc attaaagaac
     5941 cagatgccga gcgggtagtc ttccagcgaa accaggtaca ttgtgccttc actaaactcc
     6001 tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac tgttacccga
     6061 tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt
     6121 tctggcagcg ccaggcgcgc gcgagtgctg attttctcga cggtctatac ttaagagatg
     6181 ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga aagatgatga
     6241 aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc
     6301 tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag aacgattctg
     6361 aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg gatgccttcg
     6421 aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa
     6481 tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt aaaaaattcg
     6541 ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg ctgacaagaa
     6601 aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc
     6661 cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc
     6721 tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca attcaacaac
     6781 cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt tatgactggc
     6841 aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc attctctgta
     6901 gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta caaggcttac
     6961 cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag gatatagacg
     7021 gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt ttaaatacgt
     7081 tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat gcaaccattg
     7141 ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat gaggcgtcgg
     7201 taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct cgtctgaacg
     7261 aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat gcctctgccg
     7321 gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca ggcagacgcc
     7381 caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag gtaatggatt
     7441 acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat gaggatccgg
     7501 cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt
     7561 ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg gcctgttttg
     7621 cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa tcaccggttt
     7681 cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc ttggcgatca
     7741 gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca gcctgcgtca
     7801 gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg gcaacgtcct
     7861 gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg tcgcccgcct
     7921 gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga atgacagaaa
     7981 aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg aacagtgcgc
     8041 cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac gagctttgca
     8101 gaacatacat gttgctgacc atccggcgaa tcacatacca ggagaggagt aacatggtgg
     8161 taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc
     8221 cttcgcttag cgtatggacg cgcaccagca caccgtcgaa atggtccaga cgttcccagc
     8281 taacatagcg actgtccata cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca
     8341 gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga ttggaagagg
     8401 tcaaaaatct cagcttactg tcatagagct gatactcacc atcgaggttt ttatcgatgg
     8461 cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc acgccatacc
     8521 agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta ttgctggcgt
     8581 gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc
     8641 cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg cgcgtaaaca
     8701 aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc tgttcaacca
     8761 tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca gcggtaattt
     8821 cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact aatgcatccg
     8881 atacgccatt gactggcagg gtgcgccgtc ggttgagttc gatttgccag gcgtgctcgt
     8941 cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc agtggcgcca
     9001 cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgttc gttatactgc
     9061 aaatgcttat cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag
     9121 gcatcttcca gcaccaccac ttcacgccag gtgagcaagg tggaaaaaag caataccacg
     9181 ataaagcaga gattaacgac atgaccgggc cccaggcggc gtgcgagttt tttcaaccag
     9241 ctctggtttt ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat
     9301 gattctggca taacccggcg atagcgtacc gcaaaacagc gataatttta taatggtggc
     9361 atgataagac gcgactatca ttcattgcag gcattgggac accaggccgg atgtgatccg
     9421 gcctggtgtt aatgataccc tttcaaactt cttccgcaga acgactcccc tcccccggaa
     9481 caagatcggt ttcatgaaaa gcttcccgct tgaccccgta accgtcatac caacgacact
     9541 caaccatacc gctggagtat ccagtgacaa tcatccgcgg gccgccctct ttaaccgtaa
     9601 cttcctcact aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc
     9661 ttagttgaag atggcgaatt ttgcataata gccagtgcga gtattaatgt gcctgaccgc
     9721 gcattttcgc tactgctttc actcccagca ccacgatgcc gccgatgata aatccaagaa
     9781 tcagatttaa aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgatat
     9841 gttcaatagc gtgatgcagc ggcgcaatac catgtaccac aatcccgccg ccgacgagga
     9901 acatcgccag cgtgccgaca atcgataatg ctttcatcag ccaaggcgcg ataatcaata
     9961 accctttacc taatgcctgc atcagcgcgc tggatttttc cgccagccaa taccccaggt
    10021 catcaatctt aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga
    10081 tgcctgaaag caccagcacc tgattaagca acggcgcctc tgccacaatc cccagcgtga
    10141 tggcgacgat ttccgcagac aaaataaaat cggtacgaat cgccccttta attttgtcct
    10201 tttcaaactt cagcggatcc tgcgccgcca gcttctccag acgctgctgg ctctgcgctg
    10261 gatcttcttt atgtttacgc gcctccagca tatgcagcac tttctctacc ccttcaaagc
    10321 agagaaacgc gccaccaatc atcaacagtg gcgtaatcgc ccacgggata aacgcactga
    10381 tgatcagcgc cagcggcacc agaatcactt tattgatcag cgatcctttc gccacgcccc
    10441 agaccacggg aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata
    10501 aatcatcccc taatacaccg gcggttttct tcgccgccag tttgcccatc acagagatat
    10561 cgtccagcag tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca
    10621 ttccctctca tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata
    10681 cttgcttcac aggtcaacat ttatttaaca atcaagtgca attatatctc tcgccagcag
    10741 aatgattttc acgtttacta tgaaccacct ttttatttcc gccgtgaggg attatgcgtt
    10801 tccgccagtt gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa
    10861 cctattttgt cattcggatt ggcgtgggaa gctggcctcc gttaatgatg gcgggcgttc
    10921 gattcctggc agccggtatt ttattgctgg catttttgct actgcgcgga cacaaacttc
    10981 ccccgctacg tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta
    11041 atggcatggt gacggttgcc gaacatcaaa atgttccttc cggcatcgcc gccgtagtga
    11101 ttgcaaccgt gcccctcttt accctgtgct tcagtcgcct gtttggcatt aaaacgcgca
    11161 aactggaatg ggtgggtatt gccattgggc ttgccggaat catcatgctc aatagcggtg
    11221 gaaatttaag cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg
    11281 cgtttggctc agtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga
    11341 ttgagatgct ggcggcaggc gtggtgttaa tgatcgcatc gatgattgcg ggtgaaaaac
    11401 tgacggcgct cccttccctg tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt
    11461 cgattatcgc cattaacgct tatatgtatt taatccgtaa tgtcagtccg gctctcgcca
    11521 ccagctacgc ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggtggcg
    11581 aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat tgttttcgcg gtggtactgg
    11641 tcacgttggg aaaatatctc ttcccggcca aacctgttgt cgcgccagtt attcaggatg
    11701 catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag
    11761 atccactctt ccagacgctc ggtaagcgcc gcatccgtca gcttctcgcg cctacgtaac
    11821 gcacactccc agacaatcag tacgcgccag ccgagctcct gcaagcgact gatatcgcgg
    11881 cgatcgcgtt caacattttt acctatcttc tccagccaga actcagttcg agtcgcaggc
    11941 actttaaaca gatagcagtg atgatgatgc cagaagcagc cgtgggtaaa tatcacgcag
    12001 cgatattcat caacgacaaa atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac
    12061 gcaaggccct gcccggttaa caaactggcg aggcgcttct caatcgccgt atcacgcgtg
    12121 gcaatcgcgc gcatattttt gctgcgggtg gccttatcgt gaacgtcgac catgttgtgc
    12181 ctcttgctga cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc
    12241 ggcaaagacc ggcacgacca ccgagttgcc gaactggcga taggcctgag tgtccgaaac
    12301 cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcacg
    12361 aggcgttaac cgacgtggac gatgttgctg attcagcgga tcgtcaaagt ctttctcacc
    12421 cgtggccata tcccaaccgc gatcgattaa aatttccgcg ccatctttgt aataacgagc
    12481 agacagcgta cgggtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc
    12541 gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt
    12601 caggatatat ttcgcctcaa ccatcgggtc cagcagctgc gccagcgtca ctcgctgtgc
    12661 agggaaacat tcgctgatat cacgcagagt aaaatcggct ttcagattca gatcgcgacg
    12721 aaaacccacc agcacgatgc gttcacggtg ctgcggcagg aaatgtttac catcgatgat
    12781 tttcggatcg tccggcccgt tatcttctgc atcggcaacg tcatagccca gttcgtccag
    12841 cgtctgcatg atgatgcgga acgtttttcc cttatcgtga ctcttcaggt ttttgacgtt
    12901 ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg cgcaccacat caaagaacag
    12961 cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac
    13021 gccagccagc gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat
    13081 gtgttgacga atatgttccg ccgccgcctc atcactcacg ccttcttgat gactgagggt
    13141 gatgtcgcgg atatcttcat taaaatgatg cgtcgccggg tcgcaataat ggttggcttt
    13201 ataagtgcgt accgcatgtt tgttccattc gctggtaaac acgcactgtc caccaatcga
    13261 ttcaaaacca cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata
    13321 atgcggatga tgttccggtg gtttgggtaa caacgtttgc agatgggcaa actctttctc
    13381 acttaaacgg tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca
    13441 gtgattctca cccacaccat taagctgcgc caccagcatt ttaacatcat aaatttgcag
    13501 cagtttctcc agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat
    13561 attttcctgc atagatttaa ccggccatct gagatggccg acaggttaac ataattactg
    13621 ccgtttaggg ccacgcgctc agccagcggg tgaaaacgca tctatcacct tgtgatcgat
    13681 accttcattc tcccccgcca gttcggcacc gagcttcgcc ataaactcga ctaaaaattg
    13741 cgcgttgtgc tgtgccagct gcttgcctct tgcggtttgc atggtttgcg gtaatttcag
    13801 taactttgtc tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc
    13861 gaacggatct tcgccatcaa acagcgccaa gcccaatgcc cctgaaacgg caaatacgcg
    13921 tgccaggcca atcgccccca gggcttccag ccgatccgca tcctagacga ttttagcttc
    13981 cgttgttaag ggggcgattt gcgcgctgaa actgtgagcg gcaatgacat gacaaacggc
    14041 ctcaattttc tccgccggaa attgcacgaa ctcttcacgg agcaggcgac gcgtctcttc
    14101 tgccgccagg attgaagaac gctgccgttg cggatgattt ttcgccaggc tcacgatatc
    14161 gtgaaaataa caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagttt
    14221 ctgtgccgta gcccagacac ggcgaaaatg acacaggtca tgcgccgcgt cctgatgttg
    14281 atgatggttt ttcaaccagt tctcgaactg tgcctgccag tgttgtaact ccatacgcgc
    14341 tccgttgcca aagagtcgca accttagcag tttttattca tagcgtctgc aacgtgcggg
    14401 acgaggtcgg gtgtaccatg caatgccgcc taacaaggca ccaaccaggg ccataaacag
    14461 aaatccgacc aatgctccta accacttccc cgcggtagat cctaaatcac tttcaaatcc
    14521 cgacacgggg gcatcgggta tccggctcat cacccagatg tatcgcgcca ttgcccagac
    14581 gaaatagcca caccatgtat agaaagccca caatgaatcg ccacggataa tctagacact
    14641 tccgagccgt tgataa
//
LOCUS       DADKXM010000099        14592 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.100, whole genome shotgun sequence.
ACCESSION   DADKXM010000099 DADKXM010000000
VERSION     DADKXM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.100"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(178..1371)
                     /locus_tag="JQR05_002880"
     CDS             complement(178..1371)
                     /locus_tag="JQR05_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5324998.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1507..3231
                     /gene="iucA"
                     /locus_tag="JQR05_002881"
     CDS             1507..3231
                     /gene="iucA"
                     /locus_tag="JQR05_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY5324999.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3232..4179
                     /gene="iucB"
                     /locus_tag="JQR05_002882"
     CDS             3232..4179
                     /gene="iucB"
                     /locus_tag="JQR05_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY5325000.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4179..5921
                     /gene="iucC"
                     /locus_tag="JQR05_002883"
     CDS             4179..5921
                     /gene="iucC"
                     /locus_tag="JQR05_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY5325001.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5918..7255
                     /gene="iucD"
                     /locus_tag="JQR05_002884"
     CDS             5918..7255
                     /gene="iucD"
                     /locus_tag="JQR05_002884"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY5325002.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7261..9456
                     /gene="iutA"
                     /locus_tag="JQR05_002885"
     CDS             7261..9456
                     /gene="iutA"
                     /locus_tag="JQR05_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY5325003.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10400..14287
                     /gene="sat"
                     /locus_tag="JQR05_002886"
     CDS             10400..14287
                     /gene="sat"
                     /locus_tag="JQR05_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY5325004.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNASNANFRYSF"
BASE COUNT         3892 a         3497 c         3639 g         3564 t
ORIGIN      
        1 cgggatcata ggttgttgca cgaaagaatc atggagatta caccgcagca actggttgaa
       61 ttcactatac ctgatgggcg ggggcagcaa gccagaacac tgcctgacat gctttcatca
      121 gacgcaggtc ctctctcata tcctctgatt tactccagta aggtgatgat catatcttta
      181 tttgatagta cggtaatgcc agataaatac agacacaata gtaaataata tagttgcaaa
      241 taatgctgcc ataatcagcg cgattgcgac acctgttttc gataccagcg gcaccagcaa
      301 agcgcacagt ccatagccaa gtgtgtgact ggtagcgagc cagcccgcgc ctgcgccatc
      361 agccagctta tcattcagta gaagttgata agcgggtgtt gccagagctg ctccaaatga
      421 cagcactgca cagcccgggt aaaacaacca tagctgttcg gagagcatga tagccagacc
      481 accactcatc aacactccgg cactcaggag cagggccacc ggagtcaggc gctgcggacg
      541 gagtaccccg aactgcgcga taagcgcagc tactgcggac agacccaaca gccaggccac
      601 ctgttggcta atggcggtgg tatccgtagc gaactggcga gtaagggcag gcgaaagtcc
      661 aagctgcatc atgctcaccg ccgcagccag taatattgcg caaagcagat aaggcagaca
      721 atcccgcttc agactgacgc tcttgcactc cggtgtgggc tgtggtggtg tgccgggcaa
      781 ccgcagcagc atcagcagcg ccagcactgg ggctgccatc agcagcccca gtggcgccag
      841 aggatgaatg gccagcattg ctgccgcgca cagcggcccg aatagcctgc cgcaactcag
      901 gccggagctg atggttgcca gagcggccat gcgattccct tctccagctc tctgcaatgc
      961 ccagacctga caggctggca ccattgccga cacggtcagc ccgtaggcga tccgggcgat
     1021 gattaatatt cccagtccca ccgctgttgt tatggcgctg gtagccatca gcacgcttcc
     1081 cagccctagc agggtaaagc ttatgccata tcctcccaga gcccacaaca ccacaggttt
     1141 actgccagta cgggaaattt gctttcccca ccacggggac gatggcagaa acagcattga
     1201 gccaagcatc agcagggcag cccatacaga cagactcaga tttgtctgta tgaccagaac
     1261 agggagcaca accagcagac cgttctgtcc gataccgaga agcccggcac tgaacgcaag
     1321 tggccagcag gacagtggtt tttggggcgt atcttcgatc cctggtgaca ttataatgtt
     1381 tcaactccat gtattaatta tgtttatttg taaaattaat ttatctgaca ataacattca
     1441 ttgttgataa tgagaatcat tattgacata attgttatta ttttactgtg tgggagctgt
     1501 ttgactatga ccctgccctc tgaaaaacca gccacagatg tggctgcgca gtgcttcctg
     1561 aatgcactga ttcgtgaaac cacagactgg aaactgacag aatacccgcc agacgaattg
     1621 ctcatcccgc tggatgagca gaaatcgctc catttcagag tggcttattt ctccccaacc
     1681 cagcatcacc gctttgcatt tcctgcacgt ctggtcacgg catcaggcag ttatcctgtc
     1741 gactttacca ctctctcccg gcttattatt gataagctac gccatcaact gtttctgccc
     1801 gttcccctct gcgaaacttt ccaccagcgc gtgctggaaa gccatgtcca tacgcaacag
     1861 gcaattgatg cccgtcatga ctgggccgct ctgcgtgaaa aagcgttgaa ttttggcgag
     1921 gctgagcagg cgctgctgac aggacacgct ttccaccctg cgcctaagtc tcatgaaccg
     1981 tttaaccggc gggaggctga acgctacctg cctgatatgg caccccactt cccactgcgg
     2041 tggttttcgg tggataaaac gcaaatcgcc ggtgaaagtt tgcatctcaa ccttcaacag
     2101 cggctgacgc gatttgccgc agagaatgcg cctcagttac tcaacgaatt aagtgacaac
     2161 caatggctgt tcccgttgca cccgtggcag ggggaatatc ttttgcagca ggggtggtgc
     2221 caggcacttg ttgctaaagg gctgattaaa gacttaggtg aggccggcac gtcgtggctg
     2281 ccgaccacct cttcccgttc cctctactgt gccaccagcc gcgatatgat caagttctcc
     2341 ctgagcgtac ggctgaccaa ctccatccgt actctgtccg tgaaagaagt gaagcgtggg
     2401 atgcgcctgg cacgcctggc tcaaaccgac ggctggcaga tgctacaggt ccgcttcccg
     2461 actttccggg taatgcagga ggatggctgg gccgggctgc tcgatcttaa cggcaacatc
     2521 atgcaggaaa gtctgtttgc cctgcgtgaa aatctgctgg tggatcagcc gaaaagccag
     2581 actaacgtac tggtctccct gactcaggcc gcacctgatg gcggtgattc gctgctggtt
     2641 tcggcggtaa aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc cgcccatgca
     2701 tgggtcgatg catactgtca gcaagtgcta aagccgctgt ttacggctga agcggattac
     2761 ggcctggtgc tgctggcgca tcagcaaaat attcttgtcc agatgcttgg ggatctgccg
     2821 gtcggattta tttaccgtga ctgtcagggc agcgctttta tgcctcacgc gacagactgg
     2881 ctcgattcta ttggcgaggc gcaggcggaa aatattttca cccatgagca gttgctgcgc
     2941 tatttccctt attacctgct ggttaactcc acttttgctg tgaccgctgc gctgggggct
     3001 gccgggctgg acagcgaatc gaatctgatg gctcgtgtac gagcatcgct ggctgaagtg
     3061 cgtgatcagg tgactcataa aacctgtctc aactacgtgc tggaaagtcc gtactggaac
     3121 gtaaaaggta actttttctg ttatctgaac gatcataacg agaataccat tgttgaccct
     3181 tcggtgatct atttcgattt cgctaacccg ctgcaggctc aggaggtctg aatgtctgag
     3241 gcaaacatta ttcacagcag atatggactg cgctgtgaaa aactcgacaa gcccctgaat
     3301 cttggctggg gactggacaa tagcgcggtg ttgcactgtc ccggggagct gccgacaggg
     3361 tggctgtgcg atgcgctgga tcagatattt atcgccgcac cacaactgtc agcagtggct
     3421 ctcccctggg ccgaatggcg tgaggagcca caggcgctga cgctttttgg gcaggtaaaa
     3481 agcgacatta tccatcgtac tgccttctgg cagttaccgt tatggttgag ttcaccggca
     3541 aaccgggcct ccggtgaaat ggtttttgat gcagagcgtg agatttattt cccgcagcgc
     3601 ccaccccgtc cacagggtga ggtttaccgc cgttatgatc cacgtattcg caggatgctg
     3661 agtttccgca ttgccgatcc cgtttctgat gcagaacgtt tcacccgctg gatgaatgat
     3721 ccgcgcgttg agtatttctg ggagcaaagt ggctcactgg aggtacagac cgcctatctg
     3781 gagcgccagc tcaccggtaa acatgcattc ccgctgattg ggtgcttcga cgatcggcca
     3841 ttcagctatt tcgaaatcta ctgggcggcg gaagaccgca ttggtcgtca ctattcgtgg
     3901 cagccctttg accgtggcct gcatctgctg gttggtgaac agcaatggcg cggggcccac
     3961 tatgtgcaaa gctggctgcg cgggctgaca cattacctgc tgctggatga gccccgtacg
     4021 cagcgcacag tactggagcc acgcaccgat aaccagcgcc tgttccgcca tcttgagcct
     4081 gcgggatacc ggacaattaa agagttcgac ttcccacata agcgctcgcg catggtgatg
     4141 gcggatcgtc atcacttctt cacggaggtc ggtctgtaat gaatcacaag gactgggatt
     4201 ttgttaaccg tcggctggtg gcaaaaatgt tgtctgagat ggagtatgag caggttttcc
     4261 acgcggaatc tcagggcgat gaccactact gcattaacct gccgggagca caatggcgct
     4321 tcatcgctga acgtggtatc tggggctggc tgtggattga tgcccaaacg ttgcgctgca
     4381 cggacgaacc agtactggcg cagacgctgc tgatgcagct aaagccggtg ctgtcaatga
     4441 gtgatgccac tgttgcagag catatgcagg atttgtatgc cacgctactg ggcgacctgc
     4501 aactgctgaa ggcccgtcgc ggactgagtg ccagtgacct gattaatctt gatgccgatc
     4561 gtctgcaatg cctgctgagc ggtcatccta aattcgtttt taataaaggt cgccgtggct
     4621 ggggcaaaga ggcgctggaa cgatatgcgc cagagtatac caacaccttc agactgcact
     4681 ggctggcggt aaaacgtgaa catatgatct ggcgctgcga taacgatctg gatattcagc
     4741 agttgttgac tgccgcaatg gatccgcagg agtttacacg cttcagtcag gtctggcagg
     4801 aaaacgggct ggatcacaac tggctgccgc tgccggtaca tccgtggcag tggcagcaaa
     4861 aaatcgccac agacttcatc gctgattttg ccgaaggcag gatggtgtct ctcggcgagt
     4921 ttggcgacca gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc agccgccggg
     4981 gagggctgga tatcaagctg ccgctgacca tctacaatac ctcatgctac cgggggatcc
     5041 ctggcagata tatcgctgcc ggaccactgg cttcacgctg gctgcaacag gtttttgcga
     5101 ccgacgccac cctggtgcaa agcggcgcag tgatccttgg tgaaccggct gcaggctatg
     5161 tgtcccatga aggctatgcc gcgcttgccc gggctcccta tcgctaccag gaaatgcttg
     5221 gtgttatctg gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt cctgttctga
     5281 tggcaacact gatggagtgc gacgaaaaca atcagccgct ggcaggcgca tatatcgacc
     5341 gctccggact ggacgctgaa acctggctta cgcaattgtt ccgggtggtt gtggttccgc
     5401 tgtatcacct gctttgccgc tacggtgtcg cgcttattgc acacggacaa aatataacgc
     5461 tcgccatgaa agagggggta ccacagcgtg ttctgctgaa agacttccag ggcgatatgc
     5521 ggctggtgaa agaagcgttt cccgaaatgg actctttgcc tcaggaggtg cgtgatgtca
     5581 catcccgtct gagtgcggac tacttaatcc atgatttgca gacgggtcac ttcgtgacag
     5641 tactgcgttt tatttcgcca ctgatggttc gtcttggcgt acctgaaagg cgattttatc
     5701 aactgctggc agcagtgttg agtgattaca tgaacaaaca tccacaaatg gcagagcgtt
     5761 ttgcgctttt ctcactcttc aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac
     5821 tgacctggcc ggacctggat ggcggcagtc gcatgctgcc gaattacctt gagaatctgc
     5881 aaaatccgct gtggctggta actcaggaat atgaatcatg aaaaaaagtg tcgattttat
     5941 tggtgtcggg acagggccat ttaatctcag cattgctgca ttgtcacatc agatcgaaga
     6001 actgaactgt ctcttctttg acgaacatcc tcatttttcc tggcatccgg gtatgctggt
     6061 accggattgt catatgcaga ccgtctttct gaaagatctg gtcagtgcag ttgcacctac
     6121 aaatccctac agctttgtta actatctggt gaagcacaaa aagttctatc gcttccttac
     6181 aagcagacta cgtacagtat cccgtgaaga gttttctgac tatctccgct gggctgctga
     6241 agatatgaat aacctgtatt tcagtcatac cgttgaaaat attgatttcg acaaaaaaag
     6301 tcgattgttt ctggttcaga ccagtcgggg agaatatttt gcccacaata tctgccttgg
     6361 tacaggaaaa caaccttatt taccaccctg tgtgaagcat gtgacacaat cctgtttcca
     6421 tgccagtgaa atgaatcttc gtcggccgga cctgagtgga aaacggataa ccgtggttgg
     6481 tggaggacag agtggtgcag acctgttcct taatgcatta cgcggggaat ggggagaagc
     6541 ggcggaaata aactgggtct cacggcgtaa taattttaac gcactggatg aggctgcttt
     6601 tgctgatgag tattttacac ctgaatatat ttcaggcttc tccggactga aggaagatat
     6661 tcgccatcag ttactggatg agcagaaaat gacatcggat ggcatcactg ccgattcttt
     6721 actgaccatt tatcgtgagt tgtaccaccg ttttgaagtt ctgagaaaac caagaaatat
     6781 ccgtctgcta cccagtcgct cggtaacaac tctggaaagt agtggtccgg gctggaggtt
     6841 attgatggaa catcatctgg atcagggcag agagagcctg gaaagtgatg tggtgatttt
     6901 cgccacaggt taccgttctg cattgccaca aatacttccc tcactgatgc ccttgatcac
     6961 catgcacgat aagaacacct ttaaagtgcg tgatgacttc actctggaat ggagtggccc
     7021 gaaagaaaat aacatctttg cggtcaacgc cagcatgcaa actcatggca tcgccgaacc
     7081 ccagctcagc ctgatggcct ggcgatctgc acgtattctt aatcgcgtac tggggcgtga
     7141 tttattcgat ctcagtatgc cgcccgcact gattcagtgg cgcagcggca gccggaaaaa
     7201 accgcagccg gaggctgctt ctttaactca ctatacagca aatattcagg aataacgatg
     7261 atgcgcaaaa agtatatgcc ccgggctctt ggtccgctgc ttcttgtcgt gctgtcacca
     7321 gctgtcgccc agcaaaacga tgataatgag atcatagtgt ctgccagccg cagcaatcga
     7381 actgtagcgg agatggcgca aaccacctgg gttatcgaaa atgccgaact ggagcagcag
     7441 attcagggcg gtaaagagct gaaagacgca ctggctcagt taatccccgg ccttgatgtc
     7501 agcagccaga gtcgaaccaa ctacggtatg aacatgcgtg gccgcccgct ggttgtcctg
     7561 attgacggtg tgcgcctcaa ctcttcacgt tccgacagcc gacaactgga ctctgtcgat
     7621 ccttttaata tcgaccatat tgaagtgatc tccggcgcga cggccctgta cggtggcggg
     7681 agtaccggag ggttgatcaa catcgtgacc aaaaaaggtc agccggaaac catgatggag
     7741 tttgaggctg gcacaaaaag tggctttaac agcagtaaag atcacgatga gcgcattgcc
     7801 ggtgctgtct ccggcggaaa tgaccatatc tccggacgtc tttccgtggc atatcagaaa
     7861 tttggcggct ggtttgacgg taacggcgat gccaccctgc ttgataacac ccagaccggc
     7921 ctgcagcact ccaatcggct ggacatcatg ggaaccggta cgctgaacat cgatgaatcc
     7981 cggcagcttc aactgataac gcagtactat aaaagtcagg gagacgacaa ttacgggctt
     8041 aatctcggga aaggcttttc cgccatcagc gggagcagca caccatacgt cagtaagggg
     8101 ctgaattctg accgcattcc cggcactgag cggcatttga tcagcctgca gtactctgac
     8161 agtgatttcc tgggacagga actggtcggt caggtttact accgcgatga gtcgttgcgg
     8221 tactacccgt tcccgacggt aaatgcgaat aaacaggcga cggctttctc ctcgtcacag
     8281 caggataccg accagtacgg catgaaactg actctgaaca gccaactgat ggacggctgg
     8341 caaatcacct gggggctgga tgctgagcat gagcgcttta cctccaacca gatgttcttc
     8401 gatctggctc aggcaagtgc ttccggaggg ctgaacaacc ataagattta caccaccggg
     8461 cgctatccgt catatgacat caccaatctg gcggccttcc tgcaatccag ctatgacatt
     8521 aatgatattt ttaccgttag cggtggcgta cgctatcagt atactgagaa cagggtagat
     8581 gatttcatcg actacacgca gcaacagaag attgctgccg ggaaggcgat atctgccgac
     8641 gccattcctg gtggttcggt agattacgat aactttctgt tcaatgctgg tctgctgatg
     8701 cacatcaccg aacgtcagca ggcatggttc aatttttccc agggggtggc attgccggat
     8761 ccggggaaat attatggtcg cggcatctat ggtgcagcag tgaacggcca tcttcccctg
     8821 acaaagagcg tgaacgtcag cgacagtaag ctggaaggcg tgaaagtcga ttcttatgaa
     8881 ctgggctggc gctttatcgg tgacaacctg cggactcaaa tcgcggcata ttactcgctt
     8941 tccaataaga gcgtggaaag gaataaagat ctgaccatca gtgtgaagga cgacaggcgc
     9001 cgtatttacg gcgtggaagg tgcggtggac tacttgatcc cggatactga ctggagtacc
     9061 ggtgtgaact tcaatgtgct gaaaaccgag tcgaaagtga acggtcaatg gcaaaaatat
     9121 gacgtgaagg aatcaagtcc atcgaaagcg acagcttaca ttaactgggc gccggaaccg
     9181 tggagtctgc gtgtacagag caccacttct ttcgacgtaa gcgatgcaga gggtaacgat
     9241 attaatggtt acactaccgt cgattttatc agtagttggc agcttccggt gggaacactc
     9301 agcttcagcg ttgagaacct cttcgaccgt gactatacca ctgtctgggg acagcgtgca
     9361 cctctgtact acagcccggg ttacggccct gcttcactgt acgactacaa aggccggggc
     9421 cgaacctttg gtctgaacta ctcagtgctg ttctgacggg gatgcttttg caacaagggt
     9481 atgctgtaaa caacatggtc cttggtggcc atgttgttta cagccagtat aatatacagc
     9541 cttaacaaga tattccgtct ccggaatgga ctgttcacca ctgaataaca ttatgtctgt
     9601 tttatggatc tttgactgaa atttatcgca tatcttcatt tcattgtccg tctgaaaact
     9661 aaccttacaa gtaagcgtcg tctcagcacc gtctggcaga tcctgaaatt cctgagagga
     9721 tgatctgcgc ccaatatcct gagccggaac tttgctgcaa tgaaggccaa cgaaaaatgg
     9781 gggagtatgt tatagagtac ttgctacagg gtaaaaagag gtacctgtcg ctggttctcc
     9841 gttcagttta accgggagat tttctcctgc gcctgtcata aagtccggta atggagattg
     9901 ttaatacgat ggtgcacgat gcattgtcag tgcccggccc ggaggatgcc ccgtagctac
     9961 atacggatca gggttggcaa tatcgaatgg caggctatag gcaaagttaa agccccatgg
    10021 agtagcacaa aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tggagaactt
    10081 cttcggcaca ctgcaaaatc gcatcgtggt cgatcgctaa ttcgtcgtga atcactgaac
    10141 gatgttccat gcgaacagat attcgatcat atcgaaatat taaacattac ccctccccac
    10201 attgggcaaa aaaaaacaaa aaacgatcaa ttaacgatca attagtattg attcacataa
    10261 tctctcatca ttaatgagaa cattgtcaca tatttacaat atagaactgt tacttttagc
    10321 aagctgttag taaccaaccg ttactaataa ttttaattct taatactttg ttcattcaat
    10381 aaatgagttg agagaatata tgaataaaat atactccctt aaatatagtg ctgccactgg
    10441 cggactcatt gctgtttctg aattagcgaa aagagtttct ggtaaaacaa accgaaaact
    10501 tgtagcaaca atgttgtctc tggctgttgc aggtacagta aatgcagcaa atattgatat
    10561 atcaaatgta tgggcgagag actatcttga tcttgcacaa aataaaggta ttttccagcc
    10621 cggagcaaca gacgtaacaa tcactttaaa aaacggagat aaattctctt tccataatct
    10681 ctcaattccg gatttttctg gtgcagcagc gagtggcgca gctaccgcaa taggaggttc
    10741 ttatagtgtt actgttgcac ataacaaaaa gaaccctcag gccgcagaaa ctcaggttta
    10801 cgctcagtct tcttacaagg ttgttgacag aagaaattcc aatgattttg agattcagag
    10861 gttaaataaa tttgttgtgg aaacagtagg tgccaccccg gcagagacca accctacaac
    10921 atattctgat gcattagaac gctacggtat agtcacttct gacggttcaa aaaaaatcat
    10981 aggttttcgt gctggctctg gaggaacatc atttattaat ggtgaatcca aaatctcaac
    11041 aaattcagca tatagccatg atctgttaag tgctagtcta tttgaggtca cccaatggga
    11101 ctcatacggc atgatgattt ataaaaatga taaaacattt cgtaatcttg aaatattcgg
    11161 agacagcggc tctggagcat acttatatga taacaaacta gaaaaatggg tattagtcgg
    11221 aacaacccat ggtattgcca gcgttaatgg tgaccaactg acatggataa caaaatacaa
    11281 tgataaactg gttagtgagt taaaagatac ctatagtcat aaaataaatc tgaatggcaa
    11341 taatgtaacc attaaaaaca cagatataac attacaccaa aacaatgcag ataccactgg
    11401 tactcaagaa aaaataacta aagacaaaga tattgtgttc acaaatgggg gaaatgttct
    11461 gtttaaggat aatttggatt ttggtagcgg tggtattatc tttgacgaag gccatgaata
    11521 taacataaac ggtcagggat ttacatttaa aggagcagga attgatatcg gaaaagaaag
    11581 cattgtaaac tggaatgcat tgtattccag tgatgatgtt ttacacaaaa taggccctgg
    11641 tactctgaat gttcaaaaaa aacagggggc aaatataaag ataggtgaag gaaatgttat
    11701 tcttaatgaa gaaggaacat ttaacaatat ataccttgca agcggaaatg gtaaggtaat
    11761 actaaataaa gataattccc ttggcaatga tcaatatgcg gggatatttt ttactaaacg
    11821 tggtggcacg ctagatttaa atggacacaa tcagactttt actagaattg ccgccactga
    11881 cgatggaaca acaataacta actcagatac aacgaaagaa gccgttctgg caatcaataa
    11941 cgaagactcc tacatatatc atgggaacat aaatggcaat ataaaactaa cgcacaatat
    12001 taattctcag gataagaaaa ctaatgcaaa attaattctg gatggtagtg tcaacacaaa
    12061 aaatgatgtt gaagtcagta atgccagtct taccatgcaa ggccatgcaa cagagcatgc
    12121 aatattcaga agcacagcga atcattgctc cctggtattt ctttgtggaa cggactgggt
    12181 caccgttttg aaagaaacag agagttcata taataaaaag ttcaattctg attacaaaag
    12241 taataatcag cagacctcat ttgatcagcc tgactggaaa accggggtgt ttaaatttga
    12301 tacattacac ctgaacaatg ctgacttttc aatatcacgc aatgccaatg ttgaaggaaa
    12361 tatatcagca aataaatcag ctatcacaat cggcgataaa aatgcttaca ttgataatct
    12421 tgcagggaaa aatattacta ataatggttt tgacttcaaa caaactatca gtactaatct
    12481 atccatagga gaaactaaat ttacaggtgg catcactgca cataacagcc aaatagccat
    12541 aggtgatcaa gctgtagtta cacttaatgg tgcaaccttt ctgaataata ctcctataag
    12601 tatagataaa ggagcaaaag ttatagcaca aaattccatg ttcacaacaa aaggtattga
    12661 tatctccggt gaactgacta tgatgggaat ccctgaacag aatagtaaaa ctgtaacgcc
    12721 gggtctccac tacgctgctg atggattcag gctgagtggt ggaaatgcaa atttcattgc
    12781 cagaaatatg gcatctgtca ccggaaatat ttatgctgat gatgcagcaa ccattactct
    12841 gggacagcct gaaactgaaa caccgactat atcgtctgct tatcaggcat gggcagagac
    12901 tcttttgtat ggctttgata ccgcctatcg aggcgcaata acagccccca aagctacagt
    12961 tagcatgaat aatgcgatct ggcatctaaa tagccagtca tcaattaatc gtctagaaac
    13021 aaaagacagt atggtgcgtt ttactggtga caatgggaag tttacaaccc ttacagtgga
    13081 caaccttact atagatgaca gtgcatttgt gctgcgtgca aatctggccc aagcagatca
    13141 gcttgttgtc aataaatcgt tgtctggtaa aaacaacctt ctgttagtcg acttcattga
    13201 gaaaaatgga aacagcaacg gactgaatat cgatctggtc agcgcaccaa aaggaactgc
    13261 agtagatgtc tttaaagcta cgactcggag tattggcttc agtgatgtaa caccggttat
    13321 cgagcaaaag aacgatacag acaaagcaac atggactctg atcggctata aatctgtggc
    13381 caacgccgat gcggctaaaa aggcaacatt actgatgtca ggcggctata aagccttcct
    13441 tgctgaggtc aacaacctta acaaacgtat gggtgatctg cgtgacatta acggtgagtc
    13501 cggtgcatgg gcccgaatca tgagcggaac cgggtctgcc ggcggtggat tcagtgacaa
    13561 ctacacccac gttcaggtcg gtgcggataa caaacatgaa ctcgatggcc ttgacctctt
    13621 caccggggtg accatgacct ataccgacag ccatgcaggc agtgatgcct tcagtggtga
    13681 aacgaagtct gtgggtgccg gtctctatgc ctctgccatg tttgagtccg gagcatatat
    13741 cgacctcatc ggtaagtacg ttcaccatga caacgagtat accgcaactt tcgccggcct
    13801 tggcaccaga gactacagct cccactcctg gtatgccggt gcggaagtcg gttaccgtta
    13861 ccatgtaact gactctgcat ggattgagcc gcaggcggaa cttgtttacg gtgctgtatc
    13921 cgggaaacag ttctcctgga aggaccaggg aatgaacctc accatgaagg ataaggactt
    13981 taatccgctg attgggcgta ccggtgttga tgtgggtaaa tccttctccg gtaaggactg
    14041 gaaagtcaca gcccgcgccg gccttggcta ccagtttgac ctgtttgcca acggtgaaac
    14101 cgtactgcgt gatgcgtccg gtgagaaacg tatcaaaggt gaaaaagacg gtcgtatgct
    14161 catgaatgtt ggtctcaatg ccgaaattcg cgataatctt cgcttcggtc ttgagtttga
    14221 gaaatcggca tttggtaaat acaacgtgga taacgcgagc aacgccaact tccgttactc
    14281 tttctgataa cagcccgggc cgcgtttgcg gcccttcttc taccggagag aatatgtatt
    14341 accctgtgac agactatatc gctcttgctc tcattattag ctttcttttt ctgacattat
    14401 ttatctgcct gttatgcctt aaacatgagc gaataaaaaa agaaactatc aggcaaaaaa
    14461 atgcacatat tctggatcat ggctggaatg caactgagtt ctcatggttc cgatacggac
    14521 agtataacga aacgggtatt tacatctcga tcgagaagac cattatcatt acaatcaccg
    14581 tttctggtgg gt
//
LOCUS       DADKXM010000100        14272 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.101, whole genome shotgun sequence.
ACCESSION   DADKXM010000100 DADKXM010000000
VERSION     DADKXM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.101"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            212..1503
                     /gene="rstB"
                     /locus_tag="JQR05_002887"
                     /pseudo
     CDS             212..1503
                     /gene="rstB"
                     /locus_tag="JQR05_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
     gene            1579..2508
                     /gene="tus"
                     /locus_tag="JQR05_002888"
     CDS             1579..2508
                     /gene="tus"
                     /locus_tag="JQR05_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY5325005.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2505..3908)
                     /gene="fumC"
                     /locus_tag="JQR05_002889"
     CDS             complement(2505..3908)
                     /gene="fumC"
                     /locus_tag="JQR05_002889"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY5325006.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(4051..5697)
                     /locus_tag="JQR05_002890"
     CDS             complement(4051..5697)
                     /locus_tag="JQR05_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY5325007.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5896..7071
                     /gene="manA"
                     /locus_tag="JQR05_002891"
     CDS             5896..7071
                     /gene="manA"
                     /locus_tag="JQR05_002891"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY5325008.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7172..8680
                     /locus_tag="JQR05_002892"
     CDS             7172..8680
                     /locus_tag="JQR05_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY5325009.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8906..10171)
                     /gene="uidC"
                     /locus_tag="JQR05_002893"
                     /pseudo
     CDS             complement(8906..10171)
                     /gene="uidC"
                     /locus_tag="JQR05_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(10210..10377)
                     /locus_tag="JQR05_002894"
     CDS             complement(10210..10377)
                     /locus_tag="JQR05_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY5325010.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10393..11389)
                     /gene="uidB"
                     /locus_tag="JQR05_002895"
                     /pseudo
     CDS             complement(10393..11389)
                     /gene="uidB"
                     /locus_tag="JQR05_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11386..13197)
                     /gene="uidA"
                     /locus_tag="JQR05_002896"
     CDS             complement(11386..13197)
                     /gene="uidA"
                     /locus_tag="JQR05_002896"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY5325011.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVVLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13585..14175)
                     /locus_tag="JQR05_002897"
     CDS             complement(13585..14175)
                     /locus_tag="JQR05_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5325012.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
BASE COUNT         3592 a         3700 c         3388 g         3592 t
ORIGIN      
        1 actaaagaac aacaaagcgt cacgttaaat taaaccttag cataataaac aagccccggt
       61 ggaaaattga atttactgaa tatgaatacg ccggggatac atccgtctgt tccggacatt
      121 atcttccaaa caaggtctta tccagttggc ggtttacatt gctccacagt cgtgaattaa
      181 tacgaatagt attctgttgc gggcgatgcc aatgaaaaaa ttgtttatcc agttttacct
      241 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
      301 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcgc tgtatctgat
      361 gcgcagcgaa ttgcgagaga tccccccaca cgactggggt aagacgctga aagagatgga
      421 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
      481 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
      541 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
      601 cctccatcaa atgcgattgc tggatatcgc cctgatcgct tttattgcta tttccctcgc
      661 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
      721 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
      781 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttaattgc
      841 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
      901 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
      961 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
     1021 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
     1081 ggcagatatt caggcagtaa cgcccgataa aacggtacgg ataaaaacgc tcatgcaagg
     1141 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
     1201 cgccctgcgc tactgccatt caacggttga aaccagcctg ctactagagc gacattaatt
     1261 gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt tgaacctttt
     1321 gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg gctggcaatt
     1381 gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag cgaactgggt
     1441 ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt tacctctgcc
     1501 tgacactacg cgcacgatgg tcaagtcacc acgactgtgt tataaaataa gtatgttgta
     1561 actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa cactaccttt
     1621 cgccagatgg aacaagagct ggctacattt gccgctcatc ttgagcaaca caagctattg
     1681 gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa tccgcttaat
     1741 cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt gcgtcatttc
     1801 cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc tgtccgtctg
     1861 cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt cagtcatatt
     1921 cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga atcagaactc
     1981 cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat cacccttaat
     2041 gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg ttgggctaat
     2101 aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg
     2161 aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa actggagcga
     2221 gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa acgtccagtg
     2281 aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt ccaacacgcc
     2341 tgccctacac cactgattgc actgattaat cgggataatg gcgcgggcgt gccggacgtt
     2401 ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc tcaggcgcag
     2461 ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cagattaacg cccggctttc
     2521 atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc gctaagatac
     2581 cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt tttggcgatc
     2641 tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat cagcgattca
     2701 ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca gtgtttgtta
     2761 aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattatg gatcaccatc
     2821 ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat cgccacgtcg
     2881 ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg gttcactttc
     2941 cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc aattccgcag
     3001 cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga cgcagccaac
     3061 cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc ttcaaatttg
     3121 ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc tacgcgacgc
     3181 gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccaag agccagttcc
     3241 gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat cgctacccag
     3301 ccggaaatct cctgccctag tgttagcggc gtggcgtcct gcaagtgggt tcgaccgatt
     3361 ttgacgatat cggcaaatgc acgcgatttt tcactcagcg tctgtgtcag ggttttaagc
     3421 tgcggaatga gttgcttgcg cagtgccagc agcgccgcaa cgtgcatcgc cgtcggaaag
     3481 acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt acgttccatc
     3541 ccgcgcacac cgccgagtaa ttcactggcc cggttagcca gcacttcgtt catgttcatg
     3601 ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc gtcatgctgt
     3661 cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc agacaacaag
     3721 tctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc atgaatcagt
     3781 gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg
     3841 ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc gctgcgtact
     3901 gtattcatgg cctgctcctc acctgattaa ttttttcttt ttgttttgct ttcgttaagc
     3961 aacttttagc tcactcatta tttactattt gataacaaat gtttggtctt tcgtgccatg
     4021 taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc actgtgtgag
     4081 ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg ccgggaaatc
     4141 ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa cacattccag
     4201 gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc caaggtagaa
     4261 gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt tcgccagcat
     4321 gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc gtccggcggt
     4381 cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac ccgcgtagta
     4441 aatcggatga tctttaatgt attgcggcag cccttcaccg ttatccatcc gctctttcag
     4501 tttggcgtga gcaatatcac gaccaacgat aatcgtgccg ttaagcgata agcgtgtaga
     4561 aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg
     4621 caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac ctggattatg
     4681 ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat tacggtcagc
     4741 agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca ggcgaatcac
     4801 gcggatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga gattttgcgc
     4861 ttcgatcagt aattcttttt ccagttccac atcgcggaac gcctgaccgt gctcattccc
     4921 ttccgttggc agttcatcat agtatttcgc ggaagccagt ttcaccgttt taaggttcgt
     4981 ttctgcagaa gttccaccaa taacgaacgc aatatgatac ggaggacagg ccgcggtacc
     5041 cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtca gtaacgcttt
     5101 ggtttcctga tagagatacg tcttgtttgc cgaaccacca ccttttgcga tacagaggaa
     5161 tttgtattcg tcgccatcaa cggcataaag atcgatctgc gctggcagat tggtgccggt
     5221 attcacttct ttatacatat ccagcggcgc gttttgcgag tagcgcagat tatcttcgat
     5281 ataagtgtta tagacaccgc gcgccagcgc cgcttcatca ccaccaccgg tccatacacg
     5341 ctgccctttt ttaccaacaa taatcgcggt gccggtatcc tgacaggttg gcagaacgcc
     5401 tttcgccgcg atgtcggagt tacgcaggaa ttgcagcgcc acatatttat cattttcgct
     5461 ggcctccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac gcagcataaa
     5521 cgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg cgactttcaa
     5581 aatctcctgc ccttcaaatt cagatacgct aacgtgttca ctggttagca ggtaatactc
     5641 agtatcatct tttttgagtg gaaaaggaga ctgataatga aagggtttgt ttgacattgt
     5701 tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg ccctgttaaa
     5761 agcgagtaac aatatcctac acagtttttt aacaaaaact gagactagta cgactttttg
     5821 cggctccagg ttacttcccg taggattctt gctttaatag cgggattaat ttccacatta
     5881 aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc ctggggtagc
     5941 aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc gatggccgag
     6001 ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc cggagatatc
     6061 gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga ggccgttgcc
     6121 aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca gccactctcc
     6181 attcaggttc atccaaacaa acacaattct gaaatcggtt ttgccaaaga aaatgccgca
     6241 ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa gccggagctg
     6301 gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgaattttc cgagattgtc
     6361 tccctactcc agccggtcgc cggtgcacat ccggcgattg ctcacttttt acaacagcca
     6421 catgccgaac gtttaagcga actgttcgcc aacctgttga atatgcaggg tgaagaaaaa
     6481 tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga accgtggcaa
     6541 acgattcgtt taatttctga attttacccg gaagacagcg gcctgttctc cccgctgtta
     6601 ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac
     6661 gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt gctgcgtgca
     6721 ggtctgacgc ctaaatacat tgatattccg gaactggttg ccaatgtgaa attcgaagcc
     6781 aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact ggacttcccg
     6841 attcctgtgg atgattttgc cttctcgctg catgacctta gtgataaaga aaccaccatc
     6901 agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt gtggaaaggt
     6961 tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa cgaatcaccg
     7021 gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta agagcttact
     7081 gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc aggcggccag
     7141 tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag cggtaggcgt
     7201 cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca agaagattga
     7261 aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga cagctcctga
     7321 atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca gccagttgca
     7381 actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg gtcagagcgt
     7441 catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta aaaaactgaa
     7501 cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag taagcaaacc
     7561 actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca ttggttacag
     7621 cggtgattcc agttccgata tttcgctcaa tccactgaat tacgagcaaa aggatgaaaa
     7681 agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca aagccatctc
     7741 cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca accagaaagt
     7801 gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa gttttggtga
     7861 gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg aaggcaaaga
     7921 actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca atgacggtaa
     7981 aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga atcaggatct
     8041 gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct ggcatcagtt
     8101 tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa ttgccaacaa
     8161 tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc cgctgatgtt
     8221 gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc agggtgaaag
     8281 tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag cgccgcaaac
     8341 gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga ccattccggt
     8401 ggatatggca actgagttga tgactcaggt agcgaagctg gaaggttatc aggaagatca
     8461 agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc agatgttccg
     8521 tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatgcta acggtcagat
     8581 aacgttaaat gggcagaaaa tgtcgttaga agattttgtt ggcatgtttg cgatgccagc
     8641 tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca aaacgccgga
     8701 gaattttcac tccggcgttt tcatctgaaa acaaaccaga gattgcctga tgcgctacgc
     8761 ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta ggctggataa
     8821 agcgtttacg ccgcatccgg cataaaaaac gcgcactttg tcaacaatct gaaacgccgg
     8881 aggtttttct ctccggcgtt atttcttact tcagcataaa gtcatactga atactcattt
     8941 tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt
     9001 tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca
     9061 taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat tcatagccgc
     9121 gagtcacaat cggtgcaaat gtcagcgtaa attgcggagt tacacggtag ttgacaccta
     9181 attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa
     9241 tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata
     9301 cgcttcccca cggcgcattc taggtcaatt gtccactaac caacgccgtt tcattgggct
     9361 ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca
     9421 acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa
     9481 catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg
     9541 ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt
     9601 catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc
     9661 acagttcaat atttggcgtt aaagcccaat aacctaatgc tccttcagaa actgtcggta
     9721 atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat
     9781 aatccgtccc gaagcgacct aatttaagca ggctattttc actgggtttt atttttactg
     9841 cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag
     9901 cacgagcacc taatttataa acataatacg ctccgccttc aacgccaata atattggaat
     9961 aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg
    10021 cgccagcact gggcttatca gccctgcgca attcgttttt cagtctgatg cgtagtcccg
    10081 cttcatcgtc agccagttgc gccgcgtaag cagaggtaag gccagaggca gccgtcaggc
    10141 aaataacggc catggccact atttttctca tagttgcacc tttgatgttc tgattatttt
    10201 tattgaatat taattagtga tatcgctgat taattgctgc tgcacttttt tacgattatc
    10261 aatttcaacc acgatttctt tgaatttttt atccgtgagc ggataaaacc agataataac
    10321 gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat
    10381 aacttccggc gtttacacct gattggcgat atatccgctt aatcccaaaa taaaggcagg
    10441 aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag
    10501 cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag cttccagtgc
    10561 ccacatcaca gtcatagtaa cgccctgacc aagtgaacag cccggtatca tttaacacat
    10621 agcgcacgta gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg
    10681 caccgatgca caacataaac agcgggcgat tccgtttcag ggtttgcaaa ctgatcttca
    10741 atgacggctg cgccacgata cgtaccacat tttcacgctt cgatttgaag cagatgaagt
    10801 aaagcaccat tccggcaatc gccagcacaa tcgtccagaa atggtatacc gacaccatct
    10861 cttccgggct ggagttctta atgctcggtc ctatcagaaa tgccaggcat acaaaggtca
    10921 atgaagcggc aatccaacga gccgcgccca gacgggcgcg ggattgtggt tgttgggtca
    10981 tcgcggtagc aagtgaacca taaggaatat tcaccaggct gtagcaaagc ccgaggccca
    11041 tgtaggtcaa atatgcatac accactttgc taccatggct ccagtcggtc ggtacccaga
    11101 ataccagcac gctgaagatc attaacggcg cagtaccgaa gagtaaaaac gggcggaatt
    11161 ttccccagcg ggtattcaca ctgtccacca ctcgtccggc aaagacgtcg gcgaaggcat
    11221 cgaatacccg caccagtaac agcatggtgc ctgccgcagc ggcaccgacg ccagcgacgt
    11281 cggtgtagta actcaatagg aagagcgccc ccattgcgaa ggcgaagtta ttggcgacgt
    11341 caccgaggct gtagccgacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc
    11401 ctgctgcggt ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc
    11461 cgacttcggt ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat
    11521 gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac
    11581 gcgatcaaag acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat
    11641 gtcggtgtac attgagtgca ggccggctaa cgtatccacg ccgtattcgg tgatgataat
    11701 cggctgatgc agtttctcct gccaggccag cagttctttt tccagtacct tctctgccgt
    11761 ttccaaatcg ccgctttgga cataccaacc gtaataacgg ttcaggcaca gcacatcaaa
    11821 gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg
    11881 acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc
    11941 ttgcggacgg gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt
    12001 gtcacgcgct atcagctctt taatcgcctg taagtgcgcc tgctgagttt ccccgttgac
    12061 agcctcttcg ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga
    12121 gaggttaaag ccgacagctg cagtttcatc aatcaccacg atgccatgtt catctgccca
    12181 gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca
    12241 gtccattaat gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc ctcgcaaatc
    12301 cgcatcttca tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg
    12361 ttctcccttc actgccactg accggatgcc gacgcgcagc gggtagatat cacactctgt
    12421 ctggcttttg gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg
    12481 cggattcacc acttgcaaag tcccgctggt gccttgtcca gttgcaacca cctgttgatc
    12541 cgcatcacgc agttcaacgc tgatatcacc attggccacc acctgccagt caacagacgc
    12601 gtggttacag tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg
    12661 cgtggtgtag agcactacgc tgcgatggat cccggcatag ttaaagaaat catggaagta
    12721 agactgcttt ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca
    12781 gttcagttcg ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg
    12841 cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt
    12901 gacccacact ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc
    12961 ccaacctttc ggtataaaga cttcgcgctg ataccagacg ttgcccacat aattacgaat
    13021 atctgcatcg gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg
    13081 taacgcgctt tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc
    13141 ccacaggccg tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag
    13201 ggactcctca ttaagataaa aatactggtc aacctttaat ctgattagtt aaccaattct
    13261 gtgttgcaca cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg
    13321 acaccgcgat caaaaaacca aaaactgcaa tacccacagc gtgatatgga tcgcattaat
    13381 ctttaaaaca gcgttgacag aagccataaa ataagcaatc gaacgttcgt tcgagagtat
    13441 tgggaacgat aaactctcat gacatatggc tacggcgaat attttggggg ggaattctcc
    13501 gaacacgggg aggataattt ttgcacagag aaattacgat atgaaacgcg ggcacagtta
    13561 tcaccagccc gcgaaatgag aggatcagga tgcggttaat ataccgccaa tcatcgcgcg
    13621 taatccctga gcaaacgacg cttcacgcgc caggtcttcc gcttcgatat cggccagtac
    13681 accgtaggtc agatccagca gtaaacgtga agtcattgct gtgttgatgt ctgggcttat
    13741 ctcgcctttt tgctgggcat cacgcatccg ctgggcaaca aattcagtga tcgtcatatg
    13801 cttattttta agcatggcgg cgacctgtgg gttacgcatc ccttccgcca taatttcgac
    13861 caccagcgcc cgttgcccaa aggcttcatg ggtgagagag acaatagact cgaccatata
    13921 gtcaacaaaa tgaatccctt caatcggttc ccggaaacga gccagcgccc tctcctggtc
    13981 ctgtaagata atcgcctgaa tcaaggcttc tttggagatg aaatggtgat agagcgtccc
    14041 aggactaatg gcgcaagatt tacagatggc tttcatcgag gcactgtgaa atccattttc
    14101 tgaaaaaatc tctctggcag cattgaggat ccgggtccgt gtcggttgtg cttcagtctg
    14161 catgttatcc atcattaaac cattatagtt agcgtgaata actagtttac cgcgttgtca
    14221 ggtgagcgcc tatcattgct gttgaatagt gcgaaggtac actctattcg ta
//
LOCUS       DADKXM010000101        14141 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.102, whole genome shotgun sequence.
ACCESSION   DADKXM010000101 DADKXM010000000
VERSION     DADKXM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.102"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..512)
                     /locus_tag="JQR05_002898"
     CDS             complement(<1..512)
                     /locus_tag="JQR05_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY5325013.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..1210)
                     /locus_tag="JQR05_002899"
     CDS             complement(758..1210)
                     /locus_tag="JQR05_002899"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5325014.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(1220..1618)
                     /gene="yafO"
                     /locus_tag="JQR05_002900"
     CDS             complement(1220..1618)
                     /gene="yafO"
                     /locus_tag="JQR05_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY5325015.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(1621..1914)
                     /gene="yafN"
                     /locus_tag="JQR05_002901"
     CDS             complement(1621..1914)
                     /gene="yafN"
                     /locus_tag="JQR05_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY5325016.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(1966..3021)
                     /gene="dinB"
                     /locus_tag="JQR05_002902"
     CDS             complement(1966..3021)
                     /gene="dinB"
                     /locus_tag="JQR05_002902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY5325017.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCTHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(3092..3877)
                     /gene="lafU"
                     /locus_tag="JQR05_002903"
     CDS             complement(3092..3877)
                     /gene="lafU"
                     /locus_tag="JQR05_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY5325018.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQRLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            3822..5561
                     /gene="flhA"
                     /locus_tag="JQR05_002904"
     CDS             3822..5561
                     /gene="flhA"
                     /locus_tag="JQR05_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY5325019.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNYPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            complement(5778..6275)
                     /gene="rayT"
                     /locus_tag="JQR05_002905"
     CDS             complement(5778..6275)
                     /gene="rayT"
                     /locus_tag="JQR05_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY5325020.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(6451..7064)
                     /locus_tag="JQR05_002906"
                     /pseudo
     CDS             complement(6451..7064)
                     /locus_tag="JQR05_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            7356..8096
                     /locus_tag="JQR05_002907"
     CDS             7356..8096
                     /locus_tag="JQR05_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY5325021.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(8067..8834)
                     /locus_tag="JQR05_002908"
     CDS             complement(8067..8834)
                     /locus_tag="JQR05_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY5325022.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(9040..9618)
                     /gene="lpcA"
                     /locus_tag="JQR05_002909"
     CDS             complement(9040..9618)
                     /gene="lpcA"
                     /locus_tag="JQR05_002909"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY5325023.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9858..12302
                     /locus_tag="JQR05_002910"
     CDS             9858..12302
                     /locus_tag="JQR05_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY5325024.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(12345..12818)
                     /gene="ivy"
                     /locus_tag="JQR05_002911"
     CDS             complement(12345..12818)
                     /gene="ivy"
                     /locus_tag="JQR05_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY5325025.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12972..13742
                     /gene="yafV"
                     /locus_tag="JQR05_002912"
     CDS             12972..13742
                     /gene="yafV"
                     /locus_tag="JQR05_002912"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY5325026.1"
                     /translation="MPGLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            13852..14084
                     /locus_tag="JQR05_002913"
                     /pseudo
     CDS             13852..14084
                     /locus_tag="JQR05_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         3401 a         3613 c         3715 g         3412 t
ORIGIN      
        1 gagccgctat gaaccagcag ttgcagatgc tgcgcatcca gacctgccag tgcaaacagt
       61 tcagcgttga tgatttgatc aacttgttgc agttcagcga aatgattgcc accaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cacagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gcctgcggag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccggggtgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat ataattgccc atataccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gacttgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atctagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttataacgca
      781 tataaaaatt aataaaccac gctccccggc attcaacgcg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgcttgc gtccaccgta agttcggatt
      901 cagacttaat caaaggtttt aacaaagcgc tggcaacccc acggcgggtg tattcagggt
      961 caacaaataa catatcgata tagtgttcaa cgcaggtaat aaagccgacc agttttgcat
     1021 taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actgacggtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgataatct cttatttgta
     1201 tgttattcat aatataaatt caaaaacgca tgcgaaacgc ttctgccatt tttccaattt
     1261 tatgcatttg gttgttatct cgagccagtt tatgaggttc aggcttcaga atggcaatga
     1321 gcaaccatgc ttgctcatca aacgcaccct gacaatatac caaatgcgct tcgtcattcg
     1381 ttctgctgaa ttggcgcaac tgtggcggaa atggattatt cacatttgcc agatgaatat
     1441 gagcaactcg ctcaaatttg attaatggcc aggtaaacga gtcgtcgtag agtgcatcgc
     1501 gaccaaatat atctggcaaa acaccgtcac gcttatagga aataaaatcc gccgttaacg
     1561 catcaagttc ctctgctgta agttgcaggc gaataagttt tgttttgaat acccgcatcc
     1621 ttattcctta aagtcattga aatcatcatc cgtcatatca ttaagatatt gtttagcgcg
     1681 gcgtgtaatt tcctctaatc ttgcagcact tggacgaaag cgcgccttta tgggcttttt
     1741 ctcctcttgt tcagcaagca tgtccattaa cgcttcgaat gcgctggcac ttaagagata
     1801 tcctgcgggg cgattattag aaagaaccgc aactggttga tcaataaagt atttagctgg
     1861 gtttttacgt aactcagtga tattgaccga tttttcagcg agaattcgat gcatacagtg
     1921 ataccctcat aataatgcac accagaatat acataatagt atacatcata atcccagcac
     1981 cagttgtctt tccatttgcg ggtcaagcaa cgtcacatgc agccccacca gacgcacacc
     2041 gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattaagt cagctttatt
     2101 cagccgcggc cagacgtgct cctgggtggt ctgctgaaaa tcgtcgaact ttaatttcac
     2161 cccctggcga gcaatcagta aatcaggttt tacctttgcc agacggcgtt caagttccgg
     2221 atacagccgc tcgataatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt
     2281 gcgttccacg ccgacggatt ttcgtaaccg ttcgctgtta acgtcgcgct cgtcaatccc
     2341 ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gaatcaccag
     2401 atcacacttt tgtacatcac cgcaggtccg cagccccatc gcttccagtt ttgccgccga
     2461 gactttgccg acgccgggga ttttttccag tggtaaggtt tgtaaaaatg ccggaacttc
     2521 tgccggcgta atcacaaact gaccgttggg tttattcatg tcggaggcga ttttggcgag
     2581 aaactttact ggtgccacgc ccgcagatgc cgtcagttgc agctcgttga agatcgtctg
     2641 gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat cggtgacatc
     2701 gagataagcc tcatccagtg acaacggttc aatgcgcgag gtgtagcgcg agaagatttc
     2761 acggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg
     2821 tgtgcataat ttgagcgcca tcccagtagg catagcgcta cgtacgccaa atttacgcgc
     2881 gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg
     2941 gatatcgcgc agggcgggat tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc
     3001 cacatgaatg attttacgca ttgctcacct ctcaacactg gtaaagtata cagtgatttc
     3061 aggttttgag aaatgcgtaa agattcagca tttagcgcat tcgctgcgaa agcacctgct
     3121 gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt
     3181 gatacagcgt atcggacgca cttttggtca gcaccataat ctcaatacgc cgattgcccg
     3241 cgctttgcgg attcttcgcg tccagcagca tctggtccgc cattgcgctt acctgcatca
     3301 ctttatcttc cggcattccg gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc
     3361 ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg
     3421 taataataat tttgttatcg agcgagtcga acactggcgc gagctccacc agcaaagttt
     3481 taaagaacgg cataatctgc gcgctgccgc gttcgaacat attgcgattc tggtcgtctt
     3541 taatcagcac gcgtaatctc tggggaacaa tctccatctc cagattcgct tccatatgcg
     3601 catcacgggc aatggtgttg atactggttg ccaactcgcc aagctcggtg gcggatttct
     3661 tcttcagcag cgccgttttc tcattcacat cacgcgcttt tttctccgtc tcttccggca
     3721 ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacagcc
     3781 caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt
     3841 tactgacgct gttgacaatc aactttatcg tcgtcaccaa agaggccgag cgtatttccg
     3901 aggtttctgc ccgctttacc ctggacgcga tgcccggcaa acagatggcg attgacgccg
     3961 atcttaacgc cggattgatc aaccaggcgc aggcgcaaac ccggcgtaaa gatgttgcca
     4021 gcgaggccga tttctacggc gcgatggacg gggcatcgaa gtttgtgcgc ggagacgcca
     4081 tcgccgggat gatgattctg gcgatcaacc tgagcggcgg cgtctgtatc ggcatcttca
     4141 aatacaacct gagcgccgac gccgccttcc agcaatacgt gctgatgacc atcggcgacg
     4201 gcctggtggc gcagatccct tccctgctgc tctccaccgc ggcggcgatt atcgtcaccc
     4261 gcgtcagcga caacggcgat atcgcccatg acgtgcgcca ccagttgctg gcaagcccgt
     4321 cggtgctcta caccgccacc gggatcatgt tcgtgctggc ggtggtgccg gggatgccgc
     4381 atctgccgtt tttgctgttc agcgccctgc ttggctttac cggctggcgg atgagcaaac
     4441 gcccgcaggc ggcggaggcg gaagagaaaa gcctcgaaac gctgacccgc accatcactg
     4501 aaaccagcga acaacaggtc agttgggaaa ctattccgct gatcgagccc atcagcttaa
     4561 gcctcggcta caagctggtg gcgctggtgg acaaagcgca gggcaacccg ctcacccagc
     4621 ggattcgcgg cgttcggcag gtgatttccg acggtaacgg cgtgctgctg ccggagatcc
     4681 gcattcggga aaacttccgc ctcaagccca gccagtacgc tattttcatc aacggcatta
     4741 aggctgatga agcggatatt ccggcggata aactgatggc gctgccctcc agcgaaacct
     4801 acggcgagat tgacggcgtg ctggggaact acccggcgta cgggatgccg gtcacctgga
     4861 tccagcctgc gcagaaagcg aaggctctga atatggggta tcaggtgatc gacagcgcca
     4921 gtgtgattgc cacccatgtg aacaagattg tgcgcagcta tattcctgat ttgtttaact
     4981 atgatgacat cacgcagttg cataaccgtt tgtcgtcgat ggcaccgcgc ctggcggagg
     5041 atttaagcgc ggcgctcaat tacagccaat tgctgaaagt gtaccgggcg ctgctgaccg
     5101 aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt gctggtcgcc agtagcacgg
     5161 tgactaaaga tcatattctg ctggcggccg atgtgcgcct ggcgctgcgg cgcagcatta
     5221 ctcatccgtt cgttcgcaag caggagctga cggtgtatac gctgaataat gagctggaaa
     5281 atctgctgac caacgtagtg aatcaggcgc aacaggccgg caaagtgatg ctcgacagcg
     5341 tgccggttga cccgaatatg ctcaaccagt tccagagcac gatgccacag gtgaaagagc
     5401 agatgaaagc ggcggggaaa gatccggtgc tgctggtgcc gccgcagcta cgccctttgc
     5461 tggcgcgtta tgcaaggttg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc
     5521 cggatgagct ggagttgaag attatggggg cgttgatgta agaatccggc atcggtgcac
     5581 aattgccgga tgcggcgtaa acgccttatc cggcctaccg ttcaggcaca agtttgtagg
     5641 catgataaga cgcgccagcg tcgcatcagg catctgcgca taactgccgg atgcggcgtg
     5701 aacgccttat ccggcctacc gttaaagcac gatttgtagg ccggataagc gtaagcgcat
     5761 ccggcaccag cgcacagtta taaaatgata cgctccccgg cacttaaatc cgttatgtct
     5821 cccgcccaat cgatgggata taaccctcgc gcgacatcgc gatggaacgt tgagaatggc
     5881 caatcactca cctgctttac ccagccatgt ttctctggat ttatataaat ataatcaaca
     5941 tgatggcgat aatcttttgt attgcgaatg gcatgctccc aaaaacgcgg ttgccagata
     6001 tttttcaatc cgcaagcatg ggtgaactgc tttttaatct cccgccagcg agaggaaaaa
     6061 tcatcatcgc tttcgggtaa tgtccatatg caatgcatat gctccggcaa aacgacccag
     6121 gcgttaattt caaaaggtct gtctcgctta acgttaataa tggcgttacg gagcgtctgg
     6181 aactgggtgg tcagaagatg gcttcgacga ttgcgtaaat ttaccgtgaa aaaccatgtt
     6241 ccccccttga tgtaataacg acgatattcc gacatagcga ctcctttttc tcaacggtaa
     6301 gaggaaaagg aaaggccgac aatgacgctg tgttatatcg ggaaagcggc tcgcaaaaac
     6361 gcacagattt gtaggtatga taagacgcgt cagcgtcgca tcaggcatcc gtgcacaacc
     6421 gccggatgcg gcgtaaacgc cttatccggc ctacaaaatc atctcttccg tcaaaatcct
     6481 gcgcgcgcct aaaaaatggt cctgccagaa aggttcggct aaccggctta cccgaatggt
     6541 ttccccggtg cgcagtgact caataaactg accgtcgccc agatacaccc ccatatggtc
     6601 ggcaatctcg cggctgtgga tatggaaaaa tagcaaatct ccccggcgca ggtcgttatt
     6661 cgccacaatc gttgcccgac ggtagtggta catctcattg gccgtgcgcg ggagtttcgc
     6721 ctcaaggatc ttgttgtagg cataaaaaac taacccacta caatcgaagc cttgatcggg
     6781 ccgcgtgccg ccccagacgt aaggcttgcc aagctgctgc tccaggcggt gaatcgccac
     6841 ttcggtgatg ttatgcaggt gatcgctgct taaaaagtgg ttgttttccg ccagcgcctg
     6901 ccagcgtctg tcactcgcct tgagcggcgc aggaaaccat tctggatgct gttttatctg
     6961 ctcccggttg tggtggcgct ggcgagtaaa gggagcaaaa cgcataacga aaacacgcct
     7021 ggatgcaaaa atctgccgag caagaacgaa gacatgaaag acataaaccg gctgaatgaa
     7081 ggtgagaatt ttaaaagacc ggaggagtaa acgggattat taattgctta tttgcatatt
     7141 actattgaga tttcataatc ttaggaaata aatgatacat gcgcttttcg gactattgaa
     7201 aactgcccct gtgctaaatt tgtaaaccca gcgccgacag gcttgcgtaa aactcttttg
     7261 gtgataatgt gagccttcca aatccagact attccgattt gggatccatt ttgccgtcct
     7321 agcgtgtgta accgttttat caaggaataa gcagtatgcg taaaatcgca ttaattcttg
     7381 cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct gggcagcagt agctcgacca
     7441 cgccagtgag caaagagtat aagcagcagt tgatgggatc ccctgtctac atccagatct
     7501 tcaaggaaga acgtacgctc gatctttacg tcaaaatggg cgagcaatat caactgctcg
     7561 acagttataa aatctgtaaa tattccggcg gcttagggcc aaaacagcgt cagggcgatt
     7621 tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca gttaaaacca gacagccgtt
     7681 actacaaagc gattaatatt ggattcccca atgcttatga ccgtgcgcat ggttatgaag
     7741 ggaaatacct gatgatccac ggcgattgtg tatccatcgg ctgctacgca atgaccaatc
     7801 agggtattga tgagatcttc cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg
     7861 tgcaggtgag tatttaccca ttccgcatga ccgacgccaa tatgaagcgc cataaatatt
     7921 ccaactttaa ggacttctgg gagcagctga agccgggcta cgactacttt gagcaaaccc
     7981 gtaagccacc gaccgtttct gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc
     8041 acgaagtggt gcagccacaa ctggcatcaa actacacgct ccccgaggca aaataagcgc
     8101 cattcgcctg gcataatttt ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc
     8161 accgtgacca catcattcgg tgtggtctgc gagctgaagt cgatttccac atcctgatcc
     8221 agcaacgttg ccacgccaaa cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa
     8281 tacgccatta catagcgccc gtccgaaagc agcatgttga aaacgccctt cgcccgcagt
     8341 tcatccgcca gtgaggcgat atatttaaac actgccgcca tgttgcccgg cgtgcgcggg
     8401 taacgctgcg ttaatttatg cagcagccag caaaaggctt tttcgctgtc ggtttcaccg
     8461 accgggcgga agttgccggt ttccagtgat ttgtagcccg tcagttgtcc gttatgggca
     8521 taagtccagt tacgccccca taactcgcgg gtaaatgggt gagtattttc cagcgccacc
     8581 tcgccccgat tagcctggcg aatatgagcc accaccgaac aggattttat cgggtagtcc
     8641 tggacaagtt tggcgatggg ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag
     8701 cctttacctt cgtaaaaggt aatgccccag ccatctttat gtggcccggt tccaccacca
     8761 cgctgtacaa gtccggtgaa actaaagcag atatcggtag ggacgttggc gctcatcccg
     8821 agcaattcgc acatgggtaa cctccacaac agcgggagcg catggacccc ggcgaagatt
     8881 gatttaagaa ttttcaggtc ggatgcggcg taaacgaatt attcggccta cggttcgggt
     8941 acagatttgt aggcatgata agacgcgtca gcatcgcatc aggcatcagc gcacaagtgc
     9001 cggatgcggc gtaaacgcct tatccggcct acgccagact tacttaacca tctctttttc
     9061 aattaactgg atcaggatat ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata
     9121 accaaagtgc ggaacgcgaa tttcgatatc cgccgtgcca gccattttac cgccgtcttt
     9181 accggtcagg gtgatcactt tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac
     9241 gtttgcagag ttaccggagg tggagatccc cagcagtaca tcgccttcgc gacctaccgc
     9301 ttcaacgtag cgggagaaaa tatcattgaa accgaaatca ttaccgacgc aggaaatatg
     9361 actaacgtca gaaatagcaa tcgccgggta gcccggacgg ttttcacggt agcgaccggt
     9421 caactcttcg gcaaagtgca tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac
     9481 tttgccaccg gctttaaagc tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat
     9541 attggcgtca tcttttaaaa agttagccag cgtttccgcc gcttcgttca gttcgttacg
     9601 aataagatcc tggtacatga ggatatcctt cagcataaat gtaatagaca aaatgcagtg
     9661 taccggatac cgccaaaagc gagaagtacg ggcaggtgct atgaccagga ctttttgacc
     9721 tgaagtgcgg ataaaaacag caacaatgtg agctttgttg taattatatt gtaaacacat
     9781 tgctaaatgt ttttacatcc actacaacca tatcatcaca agtggtcaga cctcctacaa
     9841 gtaaggggct tttcgttatg atgattttga gtattctcgc tacggttgtc ctgctcggcg
     9901 cgttgttcta tcaccgcgtg agcttattta tcagcagtct gattttgctc gcctggacag
     9961 ccgccctcgg cgttgctggt ctgtggtcgg cgtgggtact ggtgcctctg accattatcc
    10021 tcgtgccatt taactttgcg cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg
    10081 gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca
    10141 ccacctggtg ggagggcgac ttgttccagg gcaagccgga ctggaaaaag ctgcataact
    10201 atccgcagcc gcgcctgacc gctgaagagc aagcgtttct cgacggcccg gtagaagaag
    10261 cctgccggat ggcgaatgat ttccagatta ctcatgagct ggcggatctg ccgccggagt
    10321 tgtgggcgta ccttaaagag catcgcttct tcgcgatgat tatcaaaaaa gagtacggcg
    10381 gactggagtt ctcagcttat gcccagtctc gtgtgctgca aaaactctct ggcgtgagcg
    10441 ggatcctggc gattaccgtc ggcgtgccaa actcattagg cccgggcgaa ctgttgcaac
    10501 attacggcac tgacgagcag aaagatcact atctgccgcg tctggcgcgt ggtcaggaga
    10561 tcccctgctt tgcactgacc agcccggaag cgggttccga tgcgggcgcg attccggaca
    10621 ccgggattgt ctgcatgggc gaatggcagg gccagcaggt gctggggatg cgtctgacct
    10681 ggaacaaacg ctacattacg ctggcaccga ttgcgaccgt gcttgggctg gcgtttaaac
    10741 tctccgaccc ggaaaaatta ctcggcggtg cagaagattt aggcattacc tgtgcgctga
    10801 tcccaaccac cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt
    10861 tccagaacgg accgacgcgc ggtaaagatg tcttcgtgcc gatcgattac atcatcggcg
    10921 ggccgaaaat ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg
    10981 gcatcaccct gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg
    11041 cgtatgctca cattcgccgt cagttcaaaa tctctatcgg taagatggaa gggattgaag
    11101 agccgctggc gcgtattgcc gggaacgctt atgtaatgga tgctgcggca tcgctgatta
    11161 cctacggcat tatgctcggc gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact
    11221 gtacccaccg cgggcagcag tcgattattg atgcgatgga tattaccggc ggtaaaggca
    11281 ttatgctcgg gcaaagcaac ttcctggcgc gtgcttacca gggcgcaccg attgccatca
    11341 ccgttgaagg ggctaacatt ctgacccgca gcatgatgat cttcggacaa ggagcgattc
    11401 gttgccatcc gtacgtgctg gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt
    11461 tcgataaact gttgttcaaa catatcggtc acgtcggtag caacaaagtt cgcagcttct
    11521 ggctgggcct gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc actaaacgct
    11581 actatcagca cctgaaccgc ctgagcgcca acctcgccct gctttctgat gtctcgatgg
    11641 cagtgctggg cggcagcctg aaacgtcgtg agcgtatctc ggcacgtctg ggggatattt
    11701 taagccagct ttacctcgcc tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg
    11761 aagccgatct gccgctggtg cactggggcg tacaggatgc gctgtatcag gcagaacagg
    11821 cgatggacga tttactgcaa aacttcccga accgcgtggt tgccgggctg ctgaatgtgg
    11881 tgatcttccc gaccggacgt cattatctgg caccgtctga caagctggat cataaagtgg
    11941 cgaagatttt acaagtgccg aacgccactc gttcacgtat tggtcgcggt cagtacctga
    12001 cgccaagcga gcataacccg gttggtttac tggaagaggc gttggtggat gtgattgccg
    12061 ccgacccgat tcatcagcgg atctgtaaag agctgggtaa aaatctgcca tttacccgtc
    12121 tggatgaact ggcgcacaac gcgctgacga aggggctgat tgataaagat gaagccgcta
    12181 ttctggtgaa agcggaagaa agccgtctgc gcagtattaa cgttgatgac tttgatccgg
    12241 aagagctggc gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt
    12301 aattgaataa cggagccgaa aggctccgtt tctttatccg ctaattattt aaaattaaag
    12361 ccatccggat ggttttccag gctgccggtc aacgccgcga acagcaccgt tttaccatca
    12421 atcgaaagcg catcgttcac attcagccag gtgagtttct cttgcgacgt tttctcatca
    12481 atagccgaga acagccccgt catctgatta gatttctcgg accacatcac agcgatacgt
    12541 tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct gatacgtctc atctcccaac
    12601 gttacggttt gtgcgggagt ataagtaccg cctttcatca cccatgcagg tagcttatgc
    12661 ccttgcacca tctgattaaa cgcagctttg gtggtttcgc cctttgcaag gctgctaatg
    12721 gttaaatcat cctgcgccat tgcactggtg gcgatgacca gtgcggcgac tgtcgttatt
    12781 gccttaaaca tcattcctcc cgagcttatc ctgcccatgt tattaacctc ctaaagcctg
    12841 gcgataacac gataatttgc aaatcagata tttccaatat cagcatttgt tatcaattta
    12901 ttctgcgcta aggcaactgc ctgataaggt gttaaagtta taaaaccctt cgcataaagg
    12961 agttgtacac cgtgcctggt ttgaagatta cgcttttgca gcaaccgttg gtgtgggtgg
    13021 atggtcctgc caacctgcgc cattttgatc gtcaactgga aggtattacc gggcgcgatg
    13081 tgatcgttct accggagatg tttaccagcg gctttgccat ggaagcggca gcttcgtcgc
    13141 ttgcacaaga tgacgtagtg aactggatga cagctaaggc gcagcagtgc aatgcgctga
    13201 ttgcaggcag tgttgcatta caaacggagt ctggttcggt taaccgcttt ttgctggttg
    13261 agccgggcgg cacggtacat ttttatgata agcgtcatct gttccgcatg gcagatgagc
    13321 atctgcatta taaagcgggc aatgcgcgag tgattgtgga atggcgcggc tggcgtattt
    13381 tgccgctggt gtgctacgac ttacgttttc cagtgtggtc gcgcaatctc aacgattatg
    13441 acctcgccct gtacgtcgcc aactggccag ctccgcgctc tctgcactgg caggcattgc
    13501 tgacggcccg cgcgattgag aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg
    13561 atggcaacgg ctgccattat cgcggtgaca gccgggtgat taatccgcaa ggcgagatta
    13621 tcgctaccgc cgacgcgcat aaggcaacgc gtattgatgc ggagctgtcg atggtggcat
    13681 tgcgggaata tagagaaaag tttccggcat ggcgggatgc ggatgagttt aggttgtggt
    13741 gagtgggggt tgtgtttagg gccgggggct gggctagatt acgaaagccc ctaacttaaa
    13801 cacaatatta cgccagaagt caccatttcc aaaacacagc gtcgctgtac cgtgggttat
    13861 ctgcccaacc gcagcgacac gccggtgatc aaaatcagcg ccaaatggct gagaaaagcg
    13921 ggatttgaga tcggcacggg cgttaccgtg aagatctcgg agggatgttt gatcctgctt
    13981 gggataacaa cgaagtacag gaactgcgca gggaacttta tcaggttaaa cagtcagtta
    14041 aggggatgcg agacgggatg tttagtgtgc tgaacgaaag ttaaataata aaaagccgga
    14101 aagatctgga agaaatttcc ggttattatc gttgctatat c
//
LOCUS       DADKXM010000102        13977 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.103, whole genome shotgun sequence.
ACCESSION   DADKXM010000102 DADKXM010000000
VERSION     DADKXM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.103"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(245..2542)
                     /gene="bglX"
                     /locus_tag="JQR05_002914"
     CDS             complement(245..2542)
                     /gene="bglX"
                     /locus_tag="JQR05_002914"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY5325027.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            2738..4453
                     /gene="dld"
                     /locus_tag="JQR05_002915"
     CDS             2738..4453
                     /gene="dld"
                     /locus_tag="JQR05_002915"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY5325028.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(4491..5423)
                     /gene="pbpG"
                     /locus_tag="JQR05_002916"
     CDS             complement(4491..5423)
                     /gene="pbpG"
                     /locus_tag="JQR05_002916"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY5325029.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5597..6184)
                     /locus_tag="JQR05_002917"
     CDS             complement(5597..6184)
                     /locus_tag="JQR05_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY5325030.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            6354..6932
                     /locus_tag="JQR05_002918"
     CDS             6354..6932
                     /locus_tag="JQR05_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5325031.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(7049..7810)
                     /locus_tag="JQR05_002919"
                     /pseudo
     CDS             complement(7049..7810)
                     /locus_tag="JQR05_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(7863..9299)
                     /gene="mdtQ"
                     /locus_tag="JQR05_002920"
     CDS             complement(7863..9299)
                     /gene="mdtQ"
                     /locus_tag="JQR05_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY5325032.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            9522..9605
                     /gene="yohP"
                     /locus_tag="JQR05_002921"
     CDS             9522..9605
                     /gene="yohP"
                     /locus_tag="JQR05_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY5325033.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(9976..10923)
                     /gene="dusC"
                     /locus_tag="JQR05_002922"
                     /pseudo
     CDS             complement(9976..10923)
                     /gene="dusC"
                     /locus_tag="JQR05_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            11162..11560
                     /locus_tag="JQR05_002923"
     CDS             11162..11560
                     /locus_tag="JQR05_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY5325034.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYILIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            11557..12252
                     /locus_tag="JQR05_002924"
     CDS             11557..12252
                     /locus_tag="JQR05_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY5325035.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            12382..13266
                     /gene="cdd"
                     /locus_tag="JQR05_002925"
     CDS             12382..13266
                     /gene="cdd"
                     /locus_tag="JQR05_002925"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY5325036.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            13416..>13977
                     /gene="sanA"
                     /locus_tag="JQR05_002926"
     CDS             13416..>13977
                     /gene="sanA"
                     /locus_tag="JQR05_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY5325037.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARF"
BASE COUNT         3226 a         3757 c         3530 g         3464 t
ORIGIN      
        1 tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt gataatgaca
       61 gagttagcct gagcggaagg attcagttgc agaatcagat aaatacttaa gaggcatatt
      121 cggtccggcg tttcctgccg gatgcggcgc gagcgcctta tccggcctac aaagggcgaa
      181 aattcagcaa gttaagatat gaacatcagg caaagggcgc aaacgtcgcg cccttactaa
      241 agcattacag caactcaaac tcgcctttct taacgcgtgc ggaatcagtg ccgataaata
      301 cattgaactt gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct
      361 caatatcgat cgggaagctg acagtctgag tttcgcccgg tttcagggtg attttctcaa
      421 agcctttcag ctgtttcaca gggcgactca tggaagccgt cacatcctgc aagtacatct
      481 gcactaccgt ggccccctcg cgcttaccgg tgttcgtcac ctgcacgctg gccgtcactt
      541 tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtat
      601 agctcagacc atagccgaac ggataaagcg ccccgttagc ttcatcaaaa taacgcgaag
      661 tgtatttgtt cggcttgtcg gcattatacg gacgaccggt gttcagatgg ctgtagtaca
      721 ccgggatctg cccgacagaa cgcgggaagg acattggcag cttgccggac gggttgtaat
      781 cgccaaacaa tacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca
      841 gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgcccg ttcatcagca
      901 ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg
      961 gaatagtgat atcggtccgg ctggaggctt cgtgcgccat cccctgtgct tcaccgacta
     1021 cagccaccac cacatcagat tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg
     1081 gcgaacgcgg atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt
     1141 tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggaat
     1201 ttttaatccc ggtcagtacg gtcacggatt gatcggcaac accggctgcg gaccagctgc
     1261 ccatcacgtc acgtttactg tccgccagtg gcccaaccac cgcaatggtg gccgattttt
     1321 tcagcggtaa cgtttcgaga cggtttttca gcaacaccag gctttcgcgc gccacttcac
     1381 gcgcttcttt acggtgcagg cggctttcgg cattggtatc caccgggtca gactctttcg
     1441 gccccaaatg gctgtatggg tcgttaaaca accccatatc atatttaacg ttcagtacat
     1501 ggcgggcagc atcgtccagc tctgccatgg tcactttgcc ggatttaatc aacccaggca
     1561 gatacttcga gtagtactcg tcgctcatac tcatgttgat tccggatttc agcgccacgc
     1621 gcaccgcatc ttccgggtct gccgccgtgc catgtttaat cagctctttg attgcaccgt
     1681 gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc
     1741 aggaatcgga ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcgccgc
     1801 tgcctgcgtc cagccccgct ttgtacggcg gcatataatc attaaacagg cgctgcggac
     1861 tcatatcgac ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt
     1921 tgacgctggt catcaccgag taacgatctg ccgggctttt accctgcatc gcctccacca
     1981 tggttttacc cattattgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc
     2041 cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat
     2101 ctgccgcttc ataagcagag acacgcccga ccgttttcac cgcatcgagg ttaaaagacg
     2161 aggccagacc gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa
     2221 agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat
     2281 cctgacgggt tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga
     2341 tcgcctcttt cggattatcc gggcctacgc tgattaagcg cagctgacca attttctcat
     2401 caaccgtcat tttcttgagc agatcggtga cgaacgcatc ccgcgcttca ggcgttaatg
     2461 gatggttacc gaataaatca tccgccagtg ctggctgcag gaccagactc accgcgattc
     2521 ctactgaaca tagccatttc atatggattt ttcttccttt gttgcccgac gtggcagcga
     2581 aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag
     2641 tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta
     2701 gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga caacaactga
     2761 taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc tgctcaccga
     2821 tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc
     2881 tgtcgttttc cctggctcac tactggaatt gtggcgggtg ctgaaagcct gcgtcaccgc
     2941 cgacaaaatt attctgatgc aggctgccaa tacaggcctg accgaaggat cgacgccaaa
     3001 cggtaacgac tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca
     3061 cgttctcggc aagggcgaac aggtgctggc ctatccgggc accacgctct attcgctgga
     3121 aaaagccctc aaaccgctgg gacgcgaacc gcattcggtg attggatcat cgtgtatagg
     3181 cgcatcggtc atcggcggta tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc
     3241 ggcgtatacc gaaatgtcgt tattcgcgcg tatagatgaa gatggcaaac tgacgctggt
     3301 gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga
     3361 cgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg attatgacta
     3421 tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg ccgatccgga
     3481 tcggttattt gaatcttctg gttgcgccgg gaagctggcc gtctttgcgg tacgtcttga
     3541 taccttcgaa gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc agccggaagt
     3601 gctgaccgaa atccgccgtc atattctggc taacttcgaa aatctgccgg ttgccgggga
     3661 atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat
     3721 gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga
     3781 tgcgatgctg gagaaagtga aattcttccg tccgcatttt actgaccgtg caatgcaaaa
     3841 attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata
     3901 cgagcatcat ctgctgttaa aaatggcagg cgatggcgtc ggtgaagcca aatcgtggct
     3961 ggtggattat ttcaaacagg ccgagggcga tttctttgtc tgtacgccgg aggaaggtag
     4021 caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggcggtgca
     4081 ttccgatgaa gtcgaagaca ttctggcact ggatatcgct ctgcggcgta acgacaccga
     4141 gtggtatgag catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg
     4201 ccattttatg tgctatgtct tccatcagga ttacatagtg aaaaaaggcg tggatgtgca
     4261 tgcgttaaaa gaacagatgc tggaactgct acagcagcgc ggcgcgcagt accctgccga
     4321 gcataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagttct atcgcgagaa
     4381 cgatccgacc aatagcatga atccggggat cggtaaaacc agtaaacgga aaaactggca
     4441 ggaagtggag taaaaattac ggatggcaga gtatcgccat ccgaattcac ttaatcgttc
     4501 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     4561 gctgccggca caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     4621 gcaaaatggg tatatttgcc aaacgcgtcc atcactacca gcgccaccgg tttattattg
     4681 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaagccggt tttggttaac
     4741 tgaatattcc agttatcgcg atacaccaga tgattagtat tgcggaacgg cagcgtatac
     4801 gtcgggttag agaaggttgc catatcttcc cgggtagtac ttaactgccc gatcaacgga
     4861 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     4921 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     4981 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc
     5041 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     5101 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     5161 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
     5221 atagacgcaa tcggacgcac cagatccggg tggttcgaat agatcacttt gttggtattc
     5281 agatcaacaa tcatcgcgct accggaggca atttccggtt gtgaagcggt ggtagcggct
     5341 gccgttttcg caacggcctg cggtgcaaaa ggcacagcca gcatcagggc caggctaaat
     5401 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacgggttg
     5461 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
     5521 cgatcgtcaa aggagaatcg tgagtaaatg ctgcattgct gacatttacg ccagcaatgc
     5581 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
     5641 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactggag aagctaaggc
     5701 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
     5761 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
     5821 gaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagag
     5881 acggacgctg cgggtaattg cgcgccatcc accagatgac ccgccccatc accgcaaccc
     5941 cagccagcat aacgccgtaa aacaagacag ccagcgccag tccggtaaac caggataact
     6001 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
     6061 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
     6121 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
     6181 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gccagcttgt
     6241 gattattttt tgttcatcca tgtaatttgc accgtctgat gtatgtcctt tttcagcgtg
     6301 aatgattcac agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
     6361 tcaatacact catctcacaa tatggttatg ccgcgctggt tatcggtagc ctggcggaag
     6421 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccac
     6481 tggtggtact ttctgtggcg cttggtggca tgattggcga ccaggtgctc tatctgtgcg
     6541 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
     6601 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
     6661 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
     6721 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
     6781 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
     6841 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
     6901 gcgggaagaa aaagccggat aatcaggcgt aaagcagttg ccacagataa gcattcactc
     6961 cacatccggc accgggtgtt ccatgcctga tgcgacgctg atgtcttatc tggcctaacc
     7021 cattgaaata tccacgcacc tcactgcgct attctgggtt gaactgcgga ttcgccaaca
     7081 taaagccgcc atccactatc aacgactgcc ctgtcgtgta atttgcgccc tccgaacaaa
     7141 gccacaccac caagctggca atctcatgcg ttgcgccaaa acgccgtaga ggaatcgaag
     7201 gctccgcgtc gggcttcacg tcgctatcat ccatgccgtt cattggcgtg gctatcgccc
     7261 cagacgcgac tgcgttcacc aaaatcttat gcctgaccaa ctccagcgcc atcgttttgg
     7321 ttaacccgcc gagcgcatgt ttagcggctg tgtaggcgct ggcatccggc agcggcgtat
     7381 gttcatgtac cgacgtaatg ttgatgatgc gaccgccctg cccttatttc accatctgac
     7441 gagccgcaat ttgcgagcat aaaaatgcac catcaacatc aacggtaaaa atcttgcgcc
     7501 actcatcaaa agccatatca agaaacggcg ctttggtcat tgcacccgca ttattcacca
     7561 gcacatcaat acgcccaaat cgctggatga gtgtctccag cgcctgcgca ccttctggca
     7621 gattgccgag atccagttgc acgatctccg cacgtacgcc gtgggtaacc acctcacgcg
     7681 cggtatcttt tgccccttct tcatctgagt gccaggtaat gccaatatca aacccctgct
     7741 gcgccagtaa taacgcgcac tctttgccga tccccgaatc ggaagcggta attatcgcga
     7801 cctatgccat cgagttctcc atttaacgct gaataaacgt taagtataga aggcgcatat
     7861 catcagcgtt tgtaaccccc gcccagcgca ccggtgagtt gaatggaggc atccagccac
     7921 tgcccttgca ataacaggcc attggcccgc tcacgcagcg ccgggattct ggcttcgctg
     7981 acgcgggaac cagcaatgat gcccgcgtta aagcgggcct gcgcaagacc taccacacgc
     8041 aaggcatcgc gctcaatttg cgcctgatgc tggtttttct ccgccagtgt ctgaacctga
     8101 ctggctgccc gcgccacgtc attcactgct tcaaccaccg ctttgttgta gctggcgata
     8161 gacaagttgc tttcggcttt tgcgatatcg agattggcgt taagacgacc actatcgaaa
     8221 ataggtagcg tcaggcctgc cgtaacgccc atttgctgcg cggaatgacg gaacagatcg
     8281 cttaagtgca acgcatcctg ttgcaggaag gccatcaggt tgatgtcagg ataaaatgcc
     8341 gcttttgccg catcaatagt gcttaacgat gactcaacgt accagtgcgc cgcctgcaaa
     8401 tctgcccgcc gggccagtaa ggagtacccc agttcatcag gaagctggct tgccactttc
     8461 ggcaacgcga ccggtttaag cttcaatgac ttagtctggt gatttgtgag tgcatttaac
     8521 cgtgcctcaa taattttcat tttccccgcg acatcgttga gctgctgccg ggttttgctg
     8581 gcattaatat cggtttccac accgtcaact gaagaagtaa tcccgttctg atatagctgg
     8641 cgatcggtcg cgataatggt gttctgctct ttttctattt gctgcaagac cgtgtttaac
     8701 gccgcctggg tttgccactc ccagtacagg cgggctacgc tgccagccag caattggcgg
     8761 gtttgctcgc gttccgccgc ccgtgcttta accgtaccca ggcgggcagt aacctccgcc
     8821 cgattctttc cccagatatc gagatgccag cccgccgtta agccaaaagt tccgttggtg
     8881 taccacggac cggtcgtacc tgcggccgga tcgttcagag caaacggccc cattagccct
     8941 tctgccgaca ttttttgccg ctccatatcc gccgaaaagt cgatctgcgg accatcctga
     9001 gtggcaactg ccttcgcctg ggcttcagct agctgaatgc gctgttcagc cacctgcata
     9061 tccggtgcgt tctgtagtgc attgttaatt aatgaagtga gctgattatc gtgatactcc
     9121 agccaccatt ggctgtctgg ccaaccattt ttcagcgccg tgggtaatgc ggtgtcaact
     9181 tgtgcagcgg gcgtttgctg gcttaacgcc tggcgggttt catgcatagg cgcacacccg
     9241 gccagcatca gtaacagcgg aaaacaggcg atggctggat aaaaggaatc acgattcatg
     9301 gggaataatc aggtaagaaa cggtgcgcgg agattaccgt gtgttgcgat atatttttta
     9361 gtttcgcgtg gcaatacatc agtagcaata aaacgacata cccagaaaaa tatagactaa
     9421 gtaaatgata tcttccgatt tatcttaatc gtttatggat aacggcaaag ggcttcgttt
     9481 tttcctatac ttattcagca ctcacaaata aaggaacgcc aatgaaaatt atactctggg
     9541 ctgtattgat tattttcctg attgggctac tggtggtgac tggcgtattt aagatgatat
     9601 tttaaaatta attaatgtca tcagatccga aaataacgag aatatttcag tctctcatcc
     9661 tgttgcgctc ctgtcatgtg cattgcttca tataatcact ggcgcaagga gcgcgcaggg
     9721 ggcgaccaat cgccgccgcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc
     9781 gcggtgccct cggcttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta
     9841 aaacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggcg
     9901 attttgacgc caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa
     9961 acatcactat ggtttttaga gtttctcgat atcaatttcc tgaatagccc ttgcaatatc
    10021 aggggagtta ttcaacaccc gaacatgctg aaataattcc gttgcttcat cgtattcttt
    10081 acgcaaataa ctcaaccact gtttaatccg cgcaacgtga tataacccgg tatcgccctg
    10141 cttttccaga cgggtatatt tttgcagcaa agcaaccacc tccggccacg gcattcgcgg
    10201 ttcgttatat tttaccaccc ggctcaggtt gggaatattg agcgccccgc gaccaatcat
    10261 cactgcgtcg cagccgctga tcgccatgca ttgttgcgcg ctctgccagt cccagatttc
    10321 accgttggca atcaccggaa tattcagccg ctggcgaatc tcgccaatcg cctgccagtc
    10381 aatatgctcc gcgcggtaac cctgctcttt cgtccgccca tgcaccacca gctccgtagc
    10441 gccagcctgt tgaaccgcat cggcgatttc aaatttcttc tcaccgctgt cccagcccag
    10501 acgcactttc acgctgacag gcaaatgcgc cggtacagct tcacgcatcg cttttgcccc
    10561 ctagtagatg agttcaggat ctttgagtaa cgtcgccccg ccgccgctac cgttaaccgt
    10621 tttcgacggg cagccgcaat tgagatccac gccccaggaa cctaactcca ccgcacgggc
    10681 ggcgttctct gccagccatt gtaggaactg acctaacaac tgcacgcgca ccagcgtacc
    10741 agatggtgtc cggctggcgt tttgtagctc agggcaaatg cgatgaaaga cttttaccgg
    10801 cagcagttga tccaccacgc ggacaaactc ggtgatgcac agatcgtagt cgttaacttc
    10861 ggtcagcaat tcacgcacca gagagtcaag cactccctcc atcggtgcca gtaacacacg
    10921 catatcatca cccgcaaaaa aatgaggcgc tatgttagcg cctatggtca gcggattaaa
    10981 ggtctgcaaa gagggattgc tgcaaactgc aatccctctc tatgcttgat gtatgcctga
    11041 gttcgctgct cattgcgtaa taccgggtag cgaaaaaata agcgctgatt tcttaatgtg
    11101 atcggtagca cgttttacga attaattgta tgatgaatcc atctcatctg gggtgttgat
    11161 tatgagtaag acactgaaca ttatctggca atatttacgc gctttcgtcc tgatttatgc
    11221 ctgcctgtat gcaggcattt tcattgcttc cctgctaccg gtaaccattc cgggcagcat
    11281 catcgggatg ctgatcctgt ttgtcctgct ggccttgcaa attcttccgg cgaaatgggt
    11341 caatccgggg tgctacatac tgattcgcta tatggcgcta ttgtttgtgc cgattggcgt
    11401 aggcgtcatg caatattttg atttgctccg cgcgcagttt ggcccggtag tggtttcctg
    11461 tgctgtcagt acgctggtgg tttttctggt ggtgagctgg agttcacaac tggtacacgg
    11521 tgaacgtaaa gtcgtaggtc agaaaggatc agaagaatga tggcgaatat ctggtggtca
    11581 ttaccgctga cgttgattgt cttttttgcc gcccgcaaac tggcggcacg gtataaattt
    11641 cccctgctta acccgttgct ggtagcaatg gtggtgatca ttcctttttt aatgctgacc
    11701 ggcatctctt acgacagtta ctttaaaggt agcgaagtgc taaacgatct gctgcaaccg
    11761 gcggtcgtcg cgctggccta tcctttatat gagcagctac accagatccg cgcgcgctgg
    11821 aaatcgatca tcaccatctg ttttatcggc agcgtggttg cgatggtgac ggggacttcc
    11881 gtggcattgt tgatgggcgc ttcaccggaa atcgccgcgt caatcctgcc gaaatcagtc
    11941 accacgccta ttgcaatggc ggttggcggc agtattggcg gtattccggc aatcagcgca
    12001 gtttgcgtga ttttcgtcgg catcctcggc gcggtatttg gccatacatt gcttaatgcg
    12061 atgcgtattc gtaccaaagc tgcgcgcggt ctggcaatgg ggactgcctc gcacgccctc
    12121 ggtacggcgc gctgcgccga gctggattat caggaaggtg catttagttc gctagcgctg
    12181 gtgttatgcg ggataattac ttcgctgatc gcaccgttcc ttttcccgat tattctggca
    12241 gtaatgggct aaaatttgcg atacgtcgcg catttttgat gtaagtttca cgtgttgcat
    12301 aattaatgag attcagatca catataaagc cataacgggt tcgtaaactg ttatcccatt
    12361 acatgattat gaggcaacgc catgcatcca cgttttcaaa ccgcttttgc ccaacttgcg
    12421 gataacttgc aatctgcact ggaacctatt ctggcagaca agtacttccc cgctttgttg
    12481 accggggagc aagtctcatc gctgaagagc gcaacggggc tggacgaaga cgcgctggca
    12541 ttcgcactac ttccgctggc ggcggcctgt gcgcgtacgc cattgtcgaa ttttaatgtt
    12601 ggcgcaattg cgcgcggtgt gagcggaacc tggtattttg gtgccaatat ggaatttatt
    12661 ggtgcaacaa tgcagcaaac cgttcatgcc gaacaaagcg cgatcagcca cgcctggttg
    12721 agtggtgaaa aagcgcttgc agccatcacc gttaactata cgccttgtgg tcactgccgt
    12781 cagtttatga atgaactgaa cagcggtctg gatctgcgta ttcatttgcc gggccgcgag
    12841 gcacacgcgc tgcgtgacta tctgccggat gcctttgggc cgaaagatct ggagattaaa
    12901 acgctgctga tggacgagca ggatcacggc tatgcgctga ctggtgatgc gctttctcag
    12961 gcggcgattg cagcggcaaa ccgttcgcac atgccttaca gtaagtcgcc aagcggtgtc
    13021 gcgctggaat gtaaagacgg tcgtattttc agcggcagct acgctgaaaa cgccgcattc
    13081 aacccgactc tgccgccgtt gcagggggcg ttaattctgt tgaatctcaa gggttatgat
    13141 tacccggata tccagcgcgc ggttctggca gaaaaagccg atgcgccgtt gattcagtgg
    13201 gatgccacct ccgcaacgct gaaagctctc ggctgtcaca gtatcgaccg cgtgcttctc
    13261 gcttaagcct ggtgccggat gcggcgtgaa cgccttgtcc ggcttgccag ccctctcctg
    13321 gtgtcgaaat tcccggcaaa cagtttgccg tttcttgcgc aaaaccagcg ggtaaagtag
    13381 cctgatggaa attttcctta gctcgagtct cctgcatgtt aaagcgcgtg ttcctcagcc
    13441 tgttagtcct gatcggcttg ctgctgttga ctgtgctcgg cctcgatcgc tggatgagct
    13501 ggaaaaccgc gccttatatc tacgacgaat tgcaggatct cccctaccgc caggtcggcg
    13561 tggtgctcgg cacagcaaaa tattatcgta ccggcgttat taatcagtat tatcgctacc
    13621 gcattcaagg agcgattaat gcctataaca gcggtaaggt aaattatcta ttactgagcg
    13681 gcgataacgc attgcaaagt tataacgagc cgatgaccat gcgcaaagat ttaatcgctg
    13741 ctggtgtcga cccatcagat attgttctcg attacgcagg ctttcgtacg ctggattcca
    13801 tcgtgcgtac acgcaaagtt ttcgatacta atgatttcat tattatcacc caacgtttcc
    13861 actgtgagcg agcattattt attgcgctgc atatggggat tcaggctcag tgttatgccg
    13921 taccgtcacc gaaagatatg ctgtcagtac gtattcgcga atttgccgcc cgtttcg
//
LOCUS       DADKXM010000103        13893 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.104, whole genome shotgun sequence.
ACCESSION   DADKXM010000103 DADKXM010000000
VERSION     DADKXM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.104"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            80..1432
                     /locus_tag="JQR05_002927"
     CDS             80..1432
                     /locus_tag="JQR05_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY5325038.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNSAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLICSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1445..2203
                     /gene="chbG"
                     /locus_tag="JQR05_002928"
     CDS             1445..2203
                     /gene="chbG"
                     /locus_tag="JQR05_002928"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY5325039.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2250..4501)
                     /gene="katE"
                     /locus_tag="JQR05_002929"
                     /pseudo
     CDS             complement(2250..4501)
                     /gene="katE"
                     /locus_tag="JQR05_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            4684..4947
                     /gene="cedA"
                     /locus_tag="JQR05_002930"
     CDS             4684..4947
                     /gene="cedA"
                     /locus_tag="JQR05_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY5325040.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5224..6040
                     /locus_tag="JQR05_002931"
                     /pseudo
     CDS             5224..6040
                     /locus_tag="JQR05_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6044..7435)
                     /locus_tag="JQR05_002932"
     CDS             complement(6044..7435)
                     /locus_tag="JQR05_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY5325041.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     DSFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSSSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(7568..8158)
                     /locus_tag="JQR05_002933"
     CDS             complement(7568..8158)
                     /locus_tag="JQR05_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5325042.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8321..8989)
                     /gene="hxpB"
                     /locus_tag="JQR05_002934"
     CDS             complement(8321..8989)
                     /gene="hxpB"
                     /locus_tag="JQR05_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY5325043.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     EQPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            9136..9672
                     /locus_tag="JQR05_002935"
     CDS             9136..9672
                     /locus_tag="JQR05_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325044.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLRESVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9713..10573)
                     /locus_tag="JQR05_002936"
     CDS             complement(9713..10573)
                     /locus_tag="JQR05_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY5325045.1"
                     /translation="MWQAIIRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPHRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10679..10969)
                     /gene="ghoS"
                     /locus_tag="JQR05_002937"
     CDS             complement(10679..10969)
                     /gene="ghoS"
                     /locus_tag="JQR05_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY5325046.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11070..11999)
                     /gene="pfkB"
                     /locus_tag="JQR05_002938"
     CDS             complement(11070..11999)
                     /gene="pfkB"
                     /locus_tag="JQR05_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY5325047.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12286..13044
                     /locus_tag="JQR05_002939"
     CDS             12286..13044
                     /locus_tag="JQR05_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY5325048.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13097..13204)
                     /locus_tag="JQR05_002940"
     CDS             complement(13097..13204)
                     /locus_tag="JQR05_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325049.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13376..>13893)
                     /locus_tag="JQR05_002941"
     CDS             complement(13376..>13893)
                     /locus_tag="JQR05_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325050.1"
                     /translation="IIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMEN
                     NHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYI
                     STLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYAAINVISQSL
                     SFSADEIKTYL"
BASE COUNT         3574 a         3203 c         3430 g         3686 t
ORIGIN      
        1 tctgtcctgt tgctggatta tttatgtccg ggggggcaga tatgccagat atcagtattc
       61 tgtactgaag ggagaaatta tgagccagaa attaaaagtc gtcactattg gtggcgggag
      121 cagttatacc ccggagttac tggaaggatt tattaagcgt tatcacgaat tgccggtcag
      181 cgaattatgg ctggtggatg tcgagggtgg caaagctaaa ctggatatta ttttcgatct
      241 ctgccaacgg atgattgata acgctggcgt cccgatgaag ctttataaaa cgctggatcg
      301 ccgcgaagca ttgaaagatg ctgatttcgt tactacccaa ctgcgcgttg gccaattacc
      361 ggcgcgtgaa ctggatgaac gtattccatt aagtcatggt tatcttggtc aggaaaccaa
      421 cagcgcgggc ggtttgttta aaggtctgcg taccattccg gtgatttttg acatcgtaaa
      481 agatgtcgaa gaactttgtc cgaatgcatg ggtgattaac ttcactaacc cggcgggaat
      541 ggttactgaa gccgtttatc gtcataccgg atttaaacgc tttatcggcg tgtgtaatat
      601 tccgatcggc atgaagatgt ttattcacga tgttctgatg ctgaaagaca gcgatgattt
      661 atctatcgat ttgtttggcc tcaaccatat ggtgttcatt aaggatgtgc tggtaaatgg
      721 caagtcgcgc tttgccgaat tgcttgatgg tgtggcatcc gggcagttaa aagcatctgg
      781 cgttaaaaat attttcgatc tgccatttag cgaaggctta atttgttctc tgaatctgtt
      841 gccgtgttct tatttgcttt attacttcaa gcaaaaagag atgctggcta ttgaaatggg
      901 cgaatactac aaaggcggcg cacgagcgca ggtagtacag aaagtcgaga aacaactttt
      961 tgagctgtat aaaaacccgg agttgaaagt taagccgaaa gaactggaac agcgcggtgg
     1021 ggcttattac tctgatgcag cgtgcgaagt gatcaacgct atctacaacg acaagcaagc
     1081 tgaacattac gttaatatcc cgcatcatgg gcatattgat aatattccgg cagactgggc
     1141 ggtagaaatg acctgtaagc tggggcgcga tggcgcgacg ccacatccgc gcattacgca
     1201 tttcgatgat aaagtgatgg ggctgattca caccattaaa ggcttcgaga ttgctgccag
     1261 caacgccgca cttagcggag aatttaacga tgtgttactg gcgctaaacc ttagtccgtt
     1321 ggtgcattcc gatcgcgatg ctgagctgct ggcacgcgag atgattctgg cgcacgagaa
     1381 atggctgcca aattttgccg actgcatcgc agagcttaaa aaagcacatt aaccgaggct
     1441 gattatggaa cgcttactga ttgttaatgc cgatgatttt ggcttaagca aaggtcagaa
     1501 ctacggcatt atcgaggcct gtcgcaatgg gattgtcaca tcgacgacgg cgctggtgaa
     1561 tgggcaggct attgaccatg cggtgcagtt gagtcgtgat gaaccgagtc tggccatagg
     1621 gatgcacttt gtccttacta tgggcaagcc actgacagct atgccggggt taacccgcga
     1681 tggtgtgctg ggaaaatgga tctggcagtt ggcagaagaa gatgctttac cgctggaaga
     1741 aattactcag gagcttgcca gtcagtattt gcgtttcatt gagctatttg gacgcaaacc
     1801 tacgcatctt gatagccatc atcatgtgca tatgttcccg cagattttcc cgattgttgc
     1861 caggtttgcg gctgaagagg ggattgcgtt gcgcatcgac cgtcagccgc tatctaacgc
     1921 tggtgattta ccggctaatc tgcgcagttc gcagggattc agtagcgcgt tttatggtga
     1981 agagattagc gaggcactgt tcctgcaagt gctggatgac gcaagccatc ggggtgatcc
     2041 ttcgctggag gtgatgtgtc atccggcgtt tatcgataat actatccgtc agagtgctta
     2101 ctgtttccca cggttaacgg agctggaagt gctgacatca gcgtcgttga aatatgcaat
     2161 tgctgagcgt ggttatcggt tgggtagtta tcttgatgtg taagtaaggt ggtaaggtca
     2221 ctccccaggg gacgtgacct tatccatatt tacgccggaa ttttgtcaat cttaggaatg
     2281 cgtgaccaca cgcggtgttc tgccatcagc gttaacagtt catccataaa actaccgtcg
     2341 gcgctgtcag cttccacaat cccttcttca ccctggtcag ctaccttgat tgttgcttta
     2401 aacttgcgcg cgtctcccgc cagcgcaatc ggtttaaggt gtttgtaggc ttccatcaga
     2461 tagtagttgg catcgccgtt gtcagcgata tccgcgatat tgccgcaagg gacaatgacc
     2521 gcatcgaccg tcagcgaagg tgcaccggca aaggtagcgg ctataggcaa caccgtaccg
     2581 tcatccgcag tcacttcacc cactcgggag tagagcagtt tggcatgaac gcctttggcc
     2641 ttcagggcct tgagaatggc cagaaggtct gccgatctca cttcatcatt aagtaaaatc
     2701 gctaccacgc gacctttcac atcaccgtca ggaatggcgt acaaacttaa ggatggatcc
     2761 tttttcagac cgttgacgtc cggaggtggg gtgatattca gctggtcgtc agtcagttcg
     2821 ataccgagat ttttcgccac cgcctgggcc agagtgagat caatatgcgc cagctggtca
     2881 acaacgcgct cacgaatata cggacgaacg actttgctta actcaaaact gaaaccatcg
     2941 acaatatggc gctgctcgaa tggcgtctga cttagccaga acagacgcgg atgggaataa
     3001 tattcgccaa acgatgggct gcgctcgcga actttattgc cttccacgcg ctcctggtat
     3061 gattcaaaac cgccgcgttt cggccccggt ggtgtttcgc gcggccagtt atcgttgatc
     3121 gagttcggtt cataattcgc cgggttagtg tcgatcccca tacgatgcat gccgtcacgc
     3181 tggaaattat ggtaagggca ggtcgggcgg ttaatcggaa tctcatggaa attcggccca
     3241 ccaagacgac tgatttgtgt atcggtatag gagaacaaac gtccctgcaa caggggatcg
     3301 ttggtgaagt ccagaccagg aacaatatga cctggatgga atgctgcttg ttcgttttcg
     3361 gcaaagaagt tatccggatt gcgattgagc accattttgc cgacacgctg aacgggcacc
     3421 agttcttccg ggatcagttt ggttggatcg agaagatcga agtcgaactt gaattcatct
     3481 tcttcaggaa tcaactggaa gcccagttcg tattccggaa aatcgcctgc ttcaatggct
     3541 tcccacaact cgcggcggtg gaagtccggg tcacgtccgg tgagtcatcc caaacgagtg
     3601 aggctttacc tgccagtggt ttccagtgga aacgtacaaa cgttgccttc ccttcggcat
     3661 taatcaggcg gaaggtgtga ataccgaagc cttccatggt gcggtaactg cgggggatgc
     3721 cgcgatccga catcgcccac atcacgttgt gcagagtttc aggttgcaga gaaacataat
     3781 cccagaaagt atcgtgggca ctttgccctt gtggaattgc ccagtgcggt tctggtttta
     3841 ccgcatgaac aaaatcgggg aatttatgcg catcctggat aaagaagatt ggcgtgttat
     3901 tgccaacgag gtcaaaaata ccctcttcag tatagaactt ggtggcaaaa ccacggatat
     3961 cacgcacggt atcggcagag ccagcgccac cctgaacggt agagaaccta acaaatactg
     4021 gggtgatttt gttcggatct gagaggaaat ccgctttggt gatatcgctt aagcttttat
     4081 atggctggaa ataaccgtga gctgctgatc cgcgtgcatg aacaatacgt tccgggatgc
     4141 gctcatggtc aaagtgggtg attttctcgc gcagaataaa atcttccagc agcgttggac
     4201 cacggttacc ggcacgcagt gagttttgat catcggcgat gcgcacgccc tgattagtgg
     4261 tcagcgcata attttcactg cctttgcgta cgtcttccag agaattaagt ttttcgttac
     4321 gtgtatcagg ggctttcagg ctcccagggg cggtaggttg tgcacctggc ggtgttggtt
     4381 cagccgctgg acgatgagat ccgtcctcag gtgccagtga gtccatcccc ggtttcgctt
     4441 cgctggaatc gtgtagtggt gactggtgct gatgtgggtt cttttcgtta tgttgcgaca
     4501 ttgaactcgt ctccttaatt tattactgaa agggccgctg ttttggtcaa aaaactctct
     4561 aactatagac caccagccag atcccgcttc ggagactatt ctggaaacgg tgttatgaaa
     4621 tacacgcaaa gcgggacgta caggggctaa atcggctaaa ctacagtttt tctaattttt
     4681 aatatgcgtt tagtgaagcc agtaatgaag aaaccgctcc gccagcaaaa ccgccagatt
     4741 attagctatg tcccacgcac ggaacccgcg ccgccagaac atgcgataaa gatggattcg
     4801 tttcgtgatg tctggatgct gcgtggcaaa tatgttgcgt ttgtactgat gggagagtca
     4861 tttctgcgct caccggcgtt tactgtgcct gaatctgccc aacgttgggc aaatcagatc
     4921 cgccaggaag gggaagtgac tgagtaagga ttacatacct cccggttttg ccgggagtta
     4981 catgacctga aattctgcat ttcgttgcta cattcattca aaccacctgt aacaaaaagc
     5041 tgcctttgac gagctattgc tataaataag tgatcttttt tctttaattg tttatttatt
     5101 ttaaaatggt taagttttgc taatcatgat gagctatttt tgatagttca tcctgtggcg
     5161 tcgctcttaa acttatattg taaatgactt agatttaaat aagataataa ttttgttaac
     5221 aatatgaggc gggagatgtt ttctacaaac ttaatacaga gtaattatgg tgatttaaat
     5281 attaaaagcc tggctttcga ctcttttaaa gaaaggcttg agtctactat ggcggcactc
     5341 actttcttta tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc gaacttcaat
     5401 cgtatgattg cctatatgtc taacattaac tatgatgcct caaagctagg agcgccagca
     5461 cttagttttg atacgtatct gcaggacaat gttaaatata gagtgataat taataatctt
     5521 tatggcagtg agtttcggat aagagatatt aatgagaatt ttgttgggat ggattttacc
     5581 tgtgaattcc gacccgaaaa aatgacgaat ttgatcaata taaaaaatag gttggttatt
     5641 cattatttaa ggactatata ctatgaaaaa aattatatac atcctgccgg gagcattttt
     5701 gctgcgattc aaaaaaaatg agagtctcct aaagtttcct tcgataatat caatgcttaa
     5761 tatcaatctt ctttttaatc cactaaatct tccgggaatg ggaagtggtg tgcttgaaga
     5821 tattatgagt attccagata gttcacttag aaaacgtctc gggtatgagg ttttaagctt
     5881 ctcactacag gcacattcat tgagccagga atgtattgat aagttggaca tttttttcgc
     5941 tgacgatctg tttaaatatg agtcagtatg catcgccgcg atggaacatt taaaaagtaa
     6001 agcaacggcc cctatacaaa acggaccgtt gccagcataa gaattaacgg tgtgccagtt
     6061 cggcgtcgtc ttcgctatcc agaatggctt tatcggtttg cttcagccac tggctggtca
     6121 gcgtgccagc tgtcatcgag ctactaacgt ttagcgccgt acggcccatg tcgataagcg
     6181 gttcaacgga gattaacagc gccaccaggg ttactggcag gcccatcgca ggcagcacaa
     6241 tcagtgcggc gaaagttgca ccaccaccga caccggcaac gcctgcggaa ctaacggtaa
     6301 caataccgac cagcgtcgca atccacatcg ggtccagcgg gttaatgcca accgtaggcg
     6361 caaccatcac cgccagcatt gccggataca aaccggcgca gccgttctga ccaatggttg
     6421 caccgaaaga gtcggcgaaa ctggcgatgg attcaggaac gcccagacga cgcgtttgtg
     6481 cttccacatt cagtgggata gacgcagcac tggaacggct ggtaaaggca aacgtcagca
     6541 caggccatac cttacggaag tacttcagcg gactcacgcc attaatgccc agcagaatgc
     6601 catgcactgc aaacataatc agcagaccga ggtaggacgc gacaacgaaa cttcccagtt
     6661 tgatgatgtc ttgcaagtta gaacctgcaa ccactttggt cattagagcc agaacgccgt
     6721 aaggggtcaa ctgcatgaca aggcgaacca gtttcatcac ccagctttgt agggtatcga
     6781 tagcggttaa gacgcgttca cctttcggcg catcatcctt cagcagtttc aatgcagcta
     6841 cgccgaggaa tgcggcaaaa attaccacgc tgataattga cgtcggattg gctccggtaa
     6901 gatcggcaaa cgggtttttc gggataaagg acaagaccag ctgcggaacg ctcagatcag
     6961 agactttacc aacatagtta ctttcaatgg cgttcagacg tgcagtttct gcaccaccct
     7021 gaaccagacc ttcagccgtc aaaccaaaca ggttggtgac cagcacaccg accagcgccg
     7081 caatcagcgt ggtaaacaaa agcgtaccga tggtcagaaa actgattttg cctaactgag
     7141 atgcgttatg cagacgggca accgcgctca gaatagaggc gaagactaac ggcataacga
     7201 tcatttgcag cagttgaaca tagccgttac caacgatgtt aaaccactgt acagaatctt
     7261 taagtacctg gctgtcagaa ccataaatgg tatgcagggc aaggccaaaa accacaccca
     7321 tcaccagacc caccagcact tttttcgcca gactccactg tttatggcgg gtctgagcca
     7381 gcgcaaacag cagtacaacg aacaccacga tgttcgcaat taatggaaag ttcatccccg
     7441 ttctcctgat cttattattt ctatcactgc gtagcagtca tagtgttggc gcaagattaa
     7501 cagaactgtg agctggctct tatattcaaa tggaattgct tattccagaa aggtgatttt
     7561 tgccttttta gtattcaacc tgatgcttaa taagccgatg aaactggatc tgcaaggtat
     7621 tgatcatttg cgatgaccaa cgaacagcgc tgttctcggg taatgaatgg ggcatccata
     7681 ccgaccagac aaataatgcc atgcagataa aacgttccag ttggttgccc ttttggggaa
     7741 ccaggatagg caagcggaaa cgccagcggc atggccagag caggggaaca ccggcgggtg
     7801 tcagcatatc ggcaagtatg tggctcaaat aacccagcac cattccttgt agcgcatcag
     7861 ccggaatgaa ccagccttcc ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg
     7921 ccagcagact gtgggtaaaa ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc
     7981 attttaatcg ctgcccaaga aacgactttg ggtgatcgat gtccggtaac aaacacgtca
     8041 ggattgcgga agggacaata tgccaccagt caccctgtgc cagcacgggc gtcagctcgg
     8101 catttttggc aaataccgca caagcaatag aaaagagaag gtgaccttcc gccgtcatga
     8161 taaaactcac taaactgtcg atacgtacag tataggattt ttatacagtg cacggaagtg
     8221 gtgacggtgt aactctttct cttaaattga cgattaatcc gtcagttgaa tgcaaattca
     8281 gataaagaac caggggcagc gtgatcactg cccctggtca ttaaccgaga aggtcttttg
     8341 cggtgagttc tgtcagcgat gagagtttga cgtttgctaa tacaaaacgt ggatcatttt
     8401 gcgcttctgg cgcaggaacg acgatggaac gcatgcgggc tgctttagaa gcgatcatgc
     8461 catttaccga gtcttccagc gccacgcagg tcagtgggtc aacgcccagt tttgctgcgc
     8521 agtcgagata tacttgcgga tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg
     8581 catcgaaact gtcgcgtaag tcaaacatgg tcaacacttt ttccagcata tgtagtggtg
     8641 acgcggaggc cagtcccacc aataaacctt gttctttgca taacgcaaca gcttcgcgca
     8701 cgcctggtaa taatggacgt gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt
     8761 ctactacttc ctgacggctt ggcccattcc acggttgccg ggcgtaccaa agatcgacca
     8821 ccatatcgat gcgtaaacct aaggtgtccg gctgctcgtt acgacgggag atatccaccc
     8881 ccaggcttgc catcacatcc agttcggctc gatcccataa aggttctgag tcgataagta
     8941 atccatccat atcaaaaatt gcagcaagaa tctgacgcgg ggttgacatt acaacctctc
     9001 ctttagagca ttatgtgtaa ggtattaagc cgtgcttatg attaagcgcc tatcatatcg
     9061 cctttaaaga atgggcgaaa acagatatct acaggtaaac taagtgaaac gacgcgtctt
     9121 aatcaaggag aactcatgac gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt
     9181 ttgggttggg tgatttttat acctgcattg atctctacat tgatttcttt gctgaaattt
     9241 atgaataccc ggcaggaaaa ccaggaaggc attaatgcgg tcatgctcga ctttgctcat
     9301 gtcatgatcg atatgatgca ggcgaatacg ccttttttga atctgttctg gtataactcc
     9361 ccgaccccta attttaatgg tggtgtgaac gttatgttct gggtgatttt catcctgatt
     9421 tttgtcggac tggcgttgca ggattccggt gcccggatga gtcgccaggc gcgcttcttg
     9481 cgggaaagtg ttgaagatca actcattctg gaaaaagcca aaggggaaga ggggctaacg
     9541 cgtgaacaaa ttgagtcccg tattgttgtt ccgcaccata ctattttcct gcagtttttc
     9601 tcactatata tcctgccggt tatttgtatt gctgcaggtt atgtgttctt ttctctgctt
     9661 gggtttattt aatcaaaagg ccgcacggag cggccattat cctcaaccca tatcatgctg
     9721 ctaataatct atccaatgac tgctgagcaa taaccaaatg ctgaccgcca aataatcttg
     9781 cacgatttaa cagcgtgtag agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta
     9841 gcggggatac tgactgatag ccgtcataga tttgtggcgg ctgttcagta tgcagcggta
     9901 acatcgctag gtcgcactct cggtcacccc agtagcaggc agggtcgaaa atgtacgggc
     9961 catccggacc cagcgcacag ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg
    10021 gttgatgcga ggccagacgc tgctgaatat gctcgacgat ggcgtcgata ttgccgaaag
    10081 cgatcccttt ctctgctgcc agttccaact gccagccaat ccgttgttca gcaaaaaacg
    10141 ttgaccagcg acgttgccag gtgttgggct gtggagttgt ggagagcgag ttatcaaaat
    10201 cgaggccaaa ttgtggttgg tcactccatt gatgtaaacg cgcaatttgc tgaccaagaa
    10261 taaatgcgct atgcgcatcc agcggacgat gtgggagata atccatcacc agaaaactgt
    10321 agtcacggtc agcgcctact gcccaaacct taggcacggt gacggtttta ctacgcgaca
    10381 gtaactccag ttggtcggct tcggcggtaa aaccgggaag cagttccctt tcatcacatt
    10441 tgacgaaaaa gtcatgtcct gcatagcgca aatgccatgc ggcatggact tctccgccag
    10501 gcagttcatt acgcagttcg atttcgcctt cacctaactg ctcgctcaaa agacgaatga
    10561 ttgcctgcca catgatgtct ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa
    10621 tgcggtgtaa aaacacgagc taacgcacac cacagactga ttattgccca aaaaccattt
    10681 attttctgtt gctattccat tcctcccagg tcgtgatggt gatttcagga tctttgccgg
    10741 ttgcactttg ggtaagcccc gaaaccagtt ctctaatttc ttcttcactt tgggtactga
    10801 taagtccaaa agtgttagtc cccagctcat ggatatttcc ctcatcatcc gtcatggtga
    10861 gcaaaaaacc gtcgcgagtc aggtaattat tcaactcgtt aatttcagtc aatgtatcct
    10921 catgcaacat gacggttatg acataacgga caagatcgcc actggccata gttcacccct
    10981 ttgttatcac atgacttttg agcatagtcg gagaaacgcg ttgccgacag gttggtggtg
    11041 attcccccaa tgctggggga atgtttttgt tagcgggaaa ggtaagcgta aattttttgc
    11101 gtatcgtcat gggagcacag acgtgttccc tgattgagtg tggctgcact ccccgcagct
    11161 acgccaaaac ggaccatctc ttcaagagag gcattttctg ccagtttcag tgtcatcgcg
    11221 ccgaccatgc tgtcaccagc gccaacggta ctctggcttt tcaccggtgg tggcaccacc
    11281 tgaatgcagt tttcactatc aacacccagc gctccttgtg gaccaaggga aacgacaacc
    11341 cgtttggcct tgccgctatt aacgatttcc tgcgcggctt tgcggacatc gtctggctgg
    11401 gtgagttcgc gattcaccag cgcactgagt tctttttggt taggcttaac caactcgatg
    11461 ttaccaatcg ccagtgctgc acttaacgct tcgccagaac tgtcgatgat gcagcggatc
    11521 ccttggtttt gcgcagcgga aatcagttgg gttaattttt ccagcttcac acctggcggc
    11581 aggcttccgc ttatgaccag gatggccccg gattcaattt ccagaacttg ctcttcaagc
    11641 tggcgaaact catcttcatt taatgccgcg cctggcataa caaaacgata ctgttcaccg
    11701 cttgcttcca catgtacgtg taaattctgc cgggtccagt ctttggcttc tacagtagcg
    11761 acggggacat tttcatccgc caacagtgaa accaggtgtt cgccggtcgc gccacccgcc
    11821 gggaagatcg ctgtggcact gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg
    11881 ccgcccccgg gttcgaacac cggtgcggta cagcgcagct ttccttcggg ataaatttgc
    11941 ggggtaattg ttgcgctatc gagagagggc gcaagtgtca acgtatagat acgtaccatc
    12001 atttcctcct ataggctgat ttcagtctgg caccgaatca gcggaaagtg aagaaattaa
    12061 caatatgatt tattagagct ttatttaaaa ttttgcagat aaatatatat aaataaaaat
    12121 ctgttttcgc cacatgctta ttcaaaaaat gaaataagaa tacattgcta agttattcgc
    12181 gcctttttat aattcgttac ctttccaggg acgctaatct ttgatcctta agaaagtttc
    12241 cgggaccgcg atggtctctt tttttgttgt acggactctt gataaatgaa gcttttgaag
    12301 acagttcctg ccatcgttat gttggcgggg ggcatgtttg cgtcactgaa tgcagctgcc
    12361 gatgattccg tttttactgt catggacgat cctgccagcg ctaaaaaacc tttcgaaggt
    12421 aatctgaacg caggatatct tgcacaatca ggcaatacga aaagctcctc acttacggct
    12481 gacaccacca tgacctggta tggacaaacc actgcctggt cgctgtgggg gaacgccagt
    12541 aatacttctt ctaatgatga gcgttcttca gaaaaatatg cggcgggcgg gcgtagccgc
    12601 tttaatttaa ctgattatga ctatttattt ggtcaggcaa gctggctgac agaccgttat
    12661 aacggctatc gagagcgcga tgtgttgacc gcgggttatg gtcgtcagtt tttgaatggc
    12721 ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc gttacgacaa atataccgac
    12781 aatgccagtg aaacccagcc gctgggctat gcctccgggg cttatgcatg gcagttgact
    12841 gacaacgcta aatttacgca gggtgtttcg gtctttggcg cggaagacac aacgctgaac
    12901 tccgaaagcg cattaaatgt tgctattaat gaacattttg gactgaaggt ggcttacaac
    12961 gtcacatgga actctgaacc tccagagtct gcaccggaac ataccgaccg tcggacaacc
    13021 ttatcgctgg gctacagcat gtaaaattat ctgccggacc gtgttatgcg gtctggctgt
    13081 cttcgtttgt cactgctcag ccccaaatca ttgctgccca acgtagcaac aacatagcgc
    13141 cacagattgc cagtagcacc accaccatct tccagtgttt tttatcaaag cgtttagtcg
    13201 gcataccagt gtcctgcata tctcttgtgc gtgataacgg agtgtaacaa aagggacagg
    13261 catcaaggga ttttttgtta ttacagccgt cgggtggcgt tgtgcacatg gtcactgttt
    13321 ctgcgtttta tgaaaaaaat aaaattatgt atcgatatat atcgtaaata gctggttata
    13381 aatacgtctt tatttcatca gcactaaaac tcaggctttg gctgatgaca tttatagcag
    13441 cataatatgt ttctacagtt ttataatgct tatgatgaat ggctatatga acagctgaca
    13501 tgttgtcatg atttttagcg gccaatagtg taaataactg atgttgacta aaagaatgtt
    13561 tttttgcaaa agcacccagc gtcgatatat aagataacac gacgtcttca ttacccttgc
    13621 tcatggcgat gtataatgca ggggttccct gtgctgtagc tccttttaat aaatccatga
    13681 tgttagcttt gctcaacttg tatttaaagg cgatgccatt aattttagag aggaaccgtg
    13741 tgacacacaa agggtgatta ttttccattg cggcgtataa tcctggctca ccatcatgta
    13801 ttttcatggc taaaatactc atcaccagtt ttttgtttaa aagctcttgt gctattggag
    13861 tggttaatgc attcagaaag atatcgatta ttt
//
LOCUS       DADKXM010000104        13869 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.105, whole genome shotgun sequence.
ACCESSION   DADKXM010000104 DADKXM010000000
VERSION     DADKXM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.105"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="JQR05_002942"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="JQR05_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY5325051.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="JQR05_002943"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="JQR05_002943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY5325052.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="JQR05_002944"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="JQR05_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY5325053.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /locus_tag="JQR05_002945"
     CDS             3241..4581
                     /locus_tag="JQR05_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY5325054.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /locus_tag="JQR05_002946"
     CDS             4659..6305
                     /locus_tag="JQR05_002946"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY5325055.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLEELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="JQR05_002947"
     CDS             6428..7057
                     /locus_tag="JQR05_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY5325056.1"
                     /translation="MIRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8605)
                     /gene="melB"
                     /locus_tag="JQR05_002948"
     CDS             complement(7196..8605)
                     /gene="melB"
                     /locus_tag="JQR05_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY5325057.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKLWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="JQR05_002949"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="JQR05_002949"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY5325058.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="JQR05_002950"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="JQR05_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY5325059.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="JQR05_002951"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="JQR05_002951"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY5325060.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDDAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3616 a         3604 c         3363 g         3286 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctgatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaagcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctccgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgga agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg atccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtagcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac cggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc tgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagagtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcctgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg atcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggaacatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gacgcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa cgccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccgcgct
//
LOCUS       DADKXM010000105        13695 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.106, whole genome shotgun sequence.
ACCESSION   DADKXM010000105 DADKXM010000000
VERSION     DADKXM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.106"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..172
                     /locus_tag="JQR05_002952"
     CDS             <1..172
                     /locus_tag="JQR05_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325061.1"
                     /translation="SLSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKIN
                     QTTTKMLFMCRE"
     gene            complement(280..681)
                     /locus_tag="JQR05_002953"
     CDS             complement(280..681)
                     /locus_tag="JQR05_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY5325062.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            923..1216
                     /locus_tag="JQR05_002954"
     CDS             923..1216
                     /locus_tag="JQR05_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325063.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1288..1947
                     /locus_tag="JQR05_002955"
     CDS             1288..1947
                     /locus_tag="JQR05_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY5325064.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2024..2485
                     /locus_tag="JQR05_002956"
     CDS             2024..2485
                     /locus_tag="JQR05_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY5325065.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2692..3592)
                     /gene="hlyE"
                     /locus_tag="JQR05_002957"
                     /pseudo
     CDS             complement(2692..3592)
                     /gene="hlyE"
                     /locus_tag="JQR05_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3965..4384
                     /gene="umuD"
                     /locus_tag="JQR05_002958"
     CDS             3965..4384
                     /gene="umuD"
                     /locus_tag="JQR05_002958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY5325066.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4384..5652
                     /locus_tag="JQR05_002959"
     CDS             4384..5652
                     /locus_tag="JQR05_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY5325067.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGRDIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMFSPRYTTRSSDLLRVK"
     gene            complement(5698..6228)
                     /gene="dsbB"
                     /locus_tag="JQR05_002960"
     CDS             complement(5698..6228)
                     /gene="dsbB"
                     /locus_tag="JQR05_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY5325068.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6374..7915)
                     /gene="nhaB"
                     /locus_tag="JQR05_002961"
     CDS             complement(6374..7915)
                     /gene="nhaB"
                     /locus_tag="JQR05_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY5325069.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8136..8855
                     /gene="fadR"
                     /locus_tag="JQR05_002962"
     CDS             8136..8855
                     /gene="fadR"
                     /locus_tag="JQR05_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY5325070.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8907..10439)
                     /locus_tag="JQR05_002963"
     CDS             complement(8907..10439)
                     /locus_tag="JQR05_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY5325071.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFLPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVQDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10769..12067
                     /locus_tag="JQR05_002964"
     CDS             10769..12067
                     /locus_tag="JQR05_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY5325072.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLVRMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFCDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12077..13147
                     /gene="dadX"
                     /locus_tag="JQR05_002965"
     CDS             12077..13147
                     /gene="dadX"
                     /locus_tag="JQR05_002965"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY5325073.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     LRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(13315..>13695)
                     /locus_tag="JQR05_002966"
     CDS             complement(13315..>13695)
                     /locus_tag="JQR05_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325074.1"
                     /translation="CNGNPWDKDNLPTLLKAVEGLKKQQGLKDLLDFLHKEGQVDLEG
                     LEELEDLMDLEFLDDPELLERVKVQEDLELLDQQLGLLSLERQQDSQPVNQQSEHEPE
                     SASRVKRDLSEVDSESTMKRKRFM"
BASE COUNT         3453 a         3247 c         3516 g         3479 t
ORIGIN      
        1 cagtctatca ttacaaggct acgatcccag cgaatttaca ttcgctaacg gtgtattttg
       61 cgatgttaaa gaaaaattat gtcgtgatga tcgttatttt ggtgtagatg gtaagcggag
      121 tggaaaaatc aatcaaacca ccacaaagat gttatttatg tgtcgtgagt gatgccttgt
      181 ttacattatg ttctctgcta ttcgtcatta acctcttgag cgacagagag gagtggggcg
      241 ttaacgtcta tgacggggat agtccccgtc ggttgacatt tacttaattt gaatatcgac
      301 gttatatttt ttagttttgt ttttacgggc gtcgtttcga gtttgcagca ctcgcagctc
      361 atatttgcca gaagcaggga gtgagtattg tccgtgacta tcgagttctg gggaatatct
      421 ggataaatca acagaatcat cgattcctgg cccgaagaga taagtatcag cgccttcatt
      481 agagatactc acatgcactt tctgaccttt tttggcatag aaggtatatg tatcgtaatc
      541 gtatcccttt atttcgccgg agtactgagc gctgctatgg cctttgctga actcaacatt
      601 gacattctta cccgcagcaa agctcgtgga ggttagcagt gcaagcagca atacagcctt
      661 actgatgctc ttgattttca ttggtagtct cctgttcaga atgagtacga tgaactgtta
      721 taatataaca atcccttacg ggagaattcc agtggctttt ctgcgttcgg taaaatcccg
      781 gcgcggcatt ttttgccggg gggattactt ataaaggggc atggactcaa caaaccgcag
      841 aaatctacag ctggctggct tgtggcgcgg gtttcattga tgttagctta tgccaaaacc
      901 tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca agcgtgacca
      961 gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gttcctgagg aactgatgaa
     1021 aggttttggt cagcctcagt tagcgatgat tctgccgttg gatgggcgga agaaactggt
     1081 caatgctgat attgaaaagg ttaaactggc attaaccgag caaggttact atttgcagtt
     1141 accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc agaaaacaga
     1201 cgacactaac aaataaccga tatccggcgg tggcattatc tttgtcggcg cgggttttca
     1261 tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca aggtgcgctg
     1321 ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa acatattaaa
     1381 gagatgggca gcgcagtgcc cgaagagcca gtgctgttta ttaaaccaga aacggcactg
     1441 tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca tcatgaagtc
     1501 gaactggcgg tgttgattgg cgcgacgctg cgtcaggcaa cggaagagca tgtccgcaaa
     1561 gccattgccg gttacggcgt ggcgcttgat ctgacattgc gtgatgttca gggaaaaatg
     1621 aagaaagccg ggcagccgtg ggaaaaggct aaagcgtttg ataactcttg tccgctttcc
     1681 gggtttatcc ccgcggcgga atttaccggc gatccacaaa atacaacgct gggcctgagc
     1741 gtaaacggcg aacaacgcca gcaaggtacg actgcggaca tgatccataa aatcgttccg
     1801 ttgatcgctt atatgagcaa gttttttacc ctcaaggccg gtgacgttgt gttgacaggc
     1861 acgcctgatg gcgtcggccc gttgcaaagc ggtgatgagc tgacagtcac tttcgatggg
     1921 cattctttga caactcgcgt tttgtaatac tttttgccgc cagaaagcgg cggcaaaact
     1981 tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg aacacctgat
     2041 gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg cggaatggga
     2101 gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa ctgatggatg aagacaccga
     2161 cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct gtcaatgtcg
     2221 gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt aaattaaccc gtgaaaatct
     2281 gccaacattc gaatggctgc caatgacctg cgcttatcgg ttgctggcgg aaggtaaaga
     2341 tttacctgcg tggcatccgc tacttactgg ttcgaaagcg gcaatgcatg gtgaacgtat
     2401 ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg atcatatctt
     2461 aaataaacct gactgggcgc agtgataaat ttaatagatg ctgatggggg ctttcttaat
     2521 atacgtctgg atgctcgcca tttcgtagcc ctgtttttga aagcgctctg tcataatgag
     2581 agttacccga taccttatta tcaacaggta actctcaatc tttaaaaaac gtctgtgttt
     2641 ttaaatgtga taaaccttat accttgagta catggagaga ataatcgctt atcagacttc
     2701 aggtacctca aagagtgtct ttttaccgtg tcttttctga tactcattac aggtgttaat
     2761 catttttttg gccgcttctt ttagcaaaga aagcattaaa tcatcataat caacatagaa
     2821 tctggttgtt tcagtttccg tttttatctc cccgatggcg gctatttcgg tggttaattt
     2881 caatttggcg gcatcgatat ctttattcgc ttgtttaacc gtgttagaca gggtggtaaa
     2941 gaaactctgc acagatttta acttgttctt caattctgga atcagtttcc cttcaactac
     3001 gcccgcagca atagaatagg aaatgattaa accaaatgga ccggcgacga caccggctgc
     3061 ggcaccggca tacgcttcct tcctgatttt atctacctgt gactggaaat agctgctttt
     3121 ttctgaaaaa tcattggtta actggctatc taacgccagc agtttcccgg aagcgttgtt
     3181 gaaactttgt gagcttacca gcagggattt ttgcgcttca ttcagcttcg tgatgccgtc
     3241 atccagtacc ttaatgaggg gcggatgctt tcttctcatt gtactcatca aatagcaaaa
     3301 tatacgctgc gagcaattgc gtcgcaacac cacaccattc atacactgtt tgggttgctt
     3361 caaaatactt attctggcta tccataagta aggttttaat atcgccgact aaaacggagg
     3421 ctgcctgtga atactcctgt ttaaagcgac ttaactcttt tatggtttca tcaaaggtct
     3481 gccaggggat gacctgatcg agatatttat tataaagatc taatgctcca tctgcggttt
     3541 cgattgcgtt tttaactact tctaccgttt tatctgcaac gatttcagtc ataatcattc
     3601 gcctctttaa atatataaat tgtaatgaaa ctcctgtttt acaactatta ataaatttta
     3661 cttcatctaa ttcatagtta gccgggcggg atgcgtcaat gtctttattt ctattaatat
     3721 gataaatatc aaacaatgtt taatgtcatt atggcgaatg cttctattct atttttttgc
     3781 cgggtgatat ttttcatttc tgctggatga gcgtcgtcgc cagaaggcca cgtgagcaca
     3841 ggataagaga acgaaaaatc agcagtctat gcagcgacaa atattgatag cctgaatcag
     3901 tattgatctg ctggcaagaa cagactactg tatataaaaa cagtataact tcaggcagat
     3961 tattatgttg tttatcaagc ctgcggatct ccgcgaaatt gtgacttttc cgctatttag
     4021 cgatcttgtt cagtgtggct ttccttcacc ggcagcagat tacgttgaac agcgcatcga
     4081 tctgaatcaa ctgttgatcc agcatcccag cgcgacttac ttcgtcaaag caagtggtga
     4141 ttctatgatt gatggtggaa ttagtgacgg tgatttactg attgtcgata gcgctattac
     4201 cgccagccat ggtgatattg tcatcgctgc tgttgacggc gagtttacgg tgaaaaaatt
     4261 gcaactacgc ccgacggtac agcttattcc catgaacagt gcgtactcgc ccattaccat
     4321 cagtagcgaa gatacgctgg atgtctttgg tgtggtgatc cacgtcgtta aggcgatgcg
     4381 ctgatgtttg ccctctgtga tgtaaacgcg ttttatgcca gctgtgagac ggtgtttcgc
     4441 cctgatttat ggggtaaacc ggtggttgtg ctatcgaata atgacggttg cgttatcgcc
     4501 cgaaacgctg aggcaaaggc gctgggcgtt aaaatgggcg atccctggtt caaacaaaaa
     4561 gatctgtttc gtcgctgtgg tgtggtttgc tttagcagca attatgagct ttacgcagac
     4621 atgagcaatc gggtgatgtc gacgctggaa gaactatcgc cccgcgtcga gatttacagt
     4681 attgatgaag cattctgcga tctgacaggt gtgcgtaatt gtcgcgatct gactgatttt
     4741 ggcagagata ttcgcgcaac ggtgctacaa cgtacccatc ttactgttgg tgtggggatc
     4801 gcccagacca aaacgttggc taagcttgcc aatcatgcgg caaaaaaatg gcagcggcag
     4861 acgggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat gtctgctctc
     4921 cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga cgcgatgggg
     4981 atcaaaaccg ttctcgattt ggcggataca gatatccggt ttatccgtaa acattttaat
     5041 gtcatgctcg aaagaacggt gcgtgaactg cgcggcgaac cctgtttgca actggaagag
     5101 tttgcaccga cgaagcagga aattatctgt tcccgctcgt ttggtgaacg catcacggat
     5161 tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga aaaacttcgc
     5221 agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc atttgcgctc
     5281 aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac tcaggacagc
     5341 agggatatca ttaacgctgc tacgcgatct ctggatgcca tctggcaagc gggccatcgc
     5401 taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc gcagctcaat
     5461 ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatggcggt aatggatact
     5521 ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat ccaacaacaa
     5581 tggcagatga agcgagctat gttttcacca cgttatacaa cgcgaagttc tgatttactg
     5641 cgggtcaaat aaatatagcg gcaggaaaaa agcgctcccg caggagcgcc gaatggatta
     5701 gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcacca ccagcaccgc
     5761 gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca tacctaaaaa
     5821 atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc
     5881 cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaatg gcgaaggata
     5941 gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca
     6001 gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc
     6061 acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa
     6121 catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa acgccatcaa
     6181 cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct
     6241 gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc
     6301 aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc
     6361 cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag
     6421 gggcaagcgt aaactcgacg cagagcaagc cgacgagtgt cagtacgagg gtgtaaggca
     6481 gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca
     6541 gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga ttggtaccag
     6601 tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg
     6661 ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca
     6721 gcagaccgtt gaaaatatag aacagcgaca gctgagcatg ttcagatgcc tgcaacacaa
     6781 actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca
     6841 ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata gcatgttcat
     6901 cggtgacacc ggtcaatgat gttgccagaa taatgactga caaaccaatc aagccaactt
     6961 ccgccagatg caacgccagc gcagtcacca gccagacgcc aataatcgcc tggacaatca
     7021 gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac tgttgcaaca
     7081 cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc ttctctacca
     7141 gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac atgcgcagga
     7201 agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca
     7261 ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc aggctacgca
     7321 gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt
     7381 gcaggtcggt gtcttcggta cgggaagagg ctacgcgatg ataaatacca taaaaaccga
     7441 ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg
     7501 ctgccgccac gcaaaaagag agcgacagca gcattttgga gcgaatgctt aacagcaaac
     7561 gggtaaatat gaacagcaac agctgtttca taaaatagat acccgccacc ataaacatca
     7621 ataacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca
     7681 tgccgatgaa taccgcttcg atagccaaca gaccaccggg gagtagcggg tagcatttca
     7741 gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccaacccgcg acgaaagggc
     7801 tgatgaggaa aattaacggg tttacgatta agaaaatgat gagggcgagt ttgtaccagt
     7861 cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc tccatgatgg
     7921 tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt
     7981 caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa aatgaagcct
     8041 tgatcccttt ttcttctttt tgtctgctat cagcgtagtt agccctctgg tatgatgagt
     8101 ccaactttgt tttgctgtgt tatggaaatc tcactatggt cattaaggcg caaagcccgg
     8161 cgggtttcgc ggaagagtac attattgaaa gtatctggaa taaccgcttc cctcccggga
     8221 ctattttgcc cgcagaacgt gaactttcag aattaattgg cgtaacgcgt actacgttac
     8281 gtgaagtgtt acagcgtctg gcacgagatg gctggttgac cattcaacat ggcaagccga
     8341 cgaaggtgaa taatttctgg gaaacttccg gtttaaatat ccttgaaaca ctggcgcgac
     8401 tggatcacga aagtgtgccg cagcttattg ataatttgct gtcggtgcgt accaatattt
     8461 ccactatttt tattcgcacc gcgtttcgtc agcatcccga taaagcgcag gaagtgctgg
     8521 ctaccgctaa tgaagtggcc gatcacgccg atgcctttgc cgagctggat tacaacatat
     8581 tccgcggcct ggcgtttgct tccggcaacc cgatttacgg tctgatcctt aacgggatga
     8641 aagggctgta tacgcgcatt ggtcgtcact atttcgccaa tccggaagcg cgcagtctgg
     8701 cgctgggctt ctaccacaaa ctgtcggcgt tgtgcagtga aggcgcgcac gatcaggtgt
     8761 acgaaacagt gcgtcgctat gggcatgaga gtggcgagat ttggcaccgg atgcagaaaa
     8821 atctgccggg tgatttagcc attcaggggc gataatccct tccgtttaaa gagcaaaccc
     8881 ctcaaacgag gggttttttg ttgtttttac agatttccca ttcttggcgg gcaacgttcc
     8941 agcaactcga tgctgccgtc ttcgttttgc tgttcgagca tcacatcaaa tccccacagg
     9001 cgatgcacat gcttcaggac ttctttgcgc ccccgatcca gcggtgcgcg attatgtgga
     9061 atataacgca gcgtcagcga acggtcgccg cgcaaatcca cgttccagat ctgaatattc
     9121 ggctccagat tacttaagtt atattgcgac gataaccggt tacggatctc ccgataacct
     9181 tcttcattat gaatagcgga aatctccaga taattatgcc gatcatcatc ctgcacggtg
     9241 aagaagcgga aatcacgcat cactttcggt gacagaaact ggctgataaa actctcatct
     9301 ttgaaatcac gcatcgcgaa atgcagcgtt tccagccagt cggaaccggc gatatccggg
     9361 aaccagtatt tgtcttcttc cgttggcgac tgacaaatcc gtttaatatc ctggaacatg
     9421 gcgaacccga gggcatacgg gttgatgccg ctgtaccacg ggctgttata ggggggcagg
     9481 aagaccacat tggtgtggct gtgcaaaaac tccagcataa aacgttccgt tactttccct
     9541 tcatcataca gatggttaag gatggtatag tgccagaagg tcgcccagcc ttcgttcatc
     9601 acctgagtct gtttttgcgg ataaaaatac tggctcacct tacgcacaat acgcaggatt
     9661 tcacgctgcc atgattccag cagtggcgca tttttctcca taaaatagag caggttttct
     9721 tgtggttcgg acggatagcg gcgcgcttca gcaaccgttt tctcttcctc gcgcttcggc
     9781 agggtacgcc agagcatatt gacctgactt tgtagatact cttcgcgact tttctgccgg
     9841 gctttctctt cttgcagcga gattttttgc gggcgtttgt accggtccac gccgtagttc
     9901 atcagcgcgt ggcacgagtc cagaagccgt tctacttcat caacgccata acgttcttcg
     9961 cactcggtaa tatatttacg ggcaaaaatc agataatcga caatcgaact ggcgtcggtc
    10021 caactgcgga ataagcaatt gtttttaaag aaagagttat gcccatagca ggcgtgtgcc
    10081 atcaccagcg cctgcatggt aatggtgttc tcttccatca agtaagcgat acacgggtta
    10141 gagttaatga cgatttcata ggccagccct tgctgaccgt gcttatacag ccgttcagtc
    10201 tcgataaact ttttaccgaa tgaccagtgc ggatagttaa ttggcatgcc gacgctggaa
    10261 taggcatcca tcatctgttc tgaggttatc acttcaatct ggtgcgggta ggtatccagc
    10321 cggtagagtt tcgccacccg gtctatctct gccagataaa catccagcag gtcgaacgtc
    10381 cagtcgggtc catcgctcaa acgtgtggtg tccttattca tagaatcgat cgtcgccata
    10441 cgcgcacctc attgttgtcg gcgctctctg tgtggagcac ctcatttcaa gcatagaaca
    10501 cctgttaaaa accgcgtcgc cggagaattt ttttctttgc gatttcttat tatcagagtg
    10561 ccactaatcc gcttctgaac ggaattttat gctggataaa aagggcgttc agcaggagat
    10621 actaaagacg ccatattgcc gcagagtcag ggagatgtga gtcagctcac cataaaaaag
    10681 ccgtatgttg aataatattt tcaactgagt tatcaagatg taattagatt attattcttt
    10741 tactgtatct accgttatcg gagtggctat gcgagttgtc atactgggaa gtggtgtggt
    10801 aggcgttgcc agcgcctggt acttaaatca ggcaggacat gaggtcaccg tcattgatcg
    10861 ggagccgggg gccgccctgg aaaccagtgc cgcgaatgcc gggcaaatct cccccggata
    10921 tgctgcaccg tgggcagcac caggtgtgcc tttaaaagcg attaaatgga tgttccagcg
    10981 ccatgcgccg ctggcggttc gtctcgacgg tacgcagttt cagttgaaat ggatgtggca
    11041 aatgttacgt aactgcgaca ccagccacta catggaaaac aaagggcgga tggtgcgtct
    11101 ggcggaatac agccgtgatt gcctgaaagc attgcgcgcc gaaaccaata ttcagtatga
    11161 agggcgtcag ggtgggacgc tgcaactgtt ccgtaccgaa caacagtatg aaaatgcgac
    11221 ccgcgatatc gccgtgctgg aagatgccgg cgtaccgtat cagctgctgg aatccagccg
    11281 cctggcggaa gtggatcccg cgctggcaga agtagcgcac aaactgacgg gcggcctgca
    11341 gttacccaat gatgaaaccg gagactgcca gctgtttacc cagaatctgg tgcggatggc
    11401 agagcaggcg ggggttaaat tccgctttaa tacgcccgtt gaccaactgc tttgcgacgg
    11461 cgagcaaatc tacggcgtga agttttgcga tgaagtgatt aaggccgatg cgtatgtgat
    11521 ggcgtttggt tcttactcga cggcgatgct caaaggcatt gttgatattc cggtgtatcc
    11581 gctgaaaggc tactcgctga ctattccaat tgcgcaggaa gatggtgcgc cagtatccac
    11641 cattcttgat gaaacctaca aaatcgccat tacccgtttc gataaccgca ttcgtgttgg
    11701 cggaatggcg gaaattgttg gttttaacac cgagctgttg caaccgcgtc gtgaaacgct
    11761 ggaaatggtg gttcgcgatc tctatccacg cggcggtcat gtcgagcagg cgactttctg
    11821 gacgggtttg cgcccgatga cgccagacgg tacgccggtt gtcgggcgta cacgctttaa
    11881 aaatctgtgg ctgaataccg gtcacggcac gctcggctgg acgatggctt gcggttccgg
    11941 tcagttgtta agcgatctgc tctctggtcg tacgccagcg atcccatatg aggatctaag
    12001 cgtagcgcgc tacagccgtg gatttacgcc atcacgtccg ggccatttac atggcgcaca
    12061 cagctaagga aacgagatga cccgtccgat acaggccagc ctcgatctgc aggcattaaa
    12121 acagaatctg tccattgtcc gccaggccgc gccgcacgcg cgcgtctggt cggtggtaaa
    12181 agcgaacgct tacgggcatg gtattgagcg tatctggagc gcgctcgggg ccaccgatgg
    12241 ctttgcatta cttaacctgg aagaggcaat aacgttacgt gagcgcggct ggaaggggcc
    12301 gatcctgatg ctggaaggat ttttccatgc tcaggatctg gagatttatg accagcaccg
    12361 cctgaccacc tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa
    12421 agcaccgttg gatatttatc ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc
    12481 cgatcgcgtg cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg gcgaaatgac
    12541 cctgatgtcg cattttgctg aagcggaaca tcctgatgga atttccggcg cgatggcgcg
    12601 tattgagcag gcggcggagg ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac
    12661 tctgtggcac ccggaagcgc attttgactg ggttcggcct ggcattattt tgtatggcgc
    12721 ttcgccgtcc ggtcagtggc gtgatatcgc caataccgga ttacgtccgg tgatgacgct
    12781 aagcagtgag attattggtg tccagacgct aaaagcgggc gagcgtgtgg gctacggcgg
    12841 tcgctatact gcgcgcgatg aacagcgaat cggcattgtc gccgcaggat acgccgacgg
    12901 ttatctgcgc catgcgccta ccggtactcc tgttttagtg gacggcgtgc gcaccatgac
    12961 ggtggggacc gtctcgatgg atatgctggc ggttgattta acgccttgcc cgcaggcggg
    13021 gattggtacg ccggttgagc tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc
    13081 tgccggaacg gtgggctatg agttgatgtg cgcgctggcg ttacgcgtcc cggttgtgac
    13141 agtgtaactt gttgtaagcc ggatcggagg cagcgtcttc tggttgcgaa aaaatcatcc
    13201 atccggctgg tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc
    13261 cattaaaaga atggacacta tttccccgga acctgaactc accgcacagg cgttctacat
    13321 aaaacgctta cgcttcattg ttgactcgga gtcgacttca gataaatcac gcttcaccct
    13381 tgatgcagat tcgggttcat gttcagattg ttgattaaca ggctgcgagt cctgttgtct
    13441 ttccagactc aacaggccca actgttgatc caggagctcc aggtcctcct gtaccttcac
    13501 gcgctccagg agttccgggt catccaggaa ctccagatcc ataaggtcct ccagctcctc
    13561 gagtccttcc aggtcaacct ggccttcctt gtgcaaaaag tccaaaaggt ccttcaggcc
    13621 ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc ggtaaattgt ctttgtccca
    13681 cgggttacca ttaca
//
LOCUS       DADKXM010000106        13633 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.107, whole genome shotgun sequence.
ACCESSION   DADKXM010000106 DADKXM010000000
VERSION     DADKXM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.107"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..68)
                     /locus_tag="JQR05_002967"
     CDS             complement(<1..68)
                     /locus_tag="JQR05_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325075.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRR"
     gene            76..576
                     /gene="tnpA"
                     /locus_tag="JQR05_002968"
     CDS             76..576
                     /gene="tnpA"
                     /locus_tag="JQR05_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY5325076.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(615..962)
                     /gene="grxD"
                     /locus_tag="JQR05_002969"
     CDS             complement(615..962)
                     /gene="grxD"
                     /locus_tag="JQR05_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY5325077.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1297..2124
                     /locus_tag="JQR05_002970"
     CDS             1297..2124
                     /locus_tag="JQR05_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY5325078.1"
                     /translation="MARINRISITLCALLFTTLHLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2252..2833
                     /gene="sodB"
                     /locus_tag="JQR05_002971"
     CDS             2252..2833
                     /gene="sodB"
                     /locus_tag="JQR05_002971"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY5325079.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2979..4148)
                     /locus_tag="JQR05_002972"
     CDS             complement(2979..4148)
                     /locus_tag="JQR05_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325080.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTPGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4314..4403)
                     /gene="ynhF"
                     /locus_tag="JQR05_002973"
     CDS             complement(4314..4403)
                     /gene="ynhF"
                     /locus_tag="JQR05_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY5325081.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4702..5727
                     /gene="purR"
                     /locus_tag="JQR05_002974"
     CDS             4702..5727
                     /gene="purR"
                     /locus_tag="JQR05_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY5325082.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5724..6656)
                     /locus_tag="JQR05_002975"
     CDS             complement(5724..6656)
                     /locus_tag="JQR05_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325083.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6769..7980
                     /locus_tag="JQR05_002976"
     CDS             6769..7980
                     /locus_tag="JQR05_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY5325084.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8271..9419
                     /gene="cfa"
                     /locus_tag="JQR05_002977"
     CDS             8271..9419
                     /gene="cfa"
                     /locus_tag="JQR05_002977"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY5325085.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9459..10100)
                     /gene="ribE"
                     /locus_tag="JQR05_002978"
     CDS             complement(9459..10100)
                     /gene="ribE"
                     /locus_tag="JQR05_002978"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY5325086.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10315..11688
                     /gene="mdtK"
                     /locus_tag="JQR05_002979"
     CDS             10315..11688
                     /gene="mdtK"
                     /locus_tag="JQR05_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY5325087.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11729..12985)
                     /locus_tag="JQR05_002980"
     CDS             complement(11729..12985)
                     /locus_tag="JQR05_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325088.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13293..13369
                     /locus_tag="JQR05_002981"
     tRNA            13293..13369
                     /locus_tag="JQR05_002981"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13327..13329,aa:Val,seq:gac)
     gene            13374..13450
                     /locus_tag="JQR05_002982"
     tRNA            13374..13450
                     /locus_tag="JQR05_002982"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13408..13410,aa:Val,seq:gac)
     gene            13558..>13633
                     /locus_tag="JQR05_002983"
     CDS             13558..>13633
                     /locus_tag="JQR05_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HAY5325089.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKP"
BASE COUNT         3299 a         3547 c         3249 g         3538 t
ORIGIN      
        1 cgcctcattt cacgctcctg ttgaccaccg ggtcttaact ggaatttaaa tgcctgtaat
       61 cgtttcacag gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga
      121 aggcattgtg ttttcctgat gcatgttcac ctggtctttg tcaccaaata ccgacgccag
      181 atttttgacc atgacacgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat
      241 tttgcagctg aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac
      301 tatcctccca aactggcgat atccagtctg gtaaacagcc tcaaaggcat atcgaatagg
      361 ttactgcgac gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg
      421 agtcctggct attttgccag tagctgcgaa ggtgcgccaa tatccgtcat ccgccaatac
      481 attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa
      541 ggacgaggtt ttacggcgca ccggataaag gactgacgga tgcccgttcg catccgtcag
      601 tattgcagga cggattattc cgcgtccggc tcttcagact tgtatttagc ggcagtttct
      661 ttgatcagct gctgcagttc gccacgctga tacatttcga tcacgatatc acaaccgccg
      721 accagctcgc cgtcaaccca cagttgcggg aaggtcggcc agttagcata tttcggcagt
      781 tccgcacgaa tgtccggatt ctgcagaata tcaacatagg caaaacgttc gccacatgcg
      841 gcaagcgcct ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc
      901 atgtacagca ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat agtggtgctc
      961 attgtcttgc ttcctcaaac ttctttacgg cagtaatctg acattgtagc gggtcagtgc
     1021 ggcatcggaa aataacattt ccatcacgct tttgctattt tatccctttg ctctatttgt
     1081 tgcatttcaa atattcgttt cttctctgat gcgcggcaaa ctggcgtatt tataaccatt
     1141 tttagctttc actgctgcat ttttttgcgc tcgccaacga aacgtatttt ttaacaataa
     1201 aagctattaa ctttctcttc ttctatgcat tagaatcatc aagttttgta aatcagacgc
     1261 aggcatgata gacctgcctt aacagaggga cgctcagtgg cgcggataaa ccgtatttcg
     1321 atcacgctct gtgctttact ttttaccacc ctgcatttaa cgcctatggc ccatgcttca
     1381 aagcaagcca gggagagttc tgctaccact catatcacca aaaaagcaga taaaaagaaa
     1441 agcacggcaa ccaccaaaaa gacccagaaa gccagccaga aaacagcgaa aaaagccgcc
     1501 agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc cattaccgct
     1561 tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc agccagcttt
     1621 accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc
     1681 gcgtcaggaa cccttgccga cgcgcacaaa gcgaaggtgc aaaaagccac gaaagtggca
     1741 atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggcggcag ctcaccgcgt
     1801 accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt gaaaattcgt
     1861 attccgcgta cggcgaatga aatgtatcac ctgcgtgatg caggacctat cgaacgtagt
     1921 gagctgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggtac agccgatcat
     1981 gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg tcaggaaatt
     2041 caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc tcgtcgggta
     2101 atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca gggtaagttc
     2161 atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac
     2221 gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata
     2281 tgctaaagat gctctggcac cgcacatttc tgcggaaact atcgagtatc actacggcaa
     2341 gcatcatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg
     2401 taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca
     2461 ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg gcgaaccgac
     2521 tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca
     2581 gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag
     2641 cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga ccaccgatgc
     2701 gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc
     2761 acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa
     2821 tctcgctgca taataactga tggcaaatgc agcattgcct gatgcgcttc gcgcgccatg
     2881 ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgtcagcaac
     2941 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttagc aacgcaaact
     3001 gtttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
     3061 gcccccgtca ccggcacaaa gctgtatccc agcccagcgg aaattaccgc accaccagca
     3121 gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct
     3181 ggcgcttcac tggcgacatg catcacgcgc atctgtaacg gaggtacaac cgcaaaggtt
     3241 gctgcgcccc acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg
     3301 attgccagca taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc gttaactgaa
     3361 cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc
     3421 agcattgccg tgacgaacac cggtgttgcg tgggtaatac tttgcagtac cggagagata
     3481 taggtgtaga gggtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc
     3541 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgtacca
     3601 cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc
     3661 agaaatgaca tccgccagcc gatggtttca cccaaccaag tcgccgccgg cacgccaccg
     3721 atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt
     3781 ggcaccacgc ttgcggccac gactgaaccc aaaccaaaaa atgctccgtg attcaggctg
     3841 gtcaaaatac gtgaaagcat cagggtcata taatccggcg cgatggcgga aagaacgttg
     3901 cctggcgtga aaattgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt
     3961 agaagcgtca tcagcggcgc gcccaccatt acgccaactg cataggcact gattaacatt
     4021 ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc
     4081 gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta
     4141 attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa
     4201 agcatgacag caatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt
     4261 tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc
     4321 agcgcggcag tcaaacccac ggctacgatc aaaccgagga cgataatcgt tgttaccagt
     4381 gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca caaaaagtga
     4441 tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt
     4501 ttttaccact tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt
     4561 actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt
     4621 tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gattttgcag gagctgaagt
     4681 tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt
     4741 tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga agaaacgcgc
     4801 aacgccgtgt gggcagcgat taaagaatta cactactccc ctagcgcggt ggcgcgtagc
     4861 ctgaaggtta accacaccaa gtctatcggt ttgttggcga ccagcagcga agcggcctat
     4921 tttgccgaga tcattgaagc agttgaaaaa aattgcttcc agaaaggtta caccctgatt
     4981 ctgggtaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa
     5041 aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg
     5101 ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac
     5161 ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg
     5221 attgaacgcg gtcaccgcga aatcggcgtc atccccggcc cgctggaacg taacaccggc
     5281 gcaggccgcc ttgccggttt tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa
     5341 agctggattg tgcagggtga ctttgaacct gaatccggtt atcgcgccat gcagcaaatc
     5401 ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc
     5461 gcactttgtg ctgctgatga aatgggcctg cgcgtcccgc aggatgtttc gctgatcggt
     5521 tatgataacg tgcgcaacgc gcgctatttt acgccggcgc tgaccacgat ccatcagcca
     5581 aaagattcgc tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt
     5641 gaagaaccgc agtccattga agtgcatccg cgtttgattg aacgccgctc cgtggctgac
     5701 ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc
     5761 agccactcct tattcagcgt ctcactatcg ccgagatact caagcagcca ggttaacgca
     5821 ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc
     5881 agttcaagtg ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc
     5941 atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc aggtacgaca
     6001 agtctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct
     6061 tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac
     6121 gccagcgggt ggtgactggc aacaacgcaa ctccagctta gcatccccat atcccggaag
     6181 gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg
     6241 tcggaaagcg catcccagac accgttgaac acttcctgaa agacaagaag ttcgacatca
     6301 tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac gttctggcct gacaatatta
     6361 tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg
     6421 gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc tccagcagcg
     6481 gtcagctcca catcacggtg ccgtcgttca aagagcggta ccgccagcca ctcttccagc
     6541 tgacgcacgg tatagctgac cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta
     6601 aaactaccat tacgcgctac cgcatcaaca acttcgagtg aatattctga ccacatagtc
     6661 tgcctgcaaa atttttgaaa ccagtcatca aatattaccg tttcacaaca ctaatttcac
     6721 tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg
     6781 aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg
     6841 tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt
     6901 gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc
     6961 tccgaccgtt atggtcgtaa accggtgtta ttaatcggcc tgacaatttt tgcgttaggt
     7021 agtctgggga tgctgtgggt agaaaacgcc gccacgctgc tggtattgcg ttttgtacag
     7081 gctgtggggg tctgcgccgc ggcggttatc tggcaagcat tagtgacaga ttattatcct
     7141 tcacagaaag tcaaccgtat ttttgcgacc atcatgccgc tggtgggtct gtctccggca
     7201 ctggctcccc tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc
     7261 accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg
     7321 aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc
     7381 tatcgcggca acgtgctgat atacgcagcc tgttcagcca gtttttttgc atggctgacc
     7441 ggttcaccgt tcatccttag tgaaatgggc tacagcccgg cagttattgg tttaagttat
     7501 gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc actgcagaaa
     7561 tggcaaggca agcagttatt accgtggttg ctggtgctgt ttgctgtcag cgtcattgcg
     7621 acctgggctg cgggcttcat tagccatgtg tcgctggtcg aaatcctgat cccattctgt
     7681 gtgatggcga ttgccaatgg cgcgatctac cctattgttg tcgcccaggc gctgcgtccc
     7741 ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc
     7801 ttcctcgcaa gtctggtagt ttcctggctt attagtatca gcacgccatt gctcaccacc
     7861 accagcgtga tgttatcaac agtagtgctg gtcgcgctgg gttacatgat gcaacgttgt
     7921 gaagaagttg actgcccgaa tcatggcaat gccgaagtcg ctcatagcga atcacactga
     7981 tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta
     8041 gcggcctata ctaatttcga gttgttaaag ctacgataaa tattatgttt ttacggggac
     8101 aggatcgttc ccgactcact atggatggtc atttcggcaa gggttcctcc tttccctctg
     8161 ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa
     8221 gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat
     8281 cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac
     8341 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc
     8401 ccgatttttt taaacgcgtt ctgcaggaag gctctttggg gttaggcgaa agttatatgg
     8461 atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc
     8521 tcgagaacca actcccccat catttcaaag acacgctgcg tattgccggc gctcgtctct
     8581 tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac gatttgggta
     8641 atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct tactggaaag
     8701 atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
     8761 agttaaaacc agggatgcgc gtactggata ttggctgcgg ctggggcgga ctggcgcact
     8821 acatggcatc taactatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa
     8881 aaatggctca ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg
     8941 acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgagcac gtcggaccga
     9001 aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc
     9061 tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat
     9121 atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcaatcc agcgaacccc
     9181 actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt
     9241 ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta
     9301 aacggatgtt tacctattat ctgaatgcct gcgcaggtgc tttccgcgcc cgtgatattc
     9361 agctctggca ggtggtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa
     9421 ggctattcta tcgccccctc ttcgggggcg agttcagatc aggcttctgt acctggttga
     9481 ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt
     9541 tgtggatcga tttcaatgtt gacgcgtgcg ccaagttttt tcttcccaag agtcgtgcgt
     9601 tccagtgttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc
     9661 aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta
     9721 tcctggactt taaaccagat ctggcgatta ttttccgagg ttaatatttt cgccacttca
     9781 gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcact gaatttcgcc
     9841 gcacgctcaa cgtttaccca atcccccact tttaaatcgc caagattggt aatgcgtaac
     9901 gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag
     9961 caaccgttat gcgccacgga agcaccggtt tccaggccgt ccagcatgtg gtcgggtaac
    10021 tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt tgcggtgccc
    10081 tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc
    10141 acaatagcag gtggaaaacg cccttaccag tgaaggggta agaatggcta ttttttcact
    10201 ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg
    10261 cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag
    10321 aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa
    10381 atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc
    10441 gacatggcgg cggtcgctat cggtacttct atctggcttc cggcgatcct ctttggtcac
    10501 ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag
    10561 cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt
    10621 atgctggtgc tgtggaatgc aggttacatt atccgctcca tggaaaacat cgatccggct
    10681 ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc gggatatctg
    10741 ttcttccagg ttgcccgtaa ccaatgtgaa ggtctggcaa aaaccaagcc gggtatggta
    10801 atgggcttta tcggcctgct ggtgaacatc ccggtgaact atatctttat ttatggtcat
    10861 ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg
    10921 gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc gctccatgcg cgatattcgt
    10981 aacgaaaaag gcaccgctaa gcccgatcct gcggttatga aacgactgat tcaactcggt
    11041 ttgccgattg cgctggcact gttctttgaa gtgacactgt ttgccgtcgt ggctctgtta
    11101 gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa ctttagttca
    11161 ctgatgtttg tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt
    11221 ctgggtcagg gctcaacgct ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt
    11281 gtctgtatgg caaccctgac ggccattttc acggtttcac tgcgggagca aatcgccctg
    11341 ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg
    11401 gtatatcaga tttctgactc aatccaggtg attggcagtg ggattttgcg tggttataaa
    11461 gatacgcgtt ccattttcta tatcaccttt acagcttact gggtgctggg cttgccaagc
    11521 ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg
    11581 ataggcttta ttatcggcct gacatcggca gccattatga tgatgctgcg tatgcggttc
    11641 ctgcaacgta tgccgtcagc catcattctg caacgagcgt cccgctaata aagacaaggc
    11701 gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt
    11761 cttcgtctgg ctgtaatttc agagcgttac acagagtttg ccgactggca tctgttatct
    11821 caacgcgtat atccccgtta acttcccctt tcacattgat gaagtcaggt gaaatttctt
    11881 tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact ttcagttgca
    11941 catgcgttgc cgcaccagtg atcagccaca gttttccgcc attcagattg atgttaccaa
    12001 caatgaaagt gacgctgtca ccttttaccg tactgccgtc gagaatcaac acaccgccct
    12061 gctgtacagt aatcgttgat tgttgcagct ctgcgccata accaataatc attgaagcgc
    12121 cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa
    12181 aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt attaccgcat
    12241 tttccagcgt accggcatgc acttcagccc gcccccccac attcaccgac aggttttgcg
    12301 tcttaccgga actgtagagc tgaaggccat ccgtccccag acgataaatg gatccattct
    12361 caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca gcaagcaacg
    12421 tatcactggc tttcccggcc agcataaaaa cgccgccctg attaatctca caattgctgg
    12481 cttctccact tggatacacc aacaattcac caccttcatc taccacgacc gctgaggttg
    12541 tcccattaac caacaggccg ccctcttgat cgagaatgat tttttccgcg cgatgtcctt
    12601 ccagtacacg caggttaccg ccattttcca gcaacaaacc gcaggcataa ccctgatcaa
    12661 cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag agactaattg
    12721 cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc gatagcgtgc
    12781 cattgttacc gtactgtgtg catttggcga tcccaccggt tgccactttc aaccagccag
    12841 cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac tcgccttcag
    12901 taagttgcgt cgcgccaatg acctcgccgg tcttaacaat ttgcacattc ccgtcgctca
    12961 tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg tgcctttatt
    13021 gctacctaag tgtaaaggct acggaggatt tatccacgac agatttgaga tggtggcaaa
    13081 caactctgtt taaactctga tacacgaatt attgggttgt atcagatgta aatgcgatcc
    13141 tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa aagctctgca
    13201 aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccacctttcc actctgccgc
    13261 taatattcgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg gttagagcac
    13321 caccttgaca tggtgggggt cgttggttcg agtccaattg aacgcaccat cctgcgtccg
    13381 tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc gagtccactc
    13441 ggacgcacca gattttctta atctggtctt ttcctttttc cctctgtttc ttctctgtat
    13501 ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagaaggag aatcgctatg
    13561 gcaactttgt tacaacttca ttttgctttt aatggcccat ttggcgacgc aatggctgag
    13621 cagcttaaac cac
//
LOCUS       DADKXM010000107        13593 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.108, whole genome shotgun sequence.
ACCESSION   DADKXM010000107 DADKXM010000000
VERSION     DADKXM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.108"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..897)
                     /locus_tag="JQR05_002984"
     CDS             complement(<1..897)
                     /locus_tag="JQR05_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325090.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1138..1938
                     /locus_tag="JQR05_002985"
     CDS             1138..1938
                     /locus_tag="JQR05_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY5325091.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2016..2786
                     /locus_tag="JQR05_002986"
     CDS             2016..2786
                     /locus_tag="JQR05_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5325092.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYFAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2834..4192)
                     /gene="mltD"
                     /locus_tag="JQR05_002987"
     CDS             complement(2834..4192)
                     /gene="mltD"
                     /locus_tag="JQR05_002987"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY5325093.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4264..5019)
                     /gene="gloB"
                     /locus_tag="JQR05_002988"
     CDS             complement(4264..5019)
                     /gene="gloB"
                     /locus_tag="JQR05_002988"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY5325094.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5053..5775
                     /locus_tag="JQR05_002989"
     CDS             5053..5775
                     /locus_tag="JQR05_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5325095.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5772..6239)
                     /gene="rnhA"
                     /locus_tag="JQR05_002990"
     CDS             complement(5772..6239)
                     /gene="rnhA"
                     /locus_tag="JQR05_002990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY5325096.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITLWLHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6304..7035
                     /gene="dnaQ"
                     /locus_tag="JQR05_002991"
     CDS             6304..7035
                     /gene="dnaQ"
                     /locus_tag="JQR05_002991"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY5325097.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7168..7244
                     /locus_tag="JQR05_002992"
     tRNA            7168..7244
                     /locus_tag="JQR05_002992"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7202..7204,aa:Asp,seq:gtc)
     gene            complement(7375..8421)
                     /locus_tag="JQR05_002993"
     CDS             complement(7375..8421)
                     /locus_tag="JQR05_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY5325098.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8432..9427)
                     /gene="tssG"
                     /locus_tag="JQR05_002994"
     CDS             complement(8432..9427)
                     /gene="tssG"
                     /locus_tag="JQR05_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY5325099.1"
                     /translation="MSNDFTQAQEPPWRYGFLNLMRRVDVQLCTVPAGNTWQPRMEKF
                     RLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGPLPLHYTELARNRTESRRD
                     PTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRFAFYVASLSGQDLRESADS
                     PLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQEYVLHWIAVADDEITRLGM
                     PAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFLPGSNNLPVLTELVRAFSG
                     YEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDNPVTGMIFEPEHYLAH"
     gene            complement(9424..11307)
                     /gene="tssF"
                     /locus_tag="JQR05_002995"
     CDS             complement(9424..11307)
                     /gene="tssF"
                     /locus_tag="JQR05_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY5325100.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11323..11817)
                     /gene="tssE"
                     /locus_tag="JQR05_002996"
     CDS             complement(11323..11817)
                     /gene="tssE"
                     /locus_tag="JQR05_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY5325101.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11814..12644)
                     /locus_tag="JQR05_002997"
     CDS             complement(11814..12644)
                     /locus_tag="JQR05_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY5325102.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEVITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHRAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12631..13545)
                     /gene="tagK"
                     /locus_tag="JQR05_002998"
                     /pseudo
     CDS             complement(12631..13545)
                     /gene="tagK"
                     /locus_tag="JQR05_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3113 a         3545 c         3627 g         3308 t
ORIGIN      
        1 tcctccggga gctgttttta catgctcgct aaggaaatcg ataaaagccc ggatgcgcgt
       61 acttaccgca cggtcgctgt aatagacggc actgaatggc atttccactg gcaacacttt
      121 atctgccatt aactccacca attctccgcg agcgatttct ttgtcgatca tgtagtcgga
      181 caaacacgca atcccgttgc cactcaggca aagctgtttc agtgtttccc cactattgga
      241 tgacaaaccg tacttcacct catgtaattg tccatcacta caggctatcg gccaggtatt
      301 gagggaagcg ggttcagtga atcccaggca aacatgttgc tttaaatcgt cgatcgtttc
      361 tggcttcccg tagcgggaaa tataatcggg ggaggcgata atttttcgat aactgttaaa
      421 taacggcctg gcacgtaagc tggagtccgt taacgtacca gcgcgtatcg cgacatccac
      481 ttttctttcg atcaaattaa taatcgtttc ggaggagact agcgataaag tgacttccgg
      541 atagcgttca cggaaaggct taattaacgg catcagaaag tgcagcacca ctggagttgc
      601 ggcatcgatc cgtaacagtc cacgcggtgt attacgcgtc tccataattt ctgattctgc
      661 cgctgccatc tcctgcaaaa ttgactgtac gcgacgaaaa taacgctcgc cttcttccgt
      721 caggctaagt tgccgcgtgg tccgattaag caggctaacg ccaagtttca tttccagctt
      781 tttcaccgcc cggcttaccg ctgagtttgc ttgccctaat tgttccgctg cccggctaaa
      841 gctgccgctt tcgacgaccg aaacaaaaat ggcgagttct tccgacgtgg ctttcatttt
      901 tgctcctgtt gcaaaataga tgagatattt tgaatttatt tgtcattaaa ccatcaggat
      961 gcgtgatatg tcatccgatt taatgttctc aataatgagc aaatttctga ccggtgtaag
     1021 cacttgctta cataacaata tacaattgct cgttgaaaga gtgagctaaa atccctataa
     1081 cagtagaacc ctcccgagtg cggaagggtt gacgtaatag aggtttcaaa gtcaaaagtg
     1141 cgaaaaaaca cctatgccat gcgctatgtt gccggacaac ctgcggaaag gatcttaccg
     1201 ccggggtctt ttgcgagcat cggccaggca ttaccacctg gagaaccgtt aagtaccgaa
     1261 gagcgtattc ggatcctggt gtggaacata tacaaacagc aacgcgctga atggttgtcg
     1321 gtattaaaga actacggcaa agatgcacat ctggtgttat tgcaggaagc gcagacaacg
     1381 ccagagttag tacagtttgc gaccgctaac tatcttgccg ccgatcaggt gcccgctttc
     1441 gtgctgccac aacatccttc cggtgtaatg accctttcgg cggcgcatcc agtgtattgc
     1501 tgcccgttac gtgaacgaga acccattttg cgtctggcga agtcggcact ggtgacggtc
     1561 tatccattac ctgacacccg cctgttgatg gtggttaata tacacgccgt caacttcagt
     1621 ctgggcgtgg atgtctatag taagcagtta cttcctattg gtgatcagat agctcaccac
     1681 agcggcccgg tcattatggc gggagatttc aatgcctgga gccgtagaag gatgaacgcg
     1741 ttatatcgct ttgcgcggga aatgtcgctg cgccaggtgc gttttaccga tgatcagcgc
     1801 cgtcgggcgt ttggccgccc gctcgatttt gttttctacc gtggtctgaa cgtcagcgaa
     1861 gcttctgtat tggttacgcg cgcttccgat cacaatccgc tactcgttga attcagtccc
     1921 ggcaagcctg ataaataagg tatgtcaggt ctgccacagg gcagaccaac gtttggcgct
     1981 gtccttttat actcaaacaa caaaaggaca gcgcaatgac aacacaatcc caccatgacc
     2041 acgtagaaaa gcagtttagc tctcaagcct gcgaatatct gaccagtacc gtacatgcat
     2101 ccggacgaga tttacagcgc ctggcggtgc gtctggctga ctatcctggt gcaagtgtac
     2161 ttgatatggg ctgcggagca gggcatgcca gctttgttgc tgcgcaaaac gtgagcgcgg
     2221 tggtggcgta tgacttatct gcccaaatgc tggatgtcgt ggcacaagct gccgaagccc
     2281 ggcaactgaa aaatatcacc acccgccagg gatatgccga aagtctgcca tttgccgata
     2341 acgcatttga tattgttatc agccgttatt ttgcccatca ctggcatgat gttggtgcag
     2401 cactgcgtga agtgaaccgg atattgaaac ctggcggtag gatgattgtg atggacgtaa
     2461 tgtctccggg tcatccagtg cgcgacatct ggttacagac ggtagaagca ttacgcgata
     2521 cctctcacgt acgaaactac gccagcggtg agtggttgag gttaatcaat gaagccaatc
     2581 tgatagttga taatttaatt acagataagt taccgctgga attttgttca tgggtcgcga
     2641 gaatgcgtac gccagaagcg ttagtagacg ctattcgcat ttaccaacag agcgcatcga
     2701 cagaggtgag aacgtatttt gccttgcaga atgacggctc tttcaccagt gatatcatca
     2761 tggtagaagc acataaagcg gcataaataa aaaaggcacc gggggaatcg gtaccttttt
     2821 attatctggt ttgtcaggaa tctggcatgt tgttgttttt cacaaacaac gtcagcttgt
     2881 cgcctggttg cagattcgca gtgtcgctgt tccagcgcat cacatctttg atgttcacgc
     2941 cgtggcgttt agcaatgctt gaaagcgaat cgcctttgcg cacacgatac gtaatgctat
     3001 cgctgttgtt tgccagtcgc tgtgcgctac tgcctgcacc aatcgtcaaa ctttggcctg
     3061 gcttcagctt agatccgcgc agtttgttcc actgctgcaa atctttggtg cttacgccga
     3121 gacgtgaagc gatgcttgaa agcgtgtcgc cagagcgtac ggtgtaaaca cggctgttaa
     3181 gcggcgtatt gtcggcaacc agcgtcgact gtacagcagc aatttcgcct gaagcgagag
     3241 attcacgcag ttgatctgca tgcttctttg gcaccatcac gtactgcggc ccacttgcgc
     3301 ccagcgtgga gcctttcacg ccagcgttga atgtcttcag cttgctgacg gaaatccccg
     3361 ccatatctgc aaccttcgcc atttcaaccg ggctactcag gtgcacacgc gccagagcac
     3421 ggctttcatc ggtcgttggc agacgtacgc cataacgctt gctgtttttg agaatatcac
     3481 tcaatgccag cattttaggc acgtactgct tcgtttcctg cggcaacggt aacgaccaga
     3541 agtccgtgga tttcccacgc gctttgttcg ttttaattgc cttcatgacc cgaccttcgc
     3601 cgctgttata agccgctacg gtcagaagcc agtcgccgtc aaacattttg ttcagacgct
     3661 gcatcatatt cagcgcggca gttgttgaag caacaacatc gcgacgcgcg tcataattgc
     3721 gggtctgttt caaaccataa ttgcgccccg tgctcggaat gatctgccag atgcctgcgg
     3781 cattggcgcc agacgttgcg tgaggatcaa aagcgctctc cactatgggt agtagtacca
     3841 gttccatagg catgttacgt tttttaactt gccctgctat ccagtacata tacggctctg
     3901 cccgtaaagt tacatcgtgg agatagctct tattgcgtaa atatttctgt ttctgttcgc
     3961 gaatccggtc attttccgga attcccatct ttagctcgtc gccaatgaaa gcccacaagt
     4021 caccatctgg cgcgatagac gtcccatcgt ccatccatcg tgcctgactt gtaaactttg
     4081 ctgcttcccc ttgaccagct gcagaaaggc tctgtgcgtg ctgttgaacg ttgccggtac
     4141 tctggcaacc cacgagcagg acagaggcga gtaatatcgc ttttgccttc atgtgtgtgt
     4201 caatagttgc ttaaaagacg accgatcata acggcgaacg gagccgatga caagaaagtt
     4261 ttatcagaac ctatctttct ttgaccttaa ccatgcaaaa cgctcttcag gttgttgcaa
     4321 taatgtttct tcattaatta cattgattaa atcaatatct tccgttctta aaaaaacatt
     4381 aatttgccgc tcatttttca gaattacggg tagtgttatt tgattttttg cccgtaactc
     4441 cttaacttta cgataataat catttatgga caaatcgtgc ggaaaaatac tcaaagcaaa
     4501 cttcatattt gataacgtat attcatgagc acaacatacc aatgtatcgt caggtaacgc
     4561 acttagcttt ttaagtgatt gatacatttg taaagctgtc ccttcaaaca accgaccaca
     4621 cccaccagaa aacagcgtgt cgccgcaaaa tagataaggt ttactgaagt aacagatatg
     4681 tcctaaagtg tgacccggtg tggtaattac actaaattca tgccctaaaa cgaaggcagt
     4741 ttcgccatct ttgactacct gtgttgttcc cttatcttgt gtctcttgtg gaccatacac
     4801 cacaatttgc ggaaactttt ccaccagttc ttttacgccg ccaacgtgat cgtggtgatg
     4861 gtgggttaga aatatggcct ccggttgcca gttattggcg gtaatggcgt ttaatactgg
     4921 ctctgcgtct ccgggatcga caatcaggca gcgacctgcc tcatcattca aaacccagat
     4981 gtaattgtca tcaaaggcgg gaatactgtt aagattcata gattacctct cagtgtaaaa
     5041 cggaaggttg tgatgaaacc ggcaagagtc cctcaaactg tcgtggctcc tgattgctgg
     5101 ggcgatttgc cctggggaga gctttatcgc aaggcgctgg agcgccagct caacccgtgg
     5161 ttcactaaaa tgtatggttt tcatctgctt aagattggca atttaagcgc agaaatcaat
     5221 tgcgaagcgt gcgcggtttc tcatcaagtg aatgtttctg cgcaaggaat gcccgtccag
     5281 gtgcaggcgg atccacttca tcttcctttt gccgataaat ccgttgatgt ttgtctactg
     5341 gcacatacct tgccgtggtg caccgatccg catcgtttat tacgtgaagc cgatcgggta
     5401 ctgattgatg atggctggct ggtgattagt ggcttcaacc ccatcagttt aatgggatta
     5461 cgcaaacttg tgccggtatt gcgcaaaacc tcgccctata atagccggat gtttactctg
     5521 atgcggcaac tggactggct ctctttgttg aattttgaag tgctgcacgc cagccgtttc
     5581 cacgttctcc cgtggaacaa acacggagga aaactattga atgcgcatat tcctgcgctt
     5641 ggttgcttac aacttattgt tgcccggaaa cggactattc ctttaacgct aaatccgatg
     5701 aaacagagta aaaacaagcc acgaattcgc caggcggttg gagccacccg gcaatgtcgt
     5761 aaaccacagg cttaaacttc gacttggtag cccgtatctt ccagtgtggg attcatcgcc
     5821 gcggcacgag ccagttcatc acagcgttcg ttttccgggt gtccggcatg gcctttaacc
     5881 cattcccatt tgatttgatg ctgccccaat gcagcatcaa gacgttgcca gagatcgaca
     5941 ttttttactg gttttttgtc tgctgttttc cagccacgtt ttttccagtt atggagccac
     6001 agggtgatac cctggcggac atactggctg tcggtactca aaatgacttc gcaatgttct
     6061 tttaacgcct ccagcgcgac aatagcggcc atcaactcca tacggttgtt ggtggtgcgg
     6121 gtgtagcctg cgctaaaggt tttctcgcgt ccgcgatagc gtaaaatagc gccgtaaccc
     6181 ccaggtccag gattacccag acacgaacca tcggtgaaaa tttctacctg tttaagcatc
     6241 tctggtagac ttcctgtaat tgaatcgaac tgtaaaacga caagtctgac ataaatgacc
     6301 gctatgagca ctgcaattac acgccagatc gttctcgata ccgaaaccac cggtatgaac
     6361 cagattggtg cacactatga aggccacaag atcattgaga ttggtgctgt tgaagtagtg
     6421 aaccgtcgcc tgacgggcaa taacttccat gtttatctca aacccgatcg gctggtggat
     6481 ccggaagcct ttggcgtaca tggtattgct gatgaatttt tgctcgataa gcccacgttt
     6541 gccgaagtag ccgatgagtt tatggactat atccgcggcg cggagttggt gatccataac
     6601 gcagcgttcg atataggctt tatggactac gagttttcgt tgcttaagcg cgatattccg
     6661 aagaccaata ccttctgtaa ggtcaccgat agccttgcgg tggcgaggaa aatgtttccc
     6721 ggtaaacgca acagcctcga tgcgctatgt gcccgctacg aaatagataa cagtaaacga
     6781 acgctgcacg gggcattact cgatgcccag atccttgcgg aagtttatct ggcgatgacc
     6841 ggtggtcaaa cgtcgatggc ttttgcgatg gaaggagaga cacagcagca acaaggtgaa
     6901 gcaacaattc agcgcattgt acgtcaggca agtaagttac gcgttgtttt tgcgacagat
     6961 gaagagctgg cagcacatga agcccgtctc gatctggtgc agaagaaagg cggaagctgc
     7021 ctctggcggg cataaataca tgtaaaaggc gctaaaaata gcgacttggg cgatttttgc
     7081 agcaaacgat tcaaaagatg agaaaaatcg ttgacgaagg tcgaggcaat ccgtaatatt
     7141 cgcctcgttc ccaacggaac acaacgcgga gcggtagttc agtcggttag aatacctgcc
     7201 tgtcacgcag ggggtcgcgg gttcgagtcc cgtccgttcc gccaatattc agaaagcccg
     7261 aatcagtaat gattcgggct ttcgtcgttt tggtgaagtg agaatttttc actccgaagt
     7321 aaaacggata tgatttaaaa aatggccaga cattataaat tctggccact tctgttactc
     7381 cagcgcatcc catttctcca gcagttctac tggaatttca tttattacct ccgagacgcg
     7441 ttttccgacc aggcgttctg cctggcgcag caaaggaatg gtcgggctgc tcggttcgct
     7501 gtgctcaaac cagcgacgga tgacacgcag gcgttccagc gcgtcgtttc ggtcgcggat
     7561 ctgccggggt tctgcgccgg tcatcggtga tatttcctcc gcgatatgca ccggtagcgc
     7621 aatctgctct gcctgcatct gttcagtatg aacgatggtg gcgttttccg gctgctcagc
     7681 ctgtggttgt ggcaggatct ccggcgtggt gctctgcact gctcccggta acagggccag
     7741 cagttgcgtc agacgggaga agtccggtgc ctggtcattc agcgtttcac gcgcatggcg
     7801 ttgcagccgc tcagtggtct ctgctgcctg gcgaaaggca tccagcggca gtgtgccgcg
     7861 cgcttccagg tctgccagtt gctggcgtac cgattctggt gccagcgcat ccgccgggcg
     7921 taatgcagat agcgaacgct ccacatcccg cacctgaagg cgcatggcag cattatttga
     7981 cagtgtaatg ccacgaatat ccgccattac accttcgtgg tccagcaacg ccgccagcgc
     8041 gttactgcgt gccaccgcag catcttcggc ggtgatatct tcagtcgcga gcagttgagg
     8101 atggatggcg tccgggtacg tggagcacaa cgtttccagc agcgtgagcg cctccgacag
     8161 tcccctggca ccggcctgct ggatacggca gcgcagcagg acaaccagaa tgcggatatc
     8221 tttgctgcgc atcagcagac gatgggcgtc ccgctcaagc tccgcccagt taatgttttc
     8281 cggcgtactg acaaaatcgc catattgcgc ttcttcacgc ggtgcggcgc gggtgaacag
     8341 cagcaggtat tccggatcgt actccagatc cgggccgcag gggcattccg ccgaaattgg
     8401 gtcagacagg aatatttcca tttaattatc ctcagtgcgc caggtagtgt tctggttcga
     8461 aaatcatgcc cgtcaccggg ttgtcgtgca tggctttacc cgcccatgca gtccaaccca
     8521 gttggtacgg accaccggta acagctggag gggcggcgtg gggctttagc tgtagctcaa
     8581 tttcccagca gtattcataa ccactaaagg cgcggaccag ttctgtcagg acgggcaggt
     8641 tattgctgcc aggcagaaaa cgtcggtaat cctccagcgt cagcgggccg ataaccagcc
     8701 ggaacttata ctgcatgtcc ggcacggcct ggccgatgag tgcaccattg ccaataacgg
     8761 aagagggggc aggcatcccc agccgggtga tctcatcgtc cgccacggct atccagtgca
     8821 ggacatactc ctgtatccgg aaaggtacac cgaaatagtg cgccagcgtg gtggccagac
     8881 cgtccggatt gcgggactca cgcaccagat gggccgatgc cgccagacga gcgtgatctg
     8941 gcaacgggct gtcagcgctc tcccgcaaat cctgcccgct gaggctggcg acataaaagg
     9001 caaagcggtc atgttgcggt ttatccagac cgcccccagc ggactgggcg ctgcgccatg
     9061 cctggtaaaa ctgcgtcagc cagcggtgat gaaaaagatc agaaaaacgg gtcagcgtcg
     9121 gatcgcgtcg gctttcagtc cggttacgcg ccagttcggt gtagtgcagc ggcagtggac
     9181 cgttcggccc ccacagcccg aggctgtaca gactgatgtg caggcgccct tcctgccagc
     9241 ccacactggc gatttctcgt ggcgcaaagg tcagcgcggg cgtttgtccg aggcgaaatt
     9301 tttccatccg tggttgccag gtgttgcccg ccggaacggt acacaactgc acatccacgc
     9361 ggcgcatcag gttcagaaaa ccgtaacgcc aggggggttc ctgcgcctga gtgaaatcgt
     9421 tgctcatacg cttccccgct gtcccgccct gacaggccag gtcataattt tgccgcgctg
     9481 catgctgtgc agcgtcatct gcgagaaggt attaatggaa acatggcggg cgatatagtg
     9541 ttccagcacc aggccgaaca gatacggact gatgccggaa aaaccttctt catccaccgt
     9601 cagttcacaa ctgacgccac gcccatagac cagtagtccg gaaccgggca gacggcgggt
     9661 gaccggggtc gttttacaac caatcaggct gcgcacctgg cgcgattgcg gactgtcatg
     9721 agcaggaata aacaggttca gcagatctcg caacgcctgg ccgccggtac ggtgatccag
     9781 atcggccagc ggcagataat taaaagataa ctggcggatc aaccgccatg ccatctcacg
     9841 ttcagccagc gggggctgcg gcgagcgtgg cgggcggata agccctacgc ccgccaccgg
     9901 gatggctgca tccacggtta gatcgtcccg gccattgcgg gcaataagac agggcagatc
     9961 gcggttagtg accatggccg tgatggtgat gtggcgcaga ttttccgggt acggcgcttc
    10021 atactggtca accagcgaca ggaacacttc cgagcccgta tagggggtac gggtgccgta
    10081 acggcgggcg ttttctgata agcggcgggg ctcccggcgc agggaaaaat aacgtccgtg
    10141 attgccttca tcattattgc gggtgtggta gagcgggcga aaggccatct tccgtgtggt
    10201 gtcggtttcc agcccttcca cttcctggac cgagaacacc tcataatcca gcggatgggt
    10261 tctgtccacc accagatgct gttccgtcgt gctgtgcgtg acatcgatcc gggcggtgac
    10321 gcggggaaac aggttgataa ccggggtaca gaacaggctg aactgtgccg catttgtctg
    10381 gtggatcagc cagtccggcg gcaggcggtt cagcaggata acaatctccg ccacgccacc
    10441 gtcaattttc tgtaatccgg cagacaggcc tgtcggggtg aaaaaataga agcgttccgg
    10501 acaggcaaaa tattcatgca gcaggttatg accgtgaaac acgttccacg ccagcggcag
    10561 cagcccctga tccggctcca ggccttcgta cataacgggc tgttgcagat tcacatccag
    10621 cgcgccgtca aaatgccccg gaatgccagc cagggtcgcg atggcgctgg tatgtagaag
    10681 ttctaacagg tgtgaggccg tacgctcctc gccgcagaga taaaacggca gcctgtccag
    10741 tccggcaagc tgactgaacg ttagttcacc aaaggtgcgc agggtaatac gcagcgcccc
    10801 ggcgacgtga atgttggccg gcaggtagcg gtgcagggca ggcatatccg gtggcgcggc
    10861 ggtcagtcgc gcctcttcga tggcgagcgg ccacagcgtt acatcctggc tgctgcgaaa
    10921 ctggcaggca gtgctttcgc cttccggaat ggcggagaaa aaggctgtgt cgcgcgggac
    10981 ggtaaatcct ttcgccagat cgccttcttc cgtatcggga tgcaactgcg ccacgctcat
    11041 tgagggcgtg ggtgtgacgt aattggggct gaccacttcc agcaggcgct gggtgaaacg
    11101 ggggaattca gcgtcaattt ttagctgcgt gcgggcggtc aggaagctga aggcctcgac
    11161 cattcgctct acatacggat cggcaatatc cgttccctgc atgcccagcc gggcggcgac
    11221 tttgggatga cgggcggcaa attcggctcc cgtttcccgc agatagctca gttcgcggtt
    11281 gtagtattcc agtagccgtg gatccatcgg tctctctctg aattaaaaaa acgtaatgcg
    11341 actctgctcc atatccagcg cgctacgtac ccggaatgca gtcggatacg gctgcgtgag
    11401 gatctcgccg cgaatttcaa actgtaatgt gttatggcca ctctggcggc gctcatcctg
    11461 aagtgggatg atttgcaggg tggacgcttt cagccggggt tcaaaccggg tgatggttcg
    11521 caaaatggcc ctgcggatat cgtcccactt gtgctcatac agaaagctgc ccgccagtgg
    11581 cggcaggccg tagttaagta cggacgctgc cgcgtacgga taacgtgcag catcaatatc
    11641 atcttcatgg ctgacggtgt tgagcagaaa tgacaaatcc cggcggatga tctccttaat
    11701 ctgaaccggg ctgacgctga taccgtggtc gcgtttctgg tttggggcgt cgtcacacag
    11761 gcggtcaaac agcgtgggca gcaggtgatt gcctggacgc tggcgggaag cgctcacgcg
    11821 tcgcttcctt cagcggtgtt aaaggtacag gtggacaaat ccagcaggct gatatcgccg
    11881 tgaccggtga gccacaccct ctgacccagc gcctgtacca gtgtctctcc cggaccgtcc
    11941 tgccagaccg tttcccggca cagacgcagg gagtcggcag ccttttcaga gccgctgtag
    12001 cggggaaaca gccaggcacg gtgtgtatcg ccattgttca gggtgacgtt gacgggtttc
    12061 cagatcaaat cgatcagatg cgcaggccgc ggcgatttca gcgaacagat ttgtgaaaac
    12121 ggcagccaga tatatgctcc gcccgcgatc agttcgagta ccggaccggt acgggagtcg
    12181 ctgtcgctaa tccagtcaaa tgcgccgcca ttccactgcc cggaagtgtc tgttatgacc
    12241 tcaagcgcgc ggctgcggtg agcatcagct tcctgcgctt caccacgggc gttacaggcg
    12301 agggcggtca gcagatcctc catccaggcg ggcggcggga gaataacacc gggccgctgt
    12361 tcgccctgaa agcaggcgtg gcggtatatc tcacagcgga tcagctcgcc aaatacctgc
    12421 gcttcgcgag tataatttgc gtccatccgg gcgcatagct ggatctgttg cagtgcccgc
    12481 gaccagtcgc ctgccacgca caataactga aacaggctgt ggcggcggga ggcctgcgct
    12541 gggtgcatcc tgacctgttg ctctgccatc tgaatgccct ccaccacgga ataatcccgc
    12601 agcagcgccg acaaggtaat cggtagctcg ttattgtttg tcatgatata tctctccgtg
    12661 ttgtgccggg tttatccggt aatggctgtc aatgccaatg atgcgatgct ctttccgtgt
    12721 cagatctggc agtacggtgt tatgcgctgt ttccggggcg tattccgggg aaagtaaatg
    12781 caggatatca gggggagtct tttcgacagg ccagatagtt tcatcttcgc cagtggcatc
    12841 cagtacattg agaatagcgt caatgcttaa cccaccagtg accatatcct gcaatgtctc
    12901 ggcttcggtg accaggtcac gcaatgaatc cagatcctgc gaggagggct cttcgttaaa
    12961 aggaaaagga tcagcatggc gattaagatc ttgttcggga gataacaacg cctcgcggta
    13021 ttcctgataa agtcgggtca gggcatttcc tttttccgca ccttctccgg tggaggaggc
    13081 tccatgaaca ggaatgatat cgaatggatt ttgtgatgtc agatgctgct gcctgaacca
    13141 atccagatcc agatattcgg ttattgcttc cgacgtgatg gcagtctgcg atatcgtttt
    13201 tactgtgctc aggtccggag atgatggatt gccatgtaca aggcactatg aagataaccc
    13261 ccattctatc aggtcgcctt cattcagacg catacggtga tgaggttcca caatctggtc
    13321 gtttaccgta cagcaatatt cggtagactg attgataatc caccatgctt cttcgtggcg
    13381 ggtcagagtg agggcaatct ttacatcatc attcacctta aaaacaggaa tccagggaga
    13441 ggtaacggtg aagataattt gtgttccttc agtcgcgtaa attgtgttat cggcgatccc
    13501 gtgcgcctgt attttttgga gtccccacgt aatatttggt ttcattgtgt atagactggc
    13561 cccctgaatc tccagacaac cagtatcact taa
//
LOCUS       DADKXM010000108        13454 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.109, whole genome shotgun sequence.
ACCESSION   DADKXM010000108 DADKXM010000000
VERSION     DADKXM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.109"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQR05_002999"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQR05_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY5325103.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JQR05_003000"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JQR05_003000"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY5325104.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JQR05_003001"
     CDS             complement(3736..4092)
                     /locus_tag="JQR05_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY5325105.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKNSLELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JQR05_003002"
     CDS             complement(4096..4512)
                     /locus_tag="JQR05_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY5325106.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JQR05_003003"
     CDS             complement(4623..5336)
                     /locus_tag="JQR05_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY5325107.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JQR05_003004"
     CDS             5699..6241
                     /locus_tag="JQR05_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325108.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JQR05_003005"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JQR05_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY5325109.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQCVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JQR05_003006"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JQR05_003006"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY5325110.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRGAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JQR05_003007"
     CDS             complement(9042..10457)
                     /locus_tag="JQR05_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY5325111.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JQR05_003008"
     CDS             10540..11523
                     /locus_tag="JQR05_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY5325112.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JQR05_003009"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JQR05_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY5325113.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JQR05_003010"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JQR05_003010"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY5325114.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSQFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFLGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            13191..>13454
                     /locus_tag="JQR05_003011"
     CDS             13191..>13454
                     /locus_tag="JQR05_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325115.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKA"
BASE COUNT         3400 a         3475 c         3329 g         3250 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat tccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct tgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ctcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc caggctgttt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccactc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttacacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacattgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcactag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gccccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caggctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataagtgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccggg cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgagataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccca
    12181 ggaacaagcc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggaa agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacgctaa
    13021 actgagaatt ccagtaaaca ccagtttttt caggcatcac gctggtttga ttaatttttt
    13081 gtgtttcatg attatcgtgc atttttgaac atttcaggct atttttctcg cgtcaggttc
    13141 ccgcacaggt tcccacgttt tatgggaacc cgaaataacg aggtcgtgta atggcgtact
    13201 ataacataga gaaacgacta aaatccgatg gcacaccacg ctatcgctgt aatgtgatta
    13261 tcaaagaaaa aggtgttatc acttacaggg aaagcaaaac attccctaaa catgctcatg
    13321 ccaaaacatg gggcacacag aaagtgatgg aattagatct atatggaatt ccatcatcaa
    13381 atgcagttga cggacttaca gtccgtgact tactacacaa atatttaaat gacccaaatg
    13441 ccggaggtaa agca
//
LOCUS       DADKXM010000109        13346 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.110, whole genome shotgun sequence.
ACCESSION   DADKXM010000109 DADKXM010000000
VERSION     DADKXM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.110"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            228..824
                     /locus_tag="JQR05_003012"
     CDS             228..824
                     /locus_tag="JQR05_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY5325116.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPRVLGDAAAKFFATDTYINGTGGVVERDICNFPKREACLMAMSYS
                     YELQAQVYDHMTELEGGKDINLLDFSGLTDMAISEMQNRVAAAEKFSFEMHGQAGSAL
                     MTRRKKEKKAIKKAEQLVKDLIQFKLCDMGDFPDGKPA"
     gene            1157..1711
                     /locus_tag="JQR05_003013"
     CDS             1157..1711
                     /locus_tag="JQR05_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY5325117.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEDSAAVQGCKNLI
                     KANIAKRIAELKAERNEEVGIDAAYVLRRLVEIDQMDVLDILLQNGELKPIKDWPKVW
                     RTTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITKLTPEQAAEVYRKMMG"
     gene            1714..3333
                     /locus_tag="JQR05_003014"
                     /pseudo
     CDS             1714..3333
                     /locus_tag="JQR05_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
     gene            3333..4874
                     /locus_tag="JQR05_003015"
     CDS             3333..4874
                     /locus_tag="JQR05_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY5325118.1"
                     /translation="MWPFKRKEPSAPAVPDKQPEPQPPEINDEVIASVRQKPRREFVR
                     YEPPPGVIPAPVRDAVLAMDATPYDTLNSHYPDFVYGGFPGYPYLALQAQLPEYRRMV
                     SVLAEEMTRKWIKVKAVGEGDDSHASRIAQLTDALEHYNVQDVFRLAIEHDGFFGRGQ
                     IYIDVRSPSGISAWTDPAELGSGLFISDKKIPKGSLLGLRVIEPVWTYPGIYNADNPL
                     SDDFYRPSEWYVMGKTVHASRMIDLISRPVPDMLKPAYNFGGLLLVQIAEPYVDNWLR
                     TRDSVGDMLHSFSLSGIKTDMTQVLSGKRDPNYAKRAELFNRTRDNRGLLMLDKTNEE
                     FFQFNTPLSGLDTLQAQAQEHMFFVSAIPSVKFAGLSPTGLNASSEGEIRVFYDTIAA
                     LATRLLKKPLKKVLDIIQLSEFGDIDPDITFEFEPLHELTREQLANIRKTEAETDQIY
                     ESTGAVTNNEVRERLATAQDSPYSGIDLSGDIEIDDTEIDNTEENPPQGPNADPEADF
                     TQRGD"
     gene            4915..5604
                     /locus_tag="JQR05_003016"
     CDS             4915..5604
                     /locus_tag="JQR05_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY5325119.1"
                     /translation="MHNSVLYWLRAEYRKTDLAQDASPVNLMRGAMQQLARRWQKNFD
                     EMALRLARRFAGDVLKNSDASLYTALRDAGFTVPFRMTTEMNTALQASITENVNLIRS
                     IPQQHLPQVETLVMQSVGRGRDLKTLTDELEKRYGITRRRAALIARDQNNKATSVMQA
                     ARQRSVGITEGVWRHSRAGKTWRPSHVKANGKRFDLRKGMFLDGKWVLPGEEINCRCG
                     WEAVIPGLEKK"
     gene            5601..6950
                     /locus_tag="JQR05_003017"
     CDS             5601..6950
                     /locus_tag="JQR05_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY5325120.1"
                     /translation="MIITEMLAFDRASVRQFDKVGRLQIERSNLSKANVCGYFGYEIP
                     GAEALGLDPKKLYQLYRDPDELRKAVSTFNNIPVLCRHKPDYPGAPAREYRVGTTHAN
                     SEFDGTYLVNGMSIWDNSAIAGIETDEQREISSSYAYVADMTPGTTPDGELYDGVMRN
                     IVGNHVALVGDGRVGSDCLVMDSLPQELKRMKLSKKEVAVLTALGTYLAPRLAQDAAP
                     GDLLRLMAQYKRPAAIASAVKTAYSERLVQDMDIEPVELAQLMESAEAVPELVGDDDT
                     GLTDESKAFDTDSPMESVLALLSGKVPDDVLEKIKSALSPTTDEAPETKEADVKPDDV
                     KVDKPAMDAAIRLATDQATKRAAENFRAVRVAEAEVRPLIGDVVAMDCAEDVYRTALE
                     QTGIDIQGIHPGAYRSMVKFAVEQKQTAKSSPRVAMDQASASTFAADFPGAKLKRGY"
     gene            6952..7434
                     /locus_tag="JQR05_003018"
     CDS             6952..7434
                     /locus_tag="JQR05_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325121.1"
                     /translation="MNTFQTHMNQYPAPGIPGAFASANPHASYVAGDGGLITGPDGLV
                     IARFAWVTKGVAANKGAGAPAGFVPRDGQASIVEWLAGDSNTIYPGRECTLMVSGDFW
                     ALTTTTATIVQKVFASLATGEIATGAAGTSMAGFVETWFSVASAAAAKEVIKISTWSK
                     "
     gene            7434..8462
                     /locus_tag="JQR05_003019"
     CDS             7434..8462
                     /locus_tag="JQR05_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5325122.1"
                     /translation="MNKFKQHYATASRDYGIILPGAQAYLPKEYAADYGLAMDAQPAL
                     VTAANSGIPAYFTNYVEPELIRVLVTPMKAAQILGETKKGDWTTLSAQFPIAESDGEV
                     SSYGDYSNNGVVSANVNWVPRQSYHFQTFTVWGERELEMYGAARIGWAAELNVASALT
                     LNKFQNKSYFYGIAGLTNYGLLNDPSLSAPITPDTVDGKLKWDDKDGQGVYDDIVKLF
                     KQLVKQTNGHIERTDKMKLCMSPLAEVNLTKTNQYRVNVSDLLAKNFPAMTIETAVEY
                     TSDAGELVQLIAERLGEQDTGYCAFTEKMRAHAVVADTSSWKQKKSAGTWGAIIRQPL
                     AYAQMLGV"
     gene            8466..8813
                     /locus_tag="JQR05_003020"
     CDS             8466..8813
                     /locus_tag="JQR05_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325123.1"
                     /translation="MAEMVTVGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQ
                     VDKVFFDAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPG
                     VNRNDEVMGKPQE"
     gene            8819..9277
                     /locus_tag="JQR05_003021"
     CDS             8819..9277
                     /locus_tag="JQR05_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY5325124.1"
                     /translation="MGTVTFDCRMFVSLYPEFSMVTPELLTAVFNQATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGVDGK"
     gene            9267..9851
                     /locus_tag="JQR05_003022"
     CDS             9267..9851
                     /locus_tag="JQR05_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325125.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPP
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            9848..10213
                     /locus_tag="JQR05_003023"
     CDS             9848..10213
                     /locus_tag="JQR05_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325126.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRVQKQAVTQADLRHLDNLNQQGVFATLYTDGNWCGLNRAWQQGGDKFVIGDETWLV
                     IEVPEIWPDWTRVIVCLQV"
     gene            10198..10743
                     /locus_tag="JQR05_003024"
     CDS             10198..10743
                     /locus_tag="JQR05_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325127.1"
                     /translation="MPSGVTLSVTESALYQALGDYLRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            10724..12208
                     /locus_tag="JQR05_003025"
     CDS             10724..12208
                     /locus_tag="JQR05_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY5325128.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTLMLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTTQ
                     NAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAAS
                     QQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTEHIAYQIIAV
                     NNYSSVVPVFATDGNRAAAVLGYAACLDFVRPEGRVSFKFREYEGLAADVTSGSDYDA
                     LIAAGYNFYGKYAENSVVEDYWADGTITGDFKWLDSFCGQIWLNASLQGAVIALFKSN
                     QTIPYNNEGRALVAASMSDVIQQYKRWGGIREGVTLTEAQKKQINNVVGADVSSTLFA
                     TGYYLYIGDMLPSLRAPRSSPSCTLWYCDGGSIQKLVIASTEVQ"
     gene            12209..12655
                     /locus_tag="JQR05_003026"
     CDS             12209..12655
                     /locus_tag="JQR05_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325129.1"
                     /translation="MSGNNNTITAADAIITLTVNNLYPSGVRLQGFATDNVYGTDPLV
                     LAETARGIDGKLSAGFVYSNIIQTFHIMPDSPSRDIFDTWSTTSRASRAVFRCNAVVL
                     LPAIGRKYTCVNGVLKQWKALPDAARTLQPGQAVIEWETITPEVFN"
     gene            12655..13059
                     /locus_tag="JQR05_003027"
     CDS             12655..13059
                     /locus_tag="JQR05_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325130.1"
                     /translation="MARKEKFITIEGQGRDKGKVFHLTEMSASQAEWWAMRAIMAMGR
                     GGVELPDDVRSLGMAALALEGLKALSKIPPDEARPLMDEMMDCVQFVPDPKKRSIRRP
                     LVEEDIEEIITRFNLRAEVFKLHVDFFATVVS"
     gene            13101..13283
                     /locus_tag="JQR05_003028"
     CDS             13101..13283
                     /locus_tag="JQR05_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY5325131.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT         3262 a         3193 c         3834 g         3057 t
ORIGIN      
        1 catcagggct cagtgcctga aataaatttt tttgcaaatc acaaagtcca tttaatgagc
       61 ctcgcacttg cggggctttt atatgtccgc agtaaaccgc gcatcgcagc gcgtaacaat
      121 cccgagtttt tcagaaagct gagcctgaga actgccgtat aggtgaggac ctctcgggga
      181 cggcttttct gtgcgaacag gctcaacttt ctaaaggaaa taccgacatg aacaaatcat
      241 taaccgttct tccttctggc gaatacccga ccatgagcag tcttgagatg gtggattaca
      301 ttaatgctga tcggaaatcc aaagcggagg cggaggggct tgcgttcccc tgtaagaaat
      361 atcgcaaact cgaacaccgc agtttcatga agaaagtacc cagggtcctc ggggatgcag
      421 ctgcaaaatt ttttgcaact gatacctata tcaacggaac aggtggtgtt gtggagaggg
      481 atatttgcaa ttttcccaag cgtgaagctt gcctcatggc aatgagctac agctatgagc
      541 ttcaggcgca ggtgtatgac cacatgacgg agcttgaggg cgggaaggac attaacctcc
      601 tcgatttctc tggcctgacc gatatggcaa tcagcgaaat gcaaaaccgt gtcgcggctg
      661 ctgagaagtt ctcatttgaa atgcacggtc aagcaggtag cgctctcatg actcgtcgga
      721 agaaagagaa gaaggccatt aaaaaggctg agcagcttgt gaaggatctg attcagttca
      781 agctatgtga catgggggat ttccctgatg gtaaaccagc atgactccga ttgatttcat
      841 acataaaaat gtaacaaccg agttaataaa gcttgggtac gaccagaacg cggctatgac
      901 tggagctgac atggcggtgg agcattaccg ccgttgttca caagccagca gaaaaggccg
      961 agttttcgat gactgcctgt acattgcaaa acagtgggca ggtaggcaga aaagggaaaa
     1021 aatgacaacc tgagagctac tttcacaacg gctcttcatt acaaagccta tctacgggtg
     1081 ggcttgataa tggcttatac cctacacggg ataacttaac tgatatccct tttaacggat
     1141 aaacggagca caaataatgg caaagctcac cgacaaacaa gagctgtttg cccgtgagta
     1201 cctgaaagat ctcaatgcca cgcaagcagc catcagggcg ggttacagtg aggattctgc
     1261 cgccgtacaa ggatgcaaaa acctaataaa agctaatatc gcaaagcgca ttgctgagct
     1321 taaagccgag cgaaacgaag aggtaggtat agatgccgct tatgttcttc gtagattggt
     1381 tgaaatcgac cagatggatg tgctcgacat tctcctgcaa aacggtgagc taaagcccat
     1441 taaagactgg cctaaggtat ggcgcacaac gctatcagga atggatgtcg tggagatggt
     1501 atccgcagat agcgccgcac ttctgaagaa aatcaaatgg cctgataagg ttaaaaacct
     1561 tgagttgctt gggcgccatg tttctgttca ggcgtttaaa gacaacgtca aaaatgaagt
     1621 gactggcgct gacggaggac cagtcagaac agaaattacc aaattaacgc ctgagcaggc
     1681 cgcagaggtg tatagaaaaa tgatgggcta agtatgccgt taccattccc cttcgatttt
     1741 aaacatcctg attaccagat ggtttttgaa tggcggatgg aacgcctaca gcgcattcgc
     1801 cagaaccctg aaatattgcc cgtattgaag cagttttacc gaaccaatcc ggctcagttc
     1861 atcatcgact ggggcatgac aacggacccg cgtaatattg attatggcct gccggtgacc
     1921 attccgtttt tactcttccc taagcaggag gagtggatcc actggattat ggaacgctgg
     1981 agcaatcggg agaatggtat taccgaaaaa tcccgtgaaa tggggctcag ttggaccgcg
     2041 atcggactgg cctgctcgct ttgtctcttc aacaaagaaa tggttatcgg tttcggctcc
     2101 cgtaaagagg aatacgtcga cagcaccggt gacccgaaag cattgttctg gaaggcgcgc
     2161 aagttcgtgg aaactctacc tgtagagttt cgcggttcgt ggagcgagaa gaagcacgcg
     2221 ccatatatgc gtgttgagtt tcctgaaact ggcgcggtta tcaaaggtga ggctggcgat
     2281 aatatcggtc gtggtgaccg tacgaccctt tattttgtgg atgaggctgc ttttctccag
     2341 cgaccattac ttattgatgc cgcgctttcc cagacaaccc gatgtcgtat cgatctctca
     2401 tcggttaacg gcatgaacaa ccccttcgca cagaagcggc acagtggaaa aatcccggtg
     2461 tttacgtttc actggcgtag cgatccgcgt aaagacgatg agtggtatca caaggagtgc
     2521 gagaaaattg ataacccagt catcgttgcc taggaactgg atcttaatta ccaggcatca
     2581 gcagaaggca tcctgatccc ctcagaatgg gtacaggctg cggttgatgc acatatcaga
     2641 ctgggcattc agcccggcgg ccaacggctt ggtgcaatgg atgtcgccga cgaagggcgg
     2701 gataaaaacg cctgttcgct ccgttatggc atcctgctga atgacgtaca ggagtggtca
     2761 ggtaagggga gtgacatcta tgactctgtg gttaaggttt ttggtctgtg cgatgacttt
     2821 ggtgccgatg agtttcgctt tgatgaggac ggattgggtg ctggcgttcg tggtgatgcg
     2881 cgcgctatca acgaactgcg tgaagcggag ggcatctgtc aaatcaccgc cactccattc
     2941 cggggcagcg ggagcgtgtt tcatcctgaa aatgaagctg ttcccggtga taatggtaaa
     3001 ccggctcgtc tgaataagga cttttttgtc aatgccaaag cccaggggtg gtggcatctt
     3061 cgcaaattat tccgcaatac atttcgtgca ctacagggta tggagtatga cccggatgag
     3121 attatttcca tcagcagcac aatggaaaat aaagaccggt tgttgatgga gctgtcacaa
     3181 cctacctggt caaaaaatgc caccggaaaa attcttgtgg ataagcaacc tgacggaacg
     3241 aaatctccaa acctggctga ttctgtgatg attgcttatg caccgatgga aatgcccatc
     3301 gtaatttctg atgactttat ggagtggatc tgatgtggcc gtttaaacga aaagaaccgt
     3361 cagcgccggc ggtaccggac aaacaacctg aaccacagcc accggaaatt aacgatgagg
     3421 tgatcgcttc cgtccggcag aaaccacgga gggagtttgt tcgctatgag ccaccaccgg
     3481 gcgttatccc ggcaccggtt cgcgatgctg tgctggcaat ggatgcaaca ccgtatgaca
     3541 ccctgaacag tcattatcct gattttgttt atggtggttt cccgggctat ccgtatctgg
     3601 cacttcaggc gcagttaccg gagtaccggc gtatggtcag tgtgcttgcc gaggagatga
     3661 cccgtaagtg gataaaggtt aaggcggttg gagaagggga cgacagccac gcgtcacgca
     3721 tagcgcagct tactgacgca ctggagcact ataacgtaca ggacgttttc agactggcga
     3781 ttgagcacga cggctttttc gggcgagggc agatttatat tgatgtgcgt tcgccatcgg
     3841 gtatatctgc ctggactgac ccggcggagc tgggatccgg actgtttatt tccgacaaaa
     3901 aaatcccgaa aggttccctg ctggggcttc gtgttattga acccgtctgg acgtatccgg
     3961 gtatatataa cgcggataat ccgctgagtg atgattttta ccgtccgtcc gaatggtacg
     4021 taatgggaaa aacggttcac gccagccgca tgattgatct gatctcccgt ccggttccgg
     4081 acatgctgaa gccggcctac aactttggcg gcctgttact ggttcagatt gctgagcctt
     4141 atgttgataa ctggctgcga acacgcgaca gcgtgggcga tatgctgcat tcgttttcgc
     4201 tgagcgggat taagacggat atgacacagg tattaagtgg taaaagggac ccgaattacg
     4261 caaaacgcgc ggagttgttt aaccgtaccc gtgataaccg cgggctgctg atgctggaca
     4321 agacgaacga agagtttttc cagttcaaca ctcccctgag cggcctcgac acccttcagg
     4381 cgcaggcaca ggaacacatg ttctttgtca gtgccatacc gtccgtaaaa ttcgccgggc
     4441 tgagcccaac gggactgaat gcgtcgagtg agggtgaaat ccgggtgttt tacgacacca
     4501 ttgctgcact tgccacccgt cttctgaaga aaccgctgaa aaaggtgctg gatattatcc
     4561 agttgtctga gttcggcgat atcgatcctg atatcacgtt tgaatttgaa cccctgcatg
     4621 agctgacgcg agagcaactg gcgaatatcc gtaaaactga ggcggaaacg gatcagattt
     4681 atgagagcac cggagcagtg accaataacg aagtacgcga acggctggcg actgcgcagg
     4741 acagcccgta cagcggtatt gacctgagcg gggacatcga aattgacgac actgaaattg
     4801 acaacaccga agaaaatccg ccgcaaggcc cgaatgcaga ccctgaggcg gatttcaccc
     4861 aacgcgggga ttgaggcctg gtaccgcaga caactggata atgccgtcag tgggatgcac
     4921 aacagtgtac tttactggct gcgggctgag taccgtaaaa cagacctcgc gcaggatgcg
     4981 tcccccgtta acctgatgcg tggtgccatg cagcaacttg cccggcgctg gcagaaaaat
     5041 tttgacgaaa tggccctgcg gctggcgagg cggtttgccg gtgatgtcct gaaaaacagc
     5101 gatgcgtcac tgtacactgc gctccgtgat gccgggttta cggttccttt ccgtatgaca
     5161 acggagatga ataccgcact tcaggccagc atcacggaga atgtgaacct cattcgctcc
     5221 atcccgcagc aacatctccc ccaggtggaa acactggtca tgcagtctgt tggccgggga
     5281 cgtgacctga aaacgctgac cgatgaactg gaaaaacgtt acggcatcac acgacggcgc
     5341 gcggcgctga ttgcccgcga ccagaacaat aaggcaacgt cggtaatgca ggccgccaga
     5401 cagcgttcgg tgggtatcac tgaaggtgtc tggcggcatt cccgcgcggg taaaacatgg
     5461 cgtccgtcgc atgtgaaggc gaacggtaaa cggtttgacc tgcgaaaggg gatgtttctg
     5521 gatggtaagt gggtactgcc gggcgaagaa atcaactgcc ggtgtggctg ggaggccgtt
     5581 attcccggac tggagaaaaa atgattatta ccgaaatgct ggcgtttgac cgggcatcgg
     5641 taaggcagtt cgataaagtc ggtcgcctcc agattgagcg cagtaatctc agcaaggcga
     5701 acgtctgcgg ttatttcggg tatgaaatac cgggggcgga agcgctggga ctcgacccca
     5761 aaaaacttta tcagctttac cgtgaccccg atgaactgcg caaggcagtt tcaaccttca
     5821 acaatattcc cgtcctgtgc cgacacaaac ccgattatcc gggcgcgccc gcgcgcgagt
     5881 accgggtggg gacgactcat gccaacagcg agtttgacgg tacctatctg gttaacggca
     5941 tgtccatctg ggacaactcc gccatcgcgg ggatagaaac ggatgaacaa cgggaaatct
     6001 catcgtcata tgcctatgtg gcagatatga cgccgggaac cacccccgac ggtgagctgt
     6061 atgacggcgt tatgcggaat atcgtgggaa atcatgtggc cctggtcggc gatggccggg
     6121 tagggtcgga ctgtcttgtt atggactctc tccctcagga gctaaaacgc atgaaactga
     6181 gtaaaaaaga agtggcggtg cttaccgcgc tgggaaccta tcttgcgccg cgtctggcgc
     6241 aggatgcggc tcccggggat ttgttacgcc tgatggcgca gtataagcgt ccggcagcta
     6301 tcgccagcgc ggtaaaaact gcttacagcg aacggctggt acaggatatg gatattgaac
     6361 cggtggagct ggcgcaactg atggaatcag cagaagccgt accggagctg gtcggggatg
     6421 atgataccgg gttaactgac gagtcgaagg catttgatac cgacagcccg atggaaagtg
     6481 tactggcgtt gctgtccggc aaagttcctg atgatgtgct ggaaaaaatt aaatccgcac
     6541 tgtctccgac aaccgacgaa gcccccgaaa caaaagaggc tgatgtgaaa cccgacgatg
     6601 tgaaagtcga taaacctgcg atggatgcag caatcaggct ggcaactgac caggcaacga
     6661 aacgggctgc tgaaaatttc cgcgccgtcc gtgtggctga agctgaagtg cgaccgctga
     6721 ttggcgatgt ggtggcgatg gactgcgccg aagatgttta ccgtaccgcg ctggaacaga
     6781 cggggatcga tattcagggt attcaccccg gtgcgtaccg cagcatggtg aagtttgccg
     6841 ttgagcagaa gcagacggcc aaatcctctc cccgtgtggc gatggatcag gccagcgcat
     6901 cgacgtttgc ggcagatttc cccggtgcaa aactgaaacg aggttactga tatgaatact
     6961 tttcagacac acatgaacca gtacccggca ccgggtattc cgggggcgtt tgccagtgct
     7021 aaccctcatg cctcgtatgt ggcgggagat ggcgggctga ttaccggccc tgacgggctg
     7081 gtaattgccc ggtttgcctg ggtaaccaaa ggtgttgccg ctaataaggg ggccggcgca
     7141 ccggcgggtt ttgttcctcg tgacgggcag gcttccattg tggaatggct ggccggggac
     7201 tcgaacacca tttatccggg gcgtgaatgc accctgatgg tgtcggggga tttctgggcg
     7261 ctgaccacca ctactgcgac gatcgtgcag aaagtatttg cctcactggc caccggggag
     7321 atagccacgg gggcggctgg cacctcgatg gcggggtttg tcgaaacctg gttttccgtt
     7381 gccagcgctg cggcggcaaa agaagtcatt aagatcagta cctggagcaa ataatgaata
     7441 aatttaaaca gcattatgcg acggcaagtc gcgactacgg gattattctt cccggagcac
     7501 aggcttattt gccgaaggag tacgctgccg attacggact ggcgatggac gcgcagcctg
     7561 cgctggtcac agcggctaac agcggtatcc ctgcgtattt caccaattac gttgagccag
     7621 aactgatccg cgtgctggtg acgccgatga aagccgctca gattctgggc gaaaccaaaa
     7681 aaggtgactg gacgacactg tcggcacaat tcccgattgc agaatctgac ggggaggtga
     7741 gttcctacgg ggattacagc aacaacgggg tggtaagcgc aaacgtcaac tgggtaccgc
     7801 gccagagcta tcacttccag accttcaccg tatggggcga acgtgagctg gagatgtacg
     7861 gcgcagcccg tattggctgg gcggcagagc tgaacgtggc gtcagcactg acgctgaata
     7921 agttccagaa taagtcctat ttctacggta ttgccggact gacgaactac ggtttgctga
     7981 atgatccgtc gttatctgca ccaatcaccc cggataccgt tgacggtaaa ctgaagtggg
     8041 acgacaagga cggacagggc gtgtatgacg atatcgtgaa actctttaag caactggtga
     8101 aacagactaa cggtcatatt gagcgtaccg acaaaatgaa actgtgcatg tcgccgctgg
     8161 cagaggtgaa cctcaccaaa acaaaccagt acagggttaa cgtgtccgat ctgctggcga
     8221 aaaatttccc ggcgatgacc attgaaacgg cggtggaata cacctctgac gctggcgagc
     8281 tggtacagct tatcgcggag cgtctgggtg aacaggatac aggctattgc gctttcactg
     8341 aaaaaatgcg cgcccatgcg gtagtggcag atacgtcttc ctggaaacag aaaaaatccg
     8401 ctggtacctg gggggcgatt attcgccagc cgctggcgta tgcacaaatg ctgggggtgt
     8461 gagttatggc tgaaatggta acagtgggct gcaaactgcc gaacggtctg gtgctggaag
     8521 tgggtcagaa acgggttcag gtggcaggct ggcggaataa cgcggttaaa attgtggggg
     8581 gctatggtct gacgcaggtc gataaggtgt tttttgacgc ctggctggcg gagcacggac
     8641 agcaacctta tgtgaaaaat ggcgttattt ttgcgcagga taaggcgaac agtgcagtct
     8701 cgcaggctac ggagcagaaa gccgttaaat ccggccttga accgctgccg cagaaaaatc
     8761 cggctccggg cgttaaccgc aatgatgaag tgatgggtaa acctcaggag taaacgctat
     8821 gggtacggtg acgtttgact gtcggatgtt tgtcagtctg tacccggagt tttccatggt
     8881 gacaccggag ctactgacgg cggtgtttaa ccaggcgacc gcgttgtacc tagataacac
     8941 ggacgacagt ccggttaccg acctgaagga gcgggaacag cttctgttcc tgctggttgc
     9001 gcatctgtgc gcattgcggg ggctgggaaa cgggagagac gggcaggctg gtctggtggg
     9061 gcgtatcacc agtgcgtcgg aaggttctgt atcggtatcg gtggataata gcggcagtaa
     9121 tgatgcgtcg tggtggtatc tccagacccc ctggggtgct gactactggc aggcgacagc
     9181 gccataccgc tcaatgcagt atcattccgg gggggcacct tcgcgatatc cggggcatta
     9241 ttaccgggga tacgggaggg gcgtcgatgg taaataaagt cacaggtggc aaacagttcc
     9301 ggcagaagct gaaacaggtt gccgcgaacc tttcattggg taaaaaactc aaagtgggtt
     9361 ttcttgaggg agcaacttac cctgacggta cgtcagtggc ttatattgcc gcagttcagg
     9421 agtttggtgg aagagcggtc atacccgccc gtgagcagac gctccacttt cgctataacg
     9481 aaaaaacggg agaaaccggg caccgctttg tcaaagccgg taaagggaat tttgttcagg
     9541 atgtggttat tcctgagcac acggtcacca ttcccccccg tccgttcttc cgtaagatga
     9601 tcgaacataa aagtcccgaa tggggcgaaa aaatggcgac cttattacgg gcgaatgatt
     9661 ttgatactgc gaccgctctg gtgtgtatgg gagaacatat caaagggcag ttgcagatgt
     9721 ctgttcgcga ctgggaaagt ccgcccaacg ctgcatccac tgcccggcaa aagggtttta
     9781 acaacccgct tattgaaacc ggacatatga tggacagcgt tgattattct gtagacgggg
     9841 gcaaaaaatg aactttcacg gtattgtttc cggcgcagta cgtcgggtaa atccctttac
     9901 agacgcgctg gtttatcgtt cccgcgggag tacacagcag gcagattatt cccgcgtacc
     9961 tgagtatgaa gatccggttt ccgtcagggt gcaaaagcag gctgtcaccc aggctgattt
    10021 acgtcatctt gataatctta accagcaggg agttttcgcc acattgtata ccgatggtaa
    10081 ctggtgcggg cttaaccgtg cctggcaaca gggaggcgat aaatttgtta ttggcgatga
    10141 aacgtggctg gtgattgagg taccggaaat ctggcctgac tggacgaggg ttattgtatg
    10201 ccttcaggtg tgaccctctc cgttacggaa agcgctcttt atcaggccct cggtgattat
    10261 ctccgggggc ttttttctga tgccgggatt gaacgaacgc agcagaacca ggtcccgatg
    10321 cctcagggga atttcatcac catgacgggc attgatgtta ccggattatc cactgcggta
    10381 gtggcatatt ctgcgccagc gcaggctggt gagggctctc agcatatcac ccgtaccaca
    10441 aaatggcgtt gccagcttga tttctacggc cctcacgcgg cagataacgc gcaggtgctg
    10501 gcaacgcttt tccggtctga attttccgta cggcttttcc ggcagggcgg tgggctgatt
    10561 tccccgctgt attgctcaga gccctttaac accacattcc ccaacggtca gcaacagtat
    10621 gaaccgcgct ggacgcttga tattcagatg cagattaacc ctgtggtcac aacttccctg
    10681 atgttttttg acaacgtgat cacccggaca acggaggctg ataatgccaa ttccactcag
    10741 taaagatgta cagataaatc ccggtgtgct ggctgtggcg ggtaatgccg ttgatcttaa
    10801 cggcctgctg ttgaccagta atccgttact tccggtcggc actgtggttc cgttttcctc
    10861 tccggatgat gtggccgcgt attttggtgc attatccgat gagtacgcac gcgctcagct
    10921 ttatttccag ggcttcaaaa atgccaccag gacgccgggg caattgttgt tttcccgttt
    10981 cagtcctgcc ccatcggcgg cctggttacg tagcggttcg tttaagggcg cgactgttga
    11041 acagctacaa aaactttccg gcacgctgat gctgagtatt aacgggaaaa gcgtcagcat
    11101 tgaagtgaat tttaacggtg tcgccagctt cgccgctgct gcaacggcac tacagacagc
    11161 gctgaccgca gcagtggcta cagtggtatt cgataccaca cagaatgctt ttgtcattac
    11221 tgtcgccggg gcgaaacccg ggagtaccac gataacgttc ggcagtggat cggcagcaga
    11281 actcctgaag atgaccagta acaccggtgc ggtgatatcc cggggcgcgc ctgtatctga
    11341 tgtccctgac acaatggcag ccattaaggc cgcttcccag caatgggcgg gtttttccac
    11401 ggtatctgag gtcactgacg agcaacacct ggcgttttct gcatgggcaa acgggcaggg
    11461 caagcgttac ttttatgtgg catggacaac cagcggcaac gccagagtaa aaggcagtac
    11521 tgaacacatc gcttaccaga taattgctgt caataactac agctctgttg tacctgtttt
    11581 cgcgactgat ggtaaccggg ctgcagcggt actggggtat gcagcgtgcc ttgattttgt
    11641 ccgaccagag gggcgtgtat cattcaagtt ccgcgagtat gaggggctgg ccgctgatgt
    11701 taccagtggc agcgattatg atgcgctgat cgccgcaggt tacaatttct acggaaaata
    11761 tgcggaaaac agtgtggtgg aagattactg ggcggatggc actattaccg gtgattttaa
    11821 atggctggac agtttctgcg ggcaaatctg gctgaatgcc agtttgcagg gggcggtgat
    11881 cgcgttattc aagtcaaacc agacgatccc ctacaacaat gaagggcggg cgctggttgc
    11941 agcgtcaatg agtgacgtta tccagcagta caaacgctgg ggagggatcc gtgaaggggt
    12001 gacactgacg gaggcgcaga agaaacagat taacaatgtt gtgggggcag atgtttcttc
    12061 aacgttgttt gccaccggct attacctgta tatcggcgat atgcttccct ctttgcgggc
    12121 accccgtagc agtccgtcct gtacgctctg gtactgtgac ggcggtagta tccagaaact
    12181 tgttattgca tccacggagg tccagtaaat gtcgggtaac aacaacacca tcactgcggc
    12241 ggatgccatt atcacgctga cagtgaataa tctgtatccc tccggcgtac gacttcaggg
    12301 atttgcaaca gataacgttt atggcaccga tccactggta ctggcggaaa ctgcccgcgg
    12361 tattgacggt aaactgtccg cgggatttgt gtacagcaac attatccaga cgtttcatat
    12421 catgccggat tcccccagcc gggatatttt tgatacctgg tcaaccacat cccgggccag
    12481 tcgggctgta ttccgttgta acgctgtcgt cctgcttccg gcgattggcc gtaaatatac
    12541 ctgcgtgaat ggcgtactca aacaatggaa agcgctgcct gatgcagctc gtacgttgca
    12601 gccagggcag gcggttattg agtgggaaac cattactccg gaggttttta actgatggcc
    12661 cgtaaagaga aatttatcac tattgaaggt cagggacggg ataagggcaa ggtatttcat
    12721 cttaccgaaa tgtctgcctc acaggcggaa tggtgggcga tgcgtgccat tatggcaatg
    12781 gggcgtggtg gcgtggagtt accggatgat gttcgtagtc tggggatggc tgcgcttgcc
    12841 cttgaagggc tgaaagcatt atcgaaaatt ccgcctgatg aggcgcgtcc gttgatggat
    12901 gaaatgatgg actgtgtgca gtttgttccc gatccgaaaa aacgcagcat ccggagaccg
    12961 cttgttgaag aggatattga agaaatcatt acccgtttta atctgcgtgc ggaggtcttt
    13021 aagctgcatg tggatttttt cgctaccgtc gtcagctaga tatccccccg tgttatctcg
    13081 accccgacag accgttcggg gtggtggatt acgttaatgt tccccgtacc attgcgaccg
    13141 ttatctcctc cggtaaggct tcaaaagctg aactggattc tgtccttggt gtgcaggact
    13201 tatgggatct gctggaaata attcatgtgg atgcccataa cgaacaggtg atacaggaga
    13261 acagaaatgg cgcaggtact tgatgagctt gttctgtccc tcgggattga tgacagggac
    13321 tttactgccg gggaacaggc agtaca
//
LOCUS       DADKXM010000110        13238 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.111, whole genome shotgun sequence.
ACCESSION   DADKXM010000110 DADKXM010000000
VERSION     DADKXM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.111"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..326)
                     /locus_tag="JQR05_003029"
     CDS             complement(<1..326)
                     /locus_tag="JQR05_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325132.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLM"
     gene            complement(643..1467)
                     /gene="iclR"
                     /locus_tag="JQR05_003030"
     CDS             complement(643..1467)
                     /gene="iclR"
                     /locus_tag="JQR05_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY5325133.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1667..5350
                     /gene="metH"
                     /locus_tag="JQR05_003031"
     CDS             1667..5350
                     /gene="metH"
                     /locus_tag="JQR05_003031"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY5325134.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQVESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYARDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTQPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETCTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5570..7201
                     /locus_tag="JQR05_003032"
     CDS             5570..7201
                     /locus_tag="JQR05_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY5325135.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7292..7969)
                     /gene="pepE"
                     /locus_tag="JQR05_003033"
     CDS             complement(7292..7969)
                     /gene="pepE"
                     /locus_tag="JQR05_003033"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY5325136.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFGVTQTWDD
                     YTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGLLAPI
                     TDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTNALPE
                     GHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGEEAVP
                     LEAGHRF"
     gene            complement(8351..9580)
                     /locus_tag="JQR05_003034"
     CDS             complement(8351..9580)
                     /locus_tag="JQR05_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5325137.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            complement(9632..10420)
                     /locus_tag="JQR05_003035"
     CDS             complement(9632..10420)
                     /locus_tag="JQR05_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY5325138.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10431..11228)
                     /locus_tag="JQR05_003036"
     CDS             complement(10431..11228)
                     /locus_tag="JQR05_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY5325139.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(11294..11788)
                     /locus_tag="JQR05_003037"
     CDS             complement(11294..11788)
                     /locus_tag="JQR05_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY5325140.1"
                     /translation="MNITLACIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11788..12195)
                     /locus_tag="JQR05_003038"
     CDS             complement(11788..12195)
                     /locus_tag="JQR05_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY5325141.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNP
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12205..13011)
                     /locus_tag="JQR05_003039"
     CDS             complement(12205..13011)
                     /locus_tag="JQR05_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5325142.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13081..>13238)
                     /locus_tag="JQR05_003040"
     CDS             complement(13081..>13238)
                     /locus_tag="JQR05_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY5325143.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
BASE COUNT         3164 a         3421 c         3482 g         3171 t
ORIGIN      
        1 tccattaggt attcttcaaa tattgtagca atggtttgag tgagtgtcgt gttttcgttt
       61 ttattaatta tcgtaatttc tttcttatca gcttgtaaga cggacgtgga aaatttaatg
      121 acaaaatcct tgttgctaaa gatatgacga acattttgtc tacagttctc tgagaagctt
      181 tttaatagag gcgtcgccag ctccttgcca gaaaatttat cctcgagttc tttataaaac
      241 aattcactca gggtttggtg ttcatttgtc tgggctgtat tattaatatt tgcagagaaa
      301 gaactacgag gaatacgagt aatcatttaa atatctcatt gtttattgat gtcttgaatt
      361 ttaactatca gaacagtgaa aaaatttaat atgattacaa ctaaaaaaat atcataaatc
      421 gctcaatctc ataatgcagc cgtaaaagtt tcggtggaat gagatcttgc gattttgtta
      481 ataattaagt ggtttgatgt ttaaaaataa tgctgagtta ataaaagtgt gtgaagtgta
      541 tgacattaag ttattttggc gtcaatgcga ttaacagaca cccttattct attgccactc
      601 aggtatgatg ggcagaatat tgcctctgcc cgccagaaaa agtcagcgca ttccaccgta
      661 cgccagcgtc acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcgatc
      721 atcggtaata cgtgaaatcg gtccggaaat agaaattgcg gcaaacggtt cgcggtgctc
      781 atcgaaaata cacgctgcaa ggcaacgtag ccccagcgca tgttcctcat cgtcaaatga
      841 ataaccccgt ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagagt
      901 tgcgtgggta taggcatgta accctttgcg gtgcagcagc ttcgtcacct gttcttcgct
      961 cagttgggct aaaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat
     1021 aggcgcggac attcgcatca ggtgggtaca ctgtacctgg tcgataataa tcgcttcgtg
     1081 atcgctttga tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg
     1141 caggataggg tgaacaattg ctaacaaatt acggctctgg agaaagctgc tgccgaccat
     1201 aaaggcatgt gcgccgattg cccagtgtcc cagttcgccg acctgacgca cgaaaccctg
     1261 ctgttgcatc gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgttgcgc
     1321 cagctctgtg agtgccacgc tgccattgga ttcggcaatc cactccagta atttcaggcc
     1381 acgcgttaaa gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc
     1441 gcgtttcgcg ggaatgggtg cgaccatgac agcctccttt ttctgtatcg tggaaattat
     1501 tttcattttt attgttagct aatgcaatag ttgctgaact gatccgatga gttaatgttg
     1561 aacaaatctc atgttgcgtg gtggtcgctt ttaccacaga tgcgtttatg ccagtatggt
     1621 ttgttgaatt tttattaaat ctgggttgag cgtgtcggga gcaagtgtga gcagcaaagt
     1681 ggaacaactg cgtgcgcagt taaatgaacg tattctggtg ttggacggcg gtatgggcac
     1741 catgatccag agctatcgac tgaacgaagc cgattttcgt ggtgaacgct ttgccgactg
     1801 gccatgcgac ctcaaaggca acaacgacct gctggtactc agtaaaccgg aagtgatcgc
     1861 cgctatccac aacgcctact ttgaagcggg cgcggatatc atcgaaacca acaccttcaa
     1921 ctccacgacc attgcgatgg cggattacca ggtggaatcc ctgtcggcgg aaatcaactt
     1981 tgcggcggcg aaactggcgc gcgcctgtgc tgacgagtgg accgcgcgca cgccggagaa
     2041 accgcgctac gttgccggtg ttctcggccc aaccaaccgc acggcgtcta tttctccgga
     2101 cgtcaacgat ccggcattcc gcaacatcac ttttgaccag ctggtggcgg cctatcgcga
     2161 atccaccaaa gcgctggtgg aaggtggtgc ggatctgatc ctgattgaaa ccgttttcga
     2221 cacccttaac gccaaagcgg cggtctttgc ggtgaaaacg gagtttgaag cgctgggcgt
     2281 tgagctgccg attatgatct ccggcaccat caccgacgcc tccgggcgca cgctctccgg
     2341 gcagaccacc gaagcatttt acaactcatt gcgccacgcc gaagcgctga cctttggcct
     2401 gaactgtgcg ctggggcccg atgaactgcg ccagtacgtg caggaactat cacggattgc
     2461 ggaatgctac gtcaccgccc acccgaacgc cgggctgcct aacgcctttg gtgaatacga
     2521 tctcgacgcc gacacgatgg caaaacagat acgtgaatgg gcgcaagcgg gctttctcaa
     2581 tatcgtcggt ggttgctgcg ggaccacgcc gcaacatatt gcagcgatga gtcgtgcagt
     2641 agaaggatta gcgccgcgca aactgccgga aattcccgta gcctgccgtt tgtctggcct
     2701 ggagccgctg aacattggcg acgatagcct gtttgtgaac gtgggtgaac gcaccaacgt
     2761 caccggctct gctaagttca agcgcctgat caaagaagag aaatacagcg aggcgctgga
     2821 tgtggcgcgt cagcaggtgg aaaacggcgc gcagattatc gatatcaaca tggatgaagg
     2881 gatgctcgat gccgaagcgg cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga
     2941 tatcgctcgc gtgccgatta tgatcgactc ctcaaaatgg gatgtcattg aaaaaggtct
     3001 gaagtgtatc cagggcaaag gcattgttaa ctctatctcg atgaaagagg gcgtcgacgc
     3061 ctttatccat cacgcgaaat tgttgcgtcg ctacggtgcg gcagtggtgg tgatggcctt
     3121 tgacgaacag ggacaggccg atactcgcgc acggaaaatc gagatttgcc gtcgggcgta
     3181 caaaatcctc accgaagagg ttggcttccc gccagaagat atcatcttcg acccgaacat
     3241 cttcgcggtc gccaccggta ttgaagagca caacaactac gcgcgggact ttatcggcgc
     3301 gtgtgaagac atcaaacgcg aactgccgca cgcgctgatt tccggcggcg tatctaacgt
     3361 ttctttctca ttccgtggca acgatccggt gcgcgaagcc attcacgcag tgttcctcta
     3421 ctacgctatt cgcaatggca tggatatggg gatcgtcaac gccgggcaac tggcgattta
     3481 cgacgaccta cccgctgaac tgcgcgacgc ggtggaagat gtgattctta atcgtcgtga
     3541 cgatggcact gagcgtttac tggagcttgc cgagaaatat cgcggcagta aagcggacga
     3601 cactgctaac gcccagcaag cggagtggcg ctcgtgggac gtgaataaac gtctggaata
     3661 ctcgctggtc aaaggcataa ccgagtttat cgagcaggat actgaagaag cccgccagca
     3721 ggctacgcgc ccgattgaag tgattgaagg cccgttgatg gacggcatga acgtggtcgg
     3781 cgatctgttt ggcgaaggca aaatgttcct gccacaggtg gtcaaatcgg cgcgcgtcat
     3841 gaaacaggca gtggcgtatc tcgaaccgtt tattgaagcc agcaaagagc agggtaaaac
     3901 caacggcaag atggtgatcg ccaccgtgaa aggcgatgtc cacgacatcg gtaaaaatat
     3961 cgttggtgtg gtgctgcaat gtaacaacta cgaaattgtc gatctcggcg ttatggtgcc
     4021 tgcggaaaaa attctccgta tcgccaaaga agtgaatgct gatttgattg gcctttcggg
     4081 gcttatcacg ccgtcgctgg acgagatggt taacgtggcg aaagagatgg agcgtcaggg
     4141 cttcaccatt ccgttactga ttggcggcgc gaccacctca aaagcgcaca cggcggtgaa
     4201 aatcgagcag aactacagcg gccctacggt gtatgtgcag aatgcctcgc gcaccgttgg
     4261 tgtggtagcg gcgctgcttt ccgatactca gcgtgatgat tttgtcgctc gtacccgcaa
     4321 ggagtacgaa accgtgcgta ttcagcacgg gcgcaagaaa ccacgcacac caccggtcac
     4381 gctggaagcg gcgcgcgata acgatttcgc atttgactgg caggcttaca cgcagccggt
     4441 ggcgcaccgt ctcggcgtgc aggaagtgga agccagcatc gaaacgctgc gtaattacat
     4501 cgactggaca ccgttcttta tgacctggtc gctggccggg aagtatccgc gcattctgga
     4561 agatgaagtg gtgggcgttg aggcgcagcg gctgtttaaa gacgccaacg acatgctgga
     4621 taaattaagc gccgagaaaa cgctgaatcc gcgtggcgtg gtgggcctgt tcccggcaaa
     4681 ccgtgtgggc gatgacattg aaatctaccg tgacgaaacg tgcacccatg tgatcaacgt
     4741 cagccaccat ctgcgtcaac agaccgaaaa aacgggattc gctaactact gtctcgctga
     4801 cttcgttgcg ccgaagcttt ctggtaaagc agattacatc ggcgcatttg ccgtgactgg
     4861 cgggctggaa gaggacgcac tggctgatgc ctttgaagcg cagcacgatg attacaacaa
     4921 aatcatggtg aaagcgcttg ccgaccgttt agccgaagcc tttgcggagt atctccatga
     4981 gcgtgtgcgt aaagtctact ggggctatgc gccgaacgag aacctcagca acgaagaact
     5041 gatccgcgaa aactaccagg gcatccgtcc ggcaccgggc tatccggcct gcccggaaca
     5101 tacggaaaaa gccaccatct gggagctgct ggaagtggaa aaacatactg gcatgaaact
     5161 cacagaatct ttcgctatgt ggcctggtgc atcggtttcg ggttggtatt tcagtcatcc
     5221 ggacagcaag tactacgcgg tggcgcaaat tcagcgcgat caggttgaag actatgcccg
     5281 ccgtaaaggt atgagcgtct ccgatgttga gcgctggctg gcaccgaatc tggggtatga
     5341 cgcggactga ttcacaaatc tgtcactttt ccttacaaca aacagggcgc tcaatgagtg
     5401 ccctgtctct ttattaatat gaaacactta tactggaaac aggctggaat aatcttagcc
     5461 ggaaaaacga atgtcaaaag gcacagtaaa gatgccagtc agcgtgtttc cggtcctttg
     5521 gtcattcaat tgcttcagga acatataacg ataaggagaa cctgaactcg tgttaacgct
     5581 gcttcacctg ctttctgccg tcgccctgct ggtctggggg actcatattg ttcgaaccgg
     5641 cgtaatgcgc gtcttcggcg cgcgtttgcg tactgtcctt agccggagcg tcgaaaaaaa
     5701 gccgctcgcc ttttgcgcgg ggatcggcgt taccgcactg gtacagagca gtaatgccac
     5761 caccatgctg gtgacctcgt tcgtcgctca ggatctggta gccctcgcac cggctctggt
     5821 cattgtgctg ggggcagatg tcgggacggc gctaatggcg cgtattctca ccttcgactt
     5881 atcctggctg tcaccgttac ttatttttat cggcgtgatt tttttcctcg gacgcaaaca
     5941 gtcacgcgcc gggcaactgg gccgcgtcgg tattggtctt gggctgattt tgctggcgct
     6001 ggagttgatt gtgcaggccg taacgccgat cacccaggca aacggcgttc aggtgatctt
     6061 tgcctcgctg accggcgata ttctgctgga tgcgctgatt ggcgcgatgt tcgccattat
     6121 cagctactcc agccttgctg ctgtactgct gaccgcgact ctgaccgctg caggcattat
     6181 ctccttcccc gtggcgctct gtctggtgat tggagctaac ctcggttccg gcctgctggc
     6241 gatgctcaac aacagtgccg ccaatgccgc agcccgccgt gtcgcgctgg gtagtctgct
     6301 gtttaagctg gtgggtagcc tgattatcct gccgtttgtc catttgctgg cagagacaat
     6361 ggggaagctg tcattgccaa aagcggaact ggtgatctat ttccacgtct tctacaacct
     6421 tgtacgttgc ctggtcatgc tgccatttgt tgacccgatg gcacggtttt gcaaaacgat
     6481 tattcgcgat gaaccggaac tggataccca gctacggcct aaacatctgg atgtcagcgc
     6541 gctggacacg cccacgcttg ctctggcgaa cgccgcgcgc gaaaccctgc gcattggcga
     6601 cgccatggaa cagatgatgg aagggctgaa taaagtgatg cacggcgagc cacggcagga
     6661 gaaagagctg cgtaagctgg cagatgatat caacgttctc tacaccgcca ttaagctgta
     6721 tctggcgcgg atgccaaaag aagagttggc ggaggaagag tcgcgccgct gggcggagat
     6781 catcgaaatg tcgctcaacc ttgaacaggc ctccgatatc gtcgagcgca tgggcagcga
     6841 aattgccgat aaatcgctgg cagcacggcg ggcattttcg cttgatgggt tgaaggaact
     6901 ggatgcgctc tatgagcaat tgctcagtaa tttaaagctg gcaatgtcgg tgttcttctc
     6961 tggcgatgtc accagcgctc gtcgtttgcg tcgcagcaaa catcgttttc gcattcttaa
     7021 tcgccgctat tcccacgccc acgtcgatcg cctgcatcag caaaacgtgc agagcattga
     7081 aaccagttcg ctacatttag gcttactggg agatatgcag cgcctgaact cgctgttttg
     7141 ttcggtggct tacagtgtgc tggaacagcc ggatgaagat gaaggacggg acgagtatta
     7201 atattgtgaa tcctgtaggc ctgataagac gcgtcaagcg tcgcatcagg cactatgaac
     7261 aagatgccgg atcatgcatc cggcaattca cttaaaaacg gtgaccagct tccagcggaa
     7321 ccgcttcttc accagcctta aacacataag tggtgttcgg gccacccagc gtagcgtgac
     7381 ctttactcac tgtgatccag ttaccttccg gcagaccaat aatcgtcagt tctggcgcga
     7441 cgaccagcag ttcgcgaata cgctgctcac gggtttcacc tttatggcct tccggcagcg
     7501 cgttggtgaa gtgcgggttg atttgcaacg ggaacagatt tagcgcatcg aaaccttgcg
     7561 gatcgacaat cggcatatcg ttggtggtac gaatggttgg gcaagcaagg ttagcgcctg
     7621 cgctccagcc aatatacagc gcgccacgtt taaccacgtc agtaattgga gccagcagcc
     7681 cgcgctcgcg gcactgtttc agcaactgga aagtattacc gccgccgaca atcacaattt
     7741 ccgcattttc aatcgcggca acgggatcga caacgctatg aataccggtg acagaaacac
     7801 ccagcggagc gagaaccgca gccgttttct ctgtgtaatc atcccaggtc tgcgttacgc
     7861 caaacaccgc tgagcggcga ccctgcaact gttcagcaat tagcggcagt gcatgttcca
     7921 gccaggcttt acccggcagc gtcgagttac tcaataaaag cagttccatt acttctccgg
     7981 ttagtaagaa atcacggaag gcatgctacc actgcgaatt taaccactta ctgacatggc
     8041 tcacgatgtt acgtattgca ccgggcgctg agactgatga caaacacagc attgcctgat
     8101 gcgctacgct tacctggcct accagataac tgcaatatat tgaattttca aaattatgta
     8161 gaccgaataa ggcttcattc cgcatccggc ataaacaatg tgtactttgc caccaatttg
     8221 agcgcccctt aaggcgctga tattttttat ccttctcgtg caaattgtgc tgcttcagca
     8281 atcagccgca catccggtcg cgaagttgct ggggcgacca gccagcgaca tgcagagtta
     8341 agtagcgcga ttaatcatgg gaaattgcct ctgcatgggc gagcaggtat tgctccgcct
     8401 caccggacca gatcccctga tgacgcgcca gaatcgccgc cagtgcttga tcctgaatct
     8461 gcgtgagtga cgtcagcggc aggtatttcc cggtatacac cagctttttg ccgccgccga
     8521 cggcaggcaa ttcaagcgtg gtttcgcccg cggcattcag cccaagaata tgtgttacca
     8581 cttttgcggc ctgcactttt ttctcttcga tcagtttgac cgccgcgcgc atgtcgtcgg
     8641 tattgccgcc tgacgtgccc acgtagtggg taaatgcata atgcacatcg tagaaattaa
     8701 ttggcgcgct gaaatgttta tcctgcggtc cggcgaagaa attcaggcaa ccatctgtcg
     8761 ccagcaagga agaggcgaga gtcaccagtc cttcattagg cacaaagacg aaaatatcat
     8821 cgaagccgtg accgccactc agcgccatta acgtatcaaa tgctgcatcg gtggcattga
     8881 gataatgaat cagtgtttgc ggttctgatg gatagtgctt gcgcgcataa ctcaatttgt
     8941 cgttgtcggt atcggtaatg acgagcagcg acgggttaac tggtccatgc agcgcataat
     9001 caatcgccaa cagtcccatt ggtccggtgc cgccgaggat cagcgtgcgc ccttgcgggc
     9061 gaatccccat cgtgtggtta taactacctt cctgaagatg atagttggcg ttgaacgcgc
     9121 caatcacgca ggaaagcggt tcaaccagtg agccttcaaa ataggtttcg ccgtcgtatg
     9181 ccagcaggca atcttgttcc atgacctcgt tgggaataac cacatgcgtc gcttcgccgc
     9241 ctacccacgg gaaggagtag ccggggcaat ccgggcggtc ggggagttgc aggttggcct
     9301 gaatcacata acgctgaccc ggctggaatt tgtgctgcca ctttttaccc acggccagaa
     9361 tatcgccgca aaattcgtgg ccgatgatga ttgggttggt cgccacatcg tcgggtactt
     9421 ttttatggtt ttcacccaga ttggcctctt tccaggaaga gaggcacagg ctgtcagtga
     9481 ccaccgttgc gaggatttca tcctcctgca tttcaggaag gtcaaaggtt tccaggcgta
     9541 aatcacgttt accataaaga cgcagagctg ttgttttcat aaaccacctc atgttgagcg
     9601 atggcctcgg cagtcgtgcc ggggcgaaga atcaggacag gaagcccaga gcattgccga
     9661 taatccctaa gccaaacagg gcgaaaatca gccataccgg gttaattttt ttgttgagca
     9721 gacgaaccat tagtagcgtc agaccgagcg ccagcaaacc agggcaaagt tggtcgagaa
     9781 tgttctgcac ggtcatggtg acggtggagc catcggcggc atgcgtttgt gaaaccacca
     9841 acggtacgtt gattgacgtc catttggtca ccagcacgcc catcacaaac agtccgagaa
     9901 ttgacgcgcc ttcggtgagt ttttgcagca cattcccgcc catatcgctg acgatattca
     9961 cccctttgcg aaagccgagc tgtaggccat accacttcat cgccagacgc accgcgttga
    10021 aaataaagaa gaacagcagc gggccgagaa tgttgcccga aagtgccaga gatgcgccga
    10081 gcgcggcggt aatcgggcgc agcgttcccc agaccagtgg atcgccaact cctgccaatg
    10141 gtcccatcag accgactttg atgccgttga tggtaccgtc atcaatttcc gcgccgatga
    10201 ccgggccaca tacggctggc gtggtattga agaacaccag gtgtcgcctg agcgccgcaa
    10261 cctgatcctc ttttaatggg taaagtcgct tgatggcggg gatcatgtcg tagcaaaagc
    10321 ccagcccgtg aatacgttca aagttaaagg acgcctgttg caggttggag cgcagaaaca
    10381 tgctcaccag atcgctgcgt gtaatttttc tctgttccat gatggctccg ttaatcgtca
    10441 agttggtcaa gggcggtgga ggcggtggcc tggagttgag gttcagcttt acgccactgt
    10501 ggatttagct ggatgtagac cagggccatg atcacgccga cgccaccgaa cgccagcagg
    10561 ctgagatcga ggtagccacc tgcgaggaaa ccgaggaaaa agaaaggcat caaatatttc
    10621 acgcccatca tacgtagcac catggcgtaa ccgaccacca cgataaaacc gccagcaatc
    10681 tgtagtcctc gggtcacaaa ttcgggaata gcgtttaaca tattgctgac catatcggcg
    10741 ctgacgaaca gtgagacaat cagtgccgga atggcgacgc gcagcgcctg cacgccaagc
    10801 gcggagacat gcagaatatc gagagtgcga aaccgcgcct cttctgctgc tttatccgcc
    10861 gcgtgctgga acactacggt gatggtacgg gcaaacaccg tcagcacttg gcctgccgca
    10921 gccacaggca acgcgatggc gattccggtg gcgatgcttt gctgaccaac gataaccaga
    10981 atggcggaga ttatgctggc gagtgcagaa tccggagatt gcgccgcgcc gacgttcatc
    11041 cagccgagag ctatcaattc cagcgtaccg ccgagtatta ttccggtttt taaatcaccg
    11101 agaattaaac caatcaccgt acaggcaatt aacgggcgat gagtctgaaa ttcatccagc
    11161 acgctgccca ttccggcaat acaggaaaaa agaaatatag caatgatttg tagggtactt
    11221 atttccatga tttgtcacct tttaagtgtc gtcatccctg cctgttaaag catatgtaca
    11281 caggcgctat tatttaattt gcgattaact gttcgtttat tttgtcgata atattgattg
    11341 agggatctga cgctacgacg cgtaaatcca gtatgacacc aagattattt aactcatgaa
    11401 atgcgttaat atcatcatca tctaatgaaa ccgctttggt taactgtttt ttaccgggcc
    11461 gccaggccat gccgccaata ttcagggtgc ctatttttac cccctggcga accatcgcta
    11521 aggcatcctg tgggcgagtg aaaagataaa aaacggtttc gtcctgatat tgcgggttgt
    11581 gataaaccgc gacggctttt tcaatattga ccacgttaac cttcattccc ggtggcgcag
    11641 cctgacgcaa taatgtccgg cgcacttcat cgttataaac ttcgtcattg cagataataa
    11701 tgcgctgggc gttagccact ttcgaccaga cggtagtgac ctggccgtgg atcagacggt
    11761 catcaatgca ggcgagcgta atgttcatca gaaatcctcc tctgtcgttg ccagttgttt
    11821 ccagacaacg caggtttgct ttgcgatttg cgtcaggtgt tcacacagtt catcaacatt
    11881 catgctgtcc gggttatcaa ccagttcgag cgccagcggc agagaaaggc cgctgattac
    11941 ccgcaaacga ggattgcgca tcgcaagcat tgctgccgca ttccacgggc tgccgcactg
    12001 taaatccacg gcaatcagcc actcatcttg attgtgaatg cttactaact tttccagctt
    12061 taccgcaatg tctccggcgt tttcaccggg cacaaattca acggcttcaa cattggcgtt
    12121 gccgtaaacc atctgaaccg attccagcat ggcgcaagct aatttgccgt gggcacaaaa
    12181 aatggcgttg accataaagc ctccttagcc gcgcgttttg ccgcccgcaa tgttagtggt
    12241 tactccggtg atgtagctgg cgcgttcaga caacagataa caaacaaaat cagctacttc
    12301 tgctaatctt ccggcgcgcc caataggaat ggcgtttttg gtatagcctt cacgcagctg
    12361 ctcgacggtg atattgcgcg tccacgccag cgcctcttca tattccggcg tgcgtagccc
    12421 ggttttttcc agaatcccgg gcgcgatacc gaccacacgg ataccgtgtt tacccagctc
    12481 tttcgaccag gagcgcgtga agctattgag cgcggctttg gtagcggcgt aacagctttg
    12541 tccttctgag ccttccagcc cactttctga ggaaacattc acaatcacgc catcatgttg
    12601 tttgaccatc tgtcgcgcca ccgcctgcga catcagaaaa acacctttct ggttgatatt
    12661 gaccattttt tcgaatgcag cttcgttgag ttcatactgc ccggcaggcg ctttctcatc
    12721 gaccagcaga cgcgggaaat tgaccccggc gttattgacc agaccgtcga tgcgaccaaa
    12781 acgctggata atttccgcta ccgtatgatt tacctctttg gtgctagaaa tatcggtcgg
    12841 ccaaaactga tagcctttat gaccttcata ttgaccatcg ccaccgtgaa tatcgaccat
    12901 ctgtacattc gcgccttgtg ctaataattc ctctacaatc gccagaccaa taccggatgc
    12961 gccgccggtg acaataatga ttttatcctg caaatttaac cacgtttgca taacagactc
    13021 ctgattttag ataataggga cccgccgtaa tttttattac ggtaaacatc gggaagattt
    13081 ttatttcagt aatagttcag ctgtgctgct attcgttacc agacaattaa tatattttcc
    13141 acgcagtgca ccaataattc cgctgtattt ttcttcactc atggcaatgc caatggaata
    13201 tcgcgcctgc tttaatttat tcatttcgat agagagcg
//
LOCUS       DADKXM010000111        13108 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.112, whole genome shotgun sequence.
ACCESSION   DADKXM010000111 DADKXM010000000
VERSION     DADKXM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.112"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JQR05_003041"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JQR05_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY5325144.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JQR05_003042"
     CDS             1828..3147
                     /locus_tag="JQR05_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY5325145.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3227..3649
                     /gene="tqsA"
                     /locus_tag="JQR05_003043"
                     /pseudo
     CDS             3227..3649
                     /gene="tqsA"
                     /locus_tag="JQR05_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3674..5062)
                     /gene="pntB"
                     /locus_tag="JQR05_003044"
     CDS             complement(3674..5062)
                     /gene="pntB"
                     /locus_tag="JQR05_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY5325146.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFGRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5073..6605)
                     /gene="pntA"
                     /locus_tag="JQR05_003045"
     CDS             complement(5073..6605)
                     /gene="pntA"
                     /locus_tag="JQR05_003045"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY5325147.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQSQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7129..8073
                     /locus_tag="JQR05_003046"
     CDS             7129..8073
                     /locus_tag="JQR05_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5325148.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8259..9641
                     /locus_tag="JQR05_003047"
     CDS             8259..9641
                     /locus_tag="JQR05_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5325149.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVSVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9678..10400
                     /gene="folM"
                     /locus_tag="JQR05_003048"
     CDS             9678..10400
                     /gene="folM"
                     /locus_tag="JQR05_003048"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY5325150.1"
                     /translation="MDKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10397..10732)
                     /locus_tag="JQR05_003049"
     CDS             complement(10397..10732)
                     /locus_tag="JQR05_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY5325151.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10861..11604
                     /gene="rstA"
                     /locus_tag="JQR05_003050"
     CDS             10861..11604
                     /gene="rstA"
                     /locus_tag="JQR05_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY5325152.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            11829..>13108
                     /locus_tag="JQR05_003051"
     CDS             11829..>13108
                     /locus_tag="JQR05_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY5325153.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGGGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSMMSGVISARLLK"
BASE COUNT         3217 a         3304 c         3154 g         3433 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgttg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc tgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cttggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccacca
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatca tcaccggcct
     3241 ggtcgcctgg gcgatgctcg ccgcactcga tgttcgcttc gcttttgtct ggggattgct
     3301 ggcctttgcg cttaattaca tccctaatat tggttcagtt ctcgcggcaa tcccccctat
     3361 cgctcaggta ctggtgttta atggcttcta cgaagcgttg ctggtgctgg cgggatatct
     3421 gctgattaat ctggtcttcg gcaatattct ggagccgcgc atcatgggac gtgggctggg
     3481 gctttcgaca ttggtggtat ttttgtcgtt gattttttgg ggatggttgt taggaccggt
     3541 gggtatgctg ctttccgtgc cgttgacaat tattgtcaaa attgcgcttg aacaaacagc
     3601 gggaggtcaa agcatcgccg ttctgttaag cgatctcaat aaagagtgac ggcctccgca
     3661 gaggccgtca gggttacaga gctttcagga ttgcatccac gctggctttg gcgtcaccaa
     3721 acagcatgtg ggtgttttcc ttgaagaaca gcgggttttg cacaccagca tagccagtgt
     3781 tcatcgaacg tttaaagaca atcacgttct gcgctttcca cacttccagc acaggcatac
     3841 cagcaatcgg actcttcgga tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa
     3901 tcaccagtac ggtatcggta tcagcaaagt catcgttgat ctcgtccatt tccagcacga
     3961 tgtcatacgg tacttttgct tcagccagca atacgttcat atgtccaggc aaacgccccg
     4021 caaccgggtg gataccgaaa cgcacattaa taccacgagc gcggagtttc tcggtaattt
     4081 cagcgacagg atattgcgcc tgcgcgactg ccatgccgta ccccggagta atgatcactg
     4141 aatgggagtt tttcagcagt tccgctgtct cttctgcggt gatttcgcgg tgctcaccca
     4201 cttcctgatc atcgccagta gaagagccgt cggtgccgaa accacccgca ataacgctga
     4261 taaaggaacg gttcatcgcc ttacacataa tgtaagaaag gatagccccc gaagaaccga
     4321 ccagcgcacc ggtcacaatc agcaggtcgt tgctgagcat aaagcccgca gccgcagccg
     4381 cccagccgga gtacgagttc agcatcgaca ccaccaccgg catatctgca ccaccgatgg
     4441 aggcgactaa atgccagccg aataccagcg caatggcggt cattatcagc aatgccagca
     4501 cttgcaggcc gacgctgtcc gtgcgaccaa atacaatcag cagcaagaag gaaacgacca
     4561 gagccgccag gttcattttg tgacggtttg gcagcatcaa cggtttagac gaaatcttgc
     4621 cacacagttt gccgaacgcc accaccgaac ccgtgaacgt taccgccccg atgaagatac
     4681 cgaggaacac ttccgtcagg tgaatattga ccagaatcgg tgccattccc gcgtcatgat
     4741 gcagataact gttaaagcca accagcactg ccgccagacc cacgaagctg tgcaggatcg
     4801 ccaccagttc tggcatttcg gtcatttcaa ctttcttcgc cagccggata ccaattgccc
     4861 caccaatgac catcgccagc aagatccagc caacattacc cgtatccggt ccaaaaatgg
     4921 ttgcgattaa cgcaatcgcc atcccggcga taccgaagtt gttaccctgg cgagacgttt
     4981 catgtttcga aagaccggcc agactgaaga taaacaggat cgcggcaaca atgtatgcag
     5041 ctgtaactaa tcctccagac atatgttacc ccttaatttt tgcggaacat tttcagcatg
     5101 cgctgagtca cggtgaagcc accgaaaata ttaatgctgg ctataagcac cgcgataaaa
     5161 ctaaggaagc taacccagcc gccctggcca atctgcaaca gtgctccgac aacaataatc
     5221 cctgaaatcg cgttggtaac cgacatcaac ggtgtatgca gcgcgtgcga tacattccac
     5281 accacgtaat aaccgacaac gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct
     5341 ttcggcgcaa cgcttgacag ccaaccgaac aggatgattg ccagcgccat caacgcgtat
     5401 ttacgccacg gtgagcaggc acatttttcc tcagttttca cttccggtgc cgctttttgt
     5461 gccgcctgcg actgagctga tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca
     5521 cggatcacgg tcacgccgcg aatcaccaca tcatcaaaat caacagtgat attgccgtct
     5581 ttctctttgc acaacagttt cagcagatta acgaggttag taccgtaaag ctgtgaggat
     5641 tgcgtcggca gacggcccgg aagatcggta taaccaatca ctttgacacc attttccgta
     5701 ctgaagattt cacccggcac ggtgtattca cagttgccac cgttttgggc tgccaggtcg
     5761 acaatcacac tgcccgcctt catggagtca accatttcac gggtaattag cttcggcgct
     5821 ggtttgcctg gaataagcgc ggtggtgaca atgatatcga cctcttttgc ctgggcggca
     5881 aagagttcca tttccgcttt gatgaacgcg tccgacatca ctttggcata gccatcgccg
     5941 ctgcccgctt cctctttaaa atccagctcg aggaattccg cgcccatact ttgaacttgt
     6001 tctttcactt ccgggcgggt gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg
     6061 ccaatggcgg ccagacctgc aacaccggca ccaatcacca tcacttttgc cggtggcact
     6121 ttcccggccg cagtgatttg cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca
     6181 atggcgcgat aaccggcgat attcgccatc gagcttagtg cgtccagcga ttgtgcgcgt
     6241 gagatacgcg gcacagagtc catcgccatc acggtcacgt tacgttccgc aagtttttgc
     6301 attaattccg gattctgcgc aggccagata aaactcacca gcgttgtccc gggattcagt
     6361 aacgcaattt catcatctaa cggcgcattg accttcagaa tgatctctga ctgccagacg
     6421 ctattccctt ctacaatttc agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc
     6481 agttgacccg cgccgctctc taccgcgacg gtaaaaccca gtttcagcag ctgttccact
     6541 gtttttggcg ttgctgcaac acgggtttca ttggttaacc gttctcttgg tatgccaatt
     6601 cgcatgatat tcccttccat cggttttatt gatgatggtt tgcctgtgtc aggagccaca
     6661 caagccgctc atgtacgagc taaatgttac tccgttaaaa taaattagta acaaacgccc
     6721 tataacgtac tgaaaattat gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa
     6781 ctcgcaaaat attaacgatt cagccgtgat agtgggataa acaccttaga acgccggata
     6841 aagactgata attgtcttcg acggtcgggt aaaacgagac atcgccccag cacgaatcac
     6901 tacttaacat taaattaact tatacaattc agttgcttca gtagtaatga tgctgatacg
     6961 gctgtttttt aagcatagac ggtcatttga gcaggattaa aattggttta aggaatgtga
     7021 tatgaaaaat gacgcagaca gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt
     7081 taataacaat accagtacct ggtttgcgca aggcgaagga ttatttttat gaagcttaag
     7141 aacaccctcc tggcgtcggc actgctttct gctacggcat tctccgttaa cgcagcaaca
     7201 gaactgacac cggagcaagc ggcagcggtt aaaccttttg accgtgtagt ggttaccggt
     7261 cgttttaatg ctattggcga agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt
     7321 gccgcctctt tttatgttgt cgacacttct gattttggta acagtggtaa ctggcgtgtg
     7381 gtcgctgacc tctataaagc cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt
     7441 aacggtgttg tcgaactgcc gaaagatcag gctgttctga ttgaaccgtt tgacacggtc
     7501 accgtccagg gcttctatcg tagccagcca gaagtcaatg atgccatcac caaagcggca
     7561 aaagcgaaag gtgcctactc tttctacatc gttcgtcaaa tcgatgccaa ccagggcggc
     7621 aaccagcgta ttactgcatt catctataaa aaagatgcta agaaacgtat cgtccagagc
     7681 ccggatgtga tcccggcaga ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa
     7741 gccgcgaaga aagttgagat cccgggtgtt gcgactaccg catcaccaag ttctgaagtc
     7801 ggtcgcttct ttgaaaccca gtcatcaaaa ggcgggcgtt acaccgtcac gctcccggat
     7861 ggcactaaag tcgaagaact gaacaaagcg acagcagcga tgatggtccc gttcgacagc
     7921 atcaaattct ctggcaacta cggcaacatg accgaagtct cctatcaggt tgcgaaacgt
     7981 gccgcgaaga aaggtgctaa gtattaccac atcacccgcc agtggcagaa acgtggtaat
     8041 aacctgaccg tcagcgcaga tctgtataaa taactattca gttactccta taaggcggct
     8101 tgatgccgcc tttcgcattt ttagtcacaa atttccccgc gaccattgca tgcagacaca
     8161 tctcttcgta aaatcccgcg cttcagtggc aaactccatt tttatgcgtt ttgccctaat
     8221 atttattctt cttatcacgt tttaatcact ggatatcgat ggaaaagaaa ctgggactga
     8281 gcgcactcac cgcgctggta ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc
     8341 aaaatatggc ggcagttgcc agcccggcag cactgctcat cggctggggt attactggcg
     8401 ctggcatttt attgctggcc tttgccatgc tgatcctcac gcgcattcgt cccgaacttg
     8461 atggcggtat ctttacctat gcccgcgaag ggtttggcga gctaatcggc ttttgttccg
     8521 catggggata ctggctgtgc gcggtcatcg ccaacgtctc ctatctggtg atcgtttttt
     8581 ccgcgttaag cttttttacg gacacgccgg aattgcgcct gtttggcgat ggtaatacct
     8641 ggcaatcgat agtcggtgca tcggctttat tgtggattgt tcactttttg atcctgcgcg
     8701 gggtgcaaac cgctgccagc attaacctgg tggcgacact ggcaaaattg ttgccgctgg
     8761 gtctgtttgt tgtgctggcg atgatgatgt tcaaactgga taccttcaag ctcgacttca
     8821 ccggacttgc acttggcgta tctgtttggg aacaggtgaa aaacaccatg ctgatcaccc
     8881 tgtgggtttt cattggtgtg gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac
     8941 gtgatgttgg caaagcgaca ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg
     9001 taacgctgct ttcgctgggt gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt
     9061 caatggccgg tctgatggtg gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg
     9121 gtttgatcgt ttccgtttgc ggcgcgtact taagctggac catcatggcg gcagaagttc
     9181 cgttcctggc tgccactcat aaagcattcc cacgcatttt tgcgcgccag aacgcacaag
     9241 ctgcgccatc cgcctcactg tggttgacca acatctgcgt gcaaatttgt ctggtactca
     9301 tctggctgac cggttccgat tacaacacgt tgctgaccat cgcctcagaa atgattctgg
     9361 tgccctattt cctggtcggc gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag
     9421 cggtaggtgt cggtgcctgc atttatggct tatggttatt gtacgcctct ggtccaatgc
     9481 acttgctgtt atccgttgtt ctgtatgcac cggggctgct ggttttcctg tatgcgcgca
     9541 aaacgcatac acatgataat gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt
     9601 taattgcctc agttccggcg acctggatgc tggtgggata acgttcatcc catcgttgga
     9661 taacaaagga gataacgatg gataaaaccc agcccttgcc aatattaatt actggcggag
     9721 gtcgtcgcat cggcctcgcc ctcgcatggc atttcattaa tcaaaagcaa ccggtgattg
     9781 tcagctatcg gacacactat ccagccattg atggactgat taatgcaggt gcgcagtgta
     9841 ttcaggctga tttttcgacc aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa
     9901 ccacccatgg tctgcgtgct attttgcata acgccagtgc gtggattgcg gaaaaaccgg
     9961 gtgcgccact ggccgacgta ctggcttgca tgatgcagat ccacgttaat accccatacc
    10021 tgctcaacca tgcgctggaa agattactgc gtgggcacgg acacgccgcc agcgatatca
    10081 ttcactttac cgattatgtg gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa
    10141 gcaaagcggc actggataat atgacccgct cgtttgcccg caagctggca ccggaagtga
    10201 aagtgaattc tattgcgcca tcgctgatcc tgtttaatga acatgatgat gccgaatatc
    10261 gacaacaggc gctgaataaa tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg
    10321 acctggtcga ttacttactt accagttgct ttgtcaccgg acgcagtttc ccacttgatg
    10381 gcggtcgtca tctgcgttaa tgcagcttta tccagcatat aatcaacacc catgcgctta
    10441 tcccccaaca cgcaacagaa ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat
    10501 accacagcga caccaggtaa acaaaatagg gaataatcga ccacatacta aaaatgatgg
    10561 ttgcgcgtaa ggcttcaatg ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa
    10621 aggtcgggaa aagtggaatc agcccggcga tataataatt tttcgttttt gctaaaacac
    10681 caatcaacag cactaccagc gcaccgagcg ctgctttgat taccagcccc atctttttac
    10741 cttaacactt ccataacaag tcatcagtag aatacctgat gaaaacttgt ttagaaacga
    10801 ttgatagtaa gtaaaaacag cgcgatgtat tgtgacgttt ttatatctac cgtgaatgtt
    10861 atgaacacta tcgtatttgt ggaagatgat gcggaagtcg gttcactgat tgccgcgtac
    10921 ctggcaaaac atgatatgca ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc
    10981 attttgcgag aaaatccgga tttggtgtta ctcgacatca tgctaccagg caaggacggc
    11041 atgaccattt gtcgtgattt acgcgcaaag tggtctggac cgattgttct tctaacctct
    11101 ctcgatagcg atatgaacca catcctggca ctggaaatgg gtgcctgcga ctatattctc
    11161 aaaacgacgc cccctgctgt tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag
    11221 caagccacac tgaccaaagg tcttcaggaa acgtctctga ctccctacaa agccctgcat
    11281 ttcggcacgt taactatcga ccccatcaac cgcgtagtca ccttggctaa cactgaaatc
    11341 tcgctctcga cagctgattt cgaattattg tgggaactag ctacgcatgc cgggcaaatc
    11401 atggaccgcg atgcattgct gaaaaattta cgcggcgtca gttatgacgg actggatcgt
    11461 agcgtggacg tggctatttc gcggttaaga aaaaaactgc tcgataacgc cgcagaacct
    11521 tatcgcatta aaactgtgcg taacaaaggc tatctttttg cgcctcatgc atgggatagt
    11581 gacaataaac aatcagatga ctaatatttg ttcatctgaa aaagagaaca attaacgaat
    11641 tcaattaata agagcttatt caaattccaa accccctaca tttgatatga atatgagaaa
    11701 aaatttctct ataatcgcct cgtccctgcg tcttatggcg cagatttttt acaacaaact
    11761 ctttccggca tttcaggtgc tgaatgaggc aagaggctta aaaaacggtt acaactttca
    11821 catatattgt gctttttttg atctgctcat gggggatttt caaaacggat caaaagagcg
    11881 aatccctttc caaaagtgtt tttatcgcga ttttcctggg ggctatcatt ggtatctccc
    11941 tgcatttcat tcctgagaca agcagtgaaa ccgttatcga atggtataat attgctggta
    12001 acggttatgt taatttatta aaacttgttg ccgttccact catattcatt tcgattcttt
    12061 cagcgattaa taagctcgaa aatagcgctg gtattggcaa gatgtcttta actattgttg
    12121 cctgtatgtt gtgtttagtc atgattgcag gtttagttgg tctggctatt gccagtgcaa
    12181 ttggccttga tgccagcgcc tttggtcata tgcaatcctc tttaaccagc gatgacgtct
    12241 cgaaaacagc tgcggtatct ttaccgcgac tcattacgtc attgatccca accaacctct
    12301 tccttgatct gaccggggca agaagtgttt cggttattgg catcgtcatc tttacaattc
    12361 ttgctggtat tgcgctgtta aaagtaaaga aagatgcacc agcagagggc gaaaaattga
    12421 gcgcggggat caacgcgatt caaatatggg taatgaaaat ggtccgtatt gtcattgcgc
    12481 tgacgcctta tggggtaatg gcactaatgg cgaaagtctt ctcaacgtat cagctttcac
    12541 agtttaccaa tttgctcggg ttcatcgtcg cctgttatct ggccgttttt gtgatgttta
    12601 ttgttcactc gggcattttg ctgttaatag gtaaaaaccc cgttaactat tacaagaccg
    12661 tctggcaagt acttaccttt gcgtttgttt ctcgcagtag tgcggcttca attcctctgg
    12721 ctatttctgc attggaaaaa ttgggggttc caaatacgat tgctaatatt gcagcgtctt
    12781 ttggttccag catgggacaa aatggctgcg ctgggattta tcctgccgtg atggtggcga
    12841 tgattgctcc aacaatgggt atcgatccct tctcattgca attcctcgca actcttttac
    12901 ctgccatcgc gcttggctca ataggggtag caggcgtagg tggtggtggg acattcgcgg
    12961 cgctgattgt cctttcaaca cttaatttcc ctgtcgcatt agtagttatt tttattgcca
    13021 tagagccgat cgttgatatg gcaagaacag cgcttaatgt gaatggttca atgatgtccg
    13081 gtgtaatatc agcccgtctt cttaagga
//
LOCUS       DADKXM010000112        12757 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.113, whole genome shotgun sequence.
ACCESSION   DADKXM010000112 DADKXM010000000
VERSION     DADKXM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.113"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..159
                     /locus_tag="JQR05_003052"
     CDS             <1..159
                     /locus_tag="JQR05_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY5325154.1"
                     /translation="ETYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKN
                     GEWNYIGK"
     gene            complement(300..1073)
                     /gene="uxuR"
                     /locus_tag="JQR05_003053"
     CDS             complement(300..1073)
                     /gene="uxuR"
                     /locus_tag="JQR05_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY5325155.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1288..2748)
                     /locus_tag="JQR05_003054"
     CDS             complement(1288..2748)
                     /locus_tag="JQR05_003054"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY5325156.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2829..4013)
                     /gene="uxuA"
                     /locus_tag="JQR05_003055"
     CDS             complement(2829..4013)
                     /gene="uxuA"
                     /locus_tag="JQR05_003055"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY5325157.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRLGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4353..5696
                     /gene="gntP"
                     /locus_tag="JQR05_003056"
     CDS             4353..5696
                     /gene="gntP"
                     /locus_tag="JQR05_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY5325158.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGMLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5947..6849)
                     /gene="fimH"
                     /locus_tag="JQR05_003057"
     CDS             complement(5947..6849)
                     /gene="fimH"
                     /locus_tag="JQR05_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY5325159.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6869..7372)
                     /gene="fimG"
                     /locus_tag="JQR05_003058"
     CDS             complement(6869..7372)
                     /gene="fimG"
                     /locus_tag="JQR05_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY5325160.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7385..7915)
                     /gene="fimF"
                     /locus_tag="JQR05_003059"
     CDS             complement(7385..7915)
                     /gene="fimF"
                     /locus_tag="JQR05_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY5325161.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7925..10561)
                     /gene="fimD"
                     /locus_tag="JQR05_003060"
                     /pseudo
     CDS             complement(7925..10561)
                     /gene="fimD"
                     /locus_tag="JQR05_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(10628..11353)
                     /gene="fimC"
                     /locus_tag="JQR05_003061"
     CDS             complement(10628..11353)
                     /gene="fimC"
                     /locus_tag="JQR05_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY5325162.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11390..11929)
                     /locus_tag="JQR05_003062"
     CDS             complement(11390..11929)
                     /locus_tag="JQR05_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5325163.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11994..12542)
                     /locus_tag="JQR05_003063"
     CDS             complement(11994..12542)
                     /locus_tag="JQR05_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5325164.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3046 a         3338 c         3105 g         3268 t
ORIGIN      
        1 gaaacctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
       61 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
      121 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
      181 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
      241 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
      301 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
      361 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
      421 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
      481 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
      541 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
      601 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
      661 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
      721 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
      781 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
      841 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
      901 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
      961 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     1021 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     1081 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     1141 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     1201 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     1261 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     1321 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     1381 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     1441 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     1501 ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg
     1561 ccagctgccg ccgttttgca agtgcagacg caccgggtcc agtagacgct gcggtaactt
     1621 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     1681 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     1741 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     1801 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     1861 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc
     1921 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     1981 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     2041 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     2101 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     2161 cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     2221 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     2281 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     2341 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     2401 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     2461 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     2521 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     2581 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     2641 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     2701 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     2761 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     2821 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     2881 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     2941 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     3001 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     3061 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     3121 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     3181 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     3241 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     3301 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     3361 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     3421 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     3481 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     3541 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctagacgttt caggatatgc
     3601 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     3661 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     3721 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     3781 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     3841 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     3901 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     3961 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     4021 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     4081 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     4141 ctaccttttg gtaagacatg tcaacctgaa ttctgaaatt ggttaaccaa atcacaagaa
     4201 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     4261 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     4321 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     4381 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     4441 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     4501 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     4561 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     4621 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     4681 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     4741 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     4801 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     4861 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     4921 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     4981 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     5041 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     5101 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     5161 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     5221 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     5281 gtgcgggtgg catgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     5341 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     5401 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     5461 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     5521 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     5581 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     5641 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     5701 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     5761 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     5821 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     5881 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     5941 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     6001 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     6061 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     6121 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     6181 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     6241 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     6301 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     6361 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     6421 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     6481 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     6541 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     6601 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     6661 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     6721 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     6781 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     6841 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     6901 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     6961 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     7021 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     7081 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     7141 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     7201 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     7261 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     7321 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     7381 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     7441 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     7501 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     7561 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     7621 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     7681 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     7741 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     7801 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     7861 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     7921 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     7981 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     8041 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     8101 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     8161 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     8221 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     8281 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     8341 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     8401 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     8461 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     8521 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     8581 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     8641 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     8701 agctggcact ggcatgtcac cactgagatt tactgtcaga acgcagccag tggctgaaag
     8761 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     8821 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     8881 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
     8941 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
     9001 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
     9061 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
     9121 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
     9181 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
     9241 tatccacaga tagagcgccc agtgccccca tgtttttccc gataccgaaa ttaaaagcac
     9301 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
     9361 gtaatgtact ctggaaaaat cggggttttt cctgctgcgc atttccacta cggtattctc
     9421 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
     9481 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
     9541 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
     9601 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
     9661 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
     9721 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
     9781 gcagggaacg taaaggaatt atgcctcgct caatccaggt aatgatatgc tgccatttat
     9841 ttttgccacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
     9901 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
     9961 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    10021 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    10081 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca
    10141 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    10201 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    10261 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    10321 cgacgcgata cgtccctggc ggtaattctt gcccatttta aaaacgcgat aaatcggcca
    10381 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    10441 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    10501 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    10561 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    10621 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    10681 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    10741 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    10801 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    10861 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    10921 tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt gacggaatcg
    10981 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    11041 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    11101 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    11161 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    11221 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    11281 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    11341 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    11401 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    11461 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc caatttgctg
    11521 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    11581 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    11641 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    11701 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    11761 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    11821 cgccctgaaa ttgcatattt ccgccgggaa gcgtggtatt ccatttattt ccggccagag
    11881 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    11941 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    12001 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    12061 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    12121 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    12181 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    12241 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    12301 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    12361 cgaacctgtc ccaactgaac agtttgatca acagagcctg catcaactgc gcaagcggcg
    12421 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    12481 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    12541 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    12601 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    12661 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    12721 ttggcttaat attctattgt tatctttatt tatagat
//
LOCUS       DADKXM010000113        12654 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.114, whole genome shotgun sequence.
ACCESSION   DADKXM010000113 DADKXM010000000
VERSION     DADKXM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.114"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            10..894
                     /locus_tag="JQR05_003064"
     CDS             10..894
                     /locus_tag="JQR05_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY5325165.1"
                     /translation="MLVTLATGRGLMLVGEPGTAKSLLSELLATSISGDAGLTIQGGA
                     STTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEVQDCLL
                     GMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFETVFPI
                     MDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAI
                     MSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQRVA
                     THKEAHWQAYYQARHRLP"
     gene            905..3184
                     /locus_tag="JQR05_003065"
     CDS             905..3184
                     /locus_tag="JQR05_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325166.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGNSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLAPFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3177..4313
                     /locus_tag="JQR05_003066"
     CDS             3177..4313
                     /locus_tag="JQR05_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY5325167.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSIVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4310..6302
                     /locus_tag="JQR05_003067"
                     /pseudo
     CDS             4310..6302
                     /locus_tag="JQR05_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
     gene            6427..6888
                     /locus_tag="JQR05_003068"
     CDS             6427..6888
                     /locus_tag="JQR05_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY5325168.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(6929..7399)
                     /locus_tag="JQR05_003069"
     CDS             complement(6929..7399)
                     /locus_tag="JQR05_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY5325169.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(7446..8165)
                     /gene="yehT"
                     /locus_tag="JQR05_003070"
     CDS             complement(7446..8165)
                     /gene="yehT"
                     /locus_tag="JQR05_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY5325170.1"
                     /translation="MIKVLIVDDEPLARENLCIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8162..9847)
                     /gene="btsS"
                     /locus_tag="JQR05_003071"
     CDS             complement(8162..9847)
                     /gene="btsS"
                     /locus_tag="JQR05_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY5325171.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10069..10134
                     /locus_tag="JQR05_003072"
                     /pseudo
     CDS             10069..10134
                     /locus_tag="JQR05_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            10362..10610
                     /locus_tag="JQR05_003073"
     CDS             10362..10610
                     /locus_tag="JQR05_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY5325172.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(10726..10995)
                     /locus_tag="JQR05_003074"
     CDS             complement(10726..10995)
                     /locus_tag="JQR05_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5325173.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            11052..11720
                     /locus_tag="JQR05_003075"
     CDS             11052..11720
                     /locus_tag="JQR05_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5325174.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            12035..>12654
                     /locus_tag="JQR05_003076"
     CDS             12035..>12654
                     /locus_tag="JQR05_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5325175.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEVIPDFPER
                     DDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTL"
BASE COUNT         3217 a         3227 c         3113 g         3097 t
ORIGIN      
        1 gtcgaacgta tgttagtcac tcttgccacc ggacgcggtt tgatgttggt gggggaacct
       61 ggcaccgcaa aatctctcct gtctgaatta ctggcaacct ctattagcgg cgatgccggg
      121 ttaaccatcc agggcggcgc atctactacc gaagatcaaa tcaagtacgg ctggaactac
      181 gccctgctta tcaatcatgg tccatcaacg gaagcgctgg tccctgcccc gctttaccag
      241 ggaatgcgcg acggcaaaat cgtccgtttt gaagagataa cgcgtacacc gctggaagtg
      301 caagactgtc tgttaggtat gctttctgac agggtgatga cggtgccgga actcactggt
      361 gaagccagtc agctctatgc gcgagaaggc tttaatatta ttgccactgc caatacccgc
      421 gatcgcggcg tcaatgaaat gagtgctgcg ctaaagcgtc gcttcgattt tgaaactgtc
      481 tttccgatta tggatttcgc ccaggagctg gagcttgtcg ccagtgcttc ggcgcgtttg
      541 ctggcccata gcggtattcc gcataaagtg cctgacgcgg tactggagtt actggtccgc
      601 accttccgcg atctgcgtgc caacggcgaa aagaaaacgt caatggatac actaacagcg
      661 attatgtcca ccgctgaagc cgtgaacgtg gcccacgccg taggcgtccg cgcctggttt
      721 ttagcgaatc gcgcaggtga gccagccgac ctggtggact gcattgccgg aaccatcgtc
      781 aaagataacg aagaagatcg cgctcgtctg cgccgttact tcgaacagcg tgtcgccaca
      841 cataaagaag ctcactggca ggcttattat caagcccgcc accgcctgcc gtgaggaaag
      901 atgcatgagc gagccgttaa ttgtcggcat ccggcatcat agtccggcct gcgcccggct
      961 ggtgaaatcg ttaatcgaaa gccagcggcc acgatacgtg ttgattgaag gcccggctga
     1021 ttttaatgac cgggtagacg aactgttttt agcccaccag cttccggtag ctatttacag
     1081 ttattgccag tatcaggacg gtgcagcccc cgggcgtggt gcctggacgc catttgctga
     1141 attttcgccg gagtggcagg cgctacaagc cgatcgtcgt attcaggcac aaacttactt
     1201 catcgatttg ccttgctggg cgcagagtga agaagaggac gattcgcctg atacgcaaga
     1261 ggaaagccag accttgctgc tgcgtgccac ccgcatggat aacagcgaca ccctgtggga
     1321 tcacttgttc gaagatgaaa gccagcaaac tgcattaccc tctgcactgg cgcactattt
     1381 tgcccaactg cggggcaact cccccggcga tgctctcaat cgtcagcgcg aagcctttat
     1441 ggcccgctgg attggatggg cgatgcagca aaataatggc gacgtgttag ttgtctgcgg
     1501 tggctggcac gctccggcac tggcaaaaat gtggcgcgaa tgtccgcagg taattaacac
     1561 gccagaattg ccgtcgctgg cagatgccgt tacaggttgt tatctcaccc cctacagtga
     1621 aaagcgcctt gatgtgctgg caggatacct ttcaggaatg cctgccccgg tctggcaaaa
     1681 ctggtgctgg cagtggggct tacagcaggc gggagaacaa ctgctaaaaa cggttctcac
     1741 ccgtttgcgc cagcacaagt tgcctgcttc gaccgcggat atggctgccg ctcatctgca
     1801 tgcgatggca ctggcacagt tgcgcggtca tacactaccg ttacgcactg actggctgga
     1861 tgccatagca ggatcgctga ttaaagaagc cctgaatgcg ccgttgccgt ggagctatcg
     1921 cggcgttatt catcccgata ccgatccgat tctgctaacg ttgatagaca cattagcggg
     1981 tgacggattc ggtaaacttg ccccttctac gccacaaccg cctctgccaa aagatgtcac
     2041 ttgcgaactg gaacgtaccg caatctctct tccggcggag cttaccttaa atcgctttaa
     2101 ccccaatgga ctagcgcaaa gtcaggtgtt acatcggctg gcaatactgg agatcccagg
     2161 gattgtacgc cagcagggaa gcacactgac acttgcaggc aacggtgaag aacgctggaa
     2221 attaacccgc ccgcttagcc agcatgcggc attgattgag gccgcctgct ttggtgccac
     2281 actccaggaa gccgcacgcc ataaattaga agccgatatg ctggacgcgg gtggaatcgg
     2341 cagcattacc acatgtctta gccaggcggc gtcagcgggt ctggcgccct tcagtcaaca
     2401 attactggag caactcacat tattaatcgc ccaggaaaat caatttgccg aaatgggcca
     2461 ggcgctggaa gtgctatatg ccttatggcg gctggatgaa attagcggta tgcaaggcgc
     2521 gcagatatta caaacgacgt tatgcgcggc tatcgatcgc acgctgtggc tgtgtgaatc
     2581 caacggcaga ccggatgaaa aggagtttca cgctcacttg catagctggc aagcgttgtg
     2641 tcatatcctg cgcgatctac atagcggtgt gcaactcccc ggcgtttcgc tttctgcggc
     2701 ggtagccttg ctggagcgac acagtcaggc gattcatgtc ctggcactgg atcgcggcgc
     2761 gactcttggt gcgctgatgc gtctggaaca tcccaacgct agtgccgaag cggcgctgac
     2821 gatgctggcg cagttatccc cggcacaatc cggcgaggcg ctgcacggtt tgctggcatt
     2881 agcccgtcat caactggcct gtcagccggc atttatcgcc ggtttcagca gtcatttaaa
     2941 tcaactaagt gatgccgatt ttaccaatgc cctgcccgat ttacgcgcgg caatggcctg
     3001 gctaccgcca cgagaacgcg ggacgctggc gcatcaggtg cttgagcatt atcaactggc
     3061 gcaacttccc gtttcggcac tgcaaatgcc gttgcattgt ccaccgcaag ccattgcaca
     3121 tcatcaacaa ctcgaacagc aggcactggc atcgctgcaa cactggggag ttttccatgt
     3181 ctgaactgaa cgatcttctg accacccgtg agctacaacg ctggcgatta attcttggcg
     3241 aagcggcaga aacgacgctt tgtgggctgg atgacaacgc ccggcagata gatcacgcgc
     3301 tggagtggct gtatgggcgc gatcctgaac ggctccagcg tggtgaacgc tctagtggat
     3361 taggtggctc aaatctcacc actcctgagt ggatcaacag tattcacacg ctgtttccgc
     3421 aacaggtgat tgagcggctg gaaagcgatg ccgtactgcg ctacggcatt gaagatgtgg
     3481 tgacaaatct cgacgtgctg gaacgtatgc agccttccga aagcctgcta cgcgccgttt
     3541 tgcacaccaa acatctgatg aaccccgaag tactggctgc cgcccgcagg attgtgcgcc
     3601 aggttgtgga agaaattatg gcgcgactgg caaaggaagt tcgtcaggct ttttctggtg
     3661 tccgcgatcg tcgtcgccgc tcatttattc cactggcgcg aaactttgat ttcaaaagta
     3721 ctctgcgcgc caacctgcaa cactggcacc cgcaacacgg caagttgtat atcgaattcc
     3781 cccgctttaa cagccgcatt aaacgccaaa gcgagcaatg gcaactggtc ttactggttg
     3841 atcaaagcgg atcgatggtc gattcggtga tccactctgc ggtgatggcg gcctgtttat
     3901 ggcagttacc cggcattcgt acccatctgg tggcgtttga cacaagcatc gttgatctca
     3961 cggcagacgt tgccgatccg gtagagttat taatgaaagt gcaattaggc ggcggaacca
     4021 atatcgccag tgccatggag tatggtcggc aacttattga acaacctgca aaaagcgtca
     4081 ttatcctcgt gagcgatttt tatgaagggg gttcatcatc attgctgacg caccaagtga
     4141 aaaagtgtgt ccagagcggc atcaaagtgc tgggactggc agcgctcgat agcacagcaa
     4201 caccttgcta tgaccgcgat acggcccagg cgctggttaa tgtcggcgca caaatagccg
     4261 ccatgacacc gggcgagctg gcatcatggc ttgcggagaa tcttcagtca tgaattcact
     4321 acgtccggaa ttattagaac tgacaccgca ggcactgacg gcgttaagca atgccggttt
     4381 tgttaagcgc agccttaagg aactggaaaa tggcaacgtc ccggaaatca gccatgagaa
     4441 cagcgcttta atcgccacct tcagtgacgg tgtccgtacc cagctggcga acggccaggc
     4501 actgaaagag gctcagtgca cctgtggggc cagcggcatg tgccgtcatc gcgtgatgct
     4561 ggtgttaagt tatcaacgac tttgtgctac cgcccagcct acgggaaaag aagaggaatg
     4621 ggatccggca atctggctgg aagaactggc tacccttccc gatgccaccc gcaaacgcgc
     4681 acaggcgctg gtcgctaaaa gcatcaccat tgagttgttc tgtgcgccgg gtgaaattcc
     4741 ctctgcccgc ttaccgatga gcgatgtgcg tttttattcc cgcagcagta ttcgtttcgc
     4801 ccgttgtgat tgtattgaag gcacactttg cgaacatgtc gtactggcag tacaggcctt
     4861 tgtccaggca aaagcgcagc aagcggaatt gactcattta atctggcaga tgcgcagcga
     4921 acacgtcaca tcatctgacg atccgtttgc cagcgaagaa ggcaaaacgt gtcgtcaata
     4981 tgttcaacaa ctaagccagg cattatggct gagcggcatc agccagccgc ttattcacta
     5041 cgaggccgct ttcagccgcg cgcagcaagc agcggaacgc tgcagctggc gatgggtgag
     5101 tgaatcgcta cggcaactgc gcgccagcgt tgatgccttc cacgcccgcg ccagccacta
     5161 tcatgccgga gaatgcttac gtcagcttgc ggcattaaac agtcgattaa attgcgcaca
     5221 agagatggcc cggagcgaca gtgttggtga agttcctcct gtgccgtggc gcacggtcat
     5281 tggctctggc attgccggag aagcaaagct tgatcatctg cggctggtgt ctttaggtat
     5341 gcgttgctgg caggatattg agcattatgg tttacgcatc tggtttaccg atcccgacac
     5401 cggcagtatt ttgcaccttt cgcgcagttg gccgcgaagt gaacaggaag actcaccggc
     5461 agctacgcgt cggctgttta gttttcaggc tggcgcactg gcgggtgggc aaattgtttc
     5521 acaagcagca aaacgcagtg ccgatggcga gctgctgtta gctacccgca accgcttaag
     5581 cagcgttgtg ccgctgtcgc ctgatgcctg gcaaatgttg agcgcgccgt tacgccagcc
     5641 gggcattgtg gctttgcggg aatatttacg ccagcgtccc cccgcctgca tacggcctct
     5701 taatcaggtc gataacttat ttattctgcc ggtcgctgag tgtatttcgc tcggttggga
     5761 tagcagccgc cagacgctgg atgcgcaggt aatcagcggc gaaggtgaag ataatctgct
     5821 gacgttatca ctgccagcct cagccagcgc gccttatgcc gttgaacgca tggcggcgct
     5881 tttgcaacaa acagacgacc ccgtgtgtct ggtttctggc tttgtcagtt ttgttgatgg
     5941 gcaattgaca ctggaaccac aggtaatgat aacaaagact cgtgcctggg cgctggacgc
     6001 agaaactgcg cctgtggtgg tatcactacc ttctgccagc gttctgcctg tgccgtctac
     6061 tgctcatcag ttgctgatgc gctgccaggc gttacttatt caactgctcc ataacggctg
     6121 gcgctatcag gaacagagcg ctattagtca ggcagagttg ctggcgaatg acctcaccgc
     6181 ggtgggtttt tatcggctgg cacatgtgtt ggcacaattt cgtaatacag aaagcgaggc
     6241 acgggtagaa gcaatgaata acggtgtttt gctttgcccc catgcttcag caacaaggat
     6301 gaaatagtgc tttttactaa gagttctact ccagttccgg actgctcacg ccacggtatt
     6361 aggcatatcc tatatagccc ctggtgagag tcaccagttt cttgattaaa taaaatggag
     6421 ttttacatga aggctttcaa taagctgttt tccctcgttg ttgcatctgt tctggttttc
     6481 tctcttgctg gctgcggtga caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc
     6541 actgaaattg ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa
     6601 accaaaattc aatttgcctc cattggtgca accaccaaag aagatgctgc caagacactt
     6661 gagccgttaa gcgccaaata caaaaacatc gcgggtgttg aagaaaaatc aacctatacc
     6721 gatacctacg cgcaggaaaa cgtgactatc gatatggaaa aagtggattt taaagccctg
     6781 cagggtattt caggaatcaa cgtttctgct gaagatgcca aaaaaggtat cactatggcg
     6841 caaatggaac tggtgatgaa agccgctggt tttaaagaag tgaaataatc tgtcggcggc
     6901 catgtttcgc atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca
     6961 gtccacgcag aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg
     7021 gtgcgcgcat catcgctgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga
     7081 tagccagaat gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc agcacgatgt
     7141 tgttattaat gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt
     7201 cataaatcag gccattgagg aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct
     7261 cttcgtcttc tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattatcca
     7321 gcgccagaat acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca
     7381 gaatatcgtt acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca
     7441 gtcttttaca ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg
     7501 gttaaaccat tacgcaaaat caactcggcc tggccgttat cttccaaacg aatctcctgt
     7561 aaatgcgcga ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc
     7621 agggtacgta atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca
     7681 ccgctcatcc gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta
     7741 tgccccgtac aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc
     7801 tgcttgctgc gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt
     7861 ggcttcagca gataatcaaa ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac
     7921 gcagtgagaa aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga
     7981 ccactgatgc gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc
     8041 gcgccgatcc cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc
     8101 tgcaaaaata tacacaggtt ctcccgtgct aacggttcat catcaacaat taagacttta
     8161 atcatgcctc gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac
     8221 aggcgacgct tattccatag tcatcgccaa accgttcacg taaacgctta tccaccagat
     8281 tcatccccag cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct
     8341 ccagcatcaa atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca
     8401 gttgtgatgt cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca
     8461 attgctgctg ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc
     8521 gcgccttttc aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa
     8581 actccgaagg ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc
     8641 tggcctgttc gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa
     8701 aatgaggatt cacctgggcg tgaagcagtt tgatttctga ctgggtgagc atcgcttttt
     8761 gccgctcata ttgcccggca agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca
     8821 gcgtgcggtt tattgaactg aataaacggt ttttggcttc atacaatttg atggtgccca
     8881 tcacacgctg attttcgcca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg
     8941 gatgcaaaga gcaacgataa ggtacttcgt tgccatcagc gtagaccact tcaccggttt
     9001 caatcgcttt taaggtgtaa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac
     9061 caattccggt aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca
     9121 gctcctgata cagcacctga gccactttca tgctgttcac ttcgttaaat ccctgtcgca
     9181 aaatgccttc cgtcgaggct gccactttca gcgcagtggc agaaaaagcc gaagtgtatt
     9241 tttcaaacat cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat
     9301 tggtgaccat cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc
     9361 gggcgatcgc aaggatgatc agcatttgca ccatttcagc gacgaacgtg acggcaccgg
     9421 cggtaatggg gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca
     9481 ggccgccgag caatccttca acgatggttg agatcatgca acttagcgcg gtcatgcccc
     9541 ccatcgaata tcgatgtaag ccgccggtca gaccaaccag cccaccgacg accggaccgc
     9601 cgagtaagcc gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt
     9661 gcaacccaaa ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt
     9721 tatgcggcag acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta
     9781 accacgcaat gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat
     9841 cgtacatacc cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta
     9901 actttctttg aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc
     9961 tggcaaaaat aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca
    10021 aaactgcgtc taaagttaaa ctgggacctc gcgagcaagg gtgagatgat ggcgctttac
    10081 acaattggtg aagtggcgtt gctttgtgat attaatcctg tcacgttacg cgcgtgacgc
    10141 aacattttta aaaatcgacg aagatcgtcc aggagcgccg tttttcaagg gttggataga
    10201 tgattcgttt accatgtcga cgaacttcga cgaatttcgc cgataaaaag tgtcccatac
    10261 atgccccatt gatgccccat aaacaatatc aggaattgac actggttatc catacagata
    10321 aaaataacac ctgtacataa ctacagttac aggagggtgt tatgcgtgtt gagatctgta
    10381 tcgctaaaga aaaaatcact aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat
    10441 taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa gccaccagca
    10501 acgatggcct ttctgttaca cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg
    10561 agactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa taaacacgta
    10621 aaatcggtaa cggctggaaa tcattcaata ctcgcactat cgaaagttta ccagccaacc
    10681 gcagcacgtc ctgcatacgt cgtgtctgcg gtttttcttt ttcgcttaca ttgtgtctgg
    10741 ttcttccggc cactcaatat caggtgcagt tgatgtatca acacggttca gcaacacccg
    10801 atacttcttc caggcttcca gcaacgagtt ttcttcctcc gttgcgatct ccagatctac
    10861 agcatcctga agtggcgcaa tatgctcact gaatccctgg atgtagaact gtgtggtgac
    10921 ggtcttccag ccattcggct cctgctgtat cgaagcatac caggctattt caatatcgct
    10981 atgctgcggc agcatttaac cccttgtaat tcatcgccat aattgattta attcacaaat
    11041 aaaactataa tatggtgaaa ttaatgaaaa aaaacacaga tgatggggct aaaatttaca
    11101 caccacttac cctaaagctt tatgactggt gggttttggg agtatcaaat cggcttgcat
    11161 ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta ggtaacaacc
    11221 atctggatat tggtgttgga actgggtttt accttactca cgtacctgag agtagtctga
    11281 tatctttaat ggatttgaac gaagctagcc tgaacgcggc atctacaagg gctggggaat
    11341 caaaaattaa acataaaatt agccatgatg tttttgaacc ttatcctgcg gcgttacatg
    11401 gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga aatatatcta
    11461 caaaaagctg tgtaatacgc aatgcggcgc aggccttaac tgatgatgga actctatacg
    11521 gagccacaat tcttggcgat ggagttgtgc acaatagctt cggtcaaaaa ctgatgcgca
    11581 tttacaatca gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc ttaacacata
    11641 tactctcaga gcatttcgag aatgttaaaa ccaaggttca aggtactgta gtaatgtttt
    11701 ccgcttcagg gaaaaaatag catccaaccg cagcacgttc ttgcttaaga cgtgctacgg
    11761 tttcctttac gatttggggt tgcgacttta cctctattga taatgcattc cggcagaacg
    11821 ttcaaatatg aacgttcgat atttaactaa ccgaaaaaca aaatatagca caggcgagac
    11881 atttaccatc taagaaaata gtatcgtttt ttacagttaa atcaacatca cttccttaaa
    11941 atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gtttattatt tcccagtatc
    12001 acattttatc agaatatcct tctgcgaggt tattatgctt cctgtaaata atccccccct
    12061 atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc acaataatta
    12121 tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc
    12181 ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact tatcttgttt
    12241 aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg taagcaataa
    12301 ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg caagcagtaa
    12361 tcaattatct gcacttcctg aattacctgc gtcactagaa tatattaatg taagtgacaa
    12421 tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg cagccagaaa
    12481 tgctttggaa gtaataccag attttccaga aagagatgat catattataa gaatattctg
    12541 gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca gttctgatag
    12601 cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DADKXM010000114        12239 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.115, whole genome shotgun sequence.
ACCESSION   DADKXM010000114 DADKXM010000000
VERSION     DADKXM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.115"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..203
                     /gene="xylF"
                     /locus_tag="JQR05_003077"
     CDS             <1..203
                     /gene="xylF"
                     /locus_tag="JQR05_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="periplasmic substrate-binding component of the
                     ATP-dependent xylose transport system; high affinity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose transporter subunit XylF"
                     /protein_id="HAY5325176.1"
                     /translation="KPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPI
                     DVNKNNIKDTVIKDGFHKESEL"
     gene            281..1822
                     /gene="xylG"
                     /locus_tag="JQR05_003078"
     CDS             281..1822
                     /gene="xylG"
                     /locus_tag="JQR05_003078"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY5325177.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            1800..2981
                     /gene="xylH"
                     /locus_tag="JQR05_003079"
     CDS             1800..2981
                     /gene="xylH"
                     /locus_tag="JQR05_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY5325178.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            3059..4237
                     /locus_tag="JQR05_003080"
                     /pseudo
     CDS             3059..4237
                     /locus_tag="JQR05_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(4345..5169)
                     /locus_tag="JQR05_003081"
     CDS             complement(4345..5169)
                     /locus_tag="JQR05_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY5325179.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            5489..7519
                     /gene="malS"
                     /locus_tag="JQR05_003082"
     CDS             5489..7519
                     /gene="malS"
                     /locus_tag="JQR05_003082"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY5325180.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7697..8950
                     /gene="avtA"
                     /locus_tag="JQR05_003083"
     CDS             7697..8950
                     /gene="avtA"
                     /locus_tag="JQR05_003083"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY5325181.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRANGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(9101..9574)
                     /locus_tag="JQR05_003084"
     CDS             complement(9101..9574)
                     /locus_tag="JQR05_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5325182.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(9676..10524)
                     /gene="yiaJ"
                     /locus_tag="JQR05_003085"
     CDS             complement(9676..10524)
                     /gene="yiaJ"
                     /locus_tag="JQR05_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY5325183.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            10725..11723
                     /gene="yiaK"
                     /locus_tag="JQR05_003086"
     CDS             10725..11723
                     /gene="yiaK"
                     /locus_tag="JQR05_003086"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY5325184.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            11735..12202
                     /locus_tag="JQR05_003087"
     CDS             11735..12202
                     /locus_tag="JQR05_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY5325185.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         3089 a         3065 c         3069 g         3016 t
ORIGIN      
        1 ataaacctat tacattgctg gcaaatactg ccgcagaaat tgccgttgaa ttgggcaatg
       61 gtcaggagcc aaaagcagat acctcactga ataatggcct gaaagatgtc ccctcccgcc
      121 tcctgacacc gatcgatgtg aataaaaaca acatcaaaga tacggtaatt aaagacggat
      181 tccacaaaga gagcgagctg taagcgttac gccccggcgc ggagcggggg cgtgatttct
      241 ctccatgccg cgtgaatgaa ttggcttagg tggagtcgtt atgccttatc tacttgaaat
      301 gaagaacatt accaaaacct tcggcagtgt gaaggcgatt gataacgtca gcttgcggtt
      361 gaatgctggc gaaatcgtct cactctgtgg ggaaaatggg tctggtaaat caacgctgat
      421 gaaagtgctg tgtggtattt atccccatgg ctcctacgaa ggcgaaatta tttttgcggg
      481 agaagagatt caggcgagtc acatccgcga taccgaacgc aaaggtatcg ccatcattca
      541 ccaggaattg gccctggtga aagaattgac cgtgctggaa aatatcttcc tgggtaacga
      601 aataacccac aatggcatta tggattatga cctgatgacg ctacgctatc agaagctgct
      661 cgcacaggtc agtttatcca tttcacctga tacccgcgtt ggcgatttag ggcttgggca
      721 acaacaactg gttgaaattg ccaaggcact taataaacag gtgcgcttgt taattctcga
      781 tgaaccgaca gcctcattaa ctgagcagga aacgtcggtt ttactggata ttattcgcga
      841 tctacaacag cacggtatcg cctgtattta tatttcgcac aaactcaacg aagtcaaagc
      901 gatttccgat acgatttgcg ttattcgcga cggacagcac attggtacgc gtgatgctgc
      961 cggaatgagt gaagacgata ttatcaccat gatggtcggg cgagagttaa ccgcgcttta
     1021 ccctaatgaa ccacatacca ccggagatga aatattacgt attgaacatc tgacggcatg
     1081 gcatccggtc aatcgtcata ttaaacgagt taatgatgtc tcgttttccc tgaaacgtgg
     1141 cgaaatactg ggtattgccg gactcgttgg tgccggacgt accgagacca ttcagtgcct
     1201 gtttggcgtg tggcccggac aatgggaagg aaaaatttat attgatggca aacaggtaga
     1261 tattcgtaac tgtcagcaag ccatcgccca ggggattgcg atggtacccg aagacagaaa
     1321 gcgcgacggc atcgttccgg taatggcggt tggtaaaaat attaccctcg ccgcactcaa
     1381 taaatttacc ggtggcatta gccagcttga tgacgcggca gagcaaaaat gtattctgga
     1441 atcaattcag caactcaaag ttaaaacgtc gtcccccgac cttgctattg gacgtttgag
     1501 cggcggcaat cagcaaaaag cgatcctcgc ccgctgtctg ttacttaacc cgcgcatcct
     1561 cattcttgat gaacctacca ggggtattga tattggcgcg aaatatgaga tctacaaatt
     1621 aattaaccaa ctcgtccagc agggtattgc cgttattgtc atctcttccg aattacctga
     1681 agtgctcggc cttagcgatc gtgtactggt gatgcatgaa gggaaactaa aagccaacct
     1741 gataaatcat aacctgactc aggagcaggt gatggaagcc gcattgagga gcgaacatca
     1801 tgtcgaaaag caatccgtct gaagtgaaat tggccgtacc gacatccggt ggcttttctg
     1861 ggctgaaatc actgaatttg caggtcttcg tgatgattgc agctatcatc gcaatcatgc
     1921 tgttctttac ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc tccaacctgt
     1981 tacgccagac cgcgattacc ggcatcctcg cggtaggaat ggtgttcgtc ataatttctg
     2041 ctgaaatcga cctttccgtc ggctcaatga tggggctatt aggtggcgtc gcggcgattt
     2101 gtgacgtctg gttaggctgg cctttgccac ttaccatcat tgtgacgttg gttctgggac
     2161 tgcttctcgg tgcctggaac ggatggtggg tcgcgtaccg caaagtccct tcatttattg
     2221 tcaccctcgc gggcatgttg gcatttcgcg gcatactcat tggcatcacc aacggcacga
     2281 ctgtatcccc caccagcgcc gcgatgtcac aaattgggca aagctatctc cccgccagta
     2341 ccggcttcat cattggcgcg cttggcttaa tggcttttgt tggttggcaa tggcgcggaa
     2401 gaatgcgccg tcaggctttg ggtttacagt ctccggcctc taccgcagta gtcggtcgcc
     2461 aggctttaac cgctatcatc gtattaggcg caatctggct gttgaatgat taccgtggcg
     2521 ttcccactcc tgttctgctg ctgacgttgc tgttactcgg cggaatgttt atggcaacgc
     2581 ggacggcatt tggacgacgc atttatgcca tcggcggcaa tctggaagca gcacgtctct
     2641 ccgggattaa cgttgaacgc accaaacttg ccgtgttcgc gattaacgga ttaatggtag
     2701 ccatcgccgg attaatcctt agttctcgac ttggcgctgg ttcaccttct gcgggaaata
     2761 tcgccgaact ggacgcaatt gcagcatgcg tgattggcgg caccagcctg gctggcggtg
     2821 tgggaagcgt tgccggagca gtaatggggg catttatcat ggcttcactg gataacggca
     2881 tgagtatgat ggatgtaccg accttctggc agtatatcgt taaaggtgcg attctgttgc
     2941 tggcagtatg gatggactcc gcaaccaaac gccgttcttg attttgataa aaattttctc
     3001 aaagccggtt acgtattacc ggttttgagt ttttgcatga ttcagcagga aaagaaccat
     3061 gtttactaaa cgtcaccgca tcacattact gttcaatgcc aataaagcct atgaccggca
     3121 ggtagtagaa ggcgtagggg aatatttaca ggcatcacaa tcagaatggg atattttcat
     3181 tgaagaagat ttccgcgccc gcattgataa aatcaaggac tggttaggag atgtcgtcat
     3241 tgccgacttc gacgacaaac agatcgagca agcgctggct gatgtcgacg tccccattgt
     3301 tggggttggc ggctcgtatc accttgcaga aagttaccca cccgttcatt acattgccac
     3361 cgataactat gcgctggttg aaagcgcatt tttgcattta aaagagaaag gcgttaaccg
     3421 ctttgctttt tatggtcttc cggaatcaag cggcaaacgt tgggccactg aacgcgaata
     3481 tgcatttcgt cagcttgtcg ccgaagaaaa gtatcgcgga gtggtttatc aggggttaga
     3541 aaccgcgcca gagaactggc aacacgcgca aaatcggctg gcagactggc tacaaacgct
     3601 gccaccgcaa accgggatta ttgccgttac tgacgcccga gcgcggcata ttctgcaagt
     3661 atgtgaacat ctacatattc ccgtaccgga aaaattatgc gtgattggca tcgataacga
     3721 agaactgacc cgctatctgt cgcgtgtcgc cctttcttag gtcgctcagg gcgcgcggca
     3781 aatgggctat caggcggcaa aactgttgca tcgattatta gataaagaag aaatgccgct
     3841 acagcgaatt ttggtcccac cagttcgcgt cattgaacgg cgctcaacag attatcgctc
     3901 gctgaccgat cccgccgtta ttcaggccat gcattacatt cgtaatcacg cctgtaaagg
     3961 gattaaagtg gatcaggtac tggatgcggt cgggatctcg cgctccaatc ttgagaagcg
     4021 ttttaaagaa gaggtgggtg aaaccatcca tgcaatgatt cataccgaga agctggagaa
     4081 agcgcgcagt ctgctgattt caaccacctt gtcgatcaat gagatatcgc aaatgtgcgg
     4141 ttatccatcg ctgcaatatt tctactctgt ttttaaaaaa gcatatgaca caacgccaaa
     4201 agagtatcgc gatgtaaata gcgaggtcat gttgtagcga tgatgagaat tgtcggcgtc
     4261 acatcaggga actcagcaca gccacctgat gcgagcaaaa gccttatccg accgacaaat
     4321 cagataaggc tcgaggaaat gaaattacat atgcgccgcg attaaccgtt ggttatcttg
     4381 gtacattgcg aacaggtagt tgttataact cttccctttg gtcgagtagc ccttcagctt
     4441 gtgaatcatc gctgtggcag tcacttcctg atccgcttta cgcagctgtg cacgcgattt
     4501 acggaacgaa gagtaagccg ggtgcgtatt caggttagtg acataggcgc tcaccgattc
     4561 tttgacagaa ctaaactgtg agtacccttt cactttaccc ggcgcattgg tacaacgtcc
     4621 tttcatgcat ttcatgccga acagattgtt attgttgcgc gccagcttcg acgttcccca
     4681 accgctttct gctgcagcca tcgtcgccac catactggta gggataatgt ctacgcgttc
     4741 aagcaaggta ttccacggga tttttcgcgt attaccagac cacttcacct tgtagcgttt
     4801 ggcgatgtct ttcagacgcg cacgctcagc aggtgaccat tggccctgat actgttttga
     4861 aatcagccag ttacgttctg cagtaatggc tgcattttgg ctggtaatgt aaggcattac
     4921 ggtccggaga aacgcctttt tccttggtgt tccggaaggg tattttcgca aatcaggaag
     4981 tgaactactc tttgcactat tgcgagaata ctcttgttta ctgcttacct gtttattact
     5041 agctttagtt aagtgggact tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc
     5101 actcgaaaac accagagtga gtaacataag aatcatcgcc ccatatcgtc gtatgggagt
     5161 caaaatcatc aggtctcctg gtcggattta atcattccaa caccttatat ttttcacaaa
     5221 tttgagagtt gaatctcaaa tcatatcaaa aatagctgtc aagagcaccc caaggaatag
     5281 tccaaatctg aaactatgtc acgagttaac gattcagatt ggcgctaaat cgcagaaaat
     5341 gtgggggtta tcgcaaaatt cagccgtttt ttgcgcgcga tcgctcaccc ttgcttctca
     5401 tcctgtggac ttaccgctca gggatgagtt ttgtctggct tatcgctggc aaactgtctg
     5461 aaatcgcagc aataaggact catccaccat gaaactcgcc gcctgttttc tgacactcct
     5521 tcctggcttc gccgttgccg ccagctggac ttctccgggg ttccctgcct ttagcgaaca
     5581 gggaacggga acatttgtca gccacgcgca gttgcccaaa ggtacgcgtc cactcacgct
     5641 aaattttgac caacagtgct ggcagcctgc agatgcgata aaactcaatc agatgctttc
     5701 cctgcaacct tgtagcaaca cgccgcctca atggcgattg ttcagggacg gcaaatatac
     5761 gctgcaaata gacacccgct ccggtacgcc aacattgatg atttccatcc agaacgccgc
     5821 cgaaccggta gcaaacctgg tccgtgaatg cccgaaatgg gatggattac cgctcacgct
     5881 ggatgtcagc gccactttcc cggaaggagc cgccgtacgg gattattaca gccagcaaat
     5941 tgcgatagtg aagaacggtc aaataacgtt acaacccgct gctaccagca acggtttact
     6001 cctgctggaa cgggcagaaa ctgacacatc cgcccctttc gactggcata acgccacggt
     6061 ttactttgtg ctgacagatc gtttcgaaaa cggcgatccc agtaatgacc agagttacgg
     6121 acgtcataaa gacggtatgg cggaaattgg cacttttcac ggcggcgatt tacgcggcct
     6181 gaccaacaaa ctggattacc tccagcagtt gggcgttaat gctttatgga taagcgcccc
     6241 atttgagcaa attcacggct gggtcggcgg cggtacaaaa ggcgatttcc cgcattatgc
     6301 ctaccacggt tattacacac aggactggac gaatcttgat gccaatatgg gcaacgaagc
     6361 cgatctacgg acgctggttg atagcgcaca tcagcgcggt attcgtattc tctttgatgt
     6421 cgtgatgaac cacaccagct atgccacgct ggcggatatg caggagtatc agtttggcgc
     6481 gttatatctt tctggtgacg aagtgaaaaa aacgctgggt gaacgctgga gcgactggaa
     6541 acctgccgcc gggcaaacct ggcatagctt taacgattac attaatttca gcgacaaaac
     6601 aggctgggat aaatggtggg gaaaaaactg gatcagaacg gatatcggcg attacgacaa
     6661 tcctggattc gacgatctca ctatgtcgct ggcctttttg ccggatatca aaaccgaatc
     6721 aactaccgct tctggtctgc cggtgttcta taaaaacaaa acggatactc acgctaaagt
     6781 catcgaaggc tttacacctc gcgattactt aacccactgg ttaagtcagt gggtccgcga
     6841 ctatgggatt gatggttttc gggtcgatac cgccaaacat gttgagttgc ccgcctggca
     6901 gcaactgaaa accgaagcca gctccgcgct tcgcgaatgg aaaaaaacta accccgacaa
     6961 agcattagat gacaaacctt tctggatgac cggtgaagcc tggggccacg gcgtgatgca
     7021 aagtgactac tatcgccacg gcttcgatgc gatgatcaat ttcgattatc aggagcaggc
     7081 ggcgaaagca gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa tggcggagaa
     7141 attgcagggt ttcaacgtgt tgagctacct ctcgtcgcat gatacccgtc tgttccgtga
     7201 agggggcgac aaagcagcag agttattact attagcgcca ggcgcggtac aaatctttta
     7261 tggcgatgaa tcctcgcgtc cgttcggtcc tacaggttct gatccgctgc aaggtacacg
     7321 ttcggatatg aactggcagg atgttagcgg taaatctgcc gccaacgtcg cgcactggca
     7381 gaaaatcagc cagttccgcg cccgccatcc cgcaattggc gcgggcaaac aaacgacact
     7441 ttcgctgaag cagggctacg gctttgttcg tgagcatggc gacgataaag tgctggtcat
     7501 ctgggctggg caacagtgac ttttccggct tcccgttcgt cagtacctcg ggaagccgcc
     7561 aaccaggata aaatgtcagc cctaatcagc gttgcgggat aaagcaccgc tcactcttca
     7621 acagaccgat ttgcacccca gcaaatgtag cgttattgtt accttccttg ctacagagtt
     7681 cgacagatat cccgctatga cattctccct ttttggtgac aaatttaccc gccactccgg
     7741 cattacgctg ctgatggaag atctgaacga cggtttacgc acgcctggcg cgattatgct
     7801 cggcggcggt aatccggcgc agatcccgga aatgcaggac tacttccaga cgctactgac
     7861 cgacatgctg gaaagtggca aagcgactga tgcactgtgt aactacgacg gtccacaggg
     7921 gaaaacggag ctactcacac tgcttgccgg aatgctgcgc gagaagttgg gttgggatat
     7981 cgaaccacag aatattgcac taacaaacgg cagccagagc gcgtttttct acttatttaa
     8041 cctgtttgcc ggacgccgtg ccaatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc
     8101 ggaatacatt ggctacgctg acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc
     8161 gaatattgaa ctgctgccgg aaggccagtt taaataccac gtcgattttg agcatctgca
     8221 tattggcgaa gaaaccggga tgatttgcgt ctcccggccg acgaatccaa caggcaatgt
     8281 gattactgac gaagagttgc tgaagcttga cgcgctggcg aatcaacacg gcattccgct
     8341 ggtgattgat aacgcttatg gcgtcccgtt cccgggtatc atcttcagtg aagcgcgccc
     8401 gctatggaat ccgaatatcg tgctgtgcat gagtctttcc aagctggggc tgcctggctc
     8461 ccgctgcggc attatcatcg ccaatgaaaa aatcatcact gccatcacca atatgaacgg
     8521 cattatcagc ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg
     8581 taacgatctg ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca
     8641 ggaaactatc gccatcattc gccgctattt accggaaaat cgctgcctga ttcataaacc
     8701 ggaaggagcc attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct
     8761 ctatcagcgc ctgaaagcac gcggcgtgct gatggtgccg gggcacaact tcttcccggg
     8821 actggataaa ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc
     8881 ggagaaaatt gaggcggggg tgaagattct ggcggaagag atagaaagag cctgggctga
     8941 aagtcactaa aaattgcctg atgcgctacg cttatcaggc ctacatgatt tctgcaatat
     9001 attgaatttg gaagaatttg taggccggat aaggcgttta cgccgcatct ggcataaaca
     9061 aagcgcacta ttccggcgtt gagaaacgcc ggaatacgtt tcaaacaggc tgctgctgta
     9121 gccgatgccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt
     9181 caacacaggc cggccccgtc tcccgatgcc agcacagatc gcacttaatc gccctcgcct
     9241 ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata cagcttttac
     9301 agccaatgca acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa
     9361 cagggcagac attcgcgcac ggtgcatctt cacactgatg acaggctact ggcgtggtcc
     9421 agctgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac aacgcagcgc
     9481 aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg caaccgatac
     9541 atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac taaaacagca
     9601 gagtgccaga gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt aaagttttgg
     9661 cacttgtcca aagcgttatg tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc
     9721 attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct ctcccacctg
     9781 tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc catgaatatc
     9841 aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt ccatcgccgc
     9901 tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt
     9961 gcgggttaac ggctggatct catgctgatg gttttcccag tatgacttca cgtagtccgg
    10021 gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca tatgctggcc
    10081 aatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa tcaaaatagc
    10141 gtgatcgtct tcgcggctgg agaagttaat ggtttcacca gtggcgatgt tcagtgcctc
    10201 aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct gcccgacggc
    10261 aataaatttg gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca catagccgca
    10321 ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag ccagctccga
    10381 aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca gcatcaaccc
    10441 gcgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct cgttctcttt
    10501 ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt
    10561 agttcacgat ctcgacagat aatttataac cacttgattt ttatgtcttt tgaaattcat
    10621 caatcagatt gctttgttaa aaagtgatcg atatatttga aatctagttt cgtatattga
    10681 aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg
    10741 agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac agcgaaacgg
    10801 ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg
    10861 ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt cctgatgccc
    10921 aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg
    10981 gcaacctgac ggcgaaaaag atgatggatc gcgccattga actggctgcc gatcacggta
    11041 ttggtctggt ggcacttcgt aatgccaacc actggatgcg cggcggcagc tacggctggc
    11101 aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc gtaatgccgc
    11161 cgtggggcgc aaaagagtgt cgcatcggca ctaacccgct gatcgtcgcc attccttcca
    11221 cgccgatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg ttagaagtta
    11281 accgcctggc aggccgtcag ctcccggtcg atggtggctt tgatgatgag ggcaatttga
    11341 ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc tactggaaag
    11401 gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac ggcgcatcgg
    11461 ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt tttattgcca
    11521 ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa cgtatcatgg
    11581 attacgttac taccgctgaa cgcgctgacg aaaaccaggc catccgctta cccggccacg
    11641 aatttactac cctgctggcc gaaaaccgcc gtaacggcat cactgttgat gacagcgtgt
    11701 gggccaaaat ccaggcgtta tgaggagata agtcatgatt tttggacata tcgcgcagcc
    11761 caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc gcgctaccaa
    11821 cttcaacgtc ctagagccgg gcgttgtcga aatcgacggc aagaatattt atgcgcaaat
    11881 tatcgattta accacacgcg aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat
    11941 cgatattcag tttctggcgt ggggtcaaga gaaaatcggc attgctattg atacaggaaa
    12001 taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc atgacagtga
    12061 acatgaatca tttatagaaa tgataccggg cagctacgct attttcttcc cgcaggatgt
    12121 tcaccgacca ggttgtattc tgcaaacagc ctctgagata cgaaaaattg tggttaaagt
    12181 cgcattaaca gcgctgaatt aatcaaataa tcgtctacgc cagaacgact gggtaatgt
//
LOCUS       DADKXM010000115        12156 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.116, whole genome shotgun sequence.
ACCESSION   DADKXM010000115 DADKXM010000000
VERSION     DADKXM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.116"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            166..255
                     /gene="kdpF"
                     /locus_tag="JQR05_003088"
     CDS             166..255
                     /gene="kdpF"
                     /locus_tag="JQR05_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY5325186.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            255..1928
                     /gene="kdpA"
                     /locus_tag="JQR05_003089"
     CDS             255..1928
                     /gene="kdpA"
                     /locus_tag="JQR05_003089"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY5325187.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAELFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1951..3998
                     /gene="kdpB"
                     /locus_tag="JQR05_003090"
                     /pseudo
     CDS             1951..3998
                     /gene="kdpB"
                     /locus_tag="JQR05_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4007..4579
                     /locus_tag="JQR05_003091"
     CDS             4007..4579
                     /locus_tag="JQR05_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY5325188.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4572..7259
                     /gene="kdbD"
                     /locus_tag="JQR05_003092"
                     /pseudo
     CDS             4572..7259
                     /gene="kdbD"
                     /locus_tag="JQR05_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            7256..7933
                     /gene="kdpE"
                     /locus_tag="JQR05_003093"
     CDS             7256..7933
                     /gene="kdpE"
                     /locus_tag="JQR05_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY5325189.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8208..8312
                     /locus_tag="JQR05_003094"
     CDS             8208..8312
                     /locus_tag="JQR05_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY5325190.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8623..10810
                     /gene="speF"
                     /locus_tag="JQR05_003095"
                     /pseudo
     CDS             8623..10810
                     /gene="speF"
                     /locus_tag="JQR05_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            10807..12126
                     /gene="potE"
                     /locus_tag="JQR05_003096"
     CDS             10807..12126
                     /gene="potE"
                     /locus_tag="JQR05_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY5325191.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSTTLYVDSWNPHHAPFFSTVGSSIAMTLWAFLGLESACANTDVVEN
                     SERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
BASE COUNT         2685 a         3050 c         3382 g         3039 t
ORIGIN      
        1 ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat catggccttt
       61 gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct ttgcagccag
      121 aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag tgcaggcgtg
      181 atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc cctgatcaat
      241 gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt ttactggtat
      301 taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat gacattcctc
      361 ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc tctgaccatg
      421 agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg gggctggcgg
      481 agctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag cagttgccgg
      541 ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat accaactggc
      601 aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc ttaacggtac
      661 aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc cgtgcgttta
      721 ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc atcacgttat
      781 gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa ggtgcgctgc
      841 aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag ctgttaccca
      901 tgggaccagt agcttctcaa gaagcgatca agatgctcgg tactaacggc ggtggcttct
      961 ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac ttcgtgcaga
     1021 tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa gtgacgggcg
     1081 atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc atctgcgtag
     1141 gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc ggcgcagaca
     1201 gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt agcctgtttg
     1261 cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat tcgtttaccg
     1321 ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg ttcggcggtg
     1381 tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt attgccgggc
     1441 tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc gagatgaaac
     1501 tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg gcgttggcga
     1561 tgatgaccga cgccggacgt agcgccatgc ttaaccctgg cccgcatggt tttagcgaag
     1621 tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc ggattaagcg
     1681 ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt cgcttcgggg
     1741 tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc caacccgcca
     1801 gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc ggcaccgtgt
     1861 tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg gcggaatatc
     1921 tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc gctattcgaa
     1981 ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc gcaggcgcaa
     2041 tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac ctgtattagc
     2101 atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc aattagcggc
     2161 tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga aggccgcagt
     2221 aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg caagctgcgt
     2281 gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg taaaggtgac
     2341 atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat tgaaggcggc
     2401 gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg tgaatccggc
     2461 ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct ggtgattgag
     2521 tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt ggaaggcgca
     2581 cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc tctgaccatc
     2641 gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg taatgccgtg
     2701 agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat tggtggcctg
     2761 ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt gattgccacc
     2821 agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga taaaaccggc
     2881 accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg cgtggatgaa
     2941 aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc ggaaggccgc
     3001 agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt gcagtcgctc
     3061 catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa catcgacaac
     3121 cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc taacggcggt
     3181 cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg agccacgccg
     3241 ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga tatcgtcaaa
     3301 ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg gtgatgatta
     3361 ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc gatgattttc
     3421 tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag gcggaaggtc
     3481 gtttggtagc gatgaccggc gacggcacca acgatgctcc ggcgctggcg caggcagatg
     3541 tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat atggtggatc
     3601 tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag atgctgatga
     3661 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     3721 ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc atgcgcctgc
     3781 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     3841 tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     3901 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     3961 tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga gtggattacg
     4021 tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt acccgctgct
     4081 gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt tgattcgtga
     4141 aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca acggctattt
     4201 tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt ctggcgggag
     4261 caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg ttgccgcatt
     4321 acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga cggcgtcggc
     4381 aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcatcgc
     4441 aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat acagccaaca
     4501 accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcact
     4561 ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg ctggaacaaa
     4621 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca ggcgtcggga
     4681 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     4741 ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa gggctggctg
     4801 ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt gatctcgatg
     4861 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     4921 caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa gctggcattg
     4981 atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     5041 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5101 tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5161 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5221 ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct
     5281 ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca
     5341 tcggccataa caccggcagc gaaaaactgg tccgcgcggc ggcgcggctg gcgtcacggc
     5401 tggggagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga
     5461 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5521 caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt gaacataatc
     5581 tcggcaagat tattctcggt cgcccagcct cgcgccgctg gtggcgtcgg gaaacatttg
     5641 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     5701 cacccgcccg cacgattaac aacgcgccgg atagccgctc atttaaagac aagtggcggg
     5761 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     5821 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtgg
     5881 tggtggtggc gctattttat ggacgctggc cttcggtggt tgccaccgtc attaatgtgg
     5941 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     6001 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg
     6061 gcgtgcgtta tcaggcgcgg gtagcccgtt accgcgcctg ataacgcaca cggcacttat
     6121 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6181 aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     6241 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     6301 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     6361 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc
     6421 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc
     6481 tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga
     6541 tggcaagcga acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac
     6601 gcacgccgct tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg
     6661 aaggatcacc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc
     6721 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
     6781 aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt
     6841 tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc
     6901 cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc
     6961 aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg
     7021 ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg
     7081 ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg
     7141 tggatgtaca cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc
     7201 gtgttacact tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac
     7261 aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga
     7321 gggcgacggg atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc
     7381 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga
     7441 gtttatccgc gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag
     7501 cgaagagagc gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
     7561 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac
     7621 cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt
     7681 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt
     7741 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     7801 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     7861 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg
     7921 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     7981 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8041 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8101 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8161 gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata
     8221 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata
     8281 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     8341 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     8401 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     8461 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     8521 tttatttatc agcggtggag ctttgctttt tttccggcgt gatcgatttc tcctttgaga
     8581 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     8641 gccgttaacc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     8701 gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
     8761 aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
     8821 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
     8881 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa
     8941 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
     9001 gactgcccgg gccatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc
     9061 gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg
     9121 ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     9181 tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagcggtt
     9241 ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct
     9301 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt
     9361 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
     9421 gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct
     9481 gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
     9541 attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt
     9601 atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt
     9661 attctggtta cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc
     9721 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
     9781 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
     9841 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
     9901 aatggcatta atgcccgcaa actgatcctc gataactgtc atcatattcg tccgttcgta
     9961 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
    10021 ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag
    10081 aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
    10141 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgtagt
    10201 gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc
    10261 caaacttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc
    10321 gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta
    10381 taccctgcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca
    10441 actgcaaaaa gagatgttcc gcaaagcgca cttcccacac gtcagcatga atccgcaaga
    10501 agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgccgaagg
    10561 tcgtatcgct gccgaagggg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
    10621 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
    10681 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
    10741 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga
    10801 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
    10861 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
    10921 acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
    10981 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta tgccgagtat
    11041 gctttcggta aatccggtaa ctttatggcg aactatacct acggcgtctc actgctgatt
    11101 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
    11161 ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct
    11221 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttaccgtgtg ggggggcatt
    11281 attccggtgg taggcctgtg cattatcggc tggttctggt ttagcacgac gctttacgtt
    11341 gattcctgga atccgcatca tgcaccgttc ttcagtacag taggttcttc catcgctatg
    11401 acgctgtggg cttttcttgg tctggagtct gcgtgtgcga atactgatgt agtggaaaac
    11461 tcggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat
    11521 atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc aaattcaacg
    11581 gcaccatttg gcctggcctt cgcacagatg ttcacaccag aagtgggtaa agtcattatg
    11641 gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag
    11701 gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa
    11761 gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg
    11821 atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg
    11881 accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct
    11941 aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtat
    12001 agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc
    12061 ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac
    12121 ggttaaaaaa gcaaagtaaa aaagggcgat cttgcg
//
LOCUS       DADKXM010000116        11864 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.117, whole genome shotgun sequence.
ACCESSION   DADKXM010000116 DADKXM010000000
VERSION     DADKXM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.117"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..658)
                     /gene="hpaA"
                     /locus_tag="JQR05_003097"
     CDS             complement(<1..658)
                     /gene="hpaA"
                     /locus_tag="JQR05_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY5325192.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     T"
     gene            complement(668..2022)
                     /gene="hpaX"
                     /locus_tag="JQR05_003098"
                     /pseudo
     CDS             complement(668..2022)
                     /gene="hpaX"
                     /locus_tag="JQR05_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
     gene            complement(2044..2787)
                     /locus_tag="JQR05_003099"
     CDS             complement(2044..2787)
                     /locus_tag="JQR05_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY5325193.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGLADLSADMGYAGNPQHPEVQAANEQLAKRYLELGALFVAVGVDTTL
                     LARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(2798..3601)
                     /gene="hpaH"
                     /locus_tag="JQR05_003100"
     CDS             complement(2798..3601)
                     /gene="hpaH"
                     /locus_tag="JQR05_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY5325194.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKTAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(3669..4049)
                     /locus_tag="JQR05_003101"
     CDS             complement(3669..4049)
                     /locus_tag="JQR05_003101"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY5325195.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(4059..4910)
                     /gene="hpaD"
                     /locus_tag="JQR05_003102"
     CDS             complement(4059..4910)
                     /gene="hpaD"
                     /locus_tag="JQR05_003102"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY5325196.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(4912..6378)
                     /gene="hpaE"
                     /locus_tag="JQR05_003103"
     CDS             complement(4912..6378)
                     /gene="hpaE"
                     /locus_tag="JQR05_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY5325197.1"
                     /translation="MKKVNHWINGKNVADNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTTTGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(6375..7664)
                     /locus_tag="JQR05_003104"
     CDS             complement(6375..7664)
                     /locus_tag="JQR05_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY5325198.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            7936..8382
                     /gene="hpaR"
                     /locus_tag="JQR05_003105"
     CDS             7936..8382
                     /gene="hpaR"
                     /locus_tag="JQR05_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY5325199.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            8501..10165
                     /gene="tsr"
                     /locus_tag="JQR05_003106"
     CDS             8501..10165
                     /gene="tsr"
                     /locus_tag="JQR05_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY5325200.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPCQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(10214..11575)
                     /locus_tag="JQR05_003107"
     CDS             complement(10214..11575)
                     /locus_tag="JQR05_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325201.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSIRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
BASE COUNT         2844 a         3052 c         3145 g         2823 t
ORIGIN      
        1 ttgttgcata ttcagggacc attaaatgtt ggcgataatt ttcgtcaatc agccgattaa
       61 acttctggaa taaacacatc tcgccgcgca cgctgcatgt tgaaatatca tttggtggtg
      121 cttcacgcag taggattgta aaaatacttt gcgccagtga ttgcaataga atttcccgtc
      181 ctggcttatt ttgttgaaac tccagggata ttatcggcca ataatgattt aatgccgcca
      241 gtgtatgttc cattccctcc agtgacagac agataccttg tagcattgcg gcatctccac
      301 taccaggcca gagcttttct accaggggcc aaattaactc ctgacgtacg gtcagaacat
      361 gcccatcgct atcaggatca gtaaagaaag tatgtggcac cgatggtggt gttagtacaa
      421 aaagcggtgc gcgcacagaa tagaagtgct catccagttg taaagtaatg cgtccagtga
      481 ccagataatg gagttgaaaa tatctatcgt gccagtgagg gcgcatatcg cgtccaaaga
      541 aattcgctaa ccgggcaaat gtttcataat gcacatcttc accagcgtag cgggcatcat
      601 attcccggac caaatcaata ttttcataac catgttcaaa gatagcttca ctggacatca
      661 tgactcctta ttgcggtttt ggtgcgataa gttcagatga aagagagtgt tcccgactcc
      721 ccatagggat tcgggtcatg actaacgcgc ccagtaccag caatcctgct acaaaccaca
      781 aacctgagct aaaactgccg gttgcatctt tcaaaatacc gataagtaat ggactgactg
      841 ctgaacctac attgccgaca gcattgatga cagccacgca acggcacgcg attgcaagct
      901 tataacttga tccggtgttg tccaaaagat agccatcgcg gtaaatgatc ccattgaggc
      961 cataatgatg ccgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     1021 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     1081 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     1141 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     1201 attaatcgca ctcagagtat tggtcaggca aaaataagca agtgtatata gcatgatggc
     1261 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     1321 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     1381 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     1441 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     1501 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     1561 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     1621 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     1681 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     1741 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     1801 cgtgacataa aagagtgtcg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     1861 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
     1921 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
     1981 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
     2041 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
     2101 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
     2161 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcgg cctgtacttc
     2221 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgcca ggccgataaa
     2281 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
     2341 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
     2401 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
     2461 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
     2521 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
     2581 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
     2641 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctgctc cggccagtaa
     2701 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
     2761 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
     2821 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggcggg
     2881 tgaacgaacc accgagaatg atttgcccgg cttccagttg tacgtcatac ggtgcgagtt
     2941 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
     3001 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
     3061 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
     3121 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
     3181 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
     3241 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
     3301 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
     3361 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
     3421 tgtggccttt cagcgtgcga ccttcggcgg ttttcaggcg aacccattcg cgctgcacgg
     3481 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
     3541 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
     3601 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
     3661 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
     3721 agctctgcaa tctcaaatga aagcgccagg taacggctct ccatcagtga ggcgaagtgc
     3781 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
     3841 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
     3901 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
     3961 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
     4021 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
     4081 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
     4141 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
     4201 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
     4261 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
     4321 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
     4381 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
     4441 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
     4501 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
     4561 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
     4621 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
     4681 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
     4741 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
     4801 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
     4861 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
     4921 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
     4981 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
     5041 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
     5101 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
     5161 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
     5221 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
     5281 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
     5341 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
     5401 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
     5461 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgttg
     5521 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
     5581 ggcggcgtcc agcgcgcgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
     5641 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggt
     5701 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
     5761 ggttgcgccg tagccctgta ctacgtttag cacgcccgcc ggaataccag cttccagcgc
     5821 cagctcaccc aggcggttgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
     5881 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
     5941 cggcgacacc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
     6001 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
     6061 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
     6121 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
     6181 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
     6241 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
     6301 cgttgccgga ttggtagtct ggaagtagtc gttatctgca acatttttgc cgttgatcca
     6361 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
     6421 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
     6481 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
     6541 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
     6601 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
     6661 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
     6721 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
     6781 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
     6841 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
     6901 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
     6961 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
     7021 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
     7081 ccttctgcga gaacacgcac gcgatcgcct ggctgtattt ccacgcgcgc ctgtggcgtg
     7141 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
     7201 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
     7261 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
     7321 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
     7381 acgtcgttag ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
     7441 gtcgccgttt tccccacaat cagagcaaca gtcgcaccgc tcagtacttt ttcaccctgt
     7501 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
     7561 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca agcatcaagt
     7621 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
     7681 cgatcatctt aatcattaat atgttaatga ttacttttat tcccgattgc gtagtttggc
     7741 aaaagtaagt gatataaatg tgatctaatt aacaaactat ttacatttaa agataataaa
     7801 atattaaata tcagtaagtt aataaaatat catcaatttt gataaaatga agaattgcgg
     7861 gggcagctca gtcccggtaa cgtgatgatt aattgtttct atattaatga ttttgaattt
     7921 ttcaggggaa agataatgca cgactcacta accattgcat tgctccaggc gcgagaagcg
     7981 gcgatgagtt attttcgccc aatcgttaaa cggcataatt tgactgagca acagtggcgc
     8041 atcgtgcgta ttctggcgga aagcccatca atggattttc acgatctggc gtatcgcgcc
     8101 tgcattttgc gcccaagcct gaccgggatc ctcacgcgga tggaacgcga cggtttagtg
     8161 ttgcgattaa agccgatcaa tgatcagcgg aagctctata tctcgctgac caaagagggg
     8221 caggcgctgt ataaccgcgc tcagacacag attgaagaag cctatcggca gattgaagct
     8281 cagtttactg ccgagaaaat gcaacagtta acccatctgt tagaggaatt tattgctctg
     8341 ggcaattccc gacaagaaga catcccgggc gataatgaat aactttttag tatctgataa
     8401 ccagcgcctg gtttttccat ggatggcggg ttacatcttt tcataaagtt tttgctttcc
     8461 aggccgaaaa tcttgcatcg gtccacagga aagagaaacc atgttaaaac gtatcaagat
     8521 cgtgaccagc ttactgctgg ttttggccgt ttttggcctt ttacaactga catcaggcgg
     8581 tctgtttttt aatgccttaa agaatgacaa agaaaatttc actgttttac aaaccattcg
     8641 ccagcagcaa tccacgctga atggcagctg ggtcgcgttg ttgcagacgc gtaacaccct
     8701 caaccgcgcg ggtatccgct acatgatgga tcagaataat attggtagcg gttcaaccgt
     8761 tgctgagctg atgcagagtg ccagtatttc gctgaaacag gcggaaaaaa actgggcgga
     8821 ttacgaagcg ttgccgcgtg acccgtgtca gagcaccgcc gcagcggcag agatcaaacg
     8881 taattacgat atttatcaca atgcgctggc ggagctgatc caactgttag gtgcaggcaa
     8941 aatcaacgag ttctttgatc agccgaccca gggatatcag gacggtttcg agaagcagta
     9001 tgtggcttac atggagcaaa acgatcggct ctatgatatc gccgtcagcg ataacaatgc
     9061 ctcctacagc caggcgatgt ggattctggt gggcgtgatg atcgtcgtac tggcggtcat
     9121 cttcgccgtc tggtttggta ttaaagcctc gctggtagcg ccaatgaatc gcctgattga
     9181 cagcattcgt catattgcag gcggcgatct ggtgaaaccg attgaggtgg atggctctaa
     9241 tgagatgggg caactggcag agagtttgcg ccatatgcag ggagagctga tgcgtaccgt
     9301 cggtgatgtg cgcaacgggg ccaatgccat ctatagcggt gccagcgaaa tcgctaccgg
     9361 caataatgat ctctcttcgc gcaccgagca acaggccgct tcgctggaag agacggcagc
     9421 cagcatggag caactgaccg caacggtgaa acagaacgcc gagaatgcgc gccaggccag
     9481 ccatctggca ttaagtgctt ctgaaacggc gcaacgcggc ggcaaagtgg tagataacgt
     9541 ggtgcagact atgcgcgata tctccaccag ttcgcagaaa atcgccgata ttatcagcgt
     9601 aattgacggc attaccttcc agaccaatat tctggctttg aacgcggcgg ttgaagcagc
     9661 gcgtgcgggt gagcaaggac gtggttttgc ggtggttgcg ggagaagtgc gtaatctggc
     9721 ccagcgtagc gcccaggcgg ctcgtgaaat taaaagcctg attgaagact cggtggggaa
     9781 agtggatgtt ggctctacgc tggtcgaaag cgccggggaa acaatggcgg agattgtcag
     9841 tgctgtgacc cgcgtgacgg acattatggg cgaaatagct tctgcttctg atgagcagag
     9901 ccgtggtatc gatcaggttg gcttagcggt tgctgagatg gaccgggtaa ctcaacagaa
     9961 cgccgcgctg gtggaagaat ctgccgctgc cgccgccgcg ctggaagagc aggccagtcg
    10021 cctgaccgaa gctgtggcag tgttccggat tcagcaacag cagcaacagc agcgtgaaac
    10081 atcggctgtg gtaaaaaccg tgacgccagc tacgccgcgt aaaatggcag tggcagatag
    10141 cggggagaac tgggaaacgt tttaatcgcc ataaaaatgc ccgataagca aaatgttatc
    10201 gggcataggg agtttaatct ttacgcgggt cgttgatcgg ctggcgaacc aggaagatgt
    10261 acgccagtgc tcccgctgcg gtgacgcaac cgcaaatgat tagtgccaga cggaatgagt
    10321 gggttgtatc aacaataaaa ccagtaatga tcggcgcgaa agaggcgcag atgaagctgg
    10381 caaagttctg gatactgccc accgacgcag tcatgcgaga agcaactgcg acgtggatca
    10441 agccccagca ggatgttccg gcaaagtgaa tacagaacag tgccatgcca atcagcagaa
    10501 ccgccatcat ggatgttgtc gcttgtggta ctatcagcgt aaaggcggca gaacagaaca
    10561 tcccggtaat aatgcagatc ttacggcttt taatcggagc cattccccct ttgaccagcc
    10621 agtcagtaac gtaaccgttg accagcatcc cggcagcccc aaacaggaaa gggatagccg
    10681 ccatcaaccc tgtgcttttt aaatccaggt tataggctgt ttgcaggtaa ccaggaagcc
    10741 aggccagata cagccacgca gtgtagttga tgccactgaa tccgagcatc attccccaca
    10801 ttgtgcggtt acggaacagg ctgcgccatt cggcaaaatt gagcggatct cggcgggcat
    10861 tgacgctacc tgcattgaga taagcttgtt caacggcagt cagttctacg tgctcgcggt
    10921 tgcgatagag catataccag ccgatggcga gaaaaatccc cagtacacca atggtaataa
    10981 acatcccgcg ccagcccatc accagcatca ttgccgccag aatcggtggg cttacagcaa
    11041 cgccaatggt agaagctgcg ttgaagaagc ccatcgggcg tccgcgctct ttgatgttga
    11101 accagtcgtt aatgactttt acaccgcatg ggttcatcgg cgcttcacca atccccatac
    11161 cgatacgcac caacacgaac tgcgtaaagc tgtgcaccat gccagacatg gcctggaaca
    11221 gtgaccagaa gaacatcccc agtcccagca tcaggcgtgg gcctttacga tccaatagtg
    11281 ggccgcaagg aagttgcgca atgccgtaag cgagtgaaaa cacggagagc aaagcgccga
    11341 tttcggtggc acttaatccc aattcttcac gaatcgttaa atttgctacc gacagcgaac
    11401 tgcggtcgag ataattgatt accgccgcaa aaaataataa taacatggcg gtggtttgaa
    11461 tgcgtttaat tcggatactg cgttgaacta atccatctgg tggcacggga atatctgccg
    11521 acgaagatgg agtaaatgaa gaacgcggat cgagggtgat attttctttt tccacgccgg
    11581 actcctgtat ttataatttt aattgtttac gtaattgtgc attacgcaag gttgacatta
    11641 acggcaggtt aaagttttag gactttacta ccgctcacaa aaacattttt agtcgactga
    11701 ttgtgagcat aggtaagcgg gaattttccg gtatttatct tttgataaaa ccgtgcaccc
    11761 gctaatagtt tgctgttttt aatccgtact taatgagcat aacgcgtgtt ctcattaatg
    11821 gatcgaatca ttgattgttt agctgaattc aaatggttac ggag
//
LOCUS       DADKXM010000117        11712 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.118, whole genome shotgun sequence.
ACCESSION   DADKXM010000117 DADKXM010000000
VERSION     DADKXM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.118"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JQR05_003108"
     CDS             complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JQR05_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY5325202.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEVERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTAL"
     gene            1519..2207
                     /gene="cusR"
                     /locus_tag="JQR05_003109"
                     /pseudo
     CDS             1519..2207
                     /gene="cusR"
                     /locus_tag="JQR05_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
     gene            2197..3639
                     /gene="cusS"
                     /locus_tag="JQR05_003110"
     CDS             2197..3639
                     /gene="cusS"
                     /locus_tag="JQR05_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY5325203.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKCEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3789..6026
                     /gene="nrfB"
                     /locus_tag="JQR05_003111"
                     /pseudo
     CDS             3789..6026
                     /gene="nrfB"
                     /locus_tag="JQR05_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6013..8985
                     /gene="nfrA"
                     /locus_tag="JQR05_003112"
                     /pseudo
     CDS             6013..8985
                     /gene="nfrA"
                     /locus_tag="JQR05_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8986..9876
                     /locus_tag="JQR05_003113"
     CDS             8986..9876
                     /locus_tag="JQR05_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY5325204.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10059..10820
                     /locus_tag="JQR05_003114"
     CDS             10059..10820
                     /locus_tag="JQR05_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5325205.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPRLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10866..10942)
                     /locus_tag="JQR05_003115"
     tRNA            complement(10866..10942)
                     /locus_tag="JQR05_003115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10906..10908),aa:Arg,seq:tct)
     gene            11185..>11712
                     /gene="fimZ"
                     /locus_tag="JQR05_003116"
     CDS             11185..>11712
                     /gene="fimZ"
                     /locus_tag="JQR05_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY5325206.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSNKTVSAHKSNIYG"
BASE COUNT         2886 a         3018 c         2996 g         2812 t
ORIGIN      
        1 taaggcggta tacaaataaa tttcgttaac ctgacgggca taattcaggt caagtaaagt
       61 ttgtcgggtt gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc
      121 gtgctgatat aatgcccgcg cccgttgcag ggtaatttgc agcgacgcca gataacgctg
      181 ctgggcgctg atttgatcgt ccaggctttg acgtaatgcc agcgcatctg ccacctcttt
      241 aaaggcgttc tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc
      301 gatatccaga ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat
      361 aaaattccac atcccgctgc tagcgttaaa taatgatgac agatcgctgc tggcggttga
      421 tattccgctg gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc
      481 attagccgcc attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg
      541 cgacgacaag cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc
      601 ttgcggtagc ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag
      661 ttccccctga cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc
      721 cagcgccaga acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg
      781 ctgataatta cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt
      841 gaaatagctt tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc
      901 agtggctaaa taattttggc gctcgacttc gctcatgttc tttaagcgac cgaaaaaatc
      961 gagatcaaag ctggcgttaa ggccagtcga gaactcccgc gtcgtggctg aatcgccttt
     1021 aagattgccg ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc
     1081 ggtcagacga tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg
     1141 gttattcacc agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg
     1201 ccagcccgcg ttctggtagt tatccgctgc gttaaccagg ccgttctggc tgagtgagaa
     1261 ctgctgcggt acgggcattg ccggacgctg ataatccggt gccagtgaac aaccagttag
     1321 cgcaagggcc acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt
     1381 gattttatgt cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca
     1441 aaattgtcat tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg
     1501 gcaacatgcg gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa
     1561 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg
     1621 aatggctacc atctggcgat gaccggtgat tatgatctga taatcctcga tattatgctg
     1681 ccagacgtga acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg
     1741 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg
     1801 gcagatgact acctggtgaa accattcgct tttgctgaac tgctggcgcg ggtgcgcact
     1861 ctcctgcggc gcggggcggg gcggcggtga ttatcgaaag tcagtttcag gttgccgatc
     1921 tgatggtcga tctcgtcagc cgcaaagtca cccgcagcgg cacgcgcatc actctgacca
     1981 gtaaagaatt tactctgctg gagttttttc tccgccatca gggcgaagtg ctgccccgct
     2041 cgcttatcgc ctcgcaggta tgggacatga atttcgacag cgacactaac gccattgatg
     2101 tggcggtgaa gcggctacgc ggcaaaatcg acaacgactt tgagccgaag ctgattcaaa
     2161 ccgtgcgcgg cgtgggttac atgcttgagg tgccggatgg tcagtaagcc atttcagcgc
     2221 ccgttttcgc tggcaacccg cctgaccttt tttatcagcc tggccaccat cgcggcgttt
     2281 ttcgcctttg catggatcat gatccactca gtaaaagtgc attttgccga gcaggacatt
     2341 aatgatttaa aagagattag cgccaccctt gaacgggtac taaatcaccc tgacgaaacg
     2401 caagcccgac gcttaatgac gctggaagat atcgtcagtg gttattccaa cgtgttgatt
     2461 tccctggcag atagccacgg caaaacggtg tatcactccc ccggtgcgcc tgatatccgc
     2521 gagtttgcac gtgatgccat acccgataaa gacgcgcggg gtggcgaagt gtatctcctt
     2581 tccggcccga cgattatgat gccaggccac ggtcacgggc atatggaaca cagcaactgg
     2641 cggatgatta acttgccggt tggcccgttg gtggacggca aaccgattta tacgctctac
     2701 atcgcgctgt cgatcgattt tcatcttcat tacataaatg atttgatgaa taaacttatt
     2761 atgaccgcat cggtaatcag catcctgatc gtctttatcg tgctgttggc ggtacataaa
     2821 ggtcacgcgc cgatccgcag cgtcagccgt caaatccaga atatcacctc gaaagatctc
     2881 gacgttcgcc ttgacccgca gaccgtgccg attgagctgg aacagctggt actgtcgttc
     2941 aaccatatga tcgagcgtat tgaggatgtc tttactcgcc agtccaattt ctcagcggat
     3001 atcgcccacg aaattcgcac accaattact aatctcatta cgcaaacgga aatcgccctc
     3061 agccagtcgc gcagccagaa ggagctggaa gatgtgctct actctaatct cgaagagctg
     3121 acgcgaatgg cgaaaatggt cagcgatatg ctgtttctcg ctcaggccga taacaaccag
     3181 ctaatccccg aaaagaaaat gctcaacctg gcggatgaag tcggcaaagt gttcgatttt
     3241 ttcgaggcgc tagcggaaga tcgcggcgtg gagttgcggt ttgttggcga caaacgtcag
     3301 gttgcgggcg atccgttgat gctgcgtcgg gcgctaagca acctgctttc taatgccctg
     3361 cgttatacgc caaccagaga gacaattgta gtgcgctgtc agacggtcga tcaccaggtg
     3421 caagtttccg tcgaaaaccc cggtacgccc attgcgcccg agcacttacc gcgattgttt
     3481 gaccgtttct atcgcgttga cccctcccgc cagcgaaaat gtgaaggtag cggtattggg
     3541 ctggcgatag tgaaatcgat tgttgtcgcg cataaaggca cggttgcggt aacgtcagat
     3601 gcgcggggga caaggtttgt tatcacatta cccgcttaat tattcagcaa acggcaactt
     3661 ttataaccag tgtaaaaata acgtgccgca atagtcatac ctattaatgg taaaaagctg
     3721 tcacaattca taaaaaacct taatatacgc caccctaaac ataaccagcg ttaatgtaag
     3781 gtttttgtgt ggactggctt cttgatgttt ttgctacctg gctctacggc ttaaaagtaa
     3841 tcgcgataac gttagcggtc atcatgttca tcagcgggct ggacgatttt tttattgatg
     3901 tcgtctactg ggtacgccgc attaaacgca agttgagtgt ttatcgccgc tacccgcgaa
     3961 tgagttaccg cgaactgtat aaaccagatg aaaaaccgtt agcgattatg gttccggcgt
     4021 ggaatgaaac gggcgtcatc ggcaatatgg ccgagctggc agcgaccacg ctcgactacg
     4081 aaaactatca tatctttgtt ggcacctacc ccaacgaccc cgatactcaa cgtgatgttg
     4141 acgaagtgtg cgctcgcttt ccgaacgtgc ataaggtagt ctgcgcgcgt cctggtccca
     4201 ccagcaaagc cgactgtctg aacaacgtgc tggacgccat cacccagttt gaacgtagcg
     4261 ccaatttcgc ttttgctggt tttattctgc atgacgccga agatgtgatt tcaccgatgg
     4321 aattgcgtct gttcaactat ctggtcgagc gtaaagatct gattcagatc ccagtgtatc
     4381 cgtttgaacg cgaatggacg cacttcacca gcatgactta cattgatgag ttttcagaac
     4441 tgcatggcaa agatgttccg gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg
     4501 tcggcacctg tttcagccgc cgcgccgtga ccgcactgtt agctgacggt aacggtattg
     4561 ctttcgacgt gcagagtctt actgaagatt acgacattgg cttccgcctg aaagaaaaag
     4621 gtatgacgga aatttttgtc cgttttccgg tggtggacga agccaaagaa cgcgagcagc
     4681 gtaaattttt acagcacgca cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg
     4741 ataccttttc gactgcggtt cgacaaaaat ctcgctggat catcggcatt gttttccagg
     4801 gctttaaaac ccataaatgg acctccagcc tgacgctgaa ctactttctc tggcgcgacc
     4861 gcaaaggggc aatcagtaac tttgtcagct tcctcgcgat gctggtgatg atccagcttt
     4921 tgctgttgct ggcgtatgaa agtttgtggc ccgatgcctg gcatttcctt tctattttta
     4981 gcggcagcgc atggttaatg accctgctgt ggctaaactt tggcttgatg gttaaccgca
     5041 tcgtgcagcg agtgattttc gtcactggct actacggcct cacgcagggc ctgctttccg
     5101 tcctgcgtct tttctggggc aacctgatta actttatggc taactggcgc gtgctgaaac
     5161 aggttcttca gcacggcgat ccgcgtcgcg tggcgtggga taaaacaacg catgacttcc
     5221 ccagcgtcac tggcgatacc cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc
     5281 aggtcatcac tgaagaacaa ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc
     5341 tgggcggttc aatgctgatg caggggctga ttagcgccga gcagctggca caggcgctgg
     5401 cagagcaaaa cggcgtggcg tgagaatcca tcgatgcctg gcagatcccg tcctcgctga
     5461 ttgccgaaat gccagcctcc gtagcgctgc attatgcagt actgccgctg cgtctggaaa
     5521 atgatgagtt aattgtcggc agtgaagatg gtattgaccc ggtttcgctg gcggccctga
     5581 cgcgtaaagt cggacgcaaa gtgcgttacg tcattgttct gcggggacaa attgtcacag
     5641 ggttacgcca ctggtatgca cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg
     5701 cggttcagca tcagtggctc acggaacagc agaccggtga aatctggcgg caatatgtgc
     5761 cgcatcagtt cctgttcgcc gaaatactga ccacgctcgg tcatattaat cgttcagcaa
     5821 ttaacgtgtt gttattgcgc catgaacgca gttctctgcc gctcggcaag tttttggtca
     5881 ccgaaggcgt tatcagccag gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac
     5941 aagtttcgat gcaatcacta ttactcaaag caggtttaaa cacagaacag gttgcgcaac
     6001 tggagtccga aaatgaagga gaataacctt aatcgcgtca tcggatggtc tggtttactg
     6061 ctgacgtctt tattgagtac cagcgcactc gcagacaata tcggcaccag cgcagaagag
     6121 ctggggctga gcgattatcg ccattttgtt atttatcccc gtctcgataa ggcgctgaag
     6181 gcacagaaaa ataacgacga agcaaccgcc atccgcgaat ttgaatatat acaccagcag
     6241 gtgccggata atattccgct gactttatac cttgcggaag cctatcgtca ttttggtcat
     6301 gatgaccggg cgcggctgtt gcttgaggat caactgaaac gtcacccagg agatgcccga
     6361 cttgagcgca gtctggcggc tattccagtt gaagtgaaaa gcgttacgac tgttgaagaa
     6421 ctgcttgccc agcaaaaagc gggcgacgct gcgccgaccc tgcgttgtcg cagtgaagtc
     6481 gggcagaatg ccctgcggct ggcacaatta cctgtcgcca gagcgcaact gaacgatgcg
     6541 acgtttgctg catcgccgga aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc
     6601 tacctgaaac aatggtccca ggcagatacg ctatacaatg aagcacgcca gcagaacaca
     6661 ttaagcgcgg cagaacgccg tcagtggttt gacgtgcttc ttgccgggca gctggacgat
     6721 cggatcctgg cactgcaatc acaggggatc ttcaccgatc ctcagtcata tattacttac
     6781 gcgaccgcgc tggcttatcg tggcgaaaaa gcacgcctcc agcattatct cattgaaaat
     6841 aagccgctgt ttaccacggg cgcacaagag aaaagttggc tctatctgtt atctaaatac
     6901 agcgccaacc ccgttcaggc attggcgaat tatacggtgc agtttgccga taaccgccag
     6961 tatgttgttg gcgcgacgct accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa
     7021 aaactgctcg ccaccctccc cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg
     7081 gcgacccgta acaaggctga atctctgcgt ctggcacgat tgctgtatca gcaagaaccg
     7141 acaaatcttc cccgcctgga tcaactgacc tggcaactga tgcagaacga gcagtcacgc
     7201 gaagctgccg atttattgct gcaacgctat cctttccagg gcgatgcgcg tgtcagccag
     7261 actttaatgg cgcgactggc gtctctgctg gaaagtcatc cttacctagc aacgccggcg
     7321 aaggtggcga ttttatcgaa acccttaccg ctggcggagc aacgtcagtg gcaaagtcag
     7381 ttgccgggta ttgcagataa ttgcccggca atagttcgct tgctgggcga tatgtcgcct
     7441 tcctacgatg ctgccgcctg gaaccgtctg gcaaagtgtt atcgggacac gctacccggt
     7501 gtggcgttgt atgcatggct tcaggccgaa caacgacaac cgagcgcctg gcaacatagt
     7561 gcggtagcct atcaggcgta tcaggttgag gactacgcca ccgcactggc ggcctggcag
     7621 aaaatcagtc ttcacgacat gagcaatgag gatctgctcg ccgccgccaa caccgctcag
     7681 gcagcgggaa atggcgcggc tcgcgatcgc tggcttcaac aggcagaaca acgtgggctg
     7741 ggaaacaatg ctcaatactg gtggctgcat gcgcaacgtt atatcccagc tcagccagaa
     7801 ctcgcactga acgatctcac gcgctcaatc aatattgccc cttctgccaa cgcttacgtt
     7861 gcgcgggcga caatttatcg ccaacgtcat aatgttccgg ccgcggtgag tgatttgcgc
     7921 gccgcgctgg aactggaacc gaataatagc aacacccagg cagcgcttgg ttacgccttg
     7981 tgggatagcg gtgatatcgc acagtcgcgg gaaatgctcg aacaggcgca taaagggctt
     8041 ccggacgatc cggcactgat ccgacaactg gcctacgtga accagcgtct ggatgacatg
     8101 cctgcgacgc agcactacgc ccggctggtg attgatgaca ttgataatca ggcgctgata
     8161 accccactga ccccagagca aaatcagcaa cgcttcaatt tccgctgttt gcatgaggag
     8221 gtcggtcgcc gctggacgtt cagtttcgat tcttccatcg gcttgcgttc cggcgcaatg
     8281 agtaccgcta acaataatgt cggcggtgca gcgcctggga aaagctatcg tagctacgga
     8341 caactggaag ccgagtaccg catcggacgc aatatgctgc tggaaggcga cctgctctca
     8401 gtttatagcc gcgtctttgc cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat
     8461 ccgatgtccg gcaccggtct gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc
     8521 gtcgaacagc agttgccgct gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc
     8581 agcgcctcat tctttaatgg cggcaaatac agcgacgaat ggcacccgaa cggttcaggc
     8641 tggtttgccc aaaacctgta cctcgatgcg gcgcaatata tccgccagga tattcaggcg
     8701 tggacggcag attatcgcgt cagctggcat cagaaggtag ctaacggaca gactattgag
     8761 ccttacgctc acgttcagga caacggctat cgtgataaag gcactcaggg cgcgcagctt
     8821 ggcggtgtcg gggtccgctg gaatatttgg accggcgaga cgcactacga cgcctagccg
     8881 cacaaagtca gtctcggcgt cgagtatcaa cataccttta aggcgattaa tcaacgtaac
     8941 ggagagcgca acaacgcgtt tctcaccatt ggagtacact ggtaaatgcg taagttcatt
     9001 ttcgtattgc tgacactgct tttggtcagc cctttttcct ttgcgatgaa aggtattatc
     9061 tggcaaccac aaaaccgaga tagtcaggtt accgataccc agtggcaggg gctgatgagt
     9121 cagttacgtt ttcaaggctt cgataccctg gttttgcaat ggacccgtta cggcgatgca
     9181 tttacccagc cagaacagcg cacgttattg tttaagcgtg ccgcagctgc gcaacagact
     9241 ggcctgaagc tgattgtcgg gctgaacgcc gatccggaat tttttatgca ccagaaacag
     9301 tcgtctgcag cgctggaaag ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc
     9361 agattatgga gcgccgcgcc tggcataacg ccggatggct ggtacatcag cgcggaaatt
     9421 gacgacctga actggcgcag cgaagccgcc cgtcaacctt tgctaacatg gttaaacaac
     9481 gcgcagcggc tgattagcga tgtttcagca aaaccggttt atatcagtag ttttttcgcc
     9541 ggaaacatgt cgcccgatgg ctatcgccaa ctgctggaac aagttaaagc aaccggagtt
     9601 aatgtctggg tacaggatgg cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt
     9661 tatctacagg ccagcgccga ttgccaaagc tccgccccag caagcggcat tgtttatgaa
     9721 ctgtttgtcg ccggcaaagg caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt
     9781 gcctcgctgt tagcgaaacg ttcctcttgc ggtaaagaca tgctctattt ctctctgcgc
     9841 tatttgcccg tcgcgcacgg cattctcgag tattaaatcc cctccaggta agtcgggtac
     9901 gacctggctt acctctttcg ctctcgtcag aattccatca agattaattg ctaaaaagca
     9961 tgctaaagaa agaagatcgt tatcggcgaa cgggccgatc actcatgcag gaagcataga
    10021 tcctttgagc ggagtttctg atatctgagg agtgcgaaat gcaattgagc agcagtgaac
    10081 cttgcgtggt gatcctgacc gaaaaagagg tagaggtaag cgtcaataac catgctacgt
    10141 ttacccttcc gaaaaactac ctggccgcct tcgcgtgcaa caataacgtc attgaactct
    10201 caacgttaaa tcacgtatta atcacccaca tcaaccgtaa catcatcaac gattatctgt
    10261 tgtttttaaa taagaaatta acctgtgtaa agccctggtc gcggctggca accccggtta
    10321 tcgcttgtca tagccgtaca ccggaagtgt tccggctagc cgccaaccac agcaagcagc
    10381 aacccagcaa accctgcgaa gcggagttga cgcgcgcatt actttttacc gtattgtcta
    10441 actttcttga gcaatcgcgg tttattgccc tactgatgta tatcttacgc agcagcgtcc
    10501 gcgacagcgt ctgccgcatt attcaaagcg atattcagca ttactggaat ctgcgaattg
    10561 tcgccagttc gctatgttta agccccaggc tgctcaaaaa gaaattaaaa aacgaaaata
    10621 ccagctatag ccagattgtc acagagtgtc gtatgcgtta cgccgtacag atgttattga
    10681 tggataacaa aaatatcact caggtggcgc aattatgtgg ctatagcagc acgtcgtact
    10741 ttatctctgt ttttaaggcg ttttacagcc tgacaccgtt gaattatctc gccaaacagc
    10801 gacaaaaagt gatgtggtga agggcaaagc ggaaacggat aagacgggca taaatgaaga
    10861 agaaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc
    10921 tatccaactg agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac
    10981 ggtcaaccct tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg
    11041 tctgattttc tgatttattt cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga
    11101 atcaagaaaa cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg
    11161 tccgctccta tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat
    11221 cctatcatca gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc
    11281 ctgaaaacgg atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta
    11341 atcattatgg atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa
    11401 caaatccaga gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct
    11461 ggcagagcga tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat
    11521 atttttcatg ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt
    11581 aactatataa aaagcaataa atgtagtacg aatagttcaa cggtcactgt gctatctaat
    11641 cgtgaagtga ccatattacg ttatctggtt agcaataaaa cagttagtgc gcataaatct
    11701 aatatttatg gc
//
LOCUS       DADKXM010000118        11628 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.119, whole genome shotgun sequence.
ACCESSION   DADKXM010000118 DADKXM010000000
VERSION     DADKXM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.119"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..427
                     /locus_tag="JQR05_003117"
     CDS             <1..427
                     /locus_tag="JQR05_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY5325207.1"
                     /translation="ITGPGGNDGLEGVSYIPYKDIIGVWTVCHGHTGKDIMPGKTYTE
                     AECKALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQG
                     DIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            424..894
                     /locus_tag="JQR05_003118"
     CDS             424..894
                     /locus_tag="JQR05_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY5325208.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRAADALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(983..1495)
                     /locus_tag="JQR05_003119"
     CDS             complement(983..1495)
                     /locus_tag="JQR05_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5325209.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1690..2454
                     /locus_tag="JQR05_003120"
     CDS             1690..2454
                     /locus_tag="JQR05_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325210.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGKKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2405..3808
                     /locus_tag="JQR05_003121"
     CDS             2405..3808
                     /locus_tag="JQR05_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY5325211.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3808..5274
                     /locus_tag="JQR05_003122"
                     /pseudo
     CDS             3808..5274
                     /locus_tag="JQR05_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5165..5908
                     /locus_tag="JQR05_003123"
     CDS             5165..5908
                     /locus_tag="JQR05_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY5325212.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQP
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5912..7132
                     /locus_tag="JQR05_003124"
     CDS             5912..7132
                     /locus_tag="JQR05_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY5325213.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7136..7510
                     /locus_tag="JQR05_003125"
     CDS             7136..7510
                     /locus_tag="JQR05_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325214.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVIH"
     gene            7494..7643
                     /locus_tag="JQR05_003126"
     CDS             7494..7643
                     /locus_tag="JQR05_003126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325215.1"
                     /translation="MSSSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADGNAE
                     ISWKI"
     gene            7655..8596
                     /locus_tag="JQR05_003127"
     CDS             7655..8596
                     /locus_tag="JQR05_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5325216.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DISIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNQLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSIPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTHLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(8593..8826)
                     /locus_tag="JQR05_003128"
     CDS             complement(8593..8826)
                     /locus_tag="JQR05_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325217.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            8825..9232
                     /locus_tag="JQR05_003129"
     CDS             8825..9232
                     /locus_tag="JQR05_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY5325218.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLTE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            9229..9783
                     /locus_tag="JQR05_003130"
     CDS             9229..9783
                     /locus_tag="JQR05_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325219.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAESA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRAAGESPGTELA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            9770..10159
                     /locus_tag="JQR05_003131"
     CDS             9770..10159
                     /locus_tag="JQR05_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HAY5325220.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            10134..10697
                     /locus_tag="JQR05_003132"
     CDS             10134..10697
                     /locus_tag="JQR05_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325221.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPAETSWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGTAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            10701..>11628
                     /locus_tag="JQR05_003133"
     CDS             10701..>11628
                     /locus_tag="JQR05_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY5325222.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSHASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQSEAGITRLLSSVEKSLDQAV"
BASE COUNT         2988 a         2807 c         3214 g         2619 t
ORIGIN      
        1 aatcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
       61 tatcattggt gtatggactg tatgtcacgg acacaccgga aaagacatca tgcctggtaa
      121 aacgtatacc gaagcagaat gcaaagctct cctgaataaa gaccttgcca ctgtcgccag
      181 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      241 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg acgcttcttc gcaaaataaa
      301 ccagggcgat atcaaaggcg catgtgacca gctacgtcgc tggacatatg ctggcggtaa
      361 gcaatggaaa gggctgatga cccgtcgtga gattgagcgt gaagtctgtt tgtgggggca
      421 gcaatgagca gagtcaccgc gattatctcc gctctggtta tctgcatcat cgtctgcctg
      481 tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagagca gcgcgacaaa
      541 aacgcaagag aactgaagct ggcgaacgca accattactg acatgcagca gcgccagcgt
      601 gctgctgatg cactcgatgc taaatacacg aaggagttag ccaatgcgaa agctgaaaat
      661 gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg aaaatgccct
      721 gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc caccgtcgaa
      781 ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat cagcgaccag
      841 acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg gtagcggtaa
      901 ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga agctggattc
      961 gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta aaactaccaa
     1021 cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact ttcactggat
     1081 gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg ataaagcgcg
     1141 aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt tctattgtat
     1201 tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt ataccaagat
     1261 ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca gcttcccatg
     1321 gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat gctggtggca
     1381 atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga agcccccata
     1441 ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc atcaatgtct
     1501 ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt aggcttaaac
     1561 accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg caggagtgag
     1621 tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta aatcacagga
     1681 agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg ggccggagtc
     1741 cttagcctcc gtgatatagg caagaaatac ggcgttacag aaggggctat caggaagagg
     1801 gccaaaaagc ttggttgggc acgcagtggc ggtacgcagg tttgcaaaaa tggtacgcaa
     1861 aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca aaaaagtacg
     1921 caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg taccgatccc
     1981 ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg ttatgcccgt
     2041 cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct cgaggacgaa
     2101 ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg gtggttggtg
     2161 tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa tatcagcgcc
     2221 gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg cacgcttgcg
     2281 acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga taaggtgtct
     2341 ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg agagcgtgac
     2401 ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac gtagtttctg
     2461 gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa aatcatggga
     2521 tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt gttgtgcccg
     2581 tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc agatagacag
     2641 gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc gggttactgg
     2701 ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt cactggaggg
     2761 gatcgatgtg ttgtggctgg aagaagccca cgcactgacg gaataccagt ggaaaattct
     2821 ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc ccggacttgt
     2881 tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc tcatccgcaa
     2941 aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta tcgacgcggc
     3001 gcgacgccgt gatccggatg gttttaaaca tgtgtatgag ggcgttccgg agtctgatga
     3061 tgatgcggca atcatcaaac tgtcttggat agaagccgca gtggatgcgc acaaaacgtt
     3121 aaatttcgaa cccagtggaa gaaagcgtat tggctttgac gtggctgaca gtggtacaga
     3181 taagtgcgct aacgtttacc gtcacggatc cgttgttttc tgggccgacg aatggaaggc
     3241 caaagaagat gaattactga agagctgcca gcgtacttat caggcggcgc tggagcgtga
     3301 agcagatatt gtttacgact ctatcggtgt tggtgcgtct gccggtgcta aattctctga
     3361 aattaacgct gaccggaaga gcgagaacgc atacgcgcga cgtgtgaatt accagaggtt
     3421 taacgccggt gctggtgtgc atgagccaga tgacgagtac aacggcatcc ccaacaaaga
     3481 ctttttcgca aatcttaagg ctcaggcatg gtggctggtg gctgaccgtt tcagaaatac
     3541 gtttaacgcc attaacaacg gagaacagta tcctgtggat gagctgatca gcatagattc
     3601 tcgttgtccg ttgcttgaaa agctgaaact ggaactgaca acacctcatc gtgatttcga
     3661 ccgtaacgga cgtgtgatgg tcgaaagtaa aaaagacctc gcaaaacgcg agataccgtc
     3721 accaaacgtt gctgatgcat tcattatggc cttcgcgcca atcgatacat cgctggatat
     3781 ctgggaacag ctggggagac aggcctgatg gcacgaaaca aacaagccct gcggcgaact
     3841 gtgcaggcca cagctgatgg ttatgagaat tttattgccc gcgtagggat gcagacacct
     3901 aaccagcact cagcatccac ctaccgggct aatttcacta gtcgtaaccg catgctggtg
     3961 gaatggtcct atcgttcatc ctggatcatc ggcgaagcag tcgatgctat cccggatgat
     4021 atgacccgca aaggcattcg catcacttcg gaaattgatg caaaagatcg tggcattctc
     4081 gaatcacaac tggatgagtt gcaaatctgg gatgcgctga atgacgtgct gaaatggtcg
     4141 cgcctctacg gcggcgcggt gggtttcatc atgattgagg ggcaggcacc aatgaccccg
     4201 ctgcgacccg aaaccatcgg taagggcaag tttaagggga ttctcccgct cgaccgctgg
     4261 atggtcgacc cggcactgac ccgccgcatt aaagatatgg ggccggacct gggtaaacct
     4321 gagttttacg atgtggtgac cacagcaacg ggaattcctg cctagcgcat tcatcacagt
     4381 cgcctgattc gctttgatgg cgtcacgttg ccatttcagc agaagatgac cgagaacgaa
     4441 tggggaatgt cggttgtaga gcgtatctgg gatcgtctta ccgcgttcga cagcgctact
     4501 gtcggcgcgg cgcagctggt ctacaaggcg catttgcgta cctacagcgt ggagaagcta
     4561 cgcgagctta tcgcacttgg tggtcctgcg tatgaagcgt tgctgaagaa tatcgacctg
     4621 attcgacagt tccagagtaa tgaaggcatg acgctcatgg actcgcggga taagtttgaa
     4681 acccatcagt acagcttcag tggtctggat gacatccttt cgcagtttgc agaacagatt
     4741 agtggcgctg ttggtatccc actggtgcgg ttgttcggac agtccccgaa aggattttct
     4801 accggtgatg cagaccttgc caactattac gatcgcatca gttcgttgca ggagaggcgt
     4861 ttacgtcttc cggtgcggcg gatactggac atcatgcatc gttcggaact tggcaagccg
     4921 ctgccggacg atttcacgtt tgagtttaac ccgctctggc aaatgtctga tgtcgatcgc
     4981 tcaacggtgg cgttaaacac caccaacgca atcagtacgg cgctgggtga tggtctgatg
     5041 acactgaaag ccgctatgac tgatttgcga gaaaattctg acgtaaccgg catcggggca
     5101 tccattaccg acgaggacat cgagaatgcc gaagatgaag cgccgcccgg catcggcgaa
     5161 cctgatgacg aaccgcagga gccgtcaggc ggaaatccgg tatcgaacca gcctacgcag
     5221 gatagcgagg gcggtcggag acatcgtaaa tggtcgctac gatggttcaa atgacagtat
     5281 cacggaaatt attgcggcgc tggagcgtta cagtgaaatc atcaccccct gggcgacaaa
     5341 ggtcgcggaa aactttactg cggacctaac ccggcagaac gagaaagttt ggcggcaaca
     5401 cagcaagaac atcagtcgcg agctccgcaa tcttgtggaa agcgctcctg tgggccaggt
     5461 gatgcaaccc atcatcgccg aacaggtcaa gtacatcaaa tctctgcctc tcgaggccgc
     5521 agacagggtg tacgacatcc agaacaaagc gatagaggct gttgtcaccg gtggtcgggc
     5581 ggagcagttt gctaaagaga ttgcatctac cggcgatgtt gctaagtcta gggccgatct
     5641 gattgcccga acggaactgg gaagagcaac gggcgcgctg gatatggccc gagcgatagc
     5701 tattggctcg gatggttata tttggcgtac agccgatgat ggcgatgtcc gagattccca
     5761 cgatcacatg aaaggtaaat tcgtccgctg ggattcacct ccaactttgg acggcatgac
     5821 cggccacgcg ggcgagctgc caaactgccg ctgctataaa gagatcgtgt ttgttcgcgt
     5881 tccattcgca atgaaaaggg cagcataacc catgaaatac ttttttgaga ccaggctcgg
     5941 ggaaacccga taccgcctgg ctgacggctc gttgctgtgc aaagacgtgc cgataggacg
     6001 aacaggtaag cagctctatg gtgctgatga cctgccaaaa ctgaaacccg ataagttcgg
     6061 tgaaatagtc gtcacgcgtt ctcctgagca ggtattccat ccggccacgc ttgcctcatt
     6121 cgaagggatg agcatcacga tcctgcatcc tgaagatgaa aacgggaatg tgcggctggt
     6181 aaatcccgag aactggaaag agcttgctgt cgggcacctc cagaatgtcc ggcgcgggac
     6241 gggtgagcag tctgatttga tgctggctga ccttatcgtc aaagacgaaa gcgccattca
     6301 gcttatcgaa gatggcctgc gcgaagtgtc gtgcggctat gacgcggagt acgagcagac
     6361 cgagccaggt aaagccgagc aggtcgatat taccggaaac catgtggctc ttgtccctaa
     6421 aggcagagcc ggaaatcgtt gtgcaattgg agacagagac acaatggcaa atcaaaagaa
     6481 aaactggtgg aaccgcatgc gtgcggccat caagacagga gatgccgaca ccatgaacga
     6541 actggtggag tcggctcccg catcggttac aggagatgag ggggatttgc cgcagggcgt
     6601 taatctcaac atcaacctgt ccccgcagca accactaccg gacaaagcac cagagatggg
     6661 cggagatcca accggcgaca gtgatgatga cctcaaaaca ttactgaaag ccctgctggc
     6721 taagctggaa agaaatgcca cgggcgataa cgataataag cctgacgata atccgaccgg
     6781 tgacggcgag gacgatgaag aggaaaccac gattactggt gactcagcct ggcgtgccga
     6841 agttatcgtt ccgggtatcg atctgagccg taagatgaaa ccgaccgcgt tcaaacgcga
     6901 ggttctggct tctgctgaca aaacgctggt tcgccagata gtcggtgatg cggatatccg
     6961 caaattaccg aaacaatcgg tcgacatggc gtttaatgcc gtgtctgagg ttgccaaagg
     7021 gcgaaacacc cgcgccacca ccggcgatgc acagcgccta aacatgggca tgaccagtat
     7081 cgcttccctg aacaaacaaa acgctgaatt ctgggcaaac cgtaaagggt aaaaaatgaa
     7141 taatgtattt ctgtaccgga tgcctgttgg cattgccggg gctgtctctc gcccgcagga
     7201 cttaaccgtc gaaccggtgg tccttaaatc cgataacgcc ttcgctgcct atggcctggc
     7261 tggtaaatac gatgatgacg gttttttcgt gccgctggca gatggtgata ccgcagacaa
     7321 ggtgaagggg atctacgtgc gcccttatcc gaccacgtcg cagccggaca tggttcgcca
     7381 ggtgggaaca ggcaagaact tcccgggcga cgccatgaag cgtggctacg tgaccgttaa
     7441 tctcggttct gattttgatg ccagcactat caaaaaaggc gatccggtat acgttgtcgt
     7501 catccactga tgaatccatc aaagtgccgc tgggtggatt catgtccacg tcagtcagtg
     7561 gcaaaaacgt ggtgctgacc aacgctgaat tcacaggtgc cggtgatgct gacggcaatg
     7621 cagaaatttc ctggaagatt taaggaacag acgaatgatt acttttgatc aggcaaccgt
     7681 tgacagctct ggtgcctttc tcatcgggga gctggagcga ctcgaccaga cgctgaacct
     7741 gccactggtg gggtacacct ggagccgcga tattcaactg cgtgaagaca tctccatcgc
     7801 agatgacatt tccagctgga cgaataccag cttcgccgct gcgggtactg gcgcaaatcc
     7861 gaatggaaaa aactgggtag gcaaagactc aaccgctatt gctggcgtga acgtggatat
     7921 cggcaaatcc ggtaaccagc tgaacctgtg ggggatggaa cttggctgga cggtcataga
     7981 attgcaggct gctcagcagg tcggccgccc gattgatacg cagaagtatg acggtatgca
     8041 actgaaatgg cagatggata acgatgaaca ggtatatgtt ggcgattccg cattaaacct
     8101 gaaaggcctt gttaccctgg acggcgtgcc tgtcaacaac gctgccaaaa cgtgggcaac
     8161 ctcaataccg gacgaaatcc gcgcaagcat taaccaggtg ctgtctgatg cgtgggccgc
     8221 ttctggttac tctgtggttc cgcgtgattt gctgatcccg cctgagcagt ttgctctgtt
     8281 gtccagcatc atcgtttcat ctgcaggtaa ccagtccctg ttgacgcacc ttcagaccaa
     8341 caccatcagc tatcaccaga acggtgttcc gctgaatatc cgcgcggtta aatggctgaa
     8401 aggccgtggt gtggggaata aggatcgcat ggttgcgtac accaacgata aaaaatacgt
     8461 ccgctacccg ctggttccgc ttcagagcgt gccggtgcag tatcgcggtc tgtatcagat
     8521 cgtcacttac tacggcaagc tgggtgcagt cgagccagtg tacaaagaaa ccatttcgta
     8581 cgttgatggc atttaacagc cacatggccc cctggcgggg ccattaagga tgacccgatg
     8641 gcaaaaaatg atgcagtaat acacgtacat accccgtttg tgctcacgct tcccgacggt
     8701 tctcggcgcg agtttgttaa aggccgtcat gttgtggagg aagacgttgc cacgcactgg
     8761 ttcactcgtg cgcacgcgga agtatccgtt ggcaaagcca cagacgcgcg taacgaggta
     8821 aaaaatgcca aagaatcaaa gtctgccagc ggtaagtgat tttcgccgcg acttcccgca
     8881 gtttgctgac cctgccaaat atcccgaagc gcaaatccag tttcgtctga atctggccga
     8941 tgaactgctg accgaaaacg tcaccggcaa aaagttgttt ccgtactttg ccggattgtt
     9001 cgttgcgcac tacatgacgc tctgggcggc agacagcaga gcgatgctgg ctggtggtcc
     9061 gggcggttca accaatggtg ttcagtcctc aaagtccgtt gacaaggtaa gcgtcagcta
     9121 tgacaccagc gcgacgctga atcctgatgc aggtttctgg aataacaccc gatatggcgc
     9181 tgaattttat cagttgatca cgatgttcgg tgcaggcggt cgccagctat gagtttcaaa
     9241 agcggtgtaa caacgagggt ggataacgct aaggccattc tggatgcgct caggtcgtta
     9301 accaaaaaag atgtgctggt cggcatccct tcggaagaca gcgagcggga tgatgttccg
     9361 tttggtaatg cgggcatcgg ttacctcaac gaatacggct caccagagca gaacatcccg
     9421 ccacgacctc acctggttcc cggcgttaaa tcggcagaag agcagacggt gccgcagctc
     9481 aaagccgcgg cgcaggctgc acttgatggt aatgctgcgg gagcagaaag cgcactcaac
     9541 cgtgccggaa cactggccgc taatggcgtc aggcgttaca tgaccattac cggctttacg
     9601 ccgcttgctg acagtactgt tgaagcccgg gctcgtcggg ggcgcaaggg ggcaacactg
     9661 gaacttgccc ggcgtgctgc tggcgaatct ccgggaaccg aactggcgaa accattaatt
     9721 gacaccgggc aatatcgcag agcgattacc catgttgtga gggataaaga tgccgactct
     9781 tgatgtaaca gatgtgcttt ttgaccccga tttttgcgac ttcaatttgt gggtaacacg
     9841 ccgtgtgcaa acggtggatg aggatgggat cggcagcgac agcgaagtta aaaagcagtt
     9901 tgccggagtc gttactgttg atcgctctct ggaaaaccgt cgtatgcagg ccgggcaggt
     9961 aatcagtggt gcaattctga ttgtgacgac tgagcgactg acgcagggac agactggccg
    10021 tgatgccgat atcgtgacgt atcagggccg tgattatcgt gtgactttcg tcgacccgta
    10081 tacagcgtat ggtgccggat tcgttcaggc gcattgtgag ttgctgccgt ttgatggggg
    10141 aattccggtt gagcaataac accagcacag agcgcggatg gctgatacca accagtggcg
    10201 atccggatta tgacgaagcg ctcgacaggc tgttaagcca gtggatgcgt aacgtttccg
    10261 gtctgtctgc cgggatggtt cgtccgcgct ggcagaaaga gcagccgcca ctgctaccgg
    10321 ctgaaacgag ctggtgtgcg tttggggtta tcggatggtc aggtgatgac agtccggcat
    10381 tcaccagaca gaccgatgat ggctctcagc tctggcggca tgaaacgatt gagtgtatgg
    10441 cttcgtttta tggtacggcg gggatggtgt atgcgtcccg gtttcgtgac ggtatatctg
    10501 taccgcagaa caatgcagca ctgaatgcgc tggggctgtc tcttggcgat tacacaggtc
    10561 tgactccctt ccctgaactt attaatcagc aatgggtccg ccgctacgat atgacggtgc
    10621 gtctgcgccg gaaggttgtg cgcgagtacg gtattaaatc gctggtggaa gcaccagtca
    10681 tctttttcgg agattaagct atggcacagg gcttgcctgt atcaaacgtt gttaatgttg
    10741 atgtgatcat gtcgccgcgt gcagcatcag ggcgaaattt tggtgcatta ctcattctcg
    10801 gcccgtccac aatcattccg gtaagtgagc gcattcgccg ttattctgct gcggaagata
    10861 ttggaaaaga ttttggcgtg gaatcaccag aatataaggc tgcgcaggtg tttttctcac
    10921 aatcaccgaa acctcaggag gtttttgttg gtcgttgggt gaaaacgaag ggagacagcg
    10981 aacaggccac gcctgagacg ctggagcagg ctgtgaatgc catgcttgat tatacttcat
    11041 ggtatgggct ggggattgca gacgatgcag atattccgga tgcagactgg ctgaaagtgg
    11101 ctgcggcgat cgaatcctct tctgtaagcc gtattctggc gattacgaca agcgatgaga
    11161 aatgcctgca gactgcatcc agcgatgatt tggcatcaaa actgaaaacc gccggatatt
    11221 cacgcagttt tattcagtat tcatcgggta ataaatacgc tgcgttatct gcatttggcc
    11281 gggcattcac ggttaatttc aatggcagta ataccgcgat tacgctcaag tttaagcagg
    11341 agccgggtgt cgggtatgaa acactgacag tcagccatgc atcggcactt gatgcaaaaa
    11401 actgcaatgt attcgtgtac taccagaatg atacggctat cctccagcag ggagtgatgg
    11461 ctaacggcga tttctttgat gaacgccacg gcctggactg gttacagaat tatgtgcaga
    11521 ccaacctcta taacctgctt tataccagca ccacgaaagt accccagagt gaagccggta
    11581 ttacccgact gttatcaagt gttgaaaaat cactggatca ggccgttc
//
LOCUS       DADKXM010000119        11601 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.120, whole genome shotgun sequence.
ACCESSION   DADKXM010000119 DADKXM010000000
VERSION     DADKXM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.120"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..430
                     /locus_tag="JQR05_003134"
     CDS             <1..430
                     /locus_tag="JQR05_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY5325223.1"
                     /translation="NVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPL
                     SDLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQV
                     TKDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(462..1298)
                     /locus_tag="JQR05_003135"
     CDS             complement(462..1298)
                     /locus_tag="JQR05_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY5325224.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            1556..2224
                     /gene="folE"
                     /locus_tag="JQR05_003136"
     CDS             1556..2224
                     /gene="folE"
                     /locus_tag="JQR05_003136"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY5325225.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            2241..3398
                     /locus_tag="JQR05_003137"
     CDS             2241..3398
                     /locus_tag="JQR05_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY5325226.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            3540..4580
                     /gene="galS"
                     /locus_tag="JQR05_003138"
     CDS             3540..4580
                     /gene="galS"
                     /locus_tag="JQR05_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY5325227.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AVAITNSTNQAM"
     gene            4860..5858
                     /gene="mglB"
                     /locus_tag="JQR05_003139"
                     /pseudo
     CDS             4860..5858
                     /gene="mglB"
                     /locus_tag="JQR05_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
     gene            5919..7439
                     /gene="mglA"
                     /locus_tag="JQR05_003140"
     CDS             5919..7439
                     /gene="mglA"
                     /locus_tag="JQR05_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY5325228.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNTKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            7455..8465
                     /gene="mglC"
                     /locus_tag="JQR05_003141"
     CDS             7455..8465
                     /gene="mglC"
                     /locus_tag="JQR05_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY5325229.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDTTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLVAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8536..9771)
                     /gene="preA"
                     /locus_tag="JQR05_003142"
     CDS             complement(8536..9771)
                     /gene="preA"
                     /locus_tag="JQR05_003142"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY5325230.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(9765..11003)
                     /locus_tag="JQR05_003143"
     CDS             complement(9765..11003)
                     /locus_tag="JQR05_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY5325231.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(11197..11436)
                     /locus_tag="JQR05_003144"
     CDS             complement(11197..11436)
                     /locus_tag="JQR05_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY5325232.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(11439..>11601)
                     /locus_tag="JQR05_003145"
     CDS             complement(11439..>11601)
                     /locus_tag="JQR05_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="involved in vancomycin sensitivity and other
                     barrier functions of the cell envelope; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY5325233.1"
                     /translation="FGALADLYIFKREPRFLGPLVPIPAMHQVPEDAQGYPAVTPEQL
                     LELQKKQGK"
BASE COUNT         2860 a         2845 c         2964 g         2932 t
ORIGIN      
        1 caacgttaac aaagctcgta ttcagggcgt ggaaaccgaa ctgaaaattc cgttcaacga
       61 tgaatggaaa ctgtcgatca actacaccta caacgatggt cgtgatgtca gcaacggcga
      121 aaacaaaccg ctatccgatc tgccgttcca tactgctaac ggtacgctgg actggaaacc
      181 gctggcgctg gaagactggt cattctatgt ttctggtcac tataccgggc agaaacgcgc
      241 cgacagcgcg acggctaaaa caccgggcgg ttataccatc tggaataccg gcgcggcctg
      301 gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg aaccttggcg acaaggatct
      361 cagtcgtgac gactacagct ataacgaaga cggacgtcgt tactttatgg cagtggatta
      421 tcgcttctga tgagaagatg cccggcgaac cgggcggact ttcacttcag taaatactgt
      481 gcatggaagc gcaggtgatc ctctataaaa gaggcgatga agtagtaact gtgatcatat
      541 cccggctgaa tacgcagcgt catcggccac gctttctggc gcgcggcttc tgccaatacc
      601 gcaggttgca actggtcggc aagaaactga tcattatcgc cctgatcgat aagcgtcggg
      661 atcgcatcct gcgcgttact ggcatacatc agcgcgcaac tatcccattc cagccatgca
      721 tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac gctgcacgga
      781 ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt cagcgccata
      841 atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc gctgacatta
      901 aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat ccggtaatgc
      961 gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc taaatcgtag
     1021 ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac cagtacaatc
     1081 cccagttccg ccgctacccg ctgggcacca gccttggtgg tgaagttctc gtcattgcag
     1141 gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg gggtgggaga
     1201 aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtctcca gcgttgctgc
     1261 cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt gttgtgttgt
     1321 ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc acttccgtat
     1381 ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca ataattatcg
     1441 ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt aaatcagctt
     1501 aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat cataaatgcc
     1561 atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag gactggaaac
     1621 accactgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc ttattgctgg
     1681 tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca gtttgatgga
     1741 aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc tggattacgc
     1801 caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa tggtcaccgt
     1861 gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg atggcaaagc
     1921 gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta accgtatcgt
     1981 gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa ttcttattgc
     2041 gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg tgcattactg
     2101 cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct ctcttggtgg
     2161 attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc gccatcacaa
     2221 ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt cgcggcgtcg
     2281 ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag gcggcttatc
     2341 tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg gcatttctcg
     2401 atctcatcgg gcaggtgaaa ttcctcacgc tttttgcgct gctgtttggt gctggcctgc
     2461 aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg ttagtcttgt
     2521 tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct tacgggctgg
     2581 tgggcttaat ctgctggcgg ctggtgcgcg atgcgccatc ggtaaaaagc ctgtttaata
     2641 ccggcgtcat gctttatctg gtggggcttg gcgttttgct gttattgggg ctgatttccg
     2701 acagccagac cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact
     2761 ggaagcttca cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt
     2821 tactggcact gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg
     2881 cgctgatgcg cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg
     2941 gttttgtgct ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc
     3001 agctggactg ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg
     3061 cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc
     3121 gctttaagct ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat
     3181 tgcaaacgct gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc
     3241 gcctggagct gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta
     3301 tctggctgcg ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc
     3361 gtgctgccgg accggcaata tctaaaacat caagataacg atctggatca cattcgttaa
     3421 caaaacggct gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg
     3481 ctgacaaaat agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata
     3541 tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg
     3601 tgctcaataa cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga
     3661 gtgagctgga ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca
     3721 ccattggcgt ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg
     3781 tggatctggt cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag
     3841 cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg
     3901 ttcactcaaa agcattgagt gacgatgaac tggcacaatt tatggataac attcccggta
     3961 tggtgttaat caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc
     4021 tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt
     4081 atctttcttc cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg
     4141 cgttgaaaga gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca
     4201 tgccgggcgg tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg
     4261 tatttgctta taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca
     4321 ttgcgattcc gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca
     4381 ccgacccgca attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg
     4441 aactggcctt gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc
     4501 cgacgttagt gcgtcgccat tctgtcgcaa cgcgccagaa tgcggtggcg atcactaact
     4561 caacaaatca ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcctaacaa
     4621 tgtgatagct atgattgcac cgttttaacg ttgtaacccg tatgtaacag tgaataatca
     4681 ctttcaccga ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg
     4741 gcatggaaga acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct
     4801 actgccccgc cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca
     4861 tgaataagaa ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg
     4921 cacacgctgc tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt
     4981 ctgtagtgcg caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga
     5041 tgaatgattc tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga
     5101 aaggggtgaa ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg
     5161 agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg
     5221 cgctggatag ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta
     5281 ttcagggcga tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag
     5341 acggtcagat tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac
     5401 gtaccactta cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt
     5461 tagataccgc aatgtaggac accgctcagg cgaaagataa gatggacgcc tggctgtctg
     5521 gcccgaacgc caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg
     5581 cggtagaagc actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc
     5641 tgccagaagc gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg
     5701 ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg
     5761 cggctgatgg caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg
     5821 tagataaaga caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt
     5881 actgcgccct tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt
     5941 cttccgggga atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg
     6001 cacttgataa cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa
     6061 acggtgcagg aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg
     6121 gcaccatttt attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa
     6181 atggtatttc gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata
     6241 acatgtggct ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc
     6301 gcgaaaccaa agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg
     6361 gcacattatc cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataaca
     6421 cgaaaattgt gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc
     6481 tgttcactat tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata
     6541 aaatggaaga aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga
     6601 tcgccaccga accgctggca ggactgacga tggacaagat catcgccatg atggttgggc
     6661 gttctcttaa tcagcgtttc cctgacaaag aaaacaagcc gggagaagtc atcctcgagg
     6721 tgcgtaacct aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata
     6781 aaggggagat cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga
     6841 cgttatttgg tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca
     6901 ataaccataa tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc
     6961 gctcaacggg aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc
     7021 gcaactacaa aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt
     7081 gggtgattga ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct
     7141 ccggtggtaa tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat
     7201 taatgctcga tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt
     7261 taattgccga actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg
     7321 agttgttagg gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg
     7381 tcgatacaaa aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag
     7441 attaggggct tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg
     7501 gcggtattta tgtcgttctt ttagttttgc tggcgattat tattttccag gacacaacat
     7561 ttttaagtct gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg
     7621 cgctcggtgt ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg
     7681 tagggctggt ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag
     7741 tgttcccgga aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg
     7801 gtgcggtgat cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca
     7861 ttaccacgct cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg
     7921 tcggggcgtc gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg
     7981 tcgcgctggg gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt
     8041 tcgtctgggt gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta
     8101 acccggaagc ggcaaaagta tctggtgtca acgtcggcct gaacctgctg atgatctacg
     8161 cgttgtctgg cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg
     8221 ccaccaacaa cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg
     8281 gtgtatcgtt cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt
     8341 ttaccgtcat caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca
     8401 tcaaaggggc gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga
     8461 agtgatgtga ttgcggatat tgccggatgc gctgcatccg gcaatcggtg ccaaatgccg
     8521 gatgcgtctc tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac
     8581 ttccccgagc tcaatacaac ccaccgggca gacgtgacca caaagcagac aacccacaca
     8641 tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc
     8701 gccgtcgtaa caggaaatat aacagcgtcc acagccaaca catttgtcaa gattgatacg
     8761 gggatagaca atataactgc ggtctaaatc ttccgccggg acaatattgt tattcgccag
     8821 acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg agataatgcg ataacccgct
     8881 cgccatatct tctacgatgc gatagccgta ctgcataatg ccggtggtga cctgtaacgt
     8941 tgctgcgccg agcaataaaa actccgccgc atcttcccag gtttcaatgc cgccgatacc
     9001 gctgattggg aaatcgcgca gttctggatg ggtgcgcatt tgctgaatga agcgcagggc
     9061 gatcggtttt accgctttac cggaatatcc ggaaatactc gatttaccgt taacgatcgg
     9121 cataccgatt ttctgattaa gatcgatatt ggtgatggat ttaacggtgt taattgccgc
     9181 aatgccatct gcgccgccgc gcttcgccgc cagcgccact tcgcacatat caccgatatt
     9241 cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt ttcactgccc gacaatattt
     9301 ttctaccagc tccgggcttt gcccgacatc gctacccatc gcatgagaag tcatttgcgg
     9361 acaggagaag ttacactcga tcatatccgc gccagcttct tgcaccaggc gcgccagctc
     9421 ctcccattgc tgctcatttt cccccatgat cgaagcgatc aatactttgt ccgggtaatc
     9481 ttccttcagc cgacgcaggg cggtcagatt ctcttccaac ggatgttcag caatctgctc
     9541 catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa aacgcggcga
     9601 gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acaccgcccc aacctgtgtc
     9661 gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga ggcagaacgg
     9721 gttgggaaag ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct
     9781 ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac ggcatagacc
     9841 acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcggatc gcgggtctga
     9901 taattttgtg ttttgatggt gttacgctgc tgctctaact ccgcaaaggc atccagtctg
     9961 gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc gcctgataac
    10021 cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa tccatcgatg
    10081 atcgaaacgc ccagctcccg ggcgctggca aactcttttt cgcttgccgg aaactcatct
    10141 aactcttcac gtgctacgca agttaccgcc tgacagccga gaactttcag cgtgctggct
    10201 acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat gctgatatcg
    10261 ccttgcgcct gtcgtacacg ttgcaggaag tcgacggcaa tctcaacgtc gctatgctca
    10321 aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc gcggttttcc
    10381 gctttcagtt gctccagggt gagtgtttta ccgatttcgt tattgcactt aatggtcacg
    10441 cccatttttt caatacgggc gatctctgca tccagcactg attgcggtaa ccggaattgc
    10501 ggaataccgt tacgcaacca gccaccgggg tgcgcttctt tctcataaat cgtgacgtca
    10561 taaccctggt ttgtcagtgt cacactggcc tgcaatccgg caggacctgc gccaataatc
    10621 gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt ttgttgttca
    10681 aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac accggcacgg
    10741 gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac ggcaccgagg
    10801 gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta gattgagcga
    10861 ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc gtcgtgacat
    10921 aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg agtgagttca
    10981 tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat tatggcgaac
    11041 gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt taaactgtga
    11101 aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg agtgaagtaa
    11161 taattattct caataattaa cagaaaagag aaaatattaa cgatagaaaa taagataaac
    11221 aatcatgccg atagataaaa ttccgaagcc aatatatgcc accatataag cgaagaagag
    11281 tccgggattt tttgctcgcc agacatacac cggttccggt gcatttttaa cccgtttatc
    11341 aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg ggataaggaa
    11401 aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg tttcttttgt
    11461 aattcaagta actgttcggg tgtaacggcg ggatacccct gcgcatcttc cggcacctgg
    11521 tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt aaaaatataa
    11581 aggtcagcca gcgcaccgaa a
//
LOCUS       DADKXM010000120        11516 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.121, whole genome shotgun sequence.
ACCESSION   DADKXM010000120 DADKXM010000000
VERSION     DADKXM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.121"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(127..894)
                     /gene="hdhA"
                     /locus_tag="JQR05_003146"
     CDS             complement(127..894)
                     /gene="hdhA"
                     /locus_tag="JQR05_003146"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY5325234.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(1006..2034)
                     /locus_tag="JQR05_003147"
     CDS             complement(1006..2034)
                     /locus_tag="JQR05_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY5325235.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPATLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            2209..3801
                     /locus_tag="JQR05_003148"
     CDS             2209..3801
                     /locus_tag="JQR05_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY5325236.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPAALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3811..4983
                     /gene="malY"
                     /locus_tag="JQR05_003149"
     CDS             3811..4983
                     /gene="malY"
                     /locus_tag="JQR05_003149"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY5325237.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            5087..6088
                     /gene="add"
                     /locus_tag="JQR05_003150"
     CDS             5087..6088
                     /gene="add"
                     /locus_tag="JQR05_003150"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY5325238.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6124..7128)
                     /locus_tag="JQR05_003151"
     CDS             complement(6124..7128)
                     /locus_tag="JQR05_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5325239.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEDWGYDMRDGVLTRVEGEERV
                     EETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGIESAKHRATLCLA
                     "
     gene            7371..7496
                     /gene="blr"
                     /locus_tag="JQR05_003152"
     CDS             7371..7496
                     /gene="blr"
                     /locus_tag="JQR05_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY5325240.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7769..7984
                     /gene="ydgT"
                     /locus_tag="JQR05_003153"
     CDS             7769..7984
                     /gene="ydgT"
                     /locus_tag="JQR05_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY5325241.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            8070..8510
                     /locus_tag="JQR05_003154"
     CDS             8070..8510
                     /locus_tag="JQR05_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY5325242.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8587..9168
                     /gene="rsxA"
                     /locus_tag="JQR05_003155"
     CDS             8587..9168
                     /gene="rsxA"
                     /locus_tag="JQR05_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY5325243.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            9168..9746
                     /gene="rsxB"
                     /locus_tag="JQR05_003156"
     CDS             9168..9746
                     /gene="rsxB"
                     /locus_tag="JQR05_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY5325244.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDESNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9739..>11516
                     /gene="rsxC"
                     /locus_tag="JQR05_003157"
     CDS             9739..>11516
                     /gene="rsxC"
                     /locus_tag="JQR05_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY5325245.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KAHKLEQQQANAEPEEQVDPRKAAVEA"
BASE COUNT         2680 a         2970 c         2905 g         2961 t
ORIGIN      
        1 ttcacccagc aattggttac ccaatagccg tgatttggct gccagttgtt tagcagagac
       61 gttgatgaat tgcacacgct atcccggtaa acaacacata cgaccgttta ccggttcgtt
      121 agtgtattaa ttgagctcct gtaccccacc accggagacg gtgagaattt gtccgcttac
      181 ccagctggct gcaggcgagc aaaggaacag cgccgcgtta gcaatatctt gcggttggcc
      241 cagacgtctg atcggcgtgt gctgtaacat tttttgttca atttctggtg taataacgga
      301 tttcagggca tcggttaata ttgcccccgg cgcaatgcca ttcacccgaa tatttttttc
      361 acccaggtca aatgccatat ttctgaccag atgactggcc gcagctttag atgatgcata
      421 ggaagtcatg tttatatttt tattttctgc cgccatagaa gtgatggtca gaataacgcc
      481 accgccattt ttttccattt ctggcgcaac aagttgtgac agatggaaaa aagaaaacac
      541 attcagttca taagcacggc gaaaatctgc cattggcata tcaaacggtt taggaccacc
      601 gccaccggcg ttgttaacca ggatatcaac tttacccagt ttactgacgg caaagtctgc
      661 cagtgcagag agttcctgtt cggaagtaat atcacaacgc caggcaaatg cctgaccacc
      721 cagttgttga atttcgtcta caacatggtt agctgcgtcg gcgttaatat cactgaccac
      781 cacagatgcg ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac ctgcacccgc
      841 acctgtgatg atggcgcatt ttccgtcgag tctcaggttg tcagaattaa acacataaac
      901 ctcctgtgaa cttcagtaaa aataaaaggc accgcctgtg tgccacattt tttatcatta
      961 accttgagga aaggacacta ccaagtatag cagataaaga gaggattatt tcgccgcaat
     1021 gagccgggcg ggaataataa gattgcgtga atgcgtctct tcatgggtga ttttttgcat
     1081 catgcgatcc gcaagtgtga taccaagttc ccgcgctggc gtgctggccc aggtaacagg
     1141 tatatcatca agtgtggctg gtgtcgcatc ggtaaatgcc gccagcgaaa cctgttgctc
     1201 aaagtaacga tcgactccgc tttccccgct ttgccgccct gctttcagca aaccaaacca
     1261 tgcccccatc gcaatagttt cgttatagca aaccacggca ctgatggtcg ggttatgacg
     1321 taaaagcgcc gtgatagctt ccgcggcttg cttctggctg gaagtgcact ccaacaccca
     1381 atcgctgtga aacggcaggc caaattttag tagagttgca caatagcccc ccacccgttc
     1441 tgcacgggtt aatgaggaac tttgccctcc cagccaggcg atccgctgat gcccattgcg
     1501 aatgagatgc tccgtcaaca actgtgcagc ctgcatgttg tccgggcgaa ccgtatcaac
     1561 atcatcaaga taactggcac gggaagcgaa aatcaccggg atagcttttt cttctgccat
     1621 ccgtcgcagg tcatcgctgc ttcctgcagc cccggcaatt accacaccat cgacaccctg
     1681 attcagtaac agtgaaaacc gctgtgccag ctgctcgccg tctttaccgc cgtgaagcaa
     1741 aaaaaccatc cgtccctgcg cttccagagc ttccgtcaat ccggccgtca attcggcgta
     1801 aaacggcgca gataaatcac ggacgatcaa accaatgacg ccgctttgcc cgccgcgcag
     1861 cgccgacgcc tggcgattgc gcacaaatcc cagctcttca atggcggcgt taacgcgttc
     1921 tcctgtggcg gtagagattc gccctttgcc actaagcacc agcgaaacgg tacttaccga
     1981 cacgcccgca gccagcgcaa catcatgaat ggttattttt ttggcggtag ccatgcagat
     2041 gacctactcc ctgattatgt gacagataaa acgttttacc ttttatttat cttatacccg
     2101 ctattatcgt tgcgtaatgt gatttatgac tcactaaaat ttgataaaac gttttatctt
     2161 ctcgcgcaat ttactgaatc cagattgttc tctacgagga gtcgttttat gacggcgaaa
     2221 acagcaccga aagtcacgct gtgggagttc ttccagcagt taggcaaaac cttcatgtta
     2281 cccgtggcat tattgtcgtt ctgcggcatt atgctcggca ttggtagttc tcttagcagc
     2341 catgatgtca taaccctgat cccggtcctg ggcaaccccg tgttgcaggc tatctttacc
     2401 tggatgagta agattggctc gtttgctttt agtttcctgc ctgtcatgtt ctgtatcgcc
     2461 atcccgctgg gcctggcacg cgaaaataaa ggcgtagcgg cattcgctgg cttcgtcggt
     2521 tatgcggtaa tgaacctcgc ggtaaacttc tggttgacca ataaaggcat tctgccaacc
     2581 acagatgccg ccgttctgaa agccaataac atccagagca ttcttgggat ccagtcgatc
     2641 gataccggga tcctcggtgc ggtgatcgcc ggtattatcg tctggatgct gcatgagcgt
     2701 ttccataata tccgcctgcc ggctgcgctg gcattcttcg gcggtacgcg cttcgtacca
     2761 attatctcct cgctggtgat gggccttgtc ggcctggtga ttccattagt ctggccgatt
     2821 ttcgccatgg gtattagcgg cttgggccat atgataaaca gcgcgggtga tttcggaccg
     2881 atgctgtttg gtaccggtga acgtctgctg ttgccgtttg gtctgcatca cattctggtg
     2941 gcattaattc gctttaccga cgcaggcggc acgcaggaag tctgcggtca aaccgtcagc
     3001 ggcgcactga ccatcttcca ggcgcaattg agttgcccga ccactcacgg tttttctgaa
     3061 agcgtcacgc gtttcctttc gcaaggtaaa atgcctgcgt ttctcggcgg tctgccaggt
     3121 gcagcgttag ctatgtatca ctgcgcgcgc ccggaaaatc gccataaaat taaaggtctg
     3181 ctgatttctg gcctgatcgc ctgcgtcgtt ggcggcacta ccgaaccgct ggaattcctg
     3241 ttcctgttcg tagcgccagt tctgtatgtc atccacgcgc tgttaaccgg cctcggcttc
     3301 accgtcatgt ctgtgctcgg cgtcaccatc ggtaataccg acggcaatat catcgacttc
     3361 gtggtgttcg gtattttgca tggtctgtca accaagtggt acatggtgcc agtggtggcg
     3421 gcaatctggt ttgtcgttta ctacgtcatc ttccgtttcg ctatcacccg cttcaatctg
     3481 aaaaccccgg ggcgcgatag cgaagttgcc agctcaatcg aaaaagccgt tgccggtgcg
     3541 ccgggtaaat caggttacaa cgttcctgca atcctcgaag cattaggcgg tgccgacaat
     3601 attgtcagcc tcgataactg cattacccgt ctgcgtttgt ctgtggaaga tatgtcgctt
     3661 gttaatgtgc aggcactgaa ggacaatcgg gcaattggcg tagtacaact taatcaacat
     3721 aacctgcagg ttgttatcgg gccacaagtt cagtcagtaa aagatgaaat ggccggtctg
     3781 atgcatactg tccaggcata aggataagat atgttcgatt tttcaaaggt cgtggatcgt
     3841 catggcacat ggtgtacaca gtgggattat gtcgctgacc gtttcggcac tgctgacctg
     3901 ttaccgttca cgatttcaga catggatttt gccactgccc cctgcattat cgaggcgctg
     3961 aatcagcgcc tgatgcacgg cgtatttggc tacagccgct ggaaaaacga tgagtttctc
     4021 gcggctattg cccactggtt ttccacccag cattacaccg caattgatac gcagacggtg
     4081 gtgtatggcc cttctgtcat ctatatggtt tcagaactga ttcgtcagtg gtctgaaaca
     4141 ggtgaaggcg tggtgatcca cacgcccgcc tatgacgcat tttacaaggc cattgaaggt
     4201 aaccagcgca cagtaatgcc cgttgcttta gagaagcagg ctgacggctg gttttgcgat
     4261 atgggcaagc tggaagccgt tttggcgaaa ccagaatgta aaattatgct cctgtgtagc
     4321 ccacagaatc ctaccgggaa agtgtggacg tgcgatgagc tggagatcat ggctgacctg
     4381 tgcgagcgtc atgatgtgcg ggttatttcc gatgaaatcc atatggatat ggtctggggc
     4441 gagcagccgc atattccctg gagtaatgtg gcgcggggag actgggcgtt gctgacgtcg
     4501 ggatcgaaaa gtttcaatat tcccgcgctg accggtgctt acgggattat agaaaatagc
     4561 agcagccgtg atgcctattt atcggcactg aaaggacgtg atgggctttc ttccccttcg
     4621 gtactggcgt taactgccca tatcgccgcc tatcagcaag gtgcaccgtg gctggatgcc
     4681 ttacgcgtct atctgaaaga taacctgacg tatatctcag ataaaatgaa cgccgcgttt
     4741 cctgaactca actggcagat cccgcaatcc acttatctgg cctggcttga tctacgtccg
     4801 ttgaatattg acgacaacgc gttgcaaaaa gcgcttatcg agcaagaaaa agtcgcgatc
     4861 atgccggggt atacctatgg tgaagaaggt cgtggttttg tccgtctcaa tgccggctgc
     4921 ccacgttcga aactggaaaa aggtgtggct ggattaataa acgccatccg cgctgttcgc
     4981 taaccccaat tgcgcaacgt aaaaaatcgt tgcgcaatcg tggattttta ccctgctttg
     5041 tttttataat ggtgcgcact tttatatcca gaaaaagagt gcgaccatga ttgataccac
     5101 cctgccatta actgatatcc atcgccacct tgatggcaac attcgtcccc agaccattct
     5161 tgaacttggc cgccagtata atatctcgct tcctgcacaa tcccttgaaa cactgattcc
     5221 ccacgttcag gtcattgcca acgaacccga tctggtgagc tttctgacca aacttgactg
     5281 gggcgttaaa gttctcgcct ctcttgatgc ctgccgccgc gtggcatttg aaaacattga
     5341 agatgcagcc cgtaacggcc tgcactatgt cgagctgcgt ttttcaccag gctacatggc
     5401 aatggcacat aagctgcctg tagcgggtgt tgtcgaagcg gtgatcgatg gcgtacgtga
     5461 aggttgccgc acctttggtg tgcaggcgaa gcttatcggc attatgagcc gaaccttcgg
     5521 cgaagccgcc tgtcagcaag agctggaggc ctttttagcc caccgtgacc agattaccgc
     5581 acttgattta gctggtgatg aacttggatt cccgggaagt ctgttccttt ctcacttcaa
     5641 ccgcgcgcgt gatgcgggct ggcatattac cgtccatgca ggcgaagctg ccgggccgga
     5701 aagcatctgg caggcgattc gtgaactggg tgcggagcgt attggacatg gcgtaaaagc
     5761 cattgaagat cgggcgctga tggattttct cgccgagcaa caaattggta ttgaatcctg
     5821 tctgacctcc aatattcaga ccagcaccgt agcagagctg gctgcacatc cgctgaaaac
     5881 gttccttgag catggcattc gtgccagcat taacactgac gatcccggcg tacagggagt
     5941 ggatatcatt cacgaatata ccgttgccgc gccagctgct gggttatccc gtgagcaaat
     6001 ccgccaggca cagattaatg gtctggaaat ggctttcctc agcgcagagg aaaaacgcgc
     6061 actgcgagaa aaagtcgccg cgaagtaaca aaaatggatg gtgcaaattg caccatccat
     6121 ttttcacgca aggcacaacg tcgcgcgatg tttggcggat tcgatgccca gctcaatcaa
     6181 ctccattacc tggattgcct ggcttgccgg aaccggattt tcaccatcgc catttaacgc
     6241 atcacgaatc gccgcatagt aagccggata attcccaggc accgtcaaca gcgtttcttc
     6301 gacacgttcc tcaccttcca cgcgggtaag tacgccatca cgcatatcgt agccccagtc
     6361 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     6421 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     6481 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     6541 cgtcatgctg accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa
     6601 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     6661 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     6721 caaacctttt agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac
     6781 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     6841 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     6901 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     6961 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     7021 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     7081 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     7141 cattgaagta tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc
     7201 tgccagcaat aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac
     7261 aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta
     7321 acatttaccc cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc
     7381 ttattgaatt aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga
     7441 gtcacattga caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc
     7501 tgcacttgtg gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa
     7561 accaggaatt tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt
     7621 tactcttttc agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt
     7681 gtaataacac aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct
     7741 ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg
     7801 caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac
     7861 ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt
     7921 ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca
     7981 ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg
     8041 cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag
     8101 gctggttgct tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt
     8161 tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag
     8221 cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt
     8281 acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata
     8341 ttatctggct gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg
     8401 aagttggcat agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc
     8461 cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca
     8521 gttaacctgt tgtcgcctgc tctggattaa cagataatag gcggcttttt tatttcaggc
     8581 cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta
     8641 ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg
     8701 atgggcatgg ggctggcaac aacgtttgtg atgacgctgg cgtctatttg cgcctggctt
     8761 atcgatacgt ggattttgat cccacttaat ctgatttacc tgcgcaccct ggcatttatt
     8821 ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg
     8881 ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc
     8941 gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt
     9001 tccgccgctg tcggtttctc gctggtgatg gtgctcttcg ccgccatccg agaacgcctt
     9061 gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt
     9121 cttatgtctc tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct
     9181 ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg
     9241 cctcccgccg ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac
     9301 cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca
     9361 gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa
     9421 ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc
     9481 ctgcgcggat ggtggctgtt attgatgaaa gtaactgtat tggctgcact aaatgtattc
     9541 aggcttgtcc ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg
     9601 atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc
     9661 aaccggtcgc agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta
     9721 tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg
     9781 gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg
     9841 cgccaggtac cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa
     9901 ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt
     9961 cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc
    10021 cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt
    10081 gaagactgct ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag
    10141 ttaatcgagc gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca
    10201 ggcgttaaat tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag
    10261 tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta
    10321 gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa
    10381 gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt
    10441 tctctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt
    10501 ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa
    10561 aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag
    10621 cgtgttgtaa ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg
    10681 gggacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg
    10741 gtgattatgg gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta
    10801 aagattacca actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa
    10861 caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa
    10921 cagttgtact ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc
    10981 gactgcattg aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa
    11041 tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca
    11101 gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc
    11161 cttgaacgac ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct
    11221 gccgctctgg cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa
    11281 gcgggcgaac gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa
    11341 gccagagcga aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca
    11401 cgtaaaactg ccgttgaagc agctatcgcc cgctccaaag cgcacaagct ggaacagcaa
    11461 caggctaatg cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagc
//
LOCUS       DADKXM010000121        11366 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.122, whole genome shotgun sequence.
ACCESSION   DADKXM010000121 DADKXM010000000
VERSION     DADKXM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.122"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            608..3031
                     /gene="pgaA"
                     /locus_tag="JQR05_003158"
                     /pseudo
     CDS             608..3031
                     /gene="pgaA"
                     /locus_tag="JQR05_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            3040..5058
                     /gene="pgaB"
                     /locus_tag="JQR05_003159"
                     /pseudo
     CDS             3040..5058
                     /gene="pgaB"
                     /locus_tag="JQR05_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            5051..6376
                     /gene="pgaC"
                     /locus_tag="JQR05_003160"
     CDS             5051..6376
                     /gene="pgaC"
                     /locus_tag="JQR05_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAY5325246.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLAGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6378..6791
                     /gene="pgaD"
                     /locus_tag="JQR05_003161"
     CDS             6378..6791
                     /gene="pgaD"
                     /locus_tag="JQR05_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAY5325247.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6841..7764)
                     /gene="phoH"
                     /locus_tag="JQR05_003162"
     CDS             complement(6841..7764)
                     /gene="phoH"
                     /locus_tag="JQR05_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY5325248.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRVHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8237..9502)
                     /gene="efeB"
                     /locus_tag="JQR05_003163"
     CDS             complement(8237..9502)
                     /gene="efeB"
                     /locus_tag="JQR05_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY5325249.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGSYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(9508..10635)
                     /locus_tag="JQR05_003164"
     CDS             complement(9508..10635)
                     /locus_tag="JQR05_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY5325250.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10693..>11366)
                     /locus_tag="JQR05_003165"
     CDS             complement(10693..>11366)
                     /locus_tag="JQR05_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY5325251.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         2974 a         2778 c         2660 g         2954 t
ORIGIN      
        1 tctcaaatag tctttttcca taaagctaca ccaatctgta ggttgtagat ccctttctat
       61 tattaataga tataagaact gtactgttgt caatatgaaa ttccgcccac aaacaatacc
      121 gatgatgata gttgctgaat gtaattatta ttgcctaagt gctttataca ttgtttgcaa
      181 caagccaggc ttgaaaatac ttataaatgg ttatagaaaa tccttataaa acattaattc
      241 atagatacat taggaataac aattaaatcc gtgagtgccg tagcgcagcc tttcatcagg
      301 actttcgaaa aatccgaaat catgcatcgg aatttactga tttaattatt ttaatcctaa
      361 tttattttga aaaaggcatt gggatttatg ccgtattcct gaagatcctc atcattggaa
      421 tggattttcg ggcgagaaaa ggattttata tggacactct gctcatcatt tcttcttctc
      481 atcatcaaca attcacgtct ctcttccgcg tttaataacg gattatgagg tgcaaaaata
      541 tctttctttt cagttacctg taattagata cagagagaga ttttggcaat acatggagta
      601 atacaggatg tattcaagta gcagaaaaag gtgcccgaaa accaaatggg ctttgaaact
      661 tcttactgcc gcatttttag cagcgagtcc cgcggcgaag agtgctgtta ataacgccta
      721 tgatgcattg attattgaag ctcgcaaggg taatactcag ccagctttgt catggtttgc
      781 actaaaatca gcactcagca ataaccaaat tgctgactgg ttacagattg ccttatgggc
      841 cgggcaagat aaacaggtta ttaccgttta caaccgctac cgtcctcagc aattaccagc
      901 gcgtggttat gcagctgtcg ccgtcgctta tcgtaacctg caacaatggc aaaactcgct
      961 tacactgtgg caaaaggcgc tctctctgga gccgcaaaat aaggattatc aacggggaca
     1021 aattttaacc ctggcagatg cgggtcacta tgatactgcg ctggttaaac ttaagcagct
     1081 taactctgga gcaccggaca aagccaattt actcgcagaa gcctatatct ataaactggc
     1141 ggggcgtcat caggatgaat tacgggcgat gacagagtca ttacctgaaa atgcatctac
     1201 gcaacaatat cccacagaat acgtgcaggc attacgtaat aatcaacttg ctgccgcgat
     1261 tgacgatgcc aatttaacgc cagatattcg cgctgatatt catgccgaac tggtcagact
     1321 gtcgtttatg cctacgcgca gtgaaagtga acgttatgcc attgccgatc gcgccctcgc
     1381 ccaatacgct gcattagaaa ttctgtggca cgataaccca gaccgcactg cccagtacca
     1441 gcgtattcag gttgatcatc ttggcgcgtt attaactcgc gatcgttata aagacgttat
     1501 ttctcactat cagcgattaa aaaagacggg gcaaattatt ccgccctagg ggcaatattg
     1561 ggttgcatcg gcttatctca aagatcatca gccgaaaaaa gcacagtcaa taatgaccga
     1621 gctcttttat cacaaggaga ccattgcccc ggatttatcc gatgaagaac ttgcggatct
     1681 cttttacagc cacctggaga gtgaaaatta tccgggcgcg ctaactgtca cccaacatac
     1741 cattaatact tcgccgcctt tccttcggtt aatgggcacg cctacgagca tcccgaatga
     1801 tacctggtta caggggcatt cgtttctctc aaccgtagca aaatatagta atgatcttcc
     1861 tcaggctgaa atgacagcca gagagcttgc ttataacgca ccaggaaatc agggactgcg
     1921 cattgattac gcgagtgtgt tacaagcccg cggttggcct cgtgcagcag aaaatgaatt
     1981 aaaaaaagca gaagtgatcg agccacgtaa tattaatctg gaggttgaac aagcctagac
     2041 agcattaacg ttacaagaat ggcagcaggc agctgtctta acgcacgatg ttgtcgaacg
     2101 tgaaccgcaa gatcccggcg ttgtacgttt aaaacgtgcg gttgatgtac ataatcttgc
     2161 agagattcgt atagctggtt caacaggaat tgatgccgaa ggcccggaca gtggtaaaca
     2221 tgatgtcgac ttaaccacca tcgtttattc accaccgctg aaggataact ggcgcggttt
     2281 tgctggattc ggttatgccg atggacaatt tagcgaagga aaagggattg ttcgcgactg
     2341 gctggcgggt gttgagtggc ggtcacgtaa tatctggctc gaggcggagt acgctgaacg
     2401 ctttttcaat catgagcata aacccggcgc gcgcctgtct ggctggtatg attttaatga
     2461 taactggcgt attggttcgc aactggaacg cctctctcac cgcgttccat tacgggcaat
     2521 gaaaaatggt gttacaggca acagtgctca ggcttatgtt cgctggtatc aaaatgagcg
     2581 gcgtaagtac ggtgtctcct gggctttcac tgatttttcc gacagtaacc agcgtcatga
     2641 agtctcaatt gagggtcagg aacgcatctg gtcttcacca tatttgattg tcgatttcct
     2701 acccagtctg tattacgaac aaaatacaga acacgatacc ccatactaca accctataaa
     2761 aacgttcgat attgttccgg catttgaggc aagtcatttg ttatggcgaa gctatgaaaa
     2821 tagctgggag caaatcttca gcgcaggtgt tggtgcctcc tggcaaaaac attatggcac
     2881 ggatgtcgtc acccaactcg gctacgggca acgcattagc tggaatgacg tgattgatgc
     2941 tggcgcaacg ctacgctggg aaaaacgacc ttatgacggt gacagagaac acaacttata
     3001 cgttgaattc gatatgacat tcagatttta aggataaata tgttacgtaa tggaaataaa
     3061 tatctcctga tgctggtgag tataattatg ctcaccgcgt gcatcagcca gtcaagaaca
     3121 tcatttatac cgccacagga tcgcgaatct ttactcgccg agcaaccgtg gccgcataat
     3181 ggttttgtgg cgatttcatg gcataacgtt gaagacgaag ctgccgacca gcgttttatg
     3241 tcagtgcgga catcagcact gcgtgaacaa tttgcctggc tgcgcgagaa cggttatcaa
     3301 ccggtcagta ttgctcaaat tcgtgaagca catcgaggag gaaaaccgct accggaaaaa
     3361 gcagtagtgc tgacttttga tgacggctac cagagttttt atacccgcgt cttcccaatt
     3421 cttcaggcct tccagtggcc tgctgtatgg gcccccgtcg gcagttgggt cgatacgcca
     3481 gtggatgaac aagtaaagtt tggcgatgag atggtcgatc gagaatattt tgccacgtgg
     3541 caacaagtgc gagaagttgc gcgttcccgg ctcgttgagg tcgcttctca tacatggaat
     3601 tctcactacg gtattcaggc taattccacc ggcagcttaa tgcctgtata tgtaaatcgt
     3661 gcatatttta ctgaccacgc acggtatgaa accgcagcag aataccggga aagaattcgt
     3721 ctggatgctg taaaaatgac ggaatacctg cgtacaaagg ctgaggtaaa tccacacgtt
     3781 tttgtttggc cttatggcga agcgaatggc atagcgatag aggaattaaa aaaactcggt
     3841 tatgacatgt ttttcaccct tgaatcaggt ttggcaaatg cgtcacaatt ggattccatt
     3901 ccgcgagtat taatcgccaa taatccctca ttaaaagagt ttgcccagca aattattacc
     3961 gtacaggaaa aatcaccaca acggataatg catatcgatc ttgattacgt ttatgacgaa
     4021 aaccgccagc aaatggatcg caatattgat gtgctaattc agcgggtgaa agatatgcaa
     4081 atatcaaccg tgtatttgca ggcatttgct gatcccgatg gtgatgggct ggtcaaagag
     4141 gtctggtttc caaatcgttt gctaccaatg aaagcagata tttttagtcg ggttgcctgg
     4201 caattacgta cccgctcagg tgtaaacatc tatgcgtgga tgccggtatt aagctgggat
     4261 ttagatccca cattaacgcg agtaaaatac ttaccaacag gggagaaaaa agcacaaatt
     4321 catcctgaac aatatcaccg tctctctcct ttcgatgaca gagtcagagc acaagttggc
     4381 atgttatatg aagatcttgc cggacatgct gcttttgatg gcatattgtt ccacgatgat
     4441 gctttgcttt cagattatga agatgccagt gcaccggcta tcacggctta tcagcaagca
     4501 ggctttagcg ggagtctgag cgaaattcga taaaacccgg agcaatttaa acagtgggcc
     4561 cgctttaaaa gtcgtgcgtt aactgacttc actttagaac ttagtgcgcg cgtaaaagcc
     4621 attcgcggtc cacatattaa aactgcacga aatatttttg cacttccggt aatacaacct
     4681 gaaagtgaag cctggtttgc acagaattat gctgatttcc taaaaagcta tgactggacc
     4741 gctattatgg ctatgcctta tatggaaggt gtcgcagaaa aatcggctga ccaatggtta
     4801 atacaattga ccaatcaaat taaaaacatc cctcaggcta aagacaaatc tattttagaa
     4861 ttacaggcac aaaactggca gaaaaatggt cagcatcagg ctatttcttc gcaacaactc
     4921 gctcactgga tgagcctatt acaactgaat ggagtgaaaa actatggtta ttatcccgac
     4981 aattttctgc ataaccaacc tgaaatagac cttattcgtc ctgagttttc aacagcctgg
     5041 tatccgaaaa atgattaatc gcatcgtatc gttttttata ttatgtctgg tgttatgcat
     5101 acccctatgc gtagcgtact ttcactctgg tgaactgatg atgaggttcg ttttcttctg
     5161 gccgtttttt atgtccatta tgtggattgt tggcggcgtc tatttctggg tctatcgtga
     5221 acgccactgg ccgtggggag aaaacgcacc agctccccag ttgaaagata atccgtctat
     5281 ctccattatc attccctgtt ttaatgagga gaaaaacgtt gaggaaacca tacacgccgc
     5341 tttagcacag cgttatgaga acattgaagt tattgccgta aatgacggtt caacagataa
     5401 aacccgtgcc atcctggatc gcatggctgc acaaattccc catttgcggg tcattcatct
     5461 ggcgcaaaaa caggggaaag ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga
     5521 atatctggtg tgcattgatg gcgatgcgtt attagaccgc gatgcggcgg catatattgt
     5581 ggaaccgatg ttgtacaacc cgcgtgtggg tgccgtaacc ggtaatcctc gtattcgaac
     5641 acgttctacc ctagtgggta aaattcaggt tggcgagtat tcctcaatta ttggtttgat
     5701 caagcgaacc cagcgtatct atggaaacgt atttaccgtt tccggtgtta ttgccgcatt
     5761 tcgtcgcagc gccctggcag aagtgggtta ctggagtgac gatatgatca ccgaagatat
     5821 tgatattagc tggaagctgc agttgaatca gtggacgatt ttttacgagc cacgggcgct
     5881 gtgctggata ttaatgcctg aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc
     5941 tcagggcggt gcagaagtat tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt
     6001 tcgaatgtgg ccgctgtttt ttgaatactg cctgacgaca atatgggcct tcacctgcct
     6061 ggccggtttc attatttacg cagtccaact tgccggtgta ccgttaaata ttgaattgac
     6121 acatatcgct gcgacacata ctgccggaat attattgtgt acgttatgtt tactgcaatt
     6181 tattgtcagc ctgatgatcg agaatcgcta tgagcataat ctgacttcat cgcttttctg
     6241 gattatttgg ttcccggtta ttttctggat gctgagcctg gcaacgacat tggtatcatt
     6301 tacacgagtc atgttgatgc ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg
     6361 gattctgaga ggttaatatg aacaatttaa ttattacgac ccgacaatca ccagtacgtt
     6421 tactggttga ttatgttgcc acaaccatct tgtggacatt atttgctttg ttcatattct
     6481 tattcgccat ggatctgctg acgggttatt actggcaaag cgaggccaga agccgacttc
     6541 agttctattt tttgctggca gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca
     6601 ataagctgcg ttttcaaaaa cagcagcatc atgcagccta ccaatatacg ccgcaagaat
     6661 atgcagagag cttagcaata cctgatgagc tctatcagca actacaaaaa agccacagga
     6721 tgagcgtaca cttcaccagc caggggcaaa taaaaatggt tgtttcagaa aaagcgctag
     6781 tccgggcata aacacccaaa acaaagcccg gttcgcccgg gctctgcacc gataatacac
     6841 ttaactgtat gcatgcagcg tacgttggca aagtgccgaa cgtacgcaat cctcttttcc
     6901 gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc
     6961 ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga taaccgtcac
     7021 gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc
     7081 gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga acggcgcggt
     7141 ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc ctaagcgccg
     7201 gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga tatcaccagg
     7261 taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa tcctgtcgac
     7321 atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc
     7381 ggcttcgccc gtggcgaata tcagctgctt actctcaata gcctgcagat agtgcaattg
     7441 cgcttcattt cgcgcaagga tgggcgaggt atcgcgactg tcgcgggcca taccaatggc
     7501 ttctacgccg ctcatctgca caagcgaggt gaccgattct tcttcacgct gcttatggct
     7561 gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg
     7621 tcttcccatg gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg
     7681 attatgcaca cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata
     7741 aaatgccgaa aaacggctac gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg
     7801 aagtgagaaa aatacagagt gacgaagttg ctaccggagg agaaatctcc gcgagtggtg
     7861 gaacgttgct taacaaaaca ttgtccagaa caggacgtta ccatccgcga tctccatagt
     7921 gactgactat cactgccggg aacttccgct gctacttaat aagtacaaca gaactcgcat
     7981 tcattgcaac aatatattca cttatattta acgataaata cccatttcgg tgacattaat
     8041 ttctactgga aagatgacag agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag
     8101 gattcgcagg gctggtaaaa tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc
     8161 cgacctacaa gtgattaata atttgtgcac aaaactcatg cccgataaac ctgcgcatcg
     8221 ggcatatttg cgtcgttcaa acctgcaata gcgcccttcc cagataatcg ttcgcgtcct
     8281 tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg
     8341 cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc
     8401 agacaaacag caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt
     8461 agccgcgacg cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga
     8521 tatggctgtc cagcgcgatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat
     8581 gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct
     8641 gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct
     8701 gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt
     8761 tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca
     8821 aattaatcgg cgtctctttg cctttactac gcgccgcgtg atcggaaata aacccttccc
     8881 gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga
     8941 tcaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga cataacgccg
     9001 catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc atctgtggcg
     9061 caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat
     9121 cgggcgcaat gtagctgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg
     9181 tttctggtgc tgctccgccc tgactcagaa aagcaaaacg ctgagtcaac aagcgaaaca
     9241 accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg
     9301 cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc
     9361 gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac
     9421 aacttcccgc cagcgcacca atccctttca gtaaacgtcg gcgtgacggt tcattcacgc
     9481 cgttttcatc tttatactgc ataacgctta atccagtccc agcacaccgc gaagttgcgc
     9541 cagatcttcc gccagcgcag taatcggccc tttcagcgca ttccggtcgg catcggtcaa
     9601 tttgtcgtag gtttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt
     9661 tttaaagttg gcatcgactt ttgccaacag ttccgggttg gctttttgca gttgtggacg
     9721 cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt
     9781 gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc
     9841 ggctgcgccg ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa
     9901 atcgaccaca tcggtataaa gctgctcagc gtactgatcc atccctttgg tggtgttgtc
     9961 gccaaacaat gctttttcca gacggtggaa accagtgaat tttgggtcgg cggctttttg
    10021 ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat
    10081 cggttcaata cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat
    10141 atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc
    10201 cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact
    10261 ttgcgccgca tccgccgttg cctcaccttt gacgatcaac ttccctttcg ggttagtcag
    10321 cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa
    10381 accaggggcg atattttccc gctcttccac caccatcacg cctttgagga tctcccactc
    10441 cagcgccttc tggctatggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat
    10501 ggtcatcggt tcgcactgct tatccgtcac ggtcactttg acctgcggca catcagcggc
    10561 gttagccata aaagcagaag aaaacagcgc agccacgctc aactgcaatg cgttacggcg
    10621 gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt
    10681 gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa
    10741 tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga cgctcggtgc
    10801 ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag ttgagaggac
    10861 cgcgctcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc
    10921 gcgaatggca ccagccgcca gccctgcggc gacgaagaga ataaacaggc tggtccattt
    10981 aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc cgagcaccac
    11041 ggcagtagca agaccgagca ttgcacccag cggcggccag atcccgacat cttgttgaaa
    11101 tgccgccagc aggaaaaaga ccgactccag cccttccctt gcaacggcaa aaaagaccat
    11161 catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctatcga ctgcctgttc
    11221 cagttgcact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat
    11281 caccacggcg atcaccgcca cgataccttc aaacagttcc tgctcttttt gcggaaattc
    11341 gccggtggtt tcgttaatga agatcc
//
LOCUS       DADKXM010000122        11292 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.123, whole genome shotgun sequence.
ACCESSION   DADKXM010000122 DADKXM010000000
VERSION     DADKXM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.123"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            61..5301
                     /locus_tag="JQR05_003166"
     CDS             61..5301
                     /locus_tag="JQR05_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325252.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTSLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALKAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNLTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSETEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGKNITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITNTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIHSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JQR05_003167"
     CDS             complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JQR05_003167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY5325253.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            6258..6941
                     /locus_tag="JQR05_003168"
     CDS             6258..6941
                     /locus_tag="JQR05_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY5325254.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            6938..7399
                     /locus_tag="JQR05_003169"
     CDS             6938..7399
                     /locus_tag="JQR05_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325255.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTA
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7412..8584
                     /gene="iadA"
                     /locus_tag="JQR05_003170"
                     /pseudo
     CDS             7412..8584
                     /gene="iadA"
                     /locus_tag="JQR05_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            8649..9560
                     /gene="hypT"
                     /locus_tag="JQR05_003171"
                     /gene_synonym="qseD"
     CDS             8649..9560
                     /gene="hypT"
                     /locus_tag="JQR05_003171"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY5325256.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9553..9945)
                     /gene="iraD"
                     /locus_tag="JQR05_003172"
     CDS             complement(9553..9945)
                     /gene="iraD"
                     /locus_tag="JQR05_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY5325257.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9942..10025)
                     /locus_tag="JQR05_003173"
     CDS             complement(9942..10025)
                     /locus_tag="JQR05_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY5325258.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            10618..>11292
                     /locus_tag="JQR05_003174"
     CDS             10618..>11292
                     /locus_tag="JQR05_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY5325259.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIDIE"
BASE COUNT         3055 a         2788 c         2694 g         2755 t
ORIGIN      
        1 gcattatatc tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact
       61 atggcttcga caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg
      121 ggtgacaaaa tacgccgcct gaccgccgga gtttgtttat ttacacaaat ttttttcccc
      181 gtcatggcga cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc
      241 ccccctctac tagaaaataa tacggtgcct tacacgcttg gtgcgctgga atcagcgcaa
      301 agcgttgctg atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt
      361 acttttgctc gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca
      421 acctcgcaga aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg
      481 ctggagaatc aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg
      541 aatagtgagc aggccagcgg catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg
      601 atgactgatt ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat
      661 tttagcctga aaaattcgca attcgacttc ctgcatccgt ggtatgacac acccgattat
      721 ctgctcttca gccagcatac ccttcaccga acagacgatc gtacccagat caacaccagt
      781 ttgggctggc gtcatttcac ctccagctgg atgtcaggca tcaacctgtt ttttgaccac
      841 gacctgagcc gctatcactc ccgcgcgggg cttggcgcag aatactggcg tgattatctg
      901 aagttgagca gcaacgctta tattggcctg accggctggc gtagcgcacc agaattggat
      961 tatgactacg aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct
     1021 gcctggccac aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg
     1081 ctgtttgaca agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat
     1141 acccccttcc cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat
     1201 gacacacgtt ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt
     1261 aatccggacg aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat
     1321 cgcaacaaca acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg
     1381 gatccagtga aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa
     1441 tatgccctga aaggctataa catcgaagcc gctgcgctga aagctgccgg aggtaaagtc
     1501 agcacgtctg gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca
     1561 gaaaccgata atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca
     1621 cggcatgaac aaagcatggt agttattcag gctccgacat taagccagaa agattctctg
     1681 ttatccgtca atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt
     1741 actgcgcacg attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa
     1801 ggcgttcagg atatcaccct gtctgactgg acagataacg gtgatggtag ttacatacag
     1861 atgctgaccg ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag
     1921 agtgcggtaa aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat
     1981 tcatcaataa caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg
     2041 gaactgaaag acgacagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc
     2101 gttactgtcg aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg
     2161 gtttatgccg cgaattatcc ggcccataag caagggactg cactaagggc acaacttagc
     2221 cttcacaact ggaatgctcc actgcaatca catatttata acattgaggc aaaccagaat
     2281 aaggctcgcg tcgccacatt atcagcgacg aataatgacg tttacgctga taaaaagaca
     2341 tttaataccc tcactatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag
     2401 gtcaccttta agaatgaaaa aggaagcgcg gaatttgtcg aaccgccgca gcaaaatacg
     2461 gatggatacg gtgttgccac aatcaacatg gtaagccagg ttgcggaaga aaacacgatt
     2521 agcgccacgc tgccaaatgg tttctcacaa cggataattg cgaaattcgt tagcgattcg
     2581 agtacgccaa aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc
     2641 cagggcagta ctctgaccgc catcgtcaca gactttcata acaacccgtt aaaagatatg
     2701 aaagtgaatt ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac
     2761 cagtccggta ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat
     2821 gcctcgcttg aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac
     2881 agggaacaat cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca
     2941 aatatcgtta ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag
     3001 gatgtgaaat ttaccttgcc agcctccatg accgggaacc tcacgctaag tagtgaaacc
     3061 gcccgcaccg atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag
     3121 tttacagtta cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt
     3181 attggggata caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt
     3241 gcgggtaaca gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg
     3301 cttaaagacc agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc
     3361 accaccaata cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat
     3421 aacgtcgtgg tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg
     3481 ctgccggatg aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg
     3541 gtgggcaaaa acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg
     3601 ggtcaacgcg tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct
     3661 tacaccgata ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat
     3721 caggtgacgg caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat
     3781 ggcaaactcg agttaacatc atcgaaacca gaaaccacgg tccataatag tgagggtatt
     3841 acgctgaccg caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc
     3901 tttagcgtaa cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag
     3961 tcaggtcagg ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc
     4021 ataatgggca aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct
     4081 aaaacggcac atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc
     4141 gatagcagca ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc
     4201 gatgttagtc atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt
     4261 accgatcagt ccgggcaagt cacggcgaca ataaccaata cattggcaga aaccttaaca
     4321 gtcaatgtgc aagttcctgg tacagccaac caatccgcaa ccattacatt ggttgccggc
     4381 acggccgatg aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac
     4441 cagcagagcg caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg
     4501 tctgatcatc tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat
     4561 attgattaca gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag
     4621 ggaacagcga ccctcattcc catgctgaac ggggttcatc aggcaaactt aagcgtatcg
     4681 ctgaatctca tccactcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc
     4741 ttctccacgg ctaaattccc gagcgaaggc tttgcagggg cgtattacac actcaacaat
     4801 gataactttg aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagagttgg
     4861 gtgtctgtcg atgcttcagg taaggtttct ttcgcaaata tcggcgatca aacgtcagtc
     4921 acaataagcg cagttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa
     4981 ggctggtggg tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt
     5041 catgctaaaa atgatggata taatcttcct ggcatcacac atttgacgtc tggcgaaaac
     5101 aaacgcacgc agggatcgct gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat
     5161 tcgcaattta caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac
     5221 tatgtgcaga tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc
     5281 gcctgccgta aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg
     5341 tatcccttca gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta
     5401 tataacccct gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca
     5461 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
     5521 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg
     5581 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc
     5641 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
     5701 gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc
     5761 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
     5821 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
     5881 tatgagctta ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg
     5941 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
     6001 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac
     6061 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
     6121 tttccctatg caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg
     6181 gctgataagg tttttcagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa
     6241 aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag
     6301 aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg
     6361 ttttttccgg aacagacagc tggtggcgcg tttttgattt ttccgttctg aacggatcgt
     6421 ttgggcaact gcctggtgcg aatggagcca ccacctcatt tcgcggggtg ggtggcgcgg
     6481 gggctaaaga tggctttctc tttgcgctgg agctggcacc gtcagtcatt ctctctctgg
     6541 ggattatttc aattaccgat ggtcttggtg gattacgcgc cgcgcagcag ttaatgacac
     6601 ctgtgctaaa accgttgttg ggcattccag ggatctgctc gctggcactg atcgctaacc
     6661 tgcaaaacac ggatgccgcc gccggtatga ccaaagagct ggcgcaggaa ggtgaaatta
     6721 ccgaacgcga taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc atcatcacca
     6781 actacttctc ttccggcgtc gcggtgtttg cctttctggg cacgtccgta atcgtacctt
     6841 tagccgtcat cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc tggcttaact
     6901 tcgaagaacg tcgcaaccca acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa
     6961 cgtcatggat atgtttatcg atggcgcgcg ccgtggcttt actatcgcca cgaccaacct
     7021 gctgccaaac gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct
     7081 cgactgggtg gggcatatct gtgaacccgt aatggcgctg tggggattac ccggtgaagc
     7141 ggcaaccgtc cttttagccg cattaatgag tatgggcggc gcagtcggtg ttgccgccag
     7201 tctggcaacc gctgacgcat taaccggcca cgatgtcacc gctttactgc ccgcaatgta
     7261 tttaatgggc aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc
     7321 caaatattac ccgcatatca ttacggtctg cgtgattaac gcattgctgt cgatctgggt
     7381 tatgcagctt attgtttaac aaggagttac catgattgat tataccgcag ccggttttac
     7441 cctgctgcag ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt
     7501 cgctaacggc aaaattatcg ccgttgccag caatatccct tctgacattg taccgaactg
     7561 cacggttgtc gatctcagcg ggcagatcct ctgcccaggt tttattgatc aacacgtcca
     7621 tttgattggc ggtggcggcg aagcaggtcc cacgacgcgc acgccggaag tggcgctaag
     7681 tcgcctgacg gaagcggacg tcacgtcagt ggttggtctg ctgggcaccg actctatctc
     7741 tcgccacccg gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc
     7801 ctagatgctg accggcgctt atcatgtccc ttcccgcact attacgggtt ccgtggaaaa
     7861 agacgtggcg attatcgatc gtgtgattgg cgtgaaatgc gccatctctg atcaccgttc
     7921 tgccgcaccg gacgtttatc acctggctaa tatggcggca gaatcccgcg ttggcggttt
     7981 gctcggcggt aaacctggcg tcaccgtgtt ccacatgggc gacagtaaaa aggcgttaaa
     8041 gcctatttat gacctgctgg aaaactgcga tgtgccgatc agcaagctgc tgccgaccca
     8101 cgttaaccgc aacgtaccgt tgtttgagca ggcactggag ttcgcgcgca aaggcggcac
     8161 catcgatatc accagcagca ttgacgaacc ggtcgcccct gccgaaggta ttgcccgcgc
     8221 cgttcaggcg ggtattccgc tggcacgcgt caccctcagc tccgacggca acggtagcca
     8281 gccgttcttc gatgacgaag ggaatttaac ccatatcggt gttgcaggtt ttgaaacgtt
     8341 gctggaaacc gtgcaggtgc tggtcaaaga ctatgatttc agtatcagcg atgccctgcg
     8401 cccgctcacc agtagcgtag ccggtttcct taacatgagc gggaaaggcg aaattctgcc
     8461 aggcaatgac gcagacttac tggtcatgac gccagaattg cgcattgagc aggtatacgc
     8521 tcgcggcaaa ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc
     8581 ttaaacggtt gtcagccgcc ctaatggacg tagaatgccc ccaagggcgg ctgacagagt
     8641 aaaacgtaat ggatgactgt ggtgcggttt tgcataatat tgaaaccaaa tggctttatg
     8701 attttctgac tctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt cgcaacgtct
     8761 cgcaaccggc attcagccgc cgcatccgtg cactggaaca ggcgattggc gttgaattgt
     8821 ttaaccgcca agtgacgcca ctgcaactct cagaacaagg gaaaatcttc cattcgcaga
     8881 tccgccatct gttgcaacag ttagaaagca accttgcaga gctgcgtggc ggcagcgatt
     8941 acgcgcaacg taaaatcaag atagccgctg cacactctct ttccctcggt ctgttaccgt
     9001 ccattatcag ccagatgccg ccgctcttta cctgggcaat tgaagccatt gatgtcgatg
     9061 aagcggtcga taaactgcgt gaagggcaaa gtgactgtat tttttccttt cacgacgaag
     9121 atttgctgga agcgccgttt gaccacattc gcttatttga atctcaattg ttccctgtct
     9181 gcgccagtga cgaacacgga gaagcacttt ttaacctcgc gcagccacac tttccgttac
     9241 tgaattacag ccgcaactcc tacatggggc gattgattaa tcgcaccctg acgcgccaca
     9301 gtgagttaag tttcagcacc ttttttgtct cttcgatgag cgagctttta aagcaggttg
     9361 ccctcgacgg ctgtgggatt gcctggctgc cggagtatgc catccaacaa gaaattcgca
     9421 gcgggcagct cgttgtgctg aatcgggacg aacaggtgat cccgattcag gcctacgcat
     9481 accggatgaa tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc
     9541 tggagattgt gcttagctga cattctcaag cgtcgcactg cgccaaccgc cattatccca
     9601 gaccagatca aacgttagcg gttcatcgtc gtggtacagc attatctcaa ggctgatcgc
     9661 cagagtgtgg cagtacgctt ctttcaaatt aacctgcaac gccgcaatcc gtggctcatg
     9721 aaataacagt gcagaacgca gctgacggca ataccagctc tggagatgtt tgtcgctcgg
     9781 gttatgcact tgccactcgg gcatgcctga aacaagtaat gacggtaatg ccgaatgagg
     9841 agaattaaaa agagtgagga gatcgctgca taccgattta cgcagcaatg acgttttatt
     9901 gcctgaaatt tcaggtaaaa cagcctgaag tgattggcgc atcattttgc gcactcctga
     9961 cgtttagcaa catatgcggc ttgccctgaa caccgggcac cactgtattg atgctcattt
    10021 tccatagcca ttcccttttg ttcattacat ccatgtcagt ctgagacagg cctgaatcct
    10081 gtcagcaaag tataaaacct tacttgccta tgaatatcta ctgacttttc aggaaaaaag
    10141 tcatgatttg ataaaaatca tcaaactcaa aaaaaaaccg ctagtatatt ctgaatattc
    10201 cgaatagtta cgtagatgaa aatacttgtc accgctgcta cttaatttcg gtgggaaatt
    10261 atttatttcc gcataatcag ctatgtatta tttgaaacaa ttagtttgta aataatataa
    10321 attgataaca tatcaaatac atcaaaaata ccattcacag gcattattaa tatagacctc
    10381 aatatagcaa catcaaattc caattagtaa taagatatgc ttttaaattg catgcaaata
    10441 attttaaatg agcgtttttt gatactttag agtgtttcct accctttaat atcactgtgt
    10501 agagttgata tcactacaac agaaacatgc atgtcatttg tttacttctc tttttactac
    10561 agaaaaattt atctattttt ctaaacatca gttaaatact cataaaaggt aactattatg
    10621 tctatgccat taagcaatgc cgctaataac caattcaata caaataatca cttccttcat
    10681 catccaaaag tcgacagtga gttaacacgt cgatatgaat atgcacgcct ggatacggaa
    10741 aatatctatc ttctgcctct tgctcgtggc aataaccata attacgatgg aaaaagcgtt
    10801 gtcgaaatta gaaaacttaa tattggcaaa cagtcctggc catttaatta tgtgacagat
    10861 acatgcagag aattcaatgg tattacaaca acaggacgta tgctctatcg aaacttgaaa
    10921 atcacctcag ctttagacga aatatatggt ggtatatgta aaaaggcgca tgctgctaca
    10981 gagttagcag aaggtttacg gctaaattta tttatgaaga gccctttcga tcctgttgag
    11041 gactatacag ttcatgaaat aacattaggc cctggttgca atgttcctgg ttatgccgga
    11101 accacgatag gatatatttc aacccttcca gcttctcagg caaaaagatg gaccaatgag
    11161 cagccaagaa ttgatatcta tattgatcag ataataactg tctcaggggt agcaaattct
    11221 tccggattcg cccttgccgc attacttaat gctaatattg agatgggaaa tgacccaatc
    11281 attgatatag aa
//
LOCUS       DADKXM010000123        11286 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.124, whole genome shotgun sequence.
ACCESSION   DADKXM010000123 DADKXM010000000
VERSION     DADKXM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.124"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..60)
                     /locus_tag="JQR05_003175"
     CDS             complement(<1..60)
                     /locus_tag="JQR05_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HAY5325260.1"
                     /translation="MNKYWLSGIIFLAYGLASPA"
     gene            complement(322..1431)
                     /gene="apbC"
                     /locus_tag="JQR05_003176"
     CDS             complement(322..1431)
                     /gene="apbC"
                     /locus_tag="JQR05_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY5325261.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1563..3596
                     /gene="metG"
                     /locus_tag="JQR05_003177"
     CDS             1563..3596
                     /gene="metG"
                     /locus_tag="JQR05_003177"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY5325262.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERTEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3737..7485
                     /locus_tag="JQR05_003178"
                     /pseudo
     CDS             3737..7485
                     /locus_tag="JQR05_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
     gene            7495..7737
                     /locus_tag="JQR05_003179"
     CDS             7495..7737
                     /locus_tag="JQR05_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY5325263.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKTLSGVMKIFHRSGSPPLRRPST"
     gene            7773..11140
                     /locus_tag="JQR05_003180"
                     /pseudo
     CDS             7773..11140
                     /locus_tag="JQR05_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         2931 a         2924 c         2766 g         2665 t
ORIGIN      
        1 tgcgggagaa gccaatccat aggcaagaaa aataattcct gacagccaat acttattcat
       61 tgaacgttat ccctgtagta aaggttatgc ctggatagaa tgagtgcata acaaactata
      121 gctgtacatc cactacacag cacgaaggat gataatgaag cattgcctgt acgatcactc
      181 gactttgtag agtttcggac gaaggtccgc agaatattcg cagtattaaa taagtgttca
      241 ttaatgcggc aaaaacaaat acaccgcatc aatataacat ctctgcggtg tatgaagaat
      301 aaccagaatg gttaataggc gttagaccgc gcggaaggaa atctcgcctg gaatgacttc
      361 accctgccag tagagctgag ctgcaacgcg gtcagccagc tggcggtaga tagcggtaaa
      421 ttcgctctcc ggacggctga taaccgttgg cgtgccttta tcgagatcct cacgtaagga
      481 gatgtgtaac ggcatctgcc ccagcagctg tgtatggtat ttttcagcca gtttctctgc
      541 accaccggta ccgaaaatcg gttcatggtg accacagtta ctgcaaatat gcacactcat
      601 gttctcgaca ataccgagca ccggaacctc gactttctcg aacatcacaa tgcctttttt
      661 cgcatcgatc aacgcgatgt cctgcggggt ggtgaccacg actgcaccag ttactggaat
      721 gttttgcgcc agcgtcaatt gaatgtcacc ggtgcctggc ggcatatcaa gcaccagata
      781 gtcgagatcc ggccacaagg tttcctgcaa catctgcatt aacgccttgc tcgccattgg
      841 tccacgccac accatcgcat tgtcgtcagt gaccagataa ccaattgaat tggttgccag
      901 gccatgagac ataatcggtg ccatgtgggt accgtccggc gaagttggac gctgattttc
      961 tgcgcccagc atggtgggaa ttgatgggcc gtagatatcg gcgtccagaa tgccgacttt
     1021 cgccccttcc gccgccagtg ccagcgccag gtttaccgcc gtggacgatt tacccacgcc
     1081 gcctttaccg gaactcaccg cgataatgtt cttcacaccg ttaattcccg gctgattctt
     1141 cacgcgtttc agagtggcta tgttatgcga cagtttccag tcgatagctt tcgccccggt
     1201 gatgcgcagc agttcggcgc tacattgctc ttttaactct tcgaacgcgc tatgccagac
     1261 aaacggcata accagttcca catgcaacgt gtcgtccatc caggcgacat ggtgcaacgc
     1321 tttcagcgtg gtgaggttat gcttcagggt tgggtgctga aaattggcca gtgtcccggc
     1381 gaccattgcg cgcaaggctt ctggtgattt ggcctgggat tgttcgttca tcccgactcc
     1441 ttttttgtat agataaacca tcagctgata gtttacctga agaatataga gaagtactta
     1501 cttaacattt tcccatttgg tactatctaa ccccttttca ctattaagaa gtaatgccta
     1561 ctatgactca agtcgcgaag aaaattctgg tgacgtgcgc actgccgtac gctaacggct
     1621 caatccacct cggccatatg ctggagcaca tccaggctga tgtctgggtc cgttaccagc
     1681 gaatgcgcgg ccacgaggtc aacttcatct gcgccgacga tgcccacggt acaccgatca
     1741 tgctgaaagc tcagcagctc ggtatcaccc cggagcagat gattggcgaa atgagtcagg
     1801 agcatcagac tgatttcgca ggctttaaca tcagctatga caactatcac tcgacgcaca
     1861 gcgaagagaa ccgccagttg tcagaactta tctactctcg cctgaaagaa aacggtttta
     1921 ttaaaaaccg caccatctct cagctgtacg atccggaaaa aggcatgttc ctgccggacc
     1981 gttttgtgaa aggcacctgc ccgaaatgta aatccccgga tcaatacggc gataactgcg
     2041 aagtctgcgg cgcgacctac agcccgactg aattgatcga gccgaaatcg gtggtttctg
     2101 gtgctacacc agtgatgcgt gattctgaac acttcttctt tgatctgccc tctttcagcg
     2161 aaatgctgca ggcatggacc cgcagcggtg cgttgcagga gcaggtggca aacaaaatgc
     2221 aggagtggtt tgaatccggc ctgcaacagt gggatatctc ccgcgacgcg ccatactttg
     2281 gttttgaaat tccgaacgcg ccgggcaaat atttctacgt ctggctggac gcaccgattg
     2341 gctacatggg ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta agcttcgatg
     2401 aatactggaa gaaagactcc accgccgagc tgtaccactt catcggtaaa gatatcgttt
     2461 acttccacag cctgttctgg cctgccatgc tggaaggcag caacttccgc aagccgacca
     2521 acctgtttgt tcatggctat gtgacggtga acggcgcgaa gatgtccaag tctcgcggca
     2581 cctttattaa agccagcacc tggctgaatc attttgacgc tgacagcctg cgttactact
     2641 acactgcgaa actctcttcg cgcattgatg atatcgatct caacctggaa gatttcgttc
     2701 agcgtgtgaa tgccgatatc gtgaacaaag tggttaacct ggcctcccgt aatgcgggct
     2761 ttatcaacaa gcgttttgac ggtgtgctgg caagcgagct ggctgaccct gagctgtaca
     2821 aaacctttac cgatgccgct gaagtgattg gtgaagcgtg ggaaagccgt gaatttggta
     2881 aagcagtgcg cgaaatcatg gcactggctg acctggctaa ccgctatgtc gacgagcagg
     2941 ctccgtgggt agtcgcaaaa caggaaggtc gcgatgctga cctgcaggcg atttgctcaa
     3001 tgggcatcaa cctgttccgc gtgctgatga cttacctgaa gccggtactg ccgaaactga
     3061 ccgagcgtac agaagccttc ctcaatacgg aactgacctg ggatggtatc cagcaaccgc
     3121 tgctgggcca caaagtgaat ccgttcaagg cgctgtataa ccgcatcgat atgaaacagg
     3181 ttgaagctct ggtggaagcc tctaaagaag aagtgaaagc cactaccgcg ccagttactg
     3241 gcccactggc agatgatccg attcaggaaa ccatcacctt tgacgacttc gccaaagttg
     3301 acctgcgcgt ggcgctgatc gaaaacgcag agtttgttga aggttctgac aaactgctgc
     3361 gcctgacgct ggatctcggc ggtgaaaaac gcaatgtctt ctccggtatt cgttctgctt
     3421 acccggatcc gcaggcactg attggtcgtc acaccattat ggtggctaac ctggcaccac
     3481 gtaaaatgcg cttcggtatc tctgaaggca tggtgatggc tgccggtcct ggcgggaaag
     3541 atattttcct gctaagcccg gatgccggtg ctaaaccggg tcatcaggtg aaataatccc
     3601 ccttcaaggc gctgcatcga cagcgccttt tctttataaa ttcctaaagt tgttttcttg
     3661 cgattttgtc tctctctaac ccgtataaat actggtagca tctgcattca actggataaa
     3721 attacaggga tgcagaatga gacactttat ctatcaggac gaaaaatcac ataaattctg
     3781 gacggtggag caacagggaa acgagttgca tatcagttgg ggaaaagttg gcaccaaagg
     3841 gcaaagtcag ataaaaagtt tttcagatgc tgcggcggcg gaaaaagcgg aactgaagct
     3901 gattgcggag aaggtgaaga aggggtatgt ggagcaagcg aaagataatt ctttgcaacc
     3961 ttcccaaacg gtaacggact ctctcaaggt agcggactta tccaccatta ttcaggaaca
     4021 accctctttc gtagcagaaa cccgtgcggc tgacaaaaat acagatgctg ttttaccttg
     4081 gctggcgaaa gatattgctg tcgtttttcc gcccgaagtt gtacacacca cgttaagtca
     4141 tcgccgcttt cccggagttc ctgttcagca agcagacaaa ttgacccaat tacgtcgctt
     4201 agcctgtagc gtgtcgcaac gggataataa aacagccaca tttgacttca gcacctgttc
     4261 tttagaatgg caaaacaccg tcgcccaagc gatcagtcag atcgacggcc tgaaaacaac
     4321 acagctacca tcaccagtaa tggctgtact cacggcactt gaaatgaaac caggaaggcg
     4381 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4441 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4501 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4561 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4621 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4681 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     4741 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     4801 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     4861 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     4921 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cgaatgacga
     4981 aagcctgcgc tgcgttccca cacgcagcac tggcagcact ggcggaactt ctagtgcaaa
     5041 aagaagagaa tagttggcgc attatgctaa tgacgatgtt tatctcgcaa ccagcactgg
     5101 cagaacaggt cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc
     5161 accaactgac acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct
     5221 ccccaccatg gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5281 taggcatcga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5341 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5401 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5461 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5521 cacctaccgg acattattgg cagttgggga ttttgacaac attgccgctg gaggctggag
     5581 aaagcagtaa aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc
     5641 atgttacatt ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg
     5701 caggaagcct tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc
     5761 cgtgccttca acaaactcaa aacgctacgc aaaaacgccc gcacctggct gttgaaatac
     5821 ccggaacatg cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacag
     5881 gataacgccc gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa
     5941 gaaatcgccc ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg
     6001 ctcgatccct tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca
     6061 tcgatctgga cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc
     6121 ctccacctcg gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg
     6181 caggtgaaag acgcctgtac cgctgactct ctggctgaat ttgcctagga tctgtttacc
     6241 gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt
     6301 ctcggtaacg atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa
     6361 tcccagcata aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc
     6421 gccctgatgc agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg
     6481 gcaaaagaaa agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa
     6541 gatcggttag caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc
     6601 ccacgccagt tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc
     6661 ggcagccgcc tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac
     6721 gatgctgtta accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag
     6781 gtagcaaggc ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa
     6841 ctcttcctgg ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt
     6901 tatagcgccg acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc
     6961 accgctgacg atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac
     7021 gttctggaaa tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa
     7081 ctgctaccac cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc
     7141 aacgccagcg aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg
     7201 ctggcaaata aaggctggat aaagggcgaa ccacaggatg gaggctggat gatcaaacct
     7261 ctcggtcgct ggtcgttaat catggaaatc gacgaaggtt ttgcggtggg tatgtcacca
     7321 gccgaactta gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcggaa
     7381 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc
     7441 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
     7501 aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaggcaaa
     7561 acaccactca gtcatcgcaa ttggccgggc gaaccagtgc ctgttatcac tgaaagcatc
     7621 attcagacac tgggtgatga attgctacaa aaagctgaga agaaaaaaac attgtctggc
     7681 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     7741 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     7801 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctagaa tatgcgactg
     7861 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     7921 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     7981 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     8041 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     8101 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     8161 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     8221 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     8281 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     8341 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     8401 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     8461 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     8521 aactgctggc gttaaaagaa ccaccagccc gcccaggtta tccaatcatc gaagacaaaa
     8581 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     8641 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     8701 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     8761 gccaaatgcc tgaaatcctc gtttcaccac catgccgtgg gagaaagaaa aagacttcca
     8821 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     8881 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     8941 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     9001 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     9061 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     9121 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     9181 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     9241 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     9301 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     9361 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     9421 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     9481 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     9541 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     9601 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     9661 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     9721 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     9781 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     9841 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     9901 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     9961 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
    10021 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
    10081 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
    10141 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
    10201 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
    10261 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
    10321 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
    10381 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
    10441 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
    10501 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
    10561 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
    10621 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
    10681 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
    10741 agaaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc cgccttttcg
    10801 ccagttgtcg cgccgcacgg tgctgctcac acctgacgag tcaaccagta acagcctgac
    10861 tcgctgggaa ggtaaatccg ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg
    10921 ggagtcaggt tatgaggacg catttgtcta tgacctgagt gagtaccggc tggtccttaa
    10981 gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgctaatga gcttccgttc
    11041 tcttcgagtg taccgtgaca ataaatccgt cacttttgcc gaacttgatg tgtttgattt
    11101 gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aacaaagcgg gcagtgatgc
    11161 gactgcccgt tatctacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct
    11221 ggttgctatt tacgattatg aggtccctgt acctgaagat ccgttttcct tcagacttga
    11281 gatcca
//
LOCUS       DADKXM010000124        11205 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.125, whole genome shotgun sequence.
ACCESSION   DADKXM010000124 DADKXM010000000
VERSION     DADKXM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.125"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            366..809
                     /locus_tag="JQR05_003181"
     CDS             366..809
                     /locus_tag="JQR05_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5325264.1"
                     /translation="MGHISRLIQDCDGIFNQTMRNTRILNNRSLRVKFLLQIRNTVSQ
                     IITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDK
                     FKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            734..1351
                     /locus_tag="JQR05_003182"
                     /pseudo
     CDS             734..1351
                     /locus_tag="JQR05_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1609..2013
                     /gene="ddpX"
                     /locus_tag="JQR05_003183"
     CDS             1609..2013
                     /gene="ddpX"
                     /locus_tag="JQR05_003183"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY5325265.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACVDNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRRPQQAQAMLWQACPDPQYVVDVTVGSNHSRG
                     TALDLTLRDDVEVVNDAKRGNVRRTPTKTNAI"
     gene            1958..2854
                     /locus_tag="JQR05_003184"
     CDS             1958..2854
                     /locus_tag="JQR05_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY5325266.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            2851..3837
                     /locus_tag="JQR05_003185"
     CDS             2851..3837
                     /locus_tag="JQR05_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5325267.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            3830..4756
                     /locus_tag="JQR05_003186"
     CDS             3830..4756
                     /locus_tag="JQR05_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5325268.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKSTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTASAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(4812..5243)
                     /gene="osmC"
                     /locus_tag="JQR05_003187"
     CDS             complement(4812..5243)
                     /gene="osmC"
                     /locus_tag="JQR05_003187"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY5325269.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            5589..5804
                     /gene="bdm"
                     /locus_tag="JQR05_003188"
     CDS             5589..5804
                     /gene="bdm"
                     /locus_tag="JQR05_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY5325270.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            5906..6043
                     /gene="sra"
                     /locus_tag="JQR05_003189"
     CDS             5906..6043
                     /gene="sra"
                     /locus_tag="JQR05_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY5325271.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            6194..7891
                     /locus_tag="JQR05_003190"
     CDS             6194..7891
                     /locus_tag="JQR05_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY5325272.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHSPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAAKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            8025..9035
                     /gene="adhP"
                     /locus_tag="JQR05_003191"
     CDS             8025..9035
                     /gene="adhP"
                     /locus_tag="JQR05_003191"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY5325273.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            9181..9465
                     /locus_tag="JQR05_003192"
                     /pseudo
     CDS             9181..9465
                     /locus_tag="JQR05_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
     gene            complement(9693..10346)
                     /gene="fdnI"
                     /locus_tag="JQR05_003193"
     CDS             complement(9693..10346)
                     /gene="fdnI"
                     /locus_tag="JQR05_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY5325274.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(10339..>11205)
                     /gene="fdxH"
                     /locus_tag="JQR05_003194"
     CDS             complement(10339..>11205)
                     /gene="fdxH"
                     /locus_tag="JQR05_003194"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY5325275.1"
                     /translation="DIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKACQVACS
                     EWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMHCEDPGC
                     LKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKCTLCVDR
                     VSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEGVGGTHV
                     MYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHYIGIGPN
                     KEVDDDEEDHHE"
BASE COUNT         2812 a         2816 c         2848 g         2729 t
ORIGIN      
        1 gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa
       61 aacttcaggt ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc
      121 gcgcgtttac ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct
      181 cgttactgga aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg
      241 aaatagatat tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa
      301 gccaggctga ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg
      361 ccgaagtggg ccatatctcc cgtctgattc aggattgcga cggtattttc aatcaaacca
      421 tgcgtaacac caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa
      481 gaaataccgt atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc
      541 acgaagtcgg tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct
      601 tcaaacgcga aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg
      661 acgttgataa attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc
      721 tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg
      781 ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc
      841 ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt
      901 gtctgatgca atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga
      961 aagcatgatg atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat
     1021 gggcgtgggt ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt
     1081 cagtcttccg gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga
     1141 aaaacgcatc ctagccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac
     1201 cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg
     1261 tcgcgttatt cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg
     1321 gatgagcagc gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt
     1381 ttcctttaag gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt
     1441 ccttccttaa ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa
     1501 agttaaaaaa tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc
     1561 tgcataagcg gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc
     1621 accgaactgg ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc
     1681 tgcgttgata acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa
     1741 gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg
     1801 gtgatttacg atgcgtatcg ccgcccacaa caagcacagg cgatgttgtg gcaagcctgc
     1861 ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg
     1921 ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg atgctaagcg aggaaacgtc
     1981 cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aaactggtat ggatgctgaa
     2041 aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgctac tgatgatgat
     2101 tttttcaccg tggctggcga cgcatgatcc caacgccatt gatttaaccg cccgcctttt
     2161 gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc tgtttagccg
     2221 cgtactggtc ggcagtcagc aatcaattct cgccggatta gtggtggtcg ccattgcggg
     2281 tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg cagacgccat
     2341 catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga caatggcact
     2401 ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta ttgtgcgaat
     2461 tcccttttat gtgcgcctgg cgcgggggca agcattagtt gtacgccagt atacctatgt
     2521 tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg atcaactggc atattttacg
     2581 taactcccta ccgccgctga tcgtgcaggc atcgctggat atcggtagcg cgattttaat
     2641 ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc
     2701 gatggtagcg aatggtcgca actatgtact cgatcagtgg tggtattgcg catttccggg
     2761 ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt ggtgatggta ttcgcgatct
     2821 gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga cattcaacag
     2881 ctgcatttga gtttccccgg ttttaacggt gatgttcacg cgctcaacaa tgtgtccttg
     2941 cagattaacc gcggcgaaat tgtcggtctg gtgggagaat ccggctcagg taaatcagtc
     3001 accgcaatgc tgattatgcg tctgctaccg acgggcagtt attgcgtaca tcgggggcag
     3061 atttcactgc tgggagaaga tgttcttaat gctcgggaaa aacagcttcg tcagtggcgc
     3121 ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg cccttaatcc gacacgtcga
     3181 ataggtcttc agatgatgga cgtaatccgc catcatcaac caataagtcg tcgggaagcc
     3241 agagctaaag cgattgccct gctggaagag atgcaaatcc cggatgccgt ggaagttatg
     3301 tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat tgcgctggca
     3361 ttctcctgcg agccgcaatt gattattgcc gacgaaccga cgacggcgct ggacgtcaca
     3421 gtacagttgc aggtactgcg tttgcttaaa cataaagcac gcgccagtgg aactgcggta
     3481 ctgttcatca gccatgatat ggccgtggtg tcgcaactgt gcgatagcgt ttacgtgatg
     3541 tatgccggaa gcgtaattga aagcggcggg acggcagacg ttatccatca tccccggcat
     3601 ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg ccagccatta
     3661 cccgccattc cagggacggt accaaacctc acccatttgc ctgacggctg cgcttttcgc
     3721 gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc cggcgctgac agcgtgtggt
     3781 gacaacaacc agcactgcgc ctgttggtat ccgcagcagg aggtcataag tgtctgacac
     3841 gttattaacg ttacgcgacg tccatatcaa ttttccggcc cgtaaaaact ggcttggtaa
     3901 atctacggaa catgttcatg ccattaatgg tattgattta cagatccgcc gtggtgaaac
     3961 cttagggatc gtcggcgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg
     4021 catgctgcaa ccgagccacg ggcagtacat ccgttcaggc tcacaacgca ttatgcagat
     4081 ggtgtttcag gacccgctct cttcgcttaa tccgcgctta ccggtgtggc gcatcatcac
     4141 agaaccgctc tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcagagga
     4201 gttggctgtg caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc
     4261 cggcgggcag cggcaacgca tcgccattgc cagagcactc tcttcgcagc ctgacgtgat
     4321 tgtgcttgat gagccaacct cggcgctgga tatctccgtg caggcgcaga tcctcaattt
     4381 actggtaacg ctacaggaaa atcacgggct gacttatgtg ctgatttcac acaatgtctc
     4441 ggtgatacgt catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact
     4501 gggcgacgcg caacaggtgt tgactgcttc ggcacatcca tacacccgat tattgctgga
     4561 ttccctcccc gccattgata aaccgctgga ggaagaatgg gcattacgta aaacggatct
     4621 gccgggaaac cgcacgttgc cgcaaggctg ttttttctac gaacgttgcc ctctggcaac
     4681 ccacggatgt gaagtccggc aatcattagc aataagggag gacggacgtg agctccggtg
     4741 ctggcgggcg ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat
     4801 ccggcaatgc tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga
     4861 gagaccgggc atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata
     4921 cccggcaccg caacttcact cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact
     4981 ttatccagcg acacatcggc ggtggtatca attgatgttg gcgtgaatcc cgcttccccc
     5041 agcattaatg aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca
     5101 gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc
     5161 acgccgctct cggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc
     5221 tgacctttct tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga
     5281 ctacgtgaga aacagcacga ggataagcag gatattccga ataaaatacg gcagaaaata
     5341 ttacttctgc acttaaatta aattcccaca atagataacc gggaatatat atctcaagag
     5401 atattgcatc aatatcaatc aactcaccac aataataata cctatcaata tcatggggtt
     5461 aaaatataaa aatcatcaac caggactaat ctcaacaacg aagcggtaaa tatttgccat
     5521 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
     5581 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
     5641 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
     5701 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
     5761 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
     5821 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
     5881 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
     5941 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggcgaca aatccagcgt
     6001 ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
     6061 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttatctgctt tatacttggg
     6121 gacgacgccc tggcggtaaa gcaaagacga taaaagcgtg ccagggatgg atattcaaaa
     6181 aagagtgagt gacatggaac caaaaacaaa aaaacagcgt tcgctttata tcccttacgc
     6241 tggccctgta ctgctggaat ttccgttgtt gaataaaggc agtgccttca gcatggaaga
     6301 acgccgtaac ttcaacctgc tggggttact gccggaagtg gtcgaaacca tcgaagaaca
     6361 agcggaacga gcatggatcc agtatcaggg attcaaaacc gaaatcgaca aacacatcta
     6421 cctgcgtaac atccaggaca ccaacgaaac cctcttctac cgtctggtaa acaatcatct
     6481 tgatgagatg atgcctgtta tttatacccc aaccgtcggc gcagcctgtg agcgtttttc
     6541 tgagatctac cgccgttcac gcggcgtgtt tatctcttac cagaaccgcc acaatatgga
     6601 cgatattctg caaaacgtgc cgaaccataa tattaaagtg attgtggtga ctgacggtga
     6661 acgtattctg gggcttggtg accagggcat cggcgggatg ggcattccga tcggtaaact
     6721 gtcgctctat accgcctgtg gcggcatcag cccggcgtat acccttccgg tggtgctgga
     6781 tgtcggaacg aacaaccaac agctgcttaa cgatccgctg tatatgggct ggcgtaatcc
     6841 gcgtatcact gacgatgaat actatgaatt cgttgatgaa tttatccagg ctgtgaaaca
     6901 acgctggccg gacgtgctgt tgcagtttga agactttgct caaaaaaatg cgatgccgtt
     6961 acttaaccgc tatcgcaatg aaatttgttc ttttaacgat gacattcagg gcactgcggc
     7021 ggtaacagtc ggcacactga tcgcagccag tcgcgcggca ggtggtcagt taagcgagaa
     7081 aaaaatcgtc ttccttggcg caggttcagc gggatgcggc attgccgaaa tgatcatcgc
     7141 ccagacccag cgcgaaggat taagcgagga agcagcgcgg cagaaagtct ttatggtcga
     7201 tcgctttggc ctgctgacgg acaagatgcc gaacctgctg cctttccaga ccaaactggt
     7261 gcagaagcgc gaaaacctca gtgattggga taccgacagc gatgtgttgt cactgctgga
     7321 tgtggtgcgc aatgtaaaac cagatattct gattggcgtc tcaggacaga ccgggctgtt
     7381 tacggaagag atcatccgtg agatgcataa acactctccg cgtccgatcg tgatgccgct
     7441 gtctaacccg acgtctcgcg tggaagccac accgcaggac attatcgcct ggaccgaagg
     7501 taacgcgctg gtcgccactg gcagcccgtt taatccagtg gtatggaaag ataaaatcta
     7561 ccctatcgcc cagtgtaaca acgcctttat tttcccgggc atcggcctgg gtgttattgc
     7621 ttccggcgcg tcacgtatca ccgatgagat gctgatgtcg gcaagtgaaa cgcttgctca
     7681 gtattcgccg ctggtcctga acggcgaagg tctggtacta ccggaactga aagatattca
     7741 gaaagtctcc cgcgcaattg cgtttgcggt tggcaaaatg gcgcagcagc aaggcgtggc
     7801 ggccaaaacg tctgctgaag ctttgcaaca agccattgac gataatttct ggcaagccga
     7861 ataccgcgac taccgccgta cctccatcta agcctgcacc cggtagtgaa ggctaccggg
     7921 ctatttccct ctcccttttt cagatctcat ccatactggg tagtggcgaa taaatctcat
     7981 ttgcctcacc tgctatgcag aacatcatcc gaaaaggagg aactatgaag actgcagttg
     8041 ttacgaagga tcatcatgtt gacgttacgg ataaaacact gcgctcactg aaacatggcg
     8101 aagccctgct gaaaatggag tgttgtggtg tatgtcatac cgatcttcat gttaagaatg
     8161 gcgattttgg tgacaaaacc ggcgtaattc tgggccatga aggcatcggt gtggtggcag
     8221 aagttggccc aggtgtcacc tcattaaaac caggcgatcg tgccagcgtg gcgtggtttt
     8281 acgaaggatg cggtcattgc gaatactgta acagtggtaa cgaaacgctc tgccgttcag
     8341 ttaaaaatgc cggatacagc gttgatggcg ggatggcgga agagtgcatc gtggtcgccg
     8401 attacgcggt aaaagtgcca gatggtctgg actcggcggc gcccagcagc attacctgtg
     8461 cgggggttac cacctacaaa gccgttaaac tgtcaaaaat tcgtcccggt cagtggattg
     8521 ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcaaag aatgtcttta
     8581 acgcaaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca accgaaatgg
     8641 gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg caggagaaaa
     8701 ccggtggcgc tcacgctgcg gtggtaacag cagtagctaa agctgcgttt aactcggcag
     8761 ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg gagtctatga
     8821 gcctggatat cccacgtctt gtgctggatg gcattgaagt ggtcggttcg ctggtcggca
     8881 cgcgccagga tctaaccgaa gccttccagt ttgccgccga aggcaaagtg gtgccgaaag
     8941 tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa gaaggcaaaa
     9001 tccgtggccg tatggtgatt gatttccgtc actaagaggt ctttgctgcg actcccatgt
     9061 tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta ttgttgcgta
     9121 ggaaggaaca ataatgatag tgttcccaga gaagtaacgc tactgagagt aaagacacta
     9181 atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact ggacgaactt
     9241 aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac ggcaagccga
     9301 ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa ttaaactttc cgtggtgatc
     9361 ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct agagtctggc agaggcagaa
     9421 aaaacggtgg ttgtgtcgcg attacgccgt ctggttacgc aataaaaaat ggcaccttat
     9481 aaagcgccat ttgaaactga tgacaaacgc aacattgcct gatgcgctac gcttatcagg
     9541 cctacacgtt cacttcaatc tattgtattt gaagggtttt catgccggat aaggcattca
     9601 cgacgcatcc gggatgaaca aagcccacgt tgtctacaat atgaaaatgg cgccttttac
     9661 agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact ctcttttttc
     9721 gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc ccagcgacgg
     9781 ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc catatacata
     9841 tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag caggctgtag
     9901 cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat caccccggtc
     9961 accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg cccggcgttg
    10021 tacttaccaa catccgccac tttgtgctca ttgcctttta atacttcgac aatgttcaac
    10081 agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac aaacataaac
    10141 atcagtgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc catctgcggc
    10201 gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat cccggacagc
    10261 gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat aaatttggtg
    10321 cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca cctccttatt
    10381 cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc
    10441 tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct tcggatcttt
    10501 cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca taacgtgcgt
    10561 accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg ctttcagttt
    10621 cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt ggatagcccc
    10681 ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat cgacgcagag
    10741 cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa acggacaccc
    10801 ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa tcccgttagc
    10861 gtactgaatg attgcaccag cagacgggca cgccttcagg cagcccggat cttcacagtg
    10921 catacagccg tctttacgga tcagccactc cagcttgcca ttctgttcgg tttcgctaaa
    10981 gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca
    11041 gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac aggctttaca
    11101 gccgatacag gtggaaacgt cgataagttt cgcgacttct gctttgtaat cacgcacctg
    11161 agaaggcggc gtgatggagt tagttgcgga ccttttgata atatc
//
LOCUS       DADKXM010000125        11141 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.126, whole genome shotgun sequence.
ACCESSION   DADKXM010000125 DADKXM010000000
VERSION     DADKXM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.126"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            206..689
                     /locus_tag="JQR05_003195"
                     /pseudo
     CDS             206..689
                     /locus_tag="JQR05_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            689..874
                     /locus_tag="JQR05_003196"
     CDS             689..874
                     /locus_tag="JQR05_003196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325276.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTILALPQSL"
     gene            817..1830
                     /gene="lsrB"
                     /locus_tag="JQR05_003197"
     CDS             817..1830
                     /gene="lsrB"
                     /locus_tag="JQR05_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAY5325277.1"
                     /translation="MHWHCRPTADDLGIAAISMNVQAAERIAFIPKLVGVGFFTSGGN
                     GAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPALKRA
                     MQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYSSPT
                     VTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAIIAPD
                     ANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISVYVA
                     DALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKENIGK
                     YDF"
     gene            1857..2732
                     /gene="lsrF"
                     /locus_tag="JQR05_003198"
     CDS             1857..2732
                     /gene="lsrF"
                     /locus_tag="JQR05_003198"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY5325278.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            2756..3046
                     /gene="lsrG"
                     /locus_tag="JQR05_003199"
     CDS             2756..3046
                     /gene="lsrG"
                     /locus_tag="JQR05_003199"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY5325279.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3085..3843
                     /gene="tam"
                     /locus_tag="JQR05_003200"
     CDS             3085..3843
                     /gene="tam"
                     /locus_tag="JQR05_003200"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY5325280.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3847..4761)
                     /locus_tag="JQR05_003201"
     CDS             complement(3847..4761)
                     /locus_tag="JQR05_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HAY5325281.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4968..6419)
                     /locus_tag="JQR05_003202"
     CDS             complement(4968..6419)
                     /locus_tag="JQR05_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY5325282.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(6646..8064)
                     /locus_tag="JQR05_003203"
     CDS             complement(6646..8064)
                     /locus_tag="JQR05_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY5325283.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVLLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(8203..8562)
                     /locus_tag="JQR05_003204"
     CDS             complement(8203..8562)
                     /locus_tag="JQR05_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY5325284.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(8562..9488)
                     /gene="glsB"
                     /locus_tag="JQR05_003205"
     CDS             complement(8562..9488)
                     /gene="glsB"
                     /locus_tag="JQR05_003205"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY5325285.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(9552..10511)
                     /locus_tag="JQR05_003206"
     CDS             complement(9552..10511)
                     /locus_tag="JQR05_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY5325286.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDPFIVLN
                     DADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDE
                     ENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTA
                     FREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFI
                     NGYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            10591..>11141
                     /gene="yneK"
                     /locus_tag="JQR05_003207"
     CDS             10591..>11141
                     /gene="yneK"
                     /locus_tag="JQR05_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY5325287.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPD"
BASE COUNT         2958 a         2793 c         2719 g         2671 t
ORIGIN      
        1 cgctcttagc atggggcagg gaataaccag gctatttctc catgcacagg ccaattatga
       61 aagaggagag gcttaagttc ttgaaccctg aacgaataac gactaccaga aaagcacttc
      121 gcatcactca gacatgattt aacataatat acattatgcg cagtaacatt gtagtagcta
      181 aattgagatg tccgtctctg actaaattag acatgtccgc gctacagtct caccacagcg
      241 attgctgctt ggcatttcag caattggttg gttgacgata attctggtgg catttatggc
      301 ctggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcccca
      361 accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta
      421 gtttgctcgg tggttccggt gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc
      481 agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat tttatcgcgg
      541 gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta
      601 atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt aaacccgctt
      661 cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg aactggctct
      721 tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc gaatgttaga
      781 tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg tcgccctacc
      841 gctgacgatc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
      901 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
      961 gcgggtaaag agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     1021 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tatcgtttct
     1081 gcggtttcgc ctgatggctt gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga
     1141 gtgctgacct gggactctga tactaaaccg gagtgccgct cttactacat taatcaggga
     1201 acgcccgccc agttgggagg tatgttggtg gatatggcgg cgcgtcaggt gaataaagac
     1261 aaagccaaag tcgcgttttt ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg
     1321 aaagaagcga aagcgaaaat cgccaaagag catccaggct gggaaattgt cactacgcag
     1381 tttggctata acgatgccac taaatcgtta caaaccgcag aaggaatatt aaaagcgtat
     1441 agcgatctcg acgccattat cgcccccgat gccaacgccc tgcccgctgc cgcacaagcc
     1501 gcagaaaacc tgaaaaatga caaagtagcg attgtcggat tcagtacgcc aaatgtgatg
     1561 cgcccgtatg tagagcgcgg cacggtgaaa gaatttggac tgtgggatgt ggttcagcaa
     1621 ggcaaaattt cagtgtatgt cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc
     1681 gacaagctgg atatccaggg cgtaggtcag gttgaagtct cgccaaacag cgttcagggc
     1741 tatgactacg aagcggatgg taatggcatc gtactgttac cggagcgcgt gatattcaac
     1801 aaagagaata tcggcaaata cgatttctga tgtgcataac ttaaccggag taagttatgg
     1861 cagatttaga cgatattaaa gatggtaaag attttcgtac cgatcaaccg caacaaaata
     1921 tcccttttac cctgaaaggt tgcggtgcgc tggattgggg aatgcagtca cgcttatcgt
     1981 ggatatttaa tccgaaaacg ggtaaaactg tgatgctggc ctttgaccat ggttattttc
     2041 aggggccgac taccggactt gaacgcattg atataaatat cgccccgctg tttgaacatg
     2101 ccgatgtatt aatgtgtacg cgcggcattt tgcgcagcgt agttcccccg gcgaccaata
     2161 agccggtggt actgcgggcg tcaggtgcga actctattct ggcggaatta agtaatgaag
     2221 ccgtggcgtt atcgatggat gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg
     2281 tttatatcgg cagcgaatat gaacatcagt cgatcaaaaa tattattcag ctggtcgatg
     2341 ccggaatgaa agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc
     2401 gcgatcagcg ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta
     2461 tcaaaaccta ttatgtcgaa aaaggttttg aacggattgt tgccggatgt ccggtaccca
     2521 ttgttattgc tggcggtaaa aaattaccgg agcgcgaggc gctggaaatg tgctggcagg
     2581 ctatcgatca gggcgcttct ggtgtggata tggggcgtaa tattttccag tctgaccatc
     2641 cggtggcgat gatgaaagcc gtacaggcgg tggttcacca taacgaaacg gctgatcggg
     2701 catatgaact ctatctgagt gaaaaacagt aactgcggat ttaaggagaa gaattatgca
     2761 cgtcacactg gttgaaatta acgttcatga agacaaggtt gacgagttta tcgaagtttt
     2821 tcgccagaac cacctgggct ctgtacagga agaaggtaat ttgcgcttcg atgtcttaca
     2881 ggacccggaa gtgaattcgc gcttttatat ttacgaagcc tataaagatg aagacacagt
     2941 ggcattccat aaaaccacgc ctcactacaa aacctgtgtc gcgaaactgg aatctttaat
     3001 gaccgggccg cgtaaaaaac gtctgttcaa tgggttgatg ccgtgaggct acaattgggg
     3061 taatgcgctg acgggagtaa aaaaatgtct gactggaacc cctctttata tctgcacttt
     3121 tccgctgaac gatcgcgtcc ggcggtggag ctgcttgcca gagtgccgct ggagaatgtc
     3181 gaatatgttg ccgatcttgg ctgtggtcca ggtaacagca ccgccctgct gcatcaacgt
     3241 tggcctgcgg ccaggataac aggcatcgac tcgtctccgg cgatgattgc tgaagcgcgc
     3301 agtgctttgc cagactgcca gttcgtggaa gcagacatcc gcaactggca accggaacag
     3361 gcactcgatc tgatttttgc taatgcctca ctgcaatggc tgcccgacca ctacgaattg
     3421 tttcctcact tggtttcttt acttaatccg cagggcgtgc tggcagtaca gatgccagat
     3481 aactggctgg agccgaccca tgtgctcatg cgcgaagttg cctgggaaca aaactacccg
     3541 gatcgcgggc gtgagccgtt ggctggcgtt catgcttact acgatatttt gagcgaagcc
     3601 ggatgtgagg tcgatatctg gcgaaccact tactatcacc agatgccgtc gcaccaggcg
     3661 attatcgatt gggtgaccgc cactggatta cgtccgtggt tacaggatct gaccgagagc
     3721 gaacagcagc tttttcttac gcgctaccat cagatgctgg aagagcagta tccactgcaa
     3781 gagaacggac agatactgct ggcatttccg cgtctgttta ttgttgcccg ccgtacggag
     3841 taattatcac gtcagctggt aatgacgatc gggaagaatt tttgctggaa tttctgcttc
     3901 atcgttcatc tgtaacaggt caatttcaat agcgttgcag atggcatcca gcggtaaatc
     3961 attgttttca gtaccgaacg gatcttccag ttcttccgcc agacaatcca gcgaaataaa
     4021 agtgtaggaa atcagcacag agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc
     4081 gaacggcagc atgatacaaa acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa
     4141 gggaattggc gtgtaggcaa tgcgctcaca gcctgccaga accgctgaaa tatcattaag
     4201 ccgatcgttg aggctaataa acaggatatc tgaaagctgt ccattgcggc gctgaaccgc
     4261 caaccattct cccattatta acaagatacg gttagctgga gagttcgaag ccagtacacg
     4321 ctgaagatct tcagtcttga gataatgagc cagcacttcc gcctgtggct gtttgcgtaa
     4381 tgtcatgcgt aaacagtggg cgaaggcgat ttgcagcctg gcaaactccc ttacacttgc
     4441 cgaatccggc aatgtcgttt ttacctctcg cagtaacgac cgtgaggcaa tcatcaactg
     4501 tccccaaagt tttcgcgctt caacgtaacg ggcgtacccg gcattattac gaaaaccaag
     4561 aaaaatggcg atggcgacac cgagaatgct gaacggtgcg agggtgaatt tgatgcccag
     4621 atgcgtgtac cagggcagca tgaatataac agcaatagaa aagagaaaat tgagtagtaa
     4681 gcgcgaggat atcttggata atactgagcc gtgccagaca aaaatacggc gcagccagtg
     4741 ttgttgtgga cgaacaatca tggttatctt caggcgtgga aaagtcgccc tattaaacgt
     4801 gattacgatc acattctcaa gacgctactt acaaattacc tacaagctta caacattaac
     4861 aggcgctgca tgtgacacct gttaatgata aaggcatata ggatgttgta actaatatag
     4921 tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta gcacaacgga
     4981 cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc aacctgctcg
     5041 accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc caacacaatc
     5101 gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag ttgctgataa
     5161 cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg ctcaccacga
     5221 tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt gccgtttgcc
     5281 ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat accgttgagc
     5341 gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt gactgcactg
     5401 gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc ttcgtagatg
     5461 gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac ggtatccagc
     5521 cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat tgccactttg
     5581 cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata tttgtccagg
     5641 cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa gtgttcagcg
     5701 gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac ttcatcgcgc
     5761 ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc ctgatccagc
     5821 cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac cagttcacgc
     5881 aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc tttatccagc
     5941 gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc cggatagctt
     6001 accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc ttcggtggtg
     6061 ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc atcgtattca
     6121 ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct gaccgcttcc
     6181 cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt gctcagtgac
     6241 ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag atcggtgtgc
     6301 tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac
     6361 tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca
     6421 atgggttccc ttctgattag tcatacaacc tgtttgaatt ggtgcgacag gttagcaaac
     6481 tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca gttttatgag
     6541 aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg gatcataaag
     6601 tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga caacttcctc
     6661 accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat acagacacgt
     6721 atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg atgcgcgacc
     6781 actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaatggttg
     6841 cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag gattcaccga
     6901 cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct tatcaccgac
     6961 aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt catgtccata
     7021 gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca gatgttgtgt
     7081 ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt
     7141 cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga tcgctgccac
     7201 gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa
     7261 catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat ttgccaccag
     7321 tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg
     7381 cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac
     7441 taccgaggca ataaccgaca caataagcgc gatggcgggc atcatctgtt ctgctttaaa
     7501 gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca acacgcccgt
     7561 tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact
     7621 gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa
     7681 tagccgtaag gcacttactg gctcatactt atttttcccc agacgcttat cccgcacgac
     7741 cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca gtaaaaccag
     7801 tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta
     7861 atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg
     7921 ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt
     7981 ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga acaaacggaa
     8041 agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg tggcaattat
     8101 ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt cgctaacagc ggcggcaaat
     8161 aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct gattcatctg
     8221 tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttaacga
     8281 aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg tcgcggtggc
     8341 gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt cattagccgg
     8401 taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc
     8461 gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag agcgaaattt
     8521 tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac cgaacgccct
     8581 aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc tgcatcatcc
     8641 agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac aataccgcca
     8701 ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc gttctggtac
     8761 ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccacaggt
     8821 tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg ggccagctct
     8881 acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacgtca
     8941 tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt
     9001 tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc gcttaagccg
     9061 cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa catatcgcag
     9121 accaccagcg caccggcatt aatgaacgga ttacgcggaa taccctgttc catttccagt
     9181 tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc
     9241 tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg
     9301 gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca
     9361 atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc
     9421 tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg
     9481 actgccactt tctactcttg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg
     9541 aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa ttcgtgtaag
     9601 ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc
     9661 gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg tgccgccatc
     9721 tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca
     9781 ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc aacggggcca
     9841 aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac cgttggcgga
     9901 tagtagttac ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgagcc
     9961 agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgcgc cattggtccg
    10021 agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc tgacacaaaa
    10081 cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc cgctgcacaa
    10141 acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg
    10201 gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt
    10261 gccgctccag cctgtgcgcc aatagccgct ccagcacgaa cacttccggt caccgtgaca
    10321 gcagcaatgc gcgagtcttt aatcatctga ctgacaccgt cgttgtcggc attcagccag
    10381 ccatatacgc cttgcgggat tcccgcatct ttaaacactt gggcaatgag ctgagcacag
    10441 cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaaca
    10501 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
    10561 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
    10621 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
    10681 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
    10741 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
    10801 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
    10861 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
    10921 agccctattt atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc
    10981 aacccaaagc ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt
    11041 ctgtttatga atgattttat tgaacaattc ccaatgatca accttggcgc ccccatcaaa
    11101 agagcgcata caccacatat tgaacccctg cctccggatc a
//
LOCUS       DADKXM010000126        11078 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.127, whole genome shotgun sequence.
ACCESSION   DADKXM010000126 DADKXM010000000
VERSION     DADKXM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.127"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            78..854
                     /gene="exuR"
                     /locus_tag="JQR05_003208"
     CDS             78..854
                     /gene="exuR"
                     /locus_tag="JQR05_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY5325288.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1199..1861
                     /locus_tag="JQR05_003209"
     CDS             1199..1861
                     /locus_tag="JQR05_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5325289.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1865..2248
                     /gene="mzrA"
                     /locus_tag="JQR05_003210"
     CDS             1865..2248
                     /gene="mzrA"
                     /locus_tag="JQR05_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY5325290.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2395..2763
                     /locus_tag="JQR05_003211"
     CDS             2395..2763
                     /locus_tag="JQR05_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY5325291.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2801..3106
                     /locus_tag="JQR05_003212"
     CDS             2801..3106
                     /locus_tag="JQR05_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY5325292.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3109..3513
                     /locus_tag="JQR05_003213"
     CDS             3109..3513
                     /locus_tag="JQR05_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325293.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3503..3802
                     /locus_tag="JQR05_003214"
     CDS             3503..3802
                     /locus_tag="JQR05_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325294.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWNAWRLVKTTLKQQQLRG"
     gene            3898..4380
                     /locus_tag="JQR05_003215"
     CDS             3898..4380
                     /locus_tag="JQR05_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY5325295.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            4450..5436
                     /locus_tag="JQR05_003216"
     CDS             4450..5436
                     /locus_tag="JQR05_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY5325296.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5729..6097
                     /locus_tag="JQR05_003217"
     CDS             5729..6097
                     /locus_tag="JQR05_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY5325297.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6304..7200)
                     /locus_tag="JQR05_003218"
     CDS             complement(6304..7200)
                     /locus_tag="JQR05_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325298.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7305..8006
                     /locus_tag="JQR05_003219"
     CDS             7305..8006
                     /locus_tag="JQR05_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY5325299.1"
                     /translation="MITTQTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8029..8193
                     /locus_tag="JQR05_003220"
     CDS             8029..8193
                     /locus_tag="JQR05_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325300.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8405..9714)
                     /locus_tag="JQR05_003221"
                     /pseudo
     CDS             complement(8405..9714)
                     /locus_tag="JQR05_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
     gene            complement(9742..11073)
                     /locus_tag="JQR05_003222"
     CDS             complement(9742..11073)
                     /locus_tag="JQR05_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY5325301.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         2803 a         2935 c         2726 g         2614 t
ORIGIN      
        1 tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc gtccgcattc
       61 cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa caacttgctg
      121 ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa ctgcctgccg
      181 aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa gccatcatca
      241 tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat gtggtttcca
      301 accagccgcg ccatcagcag gcggctgaca ataatatgga attcgccaat tacggtccgt
      361 ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc gcggcaactc
      421 aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg cgcggcgaac
      481 aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc ctggcgacgc
      541 agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc cataacccgt
      601 actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg tgtgatgacc
      661 acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag ctggcaatgt
      721 ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac gatttcgagt
      781 tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat acggccacca
      841 gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt gtctcgtcgg
      901 taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc ccctgcaatc
      961 agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga cctttgttac
     1021 aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt tttaacaggg
     1081 aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca gcaataacat
     1141 tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat
     1201 ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa
     1261 tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc ttgaaaatgg
     1321 cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg gcgtgttgat
     1381 tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg ccgccagcct
     1441 cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa
     1501 ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca
     1561 cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac tgctgccgac
     1621 gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact ggatgagcgg
     1681 tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa cgccggtatt
     1741 tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt
     1801 gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta
     1861 agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca ctgttttact
     1921 tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc
     1981 gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct ggcatcacct
     2041 tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac
     2101 atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca
     2161 tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt
     2221 acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat cactcacttt
     2281 ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt
     2341 gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa
     2401 taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc
     2461 ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa
     2521 aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca
     2581 gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg
     2641 gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgataa gattgccaaa
     2701 cgcgaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2761 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2821 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2881 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     2941 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3001 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg
     3061 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3121 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3181 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3241 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3301 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3361 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3421 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3481 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3541 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     3601 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3661 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3721 ctgggccaga cgcggttttg gcgtatggaa cgcctggcgt ctggttaaaa cgaccctcaa
     3781 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     3841 aattttttga atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3901 attctctcca tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc
     3961 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta
     4021 gcgcacattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4081 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg
     4141 attctgcttg agttcggtgg tggtctggca atcctgttcg gtttcctgac tcgcaccaca
     4201 gccctgttta ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct
     4261 gaaggcgtca actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg
     4321 ctggcaatta ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa
     4381 gcacgcgcta tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag
     4441 gagagaaaaa tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa
     4501 tctaccggcg gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat
     4561 ggcgcacctg gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc
     4621 tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga
     4681 ctggaaccgt ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc
     4741 tttgatgaca gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat
     4801 cagctttatc tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg
     4861 gacaaaaaga accacaccat cgtcagcaac gaatcagcag aaatcatccg catgttcaat
     4921 accgcgtttg atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg
     4981 aaaattgacg aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct
     5041 ggttttgcca ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg
     5101 gcgcgactgg agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa
     5161 gccgatattc gcctgtggac tacgctggtg cgttttgatc cagtgtatgt gacccacttc
     5221 aagtgtgata agcaccgcat cagcgattac ctgaatctgt atggcttcct gcgcgatatc
     5281 taccagatgc cggggatcgc cgaaactgtc aatttcgatc acatccgtaa tcattacttc
     5341 cgcagccata agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc
     5401 gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct
     5461 ctgataatta gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac
     5521 ctacaaagcg tttatattca atatgttaca atgaccgcgt aggccagata agcgtagcgc
     5581 atctggcgat tttgcgcttg ccatcagtct tagcgcgcct tcacggcact ttattaattc
     5641 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     5701 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta
     5761 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     5821 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg
     5881 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcggttca
     5941 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgctat tcttaatccc
     6001 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     6061 ccgctttgga ccagatccaa aactggaaca agaataatga ttaagcacct tgtaaaaggt
     6121 gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga tgcggcgtaa acgccttatc
     6181 cagcctacaa attgccaaaa ttcaatatat tgcagtgact gcgtaggcct gataagcgta
     6241 gcgcatcagg cagttttgcg tttgtcagca gtcagaagca ccttgtgaaa ggtgcttaat
     6301 agattatttt ccagcgaaca actttggaat ttcccgcaga caccaggatt tcgcttcccc
     6361 catactgtca cgacgccagg ccataataat atcgatctcg ctggtcgatt ccgggctgac
     6421 gacacgcaac cgcccttccg caatatcttt ttcgaccatc ggatacggca tcgtcgccac
     6481 gccaagcccc gccagtaatg cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg
     6541 ctgtttgtcc agcagctgta cggtcaacac cgggcgctca cgagcggtat ccgctaccgc
     6601 aattccacga tatttcacac gcgtcacttc agataacggt tccggctcct gatgaatcgg
     6661 gtgatcaggc gcggcaacgt agacgttcat taacgtatag agcttgcgcg agttgatctc
     6721 cgacgaggaa cgaaaatgca tatccggcgc gataacgata tccgcccgcc cctgctccag
     6781 ccgttcccac gcccccgcca gcacttctgt gatgattgcc agttgggtat tggcttttgc
     6841 cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat
     6901 ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca tcagtagtca gtttatctgc
     6961 ggcttccagc aaaacgcgcc ctcgctccag caacatcctc ccgacattgg tgaatttggt
     7021 gcgatggccc gagcggtcaa acagcaccac atccagctct tcttccagtt tttgcatggt
     7081 gtagctaagt gcggaaggca cgcgtcccag ctcatccgct gccgccgcaa aactgccccg
     7141 gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat
     7201 ttcgttctca ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg
     7261 tccagcccct taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccaaa
     7321 ctgccaggca gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct
     7381 ttggacacta cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg
     7441 aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa
     7501 atgtgattct ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca
     7561 gcgccggaga ggcgttgctg ctctctaccc agccgggtgt cagctatagc gaacacaatc
     7621 tcagcaaaga caaaccgtta acgcgaatgc agctttggct ggatacctgc ccgcagcgag
     7681 agaatccgct gattcaaaag ctggcgctta atatggacaa gcagcaatta atcgcctcgc
     7741 cagagggtgc gatgggaagc ctgcaattac gccaacaagt gtggctgcac catatcgtgc
     7801 tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga
     7861 ttcacgggaa atttcatgcg cttacgcatc atgaagagaa agcggcgcta acctgcggtg
     7921 atggggcgtt tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg
     7981 ctttgctgat agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa
     8041 tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga aagaacctgc tcgcaccgcc
     8101 aaaaattttg gctatgaaga gatgttgagc gagctggaag ctatcgtcgc ggatgctgaa
     8161 acgcgtttag ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgtaaac
     8221 gccttatccg gcctacaaaa cacaacaaca tcaacaaatt aaacaccccg taggccggat
     8281 aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca caacaacatc aacaagttga
     8341 gcaccccgta ggccggataa ggtgcgccag catcgcatcc ggcacaatac tcaaaacctg
     8401 gcgtttatct ggccttgctc gccataatct cgataatctg ccgatccgtt tgctgcatcg
     8461 aatggcttgc taacgcacac aggttggcaa tcgactgctc aacaccatgc gccacgatcc
     8521 cttcattgcc ggtcacggcg gtatcatcca gcgccattaa caccgctttc cacgcagccg
     8581 aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc accatcgcaa atcatgccgc
     8641 tgacatcgcc gatcatactg ctgatcgcca tcgagatagt ttcataacgc ccatccacca
     8701 gccatgccat cccggcggcg gccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa
     8761 acgcggtaac tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc
     8821 cagtcgttca tcatccgctc cgaagtgttc tgctacaacc accacaggca ttgttgcggt
     8881 gatcccctgg ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg
     8941 cgcatcggat gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa
     9001 accgcgctcg cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa
     9061 accttcctgc gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc
     9121 aaacgggact tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt
     9181 cagcggagac tcttgctcgc cctctgccac acacgcctgc tgggtaaaca ccacaccatt
     9241 gtgcgtctca atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt
     9301 ctcaccgttc cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt
     9361 aacagagact ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc
     9421 gtctttcagc acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc
     9481 aatcggcagc cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag
     9541 atttggcgaa acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac
     9601 cgccgccgcc agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc
     9661 ctgaacggcg aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa
     9721 aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa
     9781 accagtgacg ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt
     9841 gtaaaccagc cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga
     9901 ggtgaagctc aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa
     9961 aatcatgatg ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg
    10021 caggatgttc attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa
    10081 tgcagtcatg acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc
    10141 gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt
    10201 taccgcttcg tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac
    10261 aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat
    10321 tgatttttcc cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat
    10381 cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg
    10441 tagcgaaccg acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac
    10501 taccagcagc ttggtcagca ccatgccggt cgaaattttg aataacaatt tctcgccgcg
    10561 tgaagagatc gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag
    10621 caacccttcc gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt
    10681 ggaatagacg aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc
    10741 ccagttttta cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc
    10801 tgccagcgta ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga
    10861 tgagagcaaa aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc
    10921 cgcgccaatc gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt
    10981 gatcgcttct cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc
    11041 tgcaatgacg cctttattcg atgcaatttc cataattt
//
LOCUS       DADKXM010000127        11013 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.128, whole genome shotgun sequence.
ACCESSION   DADKXM010000127 DADKXM010000000
VERSION     DADKXM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.128"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            390..1604
                     /locus_tag="JQR05_003223"
     CDS             390..1604
                     /locus_tag="JQR05_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY5325302.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLLNW"
     gene            1671..2954
                     /locus_tag="JQR05_003224"
     CDS             1671..2954
                     /locus_tag="JQR05_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325303.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3206..4123
                     /gene="oxyR"
                     /locus_tag="JQR05_003225"
     CDS             3206..4123
                     /gene="oxyR"
                     /locus_tag="JQR05_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY5325304.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(4106..5506)
                     /gene="sthA"
                     /locus_tag="JQR05_003226"
     CDS             complement(4106..5506)
                     /gene="sthA"
                     /locus_tag="JQR05_003226"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY5325305.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5827..6477
                     /gene="fabR"
                     /locus_tag="JQR05_003227"
     CDS             5827..6477
                     /gene="fabR"
                     /locus_tag="JQR05_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY5325306.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6477..6836
                     /locus_tag="JQR05_003228"
     CDS             6477..6836
                     /locus_tag="JQR05_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY5325307.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6876..7976)
                     /gene="trmA"
                     /locus_tag="JQR05_003229"
     CDS             complement(6876..7976)
                     /gene="trmA"
                     /locus_tag="JQR05_003229"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY5325308.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      8090..8280
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8345..10189
                     /gene="btuB"
                     /locus_tag="JQR05_003230"
     CDS             8345..10189
                     /gene="btuB"
                     /locus_tag="JQR05_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY5325309.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            10134..10991
                     /gene="murI"
                     /locus_tag="JQR05_003231"
     CDS             10134..10991
                     /gene="murI"
                     /locus_tag="JQR05_003231"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY5325310.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLLA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2534 a         2595 c         2922 g         2962 t
ORIGIN      
        1 tctggtgacc ttaaatttaa cgcccaacca gaaccattat ttgtcacatt cctttttatt
       61 attgtgtctt ctttacctcc actctcacca tgaaaataat attttcaaat acatcaagca
      121 atacacattt taattaattg aatataatta atttctattt ctgtgctcat tttcaatgat
      181 taatggagtt tgttatgtga ttatttaacg tgttgtttta acaataatgg ttaattattc
      241 aggtggtttt tgagggtatt ttttctcctc ggtggaaatt ttttgttttt aatgtgaaca
      301 cggttgaata atcgcttttt ctctcttgta tggtagtagg cgtaaaatta agtttgaaac
      361 gtctatttac aacacaggag attgcaataa tgaaaattat tgctgcagat gtttttgtta
      421 cttgcccagg gcgtaatttc gttacgctta aaataaccac tgaaagcgga ctttgtggat
      481 taggagatgc cacgttaaat ggtcgcgaat tgtcggtcgc ttcttatctg aaagatcatc
      541 tttgtccaca actaattggc cgcgatgcca gccgtatcga agatatctgg caattctttt
      601 ataaaggtgc ttactggcga cgtggccccg tcacgatgtc agccatttct gccattgata
      661 tggcgttgtg ggatatcaaa gctaaagcgg caaatatgcc attgtatcaa ctgcttggtg
      721 gtgcttctcg tgaaggcgtg atggtttatt gccacaccac cgggcgtacg attgatgaag
      781 tccttgaaga ctatgcaaag caccagcaga tgggctttaa ggcgattcgt gtgcagtgcg
      841 gtgtaccggg tatgcaaacc acttatggcc tggcgaaggg caaaggactg gcgtatgaac
      901 cggcaaccaa aggcctgtgg ccagaagagc agttgtggtc ttcagaaaag tatctcgatt
      961 ttacgccgaa gttattcgaa gcggtgcgta ataagttcgg ttttaacgaa catctgttgc
     1021 atgacatgca ccatcgcctg acccccatcg aagcggcgcg ctttgggaag agcattgagg
     1081 attaccgcct gttctggatg gaagatccga cgcctgccga gaaccaggaa tgttttcgtc
     1141 tgattcgcca gcataccgtg acgccgattg cggtcggtga agtgtttaac agtatctggg
     1201 actgcaagca gttaatcgaa gagcagctga ttgactatat tcgcacgacg atcacccatg
     1261 caggcggcat taccgggatg cggcggattg ccgattttgc atcgctttat caggtacgta
     1321 ccggttcgca tggcccttca gatttatcgc ctgtttgtca tgcggcggcg ctgcattttg
     1381 acctatgggt accaaatttt ggcgttcagg agtacatggg ctattccgaa caaatgcttg
     1441 aagttttccc gcacagctgg cgcttcgagg aaggttatat gcacccaggc gatgaaccgg
     1501 ggctgggtat ttctttcgat gagaaactgg cggcgaaata tccgtatgac ccggcgtatc
     1561 tccccgtcgc acgactggaa gatggcacat tgttgaactg gtaataacgg ttcgcttaat
     1621 tattttctga acagtcatca aatcgattaa ttccgctcaa ggagctaaaa atggacgttg
     1681 atgtgtcaac cagtgttgcg ggtaataaac cgcaacggat tcgtcgtatt cagacagtga
     1741 ccctggtttt attatttatg gcggggatcg ttaattttct cgaccgttcg tcattgagcg
     1801 tggcaggcga agcgattcgt ggcgagttag gattatcggc gacagagttt ggtgttttgc
     1861 tttctgcatt ttctctgtct tatggttttt cacaactacc ttccggtatt ttgctagacc
     1921 gctttggccc acgaatagtt ttaggcgcag gcttaatatt ctggtcatta atgcaggcat
     1981 taacaggaat ggttaattct tttagccact ttattttaat gcgtatcggt ctgggtattg
     2041 gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat gcacaaaaag
     2101 aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag gctatcgcgc
     2161 ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc gttatcatcg
     2221 gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac cgggcgcagt
     2281 ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg cgtccacagc
     2341 tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg atgattttag
     2401 gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc ggttatttgc
     2461 aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt cctttcctcg
     2521 cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg aaaaaaggct
     2581 acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg tcggcattag
     2641 gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt atctcaatgg
     2701 cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt
     2761 cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt gtttttgctt
     2821 cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt aatctggcgc
     2881 tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt attgtcaaag
     2941 atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag aataaaaaaa
     3001 agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact
     3061 ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc tgtgagcaat
     3121 tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta tcgccatgaa
     3181 ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc tggtggcatt
     3241 ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc agccgacgct
     3301 tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg agcggaccag
     3361 ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct
     3421 gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggcgagacga tgtccggacc
     3481 gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata ttatcccgat
     3541 gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga cccaccagtt
     3601 actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag
     3661 cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta tctatgaaga
     3721 tcacccgtgg gcgaaccgcg aatgcgtacc gatggccgat ctggcagggg aaaaactgct
     3781 gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct ttgaagccgg
     3841 ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca acatggtggc
     3901 ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc gcaaacgcga
     3961 tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg gcctggttta
     4021 tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca tccgcgcaag
     4081 aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt aacgcagcta
     4141 cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag tactcaatag
     4201 tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt tcggcagcgc
     4261 gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac aaaattttca
     4321 gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac tgggcgcggc
     4381 ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc acagagctga
     4441 tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg gtggcttcgc
     4501 cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat gccaggctcg
     4561 gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt
     4621 tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac gccagcgaat
     4681 cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt tttttacccg
     4741 acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac tcttcgttgt
     4801 gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac atctcttgat
     4861 cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca tccataccgc
     4921 ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt acatggcgcg
     4981 gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa
     5041 catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca gcggttagtg
     5101 tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt
     5161 tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt
     5221 gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg agcagtcggg
     5281 aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg gcgtgacgga
     5341 gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt tgataacgct
     5401 cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg
     5461 ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta gggcttacct
     5521 gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc caattgatcc
     5581 ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct tacttttgcc
     5641 ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag gtttccgctg
     5701 tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt cacaatactg
     5761 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
     5821 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
     5881 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
     5941 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
     6001 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgccaggcg
     6061 cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac atttatggag
     6121 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
     6181 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
     6241 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
     6301 gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga acaacgtcgg
     6361 caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc ttattactgg
     6421 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
     6481 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc ttatcgatta
     6541 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
     6601 ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc atgtcggtag
     6661 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
     6721 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
     6781 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
     6841 tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag caatacgccg
     6901 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
     6961 gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga gatatacaga
     7021 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggtggg
     7081 tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag gcggttaaac
     7141 tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
     7201 ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg tttggcgatt
     7261 tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt accgttgccg
     7321 cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
     7381 atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg acgatagatc
     7441 atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag cgcgattttg
     7501 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag tgcatcgcgc
     7561 agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag cagggaaacc
     7621 accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg
     7681 ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag ttcactggcg
     7741 gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac
     7801 aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg actgaccggc
     7861 gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg caaacgtacc
     7921 actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg
     7981 tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat gtccagatct
     8041 tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc tgtagcatcc
     8101 acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctggagctg acgcgcagcg
     8161 gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg aagtcatcat
     8221 ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg tcgcatctgg
     8281 ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt
     8341 tacaatgatt aaaaaagctt cgctgctgac ggcgtgttcc gtcacggcat tttccgcttg
     8401 ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg aacagccgcg
     8461 cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc gctggcagtc
     8521 gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc aaaacggcgg
     8581 ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg tgctggtgtt
     8641 aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc ttagccagtt
     8701 ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg ctgtttatgg
     8761 ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac ccggaacgga
     8821 aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta cgcagcaaca
     8881 actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc atggttatga
     8941 tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg gttttttaag
     9001 taaaacgctt tatggggcgc tggagcataa ctttactgat gcctggagcg gctttgttcg
     9061 cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg gttcaccgtt
     9121 gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct ataacggcga
     9181 actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca actacgatcc
     9241 ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat acaccgtcca
     9301 gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg actggcagaa
     9361 acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac gtaataccgg
     9421 catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg cagcacgcag
     9481 cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg gttgggaatt
     9541 catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac caaatctggg
     9601 gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca aacagtggga
     9661 aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat atcgtaacga
     9721 tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg aagggaaagc
     9781 gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga cacatactgt
     9841 gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt tacgccgtgc
     9901 taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact ggggtattac
     9961 ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt atcaaaccgt
    10021 taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca cctctcacct
    10081 gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag tctatggcta
    10141 ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa ccacgtccca
    10201 ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag atccggcatc
    10261 tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg tatggcgaaa
    10321 aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg caagaacgtt
    10381 atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca cttcttgcat
    10441 tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa cctgctgcac
    10501 gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt aaacgttctt
    10561 atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg ctgggctcgg
    10621 cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct ctggatgcac
    10681 taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc gttgtattgg
    10741 gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca gagggaaccc
    10801 ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta gaacatgaag
    10861 ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg acgccagaag
    10921 ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa aaactggcag
    10981 ttttaggctg atttggttga atgttgcgcg gtc
//
LOCUS       DADKXM010000128        10949 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.129, whole genome shotgun sequence.
ACCESSION   DADKXM010000128 DADKXM010000000
VERSION     DADKXM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.129"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(328..1992)
                     /gene="glnS"
                     /locus_tag="JQR05_003232"
     CDS             complement(328..1992)
                     /gene="glnS"
                     /locus_tag="JQR05_003232"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY5325311.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2195..4141)
                     /gene="nagE"
                     /locus_tag="JQR05_003233"
     CDS             complement(2195..4141)
                     /gene="nagE"
                     /locus_tag="JQR05_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY5325312.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAVGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISLVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4474..5274
                     /gene="nagB"
                     /locus_tag="JQR05_003234"
     CDS             4474..5274
                     /gene="nagB"
                     /locus_tag="JQR05_003234"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY5325313.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5334..6482
                     /gene="nagA"
                     /locus_tag="JQR05_003235"
     CDS             5334..6482
                     /gene="nagA"
                     /locus_tag="JQR05_003235"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY5325314.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6491..7711
                     /gene="nagC"
                     /locus_tag="JQR05_003236"
                     /gene_synonym="nagR"
     CDS             6491..7711
                     /gene="nagC"
                     /locus_tag="JQR05_003236"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY5325315.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7759..8511
                     /gene="nagD"
                     /locus_tag="JQR05_003237"
     CDS             7759..8511
                     /gene="nagD"
                     /locus_tag="JQR05_003237"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY5325316.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8908..10572
                     /gene="asnB"
                     /locus_tag="JQR05_003238"
     CDS             8908..10572
                     /gene="asnB"
                     /locus_tag="JQR05_003238"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY5325317.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         2739 a         2815 c         2758 g         2637 t
ORIGIN      
        1 ccagcaagat gaaagcggtt acattttatt taattatgtt ttgtgatttt aaatttattt
       61 tatggataaa ttgcgaagcg gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag
      121 cgcggcgttt gggattgttt gctatttctt gccggatgcg gcgtgaacgc cttatccggc
      181 ctacaaaagc acgcaaattc aatatattgc agagatcatg taggcctgat aagcgaagcg
      241 catcaggcaa tttagcgttt gaaatttatt gtctatttat tgccggatgc gacgtaaatg
      301 cctcacccgg cttggtagaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag
      361 cccaacggtg cggttaaata ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa
      421 gtaaccttca cgctcaaact ggaatgcttt acccgctacc gcatctttca gcgacggttc
      481 agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc
      541 agcacctggg ttaggcacgc tgaacagacg atcatacaaa cggatttcaa ccggcagcgc
      601 atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt
      661 gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattacctt cggcatcttt
      721 ctccacgcgt tcagccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac
      781 cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc
      841 accgctaaac ggcacctgac ggctccccat ttccggtttg ttcggatggt tcggcatggt
      901 gaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc
      961 cattgcgcgc ggcgcatttt cgttgagatc ttcacggatg caggattcca gcgacgccat
     1021 ctcaatggtg ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac gaatagaagc
     1081 cgcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc
     1141 ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc
     1201 cagattcagg cgcgagaatt cgtactggcg cggatgaaca ggaatggtga tgttatccag
     1261 tacccagtcg tacagacgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat
     1321 accttccagc gcatcgctga tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt
     1381 gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcattac
     1441 gataaatggc gacgccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc
     1501 accggcacgc attttttcga acagcgccaa gttctcttca acgctgcggt cgcggtacgg
     1561 gctgttttta cccggctgcg tcagggtacc gcggtattcg cggatctgtt ccggtgtcag
     1621 ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc
     1681 aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc
     1741 gtttttgatc gaatcgacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg
     1801 caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc
     1861 atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg
     1921 cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc
     1981 tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat
     2041 aagcgggaca ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg
     2101 ggatgattga tgaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg
     2161 cgacgctgcc gtgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg
     2221 tttgacccgc cacaacatgg ccctgagctt taatgatcaa gccactgaaa tcgtcgatat
     2281 tgctgcaaac caccaggcta atcatcgagc gggcgttagc gttcaggtaa tccagatcca
     2341 tttccagaat cggttgccct gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc
     2401 ctttaccttc cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt
     2461 cggtttccag gcagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg
     2521 gtgatacgac gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg
     2581 cgaatgcttc gtcaggaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca
     2641 gctccgcgat agataccgcg tttggtacag cctgcggttt tgctacaggc gcggcagttg
     2701 ccggagttgc ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg
     2761 catcgccgat ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt
     2821 tcactacacc agaagcaccc agacgtttac acatcgtatc gttgacgcgg gcagagtcag
     2881 ccacggtcag gcgcagacgg gtaatacagg catcaatcgc tttcaggttg tcagtaccgc
     2941 caaccgcagc aatatagttg gttgccagtt gagtcagacc ttcttcagta ttgctgttgg
     3001 cttcttcagt aacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca
     3061 tgcggataac caaactgaac accacgaagt agatagcgaa gaagataacg cccatcacca
     3121 gcagcatcca gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag
     3181 cccccgcaga gaaagagaag ccggcatgga tacccagcag cgttgccaca aacaggctga
     3241 taccggtcag cagtgcgtgc aggaggtaca gcagcggcgc aaggaacatg aacaggaatt
     3301 ccagcggctc agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc
     3361 caaccatcgg acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca
     3421 gaccgaacat catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccag
     3481 cgtagaaacg gttgatgtcg ccgtggaaaa ccgtacccac cgcgttggtg aattcaccaa
     3541 tctggaacca ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt
     3601 tgatgaaacc aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg
     3661 catggatagc gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc agcaccagac
     3721 agaagaagcc ggtggcgatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg
     3781 gcagtttaat atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac
     3841 ccgccagtac acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa
     3901 agtaacctac cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg
     3961 ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa
     4021 taaacgcaac gttaagtaaa tctggctgac cgaatcgcag caacagcgcc gccaccggca
     4081 gcaccgcgat agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca
     4141 tcttattccc cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg
     4201 caaattttac tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg
     4261 aaaatttaat tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat
     4321 cgttatcatc actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat
     4381 caagtcggat tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc
     4441 caacggctta cattttactt attgaggtga ataatgagac tgatccccct gactaccgct
     4501 gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca
     4561 actgccgatc gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat
     4621 aaagcgttag tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc
     4681 aacatggacg aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg
     4741 caccgtaatt tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc
     4801 aatgccccgg atatcgatgc cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga
     4861 aaaattcatc tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg
     4921 gcgtcttctc tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg
     4981 aactctcgtt tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt
     5041 gttggtacac tgctggatgc cgaagaagtg atgattctgg tgctgggtag ccagaaagca
     5101 ctggcgctgc aggccgccgt tgaaggttgc gtgaaccata tgtggaccat cagctgtctg
     5161 caactgcatc cgaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt
     5221 aagactttaa gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta
     5281 tccctgccct ggttccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg
     5341 cattaaccca gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta
     5401 tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac
     5461 aacgttctct gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct
     5521 gcggcggcgt acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca
     5581 tgcagaaagc caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca
     5641 gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga
     5701 atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc
     5761 ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg
     5821 ccgacgtcat taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca
     5881 aactggcaaa tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag
     5941 caaaagccgg tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt
     6001 atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt
     6061 gcggtattat cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc
     6121 ttaaaggcga caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg
     6181 aacagttcat ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga
     6241 acggtacgtt aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac
     6301 attgcggtat cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga
     6361 ttgacgttga aaaacgtctc ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat
     6421 tcacacctga ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat
     6481 aagagaaagt atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca
     6541 gcttaacagc gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca
     6601 gattgccgag caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat
     6661 cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat
     6721 ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga
     6781 cgccaccatc actctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct
     6841 gccggaacgt acccagcaga cgctggaaca tgccctgctg aatgccattg ctcagtttat
     6901 tgatagctac cagcgcaagc tgcgcgagct aatcgccatt tcggtgatcc tgccagggct
     6961 tgttgacccg gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg
     7021 gctggtagaa gcactggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg
     7081 tagtctggcg ctggcggagc actacttcgg tgcaagtcag gattgcgaag actccattct
     7141 ggtgcgtgtc catcgcggaa ccggggccgg gattatctct aacgggcgca tttttattgg
     7201 ccgcaacggt aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg
     7261 ccactgcggc aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg
     7321 ggtgttgaat ctgttaaagc agggctacca gagccgtgtg ccactggacg actgcaccat
     7381 caaaactatc tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag tgattgagta
     7441 tgtcggtcgt catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa
     7501 aattgttatt gctggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag
     7561 ctgcattaat acccaagcgc tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga
     7621 gctggatcac cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg
     7681 tattttgctc cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt
     7741 caatattctg ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg
     7801 atgcacgata acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa
     7861 ggcctgccgc tggtgttgct gaccaactat ccttcgcaga ctgggcaaga tctggcgaac
     7921 cgctttgcca ctgccggtgt cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg
     7981 accgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca
     8041 ctgattcatg aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg
     8101 attgttggcg aaacacgttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc
     8161 gctaacggtg cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc
     8221 gcttgtggcg cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc gttctatgtt
     8281 ggtaagccca gcccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa
     8341 gaaacagtga ttgtcggcga taacctgcgt accgatattc tggcgggctt ccaggccggt
     8401 ctggagacga ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg
     8461 ttccgcccaa gctggattta cccgtcagtc gctgaaatcg acgttatctg aataccaccc
     8521 acgtctgcgg acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt
     8581 atcaggccta cgcagctcct gcaatatatt gaattttcat gcttttgtag gccggataag
     8641 gcgttcacgc cgcatccggc atgaacaaat cgcactttat cagcaatttg gcccccgtaa
     8701 aatataacca ttcaataaaa cccgctaaaa attatcgatt catcaataaa tacatcctca
     8761 caccagaccc tttttcacca atcgacaatc accattacgt tttttatttt tgcactgcca
     8821 aatagcttgc gcacagtgct gctttgcggc atttttttaa acaagcaaac acaacaagca
     8881 acaaatacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
     8941 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
     9001 ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgctca cgaacgtctg
     9061 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aacccacgta
     9121 ctggcggtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt
     9181 tatcagttcc agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
     9241 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
     9301 gatgcctacc tgattggtcg cgaccatctg gggatcatcc cactgtatat ggggtatgac
     9361 gaacacggtc agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg
     9421 attaaagagt tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat
     9481 tatcatcgcg actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag
     9541 ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt
     9601 gtgctacttt ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgca
     9661 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt acactccttt
     9721 gctgtaggtc tgccgggttc accggatctt aaagcagccc aggaagtggc aaaccatctg
     9781 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
     9841 gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat
     9901 ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct
     9961 gatgaagtgt ttggcggtta tctttacttc cataaagcgc ccaacgctaa agaactgcat
    10021 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg cgccaacaaa
    10081 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
    10141 gtggcgatgc gcattaaccc gcaggataaa atgtgcggta acggcaaaat ggaaaaacac
    10201 atcctgcgtg aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag
    10261 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag
    10321 caggtttctg atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc
    10381 tcaaaagaag cgtatctgta ccgcgagatc tttgaagaac tgttcccact tccgagtgcc
    10441 gctgagtgcg tgccgggcgg tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg
    10501 gatgaagcgt tcaaaaaaat ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg
    10561 gcgtataagt aagaaatgcg gtactgcccc ggagaaatcc ggggctgttt tagttattgc
    10621 gctgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt
    10681 gacaactcat cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat
    10741 tccgaatttc atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta
    10801 aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaaca gtcactttcg
    10861 agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg
    10921 caacgccgat aaggtatcgc gaaaaaaag
//
LOCUS       DADKXM010000129        10859 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.130, whole genome shotgun sequence.
ACCESSION   DADKXM010000129 DADKXM010000000
VERSION     DADKXM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.130"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            83..424
                     /locus_tag="JQR05_003239"
     CDS             83..424
                     /locus_tag="JQR05_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY5325318.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JQR05_003240"
     CDS             complement(415..1341)
                     /locus_tag="JQR05_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325319.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRGAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JQR05_003241"
     CDS             1431..2429
                     /locus_tag="JQR05_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY5325320.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIADGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JQR05_003242"
     CDS             complement(2426..2644)
                     /locus_tag="JQR05_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325321.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JQR05_003243"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JQR05_003243"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY5325322.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JQR05_003244"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JQR05_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY5325323.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JQR05_003245"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JQR05_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY5325324.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JQR05_003246"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JQR05_003246"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY5325325.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JQR05_003247"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JQR05_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY5325326.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JQR05_003248"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JQR05_003248"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY5325327.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JQR05_003249"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JQR05_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY5325328.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2646 a         2802 c         2687 g         2724 t
ORIGIN      
        1 tgcgatttgt aagattaatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gccccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct gacggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcaac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caattcaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 ggggttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DADKXM010000130        10783 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.131, whole genome shotgun sequence.
ACCESSION   DADKXM010000130 DADKXM010000000
VERSION     DADKXM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.131"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            343..840
                     /gene="ubiC"
                     /locus_tag="JQR05_003250"
     CDS             343..840
                     /gene="ubiC"
                     /locus_tag="JQR05_003250"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY5325329.1"
                     /translation="MSHPALTQLRALRYFKEIPALDTQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            853..1725
                     /gene="ubiA"
                     /locus_tag="JQR05_003251"
     CDS             853..1725
                     /gene="ubiA"
                     /locus_tag="JQR05_003251"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY5325330.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1880..4303)
                     /gene="plsB"
                     /locus_tag="JQR05_003252"
     CDS             complement(1880..4303)
                     /gene="plsB"
                     /locus_tag="JQR05_003252"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY5325331.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4474..4842
                     /locus_tag="JQR05_003253"
     CDS             4474..4842
                     /locus_tag="JQR05_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY5325332.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4952..5560
                     /gene="lexA"
                     /locus_tag="JQR05_003254"
     CDS             4952..5560
                     /gene="lexA"
                     /locus_tag="JQR05_003254"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY5325333.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5579..6958
                     /gene="dinF"
                     /locus_tag="JQR05_003255"
     CDS             5579..6958
                     /gene="dinF"
                     /locus_tag="JQR05_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY5325334.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            7074..7283
                     /locus_tag="JQR05_003256"
     CDS             7074..7283
                     /locus_tag="JQR05_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY5325335.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7325..7840)
                     /gene="zur"
                     /locus_tag="JQR05_003257"
     CDS             complement(7325..7840)
                     /gene="zur"
                     /locus_tag="JQR05_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY5325336.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8158..8412
                     /locus_tag="JQR05_003258"
     CDS             8158..8412
                     /locus_tag="JQR05_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325337.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8436..9143
                     /locus_tag="JQR05_003259"
     CDS             8436..9143
                     /locus_tag="JQR05_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY5325338.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9506..9628
                     /locus_tag="JQR05_003260"
     CDS             9506..9628
                     /locus_tag="JQR05_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAY5325339.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDRMYRF"
     gene            9864..10436
                     /locus_tag="JQR05_003261"
     CDS             9864..10436
                     /locus_tag="JQR05_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HAY5325340.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHNVSLRSDPREMDADE
                     VFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
BASE COUNT         2664 a         2429 c         2794 g         2896 t
ORIGIN      
        1 ttgcctatta atcttactca ggaaacactt ttcagctcca aatctcatgg taaatatgcg
       61 cttttcccaa tttttactgc gagttggcga gctactcgta taaagaataa aggtatttaa
      121 gtaaaggaaa acatcaccgt tcctagcatc atgggcggtg atgcccctat ggttgccctc
      181 gccagcacgg gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt catgcgattg
      241 ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt aaacactttt
      301 ctgcgataca atgcctttaa gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta
      361 acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccttggatac gcaactgctc
      421 gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg
      481 agtgtgacga tgatccgcga ggggtttgtc gagcagaatg aaatccccga agaactgccg
      541 ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa
      601 ccgtggcttg ccggtcgtac cgtcgttcct gtatcaacat taagcgggcc ggaactagcg
      661 ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc gacactaacc
      721 cgggatttta tcgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga
      781 ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa
      841 gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta
      901 atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg
      961 tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtgtttgt cgcgggtgtc
     1021 tggctgatgc gcgctgctgg ttgcgtggtg aatgattatg ctgatcgcaa gtttgatggt
     1081 catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga gaaagaggcg
     1141 cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggttctgac gctgaatacg
     1201 atgaccattc tgttgtcgat tgccgctctg gcattggcgt gggtgtaccc gtttatgaag
     1261 cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg
     1321 gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat
     1381 attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga tgatgatgtg
     1441 aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt gattattggt
     1501 attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa tggcttaggc
     1561 tggggatatt actggtcaat tgtggtggct ggcgcgctgt ttgtttatca acaaaaactg
     1621 attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt
     1681 ctggtactgt ttttaggact ggcaatgagt tactggcatt tctgatgatg taaaaaagcc
     1741 ggatgatcat ccggctttct tctgggttgc ctgatgcgct gcgcttctca ggcctacccg
     1801 gtgaatcgca atttattgaa tttgcaggtc atggtaggcc ggataaggcg ttttcgccgc
     1861 atccggcaat tctctctgat tacccttcgc cctgcgtcgc actctcaatc gtcaaacgca
     1921 cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct gctggttcgg
     1981 catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga gaactgaaca
     2041 ccgccttgtc gaagaactcc ggcgcgttga tgccgtgcag cacggagaga cgttgcgcaa
     2101 cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggca ctcaacaacc
     2161 agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtaggg
     2221 tgcgagaatg cgccgggttg atatgcaact catcatcttg cagggtaatc agcccctgac
     2281 gttgcatctc atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga tcccagcgca
     2341 ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc
     2401 gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat
     2461 gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgtggc agaatgatga
     2521 tgtcgccgat agtgtctttc tcgacttcaa acttgttcat ttgcagcgcg tgatcgataa
     2581 gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg ttgcgcatca
     2641 gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag cgctgacgtg
     2701 atgccagtag cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg ttgttaatgc
     2761 gtaccatcag atcggcagca atattattga ccgtcggcgt taaccaggcc ggacgcaccg
     2821 cttcgatggg atcgatagat tcacgccagt ctggtacgtg ctggttaagg taggtcatca
     2881 acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc ttgcttaaac
     2941 cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt tctttggcgt
     3001 aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc gtaatcggac
     3061 gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt ttcggatcca
     3121 gcaaacgccc cgtgcgggaa cgaccgcctt ccacgaagta ctcgacggaa taaccacggc
     3181 tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg cctttaaaag
     3241 tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc cagaagttca
     3301 ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc acgtaagaaa
     3361 gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca tgaccgtcgt
     3421 gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa aatcggttcc
     3481 aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa ttcgccgcaa
     3541 tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc
     3601 gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta ttaaacagat
     3661 cctgacgagc aggcagacgt gggcctacgg cagccagacg ttgacgggca aagtgcatac
     3721 gcgccacgcg cgccagtttc tgagcgatag ttttatccgt gccgtgttca tccgccatac
     3781 ggcgcagcga cactgacggc gagaaacgca caaaactgtc gcgaccgagc cacagtacag
     3841 cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg cctttttcac
     3901 gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc agatttgggt
     3961 tgctacggtg caaatcgaga tagtcgtgga aaagcttaat agactcttct ttcggcgtgt
     4021 aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt
     4081 cgatttccag cggctctaac gggtcaggca aatcatgtgc cagacactgg gcgcgcaacg
     4141 tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca
     4201 gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg atgcttaatg
     4261 gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc
     4321 tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat
     4381 ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga
     4441 tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcacc
     4501 cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa
     4561 gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc ctgctggctg
     4621 gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg
     4681 gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga ataccatgag
     4741 ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgccat tatcgtcgcc
     4801 gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca gatttcgata
     4861 aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt atatactcac
     4921 agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga
     4981 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
     5041 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
     5101 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
     5161 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgcttctggc
     5221 gcaacagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga
     5281 tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgactt
     5341 gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga
     5401 tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga
     5461 aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaagg
     5521 gctggcggtt ggggttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat
     5581 gccgcctggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt
     5641 cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt tctccaatat
     5701 caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg atagtccggt
     5761 ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta tgctgttgct
     5821 gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca aaaatcctca
     5881 ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggccg gggcgttaat
     5941 tgcgctgttg cgtacgccga ttatcgatct ggcgctgcat attgttggcg gtagtgaagc
     6001 ggtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgctc cggcgtcgct
     6061 ggcgaatctg gtattactcg gttggttact cggcgtgcaa tatgcccgtg cgccagtaat
     6121 tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg tgatggggct
     6181 gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg caacattgct
     6241 gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg gcgaaatgct
     6301 gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg atatcatgct
     6361 gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg cgcgactggg
     6421 gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc
     6481 gcttgatggt tttgcctacg cggttgaagc gcactccggt caggcatacg gtgcgcgcga
     6541 cggtagccag ttgctggatg tatggcgggc agcgtgccgc cagtcgggga ttgtagcgtt
     6601 actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt
     6661 aacccagatt cagcagctgg ctgaccgcta tcttatctgg caggtgattt tgccgttggt
     6721 tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat
     6781 gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta cgctgccgtg
     6841 gctgggtaat catggtttgt ggctggcatt aaccgtcttt ctggcgttgc gcgggctttc
     6901 tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg caacgtgacg
     6961 gttaaaaatt ctgaataaat tatcctaagc caaattgctg actacactta atctcacgtt
     7021 cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata
     7081 aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca
     7141 aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg gtcggtaaaa
     7201 tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg gattgggaaa
     7261 cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa ggatgtacgc
     7321 cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac tgttcaggat
     7381 gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca atcacattat
     7441 gccgcagggc aaaccccatt tttgccgcca gcgtatgcat aatgtcttcc acgccttctg
     7501 cacactcttc tttcaccgcg ccgcagcgat cgcaaataaa catggctgac gtatgcgtgg
     7561 gctggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac
     7621 cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag
     7681 cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc agactcatca
     7741 ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg cagatttttt
     7801 cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc aaagtggggg
     7861 ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct tattgttgcc
     7921 gtaatgttga ttttctgttt tgtaggtaag gtgttgtgtt gccttgtcgt accattatca
     7981 acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac tcgctattta
     8041 tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt ttgatagtga
     8101 ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag atgaatcatg
     8161 ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc aaatcaattc
     8221 gaaaataaag atgcaaccaa agataaattg gataatagag gttcaatcaa aggtaaatta
     8281 aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga tataattatt
     8341 gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc acgtaaaaaa
     8401 atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag tatactgacg
     8461 attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt gaccgggcta
     8521 tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat gcaatgccca
     8581 ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg gttcagctat
     8641 tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa gcaataggtt
     8701 ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc ttaaaattat
     8761 ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta tcagaaacag
     8821 tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa ttatatcaac
     8881 gtgatatgaa gaagtacctg ggagagatga agcgtctggc agatggggga gaaatgaatt
     8941 ttgatggcta tctggataag atgtcacatc tggtgaatga aggaacgctc gatcctgata
     9001 ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag tcgtttgacc
     9061 caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt tatgacttgt
     9121 tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa ggccttatat
     9181 aaaaggaccc aatatttatt gggttctttt ttctctatca atgctattag cagggagata
     9241 tatcaccaga gtttaatgtg tgatttttta tttatcgtcg aacatggatt gtttatcatt
     9301 ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag agcttcatgc
     9361 cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg ttatggtata
     9421 gtaccccgct attgagcctc ctgaacagtg aagctgaata acataacccc atgatatatc
     9481 gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa aaatcaacca
     9541 aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta gcgttgcacc
     9601 aatgctcgat aggatgtacc gtttttgaaa aacaactagt tatacacttt gtgggagcct
     9661 attgggaacc tgttattctt agaaagcatc atttgctcat cacttcaatt gaccatggtt
     9721 aaagccagct ataaacttga caaacgaggt aactggttgc ttaggcccaa gtgggcacac
     9781 cataagaagc tcatgttggt gatgaagcaa cccatgaagt atcgagaata attataaagt
     9841 tcactatctt tgaggtttct tatatgtttt gcgaagaaaa agtagctcaa atggctgcat
     9901 acctacttct taagagaggt gggcgcatgg catatctgaa attgatgaag ctgctctatc
     9961 tgtctaaccg ccagtcgatt ttgaagcatg gcaggatgat cggcgaagat agcctttact
    10021 ctatgaaatt tggaccagtc atgtcgaata cgctgaactt gattcgcggt aaggctgaag
    10081 gcattggtga ctactggtac aacttgatag agacgaacgg gcataatgta tcgttgcgtt
    10141 cagatccgag ggaaatggat gcagacgagg tctttgatga attgagtcgt gcagatatcc
    10201 ggattttaga tgaaatctat tctcggtatg ggcatatgaa ccgatttgat ctcgcaaata
    10261 tgacgcattt agaaagcgtt tgtccagagt ggcacgatcc tggcaattct cgtaagccta
    10321 tagacctgaa agaaatgctg atcagtgagg gtaaaagcga ggatgaggct aatcgcataa
    10381 ttggcaaaat ggaagaatct cagaaactta aggaattttc tttgcaatta tcatgacgga
    10441 ttatcagcca tacaggaaag gaactgtgct tgccccaact ggaccatgca atcatcttca
    10501 tgtgatttgt aatgatcctg tttattaccc cgttaacgat tgttattgtg ttttagttgt
    10561 taatatttct agtatcaagg atggtgtccc ccacgatccg tcttgcgtct tgaattctgg
    10621 tgatcatcgc tttatcaagc atccaagtta tgttgtttac gctgaagcta taatttggcg
    10681 agtggataac atggttagaa agcagcgatc gggtgagatt tctgttcatg atgatatgcc
    10741 agaagctaca ttcaatagaa ttctggacgg ttttgatatc tct
//
LOCUS       DADKXM010000131        10666 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.132, whole genome shotgun sequence.
ACCESSION   DADKXM010000131 DADKXM010000000
VERSION     DADKXM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.132"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            383..1501
                     /locus_tag="JQR05_003262"
     CDS             383..1501
                     /locus_tag="JQR05_003262"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY5325341.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWNIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIVLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="JQR05_003263"
     CDS             1567..1815
                     /locus_tag="JQR05_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY5325342.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="JQR05_003264"
     CDS             1880..2248
                     /locus_tag="JQR05_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY5325343.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDQQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="JQR05_003265"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="JQR05_003265"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY5325344.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /locus_tag="JQR05_003266"
     CDS             3103..4350
                     /locus_tag="JQR05_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY5325345.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /locus_tag="JQR05_003267"
     CDS             complement(4418..5794)
                     /locus_tag="JQR05_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY5325346.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JQR05_003268"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JQR05_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY5325347.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..10273)
                     /gene="cusB"
                     /locus_tag="JQR05_003269"
     CDS             complement(9050..10273)
                     /gene="cusB"
                     /locus_tag="JQR05_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY5325348.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFEQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPNKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10289..10621)
                     /gene="cusF"
                     /locus_tag="JQR05_003270"
     CDS             complement(10289..10621)
                     /gene="cusF"
                     /locus_tag="JQR05_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY5325349.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2579 a         2948 c         2597 g         2542 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc acccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgcct cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactggaat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg tgctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat caacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattat cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcagcg agactgatgc catactgcgc caggcgctga
     8461 gggtcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttattcgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attttcgcga ccacgttatc tttggcgata ttcattcccg
     9601 cgcgcagatc aaacgcggtg atcacgccat caatgggcgc tttgagcgta aagcgagtct
     9661 ggattttttg cgtggcgatc aggtggcgaa tatccgcctc cggcattccc gccagtcgca
     9721 gccgctcaag aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat
     9781 actcactctg cgcttccacc cagtcgggaa tggtcaggtc gagaagcggt gtgccctttt
     9841 gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct
     9901 gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag
     9961 tcagcggccc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt
    10021 caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc agatccatat
    10081 ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatc gggtcgtacc
    10141 agaataagat tttacgttct gcggtcggca ttttttctgc gtgcggttcc gcctgttcaa
    10201 accaggtaaa acctgccgct gaaataatac cgcccgcgat catgctgccg ataataagcg
    10261 cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag
    10321 aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta atttcactca
    10381 ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttg acggcagcaa
    10441 tcggatcgtg atggatggtg atttttttgc tttccagatc aacacctttt accacaccgg
    10501 tggcactaat gacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct
    10561 gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca
    10621 tttattctct cctggagtta aaaattattg ctgccaaccg ccccct
//
LOCUS       DADKXM010000132        10494 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.133, whole genome shotgun sequence.
ACCESSION   DADKXM010000132 DADKXM010000000
VERSION     DADKXM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.133"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JQR05_003271"
     CDS             49..579
                     /locus_tag="JQR05_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5325350.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JQR05_003272"
     CDS             597..1235
                     /locus_tag="JQR05_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5325351.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JQR05_003273"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JQR05_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY5325352.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AAHWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JQR05_003274"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JQR05_003274"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY5325353.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JQR05_003275"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JQR05_003275"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY5325354.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JQR05_003276"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JQR05_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY5325355.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VTCATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JQR05_003277"
     CDS             complement(5552..6613)
                     /locus_tag="JQR05_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY5325356.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JQR05_003278"
     CDS             complement(6610..8007)
                     /locus_tag="JQR05_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY5325357.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JQR05_003279"
     CDS             8163..9161
                     /locus_tag="JQR05_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY5325358.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JQR05_003280"
     CDS             complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JQR05_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY5325359.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10222..>10494)
                     /locus_tag="JQR05_003281"
     CDS             complement(10222..>10494)
                     /locus_tag="JQR05_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HAY5325360.1"
                     /translation="TQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFML
                     YTTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT         2640 a         2809 c         2488 g         2557 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgcac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaagtc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagcgtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccattac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtag tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc acgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt attatcctgg
    10201 gggaatgcct tccccctgta tttagcgaat tgctatttcc gtttctgcat caaagaaatg
    10261 acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat catttaacgc
    10321 accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg cgtccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgt
//
LOCUS       DADKXM010000133        10412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.134, whole genome shotgun sequence.
ACCESSION   DADKXM010000133 DADKXM010000000
VERSION     DADKXM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.134"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1723
                     /gene="traD"
                     /locus_tag="JQR05_003282"
     CDS             <1..1723
                     /gene="traD"
                     /locus_tag="JQR05_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAY5325361.1"
                     /translation="QLTDNPKDVARMLKKDGRDSDIRIGDLPIIRDSEIQNFCLHGTV
                     GAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKSLNPLDARCAAWDLWK
                     ECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLS
                     IKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRD
                     WMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDD
                     LPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRS
                     PSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPD
                     LTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMA
                     SLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPVSPVINDKKSDSGVNVPAGGIEQELKM
                     KPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(1732..2130)
                     /gene="vapC"
                     /locus_tag="JQR05_003283"
     CDS             complement(1732..2130)
                     /gene="vapC"
                     /locus_tag="JQR05_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY5325362.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(2130..2357)
                     /gene="vapB"
                     /locus_tag="JQR05_003284"
     CDS             complement(2130..2357)
                     /gene="vapB"
                     /locus_tag="JQR05_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY5325363.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            2511..2858
                     /locus_tag="JQR05_003285"
     CDS             2511..2858
                     /locus_tag="JQR05_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HAY5325364.1"
                     /translation="MLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSR
                     MQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRHHPCGGTGLHT
                     GDDGRTVRNGADR"
     gene            2845..7716
                     /gene="traI"
                     /locus_tag="JQR05_003286"
     CDS             2845..7716
                     /gene="traI"
                     /locus_tag="JQR05_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY5325365.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTSSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQVIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTTDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSTGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            7736..8482
                     /gene="traX"
                     /locus_tag="JQR05_003287"
     CDS             7736..8482
                     /gene="traX"
                     /locus_tag="JQR05_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY5325366.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            8537..9097
                     /locus_tag="JQR05_003288"
     CDS             8537..9097
                     /locus_tag="JQR05_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY5325367.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            9228..9440
                     /locus_tag="JQR05_003289"
     CDS             9228..9440
                     /locus_tag="JQR05_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY5325368.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            9624..10145
                     /locus_tag="JQR05_003290"
     CDS             9624..10145
                     /locus_tag="JQR05_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY5325369.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQ
                     RDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSD
                     ADPVPPWIWRHRK"
     gene            10191..10400
                     /gene="hha"
                     /locus_tag="JQR05_003291"
     CDS             10191..10400
                     /gene="hha"
                     /locus_tag="JQR05_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY5325370.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
BASE COUNT         2460 a         2606 c         3227 g         2119 t
ORIGIN      
        1 tcaactgaca gacaatccga aagacgttgc ccggatgctg aaaaaagacg gcagggactc
       61 cgatatccgg attggcgatc tgccgattat ccgggattcc gaaatccaga acttctgtct
      121 gcacggcacg gtcggggccg gtaagtcgga ggttatccgt cgtctggcca actacgcccg
      181 tcagcgtggt gatatggtgg tgatttatga ccgttcaggg gaatttgtta aaagttacta
      241 tgatccctcc atcgataaga gcctgaatcc gctggatgca cgctgtgccg cctgggattt
      301 gtggaaggag tgcctgacac agccggattt tgataatacc gcaaatactc tgatcccgat
      361 gggcacaaaa gaggacccgt tctggcaggg ttcaggacgt accatttttg cggaagcggc
      421 gtatctgatg cgtaatgacc ccaaccgcag ctacagcaaa ctggtggaca cactgctttc
      481 catcaaaatt gaaaaactgc gtacctacct gcgtaattca ccggcggcca acctggtgga
      541 agagaaaatc gagaaaacgg ccatctccat ccgtgctgtg ctgaccaact atgtgaaagc
      601 catccgttac ctgcagggga ttgagcataa cggtgagccc ttcaccatcc gtgactggat
      661 gcgtggtgtc cgggaggatc agaaaaacgg ctggctgttt atctcgtcga atgccgacac
      721 tcatgcctcc ctgaagccgg tgatctccat gtggctgtcc attgccattc gtggtctgct
      781 ggcaatggga gaaaaccgta atcgtcgtgt gtggttcttc tgtgatgatt tacccacatt
      841 acacaaactg ccggacctgg tggagatcct gccggaagcc cgtaagttcg gtggctgtta
      901 tgtgtttggt atccagtcct atgcccagct ggaagatatc tacggtgaga aagcggctgc
      961 cacgctgttt gacgtcatga acacccgtgc ctttttccgt tctcccagcc ataagattgc
     1021 ggagttcgct gccggtgaaa ttggtgagaa agagcacctg aaagccagcg aacagtattc
     1081 ctacggtgct gatccggtac gtgacggggt atcgaccggt aaggatatgg agcgccagac
     1141 gctggtcagt tattccgaca ttcagtccct gccggatctg acctgctatg tcaccctgcc
     1201 cggaccgtat ccggcagtca aactctcact gaaatatcag gaacgtccga aggtcgctcc
     1261 ggagtttatt ccgcgtgaca tcaacccgga aatggagaac cgtctgagtg ccgtacttgc
     1321 cgcaagggaa gcagaaggtc gtcagatggc cagcctcttc gaaccggatg tcccggaggt
     1381 tgtttccgga gaagacgtga ctcaggctga acagccgcaa cagccgcaac agccgcaaca
     1441 gccggtgtct cctgtcatca acgataagaa gtcagattca ggtgtgaatg ttccggcagg
     1501 ggggatcgag caggagctga aaatgaaacc ggaagaagag atggaacagc aactgccacc
     1561 cgggatcagt gaatccggtg aagtggtgga tatggccgct tatgaggcat ggcagcagga
     1621 aaatcatccg gacacctggc agcagatgca gcgtcgtgaa gaggtgaaca tcaatgtgca
     1681 ccgggaacgc ggggaggatg ttgagccggg agatgatttc tgatgaacag gtcagctcca
     1741 gtcttcagtt ctcaggccgc ccacacgttc aaattcccgg gtgttattag tcacaataat
     1801 cagcccccga ctgcgggcat gacctgcgat catttgatca aatggcccga cagggcgtcc
     1861 ttgacgggca agttctgctc ttatctggcc ggtgtgtgtg gctgcagcag cgtcgtaatc
     1921 cagaacatca atgcgggaaa caaatccctc gatcacagcg agattacgtt caggcatctg
     1981 gcttttttct gcaccatata tcagctccat cagggtgacc gaactgatac acattctccc
     2041 ctggttcagg ttaaaacgct ccctgacgct ggcgggtttg ttctttatcg taaaaatgca
     2101 gatgttggta tcgagcataa acttcagcat cagaatgact ccctttcctg catgccgggc
     2161 tgttccctgt tatccataaa atcggcgctg acgctgtgtc cgtcgaacca ttcgtcccat
     2221 gtctctccgg ctggcgtaat gattctggtt cgtccgacag caatcacttc aacgcgtttt
     2281 acgttttccg gcaatgcaac cgcttttggc agtctgaccg cctggctgcg gttgctgaga
     2341 aatacggtgg tttccattat ttgcctcctt atgtggatat gtcttgtgta tatctattgt
     2401 gggattgata ccgggatatg tcaaagggat atacgtttat gatgagtatc gcgcaggtca
     2461 gatcggccgg aagtgccggt aattattata ccgacaagga taattactat gtgctgggca
     2521 gcatgggaga acgctgggcc ggccaggggg ctgaacaact ggggctgcag ggcagtgtcg
     2581 ataaggatgt ctttacccgc cttctggagg gcaggctgcc ggacggggcg gatttaagcc
     2641 gcatgcagga tggcagtaac aggcatcgtc ccggctacga cctgaccttc tccgctccca
     2701 aaagtgtctc catgatggcc atgctcgggg gggacaaacg tcttattgaa gcacataacc
     2761 aggccgtgga ttttgctgtc cgtcatcacc cgtgtggagg cactggcctc cacacgggtg
     2821 atgacggacg gacagtcaga aacggtgctg accggtaatc tggtgatggc actgtttaac
     2881 cacgacacca gtcgcgatca ggaaccacag ttacacacgc atgcggtggt gactaatgtt
     2941 acgcagcata acggcgaatg gaagacactg agcagtgaca aagtggggaa aacggggttc
     3001 attgagaatg tgtacgctaa tcagattgcc tttggcaggc tctaccggga aaaactgaaa
     3061 gagcaggttg aggcgctggg ctatgaaact gaagtggtcg gtaaacacgg tatgtgggaa
     3121 atgccgggtg taccggtgga ggccttttcc ggacgctcac agactatccg ggaggccgtc
     3181 ggggaagacg cctcgctgaa atcccgggat gtggcggcgc tggatacgcg taaatccaaa
     3241 cagcacgtcg atcctgaagt cagaatggcc gagtggatgc agacgctgaa ggaaaccggg
     3301 ttcgacatcc gggcatatcg tgacgcagcg gaacaacggg catatacccg cacgcagaca
     3361 cccggacccg cttcacagga cgggccggat gtgcagcagg cggtgacaca ggccattgcc
     3421 ggattaagtg aacgcaaagt gcagtttatg tatacggacc tgctggccag gacggtcggc
     3481 atactgccgc cggaaaatgg tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc
     3541 agccgtgagc agcttatccc cctcgatcgt gagaaggggc tgttcacgtc cgggattcat
     3601 atgctcgatg agctgtcagt ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg
     3661 accgtacatc cggagaaaag tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt
     3721 ctggcacagg accgtccgtc gctggccatt gtgtccgggc agggcggtgc agccgggcag
     3781 cgtgagcggg tggctgaact ggtcatgatg gcccgggagc aggggcggga ggtgcagatt
     3841 atcgctgctg accgtcgctc gcagatgaac ctgaagcagg atgaacggct gtccggtgaa
     3901 ctgataaccg gacgtcgtca gttgctggaa ggcatggcct tcacgccggg cagtaccgtt
     3961 atcgtcgacc agggcgaaaa actctccctg aaagagacgt taaccctgct ggatggtgcc
     4021 gcacgtcata acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaagcagt
     4081 gcactgatgg ccatgaagga tgccggggtg aacacatatc gctggcaggg gggagaacag
     4141 cgaccggcca ccatcatcag tgaaccggac cgtaatgtcc gctatgcccg gctggccgga
     4201 gattttgcgg ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggagtacgg
     4261 gaacaggcca tactgacaca ggtcattcgc agtgagctga aaacacaggg cgtgctcgga
     4321 cacccggagg taaccatgac cgccctgtca ccggtctggc tggacagccg gagccgttat
     4381 ctgcgggata tgtaccgccc gggaatggtg atggagcagt ggaacccgga gacacgcagt
     4441 catgaccgct atgtgacaga acgtgtgacg gcgcagagtc acagcctgac cctgcggaat
     4501 gcgcagggcg aaacgcaggt ggtgcgtatt tcttccctgg acagcagctg gtcgctgttc
     4561 cggccggaaa aaatgccggt ggcagacggt gagcgtctga gggtgacagg gaaaattccc
     4621 ggactccgcg tctccggcgg tgaccgcctg caggtggcat cagtcagtga agatgcgatg
     4681 acggttgttg tgccgggacg ggctgaaccg gccaccctgc ctgtgagcga ttcaccgttc
     4741 acggcactga agctggagaa cggctgggtg gaaacgcccg ggcattccgt cagcgacagt
     4801 gcgacggttt ttgcctccgt cacacagatg gcaatggaca atgccaccct gaacggtctg
     4861 gcccgcagtg gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa
     4921 aaacttgccc gccatccctc ctttacggtg gtttctgagc agataaaggc gcgtgccggt
     4981 gagacattgc tggaaaccgc tatcagtctg cagaaagccg ggcttcacac gccggcacag
     5041 caggccattc atctggccct tcctgtgctg gaaagtaaaa acctggcctt cagcatggtg
     5101 gacctgctga cagaggcgaa gtcgtttgct gcagaaggaa ccggttttgc tgacctggga
     5161 ggggaaatca atgcgcagat aaaacggggt gatttactgt atgtggatgt ggcaaaaggc
     5221 tatggcacag gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc
     5281 catattctcg aaggtaagga ggcggtcacg ccgctgatgg agagagtacc tggcgaactc
     5341 atggagaaac tgacatcagg acagcgcgcc gccacccgta tgatactgga aacgtccgac
     5401 cgtttcacgg tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg
     5461 atgtcagccg tgaacatgct gccggagagt gaacgtcccc gtgtcgtggg gctggggccc
     5521 acacaccgtg cggtcgggga gatgcgcagc gccggcgtgg atgcacagac actggcgtcc
     5581 tttctgcatg acacgcagct gcagcagcgc agcggagaaa cgccggattt cagcaacacg
     5641 ctgttccttc tcgatgagag ctctatggtg ggcaataccg acatggcacg ggcatacgcc
     5701 ctgattgcgg ccggtggcgg tcgggctgtg gccagcggtg acacggacca gctgcaggcc
     5761 atcgcgcccg gtcagccttt ccgtctccag cagacgcgca gtgctgccga tgtggtcatc
     5821 atgaaggaga ttgtgcgtca gacgccggaa ctgcgggagg cggtatacag cctgattaac
     5881 cgggatgtgg aaagggcgct gtccgggctt gagcgtgtga aaccgtctca ggtgccacgt
     5941 ctggagggcg catgggcacc ggagcactcc gtgacggagt tcagtcacag ccaggaagcg
     6001 aaactggcag aagcgcagca gaaggcgatg ctgaaaggcg aggcttttcc ggatgtcccc
     6061 atgacactgt atgaagccat tgtccgcgac tataccggca ggacaccgga agcacgggag
     6121 cagacgctga ttgtcacgca cctgaatgag gaccggcgcg tactgaacag catgattcat
     6181 gatgtacggg aaaaggccgg agaactgggg caggtgcagg tcatggtgcc tgtcctgaac
     6241 acagcgaata tacgcgacgg ggagctgcgt cgtctctcca cctgggagaa taacccggat
     6301 gcccttgccc tggtggataa tgtgtatcac cggattgccg gtatcagcaa ggatgacggg
     6361 ctgataaccc tgcaggatgc ggaaggtaac acgcggctga tttcgccccg ggaggcggtg
     6421 gctgaaggcg tcacgctgta caccacggac accatccggg tggggaccgg tgacaggata
     6481 cgcttcacga agagtgaccg ggagcgcggt tatgtggcca acagcgtctg gacggtgaca
     6541 gcggtttccg gtgacagtgt cacactttct gacggtcagc agacccgtgt gattcgcccc
     6601 ggtcaggagc gggcagagca acatattgac ctggcctatg ccatcaccgc ccacggtgca
     6661 cagggggcga gtgaaacctt tgccatcgcg cttgaaggca cggaaggtaa ccggaaactg
     6721 atggccggct ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg
     6781 tacaccgata accgtcaggg ctggacggat gccatcaaca atgccgtaca gaaaggaact
     6841 gcccacgatg tgtttgagcc gaaaccggac cgggaggtca tgaatgcaga gcggctgttc
     6901 agtacggcgc gggagctgcg ggacgtggcg gcaggccgtg ccgttctccg tcaggcgggg
     6961 ctggccgggg gagacagtcc tgcacggttt attgctccgg gacgtaaata tccgcaaccg
     7021 tatgtggcac tgccggcgtt tgaccgtaac ggcaagtcca ccggtatctg gctgaacccg
     7081 ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg ggtaaaaggc
     7141 agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct
     7201 gataatatgc aggatggcgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta
     7261 agaatcgccg gtgaaggccg tccgtggaat cccggtgcca tcaccggtgg ccgcgtgtgg
     7321 ggggatatcc cggacaacag cgtccagccg ggagccggaa atggcgaacc ggtcacggca
     7381 gaggtactgg cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgtcgcgca
     7441 gatgaaattg tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag
     7501 caggctgtca gggaaattgc cgggcaggag cgtgaccggg ctgccataac tgaacgggaa
     7561 gccgcgctgc cggagagtgt actgcgtgaa ccacaacggg tgcgggaagc ggtccgggaa
     7621 gttgcccggg aaaatttgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt
     7681 gacctgcaga aagagaaaac cccgggcgga gactgataca ggaagacaaa cactgatgac
     7741 aacggataac acgaacacga cacgtaacga ttcactggct gcacggaccg atacctggtt
     7801 gcagtcattt ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt
     7861 gctgatggta ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct
     7921 ggccgggcga ggagcctttc cgctttttgc cttggtgtgg gggctgaatc tgtcccgtca
     7981 tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga tggggaatca ttgcccagtt
     8041 cgcatattat ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt
     8101 ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat
     8161 actcctgatg gccctgtggg ggcctttgtc cggcaccagt tacggtattg ccgggttgct
     8221 gatgctggca gtcagcaacc ggctgtaccg ggcggaagac agagcggaac gtctggcgct
     8281 ggtcgcctgc ctgctggctg ttattccggc gcttaacctt gccaccagtg atgcagcggc
     8341 ggtggccggt cttgtgatga cggtgctgac cgttggtctg gtgttgtgtg ccggaaaatc
     8401 attaccccgt ttctggcccg gagacttttt cccgacgttc tatgcctgtc atctggctgt
     8461 gctgggcgtt ctggcgctgt gacgggtgtg gtatctttgg ccgcaagagg atgattcgtc
     8521 agaggcagaa cacagcatga cagagcagaa gcgaccggta ctgacactga agcggaagac
     8581 agagggaaca gcgcctgtcc gcagccggaa aaccatcatc aatgtcacca cgccaccaaa
     8641 gtggaaggtg aaaaagcaga agctggccga gaaagcggcc cgtgaagcag agctggcggc
     8701 aaaaaaagcg caggccagac aggcgctgtc catttatctg aacctgccca cgctggatga
     8761 ggctgtgaat accctgaagc cctggtggcc gggattattt gacggtgaca cgccccggct
     8821 tctggcctgc ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct
     8881 ctcgcataaa aaactgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg
     8941 tgccatgaaa gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc
     9001 tcaggaggag gaagcgtatg cgggagcgcg tctggcgaaa attcgtcgcc agaaccggat
     9061 aaaggcagaa cttcaggccg tgcttgatga gaaataaaaa aagcctcccc cagagagagg
     9121 aggcagggaa ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac
     9181 tgaattattt atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca
     9241 gaaacagttc acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta
     9301 ttctggaagt cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg
     9361 ttgcgcctag gacagactgg cagcagtttg cccggaaaag ggctgaacac tgccaccggc
     9421 gctgccgggg aagggtgtaa cgaaaaaagc agccttcatt actccaggag tcagaaggct
     9481 gaaaggagtt tatgagattt gcttcttaat atccggtcgg gcagtatgac cggacagttc
     9541 tgagtagcac agagtctttt ccgcattaag tcgatttttt atattttgtg attaatttca
     9601 caaaataagg tgttattcaa tgtgtgctgc actattcagg gggtctgaaa tacaggtgcc
     9661 atttatctga tatggagaat aacatgagga aatatattcc actggtatta tttatctttt
     9721 catggccggt attgtctgca gatattcatg gacgggttgt tcgtgtactg gacggtgata
     9781 ccatcgaggt catggactca ctgaaggcgg tgcggatcag actggtaaat attgatgcac
     9841 cggaaaagaa acaggattat ggccggtggt caacagacat gatgaaatca ctggtcgcag
     9901 gaaaaacggt taccgtcaca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
     9961 tttatgcacc agacggaatg aatattaatc agtttatggt ccgggccggg gctgcctggg
    10021 tatatgagca gtacaacaca gatcccgtgc tacctgtcct tcagaacgaa gccagacaac
    10081 aaaaacgtgg gctctggtca gatgctgatc cggttccccc ctggatatgg aggcaccgta
    10141 aataaagcga tggcaatcct atcattcatc tgttctcagc gggaataaaa atggcaaaaa
    10201 caaaacagga atggctgtat cagttacggc gatgttcatc agtcaatacg ctggaaaaaa
    10261 ttattcataa aaacagagac agtttatcaa acagtgaacg tgaatcattt aattcagcag
    10321 cagaccaccg gctagcggaa ttaatcaccg gaaagttata tgacaggata ccaaaagaga
    10381 tatggaaata tgtaaggtaa taattcagca at
//
LOCUS       DADKXM010000134        10405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.135, whole genome shotgun sequence.
ACCESSION   DADKXM010000134 DADKXM010000000
VERSION     DADKXM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.135"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(38..364)
                     /locus_tag="JQR05_003292"
                     /pseudo
     CDS             complement(38..364)
                     /locus_tag="JQR05_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(374..922)
                     /locus_tag="JQR05_003293"
     CDS             complement(374..922)
                     /locus_tag="JQR05_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY5325371.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(922..1629)
                     /locus_tag="JQR05_003294"
     CDS             complement(922..1629)
                     /locus_tag="JQR05_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY5325372.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAISAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIANGLGQLPATIVYSWAGSMLTGCTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JQR05_003295"
     CDS             complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JQR05_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY5325373.1"
                     /translation="MSQHYSVSWEKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2326..3036)
                     /locus_tag="JQR05_003296"
     CDS             complement(2326..3036)
                     /locus_tag="JQR05_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY5325374.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JQR05_003297"
     CDS             complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JQR05_003297"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY5325375.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4455..5675
                     /gene="astC"
                     /locus_tag="JQR05_003298"
                     /pseudo
     CDS             4455..5675
                     /gene="astC"
                     /locus_tag="JQR05_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5672..6706
                     /gene="astA"
                     /locus_tag="JQR05_003299"
     CDS             5672..6706
                     /gene="astA"
                     /locus_tag="JQR05_003299"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY5325376.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTATARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6703..8181
                     /gene="astD"
                     /locus_tag="JQR05_003300"
     CDS             6703..8181
                     /gene="astD"
                     /locus_tag="JQR05_003300"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY5325377.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            8178..9521
                     /gene="astB"
                     /locus_tag="JQR05_003301"
     CDS             8178..9521
                     /gene="astB"
                     /locus_tag="JQR05_003301"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY5325378.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            9514..>10405
                     /gene="astE"
                     /locus_tag="JQR05_003302"
     CDS             9514..>10405
                     /gene="astE"
                     /locus_tag="JQR05_003302"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY5325379.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHD"
BASE COUNT         2394 a         2656 c         2917 g         2438 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattactc ttttaaggtg cggaagtttt
       61 cgccgttcac ccagagcaga tccaccgagc cgcccgtttt acgaccggca gcagcttcgg
      121 tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag
      181 cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc
      241 cgccccaggc gttaaaccag acggtttgcc ctttggcctc atttttaatt tcctgccagt
      301 cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc
      361 gcatcttccc cccttaagaa cgtttttccg gaatacggca cagcccctga gcgggaatgt
      421 ccatggcaga attaaaacga gccgacaggt tttgcaggcc gatcaatgcc gtcagttcgg
      481 tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg cagggcatca tcgactgttg
      541 gcggtgtgac gcttgcggct tcggcgtact ccagcgccag ccgttcttca tcgctaaaga
      601 gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg
      661 cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg
      721 cgctgacaag cgatcgcact accggatcga gcggtgagcg tttgcgctcc agccacccaa
      781 caaacattga caccaaataa aagataaacg ggatccgccc ccaccagcga atcgggctta
      841 gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt
      901 tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg cgctcaagcc atattttctt
      961 cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa
     1021 agtacagcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc
     1081 aagcccgttg gcgataaaaa acgagcgaaa acgtattgaa gtcagaccgg cagcatagct
     1141 gattggatcg aaagggacaa aaggcaataa ccgacagacc aggatggtgt gtttgccgta
     1201 gcgagtgaaa aagccgtcca tactgtcaag cacggttttg ccggttaatt tttccaccac
     1261 ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc gcgccggcca tcgaactggt
     1321 ccacgacagc aagccgcccc agaacgcgcc aaacagcgac gcattggcaa aggtgatcaa
     1381 aaacgcaggt agcggtgcag aaatggcctg taaaatcatc aacaagaacg agacaactgc
     1441 cgccagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa
     1501 cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc atgcgagcag
     1561 catagcgaac aacaggatga tgagggttat acggtagtac cagattttac gcgattgtat
     1621 cttcatcatc ctttatcctg gctttattcg gtgattttaa aggtaaccgt tgccagcgtg
     1681 ttatccgccg ggagaactga ggcattgcct ttgaatttca cttcgccgcg tttttccacc
     1741 gcaccataag tgtatgttgc attgctgacg atgccgaccg ggcagctatc cagacacacc
     1801 aggcaacccg ttttcttctc ttctgctgcc gttaaattac cgccaaagcg catgtccagt
     1861 ttcttgccat tactgtcaac aatcacttca tcgaaggaat acgcttttgc cgccccttgc
     1921 cagttgaccg aaatatccag tttgctgcct gtgacatgag tcgtttcttt attatccatc
     1981 gtcatgtttt cgcccggcgt accacctgcc tctttcaggg cttcataaaa tgctttcggg
     2041 gtcgcataag ccataaacag cgatttatgt ccgttggagc catctttaaa cacaataccg
     2101 tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca ccagcatggt taccgtatgg
     2161 ttattggcat cgacgcgcag cggttggctg ttcgaaagtc cgtcgtattc cacttttgcc
     2221 gccgcattct ctttttgatc gcagccgcta agcccggcaa cagccagtaa gcagagtgca
     2281 gccagtcctt tttcccatga cactgaataa tgttgcgaca tcgttctatc cttcattttt
     2341 tgggtgagta agtgggctgc ggcgcgaagc agacagatcc acatgtttgt gtcgggcgta
     2401 gagttttgcg agctggatga gaataaacag cgccaggccc gccagacaga gttgtaaaat
     2461 aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc atcacggtat aaataacaat
     2521 accgggcagg gtcgtgagtg ccgaaataag ggtataaggc cagaaggcga tggtggttaa
     2581 tccgtaagcg taattttgaa tattgtaagg aaacaacggg attaagcggg tcagaataag
     2641 aaaatcaata ccgttacgcg caatgccttt ttcaatggcc tggaaggtat ggctatggcc
     2701 cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg agcacgagga
     2761 ggccagcgtt gcggcaatta atgagagtag tgtccctaag agcgggccaa aaactattcc
     2821 tccagcgatc accagtatgc ttcctggtaa cagaaagagg gtggcattga tgaataacag
     2881 aatatagagg ctataaccga acaatccgct ctggcgaatg agtgtctgta agtggggtaa
     2941 atcggtgagc agatcgaata aaccgaaagc gtggatagcg taaatgacca gcgcaaaaat
     3001 aagacaggca aacagaaagt tacgctcagc gttcatcgtg gagtttccta tatcaaactt
     3061 gataatagag aagtggtcag acaactaaaa acgcagtgac ggtataacaa aggacggcag
     3121 gcaacaaatc attactgaca gtttgttgat ttttctcaag gttaattctc ctgacccagt
     3181 ttgagccagg agagctgcta aattagcggc ggaaggtcgc ccagacgggg gcgtgatcgg
     3241 acggtttttc catgctgcga atttcatagt cgatgccggt ttctacgcaa cattctgcca
     3301 gcggttggct ggcgagcagc aggtcgatgc gcagaccacg gttatcgtca aaaccttttg
     3361 agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg attagcatgg cggaaggtat
     3421 cgaccacccc ccagctcatc agcctgtcca tccattcgcg ctcttccggc aggaaagagc
     3481 atttaccggt acgcagccag cgcttacggt tttcttcgcc aatgccgata tccagatctg
     3541 tagggctgat attcatatcg cccataatca gtaccggatt atcacgtttg agttcggttt
     3601 ccaggtagtt ttgcagattc tgataaaact gcgcttttgc cgggaatttt atcggatggt
     3661 cgcggctttc accctgcggg aagtaaccgt tgatcacggt gacattaccc agcagtgagg
     3721 ggatttccgc cataataatc cgccgctgcg cctcttcgtc gtcaccggga aagccgcgac
     3781 gcacggcaat cggcgtctct ttggtcagca gcgccacgcc ataatggcct ttctgcccgt
     3841 gataaaacac gttgtagccg agcttcgcca cctcttcgag cggaaacata tcgtcatgaa
     3901 cttttgtctc ctgcaggcca atcacatccg gttggtgctt ttcgacgatg gcttcaagct
     3961 ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac aaatttcata gtcgctgcca
     4021 tttattcaga taaagagtgc gtggatggta gcagaatttc gcgttgctta ccgcctgttg
     4081 ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa atgcccgctt
     4141 ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt aaatttcgtc aatgatcaca
     4201 attccagaat tatattttac ttctgcataa cattgcgttt tttattcttt ttattcaccc
     4261 acatcgcaaa cgtattcact ttatatgcac tttaaatgca tatggtttgg ttataactcc
     4321 ttgatttcca gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg
     4381 caatctacat ttacagcgca aacattactt attgttaaca tacaaataac gaattattta
     4441 ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac
     4501 ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc ttgtgggatc
     4561 agcaggggaa agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg ctgggccatg
     4621 cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg cataccggca
     4681 acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg
     4741 ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg
     4801 cgcgtaaatt cgctcacgac cgctacggca gccataagag tggcatcgtg gcgttcaaaa
     4861 atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca gcctattcac
     4921 aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat attaactctg
     4981 ccagcgcgct gattgacgac gccacctgtg cggtgattgt cgagcccatc tagggcgaag
     5041 gcggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg tgtgaccgcc
     5101 acaatgcctt gctgattttt gatgaggtac aaaccggcgt cgggcgcact ggtgagctgt
     5161 atgcctatat gcactacggc gtgacgcccg atctgttaac caccgccaaa gcgctgggcg
     5221 gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg atgaccgttg
     5281 gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca ggcaaagtgc
     5341 tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac gactggtttg
     5401 ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt cgcggcttag
     5461 gtttgttgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa cagatctctc
     5521 tggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg gtgcgttttg
     5581 cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc tttgcagctg
     5641 cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg ttgagcgctc
     5701 agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgcttcc
     5761 cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct ggcaaggcga
     5821 actgcccaaa agcgagcagg gctatgtgtt cgtgctggaa gatagcgaga caggcaccgt
     5881 ggcggggatt tgcgctattg aggtggcggt tgggctgaac gatccctggt acaactatcg
     5941 cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat tgccgacgct
     6001 gtttcttagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga
     6061 ctggcgtaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc
     6121 ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga ttgacgaaca
     6181 cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg
     6241 cgccgatttt ctctgcggca ctgggcaaaa ggcatttatt gcagaactga tgccgaaaca
     6301 tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc aggtacatcc
     6361 gcaaaccgcg actgcccgcg cggtgctgga gaaagaaggt tttcgttacc gcaactatat
     6421 cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc gcgccatccg
     6481 taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt ttccggcctg
     6541 cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggcgcgta ccgatccggc
     6601 aaccgagcgt ttgattttaa ccgccgcaca actggatgcc ctcaaatgcc acgcagggga
     6661 tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggattaac
     6721 ggtgactgga taacgggcca gggcgcatcg cgtgtgaagc gtaatccggt atcgggcgag
     6781 gtgttatggc aaggcaatga tgccgatgcc gctcaggtcg aacaggcttg tcgggcagcc
     6841 cgtgcggcgt ttccgcgctg ggcgcggctc tcattggctg aacgtcaggt cgttgtcgaa
     6901 cgctttgccg gattgctgga aagcaataaa gccgaattaa ccgcgattat tgccagagaa
     6961 acgggtaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa taaaatcgcg
     7021 atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat gccggacggt
     7081 gcggcgagcc tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc
     7141 cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc
     7201 tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag
     7261 gctggcttgc cgccgggcgt gctgaacctg gtgcagggcg ggcgtgaaac gggtcaggcg
     7321 ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa taccggctac
     7381 cagctgcatc gccagctctc cggtcagccg gagaaaattc tcgcccttga gatgggcggt
     7441 aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca tctgaccatt
     7501 cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt attgctcaaa
     7561 agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca gcgattaacg
     7621 ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc tgaacaggcc
     7681 gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg aaccctgctt
     7741 gcgccgcgct tattacaagc agggacatcg ttgctgacac cagggatcat tgaaatgacg
     7801 ggcgttggcg gtgtgccgga tgaagaggtg ttcgggccgc ttttgcgcgt ctggcgttat
     7861 gatacttttg atgaggcgat tcggatggcg aacaacactc gcttcggact cttttgcggt
     7921 ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg ggcggggatt
     7981 gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg cggcattggt
     8041 gcttccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc atggcctatg
     8101 gcgagcctgg agtcggactc gttaacattg cccgccacgc ttaaccccgg gctggatttt
     8161 tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg gtagggctga
     8221 cgcatcatta cgcgggcctg tcgtttggta atgaagcctc tacccgtcac cgttttcagg
     8281 tgtctaaccc gcgactggcg gcaaaacagg gcttactgaa aatgaaagcc cttgccgatg
     8341 cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc
     8401 agttgggatt cagtggtagc gatgagcagg tactggaaaa agttgcacgc caggcaccgc
     8461 actggctttc cagcgtcagt tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg
     8521 cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata
     8581 aatttcaccg ttcgctggaa gcgcccgtca ctgaatcgct gttaaaagcg atttttaagg
     8641 acgaagagaa atttagcgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg
     8701 gggcggcaaa ccacaatcgt ctcggcggtc attacggtga accgggtatg ctactttttg
     8761 tctacgggcg agaagaaggc aatgataccc ggccttcccg ttatccggcg cgacagactc
     8821 gcgaagccag cgaggcggtg gcaaggctta atcaggtgaa tccccaacag gtgattttcg
     8881 cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga
     8941 gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca cagttactgg
     9001 caaacctgcg ggcgcgggtg aacggtttta tggcgataga agttccggca actcaggttt
     9061 ccgtgtcgga tgcggtgtct acctatctgt ttaacagcca actgttgagc cgcgatgatg
     9121 gttccatgat gttggtgctg cctcaggagt gtcgggaaca cgccggagta tggggttatc
     9181 tcaatgaact ccttgccgct gacaacccga ttagcgaact aaaagtcttt gacttacgtg
     9241 aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta ttgacagaag
     9301 aagaacgccg ggcggtgaat ccggcggtga tgatgaacga taccttgttt aatgcgctca
     9361 atgactgggt ggatcgttac taccgcgatc gtcttactgc tgccgatctg gccgacccgc
     9421 aattgctgcg cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt
     9481 cggtttatcc gttccagcgc gagggagggg gcaatggata attttcttgc tctgacctta
     9541 acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg
     9601 ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcgct ggtgatttct
     9661 gcggggatcc acggtaatga gacggcacct gtggaaatgc tggacgcgtt gcttggcgcg
     9721 atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg gaatcctcct
     9781 gcgctgaagc aagggaaacg ttattgccat agcgatatga accgaatgtt tggcggtcgt
     9841 tggcagctat ttgctgaaag cggagaaacc tgtcgggcgc gcgaactgga acagtgcctg
     9901 gaagattttt atgaccaggg caaagaatct gtgcgttggc accttgatct acataccgca
     9961 attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat tccctgggac
    10021 gagaaatttc tgacgtggct gggtgcggcg gggctggagg cgctggtgtt ccatcaggag
    10081 cctggtggta cgtttaccca tttcagcgtc agacattttg gcgcgctggc ctgtacgttg
    10141 gaacttggca aagcgttgcc ctttgggcaa aacgatcttc gccagtttgc agtaactgcc
    10201 agcgcaattg ctgcgctgct atctggtgag agtgtcggta tcgtgagaac accgccgctc
    10261 cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca
    10321 agtgacacgc tgaattttat gccgtttgag aaaggaacat tgctggcgca ggacggagag
    10381 gaacgtttta ccgtaaccca tgatg
//
LOCUS       DADKXM010000135        10388 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.136, whole genome shotgun sequence.
ACCESSION   DADKXM010000135 DADKXM010000000
VERSION     DADKXM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.136"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JQR05_003303"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JQR05_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY5325380.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JQR05_003304"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JQR05_003304"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY5325381.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRINMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="JQR05_003305"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="JQR05_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY5325382.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGVGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="JQR05_003306"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="JQR05_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY5325383.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="JQR05_003307"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="JQR05_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="JQR05_003308"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="JQR05_003308"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY5325384.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="JQR05_003309"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="JQR05_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY5325385.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNMETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTFQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /locus_tag="JQR05_003310"
     CDS             complement(8007..9434)
                     /locus_tag="JQR05_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY5325386.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWISLTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGIAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="JQR05_003311"
     CDS             complement(9457..10149)
                     /locus_tag="JQR05_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY5325387.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLMAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2542 a         2507 c         2741 g         2598 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctact aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagcc gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcaata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct
     3001 ttaaaagcga caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 caggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgta ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgct gatgaacagc
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt attgatcctt gatgagccta cccgtggagt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatag acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggctgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgttg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataacat
     6361 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ctctttcccc
     6481 atcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcgttat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaag ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc agccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgtttggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaata tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttactgtt actgtgtacc gaaacgcatc agccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacctttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggcata
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa actgcgtcga catagctatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaatgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg gtacacagcc ggtccccaga cgggttgcaa tattgcctac gataccgatc
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt acgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggaaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg aaaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattaattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatacc ggaagaagtt cattacgagg ataagcacgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccttg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caacgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgacat actacgcttc
     9421 tttttatcgc tcattcaatg tcctgttatc gggttatcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg ataatcgcat cgacaatcgc
     9541 ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccattaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccgggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctg
//
LOCUS       DADKXM010000136        10294 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.137, whole genome shotgun sequence.
ACCESSION   DADKXM010000136 DADKXM010000000
VERSION     DADKXM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.137"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..638
                     /locus_tag="JQR05_003312"
     CDS             <1..638
                     /locus_tag="JQR05_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5325388.1"
                     /translation="LVDVFREAIAEGHVATNPVTATRTAKSEVRRSRLTANEYIEIYH
                     AVEPLPIWLRLAMDLAVITGQRVGDLCRMKWSDINDNHLHIEQGKTGAKIAIPLTLTI
                     DALNISLVDTLQKCREASGSETVIASTHHEPLSPKTVSKYFTKARNASGLSFDGDPPT
                     FHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRDSRGREWDKIEIDK"
     gene            complement(696..1256)
                     /locus_tag="JQR05_003313"
     CDS             complement(696..1256)
                     /locus_tag="JQR05_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY5325389.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLHV"
     gene            complement(1253..2941)
                     /locus_tag="JQR05_003314"
     CDS             complement(1253..2941)
                     /locus_tag="JQR05_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY5325390.1"
                     /translation="MSGEKKTKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(3090..5717)
                     /gene="gyrA"
                     /locus_tag="JQR05_003315"
     CDS             complement(3090..5717)
                     /gene="gyrA"
                     /locus_tag="JQR05_003315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY5325391.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALYHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5864..6586
                     /locus_tag="JQR05_003316"
     CDS             5864..6586
                     /locus_tag="JQR05_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY5325392.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(6714..10294)
                     /gene="yfaL"
                     /locus_tag="JQR05_003317"
                     /pseudo
     CDS             complement(6714..10294)
                     /gene="yfaL"
                     /locus_tag="JQR05_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2423 a         2842 c         2505 g         2524 t
ORIGIN      
        1 cccttgttga cgtttttcgt gaagcaatag ccgaggggca tgtggctacg aatccggtaa
       61 cagcaacccg cacagcaaag tcagaagtaa ggcgctcaag gcttacagct aatgagtata
      121 tcgagattta ccatgcagtc gaacctctcc ctatctggct gaggctggca atggatttgg
      181 ccgtcattac agggcagaga gtgggcgatt tatgcagaat gaagtggtca gacataaacg
      241 acaaccatct tcacattgag cagggtaaaa caggagccaa gattgccatt ccgctgacgc
      301 taacgattga cgcgctcaac atctcactgg ttgatacact acagaaatgc agggaggcca
      361 gcggcagtga aactgtaatc gcctcaacgc atcacgaacc gctttccccg aaaacagtat
      421 cgaagtattt tacaaaggcg agaaatgcct ccggactctc atttgatgga gacccgccaa
      481 cattccatga actgcgtagc ctgtcagcga ggctataccg gaaccagatt ggcgataaat
      541 ttgctcaacg tcttctcgga cataaatcag attcaatggc ggcgcggtat agggacagcc
      601 gtggacggga atgggacaaa attgaaatcg acaaatgatt ttattttgac caataatgac
      661 ctgctcatct taacagattg ataattaatg caattttaaa catgcaactt ccatgtcata
      721 agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct tccggtgtgg
      781 tagatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg atcgaaaaaa
      841 atcatatcgc caggcagcgc ctggtttatg tcttggccaa taaactgact gttgtactga
      901 atcaaattaa tcgcggtcac gtaggggccg tttttcccgt tcccctgatt ccagttttgc
      961 gccagttgac gctgttcagg tgttagcgtc atctctggcg gcaaatactg gctagataaa
     1021 ccgttacttt ttagccattt actgtcgtga actttcagcg tctcgttcgc cgcaaatcgc
     1081 accaggcccg cacaatcctg ctgataccag cgtggacttg gcccctggcg gagctgttct
     1141 tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt gttcgacatt cagcatttcg
     1201 ctatgggcaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg tctcatagcg
     1261 gctgccaggt tatgggtagc cactgccagg cgacaccggg ttccatctgg gccagcttca
     1321 tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa gtttgtgcgg
     1381 cgttataaaa aaccggttcg agattcttcg gcagactggt caacgtttcg ttacgcagca
     1441 gtttcgccac gccttgtgga ttgatataga gcggaacgat gccatcagtg ggtattacat
     1501 ccaccattgc cgggcgggtt ttattcagtg tttgcagggc gttattaacc agcgtgtcat
     1561 cgagggagaa aagcagcgtt ttgttttgca tcgccagcga cacacggaaa aaataatccg
     1621 acattaattg atcgggttgc gccgcctgcg ctttcggata ctgaccgtat cgggaactca
     1681 cttcgcgaca ccagatttgc gcttcgccct gctgagtctg gcttaccggc aaaacacctt
     1741 ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccct ggcaattgcg
     1801 cctgttcggc agtgccatca aactgaccga caaacagcgg ggtttgcaat tttgagtctt
     1861 cataccagca cagccccgcg gcaccgtcta acgccccatt cagcttgtcg ttttcctggc
     1921 tgatgtgcga aagcatctct tcggcaatac cgtgtgaata cggcaccgcc acacagaagc
     1981 tggctccggc aggcatactg ttccagaccg gcgtaaaatc gaaactggca tctacgctgg
     2041 cggattcatc atttaacgcc acaaaactgt gccagccgtc gttacccatt tcgaagcgta
     2101 cgccagcaaa agaaggcatt aaccgctggt agccaaaacc cagcaacctg gcgctggcca
     2161 cgatgcgcag gcgtactggc gttttttcgg cagtacgctc ttccaggcca aagctggctt
     2221 gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc cgtggcttcg gtatcctgct
     2281 gatcatcttt aaacaacata tccgtactgg aaaacaccag catcttgtcc tgatatgtcg
     2341 cgaacatcag ggcgttattg ccgttatagc gcaactgata aacaggaata gtttcactat
     2401 ttatcttgat gctactgatt tccgttttgc ttaactggct gtcgctggta gcggcaaaca
     2461 acaatggctc cagcagtttg cttaaaccac tgcgctggat cagcaccatg taatgtgaaa
     2521 gatggccttg tttatcgtgc catagcgccg cctgcgcggg ctgatctaag agtgacgaaa
     2581 agagcttatc tttcagcgtg agatcgtgtt catagacaat gcggcgaatg ctgccttcaa
     2641 tgcccagacg atcggcatga ttctgataat agaaaacgaa atcttcgctc agaacatcgt
     2701 ggagaaacgg aatggtgagg agatctttgg gaagctggct cagagagtcg ctgtctagaa
     2761 agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc ggcgacaacg
     2821 ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa agcagtgcta
     2881 ttgcgccaaa acctacaaga ccatagaacc gccagccttt cgtctttttt tcaccactca
     2941 ttgccacatt ccttgtgtat agccagccat tttttacggg cacagccaaa ctttaccgcg
     3001 ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa acaagggaga
     3061 tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc gtcaacgtcc
     3121 acttccggag cgatttcatc atccccttcc gcggcactgc cgtcgatggt atccagatct
     3181 tcctcgtcaa ccggttcagc aacacgctgc agacccacta cgttttcatc ttccgcagta
     3241 cggatgagga tcacgccctg ggtgttacgg cccacgatgc tgatttctga aacgcgagta
     3301 cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc tacctgcacc
     3361 gcgccaacca ctaaaccgtt acgttcggta accttgatgg agataacccc tttcgtcgca
     3421 cgcgacttgg ttgggtattc cgccactgca gtacgtttac cgtaaccgtt ttgcgttgcg
     3481 gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc gccttcacct
     3541 aagcgaatac cgcgcacacc agtggtattg cagcccatcg cacggacaga agactcttta
     3601 aagcgcacca ctttaccttc ggcggagaac agcattactt catcttcgcc actggtcaga
     3661 tcaacgccga tcagctcatc gccgtcaacc agtttgatcg ccactttacc ggcggtacgc
     3721 agacggttga attcggtgag gacggttttc ttcacagtac cgttagcggt cgccatgaag
     3781 actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg cggtgatacg ttcgtcctgt
     3841 tccagcggta gcaggttgac gatcggacga ccgcgcgcgc cacgagtggc ttccggcaac
     3901 tggtaaactt tcatcgaata gacgcgacca cggctggaga agcacagaat atggtcgtgc
     3961 gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc ggcagattta
     4021 cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg gctgatattt aacgtagccc
     4081 tggtgagaga gcgtcacgac cacatcttcc tgggtgatca gatcttccag gttgatgtct
     4141 gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga actgttcacg aaccagctcc
     4201 agctcttcgc ggatcacttc catcagacga tcggcgctac caagaatacg caacagttcc
     4261 gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc aaggccggtc
     4321 agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag gtagtacaga
     4381 ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc gtcgccagca
     4441 cgttccaaca tggcggcaac gttgcccagc tgccacggat tagcaaccag cgcagttttc
     4501 gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcaat gttcgccagc
     4561 gccacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag ttcgaaaata
     4621 gtacgacggg tcaccacttc acggcggtga cgaacaaacg ccgcgatgat gtctttcagg
     4681 ttcatgatct tcggctgacc atggtacaat gccaccatgt tgataccgaa agaaacctgc
     4741 aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc gcgtttcact
     4801 tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct gatgccttcc
     4861 acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt gtttacctga
     4921 tacggaattt cgtgaacgat aatggtttca cgtccggttt tggcgtcaac ttccacttca
     4981 gcacgggcgc ggatatatac cttgccgcga ccggtacggt aagcttcttc aataccgcga
     5041 cgaccgttaa tgattgccgc cgtcgggaag tccggccccg ggatgtgttc catcagccct
     5101 tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac ttccgtcagg
     5161 ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc gttcaccagc
     5221 aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc atagttatca
     5281 acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat tttcgccaga
     5341 cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc gaagttaccc
     5401 tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac gatcgtgtca
     5461 taaaccgccg agtcaccatg gggatggtat ttaccgatta cgtcaccaac gacacgggca
     5521 gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta aagtacgcga
     5581 cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac aatgaccgac
     5641 atcgcataat ccagatagga gctctttagc tcttcctcaa tgttgaccgg tgtaatttct
     5701 ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc aaaggtcgca
     5761 aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac atccaatgcc
     5821 tgatatactc gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc
     5881 gccggaaaac cataacgtag accacgaaga gatcgctaaa tttgaagccg tcgcctcccg
     5941 ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc attaacccgc tgcgtctggg
     6001 ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg tcggttgtgg
     6061 cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg gtctggatat
     6121 gggctttgag ccattgcagg tggcaaagct gcacgcgctg gaaagcggca ttcaggtgga
     6181 ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt atgatgtggt
     6241 gacctgcatg gagatgctgg agcacgtacc cgatccgcag tcagtggtca gagcctgtgc
     6301 gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca acggcaagtc
     6361 atggctgatg gcggtggttg gcgcggaata tattttgcgc atggtgccca aaggtacgca
     6421 tgatgtgaag aagtttatta aaccggcaga gctgcttggc tgggtggatc agaccagttt
     6481 aaaagagcga catataaccg ggctacatta caacccgatc actaattcct ttaagctcgg
     6541 ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt gatgtttggc
     6601 cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag gcctacaaat gctctccgta
     6661 ggccagataa ggcgtttacg ccgcatccgg caattgtgat ggtcagacag cgattaccat
     6721 ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat catcactccc tttctgccac
     6781 gccacgccac gctgccgcgc agggacactc gctgactgat attgcccgtg actcccactt
     6841 ttatttcacc ccgtagcttc accgcatcgt cactgatagt gctgccatct tcttcaattt
     6901 ccgtcgcatg gggatcgtga taataattca gatctaatgt tggtgtgaca ccaaccgcgg
     6961 tacgccattc gctgtgtaaa cccagccgcg tctgaatatc atccccctgc gattgtgaca
     7021 cgcgcgcatg gttagcggcg gtaaaatcat cctgctgcac gccctgataa atcacctgcg
     7081 cctgcggttc aatcaccaca ccacgccccg gtaaccactg atacccggct tccagcgagg
     7141 cgataatccc cgacgaatgg taacgatcca cgccatcttc atgttcggaa acatcattgt
     7201 taaaccacgc gtattgcagc cagctatcca gccaggcccc ctgcttctga ttaccgtgct
     7261 gataccagct tgaggtcagc ccaacggcat aaccgtggtt ctggttatcg gcgcgagttc
     7321 cggtcatatt cgagcggctg tcgccctgat tatcactgta gccaccaacc gccccaagca
     7381 tccactcgcc atccgtgccc cagcgcccgc taaacaagcc gccgctaagc tgcaccgtag
     7441 aagtgtcttc ctgctgagcc agttgccctg ccgccgtgta atgataacgt ccgccgataa
     7501 cacgtaaatt cagcgtctga ccatcgccac cagcgtgatc gcgtcgctcc atcataaacg
     7561 cctaatttgc cgcccgcagg ttattgagat aaccgccaac tttggcattc agcaccggct
     7621 ggtaagcagg cgtaggggcc gggtcggggg ttgggtcagg atccggcgtg ggatcaggat
     7681 cgggagtcgg gtctggctca ggtggcgatg gcggcgttac ttcttgcgat cgcagatacc
     7741 agtcgttgtt atcttccact agcgtgtagt catacgctcc catgttgacg tagccactgc
     7801 cagccagact gaactgtgca ttgttttgaa actgcgtggg atcagctgcg aaatcaacca
     7861 ctttaatgcc tgtcgatgtc ggctcaccaa tccctgtaat gaagttaacc accacagttg
     7921 tgttgccagc agtattaccg ttcatcacca attgatcgct taccgagtca tcgccgttta
     7981 attcgctgtc gaatagtagc gtaccgccgc cggtataatc accgtttacc gtcagcgtat
     8041 cgccagcgac accattttgc aggctaaccg caccggacgt attggtcaga tcaccattaa
     8101 ccgtcacgtc agcaagatag gtcaggctgt cagtttcaag gttggtatta agctcgcctg
     8161 tcaacgtcac gccatcggta gcatttacca gcgtcccatc gccgttaaat agtgaggcat
     8221 taagggcgac ggaagtatcc tgcccggtca gacgcaaaac cgtaccatcg ctgatgtcga
     8281 tagtgccgct ggaagtagca tcaattgact gaatatcctg atccgcgcca gtaacgaacg
     8341 ttgccccatc accaacccac agtgacgaag cataaggaaa gatatccgca acatcgcctt
     8401 tcagggtgcc ttcttcgaca cgtaccgccg actgcgttgt accgctggcg gtcagctcca
     8461 gcgtacccgc ccccgtttta gtaaatgtgc taccctcatc ctgatgctgc ccgctgctgt
     8521 cagccatcag tgcgccccac tgcgtgtcta cccccgcatc aactgccact tcaccgtcgg
     8581 cgcgcatttc aatatcacgc ccatgctcag tcgtcgtaga gcgagtatcg acattggcag
     8641 taatctccat cacgctttct tgttgcttat cggtaaagat aacctggcgg ttatagtggg
     8701 tatctccaag ctgcgagttg tcgctcacca tcagcgtacc ggaggcgatt tgtgttgtgc
     8761 ccaaataact attattgttt gccagactta cctgcccgtc gctcgcatcg accaccacgt
     8821 agacgccatc tttcccgtcg ccgccgatat gcaaattgtc gccacctgca agatctacca
     8881 catcctgact gccgataacc gttccgctgc tgccactgac ttcaaggcca tcattgtatg
     8941 atgtgccgct ctgccaggtg gagaaatcag agagatcgag cacaccgccg tttaacacga
     9001 tcgactgcgg atcgtcctgg agagcggtaa gatctgccgc gtcgccttcc agcgaaagca
     9061 ccgcaccatc atcaacgact atatcgccgg ttagcgccat cgactgcgcc cctgccagca
     9121 cgtagctgcc gttttgcgca atggtgagct gaccagcacc ttcgatgatg ccagcaaaac
     9181 tgccctgatt aacagtaacg ttgccaccag catcgatatt taaagtgcca ttctgaaagc
     9241 ccgtcaatgc gtgcacaaaa gtttgttggg tcgagccaac gtttagctca gcctgattct
     9301 gatactgatc aatactccct atcgtcagac cgtagcagtc ttgcggatcg tcctagcaat
     9361 gcgtatcgcc gacattcatc agggagttgc tgcgacccag ggtaacttca ccgttttcaa
     9421 tctgcatctc accagtaaag tcattgttat ctgcattaag taccagatcg ccggaacctg
     9481 ttttggtgat taacccggta ccagcaatag agtcaacagc tccgtcattc tctgtattgc
     9541 cgataaccag cgtttttccg tcggcaatat caaaggtaac ttcacttaag ccgagataca
     9601 taaagccacc cgccgcagtg gaaggaccat ctccatagcc tgctgcgcta ttgttctcat
     9661 cgactaacac gccgccgttc tggctgtagc tgtcatcaac agaaatatca ataagatagg
     9721 gagccgtcgc gctattggta tatatcgccc cgccataacc ttcggctgtg ttatttgtaa
     9781 aggcggtgtt atttattatc gtataacctg aaggatgctt gctgtcgcta ttattatcgg
     9841 taacatcgat tgccccgcca tcgccatcat tgtaacttgt tgatgtatat gcttggttat
     9901 tgttaaaaac atcatcactt aaatagacgt cattattaat ggtataaatt gcgccacctt
     9961 tgccatcatt agcgatatta ttgcgaaaca tggcgttagt gacacgtaaa tcgacggcac
    10021 cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg acgttaccgg
    10081 aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg gccccgccgt
    10141 tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt tcattttctg
    10201 gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg gcattattgg
    10261 tcatatccga aaaaaccagc cattgcccat cggc
//
LOCUS       DADKXM010000137        10240 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.138, whole genome shotgun sequence.
ACCESSION   DADKXM010000137 DADKXM010000000
VERSION     DADKXM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.138"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(46..1983)
                     /gene="betT"
                     /locus_tag="JQR05_003318"
     CDS             complement(46..1983)
                     /gene="betT"
                     /locus_tag="JQR05_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HAY5325393.1"
                     /translation="MLFRDFSALWIGRTLDWVSKTFGWYYLLAATLYIVFVVCIACSR
                     FGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEA
                     ARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYPIFGKRINGPIGH
                     SVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIALSVIIATISVT
                     SGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFMGMTLNSFA
                     FDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFTFTLLWL
                     SVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLAQYPAFTFSASVATITGLLFYVTS
                     ADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVIMG
                     LPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGT
                     RYTKQMMETVCYPAMEEVAQELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFV
                     YQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYER
                     HLNFIHLHREAPGHSVMFPDA"
     gene            2148..2735
                     /gene="betI"
                     /locus_tag="JQR05_003319"
     CDS             2148..2735
                     /gene="betI"
                     /locus_tag="JQR05_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HAY5325394.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2749..4221
                     /gene="betB"
                     /locus_tag="JQR05_003320"
     CDS             2749..4221
                     /gene="betB"
                     /locus_tag="JQR05_003320"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HAY5325395.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNLGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4235..5905
                     /gene="betA"
                     /locus_tag="JQR05_003321"
     CDS             4235..5905
                     /gene="betA"
                     /locus_tag="JQR05_003321"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HAY5325396.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALSRSTAGYFVANGMPVRAKK"
     gene            6119..6795
                     /locus_tag="JQR05_003322"
                     /pseudo
     CDS             6119..6795
                     /locus_tag="JQR05_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7038..7733)
                     /locus_tag="JQR05_003323"
     CDS             complement(7038..7733)
                     /locus_tag="JQR05_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY5325397.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7726..9153)
                     /locus_tag="JQR05_003324"
     CDS             complement(7726..9153)
                     /locus_tag="JQR05_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY5325398.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9164..9883)
                     /locus_tag="JQR05_003325"
     CDS             complement(9164..9883)
                     /locus_tag="JQR05_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY5325399.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCIYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2572 a         2775 c         2547 g         2346 t
ORIGIN      
        1 ttcccgcgcc atatgcagac atggcgcggt tttatgcaaa aacaatcacg cgtccgggaa
       61 catcacgcta tggcccggcg cttcacgatg gagatgaata aagttaaggt gccgctcgta
      121 ctggtcaaga atatcggtga tcacctgctc tttgctgtag tccatcaggt cgttgccctg
      181 gctgccttct aacaggaaag tttccagccg gtagtaagtc gatttaccgc tacgtgcgcg
      241 gtaggtaaag cccggcaccg aatattgctg cggccaaatc tgatagacaa agttttgctc
      301 ttcgcccata tgcaccaaca aatccagatg ccccaactgt tgtccctctt ccggcggcag
      361 gctttttagc tccacgtacg cgccgcgcaa ccgcaactcc tgcgccactt cttccattgc
      421 cgggtaacag accgtctcca tcatctgttt agtgtaacgc gtgcccggat aattcatcag
      481 gcgcgagaga cgttttttcc agctcaggcg atcctgaagc cccagcggtc gcggcgcggt
      541 atcgcggttg gcactttcac ggcggtaatc ttctaccttc agagatttat acaaccccgc
      601 catcacgaag aaaatcacaa agctgaacgg cagccccatg attaccgtgg tgttttgcag
      661 cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc agaccaatcg ccaccgacca
      721 gaagacgcgc agccagccgg gggcgtcgct gttgatatct ttaagctgcg aggtgaaatt
      781 ccccagcacc agcgcaccgg agtccgccga ggtcacataa aacagcaggc cagtaatggt
      841 ggcgacggag gcgctaaagg taaacgccgg atactgcgcc agcaggctgt agaagccgcg
      901 ctccggatgg accatcgctt cctcggcaaa tgccgcgccg ccgtggatga tttcatacag
      961 cgcgctattg ccgaacaccg agagccataa cagcgtgaag gtaaacggaa taatcaacgt
     1021 gcccagcacg aactggcgaa tggtacgccc acgcgagata cgcgccagga acaagccgac
     1081 aaacggcgac catgccaccc accatgccca gaagaagagc gtccagttat tcatccactc
     1141 aaccggacgg tcgaaggcaa aactgttgag cgtcatgccc ataaagcgat tcacatagtc
     1201 gccaacattc agcaccagcg cattaagcag gaatgaggtg tcgcccataa acaatacgaa
     1261 caggatcaat cccagcgcca gcgcaacgtt aagttccgat aacacacgaa tccccttatc
     1321 gacaccggag gtgacagaga tcgtggcgat tatcaccgac aaggcgatca gtgccgcttt
     1381 tgccgccata gaatcgggaa tatcaaacag tacgctcaag ccatagttaa gctgcaccac
     1441 accgataccg agcgtagtgg caataccgaa gatggtgccg atcaccgctg caatatccac
     1501 tgagtgccct atcggcccgt taatccgttt accgaagatc gggtacagcg ccgagcggat
     1561 ggtgagcggc aaattataac gataactaaa gtatccgagc gccatgccca tcagcgcata
     1621 catcgaccag ccggttaagc cgtagtgaaa cagcgtccag accatcgcct gacgcgcggc
     1681 ctcaatcgtc tgtcccgcgc cttccggcgg ctgcatatac tgcgttaccg gttcggctac
     1741 ggagaagaac atcaggtcga taccgatccc ggcagcaaac agcatcgccg cccaactcag
     1801 cagactgaat tccggtttgg attgttctgg cccgagcttc accgaaccaa aacgcgaaca
     1861 agcgatacag accacaaaga caatatagag cgttgccgcc agcagatagt accaaccgaa
     1921 ggttttagaa acccagtcca gcgtgcggcc aatccacagg gccgagaagt cgcgaaacag
     1981 gatcgttgtc cttttccctg ctgtgtgaaa ggtccgtcat ccagtatccc cacttttttg
     2041 ttaccattaa atatcaactt agaaacaatt aagacacatt ttatattgaa cgtccaatca
     2101 ataaccgctt taatagataa acaacgctga tgaatggagt ggcgaaaatg cccaaattgg
     2161 ggatgcagtc gatccggcgc aggcaactga tcgacgccac gctggaagca atcaatgagg
     2221 tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg tgcaggcgtt tctacgggga
     2281 tcatcagcca ctatttcagg gacaaaaatg gtctgctgga agcaaccatg cgcgatatca
     2341 ccagtcagct gcgtgacgcg gttttgaatc gattacatgc acttccgcag ggcagtgcag
     2401 aacaacgatt acaggcgatt gttggcggaa acttcgatga aacgcaggtg agcagtgcgg
     2461 cgatgaaagc ctggctggcg ttctgggcca gcagtatgca tcagccgatg ctctatcgct
     2521 tgcagcaggt cagtagccgc cgcttattgt cgaatctggt gagcgaattt cgccgcgagt
     2581 taccgcgcca acaggcacag gaagcgggct acggccttgc cgcgctgatt gacggattat
     2641 ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc tctcgcacac tcgctgaccc
     2701 gtcactttat tactcagcat ttacccaccg attaaccgag gagacgtgat gtcccgaatg
     2761 gcagaacagc agctttatat acatggtggt tatacctccg ccaccagcgg tcgcaccttc
     2821 gagaccatta acccggccaa cggtaacgtg ctggcgaccg tgcaggccgc cgggcgtgag
     2881 gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa aaatctgggc ggcgatgacc
     2941 gctatggagc gctcgcgtat tctgcgtcgg gccgttgata ttctgcgtga acgcaatgac
     3001 gaactcgcaa aactggaaac cctcgatacc ggaaaagcat attcggaaac ctcaaccgtc
     3061 gatatcgtta ccggtgcgga cgtgctggag tactacgccg ggctgatccc ggcgctggaa
     3121 ggcagccaga tcccgttgcg tgagacgtca tttgtttata cccgccgcga accgctgggt
     3181 gtggtggcgg ggattggcgc atggaactac ccgattcaga ttgccctgtg gaaatccgcc
     3241 ccggcgctgg cggcaggcaa cgcaatgatt ttcaaaccga gcgaagtcac cccgcttacc
     3301 gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc cggacggcgt atttaacgtg
     3361 ttgccggggg tgggcgcgga gaccgggcaa tatctgaccg agcatccggg cattgccaaa
     3421 gtgtcattta ccggcggtgt cgccagcggc aaaaaagtga tggctaactc ggcggcctct
     3481 tccctgaaag aagtgaccat ggaactgggc ggtaaatcac cgctgatcgt tttcgatgat
     3541 gcggatctcg atcttgccgc cgatatcgcc atgatggcaa acttcttcag ctccggtcag
     3601 gtgtgtacca atggcacccg cgtcttcgtt ccggcgaaat gcaaagccgc atttgagcag
     3661 aaaattctgg cgcgcgttga gcgcattcgc gcgggcgacg ttttcgatcc gcaaactaac
     3721 ttaggcccgc tggtcagctt cccgcatcgc gataacgtgc tgcgctatat cgccaaaggc
     3781 aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac tgaaaggcga tggcttcgat
     3841 aacggcgcat gggttgcacc gaccgtgttc accgattgcc gcgacgaaat gaccatcgtg
     3901 cgcgaagaga tcttcggtcc ggtgatgtcg cttctcactt atgaatctga ggacgaagtg
     3961 attcgccgcg ccaatgatac cgactacggc ctggcggcgg gcatcgtgac ggcggacctg
     4021 aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta tttgctggat caacacctgg
     4081 ggcgaatccc cagcagagat gcccgttggc ggctacaaac actccggcat tggtcgcgag
     4141 aacggcgtga tgacgctcca gagttacacc caggtgaagt ccatccaggt tgagatggct
     4201 aaattccagt ccatattcta accaggaggt ttatttgcaa tttgactaca tcattattgg
     4261 tgccggctca gccggcaacg ttctcgctac ccgtctgact gaagatccga atacctccgt
     4321 gctgctgctt gaagcgggcg gcccggacta tcgctttgac ttccgcaccc agatgcccgc
     4381 agcgctggcg ttcccactac agggcaaacg ctacaactgg gcctatgaaa cagagcctga
     4441 accgtttatg aataaccgcc gcatggagtg cggacgcggt aaaggcctgg gtggatcgtc
     4501 gctgatcaac ggcatgtgct acatccgtgg caatgcgctg gatctcgata actgggcgca
     4561 agaacccggt ctggagaact ggagttatct cgactgcctg ccctactacc gcaaggccga
     4621 gactcgcgat gtgggcgaga acgactacca cggtggcgat ggcccggtga gcgtcactac
     4681 ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt gaagcgggcg tgcaggcggg
     4741 ctacccgcgc acggacgatc tcaacggtta tcagcaggaa ggttttggtc cgatggatcg
     4801 caccgtcacg ccgcagggcc gtcgcgccag taccgcgcgc ggttatctcg atcaggccaa
     4861 atcgcgccct aacctgacca ttcgtactca cgctatgacc gatcacatca tttttgacgg
     4921 caaacgcgcg gtgggcgtcg aatggctgga aggcgacagc accatcccaa cccgcgcaac
     4981 ggccaacaaa gaagtgctgt tatgtgcagg cgcgattgcc tcaccgcaga tcctgcaacg
     5041 ctccggcgtc ggcaacgctg aactgctggc ggagtttgat attccgctgg tgcatgaatt
     5101 acccggcgtc ggcgaaaatc ttcaggatca tctggagatg tatctgcaat atgagtgcaa
     5161 agaaccggtt tccctctacc ctgccctgca gtggtggaac cagccgaaaa tcggtgcgga
     5221 gtggctgttt ggcggcactg gcgttggtgc cagcaaccac tttgaagcag gtggatttat
     5281 tcgcagccgt gaggaatttg cgtggccgaa tattcagtac catttcctgc cagtagcgat
     5341 taactataac ggctcgaatg cagtgaaaga gcacggtttc cagtgccacg taggatcgat
     5401 gcgctcgcca agccgtgggc atgtgcggat taaatcccgc gacccgcacc agcatccggc
     5461 gattctgttt aactacatgt cgcacgagca ggactggcag gagttccgcg acgcaattcg
     5521 catcacccgc gagatcatgc atcaacccgc gctggatcaa taccgtggcc gcgaaatcag
     5581 ccccggtgtc gaatgccaga cggatgaaca gctcgatgag ttcgtgcgta accacgccga
     5641 aaccgccttc catccgtgcg gtacctgcaa aatgggttac gacgagatgt ccgtggttga
     5701 cggcgaaggc cgcgtacacg ggttagaagg cctgcgtgtg gtggatgcgt cgattatgcc
     5761 gcagattatc accggcaatt tgaacgccac gacgattatg attggcgaga aaatagcgga
     5821 tatgattcgc gggcaagaag cgctgtcgag gagcacggcg ggatattttg tggcaaatgg
     5881 gatgccggtg agagcgaaaa aatgagtcgt gatttgaact aacgcaggaa ccgatcagat
     5941 tgagagttac cgttccagat atggggaccg aatcgttata taaaacactg aggtaactct
     6001 catgcttcat atccagtatt catgatgctg gttttttgct gatatttaac ccgaacattc
     6061 ctgatacaga ctattaatac aacattatct gcgacagatt ttattttctg gatatattat
     6121 gagtgaacaa atcaaacagg atatcgatct gattgagatt ttatttcatc tgaagaaaaa
     6181 gattcgtgtt atccttttta ttatggctat ctgtatggca atggtgctgt tgtttctgta
     6241 tatcaatata tcaataaaga caatataaaa gtgatttaca gccttaaaat aaaccagaca
     6301 acgccaggta tacttgttag ctgtgatagc aataataatt ttgcctgtca gaccacaatg
     6361 actgaagatg ttattcagcg aattactaca ttttttcaga ccagcccaga tgtcaagaac
     6421 agagaaataa ggctggaatg gtcaggagat aagagagatt taccaactgc tgaagaggaa
     6481 atatctcgcg tgcaggcgtc tattatcaaa tggtatgcgt cagaatacta taatggcagg
     6541 caagttctcg atgagataca aacgccttca gcaattaaca gtgagcttta tacaaaaatg
     6601 atatacctga ccaggaactg gtcattgtat ccgaacggtg atggctgtgt aactataagc
     6661 tcaccagaaa taaaaaataa ataccctgct gccatttgcc tggctctggg attttttcta
     6721 agcattgtaa tttctgtaat gttttgcctt gtcaaaaaaa tggtagatga ataccaacaa
     6781 aactctgggc agtaataaac agcattctga tagagagtgc ctgtataaag ctgaagtgac
     6841 aatgaccggg aagggaaccc gttttcagga atggatgcct gtctttttca ctgtaaatat
     6901 cctggcatta ctaatgccag gatatttaca gaagagcagc ataaccatga cggaacgggg
     6961 ttaagtcagg ttgtccttaa cgctattttc aacctcgtat gccttcttca ggtttatgtt
     7021 cagatctcat atctctctca gcaatcctca ataatcagat acaccgcttt caccggacca
     7081 tgaaccccga cgactttgat aagctcaata tccgccgttg agctgggacc actgatgatg
     7141 ttaatacatg acggcattcg ttcaccggcc tgcgctttct gatgcaattt ttctgcgagt
     7201 tgcgctacac gcggcaggat tgtgctttta cgcaggataa aaagagaaga ttccgggagc
     7261 aggctcaatg aacgcccgcg ctcggcggcg gaaaaaagaa ccacgcctcc cgattcggtt
     7321 aaaccatatt cagcatacac gacacccact ttagcctgct ctgcctgaga gatattctcg
     7381 gcacctttcg ccggatccca aacaacggca ttgcattcct gctgcaaacg ttcactaatc
     7441 cccaattcct ccagcctcgt gtcaccgcta atcacgaccg actgatctcc cagctcttta
     7501 cacagacgta ttgcagcttc tgccgccttc gcctcgctgg tcagctcaca gcgcgtcaac
     7561 ataacatcgc tggcaaactg aataaacgcg tcacagcgct gctgttggtt aagttgggta
     7621 agccgctcgt tagcatagtt gttaagcggc gcatcttctg cttgcggttc aagtcgcagc
     7681 gggcgaccca gtgcctgagc aacgttattc aaaaattcgc tccgattatc cattcttttt
     7741 ctcctgcgcc tgatgtttct taaaccaact acggaaactc tctccgtcag cttcaggaag
     7801 atcgcgtgct tccatccagt cgctaatcgc gccaaatttg agtggtgttt tgccgccatt
     7861 gataaaccag cttgccgcat gagcgccggc catcatcccg actttccaca atcctggatg
     7921 actattggca taagcgaaca tttttatcgc ccgttgctct gcttttgcgg tgatcccttt
     7981 ttcagccatc acccgacgat gacgcaaaat cagttttgac agcggaatac gcaccggaca
     8041 cacgctgtca caagcagtgc ataaagagca ggcgtagggt aaatctttaa aatctttata
     8101 gccgccaagt agcggagaaa tcaccgcacc aattggccct ggataaatag agccatatcc
     8161 atgaccgcca atatggcgat atgccggaca agtattcata caagccccgc agcgaataca
     8221 gcgcagcaca tcccgaaatt cagaggccag cacctcagaa cgcccgttat cgacaataac
     8281 cagatgaaac tcttcaggac catcaacgtg ccccgcttcg cgcggccctg tcagccaggt
     8341 gttgtatccc gtcaaacgtg caccaacggc actgcgcgcc agcatggtga tcaatacatc
     8401 tacctcggca aacgtggggg caatacgctc cattcccatc actgcaatat gcgttttagg
     8461 cagcgtggta cacattcgcg cattaccttc attggtcacc aggcataccg aaccggtctc
     8521 tgccaccgcg aaattacagc cggtaatacc tatttcagca ctgaggaaat cttcacggat
     8581 tttttgccgg atgaataagg tcatcgcttc aggcgtttcc ggcccctcat agcccagacg
     8641 ttcgtgtagc actcgacgga tctgatggcg atctttatga attgccggga ccacaacatg
     8701 agatggcgga tcttgatcca gctggagaat atattcaccc agatcggttt caatcacctg
     8761 aatgccagca tcctgcaaca catgattgac accaatctct tcggtcacca tcgatttaga
     8821 tttcaccacc ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc gggtagcgtc
     8881 ttctttggtt tttgcaaaat agacgtgacc gccgttttgc gtcacttttt ctgagagctg
     8941 gtacagataa gcgtcgagat tactcagaac atgatcacgt atctgggcgg cccgatcgcg
     9001 ccactcctcc cagtgcccca attcatcgac cattttttgc cgatttgccc caatacgctg
     9061 ctgcgcgttt gccaccgctt tgcgcatgat cggatcttca atttgctgac ggatgcgtgt
     9121 cttaaaatct gtattactgg ttttgatcga catctttata tcctcagcgg ctcatcaaca
     9181 cttcagcaat atgcatcact ttgactttct gcccttcccg ttgtaatcgc ccactgatgt
     9241 tcagcaggca actcacgtca gcaccaatta aatactcagg gcggacttcc atcaggtgca
     9301 caaccttttc tttcaccatc tcgccggata tttcggccat tttgaccgag aacgtgccgc
     9361 caaatccgca gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca cgcacatttt
     9421 tcagcagcgt aagtggctcg tccttcactc ccagcttacg ggccaggcta caagatgggt
     9481 gatacacagc tctcccttgc aaactggcac ctacatcgac tacccctaat ttattaacaa
     9541 taaaagaggt gagatcctgc atacgcacgg caaccttttc ggcacgtgat gcccattcag
     9601 gttcatccgc cagatacgtc gggtaacttt ttacggcata gatgcaagag ccagccggtg
     9661 aaataatggg atcgtcgtta tcctccagtg cggcgatcag atttttcatc cctggaatcg
     9721 cttctttgat ataaccgcta ttgatcgcag gctgaccgca gcatccctgt ttctccggga
     9781 aatttacgcg acagccgagt ttttccagta gcagcacgga gtctcgtgcc attcttgatt
     9841 tcagggcgtc accaatacag gtgacaaaga aattgacatt cacaactaat actccattac
     9901 ttcatgccca tttatgcccg atgtttcccc cccaccgggg gatcaatcat caataaatat
     9961 ggcgttcttt tttaattggc tagcaacata ccacccctca tttgtatgac aaatgataat
    10021 ctttcatcca aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt
    10081 tatggggaaa tcgacgatca tttttggcat aagaaatcca tttaaaaatg gctacatccc
    10141 ttatacctat tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa
    10201 aaaaatcacc gacaataatc acgtatcaag taatagtcgg
//
LOCUS       DADKXM010000138        10085 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.139, whole genome shotgun sequence.
ACCESSION   DADKXM010000138 DADKXM010000000
VERSION     DADKXM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.139"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JQR05_003326"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JQR05_003326"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY5325400.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JQR05_003327"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JQR05_003327"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY5325401.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3325)
                     /locus_tag="JQR05_003328"
                     /pseudo
     CDS             complement(2698..3325)
                     /locus_tag="JQR05_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /locus_tag="JQR05_003329"
     CDS             3455..5215
                     /locus_tag="JQR05_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HAY5325402.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYGKLQRAKAAFWAK"
     gene            5621..6478
                     /gene="focA"
                     /locus_tag="JQR05_003330"
     CDS             5621..6478
                     /gene="focA"
                     /locus_tag="JQR05_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY5325403.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6533..8815
                     /gene="pflB"
                     /locus_tag="JQR05_003331"
     CDS             6533..8815
                     /gene="pflB"
                     /locus_tag="JQR05_003331"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY5325404.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9007..9747
                     /gene="pflA"
                     /locus_tag="JQR05_003332"
     CDS             9007..9747
                     /gene="pflA"
                     /locus_tag="JQR05_003332"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY5325405.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9829..>10085)
                     /locus_tag="JQR05_003333"
     CDS             complement(9829..>10085)
                     /locus_tag="JQR05_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325406.1"
                     /translation="GDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYL
                     GIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         2587 a         2530 c         2393 g         2575 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacagac
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttgactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct ggtccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacggaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgttttttt
     5281 aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa ggttaaatat
     5341 cggtaatttg tatttaataa atacgatcga tattgttact ttattcgcct gatgctccct
     5401 tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt taatttttaa
     5461 aatttatttt tatttggata atcaaatatt tactccgtat ttgcataaag accatgcgag
     5521 ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt
     5581 gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt
     5641 tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg tctataaagc
     5701 aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat
     5761 cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct tcggcatggc
     5821 aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc
     5881 cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc gcatcacctg
     5941 gggtcagttg gctaaaaact ggctaaatgt ctattttggc aacctagtcg gcgcactgct
     6001 gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc aatggggact
     6061 aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgtct
     6121 tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag
     6181 cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt
     6241 tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc
     6301 atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt
     6361 gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta tcggcggtgg
     6421 tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg
     6481 gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga
     6541 gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga
     6601 agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt
     6661 cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggcgttaa
     6721 actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt ccaccatcac
     6781 ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc tgcagactga
     6841 agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg
     6901 caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa
     6961 aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc
     7021 cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg gtgactaccg
     7081 tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc agttcacctc
     7141 tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga
     7201 aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta
     7261 cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta
     7321 cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt
     7381 cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca
     7441 ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga
     7501 atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg gtggtatggg
     7561 cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac
     7621 tatggggccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac tgccgctgaa
     7681 cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt atgagaacga
     7741 tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc
     7801 gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat
     7861 gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc
     7921 tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca tggatcactt
     7981 catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga
     8041 caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat
     8101 ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc
     8161 gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga
     8221 atacccgcag tttggtaaca atgatccgcg tgtagatgac ctggctgttg acctggtaga
     8281 acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc
     8341 tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg
     8401 tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa
     8461 aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg
     8521 tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa
     8581 gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg
     8641 tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc
     8701 ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct
     8761 gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga
     8821 tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt
     8881 tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac
     8941 tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac
     9001 accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc
     9061 ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat
     9121 aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttgatgaag
     9181 gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc
     9241 ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg taaaaaagaa
     9301 ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc ggtgattgat
     9361 gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc
     9421 caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa atatctggcg
     9481 aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac
     9541 gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga gaaaatcgaa
     9601 cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga agaatacaaa
     9661 ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag
     9721 cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt attgagttgc
     9781 cgctttttta ttccgcgctt cattctgcta cagcgttttt aacacatctt atagtgcatc
     9841 taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa gttcttcccc
     9901 atcgaatacc actgtattac acaagaaagt gacatcggca tcttcaatac caagataact
     9961 acacatattt tttaaataat cgcttatatt tttttcccag cccatctgga caaatgattc
    10021 tttgtctcct ccaaccagcg ccacccaacg aactttattg aatggtaatt tgtgcccatc
    10081 tccat
//
LOCUS       DADKXM010000139        10022 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.140, whole genome shotgun sequence.
ACCESSION   DADKXM010000139 DADKXM010000000
VERSION     DADKXM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.140"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JQR05_003334"
     CDS             complement(<1..186)
                     /locus_tag="JQR05_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HAY5325407.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINART"
     gene            complement(191..376)
                     /locus_tag="JQR05_003335"
     CDS             complement(191..376)
                     /locus_tag="JQR05_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY5325408.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(373..3019)
                     /locus_tag="JQR05_003336"
                     /pseudo
     CDS             complement(373..3019)
                     /locus_tag="JQR05_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
     gene            3227..4216
                     /gene="ldhA"
                     /locus_tag="JQR05_003337"
     CDS             3227..4216
                     /gene="ldhA"
                     /locus_tag="JQR05_003337"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY5325409.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4327..4749
                     /gene="hslJ"
                     /locus_tag="JQR05_003338"
     CDS             4327..4749
                     /gene="hslJ"
                     /locus_tag="JQR05_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY5325410.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4746..5012)
                     /locus_tag="JQR05_003339"
     CDS             complement(4746..5012)
                     /locus_tag="JQR05_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY5325411.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5286..8810
                     /gene="nifJ"
                     /locus_tag="JQR05_003340"
                     /pseudo
     CDS             5286..8810
                     /gene="nifJ"
                     /locus_tag="JQR05_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            9177..>10022
                     /gene="ompN"
                     /locus_tag="JQR05_003341"
     CDS             9177..>10022
                     /gene="ompN"
                     /locus_tag="JQR05_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY5325412.1"
                     /translation="MKRKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNERDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYVVAN
                     KTQNFE"
BASE COUNT         2552 a         2563 c         2457 g         2450 t
ORIGIN      
        1 cgttcgcgca ttaatttcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac
       61 cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa
      121 tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt
      181 catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg
      241 ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag
      301 gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag
      361 ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg
      421 cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca
      481 cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc
      541 aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt
      601 aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc
      661 agtcatattg tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattgg
      721 cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg
      781 caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag
      841 ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa
      901 ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg
      961 ttcttcttca gtccacggat aacgcccttg caaatcagca gtaatatttt tcgctgtcac
     1021 cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc
     1081 tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat
     1141 ccaggcacta ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc
     1201 agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg
     1261 tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag
     1321 acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc
     1381 gcctttaaag aggaaatagg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg
     1441 cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct
     1501 gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg
     1561 cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag
     1621 cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg
     1681 tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcca gcgtcaggga
     1741 gaaaatcatt cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg
     1801 cctgcaaaat ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta
     1861 aaggccagcg gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta
     1921 acgcgcctgg ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta
     1981 aacgggcata taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt
     2041 gactgttatc catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg
     2101 cttgccccta ggtcagcaca ctcagtcgtc cgctgaccag agcgttctca agccctgcct
     2161 gccagttgtc gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc
     2221 tccagcgacc atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca
     2281 acggatcgcc gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca
     2341 gctcggcatc caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac
     2401 ttacaggaag tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg
     2461 gctgattttc aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct
     2521 ggccttgaaa tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg
     2581 cgagagagag ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga
     2641 tccaggtgtt aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt
     2701 gctccgtctg cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca
     2761 ataaccagcg gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc
     2821 ccaccagata acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat
     2881 ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc cagcgtggga acccacagcc
     2941 cgagcgtcat cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaaagg
     3001 ctttatattt acccagcatg ggtagttaac atcctgattt agcgaaaaat taagcattca
     3061 atacgggtat tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag
     3121 ttgttgatga atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat
     3181 atttttagta gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt
     3241 ttatagcaca aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt
     3301 tgagctggaa ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg
     3361 cgaagcggta tgtattttcg taaacgatga cggcagccgc ccggtgctgg aagagctgaa
     3421 aaagcacggc gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga
     3481 tgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt
     3541 tgctgaacac gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca
     3601 gcgtacccgt gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa
     3661 aacggcaggc gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg
     3721 ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg
     3781 tgtggagtat gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg
     3841 cccgctgaca ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa
     3901 tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga
     3961 agcgctgaaa aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga
     4021 tctgttcttt gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc
     4081 tgcctgccac aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac
     4141 cagtatttct cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc
     4201 gaacgaactg gtttaatctt gccgctcccc tgcaatccag gggagctgat tcagataatc
     4261 cccaatgacc tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga
     4321 accacaatga agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta
     4381 agtaatgaca aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa
     4441 agcgtaaacg gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa
     4501 aaaatgatga tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat
     4561 ggtgaactga cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt
     4621 aatgaactcg ataacaccat tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc
     4681 gcgaaccagt taacgctggc gaccgcgaaa cagacattaa cttataagct ggcggattta
     4741 atgaattaat agctgccaca gctcccggcg gcaagtgact gttcgctaca gcgtttgccg
     4801 ttgggtaatg cacacatccc aatcgccgta ccatccagtt gacgggcaac agaaagcgaa
     4861 ccgccgatca ttgcacaatt tgcttctcca ctactggaca tcgacgcttt taaacctggc
     4921 gctacgtgcg ccgcagtcgc ctgctgaaca ggctcactac tacacgccga caacaataaa
     4981 gcggcacacc ctacccaaaa cgctgctcgc atctcttctt cctctgatct tcaagccaaa
     5041 cgacaccgcc ataaataata ggcagcacag agggcggcgt cgagagctgt cctgcgcgtt
     5101 gccccgcatt tttacttttt tatggctatt tttttgccct ctgtttgatc aaaacattca
     5161 ttactctgat gtgggggaca caaaagcgaa aatgcagaag aaagccattt gctaaaattg
     5221 aaggattact actgggcgcg cagcaatttc gtgcgcccct catttgcgca atgtaagggt
     5281 gtcatatgat tactattgac ggtaatggcg cggttgcttc ggtggcgttt cgcaccagtg
     5341 aagttatcgc catctaccct attaccccca gttccacgat ggcagaacag gctgatgcct
     5401 gggccggaaa cggcttgaag aacgtttggg gagacacacc acgcgtggtt gaaatgcagt
     5461 cggaagcggg tgctatcgtt accgtgcatg gcgctttgca gacgggtgcc ctttcaacat
     5521 cgtttacgtc atcgcagggt ttgctgctga tgatcccgac gatgtacaaa ctggcaggcg
     5581 aactgacgcc atttgtcctg catgtagcgg cacgtaccgt tgccacacat gcactttcta
     5641 tttttggcga tcattccgac gttatggcgg tgcgccagac gggttgcgcg atgttgtgtg
     5701 cggctaacgt ccaggaagcg caagactttg ctctcatttc gcacatcgca acgctgaaga
     5761 gccgcgtgcc atttattcat ttctttgatg gtttccgcac gtcccacgaa atcaataaaa
     5821 ttgtcccgct ggccgatgac actattcttg atctcatgcc gcaggccgaa attgatgctc
     5881 atcgcgcccg ggcgctcaac ccggaacatc cggtgatccg cggtacgtcc gccaatcctg
     5941 acacttattt ccagtctcgc gaagccacca acccctggta caacgcggtc tatgaccatg
     6001 ttgaacaggc gatgaatgat ttcgctgccg cgacaggtcg tcagtatcag ccgtttgagt
     6061 attacgggca tccgcaagcg gaacgggtga ttatcctgat gggctctgcc attggcacct
     6121 gtgaagaagt ggttgatgaa ttactaaccc gtggcgaaaa agttggcgtg ctgaaagttc
     6181 gtctgtaccg tcccttctcc gccaaacact tgctgcaagc tctgccggga tccgtacgca
     6241 gcgtggcggt actggacaga accaaagaac ccggtgccca ggcagaaccg ctctatctgg
     6301 atgtgatgac cgcactggca gaagccttta ataatggtga gcgcgaaacc ctgccccgtg
     6361 tcattggtgg gcgctatggt ctttcatcca aagaatttgg cccggactgt gtactggcgg
     6421 tatttgccga gctcaacgcg gctaaaccta aagcacgctt tacggttggt atttacgatg
     6481 atgtgaccaa tctgtcactg ccgttgccgg aaaacactct gccaaactcg gcgaaacttg
     6541 aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc cgcaaccaaa aacaatatca
     6601 agattatcgg taattccacg ccgtggtacg cacagggcta ttttgtttac gactccaaaa
     6661 aagcgggcgg cctgacggtt tctcaccttc gcgtgagcga acagccgatt cgttccgctt
     6721 atctcatttc ctaggctgat tttgttggct gccaccagtt gcagtttatc gataaatatc
     6781 agatggctga gcgtttaaaa cctggcggca ttttcctgct taatacgccg tacagcgcag
     6841 atgaagtgtg gtcgcgcttg ccgcaggaag ttcaggccgt gttaaaccag aaaaaagcgc
     6901 gcttctatgt gattaacgcg gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta
     6961 ataccgtcat gcagatggct tttttccatc tgacgcaaat tctgcctggc gatagcgccc
     7021 tcgcagaatt gcagggtgcg attgccaaaa gttacagtag caaaggtcag gatctggtgg
     7081 agcgcaactg gcaggctctg gcgctggcgc gtgaatccgt agaagaagtt ccgttgcaac
     7141 cggtaaatcc gcacagcgcc aatcgaccgc cagtggtttc cgatgccgcc cctgatttcg
     7201 tgaaaaccgt aaccgcggcg atgctcgccg ggcttggtga tgccctcccc gtttcggcgc
     7261 tgccgccaga cggcacctgg ccgatgggca ccactcgctg ggaaaaacgc aatatcgccg
     7321 aagagatccc catctggaaa gaggaactct gtacccaatg taaccactgc gtcgccgctt
     7381 gcccacactc agctattcgc gcaaaagtgg taccgcctga agcgatggaa aacgcccctg
     7441 ccagcctgca ttcgctagat gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc
     7501 aggtggcacc ggaagattgc accggttgta acctgtgcgt cgaagtttgc ccggcgaaag
     7561 accgtcagaa tccagagatt aaagccatca atatgatgtc tcgcctggaa catgtcgaag
     7621 aagagaaaat caattacgat ttcttcctca acctgccaga aatcgaccgt agcaaactgg
     7681 aacgtattga tattcgtaca tcgcagctga ttacaccgct gtttgaatat tcaggtgctt
     7741 gctccggttg tggcgagacg ccgtatatta aattgctgac tcagctctat ggtgaccgga
     7801 tgttgatcgc taacgccacc ggctgttctt caatttatgg cggtaacctg ccctctacac
     7861 cgtataccac cgatgccaac ggtcgtgggc cggcatgggc gaactctcta tttgaagata
     7921 atgccgaatt tggccttggt ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc
     7981 gtctgcagga tcaatttgcc gataaaatcc cggcggaatt actgacggcg ttgaaatcag
     8041 acgctacgcc agaggttcgt cgagaacagg tcgcagcttt acgccagcaa ctcaacgatg
     8101 ttgccgaagc acatgaactg ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc
     8161 tgattggtgg cgacggctgg gcttacgata tcggctttgg cggtctggat catgtattga
     8221 gtttgacgga aaacgtcaac attctggtgc tggatacgca atgctattcc aacaccggtg
     8281 gtcaggcgtc gaaagcgaca ccgctgggtg cagtaactaa gttcggcgag cacggcaaac
     8341 gtaaagcgcg taaagatctt ggcgtcagta tgatgatgta cggtcatgtt tatgtggcgc
     8401 agatttctct cggcgcgcag ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt
     8461 atccgggacc atcgctgatc attgcttata gcccgtgtga agagcatggt tacgatctgg
     8521 ctctcagcca cgaccagatg cgccaactca ctgctaccgg cttctggccg ctatatcgct
     8581 ttgatccgcg tcgtgccgat gaaggcaaac tgccgctggc gctggattca cgcccgccgt
     8641 cagaagcact ggaagaaacg ttacttcacg agcagcgttt ccgtcgtctg aattcgcagc
     8701 agccagaagt ggcagaacag ttatggaaag atgctgcagc tgatttgcaa aaacgctatg
     8761 acttcctggc acaaatggcc ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga
     8821 taaggattat ccaattctaa aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     8881 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     8941 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9001 tattatcttc gatacattca ccagacttat tatcatttca gaggaattat ttgattaagg
     9061 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9121 tttcactccc cgaaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9181 aaagaaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9241 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9301 attatttttc tgataattca gcgaaagatg gcgaccagag ctatgcgcgt ctgggtttta
     9361 aaggcgaaac ccaaattaac gatcaactta ctggctacgg tcaatgggaa tacaatattc
     9421 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccggac
     9481 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
     9541 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
     9601 actttatgac tggtcgagcc aatggcgtcg cgacttatcg taatactgat ttcttcggtc
     9661 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaagga gccagtaatg
     9721 gtcaggaagg caccaataac gaacgcgatg ttcgtcatga aaacggtgac ggctggggtc
     9781 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcttctg
     9841 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
     9901 ctgctgggct aaaatacgat gctaacaata tttacctagc aaccatgtat tcagaaacgc
     9961 gtaatatgac cccgtttggc gacagcgatt atgtagttgc gaacaaaacc cagaactttg
    10021 aa
//
LOCUS       DADKXM010000140         9899 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.142, whole genome shotgun sequence.
ACCESSION   DADKXM010000140 DADKXM010000000
VERSION     DADKXM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.142"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..544
                     /locus_tag="JQR05_003342"
     CDS             <1..544
                     /locus_tag="JQR05_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY5325413.1"
                     /translation="TIPRSYLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIAL
                     RIMQQVKATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSWL
                     VQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFE
                     EAPQAYKSAESVVQCLGRPD"
     gene            660..1220
                     /locus_tag="JQR05_003343"
                     /pseudo
     CDS             660..1220
                     /locus_tag="JQR05_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1277..2734)
                     /gene="pepD"
                     /locus_tag="JQR05_003344"
     CDS             complement(1277..2734)
                     /gene="pepD"
                     /locus_tag="JQR05_003344"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY5325414.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2996..3454
                     /gene="gpt"
                     /locus_tag="JQR05_003345"
     CDS             2996..3454
                     /gene="gpt"
                     /locus_tag="JQR05_003345"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY5325415.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3546..4790
                     /gene="frsA"
                     /locus_tag="JQR05_003346"
     CDS             3546..4790
                     /gene="frsA"
                     /locus_tag="JQR05_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY5325416.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4848..5249
                     /locus_tag="JQR05_003347"
     CDS             4848..5249
                     /locus_tag="JQR05_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY5325417.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5288..6343)
                     /gene="phoE"
                     /locus_tag="JQR05_003348"
     CDS             complement(5288..6343)
                     /gene="phoE"
                     /locus_tag="JQR05_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY5325418.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6631..7734
                     /gene="proB"
                     /locus_tag="JQR05_003349"
     CDS             6631..7734
                     /gene="proB"
                     /locus_tag="JQR05_003349"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY5325419.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7746..8999
                     /gene="proA"
                     /locus_tag="JQR05_003350"
     CDS             7746..8999
                     /gene="proA"
                     /locus_tag="JQR05_003350"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY5325420.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDKSAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADSAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9114..9189
                     /locus_tag="JQR05_003351"
     tRNA            9114..9189
                     /locus_tag="JQR05_003351"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9147..9149,aa:Thr,seq:cgt)
     gene            complement(9204..>9899)
                     /locus_tag="JQR05_003352"
     CDS             complement(9204..>9899)
                     /locus_tag="JQR05_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5325421.1"
                     /translation="LRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPE
                     PLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     LSQSKNKEGTNDV"
BASE COUNT         2369 a         2463 c         2593 g         2474 t
ORIGIN      
        1 tactattccg cgcagctatt tagggcagtc tattttacag cggcatattg cctcgttttc
       61 gcatcatggt ttgcctgaag gcagtgacga cgcgctggcg tttgcgcgtt ttaatcgcca
      121 cctgatcgct ttgcgcataa tgcaacaggt taaggccacc ggaagtccgg ttctggatgt
      181 ggcgcataac tttgttagcg cgtgtcgaat cggtgatcaa caaggggtct tacatcgtaa
      241 aggtgccacg cctgatgact gtggtctggt ggttattccc ggttcacgcg gtgattactc
      301 ctggctggtt cagcccgtca ggagtgagga aacattgcat tcgctggcgc atggggctgg
      361 gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca gcgaaataca cagcgacgca
      421 actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac tcatcttcga
      481 agaagcgcca caagcctata aatcggctga aagtgtggtg caatgtctgg gcaggccgga
      541 ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag tggagggaaa
      601 aaaggatgat cttgctataa ctctcctcag tgcaggggcc ggaagaatgt tgtctcgcag
      661 tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc gcggtaacgg
      721 tgctggaaac agaaacaggc cgctactctg acacgctgcg ttcggcgctg atttctctgg
      781 atggcgacaa cgcatgggcg ttaagcgaaa gctggtgtgg cactattcag tggatttgcc
      841 cgagcccgta tcgcccacat cacgggcgta aaaactggtt tctgggcatt gggcgtttta
      901 ccgctgatga gcaggagcaa tccgatgcga tccggtatga gacgctgcgt tcgtcggggc
      961 cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat ttggcatccg
     1021 gtattagcgt gaaggttcag tcagagcgca gccagcatgc taacaaacgg ctggcgcgat
     1081 tgctgattgc ctggaagctg gatcagcagc aataggaaaa tagcgcggtg ctgaaatcac
     1141 agcggcgaat gttccatcac cagattgaac gtggcaaccc gcgacgaacg tttacaggga
     1201 tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa acgccatccg
     1261 gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc agcagtgtcc
     1321 agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga ccggtgatag
     1381 ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga ccacattcta
     1441 ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa caggcgctga taggtttcac
     1501 gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat gcgcctttcg
     1561 cttcggtttt cgcgccagcc agtttaccca gcgaatccag catgctcacc acgtagtctt
     1621 taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg tcagtcatgg
     1681 tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg gaattgcgga
     1741 tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat ttcgcaatca
     1801 gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc tctttctctg
     1861 ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagt gcgtcgactt
     1921 tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc
     1981 cgccgttgaa atcgataagg cgcaggtcca gttcttctgc atgacccgcc aggaagcgca
     2041 ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag tgaccgcctt
     2101 tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct tcacgatcta
     2161 aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt tcaccttctt
     2221 cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt aagccgaacg
     2281 caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca
     2341 cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta tccgcaccca
     2401 gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata ggatctttcg
     2461 tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg gcctgtaaga
     2521 cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg atattaccta
     2581 cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg tattcagcga
     2641 gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa atatcccaca
     2701 gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc gatcacccgc
     2761 aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag acaggcttgc
     2821 gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     2881 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     2941 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     3001 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     3061 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     3121 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3181 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3241 aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga
     3301 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     3361 tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg
     3421 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cattgccggg
     3481 cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg
     3541 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     3601 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
     3661 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     3721 gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg
     3781 aacgtaccga cgatgattta tacgataccg tgattggcta ccgtggtggc aactggattt
     3841 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa gaagatccgc
     3901 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
     3961 atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag
     4021 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg
     4081 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
     4141 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
     4201 cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat
     4261 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
     4321 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
     4381 ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
     4441 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
     4501 acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc
     4561 tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca acgccaatgt
     4621 tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct
     4681 catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg
     4741 acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta
     4801 aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga
     4861 gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg
     4921 aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga
     4981 aaccggcacc ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat
     5041 cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg
     5101 ttccggtaaa agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg
     5161 agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg gatgattttc
     5221 gtgatgaacc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca
     5281 atattcatta aaactgatac gtcatcccaa ccgcgacagt atcatcatta ttaatattaa
     5341 gcttattatc gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt
     5401 tgaaataata cgtagcaccg acgtcgatat aattgaccag atcttcatca ccgatgccct
     5461 caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact
     5521 ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg
     5581 tcattttgcg tgtttcagaa tagaaagttg ccagataaat attattggca tcgtatttca
     5641 gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt
     5701 tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg ccaaagtcat
     5761 atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt
     5821 tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc
     5881 gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggaat
     5941 cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt
     6001 tacgaccata atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg
     6061 ttttttgctg tgcagtatca ctctccgctt tatttccggc aaactccgct tcccaccgac
     6121 cataaccagt cagttgatcg ttaatttgag tttcaccttt aaaaccaaaa cggatataac
     6181 tctggtcgcc atctttactg gcgttatcac tcatataatg catggcttta actttgccat
     6241 agacatccag tttattaccg tctttattat atatttctgc agcctgtaca gatgcagatg
     6301 ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta
     6361 attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga
     6421 gattgttaaa gatatattac agattaatga tattctgaaa atgtggtaat ttattaaatc
     6481 tgtaataaaa gcgtaaacaa ctgccgctac gcttgctgat cccgcgcaac aaaacgccat
     6541 gctttgctcg cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc
     6601 atttttccta aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa
     6661 ctcggcacca gtgtgctaac aggcggatcg cgccgtctga accgtgccca tatcgttgaa
     6721 cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg
     6781 ggcgcgatcg ccgccggacg tgagcacctg ggttacccgg aactgccagc gactatcgcc
     6841 tcgaaacaac tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg
     6901 ttttcgattt atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac
     6961 cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt
     7021 ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaggtcgg cgataacgat
     7081 aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat
     7141 caaaaaggtt tgtataccgc tgatccgcgc agcaatccgc aggcagaact gattaaagat
     7201 gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
     7261 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac
     7321 accattattg ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc
     7381 gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc
     7441 ggtgcgccgc cggcgggtga aatcacggta gatgaagggg caactgccgc cattctggaa
     7501 cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt
     7561 gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac
     7621 aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg
     7681 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct
     7741 ggccgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
     7801 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
     7861 gcgaaatcat cctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta
     7921 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg
     7981 atgtacgtca ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac
     8041 tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt
     8101 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
     8161 cggtgatcct gcgtggtggt aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga
     8221 ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc
     8281 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
     8341 cgcgtggtgg cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca
     8401 caggtggtat aggcgtatgc catatttacg ttgataaaag tgcagagatc gctgaagcat
     8461 taaaagtgat cgtcaatgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt
     8521 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
     8581 aaagcggcgt gacattacac gcagattcag ctgcgctgac acagttgcag gcaggccctg
     8641 cgaaggtggt ggcggttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga
     8701 acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
     8761 aacactccga tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag
     8821 tggattcgtc cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg
     8881 gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc
     8941 tggaagcact gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa
     9001 taaaaccggg tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg
     9061 ttagttttaa ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata
     9121 tagctcagtt ggtagagcag cgcattcgta atgcgaaggt cgtaggttcg actcctatta
     9181 tcggcaccat taaaatcaat aagttacaca tcattagtac cttccttatt ttttgactgg
     9241 gacaaatttg ggaccgatgg gttcaggatc gagtctattt gccgtgcgtg ttcggtaagg
     9301 tgattaggtg caaggtgagc atatcgacga accatttcga tagactccca gcctcccatt
     9361 tcctgtaaca ctgacaacgg gactccggct tgaaccagcc aacttgccca ggtgtgtctc
     9421 aagtcgtgaa atctgaaatc atcaatacca gcccgtctca gcgccgcttt ccaggctgtg
     9481 tttgcgtcat accgcatctt ccttactgtt ggcgctttcg ttccgtctgg tttggtacag
     9541 ctttccttgt acacaaatac ccaacggtga tgattcccga tttgtttttt caaaacgcga
     9601 catgcagtat cattcagcgc aacgccgatt gcgcggtttg atttactctc ttccgggttt
     9661 atccatgcca cccggcgctg catatctatt tgttgccatt caaggttgat gatgttcgag
     9721 cgtcttaagc ctgttgccag tgcaaattca acaacagact ttaatggctc cggacattca
     9781 tcaatcagcc tttgtgcttc atggggctcc agccagcgga tccgtttatt ctttggttga
     9841 ggcactttaa taattggtgc cttatccagc attttccatt cacgctctgc ggctcttag
//
LOCUS       DADKXM010000141         9628 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.143, whole genome shotgun sequence.
ACCESSION   DADKXM010000141 DADKXM010000000
VERSION     DADKXM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.143"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            559..2127
                     /gene="cydA"
                     /locus_tag="JQR05_003353"
     CDS             559..2127
                     /gene="cydA"
                     /locus_tag="JQR05_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY5325422.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2143..3282
                     /gene="cydB"
                     /locus_tag="JQR05_003354"
     CDS             2143..3282
                     /gene="cydB"
                     /locus_tag="JQR05_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY5325423.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3297..3410
                     /gene="cydX"
                     /locus_tag="JQR05_003355"
     CDS             3297..3410
                     /gene="cydX"
                     /locus_tag="JQR05_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY5325424.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3410..3703
                     /gene="ybgE"
                     /locus_tag="JQR05_003356"
     CDS             3410..3703
                     /gene="ybgE"
                     /locus_tag="JQR05_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY5325425.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3853..4257
                     /gene="ybgC"
                     /locus_tag="JQR05_003357"
     CDS             3853..4257
                     /gene="ybgC"
                     /locus_tag="JQR05_003357"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY5325426.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4254..4946
                     /gene="tolQ"
                     /locus_tag="JQR05_003358"
     CDS             4254..4946
                     /gene="tolQ"
                     /locus_tag="JQR05_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY5325427.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4950..5378
                     /gene="tolR"
                     /locus_tag="JQR05_003359"
     CDS             4950..5378
                     /gene="tolR"
                     /locus_tag="JQR05_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY5325428.1"
                     /translation="MARARGRGCRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5443..6684
                     /gene="tolA"
                     /locus_tag="JQR05_003360"
     CDS             5443..6684
                     /gene="tolA"
                     /locus_tag="JQR05_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY5325429.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            6817..8109
                     /gene="tolB"
                     /locus_tag="JQR05_003361"
     CDS             6817..8109
                     /gene="tolB"
                     /locus_tag="JQR05_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY5325430.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGLGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8144..8665
                     /gene="pal"
                     /locus_tag="JQR05_003362"
     CDS             8144..8665
                     /gene="pal"
                     /locus_tag="JQR05_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY5325431.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8675..9466
                     /gene="cpoB"
                     /locus_tag="JQR05_003363"
     CDS             8675..9466
                     /gene="cpoB"
                     /locus_tag="JQR05_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY5325432.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNATIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
BASE COUNT         2371 a         2278 c         2578 g         2401 t
ORIGIN      
        1 atttatgcct ttattaataa tcctgaaact ctgcgtcgta ttagccagtg accaaaaaaa
       61 gaattaaggt caaccgtgct gtttttgctt cgtctctttt tatctttaat tgccaaccga
      121 aactaatttc agccttataa ttcacacatt ttaaacataa atgtcactaa agttacctta
      181 ttgaaacatg attaacataa tttgtagaaa ttgatattta tcaatgtata agtcttggaa
      241 atgggcatca aaaagagata aattgttctc gatcaaattg gctgaaaggc ggcaatttag
      301 ctataaattg atcaccgtcg aaaaatgcaa atttgcttca acaaaaacct gtttattgta
      361 aggattttgc ggcgtaatat atacgtggga tcaatttgag tttttattaa catgtttgca
      421 acctttcttt acgccgtttt tgtgtgcatt cacatggtat gatgaaagtg ttcaaacaaa
      481 tttctattgg ggcatgcgtg tgaccctttc taacggggtt cactctcgga gtcttcatgc
      541 gatgagcaag gagtcatgat gttagatata gtcgaactgt cgcgcttaca gtttgccttg
      601 accgcgatgt accacttcct ttttgtgcca ctgacgctcg gtatggcgtt cctcctggcc
      661 attatggaaa cggtctacgt cctctccggc aaacagattt ataaagatat gaccaagttc
      721 tggggcaagt tgtttggtat caacttcgct ctgggtgtgg ctaccggtct gaccatggag
      781 ttccagttcg ggactaactg gtcttactat tcccactatg taggggatat cttcggtgcg
      841 ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat ccacctttgt aggtctgttc
      901 ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt gtgtcacctg gctggtggcg
      961 ctcggttcaa acctgtccgc actgtggatt ctggttgcga acggctggat gcaaaaccca
     1021 atcgcgtccg atttcaactt tgaaactatg cgtatggaga tggtgagctt ctccgagctg
     1081 gtgcttaacc cggttgctca ggtgaaattc gttcacactg tagcgtctgg ttatgtgact
     1141 ggcgcgatgt tcatcctcgg tatcagcgca tggtatatgc tgaaaggtcg tgacttcgcc
     1201 ttcgctaaac gctcctttgc tatcgctgcc agcttcggta tggctgctgt tctgtctgtt
     1261 attgttctgg gtgatgaatc cggctacgaa atgggcgacg tgcagaaaac caaactggct
     1321 gctattgaag ccgagtggga aacgcaacct gcgcctgctg cctttactct gttcggcatt
     1381 cctgatcagg aagaggagac gaacaaattt gcgattcaga tcccttacgc actgggcatc
     1441 attgcaacgc gttccgtgga taccccggtt atcggcctga aagagctgat ggtgcagcat
     1501 gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc tcgaacaact gcgttctggt
     1561 tctaccgacc aggcggttcg tgaccagttc aatagcatga agaaagacct cggttacggt
     1621 ctgctgctga aacgctatac gccaaacgtg gctgatgcga ctgaagcgca gattcaacag
     1681 gcaaccaaag actccatccc gcgtgtagcg ccgctgtact ttgcgttccg tatcatggtg
     1741 gcgtgtggct tcctgctgct ggcaatcatc gcgctctctt tctggagtgt catccgcaac
     1801 cgcattggcg agaaaaaatg gcttctgcgc gccgcgctgt acggtattcc gctgccgtgg
     1861 attgctgtag aagcgggctg gttcgtggct gaatatggcc gccaaccgtg ggctatcggt
     1921 gaagtgctgc cgacagctgt ggcgaattcg tcactgaccg caggcgatct catcttctca
     1981 atggtgctga tttgcggcct gtataccctg ttcctggtgg cagaattgtt cttaatgttc
     2041 aagtttgcac gcctcggccc aagcagcctg aaaaccggtc gctatcactt tgagcagtct
     2101 tccacgacta ctcagccggc acgctaagac aggagtcgtc aaatgatcga ttatgaagta
     2161 ttgcgtttta tctggtggct gctggttggc gttctgctga ttggttttgc agtcactgac
     2221 ggtttcgaca tgggggtggg catgctcacc cgtttcctcg gtcgtaacga caccgagcgt
     2281 cgaattatga ttaactccat tgcaccacac tgggacggta accaggtttg gctgatcacc
     2341 gcgggcggcg cactctttgc tgcctggccg atggtctatg ccgctgcgtt ctccggcttc
     2401 tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc gtccggtcgg ttttgactac
     2461 cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg actggggcat cttcattggt
     2521 agcttcgttc cgccgctggt aattggtgta gcgttcggta acctgttgca gggcgtaccg
     2581 ttcaacgttg atgaatatct gcgtctgtac tacaccggta acttcttcca gttgcttaac
     2641 ccgttcggcc tgctggcagg cgtggtgagc gtagggatga tcattactca gggcgcaacc
     2701 tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta cccgtgcaac ggctcaggtg
     2761 gctgcgctgg tgacactggt ctgtttcgca ctggctggcg tatgggtgat gtacggtatc
     2821 gatggttatg tcgtgaaatc gacaatggac cattacgcag cctctaaccc actgaataaa
     2881 gaagtggttc gtgaagctgg cgcatggctg gttaacttca acaacacgcc aattctgtgg
     2941 gctattccgg cactgggtgt ggttctgccg ctgctgacca tcctgactgc acgtatggat
     3001 aaagccgcgt gggcgtttgt gttctcctcc ctgacgctgg cctgcatcat cctgacagcc
     3061 ggtatcgcaa tgttcccgtt tgtgatgccg tccagcacca tgatgaacgc aagtctgaca
     3121 atgtgggatg caacttccag ccagctgacg cttaacgtca tgacctgggt tgcggtggtt
     3181 ctggtaccga tcattctgct ctacaccgcc tggtgttact ggaaaatgtt cggtcgtatc
     3241 accaaagaag atattgaacg taacacccac tctctgtact aagtaaggag ctaaaaatgt
     3301 ggtatttcgc atggattctg ggaacgcttc ttgcctgttc gtttggggta atcaccgcgc
     3361 tggcgcttga acacgtcgaa tcaggcaaag ccggtcaaga agacatctga tgagtaagat
     3421 tatcgcgacc ttgtatgcgg taatggacaa gcgccccctg cgggcgcttt ccttcgtgat
     3481 ggcgcttctg ttagcaggat gtatgttttg ggacccatct cgttttgccg cgaagaccag
     3541 tgaactggaa atctggcatg gtttattgct gatgtgggcc gtctgtgctg gtgtgattca
     3601 cggcgtgggc tttcgtccgc agaaggttct ttggcaaggg attttttgcc cattgcttgc
     3661 cgatattgtt ctcattgtcg ggctgatttt cttcttcttt taaatcagaa ttctctttaa
     3721 aaaattatgg gccgctccag gcccataaat ttttacgctc ccttaacttg ccctcattcc
     3781 caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag ctctaacttt tgttgcatta
     3841 ccgggatgta aagtgaatac aacgctgttt cgatggccgg ttcgcgtcta ctatgaagat
     3901 accgatgccg gtggtgtggt gtaccacgcc agttacgtcg ctttttatga aagagcacgc
     3961 acagagatgc tgcgtcatca tcacttcagt cagcaggcgc tgatggctga acgcgttgcc
     4021 tttgtggtac gtaaaatgac ggtggaatat tacgcacctg cgcggctcga cgatatgctc
     4081 gaaatacaga ctgaaataac atcaatgcgt ggcacctctt tggttttcac gcaacgtatt
     4141 gtcaacgccg agaatacttt gctgaatgaa gcagaggttc tggttgtttg cgttgaccca
     4201 ctcaaaatga agcctcgtgc gcttcccaag tctattgtcg cggagtttaa gcagtgactg
     4261 acatgaatat ccttgatttg ttcctgaagg ctagccttct ggttaaactt atcatgttga
     4321 ttttgattgg tttttcaatc gcatcctggg ccattattat ccagcgaacc cgtattctta
     4381 acgcagcggc gcgtgaagcc gaagcgtttg aagataaatt ctggtctgga atcgaactct
     4441 ctcgcctcta tcaagagagt caggggaaac gggataatct gactggttcg gaacaaatct
     4501 tttacagcgg gttcaaagag tttgtgcgcc tgcatcgtgc caatagccat gcgccggaag
     4561 ccgtagtgga aggggcgtcg cgtgctatgc gtatctccat gaaccgtgaa cttgaaaatc
     4621 tggaaacgca cattccgttc ctcggtacgg ttggctccat cagcccgtat attggtctgt
     4681 ttggtacggt ctgggggatc atgcacgcct ttatcgccct cggggcggta aaacaagcaa
     4741 cactgcaaat ggttgcgccc ggtatcgcag aagcgttgat tgcgacggca attggtctgt
     4801 tcgccgcaat tccggcggta atggcttata accgtctcaa ccagcgcgta aacaaactgg
     4861 aactgaatta cgacaacttt atggaagagt ttaccgcgat tctgcaccgc caggcgttta
     4921 ccgttagcga gagcaacaag gggtaagcca tggccagagc gcgtggacga ggttgtcgcg
     4981 atctcaagtc cgaaatcaac attgtaccgt tgctggacgt actgctggtg ctgttgctga
     5041 tctttatggc gacagcgccc atcatcaccc agagcgtgga ggtcgatctg ccagacgcta
     5101 ctgaatcaca ggcggtgagc agtaacgata atccgccagt gattgttgaa gtgtctggta
     5161 ttggtcagta caccgtggtg gttgagaaag atcgcctaga gcgtttacca ccagagcagg
     5221 tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa aacggtcttt ctgatcggtg
     5281 gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact gaacttgtta catagtgcgg
     5341 gtgtgaaatc ggttggttta atgacgcagc ctatctaaac atctgcgttt cccttgcttg
     5401 aaagagagcg ggtaacaggc gaacagtttt tggaaaccga gagtgtcaaa ggcaaccgaa
     5461 caaaacgaca agctcaagcg ggcgataatt atttcagcag tgctgcatgt catcttattt
     5521 gcggcgctga tctggagttc gttcgatgag aatatagaag cttcagccgg aggcggcggt
     5581 ggttcgtcca tcgacgctgt catggttgat tcaggtgcgg tagttgagca gtacaaacgc
     5641 atgcaaagcc aggaatcaag cgcgaagcgt tctgatgaac agcgcaagat gaaggaacag
     5701 caggctgctg aagaactgcg tgagaaacaa gcggctgaac aggaacgcct gaagcaactt
     5761 gagaaagagc ggttagcggc tcaggagcag aaaaagcagg ctgaagaagc cgcaaaacag
     5821 gccgagttaa agcagaagca agctgaagtg gcggcagcga aagcggcggc agatgctaaa
     5881 gctgcggaag aagcagcgaa gaaagcggct gcagacgcga agaaaaaagc agaagcagaa
     5941 gccgccaaag ccgcagccga agcgcagaaa aaagccgagg tagccgctgc ggcactgaag
     6001 aagaaagcgg aagcggcaga agcagctgca gctgaagcaa gaaagaaagc ggcaactgaa
     6061 gctgctgaaa aagccaaggc agaagctgag aagaaagcgg ctgctgaaaa ggctgcagca
     6121 gataagaaag cggcagcaga gaaagctgca gccgacaaaa aagcagcaga aaaagcggct
     6181 gctgataagg cagcagctga taagaaagca gcggcagaaa aagccgccgc agacaaaaaa
     6241 gcggcagcgg caaaagcagc agctgaaaaa gccgctgcag caaaagctgc cgcagaggca
     6301 gatgatattt tcggtgagct aagctctggt aagaatgcac cgaaaacggg gggaggggcg
     6361 aaagggaaca atgcttcgcc tgccgggagt ggtaatacta aaaacaatgg cgcatcaggg
     6421 gccgatatca ataactatgc cgggcagatt aaatctgcta tcgaaagtaa gttctatgac
     6481 gcatcgtcct atgcaggcaa aacctgtacg ctgcgcataa aactggcacc cgatggtatg
     6541 ttactggata tcaaacctga aggtggcgat cccgcacttt gtcaggctgc gttggcagca
     6601 gctaaacttg ctaagatccc gaaaccacca agccaggcag tatatgaagt gttcaaaaac
     6661 gcgccattgg acttcaaacc gtaatcgcga tgttgactgt tcggacggtc aacatcaggc
     6721 accggttgcc acggggttct ggtagttttg tgtattttag tttgttaaca ttctgctaaa
     6781 ttatcgtggg ccatcggtcc agataaggga gatatgatga agcaggcatt acgagtagca
     6841 tttggttttc tcatactgtg ggcatcagtt ctgcatgctg aagtccgcat tgtgatcgac
     6901 agcggtgtag attccggtcg tcctattggt gttgttcctt tccagtgggc ggggcttggt
     6961 gcggcacctg aagatattgg cggcatcgtt gctgctgact tgcgtaacag cggtaaattt
     7021 aatccgttag atcgcgctcg tctgccacag cagccgggta gtgcgcagga agtacaacca
     7081 gctgcatggt ccgcactggg cattgacgct gtagttgtcg gtcaggtcac tccgaatccg
     7141 gatggttctt acaatgttgc ttatcaactt gttgacactg gcggcgcacc gggtactgta
     7201 cttgctcaga actcgtacaa agtgaacaag cagtggctgc gttatgctgg tcataccgcc
     7261 agtgatgaag tgtttgaaaa actgaccggc attaaaggtg cgttccgtac ccgtattgcc
     7321 tacgttgttc agaccaacgg cggtcagttc ccgtatgaac tgcgcgtatc tgactatgac
     7381 ggttacaacc agtttgtcgt tcaccgttca ccgcagccgc tgatgtctcc ggcgtggtca
     7441 ccagacggtt ctaaactggc ttatgtgacc ttcgaaagcg gtcgttccgc gctggttatt
     7501 cagacgctgg caaatggcgc tgtacgtcag gtggcttcat tcccgcgtca caacggtgca
     7561 cctgcattct cgccagacgg cagcaaactg gcattcgcct tgtcgaaaac cggtagtctg
     7621 aacctgtacg taatggattt ggcttctggt cagatccgcc aggtgactga tggtcgcagt
     7681 aacaataccg aaccgacctg gttcccggat agccagaacc tggcatttac ttctgaccag
     7741 gccggtcgtc cgcaggttta taaagtgaat atcaacggcg gtgcgccaca acgtattacc
     7801 tgggaaggtt cgcagaacca ggatgcggat gtcagcagcg acggtaaatt tatggtaatg
     7861 gtcagctcca atggtgggca gcagcacatt gccaaacaag atctggcaac gggaggcgta
     7921 caagttctgt cgtccacgtt cctagatgaa acgccaagtc tggcacctaa cggcactatg
     7981 gtaatctaca gctcttctca ggggatggga tccgtgctga atttggtttc tacagatggg
     8041 cgtttcaaag cgcgtcttcc ggcaactgat ggacaggtca aattccctgc ctggtcgccg
     8101 tatctgtgat aataattaat tgaatagtaa aggaatcatt gaaatgcaac tgaacaaagt
     8161 gctgaaaggg ctgatgattg ctctgcctgt tatggcaatt gcggcatgtt cttccaacaa
     8221 gaacgccagc aatgacggca gcgaaggcat gctgggtgcc ggcactggta tggatgcgaa
     8281 cggcggcaac ggcaacatgt cttccgaaga gcaggctcgt ctgcaaatgc aacagctgca
     8341 gcagaacaac atcgtttact tcgatctgga caagtacgat atccgttctg acttcgctca
     8401 aatgctggat gcacatgcaa acttcctgcg tagcaacccg tcttacaaag tcaccgtaga
     8461 aggtcacgcg gacgaacgtg gtactccgga atacaacatc tccctgggtg aacgtcgtgc
     8521 gaacgccgtt aagatgtacc tgcagggtaa aggcgtttct gcagaccaga tctccatcgt
     8581 ttcttacggt aaagaaaaac ctgcagtact gggtcatgac gaagcggcat actccaaaaa
     8641 ccgtcgtgcg gtactggttt actaagagaa ttgcatgagc agtaacttca gacatcaact
     8701 attgagtctg tcgttactgg ttggtatagc ggccccctgg gccgcttttg ctcaggcacc
     8761 aatcagtagt gtcggctcag gctcggtcga agaccgcgtc actcaacttg agcgtatttc
     8821 taatgctcac agccagcttt taacccaact ccagcaacaa ctttctgata atcaatccga
     8881 tattgattcc ctgcgtggtc agattcagga aaatcagtat caactgaatc aggtcgtgga
     8941 gcggcaaaag cagatcctgt tgcagatcga cagcctcagc agcggtggtg cagcggcgca
     9001 atcaaccagc agcgatcaaa gcggtgcggc ggcatcaacg acgccgacag ctgatgctgg
     9061 tactgcgaat gctggcgcgc cggtgaaaag cggcgatgca aacacggatt acaatgcaac
     9121 tattgcgctg gtgcaggata aatcccgcca ggatgacgca atggtggcat ttcagaattt
     9181 catcaaaaat taccctgatt caacttacct gccaaacgcc aattattggc tgggtcagtt
     9241 aaactacaac aagggtaaaa aagatgatgc ggcgtactat tttgcttcgg tagtgaaaaa
     9301 ctatccgaag tcaccaaagg ctgctgatgc gatgtttaaa gtcggcgtca tcatgcagga
     9361 caaaggtgac accgcaaaag cgaaagccgt ataccagcag gttatcagta aataccctgg
     9421 tactgatggc gctaaacagg cacaaaaacg tctgaacgcg atgtaatgca taacacacga
     9481 ccagaagtcg cattatttct ggtcgtgtcg tgcgaatcat aagcagttga gtgatctgca
     9541 tcgaaatttt tgttgcgctc aagtctgaaa tcagtaatat atgccgccgt tgccacggga
     9601 tatcaaacaa accgaaagca acgaaaaa
//
LOCUS       DADKXM010000142         9460 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.144, whole genome shotgun sequence.
ACCESSION   DADKXM010000142 DADKXM010000000
VERSION     DADKXM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.144"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            100..252
                     /locus_tag="JQR05_003364"
     CDS             100..252
                     /locus_tag="JQR05_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5325433.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            603..755
                     /locus_tag="JQR05_003365"
     CDS             603..755
                     /locus_tag="JQR05_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5325434.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(877..1497)
                     /gene="entD"
                     /locus_tag="JQR05_003366"
     CDS             complement(877..1497)
                     /gene="entD"
                     /locus_tag="JQR05_003366"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY5325435.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JQR05_003367"
     CDS             complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JQR05_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY5325436.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADARDINQGHQFARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4137..5339
                     /gene="fes"
                     /locus_tag="JQR05_003368"
     CDS             4137..5339
                     /gene="fes"
                     /locus_tag="JQR05_003368"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY5325437.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5342..5560
                     /locus_tag="JQR05_003369"
     CDS             5342..5560
                     /locus_tag="JQR05_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY5325438.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5557..9438
                     /gene="entF"
                     /locus_tag="JQR05_003370"
     CDS             5557..9438
                     /gene="entF"
                     /locus_tag="JQR05_003370"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY5325439.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQWYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGCSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         2054 a         2487 c         2611 g         2308 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa ccagacaatt
       61 tggatggtag tcccttcttc gcatggaggc aatataaaca tgctgacgaa atatgccctt
      121 gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggt cggagactcg
      181 ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct cgcttacgaa
      241 ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc aggtcaggat
      301 ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg cgatttcgtt
      361 ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact gtataaataa
      421 acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt tccggtttca
      481 ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca atcctggggc
      541 tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga ggcaatataa
      601 acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg gtgctgggat
      661 ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt aatattgagt
      721 ttaaggctgt tctcgcttac gaaccgaaga agtagccgtt gtgcggggag taatccccgc
      781 atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc ccgcagtcag
      841 tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc acagagttat
      901 gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac gatgaatgat
      961 gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat cagtttggat
     1021 ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgcc agtgtcagcg ccagagaaaa
     1081 ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa
     1141 ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg gttgacggga
     1201 aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct ccgcaggcca
     1261 caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt cccgcaaggc
     1321 ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc cagcgtgttg
     1381 cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg ccggatcgaa
     1441 ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag ttttcatatc
     1501 gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
     1561 tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccgcatc cggcgtgaac
     1621 gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag tgagtattaa tgctcatata
     1681 ccacgtacgt cccggctcgt tataggtata cgcgccggca ccggcgatat agttggctcc
     1741 tgccagatcg cccgtggtct gggcattacc tgcacgccac aaacgtttgt cgaacaggtt
     1801 gtccacgccg ccggtcagac tgacattctt cgtcacatcc caggtcgcgc tcagacccac
     1861 aatgctgtaa ggactgattt ctttggtttc cgggccaacc gctggctgac ctttatagtt
     1921 gtacttcttc ggctgctgct tgccgtacca ggtgaaggtc gtttgcatcg acaaatcttc
     1981 ccgtgcctgc cagctcagcg ttgagttcaa cgtgtactcc gggatgatcg acaaacggtc
     2041 gcccgtggtt ttgttttcac tcttcagcat ataagtgatg ttattggtcc acatcaccgt
     2101 ttcgctaact ggtacgttta acgatccttc cagaccttca accactgctt tcggcacgtt
     2161 atcccactga tagagatcag tgccgactgc gttttgccct acagccacat agcccgcttc
     2221 aatcttattg cgataatcgt tacggaacca ggtgacgccc gccagccagc cgtcgcgttt
     2281 gaactccaga ccaatctctt tgttgatgct ggtttctgct ttcaggtcat cgttaccttg
     2341 cagatagcag ccgcccgcgc tggcatagca gccctgacct ttactgtaga gaatgtagtt
     2401 cgggttagtc tggtacaggc tcggcgcttt ataggcgcgg gcaatgccta ttttcagcgt
     2461 gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg ctccagttat tgccgacaat
     2521 actgtgatga tcgaaacgca gccccggcgt tacgatggtg ctgtcagtca gctccatgtt
     2581 gttttcggca aacagcgaga aaatttctgc ttttgaatac gggctacggt cggtggcact
     2641 cacgccatca atagcgccac cggtattcgt tccggtcagt gcctgggtgt tggaacttaa
     2701 gtccttcatc cgttgctgat tccactccgt acccagcgtc agcgtctggt taacgaggaa
     2761 atcaatcggc aggttaactt cgctgtgcag catcacgtca tcgagatcga tatcgacgaa
     2821 atcctgtgtc gctttttcgt taaatttccc ttcggtaccg cccgccagac cttccggaat
     2881 acgcgagtta cgggtgtgtt catactgcac ccagttgctg gtggtcacgc cgttatccca
     2941 gccaccgttc caggtcagcg cgtagttctg gcgatacagg cggttggttt catcgccata
     3001 tttcgagcga gtgtaagcgt cagagttggt gttctgggta tcgcccgcat acaggttacc
     3061 ctggcggctg taacccgctt ccagttccag cgattgcaat ggtgcgaagt cccagcgcac
     3121 cacgccgtta atatctttat tgattacccc ttcgcgcccg gctggtaacg tcgtggcata
     3181 cgttccggca cgcgcgaact gatggccctg gttgatatcc ctcgcgtcag cctgagtttt
     3241 gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc agcggaccgg tcaggctaaa
     3301 gttagtgcgt ttggtggcac cttcctcttt atgttctggc gcattgaaat atgcgtccca
     3361 ggagccgtgc cactcgccgc tgcctttttt ggtaatgatg ttaaccacgc cgcccgccgc
     3421 gccgttgcca taacgcgcag ctgccggacc acgcagaact tcaatacgtt caatcatttc
     3481 aggtggcacc caggaggtat caccacgggt atcgcgctcg ccacgccagc cctgacgcac
     3541 cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc agcgtgtttt ccggacccat
     3601 accgcgaata tcaatctgtc ggttattccc acgctgacca ctggtggagt taccggtcag
     3661 gttaacgcct ggcatggtac ggatgatctc cgacacatcg cgggcaaccg ggtttttgcg
     3721 gatttcatct gcggtgatgg tcgaaacgcc aggtgcctgc aagttctgct cggcggcggt
     3781 aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc ggctcttgtg cctgcgctac
     3841 cccataaatc cccagattga ccaacaaggc cagggaatga atcttcttgt tcattgtttt
     3901 attcctgcat ttttaccacg aattgcaact gtcgggcatg gtcgtcatca acacgacgca
     3961 tcccgttacc gcgaaaacct ttgatcctga aagacacgca gtgcagttgg ttaattaatg
     4021 tccgcgcttc ccacggcgcg ccattacgct attgtaaatg caaatggtta tcaataatat
     4081 tatcaatata tttctgcaat caatgaaaaa ttgcaaagta aacatggggt tatggtgtga
     4141 cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa acatggcccg gaatggcagc
     4201 gtctgaatga cgaaatgttt gaggtcactt tctggtggcg tgatccccaa ggttctgaag
     4261 aatactcaac gataaagcgc gtatgggtct acatcactgg tgtgaccgat catcatcaga
     4321 acagccagcc ccagtcgatg cagcgaattg caggtactga tgtctggcag tggacgacac
     4381 aactcaatgc caactggcgc ggcagctact gctttattcc caccgaacgc gatgacattt
     4441 tttctgcacc atcccccgat cgcctcgaat tacgtgaagg ctggcgaaaa ctattacccc
     4501 aggcaatagc cgatccgctg aatccacaaa gctggaaagg cgggcgaggg cacgctgttt
     4561 ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg ggattgtccg caagcgccag
     4621 aaataccagc caaagaaatt atctggaaaa gtgaacggtt gaaaaattca cgtcgtgtat
     4681 ggatttttac caccggcgat gtaacagcag aagaacgccc gctggcagtt ttgctcgatg
     4741 gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct gacttcgctg acccatcgtc
     4801 agcaacttcc tcccgccgtg tatgtgttga tcgacgctat cgacaccacg caccgcgccc
     4861 acgaactgcc gtgtaatgcg gatttctggc tggcagtaca gcaagagtta ttacccctgg
     4921 tgaaagttat tgcccctttt agcgatcgcg ccgatcgcac cgtggtcgcc gggcagagtt
     4981 ttggtgggct gtccgcgctg tatgccggac tgcactggcc tgaacgcttt ggctgtgtat
     5041 taagccagtc aggatcgtac tggtggccgc atcggggcgg gcagcaagag ggcgtgttac
     5101 ttgaaaagct aaaagctggt gaagttagcg ccgaaggtct gcgcattgtg ctggaagcgg
     5161 gtattcgcga gccgatgatc atgcgggcca atcaggcgct gtatgcgcaa ttacacccta
     5221 taaaagaatc cattttctgg cgtcaggttg acggcggaca tgatgcgctt tgttggcgcg
     5281 gtggcttgat gcaggggtta atcgacctct ggcaaccact tttccatgac aggagttgaa
     5341 tatggcattc agtaatccct tcgatgatcc acagggagcg ttttacattt tgcgcaatgc
     5401 gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta ccggcaggct gggacattgt
     5461 gtgtcagccg cagtcacaag agtcctgcca gcagtggctt gaagcccact ggcgtactct
     5521 gacaccggcg aatttcaccc agttgcagga ggcacaatga gccagcattt acctttggtc
     5581 gccgcacaac ccggcatctg gatggcagaa aaactgtcgg atttaccctc cgcctggagc
     5641 gtggcgcatt acgttgagtt aaccggagag gttgatgcgc cattactggc ccgcgcggtg
     5701 gttgccggat tagcgcaagc agatactttg cggatgcgtt ttacggaaga taacggcgaa
     5761 gtctggcaat gggtcgatga tgcgctgata ttcgaactgc cagaaattat cgacctgcga
     5821 accaatattg atccgcacgg tactgcgcag gcattaatgc tggcggattt gcaacaagat
     5881 ttgcgcgtcg atagcggtaa accactggtc tttcatcaac tgatacaggt ggcagataac
     5941 cgctggtact ggtatcagcg ttatcaccat ttactggtcg atggcttcag ttttccggcc
     6001 attacccgcc agatcgccaa catttactgc gcattgctgc gtggcgaacc aacgcctgct
     6061 tcgccgttta cgcctttcgc tgatgtagtg gaagagtacc agcaataccg cgaaagcgaa
     6121 gcctggcagc gtgatgcggc attctgggtg gaacagcgtc gccaactgcc accgcccgcg
     6181 tcactttctc cggcaccttt agcggggcgc agcgcctcgg cagatatttt gcgtctgaaa
     6241 atggaattta ccgacgggga attccgccag ctggctacgc aactttcagg tgtgcagcgt
     6301 accgatttag cccttgcgct gacggctttg tggctggggc gattgtgcaa tcgcatggac
     6361 tacgccgccg gatttatctt tatgcgtcga ctgggctcgg cggcgctgac ggctaccgga
     6421 cccgtgctca acgttttgcc gctgggtatt catattgcgg cgcaagaaac gctgccggaa
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcgcgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggatgcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagt ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggccctt attacctgct gggttgttca cttggcggta cgctggcgca gggcattgcg
     9001 gcgcggctgc gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg
     9061 ccagagacgc aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg
     9121 gagattaacc gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag
     9181 ttgtttacca ccattgaagg caactacgct gatgctgtgc gcctgctgac gactgctcat
     9241 agcgtaccgt ttgatggtaa agcgacgctg tttgttgctg aacgcacgct tcaggaaggt
     9301 atgagccccg aacgcgcttg gtcgccgtgg attgcggagc tggatatcta tcgtcaggat
     9361 tgtgcgcatg tggatattat ctctccaggg gcgtttgtaa aaattgggcc gattattcgc
     9421 gcaacgctaa acaggtaaat taatattatc tataaaccca
//
LOCUS       DADKXM010000143         9239 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.145, whole genome shotgun sequence.
ACCESSION   DADKXM010000143 DADKXM010000000
VERSION     DADKXM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.145"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..198)
                     /locus_tag="JQR05_003371"
     CDS             complement(<1..198)
                     /locus_tag="JQR05_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAY5325440.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLS"
     gene            complement(485..1210)
                     /gene="phoU"
                     /locus_tag="JQR05_003372"
     CDS             complement(485..1210)
                     /gene="phoU"
                     /locus_tag="JQR05_003372"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY5325441.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1225..1998)
                     /gene="pstB"
                     /locus_tag="JQR05_003373"
     CDS             complement(1225..1998)
                     /gene="pstB"
                     /locus_tag="JQR05_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY5325442.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2090..2980)
                     /gene="pstA"
                     /locus_tag="JQR05_003374"
     CDS             complement(2090..2980)
                     /gene="pstA"
                     /locus_tag="JQR05_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY5325443.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2980..3939)
                     /gene="pstC"
                     /locus_tag="JQR05_003375"
     CDS             complement(2980..3939)
                     /gene="pstC"
                     /locus_tag="JQR05_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY5325444.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4026..5066)
                     /gene="pstS"
                     /locus_tag="JQR05_003376"
     CDS             complement(4026..5066)
                     /gene="pstS"
                     /locus_tag="JQR05_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY5325445.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5314..6387)
                     /locus_tag="JQR05_003377"
     CDS             complement(5314..6387)
                     /locus_tag="JQR05_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5325446.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVVLAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(6398..8920)
                     /locus_tag="JQR05_003378"
     CDS             complement(6398..8920)
                     /locus_tag="JQR05_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5325447.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDMDETVCFDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(8945..>9239)
                     /locus_tag="JQR05_003379"
     CDS             complement(8945..>9239)
                     /locus_tag="JQR05_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAY5325448.1"
                     /translation="TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISV
                     MDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
BASE COUNT         2377 a         2355 c         2119 g         2388 t
ORIGIN      
        1 acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc
       61 tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc cgcgccccat
      121 gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt
      181 ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaacc cgacttcacc
      241 agtattctct ggttatgtca ggttttgcct gcgaatgcag taacaatcca gtcatttatt
      301 aatggttttt ataacgaaca tccaggttcg gaaattaatt taattgcgtg cagagaaaat
      361 agcaatgcgc tattgataaa aatatgacca tgctcgcagt tattaacttt gtgtaatttt
      421 aggaatttat aaagttatat ataacaaatc ccaataatta agttattggg atttgtctgg
      481 tgaattattt gtcgctatct tttcccgcca gcagtttatc cagctcatca ccgcctacat
      541 gacggaaatc ctgacccttc acgtagtaga agataaactc acaaatattc tggcagcggt
      601 cgccgatacg ttcgattgaa cgcgcacaga acagcgcggt aagtacgctc ggaatagtgc
      661 gcgaatcttc catcatgtag gtcatcagtt gacgaacaat accttcgtat tcctgatcga
      721 cttttttatc ttcacgataa atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt
      781 ccagcacgtc gtgcagcatc tggatggtat gacggcccag cgactccaga cttaccagca
      841 acggctgatg ctgctgggag aatttctcca gcgcagtacg gcagattttg tccgccacgt
      901 cgccaatacg ctccagctcg gcaatggttt tactgatcac cataaccagt cgcaggtcgc
      961 tcgccgtcgg ctgacgtttg gcgataatgc gcacgcaggc ttcatcgatc gccacttcca
     1021 tcatgttgac gttcttgtcg ccttcgatca cgcgcttcgc cagatcgctg tcctggttat
     1081 gcatcgcggt gattgcatca gaaagctgct gctccaccat gccgcccatg gtcatcacct
     1141 gcgtgcggat actttccagt tcggcgttga actggccgga aatatgttta ttaagattga
     1201 gactgtccat aacgcactcc tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg
     1261 tttcttcgct ggcttggtga acagatcgtc cgtgttgctg aactcaatca attcgcccaa
     1321 gtacataaat gccgtgtggt cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat
     1381 caccacggtg taatcctgct tcagttcggt gatcagctct tcaatacgcc cggtagagat
     1441 ggggtcgagc gccgaacacg gttcgtcgag cagcagcact tccgggcgaa tggcgatacc
     1501 acgcgcaata cacagacgct gttgctgacc accagagaga gagtaaccgc tctggtgcaa
     1561 tttatctttg gtttcgttcc acaatgcggc tttggtcaat gcccactgca cgcgctcgtc
     1621 catgtcggca cgggagagct tctcaaacag acgaacgcca aaggcgatgt tgtcgtagat
     1681 ggacatcggg aacggcgtcg gtttctggaa caccatgccc actttcgcac gcagcagggc
     1741 gatatcctga gagttggtca ggatgttgtc gccatcaagc agaatttcac cttccgcacg
     1801 ctgctccggg tacagttcaa acattttgtt gaaggtacgc agcagcgtcg atttaccgca
     1861 gccggacggc ccgataaacg ccgtaacctg gtttttggcg atatccaggt tgatgttttt
     1921 cagggcatgg aatttgccgt agtagaagtt caaattacga acctgaattt tactcggggc
     1981 agtttcaacc atactcattg caatcttttc ctcatcgtac ctgatgcttt tcgctaatca
     2041 ggcttaccgt agggccgggt aaggcgttca cgccgcatcc ggcaaagtgt taaccgtgtt
     2101 tattcttcgc aaaaacaacg cgcgccagaa tgttcagcag caggacgcac agggtaatga
     2161 tcaatacccc ggcccaggcc agttgttgcc attccgcaaa cgggctcatc gcaaacttaa
     2221 agatcgtcac cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg
     2281 agagcgcggt aaacagcagc ggcgcggttt caccggcaat acgggcaatc gccagcagga
     2341 taccggtcat aatcccggac accgacgctt tcagcgtaat cgcggagatc atcttccact
     2401 tcggtgtacc cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt
     2461 tctcggtggt gcggataacg atcggcacct gcaacagcgc cagcgcaatt acgcccgccc
     2521 agccggagaa gtgctccatc tgtgccacca cgatggtgta gacaaacaga ccaaccacaa
     2581 tcgacggcgc ggagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt
     2641 tacgaccata ttccgccaga taaatccccg ccataatgcc cagcggcgta ccgaataccg
     2701 tggcccacaa aattaacagc ccgctacccg ccagggcgtt cgccagacca ccaccttccg
     2761 tattaggcgg cggcgtcatt tcagtgaaca gcgccagcga cataccgtcg ataccgcgag
     2821 tgatggtgga cattaaaatc cagatcagcc agaacaggcc gaaggccatc gtcgccatcg
     2881 agagcgtcag cgcaatacgg tttttgaggc ggcgacgcgc ctgcattttg cggcgagatt
     2941 cagccagcgc cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt
     3001 agccaggcgc ataatcataa acttcgatgc ggcgaggacg ataaaggtaa tcacaaacag
     3061 gatcaggccc agttccatca gtgcggcaac gtgcagaccg gattccgctt ccgcaaattc
     3121 gttcgccagc gcagaggtga tactattgcc cggcatgtac agcgaggcgc tgtcgagctg
     3181 gtaggtgtta ccgatgataa aggtcaccgc catggtttca ccgagcgcgc ggcccagccc
     3241 cagcatgatg ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac
     3301 ttcccaggtg gtgcagccaa taccgtaggc cgactctttc atcatcaccg gggtttgttc
     3361 gaacacatca cgcattaccg ccgcaatgta cggaataatc atgatggcga ggatcacgcc
     3421 tgccgcgagg ataccgatac caaatgcggg gccagagaac agcgcgccaa caatcgggat
     3481 attcgacatg atattgccga ccggctcctg aaagtaaacg gcgaacagcg gcgcaaagat
     3541 aaacaggccc cacatgccgt aaacgatact tggaatggct gccagcagct caatggcgat
     3601 acccagcggg cgtttcagcc agccaggcgc aagctcagtc aggaacaggg cgataccgaa
     3661 actcaccggg acggcgatca gcagcgcgat aaacgaagtc accaacgtac cgtagatcgg
     3721 caccagcgcc ccgtagatat cgttcggtgc atcccactct ttggtccata ggaaagccag
     3781 accaaatttc tgaatgctcg gccaggagga gatgatcaga gagacaataa tgccacccaa
     3841 catcaatagc acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt
     3901 tttacccggt gggttaaaag cgggcttggt tgcagccata agttactctt cagttaaacg
     3961 cgtttacaaa gtcattttgt aatgccggat gcggcgtaaa acaccgtacc cggcctggag
     4021 ttttattagt acagcggctt accgctactg tctttaatat tggtcttcca cgcagcgcga
     4081 acctgttcaa ctacactatc cggcaggctg gcgtaatcca ggtcgttcgc ctgtttagcc
     4141 ccggttttgt acgcccagtc gaagaatttc agcacctctg tgccttgttc tggtttcttc
     4201 tgatctttgt ggatcagaat gaacgtggta gaggtaatag gccatgcatc ttcgcctttc
     4261 tggttggtca gatcctgagc gaaggttttg ctccagtctg caccttttgc tgcattagcg
     4321 aagttttctt cggtcggact aaccggttta ccatcagcgg agatcagttt ggtgtacgcc
     4381 aggttgttct gcttcgcgta agcatattca acataaccaa ttgcacccgg cagacgctga
     4441 acgaacgcgg cgataccgtc gttaccttta ccgcccagac cgatcggcca ttttacggta
     4501 gagccagtac caacgttgtt tttccactct tcgttcactt tcgccaggta gctggtgaag
     4561 acgaaggaag tcccggagcc atctgcgcgg cgtactacag caatgttttg tgaaggcagt
     4621 ttcagacccg gattcagttt ggcgatggct tcatcatccc acttcttgat tttgcccagg
     4681 tagatgtcgc cgagggtttt accatccagc accagttcgc cagacttcag ccctggaatg
     4741 ttaaccgcca gcaccacgcc gccaatcacg gtcgggaact ggaacagacc ttcctgagcc
     4801 agtttttcgt cagacagcgg cgcgtcagag gcaccaaaat caacggtatt agcgataatc
     4861 tgttttacgc caccggaaga accgataccc tggtagttaa ctttattacc ggtttctttc
     4921 tggtaagtgt cagcccattt ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc
     4981 aggcttgctt ctgcaaacac agagaaagca ctcatcgata aggtcgcggc gacaacagtt
     5041 gcgacggtgg tacgcataac tttcataatg tctcctggga ggattcataa agcattgttt
     5101 gttggctacg agaagcaaaa taggacaaac aggtgacagt tatatataag gaatatgaca
     5161 tttttatgac agaaaggaat taccaaataa cttactgatt ttaaatgtga agatgaaaat
     5221 cccacatgcc atctttttgt catgatgcat ttaaaagtga taaagaaatg ggtgagatga
     5281 caacgatgtg atgaacaatc gttgtcagac ctattagcgc acaatctcaa cagttatcgt
     5341 cgctgttgca gtaaattgcc ccggggctgg gcgagctcca gtaaggctcg caggagcagc
     5401 tgaaaaagtt acactacctg atttttcacc atacactgtt gatttaccca tgtctgttgg
     5461 caactcacca ccgttcactt tgatttcctt attatttttg tcgtaaattt ttatccccac
     5521 gttagagttt gaagttacga ccattgaatt gtccgtattg ggttcggtag cataaaaaga
     5581 cgtactgata tcatcggtat tcagtatccc caggttttca cactcaatag tgactgtttt
     5641 agtaatttta tgtgttgtta ccgcacttcc tacaacagat gaaaaatctg cagctggtat
     5701 ttctttaaaa tcaaaacaaa ctggctttaa attatttatt tcgcaacttt gcggagctgt
     5761 aatatcgccc cgaatataaa catcagccat cggctgtgag taatccaccg gggtgtcacg
     5821 gctaattgtt ccatacaatg tcactatctt tgtttcagga ataatcaatt ttcctgcaaa
     5881 tgtcttcttg atataaaaat atactatcgc tttagcccca ctggatatac ttaaaggttc
     5941 aatgcggttg caattgtact ttgtattacc agacgggttg ttagcatgat attcaaaagg
     6001 tgcattcaca tacccaaccc caagaatatc tatagcaatc ccaacatcaa ggttcggatt
     6061 aagatcataa taattaatat tagagtgaac cccagcataa gttaatgcct ggtttatttt
     6121 tgacgtataa tagatatagg ggaattctcc tacgttacca gtatcacaat gacagcgtcc
     6181 aagatatgag gatccattag ttacagtggt gttaaatgtc gttccaacct tattttcgtt
     6241 cgcggtaata agatatcccg agaaatcaag cgtactgtga aatacgccat tatcaggggt
     6301 acagatacct ttaggagctg caagtactac actacttaac gagagcataa acacagcaaa
     6361 gcaccactgt ttgatatact tgttcataaa aatcctttta atgacatgtc acaccctgtt
     6421 caattaatcc gctctcactt tctggtgtcg aaagtgaata attgaagatg cattttgttg
     6481 aggcgttttc tccccaggat aacgtaacct gtcctttttc tggtagacca gaaagataaa
     6541 gttcgccacg gtcaccgaca attcctgaat ctgccaaact tgcatcttgt atcgcagcaa
     6601 tggctccaaa aggtaccggt ttaccatttg catttaacac tctgaagaga acgcgataac
     6661 ccacatgagt cacaaactcc gctttaacca ctgcaccacg cgtgggaacc atcgtaacga
     6721 cggtattatc aagttctata ttcgacttcg agaaatagtt atcgctgatt gctacccgat
     6781 tttcatcata agggtttagc tgtgtcttga ccgtatagcc gcgccagtcc gtcgcagcac
     6841 cgcgcatatt atctatttct aacccggcgg ccccaggtgc tttaacaaga gcaatagttt
     6901 cacctaactc ctgacttaac gttacccctt cgctgtgtgc cagaattccc cctctgatac
     6961 cataattcag cctctgggaa ttttggctgt agttatagct gccgttcacc gtgccataag
     7021 agccatcata ggtcagggac atattaccgc caacctcatc atcatgactg ttataggact
     7081 gcattaaatt ccatgaaagg ttatttcttt ccagcgccgt accgctgagt ccgagttgat
     7141 gtgagctacc ggaattatcg ttttgcgaaa cagaataggt ggcgtaacta ttaccccacc
     7201 agcccgataa aggaacagat aaagagagca tgtagatctg ctcactggtg ttatcactgg
     7261 catggttgtt ccccgtcccc tgtctggcag tattggaata actccaggag acgttccacg
     7321 agagacgctt ccagctactg ctgtagccaa attgcattaa tcgttcgaca tcatcggtat
     7381 gccagtattg ttgccggctt aatgtcaggt ataaattgcc ataatctctc cccaacgact
     7441 gcgttagcgt tccctccacg cgactacgtc ggttacctgt ttcccaaaag tcttcagggc
     7501 tgttgcgccg cgatgcccac tcattgaggg tatagtagcc ctcggtggag taacgatagc
     7561 caaccaggcg caagctagtg tctgtgtcgt caaatagttt tgaatagaga aagcgatatg
     7621 actgacccgt ttctgtatca tcatggctaa aattcgcacg agcatgtgtc acatcgaaag
     7681 aaattgcccc caaagcgcca agatctttac cgacacccag agctgcagaa ccataatttt
     7741 cagcagcctg catacctcca tataaagtga tcccgtggga aaaaccatga agtatttgcg
     7801 cctgaaggac gtccggtgta aatccgtctt catcgcgcaa ttcacccacg ctgacatcaa
     7861 catctgtctg accttcacgt ttaagaatcg ccaatgaagc ataaggttgg gtgaatcgtt
     7921 gttcgctacc gtcttcttct ttgatggtta cgtccaaatc gccacctgta cttgcggtgt
     7981 tgagatcggt gatctcaaaa gcaccttgtg gaacggctga ctgataaatc acgtagccat
     8041 tctgccgaat gatgacggtg gcgttggact ttgcaatccc acgaaccaca ggagcaaagc
     8101 cattctggct ggcgggtagc atatcgttat cagtatacaa acgcgcgccg cgaatttgcg
     8161 tactgtcgaa aatatcgctc gccgtccacg tatcaccaat cataatctgg cttcttaacg
     8221 cggcaatatc gcgctgtatc caggtactaa tggagttgta ttccgcacgg ccatcgctgg
     8281 tgtttaaagt actgtaatta cgtagccgcc acggtccaat attcagtcca ttgcgcagat
     8341 tgagataact ggtatctgtc tcttgtgaac cataatctga acgtagtgta tgtgaaccag
     8401 aaaaatcgta gttgatggtg agcgcattaa tcccctcatc ccaacgcgtg gggctgatgt
     8461 aatcataagc catcgcgtcc atgtagattt ggtgaatgga tatattcagc tgcaatttat
     8521 cactgtcaaa ctgccaggat gagccttcaa tacgtggagc aggatcgaag caaaccgtct
     8581 catccatatc attgaacgat ttgagcgcat caacttttat gccaaaggag gacagtgttt
     8641 ttttaatggc aataaagcaa gcgtgcagat cagcatctgg attatcctga acaaatgtca
     8701 catcctgacg ttcaaacatt ttaccgttga taaaaatatc aacgctataa acacctgggg
     8761 gcatcaggtt tgcccgatta aactggcgaa tatcgactcc ggattgatgg tcgatttcca
     8821 gaagcgccgg attgaataca ctgacctcgg ccatcaggtt ttgcgagaaa atcagacacg
     8881 tccctgccgt gaggccaacc actattctgg tcgtcatcat aatacgccta tccttatccc
     8941 ttgcttacag cgtcattttt atcgggtcac tgactgcgcc gtaatcatta ataaatcgcc
     9001 aggtcatttg gcctcccgca gaattagcag gtaaaggtaa cgtgagcgtt tcaaaaggac
     9061 ggatcatatt gaggggcaca tcatggccat caacgatgac atccatcaca ctgatatgaa
     9121 ttgggttggg attctctgct atcagatttc gcccttcccg gcgccatttc actttcggcg
     9181 cagcatcttc gggaataagg cggatatttg ctggtcgcca gaacagcttg atacgcgtt
//
LOCUS       DADKXM010000144         9013 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.146, whole genome shotgun sequence.
ACCESSION   DADKXM010000144 DADKXM010000000
VERSION     DADKXM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.146"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..373)
                     /locus_tag="JQR05_003380"
     CDS             complement(<1..373)
                     /locus_tag="JQR05_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY5325449.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEG"
     gene            complement(570..1370)
                     /locus_tag="JQR05_003381"
     CDS             complement(570..1370)
                     /locus_tag="JQR05_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY5325450.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIAHDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTVRTTGRAEATIL
                     VDIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(1642..5544)
                     /gene="hrpA"
                     /locus_tag="JQR05_003382"
     CDS             complement(1642..5544)
                     /gene="hrpA"
                     /locus_tag="JQR05_003382"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY5325451.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5745..6350
                     /gene="azoR"
                     /locus_tag="JQR05_003383"
     CDS             5745..6350
                     /gene="azoR"
                     /locus_tag="JQR05_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY5325452.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(6404..7714)
                     /locus_tag="JQR05_003384"
     CDS             complement(6404..7714)
                     /locus_tag="JQR05_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY5325453.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWSHFRQHLAVR
                     WRKVCGGWFLLIAISTLATWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALPYVVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKL
                     PPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEF
                     PRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALV
                     VAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(7704..>9013)
                     /locus_tag="JQR05_003385"
     CDS             complement(7704..>9013)
                     /locus_tag="JQR05_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY5325454.1"
                     /translation="ARPALALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITR
                     AIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKEL
                     HLLPGFYHDTLGEENRAQAFEKMQSFICRLYANKSQKFDYQHEDRTGPSADRWRLLSG
                     GPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFG
                     RLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDAL
                     ANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLA
                     IVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKP
                     WVMRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT         2211 a         2434 c         2184 g         2184 t
ORIGIN      
        1 cgccctcgta acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca
       61 cttcgacttc agccagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt
      121 cactggcgcg ttcgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag
      181 caggcaacgc ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat
      241 cctcggcctg accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat
      301 caatccatgc gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa
      361 cgggtactga catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt
      421 aattaacatc aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca
      481 taaaaacaag tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg
      541 caaacaagtg gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg
      601 atgagcaccg catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca
      661 acgtgaacga taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc
      721 ggcagctcgc cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga
      781 ttaataaaga ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt
      841 aaccagcgtg gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc
      901 cgctgcatgg tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc
      961 aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag
     1021 attttttcat gcggaatatg ccagaactga tgagcgatat ccaccaggat ggtcgcttct
     1081 gctctgccag tggtgcggac agtgttgtag tgcggatgct gtgcgatggc gctatacaaa
     1141 aaagtggtcg agtgaccgat accaccacta atcagtaaag gaatttgctg atcgtgagca
     1201 atcttacatg ccgcatcgat agtcggcata accgcatttc ctgcaagtat gacgcaatca
     1261 gcctgatacg gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt
     1321 atggcatcta tagttgccgt cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct
     1381 tatcgttccc ttaagaatac tgcgtgagat aatagtgaga agcggagcac tctgaaagga
     1441 ggaagtcgta tttccttaac cgccactccg caagcctgac atatctttta aggaaatgct
     1501 gtagataaac attattcatc ttgcagctga gaattgtatt tttctgaatt cctgaatttt
     1561 tgtccttacc gttttagaat taataaaaaa cccggcgcta aatgcttacc gggttttaga
     1621 tttattaggc aaatagcagg gttaaccgct aatctgctcc atcgcctgca aaatacgctt
     1681 atctgaaatc ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc
     1741 tatcatccaa cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt
     1801 gatccattgc tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg
     1861 atgtggatca accgccagtt tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt
     1921 gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat
     1981 ctgcgcttta atgtcagaaa gccccagtgc catggtcata tccacccgcc ctttcagacg
     2041 tttgttgata ttgaacaccg cagtgaggat ttgctcgacc tgcttcgcaa tatccaccac
     2101 cgtgtcattc agttcggcgc gcactttttc atgcagcgca gcaaagcctt cttccgtcca
     2161 gaccgggcca ccattggcgt cgatcagttg atccacaccg caggagatac agtcgtcgat
     2221 cagctccagc actttgccat acgggttaaa gtacagcccc agcttggctt tgtttggtaa
     2281 cttctcatgt aaatacttga tcggcgagga aatatttagc agcagtagac ggcgaagacc
     2341 attccacatt gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacgctgtc
     2401 gcgttcatcc accagcgccg gccacgcctt cactttatag ttgccacgct tctgttcgta
     2461 actttccggc agctgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc
     2521 cgccaccgcg gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag
     2581 cgagcgccct tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag
     2641 gtgatcgggc acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg
     2701 ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa
     2761 aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga
     2821 tttaatcaga gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct
     2881 ttcctcaacc tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtccgc
     2941 gcctggctca aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg
     3001 gtaatccagc ttgctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt
     3061 gagcaaatca ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga
     3121 gattacatcg tggctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag
     3181 aatatcgcgg cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt
     3241 ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc accagagcgt ggcgaataaa
     3301 gagttcacga cataacgccg aatcgatctg gctgtagttg accttgcgcg cagcaacaat
     3361 cggcaaacca taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca
     3421 gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca acgggttcta cccattccgg
     3481 gtcgatacgc gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat
     3541 tacccatttc ggcggctttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt
     3601 acgtgcaccg gtatattcct gtttatcggc atctttcatg ccgatatggg aaagcaaacc
     3661 ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt tcgctgttga ccggaatgcc
     3721 aagttctttc accacctggc gcaactgggt gtagatatcc tgccattcgc gcacgcgcag
     3781 atagttgagg taatcggtac gacacaggcg acggaaggcg ttggaagaaa gcgccttttg
     3841 ctgctcgcca agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg
     3901 gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc atcggacgtt cgcgcggatc
     3961 ctgaatggag agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttttgcgc
     4021 ttccagcacc atacgcgcca gacgtgggtc gacaggcaac tgcgagagct ggcgaccgag
     4081 cggcgtcagt ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag
     4141 cagacgcacg ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc
     4201 gatatcgccc agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag
     4261 aatctccgga tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat
     4321 acagatccct tcggacacac gaccacagcg gcctttacgc tgattggcag acgcctgcga
     4381 aatgggttca atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc
     4441 tgtaccgggg tcgatgacgt atttaatccc cggtacggtc agcgaggttt ccgcgacgtt
     4501 ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac acgcggttct gttcgctgtt
     4561 cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc
     4621 atcggcggta tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccggggct
     4681 ttcctggctt agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc
     4741 atcggcttct tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac
     4801 ttcaataatc ggcgcattat taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga
     4861 agtgataatg atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag
     4921 aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata atgatggtgt cgtactgcat
     4981 cagcaggcgg tcttgctgga tctccgccag caggatcccg tcggtcatca gcttgaccat
     5041 cgtgttatca cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc
     5101 cgttttcagc tcttccgcaa tacggttggc caccgttcgt gccgccaggc gacgcggctg
     5161 ggtatgaccg atcagccctt taataccgcg ccccagctcc atacagattt tcggtagctg
     5221 agtcgtttta ccggagccgg tttccccggc gacgatcacc acctggtgat cacgaatcgc
     5281 ttcgagtatg tcctgttttt tctgactaac cggtaaattg tcaggataag tgatttccgg
     5341 tcgtgccgct tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc
     5401 ctggaaaatg gcctgttgtg catcaggatt tttaaccttc ttaacacaat gcagacggcg
     5461 agaaaaacgc agtctgtcgc gcagcatcag cgaatccagc cgctgttgca aggcagtaaa
     5521 ggtcaatttt tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc
     5581 ttttaaatga tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc
     5641 gaacatagac ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcagc
     5701 aagcaacggg gcatcgtcca ttcaaacatc tataaggaaa caccatgagc aaggtattag
     5761 ttcttaaatc cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg
     5821 ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctagctgcaa
     5881 atccgattcc ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc
     5941 tgactccgcg tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag
     6001 cccacgacgt tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga
     6061 aaaactattt tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc
     6121 cggaaggtct ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca
     6181 aagatggacc aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca
     6241 ttaccgatgt gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga
     6301 aagcacagtc tgacgcaaaa gcagccatcg acagcattgt tgctgcataa ccggacacgg
     6361 tcttgatgtg gactgtagat attcagtcca catctcaatc cacttacctg ttttcccata
     6421 atttcaacat cgctttgtgc tcttccttaa gcacaatatg cgagcgtctg gctcgaataa
     6481 agctaatcgc ttcatcaact gttttacagt gtccgtaaca taacagccat gccgccacta
     6541 ccagcgcact gcgcgataat cccaatgcgc aatggaccag aacgctgcct tgctcttcgc
     6601 gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc ctcttccgga accaccagat
     6661 ccaacatcgg tacacagaaa tagagtcgat cttttgtcgc tcgtccacga gggaattcaa
     6721 aggtgacgtc cagaaccgca ttctgtgccg gaatatgtcg tggaaacgcc cttaaataaa
     6781 caccagcagt cattttgctc accggctcca ggcgacgaca aaaccaacgc atagatagcc
     6841 acatcccgat gcgccagggc aatgtcagcc agtaaacggc gggcgggagt ttcccctgac
     6901 tatctttccc tgttgttatc gcgccaagcc caccgtaccc acggccaata atgagtagcg
     6961 ataatactgg ccaacataac cagactgacc accataactg aaccatcatc attagctcca
     7021 tcaacacaat gcacgcgcct acgacatatg gcagtgctat tttgattcga cgttgatcaa
     7081 gtttctgata attccaacga cggtcgacgg gcaccatcca gtcaatcaac atacctaccg
     7141 ccagccctgt gatgacatca ataaaatgat gctgccatgt cgccagcgtc gaaatggcga
     7201 tgagtaaaaa ccatccgcca cagactttac gccacctcac agccagatgc tgacgaaagt
     7261 gactccaaag tagccagcag agaataatat gcagcgaagg agactggtta taaggcagat
     7321 caaacagttc aagttgcgaa aatagccatc ctgtcacccc actcacttca ggacggataa
     7381 aactaaactt cagcgggtag agaaaaaaac cgcagcaggc cattaccgtt gccagaataa
     7441 gccggtggac aagtcggcgt tgttcgaatg tcgagctaca aacgaacagc gaaaatccat
     7501 ataaaagatc cagactccag taaggaacaa tagtccaggg aagaaaaggg atcgccgttt
     7561 cccaaccgaa tacctgactg gggatatcat ggctgttaag gtcctgaacc gcggtgaact
     7621 gattaagaga tccataggtg aagaagaaaa acggggccag caacaataac cagccagcgc
     7681 cttgtagcaa tacgttccga cgttcagtta tcacgacgca ccgccatcga aaccgtaaaa
     7741 atgccccaca catcaatccg ttgagtgcat ttatcaaatc cggcattacg cacgagcgaa
     7801 tccatctccc cttgcgaacg cacgcgcatt acccacggtt taccatcttt atgactggtt
     7861 aacaccccgg caatcagctc cagttgtggg tgccacggct gcccggtgta gatgagaatg
     7921 ccacccggtt cgatggcatt ggctagacct gcgagtgagt tttttacctg ctcattttcg
     7981 ggaaaaagct catataggcc agagacaatc gccagcgtag gccgcggagt taacgcgctg
     8041 agttcctccg ggttaaacgc atcgccctgt tcaaaacgca cccgcccaga cattccccgt
     8101 tgggcaatca tctcttgccc ctgtgcaaca tttaactcac tgtaatcacg taacaaaata
     8161 tcgcttacgg caggctcgtt tgccagcgca tccagtacat agcgtccatg ccctgcggca
     8221 atgtcaacca cgcggacggc taaacctttg gcgtggagat cggcaacggc ttgtttaatc
     8281 aatatttgca aatgggtttt gcgctggcga ataccgcgcc atccaacact gttcaggtag
     8341 attttgtcga ttaaacgccc gaatgcgtta ctgccttgcg gttgattttg atagacataa
     8401 tccagggaac tgcctgaatc aaagccggta ctcattccga gatgcaggcc cgcagagtgc
     8461 gcaccgaaca atttcatcgc ctttcgcata aagcgatacg ctaaatcaac cggcgataat
     8521 ggcacgggtc caccagaaag gagccgccat cgatccgctg atggtcctgt gcggtcttca
     8581 tgctgataat caaatttttg cgatttgtta gcatataagc ggcagataaa gctttgcatt
     8641 ttttcaaatg cctgcgccct gttctcttca cccaatgtgt cgtgataaaa gcctggcagc
     8701 agatgcagct ctttcagagg gctacgtaat ctctgataaa aatcaatctg cggttggcga
     8761 tgcaccacat agtcatcacc tgatatcaga agttgcgtgg ggagcgtaat cgccgccgca
     8821 tcgctaacaa tacgttcaga cgttttatag agatcgagca agatattgac ggcaatcgcc
     8881 cgtgtgatca gcggatcatt attgaaactc gccacccgtt gccgatcgtg ggtcaaatag
     8941 cgccctttca cataggaatt aataaaaaat agaccacgca gacgatgcca taacgccagc
     9001 gcaggacgtg cca
//
LOCUS       DADKXM010000145         8972 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.147, whole genome shotgun sequence.
ACCESSION   DADKXM010000145 DADKXM010000000
VERSION     DADKXM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.147"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            465..3332
                     /locus_tag="JQR05_003386"
     CDS             465..3332
                     /locus_tag="JQR05_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5325455.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSHSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3432..5360)
                     /gene="dhaR"
                     /locus_tag="JQR05_003387"
     CDS             complement(3432..5360)
                     /gene="dhaR"
                     /locus_tag="JQR05_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY5325456.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5579..6649
                     /gene="dhaK"
                     /locus_tag="JQR05_003388"
     CDS             5579..6649
                     /gene="dhaK"
                     /locus_tag="JQR05_003388"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY5325457.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYITRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6660..7292
                     /gene="dhaL"
                     /locus_tag="JQR05_003389"
     CDS             6660..7292
                     /gene="dhaL"
                     /locus_tag="JQR05_003389"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY5325458.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7303..8721
                     /gene="dhaM"
                     /locus_tag="JQR05_003390"
     CDS             7303..8721
                     /gene="dhaM"
                     /locus_tag="JQR05_003390"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY5325459.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         2352 a         2223 c         2204 g         2193 t
ORIGIN      
        1 tagcacaata ataacgatag gccagaaaat ccattgacca aaaatgaata tcgatgtttt
       61 ttgctttttc ttattagtaa tgcaaaaatg gcgcatattg tttttgttat gcagatacat
      121 caagcgaaaa tcccataact aattaggatt attacaaaaa atcttcacca aattttttaa
      181 ccgctgtttg ttgtaatatt tttgccatat tcatagtaac gaattggaat aagtaacgac
      241 aacttaggga ataactcgat ataaactcac ctttgtaagg ggggtgttaa atatatatta
      301 attcctgtgt tcctgatttt gcccggtaat agagaattat tctcagagaa gctcatcatg
      361 attgcaccat acagatatca tgatgataag aggatattat tcactgactt aaacaaaatg
      421 acaaaatcca tagacccata gcctgggtac tatataggag tggtatgggc atcaaacaac
      481 acaatgggaa taccaaagcc gatcgtctcg ctgaattaaa aatccgttcg cactcaattc
      541 aactgataaa atttggcgct attggtttga atgcaattat cttttccccc ctgctgatag
      601 ctgctgatac aggaagtcaa tatggcacca atattactat taatgatggt gacagaatta
      661 caggagatac cgccgatcca tcaggaaacc tctatggtgt aatgacccca gcaggaaaca
      721 cgcctggcaa tatcaacctg ggtaatgatg tcaccgtcaa tgtcaacgac gcctctggat
      781 atgcaaaagg aatcattatt cagggcaaaa acagctccct gacagctaac cgactcacag
      841 tagatgttgt tggtcaaacc tctgccatcg gcattaactt aattggtgac tatacccatg
      901 ctgacttagg cacaggcagc accattaaga gtaacgatga cggcatcatt attgggcata
      961 gctcaacact aacagccact caattcacca ttgaaaactc gaacggtata ggcctaacca
     1021 tcaatgacta tggcaccagt gtcgatcttg gaagcggaag taaaatcaag accgatggaa
     1081 gtacaggtgt ttatatcggt ggtctcaacg gcaataacgc caatggtgct gcgcgtttta
     1141 cggcgacaga cctgacaatc gatgttcagg gctacagcgc catggggata aacgtacaga
     1201 aaaactctgt tgtcgatctc ggaacaaaca gtaccattaa aaccaatggc gataatgctc
     1261 acggcctctg gagctttggc caggttagcg cgaatgcact cactgttgat gtaactggag
     1321 ccgcggccaa tggcgtcgaa gttcgtggtg gtacaaccac tatcggtgca gatagccata
     1381 tttcttccgc gcagggcggt ggcctcgtca ccagtggttc agacgcgaca atcaattttt
     1441 ctggcacggc agcgcaacga aacagcatct tttccggcgg ttcttatggt gcctcggccc
     1501 agacggcaac ggctgttatc aacatgcaaa ataccgatat tacggttgat cgtaatggca
     1561 gtctggcgct gggtttgtgg gcgctcagcg gcggtagaat aaccggagac agtttggcta
     1621 tcaccggcgc ggcaggagcc agaggtattt atgccatgac caacagccag atcgacctca
     1681 cgagcgatct ggtcattgat atgagtacac ccgaccagat ggccatcgca acgcaacatg
     1741 acgatggtta tgccgccagc cgcatcaacg cctcgggtcg tatgcttatc aacggtagcg
     1801 ttctttccaa aggtgggcta atcaatctgg atatgcaccc tgggtcggtt tggacaggtt
     1861 cctccctcag cgataatgtc aatggcggga aactggacgt tgcaatgaat aacagcgtct
     1921 ggaacgtaac aagtaattct aatctcgaca cgctggcgct gagccattca actgtcgatt
     1981 ttgccagcca cgggtcaact gccggcacat ttaccacatt aaacgtagag aacctgagcg
     2041 gtaacagtac ctttattatg cgtgctgatg ttgttggcga gggtaatggc gttaataata
     2101 gaggggattt attgaatatc agcgggagta gtgctggtaa tcacgtattg gctatccgca
     2161 accagggcag cgaggccaca acgggaaatg aagttctgac agtggtaaaa accactgacg
     2221 gcgcggcctc gttcagcgcg tcttctcagg ttgagttggg gggatatctg tacgatgtgc
     2281 gtaaaaatgg cactaactgg gagctttacg cttccgggac agttccggaa ccgactccta
     2341 atcctgaacc cacaccagct cccgctcagc ctcccatagt caaccccgat cctacgcctg
     2401 aacccgctcc cacgcctaaa cccaccacga ccgcagatgc tggcggcaat tatctcaatg
     2461 tcggttactt attgaactat gttgaaaacc gtacgctgat gcaacggatg ggtgacttgc
     2521 gaaatcagag taaagacggt aatatctggt tgcgcagtta tgggggaagc ttggactcct
     2581 ttgccagtgg caaactgagc ggctttgaca tgggttacag cggtatccag tttggtgggg
     2641 ataaacgtct ctctgatgta atgccgttgt atgtcggtct gtatattggc tcaacacatg
     2701 catcgccgga ctatagcgga ggcgacggta ccgcacgttc agactacatg ggaatgtacg
     2761 ccagttacat ggcacaaaac ggtttttaca gcgatctcgt tataaaagca tcgcgccaga
     2821 aaaatagttt ccacgtactg gacagtcaga acaacggcgt taacgccaac ggcactgcga
     2881 atggaatgag catctccctg gaagccgggc agaggttcaa cctgtcccct actggttatg
     2941 ggttctatat agagccgcaa acccagctta catacagcca ccagaatgag atggctatga
     3001 aggcgagtaa tggcctcaat atacatctga atcactacga atcgctgctg gggcgtgcca
     3061 gcatgatact ggggtatgac atcaccgcag gcaacagcca gctgaatgtc tatgtgaaga
     3121 ctggcgctat ccgcgagttt tcaggggata ccgaatatct gttgaacgac tcccgggaga
     3181 agtacagttt caaaggtaat ggctggaata acggcgtggg agtcagtgca cagtataaca
     3241 aacagcacac attctatctc gaagcggatt acacgcaggg taacctcttt gatcagaagc
     3301 aagtcaacgg aggatatcgc ttcagctttt aagtgcggga tagacaagcc cttaaacgat
     3361 gctgaaaatt gttagcgtgc gccggaatga aatacagatc actctgatgt atcagaaatc
     3421 gtttctgtct ttcatacccg gcgcttaaac tgccctgcat caatgccatg ttgcttcatt
     3481 ttccgccaca gcgtagtgcg gccgatccca agtaaagccg acatttcctg aatgcgaccg
     3541 cctgtgacct gggctgcgtt aataattgcc tctttttcaa cttccgcaaa tgacagactg
     3601 gtggaaaggc gggtggcgct gacatcatct gtcgcctgct cggtaaacag atgttcaggt
     3661 aaatcactga cgcgaatgcg cccgttatca ctactcagag caagattctc gatgacgctg
     3721 taaagttcaa aatcgttgcc tggccatgca caagaaacca ggcgagcgag ggcatcgtca
     3781 tcaattttca gccgcgtaga gaagcgtttt tcaagactgc gtaatttgtt attcaccagc
     3841 gccggaatgc tgccacgccg catacgcaga ggcgggatgg taatttcaaa tgcatgcagc
     3901 gcgtaataca gctggcgact aaaacgattt tgttccacca gcattgcgag gtccgcggtc
     3961 gttgttgcaa tcactttgac atcaattggt attaaacgcc gcgcatccag tcgcgtgata
     4021 accccctgct tgataacctg aagcaaagca gactgtaact ccaccgccag atattcaatc
     4081 ttttcaagaa acagcgtgcc gccgtgtgcc agttccagcc gactcagacg gccattttca
     4141 ttgtccgtgc gatcgccacc aataaattct tccgccagcg cagcatcacc atataactca
     4201 caattgacgg cgatataagg acctgcagca cgctcgcttt cattatgaat tgcctgactt
     4261 agcagtgcct tgcccacgcc ctcttctcca caaagcagga caggaaagct actgcgcgcc
     4321 gcctggcgac caaaatgaat caagcggcgg gtttgcggat cgtcctgtgg catatgagcg
     4381 aaggtatggc tgacttttcc taattgactg gtcattaact gccgcatctg ttccacagga
     4441 tggagcaaca aaataaagct ggttccctgc gtttctatta tcggtttaag ggttatcacc
     4501 gcatcaataa actggtgctg gctttcaaag gttgcttcta cgtgtttgag cggatgtgcc
     4561 tgttttattg cttgttgcaa tacggcgggt aacgttaaga gttcagtgat tgcccgtccc
     4621 tgacttgccg tcgcgtcaag gcgcaagacc cgcgccgcct gggcattaat aaattgcaaa
     4681 ttaccctgct cgtcccagct aatcacgcca tcatccatac tttctaacag ggcattaagt
     4741 tgatttaaat gacggttagt ttcagcgagc aaactgtccg tcagcagtaa attaccgacc
     4801 tcgcgggcga ttgccaacgt caacggcaaa tcagctgcgg tagtttgctc aaccggacac
     4861 gccagcgcta ttgttcccgt caatcggccc ttgctgtcaa acaacggcgt tgcacaaaag
     4921 gcccagttcc agagtgcctg tttgaaatgt tgatcggcca tcgttttcac ggcctgacca
     4981 gagatagccg ctaacgatag cgcacaagta ccaataattc cctcggcgca atacgtgccg
     5041 tcattgaatc ccagtgcact tagctgctgc aaggtttgcg gatcgccatt acggctgaga
     5101 atgcaggcgg tttcatcgag gataaacagc gcacactctc gcggtgccat atattcccag
     5161 gcatcttcca gcgcagcctg cccgagcgtc agcatcgctt tcttacgccg ataaatagaa
     5221 gcaaatgtca cgccctgggc ctgatgtggt acgttccatg tctcccgttt catcagctta
     5281 ttgcatcgct cccaggaggt tgcaattaag ggagatatgc cccgaccatc gttgttaaaa
     5341 gcgccactca tatcccgcat cccttatgac aaaaggtgtt ccggctcgca attttaagta
     5401 ttaatacacg taaaagtttc aatatggaac attcattaaa gcatatgttt cactatgaaa
     5461 cataatttgg gctaattttc ttttcccttg cctgatgcac aatggattca tcggcagtca
     5521 gtggtcgccg tgtcgttgaa catcatccat gccctaccgt aattgctgga gcaaaataat
     5581 gaaaaaattg atcaatgatg tgcaagacgt actggacgaa caactggcag gactggcgaa
     5641 agcgcatcca tcgctgacac tgcatcagga tccggtgtat atcacccgag ctgatgcccc
     5701 tgttgcagga aaagtcgccc tgctgtcggg tggcggcagc ggacacgagc cgatgcactg
     5761 tggttatatc ggtcagggga tgctttcggg ggcctgtccg ggcgaaattt tcacctcacc
     5821 gacgcccgat aaaatctttg aatgctccat gcaaattgat ggcggcgaag gtgtactgtt
     5881 gattatcaaa aattacaccg gcgatattct taactttgaa acagcgaccg agttactgca
     5941 cgatagcggc gtaaaagtga ccactgtggt cattgatgac gacgttgcgg taaaagacag
     6001 tctttatacc gccgggcgac gcggcgttgc caacaccgta ttaattgaaa aactcgtagg
     6061 cgcagcggca gagcgtggcg actcactgga cgcctgtgcg gaactggggc gtaagttgaa
     6121 taatcaaggc cactcaatag gtatcgctct cggtgcctgt accgttcctg ccgcgggcaa
     6181 accttctttt acccttgcgg ataatgagat ggagtttggc gtcggcattc atggtgagcc
     6241 aggtattgac cgccgcccct tctcttccct tgatcaaacc gtcgatgaaa tgttcgacac
     6301 cctgctggaa catggctcat accatcgcac tttgcgtttc tgggattatc aacaaggcag
     6361 ctggcaggaa gaaccacaaa ccaaacaacc gctccagtct ggcgatcggg tgattgcact
     6421 ggttaacaat cttggcgcaa ctccgctttc tgagctgtac ggcgtctata accgcctgac
     6481 cacacgttgc cagcaagcgg gattgactat cgaacgtaat ttaattggcg cgtactgcac
     6541 ctcactggat atgaccggtt tctcaatcac cttactgaaa gttgatgacg aaacgctggc
     6601 tctctgggac gccccggtcc acaccccggc ccttaactgg ggtaaataag gagaaagcaa
     6661 tgtcactgag cagaactcaa attgttaact ggctcactcg ttgtggcgat attttcaaca
     6721 ccgagagcga gtatcttacc gggctggatc gcgagattgg cgacgctgac cacgggctaa
     6781 atatgaaccg aggctttagc aaagtggtgg aaaaactccc tgctatcgca gataaagata
     6841 tcggtttcat tctcaaaaat accggtatga cgctgctttc cagcgtcggt ggtgccagtg
     6901 gtccgctgtt cggtaccttc tttatccgcg ccgcacaggc gacccaggca cggcaaagcc
     6961 tgacactgga agagctttat cagatgttcc gcgatggcgc ggacggcgta atcagtcgcg
     7021 ggaaagccga acctggcgat aaaaccatgt gtgatgtgtg ggtgccggtg gtggaatcgt
     7081 tacgtcagtc cagcgagcaa aatctctctg ttccggtggc gctcgaagct gccagtagca
     7141 tcgccgaatc cgctgcacaa agtacgatta cgatgcaagc ccgcaaaggc cgcgccagtt
     7201 atctcggtga acgcagtatt ggtcaccagg atcccggcgc gacctcggtg atgtttatga
     7261 tgcaaatgtt ggcgttagcc gcaaaagagt aaggaattgg tgatggtaaa cctggtcata
     7321 gtttcacata gcagccgact gggagaaggt gtcggtgaat tagcccgtca gatgttaatg
     7381 agtgatagtt gtaaaatcgc cattgccgcg ggaattgacg atccacaaaa tcccattggt
     7441 accgatgccg tcaaagtgat ggaggccatc gaatctgttg ctgatgccga ccatgtgctg
     7501 gtcatgatgg atatgggtag cgcattattg agtgctgaaa ctgcgctgga attgctggct
     7561 cccgagatcg ccgcaaaagt acgtttgtgt gctgcgccgt tggtcgaagg tacactggca
     7621 gcaacggtca gcgcggcctc gggggcggat atcgacaaag ttatctttga cgctatgcat
     7681 gcgctggaag ccaaacgtga acaactgggt ttaccgtcct ccgacactga aatctctgac
     7741 acatgtcctc cgtacgatga agaagcccgt tctctggcgg tggtcataaa aaaccgtaac
     7801 ggcctgcatg tacgtccggc ctcccggctg gtttatacct tatcgacatt taatgccgat
     7861 atgttgctgg aaaaaaacgg caaatgcgtc acaccagaga gtattaacca gattgcgtta
     7921 ctacaagttc gctataacga tacgctgcgc ctgattgcga aagggccaga agctgaagag
     7981 gcactgatcg ctttccgtca gctggctgaa gataactttg gtgaaacgga ggaagtcgct
     8041 ccacctactc tgcgtcccgt tctgcctgtt tcgggtaaag ccttttatta tcaaccagtt
     8101 ttatgtacgg tacaggcaaa atcaaccctg accgcggaag aagaacaaga tcgattacgc
     8161 caggctattg acttcacgtt attagatctg atgacgttaa cagcgaaagc agaagccagc
     8221 gggcttgacg atattgccgc aatcttttct ggtcaccata cactgttaga tgatccggaa
     8281 ctgctggcgg cggcaagcga actccttcag catgaacatt gcacggcaga atatgcctgg
     8341 cagcaagttc ttaaagaact tagccagcaa taccagcaac tggatgatga atatctacaa
     8401 gctcgctata ttgatgtgga cgatcttctg catcgcaccc tggtccacct gacccaaacg
     8461 aaagaagaac tcccgcagtt taactcgcca actattctac tggcggagaa catttatcct
     8521 tccacagtac tgcaactgga tccggcggtt gtaaaaggta tctgccttag cgccggaagt
     8581 ccggtatccc acagcgccct aatcgcccgt gaactgggga ttggctggat ttgtcagcag
     8641 ggtgagaaac tgtatgcgat acaaccagaa gaaacgctaa cgctggacgt taaaacgcaa
     8701 cgtttcaacc gtcagggtta agccaccgtt cggatggcat cgttctgatg tcatccggca
     8761 atttacccag cactgatgtt ttttaactac ctgttatatt ttttaccaat ttatgatgat
     8821 ttttcttcgc taatcgcgaa ccaaatgtcg ctggctggct aataaacaga cgttatttga
     8881 tcactctatt tcgccgttat ttatcccttt aattcccttt tctaaaatgc ctgaccgttc
     8941 gcagaatgag atttcgatca tgcagctagt gc
//
LOCUS       DADKXM010000146         8903 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.148, whole genome shotgun sequence.
ACCESSION   DADKXM010000146 DADKXM010000000
VERSION     DADKXM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.148"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..2894
                     /locus_tag="JQR05_003391"
     CDS             <1..2894
                     /locus_tag="JQR05_003391"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5325460.1"
                     /translation="VVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRHR
                     NPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTVM
                     MVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESET
                     RFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQLTWPEDLNKDL
                     QQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINELK
                     RTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVWY
                     ECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEVERLL
                     GINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWTQ
                     EEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRNGGSYDVHYSITP
                     LSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTVN
                     STHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEFG
                     LLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMSQ
                     ADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMIAHGVASPR
                     IPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQQAAKAVAS
                     KGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAILDHAESVQKLRL
                     AGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQRL
                     GMKTITGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(3004..3852)
                     /gene="alkA"
                     /locus_tag="JQR05_003392"
     CDS             complement(3004..3852)
                     /gene="alkA"
                     /locus_tag="JQR05_003392"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY5325461.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3986..5338
                     /gene="yegD"
                     /locus_tag="JQR05_003393"
     CDS             3986..5338
                     /gene="yegD"
                     /locus_tag="JQR05_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5325462.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5350..7290)
                     /gene="yegI"
                     /locus_tag="JQR05_003394"
     CDS             complement(5350..7290)
                     /gene="yegI"
                     /locus_tag="JQR05_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY5325463.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALWSFGIETAAGVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(7287..8048)
                     /locus_tag="JQR05_003395"
     CDS             complement(7287..8048)
                     /locus_tag="JQR05_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY5325464.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8045..8704)
                     /locus_tag="JQR05_003396"
     CDS             complement(8045..8704)
                     /locus_tag="JQR05_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY5325465.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRTINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2167 a         2354 c         2328 g         2054 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta
     1081 agctggaatt tcgcattgcc gtgaaagatg gcatccgcca tatccgcgcc ctcgccaacc
     1141 gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg gatatgaccg
     1201 aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac attacgctgg
     1261 attccatcgg tgaagccgtg gtctgtattg atatggcgat gaaaattacc tttatgaatc
     1321 cagtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt ccgctcctga
     1381 cggtgttgca tattactttt ggcgacaacg gaccattaat ggagaacatt tacagtgccg
     1441 acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggaac ggcggcagtt
     1501 acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat ataggctcgg
     1561 tactggtgat tcaggacgtc accgaatcac gcaaaatgct gcgccagctg agctacagcg
     1621 cctcccatga tgcactgacg catctcgcca atcgcgccag ttttgagaag caactgcgta
     1681 tcctgctgca aacggtaaac agtacacatc agcgacatgc cctggtgttt atcgatcttg
     1741 atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggtgacgct ttactgcgcg
     1801 aactggcgtc gttgatgctg agtatgctgc gctccagcga cgtgctggcg cgactcggtg
     1861 gtgatgaatt tggtctactg ttgccagact gtaatatcga aagtgcgcgt tttatcgcta
     1921 cacgcattat cagtgccgtg aatgattatc actttatatg ggaaggccgt gtgcatcggg
     1981 taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg gcagaagtga
     2041 tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc cgggtgacgg
     2101 tttacgaacc acagcaagct gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac
     2161 agtggcggat gattaaagag aatcagttga tgatgatcgc ccacggtgtc gcttcgccgc
     2221 ggatcccgga agcgcgtaat ttgtggctga tttcacttaa gctctggagt tgcgaaggcg
     2281 agattattga tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt agccatgctc
     2341 ttgaccgacg ggtattccac gaatttttcc agcaggccgc aaaagcggtt gccagtaaag
     2401 gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg ctggtgaatg
     2461 atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat ctgattattc
     2521 cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc
     2581 ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata
     2641 tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg
     2701 atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatca
     2761 ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga
     2821 tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt
     2881 tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc gatgctaacg cgtcttatca
     2941 ggcctacaaa tcgctcattc cgcaggcggg ataaggcgct cgcaccgctt ccggcgacca
     3001 acgtcatgct tcgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga
     3061 acgccagggc ttccagcgct cggcataacg gcggatttgt gccggtgtca tccccagaaa
     3121 tcgctgttta atcagataat catccggcag aaaaacatct ttcgcctgcc agccgcgcaa
     3181 ggcgaaataa ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc
     3241 ctgctccaca tcgcccggta ttgtcattgg taaggtgccc tccagcgccg catttgccag
     3301 atgaatcagc gcctctgccc gtttcaacgg catacctaac gcttttaatg cctgcgggtc
     3361 ggctgctgcc agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg
     3421 ttcgccataa agctgtgcca ctctggcggt caattttgcc gccatcgcca cgctcaccag
     3481 ttggcctaaa atcgcccgca cgccctgctc aaaagcatca acactgccgg gtaaacgcaa
     3541 tccgggccgc gccgcgccta acttgcccaa cgcaccgtta acaatctgcg ggttacattg
     3601 cagatcaagc aggcggctca ttttcgccag acactcagcg gcaacgggtt ctaaacctgc
     3661 acttaaattt atgtgcagag tatggcgggc tatatccgga atagcagtca ccacgccgcg
     3721 atattcgccc actgccagac tacgggcata ataactgtcc gcgaccgttt ccacaccact
     3781 caccgcacgg gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt
     3841 caggatatac atcgcatctc cttattcatc cgctttcagc ataaacgtta ttcagacgtt
     3901 acgctttgct ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct
     3961 tcttcaccgg catggggatt atttcgtgtt tattggtttt gattacggta cagcaaactg
     4021 ttcagtggcg gtcatgcgtg acggtaaacc gcatttgcta aaaatggaaa acgacagcac
     4081 gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg gtaagcgaat ggctgtaccg
     4141 ccatcatgac gttccggcag acgacgatga aacgcaggcg ttgttacgac gggcgattcg
     4201 ttataaccgc gaagaagata tcgatgttac agcgaaaagc gtgcagtttg gtctttcctc
     4261 actggcacag tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt
     4321 cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc
     4381 aatgatgttg cacattcgcc agcaggcgca ggcgcagctg ccagaagcga ttactcaggc
     4441 ggtgattggt cgcccgatca acttccaggg gctgggcggc gatgaagcaa acgcccaggc
     4501 gcaggggatt ctggaacgcg cggcgaagcg tgccgggttt aaggatgtgg tattccagta
     4561 cgagccggta gcggctgggc tggattacga agccaccttg caggaagaaa aacgggtgct
     4621 ggtggtggat atcggcggtg gtacgactga ctgttcattg ctgctgatgg ggccgcagtg
     4681 gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac agtggttgtc gtattggcgg
     4741 taacgatctg gatatcgccc tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg
     4801 cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa
     4861 cgacgttcct gcacagagtg atttctacag tagtgccaac ggtcgtctgc ttaacgatct
     4921 ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg
     4981 tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa attgctcttt caagcgtagc
     5041 ggaaacccgc gcctcactgc cgtttatcag caatgaactg gctacgctga ttagccagcg
     5101 agggctggaa agcgccctca gccagccact ggcgcggatt ctggaacagg tgcaactggc
     5161 cctggataac gcccaggaaa aaccggacgt tatctacctg accggcggta gcgcccgttc
     5221 tccgctgatt aaaaaagcgc tggcagaaca gttgccgggc attccgattg caggcggcga
     5281 tgactttggc tccgtcaccg ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa
     5341 gaaaatggct cagcagcggc ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg
     5401 acgtaacggt tccatcaacg gcatggtccg tgctggagca tgaaggcgga atcgctgcaa
     5461 ctctgtcgcg ccgacagtca acgtcgattc cagccgatgt cgtttggcgg ccattttcgc
     5521 cattaccgcc tgtctgtctg ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg
     5581 ttcacagctc gctttccagt cgatgaccgc ctgggtcaga tgatcaccaa accctttaac
     5641 ttgcttaacg ccacgacggg taacacctgc tgctgtttca ataccaaaag accataacgc
     5701 cgctttacgc gcagggccaa cgccgggaat agaggcaaca tcaataaaaa atccctccag
     5761 aaacttctgc ttctgccgtt cccttgcagt gtcatgaagt gctgccagag cgagtttctc
     5821 ttcttccggt aacccgagaa tttcgtcctt cattttttcg agcatcgtcc gtttggcgat
     5881 aaaaccttcc aggccgccta cctgctggat ctggcggact aaatggtcat agtccatttt
     5941 cgcacgatta aatgcctcac ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag
     6001 actgccaata atccagatag ccgccagcac aggaaccaaa ataatataac gcggttctgc
     6061 ctgaaggccg caaagcagca gcgataatgc tgacagtgcg atctcaagca gaatgatgta
     6121 ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa
     6181 tggcagttgc aatgctggcg gtgctactga cgccatcacc atcgcccaga cttttgccag
     6241 cacaaaatcc ccgccggtgg taatgacctc ttcgccgaga tcaataaaat agataacgcc
     6301 ttgattatcc agcgtacacc acgggcagtc ggtcaaatga gcgggataaa catgcattgc
     6361 cgaaacggga catttcttta actgttggcg cagagaatca agtgccgcta cccacgcttt
     6421 agccgtcgga cgcccggttg ccacgccact ttccgtgaat gcctgctgaa acatggcttc
     6481 aacatcactc ggtaacatcg atagcggaat cgatcgtggc ggcggtttta aaccacgtcg
     6541 ctgattatct gacgcgtagg cataacggaa atgggtaata tccgtctcca gcgcattacc
     6601 cgcatcagag ataagcggca caccagaata aggatgccgg ccaccaaaca agacgtgaaa
     6661 aatcagcaac gcaaggccaa aattatcgtg attttctgtg cgttcaaagc cgacaaatga
     6721 cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag
     6781 tgtgccattg gcgttaatct gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg
     6841 acctaccata aaactgttct ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt
     6901 agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg cacaatgagg
     6961 gtaacgttga cgacgatgtg ccgggctata gatcatatga atcggttctt taccagaaac
     7021 ttttggcatc ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg
     7081 ccaggcgaca taattcaaca attgcgcgtc agctgtcgca gccataaagg caagtttgtc
     7141 ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg acgctatcga caaactcatt
     7201 gatatcataa accgcgcctt caccgccttt gcccagttca cggcctggcg taacgcactc
     7261 accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc caacgtttta
     7321 tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa caattcaggt
     7381 aataaatcga gttgttcctg cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa
     7441 ggcacatgcg gagaattatc caacatattt aacgccagtc gctggatacc atccgtaaat
     7501 gctgcaactt tatgcacacg ctcagtgctg gtgaaagttt ccagtctgga aacggcatct
     7561 tcatcggtaa tgaagtgcgt catattggca tattcaccca ccatcggcgt aagcggcaat
     7621 tgcagaccgt gaccaaaatc caccaccacg ccgccatcgc caatctgcat aatcaaggtg
     7681 ccattagccg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct
     7741 ttggcttccg cctcggcaaa aagtcgctga cgaacggtca gcacaatatc cgtggcgagg
     7801 atatcattaa gccccaattc cccaccctgc actttttgcg acatataagc catcgcttca
     7861 ttgacggcga gcatcgcgcc ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg
     7921 aacatcacca gtaatggctg ctggtcattt agccaggcaa cctgcatctg gcaggcatcc
     7981 tggcaaggca agtccgcgct gatgtgcgac gttccaacag cggatgcata aaccagacgc
     8041 cagctcacac tgacgtccag ccttttggcg cttccagtac aacttccgtt cccggcgtgg
     8101 agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg aattgtaatc
     8161 cttgcagagg caaaggctga cgaacgctga tttgtgccag agtcttcata tccgccccct
     8221 gaacaccgat ggaaaagaaa gcaaaccttt tatcttcttc cccccgaaac actttgttgg
     8281 ctgcagcctg ccactcatcg gttggtgcac catcggtaat caggaaaatc cacgggcgat
     8341 aatacgaaat accatttgca cgatactcac gtttccgctc ttctaccata tccagggctt
     8401 tggtaatggc cgcacccatt ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat
     8461 tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca atgccgagtt
     8521 ccactctttt taaagccagc gaatcggcaa gcagctcatc gcgaaaggta accaatccgg
     8581 tgttaagttc attaatagtt ctgccactca tcgagccaga aacatccagc agcagaatgc
     8641 aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc
     8701 tcataagaaa tcctttctta gagaattatt gaggtccggt atcataccga ctgcaataat
     8761 gaaataagag caaacgaaga aactttcaga attagtcccg gcggcgcaga aaattttgct
     8821 attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg acataatttc
     8881 aggagtgagg gttagggaga ggt
//
LOCUS       DADKXM010000147         8830 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.149, whole genome shotgun sequence.
ACCESSION   DADKXM010000147 DADKXM010000000
VERSION     DADKXM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.149"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(232..990)
                     /locus_tag="JQR05_003397"
     CDS             complement(232..990)
                     /locus_tag="JQR05_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY5325466.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(998..2101)
                     /locus_tag="JQR05_003398"
     CDS             complement(998..2101)
                     /locus_tag="JQR05_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY5325467.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2111..3292)
                     /locus_tag="JQR05_003399"
     CDS             complement(2111..3292)
                     /locus_tag="JQR05_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY5325468.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3360..4385)
                     /locus_tag="JQR05_003400"
     CDS             complement(3360..4385)
                     /locus_tag="JQR05_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5325469.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4816..5037)
                     /locus_tag="JQR05_003401"
     CDS             complement(4816..5037)
                     /locus_tag="JQR05_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325470.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5290..8386)
                     /gene="acrF"
                     /locus_tag="JQR05_003402"
                     /pseudo
     CDS             complement(5290..8386)
                     /gene="acrF"
                     /locus_tag="JQR05_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8398..>8830)
                     /gene="acrE"
                     /locus_tag="JQR05_003403"
     CDS             complement(8398..>8830)
                     /gene="acrE"
                     /locus_tag="JQR05_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAY5325471.1"
                     /translation="VELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTL
                     LPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEARPVVASQAIGD
                     KWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2382 a         2306 c         2094 g         2048 t
ORIGIN      
        1 catcgaataa aacaaaaggc ccagtcttcc gactgagcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 cgaaaggcca tccgaaagga tggccttttt gcttttcgaa ctaacattca attaatggat
      241 tacctgcgat aaaaatgccc tcgtacgctc tgatttagga tgcgcaaaaa attcatcagg
      301 tgtagcttgt tccactatct ccccacgatc cataaaaatt acccggtcag cgacggtacg
      361 tgcaaacccc atctcatgtg ttacacacaa cattgtcata cccgactgcg ccagcccaat
      421 cattgtatcc agcacctctt tcaccatctc cggatcgagc gccgacgttg gttcatcaaa
      481 caacataatt ttcggcttca tacacagcga acgcgcaatg gcaacgcgtt gctgctgacc
      541 acctgaaatc tgtccgggaa acttatgcgc atgttcggca attctcaccc gctcaaggta
      601 atgcatcgcc agagcttcag cctctttctt aggcatcttg cgtacccaaa tcggtgccag
      661 ggtacagttc tgtaaaacgg tcagatgagg gaagagattg aaatgctgaa ataccattcc
      721 cacttcctgc ctgacgcgct caatattgcg gatatcttca ttaagttcga tgccatctac
      781 gacaatccgt ccctgttgat gttcttccag atgattaata caacgaatgg ttgtcgattt
      841 accggaacca gaagggccac acagaacgat ccgttctccc ggttgcacgg ttaaatttat
      901 atttttcaaa acatggaatt gtccatacca tttatggacg ttttccagcg taatcatcgc
      961 gttagcaggt tgcagtaaaa tttggctcat agtgtcctca atgcggtgta cgcccggtgt
     1021 taaaacgttt ttccagatgt tggctataac gtgacatgct gaaacaaaag atccagtaga
     1081 tcagcgcggc gaaaacatac ccttccgtcg acatacccaa ccaggcggga tcaacggttg
     1141 cctgctgaac gctactgaaa agatcgaaca agccgatgat gatcaccagg ctggtatctt
     1201 tgaagagtgc gatgatggta tttaccagcc caggaattac cagcttcaac gcctgtggca
     1261 gaataaccag cccctgagtt ttccagtaac ccaacgccag cgactctgcc gcttcatatt
     1321 gccctttagg cagcgcctgt aatccacctc gcacgacttc cgcaacatat gctgactgaa
     1381 acaggatcac gccaaccagc gcgcggatca gtttgtcgat actggtgcct tctgccataa
     1441 acaacggcag catgaccgaa gacataaaca atacggtaat cagcggtacg ccgcgccaga
     1501 actcgataaa aatgaccgat aagatacgca caatcggcat atgggagcgg cgacctaatg
     1561 ccagtaagat cccccacggt agcgcccccg caatcccaac ggatgcaata attaaagtta
     1621 gcgtcagccc gccccattgc cgggtttcaa cccgctcaag accaaaaaaa ccgccataca
     1681 tcagccacca gacaatcagt gggtaaatca ccgcccaggc cgcaatatag cgaccgcgat
     1741 gcgggagtat tttccagaac attggtgcga tcgatacaag cccaatcagt aatgcgaggt
     1801 taattcgcca gcgttggtcg tgtgggtaaa gcccatacat aaactgacca aatcgttcgt
     1861 gaatgaagac ccaacaggca ccggcttttg tgcagtcagc acgcgttgag ccaacccaat
     1921 tagcctgcaa aaatgcccag ttcagcaacg gtgggatcag ttcccacatc aaccatatgc
     1981 agccaatagt cagcaggcta ttgctccagc tggagaacag attttttcgc acccacacca
     2041 tcgcgcggct tgagttatgg ctcgccgggc gcgagggctg agacagcaat acttttgtca
     2101 tcatgactcc ttagcgttca acgatcgcga tgcggcggtt atagatattc atcagcagcg
     2161 agatagtcag gctgataatc agatagaccg acatggtcat ggctatcgtc tcgatggctt
     2221 gccccgtctg gttcagcacg gtgccagcaa acagcgaaac catatcggga tagccaatag
     2281 cggcggcaag agaggagttt ttgacgatgt tgagatactg gctggttaac ggcggaataa
     2341 ttactcgcag tgcctgggga ataatgacct ggcgtagcgt aaccgggttg ggtaacccca
     2401 gtgaccgagc cgcttcatgt tgaccataag gcactgcctg gatcccagcg cggataatct
     2461 cggcgataaa tgcagaggta taaaccgaaa gtgccagcgt taaggctgcc agttcaggaa
     2521 ttaaaaccat tccgccgcgg aagttaaagc cttgtagggc tgggacatcc cagtgtagtg
     2581 ctgcgccaaa aagccattgc gccagtaaag gcaaaccaat gatcaacact gcggcgatcg
     2641 gccaggagcg gcgcagttgt ccggtcttta tctggtatgt tttattaaag cggaatagcc
     2701 caacagaaag gactatagcc ataacaacag ccaggataaa cgcaataaat ccgtctccca
     2761 gctgcggtga cggaatataa agcccacgat tgctcaaaaa ggccagatca aacgcgctca
     2821 cagcttggcg tggtccgggc aaattgcgca acacggcaaa gtaccagaag aagatttgca
     2881 gcagcggggg aatattacgg aagatctcaa tataaattgt ggaaagcttt cgtagcagcc
     2941 agttatccga aagtctcgcc agaccgataa agaagcccaa aacagaagcg aacacaatac
     3001 acaatgcaga aaccagtagc gtattgagta agccgacaat aaaaacgcgt ccgtaggtgt
     3061 cgccctgctg gtaatcgatc aaatgctgga caataccgaa gccagcgccg cgatccagaa
     3121 aggcaaaacc tgaagtaatg ccacgattat tgagattcgt tacagtgttg tgaaataacc
     3181 aaccaacaat gccgacaaca gcaacaacgg caaggatctg gaataaccag gcacgaaccg
     3241 tagggttggc aaaggagagt gagcctttaa cggttgagcg gcgatgagac atagaaacct
     3301 cggaaagcat aacgctgaag agagcgccgc cgtagcgacg ctcgttacct cacaccgact
     3361 taacgcacgg gcggcgcgta ctgaataccg ccgttattcc agagattatt ttgcccacgt
     3421 ttaattttca gcgggctttc tgaacctacg ttacgctcaa aaatttccga gtagttaccc
     3481 acctgtttga tgatgttata ggcccattta ttatccaact tcagatcctt gccgtaatcg
     3541 ccttctttac ccaacagatg tgccatatca ggcgttgctg gattagccgc tttttcatcg
     3601 acgttctggg aattgatgcc catctcttca gcatttagca tggcgaaaag cgtccagcgt
     3661 acaatcgaga accattcatc atcgccacga cgaactaccg gcccaagcgg ttctttagag
     3721 ataacctccg gtaagacaat ccattcagcc gggttgctta atttgatgcg caaggcatac
     3781 agttgcgact gatccgaagc cagcgtatcg cagcgaccag attccagtgc cttcgctgat
     3841 tcgtcagagc gatcgaaagt cactggtgtg tacttcatat tgtttgcctt gaaatagtcg
     3901 gcgacgttga gttcggtatc agtacccgcc tgaatacaga cggtagcgcc atccagttct
     3961 ttcgcgcttt ttagccccgc tttatcgtgc gtcaggaagc caatgccgtc gtaataagtg
     4021 acgccggtaa atgccattcc catcccggca tcgcgagatg aagtccaggt cgtattacgg
     4081 gagagcaaat ccacttcccc tgactgtaaa gcggtgaagc gttcttttgc tgtgagtggg
     4141 gtatatttca ctttcgtgtc gtcaccaaat acagcagcgg caacaccacg acaaacatca
     4201 acatcaatac ctgaaaactt accgtctgca tcggcataag agaacctagg taatccatca
     4261 ctgatcccgc attgcacaaa accttttttc tgaacggcat caagcgtcgc gccagcatgc
     4321 gcctgatttg caacggcaag cagcacgctg gcggcagcca gtgtggctat catcatcttt
     4381 ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg acgtttggga aacgtctttt
     4441 acctgtctgt ggccatcctc ttataagcaa aagtagtgcc agcttttgcg cacgatttcg
     4501 cgtcgcaata agctgagtgt agacaggaaa taataaaaac agcgccccgc aatggtgcga
     4561 aaatgaaaca tgcgccacat tttggcgcaa aatatttacg cgtgttttac taagcagaat
     4621 aatgattgtt agcgtgagta tatttctggt gggaagtgtg aaagttaaat tgatgcgtta
     4681 tttcgaaagc agcgaaataa cgcgatataa catgctaaat cagcagagaa ttagccagca
     4741 gaatatagca atattttatc ttatgtaaag atactgtatc gttaaacaga gcgcgaaact
     4801 ccgcgccctg ttcagttatt tcgacagagc gatgatcccc atcgtcaggc cagcaacagc
     4861 cgctgccccg ccagcaatag ccccagccgt ttcccagtta tcctgagtcg tgcctttcat
     4921 acaggtattg gtgtgaacca gacgaccctg atcgtcataa accggaacgc agggcgaatc
     4981 gtgagcacag cccgccagca atgcggtgag caatgccaca ggaattaatc ttttcatggt
     5041 gttacctcag taataaacgc taacgcttaa taagttaact ctcttattaa cgagctgaat
     5101 gccattttat caccggaggt tataaatctt gcggaatgaa taatgacaaa ttatgtgaat
     5161 tgtaaaaata gtgaagggag cgggaactat agaaagcttt taaagagtga gcagaagaat
     5221 atcttattaa tttttatggg tttcactgga aataataaag gcacccgaaa gcgcctttat
     5281 gtttctgatt tatcctttaa agcaacggcg aatcaccaca aagaacaccg gtacgaagaa
     5341 gattgccagc aacgttgcag agaccattcc tcccattacc ccgataccca cagcgttctg
     5401 cgcgccactg ccggcaccgt tactgatagc tagcggtaat acgccgagaa taaaggcgag
     5461 agaggtcatc aggataggac gcagacgcat acgtactgcc atcagtgtcg cttcaacaac
     5521 acctttaccc tctttctcca tgagatcttt agcgaactca acaatcaaaa tagcgttttt
     5581 ggccgacaag ccaattgtcg ttagcaagcc caccataaag tagacgtcat ttttttgatt
     5641 aaagagtgtc gccgccagca gcacgccgac aatccctaac ggcacgacca acataaccga
     5701 gacaggaatt gaccagcttt catagagtgc agcaaggcac aggaaaacaa ccacaaagga
     5761 aattgctacc agagcgggag cctggtttcc cgataagcgt tcctgatacg acatacccgt
     5821 ccagtcataa ccaatgcccg caggtaattt tgacgcaagg ttttccatca acgccatggc
     5881 atcgccggaa ctggttcctg gcgcggcttc cccctgaatc tccattgacg gcagaccgtt
     5941 gtagcgttcc agtcgcggag agccatacac ccaatgtgaa gtggtaaagg cagagaatgg
     6001 caccatttcg ccgttggcgc tgcggacata aagtttatcg acatcttctg gcagcatacg
     6061 gaatttggca tccgcctgaa catacacctt tttcacgcga ccacggtcga tgaagtcgtt
     6121 aacgtaagtc ccacccaacg ccgttgaaat ggtctgattg atgtcagaaa gtgaaacacc
     6181 taatgccgcg ccttttcctg gtcaacttcc agtttaaact gcgcggtgtc ttccaggcca
     6241 ttaggacgca cgctgactaa gctggcagga tgttgcgccg ccataccaag caactggtta
     6301 cgggcctggg ttagggcatc gtgacccagc ccagcctgat caattaactc aaagtcgaaa
     6361 cccgttgccg tgcccagttc aacaatggct ggcatattga atggaatgac aaaaccgtcg
     6421 cggatcttgc ccaattccat tttggcacga tggattaccg cttccgcact gttttcatca
     6481 ccattacgct cttcccacgg tttcagactg acgaaggcca taccggcgtt ttgtgcctgg
     6541 ccgctgaagc taaagccgtt aaccgtaaag acactttcaa cgttcgcttt ctcgttcttc
     6601 agatagtaat ccgtcacttg atccaacact ttttgcgtcc gctcttgcgt cgcgccagcg
     6661 ggtaactgaa tcatggtcag aaagacaccc tgatcctctt caggtaagaa ggaagacgta
     6721 aaaacaacac caccattcct gcaacaatca gcgcatagat cagtaaatat cgtcctgtgg
     6781 aaccgaggat tttgccgacg ctgttggtgt agtggttaac gctatgatcg aaggtggtat
     6841 taaaccagcc gaagaaaccg cccttatttt cgtgatgctc agcagagacg ggtttaagca
     6901 gcgttgcaca taacgcaggg gtaagaatca atgccaccag aacagaaagt gccattgccg
     6961 aaacgatggt gatagagaac tggcgataaa ttgccccagt agaaccgccg aagaatgcca
     7021 tcggaataaa taccgctgac agcaccatcg cgatacccac cagtgcgccc tgaatttgcg
     7081 acatcgattt ttccgtcgct tcttttggcg ggagcttatc ctccatcatc acgcgctcga
     7141 cgttctccac caccactatc gcatcatcga cgagcagccc gatggcaagc accatcccga
     7201 acatcgttag tgtgttgatg gagtaaccaa aagcggcgag gatggcaaac gtccctaaca
     7261 acaccacggg taccgcaatg gtggggatca gcgttgctcg catattctgc aagaacagat
     7321 acatcaccag gaacaccagc ataatggctt cgaacagcgt ttttaccact tcgtgaatag
     7381 aaagctggac gaatggcgtg gtgtcataag ggtagagaac cttcattccc tgcgggaaga
     7441 atggctgtaa ttccgccagt tttgccttaa tggctttcgc ggtatcgaga gcattcgcgc
     7501 cggttgccag cttaatcccc aggcccgccg ccggttttcc gttgatacga gcgataacgt
     7561 tatagttttc accgccaagt tcaacccgtg cgacatcttt caggcgtacc accgagccgt
     7621 cactgtttac gcgcagggtc actttgccga attcttccgg attttttaac cgcgtttgag
     7681 caataatcga ggcattcaac tgttgccctg gtaacgctgg cgttccgccc aactgtccgg
     7741 cagcgatctg atcgttctgt accttcaact ggttaatcac atcaaccggt gtcagtttat
     7801 atttgtttag cagatcggcg tccagccaga tacgcatcgc atactgtgcg ccgaaaagct
     7861 gtacgtcacc gacgccattc agacggctaa gcgtatcttt aacgttagag gccacatagt
     7921 ccgagatatc gtcctgtgtg gtgtctgggt tatcagagac aaagcccgcc accatcaaat
     7981 agctgctact ggacttttca acactgatcc cctgctgctg aacctcctgc ggcagcaacg
     8041 gcgtggcgag ctggagtttg ttctgcacct gcacttgcgc gatatcagga tcggtcccgg
     8101 actggaaggt aagggtaatt gtcacgctac cggcggaatc gctggtggag gacatataca
     8161 tcaggttatc gataccattc atattctgtt cgataacctg cgtcaccgta tcctgcacgg
     8221 tctgcgcatc agcacctgga tagtttgccg aaacagaaac cgcaggcggt gcaatggttg
     8281 gatacagagc gacgggcaat tgtaggatcg ccagtgcgcc cgccatcatc agaataatgg
     8341 ccagcaccca tgcaaatatc ggtcgtcgaa taaaaaagtt tgccatgtca gattacctta
     8401 cttcgatgca gtatctgcgg gggtatcggt agtggcttta acctgctctc ccggacgcgc
     8461 tttttgcagg ccgctgacaa tgacttgatc gccagatttc agaccttcgc taatcaacca
     8521 tttatcgcca atcgcctgac tggcaacgac agggcgcgct tccacctgac ttttatcgtt
     8581 aacaatcagc acggttgcat caccacgcgg tgtgcggcta acgccttgtt gcgggataag
     8641 aatggcgtca ggttggacgc cttcatcaat ccgtgcacgc acaaacatac ccggcaaaag
     8701 cgtatgttgc gggttaggga agacagcacg tagggttatg gagccggtgc tttcatcaac
     8761 ggtcacatca gagaattgca gcgtaccttt caggggatag gtttgaccgt tttccatgac
     8821 caactctacg
//
LOCUS       DADKXM010000148         8772 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.150, whole genome shotgun sequence.
ACCESSION   DADKXM010000148 DADKXM010000000
VERSION     DADKXM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.150"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..948)
                     /locus_tag="JQR05_003404"
     CDS             complement(<1..948)
                     /locus_tag="JQR05_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY5325472.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
     gene            complement(1035..1396)
                     /locus_tag="JQR05_003405"
                     /pseudo
     CDS             complement(1035..1396)
                     /locus_tag="JQR05_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1736..2290)
                     /locus_tag="JQR05_003406"
                     /pseudo
     CDS             complement(1736..2290)
                     /locus_tag="JQR05_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2433..2555
                     /locus_tag="JQR05_003407"
                     /pseudo
     CDS             2433..2555
                     /locus_tag="JQR05_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2817..3383)
                     /locus_tag="JQR05_003408"
     CDS             complement(2817..3383)
                     /locus_tag="JQR05_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325473.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYCISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3665..3979)
                     /locus_tag="JQR05_003409"
     CDS             complement(3665..3979)
                     /locus_tag="JQR05_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325474.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(4057..4167)
                     /locus_tag="JQR05_003410"
                     /pseudo
     CDS             complement(4057..4167)
                     /locus_tag="JQR05_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4491..5168)
                     /gene="ospD1"
                     /locus_tag="JQR05_003411"
     CDS             complement(4491..5168)
                     /gene="ospD1"
                     /locus_tag="JQR05_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY5325475.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMISNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5539..5676)
                     /locus_tag="JQR05_003412"
                     /pseudo
     CDS             complement(5539..5676)
                     /locus_tag="JQR05_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5755..5961)
                     /locus_tag="JQR05_003413"
     CDS             complement(5755..5961)
                     /locus_tag="JQR05_003413"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325476.1"
                     /translation="MDAENETVLNANMTHHLGCEKNQLRSGSNSRNGCLTKIITTGDE
                     PLEIRTLRDRNGTFEPQQLKKNQP"
     gene            complement(6645..6804)
                     /locus_tag="JQR05_003414"
                     /pseudo
     CDS             complement(6645..6804)
                     /locus_tag="JQR05_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6808..8037)
                     /locus_tag="JQR05_003415"
                     /pseudo
     CDS             complement(6808..8037)
                     /locus_tag="JQR05_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8057..8404)
                     /gene="tnpB"
                     /locus_tag="JQR05_003416"
     CDS             complement(8057..8404)
                     /gene="tnpB"
                     /locus_tag="JQR05_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5325477.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(8404..>8772)
                     /locus_tag="JQR05_003417"
     CDS             complement(8404..>8772)
                     /locus_tag="JQR05_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325478.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2223 a         2072 c         1968 g         2509 t
ORIGIN      
        1 attttcacca ggatgcacgt catactcttt tttctccgga ggcagtgcca gcatggactg
       61 ctgctcttcg agccagcgat cacagggacg ggcctggatt gtttcatgct ttcgttggtt
      121 agcgacatcg tgcagccagc gcagaccgtg gcggttggct gtttcaacat cgacagtgct
      181 ccccatcggg cgcaggcgag tcattagtgg gatgtaaaaa ctgttacggg tgtactgcac
      241 catccgttcc accttacctt tagtctgtgc cctgaagggg cgacacagtc ggggagagaa
      301 gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac cggtctgata
      361 tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc gcggcacacc
      421 accaaagaag cggaacgcat tacgatggca ggtctccagc gtgtcataac gcatattgtc
      481 agtgaattcg atgtacaaca ttcggctgta tccgggaaca gcaacgaaca cgtgaagcgg
      541 tgagcgacca ttacgcatag tgccccagtc aacctgcatc tgtcgtccgg gttcagtttc
      601 gaaccgaacg gcaggctcct gctcctgagg aaccgagaga gaacgaatga atgccctgag
      661 aatggtcatt ccgccacgat atccctggtc tctgatctcg cgagcgatta ccgttgccgg
      721 gattttgtaa ggatgagcat cggcgatgcg ttgacgaata taatcccggt attcatccag
      781 gagtgaagca acagcaggtc gcggcgtata ttttggcggc tcagattttg cctgcaaata
      841 acgtttaacg gtattgcggg agagccccag ttctctggca atcgcccggc tactcattcc
      901 ctgcttgtgc aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga
      961 atcaggagag cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg
     1021 caccgttcgt gacagtgttg ttgcgccttg ctgccaacga agctccactg cccatcaagt
     1081 tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg ccggggcggg
     1141 agctttttaa tgtccgtatg acggtattga tgccgatttt cagcgtacgg gcagtatcgc
     1201 gaactccggc accgttgaaa gccatttcag tgatttggtt tttaactccc ggtttacggg
     1261 cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc acggcaacgg aatcgatcat
     1321 gtccttttgg gttctgcccg tgacggtaaa cctgaacgga ctggcaacgg gggcaatgaa
     1381 agttaactgt gaccatgaaa gaaccttaaa agcgggtatt ataaccccta attcaactaa
     1441 ttggaggcat tgtcattttt aatctatgga tacacttaaa aatcctctca ccttacgtta
     1501 aaaaccttct catgtttaga gttttttatt atgtttatca taatgatatg acgatctgag
     1561 tttttaaaat caagaagaaa agataagttt tttaaatgca cagtgcctgt ggataacttt
     1621 gcgtaaatcc ggaggacaga tcactctgaa gcaagacaac agcatctgaa aacaatatgc
     1681 tcacgtttca accacagggc gtaagtgatc ctacccacgt aatatggaca caggcctaag
     1741 cgaggttctg gttttcaaat tgttccggac tgaggccgcc acaccaactg tgccgtcgcc
     1801 agcgattgta atcacattcg atataattaa acactgttgc ccgcattatt tcccggctga
     1861 taaagtgttc tccgtggaga cattccactt tcagcgaatg aaagaagctt tccacgcagg
     1921 cattatcgta gcagcagccc tttgcgctca tactgccacg cagattatgc cacttcagca
     1981 gcgcctgata atctgctgaa cagtactgac ttcctcggtc cgagtgaacg ataacgttcc
     2041 ggggtctctt acgccgccac agcgccatct gcagggcatc gcaggccagt tgcgccgtca
     2101 ggcgtggtga catggaccag ccaataacgg cacgtgacca caggtcaatg accacagcca
     2161 gatacagcta gccttcatct gtacgtaagt acgtgatgtc tcccgcccac ttctggttcg
     2221 gaccactggc gtaaaaatcc tgctccagca gattttctga cacaggcagg ccatgtgcgc
     2281 ggtaactgac cgtattgctg ggtaagatca atttgctcac tggtatttac tacgtgcatc
     2341 gaatgagtga tcaagagccg tatgacgcga gagtgtcacg tacggttccg tgagcagccg
     2401 gagagtgaaa ctctctccgg ctgactcgac tcgcaggttc cgacagcggt gacgaacatg
     2461 cagcggtgtt gttctcgctg atcggcacat gccgtctgaa caatgtggag ccagaaaaat
     2521 ggctgcgtta cgttattgaa catatccagg actgaccggc aaaccgggta cgcgatctgt
     2581 tgccttggaa agttgatctg acttctcagt aaatatcaat atgattctga cgagccgctt
     2641 acctttgagc agagaaaggg gagagtgatc ccacatccat gagctaggaa aatacaaaaa
     2701 ctggctatac aacaattcaa tatattagga cttacttgcg aggagaaata tagcaccgct
     2761 caggcaattc ctgggtattt acttttctta gtttctttac tcgggatgtt ctgtcatcag
     2821 aaaggcgatt ctaaatttgt aatatagtca ctcatattcc cacgtaattt ctttagatca
     2881 atatattgca catttttctc aaaaaactga tcggaaataa gatttgcaac attgcttgtt
     2941 gtatcatttt taacgcttct attcatccct tcacaaagtt cgtccatttt ctttttgaaa
     3001 taactgcttc ctgatacaag atgatttatg gaactttgtg cagcattgat gtctagagaa
     3061 atgcaataat ctttctcaac ctgtttaaat atctgtttac ggagattatc gccgattttt
     3121 cggctgtgtt ctgcacaaaa cttatcaata cattggttta ccacccggga tacgtagact
     3181 tttttttcat catttttcca atctgtaata ttttcaccga tttgttctga aacagagctc
     3241 aaaatactag tatttatttt ggttgatgat atttttacta tccttttccc cattgtacag
     3301 cggataatct catccacctt tcctgaaaaa tatcttttgt gagataaact gattccaaaa
     3361 ttattaaaag atgttccaag cattataatc actttgtcat gtaaaatagt aacattgtca
     3421 ttattttagc tgattcaaac aattctgtaa atgctttgag ttctttactc ccttggctca
     3481 atgatggacc gtctggaatc ccagagacta aactctctga gaaagagagt atgaaaaaac
     3541 ttggggcagt ccctaaatga agggaccaac agcggtaaaa taaccggcat cattcataat
     3601 gatgccctgt tagttgtagt tatgaagatt tacgtcatta aaattgctgt tcgtagtgtc
     3661 agtcctatgg tctggcatca gctgaggatc gtagccgata catcactggc ttcacgacat
     3721 tcgtgttgag gctattcatg aaaactccac gctgaaaacg ccgttttgta taaacggtca
     3781 cggtatgccc ggagccactg ttgctgatga attcgataaa accttggcgt ttcttgaagc
     3841 tatcgttaat gcggataatg aaacaacgat tggtgagatt cgctcttttg ccgacgccct
     3901 ggacgctgtc cggttcaatc gcaacaaaat caaccggcag ctgagcaaac ccaaccttgc
     3961 ctctctggcg ctggagcatg aagtgatttg gctgggacgt agccgctgac agcttctata
     4021 aaagtatgat cctacccacg taatatgaac acaggcttaa gtgaggttct ggttttcaaa
     4081 ttgttcagga ctgagacctc cacaccaact gtgccgtcgt cagcgattgt aatcgcactt
     4141 gatataatta aacactgtat atttcatttg tggagtctcc agtctgaggg ggctttatgc
     4201 attccgtatt gggcactatg atgccagaaa tctgcaaggc tccacacctt cctgtatttc
     4261 ccttcctcta tcacagtgca ctttacttaa tagggtggga tgtgttcatt acatttattg
     4321 tgttttttat gattgttttt gcgtccagtg tcgttcgtca tgggaaactg gttctatggg
     4381 tagtatcgct cagtctagtt aactgtttgt ttctttttag aaattaagca gatcacacaa
     4441 ttcggggaga gatccttcta agtgcaattc tttgcagcta tttagcacac ttaaaacaac
     4501 tcttgtattt tgtcagaaat aatattaaac atgtcttgtt cattattgtt aagtctgtct
     4561 gctatttctg taagttttat cccactatga tggcatatat ttatatatgc catcatagcc
     4621 tcgatattcc ctttccctgc ggccagaaat aatcctggta acccctcatt atctcttaca
     4681 tccaatagtt taatcgtttc ttttggtgat aaatcaaaag ttttaataaa atctccataa
     4741 gcttgaatcg cagcggaatg accttcttga agagctatga acaaagcaga ttccccagca
     4801 ttatttcgtg ctgcaagaac ttctagtttt tcctctttgg gtatttcggc tgccaacagc
     4861 gattcaccgt atttacgcgc tgcatcggca tttccatgcc aaatcaatga aaacaaatta
     4921 tggcggttat actccttatt attctttgtc ggaatggata tgctctgtat tttggccaat
     4981 tttctgaaag aaacaccact atatcccccc cctgattttt cttcattgat ggcgtgggat
     5041 atagcggtat ttgtcacgtt aatgatatta cttatcattc ctttattttc attagcagta
     5101 ttgctgccta ttattaggtg catatttttg ttgtttgctg gatgtaatcc atagttattt
     5161 attgacattt ttgttcatcc tttttgctaa aaaaaacaat aacctatttt tgtaatgtaa
     5221 tagatattag tctattgcta atctaaaaaa accattgcca cattgaatgg ctatattcac
     5281 caataaattc tttcgatttc gtatattgac atatcgtaga tatatcttat ccctaagatt
     5341 caataatcga gtccgtttac cattctgtgc aagcgccttc actcagaagt gaccgtcaag
     5401 gcgatcacca aacttgataa taaagcagct cattgccata cgccagtcct taaggggcgt
     5461 tgtccatttc tgtgatgctg cctagattta actgccagct ccggcttatc tacatctaaa
     5521 tatgtgagcc taataccgct acactttggc cagactatgt ttttctccgg gaagtgggct
     5581 aaccgtctcc ataaagtgac gaactttttt caacagtggc catattgaac ggcactgatg
     5641 attacgggtt attgtctcat gaagatccgg ccacatcact gacagcagtg acttcaggcc
     5701 ttcagcacca gacagcctga accagttaat ccatcgcccg acagatttag taatctaggg
     5761 ctggtttttc ttcagctgtt gcggttcgaa agtgccatta cgatcacgca gagtacggat
     5821 ttccagaggt tcatctccag tagtgataat cttcgttaag caaccattgc gggaattgct
     5881 gccagatcta agctgatttt tctcgcaccc aagatgatga gtcatattag cattgagtac
     5941 ggtttcattt tcagcatcca atcaaactga ctgagatctt caggattttt gagacttttg
     6001 gctagttcat tagctaaagc ccgcaactta ttagatgtca actgtttttc attcataaat
     6061 tcaccttcat ttgatatcaa attaaatagt tcaacatcag agaattatac aaacttatgt
     6121 acaggctcct atgatcttcg aatcctgaag attatagtga aaataagttt tcacgttgaa
     6181 atcccacata gttagtatat gataaataat catggatgac ctgattaacc tcttggggta
     6241 gtgcttttat agaagaataa atattctact aatttaatac atcacctaaa atagtattgt
     6301 tttttatggt tggttgaagg tcgtggtgat gatgttagtc cttttgagaa tattccagtg
     6361 ccagatctta cttataataa aggggcttgg cagtttggat aaactattgt tcttattcca
     6421 ctaatacaaa ctttttgtaa agtatattaa atatacttca tttcatactg aaatccccgg
     6481 aagtattcaa tcaaacaatt acaccataaa aggaagaggg ggccctaatt aagttgtcaa
     6541 gcatgtttat gatacctgca gggtataaat tttctggctg caagcagaca catataaaac
     6601 gtctttattg atcctaccca cataatatgg acacagtcgt aagtgaggct ctggttttca
     6661 aattgttccg gactgagacc accacaccaa cggtgccgtc gccagcgatt gtaatcgcac
     6721 tcgatataac taaacactgt tgcccgcact atttcccgat ggataatagt gttctccgtg
     6781 gagacattcc attttcagcg aatgaatttt catctgttgc tgtatccagt catacagtga
     6841 ctgcatcagt ggcgcggctc tggcttttct tgccgccaga cgctgttctg ctgaacatcc
     6901 ccggacttct gcctcgatgg catacagttc accgatacgc tgcagggctt ccgtggtgat
     6961 gtcggttggc actcttgcat gcacatcgtg gatttttctc cggacatgag ccatacatgc
     7021 ggcttccgtt attctgccgg attcgtacaa cgcccggtaa ccaccgtaaa catcggcctg
     7081 aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg atacctttcc ggtccggtga
     7141 gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg ttacggtcat cacgaacgta
     7201 gacccacagc cgggcagtcc ttgttttacc gctgcccggc tcctggaccg gaacggggat
     7261 atcatcggca tggactttac cgggcatcag cacatactgg cgcaggatgt catacagcgg
     7321 ttccagcagt tcagcaacag cacctgtcca gcgccccagt gtggcacggc tcagctccac
     7381 gccctgacga cggtatattt ctgactggcg gtataacggc agatagtctg catattttcc
     7441 agcgacaaca tgggccagaa gccccgctcc ggcataactg cgtgcaatgg gttttgaagg
     7501 caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc agtttcggac gttgtgtttc
     7561 gataacctta aaggcgctgc tgataagctc cagctgctct gacacatcac atcccagaga
     7621 actgagttcg ccaccacagg ccgggcagca ttcctcttcc ggacggatga cccgggtttc
     7681 acggggaagt gaggcaggta acggtttacg ggctgaagac tggcgcaggg gagatggcag
     7741 taccgggtca tattgctcac ccagcgtttc cgccatttct tcctgaagtg cgctgattcg
     7801 ctcctgtgct tccagtatct gccgttcggt ttttgcgcga agtttttctg agcttttacc
     7861 gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc cggttgattt cggaagcata
     7921 agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc atctggcgga tggtgtcctg
     7981 ttgcgtctgc aacagtgccc gcaggcgggc attctcatga gcaagtgagg tgtccatatc
     8041 ctcactttac aacgggttat atgcggatgc cagcgcgttc cgttcgtttc gggtgtttcc
     8101 agtcgatgcc ctcaagaagc atggataact gggccggagt aaggtgcacc ttgccgtcac
     8161 gggtgaccgg ccagacgaag cggccccgct ccaggcgtct ggtaaagagg cacagtccgt
     8221 cactgtcagc ccacaacact tttatctggt caccccggcg tccgcggaag atgaacagat
     8281 gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt tgaggccagg ccgttaaagc
     8341 catttcgcat atcggtgata cctgcaacca gccagatacg cgaacctgca gggaaagata
     8401 tcatcagtgg ctgctccctt ttatttcgcg gataagtatc tgtaataacg ccggcgtcag
     8461 tttaccttta agcctgagag ttccggccgg cagaaccagc tcacagcaca gactgtcgga
     8521 cggtgtattt atctgctctg gttcctgtgc cggagccggg attttattat ccggctccgg
     8581 cgttaacgtc acgggaagca gtgcaggcat attttttccg gaaggcagca ggccaccttt
     8641 ccggtattga tggcgccagt tgaagagcag gttatcgttg attccgtttt cacgggcgat
     8701 ctgcgccaca caggctccgg gctgcagtga ctgctccact aaggcgattt taaattcata
     8761 aggaaaatta gg
//
LOCUS       DADKXM010000149         8770 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.151, whole genome shotgun sequence.
ACCESSION   DADKXM010000149 DADKXM010000000
VERSION     DADKXM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.151"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(524..892)
                     /locus_tag="JQR05_003418"
                     /pseudo
     CDS             complement(524..892)
                     /locus_tag="JQR05_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1416..2390
                     /gene="hemB"
                     /locus_tag="JQR05_003419"
     CDS             1416..2390
                     /gene="hemB"
                     /locus_tag="JQR05_003419"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY5325479.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLNLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2496..3347)
                     /gene="tauD"
                     /locus_tag="JQR05_003420"
     CDS             complement(2496..3347)
                     /gene="tauD"
                     /locus_tag="JQR05_003420"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY5325480.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3344..4171)
                     /gene="tauC"
                     /locus_tag="JQR05_003421"
     CDS             complement(3344..4171)
                     /gene="tauC"
                     /locus_tag="JQR05_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY5325481.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4168..4935)
                     /gene="tauB"
                     /locus_tag="JQR05_003422"
     CDS             complement(4168..4935)
                     /gene="tauB"
                     /locus_tag="JQR05_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY5325482.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4948..5874)
                     /gene="tauA"
                     /locus_tag="JQR05_003423"
     CDS             complement(4948..5874)
                     /gene="tauA"
                     /locus_tag="JQR05_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5325483.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(6142..6657)
                     /locus_tag="JQR05_003424"
     CDS             complement(6142..6657)
                     /locus_tag="JQR05_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325484.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARAWGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGCKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6850..7101
                     /locus_tag="JQR05_003425"
     CDS             6850..7101
                     /locus_tag="JQR05_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5325485.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7103..8770
                     /locus_tag="JQR05_003426"
                     /pseudo
     CDS             7103..8770
                     /locus_tag="JQR05_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2203 a         2278 c         2174 g         2115 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgac cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaatcagtt gtcatgcact
      541 cggaataaat cataccgtga atagtgctgt ttttgattgt cagactattg gcgttggtat
      601 tggtagaaat accgtcgtcc agatagtagg tagaaatcac gccgttaacg gtagcgttat
      661 taatgaccgg gtagatatcg ccgttgtaat agctatcagc ggcatagttg ttccagccta
      721 cataaccatc atagtaaacg tcatcagcat aggtggcgtc attgtagtga tggaaagtat
      781 tgtaagtttg accagaaata tcgttggtcg ctgcattagc ctgagaggtg atagccagag
      841 tgcaagccaa tgctaattgt gatactacaa gtttcttttt ccaggagtgc atttgtcatc
      901 cctcctcagg gacgtaataa gttgatccat caattatcaa tgcatagaaa acatattatg
      961 tttcgcgcta acgattatgc tgcgttacag acgaatggtt caattattct ttcccctaag
     1021 tccgaatgtg gacatatata atttatggag aaaaacacgc atcaaattga taaaaggacg
     1081 atatttcagc atgttgcaga taatgttatt tttgatacat acgcgttata aatatcaaaa
     1141 acacaaacca gataaatatc ttatcaatat acaacaaagt ttgaatcatt caatttatag
     1201 cagcacaacc aaacgccaaa aaataataac caagagctat aaataaagat taaaaatatt
     1261 ttcagtgaat atgaaattat tgctatttct attacaaaga tgtcctggtc tctctttcat
     1321 tctcttcttg tcaacatcgc gacaactttc gtaaaacact cctgccccgc ttcaggtata
     1381 ctgtgcacct taattccaca aacatcaggc agaccatgac agacttaatc caacgccctc
     1441 gtcgcctgcg caaatctcct gcgctgcgcg ctatgtttga agagacaaca cttaacctta
     1501 acgacctggt gttgccgatc tttgttgaag aagaaattga cgactacaaa gccgttgaag
     1561 ccatgccagg cgtgatgcgc attccagaga aacatctggc acgcgaaatt gaacgcatcg
     1621 ccaacgccgg tatccgttcc gtaatgactt ttggcatctc tcaccatacc gatgaaaccg
     1681 gcagcgacgc ctggcgggaa gatggactgg tggcgcgtat gtcgcgcatc tgcaagcaga
     1741 ctgtgccaga aatgatcgtc atgtcagaca cttgtttctg tgaatacact tctcacggtc
     1801 actgcggtgt gctgtgcgag catggcgtcg acaacgacgc gactctggaa aatttaggca
     1861 agcaagccgt ggttgcagct gctgcaggcg cagacttcat cgccccttcc gccgcgatgg
     1921 acggccaggt acaggcgatt cgtcaggcgc tggacgctgc aggctttaaa gatacggcga
     1981 ttatgtcgta ttcgaccaag ttcgcctctt ccttttatgg cccgttccgt gaagctgccg
     2041 gaagcgcatt aaaaggcgac cgcaaaagct atcagatgaa cccaatgaac cgtcgcgagg
     2101 cgattcgtga gtcactgctg gatgaagccc agggcgcaga ctgcctgatg gttaaacctg
     2161 ctggagcgta cctcgacatc gtgcgtgagc tgcgtgaacg tactgaattg ccgattggcg
     2221 cgtatcaggt gagcggtgag tacgcgatga ttaagttcgc cgcgctggcg ggtgctatag
     2281 atgaagagaa agtcgtgctc gaaagcttag gttcaattaa gcgtgcgggt gcggatctga
     2341 ttttcagcta ctttgcgctg gatttggctg agaagaagat tctgcgttaa tttttttcct
     2401 ctcagaaagg gggagttaac ctgttgaaag tacggtgaac gccccctcac cctaaccctc
     2461 tccccaaggg ggcgagggaa ccgtccgcac tcgtatcacc ccgcccgata aaacggttta
     2521 tccccaagga tcgtcgcccg atgcataatc cgccgctgtg gcaggtaatc ggcattggca
     2581 tagtgctgag tcacgcggtt atcccaaatc gcaatatcat tcggttgcca gcgccaacgc
     2641 acctgaaact ccggtttagt gatatgggcg aataaaaagc ctaacaaggc ttcgctctct
     2701 ttctcgctga catcgacaat tcgcgtagta aagccttcat tcacaaacag cgcctgttta
     2761 ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg gaggattttt cgcgaccgcc
     2821 tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt tgtattccgg gaatgattta
     2881 cggaaatcat gctccgcacg cagccagctc agcagctggc ggaagggaac agagagcgcc
     2941 tcataggccg caataccgct ggtccagagc gtatcaccgc cggtcgaagg taactcttta
     3001 gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg tcacatcggt atgccagttg
     3061 tcgttatccg gcggattatc gttatgggta tccagcacga tgatctcgtc aaccccttcg
     3121 gcatgagggt aaacagggtg aatatgcagt tcgccaaaac gctgggccag cgcgcgttgc
     3181 tgctgcggcg taatagcttg atcgcgcaga aataccacct gatggcgcag caccgcatgg
     3241 taaagctgtt caaactgatt atcgcttaac gggcgcgtca ggtcggcacc cgaaatttgt
     3301 gcgccgatat acggccccag cggggtaatg ctcagacgtt cactcattgt acttctccat
     3361 gccagggcgt cagacggcgc tgtaacgcgc gcagacccag ttctaaaaga aaggcgataa
     3421 tcgcaatcac cgcgatcccc gccagtacca cgtcagtcgc gagaaattca cccgctgact
     3481 gaaccataaa tcctaaaccg cgtgtcgcgg caatcagctc cgccgctacc agcgtagacc
     3541 agcccacacc cagaccaatg cgtaatccgg tgaggatttc cggcagcgcg ccgggcaaaa
     3601 tgacaaacca cagcacctgc gcacggctgg cacccagcga ctgggcggca cgaatgcgaa
     3661 cctgctgcgc gcttttcacc cctgccagcg ccgacatcgc caccggcgca aaaatcgcta
     3721 aatagatcag taagatcttc gaggtttcac caataccaaa ccagatcacc atcagcggca
     3781 aataagccag cggcggcacc ggacgataaa gttcgattac cggatccaga atgccgcgca
     3841 ccgtcgggct tagccccatg gcgatcccga ccggaatacc gatcaccacc gccgcgagta
     3901 gcgccagcac aatgcgcgtc aggctggctg ccagatgctg ccacagcgtg gcgtccataa
     3961 atccctgcgg tccggcaatg gtgagcagtt ttgccagtac ttgttgcggc ggcggcaaaa
     4021 acagcgggct aatcagttgc agcgccgcca ccgtccacca tacagtgagt aaaaccgcta
     4081 acgtgccaat gcttaaggtc acctgacgcg agagcggcca gcgccatttc agccgccgcg
     4141 aatgcagttt ttcattaatg agcacactca tgagaacgcc tcccgttgct caaatacgcg
     4201 gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg gacttgatgc tgcggctcga
     4261 ctctcccgca acaaagcggc gagcaaagtt gagcggcagc cgctccagca cacggccagg
     4321 gccggatgaa agcagaacca gttcagtcgc cataaacacc gcttcttcta tatcgtgggt
     4381 aatcaacagc acctgcttgc ccgtctcctg ccagagtttc agcagcagag tttgcatctg
     4441 gtcgcgggtg aaggcgtcca gcgcaccaaa cggttcgtcg agtaataaca gctggggatt
     4501 cgccgccagc gcgcgggcaa tccccacccg ctgacgttga ccatcggaaa gctgccagat
     4561 atagcgtttt tctgcgcctt ccagccccac ttttttcagc atctggtgcg cgatttccag
     4621 tcgctgcatt ttctctatac ctgccaattg caggccgaac gccacgttgt cctgtacatt
     4681 gcgccacggt agcagccctt cattctgaaa aactacgcca cgctctgctc ccggtccctc
     4741 aatacgctta cccgccagtt gaatgctgcc atgctgataa ggcacaaaac cggcaatcag
     4801 attcagcagg gtggttttac cgcagccgga cggccccagc accaccagta gctcgccgct
     4861 ttccagcgtc aggttgatat cttccagtgc cggtttgccg ccataatcgg catagagatg
     4921 agagatttgc agcatctgcg cctcctttta ttgcacgaag cgcgaggtaa cgtactggct
     4981 gtaatcattc gctacagccg ggaccttgcc ctgctctttc aaaaactgcg cggtgtcgat
     5041 gattgctttg ttcaccggtc cggtcagttc tgccgtttgt tgctgcggcg tcagataggt
     5101 atttcccttc accagccccg gaacgtcacc ttcaggcacg ccgcttaaac gcgccagttt
     5161 gctgatgttt tccggctgtt tcagccacgc gtcagggtta gcaatgtacg gttgctgtgc
     5221 atcgatggcg cttttggcga acgctttcac gacctcagga tgtttctcgg caaaatcttt
     5281 gcgcaccacc cagacgtcca gcgttggcgc accccactgc ccgacctgtt cagaatcggt
     5341 cagcaccttg ccgtcttttt ccagggcgtt aaccgccggt gcccagacat aagcaccatc
     5401 aatatcaccc cgctgccatg cggcgataat cgcgggcggc tgcaggttca caatctccac
     5461 tagccctggt ttaatccccc agtgtttcag cgccgccagc aggctgtagt gggtggtgga
     5521 gataaacggt acggcaatgc gtttaccaat caaatcttcg ggtttgctga tagttttctt
     5581 taccaccagc gcttcggagt tacccagttt tgacgccagc aagaagactt caatcggcac
     5641 ctgttggctg gctgcaaccg ctaatgggct ggaaccgagg ttgccgattt gcacgtcgcc
     5701 agaagccagc gcccgcacga tgctggctcc gctgtcaaac ttacgccagt ccacgctcgc
     5761 cccgctctct ttagcaaagg tgttgtcggc ctgagccact ttcgccggtt cggctgaggt
     5821 ttgatacgcc acggtgacgt tcaccgcctg tgcctgaaaa gcgatgaatg ccagtgcggc
     5881 aagaagtgtg ttatatgact aggtaattta ttcaagataa ccgggaatgg gtaaaaaaag
     5941 acgggatttg atgggattca ataagttatg cgccgattgc attcaagata aaacgagtta
     6001 aaaatgctca atgcttcgat atcgataaaa cattcaagat aggcgcgccg tgtattttaa
     6061 ttcttttaag actcttcaaa aatgtcatcg tagcctttaa aaacgaattt taaaggctac
     6121 cctaaaggca ggtcaatgaa gttaacccgc cttcgcgttt acactctctg agagaatact
     6181 agcttcaatc tcttccaaaa tctctctgac ttgctcgtca ggcatatcaa cgagcatcaa
     6241 cgctgccagg cgtttacagc cctctagttg cagtagctct tgagtttcct tatctaacaa
     6301 gcgtagcaca gtctcaacac ctttccttgc gagcatgttt gtaagggctt ctcgttcctc
     6361 actggtaaga aacgacaaca tttctgttag ctgatcccct tggcttaaat cactttgtgt
     6421 tttttgtgtt tttggtggat tcccgttacc ggtttttagc cattcaagac taacaccttc
     6481 actttctgca atgcgtacca atatgctttc tcgaggttct ggtgtatttc cctttttaaa
     6541 atagctttgt aacgtattga tatttatgcc ccaggctctg gcggcagcac ttcttgatgg
     6601 ataccgccta ataagataag ccatgcgctc cacgataggt tcttttgcct gctccataaa
     6661 agtacctcta ggtatttttc tgtataaagg ttcttttcca tctatctaat tgaatggaaa
     6721 ggtaatttta aaaatccaaa taaaatacaa aaaaatacct tgtattggtg ctttttgtga
     6781 tctgcgattt tatttactcg gatatccacg cgaatatccg cgcgggtaat tttgtagggt
     6841 aaaggaaaga tgagacaaag agaagatagt ggccgcgatt ggccttcgct gatgattcag
     6901 caagcactca aagataatgg gtggtcttcc tttcgggagc tttcactcca tgccggttat
     6961 gctcaggatt cactgaaagc ggtagttcgt acgccaaaca aaaaatacga acgtgttatt
     7021 tctgacgcta taggtgtacc gccagaaatc atctggccca gccggtatcg taacgatgga
     7081 tttatcagga aggcgtcgtg atatgcagtc gtacgttacc gtaaatgacc tgctcggtgt
     7141 tcctggaatg ccagccaccg cgaaaggcat ccgacaggcg ttacagcgtt tttctcgtgc
     7201 tcttggtgac gttagtcgcc gtcgagcagg tacaaaagcc attgagtacc atatcgattg
     7261 cctgcctgag gtaacccaaa aggcactgcg tgaacgctat gtggaacaac tggtggctac
     7321 agagaacaac gtctccgaag tgaagaccgt taccaggacg gcgcgtaacc ctgatgctgt
     7381 tcaggcagtt gaagcatatc ggggttctcc gcaactaata gaagaacgcc tgaatgccct
     7441 gactgaaaat cagcgccggg tgtctgaagc aagagctgcg ctggcagtgg aagtgctgaa
     7501 gctggaaagc gccggtaacc tcgggcgact gaaagccatt aactttctgg ttgaaaaagc
     7561 ccgtaaaggt gagttgccgg aacgactgca acaggctgcg attaacgcta atgcaaaacg
     7621 tggcactaat cgcaccatca gccgtgatcc gctttaccag tgggtactga aatacaaccg
     7681 gtcgcagaac gccgcagaac gactgttgtt actggcacca ggaaaacgcg acgaaataaa
     7741 accggaagaa attagctggc tgcctgaatt tctggcgcag tatcgtcagg ctaacggcag
     7801 acctatgtcg gaagcctacg aggattttgt cacggaatgg caacgtcgct atgcggacga
     7861 accgtacatg ctggaggtga tgccttccta tgatgttgtc cggtatgcca tgaaaaaatt
     7921 gccggaagtg gtgaaacaaa aaggacgcgt taccggcagt gaataccgcc agcttgaggg
     7981 attcacccgc cgcgactgga cagctatgcc ggtgaattat gtctggattg gtgacggcca
     8041 cggtatgaag ctgaaatgtg cgcatccgat ccacgggcgt ccattttcac cggaagtcac
     8101 gtttgttatc gacggtggaa cccggtttgt tgttggctgg agtcttgatc ttgcggagaa
     8161 cgtgtttgct gttgccgggg cgatacagca cggaattcgc aatcacggaa aacccttcct
     8221 gtattactcg gataatggtt ccggtgaaac ggcagatatg ctggataaag agatcgtcgg
     8281 gattctgccc cgtctgggta ttaaccaccc caccggtatt gccggaaatc cgcagggacg
     8341 cggcatcatt gaacgtctga accggacgct gccgatgcgt atagccagac ggtatcgcac
     8401 ctattttggc aagggggcag accgggaaac actgcgtgta ctcaatcgcg atctgcgttc
     8461 agcatttaat gccctgcaac aggacaaacc gctgaatgcc cgccagaaat ctgcaatgcg
     8521 cgagctgccg tcctgatcag aactgattga ggctattcgt gaaggagtgg aatggtacaa
     8581 caaccgcccg cattctgaac tgccgatgaa accaaacggg aagcattaca gcccggctga
     8641 gttcagaaaa aaacgcctgg cggaagaaaa caccgaaatt gaatggctga gtgatctcga
     8701 actgcgcgac atgttctgcc cgatggtgga gcgtccggta agacgctgtg aaatcaaatg
     8761 gcttgggaat
//
LOCUS       DADKXM010000150         8592 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.152, whole genome shotgun sequence.
ACCESSION   DADKXM010000150 DADKXM010000000
VERSION     DADKXM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.152"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..1859
                     /locus_tag="JQR05_003427"
                     /pseudo
     CDS             1..1859
                     /locus_tag="JQR05_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1828..3564)
                     /gene="aceK"
                     /locus_tag="JQR05_003428"
     CDS             complement(1828..3564)
                     /gene="aceK"
                     /locus_tag="JQR05_003428"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY5325486.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKWHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3748..5052)
                     /gene="aceA"
                     /locus_tag="JQR05_003429"
     CDS             complement(3748..5052)
                     /gene="aceA"
                     /locus_tag="JQR05_003429"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY5325487.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQLPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5082..6683)
                     /gene="aceB"
                     /locus_tag="JQR05_003430"
     CDS             complement(5082..6683)
                     /gene="aceB"
                     /locus_tag="JQR05_003430"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY5325488.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6952..7881)
                     /gene="metA"
                     /locus_tag="JQR05_003431"
     CDS             complement(6952..7881)
                     /gene="metA"
                     /locus_tag="JQR05_003431"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY5325489.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8038..8481
                     /locus_tag="JQR05_003432"
     CDS             8038..8481
                     /locus_tag="JQR05_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY5325490.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERVQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2206 a         2106 c         2111 g         2169 t
ORIGIN      
        1 aattttacct caacgctcag acactcttga taaacaagaa ttaaacctaa aatcagatag
       61 aaaagaaaaa gaattcccaa gaattaaact taatggtcaa tgttattttc cggggcgccc
      121 caaaaccata tagtataccg acacattgct gcacaatata ttaatgatat ttatcagaat
      181 gttgattaca aaccccatca agatgattac tcttcagctg aaaaatttct cactcacttc
      241 aacaaaaaat gcaaaaacca gactttggcg ttgatttcca gccgtcctga ggggcgttgc
      301 gttgctgcct gcggtgattt cgggctagtt atgaaagcat attttgacaa gatggaatca
      361 aatggcatca gtgttatggc agccatatta ctggtggata accatgcttt gacggtccgg
      421 ctaagaataa agaacacaac tgaaggatgt acccattacg tggtttcggt ttatgatcct
      481 aatgtaacta acgataaaat aagaattatg agcgaaagca aagaggatat taaacactat
      541 tctctgatgg attttatgaa tgtagattat agcctcctga aatggtcaaa tgatcatgtt
      601 attaaccaat ctgttgcaat aattccagca cttccgaaag aacagctatt gatgttaaaa
      661 ggaactgtgg atgaaataac ccctccatta tcaccagcaa cgatgaattt gctaatggca
      721 attggtcaga atcaccaact tacgcaactg atgattcagc tccagaaaat gccagaacta
      781 catagaacag aaatgttgac tgcctataat agtattaacc tccccggttt atatttggct
      841 ataaattatg gcaatgcgga tatcgttgag actattttca attcattgtc agaaacagga
      901 tatgaagggt tactctcgaa aaaaaatctc atgcatattc tggaggcaaa agataaaaat
      961 ggtttttctg gattattttt agcgatatca cgtaaggata aaaatgttgt aacctcgatt
     1021 ctgaacgtct tacctaaact ggccgcaaca catcatttag ataatgaaca ggtgtataaa
     1081 ttcctgagtg ccaaaaatag aacgtccagc catgttttat accatgttat ggcgaatggt
     1141 gatgccgaca tgctgaaaat tgttttggtc gcgttacctt tgttaattcg cacatgtcat
     1201 ttgactaaag aacaggtact cgatctcctg aaggcaaagg atttttatgg ttgcccaaga
     1261 ctatacctgg cgatgcaaaa tggacatagc gatatcgtga aagttattca cgaagcattg
     1321 ccctgtctgg cccaggaaat taacatttca gcttccgata ttgtagatct tctgaccgct
     1381 aaaagtcttg cgcgcgacac gggtttgttt atggccatgc agcgcggaca tatgaacgtt
     1441 attaatacta tttttaacgc attacccact ctttttaata cgtttaaatt cgataaaaaa
     1501 atatgaagcc cctcctcctg gcaaataatt ctaatgaata tcccggtttg ttttcagcga
     1561 tacagcataa acaacaaaac gttgtagaga cggtttatct tgctttatct gaccatgcac
     1621 gcctgtttgg atttaccgct gaagatatta tggatttttg gcaacacaaa gcgccacaaa
     1681 aatactctgc ctttgagttg gcttttgaat ttggtcaccg ggttattgct gaattaatcc
     1741 ttaatacatt aaataagatg gctgaaagct ttggctttac ggataaccct cgatacattg
     1801 cggagaaaaa ttatatggaa gctttactca aaaaagcatc tccccatacc gtacgctaaa
     1861 tctttgcctg cgccgatacg cataaacatc ttccacatgc ccttcacgta tgcggttttg
     1921 tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa acagcggacc
     1981 aatacgcggg tcggcgcata gccagtggcg aaactcttcc gggaaaacat cgcccggcga
     2041 gacgctgtac cacggttcgc tggcaagttc gtcttccgga tagcgcggcg gcgggatgtc
     2101 gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa ccacacgccc
     2161 gtgacgggtg acaccgaagt ttttaaacag catgtcgcca gggaaaatgt tagcagcggc
     2221 aagctggcga atagcgttac cgtattcttc aatggcgtcg cgcaactgct gaccttccac
     2281 ttgttccagc cagatattga gcggcaccat ccgccgctcg atatagagat ggcgaattac
     2341 aatctgctca ccaagatcgg tgattttttc cgctgcttcc tggagcagta attccattaa
     2401 taccggggaa atatgccact tctccagcac aaagttttca aactcctggg tgtccgccat
     2461 tcggcccaca cggtcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga
     2521 catctctttc tgcggcgcga acttgtcttt gatgactttg aatacccgat caaagcccgg
     2581 cagcgtaaac accaacatca ccattccacg aatacccggc gcttcaatga actgctcatt
     2641 acagccctgt agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca
     2701 gccgatagcc atatacaatt cagcagtggt tttacctggc agaatttccc gtagccactc
     2761 gaccagcgct gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa agccaaaaac
     2821 aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt
     2881 ctggtggatc ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt tgcccaccag
     2941 ccaggcggct ttattgcggt aaaacagttc gttcgccacc tgtaaatgac tttccgcgag
     3001 gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt cacggctttt
     3061 attctgccag cgcaggcgca gcggcaggtc gctgataacg cgcatcagta gagattccca
     3121 gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga atggtacgaa agcggcgctc
     3181 tggctgagag ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataaccg
     3241 acagtacacg gagttaaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag
     3301 ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt ggccgttagt
     3361 aatgcagcgc agttgctcca cgaccagacc aacgtgatga tcgtaaagat ggatacggtt
     3421 tttcatcgcc tgctggacag catgccagtc ggcctgttcg aaacgctgct gcgccccgga
     3481 agtcacttcg aggaatcgac catactgagc gtcgaagcct tgcaaaatgg tttgagcaat
     3541 cagtaattcc aggccacgcg gcatttagcg ccctcatcag gagcagagaa ttgccggatg
     3601 cggcgtaaac gccttatcca gcctacgttc ggcaacggtt gtaggcatga taagacgcgc
     3661 aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc cttatccggc
     3721 ctacagtcag caacgtttgt aggttgctta gaactgcgat tcttcagtgg agccagtcag
     3781 cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc actttatcga agtaacctgt
     3841 acccacttcc tgctggtgag atacgaaggt atagccatct ttcgcggcgg caaattccgg
     3901 cagctgcact ttctcaacat agtgcttcat accttcgccc tgcgcataag cgtttgccag
     3961 gtcgaacatg ttaaaccaca tgctgtggat acctgccagg gtgatgaact ggaacttgta
     4021 gcccatatcc gacagctgct gctggaagct ggcaatagtt ttgtcgtcga ggtttttctg
     4081 ccagttgaac gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat
     4141 agcttgtgca aagcgacgcg ccagttccag atccggcgtg gaggtttcac accagactag
     4201 gtcagcatat ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt
     4261 acggaagaag ccttcactgg tacgctcgcc ggtaataaat tcgctgtcat acgggtcgca
     4321 atctgaggtg atcagatccg ccgcatcagc atcggtacgt gcaaccagca gggttggaac
     4381 gcccgtcacg tcagctgcca gacgcgccgc gaccagtttc tgaatagctt cctgagttgg
     4441 cactaaaact ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa
     4501 gtgaactgcc gcagcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac
     4561 accgccaaaa ccggcttccg catcggcaac gatcggcagg aagtaatcga catagcgcgg
     4621 atcgcccggc tcaatgccca cggaccattg gatctgatcg gcacgacgga aggtgttgtt
     4681 gatccgctcc accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat
     4741 gctggccgcc aggttagcgt ccgccgctac ctgccatccc gacagataga ctgcttcaat
     4801 acccgctttc gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc tgttgatgta
     4861 gccttttttc gactcaccgt gcagcagacg ccacattttc gctgcgccca gttgcgccag
     4921 cgtgcattca ggattgactg aaccgcgtaa tttcaccaca tcttccgcac tgtatgggcg
     4981 agtaatgcct tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt
     5041 acgggttttc atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag
     5101 cctggcaggg tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt
     5161 gcggcatcgt caaaacggcc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact
     5221 ttcatctctt cgcccagcat ctggcggaac aaggctttgg tcaccggttt gccattgctc
     5281 aacgtttttt gatgatggat ccactgccag atcgaggtac gggaaatttc agccgtcgcc
     5341 gcatcttcca tcaggccata aatcggcacg cagccgttgc cggagatcca cgcttcgatg
     5401 tactgcacag ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc accatcacaa
     5461 ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt cgcgcaatac ttcaagctga
     5521 tttttacggg agccgagaat gtcgttgaat accgccattg ccgtgtccgc aaggcctggg
     5581 tgagcgatcc atgtgccatc gtgaccgtta ttggcttcca gcgctttatc cgcttttact
     5641 ttgttgagca cctggttatt acgctcttca tctttgctcg gaataaacgc tgccatgccg
     5701 cccatcgcaa aagcaccgcg tttatggcag gttttaatca gcaggcgtga gtaagcattc
     5761 aggaatggtt tatccatcgt cacggcctgt ctgtctggca ggacacgatc gggatagttt
     5821 ttcaacgttt tgatatagct gaagatgtaa tcccagcgac cgcagttcag accaacaata
     5881 tggtcgcgca gcgcgtgaag gatttcgtcc atctggaaca cggcgggcag cgtttcaatc
     5941 agcaacgtcg ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag
     6001 acttcgctcc accaggccgc ttcctgccag gactgggttt tcggcagata gaaataggga
     6061 ccgctgccct ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac
     6121 aggctaccgg ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg
     6181 cgtacccgac aaatcaaaac cgctggattg ggcttgagct gataaatttt gcctgcttca
     6241 ttggtatagc tgatggtacc gttaaccgca tcgcgcaggt taatttgccc gtcgatcact
     6301 ttgttccagt ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg
     6361 ttcagtgcgt tgatcaccat cttgcgctct accgggccag ttatctctac gcggcggtct
     6421 tctaagtccg caggaatccc gcgaattttc caatcagcat cgcgaatgga agccgtttcc
     6481 gaaataaaat caggcaacgt tccgttatca atatcttgct gctgctgaat gcgcgctgcc
     6541 agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct
     6601 tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc cagttcatcg
     6661 gttgttgttg cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccct
     6721 ctgtgaacga aggaacgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa
     6781 atgcaaaatc aaaaacaatt tccattttaa ttaaaaatac ttgataactt attgataaca
     6841 atatgaataa aatttattca cttgctgagg tgcgtttcgg aaataaaaaa ggcacccgaa
     6901 ggtgcctgag gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag
     6961 cgttggattc atgtgccgta gatcgtatgg cgtgatctga tagacgtaat agttgagcca
     7021 gttggtaaac agcaaattac cgtgactacg ccagctcgct cgcggtttgt tttgcggatc
     7081 gttgtgcggg aaatagttat acggtacatc cgggtctagt ccggcttcca catcgcggaa
     7141 atattcctgc gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg
     7201 cttatctttg ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag
     7261 atcggtgtaa tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gcggtgccag
     7321 gaatgaatca tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta
     7381 aacgccagag agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc
     7441 ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag
     7501 cacctgtttg atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc
     7561 accggttaca atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa
     7621 gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg
     7681 aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat
     7741 cttcttcggc atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc
     7801 acgagaagtt gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc
     7861 gtccggcaca cgaatcggca taacctgatt acctcactac atacgcttat acgtttagac
     7921 atccagatag ctaaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg
     7981 agaaagtttc aaggaaaagg ctacgttaga atataagaat gacgaaaagg agagaaaatg
     8041 gttattagta ttcgccgctc acggcatgag gaaggggagg aactcgttgc gatttggtgt
     8101 cgatctgtcg atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag
     8161 ctggtgcgtt cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggtgcag
     8221 ccggttgggt ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat
     8281 gtgtgcggct gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa
     8341 ctgacaacca acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt
     8401 tttaaggtta cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat
     8461 ctggcgtatg tgggggaata agccgcccct gccgtgcctg cccaagctgg agaagacgcg
     8521 ccagcgtcgc acccggcatt tttttcacca acgcaaaaag gccatcctga cggatggcct
     8581 ttcgcttaat tt
//
LOCUS       DADKXM010000151         8469 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.153, whole genome shotgun sequence.
ACCESSION   DADKXM010000151 DADKXM010000000
VERSION     DADKXM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.153"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            299..628
                     /locus_tag="JQR05_003433"
     CDS             299..628
                     /locus_tag="JQR05_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY5325491.1"
                     /translation="MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            618..926
                     /locus_tag="JQR05_003434"
     CDS             618..926
                     /locus_tag="JQR05_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325492.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(968..1144)
                     /locus_tag="JQR05_003435"
     CDS             complement(968..1144)
                     /locus_tag="JQR05_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY5325493.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKCKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(1141..1668)
                     /locus_tag="JQR05_003436"
     CDS             complement(1141..1668)
                     /locus_tag="JQR05_003436"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY5325494.1"
                     /translation="MTIKSNTPAHDKDCWQTLLWLIDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(1665..2105)
                     /locus_tag="JQR05_003437"
     CDS             complement(1665..2105)
                     /locus_tag="JQR05_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY5325495.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(2183..4058)
                     /locus_tag="JQR05_003438"
                     /pseudo
     CDS             complement(2183..4058)
                     /locus_tag="JQR05_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/helicase"
     gene            complement(4288..5064)
                     /locus_tag="JQR05_003439"
     CDS             complement(4288..5064)
                     /locus_tag="JQR05_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5325496.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLILIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKRECRRKGELNQSNVFYLTLDNAQQIPPESGGAGA
                     ARGGAGAALGGGAGAAPRTYHSFEPVNEPKNIMFEHVRTECEKTPDRHEETDKAFEEI
                     FWCAGMRKAGKKNAASAFRTQFREWRKTTRGTASEFATMLADDIACRNGKQFGFDRLL
                     PSSYLNGQRWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
     gene            complement(5227..5526)
                     /locus_tag="JQR05_003440"
     CDS             complement(5227..5526)
                     /locus_tag="JQR05_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325497.1"
                     /translation="MTQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYAIDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(5665..5889)
                     /locus_tag="JQR05_003441"
     CDS             complement(5665..5889)
                     /locus_tag="JQR05_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5325498.1"
                     /translation="MNLVIQRALKIAGSQKRLADKCGVTQPAVHKWLKGGQVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            6034..6708
                     /locus_tag="JQR05_003442"
     CDS             6034..6708
                     /locus_tag="JQR05_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325499.1"
                     /translation="MRSAGVSQSQLADMVGISQPAIQKMSSGKTNGSRKMVELANALK
                     VRPEWLSSGIGEMRDGTHEEPSNIRESSLKAVVWEDIKKNDDEFVALPLLNVSLSAGS
                     GSCEIEESSEFSLVFRKYYLKKMGVTESSAKLVRVTGQSMEPTLHDGDVVGVNTQDTT
                     IRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEFHKTVRIIGRVF
                     WSSHSW"
     gene            6746..7087
                     /locus_tag="JQR05_003443"
     CDS             6746..7087
                     /locus_tag="JQR05_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HAY5325500.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            7619..7810
                     /locus_tag="JQR05_003444"
     CDS             7619..7810
                     /locus_tag="JQR05_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325501.1"
                     /translation="MAPKKKAVADVQVGVKTFELPIPLRGDLIVTIGNLPRDLTKDEA
                     KRISLIVESFAMIDGGTKE"
BASE COUNT         2146 a         2125 c         1909 g         2289 t
ORIGIN      
        1 cattatcgcc accttctggt ctcaatcatc tggtggcgag ctgtgcaggg ttgagactac
       61 cgggtacgga aaccggcgag cctttcggct cccccacaca gcccaccata aatcgcgaat
      121 gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct ccgtaaacat
      181 ccggggtctc aatcccgacg ccagattttg ctggcgtatg aggaatatag ccccggatat
      241 gtgttgtcgt caactacagg ccttgaatgc atataacgat tttgttatgt tgatacctat
      301 gaactacact atcgaatact acagtgaaga tgtaaggctg gaagtcgatc ggcttccatt
      361 gagtatgcgt gcccgatacc aacatctcgt tgaacgtatg aagatatatg gcagcaatct
      421 cggagaacct cacaccagcg cctttggtga cgggcttttc gaactcagaa ttaaaggcaa
      481 cgatgggatc gcgcgtgttt tttactgcac tctgacaggg aaacgcatca tcatgctgca
      541 tagttttgta aagaaaacgc agaaaacacc gccagccgaa cgcaagaaag ctgaaaccag
      601 aatgaaggag gttaagcatg actggtaaac gcactctccc caccatgaca cacgacgaaa
      661 tggcagccaa atggatggaa gacccggctt ttaaagcaga atacgacgct atcgctgacg
      721 aattcgcact gcttgatgaa atgctggcag cacgcaaaga agctggctta actcaggctg
      781 aagttgccga gcgaatggga acaaaagcga ccgcgatcac cagaatggaa agtaatctcg
      841 catcaggtat cagcggccca tcatttgcca ccctaaaaaa attcgcccgc gctactggaa
      901 aaaaactcca gatccgcttc gtttaaccac accgcgccgt cattctggcg gcgcaccgga
      961 taaatagtca tttcctcgca cgatgtctta gccaccggat atcccacagg tgagccgtgt
     1021 agttgaaggt ttttacgtca gattcttttg ggattggctt gcatttattt ctggagcgtt
     1081 tcgttggaag gtatttgcag ttttcacaga tgatgtcggt gaaacttcgt cgctgtcgcc
     1141 tcatgctgcc ctcctgacgc cctgcccgat cgccatcaat gccgctttgg atacggtagt
     1201 aaacatccgt cgaggactga tgaacggtcg ccaaatcagc agcatggagc ctttgctgtt
     1261 gcccttcttc tccagccctg tcgatggttc gataaaatta atccgtccat cagtgataat
     1321 gcgaacttcg tcgacactct ccagagcctt gctgaaccat ccgactgaca tatcctctgg
     1381 cacaagcata actaccgtct gtcgctgttg tatgcactgc tcagcggctt tttccaccca
     1441 cggcctgata ttgctgtacg gtgggttatt ccagattgct ccgtggctta cccactcaga
     1501 attgagcgcg tcgtcggcct cagttagcca gtgagcgcac agagcatttt tgtcgctcgc
     1561 tgccgaatcc agccagaatc caaactcaat atccagcgca tcaataagcc aaagtagcgt
     1621 ttgccagcag tccttgtcgt gtgctggcgt atttgatttg atagtcatgc agcccgatct
     1681 ccccatcgcg ctttccactc cagagccagt cgcgcttcgt ctgaccactt aacgccacgc
     1741 tctgtaccga atgcctgtat aagctctaat agctccgcaa attcgcctac acgcatcctg
     1801 ctggttgact ggcctattac cacaaagcca ttcccggcaa ggttaggaac aacgtcctgc
     1861 tgctttaatg ctgcggtaaa cacacacttc cagctttctg catccagcca gcgcccatgc
     1921 cattcaacct gacgagagac gtcaccaagg caagcccaaa gctttcgatt ctggtctaag
     1981 ctgcggttgc gctcctgaat ggttactacg attggtttgg ttgggtctgg aagaatttgc
     2041 tgtaccgcgt gaatagcgtt ttgctgatgt gctggagatc gaatttcaaa ggttagtttt
     2101 ttcatgactt ccctctcccc caaataaaaa ggcctgcgat taccagcagg cctgttacaa
     2161 gctcagtgat gtagatggtc atttaatact ccgtcacgtt ttcctgtcgc cacgcctcgt
     2221 catattccga tttcggcata ttggcgatgt agctatatgg cgatcctgat tcaagttgca
     2281 ggaactggtg cgattgctcg tcaaggaaca acgggacacc accttcccaa ccttcgcagt
     2341 tacgttgttt ttcaagcatc aaaacagatg ccggagatgc cagtagctgt tcgtccttct
     2401 ctgacatctt ttcaccactc tgaactctct gtaacgctct ctcgcgagtc ttgttacgcc
     2461 agatgatgaa aaggttgtct gtcaggtctg ttatcgctcc agagcctttt acgtccattt
     2521 tcccggttgg tttttcttcg ctgtctcctt ttcgcgagtg agtaacgaga atgacgtggg
     2581 agtttgtttt gtttttgaag tcgcaaatcg agtcaacaaa cgccttctgc ccgttatagt
     2641 catcgtcgcc tatgccacat ttcatcaggc tgtcgatgat gaataactgg atcccgtatc
     2701 ggcggcgagc gtagtcgaat atttcgatca gcctgtcggc tttcgccgtt ccggtcaggc
     2761 caaacaccca aagtctttcg tcataaaatt taaatgcaga gtcaatttcc agcactggcg
     2821 gaatcttgca gcacgtcgcc tgacgggtaa ggcgcttaag gagaatacca ggcttcagct
     2881 caagtgacgc gatgcacgtc ttcacaccct gacgcattgc ctcaagtgcc atatgcccga
     2941 caacctccgt ttttccgtga ccgttcacac cattgaccag cgtcaactct gcctcacgga
     3001 actagaattt atctgccaga gattcccacg gtggattaaa cagatactgc tgcttgccgt
     3061 agaaagcgtt gatagtgtcc tggtaaaacc gcactgtaga gttcttcagg atcgaagtag
     3121 gatgccgtgc cgatgtactg ccagatttca tcctcggtaa caccgttcat caggcattcg
     3181 ttgatgtctt tgtacggcag agtaacaaga cggcaacgat gttcaccgag tcggcttgcg
     3241 atttcccttg cggcttcacg accaacatca tcaacgtcca tcgagatgaa tatttcctca
     3301 aacctgtcga ggttgtgata ctcaaactca atccactgtt gcttagcgcc tttcccgcca
     3361 ccaaacggca cggataacgc cgagatgccg tattgcgcat agctcataca atcaatttcg
     3421 ccttcgcaaa gcacaaccgc cctcacgcca gcgtccagag cctgccatcc gaacaggcaa
     3481 ggttcgcaat caccttctgc cataatgact ttcttcccgt ccgggcgctc agtgctgatt
     3541 cgcttgacct gcaacaactc accatcgcgt ttgtacggaa gcaccagagc atccagttct
     3601 cgttctccat tccacacctt gccgctgaca acctcgtagc gctttacgac ttctggcgat
     3661 atgccacgcg attgcaggta ctcaagatgg gattctgttc tggtaacgta gtgggcgatt
     3721 ttcttgcgat caggtctgga gaatttcttc tcacgtttgg catcgaaatg gtgatcgtca
     3781 tccttgatac cgagaaatgc tttcgcttcc tgcatagcct gatgcaggtt aattccacga
     3841 catgccatcc acaaatcaag catgtcaccg ccgtctccct cagcgaaatc agcccatttt
     3901 ttcttaccgc taaggttgac cttaaggctg tttcccttgt caccgttgac gttaccggca
     3961 acccactcat gcccctcttt cttgccgttt ggcaacaggt gcggagccac cctgtcaacc
     4021 tgcgcccaaa gcaggtcgct aagttctgat ggactcatac tttaaccgtc ctgaatcgca
     4081 ttctgacgca atatttttta tcccgttatt ttcccgtcag accgtcgttt atcgcgctac
     4141 agcgcgtctg atggcgttta aacgccattg acggagctaa tggctgctga ggtcgagatt
     4201 tttaaaccag aaatcgacaa acgaaatact taaccagccg tggttataac cagcgaccag
     4261 tagcgatttg atttttgatt tcatggttca cctgtcgaaa aacacgtagc cagttttcga
     4321 tacggtaatt gcggatgatg gtctggattg tggttgaatg gtttctggct tctcgtcgtt
     4381 ccagcgctga ccgttcaggt agctcgacgg taacaacctg tcgaatccga actgcttacc
     4441 attcctgcat gcaatatcgt ctgccagcat cgtggcaaac tcgcttgccg tacccctggt
     4501 agttttacgc cattccctga actgtgttct gaatgccgaa gctgcgtttt tcttcccggc
     4561 tttccgcatg cctgcacacc agaatatttc ctcgaatgcc ttgtcggttt cttcgtgacg
     4621 gtcaggagtt ttttcacact ccgttcggac atgttcgaac ataatgtttt taggttcatt
     4681 gactggttca aaagagtgat aggttctggg ggcagctcct gccccacccc ctagggcagc
     4741 tcctgcccca ccccgggcag ctcctgcccc acctgattct ggtgggattt gttgtgcatt
     4801 atccagcgtc agataaaaaa cgtttgactg gttaagctct ccttttcttc tgcattccct
     4861 tttcaaaagc cccatctctt ccagtgccct aatgtgactt tttactgtcg atctgctcac
     4921 ctcacactgg tcagcgacat gttgatatga aggccagcat tcgccattat cattggcgtt
     4981 atcggcaagt ttaatcagaa tcagttttct cagtgggttg ccaaccttta tattcatggc
     5041 cttagccata agattcatgc tcattttgac ttctccgaag ttttgtacct gttaagtatt
     5101 tctctcagtg gcacagctat ttctggatta accccctgat aaaactggtc acgtagcaca
     5161 tcttttcggt gattaacgcg tttattttcc tgcgtttttc gcatataatt acctcgttgg
     5221 atgttattaa aattccatct ggatttgttc agaacgctcg gtcttgcaca ccgggcgttt
     5281 tttattggtg agtccatcaa tcgcatactt aaaagccctg ctaatcggac tgatgtctga
     5341 tgccattccg aaagcacaca agaccgaagc aataaacctc cagtccgttc tgcttatctt
     5401 cgattcatga cagccaatca tctttgccag accgcgctgg gtaagcgttg acaggttgat
     5461 gagtaaatct gtttcagcgc gatcaacgtc gcgctgtgat agtttgctgt aacttgtttg
     5521 tgtcatttct taatatttcc agtagtgaat agttagttga aaggtatgca tggaaacgca
     5581 tatggcctta gttggtcaga tatcttgggg ctcgctttgt cagcgacgta ggacgaatgc
     5641 ccattgtgaa aatagcggtg ttacttatgc tgcctgattc ggttttggaa acaggtgtgg
     5701 taaatctggg cgaatttcgt aagccttgat ctgccctcca gtggcgttaa cgatggcggt
     5761 aactttctct ggagaaacct gcccgccttt cagccatttg tgtactgctg gctgcgttac
     5821 accacacttg tcggcaagcc gcttttgact accggcaatc ttcaaggctc gttgaattac
     5881 taaattcatg agcatacctc tttcagccat tacttataac caaggataac ccaagttata
     5941 aaagatggca ataaccttta ttattttact ttggataacc gtagttatag atttgtggat
     6001 atgaaaacat tcgcagaaag actaaatgca gccatgagat cggcaggggt gtcgcaatca
     6061 cagcttgcgg acatggttgg aatatctcag ccagccatac agaagatgtc atccggtaaa
     6121 acaaacggat ctcgcaagat ggttgagtta gctaatgctt taaaagtacg ccctgaatgg
     6181 cttagttctg gtattggtga aatgagggat ggtacacatg aagaaccatc caatatccgt
     6241 gagtcatctt taaaagctgt ggtatgggaa gacattaaaa aaaacgatga cgagtttgtc
     6301 gcattgcctc ttcttaacgt ttcgctttcg gctggaagcg gtagctgcga gatagaagaa
     6361 tcatcagagt tctctttggt tttcagaaag tactatctga aaaagatggg ggtaactgaa
     6421 agttctgcca agctggtaag ggtaacaggt caaagcatgg aaccaactct tcacgatggc
     6481 gatgttgttg gtgttaacac acaagatacc accatcagag atggtaaaac ctacgctatt
     6541 tgccagtctg atttgttgcg agtaaaaaca ttaatcgcca ctcctacatc ggtgataatc
     6601 agatcaataa atcgcgaaga gtacccggat gaagtaatgg atagagatga atttcataaa
     6661 accgtaagga ttattggcag agtattctgg tcatcgcata gctggtaacc agtggcctga
     6721 agaaacgttt gggtgaggag gatagatggc gttcactgac cttgaatatc aagcggtcaa
     6781 aaaagaagtt caccaattca ttgaaagcat aaggccgcct gaacatatcc gcaatgaact
     6841 ggatattgtt tatagcatca atgaccaaac gatagatatc ggcgaacagc gccccgtgtg
     6901 gcagggcaac ccaggtgaaa caaacatcct gccatcagca agaatcaagt acatacgttc
     6961 tctggataga tggaaaatct attggatgcg gaaggatatg aaatggcatc agtacagtac
     7021 tgaactttcg ctgactgatg cgcttgagct tgtgcgtgct gacccggatt gctgcttctt
     7081 cggatgagtg aagagacatt ttggtgatgg atggtcgcag aagtgcggcc tttaatgtaa
     7141 tcgaagaaaa gtaatccttg taaaacttga tttgactgta ttgtcacaaa gaaaggaatc
     7201 aacgatcgtt tcttgggtct tgatttgttt tgacgatcaa attagtattg cctcatcaac
     7261 tgtatgaggc ttttttatgc aaaccaaaag ctacaccatc aaagcatttc aggacttctt
     7321 acagatgctt cctgagcttg gtatcgtcaa tgatgctaca gcccgcaatc tccgtgactc
     7381 ttccctgcgc cttcttactg ttcttgggtc ggatttcacc gatgccgata tccgtgatta
     7441 tagcgttgcc aacttagcaa gttcgtacgc agactcagca gaaagcaaac cctctgaatc
     7501 atctttgcag gcatacaaaa gccgaatgca aagtgctatt gataaattta tctcctacca
     7561 gaatggtgag attaatgtgt cagttggtga tgtaaataaa aaaaaggagc gtaaaaagat
     7621 ggcacctaag aagaaggcgg tagctgacgt acaggttggt gtaaaaacat ttgaactgcc
     7681 gattccattg cgcggagatc ttatcgtcac cattggcaac ctgcctcgcg acctgacaaa
     7741 agatgaagcc aagcgtatca gtctgattgt ggaatctttt gctatgattg atggcggaac
     7801 aaaagaataa agccccgcgg caacggggct tttggggtcg gaaatatttc cagtatttcc
     7861 gaagtgtatg gaggattacc taccgctaca cccttggcaa ccgcaagcca aggatagcgg
     7921 ttctcccaac aaactacaac ctgttcgggt aaacaggctg tatacaaaaa caggagaaaa
     7981 gctatggctc acgatgttaa tcgcgatgac ttcatctacg aacacgacaa gccggtttat
     8041 gtgcgcagtt actgccgcat acgtttcggt gagctcgaac atgttcgtca gcacttccgc
     8101 tcttatccga gctgctgaaa ataactaaat ctcaaacccg gcttccgcgc cgggttttct
     8161 ttgcctcgcg atcgtccccc atatttaaca gccgcaaatg cggtaaacca cgaaccaaca
     8221 cgccagcaat acagcgcccc ccttccaata ccgaccaatc cgcaacattt acaaaaataa
     8281 aataccttta ttatccatca cttataactt atttatcaga atccataaat taagttattg
     8341 accaccgcta taacctaggt tatctttaag ccatcagcag gacgcactac tcaccagggc
     8401 ggtgaatata cagcgattcg aatatgaatc tacggcgctg acaaagcgca ataaccaaag
     8461 tgaactttg
//
LOCUS       DADKXM010000152         8365 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.154, whole genome shotgun sequence.
ACCESSION   DADKXM010000152 DADKXM010000000
VERSION     DADKXM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.154"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..66
                     /locus_tag="JQR05_003445"
     CDS             <1..66
                     /locus_tag="JQR05_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yecR-like lipofamily protein"
                     /protein_id="HAY5325502.1"
                     /translation="CLNTEFTLSYQCHHSAFPVFL"
     gene            237..734
                     /gene="ftnA"
                     /locus_tag="JQR05_003446"
     CDS             237..734
                     /gene="ftnA"
                     /locus_tag="JQR05_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY5325503.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(771..1010)
                     /locus_tag="JQR05_003447"
     CDS             complement(771..1010)
                     /locus_tag="JQR05_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY5325504.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1200..2411
                     /gene="tyrP"
                     /locus_tag="JQR05_003448"
     CDS             1200..2411
                     /gene="tyrP"
                     /locus_tag="JQR05_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY5325505.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2473..3138)
                     /gene="yecA"
                     /locus_tag="JQR05_003449"
     CDS             complement(2473..3138)
                     /gene="yecA"
                     /locus_tag="JQR05_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY5325506.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3334..3420)
                     /locus_tag="JQR05_003450"
     tRNA            complement(3334..3420)
                     /locus_tag="JQR05_003450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3384..3386),aa:Leu,seq:taa)
     gene            complement(3433..3506)
                     /locus_tag="JQR05_003451"
     tRNA            complement(3433..3506)
                     /locus_tag="JQR05_003451"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3472..3474),aa:Cys,seq:gca)
     gene            complement(3561..3636)
                     /locus_tag="JQR05_003452"
     tRNA            complement(3561..3636)
                     /locus_tag="JQR05_003452"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3601..3603),aa:Gly,seq:gcc)
     gene            complement(3788..4336)
                     /gene="pgsA"
                     /locus_tag="JQR05_003453"
     CDS             complement(3788..4336)
                     /gene="pgsA"
                     /locus_tag="JQR05_003453"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY5325507.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4393..6225)
                     /gene="uvrC"
                     /locus_tag="JQR05_003454"
     CDS             complement(4393..6225)
                     /gene="uvrC"
                     /locus_tag="JQR05_003454"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY5325508.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHSKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKTGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6222..6878)
                     /gene="uvrY"
                     /locus_tag="JQR05_003455"
     CDS             complement(6222..6878)
                     /gene="uvrY"
                     /locus_tag="JQR05_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY5325509.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7174..7350
                     /locus_tag="JQR05_003456"
     CDS             7174..7350
                     /locus_tag="JQR05_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325510.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7337..7561
                     /locus_tag="JQR05_003457"
     CDS             7337..7561
                     /locus_tag="JQR05_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY5325511.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7594..8214)
                     /gene="sdiA"
                     /locus_tag="JQR05_003458"
                     /pseudo
     CDS             complement(7594..8214)
                     /gene="sdiA"
                     /locus_tag="JQR05_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT         2109 a         2116 c         1911 g         2229 t
ORIGIN      
        1 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
       61 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
      121 tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg caaataataa
      181 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
      241 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctagaactg tactcttcac
      301 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
      361 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
      421 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
      481 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
      541 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcagt
      601 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
      661 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
      721 acacacaaaa ctaatgctct ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg
      781 caaatcttac tctgatcggt cgaaaaacct tcttctgaag gtataaattt gccttttgct
      841 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt
      901 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
      961 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
     1021 caaaaaaagc ctagcgtggc gattacctct tatgaagact ttgcgccagc gcaggactga
     1081 atgcttttat tgtacattta tatttacaca atatgtaacg tcggtttgac gaagcagccg
     1141 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
     1201 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
     1261 tgctggcaat gccgctggct gcggccggtg ttggttttag cgttacgtta atcttgttga
     1321 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
     1381 ttccggcaga taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
     1441 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg
     1501 gtgccggtga attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa
     1561 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc
     1621 tggtcgattt atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc
     1681 tggtattgct gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
     1741 ggctggcact gtctgcaatc ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc
     1801 cgagtattgt cagctatatg gatggcaaca ttcgtaagct acgctgggtg tttataatcg
     1861 gtagtgccat ccccctggtg gcatatattt tctggcaggt ggcgacgctt ggcagcattg
     1921 attcaacaac ctttatggga ttgctggcta atcatgctgg attaaacggg ctgttacagg
     1981 cgttacgcga aatggtggcc tctccgcatg ttgagctggc ggtacattta tttgctgatt
     2041 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
     2101 tgtttcagcg ttcaaatacc gttggtggac gattgcaaac tggtgcaatt acctttctgc
     2161 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
     2221 gtgtggcgct ggcggtactg gcattgatta tcccttcact attgacctgg caaagcagaa
     2281 agcacaatcc tcaggcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
     2341 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
     2401 aagtggggtg atcagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
     2461 caatgtgacc tcttaatgca ggcagcactg cttaaatttc ttaccactgc cgcacggaca
     2521 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg
     2581 cggatgcgcc atccagtatg catgtaaatc aagcgccgcc agtcgaatgg catctacgct
     2641 ctcttcaaac gcttctggcg acattttctc tacccgctcg aagttttcct cagtaccgtg
     2701 cagcgcaatc gcctccagcg ctggttttaa agaatcgggc aacgttgacc agtcagaaag
     2761 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
     2821 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
     2881 ggtatcggcc atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc
     2941 ccagcgcggc acatagtcag ccccacccca cacggcaacc agccactgtt ctggttcaat
     3001 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
     3061 ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
     3121 taacggtccc gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta
     3181 catgccggtc cgtatcagag atactttttg agtggctttg ccggtgatta aaaattaagg
     3241 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg
     3301 cttattactt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
     3361 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
     3421 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
     3481 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt
     3541 gacgccgatg tcttttaaac tggagcggga aacgagactc gaactcgaga ccccgacctt
     3601 ggcaaggtcg tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca
     3661 cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg atgcgctgca ttctacttac
     3721 ctggcgcgat gagtcaacga tatttttcac cacttttgat cgtttgctga aaattacgcc
     3781 gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc
     3841 acagagtcag taccgcagcc acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt
     3901 tcggacgcca cagcagccag gccaacgcca ccatctgggc agtggttttc actttcccaa
     3961 tccaggagac ggccacgcta ctgcgtttac ccaactccgc catccattcg cgtagcgcag
     4021 aaataataat ttcacgggcg atcatcgttg ccgccggtaa tgtcacccac cagctgtgat
     4081 aatgctcggt taccagcacc atggcgatag ccacgagaac tttatctgcc acagggtcaa
     4141 ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt
     4201 cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca
     4261 gataaaagac caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag
     4321 ggatattaaa ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt
     4381 gctacagaga catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat
     4441 acccggcact tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat
     4501 atatttcaac aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact
     4561 ggtatttttg accttcgccc gttttttacg gtgcccgcca atcgcgtgat catgtgattc
     4621 atcgcgaata tgctggataa catgcagcgc gggtgaatct ggcggcaaac taaatccctc
     4681 accttccggc tcaaagaaca gcgtttccag tccagtctta cgatctgctc ctttggcaac
     4741 gccaagtagc agcggatgat ttttatccca tgagacatcc agttcggcga agacattttt
     4801 cgcctgcgca agctggcctt tgccgccgtc gataaggatc acatccggga tcttactgtc
     4861 gtcaatggct ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc
     4921 cggcgtgatg cctgtaatgt tatagcgccg atactccgca cgcagcgggc cgttagcatc
     4981 aaacaccaca caggaagcga cggtttgttc gcccatggta tggctgatgt caaagcactc
     5041 catccgcttc acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac
     5101 ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt
     5161 cagataacgc gccctatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag
     5221 ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc
     5281 acccggtaag gtgcgcatct ggctgccttg taaatagaac tgacctacga aggtttctac
     5341 cacctcgctc agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac
     5401 tttgccctga cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc
     5461 aataacgtca aggtcgtcgc cggtattgga aacaaactgt ttttcggtga cgcgtcgcac
     5521 cgcctgaatt tggtcgcgaa tacatgccgc ttcttcaaac tccagattct ggctggcagt
     5581 ttccatacgg ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg
     5641 cacatactcg acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc
     5701 cagacagcgc cctatctggt attgcagaca cggacgcgag cgattgcgat aaacactatt
     5761 ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc agtgtttcac gtacggcata
     5821 gccattcggg aacgggccga aatattcacc tttggaatgc tttgcaccac gatgcatcgc
     5881 cagacgcggg tgggtatcgc cactcaggaa gataaaagga taagatttat catcgcgtag
     5941 caaaacgttg taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc
     6001 ggtttctgta tgcgtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt
     6061 tttgcgcgaa gcgaggttgc tgcggaaata gctggaaagc cgttttttca ggtctttcgc
     6121 tttgccgaca tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggct
     6181 ggttacggtt tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc
     6241 tccgcattac acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg
     6301 tttagtttac tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc
     6361 tgttctgaga tctcattgac cttctggccc ttagtgatca tcagcataat ctgcaattca
     6421 cgttcagaca aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc
     6481 atttgttgag cgatatcaga agcaatgtaa cgctgcccag aatagacaga acgaatcgca
     6541 ctcacgactt cctgcggagc cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg
     6601 actttcgctg gtaaagggtt ttctgtatgg accgtaagca tgatgatttt aacatcagct
     6661 gtggaacgcg cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc
     6721 attagcacca cgtcaacagc atttgtccgg caccacttaa cggcgtcttc accgcacgat
     6781 gcctctccga cgacttttat accctttata tcttccagaa tgcgtcgtat ccctgcgcgc
     6841 accagttcgt ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg
     6901 ataacgctac tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg
     6961 ttaaaaatgg tgggtattcg atttttctct cgtttttgtc aattcgattg tccacagtta
     7021 tggtcacgcc cgtcaccaac ggcttaccgg atcgtttcac agtaagccct acgatgaaat
     7081 gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt
     7141 aattaacccg aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat
     7201 attaacgtat tttatagcac tgccgatatc acgctcaaaa aacaaccact gctattttta
     7261 gcaaagctta tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac
     7321 atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact
     7381 ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg cgatgggaca
     7441 agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga
     7501 aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta
     7561 atataaaacc ggctgagagc gtgcctttca gccggttatt gacaaaatca tcgctatctc
     7621 tgctgatgtc ttcccttccg ccgtccattt cagaatttct ttttcgcgct tgctgaaatt
     7681 catctctggc gtcatcacta tttcatcatt taaacgcatc agagccatca gactttcgcg
     7741 caccagtaac tgcattttta attgcagttc atcactaaga atgggtattt cgcgcgtgct
     7801 gcaacgggaa aaggacaaaa agcccagcgc ccgatttggc agcattaaat actgagtgac
     7861 accgcggcgt aaaccatgtg cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa
     7921 gtcatcattc cacattaaat ggccctgact aaagttttca gggttcagca caggatcaat
     7981 tgcgagaaaa ttttttgcct gataataact aacccacgac tcagggtaat tggtgtagaa
     8041 agccacttta ggtcgcgtga atggtaccgg gtggcgaaca cataacgaat agtaatcgta
     8101 ctccagctgc tgagcctgaa gctctatttc atggtagacc tcttctgcgg cctccatcct
     8161 ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa
     8221 ccgcaacgcc cctgagagtg agtatcattt ataatgataa ttcaaactat cacataaata
     8281 agatttatat ataacttata ttattcaggc aatgaattac ttttgcaagc catcgcattc
     8341 tcttatgtta ttaatgagtt atgct
//
LOCUS       DADKXM010000153         8065 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.155, whole genome shotgun sequence.
ACCESSION   DADKXM010000153 DADKXM010000000
VERSION     DADKXM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.155"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(104..895)
                     /gene="aadA1"
                     /locus_tag="JQR05_003459"
     CDS             complement(104..895)
                     /gene="aadA1"
                     /locus_tag="JQR05_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY5325512.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1044..2057
                     /gene="intI1"
                     /locus_tag="JQR05_003460"
     CDS             1044..2057
                     /gene="intI1"
                     /locus_tag="JQR05_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY5325513.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2260..2610
                     /locus_tag="JQR05_003461"
     CDS             2260..2610
                     /locus_tag="JQR05_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY5325514.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2736..3296
                     /locus_tag="JQR05_003462"
     CDS             2736..3296
                     /locus_tag="JQR05_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY5325515.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3299..6265
                     /locus_tag="JQR05_003463"
     CDS             3299..6265
                     /locus_tag="JQR05_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY5325516.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6332..6709
                     /locus_tag="JQR05_003464"
     CDS             6332..6709
                     /locus_tag="JQR05_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5325517.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(6910..7569)
                     /locus_tag="JQR05_003465"
     CDS             complement(6910..7569)
                     /locus_tag="JQR05_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY5325518.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            7848..>8065
                     /locus_tag="JQR05_003466"
     CDS             7848..>8065
                     /locus_tag="JQR05_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5325519.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARI"
BASE COUNT         1750 a         2387 c         2227 g         1701 t
ORIGIN      
        1 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa
       61 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct
      121 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
      181 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
      241 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
      301 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
      361 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
      421 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
      481 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
      541 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
      601 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
      661 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
      721 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
      781 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
      841 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt
      901 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
      961 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     1021 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     1081 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     1141 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     1201 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     1261 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     1321 tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct
     1381 tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc
     1441 atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac
     1501 tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct
     1561 ccaaggatcg ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagctgt
     1621 cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc
     1681 ccgacgccct tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt
     1741 ttgcgcagca cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc catcacatgt
     1801 atgaccagac ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc atcacgaagc
     1861 ccgccacacc gcacaccctc cgccactcgt tcgcgacggc cttgctccgc agcggttacg
     1921 acattcgaac cgtgcaggat ctgctcggcc attccgacgt ctctacgacg atgatttaca
     1981 cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc
     2041 tcactagtga gaggtagggc agcgcaagtc aatcctggcg gattcactac ccctgcgcga
     2101 aggccatcgg tgccgcatcg aacggccggt tgcggaaagt cctccctgcg tccgctgatg
     2161 gccggcagca gcccgtcgtt gcctgatgga tccaacccct ccgctgctat agtgcagtcg
     2221 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa tggaggtggt agccgagggt
     2281 gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg gttgcgacac ggtgcagggt
     2341 ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg gcttcgtcaa ccaatggagg
     2401 aacaccacca tgaacgccaa tgaaccgagc accagttgct gcgtgtgctg caaggaaatc
     2461 ccgctcgatg ccgccttcac gccggaaggg gccgagtacg tggagcattt ctgcgggctg
     2521 gagtgctatc agcgcttcca ggcgcgggcc agcactgcga ccgaaaccag cgtcaaaccg
     2581 gacgcttgtg attcgccgcc gtcaggttga ggcataccct aacctgatgt cagatgccat
     2641 gtgtaaattg cgtcaggata ggattgaatt ttgaatttat tgacatatct cgttgaaggt
     2701 catagagtct tccctgacat tttgcaggga attccatgac tggacagcgc attgggtata
     2761 tcagggtcag caccttcgac cagaacccgg aacggcaact ggaaggcgtc aaggttgatc
     2821 gcgcttttag cgacaaggca tccggcaagg atgtcaagcg tccgcaactg gaagcgctga
     2881 taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag catggatcgc ctggcgcgca
     2941 atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca acgcggcgtg catatcgaat
     3001 tcgtcaagga acacctcagt tttactggcg aagactctcc gatggcgaac ctgatgctct
     3061 cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat ccgcgagcgt cagcgcgagg
     3121 gtattgcgct cgccaagcaa cgcggggctt accgtggcag gaagaaatcc ctgtcgtctg
     3181 agcgtattgc cgaactgcgc caacgtgtcg aggctggcga gcaaaagacc aagcttgctc
     3241 gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt gagaacggat cagtaaatat
     3301 gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc ctgctggcgt tgccggactc
     3361 caaggacgac ctgatccgac attacacatt caacgatacc gacctctcga tcatccgaca
     3421 gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag ctctgttacc tgcgctttcc
     3481 cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc ttgttgaagc tggtcgccga
     3541 ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc cagcgggagc agacccggcg
     3601 cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg cccttcacca tgagccatta
     3661 ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa accgacaaag gcatcgtgct
     3721 ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc attctgcccg ccctcaacgc
     3781 cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac cggcgcatct acgacgcctt
     3841 ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac gatctgctca agcgccggga
     3901 caacggcaag acgacctggt tggcttggtt gcgccagtct ccggccaagc caaattcgcg
     3961 gcatatgctg gaacacatcg aacgcctcaa ggcatggcag gcactcgatc tgcctaccgg
     4021 catcgagcgg ctggttcacc agaaccgcct gctcaagatt gcccgcgagg gcggccagat
     4081 gacacccgcc gacctggcca aattcgagcc gcaacggcgc tacgccactc tcgtggcgct
     4141 ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc gacctgcacg accgcatcct
     4201 gggtaagctg tttaacgctg ccaagaataa gcatcagcag cagttccagg cgtcaggcaa
     4261 ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt caggcgctga tcgacgccaa
     4321 gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc atgtcctggg attcctttgc
     4381 cgagagcgtc accgaggcgc agaagctcgc gcaacccgat gacttcgatt tcctgcatcg
     4441 catcggcgag agctacgcca ccctgcgccg ctatgcaccg gaattccttg ccgtgctcaa
     4501 gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc attgaggtgc tgcgcggcat
     4561 gaacaccgac aacgcccgca agctgccagc cgatgcaccg accggcttca tcaagccgcg
     4621 ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg cgctactacg aactgtgcgc
     4681 gctgtccgag ttgaagaact ccctgcgctc gggcgacatc tgggtgcagg gttcacgcca
     4741 gttcaaggac ttcgaggact acctggtacc gcccgagaag ttcaccagcc tcaagcagtc
     4801 cagcgaattg ccgctggccg tggccaccga ctgcgaacaa tatctgcatg agcggctgac
     4861 gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg gcagccaacg acctgccgga
     4921 tgccatcatc accgagtcgg gcttgaagat cacgccgctg gatgcggcgg tgcccgacac
     4981 cgcgcaggcg ctgatagacc agacagccat ggtcctgccg cacgtcaaga tcaccgaact
     5041 gctgctcgaa gtcgatgagt ggacgggctt cacccggcac ttcacgcact tgaaatcggg
     5101 cgatctggcc aaggacaaga acctgttgtt gaccacgatc ctggccgacg cgatcaacct
     5161 gggcctgacc aagatggccg agtcctgccc cggcacgacc tacgcgaagc tcgcttggct
     5221 gcaagcctgg catacccgcg acgaaacgta ctcgacagcg ttggctgaac tggtcaacgc
     5281 tcagtttcgg catccctttg ccgggcactg gggcgatggc accacatcat catcggacgg
     5341 acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg cacatcaacc caaaatatgg
     5401 cagcagccca ggacggactt tctacaccca catctccgac caatacgcgc cattccacac
     5461 caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg ctcgacggcc tgctgtacca
     5521 cgaatccgac ctgcggatcg aggagcacta caccgacacg gcgggcttca ccgatcacgt
     5581 cttcgccctg atgcacctct tgggcttccg cttcgcgccg cgcatccgcg acctgggcga
     5641 caccaagctc tacatcccga agggcgatgc cgcctatgac gcgctcaagc cgatgatcgg
     5701 cggcacgctc aacatcaagc acgtccgcgc ccattgggac gaaatcctgc ggctggccac
     5761 ctcgatcaag cagggcacgg tgacggcctc gctgatgctc aggaaactcg gcagctaccc
     5821 gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc cgcatcgagc gcacgctgtt
     5881 catcctcgac tggctgcaaa gcgtcgagct acgccgccgc gtgcatgccg ggctgaacaa
     5941 gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc aaccgccttg gtgaaatccg
     6001 tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg tgacggcggc
     6061 catcgtgctg tggaacacgg tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg
     6121 tcatgccgtc gatgactcgc tattgcagta cctgtcgcca ctcggctggg agcacatcaa
     6181 cctgaccggt gattacctat ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc
     6241 gctacggcct ctgcaaccgg cttagcgtgc tttattttcc gttttctgag acgaccccta
     6301 ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat tatctctgta aaaagttatt
     6361 caaccggctt tccggtaccc tggtgatcag agcgcgctgc ggcaataaca ttactggcct
     6421 ggcatgttgc aatattcttt atccctcgcc ccgatacagc ggtcagctgc atattaaaga
     6481 gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt aaagcgataa tgcgctttat
     6541 agcccggctt gcgcttgaac aggaatgcct tagccttagc tggaacgctg aaaaatccaa
     6601 ccccggcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     6661 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     6721 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     6781 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     6841 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     6901 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     6961 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     7021 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     7081 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     7141 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     7201 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     7261 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     7321 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     7381 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     7441 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     7501 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     7561 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     7621 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     7681 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     7741 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     7801 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     7861 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     7921 ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg cagttcactt
     7981 acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg
     8041 gatgccgggc aaccgcccgc attat
//
LOCUS       DADKXM010000154         7968 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.157, whole genome shotgun sequence.
ACCESSION   DADKXM010000154 DADKXM010000000
VERSION     DADKXM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.157"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..285
                     /locus_tag="JQR05_003467"
     CDS             <1..285
                     /locus_tag="JQR05_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325520.1"
                     /translation="ETLEAAGHVLLLTSLSEDEYSAEQVADCYRLRWQIELAFKRLKS
                     LLHLDALRAKEPELAKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
     gene            456..890
                     /locus_tag="JQR05_003468"
                     /pseudo
     CDS             456..890
                     /locus_tag="JQR05_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            863..1288
                     /locus_tag="JQR05_003469"
     CDS             863..1288
                     /locus_tag="JQR05_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY5325521.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIVIHPECNAIYAALD"
     gene            1335..1757
                     /locus_tag="JQR05_003470"
     CDS             1335..1757
                     /locus_tag="JQR05_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY5325522.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSEFVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMEVVLQALDGEHMS"
     gene            1754..1945
                     /locus_tag="JQR05_003471"
     CDS             1754..1945
                     /locus_tag="JQR05_003471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325523.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(2315..3988)
                     /gene="ltrA"
                     /locus_tag="JQR05_003472"
     CDS             complement(2315..3988)
                     /gene="ltrA"
                     /locus_tag="JQR05_003472"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010383737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY5325524.1"
                     /translation="MNTLIKVSAAPHLGSWHTINWKRQYRTVRRLQTRIVKATQEKQW
                     RRVKALQRLLTRSFAAKALAVRRVTENSGRKTPGIDGKTWSTPAEKWNAINNLRRNGY
                     KPSPLKRIYIPKSNGKLRPLGIPTMKDRAMQALYLLALEPVAETAADRNSYGFRPHRS
                     CADAIEQVFNNLNRKTSATWVLEGDIKGCFDNISHKWLLEHIPMDKRILRSWLNAGFM
                     EKGRLFTTDAGTPQGAPCSPVLANMCLDGLETILTNRFGRKGTAKASKSKVNYVRYAD
                     DFIVTGISREILESEVKPLITAFMQERGLLLSEEKTTVTHINDGFDFLGQNVRKYKGK
                     LLIKPSKHNLKMFLNKIRATIEGNKTAKTDTLIKRLNPLIRGWTNYHQHVVAAETFRY
                     VNFQLWKKIWRWCLRRHPSRGKRWIRRKYFDESWRLHSKDADGHRWVLYKASTIPIRR
                     HIKIKSEANPYAPEWESYFEKRQALIWLKSQNGRNRIVAIWRKQEKRCPICGQSFSQE
                     TGWDIHHIVRKTQGGSDNLDNLVMLHPNCHRQLHHSSETGSQYWGLIKA"
     gene            complement(4782..4970)
                     /locus_tag="JQR05_003473"
     CDS             complement(4782..4970)
                     /locus_tag="JQR05_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325525.1"
                     /translation="MTFCGDLPFVPSGIYHHLFENLRRKGHHCHGRKQLSGDVHFPPG
                     LAETVVAVFAPGDRGLNR"
     gene            5034..5321
                     /locus_tag="JQR05_003474"
     CDS             5034..5321
                     /locus_tag="JQR05_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325526.1"
                     /translation="MSTRNIHVNTASYTLFVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            5390..6211
                     /locus_tag="JQR05_003475"
     CDS             5390..6211
                     /locus_tag="JQR05_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY5325527.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSWRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(6507..7154)
                     /locus_tag="JQR05_003476"
     CDS             complement(6507..7154)
                     /locus_tag="JQR05_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY5325528.1"
                     /translation="MIQIANQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMFAICLMFINEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTSAYYLAIAFKKWGVSWEAV
                     GAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRIPTTKKSLPEINSVQNN"
     gene            7440..7823
                     /gene="traM"
                     /locus_tag="JQR05_003477"
     CDS             7440..7823
                     /gene="traM"
                     /locus_tag="JQR05_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAY5325529.1"
                     /translation="MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYEAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANIVEDIREKVSSEMERFFPKNDDE"
BASE COUNT         1900 a         1900 c         1993 g         2175 t
ORIGIN      
        1 gaaacgctgg aagcagcggg ccatgtgcta ttgctaacat cattatcgga agatgaatat
       61 tcagcagagc aagtggctga ttgttaccgt ctgcgatggc aaattgaact ggcttttaag
      121 cggctcaaaa gtttgctgca cttggatgct ttgcgtgcaa aggaacctga actcgcgaaa
      181 gcgtggatat ttgctaatct actcgccgca tttttaattg acgacataat ccagccatcg
      241 ctggattttc cccccagaag tgccggatcc gaaaagaaga actaactcgt tgtggagaat
      301 aacaaaaatg gtcatctgga gcttacaggt ggccattcgt gggacagtat ccctgacagc
      361 ctacaaaacg caattgaaga acgcgaggca tcgtcttaac gaggcaccga ggcgtcgcgt
      421 tcttcagatg gttcaaccct taagttagcg cttatggggt aaatccccgc ctttttcctg
      481 ccggtccccg gacagagcac aaaggaagtc gccgccggct ccgctttacc cggccatgcg
      541 gtgcatggcc ttgtgggttt tcagctctgt agcctcagcg tcgtgtgcgg gctgtgccgt
      601 gcctccatct tagccgggct ggcggggatg caagggtacg cttcgccgct gcggtcaccc
      661 ggtccctcct tcccgtctgc cgtgattttc cggtcgttca cccgctcagg tttcgcggtc
      721 tcaccctcca actcccgcca gccgtgtgcg tcaggctgcg gcttcccttg cgccctgtgc
      781 atccccgcct tatcgcccgg cttttatgga ggcacggcac cgcccggtgt cgcggaatat
      841 gtgaacaacg gaggaaaaca tcatgcaata tgcgaaacct gtcactctga atgttgaaga
      901 gtgcgaccgt ctctcctttc tgccttacct gtttggcaac gattttctgt atgccgaggc
      961 gtatgtgtac gcgctggcga aaaaaatgat gccggaatat gaaggcggct tctggcactt
     1021 catccgcctg ccggacggtg gcggttacat gatgccggat ggcgaccgtt tccacatggt
     1081 gaacggtgca aactggtttg accgtaccgt gagtgctgat gccgcaggca tcattctgac
     1141 ttctcttgtg attaaccgcc agttgtggct gtaccacgac agcggggatg caggactgac
     1201 ccagctttac cggatgcgcg atgcgcagtt gtggcgtcac atcgtaattc accctgaatg
     1261 caacgcgatt tacgcggcac tggactgatt aacggacggg gcggcaatgc cgcccctgta
     1321 aaggagacga gaacatgtac tgtactgtta aagaaattat ccgcgatgta ctggatacag
     1381 acgtgccgga cagtgaattt gtttttgccg tggtgctgac ccgtggggat gtgcgtcaca
     1441 tagcccagga ctggagtctg acagacgatg agctggaaac cgtcatgcag cggctggacg
     1501 atgcttttgt atatggcgca tgtgacagag ttgtttccga tattgtcaat gaactgatgg
     1561 aggaaaagcg cgtaaaccgt cttgtaacag tccctgcggt gatgctggaa aaagtgatgg
     1621 cgctggcagg cagtgaaatg aagcgcctgt atgccgtcgg gagtgagaac gggggcgacg
     1681 gtgacgcatt cgtcagggag gaacgcgaag caatggaggt tgtgttacag gcgctggacg
     1741 gggagcacat gtcatgaata tcagcacaga aacccgcgaa attctgcgta attacagggc
     1801 cgtgattaat gcgcggcgtc gtgaaatggg gcagaaaccg ctcaccaccg cgcagattgt
     1861 tgatgaaatc tgcgattttg tggcgaatca gcaggcggtt ttcctcggtg gtcactacat
     1921 ccttcagggc agcaggcaca ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg
     1981 cgcgaaaacc cctggcaggc tgacgcctgc cggaagggaa gcgggccacg ctgacgcgtc
     2041 gcccgttacc cttcccgttt atgatgtgtc cggaatattt ttttctgatg ttatttttct
     2101 gtggttaccg tgcctgttat tgttcttctt cctcttcttc tttccgttcc tctcctctcc
     2161 tgtttatggc tttcccgggc gtccccttgg cggggctggc tgtcgtgaac ggagccagcg
     2221 aagctgtctc cgccctggcg ggtcgggtag cagcggcaca ttgctgcgcc gccgccccct
     2281 aagaacggag cgtgcgagtt tccccgcact ccgctcaagc ctttataagc ccccaatact
     2341 gagagccggt ttcactactg tggtggagtt gacggtggca gttcggatgc agcatcacta
     2401 gattatccag attgtcgctc cccccttgtg tttttcgcac aatgtgatgg atatcccaac
     2461 ccgtttcctg gctgaacgat tgtccacata ttgggcagcg tttttcctgt tttctccata
     2521 ttgctacaat cctgtttctg ccattttgag atttcagcca tatcaatgcc tgtcttttct
     2581 caaaataaga ctcccactcg ggggcgtatg gatttgcttc ggattttatt ttgatgtggc
     2641 gtcgtatagg tattgtcgat gctttataca gtacccaacg atgaccgtcg gcgtccttcg
     2701 agtgaagcct ccagctttcg tcgaagtatt tgcggcgtat ccatcgttta cctctggaag
     2761 gatgtctcct gagacaccat cgccatatct ttttccagag ttggaagttg acgtacctga
     2821 aggtttcggc tgccactaca tgttggtggt agttggtcca acctctgatc agagggttta
     2881 gccgtttgat cagtgtgtct gttttggcag ttttattacc ttcgatggtg gctctaatct
     2941 tattaaggaa cattttcagg ttatgtttag agggttttat aagtaacttc cccttgtatt
     3001 tcctgacatt ttgtccaaga aagtcaaagc cgtcatttat gtgagtaacc gtcgtttttt
     3061 cttctgacag gagcaaccct ctttcctgca tgaatgcagt tataagcggt tttacttcgc
     3121 tttccagtat ttctcgcgaa attcctgtca caatgaaatc atcggcgtaa cgcacatagt
     3181 tcactttgga tttactggcc ttggctgtgc ctttccgccc gaaacggttt gtcagaattg
     3241 tttccagtcc gtccaggcac atatttgcca gtactggtga gcagggggca ccctgaggtg
     3301 taccagcgtc agtagtgaac aatcttcctt tttccataaa tccggcattc agccagctac
     3361 ggaggatccg tttatccata gggatatgct ccagtaacca tttatgactg atattgtcga
     3421 aacacccttt aatatcccct tctagaaccc acgttgccga tgttttccgg ttcagattgt
     3481 tgaaaacctg ttcgattgca tcagcacaac ttctgtgggg acgaaagccg tagctgttac
     3541 gatccgcagc cgtttccgcg acaggttcca gagcgagcag gtagagcgct tgcatagctc
     3601 tgtctttcat cgtggggata cccagcgggc ggagtttgcc attggatttg ggaatatata
     3661 ttcgcttcag aggactcggt ttatagccgt ttctcctcag gttgttgata gcattccatt
     3721 tctctgccgg cgtgctccaa gtttttccgt cgattcccgg ggttttacgc ccggagttct
     3781 cagtaacccg tctgacagcc agtgctttgg ctgcaaacga gcgggtcagc agacgttgca
     3841 gagctttgac tctacgccat tgcttctcct gtgttgcctt cacaatccgc gtctggagcc
     3901 gtctcaccgt tcggtactgt cgcttccagt tgatggtgtg ccagctaccg aggtgcgggg
     3961 ccgcagacac cttaattagc gtgttcatct tgcctgcctc ctgtttcaga ctgctttccg
     4021 gcgtttcatg tgattaaaga ccagatggaa gtctgcacgc tttcgcgtta gggcaaattt
     4081 tgaaccccta taccatccat tacagatggc catttgcgtt ttccatcctc ctctacccgc
     4141 attttcaaca gcgttcctta cggttcgctt gccctgaggg caaaagtacg ggcttacctg
     4201 ttttcacttc agatacagat cgggttaggt tccagctctc gaccagaggc tttagtgtcc
     4261 gggtatcctc aggaagcata ggataaccag cctaacactt tttagttcag gtctatcagc
     4321 atctttgacc tgttacaggg ttatgatctt tatcactggt tcacgtgtgt taaccatacc
     4381 gatcagcctt gcctcctgtc cgcatgatgc ttgcagacga tcgtctcacc tcacggctcg
     4441 acttaccctt cctgagggag agtacgttgt catcagagct tatgaccttg ccgttaccag
     4501 caacgccatt ctgattaggc taccgctacc ggaatagcgg gatttgtcat atgtctacgc
     4561 aaatcaatat ctgaagcgac tttcagtcgc acgcttccat ccctgacgcg aaaccccggg
     4621 cttgcagcga ccgttccggt cgcgccgcct ctgtcgcgcc gtggcctgcg ctcccggcat
     4681 cccggcaaaa ggccgccggg atgtaaggcg caacgccgtt tcgcaaaccg cgcttcacgt
     4741 cgttgcggcg ggtccctgcc cgttgttgtg tccggtgcca ttcacctgtt taagcccctg
     4801 tcgccggggg caaagacagc cacaaccgtc tccgccagtc caggggggaa atgtacatcc
     4861 ccggacagtt gtttccggcc gtggcagtga tgccccttgc ggcgaaggtt ctcaaaaagg
     4921 tgatggtaaa taccggaggg aacaaaaggc aggtccccgc aaaacgtcaa aaccgcggct
     4981 cccgaaactc aaccgatgat gggggctgaa aaccagaaag aggagaaagc aggatgagta
     5041 cccgaaatat ccacgttaac accgcgtcgt acacgctttt tgttgccggg aagaaaaaaa
     5101 acaccgggga agagtgggac gtcctggaat tcagcagcct gactgagctg aaaaaatatc
     5161 gcaaaagcca cccggaaaag atggccttca gctatagcta cgcgctcagc cggggtgtgg
     5221 atacgcagtt ccgccatatc aacattgcgg aagccgatca cttcaaacag ttcctgcgtc
     5281 agattaagcg tgccggcctc gatatccggg cgatctgctg accggatgtg atgatattaa
     5341 gcggaaaaat tttttattaa atttatgctt tcataaagga gtagctgtta tgcgattagc
     5401 ttcccgtttt ggtcggtata attccatccg ccgtgaacgt cctttaacgg atgatgaatt
     5461 aatgcagttc gtgccttcgg tattttccgg tgataaacat gagtcccgga gtgaacgtta
     5521 tacgtatatt ccaacaatca atatcatcaa taagttacgt gatgaaggtt tccagccatt
     5581 ctttgcctgt cagagtcggg ttcgtgattt gggacgtcgc gaatacagta aacatatgtt
     5641 acgtcttcgc agggaagggc atattaacgg gcaggaagtt cctgaaatta tcctgcttaa
     5701 ttcacacgat ggttcatcca gttatcagat gatccccgga atttttcgtt ttgtctgcac
     5761 aaatggcctg gtgtgcggaa ataattttgg cgaaattcgc gttccacata aaggtgatat
     5821 tgtcgggcag gttatcgagg gagcgtatga agtgctcggt gtctttgata aggtcactga
     5881 aaatatggag gcgatgaaag aaattcatct taacagtgac gagcaacatt tatttggcag
     5941 agctgcactg atggtcaggt atgaagatga aaataaaacg ccagtgacgc ctgaacaaat
     6001 aattactccc cgtcgtcggg aggataaaca gaacgatctc tggacaacct ggcagcgggt
     6061 tcaggagaat atgataaaag gtggattatc gtggcgaagt gcctccggga aaaataccag
     6121 gacaagagcc attacaggta ttgatggtga tataagaatc aacaaggcgt tatgggtgat
     6181 tgccgaacag ttcagaaagt ggaagtcatg ataatataag taaagccccg aaaaattttc
     6241 ggggctttac ttatattatc attcttcggc atcgctgtgt cgtctgatat acctcaattt
     6301 catcaactgg ctttccggtt gtctgtaaat attgtgcccg cgaaaaatgg tctggcagga
     6361 ggctgcgcag ttaccgggta tatataagat gggttcccgc ttcgcggggc ttcggccagt
     6421 cagtattgtc actcatatga tatttttgtg tgtggccttc catgccgctt ttgcggcata
     6481 accggtatct gacaatgtct gtaagattaa ttgttctgca cgctgttaat ttccggaagt
     6541 gatttcttcg tggtcggaat ccggatacct ttactgcttt ttatcccggt ataaatccgg
     6601 taaacatctg atgcgtaggc aagacgtctc tggttctggc gttcggtctt cctgaatccg
     6661 gcattgtatg caccaacggc ctcccaggag acgccccatt ttttaaaggc tattgccaga
     6721 taataagcac tggtataaat gttcatgcag ggatctgttg tcagatgttc cggcttaatt
     6781 ccatagcggg ccagttcgtt aaaatgctgg gaatctacct gcatcagtcc gctgccatat
     6841 cccgttaccg gattaatacc gatggcatta acccggtaac gggattcttt ccatgatatt
     6901 gctctcagta aatcaggatc tattttgtaa tcccggcctg caagatcaaa gcaatcagtg
     6961 gcatggcaga tctcatttat aaacatcagg cagatggcga acatccattt tttcattttt
     7021 ccacctctgg tgactttatc cgtaaataat ttaacccact ccacaaaaag gctcaacagg
     7081 ttggtggttc tcaccaccaa aagcaccaca ccccacgcaa aaacaagttt ttgctgattt
     7141 gctatttgaa tcattaactt atgttttaaa taatgtattt taatttattt tacattataa
     7201 aaaggattca ttggtgaatc gcatatgatt caccaatgaa tttaacacaa ctcatagatg
     7261 aatcagtaat aataaaacaa cgagttaaag cgaattgact ctagattcaa ttcgtgatgt
     7321 gatttgctgc atgaatcgta attttcgtaa cttgcgtcac gattcgattc atcgatgaat
     7381 tgtcattttt ttatatgtga aataaatttc atgcgataag aaaataaaga ggtatacata
     7441 tggctaaagt gcaggcttat gtcagtgacg aaattgtcta taaaataaat gaaattgtgg
     7501 aaagaagacg tgcagaagga gctaaaagta cagatgtaag tttctcaagc atatccacta
     7561 tgcttctcga attagggctt cgtgtatatg aggctcagat ggagcgtaaa gagtcagcgt
     7621 tcaatcaggc tgagtttaat aaggtacttc ttgaatgtgc ggtaaaaaca cagtcaacag
     7681 ttgctaagat tttaggtatt gagtcactaa gccctcatgt ttccgggaat ccaaagtttg
     7741 aatatgcgaa tatagtcgaa gatatcaggg agaaggtgtc atctgagatg gagaggttct
     7801 ttccaaaaaa tgatgatgaa taaaattaaa ttaaaaattg attcccatca aataacagaa
     7861 tatagaactg ttctaatttg atgacaatta accacataaa gagtatattc atcatgtggt
     7921 taatcccaca ttaaaatttg aaactgaaaa tcgccgatgc agggagtt
//
LOCUS       DADKXM010000155         7948 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.158, whole genome shotgun sequence.
ACCESSION   DADKXM010000155 DADKXM010000000
VERSION     DADKXM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.158"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1103)
                     /locus_tag="JQR05_003478"
     CDS             complement(<1..1103)
                     /locus_tag="JQR05_003478"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5325530.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
     gene            complement(1465..4260)
                     /locus_tag="JQR05_003479"
     CDS             complement(1465..4260)
                     /locus_tag="JQR05_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY5325531.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            complement(4305..5735)
                     /locus_tag="JQR05_003480"
                     /pseudo
     CDS             complement(4305..5735)
                     /locus_tag="JQR05_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(5787..6491)
                     /locus_tag="JQR05_003481"
     CDS             complement(5787..6491)
                     /locus_tag="JQR05_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY5325532.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEIRLVSRVFEM"
     gene            complement(6555..7505)
                     /locus_tag="JQR05_003482"
                     /pseudo
     CDS             complement(6555..7505)
                     /locus_tag="JQR05_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(7543..>7948)
                     /locus_tag="JQR05_003483"
     CDS             complement(7543..>7948)
                     /locus_tag="JQR05_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5325533.1"
                     /translation="ADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLG
                     KLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEVIRLLRLV
                     REKLPTSGVIMVTHQPGIWNLADDICDISTVL"
BASE COUNT         1984 a         1881 c         1854 g         2229 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtca atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc aaaggactcg tgtttaaata acaaaatcct
     1201 aatgttattt atcgtgagat attgttggaa tgatattttt tcattaaaaa acaatgcaat
     1261 agagaattct tgggtggtgg caaggtgttt tatgttgtta tttttatgcg ctttctgtgc
     1321 ctcctgaatt atcacgtaaa aatcagacat taaaatatca ctattagtac ttggttatta
     1381 ttttgaacgc atttataaaa ttattacata aaaatagcga atattgctaa aatccccgcc
     1441 aacgatgtgt tgacggggct gttattattt tggcaataat actccggtat aagtatttac
     1501 cggatgagaa agatattgtt taacggcagt gttaacattc tctaccgtca tttgtttcaa
     1561 caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac tatttataat
     1621 agtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct gaacgttttg
     1681 ctggtattcg ttcagttctt gctcactgat ccccttagtc aaacgcttaa ccatcacttc
     1741 attcgctaac gttaacagtt catcatgtcg ttctggttga caagtaaaag ccagcaaatg
     1801 actgatatct ttagcctgag gatcaaccga gaggcgagaa gaaacgctgt atgccccaga
     1861 tgcctgttca cgaatattaa cgcgtagatc ttttgccagt gcgacgttaa aagcatcgag
     1921 cgccatacgc gtcgccagat taacaggtgt ccgggaatca taacgcttcc actgtgaaac
     1981 ctgtgccaca ggttcatttt gttcttttac agtaaccgat gcgttgtccg tcgcgcgagt
     2041 taatggttta cctgcggcta atggcgaatc agagtgtttg attgagccta agtaacgcgt
     2101 aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg tgatatccgc
     2161 tggagatgaa aacaactggc gatcggcagc cagcgcatct gcggcagtaa actgtgcaat
     2221 ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat acatcagctg
     2281 ggcgaatttc tccgctggac gctggtcgag cgttttcaac gcctgaattt gagcattttg
     2341 tagcgatgcc caaatattgt cgttaatcgt gctgtgggtg attcgctggt taatcaactg
     2401 gaaaccaggt tcagggttat tagtccgcgc gctaacggag agcaacgtat tcatgccact
     2461 gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc tggaagagga
     2521 gagttcgcca acgccgcttc cgctaactgc tttatttgcc agcgcgataa gtgatttttg
     2581 ctgcgcaggg aaacttaaat cgcctttatt cgatacggca ataatttgta gcttttgctc
     2641 ttcacccgcg gattttgcca gaataacctt ggcaccattg gaaagtgtta atgatgtcag
     2701 attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt ctactgttaa
     2761 cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt actctttttc
     2821 cagcgccagg atagccgcag gagacaatgc ttttttggcg gcaagctcat tgtttaccat
     2881 ttgctcccaa aatgcgtcct ggttctttct taactgctgc catttttccg ccagactttg
     2941 cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg gcgacaagaa
     3001 cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta aatcacgttc
     3061 agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga catcatcgag
     3121 ttcttcagca gaaaaaccat gctgatcaat ggttgccaac tctgccatta atgcattcgc
     3181 agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact gataatcggg
     3241 tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc ccgactgtat
     3301 gcgttcctgc agacgttgat tgaacagctg aactaacatg ctccattcag cttgttcgac
     3361 aaagctttgc tcatcgttca cttgtaccat tggcaggcga taatagagtg cgatgccgtt
     3421 cacccggttt tctttatcat taatgatatt aaagcgcagg tggttttcgg cttttgtcgg
     3481 ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattat cctttatcag
     3541 cgccagcgct tctttactgt cgatatcgcc gaccacgata aaggtcatat tatttggttg
     3601 ataccagcgt tgataaaatt ggcgcaattg tgccggtgtg accgtggcga cagtatccat
     3661 caggccgata ggttcacggt ctaaattacg ggtattagcc agcaggaaag ggcggcgcgc
     3721 ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa ttacgccacg
     3781 ttcagcgtct acttcgagtt tttcaaaggt tgcggcatta ctccattcac tgaagattgc
     3841 catcacttgt tgcagatttt gtttctgcgt agtcggcaaa ctcacctgat acaccgtttc
     3901 gtcatagctg gtataggcat taacatcgcg accaaaacgc aggcccattg actcaaatgt
     3961 ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat gttctacaaa
     4021 atgagccacg ccgcgctcat tgtcttcttc ctgcaatgaa ccggtatgaa tttgcagcca
     4081 taaatttacc tgatcctttg gatgagcatg cggataaatc atatatcgca agccattgtc
     4141 cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca gccgcccggg
     4201 gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa ttatctccat
     4261 gcgaaaaccg ggcgaattta cccggttaag taaaatccga actattaaaa tttcatgctg
     4321 acatcaagcc agaaagtacg gccactggcg taggtcgcca caccggtgtt cgttgtatca
     4381 acagcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag gttttgttgt
     4441 ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct atccagtttt
     4501 tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt tgtcttacca
     4561 aggataatgc gagctttacg cgcttcttgc caggccagcg tattcgccca cactaacccg
     4621 ctcggttgat gcgtgaaatc catgtttaag gagatcttta atgggttatt aaaatctgcc
     4681 actggaacgc tatcgtaaga gaccagatta ccgttataaa ccacctggtt atcaccggta
     4741 ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat atagctaaag
     4801 acaatttgcg ggttaatatc tacctggcgg atatgcaggg gttcggccag ttcaaaactg
     4861 aggttaaacg aatgcgtttt ggttttgcca tcgttgttat attcagtaat ggtggtttta
     4921 gtcgcgctgt cggtacgact gcttttgctg atttgatcat gcgcttcacg gtaaacatag
     4981 tttgcgcgtg caataacgtt cttatcgatt ttttgctgca atcccattgc cagttcatcg
     5041 ttataaggcg ttttcaaatt ctgataacgc gtcagggttt tattacctga taccgattcc
     5101 gtccagctat tgcggatatc acgtaatccc atattaagaa tattcccgcc atagtaacgg
     5161 ttataaccgg cggtaatcat tgaggtttga tcagcaaaaa tatcccattc cgtcataaag
     5221 cgcggggaga tattgtggtt tgacagatag ttgtcatagt cataacgcac accgggcatt
     5281 aacgacacat tccgccagct aatgtgatcc gccatataca gcgtgtagtt gtcaattccc
     5341 aggctgcctt tacctttatg gtaaatggta tggttagttt tctttcctgc agcattaatc
     5401 acataggatt cagactggtt atggcgttca gtccatgcat cggaatagat gtattccgcg
     5461 ccgaagtagg gttgatgcga aacatcaccc acggcgaatt tttgccagtc caggcgtgtt
     5521 ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg ggtacaacgc
     5581 cctgtaatat caccatatgt acatgaaagt tcggtgtacc agatgtcatg atcgtgacgg
     5641 gtataatcac taatatgatc ccaaccaacg gtggtacgta gtttggccca tgcgagttgc
     5701 gtatccatat cccatgccag accataggat ttatggcact gttgcaaagt tagcgatgag
     5761 gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac caggcgtatt
     5821 tcgcccaggg gatcaccata ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc
     5881 acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg
     5941 ccgattatcc gtttcagttt gccatgatcg cattcaatca cgttgttccg gtacttaatc
     6001 tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga
     6061 ccataggcgg gcgctttatc cgtgttgatg aatcgcggga tctgccactt cttcacgttg
     6121 ttgaggattt tacccagaaa ccggtatgca gctttgctgt tacgacggga ggagagataa
     6181 aaatcgacag tgcggccccg gctgtcgacg gcccggtaca gatacgccca gcggccattg
     6241 accttcacgt aggtttcatc catgtgccac gggcaaagat cggaagggtt acgccagtac
     6301 cagcgcagcc gtttttccat ttcaggcgca taacgctgaa cccagcggta aatcgtggag
     6361 tgatcgacat tcactccgcg ttcagccagc atctcctgca gctcacggta actgatgccg
     6421 tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg
     6481 aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     6541 tttgcaacag tgccggattt attacccatt tcgcgatcag actgcgggaa ggtgctggta
     6601 ttataatcac ggctggagcc ggtatatttt aaggttaaat cgtgggtaaa gcggtcgctg
     6661 gcaaaccagg taaatttgct caatgcagta tcgataacgt ttttatactg tgcccgaccg
     6721 gcgacaatgc cgtcattcga aacataatcc gcgcgggtga tatcagactg gcggcgcgat
     6781 aaaccggcgg taacgccaaa gttatcagcc agctcctgat taaacgacaa ggtataaaag
     6841 ttctttttaa aatctggaga gaaataggta cttcctgaag aaccttgatt aaatgcgctc
     6901 ttgttattct catcgatatg cgatgttaac cagtccaaac gcgtagtgcg ataacccaat
     6961 ttcaccttgc tatcatcagc gttgaagcgt ttgatctttg catcaattac cccgccattg
     7021 aagcgaccaa attcaaccgg cacaaaactg tcataaagcg tcacattgtc cagtaagctg
     7081 acatcaagat aatacccctg tgacatcccg ctaatattgg ttgcactact ggcatcggaa
     7141 tcattcgctg ggttcaggtt attagttgca ctaataccgt caatcaaata ggcattctgg
     7201 tagggcgacg caccgtgaat agagattttc tcagggcgaa tatctccctg gttcaacgag
     7261 gtactttgcg ttgaatccat gcgtaccgca gggttggttc tcagcagatc gctgatattg
     7321 ccattcccgg tcggtaaacg ctcgatgctt tcactggtgt aatgcgtgtt gccgtttacg
     7381 ggtacaggga caggagcata gaccgtcatc tcttcaaaaa aatgcatgta catgtttttg
     7441 tcatcgacgg cctgcgccac atcagcgcca cataaaatga cgccaggaat aagaactcgc
     7501 ttcatttatt tatatctccg tacagcaggg cttttattgt tgttataaaa ccgtgctaat
     7561 gtcacaaata tcatcagcca ggttccagat accgggttga tgtgtaacca taatgacgcc
     7621 gcttgtgggt agtttttcac gcactaaacg cagtaagcgt ataacctctt gttcctcaag
     7681 atgagaggta gtttcgtcaa gaaatatcca tttcggacgt cgtaaaatta atcgtgccag
     7741 ggcgatacgt tgtttttcgc cgctggaaag aatatctccc cagcgatcgt ggtcatgaat
     7801 acgcgcagcc aatttcccca gaccaacctg atgcagtact tcgctcaacg atttatcgtc
     7861 tacgggcagg ggaagtgctt tacaaataat ctctttcagt aagccgcttt tgattaacgg
     7921 tgtttgtgac acataccaac tgtcagca
//
LOCUS       DADKXM010000156         7857 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.159, whole genome shotgun sequence.
ACCESSION   DADKXM010000156 DADKXM010000000
VERSION     DADKXM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.159"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            185..946
                     /locus_tag="JQR05_003484"
     CDS             185..946
                     /locus_tag="JQR05_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5325534.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1083..2420
                     /gene="adiC"
                     /locus_tag="JQR05_003485"
     CDS             1083..2420
                     /gene="adiC"
                     /locus_tag="JQR05_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY5325535.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDRLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2524..4167
                     /gene="eptA"
                     /locus_tag="JQR05_003486"
     CDS             2524..4167
                     /gene="eptA"
                     /locus_tag="JQR05_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY5325536.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4164..4832
                     /gene="basR"
                     /locus_tag="JQR05_003487"
     CDS             4164..4832
                     /gene="basR"
                     /locus_tag="JQR05_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY5325537.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4833..5933
                     /gene="pmrB"
                     /locus_tag="JQR05_003488"
     CDS             4833..5933
                     /gene="pmrB"
                     /locus_tag="JQR05_003488"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY5325538.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5909..5998)
                     /gene="pmrR"
                     /locus_tag="JQR05_003489"
     CDS             complement(5909..5998)
                     /gene="pmrR"
                     /locus_tag="JQR05_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY5325539.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6110..7612)
                     /gene="proP"
                     /locus_tag="JQR05_003490"
     CDS             complement(6110..7612)
                     /gene="proP"
                     /locus_tag="JQR05_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY5325540.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
BASE COUNT         2001 a         1993 c         1913 g         1950 t
ORIGIN      
        1 ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta taaaaatttg
       61 taatattagt aaaactcgtt atttttatgc atgtttatat tcatcataca attatataac
      121 catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac tcaggagtac
      181 atatatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa aagatgtctg
      241 gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg cgttattaaa
      301 ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg ctcatattag
      361 tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc aaataccagt
      421 atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag acgtctttcg
      481 cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg aacgaacacg
      541 cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc tttcattaat
      601 gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg aaagcgatat
      661 tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc caagtctgtt
      721 aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca cctgccgcat
      781 gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg tatcacagtc
      841 ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct acggtatgac
      901 gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt taaccttaac
      961 gaagagctat attaataacg gcatcagcga taacccggtc gataataatt caactatcga
     1021 atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa
     1081 ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt
     1141 cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc tccactggcg
     1201 ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc tcgatggtat
     1261 acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac gcccgccgct
     1321 gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc tgctggatcg
     1381 gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc ccgattctga
     1441 aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc gtcctgctga
     1501 acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg ctggcgctga
     1561 tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa acctatatgg
     1621 cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc cttaacgtta
     1681 cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc
     1741 cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc gtttgctatg
     1801 tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc gtttctgcct
     1861 cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc attgtttcct
     1921 tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa
     1981 cggcgaaagc cgctgccgat gacagactgt tcccaccgat ttttgcccgt gtaaataaag
     2041 cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc ttccagctca
     2101 gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc tcggtcatct
     2161 ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc cacggtcact
     2221 ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc tactgcattt
     2281 gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc ctgatggtca
     2341 tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat cccttagatg
     2401 caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca accttaagaa
     2461 cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga tggaaacacc
     2521 gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg gctattgttg
     2581 gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg
     2641 ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt cgtcgctttc
     2701 agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg accactggcc
     2761 tgcctgttta ttctggttgg cgcggctgca caatatttca taatgactta cggcatcgtc
     2821 atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag ttatgcgctg
     2881 atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc gctgattgcc
     2941 tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt ccgtggagcc
     3001 aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta taaagactac
     3061 gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc taacagcatt
     3121 gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct ggtgcgaatt
     3181 ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa tttgaccatc
     3241 ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta cccgcgtgaa
     3301 actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc atcttgcggc
     3361 acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga gcactacaaa
     3421 gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac
     3481 gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag
     3541 aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta tgacgaagtg
     3601 ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt gattgtctta
     3661 cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca gttcaggaaa
     3721 tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca actggtgaac
     3781 acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat taatctgctt
     3841 aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca cggtgaatcg
     3901 ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc ggatagccaa
     3961 aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta tcaggttgac
     4021 cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa tttattctcc
     4081 acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga tgatattctg
     4141 caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat acgctgttat
     4201 tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc gtgacaaccg
     4261 cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta ctggatttag
     4321 ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc
     4381 tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc gggctggatg
     4441 tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac gcccgtatcc
     4501 gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt ggcaatctga
     4561 cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt ctgacgccca
     4621 aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta catcgggaaa
     4681 ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc ctggaagtgc
     4741 atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg
     4801 gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt ctgcgccgac
     4861 caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg gtgtttgagc
     4921 tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg tttgagcagg
     4981 cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc gaggcggtcg
     5041 ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc tgctatcagg
     5101 cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa gcgcgcaccg
     5161 ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa gcggtggttt
     5221 cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa aggttgttta
     5281 ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg catctggaac
     5341 tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt gatcagatga
     5401 tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt tcttccggta
     5461 attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac gaactcagta
     5521 ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc gacatcaccg
     5581 ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cttggtagaa aacgcccatc
     5641 gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc ggagcggtca
     5701 tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag ttgagtaaag
     5761 cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt attgtcagcc
     5821 gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag acttccggca
     5881 cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata taaagaaagc
     5941 tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg tttttcattg
     6001 cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg aaccttaaga
     6061 gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct tattcatcaa
     6121 ttcgcggatg ttgctgcacc aagcgggtac gtttcgcctg caaatcggca atctcgtggt
     6181 caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt tccttcgctt
     6241 cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca
     6301 tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag gcaggcatca
     6361 tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga ccggcaacca
     6421 gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc gggaacatcg
     6481 ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc agcatcagta
     6541 acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc gggatcgcca
     6601 acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa cggtcactca
     6661 gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata ataatcagca
     6721 ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata taggtcagca
     6781 acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac aggctgcgcc
     6841 agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt
     6901 cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc agcgcatggc
     6961 gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata cgccagcccc
     7021 agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg cccgcaccca
     7081 gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca cgtttacggt
     7141 caggggagta ttccgcaaca aatatcgacg ccccagtata ttcaccgccg accgagaaac
     7201 cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc gtgtcgtagg
     7261 acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg atagcgagga
     7321 tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca agcggtcgaa
     7381 tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc
     7441 ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca aaatcaaacc
     7501 attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta ccgtcatcaa
     7561 taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc atagctttcc
     7621 tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg
     7681 gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct tacaccctcc
     7741 ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa tgaccttttt
     7801 accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt aggactc
//
LOCUS       DADKXM010000157         7782 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.160, whole genome shotgun sequence.
ACCESSION   DADKXM010000157 DADKXM010000000
VERSION     DADKXM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.160"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            108..641
                     /gene="ymdB"
                     /locus_tag="JQR05_003491"
     CDS             108..641
                     /gene="ymdB"
                     /locus_tag="JQR05_003491"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY5325541.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRYALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            583..2064
                     /locus_tag="JQR05_003492"
     CDS             583..2064
                     /locus_tag="JQR05_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY5325542.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2072..3229)
                     /gene="mdoC"
                     /locus_tag="JQR05_003493"
     CDS             complement(2072..3229)
                     /gene="mdoC"
                     /locus_tag="JQR05_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY5325543.1"
                     /translation="MNPVPAQREYFLDSIRTWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3623..5158
                     /gene="mdoG"
                     /locus_tag="JQR05_003494"
     CDS             3623..5158
                     /gene="mdoG"
                     /locus_tag="JQR05_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY5325544.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KQHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5151..7694
                     /gene="mdoH"
                     /locus_tag="JQR05_003495"
     CDS             5151..7694
                     /gene="mdoH"
                     /locus_tag="JQR05_003495"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY5325545.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMLEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1929 a         1854 c         2055 g         1944 t
ORIGIN      
        1 cgaatataac taagtttctg aacaactcac gttgctgagc agaaaaatgc gatttaacca
       61 aaaagcctgc tgtacactta agaaacaaga aggtgtaagg aggcgttatg aaaacgcgta
      121 ttcatgttgt gcagggtgat attaccaaac tggccgttga tgtgattgtg aatgcggcta
      181 atccgtcatt aatgggaggc ggcggcgtcg atggggccat tcatcgcgca gcgggtccgg
      241 ccctgctgga tgcctgttta aaagtcaggc aacagcaggg cgattgccct acggggcatg
      301 ccgttattac tcttgcaggc gatcttcccg ctaaagccgt agtacacacc gtcgggccag
      361 tctggcgtgg tggtgaacaa aacgaagacc agcttttgca ggatgcctat ctgaatagcc
      421 tacgactggt ggcggcaaac agctatacgt cagtggcttt tcctgcaatc agtactgggg
      481 tttatggtta ccctcgtgcg gcagcggctg aaatcgcagt taaaaccgtt tcagaattta
      541 ttacccgtta cgctttaccc gaacaggtat actttgtctg ttatgatgaa gaaaacgccc
      601 acctctacga aagactcctt acccaacaag gagatgaatg atttgccccg gctggcgagc
      661 gcggtgctgc cactgtgttc gcaacatccc ggtcagtgtg gtcttttccc tctggagaaa
      721 agtctggatg cgtttgccgc ccggtatcgt ctggccgaaa tggcagagca tacgctggat
      781 gttcagtatt acatctggca ggacgatatg tcggggcggt tactgttttc cgccctgtta
      841 gccgcagcaa agcgtggcgt tcgcgtccgt ttgttgctgg acgacaacaa tacgcccgga
      901 cttgacgaca ttttacgctt gcttgacagt catccacgca ttgaagtccg gctttttaat
      961 cctttctcgt ttcgcttgct acgtccgctt ggatatatca ccgacttttc ccgtctcaat
     1021 cgccggatgc acaataaaag tttcactgtc gatggcgtgg ttaccctagt gggaggacga
     1081 aatattggtg atgcctattt tggtgcaggg gaagagccac ttttttcgga tttagatgtc
     1141 atggcaatag gccccgtggt agaggacgtt gccgatgatt tcgcccgcta ctggtattgc
     1201 aaatcggttt cacccttaca gcaggtgctg gatgtcccgg aaggtgaaat ggcggatcgc
     1261 atcgagttac ccgcctcctg gcataacgat gccatgacgc atcgttattt acgcaaaatg
     1321 gaatccagtc catttataaa tcatctggtt gatggaacgt tgccgcttat ctgggcgaag
     1381 acacgtttat taagtgatga tccggcgaaa ggggagggca aggcaaaacg gcattcactg
     1441 ttaccgcagc gtctgttcga tatcatgggc tcacccagtg aacgcatcga tattatctct
     1501 tcctattttg taccgacacg cgcaggtgtg gcgcaactct tacggatggt gagaaaaggg
     1561 gtaaagattg cgatcctaac caattctctt gccgctaacg atgttgctgt cgtccatgcc
     1621 ggatacgcgc gctggcgcaa aaaattgctc cgctatggcg tggaattata tgaactcaag
     1681 ccgacgcgtg aacaaagcag tacgttacac gatcgcggca taaccggtaa ttccggtgcc
     1741 agcctgcatg ctaaaacttt tagcatcgat ggtaaaacgg tgtttatcgg ctctttcaat
     1801 ttcgatccgc gttcaacatt gctcaatact gaaatgggct tcgtgataga gagcgaaacg
     1861 ctggcacagt taattgataa acgctttatt cagagccagt acgatgcggc ctggcagctc
     1921 cgtctggaca ggtggggacg gatcaactgg gttgatcgtc atgcaaagaa agaggttatt
     1981 ctcaaaaaag aacccgccac cagtttctgg aaacgggtta tggtcagact ggcgtcgata
     2041 ttgcccgtgg aatggttatt gtaaatgtgg cttaacgggc tggtgcttta tcgttctcac
     2101 gcttaacaac cggtttacca gagaacaaaa acttcagtaa cgggatgcgt aaatgaattt
     2161 catacagaat tatcgcaatc cctactacga atatcaggcc acagagaaaa ccaagccagt
     2221 tggaggtgat gtgcggtgta atgtatgcgc cgaaaaacag cgttaacggg tggtgaacca
     2281 gatagataaa cagcgatgcg ttaacaaaat aagtcacccg cgctgactgg aagttaagca
     2341 aacggtggcc aaaggagaag accacattca ccatccacag gccgaggacc atggtgatca
     2401 ccgactcggt ttcgtacatc caggcatcgc cactgccata gcgctggttg agtaaataag
     2461 cgacaaacgc caatgctgca gcgagggtac agccacgaga cggcgtggta aacaaggctt
     2521 taagatgagg gaaaatgaaa gccagtgcgc cgaggataaa gaacggtaaa taaaatagcg
     2581 tttgcatgac aataaaattg aacatgccat tactcagaat gggtggatac acaataaaaa
     2641 tcgttcttct tattaccgca tagccgatgc cgaggcataa aaaaatcacc gatagtttta
     2701 ccatcgagaa ttttttattc gttttatcag aattttctaa attatttctg atgcgcttaa
     2761 atatccatac acacagcgtc gtcatgacta ctaacaccag taaaaaccac aggtgtgata
     2821 ttaattccca ggccaacgta ttatatttgt catacaatga tagcctaggc caactttccg
     2881 ctttcccttt gacatattgc agcataataa attgcggtaa tgtcagtagg gggatggctg
     2941 ttaacatcgg gatacctaca cgttcgacac gtactttcca ccattttttc aagggatagc
     3001 gtaaaaaaag catgtaggaa aagtatccgg atataacgaa aaatacctgc atgcggaacg
     3061 agtggatgaa gtcattaaaa agggtcagcc acaatgacgg ttcggcgcta ttcacatgcc
     3121 atgtatggct cgaatagatt aaagaaatat gaaaagggat ccctaacaac atcagccagg
     3181 tgcggatgga gtcgaggaaa tattcacgtt gcgcgggtac tggattcata tatggttaac
     3241 taatctcgga tttttcgtct tatccctgtc gggttatgcc tttaggcttg ttgccatagc
     3301 gacaccgacc tgaccgcgcc aggcgcaggc ttcaaggttt ttatgcatag catcatcgct
     3361 accactaacc agaatggaag cgtctgtaag acggttgata aataaatttg ctggcaaacc
     3421 ctacacgaag tcgatgcttc tgtctttagg agaagcacgg aaagtgaaaa cggttgcaat
     3481 caggtgctta atccatgagc cagcgtgctg aacgataccg ggattcagtt gtcggaatgg
     3541 ctggttatcc attaaaatag atcggatcga tataagcaca caaaggggga agtgcttact
     3601 aattatgaaa cataaactac aaatgatgaa aatgcgttgg ttgagtgctg cagtaatgtt
     3661 aaccctgtat acatcttcaa gctgggcttt cagtattgat gatgtcgcaa agcaagctca
     3721 atccttagcc gggaaaggct atgaggcgcc taaaagcaac ttgccctccg ttttccgcga
     3781 catgaaatac gcggactatc agcagatcca gtttaatcat gacaaagcgt actggaacaa
     3841 tctgaagacc ccattcaaac tcgagttcta ccatcagggt atgtacttcg ataccccggt
     3901 caaaataaat gaagtgactg ccaccgcagt caaacgaatc aaatacagcc cggattattt
     3961 cactttcggc gatgttcagc atgacaaaga cacggtaaaa gaccttggtt ttgccggttt
     4021 taaagtgctt tacccgatca acagcaaaga taaaaacgat gaaatcgtca gcatgctcgg
     4081 ggccagctat ttccgcgtga ttggtgcagg tcaggtttat ggcctttctg cccgcggcct
     4141 agcaattgat accgctttgc catcgggtga agaatttcca cgcttcaaag agttctggat
     4201 cgagcgtcca aaaccgactg ataaacgttt aaccatttat gcattgcttg actcgccgcg
     4261 tgcgacaggt gcttacaaat tcgtggttat gccagggcgt gacacggttg tggatgtgca
     4321 gtcgaaaatc tatctgcgcg ataaagtcgg caaactgggg gttgcaccgt taaccagtat
     4381 gttcctgttt gggccgaacc aaccgtcgcc tgcaaataac tatcgtccgg agttgcacga
     4441 ctctaacggt ctgtctatcc atgctggtaa tggcgaatgg atctggcgtc cgttgaataa
     4501 cccgaaacat ttagcggtca gcagcttctc catggaaaac ccgcaaggct ttggtctgtt
     4561 gcagcgaggt cgtgatttct cccgctttga agatctcgat gatcgttacg atctccgtcc
     4621 aagcgcatgg gtgactccga aaggggagtg gggcaaaggc agcgttgagc tggtggaaat
     4681 tccaaccaac gatgaaacca acgataacat cgtcgcttac tggacgccgg atcagctgcc
     4741 ggagccgggt aaagagatga actttaaata caccatcacc ttcagccgtg atgaagacaa
     4801 acagcatgcg ccagataacg catgggtgca acaaacgcgt cgttcaacgg gggatgtgaa
     4861 gcagtcgaac ctgattcgcc agcctgacgg tactatcgcc tttgtggtcg attttactgg
     4921 cgcagagatg aaaaaactgc cagaggatac cccggtcaca gcgcaaacca gcattggtga
     4981 taatggtgag atagttgaaa gcacggtgcg ctataacccg gttaccaaag gctggcgtct
     5041 ggtgatgcgt gtgaaagtga aagatgccaa gaaaaccact gaaatgcgtg ctgcgctggt
     5101 gaatgccgat cagacgttga gtgaaacctg gagctaccag ttacctgcca atgaataaga
     5161 caactgagta cattgacgca atgcccatcg ccgcaagcga gaaagcagca ttgccgaaga
     5221 ctgatatccg cgccgttcat caggcgctgg atgccgaaca ccgcacctgg gcgcgggagg
     5281 atgattcccc gcaaggctcg gtaaaggcgc gtctggaaca agcctggcca gattcacttg
     5341 ctgatggaca gttaattaaa gacgacgaag ggcgcgatca gcttaaggcg atgctagaag
     5401 caaaacgctc ctcgatgttt cccgacccgt ggcgtaccaa cccggtaggc cgtttctggg
     5461 atcgcctgcg tggacgcgat gtcacgccgc gctatctggc tcgtttgacc aaagaagagc
     5521 aggagagcga gcaaaagtgg cgtaccgttg gtaccatccg ccgttacatt ctgttgatcc
     5581 tgacgctcgc gcaaactgtt gtcgcgacct ggtatatgaa gaccattctt ccttatcagg
     5641 gttgggcgct gattaatcct atggatatgg ttggtcagga tttgtgggtt tcctttatgc
     5701 agcttctgcc ttatatgctg caaaccggta tcctgatcct ttttgcggta ctgttctgtt
     5761 gggtgtccgc cggattctgg acggcgttga tgggcttcct gcaactgctt attggtcgcg
     5821 ataaatacag tatatctgcg tcaacagttg gcgatgaacc attaaacccg gagcatcgca
     5881 cggcgttgat catgcctatc tgtaacgaag acgtgaaccg tgtttttgct ggcttgcgtg
     5941 caacgtggga atcagtaaaa gccaccggga atgccaaaca ctttgatgtc tacattctga
     6001 gtgacagtta taacccggat atctgcgtcg cagagcaaaa agcctggatg gagcttatcg
     6061 ctgaagtcgg tggcgaaggt cagattttct atcgccgccg ccgtcgccgc gtgaagcgta
     6121 aaagcggtaa tatcgatgac ttctgccgtc gctggggcag ccagtacagc tacatggtgg
     6181 tgctggatgc tgactcggta atgaccggtg attgtttatg cggcctggtg cgcttgatgg
     6241 aagccaaccc gaacgccggg atcattcagt cgtcgccgaa agcgtccggt atggatacgc
     6301 tgtatgcgcg ctgtcagcag ttcgcgaccc gcgtgtatgg gccactgttt acagccggtt
     6361 tgcacttctg gcaacttggc gagtcgcact actggggaca taacgcgatt atccgcgtga
     6421 aaccatttat cgagcactgc gcactggctc cgctgccggg cgaaggttcc tttgccggtt
     6481 caatcctgtc acatgacttc gtagaagcgg cgttgatgcg ccgtgcaggt tggggggtct
     6541 ggattgctta cgatctcccg ggttcttatg aagaattgcc gcctaacttg cttgatgagc
     6601 taaaacgtga ccgccgctgg tgccacggta acctgatgaa cttccgtctg ttcctggtta
     6661 agggtatgca cccggttcac cgtgcggtgt tcctgacggg cgtgatgtct tatctctccg
     6721 ctccgctgtg gtttatgttc ctcgcgctct ctactgcatt gcaggtagtg catgcgttga
     6781 ccgaaccgca atacttcctg caaccacggc agttgttccc ggtgtggccg cagtggcgtc
     6841 ctgagctggc gattgcactt tttgcttcga ccatggtgct gttgttcctg ccgaagttat
     6901 tgagcatttt gcttatctgg tgcaaaggaa cgaaagaata cggcggcttc tggcgcgtta
     6961 cattatcgtt gctgctggaa gtgctgtttt ccgtgctgct ggctccggta cgcatgctgt
     7021 tccatacggt cttcgttgtc agcgcgttcc ttggctggga agtggtgtgg aattcaccgc
     7081 agcgtgatga tgactccact tcatggggcg aagcgttcaa acgccacggc tcacagctgc
     7141 tgttggggtt agtgtgggct gttgggatgg cgtggctgga tctgcgtttc ctgttctggc
     7201 tggcaccgat tgtcttctcg ttgatcctgt caccgtttgt ttcggtgatt tccagccgtg
     7261 ccaccgttgg tctgcgcacc aaacgctgga aactgttcct gatcccggaa gagtattcac
     7321 cgccgcaggt gctggttgat accgatcggt tccttgagat gaatcgccaa cgctcccttg
     7381 acgatggctt tatgcacgca gtgtttaacc cgtcatttaa cgctctggca accgcaatgg
     7441 cgacagcgcg tcaccgcgcc agtaaggtgc tggaaatcgc ccgtgaccgc cacgttgaac
     7501 aggcgcttaa cgagacgcca gagaagctga atcgcgatcg tcgcctggtg ctgctaagcg
     7561 atccggtgac gatggcccgt ctgcatttcc gcgtctggaa ttcccctgaa agatattctt
     7621 catgggtgag ttattacgaa gggataaagc tcaatccact ggcattgcgt aaaccggatg
     7681 cggcttcgca ataaaaacgt agttgcctga tgcgctacgc ttatcaggcc tacatcgttc
     7741 ctgcaattta ttgattttgc aagattttgt aggtcggata ag
//
LOCUS       DADKXM010000158         7756 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.161, whole genome shotgun sequence.
ACCESSION   DADKXM010000158 DADKXM010000000
VERSION     DADKXM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.161"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..893)
                     /locus_tag="JQR05_003496"
     CDS             complement(<1..893)
                     /locus_tag="JQR05_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY5325546.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="JQR05_003497"
     CDS             953..1231
                     /locus_tag="JQR05_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325547.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JQR05_003498"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JQR05_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY5325548.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JQR05_003499"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JQR05_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY5325549.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="JQR05_003500"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="JQR05_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY5325550.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="JQR05_003501"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="JQR05_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY5325551.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="JQR05_003502"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="JQR05_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="JQR05_003503"
     CDS             complement(4911..5441)
                     /locus_tag="JQR05_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325552.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JQR05_003504"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JQR05_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY5325553.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6410..7633)
                     /locus_tag="JQR05_003505"
     CDS             complement(6410..7633)
                     /locus_tag="JQR05_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325554.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSENKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2242 a         1532 c         1622 g         2360 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaata cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggtcaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatagaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgtgtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttgatag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc accccagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgc tgttcatatt
     6841 tatgtagttt ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt
     6901 cagtgaaatt tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct
     6961 tggacatatc ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca
     7021 tcctattttc atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt
     7081 gaatgtctct tatatcagtt ggtaagtcag ctcgttgccc agcaaaattt ttattttcgg
     7141 aagaaacggt ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta
     7201 tgattggatt gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat
     7261 ccttttcgta tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg
     7321 gtgtcgaact tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg
     7381 agtagtctgc atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt
     7441 gttgcgctaa atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg
     7501 gttgttgttc ttcccaatta tcacaaggcc acttcggtgt gtctggcgtt ttaacattat
     7561 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctggggtaa
     7621 agtcaattga cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg
     7681 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
     7741 ctgatataat tacctt
//
LOCUS       DADKXM010000159         7553 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.162, whole genome shotgun sequence.
ACCESSION   DADKXM010000159 DADKXM010000000
VERSION     DADKXM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.162"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..1542)
                     /locus_tag="JQR05_003506"
                     /pseudo
     CDS             complement(1..1542)
                     /locus_tag="JQR05_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1488..1709)
                     /locus_tag="JQR05_003507"
     CDS             complement(1488..1709)
                     /locus_tag="JQR05_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325555.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLRKAEYITARRPA
                     GQKKKHDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(1754..3690)
                     /locus_tag="JQR05_003508"
                     /pseudo
     CDS             complement(1754..3690)
                     /locus_tag="JQR05_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(3754..5415)
                     /locus_tag="JQR05_003509"
     CDS             complement(3754..5415)
                     /locus_tag="JQR05_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY5325556.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYGAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5412..5975)
                     /locus_tag="JQR05_003510"
     CDS             complement(5412..5975)
                     /locus_tag="JQR05_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY5325557.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQQAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6264..6629)
                     /locus_tag="JQR05_003511"
     CDS             complement(6264..6629)
                     /locus_tag="JQR05_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY5325558.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKHHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     TTLPEVSGGSGRADDIIGL"
     gene            6671..6871
                     /locus_tag="JQR05_003512"
     CDS             6671..6871
                     /locus_tag="JQR05_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY5325559.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7003..7329)
                     /locus_tag="JQR05_003513"
     CDS             complement(7003..7329)
                     /locus_tag="JQR05_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY5325560.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT         1660 a         2374 c         1823 g         1696 t
ORIGIN      
        1 tcaagcatcg gacgcatatc atcgcccgtc acgcacttct gtaataccac agacagggac
       61 gccagttttt cttcaaacgt ttgtgcctgc gcggctattt tcccctcaaa tgtgcgctgt
      121 aaatccgcca gcactgcgga aaattcttca cccagcgcac ggataatgga cagttcacgt
      181 tcattcattt tttcagaatc cccctgaaca tcgccttcac cgcatcatgc tctgtttcag
      241 tgattgcctt attaccgtca gatgcgctgt caggcggttg cgctaaggcc gttttcccgg
      301 tcgacgcgaa cggatcttca cgggcatcac gacgggacag cgcctccaga ctgtagttct
      361 gctgctgaag atacagtgca tcacctcccg caaggggcgg caggttctca cgtttacggg
      421 cctcattggg cgtgagaagc gtatttttca ccgactcacc cagcgttttc atgcgccgtt
      481 cgctgtccat tctcagcagc gtggtgacgt caaactccgt actctcgttt tcccccgttt
      541 ccagcgcctc atccagtaac agttcaatgg actcaatcag cgtctgcagg cactgggaat
      601 aatactgctg ctccagcgcc tccacgttgt cactggaagg tggctggcca acgccaatct
      661 tgtaggccgg gacacggaac accgaacaga caatttcagc ggtcatcttc agttgttcca
      721 ccgtctgcgc atccaccggt gaaaacgtcg tgaggttgta ttttgccccg ttgctcaaaa
      781 tggccgtttt ccccgcattt tcgccggtat acccgctgtc ccagttgctc ttcagttttt
      841 tcgcattttc ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat
      901 ttctgaaaaa agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg
      961 cggcatacac cggcggcagc cccacaagcg gatgaaaaaa acagttaaac cggtcgcttc
     1021 ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg tgatgcgata
     1081 gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca gaatacgcag
     1141 ttctttgatc tgcccccggg agttacggat tttcagcacc accgtattgc cgtgacgcag
     1201 tttggcgttc agccacagtt caaaaaactg gatacgattc tgctgtgcat tgggacgacg
     1261 acagagacgg gcaatatccc cctgccgttt ttcacggcgg atcccctgtg tatccgtctg
     1321 catcaggcgc agccgcattt tggcgatatc ctgggatatc agcgaaatgc aagaaaacac
     1381 cgcatgaaag gagaggacac tttccggatc ggctttcaca ccctgctgcc aggcgccagc
     1441 aaagggctca gccaccgcct gaaacaggct ggtccagccc acctctttta catcacgtcc
     1501 tgatttctgg ttttttcggg ttcgtcgcaa aaagttccac attcgctatg ctccgcatca
     1561 tgtttctttt tctgacccgc cggacgtcgc gctgtgatgt actccgcctt cctcaggcga
     1621 accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg ggcatcatgc
     1681 gtgccctgca gatatcggat ctttgccata acctgttacg ggaggcgcac gcctcccgtc
     1741 ctccttatca aactcagccg ccggacgcac tgccgtagtt cactccggtg atcaccgcca
     1801 ccgccgcggt acggcgacga cgccagttga tccagcgctc cgcacggatg gccacactgc
     1861 ctgtctggaa catggaaacc agctccaccg gcgacggcgt ggtactgtcg ccggtcggct
     1921 cagactgcat ttccagtgat gcctcgcggg acatatccac tgccacgccg ccgtcatccg
     1981 ccagataaat atccggggca ttcaccagca ccagctggtc acccacgtac tgggagacaa
     2041 tcaccggcag cccctggaag gagccaccca gcagggtcat gtccgggtat tctttctgac
     2101 ccagcgcatt tttacgcatg gacagcgcca gggcattggt gctggacatc agccagacag
     2161 caccggtggg ctgcaggttt gctgccacaa actgtccaaa cgccgcctct gcatccgcat
     2221 ccgggttacc gcttgatgcc gtgcccttca catcatgggt gatggacccg gggagacatc
     2281 tgccaccgcc gcttttttcg ggtccacaaa ttctgtatcc agacgcgcca ccaccgcttc
     2341 cgccagcgca ttacggacca gtgcatcagc agccggactg gaaaaacgga tcaattcttc
     2401 cgtcagtacc gcaatggccg acaccttcgc atgactgaag gtgatggatt caaaatcaaa
     2461 cttcgtcagg ggttttgcct taccctcacc cacccagccg gcagcaccgc ccgacacctg
     2521 ggcgtgcaca cggatattga acggcacctg acgaagtgca gggatcccgc cctgaccaaa
     2581 tcgcccgata atggtctgag gacgcaggta atcaataaag tcctgtgcgt attcctgata
     2641 ttcagacagg ctgcctgccc actgcggatc cgtggtggtc cccgcgccca ctgccgattt
     2701 caggacatga tgcagacgac tgtcatccgg atactgacga cgggccactt ccagggcttc
     2761 agagcggaca cctttagccg cagccagcga tttggcaaaa cgtgcgaaac caatcccctt
     2821 atccagtttc tgctccacac ggatcaccgg cgcagaagcc accgcggcca cattcccgtt
     2881 accggcctgt ttcaccggct gcgccgtggc ggccttaccg gcttccagtt cacgcaggcg
     2941 cttcaggtgc gcatccacct gacgtatttc cgctgcggtg ttgtcgtaat gctcttcctc
     3001 ctccacatcc agcgtgcgcc cttcctctgc ggctttggtc atgacctcct caagggaggc
     3061 tgccagtgct gcacgcttgt tttcaaaact tttaatctgt tcgccaatat tcattatggt
     3121 cttttcctta tgaaaaacgg ttgttgactg tgccgcagcg ccggcagaag atgcgatttt
     3181 caccaccggt ttccggttgc cggacgcggc agaaaactgg cggtcgtaag atttaatggt
     3241 ccggatggtg cattccgcat tcgcgggcac ggtgacggca gacacctcca tcagctccca
     3301 gcgcagaaaa tgcagtccgc ctccgtccag aaaggtgtat tcatgaggac ggaagcccac
     3361 ggacagcccc ttgaccagcc cggtcttaat ggccgcccag gcctcatcca gccgggcagc
     3421 cagttgcgat ggcatatccg gcacgggctt cgccagtgtt gccgtgattt ccagcccttc
     3481 gctgacccgg cgcaccgtac actgccccac cgggcgggaa tggtcatgct gccagagaaa
     3541 cgggatcgca ctgccaaact ccgcgccctc cggctccagg atgtcaccat cccgatccgg
     3601 agaaggcgtt gacgcaatcc cggtgatcac ccgctcatcc tcactgaagg atttcaccgt
     3661 cagcagggag caggcccgtt taagagtcac atcagcctcc tgaaaataaa aaaaccgccg
     3721 cagcggtcca tgatggttac agggtgaaca gggttatatg aaaaaaaccg catattcttt
     3781 ctttttcggc tccggattca gggacatcag ggataccgca ttgaagagcg ccatcagcgg
     3841 gtcaattttc ccccgcccgc tggcctgttt ggtaataagg atagcgttac ctttaggctc
     3901 cacccgggca ttgccaacgc accaggccat cagtggctga ccaccatgca ccagtactcc
     3961 ctcagccagt ttgcgctcgg tggtttttat ggccccgccc agcttccagc cctggcttat
     4021 ccccacaaca attccgtcgg ggatcccggc ttccgccagt gaatccagaa tctgccccac
     4081 acctgacggg tcaataccga tatgctccag taactcagcc tcatgaatac gacgcacata
     4141 ctccgccact tccgccgtgt catccccgac ccgacggaca atcgtcatgt ctccacaggc
     4201 cacaaaatcc tgaaaacggg atgcctcact cttccgtctg accaccgcgg tttcatgcgc
     4261 ccaggcatgg ccccagccca gccattcgcg ggtttccctg tcacggccaa tcacgtacat
     4321 tcccagcaga tcatccaggc ccccgccgtc aatccccacc gtcaccacat cagcgcgctg
     4381 caggatatcg tccaggctga cgcgcctgcc ctgctgctcc cagaaatccg cacccgccca
     4441 gcggtcagaa cgcagggcaa gaccaatttc cacattggca tgttttgaca tgaagccacg
     4501 aaatgcttcc tcaccagcct cccgggcttt acggtactcc cggtacagaa aagcctcatc
     4561 caccgaataa ccgagattcg gattgaccat ggcgaggttt tccatcagca ggtgagcccc
     4621 gctttccacc atttcaggag ggtgttcaaa tatcaccggc agaaagtgcg gatcatgaat
     4681 tttgccgtca cggacatccc gggcgtactg cagtttctgt ctgaacaccc cggctggcgg
     4741 ctcattcgac tgggtggtcg tatacaccac aaacccttcc gggcgggagg caaggccgcc
     4801 tatggcttca cgtaacatgt cctccgcttt gtactgcttg ccaaataacc acagttcatc
     4861 aatcagcgtc cccacggact tgataccgga taccgtattc ggatcggcag ccaccacctt
     4921 aagggtggtg tccgtcaccc tgtgggtgat ggtccggata tgtgtctgca cctgacagag
     4981 gtcatccaga tcatcgtccc gtcgtaccat atccctagca gggttgaagg cgttagccgc
     5041 cacctccacg gtcggggcca gaatggtgta gcccgccgcc tgccgccagt tcagtaacag
     5101 cgccgtcatc atgatcccgg cagccagcgt ggacttgctg tttttcttgg ggataaggat
     5161 aaaaacttcc ttgatatggc gtacaccggt ctgcgcaccg taggagccaa acagggccgc
     5221 caccaggtca aacacccact gtgcgcagga ctccccgaac gtcgggctgc ccggtgcatc
     5281 cacaatccgc agttgtttaa aaatcgccag ggcatgtgcg gcctgctccg gataaatcgg
     5341 agccggaata atcgacagcc cctttttcag gcgctctgcc cagtccgggc aggccgtgct
     5401 ccacacaggt atcatccgtt gccctcatta tcattattca ccaccaggcg gggtggtggt
     5461 ggcaccgcaa aacggttagc cgcttttttc gccgcgtcac cttttgccga ttttttaccg
     5521 gcatcccctt ttttgtggtg cgtgaactgc gccagcttat aagccgcatc cagcgccagc
     5581 ctggggtcgg tattaatgtt ctccaccaga agacgcccca tcgctttcac cggatcggga
     5641 agaccgtcct ccatatactc aataccagga gatatcacca cgggcggtgg catctccgga
     5701 tttgtttcgt ccggctgtgg tattgcagcc gcctcacggc gacggggttt atcctccggc
     5761 tctgattttt tctgccgata aacaggaacc tcatccacct ccaccgtctc gcactgttta
     5821 cgggctataa acgcaagcac ctccggatct tttgcctgct gcgagccttt aaccctggct
     5881 gtcttcgccg aataaccggc ggcaagggct gacgctgttt tgtttttccc ggacatgagc
     5941 gccagcgcaa attttcgttt ttgcgttgtc agcacagcct cctcccgggt ccagaacgca
     6001 ctcagccggg tatggttcag cccatttttc ccggcgtctc atgccgcaaa tgttaactgc
     6061 tgcctggtta acatttgctg aaaaatcctg ttaacatttt ttccacgcaa caaactgaat
     6121 aataaagata aaaaccgcaa aaatgcccgg acagccagtt aacatgttaa ctgccctgaa
     6181 acgggaattt tttctctgcg tgagaggggg cgcggtgtcc ggagcgatcg ttttttacgc
     6241 cgaatgatac ccccccggtc gggttacagt ccgatgatgt cgtccgctct gccactacct
     6301 ccggacacct ccggcagcgt cgtgtccggc ataccacccg ccgcttcacg agcagacttt
     6361 tgtcgatggc attcggtaca gagcgtccag agattcgtct cctcattacc accaccgaac
     6421 tgaagtgcaa ttcggtgatc gagttcactg tcacagaggt caaccacacg accacagaga
     6481 cggcactgcc cggcgtccct gagccagata tgatgcttga gggaaacacg tgcactgcca
     6541 ctgacacgac gctgttcacc cttcagaata ttcacccgtc gggtattcag tgttttgatt
     6601 ctgctctgga gtgtacgaag ctcagccatg taaaatcccc gtcatatggc aatcagtaaa
     6661 ggaaataaat atgtcatcga aaaaccgtac ccgcagaacc acaacccgca atatccgttt
     6721 ccccaatcac atgattgaac agatcaacat cgcccttgag cataaagggt ccggtaactt
     6781 ttcagcgtgg gttattgaag cctgcaggag aaggctggca acagatgcaa cgcatctgcg
     6841 cccggccagc atgacaaata acgagaaatg aacgttcggt tacaggagca ggtacccact
     6901 gtcctccaac aatatttcat cttcatatcc gacggaacaa gacttaccct gccgggatgt
     6961 acagaataac aacagagtga taattaattt ctgatgaaat aatcagggtg cagaaggact
     7021 aaagataaac gttttcttca cgcctttaca cggcctgtcc ttctcaaatc gccattttgc
     7081 catcgccttt acaacctgct catcaaacag atggtgcggc tctgaacgaa taaactcaat
     7141 tcgggtgaca gtaccatcag caccaatatc aaacctcaca tcaacccgtc cctttatata
     7201 atttgccgct gcataggccg gatattgtgg taatgcctta accaactgtc ggggcatatc
     7261 tgttttatgt tgcgtacagc ccataaccag agaagacaac aaaataatta acggaagatt
     7321 tcttttcatt ttcattcccc gcacagataa gaataagtct tattctaaca atgccgccct
     7381 gtcggtcatc aatcctctgc ttaatggcaa cgacaattat ccgacttaaa tcacaaatca
     7441 gacacatgac ataacagggc ttgcgaggta acacatcgtc ctgtttcttc caccatcgca
     7501 ccggactggc gactatgagg ggacaacgcc gcgctccgtt aacgcggtaa acc
//
LOCUS       DADKXM010000160         7527 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.163, whole genome shotgun sequence.
ACCESSION   DADKXM010000160 DADKXM010000000
VERSION     DADKXM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.163"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            556..1326
                     /locus_tag="JQR05_003514"
     CDS             556..1326
                     /locus_tag="JQR05_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY5325561.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="JQR05_003515"
     CDS             1317..1790
                     /locus_tag="JQR05_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY5325562.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="JQR05_003516"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="JQR05_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY5325563.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="JQR05_003517"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="JQR05_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY5325564.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="JQR05_003518"
     CDS             3225..3719
                     /locus_tag="JQR05_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY5325565.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="JQR05_003519"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="JQR05_003519"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY5325566.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /locus_tag="JQR05_003520"
     CDS             complement(6402..7424)
                     /locus_tag="JQR05_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY5325567.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
BASE COUNT         1913 a         1950 c         1833 g         1831 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ctgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgtttt
//
LOCUS       DADKXM010000161         7452 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.164, whole genome shotgun sequence.
ACCESSION   DADKXM010000161 DADKXM010000000
VERSION     DADKXM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.164"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            125..1531
                     /gene="fliD"
                     /locus_tag="JQR05_003521"
     CDS             125..1531
                     /gene="fliD"
                     /locus_tag="JQR05_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY5325568.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     ALAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKNGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1556..1966
                     /gene="fliS"
                     /locus_tag="JQR05_003522"
     CDS             1556..1966
                     /gene="fliS"
                     /locus_tag="JQR05_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY5325569.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            1966..2331
                     /gene="fliT"
                     /locus_tag="JQR05_003523"
     CDS             1966..2331
                     /gene="fliT"
                     /locus_tag="JQR05_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY5325570.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2409..3896
                     /gene="amyA"
                     /locus_tag="JQR05_003524"
     CDS             2409..3896
                     /gene="amyA"
                     /locus_tag="JQR05_003524"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY5325571.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3930..4343)
                     /locus_tag="JQR05_003525"
     CDS             complement(3930..4343)
                     /locus_tag="JQR05_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5325572.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4530..5735
                     /gene="yedE"
                     /locus_tag="JQR05_003526"
     CDS             4530..5735
                     /gene="yedE"
                     /locus_tag="JQR05_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY5325573.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            5732..5965
                     /gene="yedF"
                     /locus_tag="JQR05_003527"
     CDS             5732..5965
                     /gene="yedF"
                     /locus_tag="JQR05_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY5325574.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            6072..6742
                     /locus_tag="JQR05_003528"
                     /pseudo
     CDS             6072..6742
                     /locus_tag="JQR05_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6782..7452)
                     /gene="tnpB"
                     /locus_tag="JQR05_003529"
                     /pseudo
     CDS             complement(6782..7452)
                     /gene="tnpB"
                     /locus_tag="JQR05_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
BASE COUNT         1816 a         1924 c         1916 g         1796 t
ORIGIN      
        1 cgaaatactc ttttctctgc cccttattcc cgctattaaa aaaacaatta aacgtaaact
       61 ttgcgcaatt caggccgata accccggtat tcgttttacg tgtcgaaaga taaaaggaaa
      121 tcgcatggca agtatttcat cgctgggagt cgggtcaggt ctggatttaa gttcaatcct
      181 tgatagcctc accgccgcgc aaaaagcgac gctaacccct atttcaaatc agcaatcgtc
      241 gtttaccgct aaacttagcg cctacggtac gctgaaaagc gcgctgacga ctttccagac
      301 cgccaatact gcattgtcta aagccgatct tttttccgcc accagcacca ccagcagcac
      361 caccgcgttc agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt
      421 cacccatctg gcgctggcgc aaaccctgac cacgcgcacc accagagatg atacgaaaac
      481 ggcgatcgcc accagcgaca gcaaactcac cattcaacaa ggcggcgaca aagatccgat
      541 taccattgat atcagcgcgg ctaactcatc gttaagcggg atccgtgatg ccatcaacaa
      601 cgcaaaagcg ggcgtaagtg cgagcatcat taacgtgggt aacggtgaat atcgtctgtc
      661 agtcacatca aatgacaccg gccttgataa tgcgatgaca ctctcggtca gcggtgatga
      721 tgcgctacaa agttttatgg gctatgacgc cagtgccagc agcaacggta tggaggtctc
      781 ggtcgccgcc cagaatgcgc agctgacggt caacaacgtc gccatcgaga acagcagcaa
      841 cactatcagc gacgcgctgg aaaacatcac cctgaacctg aacgatgtca ccacgggcaa
      901 ccagacgcta accatcactc aggacacctc caaagcgcaa acggcgatta aagactgggt
      961 gaatgcctat aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga
     1021 tgcgggagct gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg
     1081 gacgattcag acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa
     1141 aacgctggcg cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc
     1201 cgacaaactc accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg
     1261 cgatggtaaa aaaaacggca tcacaaccac catcggcagc aacctgacca gttggctttc
     1321 gacaacgggc attattaaag ccgctaccga tggcgttagt aaaaccctga ataagttaac
     1381 taaagactac aacgccgcca gcgatcgcat tgatgcgcag gtcgcgcgct acaaagaaca
     1441 atttacccaa ctggacgttt taatgacctc gttaaacagc accagcagct acttaacgca
     1501 gcagttcgaa aacaacagta attccaagta atcattattc atcgggagac aggtcatgta
     1561 cgcggcaaaa ggcacccagg cctatgcaca aattggcgtc gaaagcgccg taatgagcgc
     1621 cagccaacag cagttggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc
     1681 gcgactgttt atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat
     1741 caacatcatt gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaattaac
     1801 ccaaaactta attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg
     1861 caacgatgtc tccgcagtcg aagaggtgga agcattaatg cgtaatattg ccgatgcctg
     1921 gaaagagtct ttactctccc cttcttcgat tcaggaccca gtctgatgaa caatgcaccg
     1981 catttgtatt tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca
     2041 acggaagaac aatgggacga actcatcgct agcgaaatgg cgtatgtgaa tgcggtgcag
     2101 gagatcgcac acctgactga agaggttgcc ccgtccacta cgatgcagga gcagctccgc
     2161 ccgatgctgc acctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg
     2221 atggatgaac tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc
     2281 tatggcgatc agggcggctt tgtgctggct ccgcaggata acctcttttg aatcttaatg
     2341 agccgatggc tcgcgaataa tccgattacg gctacgcttc taatattccc cttgaatgga
     2401 gtcgaagaat gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg
     2461 gtaagctctg gcctgaactg gccgagcgcg cagacggttt taatgatatt ggtatcaata
     2521 tggtctggtt gccgcccgcc tataaaggcg catcgggcgg ctattcggtc ggctacgact
     2581 cctatgattt atttgatcta ggtgagtttg atcagaaagg cagcatccct actaaatatg
     2641 gtgataaagc acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc
     2701 tgctggacgt ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc
     2761 agcgtgtaaa tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag tgtgaaggct
     2821 ggacgcgtta caccttcccc gctcgtgccg ggcaatactc gcagtttatc tgggatttca
     2881 aatgttttag cggtatcgac catatcgaac accctgatga agatggcatt tttaaaattg
     2941 ttaacgacta taccggagaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg
     3001 attatctgat gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat
     3061 actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca
     3121 aacatattcc ggcatggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa
     3181 agccgctgtt tattgtggct gaatactggt cgcatgaagt cgataaactg caaacgtata
     3241 ttgatcaggt ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg
     3301 aagcatcgcg catgggtcgc gactacgaca tgacgcagat cttcactggg acgctggtgg
     3361 aagccgatcc ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag
     3421 ccctcgaagc accggtcgaa ccgtggttta aaccgctggc gtatgcctta attttgttgc
     3481 gggaaaatgg cgttccttcg gtgttctatc cggacctcta cggtgcacat tacgaagatg
     3541 tcggtggtga cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt
     3601 taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga
     3661 actgcattgc ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt
     3721 cgaacggaga tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct
     3781 ggcgtgattt tctggggaac cggcaagaga gcgtagtgac agacgaaaac ggcgaagcga
     3841 ccttcttttg caacggcggc agcgtcagcg tgtgggttat cgaagaggtg atttaaatcc
     3901 atccccggcg gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta
     3961 tccagcgcgg cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg
     4021 tactcaatgg tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta
     4081 gtgagatcat cagacatcaa tgtcaactta cccggcacgg caatcacccg ctgccactga
     4141 cggcaatcca gggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccggactg
     4201 accagcgcac gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg
     4261 gttttcacga cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg
     4321 cctgcaattg cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca
     4381 ttaaagctct gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat
     4441 ttttgtgaat tattaccccg aaaataaatt tgcatggtgt atgattcgcg gctcttgttt
     4501 caaaccttct gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt
     4561 tgattaaatt ctgggcaccc atccctgcgg tcatcgcggc gggtattctc tccacttact
     4621 attttggcat tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc
     4681 agctcctgca gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc
     4741 tggaaggatc gccattaacc cgcatcgacg gcatgatgat cctcggtatg tttggcggct
     4801 gctttgctgc tgcgctgtgg gccaacaatg tcaaactacg catgccacgc agccgtatcc
     4861 gcattatgca ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg
     4921 gctgtaacct ggcggcgttc tttaccggca ttcctcagtt ctcgctgcat gcctggttct
     4981 ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct
     5041 tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg
     5101 atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg
     5161 cgctgctcac ggcgatgaac caaccaaaac tggggctggc aatgctgttt ggcgtcggct
     5221 ttggtttact gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga
     5281 tcaccggacg tacccatatg gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg
     5341 ggatcttcag ttacgtacag ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg
     5401 cggtaattgg tggtttactt tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg
     5461 gctggatgta ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaacg
     5521 tgatcggctc aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg
     5581 actgggacaa aatcaacctg ctgaaaactt ttggtccgat gggaggcctg ctggtgacat
     5641 atttgctgct gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc
     5701 gtgcggcacc gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct
     5761 tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca
     5821 attaaaaaaa ggtgagatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat
     5881 tccactggat gcgcgtaatc acggctacac ggtgctggat attcagcaag acgggccgac
     5941 cattcgttat ttaattcaga agtaatccct cattcccgct gtggcccaca gcgggaacat
     6001 cgttacctct gacacataga ttttacccgc cccctctcct attcttttca gtatgatctc
     6061 aggaggttgc tatgtgtgga cgctttgccc aatcccaaac gcgtgaggat taccttgcgc
     6121 ttctcgcgga agatattgaa cgcgatattc cctacgatcc cgaacccatt ggcagataca
     6181 atgtcgcgcc gggaaccaaa gttcttctgc tcagtgaacg tgatgaacac cttcatctgg
     6241 atccggtttt ctggagatat gctcccggat ggtgggataa accgccgctg attaacgccc
     6301 gcgtagaaac tgcctcaacc agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa
     6361 tctgttttgc cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa agccttattt
     6421 tatctatcgc gctgacggac aacctgtttt tatggccgcg ataggtagca taccgtttga
     6481 gcgtggtgac gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt
     6541 agatattcat gaccgccgcc cgctggttct gtcgccagaa gctgcacggg aatggatgcg
     6601 gcaagacatt ggtggtaaag aagcctcaga aatcgctacc agaagctgtg tgccagcaaa
     6661 ccagttcacc tggcaccctg tgtcgcgcgc ggtgggtaat gtcaaaaacc agggcgcgga
     6721 gttaattcaa cctgtttgtt gacatcctcc acgccctgaa tgatgaggac ccctgctacg
     6781 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
     6841 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta
     6901 catcggcgtt cgctatatat ccacatacct ggcatctgaa tttactttat gacaggcgat
     6961 tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag
     7021 caagcacctg accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc
     7081 agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct
     7141 ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta
     7201 tcattacgtg gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat
     7261 ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga
     7321 atttgacctt gcggcttaac tgtcgctgaa gtctcgccag cttcttctgg tttttctgaa
     7381 aactgtttac aggctcaaag actgtgccat ctgacagcga ggcaagttta gccacgccag
     7441 catccagccc ga
//
LOCUS       DADKXM010000162         7451 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.165, whole genome shotgun sequence.
ACCESSION   DADKXM010000162 DADKXM010000000
VERSION     DADKXM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.165"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            8..313
                     /locus_tag="JQR05_003530"
     CDS             8..313
                     /locus_tag="JQR05_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325575.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(389..853)
                     /locus_tag="JQR05_003531"
     CDS             complement(389..853)
                     /locus_tag="JQR05_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY5325576.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEADYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(850..1725)
                     /locus_tag="JQR05_003532"
     CDS             complement(850..1725)
                     /locus_tag="JQR05_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY5325577.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1722..2411)
                     /locus_tag="JQR05_003533"
     CDS             complement(1722..2411)
                     /locus_tag="JQR05_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY5325578.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2459..3949)
                     /gene="nanT"
                     /locus_tag="JQR05_003534"
     CDS             complement(2459..3949)
                     /gene="nanT"
                     /locus_tag="JQR05_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY5325579.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4059..4952)
                     /gene="nanA"
                     /locus_tag="JQR05_003535"
     CDS             complement(4059..4952)
                     /gene="nanA"
                     /locus_tag="JQR05_003535"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY5325580.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5074..5865)
                     /gene="nanR"
                     /locus_tag="JQR05_003536"
     CDS             complement(5074..5865)
                     /gene="nanR"
                     /locus_tag="JQR05_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY5325581.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            6245..>7451
                     /gene="dcuC"
                     /locus_tag="JQR05_003537"
     CDS             6245..>7451
                     /gene="dcuC"
                     /locus_tag="JQR05_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY5325582.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHF"
BASE COUNT         1836 a         1974 c         1877 g         1764 t
ORIGIN      
        1 agttgccatg atttacattg aacagcttga attaatccat aaaagtggtg atgtgttgta
       61 tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc ttccaccttg ttccattcgg
      121 cttaaataag actcacgacc ttctagaggt ggaagatgct tcagaagcaa ttcggctggt
      181 gattgatgaa cgtcattcca ttcgctgcag tacacttaca gcaactatta cgaataaaaa
      241 gggcaagcga attaagcgga caggtatata cagtatcaag ggtgttaaca taaaagaata
      301 caacgtcagg taatggcaca gagcggtccg tcagttcagg tttggctctg tgccaaagat
      361 gggtcaggtc acacctgatc taataaattt aagccattaa atcagcctta accttcacca
      421 ccaccttttt aatctcaccc ggctcgccaa caacgcatcc cggcttatgc ggctctcctg
      481 gcataaacac ggcgaacatt cccggtttca ggatgatggt ttgctcgttt tcaatggcgc
      541 tacaaagctg gtaatcagct tcatggtgga actcttcaca ttggcgcgca gtgcctgcca
      601 tgccaaacag aatccgttcc tcaccgttta ataacagctg gatatcaatg tattgctcgt
      661 gcaattccgc ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaaa
      721 tattgtcgcc ctgtaattcg taacgacccg gcgctttttc ttgcggtctg gcagctaagg
      781 ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc agacggtaat gactgtactt
      841 cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc
      901 atggcggtaa tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata
      961 catttccacc aacgccagat acccttctgc cagaccaacg ctgccaccaa ccaccacgca
     1021 ctggcaatca gttgtggctt taatatcagc gatcagcctt gcaagcgtac gtgcggagcg
     1081 atgaatcagt tgctgcgcct gctcatcgcc ctgcccggcg cgtgtgaaaa tagttctcgc
     1141 atccgcgcca gccagctcgc cctgcgctgc cgccgcaatg ccgcgaccag aagcaattgc
     1201 ttccacgcaa cctgtgcgtc cacagccgca gactgggccg tgtggatcgg caagcgtatg
     1261 cccgatgtgc cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc
     1321 gccgccgacg ccagtggaaa cggtgataaa gaccatatcg gttatatcgc cttccagcgc
     1381 ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt
     1441 aagttgttcc agcgttttga ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt
     1501 cagcgccagc aaactgccgt cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc
     1561 atgagcttgc aacggagaga ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg
     1621 gctggctggc gtaggaagtt cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc
     1681 ggcggcaagt ttagtaccgc cgatatcaat cgccagtatg gtcatagcac cgcctttttc
     1741 atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc
     1801 accgcccacg cgccgtggcg catcgcatct gccgcctgag caggcgtgtt gtaacgccct
     1861 tcggcaatca cccgacatcc ggcttcgctc aacgttttca ccagcgccag atccggctct
     1921 tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc
     1981 ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga
     2041 tgatggatac gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca
     2101 atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat ataggccgtg
     2161 atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc caataatcgg cacgctcacc
     2221 accgcacgcg tggcttgcag atttgccaca ccttcgatgc gaatggcaac cgcgcccgcc
     2281 tgctctgccg ctaatgccat ggctgcgacg atttcgggtt tatcgagcgg gctgtcagga
     2341 accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca
     2401 agtaacgaca tacatcttcc cttagcgaaa ggcccggtac atagaccggg caacaggatt
     2461 aacttttggt tttgactaaa tcgtttttgg cgctgccaaa tggcacggca ccgctaaatg
     2521 gtttgccgtc gatagcgtca tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc
     2581 gggaaggcat atccagacca atcagcagga tcaccacaaa tgtcaggctg aacgagagcg
     2641 atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca
     2701 gtgcgccacc caatgcgcca acgttgtagg taaagcccag gcccgctgca cgctggtcgg
     2761 tatcgaaata accgccaatc agtttcggta agatcccggc gatcccttgt ccaagcattt
     2821 gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg
     2881 gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc
     2941 agtcaccgag gaagccacct acgcagcatc ccacggccgc gccaaagcca ctaaagaaca
     3001 gcacattggc gacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca
     3061 gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc
     3121 ccgttggcca gcgtttaccc gtactttgca ccataaagct gataaagatt gcggcgcata
     3181 acagcccaag aacagcgacg attgcagcat tttgcaggtt accggcgaag cagaaccaca
     3241 gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga
     3301 gaatatccac cattgtgcgt actggtgctt tacctgcgtg tttctctttc cagtcttccg
     3361 cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga
     3421 acagcgcacg ccaaccccag accggaacca ccaggctata gacctgagcg gcaacgacgg
     3481 cccccacaga gaagcctgaa atcaaaaaac cactggcttt gttacgcaga tgttttggcc
     3541 agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga
     3601 ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg
     3661 ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag
     3721 cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg
     3781 cctgcaccgt cgtcagcccg aattcaccct gtacttcggt gagtaccagg gcgattaaaa
     3841 cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcg cgccattgtg
     3901 cacggttgag atggcgatac cacgggatat tctgggttgt agtactcatt gtgagtctcc
     3961 cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt
     4021 tcccctcacc cggtaggggc gagcgagggg aaaacaactc acccgcgctc ttgcatcaac
     4081 tgctgggcca gcgccttcag ttctggcaga tatttttcat ctaccggtcc aaacggtttg
     4141 cggcacagcg gcacagaaac gacatccata taatgcagga cagttttcag gccgcggaat
     4201 acgcccgttt tgatcagtaa atcaatgact ttgttgcact cagtttgcag tttctgcgcg
     4261 gtctggatat cgccttcttt cagcgccttc acgatcccct gatatcgcca gcccatgatg
     4321 ttgtaagtac tgccgatacc accatcagcg cctgccagca agccggaagc gaagatttcg
     4381 tcgtaaccgt tatagagcac cagatcagga tgttcacgac ggatctgttc catctgatag
     4441 agatcgccag aagtctgttt cagcgcaccg acgccaggca acgtaaccag ggtgttgatc
     4501 tgatccaggg tcagttttac cccactcagg gctggaatgt tgtacaccac catcggcaaa
     4561 ccatccgctg aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga
     4621 tagtagaacg gcgtgacggc agagacggca tcgaagccat aacgtttagc cgatgccgca
     4681 agttgttggc tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct
     4741 ttcgcctctt cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag
     4801 gcttcgccgg tcgaaccacc cacgtataaa ccgtcgatgc cctgctgaat attgaactga
     4861 accaggcggc gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt
     4921 gcagccatta cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga
     4981 taaacgatat acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa
     5041 gcttataatg cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc
     5101 gaaagcgtgc caggtagcag agacgctgtt gagatgcgat tgcaacgcac gatcggcttc
     5161 gtcaggatca tgacggcgga tcgcatcaac gatcgcaata tgctgttgat aactaacgtt
     5221 gttatgttcg tgcagtgcct gatcggtaac cgttgggcgt gcggcaataa gccagtcgag
     5281 cagggcaacg tggatcgcca tgaagattgg gttaccgggg atctccgcca gcacgcggtg
     5341 gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc
     5401 cagtgctttt gccagcaaat caatttgctc atcggtggcg tgttcagccg catagcgcac
     5461 caggctggat tcaaagaaca gacgtaattg ttcgaaatgg gcaatcccac caggatgaga
     5521 aaggaaatct ttcgccatgc cggaaagctc gccgatgatg gtgtccgcag aaggtcgaga
     5581 gacacgagcg cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag
     5641 cgcttcacgc acagaaggac gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg
     5701 taattgttca ccttcgccaa attcacgacg gcggatcatc tgttccagct cttcttccac
     5761 catttcggag agttttttac gcgccagcgg gcggctacgc aagttgcgac caattgcagg
     5821 tgaagaatct tcggtttgcg aatcaaatgc attcataagg cccattctgt aaggtcagtg
     5881 tgattaacat catcagtgac atcctatcac aggattgaaa gtaggggaaa atggcaggtt
     5941 tttctctttg tgcctcatca ttaccataat taacggaata attaactatt gcgaaaaatt
     6001 aatgtaacgc agataaacac atcccgattg aattattcat aagactattc acaagcatta
     6061 tgaatattat gaatgtgttc ttacaaaata atcataatcg catatttttt aatgaaaaat
     6121 cacctcacct acaattaaaa acacgatatc cgcaccataa atagacttgc aaaaaatata
     6181 acatcattgt tttcaatctg ccgtttatgg gattgaccgt tttcttttga cacggagttc
     6241 aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct atttgatcct
     6301 gaaaaactac aaacctcagg tggtgctggc tgccgcaggt gtcttcctga tgatgtgcgg
     6361 agtctggtta gggttcggtg gtgtactcga tcccgccaaa agcagcggct acttgatcgt
     6421 cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat tggggctgtc
     6481 gattatggcg gtgggcggtt atgcccgcta catggagcgc ataggagcca gtcgcgcgat
     6541 ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg ccgtatatta ttctgtcggc
     6601 aacttacgtc atcggccaaa tcatggcgca gtttattacc agcgcctccg gtctgggtat
     6661 gttgctgatg gtcaccttat ttccgacgct ggtgagtctg ggagtaagtc gcctctctgc
     6721 ggtggcggtt atcgcaacca cgatgtccat tgagtggggg attctggaaa cgaactccat
     6781 ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac ttcttccact accagcttcc
     6841 ggtcgcctct tgcgtcatta tctcggtggc gatctcccac tttttcgtgc aacgcgcttt
     6901 tgacaaaaaa gataaaaata tcaatcacga acaggcagag caaaaaactc tcgataatgt
     6961 cccgccgctc tattacgcca ttttacctgt gatgccgtta atcctgatgc tcggctcgct
     7021 gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat ctggtggtgg tgatgttact
     7081 aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag cataacttgc gcgaaacaat
     7141 ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgtcaacg tggtaacgct
     7201 ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg ttgatgcggt
     7261 tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta ttatggcgct
     7321 agtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta tgtcatttgc
     7381 cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa tgattatgcc
     7441 gatgcatttt g
//
LOCUS       DADKXM010000163         7382 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.166, whole genome shotgun sequence.
ACCESSION   DADKXM010000163 DADKXM010000000
VERSION     DADKXM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.166"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(199..1452)
                     /locus_tag="JQR05_003538"
     CDS             complement(199..1452)
                     /locus_tag="JQR05_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325583.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTHYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1559..2452
                     /locus_tag="JQR05_003539"
     CDS             1559..2452
                     /locus_tag="JQR05_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325584.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2587..3807
                     /gene="mlc"
                     /locus_tag="JQR05_003540"
                     /gene_synonym="dgsA"
     CDS             2587..3807
                     /gene="mlc"
                     /locus_tag="JQR05_003540"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY5325585.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3932..4627
                     /gene="bioD"
                     /locus_tag="JQR05_003541"
     CDS             3932..4627
                     /gene="bioD"
                     /locus_tag="JQR05_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY5325586.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4580..5872)
                     /gene="clcB"
                     /locus_tag="JQR05_003542"
     CDS             complement(4580..5872)
                     /gene="clcB"
                     /locus_tag="JQR05_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY5325587.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6031..6645)
                     /gene="dmsD"
                     /locus_tag="JQR05_003543"
     CDS             complement(6031..6645)
                     /gene="dmsD"
                     /locus_tag="JQR05_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY5325588.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6688..>7382)
                     /locus_tag="JQR05_003544"
     CDS             complement(6688..>7382)
                     /locus_tag="JQR05_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY5325589.1"
                     /translation="GIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGG
                     LWWLVAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLT
                     VLLSGPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQAS
                     ALVPDYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFY
                     GLHMTVGMAIAG"
BASE COUNT         1970 a         1874 c         1934 g         1604 t
ORIGIN      
        1 agcggtagaa taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt
       61 tgggtactta taatcctgat gacgctagat gcgctgcggg aattggctgg cgcttcgtct
      121 attttaggat ggctattaac gttggtttga gcaggcaata agtccggacg ggtatttacc
      181 gcagtccgga cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag
      241 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
      301 gaaaacacca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
      361 cgaggaggcc tggcctttag cgcgttttgc gcgggggccg atccagctgc tggctactga
      421 gtgggctgcg aagaatccgg ctgagaagag taacattccg gcaaagatca gccacagcga
      481 gctaaataag gtcatcagta aaccaaacag cataactccc gtcgaaaaca acatcaccgg
      541 accgcgccca tagtgggtgg tcatggttcc ggctttgggt gagctccatg taccggtcaa
      601 ataagccagc gataataagc caaccacggc ctgactgaca tgccaggggg aaagcatcaa
      661 ccgatagccg atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc
      721 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa ataacgtctt
      781 agggcgcagc gaagtcgggc aaaaatggcg tgattcaggg aggattttcc agaacatcaa
      841 cgccgaggcc agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc
      901 cgtgaagaca ccactaatta agcgtccgct catgccgcca attgagttgc cgctgatata
      961 caaccccatc gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc
     1021 aacagctgcc acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg
     1081 ccagctggtc atcattgtcg aaagtaacgt acaaatggag gccaacagta gcgccgtgac
     1141 catcactggt ttgcgaccaa tggcatcgga tagcgggcca gtaaatagca aaccgatagc
     1201 caacatcgcc gtggaaatgg acagtgaaat actactgttc gcgggcgtta agccaaactc
     1261 ctgcgaaagc accggaagga taggctgcac acaatagaga agtgcaaagg ttgccagtcc
     1321 ggcagagaac agcgccaggg tgacgcgcat aaattgcggc gtaccgcgtt taataaattg
     1381 atttggctga gaaatgcttt gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt
     1441 agtacggctc acttgaaatc cttgctaaat atgcctgtgg atcaggctta tacatagggt
     1501 aggaaaatcg aattgttctg tctaatatat taataatctc aaataagatg ttttaaatat
     1561 gaatattgaa cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcattttgg
     1621 gcgcgcggct gcccgcctga atatttcgca accgccgcta agtcagcaga ttcaggcact
     1681 ggagcaacaa atcggtgccc gactgctggc acgaactaat cgcagtgtat tgctgacggc
     1741 agcaggaaaa cagtttcttg cagatagtcg gcaaatcctg tctatggtgg atgacgctgc
     1801 cgctcgcgct gaaagactgc atcagggtga agcgggggag ttgcgtattg gttttacttc
     1861 gtccgctcct tttattcggg cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc
     1921 tgatgtccat ttacaaaccc gcgaaatgaa cactcgcgag caaatcgctc cgctcattga
     1981 aggaacgctg gatatgggat tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc
     2041 agtcatcgtc catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa
     2101 tccgagtgta acactggctg aactggcgaa agaacccttt gtcttttttg atccgcacgt
     2161 cgggactggg ctgtatgacg atattctcgg actgatgcga cgttaccatt tgacgcccgt
     2221 catcactcag gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg
     2281 tgtttcaatt ttgcctgcgt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc
     2341 gattgctgaa gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca
     2401 aagtccagct gcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt
     2461 tcagcgaaaa agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac
     2521 acgtattgaa gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag
     2581 tatgcggtgg ttgctgaaaa ccagcctggg cacattgatc aaataaagca gaccaacgcg
     2641 ggcgcggttt atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt
     2701 ctggcgcagc tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac
     2761 ctggtgcgag agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg
     2821 gtggttgaaa ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc
     2881 cttgctctgc gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta
     2941 aaagatgact tgccattgct ggatcgtatc atttcccata tcgatcagtt ttttatccgc
     3001 caccagaaaa aacttgagcg tctaacttcg attgccataa ccctgccggg aattattgat
     3061 acggaaaatg gtattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc
     3121 ggcgaggcgc tggagcagca taccggcgta ccggtttata ttcagcatga tatcagcgca
     3181 tggacgatgg cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg
     3241 gttatcgatc acaacgtggg ggtgggcgtc attaccgatg gtcatctgct acacgcaggc
     3301 agcagtagtc tcgtggaaat aggccacaca caggtcgacc catatggtaa acgctgttat
     3361 tgcgggaacc acggctgcct cgaaaccatc gccagtgtgg acagtattct tgagctggca
     3421 cagctgcgtc tcaatcaatc catgagctcg atgttacatg ggcaaccgtt aaccgtggac
     3481 tcattgtgtc aggcggcatt gcgcggcgat ctactggcaa aagacatcat tatcggggtg
     3541 ggcgcgcatg tagggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata
     3601 ctgattggct caccgttaag taaagcggca gatatcctct tcccggtcat ctcagacagc
     3661 atccgtcagc aggcccttcc tgcgtatagt cagcacatta gcgttgagag tactcagttt
     3721 tctaaccagg gcacgatggc aggcgctgca ctggtaaaag acgcgatgta taacggttct
     3781 ttgttgattc gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta
     3841 tctcaatttg ggccaggaaa gcataactta gactttcaag gttaattatt ttcctagttt
     3901 atatttgtga agcataacgg tggagttagt gatgctgaag cgtttcttta ttaccggtac
     3961 agacacttct gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca
     4021 gggaaaaacg gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg
     4081 gctgcgtaat aaagatgcct tggtgttgca gagtgtttca accatcgaac tgccttatga
     4141 agcagttaat cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat
     4201 caattacacc ctcatttcaa acggcctggc aaacctgacc gataaagtcg atcatgtcgt
     4261 ggtagaaggg actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg
     4321 ggtagtgcag gaacaactgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa
     4381 ccatgcactg ctaacagctc aggcgatcgc caacgacggg ctgccgctca ttggctgggt
     4441 ggctaaccga atcaacccag gactggcgca ttatgcggaa atcattgatg tgctgggtaa
     4501 aaaacttccg gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgtga
     4561 actggggcaa tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac
     4621 ggtgtaacgt ccgcgaaatt accgacgcaa ttacgcaggc aatcaataaa ccggggagta
     4681 gctgatactc cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg
     4741 ttgccgccaa cagtgtcgcc atcccggtca atccgagtaa aagcgtaatt tcttcgccat
     4801 cagggaacca taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata
     4861 gcgtcggtgt aaagacacca ccgggtgcgc cggaaccgct actcgccagc acggcacaca
     4921 gtttacaaag gaagatcccg gcaatgatca ttaacagtgg tggggcggtt aaaaaggatt
     4981 gtacggtgct atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga
     5041 tcaacccgcc tagtgccagt tgccagggcg gcgcaagttt gagactcaca aatccacgat
     5101 gacaggcgtt cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac
     5161 tgataatcag cgcatagtca cgagcctgaa ccgtcactga gagttgtacg ttgtagagta
     5221 acgcgtcgct atgattaatc agattgctaa ccagcaatgc cacgacggcg gaaataatca
     5281 ccgggccgag agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc
     5341 cagcaagcgg ggcacgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata
     5401 atttccattc ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta
     5461 aaagaatcat cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagcgagg
     5521 caagcgattt aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca
     5581 tgtaatcggt cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca
     5641 aacccgccgc cagtccgccg agcgccggag ttagcagccg tcgccagggg gaaaggtttg
     5701 tcgctgcatt gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca
     5761 tcgcatgacg aaacccggca acggcaaaga ccgcgagaat accgacgact gttgcgataa
     5821 gcagacggcg gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa
     5881 atgggcaatg aaacacgggc gactattttg cggcaaaaga ggccaaccgc aaaagcagtg
     5941 cgtgctttaa gagataaaaa aacgtgacat tgtaacgtat tattgccgaa tcgcgcgtaa
     6001 tcgtcaccat ccggcaatat tacggtgatc ctatcgaaag agtggtttaa ccgcgacagg
     6061 aatcaacagt tgtgattgcc attgcgccag cgttaagcga gccagttcac ccagtgcacg
     6121 gtagaaaggg tgttctgctt tttcgataaa aacatcaaga aaacgtgttg accacggaaa
     6181 aaggtgccat gccagcaatt cttcgcattc tgtctggcga ccattctccg ccaaccacgc
     6241 agccatcaac agcagtgaac caaaatgatc ttccggttcg ttttgcttca tttcaaactg
     6301 aatgcctttc tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa acagcacaga
     6361 ttcgcgatcc agccagaccg acccccacgg cggagacggc agtgcccacg ggccgacaaa
     6421 caaacgctgc caggcctggg cgtgagtctc ttcacactgt gtctgaaaag ccgtcaccag
     6481 cggcgctaat gacgcttctg gtagaggcca ctgagtttcc cagccgtcac tggtgagtac
     6541 cgcaaccaga ggtgcggctt ccgcactctc tggagcgtaa taaaacagcg cgcccaacac
     6601 gcgcgccgcg acagaaaaat tatcttgctg tgaaaaatgg gtcattccta acatcctgaa
     6661 atgtgcgggt tgccccgcac caatttgtta acctgcaata gccattccta ctgtcatgtg
     6721 caggccataa aagagcacac gcccgatcat ctcaccaaca agtatgagga ttagccccag
     6781 aacaagtccg gcaacgtgag gttcacggcg acgaatgagc ggacaaagcc agcatccaag
     6841 acctgcgcat aacaatacca cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc
     6901 gctggcttgt tgcacggatg agtgaataga cgccagagac aaaccttgca gcacaatcac
     6961 ctcggcacaa gcgatcagcg ccagaacgct aatgatggca aatggtgtgg tattaaatgt
     7021 aacgcgggct gcccgcagaa ttgcggcagc aagtattgga ccgctcaaca ataccgtcag
     7081 gaagaaagcc agcgtagtgt aaccgttatg ccaggttggc acggtgtcga tttgatacac
     7141 gcaggtcatc atccagacga aaatgacgcc gagcgccatg ctgacaagta accagagttt
     7201 ccccaacgct tgcggcattt taccgatgac cgccaccagc caccatagtc cgccaacggc
     7261 aaaaaatatg gaaccggcag caatctcgtt actcagacca gaagctccga ttcgattcag
     7321 cgagttaaac gctcgcagtg gcgatcccag gtgcatgaca gaggcaataa acccgatgcc
     7381 ca
//
LOCUS       DADKXM010000164         7252 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.167, whole genome shotgun sequence.
ACCESSION   DADKXM010000164 DADKXM010000000
VERSION     DADKXM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.167"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..185)
                     /locus_tag="JQR05_003545"
                     /pseudo
     CDS             complement(1..185)
                     /locus_tag="JQR05_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            263..1357
                     /locus_tag="JQR05_003546"
     CDS             263..1357
                     /locus_tag="JQR05_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY5325590.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1381..1593)
                     /locus_tag="JQR05_003547"
     CDS             complement(1381..1593)
                     /locus_tag="JQR05_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY5325591.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGGLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1853..2161
                     /locus_tag="JQR05_003548"
     CDS             1853..2161
                     /locus_tag="JQR05_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY5325592.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2220..3314)
                     /gene="yaiW"
                     /locus_tag="JQR05_003549"
     CDS             complement(2220..3314)
                     /gene="yaiW"
                     /locus_tag="JQR05_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY5325593.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3327..4547)
                     /gene="sbmA"
                     /locus_tag="JQR05_003550"
     CDS             complement(3327..4547)
                     /gene="sbmA"
                     /locus_tag="JQR05_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY5325594.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4899..6056
                     /locus_tag="JQR05_003551"
     CDS             4899..6056
                     /locus_tag="JQR05_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY5325595.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6057..6560)
                     /locus_tag="JQR05_003552"
     CDS             complement(6057..6560)
                     /locus_tag="JQR05_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5325596.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6651..7175)
                     /locus_tag="JQR05_003553"
                     /pseudo
     CDS             complement(6651..7175)
                     /locus_tag="JQR05_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1838 a         1850 c         1748 g         1816 t
ORIGIN      
        1 aaagctatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
       61 tgccatttaa gctaggaata aattagcttc ccgctcatca tttttgtgat cttgcgcaca
      121 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
      181 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
      241 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
      301 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
      361 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
      421 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
      481 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
      541 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
      601 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
      661 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
      721 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
      781 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
      841 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
      901 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
      961 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     1021 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     1081 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     1141 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     1201 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     1261 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctggagcg
     1321 tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc gataatattc
     1381 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     1441 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     1501 cagtcccccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     1561 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     1621 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     1681 atttaactta aggttgccat tttgtgactc acaacgaaga ttcacgcttc gggaatattc
     1741 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     1801 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     1861 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     1921 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     1981 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     2041 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagc
     2101 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     2161 aaatttacgt tgccagatgc ggcctgaaac gccttatctg gcctgacatt cctgtttgat
     2221 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     2281 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     2341 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtatagcgc cgtctcctca
     2401 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     2461 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     2521 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     2581 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     2641 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     2701 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     2761 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     2821 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     2881 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     2941 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     3001 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     3061 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     3121 tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     3181 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     3241 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     3301 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     3361 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     3421 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     3481 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     3541 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     3601 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     3661 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     3721 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagcaat
     3781 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     3841 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     3901 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     3961 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     4021 gccatgtaat attcgttcat cgccgtacgc cagcggaaca cgtagtgact gacaaagaag
     4081 ttgttcagta cactgatcac caccgcgatt agcgcaatcc ccagaaagac gcccacttcg
     4141 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcagatca
     4201 tagaacggcg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaagtgacg
     4261 aagataatca gcgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     4321 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     4381 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     4441 gccacccagt cacccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     4501 gccgagagaa aaaacgttcc cggctttggg aaaaaagact taaacatatg cactcctgct
     4561 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccgctac aaagcgtcag
     4621 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     4681 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     4741 caggatcaca aatgcaggca ttggtgtgca ggaaaaagat cgtgtttacg cgccgttacg
     4801 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     4861 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     4921 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     4981 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     5041 ccacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     5101 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     5161 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     5221 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     5281 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     5341 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     5401 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     5461 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     5521 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     5581 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     5641 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     5701 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     5761 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     5821 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     5881 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     5941 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     6001 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     6061 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     6121 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     6181 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     6241 aataatgttg cgcgggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     6301 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataagga
     6361 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccttctaaa
     6421 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     6481 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
     6541 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
     6601 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ctaggtatat
     6661 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
     6721 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
     6781 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
     6841 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
     6901 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
     6961 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaatgctg
     7021 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg
     7081 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
     7141 gacaggtcgt tgttgaagtg agagtagctc aagctatgaa tcgccacgga taatctagac
     7201 acttccgagc cgttgataat actggttttc atattctgtc ggtgacatct gt
//
LOCUS       DADKXM010000165         7131 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.168, whole genome shotgun sequence.
ACCESSION   DADKXM010000165 DADKXM010000000
VERSION     DADKXM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.168"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JQR05_003554"
     CDS             complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JQR05_003554"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY5325597.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLSFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JQR05_003555"
     CDS             complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JQR05_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY5325598.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2428..3192
                     /gene="ybfF"
                     /locus_tag="JQR05_003556"
     CDS             2428..3192
                     /gene="ybfF"
                     /locus_tag="JQR05_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5325599.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3263..3625
                     /gene="ybfE"
                     /locus_tag="JQR05_003557"
     CDS             3263..3625
                     /gene="ybfE"
                     /locus_tag="JQR05_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY5325600.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            3765..4295
                     /gene="fldA"
                     /locus_tag="JQR05_003558"
     CDS             3765..4295
                     /gene="fldA"
                     /locus_tag="JQR05_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY5325601.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4505..4591
                     /gene="uof"
                     /locus_tag="JQR05_003559"
     CDS             4505..4591
                     /gene="uof"
                     /locus_tag="JQR05_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY5325602.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4584..5030
                     /gene="fur"
                     /locus_tag="JQR05_003560"
     CDS             4584..5030
                     /gene="fur"
                     /locus_tag="JQR05_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY5325603.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5114..5440)
                     /locus_tag="JQR05_003561"
     CDS             complement(5114..5440)
                     /locus_tag="JQR05_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325604.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JQR05_003562"
     CDS             complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JQR05_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY5325605.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1729 a         1824 c         1776 g         1802 t
ORIGIN      
        1 cttgcgaccg cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa
       61 tctcaaccgc ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt
      121 agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca
      181 tcactttcag accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga
      241 tcgggtcacc tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg
      301 ctttttgtgc ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt
      361 ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg
      421 ccagcagaca cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca
      481 ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat
      541 caacaaacca tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca
      601 cacggtcgat catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact
      661 gcggacgatg ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca
      721 aacctgccgg agtgacgata ccgtgacggt cataatccgg gtcgttagca aacgacagat
      781 cgaacttatc acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac
      841 ggatcgcacc gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa
      901 cgatggtcag gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac
      961 cgcccaacgg atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga
     1021 tatcggccag cccttccacg aacggctgca ccagatcctg ctctttcaca tggccggatg
     1081 ccatcgcttc gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt
     1141 tggccctgtc ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat
     1201 tgtacttgat accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg
     1261 ccagcgggcc acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag
     1321 tgaagccatt gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta
     1381 cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag
     1441 tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct
     1501 cgttaaagct gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt
     1561 gctccgcatt ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt
     1621 taatcaaatc actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt
     1681 cctttgtctg caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat
     1741 tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg
     1801 atcacccaat acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc
     1861 agcgtttgtt catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct
     1921 tcggcaaacg cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg
     1981 accgctcgct tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc
     2041 ttgtctttaa tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc
     2101 tctttcgtca ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa
     2161 atgtcggatg cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca
     2221 tcatcaactt caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg
     2281 ccaagagtat atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac
     2341 tcggaataaa ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc
     2401 cgacaacgcg aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa
     2461 aaccagcaca ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac
     2521 cttggcgtac tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt
     2581 aaccacggtc tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt
     2641 gttgataccc tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc
     2701 ggtaaagcgg taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg
     2761 atcgatatcg caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc
     2821 aacgcggtca gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag
     2881 catcttaatg aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg
     2941 cgctttaacg tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc
     3001 ccggcatggg atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag
     3061 cagtaccgtg atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc
     3121 gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat
     3181 ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtggtc
     3241 gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg
     3301 gctgattgtt tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac
     3361 attagatctg ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg
     3421 cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct
     3481 taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga
     3541 atcgcgtaat atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc
     3601 gctgcgtagt cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg
     3661 ctcgtttgct gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa
     3721 ttttccaccc ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca
     3781 tctttttcgg cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc
     3841 ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag
     3901 cttacgacat tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact
     3961 gggatgactt cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt
     4021 ttggttgtgg tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc
     4081 gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc
     4141 atttcgaagc atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg
     4201 aagaccgtca gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg
     4261 aagagttgca tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct
     4321 acgccgtatt aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg
     4381 ttgtacctgt acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc
     4441 cacgttttta ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc
     4501 tataatgata cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg
     4561 cttagtaaca ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc
     4621 tgaaagtaac gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc
     4681 acgtcagtgc ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg
     4741 ctacggtata tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt
     4801 ttgaaggcgg taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt
     4861 gcctcgactg cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg
     4921 aaattgccgc aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact
     4981 gtgccgaagg cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga
     5041 acgagaaaag ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag
     5101 ttacagctct cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg
     5161 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta
     5221 cgcgtacact ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta
     5281 acacgctctg gcaggcttca attttttctg gcgaaccttg cgcggtgttg atacaggcgc
     5341 tgtatgcctc tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca
     5401 gccccgatgc catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa
     5461 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
     5521 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     5581 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     5641 agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat
     5701 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     5761 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     5821 tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
     5881 aggaagcgta ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa
     5941 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
     6001 cttgcagcca ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
     6061 gggtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
     6121 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
     6181 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg
     6241 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
     6301 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
     6361 ccgccccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg
     6421 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
     6481 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
     6541 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
     6601 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt
     6661 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
     6721 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
     6781 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa
     6841 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
     6901 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg
     6961 ttgagaaaag tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc
     7021 tctttctgta accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt
     7081 acaaggctta agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga a
//
LOCUS       DADKXM010000166         6951 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.169, whole genome shotgun sequence.
ACCESSION   DADKXM010000166 DADKXM010000000
VERSION     DADKXM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.169"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            76..1023
                     /locus_tag="JQR05_003563"
     CDS             76..1023
                     /locus_tag="JQR05_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY5325606.1"
                     /translation="MLDAYDADEISETSYINKLRRLAQWEPDFIDIHAHLAYAFLEQN
                     APRKALNAALKGLAAGNRIIPESFCGEIIWMHPENRPYLRALYAAILANVHLQRHQDA
                     VMLTDKILAYNPEDNQGARWLLGSELLRTGDHERAFSVLKEHADEFSPYWYELGLLHF
                     LNGEHVKAATAFRHGFATNTYIAEMLCGNLHPFPLAVWHDFSGSLDTAEDYYATYSPL
                     WGQYPEALLFVNWLYNHSSVLHERSEIIKCAEMLIQEDDFEICESILRQQEHLWKRID
                     KTLSEEIVQKCRNMNGEYIWPWILPFSAAGMKHSSIQYQ"
     gene            1538..1999
                     /locus_tag="JQR05_003564"
     CDS             1538..1999
                     /locus_tag="JQR05_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325607.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(1996..2851)
                     /locus_tag="JQR05_003565"
                     /pseudo
     CDS             complement(1996..2851)
                     /locus_tag="JQR05_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2848..3147)
                     /locus_tag="JQR05_003566"
     CDS             complement(2848..3147)
                     /locus_tag="JQR05_003566"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325608.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            3609..3866
                     /locus_tag="JQR05_003567"
     CDS             3609..3866
                     /locus_tag="JQR05_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325609.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(4507..4779)
                     /locus_tag="JQR05_003568"
     CDS             complement(4507..4779)
                     /locus_tag="JQR05_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325610.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            4868..5047
                     /locus_tag="JQR05_003569"
     CDS             4868..5047
                     /locus_tag="JQR05_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325611.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            5099..5293
                     /locus_tag="JQR05_003570"
     CDS             5099..5293
                     /locus_tag="JQR05_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325612.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(6092..6428)
                     /locus_tag="JQR05_003571"
                     /pseudo
     CDS             complement(6092..6428)
                     /locus_tag="JQR05_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2009 a         1568 c         1476 g         1898 t
ORIGIN      
        1 gaagtgcacg ataacaaggg ttatttcgta tttccggata cctggtttgg cccattgctg
       61 ggggagtttg aagaagtgct tgatgcatac gacgctgatg aaatcagcga gacgagttac
      121 atcaacaaac tccgacgcct tgctcaatgg gagccggact ttattgatat acacgctcat
      181 ctggcatatg catttctgga acagaatgcc ccgcgtaagg cactgaacgc cgctcttaaa
      241 ggactggcgg caggtaatcg tatcatcccg gaaagtttct gtggtgaaat aatctggatg
      301 catcccgaaa accggcctta tctgcgcgcg ctttatgcag caattctcgc aaatgtgcac
      361 ttacagcgcc atcaggatgc ggttatgctc actgataaaa tactggcata caatcctgaa
      421 gataatcagg gggctcgctg gttgttgggt tcagagctgt tacgcaccgg cgatcatgag
      481 cgagccttca gtgtactgaa agagcatgct gatgaatttt caccttactg gtatgaactc
      541 ggtctcctgc attttctgaa cggggaacat gttaaggccg ccacagcttt tcgccatggc
      601 tttgccacaa acacttacat cgcagaaatg ctgtgcggaa atctgcatcc atttccactt
      661 gctgtgtggc atgacttctc cggctctctg gatacggcag aagattacta tgcaacatac
      721 tcaccattat ggggacaata tccggaagca ttgctgtttg taaactggct ctataaccat
      781 tcctccgtgc tgcatgaacg atcagaaatc attaaatgcg ccgaaatgct gattcaggaa
      841 gatgatttcg aaatatgcga aagtatttta agacagcagg agcacctgtg gaaaagaatt
      901 gacaagacac tttctgagga aattgtacag aaatgcagaa atatgaacgg tgaatatatc
      961 tggccctgga tattgccctt ttccgcagca gggatgaaac attcaagcat ccagtatcag
     1021 tagatattgc attagtgtaa ctttaaaaca agtaataatt tattatccaa taataacact
     1081 gtgttccttc cccaagtgat taccctgggg ttattttctg aaagtttcct ttgcaaaagg
     1141 atagcgttgt aaattacttt aacgaattac tccatgaact cattaaagtt ctttcgtaat
     1201 actttatcgt caaacacgtt atagctggca tcaaaaacca cagatttacc ctgccagcgg
     1261 gtaatcccct cctctggcgt tttcggttga actggtatcc ccgtccaaca caggcagcca
     1321 ttataaacag taaaaaattg tattcgctgt tatgaaatca atagtcctga cgtactgttg
     1381 aatgtgcttg tgttctgaac ctcgtacaac cagtacattt gatatcgaat tataaacaaa
     1441 acattgcatc caactttttt aatatggaca atagttgtcc atacggtggt tttatattcg
     1501 ataaatcaat ggatttcatt gagtcacagg actggtaatg gatgaaaatg ctttaaggtt
     1561 tgcctcatac tggcgcaact cgcttgcaga tgctgagtca ggaaagggct gttttgaacg
     1621 gaaagacgcc caaaatttca ctcactggca tgggatagcg gcgggacgtc ttgacgaagc
     1681 gattgtcaat aaatttttta agggagaaaa agacgatgtc gaaactgtca atgtcatctt
     1741 acgcccaaaa gtttatttcc ggttactgca gcatggtaag gaccgttccg caggcacgcc
     1801 tgatattgtt acaccgatag tgacgccagc cttgctaagc cgtgagggtt ttttatatcc
     1861 gacgccagcg acctccattc ccagagacct gcttgaacct ttgccaaaag gagcattttc
     1921 gattggtgag attgggcagt atgacaaata caagacgacc catactgatc ctacccacgt
     1981 aatgtggaca cggccctaag cgagattatg gttttcaaat tgttccgggc tgagaccgcc
     2041 acaggcactg tgacgacgcc agcgattgta atcacactcg atataattaa acaccgttgc
     2101 ccgcattatt tcccggctgc taaagcgttc cccgtggata cattccacct tcagcgaatg
     2161 aaagaagctt tccacacagg cattgtcata acagtagcct ttcgcactca tactgccacg
     2221 caggttgtgt cgcttcagca ttgcctgata atcccctgaa cagtattgac caccacggtc
     2281 cgtatgaaca atgacgcttt ccgggcgtct tctccgccac aacgccattt gcagtgcatc
     2341 acaggccagt tgtgctgtca ttcgcggtga catcgaccag ccaatgacgg cgcgtgacca
     2401 caggtcgatg actactgcga gatacaacca gccctcatcg gtacgcaagt acgtgatgtc
     2461 acccgcccac ttctggtttc gggccactgg cgctgaagtc ctgctccagc agattctcca
     2521 atacgggcag gccatgtgca cggtagctga ccgggctgaa cttccggctg gctttcgccc
     2581 gcagcccctg acgacgcagg ctggcggcaa tggttttaat attgaactcc ggcagttcgt
     2641 cagcaaggcg gggagcaccg tatcgctgct ttgcctcaaa gaatgcctta tggacagcgg
     2701 catcgcaggt gagccgaaac tgttggcgca ggctcatctg gtgacgacgc ctgagccaga
     2761 cataccagcc gctgcgggca acccgaagta cacgacacat cgctttgatg ctgaactctg
     2821 cccgatgatt ttcgataaag acatacttca tttcaggcgc ttcgcgaagt atgtcgcggc
     2881 cttttggagg atggacagtt cctcagcctg ctccgccagt tgtcgtttaa ggcggacatt
     2941 ttcagcggcc agttcgcttt cgcgctctga cgaactcatt tgttgctgct gtttactgcg
     3001 ccaggcataa agctgagatt catacaggct gagttcacgg gctgcggcgg ccacaccgat
     3061 gcgttcagcg agtttcaggg cttcgttacg aaattcaggc gtatgttgtt tacggggctt
     3121 cttgctgatt gatactggtt ttgtcatgag tcacctctgg ttgagagttt actcacttag
     3181 tcctgtgtcc actattggtg ggtaagatca ccctccatta aaactaaaac gggcgctttc
     3241 tgatatatca tctgtaagcg cagtaatacc attagtagca cctctcggcg gcgcattgat
     3301 tattgaatat tataactgga atgttatatt tcttgttatg atgctgttca gtattataat
     3361 aatgttatta tcaccaaaga taactcatgc aaatgagaaa gtaatatgtc aaaacagtgc
     3421 atcctttgtg accaaagagt ttatctgcgg cactatactt gtctcattaa tactcagttc
     3481 tctattttgc tacatatcaa tatcaccatc aattttcatg catacttatg gcctgagtac
     3541 ttttagttac tcaattattt tctccttctc agttttattt ttcataatcg gaaatcaatt
     3601 atcaaagatt gaaaaattat ccaacctatg gtttttatta cccaatagcc aattttatcg
     3661 cattacaaat aaatggttca aaaacaggat ttgccgcatc tattacaggt tttactcaaa
     3721 caataagcgc tggtacaatc agtttcctgt cagtaaccgt aagcgacaac ggtgttggtc
     3781 ccgggactac tttaggtctt ggttcactaa taacttcatt actgtcaata gccgtaatcg
     3841 ccattgcagg aaataataaa aagtaagtgt actctgactg cgtatgtatc tactttgtgt
     3901 aatgcaggtg tctattgaaa ataattcttt tcgtgggcat ctgttgtata aaataaaaaa
     3961 ttatcgtaag tactccatct gcgatgtctt gtgaaaatta tcctgtctgg caacaatcgc
     4021 gtccatctgt attaatggac tcgaacgatg aactctcaaa caacactaga tatcaccggc
     4081 tttcgcactt atttgccaga atcattacgt ctcagattcg acgagcaatt atctgtccgg
     4141 gctatcgcac agcagttaag actcagttat tcccccattc acaatctctt tcaacgaatt
     4201 aacgcctcag gtattgtctg accatgaccc gagtcccttt cattcgccca accgatacca
     4261 tactttatgc caacagaaag aatgaatgaa ccgcgcctaa aatcagcgag gacacatggc
     4321 acaaagtgcg acatgtctca catttaaccc cagtgggggc aacaagtata aaaccgtttt
     4381 ttgattaagt ggtcggatat gaaaagggga aggtttatgt gaaaaatacc tgtgttttct
     4441 ttgaaaacac acaatagtat caggttattt atgtttatac agtgcgttcc aaactgacct
     4501 gaataattac gcaagttgca acaataaaac atttttgccg tagtgtgagg tactccggta
     4561 aattgctttc caggtgcaga caatgactaa cgaagaacta caggcaatcg cattatccat
     4621 gcttgagcgc cagcgcgaaa aagtacacca gcaacgagag tctttgctta acccatctcc
     4681 attcaccagg ccaaaacgat tggagcttga agcattcttg gacaaatacc cacggcggct
     4741 taaatcaggc aaaaccagac caccaggctg atagctcaca gtttgttctc cgattcaata
     4801 aaacggataa gaaccatcag cgcggtagcg taaggcacta cagcgcattg ctttacgagt
     4861 aataacaatg gatttatcac cagaagacgc aaagttttta cgagaactgc aacaccgtat
     4921 gctcagagaa gccattatcg aacagcaaac gggcatgagc gccatagacc cattgcgata
     4981 caaacgggcc tcatctcctt tagttgacga caattccaat gcggataaaa cacgtccacc
     5041 acactgatta catttttcat tagcacagtg gtgtgatgca gcatacgagg aaaaccggat
     5101 gaatgagaac aaaataaaaa ggctggagca actgttacag gcacgccaac gggagtttgc
     5161 cactaaatgg ggacgtgccg cgctgccaca tgaacgccta acctattttc cacttgaaga
     5221 cctcattaaa aataatggca aaaaaatcag gtctgataag aaacacttct tagaatgcaa
     5281 tgaagacaaa taacaacaat ctgcatattg ccaccaacca cagatgtggc atcgttatca
     5341 gcgcacttga ttttctttat atccactgac gagcttgccg gggcaccttt gtttgccggt
     5401 cattctggta tcatgcagat atttacggcg tacaagccca ctagcatgac attgtaataa
     5461 ggcctgagcc taacgcttta atgtgtgtca tacactcagt ggcgtaatcg ttgacaatat
     5521 ggtgcatgta atcgtaagcg tccagcgaaa tccatctggt cataatctga agcatccgac
     5581 aaagtggtgt ccaccaaata agtgatctgc gcccaatatc ctgagccgaa actttgacaa
     5641 aggccaacga aaaatgggag cggatgttac agagttctcg gtacagggga aaagtggtac
     5701 ctgtcgccag ttctccgatc tgtttaaccg ggaaattatc tcctacagct tgtcagaaag
     5761 gtcggtgatg gagcatcgtt aatacgatgc tatacgatgc attcacagtg cccggccaga
     5821 ggatgccccg tcgctgcata tggatcaggg ttggcaatat cgaatcgcag gctataggca
     5881 aagttaaagc cccatggagt agcacaaaat atgccgcaca aaggaaacgg tctgaataac
     5941 gcagtgatga agaacttctt cagcacactg ctaaaacaca tagtgatcga gcgctgattc
     6001 tggcgaacaa ctgaactaat acatcagaat ctgcattatg ttaaataaat atgaaaagat
     6061 ggtttaaata ccccgttact tttgacttac actatacggt atcgcatcgt ttaatattcg
     6121 caccggccag atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta
     6181 ttctggctcc tgtgtggtta tgctttaatt ctgcgttccg ggcagataag cagttgcttg
     6241 caggaatcct tcttgtgtta atgtcagttc ccctttttac cagtgctgat ttccacattc
     6301 cgtccaacag agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt
     6361 tgaataataa tagaagcact gctggcagat ccagtccgtt tttcataacc cactgtactg
     6421 ataaccataa tctaatcagt agaaattgag tcgaaaataa gcactactcc atacaggata
     6481 attagagatc agtttgatta ttcacaattc atcatcagca ttttctattt ctgacgaaat
     6541 caacatgaaa ataaccatat atgataatta ttataataac ggctttaatt ggaatacata
     6601 tattacaacg tattatatat aattggtatt ctgggaacta tattctcaaa atacagtaga
     6661 aacgaggtat gtttctggtg gaaaggacag ttggattaaa aagtaagaat tgataaaaaa
     6721 aaacgccagc aacacatagt gccggcggag ggaatacccc atggagaaaa tgtgatgcct
     6781 agagcatcat gaatatataa ttaaaaatag ttagcattgt caatactttc acaaaaataa
     6841 ttttcgtagt atagaaagat atttttatgc atgacctacc tgaatttgct ccgggtagag
     6901 gttataaata aaaattgaat cacgacaaac acaatattca tagtatggcg a
//
LOCUS       DADKXM010000167         6929 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.170, whole genome shotgun sequence.
ACCESSION   DADKXM010000167 DADKXM010000000
VERSION     DADKXM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.170"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(260..877)
                     /gene="tdk"
                     /locus_tag="JQR05_003572"
     CDS             complement(260..877)
                     /gene="tdk"
                     /locus_tag="JQR05_003572"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY5325613.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1482..1895
                     /gene="hns"
                     /locus_tag="JQR05_003573"
     CDS             1482..1895
                     /gene="hns"
                     /locus_tag="JQR05_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY5325614.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JQR05_003574"
     CDS             complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JQR05_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY5325615.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JQR05_003575"
     CDS             complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JQR05_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY5325616.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JQR05_003576"
     CDS             complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JQR05_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY5325617.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5272..5730
                     /locus_tag="JQR05_003577"
     CDS             5272..5730
                     /locus_tag="JQR05_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY5325618.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5780..6622
                     /gene="purU"
                     /locus_tag="JQR05_003578"
     CDS             5780..6622
                     /gene="purU"
                     /locus_tag="JQR05_003578"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY5325619.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6782..6866
                     /locus_tag="JQR05_003579"
     tRNA            6782..6866
                     /locus_tag="JQR05_003579"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6816..6818,aa:Tyr,seq:gta)
BASE COUNT         1889 a         1702 c         1508 g         1830 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaacccga cagcattagc cttcatcata
       61 tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa
      121 cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt
      181 atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
      241 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
      301 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
      361 caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
      421 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
      481 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
      541 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
      601 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac
      661 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
      721 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
      781 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
      841 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
      901 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
      961 agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg aactatcatt
     1021 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
     1081 acctgttgcg caagtaatag ccctctgttg acctccaaga gatagtgcaa tactaagtcc
     1141 atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
     1201 acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
     1261 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata
     1321 tgccgcgtct tttctggcta attttatgaa aatatattta ttggcggcac aaaataaaga
     1381 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
     1441 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     1501 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     1561 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     1621 ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa atgctgatcg
     1681 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
     1741 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     1801 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     1861 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     1921 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     1981 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     2041 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     2101 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     2161 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
     2221 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     2281 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
     2341 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     2401 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     2461 ccgtttcatc aaagcggcgg atcatctctg ctaggttatc ctgtgacaaa tcggattcat
     2521 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     2581 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
     2641 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     2701 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     2761 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     2821 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     2881 acatcctagt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     2941 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     3001 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gatagcataa
     3061 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
     3121 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     3181 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     3241 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     3301 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     3361 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
     3421 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     3481 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     3541 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     3601 gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg caatgggaaa
     3661 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     3721 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     3781 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     3841 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     3901 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     3961 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     4021 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     4081 aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     4141 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     4201 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     4261 ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc
     4321 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     4381 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccaacacc
     4441 tggatagacg tggtcataat ctctgttgcc gttggcgctg tcacagcccg acgtgtcgtt
     4501 atgctgccta acctttcttt cagacgcgca tgccacggta gagaatcacc attctcgcta
     4561 ttttcttcac tgacattaaa ggagagcaga tcttgttgca tcaaatgagc atcgtgctgc
     4621 aggtcgaccg ctatcacaat atcagccccc aatgcacgcg taagagaaat aggaattggg
     4681 ttaactactg ccccatcaac cagccagtag ccgttatgtg caacgggtgc catgagtcct
     4741 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     4801 cgccccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
     4861 gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag taacccaccg
     4921 cgctgccagg agaggtccat caggcgtaaa acgtcccaat aactgaaaga ggttacccaa
     4981 tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga accaattgaa
     5041 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
     5101 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     5161 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     5221 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     5281 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     5341 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     5401 caagacgctg attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
     5461 gccgaattga tgaccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
     5521 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
     5581 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     5641 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
     5701 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     5761 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     5821 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     5881 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
     5941 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     6001 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
     6061 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggtctgg atgtcgaaat
     6121 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
     6181 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
     6241 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
     6301 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
     6361 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
     6421 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
     6481 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
     6541 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggcg caacgcgtct ttgtttacgg
     6601 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
     6661 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
     6721 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
     6781 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     6841 aggttcgaat ccttccccca ccaccatcac tttcaaaagc cccggaattc tcaaacgaat
     6901 ctgcaatcaa atattctgcc caagcgggg
//
LOCUS       DADKXM010000168         6809 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.171, whole genome shotgun sequence.
ACCESSION   DADKXM010000168 DADKXM010000000
VERSION     DADKXM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.171"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..729
                     /locus_tag="JQR05_003580"
     CDS             <1..729
                     /locus_tag="JQR05_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5325620.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1032..1802)
                     /locus_tag="JQR05_003581"
     CDS             complement(1032..1802)
                     /locus_tag="JQR05_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325621.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1847..2281)
                     /locus_tag="JQR05_003582"
                     /pseudo
     CDS             complement(1847..2281)
                     /locus_tag="JQR05_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2293..2514)
                     /locus_tag="JQR05_003583"
     CDS             complement(2293..2514)
                     /locus_tag="JQR05_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325622.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2515..3198)
                     /locus_tag="JQR05_003584"
     CDS             complement(2515..3198)
                     /locus_tag="JQR05_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY5325623.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3262..3573)
                     /locus_tag="JQR05_003585"
     CDS             complement(3262..3573)
                     /locus_tag="JQR05_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325624.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3570..4541)
                     /locus_tag="JQR05_003586"
     CDS             complement(3570..4541)
                     /locus_tag="JQR05_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY5325625.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLKVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4912..5871
                     /locus_tag="JQR05_003587"
     CDS             4912..5871
                     /locus_tag="JQR05_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5325626.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAIIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQLALVKAMVHA"
     gene            5871..6266
                     /locus_tag="JQR05_003588"
     CDS             5871..6266
                     /locus_tag="JQR05_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5325627.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6449..>6809
                     /locus_tag="JQR05_003589"
     CDS             6449..>6809
                     /locus_tag="JQR05_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY5325628.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLNA
                     LYCCAKEKIGLLSDNRDA"
BASE COUNT         1860 a         1764 c         1658 g         1527 t
ORIGIN      
        1 gatgcagaag ccatatgtga agcggcttca cgaccatcaa tgcgttttgt ccagccaaga
       61 actgagaccc agcaggcaat aagggcatta caccgggtca gagaatcgct catcagagat
      121 aaggtgaaaa caaccaacca gatccacggt ttcctgctgg aatttggaat cagcctgccg
      181 acaggggatg ccgtaataaa acggttgtct ctcgtattgg ctgaacacga aatccccgaa
      241 tatctgagcc gccttttggt gaggttgcat acccactacc tttaccttgt tgagcaaata
      301 gcagagctgg aatcagaatt aagtcagtca atcaacgctg atgataccgc tcagcgcatt
      361 atgacaattc ctggtgttgg gccgattact gccagcttac tatcgtcgca acttggtgac
      421 gggaaacagt tttcatgcag cagagatttt gcggcttcca cagggctcgt tccccggcag
      481 tatagcactg gaggaaaatc gactctgttg ggtatcagta agcgaggtga taaaaattta
      541 cgccggttac tggttcagtg tgcccgctca ttcatgatgc agcttgaacg ccagcatgga
      601 aaacttgctg agtgggttcg ggagcaactc aacaagaagc attcgaatgt ggttgcctgc
      661 gctctggcaa ataaactggc ccgaattgcc tgggcaatca ctactcagca aaaagaatat
      721 caggcctgat atatagatgc tgacaaaact caattagctt attgcaaaca ataagataca
      781 agttaccgtc tggttttgcg aagattgatt attgatgaca tgaacggcaa atcgacctga
      841 ttaaaaacct aacaggggaa aatggctcac tgaagccgag gggattttta ggattatcag
      901 gtgcggaact catcgaggcg cgggcatccc ttgcccacag agacgccgga tagatttaag
      961 caagcccacc attaatcaaa aatcagtgtt gcaaaaacgg gggtgaccat agattttccc
     1021 cgccatcgtc atcatttccg gatttttgcc agggcatccg ccatgctgcg gatttcggcc
     1081 cgggcttccc ggacaacttc ccgcagaaca ccacggtcaa accaccggcg gggcggttca
     1141 tccggtatac gcagcacatc gcaggccagc ccactggaca ggatatgctc aatacaatcg
     1201 ctgaccagat acatttccac cgccgaaaaa cgtgcgcccc ggttcaggat ggcatcgagc
     1261 actacgggca ggttttcatc gtcttcatcg agccagcagg ggtgatcgct ttccttcacg
     1321 accagccgaa agcggcgcgt ctggcgggtc agcaacgtct gaccgcttgt ggtcagcgcc
     1381 gcccggatac gccgcgcatc ttctgccccc cggcaactga cactgcatac tgcatcgcgc
     1441 accgcatcaa gaactgccct gatttcgtct tcggtcagcc attcaccggc atacgggaaa
     1501 gacacctgcg ttccgtccgc cggaagtacc ggcagcgact gccactgact gccggacaga
     1561 acaagttccg ctccggttcc ggtcagtttc tgaatggcgg aggccaggtg cggcggcagc
     1621 acgccatcgt ggcggttaac gagtaacgtc gccagccggg gcgcatccag ccgcacgtaa
     1681 atgctgatcc agcccggata gttcgggtta cgttggtcaa catccgtctc ttccggccag
     1741 ccctgcgcca gcagcaggtt acgggcgtga cgacaaatca gagcattcag cgtttcattc
     1801 ataattcatg aactccctgt atcgccgccc gcaggcggcg ataacatcag tcgttttcgt
     1861 gtgtgttgtt tttcagcagg ctgcgggcct gatcaaccat cgcctgtcgg cgggtaacgt
     1921 attccgggag ggggaaaccg tgatcccggg cagcccattc acgtttccac agtgcctgtt
     1981 cagcgatctg agtcagggtc tccagtagtt cagcagggac ggtcacctgg cggttttcgc
     2041 gcacgttgct gataatctcc atcgccgcgg cggaagaaat accggactca atccgctagt
     2101 cttccgggat gtcctccagg tgcgccagga cggtcctgat ttcatgatcg gacagggaaa
     2161 tatccattcc gtcagcgagg gcctgaatct cttccgggga ccagataacc agcatcagcg
     2221 gcgtgtctcc cgaatacttt gctgccagcg cccggcatag cgtttcgcat gttccgtaca
     2281 tacactctcc cctcaatctt tgaagcagga atccagttcc agaatttcgg tatatgcctc
     2341 catcaagagg cggtttgcgc gaaacaacgt ctgacgcacc agacgttccg gcagggtgtc
     2401 agcattaaag ctgtaccgcc gctccatctc attcatggtt gtacgcaggt cgcacatggc
     2461 attggccacc tccgccactt cggcgcgaag tttttcgtgt ccggcatagt tcatttacgc
     2521 agcctccggc ataaaccagt catcattcgc ggccccctgc tgcatggccc gctgtacggc
     2581 agccagtcgt tgctgtccgg cacggtgata ctgctcaagc agctcgatac cgatataccg
     2641 gcgtccggac tggagggcgg cgacgcaggt tgaaccgctg cctgcaaacg ggtccagcac
     2701 tatcgcattc gggtgtgtga agctctcaat cagcggttgc aggctggtaa cgggcttttc
     2761 cgtcgggtga tggcggttgc ccgaatattt ccagcccaac acgtccggca gcgggttttg
     2821 tggcagacgt ggacggcctt ttgccaggat gtaggcgcat tcgtggcgat agcccacata
     2881 tgcggccttc gatgtgtagt ttttggtgaa gaccaggtga ccaacaacgc tgaatcccgc
     2941 atttttccag gcagacataa agcgatcgac gcggttccag ccatagaagc tcaccatcag
     3001 cgcgtctttt ttgagtacgc ggtacatttc attacaggcc ggttgcagcc attcatcagt
     3061 tttatcacca gcaatggtac gcccctgacg gtcacgaaaa ccgacgagat atggcggatc
     3121 ggtgaggatg aaatcaatgg cgttatcagg aatacgggcc ataacatcga tgcagttacc
     3181 gaggacaaaa cgggacataa cagatccttc tcttacagag ggcacccctg tggtgcccgt
     3241 tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata
     3301 ccgcagccgt cgtctttttg acagcaaggt tatgccgcga aagcggcttg aagggcgttc
     3361 atcagaaaag ggcgacgggc tggcgtacac tgtcaccgac gccagccggg agcggtaaaa
     3421 gcgcccgtcc cgtgcccgcc ggacgcggga cactgaccac aggcagcgtt tatgccgcgc
     3481 cgcgtcagcc ggcgcagtaa aaagaggagc acggcgacat cctttgtcgg aaaagaaacg
     3541 ggcggttgcc cgcccgtctc cgtcatactt catccgccat aatgcgccac attgtcgact
     3601 atccacgcag gtccggtgac ttccggctgc tcatcgtcat ctgtcgggga agaccattca
     3661 agttcccccc acagcacatc gacgagctct ccacgctcat acaactgcca gccacagaaa
     3721 tcacagccct gttcggcata ccagtgctcc agcgtgcagc tgaaacggcg gcttaattct
     3781 gcaataacgt cgctttccgg ctgacaccac ggcgtgtcga aatccacctg aatgaagttt
     3841 tctccctgac tgctgatatt caggtcatat ccacaaggcc acttcacgcc ataccgttcc
     3901 gtataccagt gatatgcaga aggaacaccg ttcagaacac caccattaaa gccgttcact
     3961 tcaacatcca ggcgggtcgg cagaaccttc agcaggtcaa acggcatggt tttttccggc
     4021 actacgttgt cacacagacg gttccaccac accgatacat cctcgttacc ccatatgtca
     4081 caccagtcac ctctttttgc ggtgaaatgc acagtgatgg tttccctggc atcatccggt
     4141 aatccttccc agcgacgctg gccagtaccg ctctgtaacc agagttcatg cagcatcagg
     4201 cagttttgtt catccagcaa cacaccgttc tgcaggtgtg tcagccagcg ggtgaacgcg
     4261 atattttccg tcgatacagc accacgtccg gcagccgtca ctccggggca ctgttcagag
     4321 cggatatttt cagtgatctg cagaagtccg gcacttccgg ccagaaacag ctgaatacct
     4381 tcatttgtgg cgcggcgata aagcggtgtg actgcaccgc tggccagtcg tttaatctca
     4441 gcgatctgtg ccggttcacc agaaaaatac atacgattcg agcaccagtt tggcatggga
     4501 attctcctct cacatgctgc ctccatcagg ccgtgccgca cctgtaaccg ggcgaccgca
     4561 tacgaatgca ggtgcggccc cgaagccgca gcgcgtggag gaggaacgac gaaacgtgac
     4621 cgggtgagga ggccggcgac agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc
     4681 ggcacggcct ggttaagtag tatccgtctc agttcgtggc gcaaaatgag aaaccaataa
     4741 gaactcaaaa tgaaccacaa caagaacata aagagactct aaaagagcaa caccgcttga
     4801 catagactca aaatgagtca caataagaaa caataaaaaa ctcaaaaaga tgcaccgaca
     4861 taaaggatgc tcaatttgag ataaacacaa actcaaaacg aggcaaaaag aatgctaaaa
     4921 gtatcatgcg atgatggttc aactaacgta aaactggcct ggcttgaaga tggcgaggta
     4981 agaaccagtc tgtcaggaaa cagctttaaa gaaggctgga acccgggact gtttaatgcc
     5041 ggcaaagttt acaattacgt cgtggatgaa aaaaaataca cctatgattt gggaagcact
     5101 gctatcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acaaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt ggcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttga cccccgactg ttaacgctaa
     6061 tcagcggttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gcaatctttc tatgttaaaa aaataataaa agaagagcag gttcagtcaa ttacctgctc
     6301 ttcttttata catactaaaa aatcagattt ttacgaatat tcagattatc ataaaaaatg
     6361 caacagacag gaatgcaata attttgcatt atctcgttgc ttgtaatgca aatatgcatt
     6421 attgtcagtg tgtgacaata acagagagat gacgatggcg accattaacg cacgtattga
     6481 tgatgatata aagaaccaag cagatgaagt gctgaagctg atgaatatca gtcaaactca
     6541 ggctattgca gctttttatc agtatattac tgaacaaaag aaactgccat ttgtgatcac
     6601 ctcaattgta aaaacgccgc atgatttatt acgtgaaagt acagacatgt tagctgaagc
     6661 tcttgcagtg atttccaatc tacaggtgtg gactgaacag caggatggga ttggaaaagc
     6721 aaagctaatg gagtattacc gccgcctcaa cgcactgtac tgttgtgcaa aagagaaaat
     6781 aggtcttctt tcagataacc gggatgctg
//
LOCUS       DADKXM010000169         6715 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.172, whole genome shotgun sequence.
ACCESSION   DADKXM010000169 DADKXM010000000
VERSION     DADKXM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.172"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            334..1989
                     /locus_tag="JQR05_003590"
     CDS             334..1989
                     /locus_tag="JQR05_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY5325629.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            2129..2353
                     /locus_tag="JQR05_003591"
     CDS             2129..2353
                     /locus_tag="JQR05_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY5325630.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            2416..2952
                     /gene="rimL"
                     /locus_tag="JQR05_003592"
                     /pseudo
     CDS             2416..2952
                     /gene="rimL"
                     /locus_tag="JQR05_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(2947..3927)
                     /locus_tag="JQR05_003593"
     CDS             complement(2947..3927)
                     /locus_tag="JQR05_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY5325631.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWLDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFSSVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            4051..5043
                     /gene="tehA"
                     /locus_tag="JQR05_003594"
     CDS             4051..5043
                     /gene="tehA"
                     /locus_tag="JQR05_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY5325632.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            5040..5633
                     /gene="tehB"
                     /locus_tag="JQR05_003595"
     CDS             5040..5633
                     /gene="tehB"
                     /locus_tag="JQR05_003595"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY5325633.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5936..6604
                     /locus_tag="JQR05_003596"
     CDS             5936..6604
                     /locus_tag="JQR05_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY5325634.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPAPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6622..>6715)
                     /locus_tag="JQR05_003597"
     CDS             complement(6622..>6715)
                     /locus_tag="JQR05_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY5325635.1"
                     /translation="QQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         1720 a         1556 c         1642 g         1797 t
ORIGIN      
        1 tgccagtcgc gaagcatttg agcaggcgtt gggttgtccg gttttggatg agttccagct
       61 ttaccaggaa gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat
      121 ccgcctttgc aaaaatttgc ctgattttac aaacgaatca ggctcatccc atcgacataa
      181 aaaaaatgcc gatttatgca tattctctca gttcaacaat tggattatta ataaatattg
      241 tctagagtga gcggtcataa ataagcactt tcttgccgct gaaaacgacc agcgcgggac
      301 cattcacaac accagaagga ctcactttca ggtatggatc gtagacgatt tattaaaggt
      361 tcaatggcta tggccgccgt gtgcggtacc agcggcattg cttctctttt ttctcaggcg
      421 gcattcgcgg cagattctga tattgccgac gggcaaaccc agcgttttga cttctccatt
      481 ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta
      541 cctgacacgc tggcgacaat gacgccgcag gcttataaca gtattcaata cgacgccgaa
      601 aaatcgctct ggcataacgt tgagaaccgt caactggacg ctcagttctt ccatatggga
      661 atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt
      721 gaaattcact ttcgcccgga gttgttcaaa tacaacgatg caggtgttga taccaaacaa
      781 ttagaagggc aaagcgatct cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg
      841 gcgcgccgtg atgtagtatc atttctcggc gcgagttatt tccgcgccgt tgatgataca
      901 tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa
      961 gagttccccg actttaccgc cttctggttt gatacggtaa aaccgggggc aactaccttt
     1021 accgtttatg cgttgctcga tagcgccagc attactggtg cctataagtt cactatccat
     1081 tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt
     1141 aaacagctgg gcattgcgcc gatgaccagt atgttcagct gcggtactaa tgaacgtcgg
     1201 atgtgcgata caattcatcc gcaaattcat gactctgatc gtctgtccat gtggcggggc
     1261 aatggcgagt ggatttgccg tccgctgaat aatccgcaaa aattgcagtt caatgcttac
     1321 accgacaaca acccgaaagg gtttggttta ttgcaactgg atcgtgactt ctcccattat
     1381 caggacatta tgggctggta taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag
     1441 tggggtaagg gcaccatcgg cctgatggaa atcccaacaa cgggcgaaac gctggataac
     1501 attgtctgct tctggcagcc agaaaaagct gtaaaagcag gtgatgagtt tgcattccag
     1561 tatcgtctgt actggagtgc gcaaccgcct gttcattgcc cattagcgcg cgttatggcg
     1621 acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa
     1681 aaatgggcgc gtcgttttgc cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca
     1741 aaaggcattg agccggtgat tacgctttcc agtggggaag cgaagcaaat cgaaattctc
     1801 tatattgaac ccatcgatgg ttatcgtatt cagtttgact ggtatccgac ttcggactcc
     1861 actgatccgg tcgatatgcg gatgtatcta cgttgtcagg gggacgctat cagtgaaaca
     1921 tggctgtatc agtatttccc gccagcgccg gataaacgtc agtatgttga cgaccgcgtg
     1981 atgagttaat cgttttttct tcggcacctt cttcgggagg tgccgtctgg ttaaacacga
     2041 tcccgctcgc atttttccct aagttaaatg agtaatctga tggtgtgtat ttcagataca
     2101 ccgtgtcagc cactaacagg gagtgcgtat gtttccagaa taccgagatt taatatcccg
     2161 tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat aaacacaata aacttgatca
     2221 tgaaattgcc agaaaggaag gttccgacgg tcgagggtac aatgcggaag tggtccgcat
     2281 gaaaaaacaa aagctacagt taaaagatga gatgctcaaa atcctgcagc aggagagcgt
     2341 caaagaggtg taaactttcc taagccgcca gccaggcggc ttttttaaca acagcatgga
     2401 ttgactggag ataagatgac tgaaacgata aaagtaagcg aatcacttga attacatgct
     2461 gttgcggaaa gtcacgtcac acctctttat cagttaatct gtaaaaataa aacctagtta
     2521 cagcagtcgc taaactggcc gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg
     2581 cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa
     2641 gatgaactta tcggcgttat ctcgtttaat cgtattgaac cactgaataa aactgctgaa
     2701 atcggctact ggctggacga atctcatcag gggcagggga tcatttctca ggcgctgcag
     2761 gcattgattc atcattacgc tcagtctggt gaacttagac gcttcgtgat caaatgtcgg
     2821 gtggacaatc cgcaaagcaa ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc
     2881 ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt
     2941 atcgattcat aataaccctg ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac
     3001 ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt
     3061 taactccaca tggttttcaa ttatcaccgc acctgtaata cggctctcac cttgtattac
     3121 aacgtgctcg tcgagcagaa tcggtccacc acgtaccacg gcatttccgc caatcaagac
     3181 atgatgtttt aacacacaat taccttccac aatggcatat tccgccacct gcgaactgta
     3241 atgaatcgtc ggaatggcat cttcttctat gccagctttc acctgcgcgt gaccgtagac
     3301 tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta ccttcaacac tggaaaaatc
     3361 aaaaacttcg gcgcgatgtt caataaaagc ataccggacg acggcatcgc cataaatttg
     3421 tgcctggtgg acaatacgcg aggcgctaac ccttgcgcga tcataaattt ggagcaggag
     3481 ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata gaatgttgat caattaaggc
     3541 gtgaccaaat attctacatt gcccgtaaac taatgagtca tgaatggtga cactgtcact
     3601 tatatatgca ccctggctga tttcactatt atcgatccag acgttatccg ttgcgtaaac
     3661 ctctccccac agcacgctgg tgccggtgat acgcgtatta ccggaaatca ctgttccgcc
     3721 gaacgcaata gcattctggt cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc
     3781 gcgatcgagc cagccgccag cggttcctgc tatcacatcg ttaaaatcgc taatggcgat
     3841 aatctgccgt aataacacac tttttttagt gccatcttcc tgataactaa aggctcgctg
     3901 ttcttcactt aggcgatatt tgcgcatagc gttttcccac aggtgactta ctataaccgt
     3961 agcaaattct gcggctctgg ctatgctcga gaaaattcca taaaatgcat ttcaaatata
     4021 ctttataaat taaacaaaat gagtaagaag atgcagagcg ataaagtgct caatttgccg
     4081 gcaggctact ttggtattgt gttggggacg atagggatgg gatttgcctg gcgctatgcc
     4141 agccaggttt ggcaggtcag ccactggtta ggggatgggc tggtgattct ggcgatgatc
     4201 atctggggat tattgactag cgcatttatt acccgactca tacgctttcc gcatagcgtg
     4261 ctggcggaag ttcgccatcc agtgctgagc agttttgtga gtttgtttcc ggcaacgacg
     4321 atgctggtgg cgattggttt tgttccgtgg tttcgcccac tggcggtgtg catgttcagt
     4381 tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga
     4441 tctcaccctg aagaagctac cacgcctgga ctgtatctgc cgacagttgc caacaacttt
     4501 atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg ccggtctggt gtttttaggc
     4561 gcaggcgttt tctcatggct aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg
     4621 ggagaattac ccacggcact gcggacatca ctcggcattc agctcgctcc tgcgctggtg
     4681 gcctgtagtg cctggctgag cgtcaacggc ggcgagggtg acacgctggc gaaaatgctt
     4741 ttcggttatg gactgctgca actgctgttt atgctacgtc tgatgccatg gtatctctcc
     4801 cagccattta atgcttcatt ctggagtttc tcgttcggcg tatctgcact ggcaaccacc
     4861 ggtttgcatc tggggagtgg tagcgataat ggatttttcc atacgctggc ggtgccgctg
     4921 tttatcttta ccaattttat tattgcaata ctgctcatcc gtacttttgc gcttctgatg
     4981 cagggaaaat tgttagtcag aaccgagcgc gccgttttaa tgaaagcaga ggacaaagaa
     5041 tgatcattcg tgacgaaaac tattttactg ataaatatga attaacccgc acacactctg
     5101 aagtactgga agcggtgaaa gtggttaaac cgggtaaaac gctggatctg ggctgtggca
     5161 atggtcgtaa cagtctttac ctggcagcca atggttatga tgttgacgca tgggataaaa
     5221 atgccatgag tatcgccaac gtcgagcgca ttaaatccat tgaaaatctg gataatttac
     5281 acacccgggt cgttgatctg aataacctca catttgatgg acagtacgat tttattcttt
     5341 cgactgtggt gctgatgttc cttgaggcta aaaccatccc agggttgatt gccaatatgc
     5401 aacgttgcac taaacctggt ggttacaacc tgattgtggc ggcgatggat accgctgatt
     5461 atccatgtac cgtcggcttc acgtttgcct tcaaagaggg agaattacgt cgatattacg
     5521 aaggctggga gagggtgaaa tacaatgaag acgtcggcga gctgcaccgc accgacgcca
     5581 acggtaatcg tattaaactg cgtttcgcca cgatgctggc acgtaaaaaa tgacccggta
     5641 agcacaaaac gcgtgaaaat tccccacgct gagatgattt actgttcttc ttttcggtaa
     5701 gcatattttt tatcgaaggg atgtgaaatt aatcacagta gtcgaagttt tttagcagct
     5761 taacttactg aaatttaact actgatgatt gacttagccc ctttttcggc attgactatg
     5821 tcgtctgaaa aggggctgaa aaatttattt tcaccaacac tttttttgcc acaacacgaa
     5881 gcggcgcttt ttgctataac ttagaaagta atataatcat ctcaggaaac tattcatgcg
     5941 taccacatca tttgcgaaag ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc
     6001 ctctaaaatc acccagccag ataaatattc tggtttttta aacaattact ctgatttaaa
     6061 agaaacaacc tcagcaacag gtaaacctgt tttacgttgg gtagacccga gttttgatca
     6121 aagcaaatat gacagcatcg tctggaaccc aatcacttat tatccggcac caaaaccttc
     6181 tacccaggta gggcagaaag ttctggataa aattttgaac tataccaaca ccgaaatgaa
     6241 agaagcgata gcgcagcgta aaccagtggt taccaccgct gggccgcgta gtctgatttt
     6301 ccgtggggcc attaccggtg tagataccag caaagaaggg ctgcaattct atgaagtggt
     6361 tcctgttgca ttagtggttg cggggacgca aatggctaca ggccaccgta ccatggatac
     6421 tcgcctctat tttgaaggtg agctgattga tgcagcgact aataaaccgg ttatcaaagt
     6481 cgttcgtcag ggcgaaggta aagacctgaa taacgaaagt acgccaatgg ctttcgaaaa
     6541 tattaaacaa gttattgatg acatggcgac cgatgccacc atgtttgacg ttaacaaaaa
     6601 gtagttccag acgcgccatc gttagatggc gctttttatc cggtgcgccg taaaacccca
     6661 tccttcaggg cggggatata aggcgcggtt ttccacctga ccaggtgttt gctgt
//
LOCUS       DADKXM010000170         6625 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.173, whole genome shotgun sequence.
ACCESSION   DADKXM010000170 DADKXM010000000
VERSION     DADKXM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.173"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..233
                     /locus_tag="JQR05_003598"
     CDS             <1..233
                     /locus_tag="JQR05_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325636.1"
                     /translation="IGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALK
                     MPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            322..1782
                     /locus_tag="JQR05_003599"
     CDS             322..1782
                     /locus_tag="JQR05_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY5325637.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKTAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1818..2021)
                     /gene="ydfZ"
                     /locus_tag="JQR05_003600"
     CDS             complement(1818..2021)
                     /gene="ydfZ"
                     /locus_tag="JQR05_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY5325638.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2199..2885)
                     /locus_tag="JQR05_003601"
     CDS             complement(2199..2885)
                     /locus_tag="JQR05_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5325639.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2974..3720)
                     /gene="ydfG"
                     /locus_tag="JQR05_003602"
     CDS             complement(2974..3720)
                     /gene="ydfG"
                     /locus_tag="JQR05_003602"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY5325640.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3857..5902
                     /gene="dcp"
                     /locus_tag="JQR05_003603"
     CDS             3857..5902
                     /gene="dcp"
                     /locus_tag="JQR05_003603"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY5325641.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5947..6465)
                     /locus_tag="JQR05_003604"
     CDS             complement(5947..6465)
                     /locus_tag="JQR05_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY5325642.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1650 a         1578 c         1678 g         1719 t
ORIGIN      
        1 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
       61 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      121 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      181 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
      241 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
      301 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
      361 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
      421 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
      481 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
      541 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
      601 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
      661 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
      721 gcgaccggac agttaatgct cgatcacccg atggtcgctg ccgacgtgca aaatccccac
      781 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa aacggcagga
      841 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
      901 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
      961 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1021 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1081 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1141 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1201 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     1261 tgtatggaag atgaacatta tcgtcatgcg gcgtatggct tgatgttgca ggaacaagcg
     1321 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     1381 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     1441 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     1501 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     1561 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     1621 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     1681 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     1741 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     1801 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     1861 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     1921 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     1981 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2041 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2101 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2161 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     2221 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     2281 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     2341 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     2401 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     2461 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     2521 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     2581 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     2641 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     2701 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     2761 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     2821 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     2881 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     2941 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3001 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3061 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3121 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3181 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     3241 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     3301 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     3361 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     3421 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     3481 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     3541 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtcttt
     3601 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     3661 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     3721 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     3781 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     3841 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     3901 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     3961 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4021 caacaatact attctggcac tggaacaaag cggagaatta cttacccgcg ttaccagcgt
     4081 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4141 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4201 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     4261 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     4321 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     4381 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     4441 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     4501 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     4561 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     4621 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     4681 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     4741 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     4801 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     4861 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     4921 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     4981 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5041 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5101 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5161 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     5221 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     5281 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     5341 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     5401 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     5461 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     5521 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     5581 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     5641 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     5701 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     5761 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     5821 tctggaacgc ctgcatcgac aatggcgcgg taaggctcct cagattatgc cgatgctgca
     5881 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     5941 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6001 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6061 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6121 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6181 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     6241 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     6301 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     6361 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     6421 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     6481 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     6541 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     6601 tgagtcaaat tccctgaata tttga
//
LOCUS       DADKXM010000171         6606 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.174, whole genome shotgun sequence.
ACCESSION   DADKXM010000171 DADKXM010000000
VERSION     DADKXM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.174"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..756
                     /locus_tag="JQR05_003605"
     CDS             <1..756
                     /locus_tag="JQR05_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY5325643.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFD
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="JQR05_003606"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="JQR05_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY5325644.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSHSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQMGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="JQR05_003607"
     CDS             complement(1792..1995)
                     /locus_tag="JQR05_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY5325645.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="JQR05_003608"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="JQR05_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY5325646.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="JQR05_003609"
     CDS             complement(4588..5100)
                     /locus_tag="JQR05_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY5325647.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..>6606)
                     /gene="hpaB"
                     /locus_tag="JQR05_003610"
     CDS             complement(5118..>6606)
                     /gene="hpaB"
                     /locus_tag="JQR05_003610"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY5325648.1"
                     /translation="TNGSVRYCQYTSDVTTHPAFRNAAASVAQLYDALHKPEMQDSLC
                     WNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGA
                     NPGFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHLPTDKVKDVYIKLEKETDAG
                     IIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRASYEM
                     VAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMY
                     PLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSE
                     ATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSGLIYLPSSARDLNNPQID
                     QYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQ
                     AQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK"
BASE COUNT         1622 a         1713 c         1728 g         1543 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tcagctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaagcaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt tgatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccatctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agcgctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagtgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg atcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcgatcgg tgggttaaca atcgcgtggt taaagtagag gccagtttcc
     6241 tgaatacggg tataccagtt acgggcgttc tgctcgaact gaccgtaaaa gcccggattc
     6301 gcgcccagtg cgcaaccgaa agcggctttg tagtctgggg tacggcccat ccagccatag
     6361 ctcaggcgtg accattcagc gatggcgtcg cgttgctggc gcaggtcgtc ggcacttttc
     6421 gccacgcgga agaatttatg ggtatagccg ccgctgccgg tgtcggtgtt ccaacacaga
     6481 gagtcctgca tctccggttt gtgcagcgcg tcgtacagtt gggcaacaga cgctgccgca
     6541 ttacgaaatg ccggatgagt ggtgacgtct gaggtgtact ggcaatagcg gacactacca
     6601 tttgtt
//
LOCUS       DADKXM010000172         6564 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.175, whole genome shotgun sequence.
ACCESSION   DADKXM010000172 DADKXM010000000
VERSION     DADKXM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.175"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..319)
                     /locus_tag="JQR05_003611"
     CDS             complement(<1..319)
                     /locus_tag="JQR05_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325649.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMT"
     gene            complement(577..768)
                     /gene="yaiA"
                     /locus_tag="JQR05_003612"
     CDS             complement(577..768)
                     /gene="yaiA"
                     /locus_tag="JQR05_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY5325650.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(818..1342)
                     /gene="aroL"
                     /locus_tag="JQR05_003613"
     CDS             complement(818..1342)
                     /gene="aroL"
                     /locus_tag="JQR05_003613"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY5325651.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(1525..1983)
                     /locus_tag="JQR05_003614"
     CDS             complement(1525..1983)
                     /locus_tag="JQR05_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY5325652.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2103..2912
                     /gene="proC"
                     /locus_tag="JQR05_003615"
     CDS             2103..2912
                     /gene="proC"
                     /locus_tag="JQR05_003615"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY5325653.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2929..4044)
                     /gene="adrA"
                     /locus_tag="JQR05_003616"
                     /gene_synonym="dgcC"
     CDS             complement(2929..4044)
                     /gene="adrA"
                     /locus_tag="JQR05_003616"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY5325654.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4146..4466)
                     /gene="psiF"
                     /locus_tag="JQR05_003617"
     CDS             complement(4146..4466)
                     /gene="psiF"
                     /locus_tag="JQR05_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY5325655.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4585..6000)
                     /gene="phoA"
                     /locus_tag="JQR05_003618"
                     /pseudo
     CDS             complement(4585..6000)
                     /gene="phoA"
                     /locus_tag="JQR05_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(6101..6361)
                     /gene="iraP"
                     /locus_tag="JQR05_003619"
     CDS             complement(6101..6361)
                     /gene="iraP"
                     /locus_tag="JQR05_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY5325656.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1586 a         1736 c         1547 g         1695 t
ORIGIN      
        1 cagtcatact actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat
       61 cgagcacttc aaccacacct tgcagatctc gctccacttt acgacgcgaa acatgggcca
      121 gctggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg
      181 ccatcacttc ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt
      241 caatgtattc cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg atggtcagga
      301 tcgccaacga cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg
      361 caagtgtgaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag
      421 cagacgtgca cggcataatg taaagattcc aaatgattcc agtaatggaa tgttattgtt
      481 taatattcta attattagaa aaacatgaat tatgaaaaat gtgacgcaga tcccttgtag
      541 tggcactcag aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata
      601 gcgttttttc gcatccatcg cttcctgagc cgtcggatgt tcactgatca acgagtctgg
      661 tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttttc
      721 aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta
      781 tttcacggga tgaacgttaa gtataggcgc tctaaaatca acaattgatc gtctgtgcca
      841 gggcgctacg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat
      901 gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca
      961 gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa
     1021 ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac
     1081 gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat ggcgcagtta
     1141 ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga
     1201 tctccgcgac cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac
     1261 ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga
     1321 tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc
     1381 cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa
     1441 aattccactt aaagtgaaaa tctcaataca tcccttacta taccaataaa tattcaagaa
     1501 tgaagtgtaa ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt
     1561 ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt
     1621 ctggatccca ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat
     1681 ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc gctgcgcctt tctcgatggc
     1741 ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac
     1801 aatttcgtta tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga
     1861 tggcggcacg cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc
     1921 cgcgcgatac aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt
     1981 cataaagtca tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata
     2041 gttctgttta tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg
     2101 caatggaaaa gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg
     2161 gtctgattgc cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccgg
     2221 ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag
     2281 tggcgcaaat cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc
     2341 ttagcgaaat cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg
     2401 tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc
     2461 cgaacactcc cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa
     2521 ccccagaaga taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa
     2581 ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtat
     2641 ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg
     2701 cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag
     2761 aacatccggg ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg
     2821 tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta
     2881 tggaaaaatc agaaaaactc agcaaatcct gatgactttc gccggacgtc aggccgccac
     2941 ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa
     3001 ccactcacga tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa
     3061 agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat
     3121 ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc
     3181 aaaccgacca atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac
     3241 aatcgcttca tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat
     3301 atcgataatc agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg
     3361 taacatagtt tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gagtactcat
     3421 gacctgcaac ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc
     3481 aaacagcaga ggataaacga caataatggg aagggagagc caccattcca gcggcgcact
     3541 attgaacgac acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa
     3601 caccagaccc gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat
     3661 catcaacata gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc
     3721 taatactgca tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct
     3781 cgctatctgc caagctaaat gcggccagac gaacgcccag ccgaccaaca ccagccacca
     3841 ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag
     3901 gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc
     3961 gttttgagga gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc
     4021 atcattcatt atttttggga acattcagaa acaattttcc caaattatag agacggggct
     4081 aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta gctcacccgt tagcactatc
     4141 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
     4201 agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
     4261 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
     4321 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
     4381 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
     4441 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
     4501 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
     4561 gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca tggtgtagaa
     4621 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
     4681 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
     4741 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
     4801 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
     4861 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
     4921 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
     4981 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
     5041 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
     5101 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     5161 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     5221 gttcaccaac tgataaccac gcgcctgtgc ctattcacgc agcgtttttc cctgccattc
     5281 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     5341 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     5401 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     5461 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     5521 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     5581 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtgac
     5641 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     5701 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     5761 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     5821 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     5881 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     5941 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     6001 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     6061 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcttca
     6121 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     6181 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     6241 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     6301 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     6361 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     6421 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     6481 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     6541 tatcaacctt tattataaat aaaa
//
LOCUS       DADKXM010000173         6488 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.176, whole genome shotgun sequence.
ACCESSION   DADKXM010000173 DADKXM010000000
VERSION     DADKXM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.176"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JQR05_003620"
     CDS             complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JQR05_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY5325657.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPLCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1522..1848)
                     /locus_tag="JQR05_003621"
     CDS             complement(1522..1848)
                     /locus_tag="JQR05_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY5325658.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1983..2324
                     /locus_tag="JQR05_003622"
     CDS             1983..2324
                     /locus_tag="JQR05_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY5325659.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2390..2919
                     /gene="speG"
                     /locus_tag="JQR05_003623"
                     /pseudo
     CDS             2390..2919
                     /gene="speG"
                     /locus_tag="JQR05_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2922..3632)
                     /locus_tag="JQR05_003624"
     CDS             complement(2922..3632)
                     /locus_tag="JQR05_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY5325660.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3740..4045
                     /locus_tag="JQR05_003625"
     CDS             3740..4045
                     /locus_tag="JQR05_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY5325661.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4244..5701
                     /locus_tag="JQR05_003626"
     CDS             4244..5701
                     /locus_tag="JQR05_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY5325662.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWLETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     LRDGVRGKDKLDVPIKFIWNFKDLKRTLSRPEVLH"
BASE COUNT         1727 a         1655 c         1517 g         1589 t
ORIGIN      
        1 cttcgggata acgcttaccg ctcatgggca cctctcttta agccatctta aatgactctg
       61 aggtgtctgt taaacccgtg gcgattcaat tatcttactc cttacctgtt ccacagcgtg
      121 ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc
      181 ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata gccgttatcg
      241 aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtattcctgg
      301 acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca gagtggtgac
      361 aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc
      421 cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt
      481 tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg
      541 ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc tggcgtcggg
      601 tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata
      661 ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt
      721 accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct
      781 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
      841 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      901 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      961 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
     1021 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
     1081 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
     1141 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
     1201 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
     1261 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
     1321 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcca ctgccataca
     1381 agtatgaaga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga
     1441 atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct cgtatcttat
     1501 tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacattcc
     1561 gcaaagcgca atcaacgcac ccgtccagtc ataaagactt agtttcacgc catccacaac
     1621 gcgcagccac atcaatgccg tgcagacata aacgccacca taagccgcgt aaacacgccc
     1681 actcgccgca ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg
     1741 aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat
     1801 aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta
     1861 ttgacatcat gtatagttat agggcgacat aatatcatca atataaacat cctcctggta
     1921 cgttttgcgt tcgcagtgga tggtgtagaa tcacctttaa tcattcatac agggaatgaa
     1981 ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac
     2041 tggcattgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca
     2101 gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc
     2161 tgcgccagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg
     2221 ataaccgcga taaatgtgag caaagcgcca acatcaatgc ctactgggag cccaatactt
     2281 tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa
     2341 ggaatgtaag gacacgttat gccaagcgcc cacagttaag ctacgcccgc tggagcgtga
     2401 agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga
     2461 ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcgca tattcacgat
     2521 cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg
     2581 gtggaaatta accatgttca tcgccgggca gaatttcaga taattatctc cccggagtat
     2641 caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt
     2701 ctcaatctct ataagctgta tctgatcgtc gataaagaga atgaaaaagc gattcacatt
     2761 taccgcaagc ttggctttac ggttgaaggt gaattgatgc atgagttttt tattaatggt
     2821 caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa
     2881 acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta
     2941 tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgatcagc
     3001 tgacagtcca ccggattggc gtgactgtca tattcacagc tctgttttac attaccaact
     3061 cgttgattat ttagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat
     3121 ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggatac
     3181 ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag
     3241 ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt
     3301 ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt
     3361 accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct
     3421 tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc
     3481 tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg
     3541 gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc
     3601 gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc
     3661 tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc
     3721 ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct
     3781 cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga gcgatatatc
     3841 acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa
     3901 tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgcgctaatg atacgcataa
     3961 aattctctac aaccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga
     4021 tggcgcgcct gccgaaccgc agtaacacat tatcgactga acgccggata tgacacatcc
     4081 ggcgatttga acatacaaca taatctcacc ttattactca tacccttcta ttgatgtgga
     4141 ttaataattc ttaacccaaa atgggtaggc tccctctatt gttagcgcgc taaatattca
     4201 atatataaac atttatataa cgataaagaa cagggagtga gttatgtcca aaaatgaccg
     4261 aatggtgggc atcagccgca gaacactcgt taaatcaacc gcaataggtt ctctggcgct
     4321 ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcag cggtacaaca
     4381 ggccagcgaa aaagtgatct ggggttcctg ttccgtcaac tgtggtagcc gctgtgcact
     4441 tcgtctacat gttaaagata atgaagtgac ctggttggaa actgacaata ccggcagcga
     4501 tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat
     4561 taatcatccc gatcgcttga attacccaat gaaacgcgtg gggaaacgtg gtgaaggcaa
     4621 attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc tgaaaaaaac
     4681 cgtcgaacaa tatggcaatg aagctgtata tattcagtac tcttcgggga ttgttggcgg
     4741 caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga actgctacgg
     4801 cggttcactc aatcagtatg gctcctacag caccgcgcaa atttcctgtg ccatgcccta
     4861 cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca agctggtcgt
     4921 gatgtttggt aacaacccgg cagaaacccg catgagcggt ggtggcatca cttatcttct
     4981 tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt atcgatccgc gatataccga
     5041 tacggctgcc ggtcgtgaag acgaatggct ccctattcgc ccgggcaccg atgccgcgct
     5101 ggttgcgggt attgcctggg tattgattaa cgaaaatctc gttgatcaac cttttctcga
     5161 taaatactgc gtcggctatg acgaaaaaac cttacccgca gatgcaccca aaaatggtca
     5221 ctataaagcc tatattcttg gtgaaggtga cgataaaaca gcgaaaacgc cgcagtgggc
     5281 ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa ctggcgcgtg aaattggcac
     5341 aacaaaaccc gcttatatct gccagggctg ggggccgcaa cgccaggcaa acggcgagct
     5401 gactgcacgc gctattgcta tgttacctat tttgacgggc aatgtcggca tcagcggcgg
     5461 aaatagtggc gcgcgtgaat cgacctatac cattacgata gaacgcctac cggtgctgga
     5521 taatccggtc aaaacgtcaa tctcctgctt cagctggacg gatgctatcg atcatggccc
     5581 gcaaatgacg gcactccgcg acggtgtccg gggcaaagat aaactggatg tgcccattaa
     5641 gttcatctgg aacttcaagg acttgaagcg gacccttagt aggcctgagg tcctgcattg
     5701 agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc attagacggg atgtctagac
     5761 ggctggtcag gacccccggt ggcctcggtc tggacttgag gtgggcctat ggggggaagt
     5821 ctgctggctt aatgtattag atggtcattc acatttttct gtcagaaacc atcggtccca
     5881 ctgtgagtcc aaacctaagg acctagaagt ccacccacag ccttagacag agtgtcgaac
     5941 acatccacac cgagtcgacc agagaggacc atgtagagac ccacagagag gaacacgagt
     6001 ttatagacca gagtcaggag ggacttcagg tctgcggaag tcatagacat aaagccccta
     6061 tctgttatgt tatattatta cattaacaac agatccagac cattggtcca gaccatagtt
     6121 agttaatcac agtgatagcc caccacctga agtcctgctg atattcagtc cagacttacc
     6181 tctgctcatg gtcttacctc cctcggtctc ctgaccttgg gtatcgacta cagcttgctg
     6241 agcgtcatct ggaggagcta caggaggcgg gaccgaagcc ccaccttgag tcccaccacc
     6301 acctgtcagg cccttggcga tggacctgaa gcctttacta atgctcttac ccattggtct
     6361 tacgcttcgg tcttggtgaa cttcaggcgt ttgactacag gcaggacggc agtagctgag
     6421 ccagtgccca tcttgacggt cgtagcagct tcagcgacaa cgaacacttt accgaggatt
     6481 tccaagtc
//
LOCUS       DADKXM010000174         6471 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.177, whole genome shotgun sequence.
ACCESSION   DADKXM010000174 DADKXM010000000
VERSION     DADKXM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.177"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JQR05_003627"
     CDS             complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JQR05_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY5325663.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQ"
     gene            complement(928..1023)
                     /locus_tag="JQR05_003628"
                     /pseudo
     CDS             complement(928..1023)
                     /locus_tag="JQR05_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="JQR05_003629"
     CDS             complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="JQR05_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY5325664.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="JQR05_003630"
     CDS             complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="JQR05_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY5325665.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2120..3212)
                     /locus_tag="JQR05_003631"
                     /pseudo
     CDS             complement(2120..3212)
                     /locus_tag="JQR05_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3214..3789
                     /locus_tag="JQR05_003632"
     CDS             3214..3789
                     /locus_tag="JQR05_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5325666.1"
                     /translation="MALNGGDTFTIKDVKVMPESIPAGYEVLQELDELDSLLIIDLGG
                     TTLDISQVMGKLSGISKIYGDSSLDVSLVTSAVKDTLSLARTKGSSYLADDIIIHKKD
                     NNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGGGAELICDT
                     VKKNTQIRNERFFKTNNSQYDLVNGMYLIGN"
     gene            3792..4143
                     /locus_tag="JQR05_003633"
                     /pseudo
     CDS             3792..4143
                     /locus_tag="JQR05_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
     gene            complement(4293..4466)
                     /locus_tag="JQR05_003634"
     CDS             complement(4293..4466)
                     /locus_tag="JQR05_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325667.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQTLRTHQF"
     gene            complement(4519..4794)
                     /locus_tag="JQR05_003635"
     CDS             complement(4519..4794)
                     /locus_tag="JQR05_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY5325668.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(4794..5078)
                     /locus_tag="JQR05_003636"
     CDS             complement(4794..5078)
                     /locus_tag="JQR05_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY5325669.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            5530..5781
                     /locus_tag="JQR05_003637"
     CDS             5530..5781
                     /locus_tag="JQR05_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325670.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            5750..6040
                     /locus_tag="JQR05_003638"
                     /pseudo
     CDS             5750..6040
                     /locus_tag="JQR05_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(6140..6325)
                     /locus_tag="JQR05_003639"
                     /pseudo
     CDS             complement(6140..6325)
                     /locus_tag="JQR05_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1698 a         1502 c         1517 g         1754 t
ORIGIN      
        1 ttgcagagca tgggatgata atgggttatc actaatatgt attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttcta tcaaaaaaat actctctgac
      121 aacgggatta tttccctcag aaaatgctgg tagctcagtg aggaaattac gggtcgctct
      181 gagattttta agagcctgtg ggagcgatgg taaggagact atttcgttat agctgatatt
      241 catagtcaat aacgaatcgg gtaaagctgg caggttttcc agctcattgt gttgtacctg
      301 aagtgactcc agcgcaggag gtagcacggg caattctgat aatttattgc tggcggaata
      361 taattttttt agcgtaacag gcagttcagg taggttagtt aattgattat atgatacatt
      421 gagcagcgtt atctgagctg gtaagttgtc aggtagcgag gacagattta aacggtccag
      481 tcgaagttca tcggaattat tgataagaca ttctttaagt cgggagacag cctcatcacg
      541 ctcttcaccg gggagcgctt gtttttccca tttatcccat gctgaaaagt aatcagcata
      601 tgtaccggaa atagtgttat aaaaagaatt ttggggcaat gaaaagttat tatttatcgg
      661 taacatgatg ggcatccagt taaagccggt ggacaaatgg ctttgttaaa agaaataagt
      721 aggagttttt atgaagttca ttccatgaaa tgaacttcag cactttttta ttataataag
      781 gataaacaaa attggctgta aaaaaacgat actgtttcag ttaaccgtga atggcctgtt
      841 tgcaccgtat tgctcttgtt tttcggttag ttaaacatcg tacttcatat ttgaacggta
      901 agcggctcgt cagaaccgta ttgatattta ctgagaggtc agatcaactt tccagggcaa
      961 cagatcgcgt acccggtttg ccggccagtc ctggatatgc tcaatgacgt aacgcagcca
     1021 ttttgtcaac gccacgattt ttgaccctag cctgacattt atattcccca gaacatcagg
     1081 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     1141 acgaagatcg gcacactggc catatcagtg gtcatcatgc gccagctttc atccccgaca
     1201 tgcaccaccg ggtaaagctc acgggagact ttatctgaca gcaggcgggc actggccagg
     1261 gggatcacca tccgtcgtcc gggcgtgtca ataatgtcac tctgtacatc cacaaacaga
     1321 cgataacgac tctctctttt ataggcgtaa accttaaact gcatttcacc agtccctgtt
     1381 ctcatcggca aaagagccgt tcatttcaat aaaacgggcg atctcagcca tcccctgctg
     1441 attttcagcc tgccagcgtt ccgcacgcag acggcgggcc tctttttgca tggccgtatt
     1501 taccaggcct gagatattca cgtttgcgga tttaagtaac tgatagctgt cactgtctat
     1561 agtgacagta atacgctgct tcatgataag ccctcaaaaa catacttatg atatgtatat
     1621 gagtatatat taatggtaca aaccatcaat atccataaaa agtacaccat taattcgcta
     1681 taagttttta atacaatttt ttctgctgcg atttttttat acgataatta gcatacttat
     1741 aacaccgtaa gtaaattaca tgaaagcagg cacagcgtga tccgattttt ctgcgtataa
     1801 cgattttccg gaagtgtaat acggttgcgc cccgggccgg gcaccctttt ctgttgttgt
     1861 gtacgggccc acagagaatg gtaccggagg cgggctcaga agtactgagt cgggtgatcg
     1921 cgtcaggccg cgaagtactg acacgggtat tggtgactgg tgatgaagta ctgccccggg
     1981 tatgatttca ggctgattca ggttctgatt ctctgtcagt ccgcgtggtt gctggcctag
     2041 ctgatttcct gttgtcaggc gttcaacaac cgactgcagg ttatagcccg gttcacgaac
     2101 acctgacggc aggtatcact cacccggtga aggtaaatcc ctcaagaggc agcaactctg
     2161 ccagtcgctc ctccggccat tccggcagac gcgtcaggac gtccgtcagc caggcatgtg
     2221 gctccagacc attacgtttt gctgtttcca gaaggctcat tatctgtgcc gcccggtttc
     2281 cggccatcag tgaaccggcg aacagccacg ctttccgtcc cataacaacc gggcgaatgg
     2341 cacgctcgca gatattgttg tccaggggca ccgctccgtc acccaggaag cagctcagtt
     2401 catcgcgccg gttcagaata tagttaatcg ccttatttaa tggcccgtcg ggcggacagc
     2461 acggctcctg ctcttcaagc caggcaaaca ggtcattcac tatcggccgg gagtaccgtt
     2521 atcgccactg ccgtattttt tccacagggc gctcacggat gagtttctca atgcggtaaa
     2581 gacccgcgat ttttctcagc tccacgcctg cacggggctc cctgctggcc ttatacagat
     2641 ttgcgaagcc gcgccctgcg tgactccagc aacaggctga tgtgatgccg gggtgattat
     2701 ccgccagaga tttgtacacg ctgtatccgt cactcaccag cgtaccgcca caccagcact
     2761 gcagccaggt ctcagggtat ctgagggccc gtccggtctg cgagtcaaaa cagacgaccg
     2821 gcggaccgct tctttcaccg ctgacgtaag cacacagcca gccggagcag gatttaccgc
     2881 ctttccgggt atcaaggagc ctcagggacg tttcatctgc atgaataaca tcccgtgtca
     2941 gaagctcatg gcgtagcagt ttcgccagcg gggccagtgc agctccggca acaccgacca
     3001 tatccgccat tgtactgacg gggagctcca cacccattcg ggagaaaatg tgctgctggc
     3061 gatacagggg cagatggtcg gtatatttgc tgatgacaac ctgtgcaatt accgacgact
     3121 caacaagcac tttcagggag gatatgcgcc ggcattttcc cgctgaacac tttctcacag
     3181 cagggacagc tgtactgcgg acggatatag cggattgcat taaatggtgg ggatacattc
     3241 acaataaaag atgtaaaagt catgcctgaa tctataccgg caggttatga agttctacaa
     3301 gaactggatg agttagattc tttattaatt atagatctcg ggggcaccac attagatatt
     3361 tctcaggtaa tggggaaatt atcagggatc agtaaaatat acggagactc atctcttgat
     3421 gtatctctgg tcacatctgc agtaaaagat accctttctc ttgcgagaac aaaaggaagt
     3481 agctatcttg ctgacgatat aattattcac aaaaaagata acaactatct gaagcaacga
     3541 attaatgatg agaacaaaat atcaatagtc accgaagcaa tgaatgaagc acttcgtaaa
     3601 cttgagcaac gtgtattaaa tacgctcaat gaattttctg gttatacgca tgttatggtt
     3661 ataggcggtg gcgcagaatt aatatgcgat acagtaaaaa aaaacacaca gattcgtaat
     3721 gaacgttttt tcaaaaccaa taactctcaa tatgatttag ttaacggtat gtatctcata
     3781 ggtaattaat aatggacaag cgcagaacca ttgccttcaa actaaatcca gatgtaaatc
     3841 aaacagataa aattgtttgt gatacactgg acagtatccc gcaaggggaa cgcagccgcc
     3901 ttaaccgggc cgcactgacg gcagatctgg ccttatacag acaagatccc cggcccccca
     3961 tttcttttat gtgaactgct gacgaaagaa accacatttt cggatatcgt gaatatattg
     4021 agatcgctat ttccaaaaga gatggtcgat tttaattctt caacaatcac tcaatcctct
     4081 tcacaaaaag agcaaaaaag tgatgaagag acccaaaaga acgtgatgaa gctaataaac
     4141 taattcaatt attattgagt ttcctttatc cactatcagg ctggataaag gaaacttaat
     4201 caagttattt tcttgccaat cattacataa aataaacagg gtcgttatta tgaaattaat
     4261 ccacggtgct tatgaaaaag cggctccaat aatcaaaatt ggtgagttcg caatgtctgg
     4321 caataacgtt tctgatgggt tatttgccag cccggagcgc gatattgccg ccagtcacga
     4381 gcggtaattt tcgttatttc atcgtaattt attttatctc ctacaagatt atttaatgga
     4441 atgcaaaata tctggcctga taacaatgct ccagaattaa tacctgcagg ctatgttaaa
     4501 gttgcctcca cacccaatct acctgttctc tcgtgtgtgc cacaggcgca atacataaat
     4561 agtctggcca gtaagttcgt aacggatttc gtactcgcca gtaaaaatcc gtctgacctc
     4621 cctgggttca aactgaaaca actgttctcc catacgtgga tgagttaaca gaatgaccgg
     4681 cgcctgtgtc agggactgca ccgttctggc ggcagcaggt ttactggcca gcaccagaaa
     4741 atcatataac cgcgccaaat cagaaagtgc cttactggtc cacttcagtt ccatcagcgt
     4801 ggcaccggta acggatggtc agtactgagg ctgtccgccc aggcctgtac agcctgatgg
     4861 tcgataacct gtccggatgt cacatcgtcc agggcttcca gcgtcaggcg attacgctcc
     4921 tcctcttggg caagccatgc agaaagcgcc tgtttgataa cccagccccg ggagcgttcc
     4981 agtctggcgg ccatctggtc gactttatca gccataggta atggcacatg cgcggtaatt
     5041 acttttgttg cttgtgcggt attgtttttc atttgcatac ccgattagtt gttaatcata
     5101 tttaatcata gtgattactt tcagacggaa caaggcaaat aatgcgagtc agcgcatata
     5161 aggcggcata cggtggttac atgtagcaca ggtgtaaaac cgccgaagcc cggcgcaagc
     5221 cggtacggtc gtccgaatat caccagtccc cctccggctc tgcgaggcca tgcggcctgt
     5281 cagcgatgaa aatgcctctg tgcgcggtcg gagtggtccc gacaagggtt tacccgcagt
     5341 cgaggcgtat ctccgcgtta gcgggccgtg agggccgctt acgagcgtgt atgagagacg
     5401 tacagtagtt ttgtccgctc acaccagtac aaaccagtcg aaataccggt aacgacgatt
     5461 actcttgtct ctgatgccgg tggactacga gattttattt actggcgtcc tgatgcggtc
     5521 aggactggta tggaacctgt ttatgtgata cttaatgctc ttctggattc cgggcgattt
     5581 acgcgtaaac ttattctgtt agggctgtca ggctcatttt cctatatctt cggctcaatt
     5641 gttgcgacac tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc
     5701 gtactgatac tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca
     5761 ctggagttcg aaaaagagta attttttcag gaaaaacata aaattactga ctaaatattt
     5821 attttttgaa tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta
     5881 tggatacagt atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt
     5941 tcagcttcat ttccggtaga aaaattatga cggcataatg gatgctgaaa ccgctgccat
     6001 cctgtatgca ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg
     6061 cgatatatcc ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg
     6121 tgatgtcaga tgtcccctgt aaaactatgt tttcattgcg ctctatgaag ctgagtgagc
     6181 gtaacgactg gagctgcttc tgcggtgctc cggtggcgaa cgtgaagtca tactcttcga
     6241 acattttcac cgccgggaag gctaccattc gggtatacat cgcctgttta cgttgatgac
     6301 gtgccagttt ttcttcatga agcatgatgc ttcaagaagg gcactgttgc aaagttatcg
     6361 atgaggtagc tgaccatcac tgctgtcgga atcgatcttg cccttctgtt tccagtcgcc
     6421 tcagcaaact atttttcagt taccacctgt cctgcggtaa gcgtaaaccg a
//
LOCUS       DADKXM010000175         6407 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.178, whole genome shotgun sequence.
ACCESSION   DADKXM010000175 DADKXM010000000
VERSION     DADKXM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.178"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JQR05_003640"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JQR05_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY5325671.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JQR05_003641"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JQR05_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY5325672.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5687)
                     /locus_tag="JQR05_003642"
                     /pseudo
     CDS             complement(2659..5687)
                     /locus_tag="JQR05_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(5712..5969)
                     /locus_tag="JQR05_003643"
                     /pseudo
     CDS             complement(5712..5969)
                     /locus_tag="JQR05_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
     gene            complement(6194..>6407)
                     /locus_tag="JQR05_003644"
     CDS             complement(6194..>6407)
                     /locus_tag="JQR05_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325673.1"
                     /translation="LSSILQHEDQEAFEEIFKTSCFMPKKGLKVDKVVSMGWTSGVAH
                     VKVYVKGNLYDLWTNTENLQSEKAGK"
BASE COUNT         1704 a         1485 c         1499 g         1719 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgtttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgatctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgta cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgccggg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt gagactgata aacgccaggc cgttgttttg cccctggccg
     3841 ctgaagccaa agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga
     3901 taataatccg tcacctgttg cagtactttc gtggtgttaa ccatggtggc accggaaggt
     3961 aactgcgcgg tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta
     4021 cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg
     4081 gtacaacgca acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc
     4141 gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc cttcctgcgc ggctttcaga
     4201 atggtggcgc acagggcagg ggtcaggctc attgccacaa atactgaaag cagcatggag
     4261 gagatcagcg tgatggagaa ctggcggtag atctccccgg ttgcaccgct cataaaggcc
     4321 atcggcataa acactgcaga aagaacaacg gcaatcccga ccagcgcacg ttggatctgc
     4381 cccatcgatt tatgcgtcgc ttccttcggc ggtagcttat cttccgcaat gacacgctcg
     4441 acgttctcca ccaccacgat ggcgtcatcc accagtaacc ctatcgccag caccatcccg
     4501 aacatagtca acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga
     4561 ataaccaccg gtacggcaat tgtcgggatg atggtggcac ggaaattctg caaaaacaga
     4621 tacatgacca ggaagacgag gatgatagcc tcaaccagtg ttttgaaaac ttcctgaata
     4681 gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa
     4741 taggctgata agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct
     4801 ccggcagcca gtttgatggc gatcccggca gcaggtttgc cgttatagcg cgccacggtg
     4861 gaataatctt ccgccccaag ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca
     4921 tcttgctgaa ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc
     4981 acaatgatcg aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca
     5041 ccagaaatct ggttgttttg caccttaatc tgggaaataa catcggaagg taccaggtta
     5101 tatttattga gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc
     5161 tgtacgctac caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag
     5221 tccgcgatat cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcagg
     5281 atattactgc tcgacttatc gacgctaatc ccctgttgct gcaccgcttc aggtaatgaa
     5341 ggcatagcga gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgtccca
     5401 gtctcgaagg tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac
     5461 atcaggccat caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc
     5521 gtttgcgcat cggcacccgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc
     5581 ggatactgcg caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg
     5641 gcaagtacct aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta
     5701 agcctgcaac gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct
     5761 cgtgctttga tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc
     5821 tgcaagccag aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt
     5881 agctgcacga catcgtcttt atccagaatg agcgccgttg ctttaccctg ggcgttgtgg
     5941 gtgacgcctt cctgcggcac cagtaatata aagcgcgcaa aattattcaa gatatttttg
     6001 aaaatcattc aagataggct atcgcgtgtg atagcaaaaa cattcaagat aaaaatgctt
     6061 gattattaag caatcaggaa tgggagtatt aagtttcgtg tttccagtaa aaccagagag
     6121 tattgaaata gctatcacct aactttatca ggcgatagca ggtagatgat gtgattacag
     6181 gttgactact gctttattta ccagccttct cggactgaag attttcggtg ttagtccaaa
     6241 gatcgtaaag attgcctttc acataaactt tgacatgcgc aacaccactg gtccacccca
     6301 tagaaaccac tttatcaact tttagcccct ttttaggcat aaagcatgat gttttaaaga
     6361 tctcttcaaa tgcttcttga tcttcgtgtt gcaaaattga acttaat
//
LOCUS       DADKXM010000176         6253 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.179, whole genome shotgun sequence.
ACCESSION   DADKXM010000176 DADKXM010000000
VERSION     DADKXM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.179"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..962
                     /locus_tag="JQR05_003645"
     CDS             <1..962
                     /locus_tag="JQR05_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY5325674.1"
                     /translation="GEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHR
                     AGYTANGVPANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEE
                     RLRKEIAELRAKIERVPFIDTFDLRYKNYEKRPDPSSQTVMFCLMDVSGSMDQSTKDM
                     AKRFYILLYLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDE
                     VVKERYNPAQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITHRAHQT
                     LWREYEHLQSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            1109..2584
                     /locus_tag="JQR05_003646"
     CDS             1109..2584
                     /locus_tag="JQR05_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY5325675.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNNVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            2863..4251
                     /locus_tag="JQR05_003647"
     CDS             2863..4251
                     /locus_tag="JQR05_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY5325676.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLQNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGFYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            4294..4797
                     /gene="yeaK"
                     /locus_tag="JQR05_003648"
     CDS             4294..4797
                     /gene="yeaK"
                     /locus_tag="JQR05_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY5325677.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(4798..4902)
                     /locus_tag="JQR05_003649"
     CDS             complement(4798..4902)
                     /locus_tag="JQR05_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325678.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            5072..5518
                     /locus_tag="JQR05_003650"
     CDS             5072..5518
                     /locus_tag="JQR05_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY5325679.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPITSGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(5475..>6253)
                     /locus_tag="JQR05_003651"
     CDS             complement(5475..>6253)
                     /locus_tag="JQR05_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325680.1"
                     /translation="HEAAVAFCIHAGTDELTSPVHQHRKGQLILALHGAITCTVENAL
                     WMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTCCTLKISPLCRELILT
                     LANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKIRTMVEMMAKGPVEWG
                     SLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQGLVKGDTVQKVAHTLG
                     YDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
BASE COUNT         1649 a         1444 c         1383 g         1777 t
ORIGIN      
        1 atggtgaagg tcaggatgaa tttgtctttc agatttcgaa agatgagtat cttgatctgc
       61 tctttgaaga tttggcctta ccgaatctga aacaaaacca acaacgccag ctgaccgaat
      121 ataaaacgca tcgggcgggt tataccgcta acggcgttcc agccaatatc agcgttgtgc
      181 gttccttgca gaactcactg gcgcgacgca cagccatgac ggcaggcaag cggcgggaac
      241 ttcatgcact ggaagagaat ttggccatca tcagcaacag tgaacctgcg cagctgctgg
      301 aagaggaacg tctgcgcaaa gaaattgcag aattacgtgc caaaattgaa cgcgtccctt
      361 ttattgacac cttcgattta cgttacaaga actacgagaa gcggcccgat ccctccagcc
      421 agacagtgat gttttgcctg atggacgttt ccggttcaat ggatcaatcc actaaagata
      481 tggctaagcg tttttatatt ctgctgtatc tgttcctcag cagaacgtat aagaacgtgg
      541 aagtcgtata catccgccat catacccagg cgaaagaagt cgatgaacat gagtttttct
      601 actcgcagga aacaggcggc accattgttt ccagcgccct gaaactgatg gatgaggtag
      661 tgaaagagcg ttataacccg gcacagtgga atatttacgc tgcacaagca tcggacggcg
      721 ataactgggc cgatgactct ccgctttgcc atgaaatcct ggcgaaaaaa ttattacctg
      781 ttgttcgtta ttacagctat atcgaaatta cccatcgtgc acatcagaca ttgtggcgag
      841 aatatgagca tctgcaatct actttcgaca actttgcgat gcagcacatc cgcgaccagg
      901 atgatattta tccggtgttc cgtgaactgt ttcataaaca aaatgcaaca gctaaagact
      961 aaaactatca gccgggtcat tatcgcctgg ctgattttta gcttactgta aattatctca
     1021 tttattacat acattagctt acaatcgctt taaatatgac agcataacct ttacacaatt
     1081 tagttccaga aaacaatcat tcggaaaaat gattcagtca acacgtattt ccctggggtt
     1141 attctttaaa tattttttat cgttaacgaa aattgatctt ggtcaaaact atatatctct
     1201 gccatcaata aaatccagca ctcacattgc tctccttttt atggtttcta tgggtacaca
     1261 aaaattaaaa gctcaaagct tttttatttt cagtttattg ctgacgttaa ttttattttg
     1321 catcaccaca ttatataacg aaaagacgaa tgtaaaactc atcccacaaa tgaattatct
     1381 gatggtcgtt gtggctttgt ttttccttaa cgccatcatt tttcttttca tgttaatgaa
     1441 atatttcact aacaaacaaa ttctaccaac actcatttta agtcttgcat ttttaagtgg
     1501 ccttatctat ttagttgaaa ctattgttgt tatccataaa cctatcagcg acagtacact
     1561 gatccagaca aagtcgaata atgtttctat tttctatatt ttccgccaac tcagttttat
     1621 ttgtttaacc tcgttggcgc tcttttgtta tggtaaagac aacatccttg acaacaataa
     1681 gaaaaaaact ggcattctgt tgctggcgct gattcctttt ttagtttttc cccttctggc
     1741 acacaatctg agcagttata acgctgacta ttctttgtat gtcgtcgatt actgtcctga
     1801 caaccatact gcgacctggg gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt
     1861 tttactgttc tttattatca tgcgtacacg attagccagc gaactatggc cattaatagc
     1921 attattatgt ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa
     1981 ttacaccatc tggtacatca gtcgcgggat tgaagtctcc agtaaactgt ttgttgtgtc
     2041 ttttctgatt tataacattt ttcaggaact gcaactctcc agcaaactgg cagttcatga
     2101 tgtgctgacc aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc
     2161 gcgacctgtt gttaaggact tctgtgtcat gctggttgat attaatcagt tcaaacgcat
     2221 caatgcccaa tggggacatc gtgtgggtga taaagtgctg gtttcaattg tcgatattat
     2281 ccagcaaagc atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt
     2341 gctatttacc gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa
     2401 tgtcgaactc ctgaccggct tcagtaacag atatgatgtt cctgaacaaa tgaccatcag
     2461 tattggcacg gttttttcaa cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc
     2521 agataaagcc ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcataatat
     2581 ttaagtgcaa aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac
     2641 tattacccct aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt
     2701 gccaggccgc aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt
     2761 tatcatgaaa ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct
     2821 gacatcttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
     2881 tatatcgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa tcagagtatt
     2941 gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtgtcgcc agaacagcgg
     3001 cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgcc
     3061 cataaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
     3121 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
     3181 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
     3241 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
     3301 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
     3361 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
     3421 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
     3481 aacaaaaaca atttgcagaa tatcttttac actcatgacc gccctctcct ctggcgtttt
     3541 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
     3601 gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
     3661 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
     3721 ttgacggcgt taattttgct gaatatggtc cggatgcact tccgtttata ccaaaatgtg
     3781 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
     3841 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
     3901 attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga gggggattta
     3961 gcgattacgt tattagctca ggcgattaaa caatcgattc gtaaaagtga ttatgccatc
     4021 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
     4081 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agagatcggc
     4141 ttctcttccg gtttttatgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
     4201 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
     4261 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
     4321 acgcatcagc agttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
     4381 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tctaggccag
     4441 ggtgctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggca
     4501 atcctcgcag ccgatcaaca ggccgatctg agccaactcg ccagccatat aggcggatta
     4561 cgcgcttcgt tggccagccc ggcggaagtt gatgaactca caggctgtgt cttcggcgcg
     4621 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct actcttcgaa
     4681 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
     4741 gcggattatt tgcgcattgc gcaaccagaa ctggtcaatt tccgccgcac tgcgtagcta
     4801 gcctgtccgt tcaataagca gaacggacaa aaccagcaca accgcaatag caaaaaacga
     4861 tgatgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
     4921 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag ttcgcctgat
     4981 tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagcg taaagtaagc
     5041 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
     5101 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
     5161 gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
     5221 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatca ccagcgggac
     5281 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     5341 agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg acgttaatgg gcagccagcc
     5401 gcaactggtt gctgggctgc tggtgggcac tgttttaggc gtagcgctgt ttcgcggcgt
     5461 accggttggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
     5521 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     5581 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
     5641 aatccctgaa gcgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     5701 gtctctttca ctatcagtcg ggcaagattg cgttcactca tcgcaaaaaa gccagcccat
     5761 tgccccaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
     5821 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
     5881 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     5941 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     6001 ggcattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
     6061 tgattgctat gcttcacccc gcccggtatc caaaccgcat attgtggcgg caccatccac
     6121 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
     6181 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
     6241 gcggcttcat ggt
//
LOCUS       DADKXM010000177         6191 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.180, whole genome shotgun sequence.
ACCESSION   DADKXM010000177 DADKXM010000000
VERSION     DADKXM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.180"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            168..495
                     /locus_tag="JQR05_003652"
                     /pseudo
     CDS             168..495
                     /locus_tag="JQR05_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(525..2636)
                     /locus_tag="JQR05_003653"
     CDS             complement(525..2636)
                     /locus_tag="JQR05_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAY5325681.1"
                     /translation="MIGAVASGNGPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIA
                     WLKQSHQYDSNLLSHQYLSLATPRYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCG
                     DANQIVHPSFFSWSSLKSLFFRQQQGNDTTVNILQANYRNGHHVTALANRLLRLKQVR
                     FSAIDRESHHFVRSCGQAEGTIRLLDDREETKQELNAKTSLSNRVAVIMMHPEQKAQA
                     RCWFSTPLVFSVQEVKGLEYETVILYNIVSAARQAFDDICEGLTPADLEGEARYSRPR
                     DRQDRSAEIYKFFTNALYVALTRATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEI
                     SSRDEWQKTAHLLEKQGKQEQADTIRSRILQTSEMPWQIITAEDARQWKQHILAGTAD
                     KTIQLQALEYSLIYSLFPLYNALYREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAV
                     LRDIERYGVDHRSPFNLTPLMSAARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLS
                     AAVSTPRYAQQKSAQLYTLLKPESLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLG
                     SALFFSEAFSAARLAECVVHLPEALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLR
                     LNHGQYILNPGLKIRQGDVWRAVYELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFY
                     ERSEKSVGYWVGGIRRAAQKA"
     gene            complement(2633..3108)
                     /locus_tag="JQR05_003654"
                     /pseudo
     CDS             complement(2633..3108)
                     /locus_tag="JQR05_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3429..3545
                     /locus_tag="JQR05_003655"
     CDS             3429..3545
                     /locus_tag="JQR05_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5325682.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3909..4751
                     /locus_tag="JQR05_003656"
     CDS             3909..4751
                     /locus_tag="JQR05_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY5325683.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAEGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4754..5842
                     /gene="capU"
                     /locus_tag="JQR05_003657"
     CDS             4754..5842
                     /gene="capU"
                     /locus_tag="JQR05_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY5325684.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5847..>6191
                     /gene="virK"
                     /locus_tag="JQR05_003658"
     CDS             5847..>6191
                     /gene="virK"
                     /locus_tag="JQR05_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY5325685.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKS"
BASE COUNT         1641 a         1444 c         1474 g         1632 t
ORIGIN      
        1 tttaccaccg ggagtttggg cataacggtg ctttcagtaa gtacgacagg tactgtggag
       61 gcacttccgt aaagaagggc atcaagctcc tgctccgaca tacctgcagg caaaggccat
      121 gaaagccctg atttccggaa gcgagcgaac agtccacaaa ctgttgaacg tggtgtcagc
      181 ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc gttctttacc
      241 cggtcaaaac agcgttgcgg tgcaccacgc ctgacggatg aactgcgtgc tcagggttac
      301 cactttaacg taaaaaccgt ggcggcaagc ctgcgccgtc aggggctgag ggcaaaggcc
      361 tcccggaagt tcagcccggt cagttaccgc gcacatggcc tgcccgtgtc agaaaatctg
      421 ctggagcagg atttttacgc cagtggtccg aaccagaagt gggcgggaga catcacgtac
      481 ttacgtacag atgaagttcg gttacatcct gtcagcacgg aacctcacgc tttttgagcg
      541 gcacgacgga tcccccccac ccagtatccg acagactttt cactgcgttc gtaaaaaccc
      601 ttcttccctc ccagcatatc cactaattcc ggtgacatat cctgcagata agtctgcaaa
      661 tcatgcccca gatcttccgg tgactgcaac tcataaaccg cgcgccacac atcaccctgc
      721 cttattttaa gccccgggtt aagaatatac tgtccgtggt tcagcctcag aaaaagcttt
      781 tttccataag gatttttgct gttaacttca tgttgagaga gctggctgct gatataactc
      841 cttcgtttgc ggcgttccgg cagcaacgct tcaggaagat gaaccacaca ctctgccagt
      901 cgtgccgcac tgaaggcttc cgaaaaaaac agagcacttc ccagatgcgt gtggaacagc
      961 gcctgcatca ggatcaccag aaacattgcc atctgtcggt tgtccagttt gatgaggcgt
     1021 ccttccacct gcagtgacag actttcaggt ttcagcaggg tatacaactg cgcacttttc
     1081 tgttgcgcat aacggggcgt actgaccgcc gctgacagaa cttgatgata agccgccagc
     1141 ccgtcattac cggtcagcaa gggatctgca ccacgctcca gcagtaactg aaccagtgca
     1201 atattacctg cccgggctgc tgacatcagc ggagtaagat taaacggcga acggtgatca
     1261 acaccataac gttcaatatc ccgcaacacg gcaaccgggt tattcattga gtatggccgg
     1321 aaatacttca gctccagtaa ctgcagagtc ttcgttctgg gctgccttgt gggcttaaaa
     1381 tcttcccggt acagtgcatt atacagcggg aacagggaat aaatcagact gtactccagc
     1441 gcctgcagtt gtatggtttt atcggctgta cctgcgagga tatgctgttt ccactgtcgt
     1501 gcatcctctg cggtgataat ctgccatggc atctcagatg tctgcaggat ccggctacgg
     1561 atggtgtccg cctgctcctg cttcccctgt ttttcaagca gatgtgcggt tttctgccac
     1621 tcatcacggc tggaaatctc ttcctgcaga ttcagaggct cttcctgatg cgtgagcgca
     1681 agcagactcc acagaggatg ctccacctgc tgttcaacaa gatacacatt gtgtgtagca
     1741 cgggtgagcg caacatacag cgcgttggta aaaaatttat agatttccgc tgaacgatcc
     1801 tgcctgtccc gcggacggga ataacgtgcc tcgccctcaa gatcagccgg tgtaagaccc
     1861 tcgcaaatat catcaaatgc ctgccgggca gcgcttacta tattgtaaag gatcaccgtt
     1921 tcgtattcca gccccttaac ctcctgaacc gaaaagacca gcggcgtact gaaccagcat
     1981 cgcgcctgtg ctttctgttc cggatgcatc ataatgaccg ctacacgatt cgaaagtgag
     2041 gttttggcat tcagttcctg ctttgtctct tccctgtcgt ccagcaaccg gatggtccct
     2101 tctgcctgac cgcagcttct gacaaaatgg tgactttccc gatcaatagc actaaagcgg
     2161 acctgtttga gtctcagcaa ccggttagcc agtgcagtga cgtggtgccc gttacggtaa
     2221 ttcgcctgca ggatattaac ggttgtgtcg tttccctgtt gctgccggaa aaagagactt
     2281 ttgagcgaac tccatgagaa aaagctcgga tgaactatct gattggcatc accacagagt
     2341 aaaaactgac cgggatgacg tagcgttttc agtaccagtt gcagttgaac aggcgtcata
     2401 tcctgaactt catcaacaaa aatgacatca taacggggag ttgccaacga aagatactga
     2461 tgactgagca gattactgtc atactgatgc gactgtttca gccatgcgat ataacgttca
     2521 aacaggacat agaccgtcgg gcggtcctcc atgccataga gcgactgccg tatccccagt
     2581 gacagatacg cctcccggga cagcggccca ttcccggagg caaccgcacc gatcacgccc
     2641 ctgaactcct catacagggt atgggcagcc cccagtgcgg cgatagccct gttgcgcgct
     2701 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2761 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2821 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     2881 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     2941 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     3001 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     3061 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     3121 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     3181 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     3241 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3301 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3361 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3421 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3481 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3541 agtagctgca ggcctgtcat gatgccggaa tagaagttcg tgtacaggtt aatgaaagcg
     3601 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagca cctgtgttgt
     3661 tctgaggtga acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt
     3721 cttagtgagt cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt
     3781 taatttagta aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg
     3841 gaaataaaat gcaccagctt tattgggttt tccttttgtc tctgcttact aggtgccaga
     3901 aactttaaat gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa
     3961 tgtatcatca tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga
     4021 agcaaatgaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
     4081 ttttttatcg gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat
     4141 accttgacaa ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata
     4201 tttttttaat taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat
     4261 attctcatcg cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc
     4321 gctggtctga agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc
     4381 atactcatac acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc
     4441 ggcaggactt gttgagcggg agagaatatt tgaaagaaat gacaggaaaa tgcagtaaac
     4501 atttgtgttg gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg
     4561 gattctatta tttatataca acagaaagaa gaatgaatgc tcctgctgaa ggaactacac
     4621 ggataggaag aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt
     4681 tttattatac aacaccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac
     4741 cggatgatta ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga
     4801 attacaggct gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt
     4861 ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa ttgggaattg atatcacatt
     4921 cgcgttattt cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca
     4981 tggttttcag cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt
     5041 agtacggtta ttcacttgga aacatccatt cagaattatc aggcaaaaga catatttgac
     5101 acgaaaaaca aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac
     5161 aagtatgaag acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc
     5221 aggctttgat ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct
     5281 ttcttggctg gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc
     5341 ggaaaaaggg cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca
     5401 gttctgttgg ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca
     5461 gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc
     5521 ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat
     5581 ggtactggca gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat
     5641 ccctgatgtt attcagaaca accagaccgg gacattgtta ccagcaggta ataagcacgc
     5701 atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg
     5761 ccaggcaaaa caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact
     5821 cacattagcg aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt
     5881 tccttaaaag gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat
     5941 tccggttcat gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt
     6001 acgaactgcg ctccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag
     6061 caagaattca tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata
     6121 ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctgaa tttttgtttc
     6181 cacaaaaatc g
//
LOCUS       DADKXM010000178         6184 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.181, whole genome shotgun sequence.
ACCESSION   DADKXM010000178 DADKXM010000000
VERSION     DADKXM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.181"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(8..358)
                     /gene="chbA"
                     /locus_tag="JQR05_003659"
     CDS             complement(8..358)
                     /gene="chbA"
                     /locus_tag="JQR05_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY5325686.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(417..1766)
                     /gene="chbC"
                     /locus_tag="JQR05_003660"
                     /pseudo
     CDS             complement(417..1766)
                     /gene="chbC"
                     /locus_tag="JQR05_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(1851..2171)
                     /gene="chbB"
                     /locus_tag="JQR05_003661"
     CDS             complement(1851..2171)
                     /gene="chbB"
                     /locus_tag="JQR05_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY5325687.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2470..2808)
                     /gene="osmE"
                     /locus_tag="JQR05_003662"
     CDS             complement(2470..2808)
                     /gene="osmE"
                     /locus_tag="JQR05_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY5325688.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3010..3837
                     /gene="nadE"
                     /locus_tag="JQR05_003663"
     CDS             3010..3837
                     /gene="nadE"
                     /locus_tag="JQR05_003663"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY5325689.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVAYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4067..4954
                     /gene="cho"
                     /locus_tag="JQR05_003664"
     CDS             4067..4954
                     /gene="cho"
                     /locus_tag="JQR05_003664"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY5325690.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(4914..5489)
                     /gene="ves"
                     /locus_tag="JQR05_003665"
     CDS             complement(4914..5489)
                     /gene="ves"
                     /locus_tag="JQR05_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY5325691.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGELSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5692..6177)
                     /gene="spy"
                     /locus_tag="JQR05_003666"
     CDS             complement(5692..6177)
                     /gene="spy"
                     /locus_tag="JQR05_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY5325692.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEECRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1582 a         1545 c         1463 g         1594 t
ORIGIN      
        1 cgactcctta tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg
       61 caagcatgga cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct
      121 taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg
      181 ccatccgtga ctgatccatc atggctttcg ctgcggcaaa atcgccctgc ttcgcctgtt
      241 tcaatgcggc atacgccagg ctacgcgctt gtccagagtt gatgataagc cccatcacca
      301 cttcttccag ctcttcagct tctgtttgcg tatcaggaat attatcgaga tccatcatac
      361 atcgttcctc tctcttctta ccggcaagat tacccgtacc ggcatcgatt aaaatttcag
      421 gggttagcga tatcttcttc gctctcttct ttatcaatcg cattttgtgc tttgttagcc
      481 accacaacaa agggcagata aattaacgtt gcgatgccaa ggttgaagag tgcgaccagc
      541 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
      601 ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc
      661 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
      721 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
      781 acctgacgat aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc
      841 gcaccactgc ccccaaggaa aataaaggag tccagcatcg gcttggctca gatatggaag
      901 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
      961 tccagcgccc acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
     1021 ataccgaaga accagagcaa tggaacaaag atcacatagg cccagcccac cacgctaccc
     1081 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
     1141 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat
     1201 gccgagaaag aacgcgatac tgaagcaggt acgctgtcgg gcagtttaat gacccaattg
     1261 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
     1321 atattcgccc cacctaacca gtttgcgcca accgcatagg cctcaccgac actatatggg
     1381 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
     1441 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat tcctaatgtt
     1501 ccgttatata cgttgccgcc aatacctttc agaccattaa gtgtttcaat ggttgaggcg
     1561 tcgaggcgaa tacctaagga ataaaaaaac gacccctccc caaagcttag aaaaacgttg
     1621 ttaattaata caaacatggc ccccgcaagg gctaacggca ttaagcgaat aaagccattt
     1681 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
     1741 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
     1801 tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
     1861 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
     1921 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
     1981 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
     2041 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
     2101 cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag ataaatgtgt
     2161 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
     2221 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
     2281 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc
     2341 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
     2401 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
     2461 ccgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
     2521 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
     2581 acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc tcacttcaga
     2641 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
     2701 caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac ccgccagcat
     2761 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
     2821 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac
     2881 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
     2941 tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
     3001 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
     3061 aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct gcaaacttat
     3121 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga
     3181 aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga atcactgcaa
     3241 tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg ccaggatgcc
     3301 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggct
     3361 agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa
     3421 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
     3481 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
     3541 ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca gttactgacg
     3601 gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct ggaagatgat
     3661 cgcccttctc tgccagatga agtggcactc ggcgtggcct atgacaatat cgacgactat
     3721 ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
     3781 accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa aaagtaataa
     3841 tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
     3901 gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg aataggcctg
     3961 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc
     4021 ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
     4081 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttaca
     4141 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
     4201 caccatgccg ctctatatcg gcaaaagcgt taatatccgt agtcgggtgc tttctcattt
     4261 acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgcac
     4321 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
     4381 gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg
     4441 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
     4501 gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg aacaaaaact
     4561 ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc
     4621 gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg
     4681 cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc
     4741 actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
     4801 gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga
     4861 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
     4921 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc
     4981 ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
     5041 agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag
     5101 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgc
     5161 gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt taccacctgg
     5221 tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg atctgcgctt
     5281 tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa
     5341 gaaagctccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
     5401 gccggaggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc
     5461 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
     5521 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca
     5581 ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
     5641 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc
     5701 agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc
     5761 attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct gggtttccat
     5821 gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt
     5881 tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcatt cttccagcgg
     5941 cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct gtttctgcgc
     6001 gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg
     6061 gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg catgggccag
     6121 gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt
     6181 ctat
//
LOCUS       DADKXM010000179         6178 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.182, whole genome shotgun sequence.
ACCESSION   DADKXM010000179 DADKXM010000000
VERSION     DADKXM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.182"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            9..161
                     /locus_tag="JQR05_003667"
                     /pseudo
     CDS             9..161
                     /locus_tag="JQR05_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1542..2489
                     /gene="icsP"
                     /locus_tag="JQR05_003668"
     CDS             1542..2489
                     /gene="icsP"
                     /locus_tag="JQR05_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY5325693.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3050..3422)
                     /locus_tag="JQR05_003669"
                     /pseudo
     CDS             complement(3050..3422)
                     /locus_tag="JQR05_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3425..3610
                     /locus_tag="JQR05_003670"
                     /pseudo
     CDS             3425..3610
                     /locus_tag="JQR05_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3653..3772
                     /locus_tag="JQR05_003671"
                     /pseudo
     CDS             3653..3772
                     /locus_tag="JQR05_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4022..4888
                     /gene="ospB"
                     /locus_tag="JQR05_003672"
     CDS             4022..4888
                     /gene="ospB"
                     /locus_tag="JQR05_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY5325694.1"
                     /translation="MNLDGVRPYCRIGNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5217..5957
                     /locus_tag="JQR05_003673"
     CDS             5217..5957
                     /locus_tag="JQR05_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY5325695.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1944 a         1160 c         1237 g         1837 t
ORIGIN      
        1 gttcctaagt ggaatgtctc cacggagaac actttatcta tcgggaaata gtgcgggcaa
       61 cagtgtttaa ttatatcgag tgcgattaca atcgctggcg acggcaccgt tggtgtggcg
      121 gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc catattacgt
      181 gggtaggctc acagagcctc acttacgact gcgtccatat tatgtgggta ggatcaataa
      241 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
      301 atgcttgata acttaattgg ggctccctct tccttttatg gtgtaattgt ttgattgaat
      361 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
      421 ttagtggaat aaggacaata gcttatccaa actgccaagc ctctttatta taagtaagat
      481 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccatt
      541 ttattgcatt taaatcccag aatagttcag tagtggtcaa tttgggaaac gcacaatatt
      601 gtacggttac gttatcgcaa tagagtgaac tcccgcccta tacggcgttt tcaaggatta
      661 gtccttaatc ggacaaccac attaaagcat aataaataat taagctttaa taatatacta
      721 tagatacagt tctattataa aaaatactat tttaggtgat gcattaaatt agtagaatat
      781 ttattctttt ataaaagcac caatgaattg tccaagtaaa catgaggtaa aatgtgtaga
      841 ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc gactttaaaa
      901 ggatgggaat tacctgtact gatagtgaga aagctttcca ttgaaataag tcaaataaaa
      961 gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg aatcggaatt
     1021 caatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat ctgaaaaact
     1081 atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca gcacacgtta
     1141 cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat tgcagccgtg
     1201 atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg atttcatatt
     1261 ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat cagcctcagg
     1321 tatgaacacc aaaaggtagg tagagaaatc tatggccccc gttactttat tcgcacaatg
     1381 aggtattttt ttatttttac aaaagttcaa taaatattat aataaaagat atgttcttgg
     1441 taacataaac aaatatttaa ttaaaataat gcttggcatt gacgtttatc ttataaagta
     1501 agaagatcat ggacatttca accaaaaaag tagagttctc gatgaaatta aaattccttg
     1561 tacttgcact ttgtgtacct gcgatcttta ctacacatgc taccactaac tatccacttt
     1621 tcataccgga caacatcagt acggatatta gtctaggatc tctgagtggc aaaacaaaag
     1681 aacgcgttta tcatcccaag gaagggggga gaaaaattag tcaactggac tggaaataca
     1741 gtaatgcgac tattgttaga ggtggcatcg attggaagct aattccaaaa gtgtctttcg
     1801 gagtttccgg ttggactact ttaggtaacc agaaagcaag catggttgat aaagactgga
     1861 acaattccaa tacccctcag gtatggaccg accagagctg gcatcccaat acgcatctcc
     1921 gtgatgctaa cgaattcgag ctgaatctta aaggttggtt attaaataat ttggattatc
     1981 gacttggact aatagcaggt taccaagaga gtcgttacag ttttaatgca atgggaggga
     2041 gttatattta tagtgaaaac ggggggaaca ggaataaaaa aggggcacat cctagtggtg
     2101 aaagaacaat aggttacaaa cagctcttca aaatacctta tattggatta actgctaatt
     2161 accgccatga gaattttgag tttggagcag aactgaaata tagtggttgg gttcgttcat
     2221 ctgatacaga caaacactat cagactgaga caatttttaa agatgaaata aaaaaccaaa
     2281 attactgctc tgttgctgcg aatattggat actatgtcac ccccagtgca aaattttata
     2341 tagaaggctc cagaaattac atttctaata aaaaaggtga tacatctctt tatgagcaaa
     2401 gtaccaatat atctggcacc attaaaaata gtgcaagtat tgaatatatt ggttttctca
     2461 cttccgcagg tataaagtat attttttgaa ttggttcacc gagttgatta cgttttcatg
     2521 gctaaaaatt gccgcccatt gtgagtcata ttcgccctga ctttccagaa ccatacgaac
     2581 tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcattctgt ttacctcttt
     2641 ctcagggcgt ttagtctcca ggatttccgg ggcggttcaa aaaactcttc gttgattact
     2701 gtggacttac tgttggcatt accgaccctg agaccggaga aataagaacc gcttagatca
     2761 tcgtggctgt tctcggggta tcaagttaca catgggcaga gcctacccaa ggtctactat
     2821 tatcgcctga aagcgctgat ggaacagtgc tatcagggac atgctgatga aaatacctaa
     2881 ataatcagcc agcactctat ctttccaaat ccacagcata gcaaagagag caaaagagcc
     2941 tgtaaattca gaaattaagg aaagcctctt ggtgcacata atctatctta tgttaaatga
     3001 aatctaattt cttgattata tgctggatcg ttcgtgggtg gctttgcccc tatatttcca
     3061 gacacctgtt atcattggcc cgctgccgca gatattcccg tggcgagcga taatccagcg
     3121 cactatgcgg atgccattcg ttataatgct cgaatgcctc tgcaaggttc tttgctgccg
     3181 ttaacccgtc aggtttgggc atgatactga tgtagtcaca ctttattgtt ttcacgaagc
     3241 tctttgctat tctgttactc tccggactcc gcaccgccgt gctcttcggt tcaacaaagt
     3301 cccaacatcc gggtgaactg gcgtgtttca ttagcccggt agcatgaacc attatccgtc
     3361 agtcacttca ctggagacgc cggaagctcg ttgccgaagc ggtgttccac cgctcccagc
     3421 atgtcgccaa cgggagagca tctacggtct gattgaactg tttattcaaa ttgtccaccg
     3481 tctgtcagta cgctctgaac gtcggctggt taaaacactg ctcgctgatt ttcagagggt
     3541 tcacggtaaa acagcacttt tattccggat tgcggaagcg gctctgaata atccggatgg
     3601 gctggttaaa atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
     3661 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
     3721 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
     3781 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
     3841 tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
     3901 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
     3961 tatgaaaata taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg
     4021 tatgaattta gatggtgtta gaccatactg tagaataggc aataaaaaga atgaaagcat
     4081 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
     4141 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     4201 tataatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     4261 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     4321 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     4381 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     4441 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     4501 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     4561 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     4621 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     4681 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     4741 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     4801 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     4861 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     4921 catttaagat gaagttattt tacctttagt agaaaagtgc ttccctatga gggaaaatca
     4981 caaatcatca taatacagag acaaaacgat acgaacaaat agataaaaaa catcattctt
     5041 ttaccacatt atttttcgtg aatatgaaaa ataatgttat tactttaata taagactatt
     5101 ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtatttt ttgtaacaca
     5161 gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga
     5221 aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa
     5281 atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac
     5341 ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct cattatgaat
     5401 tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg
     5461 agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc
     5521 caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta
     5581 acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta
     5641 cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct
     5701 ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac
     5761 ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg
     5821 atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg
     5881 aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca
     5941 atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa
     6001 tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc cttgaattta
     6061 agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg
     6121 ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctagga
//
LOCUS       DADKXM010000180         6088 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.183, whole genome shotgun sequence.
ACCESSION   DADKXM010000180 DADKXM010000000
VERSION     DADKXM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.183"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..600)
                     /gene="ybjI"
                     /locus_tag="JQR05_003674"
     CDS             complement(<1..600)
                     /gene="ybjI"
                     /locus_tag="JQR05_003674"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY5325696.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQ"
     gene            complement(600..1808)
                     /locus_tag="JQR05_003675"
                     /pseudo
     CDS             complement(600..1808)
                     /locus_tag="JQR05_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1892..2428
                     /locus_tag="JQR05_003676"
     CDS             1892..2428
                     /locus_tag="JQR05_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5325697.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(2603..4288)
                     /locus_tag="JQR05_003677"
     CDS             complement(2603..4288)
                     /locus_tag="JQR05_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY5325698.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            4558..4935
                     /locus_tag="JQR05_003678"
     CDS             4558..4935
                     /locus_tag="JQR05_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325699.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(4965..5222)
                     /gene="grxA"
                     /locus_tag="JQR05_003679"
     CDS             complement(4965..5222)
                     /gene="grxA"
                     /locus_tag="JQR05_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY5325700.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            5382..5669
                     /locus_tag="JQR05_003680"
     CDS             5382..5669
                     /locus_tag="JQR05_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY5325701.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            5653..>6088
                     /gene="nfsA"
                     /locus_tag="JQR05_003681"
     CDS             5653..>6088
                     /gene="nfsA"
                     /locus_tag="JQR05_003681"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY5325702.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKL"
BASE COUNT         1638 a         1537 c         1474 g         1439 t
ORIGIN      
        1 ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat cgatgctgcc
       61 gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg ctttttgtac
      121 ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat cttctaagtt
      181 gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tccgccaccg ttttcatggc
      241 atcgtcatat tttttgagtg tataggcgct attttttccg caggcaataa tttccacttc
      301 cgggcgcgtc agcaaatgtt ccacgacagt agcaaacgca tccttcgaca gctcgccatt
      361 aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa aggcaatttc
      421 attcgcaatt tcagggaaga aagagatcaa ctgataatat tgattcccgc tggcgaccac
      481 aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct cacggttata
      541 ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt taatgctcat
      601 caactattct ccatcgccgt ctgcgttttg gtatcgggtt tggcgacggc tttcgcgaca
      661 atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa tccataatgt
      721 tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc ggtcgttgct
      781 accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga aatggtcagc
      841 gggaagccca gcgaggcacc cagtccccaa agtacaacag acactcctgc gacccaggcg
      901 ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact ggcccgaacc
      961 acggcaacgc gactgtaacg gtcgatgaac caaccgccgg tgaagcgtcc aacggtcatc
     1021 cccagggtaa aaccggcata aatcagtgag ccggaagtgg ggctaaaacc gtgaccatca
     1081 accattaata agggtaacta gtcgttggca gaaccttcgg caaaggccat cgccagcact
     1141 acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt ttcgccatgc
     1201 tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat ggcgatataa
     1261 ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg aacgccaaag
     1321 gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ccaggctata aaaaccgtgc
     1381 atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc aacgtttatc
     1441 gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc aaagagcagg
     1501 ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc gcaggacatc
     1561 gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga gcagagaata
     1621 ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc gatcgagaca
     1681 gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa caggcctggc
     1741 aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg tgaagaattt
     1801 acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag gttgtgtaca
     1861 tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc cgcaacggcg
     1921 agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac atgctgttac
     1981 gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct actatttttc
     2041 aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta tgtcccggca
     2101 atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg ctatcaccga
     2161 tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt accagctcta
     2221 cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga tgcagcgcag
     2281 tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg atgcgtttat
     2341 tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg aggagatttt
     2401 gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa aaacccatca
     2461 accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc aaagaaaagc
     2521 agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc aaaaaaattt
     2581 cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg atgattgtcc
     2641 ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc agcgcaggga
     2701 tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg cgtgcgccca
     2761 tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg aacaagaaac
     2821 agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga ccgccaatcg
     2881 cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc ataaacacca
     2941 tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg aaggtcgggt
     3001 ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca gcgttcccca
     3061 tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc ccaataacaa
     3121 agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata aagccgatgc
     3181 gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg tcacctttgt
     3241 taagtacgac gttgtcatct atcggcatct caatctggct acgaatgacg cggttaagga
     3301 agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg ttatggtttt
     3361 taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg aaaacttctt
     3421 taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct accaacgcta
     3481 tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc ccgttacgtc
     3541 gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc agatttttgc
     3601 cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc ggtaaataaa
     3661 ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg gcgctggtct
     3721 gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa ctcaccagac
     3781 cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt gagagctgcc
     3841 tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc ggtgtcgacg
     3901 tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac agcttaccta
     3961 accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt aggtaatttt
     4021 tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg acgcagaaaa
     4081 taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt tgttggccta
     4141 ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa ccaagtcgta
     4201 actttccgag acatagccca agcgcgagga ccacaaataa taacagaatg taattcccat
     4261 ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact agtaagctgt
     4321 tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc agattcattc
     4381 agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca cattaggtat
     4441 caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg gctttatctt
     4501 actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga ggaacgagtg
     4561 aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat tgtggtgtgc
     4621 ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc tgaaatcggc
     4681 ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg tagagaagtc
     4741 ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat tatgcggctg
     4801 tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag cgcggtgttc
     4861 tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa accacagcac
     4921 agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc gtccagattt
     4981 tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc gacaaaaatc
     5041 tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt agtgatccct
     5101 tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct caatttctca
     5161 gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat aacggtttgc
     5221 attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt attcgctgaa
     5281 agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca caatacagtg
     5341 aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg atcggtaaat
     5401 tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg gtggcgctgc
     5461 tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa gtgcagattc
     5521 tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt attcttcagg
     5581 tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca cgttcagaaa
     5641 gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc cattcgccat
     5701 ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag cgcccgtgcg
     5761 acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga caaagcatta
     5821 cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc ggcggagttc
     5881 tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc tcagctcggc
     5941 ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca gaatgcatta
     6001 accgcagcgg aatcgctggg attgggcggg gtatatatcg gcggcctgcg caataatatt
     6061 gaagcggtga cgaaactgct gaaattac
//
LOCUS       DADKXM010000181         6069 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.184, whole genome shotgun sequence.
ACCESSION   DADKXM010000181 DADKXM010000000
VERSION     DADKXM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.184"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..566
                     /gene="yneK"
                     /locus_tag="JQR05_003682"
     CDS             <1..566
                     /gene="yneK"
                     /locus_tag="JQR05_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY5325703.1"
                     /translation="HTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDP
                     NLITAILDITDSELKEDLLQSLMDSLGSNKHVQPKVCICFLSLLAEQESPHFQDLFLF
                     FANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHED
                     LVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            716..1906
                     /gene="ydeA"
                     /locus_tag="JQR05_003683"
     CDS             716..1906
                     /gene="ydeA"
                     /locus_tag="JQR05_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY5325704.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(1931..2596)
                     /gene="marC"
                     /locus_tag="JQR05_003684"
     CDS             complement(1931..2596)
                     /gene="marC"
                     /locus_tag="JQR05_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY5325705.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2808..3242
                     /gene="marR"
                     /locus_tag="JQR05_003685"
     CDS             2808..3242
                     /gene="marR"
                     /locus_tag="JQR05_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY5325706.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3262..3645
                     /gene="marA"
                     /locus_tag="JQR05_003686"
     CDS             3262..3645
                     /gene="marA"
                     /locus_tag="JQR05_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY5325707.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3677..3895
                     /gene="marB"
                     /locus_tag="JQR05_003687"
     CDS             3677..3895
                     /gene="marB"
                     /locus_tag="JQR05_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY5325708.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3926..4753)
                     /locus_tag="JQR05_003688"
     CDS             complement(3926..4753)
                     /locus_tag="JQR05_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY5325709.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVGS"
     gene            4948..>6069
                     /gene="ydeE"
                     /locus_tag="JQR05_003689"
     CDS             4948..>6069
                     /gene="ydeE"
                     /locus_tag="JQR05_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY5325710.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVII"
BASE COUNT         1546 a         1528 c         1394 g         1601 t
ORIGIN      
        1 accataccgc tgcagattac ttacgccagt ttgatttgct tgtcctgaat tttatctctc
       61 gcggtaattt tgtcatactc ccccgattat ggaataactc tgaggttcac agatggttcg
      121 tcaataaaga tcctaacttg atcaccgcca ttctcgacat aacggacagc gaattaaaag
      181 aggatttatt gcaaagcctg atggattcat taggttctaa caaacatgta caacccaaag
      241 tctgcatctg cttcttatcc cttttagcag aacaagaatc tcctcatttt caggacttgt
      301 ttttattttt cgccaatatg ttattgcact atcaccaatt tatgaatccc aatgaaagtg
      361 atctgaatga cgtgttgatg ccagcatcat taagtgatga taaaattatc aaacatatgg
      421 cgcgcaggac cctcaaactg tttgtaaaaa atgaaacgcc gccaaaagtt actcacgaag
      481 acctggtgaa aaacaggcct cgctcccctg tcagaccacc tatacccgca accgccaaaa
      541 cgccagacct ccctgaacgt cattaaaccg tgatgttacc gactctctga cgcggaacag
      601 aatcagcgtc agagaaacgg aaaacgcgat ccagatcaca aatgcattgt attcacatca
      661 ttaaccgttt taagatcatt tcatcacttt ttcgcaactc acccgataat ctgttatgac
      721 aacaaacact gtttcccgca aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc
      781 cttcatcttc aacaccaccg aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag
      841 ttttcacatg caaaccgctc aggtcggcat catgttgacc atttacgcat gggtagtagc
      901 gctaatgtca ttgcctttta tgttaatgac cagtcaggtt gaacggcgca aattactgat
      961 ctgcctgttt gtggtgttta ttgccagcca cgtactgtcg tttttatcgt ggagctttac
     1021 cgttctggtg atcagtcgca ttggtgtggc ttttgcacat gcgattttct ggtcgattac
     1081 ggcgtctctg gcgatccgta tggctccggc cgggaagcgg gcacaggcct tgagtttaat
     1141 tgccaccggt acagcactgg cgatggtctt aggtttaccg ctcgggcgca ttgtggggca
     1201 gtatttcggc tggcgaatga ccttcttcgc gattggtatt ggggcgcttg ttaccctttt
     1261 gtgcctgatt aagttacttc ccttactgcc tagtgagcat tccggttcat tgaaaagcct
     1321 cccgctattg ttccgccgcc cggcattgat gagcatttat ttgttaactg tggtggttgt
     1381 caccgcccat tacacggcat acagctatat cgagcctttt gtgcaaaaca ttgcgggatt
     1441 cagcgccaac tttgccacgg cattactgtt attactcggt ggtgcgggca ttattggcag
     1501 cgtgattttc ggtaaactgg gtaatcaata tgcgtctgcg ttggtaagta cggcgattgc
     1561 gctgttgctg gtgtgtctgg cactgctgct acctgcggcg aacagtgaaa tacacctcgg
     1621 ggtgctgagt attttctggg ggatcgcgat gatgatcatc gggcttggta tgcaggttaa
     1681 agtgctggcg ctggcaccag atgctaccga cgtcgcgatg gcgctattct ccggcatatt
     1741 taatattgga atcggggcgg gtgcgttggt aggtaatcag gtgagtttgc actggtcaat
     1801 gtcgatgatt ggttatgtgg gcgcggtgcc tgcttttgcc gcgttaatat ggtcaatcat
     1861 tatatttcgc cgctggccag tgacactcga agaacagacg caatagttga aaggcccatt
     1921 cgggcctttt ttaatggtac gttttaatga tttccaagat gccgttaata ataaactgca
     1981 cacccataca taccagcagg aaccccatca ggcgggagat cgcttcaatg ccacccttgc
     2041 ccaccagccg cataattgcg ccggagctgc gtaggcttcc ccacaaaata accgccacca
     2101 ggaaaaagat cagcggcggc gcaaccatca gtacccaatc agcgaaggtt gaactctgac
     2161 gcactgtgga cgccgagcta ataatcatcg ctatggttcc cggaccggcg gtacttggca
     2221 ttgccagtgg tacaaaggca atattggcac tgggttcatc ttccagctct tccgacttgc
     2281 ttttcgcctc cggtgaatca atcgctttct gttgcggaaa aagcatccga aaaccgataa
     2341 acgcgacgat taagccgcct gcaattcgca gaccgggaat cgaaatgcca aatgtatcca
     2401 tcaccagttg cccagcgtaa tacgccacca tcatgatggc aaatacgtac accgaggcca
     2461 tcaacgactg acgattacgt tcagcactgt tcatgttgcc tgccaggcca agaaatagcg
     2521 cgacagttgt taatgggtta gctaacggca gcaacaccac cagccccagg ccaattgctt
     2581 taaacaaatc taacattggt ggttgttatc ctgtgtatct gggttatcag cgaaaagtat
     2641 aaggggtaaa caaggataaa gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa
     2701 cttgaaccga tttagcaaaa cgtggcatcg gtcaattcat tcatttgact tatacttgcc
     2761 tgggcaatat tatcccctgc aactaattac ttgccagggc aactaatgtg aaaagtacca
     2821 gcgatctgtt caatgaaatt attccattgg gtcgcttaat ccatatggtt aatcagaaga
     2881 aagatcgtct gcttaacgag tatctgtctc cgctggatat taccgcggca cagtttaagg
     2941 tgctctgctc tatccgctgc gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt
     3001 cggtcgacct gggagcactg acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg
     3061 aaaggttgcc gaacccgaat gataagcgcg gcgtactggt aaaacttacc accagcggcg
     3121 cggcaatatg cgaacaatgc catcaattag ttggccagga cctgcatcaa gaattaacaa
     3181 aaaacctgac ggcggacgaa gttgcaacac ttgagcattt gcttaagaaa gtcctgccgt
     3241 aaacaaaaaa gaggtatgac gatgtccaga cgcaatactg acgctattac cattcatagc
     3301 attttggact ggatcgagga caacctggaa tcgccactgt cactggagaa agtgtcagag
     3361 cgttcgggtt actccaaatg gcacctgcaa cggatgttta aaaaagaaac cggtcattca
     3421 ttaggccaat acatccgcag ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt
     3481 aacgagccga tactctatct ggcagaacga tatggcttcg agtcgcaaca aactctgacc
     3541 cgaaccttca aaaattactt tgatgttccg ccgcataaat accggatgac caatatgcag
     3601 ggcgaatcgc gctttttaca tccattaaat cattacaaca gctagttgat aacgtgacaa
     3661 cgtcactgag gcaatcatga aaccactttc atccgcaata gcagctgcgc ttattctctt
     3721 ttccgcgcag ggcgttgcgg aacaaaccac gcagccagtt gttacttctt gtgccaatgt
     3781 cgtggttgtt cccccatcgc aggaacaacc accgtttgat ttaaatcaca tgggtactgg
     3841 cagtgataag tcggatgcgc tcggcgtgcc ctattataat caacacgcta tgtagtttgt
     3901 tctggccccg acatctcggg gcttattaac ttcccacctt taccgcttta cgccaccgca
     3961 agccaaatac attgatatac agcccggtca taatgagcac cgcacctaaa aattgcagac
     4021 ccgttaagcg ttcatccaac aatagtgccg cacttgccag tccgactacg ggcaccagtg
     4081 tcgccacaaa cgccagatac atcagcgaca agatggtggt catatcaata gtaaccagac
     4141 tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag ggaatgattg
     4201 ggattaaagc gctccagatt accagcgaca tcaccgccgg acgcgttgag tgcgacatga
     4261 tctttttatt gaagatgttg ccacacgccc aactaaatgc tgcagccagg gtcaacataa
     4321 agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc agtacgccaa
     4381 aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg aaagtaaacg
     4441 cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc agtccagcag
     4501 gcataccaaa gttaatggcg cagaacagaa aggcaaactg cgcaaaactg atggttaatc
     4561 cataccccaa gagcaaattc agtggtactt tcggtcgtgc aacaaaaaag atggccggaa
     4621 aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta tgaagcccca
     4681 ctttgatgac cacaaaattt agcccccata cgaccactac cagtagcgcc aacaccccat
     4741 cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca acttagctga
     4801 atttactttt ctttaacagt tgattcgtta gtcgccggtt acgacggcat taatgcgcaa
     4861 ataagtcgct atacttcgga tttttgccat gctatttctt tacatctcta aaataaaaca
     4921 taacgaaacg cactgccgga cagacaaatg aacttatccc tacgacgctc taccagcgcc
     4981 cttcttgcct cgtcgttgtt attaaccatc ggacgcggcg ctacgctgcc atttatgacc
     5041 atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc gatgacaatt
     5101 gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga taagttcgac
     5161 aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat tgccattcct
     5221 ttagtgaata acgtgacgct ggttgtgctc ttttttgccc tcattaactg cgcctattct
     5281 gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac cagcaaaacg
     5341 aaaatcttct caatcaacta caccatgcta aacattggct ggaccatcgg tccgccgctc
     5401 ggcacgctgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc tatctgttcc
     5461 gcgtttccca tgcttttcat tcaaatatgg gtaaagcgca gcgagaaaat catcgccacg
     5521 gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact gttgtggttt
     5581 acctgctctg gttttctggc ttcttttgta agcggcgcat ttgcttcatg catttcacaa
     5641 tatgtgatgg tgattgctga tggggatttt gccgaaaagg tggtcgcggt tgttcttccg
     5701 gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgact taacccagct
     5761 aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcggact ggtcggtttt
     5821 attttttccg gcaacaacct gctattgtgg ggtatgtcag ctgcggtatt tactgtcggt
     5881 gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc gccaggaatg
     5941 aaagccagct atttttccgc ccagtcttta ggctggcttg gtgccgcgat taacccatta
     6001 gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat cttagcgttg
     6061 gtgatcatt
//
LOCUS       DADKXM010000182         5986 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.185, whole genome shotgun sequence.
ACCESSION   DADKXM010000182 DADKXM010000000
VERSION     DADKXM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.185"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..442)
                     /locus_tag="JQR05_003690"
     CDS             complement(<1..442)
                     /locus_tag="JQR05_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5325711.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     VSEAIQANIELLSENRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLNDISTKEVAAMLNTYVAEGKAASAKLIRSTL"
     gene            complement(420..638)
                     /locus_tag="JQR05_003691"
     CDS             complement(420..638)
                     /locus_tag="JQR05_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY5325712.1"
                     /translation="MYLTLKEWNARQLRPRNPETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(743..931)
                     /locus_tag="JQR05_003692"
     CDS             complement(743..931)
                     /locus_tag="JQR05_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY5325713.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(982..1326)
                     /locus_tag="JQR05_003693"
     CDS             complement(982..1326)
                     /locus_tag="JQR05_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325714.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            complement(1466..2887)
                     /locus_tag="JQR05_003694"
                     /pseudo
     CDS             complement(1466..2887)
                     /locus_tag="JQR05_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            complement(2911..3207)
                     /locus_tag="JQR05_003695"
     CDS             complement(2911..3207)
                     /locus_tag="JQR05_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAY5325715.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHSFIQISQKENVTVIIDD
                     GYVLGTFDCCVCAIKEIALLTARIEEADKKYGISYQRLKELSHIASSVRFFYAI"
     gene            complement(3282..3569)
                     /locus_tag="JQR05_003696"
     CDS             complement(3282..3569)
                     /locus_tag="JQR05_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325716.1"
                     /translation="MDMNPMLDYCFTPALRQTISHTGIKKWLWRIRKILQQKGDPDLI
                     QCDYHLCNKQFLPEKAFTGELIYMHGNMIARKKRKYCGAACAEKDKMAHEL"
     gene            complement(3580..4419)
                     /locus_tag="JQR05_003697"
     CDS             complement(3580..4419)
                     /locus_tag="JQR05_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5325717.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESLECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
     gene            complement(4841..4993)
                     /locus_tag="JQR05_003698"
     CDS             complement(4841..4993)
                     /locus_tag="JQR05_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY5325718.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(5187..5594)
                     /locus_tag="JQR05_003699"
     CDS             complement(5187..5594)
                     /locus_tag="JQR05_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5325719.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEVRKRNKHQ"
     gene            5672..5899
                     /locus_tag="JQR05_003700"
     CDS             5672..5899
                     /locus_tag="JQR05_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5325720.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQK
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
BASE COUNT         1456 a         1364 c         1372 g         1794 t
ORIGIN      
        1 caagggttga cctgattaat tttgcggaag ccgctttacc ttctgctacg taggtgttta
       61 gcattgctgc cacctctttc gttgatatgt cattgagcgg tttgtccggc aattttcttc
      121 gaatcgccct gattttgctg gcgtagtcga gtagagtttt cggtctgata cccctctcgc
      181 tgaggattgt ttcatatcgg tcaagccacg catgaagagt gattgcgtca gcgcctttaa
      241 tcctgtctat cagtgactca cgcctgttct cagatagcaa ctcaatattg gcctgaatag
      301 cctctgaaac tgctatcctt ctgtctctgc ctaatccgaa ctctttaccc gtccttgggt
      361 ccctgtagca gtaatatcca ttgtttctta tataaaggtt agggggtaaa tcccggcgct
      421 catgacttcg ccttcttccc atttctgatc ctcttcaaaa ggctacctgt tactggtcga
      481 tttaagtcaa cctttaccgc tgattcgtgg aacagatact ctcttccatc cttaaccgga
      541 ggagggaata tcctgcactc gcgtacccag cggcgaaccg tttccgggtt ccgtggacgt
      601 aactgacggg cgttccattc tttgagcgtc aaatacatgt catatctctg caatatgacc
      661 gccagtaaat atacagaata ctggcgggtg tggttgattt ttaataatca gctatgaagt
      721 tctaatctgg atataatgaa attcacgagg actgaagttt ctcgcaatta aaatttatca
      781 gttttacttt ctgctctctg gaaacgcctg cttctttttc ccctgagagc attttttcgc
      841 attctgattt cgttagtttt gtctttgagt atcttgtcca gctggtagga gatccacctt
      901 ccttttcaat agtggctgta attttataca tgaacacctc cattaatatt ttcaaaaacc
      961 gtgtccaaaa tgcacttggt tttacaccgg aatattctca tcgtatcctg cttgttgcgt
     1021 ttcgttttgt gtttgtggat ccgggaatgc atattcaacg ccttccagtt caatccatag
     1081 tgcattacga ccagctttga ttgtcggcca gtccatacct ttgattcttt cccatgagcg
     1141 ggaggtgaat gttttttcca gcaaatcagc ttttgcgcgt ttggcgtcgt tactggtgcc
     1201 cccatggtgc ttgttgagca actcgataat ttcatcaagt gcgatttctt ttgcacgctt
     1261 ttcttttagc catgtcggtg taccgtcgtt ggcaaataat tcactgttat cccgtgaggt
     1321 atcgatgccg aggtgttcac ctcccaggtt aagaaactcg atatgaggaa gaaagttttt
     1381 aaattttgac ttcatttgag gagatatgat gaccgggaat ccatttcgaa tcattcgggt
     1441 cgctaatccc ttcaacaaat tctccgcgag aggcagccag taatttgtct gcatcgacag
     1501 gatttttggg cggaatgttt ttccgggctt catggagttc tgtccgcagt ttctgatatt
     1561 tcgcatcaac agcatttacc tgtgactgag catccagcgg ctgcgtgtcc tgatgatgtt
     1621 cagttgcatc cggttccact gtttcagccg ttgcctgttc atctgccatt gcgcaagatg
     1681 gttgcggttt ttcttcatca tcctgttttc cttcttctgt tacacgttgc ggcatcgggg
     1741 cagaggagcg accgcaggca atatccacga tttccggatc agggttggca tgatcggttt
     1801 cagtcagtac cttgttcaga tattcagtga cgtgcgcggg gatgacctcg ataccaatcg
     1861 gtgcatcttt cactgacgca accacgatgg cgcgggaata atccatcccg ccaggcatgg
     1921 tgatgaattt gtcgcggaaa acagaaaagg gcggtttatt ttcagcgata atttcctcga
     1981 cacgtttagc gtgtgccgga tgaaggttat aaatgtccac gtccattgaa cgagccagta
     2041 cgccagtggc tacgtcgcgc gccagtgacg tcagatcgtg aacgaaacct tcgccgcgat
     2101 cggtgaggtt cccgccgcca gcattagcac cggaagccgt gcgagtgata cgcgaaacac
     2161 gatttccttt catccactct tttgtcagca gaccgcgatc ggtgtagttg gcatccagat
     2221 atgcttcgaa aaaagcagtc atcagtccca gatttgaatt gccaggatta gggaaaactt
     2281 tgtcagtatc acgcacgagt ttgtggaggt cgcgaatctc cagcgggtcg agcagactgg
     2341 ttttatgcga aacagccaga gcagtaacag ccggtagttc ttcatcccga gcaatgtgta
     2401 atgcctggag ttcgtcgcgt gaaacgtgcg ttaccggttt ttcgctgccg tgttgcgcaa
     2461 gccaacgaat gggcagttcc tgaccggaaa ctggcaggag catgttctcc tcaatttctg
     2521 tcatgtcttc gccgttgacg ttggtattgt cagtgctggc tggtttgtcc tgaacagagg
     2581 gagaagatgc gataaatacc attgtgatgc catcttcccc gcctttttcg taacggttgc
     2641 agaattccgt atcaaacacg ccttctggtg ggaggtcatc aacaacgggc aaattgacgc
     2701 gaacaggttt tttaaagtca tcttcatcgt agccagcatc gtcaatcgca acagcaccac
     2761 gggagatggc aatggataat tttttcgctt cagcccagta aaaaccgcct ttaataccga
     2821 ggcgttttct tactttgtca ttttttgctt cgtaatacag tgggtaaact tgtttatcgg
     2881 tgctcattgt tttttaacct caactcatat tcagattgca taaaagaaac gaactgatga
     2941 tgcgatatga gagagttctt tcagtcgttg ataactgata ccgtatttct tgtcagcctc
     3001 ttcaatgcga gctgtgagta atgcaatttc tttaatggca catacacaac aatcaaatgt
     3061 tcccagaacg taaccgtcat caataattac cgtgacgttt tctttttggg aaatctgaat
     3121 gaaactatgt aatgtattcc cacatttaaa ttcggcgaga gaatcgttaa taacatcaag
     3181 ttcaatttca cattcaataa tgctcattgc tgtttccttt tttaaggttg agtgaatccc
     3241 tgccattgct ggcataaatt cagtttcgaa tattcagtta attaaagttc gtgtgccatc
     3301 ttgtcttttt cggcacaagc tgcaccgcaa tattttcgtt tctttctggc aatcatgttt
     3361 ccatgcatat aaataagctc gccagtaaaa gctttttcag gtaaaaactg tttattgcag
     3421 agatggtaat cacattgtat taaatccggg tcaccttttt gctggagaat ttttctgatt
     3481 ctccacagcc attttttaat cccggtatga ctgatggtct gcctgagggc aggtgtaaaa
     3541 caataatcaa gcatggggtt catatccata gctccatttt tattttaagg aaagcaactg
     3601 ctctacggtc atgtttttaa ttgcgccccg gtttacaaga gtccagccct gtttttccag
     3661 ataaaaccgg aaagtctcca gggtacagac cagtgcgcca tcaggaacgg tttcggtgaa
     3721 ttcgacattg ccgaatttgt cgaagtgaac aaccagagtg cgaccatcac ccggaatcat
     3781 cttgtcagca ggtggggtgt tattctggcg cagttcggcc tccatgcggt cgaactcagc
     3841 aatgtaggct tccttgaatg tggcggcttt tttgcccgtg aagcccatca ccaggaaaac
     3901 gaagccgttt ttggtgattt ggtaggcgtt ataagaattg ccgcggtgtt cgaatttaac
     3961 ccgcgaaaag ttgctggtta aaaatagttc agaacattcg agggattcta ttttttgaac
     4021 aacatggtga tgctgcttgc caaaaaactc agcaatcgaa atagaagtag tgacggcgcg
     4081 accattttcg atggttacgt cagggtgaga aagggtagta gccataatga catcctcaag
     4141 tgataagtta attaactcac cagcagaggt gctaatctca tgggtggtga gacgcacagg
     4201 gttagcacta ccggtcactc gagaacccgg ccagccttgc ggctgccccg cacgcctcac
     4261 cataatttga atgtggctgt gcattacgca taaaaaaacc gcttcagcgc ggttatgcgc
     4321 tcgagtaact ttcggggtgc taatcccggc acccgtttta tgaggtgcag gtgcactata
     4381 attccacccg ttctggtttt caatagctac attcaacatt ttctctttcc tttcatcacc
     4441 gaagtgaatt ttgtgatgcg gtgcctggtg cctccaggtg acgttaacca gttaacaatt
     4501 acagtcggct ttcccaccca aaacaataag gactaacatg acttttaact gtgccgcgtg
     4561 cgcttagccg cattcaccgc atcacaaaat tcactttaaa aagggcggac atcagccagc
     4621 aattaaaccg atgccgccaa ctggtacttc acacagcaat gtcgttattt acaaccggaa
     4681 gcgcactccc accatttaaa tttcacagac aagaccgact ctttatgaat accggaaatg
     4741 cgccttcgtg ttgtgcccgg ttttatttca ccacctccgg gctttggtgg cctcggctat
     4801 acctctacag cgagaatatt gaattaatcc aataaatgga ttagctggta tttttggcaa
     4861 gccagcgacg tgcgccagct tcggttttaa acgttttgct tttggtatac gtcatggcgg
     4921 tgaatgtgcc gtcctgattg ggaaacacgc cacacactag ggattcgttg ttgccaagat
     4981 cgatagtatc catgctgacc tcatttcccc ttaacgccgg ggtggcggaa ctgtttactg
     5041 agagcaccgt gcggtgtctt gatagatgtt aatttagttt tctcatggta attggtcaag
     5101 tatttttgat gagaaatctc aatatttaat gcaaaataaa gccaatacat tgaaatgtaa
     5161 ggctttaaaa tttgtgaagg ggggggttat tgatgtttgt tacgtttgcg aacttctagt
     5221 agctcggtga ataggcgatt aaaattctca acgcgggcac ggagttcgct gatttgtgct
     5281 tgctgctctg attttggaag tgcgcgatac aatcgcaaca tctccaactc atcttccgat
     5341 aagtctaagg cgctgttgag tgcaactggt ggatctggtg ttttatcctc gtcaccaaac
     5401 agtatccaag ttggtgaaca ttgcaatacc tcagccaggc gatgcaaatt ttgcccgcgc
     5461 ggggctgtat ggtcgctttc ccatagtgaa attgatgagc cagatacgcc agcagctttg
     5521 cttaaatcgt tttgacttaa accaacctgt ttgcgtcttt ctctaattcg ttgacctaaa
     5581 gttttctcgt tcatatttag atatcttaat aacccttgac ttgagattcc ttgagtgatt
     5641 actattgaga aaactcaact ctggaggggt gatgtttaaa tcagacgtaa ttaattttta
     5701 tgggacgaaa gccaaagtag cgaaagctgc tggtgttgat ccatctgctg tttctcaatg
     5761 gggtgaactg gttcccgaag gtcgcgcgat gcgcctacaa aaagcatctg gcggggagct
     5821 tcagtacgac cccaaagttt atgacgaata tcgtaaggca aaacgacctg ggaaggtgat
     5881 tcatgaaaat caggcatgag cacatcgaat cagtgctgtt agccctagca gccgaaaaag
     5941 ggcaggcgtg ggtcgctaac gcaattactg aagaatatct gcgcca
//
LOCUS       DADKXM010000183         5900 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.186, whole genome shotgun sequence.
ACCESSION   DADKXM010000183 DADKXM010000000
VERSION     DADKXM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.186"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(78..830)
                     /gene="yecC"
                     /locus_tag="JQR05_003701"
     CDS             complement(78..830)
                     /gene="yecC"
                     /locus_tag="JQR05_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY5325721.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(827..1495)
                     /gene="tcyL"
                     /locus_tag="JQR05_003702"
     CDS             complement(827..1495)
                     /gene="tcyL"
                     /locus_tag="JQR05_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY5325722.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1510..2496)
                     /gene="dcyD"
                     /locus_tag="JQR05_003703"
     CDS             complement(1510..2496)
                     /gene="dcyD"
                     /locus_tag="JQR05_003703"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY5325723.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2601..3401)
                     /gene="tcyJ"
                     /locus_tag="JQR05_003704"
     CDS             complement(2601..3401)
                     /gene="tcyJ"
                     /locus_tag="JQR05_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5325724.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3489..4040)
                     /gene="fliZ"
                     /locus_tag="JQR05_003705"
     CDS             complement(3489..4040)
                     /gene="fliZ"
                     /locus_tag="JQR05_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY5325725.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4086..4805)
                     /locus_tag="JQR05_003706"
     CDS             complement(4086..4805)
                     /locus_tag="JQR05_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY5325726.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNEREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4970..>5900)
                     /locus_tag="JQR05_003707"
     CDS             complement(4970..>5900)
                     /locus_tag="JQR05_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY5325727.1"
                     /translation="TNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDTAKGTITIDG
                     SAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAANNTKATTIDI
                     GGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGYSVNGTTGAV
                     TKGVDSVYVDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTTDAETKAATT
                     ADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEV
                     SNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
BASE COUNT         1392 a         1569 c         1439 g         1500 t
ORIGIN      
        1 atttgttaag cagttttatc acgcttgaaa tggcgtcccg ccccgacagg tgaatcgtcg
       61 gggctgattt tttcttatta ttgcagcaga agcttctcga ggaactggcg ggtgcgaggc
      121 tgctggggat cggcaaataa cgcttttgct gccccctgct cgactatccg cccctggtcc
      181 ataaagatcg cccggtccgc aacatcccgg gcaaagctca tttcgtgtgt cacaatcacc
      241 atcgtgcgct tttcctgcgc cagctggcgg atggtgttca ggacttcgcc caccagctct
      301 ggatccagcg ccgacgttgg ctcgtcaaac aaaatcacct caggacgcat tgccagtgca
      361 cgcgcaatcg caacacgctg ctgttgaccg ccagacaaac gacgtggata gctggtttct
      421 ttaccggcca gcccaacttt cgccagcagc tcgcgagcgc gcgccgtggc ctcttctttc
      481 ggttcgcctt tgacgatcac cggcccttca ataatgtttt ccagcaccgt acgatgcgga
      541 aacaaattaa agttctggaa gacaaacccg acgtgctgac gcaactggcg aatcagcgat
      601 ttttgctgac ttaatgaacg tgcagtatca atagtgatat cgccaacggt gatcgttccc
      661 gcttccggtt gttccagcag atttatgcta cgtagcaatg tggttttgcc ggaaccactc
      721 ggaccgataa ttgccaccac ttcgccaggc tttacctcga ggtcgatacc gtgcagcacc
      781 gtctgaccgt ggaatttttt caccaggttc ttaacttcaa tggcactcat tttggttctc
      841 tctcctggcg attaagttga ttctcaaaat ggttctgcaa cgtcgataac acagtcgcca
      901 tgatccagta gatgagcgaa gccgccaggt acatggtgaa cacctccagc gtgcgcgagg
      961 taatcaactg cgcctgacgg aacagctccg gcacctggat tgtcgcggcc agcgacgtat
     1021 cttttaccag actgataaag ctgttcgaca gcggcggcaa cgccacccgc gccgcctgtg
     1081 gcaaaatcgc gcgacgcatg gtctgccacg gcgtcatacc aatactggca gcggcttccc
     1141 actgaccttt atcaatagaa gaaatggcgg cgcgcagcgt ttcggcggca taggcggcag
     1201 tgttcagcga cagaccaatc attgctgacg gaatgggatc taattcgata ccaaactgcg
     1261 gcaggccgta atagatcata aacagttgtg cgatgagcgg cgtaccacga aaaatagaga
     1321 tataaaaacg cgccagccag cgcacgggcc agattggcga caggcgcatc agcgcgagaa
     1381 taaacccgag tagtaagcca aaaaacatgc cgccgatact gagttgcagg gtatacccag
     1441 cccctttcaa caggaacggc aaggaatcaa taaccagttg tatactttct tgcatgagcg
     1501 ttcccggagc taaacgtggg gatgataggc gaacagcgca ggcgcgccgc cggtatgaat
     1561 aaacagaatc ggcccttcat ctttgaagcg tttctgactg ataccgtcaa tcagccccgc
     1621 catcgctttt ccggtataca caggatcaag cagaatgcct tccagacgcg ccagcaattt
     1681 cactgcttcc atgccttcgt cgttcggcac gccgtagcca ggtgcaaaat agtcatccca
     1741 gagtaaaatt tccgctgatg cggtcagctc cagttctttc gcaatcgcct gttgtaggtt
     1801 aaccactttc ggcaattgat cggcaacgga acgcgacacg gtcacgccaa tcagttcgct
     1861 ttcaggcatc aggtgttcca gcccaacagc cagtccggcg tgagttccgg cactgcccga
     1921 tgcgactacc accgacgaaa tattaaccgc cccttcacac tgttgcgcga tttccagcgc
     1981 actctccaca taacctagcg cgccaagagc attagaaccg ccaaccggaa tgacatacgg
     2041 gcgaaagcct tgtgcttcga ctcgcgtcgc cagctcttcc agttgggcat tgggatcggt
     2101 cagtgcgtcg cacatttcaa tctgggtatt gaacagatcc agcaacaaac gattgccgtt
     2161 ggttaaatag ttttctgcgg ttgtgccaat aggattttcc agcagcgcca cgcagtgcag
     2221 accgagtttc gccgcgactg cggcagtctg gcgcacatgg ttagactgga tcgcgccggc
     2281 agtaatcagc gtatcggcac cttcgcgcag ggcatctgcc gcgagaaatt ccagcttacg
     2341 taatttattg ccgcccattg ccatgggggt gacgtcatcc cgtttgatga aaatttcccg
     2401 tccaagataa tcagaaaagc gcggcagata ttcaagcggc gttggcgcgc cgataaactc
     2461 caaccgtgga aaacgggtta aattatgcag tggcataaca gcctccgatg tgtgttgttg
     2521 tgattttctt attatgcacg ctgaaaacgc gtaaataaaa aaggcgctag tgaaagcgcc
     2581 cttttttgtc attatgctga ttatttggtc acatcagcac caaaccattt ttcggaaaga
     2641 gcttgcagag tgccgtcttt ttgcatttcc gcaattgcat cattcaccgc tttcagcagg
     2701 tcttcatttc ctttacgcag cgccacgcca gactcctgac gggagaatgc ttcaccggtt
     2761 actgccagcg tatcgttggt tttcttcacc agatccagcg ccgccagacg atcaacgagg
     2821 atcgcatcga tacgccctac gcgcagatcc tgatatttgg tcgggtcatc atcataggta
     2881 cgcacatcga caccctgaac attctgccgc agccactctt catagttggt gcccagaccg
     2941 acacccactt ttttgccttt cagatcagca gccgttttaa tggtgccttc gttacctttt
     3001 ttcaccagcg cctgaatacc ggaaatggtg taaggggtag agaaatcgta ttttttcttg
     3061 cgctcgtcag aaatggtgac ctgattaatc accacatcaa tgcgtttaga gtccagcgac
     3121 gccagcatac cgtcccattt ggtcggtttt agtgacgcct caacgccaag atgttttgcc
     3181 agctgttggg caaattccac ttcaaaaccg gttaatttgc cgtcatcccc ctgaaaactg
     3241 aacggcggat aagtgccttc cagccctacc agcagcgtgc cgcgctcttt gactttatta
     3301 agcagacctt catctgcaaa acttttaacg ctcatgcccg caaccagcgc cacggccatc
     3361 acacccatca atgcctgacg tcccagatgt gctaatttca tattcacccc gaatgttgtt
     3421 atgtctgttt gcagtgtaga gccattgttt gtaaacacaa aaacaactcc gctacatctt
     3481 attcttattt aatatatatc agaagcaggc aggctggagg atttctgcac aagtccggta
     3541 caggtttggc gctgatagtg ttgatacttc cgtaatgcga tgcggtaatt gttggtgctg
     3601 gtggtgggca gccacggggc gaggatttca tcaaggaaac cgtcctgcag ctgatcgagg
     3661 gaaatatttt gctcatgtag atgattaccc aaacggcgca gacgaacgac atattcacgc
     3721 accgtacctg ggctcatttc agtttgctca aaaagaaatt tcttaaagcc gataatatcg
     3781 aaaaaatcac tctgcgtttt gcaatgtaaa tcaccgcaaa atcggcacaa tgccgcccat
     3841 gattgttgtt ccgtttgcca gcctttttca tcaagcagcg tgtccaggcg ggaaatctcg
     3901 attttattca ctattttgcc gtcgcgaact aaggtaatgc gatcgagtaa tttacggcaa
     3961 tgcgcgcaat gggtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
     4021 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa agttaaagtg cggcatttac
     4081 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
     4141 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
     4201 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
     4261 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
     4321 tcagtaacca gttcgatgct atcgccgtgc tcttcacgcc actcatcgta ggagaagagc
     4381 tggctgttat tggtgtcgag cagcatttga cgataatcgg caatatcgat ccctaaacgt
     4441 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
     4501 tgtgccactt cacgctcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
     4561 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
     4621 agggcgtcat agcgttcgac agcattaagt aacccaatgc cgcccgcctg tagcagatcg
     4681 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     4741 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     4801 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggg
     4861 tttttaatcg gacgattagt gggtgaaatg aggggttatt tgggggttac gcaaaaagcc
     4921 gaatgtaaaa aaccccgccg gtggcggggt ttgagaggca ttcagacgat taaccctgca
     4981 gcagagacag aacctgctgc ggtacctggt tggcttttgc cagcacggag ttaccggcct
     5041 gctggataat ctgcgctttc gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa
     5101 tacgggactg cgcttcagac aggttggtag tggtgttgtt caggttggtg actgcggaat
     5161 ccaaacggtt ttgcaccgca cccagggagg agcggaattt gtcgatggag ctgatggctt
     5221 catccagagc tttcagggga tcggctgtcg tagctgcttt tgtttcagca tctgtcgtca
     5281 atttaccatc agcatcttta taaactgcct taccgctgcc gttagtcact gaaccatcat
     5341 cctgtaggta aaaatctaca gtatcagatg tggtcaacgc cccgttgtta tcaacataaa
     5401 ccgaatcaac accttttgtt acagcgccag ttgtgccgtt gacgctataa cctgcagtag
     5461 tgaaatcaac attgtttccc gaggttgata cagctttatc gaaagctgcc tgatcaactt
     5521 ttgcacctgt tactgaataa gtaatagtgt ccggcgtagt actattaccg gtaaaggaga
     5581 tagaggtacc gccaatgtca atggttgtcg ctttggtatt attggctgca agtgtggaca
     5641 gactagcctc agtcaaagaa gcaccagagc cgtttttcgt taagcgccca gttgtatcaa
     5701 tgtagagttt atctccattg cttgccgtaa ttttaccatc actgctgatc tgaacatcct
     5761 gtgcagaacc atcgatagta atagtgccct tagcggtatc accaactccc ggggtcaaat
     5821 atttctgaac atcggcagct gaagccgttg tggtatcaaa agagtatgac ttacttgcac
     5881 tgtcatactt gtaattcgtt
//
LOCUS       DADKXM010000184         5896 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.187, whole genome shotgun sequence.
ACCESSION   DADKXM010000184 DADKXM010000000
VERSION     DADKXM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.187"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            455..2734
                     /gene="ydeP"
                     /locus_tag="JQR05_003708"
     CDS             455..2734
                     /gene="ydeP"
                     /locus_tag="JQR05_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY5325728.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2982..3179
                     /gene="safA"
                     /locus_tag="JQR05_003709"
     CDS             2982..3179
                     /gene="safA"
                     /locus_tag="JQR05_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY5325729.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3253..4014
                     /gene="ydeO"
                     /locus_tag="JQR05_003710"
     CDS             3253..4014
                     /gene="ydeO"
                     /locus_tag="JQR05_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY5325730.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSVLAI"
     gene            4416..>5896
                     /locus_tag="JQR05_003711"
     CDS             4416..>5896
                     /locus_tag="JQR05_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY5325731.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLV"
BASE COUNT         1706 a         1283 c         1279 g         1628 t
ORIGIN      
        1 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
       61 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
      121 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
      181 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      241 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      301 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      361 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      421 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      481 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      541 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      601 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      661 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      721 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      781 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      841 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      901 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      961 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
     1021 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1081 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1141 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1201 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1261 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1321 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1381 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1441 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1501 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1561 cactcagcac gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa
     1621 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1681 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1741 tgagcgctat ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1801 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1861 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1921 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1981 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     2041 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2101 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     2161 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     2221 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     2281 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2341 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2401 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2461 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2521 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2581 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2641 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2701 aagtattccg gttgaattag aaccatcaaa ttaatgcctc ttctcatttc ttctgctgtc
     2761 atccgcacag cagaagaatt cctcattgac tattattgcg caatttgctc acatggatta
     2821 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2881 gtactgaagc accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat
     2941 taacgacttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     3001 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgcaatt gtggttgtct
     3061 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     3121 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3181 aataaacagc aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt
     3241 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct
     3301 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3361 gttcgtacac attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
     3421 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3481 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3541 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3601 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3661 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3721 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
     3781 acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3841 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3901 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3961 caaaggcaca cgggtatgaa tacgggcaac acgatgagtg ttttagctat ttgattattt
     4021 gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc
     4081 cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt
     4141 tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt
     4201 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4261 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4321 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4381 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4441 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4501 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4561 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4621 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4681 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4741 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4801 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4861 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4921 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4981 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     5041 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5101 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5161 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5221 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5281 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5341 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5401 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5461 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5521 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5581 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5641 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5701 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5761 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5821 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5881 attattcgct tgtcta
//
LOCUS       DADKXM010000185         5827 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.188, whole genome shotgun sequence.
ACCESSION   DADKXM010000185 DADKXM010000000
VERSION     DADKXM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.188"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..483)
                     /gene="ynaI"
                     /locus_tag="JQR05_003712"
                     /pseudo
     CDS             complement(1..483)
                     /gene="ynaI"
                     /locus_tag="JQR05_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
     gene            727..984
                     /locus_tag="JQR05_003713"
                     /pseudo
     CDS             727..984
                     /locus_tag="JQR05_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(1034..1984)
                     /gene="uspE"
                     /locus_tag="JQR05_003714"
     CDS             complement(1034..1984)
                     /gene="uspE"
                     /locus_tag="JQR05_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY5325732.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(2136..2888)
                     /locus_tag="JQR05_003715"
     CDS             complement(2136..2888)
                     /locus_tag="JQR05_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY5325733.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(3083..3598)
                     /gene="ogt"
                     /locus_tag="JQR05_003716"
     CDS             complement(3083..3598)
                     /gene="ogt"
                     /locus_tag="JQR05_003716"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY5325734.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3609..4274)
                     /locus_tag="JQR05_003717"
                     /pseudo
     CDS             complement(3609..4274)
                     /locus_tag="JQR05_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            4371..5399
                     /locus_tag="JQR05_003718"
     CDS             4371..5399
                     /locus_tag="JQR05_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5325735.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            5396..>5827
                     /locus_tag="JQR05_003719"
     CDS             5396..>5827
                     /locus_tag="JQR05_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY5325736.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLS"
BASE COUNT         1544 a         1310 c         1387 g         1586 t
ORIGIN      
        1 tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag
       61 gctcatgccg aaatgttcgc cataaagcag aacaagcact acaataatgg tgattttgat
      121 aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc
      181 aaactgatta atcagcagaa atagcttaat cgtcagaata accgcaatca gggacgtaca
      241 gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt
      301 aatgatgcta ccaacagcac tgataattat ggtttagatt aaaaattgca ccgcatggaa
      361 taaaaatcct tttcttttac gatttccacg gcgaaaccaa aagctcatca gaatcaatgc
      421 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
      481 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaacccc tggcagggct
      541 attttaccgc gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag
      601 ctaactaatc aatgcgttga ttgtaaatcc agctaagagg tgaggttttc agagcagaca
      661 acggtgaaac gtcatggtat tgttacgttt aggtaacaag aaatttgtct gcacaaggat
      721 tacatcatga ttatggcgaa actgaagtca gcgaaaggga agaaatttct ctttggtttg
      781 ttggcggttt tcattattgc ggcgtcggtt gtgactcgcg caaccatcgg cggcgttata
      841 gaacagtaca atattccgct gtctgagtgg acgacatcaa tgtatgtgat ttagtcatcg
      901 atgatttttg tttatagcct ggtctttact gtgttgctgg caatcccgtt gggaatttat
      961 ttccttggcg gcgaagagca gtaagtaaaa aataggcccg ataactcggg ccttgtcagt
     1021 tattgaagag tcgttaatcg tcttcttcgt catccagttc aacgggtgtc tgatactggt
     1081 caggtttaat gaccagcagg tcgcagcgaa gatgatcaat cacctgttcc gccgtgttgc
     1141 cgaggaatgc tgctgaaata ccggtgcgtc cgaccgtgcc cagaaccaca attcccgcct
     1201 gtaaatgctc cgccaaatca ggaatcacct cttcgggcag acctttttct acgtgcgtca
     1261 tgttttcatt aatgccgaat ttctgccgca gggctttcat tgccagcaaa tgttgcccac
     1321 gaatggcatc gttataaacg ctcgggtcaa attccggcag ttcaatcgcg atattaattg
     1381 gcgttaccgg ataagcgcca accagatgaa cttcggtatg gttgacttgt tctgccagtt
     1441 cgatcgtctc tttgaccagt ttttcattga gcgcattatg atacggctct tcactggcga
     1501 gattcaccgc caccagcgcc ttgcctcctt ccggccacgg ctggtctttc accatccaca
     1561 ccgggcttgg gcatttgcgt aacagatgcc agtccgttgg cgtaaaaatc accgcttcca
     1621 gacggtcatg ttggtgcgcc atttttagca ccaaatcgtg tccgccgctg atcacttcct
     1681 gaatgatggc ttcgaaagga cggttatgcc agaccacttt aatttcaatg ggaacgccag
     1741 cattgagata atattttgcc tgctcgtgga tccaggctgt acgctggctg atgacgccct
     1801 gacgcatagc ggtacgttcg tccggggaga gcagggtggt catttcgtat gagaagtcat
     1861 agatcggcaa aaaggcttta attttgccac caatccgttg atgtaaataa acagctcgcc
     1921 gcaatgctgg ttggtcgtcc tggttaggat cgataacaac gagcatgttc tgatacatag
     1981 ccatacaggg tctccttaca acaactgtca acgcagtttg taattaaaag attaacccat
     2041 atctggtgaa tgaaacagtg atgaaccttc tgccagatca ataaatcaga aaaatttaat
     2101 gatatgacag aaggatagtg agttatgcgg aaaaatcagg caacgttacg cgtatgacca
     2161 gcaagctggg ccagcgcatc gttattttcg atggtgatgt atttaccttt gactgccagc
     2221 ataccgcttt tctggaagcg ccccagcaga cggctgatgg tttctaccgt caggcccaga
     2281 tagttaccaa tatcgccacg agtcatcgtc aggcggaatt cacgagggga gaagccgcgt
     2341 tgggcaaaac gacgggacag gttgtagatg aatgcagcca gacgttcctc ggcatttttc
     2401 ttcgacaaca gcaagatcat gtcctgatcg cctttgattt caccgctcat cagacgcatc
     2461 atctgctgac gcagattcgg cattttaccg gacaaatcgt ccagcgtttc gaacggaatt
     2521 tcacatacca tcgaggtttc cagcgcctga gcgaagctcg ggtgatggcc gctgccgatg
     2581 gcgtcaaatc ccactaggtc gcctgctaaa tggaaaccag tgatttgctc gtcgccttgc
     2641 tcagtgattg tataactttt aatcgtaccg gagcggatgg cataaagcga tttaagttca
     2701 tcaccagcct taaacagcgt ctggcctttc tgaataggct tcttccgctc aatgatatta
     2761 tcaagctgat caagctcatg ttcgttgagt gtgaacggga tgcaaagctg gctgatgctg
     2821 caatcctggc aatggatagc acaaccgcca gactgaatgc gccgtataat tcgcttttcc
     2881 gggatcatag gtctgctcaa gccgtaattg atatttgtca attttaacat ctttttaggg
     2941 agcaagtaag tctaagcaaa ccttaacagc agagaattcc gatattagat gtaaatatat
     3001 gtctatctat ttgaaactcc ttaagttgtc aagggtaact ttacataaaa gtgtgaacaa
     3061 gctggcacaa attgtttaat gtttacagca aaagataacc ttcgtggcgc aataaccact
     3121 cttttcgctg aactccgcct gcatatccgg tcatggtgcc gtttcggcca ataacccgat
     3181 ggcaaggtac gacgatgctg atgggattcg atccgtttgc cgcaccaacg gcacgcgccg
     3241 cgccagggcg gcccaattgt tcagccagtt ggccgtaatg cattacctgc ccgcagggga
     3301 tagtgcgtag tgttttccag acttcgcgct gaaatggcgt cccccccgtg gcagtgggaa
     3361 gcgtatcaat aatgctaaga ttaccggcaa aatattcacg aagcttgtcg cttaaaccac
     3421 ctgggttggt ggcagaaatg cgctcatagc cttctttgcg ataatggatg tccagcagct
     3481 gcaccatgcg ttcgctgtac tcttcccatt caaccgcccg caggcgaaat tgctcatcgc
     3541 aaatcaccca cagtggaccc agtggcgtgg caattttttc ttcaagtaat ctcagcatcc
     3601 gttctctctc catcatcaag aagcgtcact gcagaaataa cgtgcatttg ctgaacccag
     3661 tggcgaaatg ttaatcccgt ctctttaaca aataagcgca tgagtgtacg gctactgacg
     3721 ttaattttat ccgctagatt atccagtgtt ggcagatacc cttggttgtt tagcaaattc
     3781 tcacaaagct gctgtagccg ctcatcattt ggccaaggga gttcaagtga cattttcacc
     3841 cccattttta tttcctgaag tatcagtgtc tcgagggctg aaaaacgtaa ctgttgttta
     3901 tctgtggtac tttgcgacgg tggaagttgg ttaattgcaa ttagtaattc ccttaacagg
     3961 ggtgaaatac ccgtcatgaa gcaaactgtt cccagcgttt cactttccgc ttctgaaacc
     4021 agtacggttt ttaaaagtac gttgcctgtc gcaacaaact tatgcggcat ttgtgggggc
     4081 agccaaagcg cgctgctggc atggactagc cagacgcttt ctccagccca tacacgaacg
     4141 actccttcag ccgggtaaag caactgggca tagagatgtt tatgccaggg aactgtacct
     4201 ccattcagat gttccgaatc cagtccgata atgtgatgcc ctggtgatgc tttgggttgc
     4261 agtgtaattg tcatttacag taggtatttt gtcagaaatc atcaaataag ccatgttaaa
     4321 ttttaaatat gacgtatgca agtttccgga tatatacggt gtaaagagtg atgataattt
     4381 ctaaaaaaca gcttattggg gtggtagcga tcggtattct tttgactggc gttgtctttt
     4441 ttatctggag ggtaagcaaa ggaagattta tacagacgac ggatgatgct tacattggtg
     4501 gtaatatcac cacggtggca agtaaagttt ctggttatat ttctgctata gaggtgcgcg
     4561 ataatcagag cgtaaaaaaa ggcgatatta tcctgcgtct cgatgatcgg gattatcgtg
     4621 caaatgtcgc caggcttgag gcaaaaataa aaagtagcaa agccaatctc gaaggcattc
     4681 aagccactat tacgatgcag cagtcgataa ttcaaagtgc cagtgaaaca tggcaggcag
     4741 taaaacatga ggagcaaaag cgcctgcgag atactgaacg ttatgaaaaa ctcgcacaat
     4801 cagcggcaat atcgcaacaa attattgata acgccaggtt tgactaccag caggtcgcag
     4861 caaaggagcg caaagccgcg aacgatttcc tggtggagaa gcaacggctg gcagttttat
     4921 ctgctcagga agaaaacgta cgtgcgagta tcgaagaagt tcaggccgct ttgactcagg
     4981 cactactgga tctggaatat acccttgttc gtgcgccgat tgatggcatt gtcgcaaacc
     5041 gcagcgccca tacaggcagt tgggtggaag gcggcacatc gttagtttca ctagtaccag
     5101 tcagtgagtt gtgggttgat gcaaattata aagaaaacca aatagcggga atgaagccag
     5161 gaatgaaggc tgagatccgt gctgatattc tcaaagggga agtgtttcac ggtcacattg
     5221 aaagcctttc cccagcgaca ggagccagtt tcagcttgat tccgatagaa aatgccactg
     5281 gcaatttcac caaaattgtt cagcgtgtcc cggtacggat tgcatttgac gacgcaaagg
     5341 aattaaagca acttcttcgc ccgggactgt ccgtcaccgt cagtgttgat gagagatgat
     5401 gtctatgcgt aaacatattg ctttcgccag tatgtgtatg ggattattta ttgctcagct
     5461 tgatattcaa attgtatcgt catccctaaa tgaaataggt ggtggtctgt ccgcaggtaa
     5521 agacgaaatg gcctggcttc agaccagcta tttaattgct gagattattg ttattccgct
     5581 ctctgggtgg ctgtcacggg tattttccac tcgttggctt tttactctat ccgcgggaat
     5641 atttacttta atgagtatcg cgtgtggact tgcatggaat attcagatca tgattttgtt
     5701 tcgtgcttta cagggagctg ctggcgcatc catgatccct ttagttttca cgacggcgtt
     5761 tatttattac cagggtaaag agttggggtt ggctgctgcg gtagtcagtg cgctggcctc
     5821 actgtca
//
LOCUS       DADKXM010000186         5778 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.189, whole genome shotgun sequence.
ACCESSION   DADKXM010000186 DADKXM010000000
VERSION     DADKXM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.189"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(220..2289)
                     /gene="glyS"
                     /locus_tag="JQR05_003720"
     CDS             complement(220..2289)
                     /gene="glyS"
                     /locus_tag="JQR05_003720"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY5325737.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2299..3210)
                     /gene="glyQ"
                     /locus_tag="JQR05_003721"
     CDS             complement(2299..3210)
                     /gene="glyQ"
                     /locus_tag="JQR05_003721"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY5325738.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3305..3604)
                     /locus_tag="JQR05_003722"
     CDS             complement(3305..3604)
                     /locus_tag="JQR05_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325739.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3779..4774
                     /locus_tag="JQR05_003723"
     CDS             3779..4774
                     /locus_tag="JQR05_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY5325740.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4816..5253)
                     /locus_tag="JQR05_003724"
     CDS             complement(4816..5253)
                     /locus_tag="JQR05_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY5325741.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5299..5640)
                     /locus_tag="JQR05_003725"
     CDS             complement(5299..5640)
                     /locus_tag="JQR05_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325742.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1430 a         1463 c         1395 g         1490 t
ORIGIN      
        1 ctgcctttcg gtgaatacct gagacaaaca gtctcaagca cccgtggcta ttctagctta
       61 ataagtttgt ttcttctcct tgatataatc ctaaaaaaat ctcataaaat taatatatga
      121 gataatcttt attcagcaga agattattaa aggttgctgt attatttagc gataataaaa
      181 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
      241 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      301 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      361 ctctgccagt tcgaccagcg catcctggta acgaccttcc gcaaagtacg gctccagctt
      421 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      481 ggcattcacg cgatcactca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      541 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      601 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      661 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      721 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cctcttcggt
      781 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      841 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      901 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      961 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
     1021 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
     1081 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
     1141 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1201 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1261 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1321 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1381 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1441 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1501 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1561 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1621 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1681 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1741 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1801 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1861 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1921 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1981 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     2041 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     2101 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     2161 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2221 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2281 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2341 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2401 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2461 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2521 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2581 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2641 cagcgggccg tcgctccaga ccaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2701 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2761 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2821 tccccaggcc cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2881 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2941 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     3001 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     3061 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     3121 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3181 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3241 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3301 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3361 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3421 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3481 cttccatatt caacgaacgc agaggatcaa cctttacccg ttgtgctttc tgcacgggtg
     3541 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3601 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3661 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3721 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3781 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3841 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3901 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3961 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     4021 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     4081 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     4141 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4201 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4261 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4321 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4381 agcactgtcg tgggtgagcg ccgcgctgtt tgcgactggg gtttttatta tctcacgcgg
     4441 gacattatat gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg
     4501 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4561 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4621 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4681 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4741 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4801 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4861 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4921 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4981 catcagtagt gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     5041 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     5101 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     5161 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5221 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5281 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5341 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5401 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5461 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5521 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5581 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5641 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5701 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5761 atatatagat gtgaatta
//
LOCUS       DADKXM010000187         5751 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.190, whole genome shotgun sequence.
ACCESSION   DADKXM010000187 DADKXM010000000
VERSION     DADKXM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.190"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JQR05_003726"
     CDS             complement(<1..207)
                     /locus_tag="JQR05_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325743.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="JQR05_003727"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="JQR05_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY5325744.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="JQR05_003728"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="JQR05_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY5325745.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="JQR05_003729"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="JQR05_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY5325746.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDTEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="JQR05_003730"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="JQR05_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="JQR05_003731"
     CDS             complement(3279..3398)
                     /locus_tag="JQR05_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY5325747.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="JQR05_003732"
     CDS             complement(3359..3544)
                     /locus_tag="JQR05_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325748.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="JQR05_003733"
     CDS             3645..3818
                     /locus_tag="JQR05_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325749.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3880..4164
                     /locus_tag="JQR05_003734"
     CDS             3880..4164
                     /locus_tag="JQR05_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY5325750.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="JQR05_003735"
     CDS             4168..4503
                     /locus_tag="JQR05_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY5325751.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..>5751)
                     /locus_tag="JQR05_003736"
     CDS             complement(4560..>5751)
                     /locus_tag="JQR05_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5325752.1"
                     /translation="GIEIISVEGESNGEFIKDSRIFAGAYEYSLTRGNTEATNKNWYL
                     TNFQATSGGETNSGGSSAPTVAPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGETRY
                     IDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGGDLLTGGFTDSDSWR
                     LGVMAGYARDYNLTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDSWAQYSW
                     FKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIWMGV
                     DHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIHNSK
                     DFAVSMNGVKVEQDGVSNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVGL
                     KYKF"
BASE COUNT         1569 a         1367 c         1332 g         1483 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcatca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacatc ttctgcggtt tctatgatag agcaattcta tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt tgacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat acagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agaatcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc a
//
LOCUS       DADKXM010000188         5711 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.191, whole genome shotgun sequence.
ACCESSION   DADKXM010000188 DADKXM010000000
VERSION     DADKXM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.191"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1537)
                     /locus_tag="JQR05_003737"
     CDS             complement(<1..1537)
                     /locus_tag="JQR05_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY5325753.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPMQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHFSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAM"
     gene            complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JQR05_003738"
     CDS             complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JQR05_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY5325754.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JQR05_003739"
     CDS             complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JQR05_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY5325755.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JQR05_003740"
     CDS             complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JQR05_003740"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY5325756.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4828..5592)
                     /locus_tag="JQR05_003741"
     CDS             complement(4828..5592)
                     /locus_tag="JQR05_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5325757.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1447 a         1507 c         1347 g         1410 t
ORIGIN      
        1 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
       61 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
      121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
      181 aacattcagc aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
      241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
      301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
      361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
      421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
      481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
      541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
      601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
      661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
      721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
      781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
      841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
      901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
      961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
     1021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
     1081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
     1141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg ccatacagca
     1201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
     1261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
     1321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
     1381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
     1441 tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac ggaaaaattc
     1501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
     1561 gtgtgcaact ttgttgggcg gttgtctggt gggaattttg cgttccccga ggcacattca
     1621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
     1681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
     1741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
     1801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
     1861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
     1921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
     1981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
     2041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
     2101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
     2161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
     2221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
     2281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
     2341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
     2401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
     2461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
     2521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
     2581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
     2641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
     2701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
     2761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
     2821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
     2881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
     2941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
     3001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
     3061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
     3121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
     3181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
     3241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
     3301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
     3361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
     3421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
     3481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
     3541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
     3601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
     3661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
     3721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
     3781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
     3841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
     3901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
     3961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
     4021 acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
     4081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
     4141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
     4201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
     4261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
     4321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
     4381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
     4441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
     4501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
     4561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
     4621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
     4681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
     4741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
     4801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
     4861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
     4921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
     4981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
     5041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
     5101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
     5161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
     5221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
     5281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
     5341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
     5401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
     5461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
     5521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
     5581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
     5641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
     5701 ccgacaatgt g
//
LOCUS       DADKXM010000189         5702 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.192, whole genome shotgun sequence.
ACCESSION   DADKXM010000189 DADKXM010000000
VERSION     DADKXM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.192"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..1329
                     /locus_tag="JQR05_003742"
                     /pseudo
     CDS             1..1329
                     /locus_tag="JQR05_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            1512..3692
                     /locus_tag="JQR05_003743"
     CDS             1512..3692
                     /locus_tag="JQR05_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY5325758.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(3833..5116)
                     /locus_tag="JQR05_003744"
     CDS             complement(3833..5116)
                     /locus_tag="JQR05_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY5325759.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5251..>5702)
                     /locus_tag="JQR05_003745"
     CDS             complement(5251..>5702)
                     /locus_tag="JQR05_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5325760.1"
                     /translation="VTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDAL
                     GISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHE
                     LRSLSARLYEKQISDKFSQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1353 a         1390 c         1479 g         1480 t
ORIGIN      
        1 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
       61 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
      121 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
      181 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacgtat tgcctatctg
      241 ctgattggta aaatcggtaa caccacgctg ggtctgggtt acgttacggt attcctcgat
      301 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
      361 atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
      421 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
      481 accgcaatgg aggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
      541 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
      601 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
      661 atcgctaaag cgggccttgc agaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
      721 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
      781 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
      841 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
      901 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
      961 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     1021 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     1081 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     1141 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     1201 ggcgtgggtt acaacgatat taaatcctga tggttggtcg gtgcggtact gacgatatta
     1261 accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     1321 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     1381 ccagtcactt acttatgtaa gctcttgggg attcactcac ttgtcgcctt cctgcaactc
     1441 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     1501 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     1561 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     1621 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     1681 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     1741 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     1801 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     1861 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     1921 tgatggcagg cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     1981 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     2041 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     2101 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     2161 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     2221 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     2281 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     2341 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     2401 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     2461 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     2521 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     2581 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     2641 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     2701 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     2761 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     2821 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     2881 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     2941 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     3001 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     3061 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     3121 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     3181 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     3241 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     3301 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     3361 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     3421 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     3481 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     3541 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     3601 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     3661 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     3721 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     3781 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     3841 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     3901 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     3961 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     4021 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     4081 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     4141 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     4201 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     4261 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     4321 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     4381 taacgttgtt aatctgtacc tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     4441 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     4501 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     4561 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     4621 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     4681 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     4741 ccgttccctg atactcacca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     4801 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     4861 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     4921 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     4981 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     5041 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     5101 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     5161 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     5221 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     5281 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     5341 agaaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     5401 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     5461 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
     5521 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
     5581 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
     5641 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
     5701 aa
//
LOCUS       DADKXM010000190         5695 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.193, whole genome shotgun sequence.
ACCESSION   DADKXM010000190 DADKXM010000000
VERSION     DADKXM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.193"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..266)
                     /locus_tag="JQR05_003746"
                     /pseudo
     CDS             complement(1..266)
                     /locus_tag="JQR05_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(452..3760)
                     /gene="icsA"
                     /locus_tag="JQR05_003747"
                     /gene_synonym="virG"
     CDS             complement(452..3760)
                     /gene="icsA"
                     /locus_tag="JQR05_003747"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY5325761.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINTDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSMINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKDRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4289..5491
                     /gene="virA"
                     /locus_tag="JQR05_003748"
     CDS             4289..5491
                     /gene="virA"
                     /locus_tag="JQR05_003748"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY5325762.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1775 a         1287 c          937 g         1696 t
ORIGIN      
        1 atttatacct actctgacca agtctggata agaaaggcac acctgcacat ttctacttgg
       61 aaatgttttc ttttaccatt ttgctcaaaa atttcaacat ccaatattcc tcgtacagaa
      121 ctactcaagt cctgaataga aaaagttcca gctggtacag tagagtcata tattactcga
      181 ccttgttgag atacaacaac acgtgcatta gtttctgcaa taccagtaat ctgaggcgca
      241 taaccacgaa tatttggcgg tagcattcgt tcatcgcttt ctaatgcaat tcctgtgtaa
      301 cgccaagcaa cagcgcattc tggcgcatga tctggtaaac acgtttggca ttgatcgcag
      361 gcataccatc atgtgcacaa aaacccaccg agcttttaca cggtgggtcc cagagaaatg
      421 caggacatca acacgccctg catttttatt atcagaaggt atatttcaca cccaaaatac
      481 cttgggtgtc tctgtaactg ttattaccca attgctgaga tacgtttccc cagatgctta
      541 ggttatctgc aagacgcccc cccacaccag ttttgagttc aatcacatta cggttgccta
      601 tctggtgatt tgacatgcca ttcataatta caccatattg ctttgagctc catttccaat
      661 tcacttccaa aaatggttcg aacttacgaa gagtctcctt atcgatacaa ctctgactaa
      721 ttaaggatgt acgcaatccc aattttgctt gaatattatt gaccccagag gatgtgacca
      781 tagtgccatt gtgttcaata tagtcgtttg cttttacccc atgcctagtt atctggactt
      841 gtggattgag atacaatgcg ttgtcaatat tattatgagc agtccagcgt atggttgggt
      901 agatatagcc agcttccaaa gctcctgtta ttcctttgct ggaatatttc tcaccagtta
      961 acccatctcc tttgactgta ttattaaacc aaccgtactg caaccaagca tccatatata
     1021 gacctgttcg ttctttttca tcctgaaacc aagaactgta caaaccggca gtataaccgc
     1081 tcacagttcc tcgtgaacta taactagtat acttattatg agttttgcta tcctgatatc
     1141 cataagctcc cataataccc acatgcaagc catcatgatc agtgaagttt gtcttcaaaa
     1201 tatctccacc aatctgataa accatcctgt tagctgtggt ttttaattgt ctatcagcta
     1261 aattatgctc attatgtcca ccaacagtac gaatccacat ggtggtttcg ctgagctgac
     1321 ctgtagtagg atcaacaata gtgctaccgt gtcggtcata gagagaagga ctaaacatcg
     1381 tgttcattgc tatcaggtta gcaatataac tgccagcttc agggcgccat gtgtgaatac
     1441 taccttcacc caaattaagt gaagatacaa gctgtgaact agctcggcga ctactcattt
     1501 gagtagactc ttgattgctc atctgttttg attcttgatt atccatctga ctagttagat
     1561 accacttatt ggtattcagt ccagatacag tgccctgttt caggcgatag tcataactac
     1621 cagcaacaat acggtctttc tgaataaaag cattcttatc agaagaatct gtcgaaataa
     1681 tttgaacacc ttcaagagtt ttatctccag agcctccctc atttgtcact gcaacatagg
     1741 taataccaga cgcatggcct gcaatttcca gccggtcagt tttactgttg tcattgccga
     1801 gaaccgcgcc caaagataat attccgccct ttccatgcca attacctttc tgaacatagg
     1861 tctgaccgac atttgacgaa ctattattga gaataacatg accgtttttc tccagagtca
     1921 tgttacctgt gacaatgacg gggtcaggca aaaaaggggc attaatatta ttgattagca
     1981 cagtcccact atttaataaa gtgttaacag tttggttcat gcctgaaaaa ttcaaggtag
     2041 cacctttatt aacaataaca ccagcggtac gtgtcatagc ttcaactgtc cccattttta
     2101 gaatacctgc attgatgttg gtgaaaccag agtaggtatt tttttccgcc aaaatgagag
     2161 ttccctcccc tgattttgtt agggattttc cgtcccagct atttatatta tgatggctga
     2221 gattatctgc cagaatagta ctaacagtaa gttcagcgtt ttctttcaga ttgaactctc
     2281 catgactgtc gccatctgta tcattccagc tcaaaccgta gatcaattta ttctggtcac
     2341 tgatatagct ttttgtatag tcaatagcac cagtagtgag taacggattg atgatagata
     2401 tattagaaac actgaaacct gttatccctt tatctgcact tagcagttta tagtccttct
     2461 gcgcccagtt atttataata gcttccactt ttacattgtt gccaaatgac atatttccag
     2521 aagcttccag actactgatt ccagctaaat ttaaagaact gttctcacca aaagaaacgt
     2581 cacctgaaac agtgaatttg ttacctgata gtgaaacagt tgtacaatca ccagcacata
     2641 aatcaccttc aatagttggt gtggtcccag tgtaatcatt gttgataatt ttcagaatgg
     2701 atgaattgtt aacctgtaca tcaccagtaa tgacagaacc ttcatggagt attaatgaat
     2761 tgtttccacc agtgatgtgg atagcattac ctgctacatt ttgtgttcca tcatcttctt
     2821 tacctgaaat tatatatccg ctattagcaa tggtcatatt gctaccattt atagcatccc
     2881 cccaatgtcc accattaccg cctaaaatag ctccattgtt gatcatagac aggttatctc
     2941 ctgtgatagc attaccaccg tagccatcat aaccataacc gtaagcagca cctccgttgc
     3001 ctccagcgaa cgtgccctta ttgattacag agaggctgct tcctgtgatg gcatcacctc
     3061 cattaccgcc atcaccctca ccataattgt tacctccatt accaccggaa atagtccctt
     3121 tgttgattat gggtagattg actcctgtga cggcatcacc tccattcccc ccatcactat
     3181 cactataact atcgccacca tgtccacctg aaataatttc tccatttatt ataaacagat
     3241 tgtctcctgt aacagcctca ccaccatcac cgttatggtc agcaccgcta ccgccgctac
     3301 caccaagaat catgccttga ttgattatag acaagtcaga tccggtaata gagtcaccac
     3361 cattaccgcc acaactatta ccatcattat tatcaccagc accaccatga cctgcgctaa
     3421 tcataatatc agtgttaatc cgtagcttag ttctagatgc atgagagggg ttggaagaag
     3481 ttatatacca cgcatccatt ccatcttcac cagctcccaa gggagaaagt ccatcaacag
     3541 gtgttaataa tttttcataa ttgtcctctg aaaaatgaag ttcttgcgta cccgaaagag
     3601 gaatagcaaa agctattggc cccccgagca acagggatgc accaacaaca aaaggagaaa
     3661 aagacaattt gcatgttttt tcctttaccg taggtaattc tccggccccc ccctgtgaac
     3721 attgggtcat attacaaaaa aatttgtgaa tttgattcat gcactatatt atcagtaagt
     3781 ggttgataaa cccctaaaaa gattccgttt tatccggaat aaagggacga tatatgcaaa
     3841 aacatattaa acaaagcctc aaccaaaaca tgagagctgt gttatatatc aatgcaacgg
     3901 gagaaattta cctgtgtctt gctttgaaaa aaagaggggg aaagctatat gttcgataat
     3961 aaattaataa ttttactatg atagtctgct tcagaccgca acttttcagt tctttctgta
     4021 cgcttgccca aagtttaacc actttgtttt tctatacacc caatggttct atctgcccct
     4081 tgatgttaat ccccaaagcc aataacatga atgtattcat atcacaactt tcctaccaga
     4141 ctctccaatg caataactgc ataattgttt ttagtggaaa tgtatcgttt tcttaaagag
     4201 aagaataaca ttccacttta ttatgttccc aatttaatta tattaatata tattggcatt
     4261 aatagaaaaa tacatcagga gaaatcaaat gcagacatca aacataacta accatgaaag
     4321 aaatgactca tcatggatgt caactgtcaa atcaactaca gaagtaagtt ggaataaatt
     4381 gagtttttgt gacgttttac tgaaaataat aactttcggc atatattctc cacacgaaac
     4441 tctagccgaa aaatatagcg aaaaaaaact aatggatagt ttctcaccat cactatcaca
     4501 agataaaatg gacggagagt ttgcccacgc aaacatagat ggcatttcaa tacggctatg
     4561 ccttaacaaa ggcatatgtt cggtttttta tcttgatgga gataaaatac aatccactca
     4621 actttcatca aaagaatata ataacttgtt aagttcactc ccccccaaac aattcaactt
     4681 aggaaaagta catactatta cagctccggt atcagggaat tttaaaactc acaagcctgc
     4741 accagaagtt attgaaacag ccattaattg ctgtacctcg ataataccaa acgatgatta
     4801 cttccccgtt aaagacactg attttaatag tgtttggcat gatatttaca gagatattcg
     4861 agcatctgac agcaatagta caaaaatata tttcaacaat attgaaatac cactaaaatt
     4921 aattgcagat ctaattaatg agttaggaat taatgaattc attgatagca aaaaagagct
     4981 tcaaatgcta agttacaacc aagtaaataa aataatcaat tcaaacttcc cccaacaaga
     5041 cctatgtttt caaacagaaa aacttttgtt tacttcactc tttcaagacc ctgcatttat
     5101 atctgcacta acatctgcat tctggcaatc tcttcacatc acgtcttcct ctgtggaaca
     5161 catatatgcc caaattatgt ccgaaaacat agaaaacagg cttaatttca tgcctgaaca
     5221 acgagttatt aacaattgtg gacatattat aaaaatcaac gcagttgtcc ctaaaaacga
     5281 cactgcaatc tctgcctctg gggggagggc ttacgaagtt agctcatcaa tattaccatc
     5341 gcacataaca tgtaatggtg ttggtattaa taaaatagaa acaagctatc tcgttcatgc
     5401 tggaacactt ccaagcagtg aagggcttcg caatgctatc cctccagaaa gtcgtcaagt
     5461 atcatttgcc atcatatctc ctgatgttta agccaatatt atattggctg ccagactgta
     5521 aaaaaacgtt cttcagaaca gctctataaa ctattaaacc aaagctaaaa ggatggggac
     5581 caatagaatc tcagcccatc ctttttatat cattatgaac cgccccggaa atcctggaga
     5641 ctaaactccc tgagaaagag gtaaacagga tgactaaaaa tacacgtttt tcccc
//
LOCUS       DADKXM010000191         5687 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.194, whole genome shotgun sequence.
ACCESSION   DADKXM010000191 DADKXM010000000
VERSION     DADKXM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.194"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(206..281)
                     /locus_tag="JQR05_003749"
     tRNA            complement(206..281)
                     /locus_tag="JQR05_003749"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Asn,seq:gtt)
     gene            complement(382..2025)
                     /gene="yeeO"
                     /locus_tag="JQR05_003750"
     CDS             complement(382..2025)
                     /gene="yeeO"
                     /locus_tag="JQR05_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY5325763.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVQGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2030..2105
                     /locus_tag="JQR05_003751"
     tRNA            2030..2105
                     /locus_tag="JQR05_003751"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2063..2065,aa:Asn,seq:gtt)
     gene            complement(2143..3093)
                     /gene="cbl"
                     /locus_tag="JQR05_003752"
     CDS             complement(2143..3093)
                     /gene="cbl"
                     /locus_tag="JQR05_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY5325764.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3195..4112)
                     /gene="nac"
                     /locus_tag="JQR05_003753"
     CDS             complement(3195..4112)
                     /gene="nac"
                     /locus_tag="JQR05_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY5325765.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4440..4515
                     /locus_tag="JQR05_003754"
     tRNA            4440..4515
                     /locus_tag="JQR05_003754"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4473..4475,aa:Asn,seq:gtt)
     gene            complement(4571..5506)
                     /gene="ldtA"
                     /locus_tag="JQR05_003755"
     CDS             complement(4571..5506)
                     /gene="ldtA"
                     /locus_tag="JQR05_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5325766.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLIVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5568..>5687)
                     /gene="cobT"
                     /locus_tag="JQR05_003756"
     CDS             complement(5568..>5687)
                     /gene="cobT"
                     /locus_tag="JQR05_003756"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY5325767.1"
                     /translation="GAALAMSIIEAACAIYNNMGELAASNIVLPGNTTSDLNS"
BASE COUNT         1600 a         1465 c         1234 g         1388 t
ORIGIN      
        1 acttcatgga acagaatttt tgaccttgtg aatcgtgatg tctgttgggg aggggcaatt
       61 tgctcactga agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc
      121 cactttttta caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc
      181 atagagatgt ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac
      241 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa
      301 tattagcgat gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg
      361 aaataatctg cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg
      421 ctcggatcga gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac
      481 ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag
      541 catgattccc tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac
      601 ccacatggcg taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc
      661 ggaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga cgtgtttaac
      721 ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc
      781 gatggccgtt aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg
      841 cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc
      901 acttccgggt aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac
      961 gctggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac
     1021 tgattcgaca ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag
     1081 cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac
     1141 ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac
     1201 aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
     1261 aagaatattc aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag
     1321 tgccccgcta ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag
     1381 ataagtcaac gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat
     1441 ttgttcgcca aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac
     1501 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
     1561 tacaacgaca gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa
     1621 gctgtccgcc aaccccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt
     1681 gctcaggacc cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg
     1741 ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc
     1801 gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg
     1861 aaaccacatt tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
     1921 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat
     1981 cgggtttgac aaaatatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
     2041 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     2101 agccaaattt ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat
     2161 ttcctcttca ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt
     2221 acaaagttcc agaaagcgcc aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa
     2281 ccagacagta ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc
     2341 ttgttcgccg ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt
     2401 tttaatgaca tcagaatcct gtgcacttaa tacaatatct gccggcaaac ctttgcgggc
     2461 aaatgcgtca tcagtgcgtg agcgccccgt aattccctgt cggtaagtaa ttaacggcca
     2521 cttcgctatt gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac
     2581 aagcaaacta tggtgccaac aaaaccacgg gaaggcgacg agctgcgggt cattactcaa
     2641 acgctcgctg gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg
     2701 tggcgaaccc tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat
     2761 gacctctggc aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga
     2821 cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg
     2881 ttctgcaatg accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg
     2941 tcgaacaaat atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctaacgcc
     3001 tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc
     3061 ctcgcggatt atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc
     3121 cgttattgtt aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca
     3181 tcccgatata acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta
     3241 atcactgaca ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg
     3301 ggtaaattta aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca
     3361 tttactgcgc cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg
     3421 ctggcaatcg ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg
     3481 agccgccgta gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg
     3541 agaaaaaggt tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga
     3601 gttcctacca ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg
     3661 gaatgctcat aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact
     3721 gcaccactat tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt
     3781 aataagggca tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc
     3841 cccgataatg actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa
     3901 atggcccgtg catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt
     3961 gtgcgaatca aaagctgttg atttaactca ccttccagta tggcaacctg ctggctgagc
     4021 gctggttgtg cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt
     4081 tttacgaagt atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc
     4141 aagtgccgcc attacttaca accagtatgc aagatgcctg ccagttctat tttggcgttg
     4201 atgcgcaagc taaccaactg tcaaataaga gattatgata gattcgtcat tggccctttt
     4261 tatcagcagt cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta
     4321 cccaataaga ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaaat
     4381 gcatttcacc ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt
     4441 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4501 cagtcagagg agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt
     4561 accaacgctc ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc
     4621 gatgttgcaa cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc
     4681 gcaaagatgg ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc
     4741 gcgacagcgg ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca
     4801 ctggctgatc aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga
     4861 tatcgtcatt acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg
     4921 cattggtacc gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt
     4981 tatcgggccc cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc
     5041 gggtgttcgg cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc
     5101 agttacgcgg ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat
     5161 tactgtctgg cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac
     5221 gaacagtggc gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca
     5281 aaaaaacatc agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg
     5341 cggcaaaagt ctccagcggc tgagtattgt gatcaggaac agttacaata agcgactgcc
     5401 ccactaaacg gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg
     5461 caaaaagcag agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag
     5521 tcgcgaatac tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa
     5581 tcagaagtcg tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg
     5641 tatatcgcac aagcagcttc gatgatggac atcgccagag ctgcacc
//
LOCUS       DADKXM010000192         5675 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.195, whole genome shotgun sequence.
ACCESSION   DADKXM010000192 DADKXM010000000
VERSION     DADKXM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.195"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..785
                     /gene="msrP"
                     /locus_tag="JQR05_003757"
     CDS             <1..785
                     /gene="msrP"
                     /locus_tag="JQR05_003757"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY5325768.1"
                     /translation="NLPLTPADKVSGYNNFYEFGLDKDDPAANAGSLKTDPWTLKISG
                     EVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAEPTSNAKY
                     VAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNG
                     APVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQ
                     ATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
     gene            786..1421
                     /gene="msrQ"
                     /locus_tag="JQR05_003758"
     CDS             786..1421
                     /gene="msrQ"
                     /locus_tag="JQR05_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY5325769.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1679..2329
                     /gene="zinT"
                     /locus_tag="JQR05_003759"
     CDS             1679..2329
                     /gene="zinT"
                     /locus_tag="JQR05_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY5325770.1"
                     /translation="MAIRLHKLAVALGVFIVSAPTFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2999..3088)
                     /locus_tag="JQR05_003760"
     tRNA            complement(2999..3088)
                     /locus_tag="JQR05_003760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Ser,seq:cga)
     gene            3182..3979
                     /gene="mtfA"
                     /locus_tag="JQR05_003761"
     CDS             3182..3979
                     /gene="mtfA"
                     /locus_tag="JQR05_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY5325771.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEIAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4080..4155
                     /locus_tag="JQR05_003762"
     tRNA            4080..4155
                     /locus_tag="JQR05_003762"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4113..4115,aa:Asn,seq:gtt)
     gene            4317..5579
                     /locus_tag="JQR05_003763"
     CDS             4317..5579
                     /locus_tag="JQR05_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5325772.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1505 a         1328 c         1310 g         1532 t
ORIGIN      
        1 ataacctgcc actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg
       61 ggctggataa agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac
      121 tgaaaatcag cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc
      181 gcttcccgct ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg
      241 tgccgtggat tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg
      301 cgaagtatgt cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc
      361 gctttatcgg cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa
      421 tgcatccgct cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg
      481 gcgcgccggt gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg
      541 tcagtattaa gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg
      601 aatacggttt ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta
      661 ccgaacgatt tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt
      721 ttaatggtta cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt
      781 tctaaatgcg tctgacagca aaacaggtga tatggctgaa agtttgcctg catcttgccg
      841 gattgttgcc gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc
      901 cggtgaaaga tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct
      961 tgttaatcac acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc
     1021 tgttaggctt atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg
     1081 agttgggcgt gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa
     1141 cgttaggaat tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga
     1201 tgcagcgaaa actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga
     1261 tcctggcccc aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct
     1321 acgctgggct ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca
     1381 accggttacg caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac
     1441 ttcgcgttat cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc
     1501 aagttatgtt tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata
     1561 agatagttca atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt
     1621 ttcatatgtt acaatataac attacacata atgtacatta actctggagg aaattgtttt
     1681 ggcgattcgt cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctac
     1741 cttttcgcat ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc
     1801 taatggtgtt tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt
     1861 ctggcaatcc gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa
     1921 agcggatgca gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta
     1981 tccgacagat atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa
     2041 tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg
     2101 caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata
     2161 tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat
     2221 gggtaatgat tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc
     2281 ctatcagttg agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc
     2341 tgacttcagt tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa
     2401 ataagtaaga caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt
     2461 agaaaaaaac tgagaaaaag gcgaacaata aatacacaac tcagccatac agactggcag
     2521 cgcaaatgtt tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta
     2581 attttggtgt ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg
     2641 ggaaattcaa caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg
     2701 cgttcctttt ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca
     2761 actttgcggc agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac
     2821 gactgaataa ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc
     2881 gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta
     2941 cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaagtg
     3001 gcggagagag ggggatttga acccccggta gagttgcccc tactccggtt ttcgagactg
     3061 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
     3121 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
     3181 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
     3241 ggaagcacta tcgatccccc ttttaacgtg tctgacagaa caggaacaaa gcaaattggt
     3301 cgctcttgcc gaacgttttt tacagcaaaa acggcttgtt cctttacagg gctttgagct
     3361 gaattcatta agaagctgcc ggatagcact tctattttgc ctgcccgttc tggagttagg
     3421 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
     3481 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
     3541 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
     3601 ttctggtttt aacctgatta ttcatgaaat cgctcataag ctggacaccc gtaacggcga
     3661 tcgcgccagc ggagttccct ttatttcgtt gcgtgaggtt gctggctggg aacacgatct
     3721 tcatgctgca atgaacaaca ttcaggaaga aatcgaatta gttggtgaga atgcggcgag
     3781 cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     3841 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgtca
     3901 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     3961 ggcgacgaat gttcattaat taacaacttt gcagattaat taaccaattg aaatggctta
     4021 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tctcacgatt
     4081 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4141 cagtcagagg agccaatttt ctgttttcat gcatccttgc gaatctttat ctgatgttga
     4201 ttcaacaggt tagtgtgaaa actcttctcg gtgcattttg attttaccct acgcatcggg
     4261 aaaaattggt ggtcaaatct ggggtcaggt tagttcgata atggagtgac ccccatatat
     4321 cccttaccga cgcaaaaatc cgcaccctca agccttctga taaacccttt aaagtctccg
     4381 attctcacgg tctgtatctg ctggtcaagc cgggtggctc ccgccactgg tatctcaaat
     4441 accgtattag cggtaaagaa tcccgcattg cgctgggtgc ctatccagcc atctccctgt
     4501 ctgatgcgcg acagcaacgt gaaggtatcc gtaaaatgct ggcgctgaat atcaacccgg
     4561 tacagcagcg ggctgctgaa cgtggctcac gaacaccgga gaaagttttt aaaaacgtgg
     4621 cgctggcgtg gcataaaagt aacaggaaat ggtcgcagaa caccgccgac cgtctgcttg
     4681 ccagcctgaa caatcacatc tttccggtca tcgggaacct acctgtatca gaacttaaac
     4741 cccgtcattt cattgacctg ctgaaaggga tcgaggaaaa aggtctgctg gaggttgcgt
     4801 cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag gagttaatcg
     4861 atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga cggcactatc
     4921 ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg tattggggca tatcatcagg
     4981 gccgtgaact gacccggcat gccgttctgc tgatgctgca tgtgttcatt cgctccagtg
     5041 aactgcgttt cgcccgctgg tcagagattg atttcacaaa ccgagtctgg acgatacccg
     5101 cgacgcgaga acccattatt ggcgtgcgtt attccggccg cggggcaaaa atgcgaatgc
     5161 cgcatatcgt ccccctctca gaacagtcca tcgccattct gaaacagatt aaggatatca
     5221 ccggtaataa tgaactgatc ttccccggcg accataaccc gtataagcca atgtgtgaaa
     5281 acacggtcaa taaggcactg cgggtgatgg gttacgacac gaaaaaggat atctgcggtc
     5341 acggcttccg ggcaatggca tgcagtgcgc tgatggaatc gggtttatgg gcaaaggacg
     5401 cagtagaacg ccagatgagt catcaggagc gcaataccgt gcgcatggct tatattcata
     5461 aggcagagca cctggaagcc cgcaaagcga tgatgcagtg gtggtcggat tatctggaag
     5521 catgccgaga atcttatgca ccgccttata caattggtaa aaataagttt atcccatagt
     5581 atactaacga atcccacact ctggcaaatt accaccacct ctacaggagg tggttctata
     5641 tagactttta tcaacttcat gacgaaaaac accta
//
LOCUS       DADKXM010000193         5670 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.196, whole genome shotgun sequence.
ACCESSION   DADKXM010000193 DADKXM010000000
VERSION     DADKXM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.196"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..977
                     /locus_tag="JQR05_003764"
     CDS             <1..977
                     /locus_tag="JQR05_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HAY5325773.1"
                     /translation="GFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLV
                     APLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
                     SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFI
                     GAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLF
                     LTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAALGFISAIGAVG
                     GFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1059..4799
                     /locus_tag="JQR05_003765"
     CDS             1059..4799
                     /locus_tag="JQR05_003765"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY5325774.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENAHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EEIGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGCDQVQEAKK"
     gene            4796..>5670
                     /gene="narH"
                     /locus_tag="JQR05_003766"
     CDS             4796..>5670
                     /gene="narH"
                     /locus_tag="JQR05_003766"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY5325775.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         1328 a         1528 c         1550 g         1264 t
ORIGIN      
        1 gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa
       61 aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg
      121 taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt ctcggcgtca
      181 atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg
      241 taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt
      301 cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga
      361 gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc
      421 tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta
      481 ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg
      541 tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac
      601 cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga
      661 ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa
      721 ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga gaagccgtca
      781 ccgaaacggc ggcagcgctg ggctttatct cagccattgg cgcagtgggc ggctttttta
      841 ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag
      901 tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga
      961 agttcagcca aaaataagag atcgatgcct gatgtacaac cgtgtcgcat ccgacaaggc
     1021 atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct
     1081 ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata
     1141 gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt
     1201 ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc
     1261 tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc
     1321 atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc
     1381 gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gcactcaaac
     1441 aacacagcga tccggtactg gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga
     1501 gctacaaaca agtgcgtggg cgcggcgggt tcatccgctc caactggcag gaactaaacc
     1561 agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg
     1621 gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt
     1681 cgctgcttgg cggcacctgt ttaagtttct acgactggta ttgcgacctg ccgcccgcct
     1741 cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca
     1801 gctacatcat cgcctggggg tctaacgtac cgcagacacg tacgccggac gcccacttct
     1861 ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag
     1921 tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga
     1981 tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta
     2041 tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag cctcgcgacg
     2101 atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg
     2161 aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc
     2221 cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg
     2281 ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag
     2341 gcgtggcctt cccctacttt ggcggcattg aaaatgcgca ttttcgcagc gtaaaacaca
     2401 atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct
     2461 gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg
     2521 aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg
     2581 agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata
     2641 ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt aaccactggt
     2701 atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg
     2761 ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg
     2821 gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag atgaacagca
     2881 cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt
     2941 tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc
     3001 gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga
     3061 ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga
     3121 aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta
     3181 atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac gagtatatgc
     3241 agaagtatct gctggggacc gaaagcggga ttcagggcga ggaaatcggt gccagcgacg
     3301 ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc
     3361 tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca
     3421 ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca tttattcatc
     3481 cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa atctacaaag
     3541 gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa accgacgtgg
     3601 tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt gaagtgctcg
     3661 actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat attgtggcgg
     3721 tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca
     3781 aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg
     3841 gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg
     3901 ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag
     3961 tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc
     4021 acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct
     4081 ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca
     4141 acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc
     4201 atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc
     4261 gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa gcacttaact
     4321 tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc
     4381 tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga
     4441 ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg actgcccgcg
     4501 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
     4561 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
     4621 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
     4681 ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc cgcaagatga
     4741 agaacgttaa ctggctggat gatgaaggtt gcgatcaggt acaggaggcg aaaaaatgaa
     4801 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
     4861 ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
     4921 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     4981 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
     5041 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
     5101 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
     5161 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
     5221 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
     5281 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
     5341 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
     5401 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
     5461 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     5521 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     5581 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     5641 agcggcgagc accgagcgcg aagttgacct
//
LOCUS       DADKXM010000194         5668 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.197, whole genome shotgun sequence.
ACCESSION   DADKXM010000194 DADKXM010000000
VERSION     DADKXM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.197"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..730)
                     /gene="araH"
                     /locus_tag="JQR05_003767"
     CDS             complement(<1..730)
                     /gene="araH"
                     /locus_tag="JQR05_003767"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY5325776.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLSLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASR"
     gene            complement(742..2256)
                     /locus_tag="JQR05_003768"
     CDS             complement(742..2256)
                     /locus_tag="JQR05_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY5325777.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRNNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2326..3315)
                     /locus_tag="JQR05_003769"
     CDS             complement(2326..3315)
                     /locus_tag="JQR05_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5325778.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4112..4615
                     /locus_tag="JQR05_003770"
     CDS             4112..4615
                     /locus_tag="JQR05_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY5325779.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4695..4946)
                     /locus_tag="JQR05_003771"
     CDS             complement(4695..4946)
                     /locus_tag="JQR05_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY5325780.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5061..5147)
                     /gene="azuC"
                     /locus_tag="JQR05_003772"
     CDS             complement(5061..5147)
                     /gene="azuC"
                     /locus_tag="JQR05_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY5325781.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5410..>5668
                     /locus_tag="JQR05_003773"
     CDS             5410..>5668
                     /locus_tag="JQR05_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325782.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSL"
BASE COUNT         1487 a         1447 c         1273 g         1461 t
ORIGIN      
        1 tacgtgaagc cagaataatt ccggctatcg cagataccag gccagagaga acaaagataa
       61 taattttggt gcgaacaacc ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa
      121 tcgccagggt attacgacca aaggtggttt tattcagcag caaaccaaag ataatcagac
      181 acgcgacggt gagccagatt ggcgcaggca gaccgaacca gttagcgtaa ccaagggcaa
      241 agaagctttc atcttcgata ccgaccgctt taccgtctga aatgatgtac gccagacctc
      301 gaacaatctg catcgttgcc agtgtcgtga tcagagcatt tattttcagt ttggcgataa
      361 caaagccatt gaccaggcca cagagaacgc ccagcgacaa acccgctgcc acgccaatcc
      421 acaggctttc agtcaggttg ataaccaccg ccgtggtgac acccgcacag gcaattacgg
      481 aggcgacaga aaggtcaaag tcaccggaag caaggcagaa caacatgcca cacgccacca
      541 tccccgacat ggaaattgcc aggcccaacc ctttcatatt aatgaaggtg gcaaaatttg
      601 ggacaaaaat ggcacaggcg ataaagagca ccgcaaacac caccagcatg ccgtactgat
      661 cccagatacg cccgaagctg aatgacgact taggtgcgcc agaccccgat gtagaaacag
      721 aagacatcat actctcctta ctcaggcaac tgcctggctg actttaggca tcgcaaggct
      781 cagtgcctga cgctcatctg cctgctcgtg taacaattca ccggcgattt cgccttcccg
      841 catcaccaca atccggtcgg caacgccgag gacttcaggt aagtcgctgg aggcaaacag
      901 caccgccacg ccctgcgccg ccagcgcata aattacgtta tatatttcgt gcttagcgcc
      961 aacatcaatg ccgcgcgtag gttcatccag caaaatgacc ttcatctctt ccgataacca
     1021 gcggcccaga atggcttttt gctgatttcc gcctgagaga ttcatgatca gttgctccgc
     1081 gcccggcgtt ttgatgttga gcgaacgaat gtgctgatcg gcattgtttt cttcccaact
     1141 gttgttgatt acacaaccgc cgagcacatg tttgcgtctg gcactgatgt tgatattgtt
     1201 gcgaacggag tgcacgggaa taatgccttc cgctttgcga gcttccgggc agagcatcat
     1261 gcctgcggca atggcgtggc tcggtttacg aatatcgatc ggctgttggt cgatataaac
     1321 ctgaccggcg gtgatttgcg tcccgccaaa caagcctttc attaattcgc tacgccccgc
     1381 ccctaccaga ccaaacagcc caacaatttc accactgcga accgccagac ttattggcgt
     1441 acgcacgcct ggtgctttca cagcatcaag acgcaggcgt tcctcgccat aactacgagg
     1501 ttgccagccg tagatatcgc caatgtcgcg cccgaccatc gcctgcacca gcgcgtcgtg
     1561 gtcaacctgc tgcatatcgg taaaggtttt gacataacgt ccatctttaa agacagtgat
     1621 ggcatcgctg agggcaaata tttcttccat acggtgagaa acgtataaga tcacccgccc
     1681 ctcttttcgc agttcacgaa taacgcggaa aagattgccg atttcgcggg cagaaaggga
     1741 gctggttggc tcatcaaagg cgataatttt agcgttacgc gccagcgctt tggcgatttc
     1801 aaccatctgc cactgaccaa tggagagata tttcagcggc gtgtccgggt caatatccat
     1861 accaagatgt ttaagttgta aacccgcctc ataattcagc aatgagcgat tcacaatgcc
     1921 gcctttatgc ggcagctggc cgagatagat gttttccgcg acggtcattt ccggcacgag
     1981 atgcagttcc tggtaaataa tcgccacacc cgcgttaagt gctgcggtcg tgtcggaaaa
     2041 ggacatttcc tgcccattaa tcactacaga acccgtggtt ggcgcatagt tgccgctgag
     2101 gatttttaag agagttgatt ttcctgcgcc attttcaccc atcaacgcat gaacctgacc
     2161 ggcatagcag tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg ttttaccgat
     2221 gccgcgaaat gagagatacg gggtagactg ttgcataacg tctccgtgaa tcactggtcg
     2281 ttcacacccc ctctgcatca tgcagagggg aatttttccg gcaaattact taccgcctaa
     2341 accttttttc tccagttctt ctttaaagtt gtcacgcgtg atcagtacca cgtctgtaac
     2401 ttcggtaaat tttggcgttt caacgtcttt tgctacccag ttgtaaagca tttcgctgga
     2461 tttatagcca tgtacgtccg ggcttggcag cagggaaccg tagaagccgg ttgcctgtgc
     2521 tttagacagt tcgcttactg catccacacc gttaatacca ataccgatga tatcggctgc
     2581 tttaaagccc tgaccttcgg ttgcacgtac gccgccaagt acggtgctgt cgttcatacc
     2641 gacgatcagc caatgtttaa cttccggatg ttgaaccagc attgagttgg cagcgtcaaa
     2701 tgcccccggg atgtcgttag atttggtagg tacctgataa atttgttttt ccgggaatcc
     2761 tgcagctttc agtgcgtcca tagaacccgt tgtacggcgg cgggcggtat ccagttcgtt
     2821 ggcggtaatc gccatcactg cgctttcttt gacatcccag ccacgtttct gcatctcttt
     2881 atacagttcc tggccctgac gttcgccaat tttagtcgcc gccatcatca ccagaggaac
     2941 ggtatccatt ggcttacctt tggcgttaac aaactggtca tccactgcaa tgactttcat
     3001 atcgtagcca cgcgctttcg cgacgatggc agagccgagt ttcgggtccg gagtacaaat
     3061 aacgaaacct tttgcgccac tggctgccag gctgtcgatc gcgttcaatg ttttttcgcc
     3121 atccggcacg gcaatcttaa taacctcaaa ccctaaatcc ttcccggctt tatcggcaaa
     3181 cttccattcg gtctggaacc acggctcttc cggttgcttc accagaaaac cgagcttcag
     3241 gttctccgcc atagcggatt gtgacataac ggctgccaga ccaatggctg ccagggcttt
     3301 agtaaatttg tgcatggttc tctccagctt tagtgtcgtt ttgtgtaggg caaaaacgaa
     3361 tgacattcgt taaattaatc ggaaaacaaa gcattacctt ttatctaaaa gataagtgac
     3421 cgtgttgaca tagttttagc gagaaattaa ttctccatag gagagcaata tcacatcgca
     3481 gaattacagt gagaacgtgc ataaatttag cgggaaaaga cataaggaaa agccaatttg
     3541 tcagacaaat tgttgaatgc acagcagatt aatccataag attagcctgg aaatccttgt
     3601 tgtctttggt acccatgcgg gatgtcttct ttttaaccag tcaataggcc gcattacctg
     3661 gcgttgagtt tttgaaatgg tgtaataacc acaactcaaa gatgtggaaa atgcgcgtca
     3721 ttcatttcgc cattaattat cactgtgctc attaattaac agaacacgta taatgagagc
     3781 catctcgcaa aaatgaaaaa acgttttata aaatcatcac ttcatcatga attcaaattc
     3841 atcgattaat atcaacaaga tacaaaaagc actatcatta aaattcattg catttacatt
     3901 gatttcacca atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttataac
     3961 cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg
     4021 gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac
     4081 ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact
     4141 caactctcaa atgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg
     4201 gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa
     4261 tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt
     4321 taaagccatt gatgttccag gtgaaaaact gaactctctg gaagaactgt tccaaaaaac
     4381 gatggaagaa tacgagcaac gttcaagcac gctggcgcag ttagccgatg aagcgaaaga
     4441 actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga
     4501 tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg
     4561 ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca caccagctgc attgatcata
     4621 catcggcgct aatacattgc gccgatgaag tttttgagaa accgctgcct catctgtttg
     4681 aagcagcggt ttttttaatg ggattcaccc tgtggggtaa acttgagttc aatcagcgcg
     4741 atggcttttt ggattgcccg catggtgacc gggtctgcgg cagcgggatg atgagtaaag
     4801 tcgatattct tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg
     4861 agaacatcca gaatttgttt gataaccaac tggcaagcta ccacatcaga aaccagttcc
     4921 tgatcggcat tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat
     4981 agtagcggct tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac
     5041 ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc
     5101 ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga
     5161 ccatttgtta tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat
     5221 caatctttat gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca
     5281 aaaaacagcc cccctatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt
     5341 cttttccggc cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct
     5401 ggctggacca tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg
     5461 gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt
     5521 tatgaccagg cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc
     5581 gatcgcgcat gccagcagga aggctatacc cacgcgatcc cctttggtca gccagtaggc
     5641 aactgtagcc tttttgcggg ttccttat
//
LOCUS       DADKXM010000195         5518 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.198, whole genome shotgun sequence.
ACCESSION   DADKXM010000195 DADKXM010000000
VERSION     DADKXM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.198"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQR05_003774"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQR05_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY5325783.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JQR05_003775"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JQR05_003775"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY5325784.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHQTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JQR05_003776"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JQR05_003776"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY5325785.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JQR05_003777"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JQR05_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY5325786.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1359 a         1328 c         1500 g         1331 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac cagaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgttc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaagtg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctga
//
LOCUS       DADKXM010000196         5354 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.199, whole genome shotgun sequence.
ACCESSION   DADKXM010000196 DADKXM010000000
VERSION     DADKXM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.199"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(304..747)
                     /locus_tag="JQR05_003778"
     CDS             complement(304..747)
                     /locus_tag="JQR05_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325787.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            1262..2104
                     /locus_tag="JQR05_003779"
     CDS             1262..2104
                     /locus_tag="JQR05_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325788.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            2211..2474
                     /locus_tag="JQR05_003780"
                     /pseudo
     CDS             2211..2474
                     /locus_tag="JQR05_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            3101..3646
                     /gene="cobU"
                     /locus_tag="JQR05_003781"
     CDS             3101..3646
                     /gene="cobU"
                     /locus_tag="JQR05_003781"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY5325789.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDDGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3643..4386
                     /gene="cobS"
                     /locus_tag="JQR05_003782"
     CDS             3643..4386
                     /gene="cobS"
                     /locus_tag="JQR05_003782"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY5325790.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            4398..>5354
                     /gene="cobT"
                     /locus_tag="JQR05_003783"
     CDS             4398..>5354
                     /gene="cobT"
                     /locus_tag="JQR05_003783"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY5325791.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQVGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEG"
BASE COUNT         1414 a         1117 c         1387 g         1436 t
ORIGIN      
        1 ccggctcatc aaccatttgc cggagcagat tgatgctcag ttgcaaaagc cggtcggctt
       61 catcacaaag cagggtgatg ttcttttcgt gcattattta accttcctga gacgcatttt
      121 catgacaacc acggaggagg ccatttacta caaacgatcg tttatagcga tcaatataat
      181 agcaacgatc gttatattct attaatataa aaaacacaac gcaaggggga caacccgggt
      241 atggctgcgt ttgcattgtg ttttcataga gttaaaaatc tgtaacgaca acccggcaca
      301 tcactaccgg gttaagaaga aaatccagtt ccataccgcg cgggccacag agaccacggt
      361 gtcacgaacg gcgcgcaggg ccgtacggac aacagaggca acacagacac tctccgccgt
      421 gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gtctgagtgc ggacagactc
      481 cgtgcgcagt tcgtcctgca ggcgtgtcat cagagggatg gcggcatgtc cgggaagcac
      541 tgtcatgagt gcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga
      601 tttaccggcc ccgcttttac ccataatgcc aggcacgggt tcgtgacggg ggagtttgcg
      661 cagatgttcc aggatgtgac gggaaatcgg gtaaggcagg gaggagagcg gtttttcaat
      721 tgcctcaatg gcatcagacg gattcataca tgtattccca gtgaaaaaag acaaaacccc
      781 gtgcaccgga aaggtggcgg gggattcacg gagataatat ctttctgaaa ttattttgaa
      841 tgcaggtata tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac
      901 agtccgcttt gtgccaggag cggactgacc tgctccccga tgattaatac acagctatgc
      961 tagtaatgtc tgtagatcgc tgtgtgttgc atctatcggt tgaactcaca gtccaaacca
     1021 gactgtcagc tttgattaga ttctacctac tcaacctgtc agataaggtc tgagctaata
     1081 catctaatat atggctttgt gcaggggacg gctatcgcga acgttaggct aggtatctga
     1141 atacgcacag ttcacttggt tgtacttctt gcactgatct gctatcccgc acagctattt
     1201 ataggtaatc ttcagtgtat aatctgccga cttatggaag cttgtacaac aggagaaata
     1261 tatgaaaaaa gaattgtttg gacgttgccc gtttgatctc gacgaagatg taaaaaaaat
     1321 agccgcgata aataaatata tccatgcaaa atttgaggag cataaaaata aaatagacaa
     1381 cctaaagggt gtggaacaaa aattgatggt cttacagtac tttaatgtac tgaatgaact
     1441 tagtaatcag gctatctctt ctttgaatat aggagccttt tcagcatcgg aagtattagc
     1501 tagggttata atagaacagg ctgccaatca attttatata gcaatagatg atggcgaaaa
     1561 tgcacaagca ttactcaagg ctagtaagaa attagttcat agtaatggaa aacggtggct
     1621 ggagtgttta aaatccaaag gaatgactaa tccagcagca aatgaaagga ttcgcatggg
     1681 aaaagaacta actgatctat ttaatagatt gtggccaaat acacctgaat atccaggaac
     1741 cagaaaactt tttgaattaa ttggctggga aacacactat catgcccatt acgttcccct
     1801 ttgtgattca atacatacat tctctgatga tatggtaaat atagtatctc tatataatgc
     1861 aatccaaagt gatgaaacta ctgctatcga attcattctt gcagttaaac aagaaaataa
     1921 acggctggca atttataatt ttgtaattgc tacggccctt cggtgtgagg ccttagtaaa
     1981 tgtatttaac tctttaggtt atcaggatat tataacagaa atgacaccaa caatagatac
     2041 agtcaaccag actataattc gatatgatga ttttgatcat tccagaattg atgattgtca
     2101 ataattaatt gacagaatac gtacatgcgc aacaactagt gatagttgac ttattttcgc
     2161 tcttgagaga caaccatact caaatctccc acattgcagg agatttgaat atgaaaacgt
     2221 caccgtggaa taaagaccgt accatcggtc aaaaaaggcc acttcagata tctcatatct
     2281 gggggatccg gattagactt gagttggaag gtaaaacacg cgatctagct ctgttcaaca
     2341 tggctttgga cagtaagcta cggggctgtg atttggtcaa actcaaagta tcagatgctg
     2401 catatggcag ttctgtctca agcagagcaa cggtgttgca acagaaaacc ggcagccctg
     2461 tccaatttga gatgccttta ataacacatc ttttaaatgt tgaagaatta tcccgcctgc
     2521 tgaagaacgt tgctctgagt gtgataaacc tttttattct tgatgatgtc agcgagaaga
     2581 tggaatatat ccccgactgg gtcgtggtta cccggttaaa catctggtgg cgctaataaa
     2641 tctggcaagt cacattctgt tgaatgctgt gacagtgcct taccgagaga gcgaaattgt
     2701 ggtgttctag tgcatagtcg ccaccattcc ggataattct attgcgttgt tttacaagtt
     2761 cttttacagc gcagatctgt cgctgacgtt gaatcaatag cctggatttc agaacacaat
     2821 gcccgtttgc tggttggtcc ggcagtagca ttaattcatt atctgaatga aatgtgtcaa
     2881 tccacagaaa ccgctgtcaa tgacgcattt tgtcggacgt taacacgaga aagtctggct
     2941 attccgtaat gccatcttat ttcgtaatgg attgagaatg ctggattttt aattatatta
     3001 cgcgcagtat taatcgcgta aatgttttgc ggtcagcaac ggtgggggat ccattgctct
     3061 gttcaatgaa taccatcata catttgaatc aggagtcggt atgatgattt tggtgacggg
     3121 aggcgcacgg agcgggaaga gtcgccacgc agaggtgctt attggggact cttcacaggt
     3181 tctgtatatc gctacctcgc aaatccttga tgatgagatg gctgcacgga tagaacatca
     3241 tcggcaaagc cgcccggagc actggcgcac agtggagcgc tggcaacatc ttgatgaatt
     3301 aattcatgca gacattaacc cgaatgaggc tgtgttgctg gaatgcgtta ccacaatggt
     3361 gactaatctg ttgtttgatg atggcggcga taaagaccct gatgaatggg attatcaggc
     3421 gatggaacag gcgattaatg ctgagattca gtcgttgatt gctgcctgcc aacgttgccc
     3481 cgcaaaggtt gtattagtga ctaacgaagt gggaatgggg attgtgccgg agagtcgtct
     3541 ggcacgacat tttcgtgata ttgccgggcg ggtaaatcag cagttggccg ctgcggcaaa
     3601 tgaagtatgg ctggtggttt cgggtattgg agtaaaaatc aaatgagcaa attattttgg
     3661 gcgatgctct catttattac gcgtctgcct gtaccgcgtc gctggtccca gggactggat
     3721 ttcgagcatt attctcgcgg tattattact tttcctttga ttggattatt gcttggcgcg
     3781 attagcgggc tggtcttcat ggtgctgcag gcatggtgtg gcgcaccact ggcggcactg
     3841 tttagcgtac ttgtgctggc gctgatgacc ggggaattcc atctggacgg cctcgctgat
     3901 acctgtgatg gcgtattttc tgcacgtagc cgcgatcgca tgttggaaat catgcgtgat
     3961 agccgtttag gcacccacgg cggtctggca ttgatttttg tggtactggc aaagattctg
     4021 gtgttaagcg agttggctct gcgtggcgaa ccgatccttg catcgctggc ggcagcatgt
     4081 gcggtcagcc gcgggactgc cgcattattg atgtatcgtc atcgttatgc gcgggaagaa
     4141 gggcttggca atgtgttcat tggcaaaatt gatgggcgac aaacctgcgt cacactcggc
     4201 ttagccgcta tttttgctgc cgtattattg ccgggtatgc atggtgtggc tgctatggtg
     4261 gtgacgatgg tggcgatttt catcctcggt cagcttctca aacgtacgct gggcgggcaa
     4321 acgggcgata cgctgggagc agccatagag cttggtgaac tggtcttcct gctggcactg
     4381 ctataaagag acgtgacatg caaataattg ccgatttact gaatacgatc ccagccatca
     4441 attctgccgc tatgtcgcgt gcacaacggc atgttgacgg gttactcaaa cctgttggta
     4501 gcctaggaaa gctggaggcg cttgccatac aactggcagg aatgccgggg gtgaatggca
     4561 taccgcatgt gggcaaaaaa gcggtactgg ttatgtgtgc cgatcacggc gtctgggagg
     4621 aaggggtcgc tatttctcca aaagaagtga cagccataca ggctgaaaat atgacccgtg
     4681 gaacaaccgg cgtgtgtgtg ctggcagcac aagtgggcgc taacgtccac gtaattgatg
     4741 ttggtattga tactgctgag cctatccccg ggcttatcaa catgcgtgtc gcacgaggta
     4801 gcggcaatat tgcttcagct ccggcaatga gtcgccgtca ggctgaaaag ttgcttttgg
     4861 acgtcatatg ttatacgcgg gagctggcaa aaaacggtgt cacgctgttt ggtgtaggtg
     4921 aactggggat ggcaaacacg acaccggcag cggcaatagt cagcacaatc actggccggg
     4981 atcctgaaga agtggttggg attggcgcaa acctgccgac agataaactg gctaataaaa
     5041 ttgatgttgt gcgtcgggcg attacgttga atcaaccaaa tcctcaggat ggtgttgatg
     5101 tcctggcaaa agtgggtgga tttgatttgg tcggaatagc tggagtgatg ttaggtgctg
     5161 cttcctgcgg tttacccgtg ttgctggatg gatttctttc ttatgctgct gcgctcgcag
     5221 cctgccagat gtctcctgca atcaaaccgt atctcattcc ttctcacttg tcggcagaaa
     5281 aaggcgcgcg tatagcgctc tcgcatttgg ggctggagcc ttatctcaat atggatatgc
     5341 gtttaggtga gggg
//
LOCUS       DADKXM010000197         5320 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.200, whole genome shotgun sequence.
ACCESSION   DADKXM010000197 DADKXM010000000
VERSION     DADKXM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.200"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1374)
                     /gene="caiC"
                     /locus_tag="JQR05_003784"
     CDS             complement(<1..1374)
                     /gene="caiC"
                     /locus_tag="JQR05_003784"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY5325792.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDHPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSI"
     gene            complement(1448..2665)
                     /gene="caiB"
                     /locus_tag="JQR05_003785"
     CDS             complement(1448..2665)
                     /gene="caiB"
                     /locus_tag="JQR05_003785"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY5325793.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRSITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            complement(2794..3936)
                     /gene="caiA"
                     /locus_tag="JQR05_003786"
     CDS             complement(2794..3936)
                     /gene="caiA"
                     /locus_tag="JQR05_003786"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY5325794.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(3967..>5320)
                     /gene="caiT"
                     /locus_tag="JQR05_003787"
     CDS             complement(3967..>5320)
                     /gene="caiT"
                     /locus_tag="JQR05_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY5325795.1"
                     /translation="FEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFAS
                     CTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVA
                     FAYFFFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPL
                     VTECMQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGW
                     VFIVSGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQ
                     MSIFLARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQY
                     GVARAIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPL
                     LVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD
                     "
BASE COUNT         1267 a         1443 c         1371 g         1239 t
ORIGIN      
        1 aatcgaatct ttaataccca caaccacgat gtcctgaatt ttcggatggg tggcgatgat
       61 attttccagc tccacgcagg agacattctc gccaccgcgt ttgatcatat tgcagcggcg
      121 atcgacgaaa taaaaaaagc cttcttcgtc gcggtatccg gtatcgccgg tatgtagcca
      181 gccgtcggct tccagcactt tcgcagtggc tttcgggttg agaaaatact ctttgaagat
      241 ggttttccct ggtacgcctt taatacagat ttcaccgatc tcacctgccg ggagcgggcg
      301 attgtgatcg tcgcggatct ccgcttcgta gcaaaacccc gcccgaccaa tcgacggcca
      361 gcgtcgttta tcgctagggt gatcgccgat aatgcccaca atggtttccg tcatcccata
      421 agacgtcagc aagcgaacgc cgaagcgttc acaaaatgcg tctttttcct gctccgacaa
      481 gttgagataa aacatcactt cccgcaggcg gtgttgccga tcgttcgctg aaggcggctg
      541 caccatcaac gtacggatca tcatcggaat acattcggta atggtggcgc ggtacttctg
      601 cgcctgtccc cagaaggcgc gggcgctgta tttctcgacc agcacaaagg tggccccggc
      661 agaaaacgcc gccatcgccg cagtacactg gcaatcgata tgaaacgcag gcattaccgt
      721 catgtagacg tcatcgtcac gcagtgcaca ctgccaggcg gagtaatatc cagcgaagcg
      781 caggttgtaa tgggtaatca ccacaccttt cggtcgggag gtggtgccgg aggtgaagag
      841 aatttccgcc gtatcgtcag tcgatagcgg cggtgcatag cacaaggtgg caggttgttg
      901 atttttcagt tgagtaaacg aactcactcc atcatcagcg ggaagtgcca catctgtcag
      961 gcaaatgtgc cgcaattgag tggcatcttc ctgctgaatc tgttgataca taggataaaa
     1021 ttgcgcactg gtcaccagca ggcacgcctg gctattttgc aagatccact cgctttcctc
     1081 gcgtaacagg cgggcgttaa tcggcaccat aatcgcgcca atttttgcca gcccgaacca
     1141 gcaaaagata aattccgggc agttgtcgag atgtagtgca accttgttgc ctttgcgaat
     1201 ccccagcgta taaaacaggt ttgccgtgcg gttaatctcc tgatttaact caagataact
     1261 ataccggtta acgactccgc cgctggattc acaaatcagc gccgttttat gaccgtaaac
     1321 gtccgccaga tcgtcccaca tttgacgtag atgttgtccg ccaatgatat ccattgcacc
     1381 tctatccatt tttgttcgtt tgttattggg cgggcgctag tcaggcaagc cgactgacgc
     1441 cacgcgttta gtcctcaact ttggccagac ctttgctgac caactcctga atgtcgtttt
     1501 cgctgtagcc gatatttttc aaaatggcag ccgtgtccat gccatgtgag ggcattccgc
     1561 gccagatttg tccggggtta tttttgaatt tcggcatgat gttcggccct ttgcaggtgc
     1621 gaccatccat cgtttgccac tgagtgattg attcacgggc aacatactgt ggattgcttt
     1681 ccagttccgg tacggtcagt actttggcac aggcgatatt cagttcggca aagcgttctt
     1741 taacttctgc gatggtatgt gccgccagcc aggcatcgag tttttcttct accagtgggc
     1801 cgtaagggca ttcgatacgg tggataagct gagtgccttc cgggatttcc ggcgtaccga
     1861 gtagatgtgc gaggccaata tctttaaagc actcttcaat ttgggtaatg cccaccagtt
     1921 ccatcacgat gtagccgtcg gcacatttat acagaccgca accggcgtag tagggatctt
     1981 tacctttgct catgcgcggg cacatttcgc cgccgttgaa gtaatccatc atgaagtact
     2041 ggcccatacg cagcatcact tcatacatgg cgatgtcgat actttcgcct ttaccggttt
     2101 cacgcacttt atgcagtgct gccagcgccg ccgtggtggc ggtcaggcca gaaaagtaat
     2161 cggcggtata cgggaaggca ggcattggct ggtcaacatc accgttctga atcaggtaac
     2221 cactgaaggc ctgggcgatg gtgttatagg ccggaagatt ggtgtactcc tcggtgccgt
     2281 actgaccaaa accggacagg tgagcgataa ccagtttcgg gttgtgctgc cacagtactt
     2341 catcggtaat gctacgacgg gcaaaggccg gacctttact ggcttcgatg aagatatcgg
     2401 tggtttccat taatttcaga aacgcttcgc ggccttcatc tttgaaaata tttaacgaca
     2461 gcgcgtgcaa attgcggcgg gagagctgcg ggtagttcgg ttgaacgcga atggtgtcgg
     2521 cccaggcgac gttctcgatc caaataactt ccgcgcccca ttctgcgaac atttgtccgg
     2581 caaacggtcc ggcgatttcg ataccggaga agacaacgcg caatccggcc agcggtccga
     2641 atttcggcat gggtagatga tccattatta gctcctgaaa aatttatgta gcgcatgact
     2701 gccggatgcg gcgtaaacgc cttatccggc ctacattcgt gctcccgtag gcctgataag
     2761 acgcgtcagc gtcgcatcag gcagcgcacg gacttagcgg tattgcttca gcaccgcacg
     2821 acccagcgtc aggatctgca tttcgtcaga tcctccggaa acgcggtcta cacgcaggtc
     2881 acgccagaag cggctgatgc ggtggttgcc cgcaatcccg acaccgccca gcacctgcat
     2941 tgcgctatcc accacttcaa atgccgcatt ggcgcagaag tatttgcaca tcgctgcatc
     3001 gccagaggtg atggtgccgt tgtctgcttt ccacgctgct tcatacagca tgtttttcat
     3061 ggagtttaat ttgatcgcca tgtgggcgaa tttttcctga atcaactgga aacgaccaat
     3121 agcctcgcca aactgcacgc gctggttggc gtagcgcgca gcatcttcaa aggcgcacat
     3181 cgccgtaccg tagttggtca gggctaccag gaaacgttca tggtcgaact cttctttgac
     3241 gcggttaaag ccgttacctt cccgaccgaa catgtctttc tcatccagtt ccacatcgtc
     3301 aaaggtgatt tcacagcagc tatccatacg cagaccgagc ttctcaagtt tggtcacttt
     3361 gatgcccggt ttgctcatat caacaaacca ttcggtgtag acaggtttgt ccggagaagc
     3421 cccgtcgcgc gccatcacca cgatgtacgg ggtgtaggcg ctactggtaa taaaacactt
     3481 actaccatta agataaatct taccatttct acgggtataa gtcgttttca ggctacccac
     3541 gtcggagccc gcgcccggtt cggtaatcgc tgagttccac atctgcttac cggtgccgcg
     3601 gaaagccata atcttgtcga tctgctcttg tgtgccttcg cgcaggaagg tattgaaccc
     3661 gcccggcagc tggtacagta cataggttgg tgcccccaga cgtcccagct ccatccacac
     3721 ggcggcgaga gtaacaaacc ccgcgtccag accaccgtgc tcttcaggga tcagcagact
     3781 gtcgataccc atatccgcca gtgctttgac aaaacgttcc gggtagacgc tgtcacggtc
     3841 gcactcggca aaataggcct cccagttttc gctggccatc agttcgcgga taccggcgac
     3901 aaacagttcc tgctcatcat ttaaattaaa atccatcttt caacctcttg atattttggg
     3961 ggttaattaa tctttccagt tctgtttcgc gtctttaata aaggagagcg tcaccataat
     4021 gttgacgaag aacagcgggc atcctccggc gataatggcg gtttgaatcg gtttcaggcc
     4081 gccgagcgcc agcagaacaa taccgataat gccaaccaga attgaccaac cgatacgcac
     4141 cagcagcggt ggttcttcac catcgcgtac ttcgcggcaa gtagacatcg ccagggtata
     4201 agagcaggcg ttaaccagcg taacggtggc aataaagcag aggatgaaga agccccacat
     4261 ggtggcggtg ctgagtggca gagcggccca ggtttcgatg atggcgcgcg ccacaccgta
     4321 ctgttcgatc agatttggaa tgttgatgat gtttttatcc atcaacagca gagtgttact
     4381 accgagcacg gtccacagga tccaggtgga tgctgtcagc cccatcacca tgccgaagca
     4441 cagttcacgc acagtacgac cacgggagat gcgggcgagg aagatactca tctggatggc
     4501 gtaaatcacc caccatgccc agtagaacac ggtccagccc tgcgggaagc cgcctttagc
     4561 gatgggatcg gtatagaaca acatgcgcgg cagatacatc agcaacatcc ccaccgaatc
     4621 ggtgaagtag ttcatgatga agctggcacc gctgacgatg aacacccaac ccagcatcag
     4681 gaagctcagg taactacgca cgtcactggc gatacgtacc cctttttgca gaccgcaagc
     4741 gacgcaaatg gcgttgagga taatccagca ggtaatgatg atagcgtcca gttgcagggt
     4801 atgcggaatg ccaaacaacc attgcataca ctcggtcacc agcggcgtgg caaggcccag
     4861 actggtaccc atcgcgaaga tcaaggcgac gagatagaag ttgtcgacga tggtaccgaa
     4921 caaccctttg gcgtgttttt cacctaccag cggcaccagt gtcgagctgg ggcgaatcac
     4981 ttccattttg cggacaaaga agaagtaagc gaaggcgact gaaaggaagc tgtaagtggc
     5041 ccacggcaga ggtccccagt ggaacaagct gtaagccagc cccaactctt tcgctcctgt
     5101 cgagttcggt tctaagccaa acggcggggt ggagatgtag tagtagatct caatcgatcc
     5161 ccagaacagt acggcagcag acgtacagga ggcgaacatc ataaagatcc aactggcggt
     5221 gctaaattct ggcggttcgt tacctaaacg ctttttggca tacgggccaa acaccagcca
     5281 gaaccaaccg aaaagcatca ccaccatata ccattcaaat
//
LOCUS       DADKXM010000198         5284 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.201, whole genome shotgun sequence.
ACCESSION   DADKXM010000198 DADKXM010000000
VERSION     DADKXM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.201"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..409
                     /locus_tag="JQR05_003788"
     CDS             <1..409
                     /locus_tag="JQR05_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5325796.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            429..740
                     /locus_tag="JQR05_003789"
     CDS             429..740
                     /locus_tag="JQR05_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5325797.1"
                     /translation="MTLTLNELLLKLDRYRVIVVDDLGYVKRDSAETGVLFELIAHRY
                     ERGSLVITSNHPFSMWGSIFVDETMAVAAADRLIHHGYMFELKGESYRKKTAKAVTSV
                     T"
     gene            complement(909..5003)
                     /gene="sepA"
                     /locus_tag="JQR05_003790"
     CDS             complement(909..5003)
                     /gene="sepA"
                     /locus_tag="JQR05_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY5325798.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSWADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1295 a         1329 c         1231 g         1429 t
ORIGIN      
        1 cgtcaggcag cagcgaagca aagcgttact gacgtcgctg aatgaatgga tggtggagaa
       61 gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg ttcagctatg tactgaatca
      121 gtgggatgcc ctctgttatt acagtgatga cggtctggcg gaggcggaca ataacacagc
      181 agaaagagcg cttcgtaccg tctgtctcgg gaaaaagaat tacatgttct tcggcagcga
      241 tcatggaggc gatcgcggag ctctgctgta cggactaatc gggagctgtc ggttgaacgg
      301 tatcgatccg gaagcgtatc tgcgccatat tctgagcgta ctgccggaat ggccctccaa
      361 ccgtgttgac gaactcctgc catggaacgt ggttctcacc aataaataag cgtcaatacg
      421 gtgctccgtt gacgcttaca ctgaatgagc tgctactgaa actggatcgc taccgggtga
      481 tagtggtgga tgatcttggc tatgtcaaac gcgacagcgc cgaaacgggt gtgctgttcg
      541 agttaatagc gcatcgctat gaacgtggga gcctggtgat aaccagtaac catccgttca
      601 gcatgtgggg cagcatcttc gtggatgaga ctatggcggt ggcggcagca gaccgactga
      661 tccatcacgg atatatgttc gaactgaaag gggaaagcta caggaaaaag acagcgaagg
      721 cagtaacaag cgtgacttga tgtcgcactg aaggggcggt cagtatagtt ggcgccagtc
      781 ggcaaaacta gttgacgtct aatacctgga cggtcacttc tgagaaaagg catttacaca
      841 gaatcttaaa caggctcacg gagttctttc agaaaaaggc ctgccgaatg gcaggcctat
      901 cccattcgtc agaaaacata acggaagttg gcgtttatcg cattatccac attatatttc
      961 ccgaacgctg atttctccag ctccagtccc aaacgcatat tatccttaat ttccgcattc
     1021 atccctaccg tcatcagcat cctgctatct ttttcacctt cgaaacgttt ctctccggaa
     1081 gcatcctgca gaaccgtttc tccgttcgcc agcaggtcga actgataacc cagcccggct
     1141 cgagctgtga ttttccagtc gtctccggag aaggctcttc ccacgtcaac accagtacgg
     1201 ccaatcagtg ggttataatc cttgtctttc atgctcagag ccattccccg gtcttcccag
     1261 ctaaaagctt ttcctgatac agaaccgtaa accagctcta tctgtggctc cacccaggac
     1321 tctttcgtca ggtggtaacg ataaccaact tcagcaccgg cataccagga atgagagctg
     1381 tagtcttttg ttcccagtga ggcaaaattc gccgtgtgct gattatcatg atggagatat
     1441 ttaccaatca ggtcaaaata agctccggaa ttaaagagtg cagaggcata cagaccgcca
     1501 cccacggatt tgttttttcc actgaatgcg tggctgcttg cattgctgtc cgtataggtc
     1561 agcaatgccc ccgtaaataa atccacaccg tccagctcat gctttctgtc gacaccaatc
     1621 tgaacgtgag tgtagttatc gctgtagtca ccatctgccg aaccggtacc attcattatg
     1681 cgtgcccaga caccggcatc cccctgagta tcccgcaggt cacccatacg tttgttcagg
     1741 ttattgacct ctgtaaggaa ggacttataa cctgatgcca taaagtcttt tgcggctttc
     1801 gaggctccgg catcagcggt agtctgatat cctgtcagca cccattttgt ggcatcatcc
     1861 gttttttccg tgctgattac tggcgtaacg ttactgaaac caatctgctg cgtaccggca
     1921 gtgaacgttt tctcatcact ccctttcggt gcggtaatta acgtcacgct catttcactg
     1981 gctggtttgt ttaaaaagtc aaccaggacg ctattattac tgcctgacag tttgtctgtg
     2041 acattcaact ggtcagccca gctgttgttg gtatgcatca ggaacgtggt attatctgca
     2101 gaaagctctt ttacttgtaa agtggcaaag tgtttcccgt cccccagttc aattcgcgag
     2161 cccttagcct tcagtgcacc ggcctctgat tttccgtcag agtaccacag agcattgatc
     2221 atatttaccg tactctgtgc ccccgtaata ctgcctctca gaacagcgtt atatcccttc
     2281 attaaggagc tgaataacgg agagtccgtc ttccctgcat cagcatcact gtctcccaga
     2341 ttaatggttg ccgcagtggt tcctgcatga atatccgaac tcagatagcc catattttta
     2401 acagaaaagc tggcgttatc ttcgaggtta atcccttctg ttgtagaaat tacgggtgag
     2461 taatacccct gtttctgcgc agaaggcatc cccgtcaggg tcagagaagc attttctttt
     2521 acatccacag ccccggtgct gaaaataccg ctctgagccg taagttttgc tccttttccg
     2581 agggtcaggc tgctgttgac aaagctgcca acacggtcaa aaacagcatt ctgagaggtc
     2641 acactcacgg tgctatcata agcctcaata ccaccggtga atctctccat gatttgcagg
     2701 gatgatttat tttccagcgt aacatttccg ctgtaatcac tctggtcacc aactgtggtt
     2761 gcagtggcac tcccttcaac agaggagacc gcattaccgg ttccgtcact cctgtcagta
     2821 aagacacggc tacttcccag aatgactgta cttccattgg tggcgttaat atctccctgc
     2881 atcttgccgt ttctggacag atggaatgtt gccgcatcga gtttcagttt ttccagcgta
     2941 aactgacggg tttcccagtc gctctggctg gaagaagtcg tcgttccagc atgaataact
     3001 ggatgaccct ggaatatcag cgtgccggac ttcttggtta tcgaaccttc tggcaggtta
     3061 actgaaccat caagagccag tacatcctta cctgagagct gcggaatatt cacatccata
     3121 ttgccgttta gttggccgtg atacatataa ggctggctgc tggcctcaat tttttgttgc
     3181 ttcacagtat caatggcttt attacgatct tttccgacat tctgccagac actgctgttg
     3241 ttcagatcgg agaagaacgg ggaatatgaa ctcgctttca ggatgaaata ttgcttggta
     3301 gaactgtcat aatataggtc tcccggcgct cctctccccc caaaaatact gacagtatta
     3361 accggtacat tgatgtcact ggcttttaat gtctgaaggt cgagtgttac cgttgatttt
     3421 ttctcactgt tattggtaat gatggccccg tagtcagccg cctgaagacg ggtaaaggtc
     3481 agattattac cattcagttc cagacgtcct ccacggtatc cccacgaaat attatccgga
     3541 ttaacttgct gtgagtcaga aagcacaact gtcggacgac cgctggcaat accaacagag
     3601 ctgaaagcct gtacctttcc gtcagcatcc gcctgctggt tgagaataac ggtaccgtcc
     3661 cccaccttga gtccgcctgc attcacaccg gtcccgttta ctgtcagggt accttcgcca
     3721 gttttatgca ggttatcacc ggcaactccg ttcacctgcc acagaacgtg ggttccttta
     3781 tcggtgataa tcccgccccc catccatgtc tggccgttca gggcggacac cctgtaatcg
     3841 ccggcaaact gcagataccc agctccctga ttaacggaat tctgcagaac gacctcgcca
     3901 ttattgccgg tgaacaggag gttttttcct gcgttcagat catttccttt ctttccatgc
     3961 atatcccaag tcgtgtttcc ctgagtcagt gtgccggtgc cattagccgc atcatatttc
     4021 cactgcagta ctccttcacc agaggtgtag gctatagttt tgtcaaaatc attttgcggt
     4081 tgctgaccaa ggaaatcctg agtggtgacg acccagttat ttccatagtt agttcctgag
     4141 gaaagcacgc caatcagtac ccattttttt tgcagggaat cataagcaaa caggggggag
     4201 ccgctgtccc ccatattcag ataattagcc agaggtccat tcgcgggatt aaaaatgtca
     4261 ccggtctgag ctgtaatcat ctgtccgttg ttataggagt tcagcgcgct gacggttccc
     4321 ccaacaagaa atccgccatt ggtatacact tgggtgcgat taccattttt gtctttgacg
     4381 tactgcatcc cccccccaag gcgatagaat tcagtaaagc gtccgccggc ctgataggca
     4441 ccgctcacag ccccgatgtc agacacttct gccggcgcca cctctgtcac cagtttactc
     4501 aggcggcggg ttttaatatc aagaccgcta ttattatttg taccaaccgc agtataggtg
     4561 tttttggcgt atccgaagct catcgtgtca gaaccgctga catgtttggc ggtaaccaca
     4621 tactgagggg agtaaagtgt atggtctccc ggaggcaggc taccggtagt aatagtggca
     4681 ctactgaaat cagccatcgg ggctttatca agtttaccga ccagattgcc ctgcttgtcg
     4741 taaatggaaa tattcgtggt ccccggcgta aactgacctt tattctcggc aaagtcgcgg
     4801 aatatctgat aagggatctc tgcactgacc gttgctgaca gagctggcca tgccgaagaa
     4861 agtgataaag ctgcaacaga cgtaagaata acccgacgtg aaagtctgcg atggcttttg
     4921 caggtaaccc tgcgggccag ttcggagaca gcaatcaggc ttttggttat atggcaatac
     4981 ttaagataat atattttgtt catatagaaa catcaaccta attttgacaa agattaatta
     5041 cataggatgt agatggagca gtccgagatt ctactaatat caattattaa gcatctgttt
     5101 tttagttggt gtaatataat ttttattaag gtaagcggaa aaacaaccaa aaaaaacgca
     5161 agatgttatt tttttatcat tttgagccag tttggctggg gggatgctct tctcttaagc
     5221 ttcttgctca actttaaaaa tagttatgcc agttcggtat cgataagggg cagtttggat
     5281 atcg
//
LOCUS       DADKXM010000199         5284 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.202, whole genome shotgun sequence.
ACCESSION   DADKXM010000199 DADKXM010000000
VERSION     DADKXM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.202"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(77..496)
                     /locus_tag="JQR05_003791"
     CDS             complement(77..496)
                     /locus_tag="JQR05_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325799.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILTSSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            546..1724
                     /gene="ltrA"
                     /locus_tag="JQR05_003792"
     CDS             546..1724
                     /gene="ltrA"
                     /locus_tag="JQR05_003792"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY5325800.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1807..1986
                     /locus_tag="JQR05_003793"
                     /pseudo
     CDS             1807..1986
                     /locus_tag="JQR05_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2016..2498)
                     /locus_tag="JQR05_003794"
                     /pseudo
     CDS             complement(2016..2498)
                     /locus_tag="JQR05_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2754..3734)
                     /locus_tag="JQR05_003795"
     CDS             complement(2754..3734)
                     /locus_tag="JQR05_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY5325801.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3734..4933)
                     /locus_tag="JQR05_003796"
     CDS             complement(3734..4933)
                     /locus_tag="JQR05_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY5325802.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVIPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
BASE COUNT         1484 a         1188 c         1238 g         1374 t
ORIGIN      
        1 gtaacgccgg ttcggaaatt gcagcataag ttatccatgc tctgaggtgg gtggaggtgg
       61 gccctccggt cagggtttac aggagcactg accaaacgac ccggtgctgc ttcattctaa
      121 ggaatgaggt ggtgagcgac tcgggaaaag gcagagatct actgtcacgt ggggttcggg
      181 aagctgaacg aaagtaaact gtccgatgaa gcatcgaaat cttagatgat gtcaaaatgg
      241 ggccgtacag ccgtcccctg ataagcgcag aagacacctg cttactgact gcgcggcatc
      301 cggtgtgaag gcagcaggat cccgggccag accatctcag ggaacgtgag aacctgtttc
      361 cggcacgtat cgggaaacag gggcggaccc attcgtagta gtgatgaaag atctgtaatg
      421 ggtctggagc gaagggatgg ggatcacatt cattgcagaa gacacaaccc aaaaggggat
      481 gacgtaactg atgcaacgaa aatcatttga aataccaaaa gcactggtct gggcatcata
      541 tctggatgtg cgtcgcaaca agggagcccc ccggatgcga cgggcagact ttgaaaatgt
      601 tcgaccagca acgtgatggc aatctataca agatctggaa ccggctatgt tcgggaacat
      661 ggtttccgcc accagtgctg gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc
      721 tggggatccc aacagtatcc gatcgaattg ctcagggagc gataaaactt ttcatggaag
      781 aaaagcttga cccgattttc catgctgatt catatggtta ccgaccaggc aaatcagctc
      841 atgacgctct gaaacaatgt gccatccggt gctggcgtta tagctggata ctggaagtgg
      901 acatcagtgc gtttttcgac catgtgagac atgatctggt gctcaaagct ctggaacacc
      961 acgggatgcc taaatgggtc atcctgtact gtcgacgctg gatggaagcg ccaatgcaga
     1021 gttgtgaaaa tggagaatta ataacccgaa cacgaggcac tccgcagggc ggggtcatta
     1081 gtccgctact tgctaacttg ttccttcact atgcatttga tttgtggatg gaaagagaat
     1141 atcggggggt accgtttgag aggtacgctg acgatattgt agtacattgt tcacgaatga
     1201 gtgatgcaac aaggctaaag aacaggttat cggagcgctt ctcggaagtc gggctggtcc
     1261 tgaatgcagg gaaaacgaat atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa
     1321 cgagtttcac ctttctcgga tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg
     1381 aacggtaccg aaaatgcatg ccaggtgcgt caaatgcagc aatgcgcaaa ataacagaaa
     1441 caatcaagaa gtggcgtata catcgctcaa cagctgagag tttgctggat tttgcgaggc
     1501 gctacaatgc gatagtgaga ggctggatcg agtactacgg aaagttctgg tccagaaatt
     1561 tcaactatcg actgtggagt gcaatgcagt cacgtctgct caagtggatg cagtctaaat
     1621 acagactttc gaaccggaag gctcagcgaa agctgacgct ggtaaggaag gagtatccga
     1681 agctatttgt tcactggtat ttactacgtg catcaaatga gtgatcaaga gccgtatgac
     1741 gcgagagtgt cacgtacggt tccgtgagca gccggggagt gaaattctct ccggctgact
     1801 cgactcgcgg gttccgacag cgatgacgaa catgcggcgg tgttgtactc gctgatcggc
     1861 acatgccgtc tgaacaatgt ggaaccagaa aaatggctgc gttacgtcat tgagcatatc
     1921 caggactggc cggcaaaccg ggtacgtgat ctgttgccct ggaaagttga tctgagctct
     1981 cagtaaatat caatacggtt ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct
     2041 ttactgaaca caatgccgta ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc
     2101 ctttccattg aaaccaaccg acgcatattc aaaaaagtct ggacgtcctg ttgctccaga
     2161 gtttttattg gtacgggtcg tatagttcca tgttggcagg cctcagctat tgcctgtgcg
     2221 tcgttataat catttttctg gcaccgctga taagctttaa catgttgtgc cggaagcaat
     2281 attacatcat ggtcgagttt cctgatttcc cgggcccagt aatgagatgc cccgccagcc
     2341 tccatcgcaa cagtcatttt aggctgttgc accagccagt taagcagttg attgcgagaa
     2401 agtttggtct ttcctgcggg tttgccatga gcattgatac tgaaaagata aaagttcgtt
     2461 ttagccagat caatgccggt gattttctgt tcagacatgg tatcccgctt acttttgtgg
     2521 ttaaagttta tccatcatgg cgcaattgaa ggctgccgtg aaagaggggg aagactatct
     2581 catcggttac tgttaaacgg ctaagagaaa ctatcttttt caagcaaaga agatacggtg
     2641 aaacaggcga tttcaccgtg aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc
     2701 ttttaaaaca gccaattaaa aactggtgag ttgctggcga aaaacttgaa ttttcacagc
     2761 gaattcagat tatcttttag tacaagagca atcatacgat ctaacttatc ctgaacttcc
     2821 aacggtaatc tgccgaattc atatgagaac gtacgtccct ttactcgttt tcgagcaaat
     2881 ttatctttac tatcaaattt ccataacaaa gttacaaccg ctttatcctt tactcctgaa
     2941 tcaagcaatg aagacatttc cttggttata agccgcataa tactgttttt aacctcgtct
     3001 tcagaaagat tgtcatcagc agtaataagc tcaatactcg gcgagatatt cttcacaact
     3061 tcatcaatag atatatcacc cagcctcagt cgttcactta attcagccag ttgcctgtaa
     3121 tcggcgtatg tcagctcaga ctgaacagga aaaagagaca caaaatcttt aggtacggac
     3181 gctgcttgga gggcacgagt caccttagct gccgacaatc cctctctttc cgcaatttgc
     3241 ttctggttca taccggcctc tttgagacga actaaacgca acccaatttc acgaatatta
     3301 tgttcaagtg acgtctgcaa atctttcgcc agatgctgag cttcagaaac agtaagctca
     3361 tcatccgtta caagtacacg taatccaacc ttgcatagca aagctgctgc ccgacgacgg
     3421 gaaccatcca aaatctcaat aagatcgcca gtccgaatcc caatacatgg atagaattgt
     3481 tgtaaacgta tagttctggt gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg
     3541 cggccattca cctcttcaac cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg
     3601 atctcagtaa attttgcctt tcggccagta ttgagggtaa atacatgaac tggagccgat
     3661 atttcttcag tattattcag gatatttgta ttcagcgtac ggccaatagt cggtcggtgc
     3721 tttctgtttt ccatcagtag ttggccctta taaattcaat acgatcaaaa actgctttag
     3781 cgaaatcttc tgctgccata cgtgcatttt tcaaagcttc accactgcca acataagtaa
     3841 caggattcgc agatatcacg gtatcaaacg actcgccgct tctctcaaaa ccatcaagac
     3901 gtggcattga aacatcaagc atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt
     3961 atttatgatc tgacttattc gccagtttcg acatgaagcc aatatttgcc tgcaaacggc
     4021 atgaacatcc atcctgttca ataatctgta caagttcagg taaacgagcc aaatatttaa
     4081 gcgtagaatg gaaatcaact tgtgctggcg gcaccggtgt aaacatgata tctgcggcag
     4141 caatggcatt tttcaaaaat gcgtccagat gcgggcctgt atcaataagg atgaagtcga
     4201 aatcatgctt cagcttatca ataatattct cacgcaatat cgcgtgtttg ttttgtccta
     4261 atagatgctc ctcgcacaga gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag
     4321 ccggtataac ataaacgcca gggataactg acggaacgat aaaatcttcc agtaattcct
     4381 cacgactcac gttctgcaac atagcctgcg gtgcggttgt atcaaccagc cccaccgcat
     4441 gcaagtagtt cagaaacata gtggctgatg actggggatc taaatcaagg agcagaatac
     4501 gtaaatcttc tgacagcagg tgaggatgag cacgcagggc gtgagctacg gaaacactac
     4561 tcacggtttt agacacaccg ccttttaaac taccgatgaa tatggaatat gcctcactat
     4621 aacgatcgcg atatttagga atgccacgat gtgcatagat atcgataatg ttctggatgg
     4681 ttaatgcata ttttgtcgcg gttcctgctg ggcgtttttc aaactgatag ccctgagcct
     4741 ccatctcacc gactgcacca tccacgcttg cgcgcgttaa ttttggaagc ttagctacag
     4801 cagactttga atagacttga taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa
     4861 tctgttttgt tagggcgagc aacattttat ctgcacgctg cgcaacctta cttaactgct
     4921 caaaactagt cattccccct ccgtgctatg taggatttta acttattaca aaaaaaactg
     4981 cattagcagg gtaatgcatg caagtacata ttgtacatgc ttatattaaa aatgcgcgca
     5041 ttgctgattt attaatcatc ggaatgaata ttactgtaag atagtatgcc tttcggggag
     5101 aaagacgctg atttcccccg aataatccag ttcaccacca cactccggac aacagctttc
     5161 ttcaggctcc agatggtgta tttcgcggga aacatgttcc ggtaacggac ggcgatgacg
     5221 tgactgtcgc aacaggcggg ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga
     5281 agga
//
LOCUS       DADKXM010000200         5190 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.203, whole genome shotgun sequence.
ACCESSION   DADKXM010000200 DADKXM010000000
VERSION     DADKXM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.203"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..273
                     /locus_tag="JQR05_003797"
     CDS             <1..273
                     /locus_tag="JQR05_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY5325803.1"
                     /translation="QFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVG
                     ATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            414..848
                     /gene="uspF"
                     /locus_tag="JQR05_003798"
     CDS             414..848
                     /gene="uspF"
                     /locus_tag="JQR05_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY5325804.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1024..1959
                     /gene="ttcA"
                     /locus_tag="JQR05_003799"
     CDS             1024..1959
                     /gene="ttcA"
                     /locus_tag="JQR05_003799"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY5325805.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JQR05_003800"
     CDS             complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JQR05_003800"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY5325806.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JQR05_003801"
     CDS             complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JQR05_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY5325807.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
BASE COUNT         1369 a         1327 c         1213 g         1281 t
ORIGIN      
        1 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
       61 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
      121 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
      181 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
      241 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
      301 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
      361 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
      421 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
      481 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
      541 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
      601 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
      661 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
      721 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
      781 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
      841 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
      901 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
      961 gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     1021 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     1081 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     1141 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     1201 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     1261 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     1321 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     1381 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     1441 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     1501 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     1561 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     1621 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     1681 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     1741 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     1801 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     1861 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     1921 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     1981 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     2041 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     2101 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     2161 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     2221 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     2281 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     2341 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     2401 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     2461 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     2521 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     2581 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     2641 tcgccgtgta acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg
     2701 caatcttttt tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac
     2761 cgttgcaaca gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt
     2821 ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg
     2881 attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc
     2941 gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat acggtcggcc
     3001 tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc
     3061 agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc
     3121 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     3181 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     3241 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     3301 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     3361 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt
     3421 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     3481 cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     3541 tcggttcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     3601 ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     3661 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     3721 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3781 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3841 tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     3901 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc
     3961 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4021 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag
     4081 gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt
     4141 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     4201 atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     4261 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     4321 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaagaatcc acgcggtgga
     4381 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     4441 aattcactgg aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg
     4501 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4561 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4621 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaaa
     4681 cggctgactc ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4741 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     4801 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4861 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4921 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     4981 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5041 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5101 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     5161 aaataacggg atctcaatga ttacgcacaa
//
LOCUS       DADKXM010000201         5112 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.204, whole genome shotgun sequence.
ACCESSION   DADKXM010000201 DADKXM010000000
VERSION     DADKXM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.204"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            139..1797
                     /gene="fliF"
                     /locus_tag="JQR05_003802"
                     /pseudo
     CDS             139..1797
                     /gene="fliF"
                     /locus_tag="JQR05_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            1790..2784
                     /gene="fliG"
                     /locus_tag="JQR05_003803"
                     /pseudo
     CDS             1790..2784
                     /gene="fliG"
                     /locus_tag="JQR05_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            2777..3463
                     /gene="fliH"
                     /locus_tag="JQR05_003804"
     CDS             2777..3463
                     /gene="fliH"
                     /locus_tag="JQR05_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY5325808.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            3463..4836
                     /gene="fliI"
                     /locus_tag="JQR05_003805"
     CDS             3463..4836
                     /gene="fliI"
                     /locus_tag="JQR05_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY5325809.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            4855..>5112
                     /gene="fliJ"
                     /locus_tag="JQR05_003806"
     CDS             4855..>5112
                     /gene="fliJ"
                     /locus_tag="JQR05_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAY5325810.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQ"
BASE COUNT         1217 a         1355 c         1498 g         1042 t
ORIGIN      
        1 aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca aataatggga gcgtcaattt
       61 ttcgagtttg ctgacccggg agtgagtctt gttccacttt gccaataacg ccgtccataa
      121 tcagccacga ggtgcgcgat gaatgcgact gcagcccaga caaaatctct tgagtgactt
      181 aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta ccggttccgc ggcagtggcg
      241 gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat
      301 ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac
      361 cgcttcagcg aagccagcgg cgctattgag gttccggcag ataaagttca cgaactgcgc
      421 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat
      481 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
      541 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgca cgtacatctg
      601 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
      661 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg
      721 gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg
      781 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
      841 gccagcgatg tcgaaggccg tattcagcgg cgtattgaaa cgatcctgtc gcctattgtt
      901 ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag taaagaacaa
      961 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
     1021 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     1081 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac
     1141 ctccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     1201 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
     1261 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     1321 cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt
     1381 tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa
     1441 agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc
     1501 ggtcgctggt tgctggtact gctggtggcg tggttgctgt ggcggaaagc ggtacgcccg
     1561 cagctaacac gtcgcgccga ggcggtgaag actgtgcagc aacaggcgca ggcccgcgag
     1621 gaagtggaag atgcggtgga agtccgtctg agcaaagacg aacaactcca acaacggcgc
     1681 gctaaccaac gactgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac
     1741 gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct
     1801 gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga
     1861 ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt
     1921 cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga
     1981 acagtttgct gcactaaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc
     2041 tctgggtgaa gaacgtgccg ccagcctgct ggaagatatt ctcgaaaccc gcgataccgc
     2101 cagcggtatt gaaacgctca actttatgga gccgcagagc gccgccgatc tgattcgcga
     2161 tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggcgccgat
     2221 attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt
     2281 ggcggcgtgc agccagccgc gctggccgaa ctgaccgaag tactgaatgg cttgctcgac
     2341 ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga aattatcaac
     2401 ctgatgaaaa cgcagcagga agaagccgtt attaccgccg tgcgtgaatt cgacggcgag
     2461 ctggcgcaga aaatcatcga cgagatgttc ctgttcgaga atctggtgga tgtcgatgat
     2521 cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa
     2581 ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat
     2641 attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa
     2701 cagaaagcga ttctgctgat tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc
     2761 agcggcgagg atacctatgt ctgataatct gccgtggaaa acctggacgc cggacgatct
     2821 cgcgccacca ccggcagagt ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga
     2881 ggccgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc acgagcaggg
     2941 ttatcaggcg gggattgccg aaggtcgcca gcaaggtcac gaacagggct atcaggaagg
     3001 actggcccag gggctggaac aaggtctggc agaggcgaag gctcaacaag cgccaattca
     3061 tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt
     3121 gatagcttcg cgcctgatgc agatggcgct ggaggcagca cgtcaggtga tcggtcagac
     3181 accaacggtg gataactcgg cactgatcaa acagatccaa cagttgttgc agcaagaacc
     3241 gttattcagc gggaaaccac agctgcgcgt acacccggat gatctgcaac gtgtggatga
     3301 tatgctcggc gcaactttaa gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca
     3361 tcctggcgga tgtaaagtct ccgccgatga aggcgatctc gacgccagtg tcgccactcg
     3421 ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact
     3481 cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt
     3541 cgctacgggc gattaacccg cgctaccggg ctggtgctgg aagccaccgg attacaattg
     3601 ccgctcggcg caacctgtgt cattgagcgc cagaacggca gcgaaacgca cgaagtagaa
     3661 agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa
     3721 ggtgtcctgc ccggcgcgcg tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc
     3781 ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa
     3841 ccgctcgatg gcctgccctt ccccgatacg acggaaaccg gtgcgctgat taccccgcca
     3901 tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc
     3961 aatgccctgc ttaccgtcgg acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt
     4021 ggtaaaagtg tgctgctggg gatgatggca cgttacaccc gcgccgatgt cattgtcgtg
     4081 ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta ttgagaatat cctcggtgcc
     4141 gaaggacgtg cacgctcagt ggtgattgcc gctccggcgg atgtttctcc gctcctgcga
     4201 atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat
     4261 gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg
     4321 gcgattggcg aaccacctgc cactaaaggt tatccaccgt cggtgtttgc caaattaccg
     4381 gcactggtcg agcgtgccgg taatggcatt agcggcggcg gctcgattac cgcgttttat
     4441 accgtgctca ccgaaggcga tgaccagcag gaccccattg ctgactccgc gcgggcaatc
     4501 ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc
     4561 gatattgaag cgtcgatcag tcgcgcaatg acggcgttga tcagtgagca acattacgcg
     4621 cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc
     4681 gtcggcgcgt atgccaaagg cagcgatccg atgctcgata aagccatcgc cctgtggccg
     4741 cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc
     4801 caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggcg
     4861 gaacatggtg cgctggcgac cctgaaagat ctggcagaaa aagaggtaga ggatgccgct
     4921 cgcctgctgg gtgaaatgcg tcgcggatgt cagcaggcgg aagaacagct caaaatgctg
     4981 atcgattatc agaatgaata tcgcaataac ctcaatagcg atatgagtgc cgggatgacc
     5041 agcaaccgct ggatcaacta tcagcagttt atccagacgc tggaaaaagc cattactcag
     5101 catcgccagc aa
//
LOCUS       DADKXM010000202         5044 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.205, whole genome shotgun sequence.
ACCESSION   DADKXM010000202 DADKXM010000000
VERSION     DADKXM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.205"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JQR05_003807"
     CDS             complement(<1..209)
                     /locus_tag="JQR05_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HAY5325811.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPA"
     gene            complement(318..731)
                     /gene="hiuH"
                     /locus_tag="JQR05_003808"
     CDS             complement(318..731)
                     /gene="hiuH"
                     /locus_tag="JQR05_003808"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY5325812.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            864..1535
                     /gene="hprR"
                     /locus_tag="JQR05_003809"
     CDS             864..1535
                     /gene="hprR"
                     /locus_tag="JQR05_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY5325813.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1535..2893
                     /locus_tag="JQR05_003810"
     CDS             1535..2893
                     /locus_tag="JQR05_003810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY5325814.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYKKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(3001..3852)
                     /gene="hchA"
                     /locus_tag="JQR05_003811"
     CDS             complement(3001..3852)
                     /gene="hchA"
                     /locus_tag="JQR05_003811"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY5325815.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLLRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(4452..4631)
                     /locus_tag="JQR05_003812"
                     /pseudo
     CDS             complement(4452..4631)
                     /locus_tag="JQR05_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4725..>5044
                     /locus_tag="JQR05_003813"
     CDS             4725..>5044
                     /locus_tag="JQR05_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325816.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRA"
BASE COUNT         1420 a          998 c         1192 g         1434 t
ORIGIN      
        1 gcaggcttgc tgaactccag cggttttccg gcgggggcgg gcggacgatc gttcccttta
       61 aaccagctaa gcagatcggc atgcgcagcg tgaggcaaag aaaatgcagc tgcgctgatg
      121 cccagtgctt ttaacacctg ccgacgcttc ataaagaata ccgactcggc cgtaacatct
      181 gattctttta aaaattgatt ctttttcata agccatcaca cttttttgtc gttaaccaac
      241 aagagctgtt tactgttaat ctgtcaggga gtcacctccc taacagtagc agagatcagc
      301 ttttacatat ttacgcatta actgccacga taggttgaat acccatattg gctgagtaac
      361 aaaggcacat gataatgctc gttcacttta ttaatatgaa attcaacggg aatctcaggg
      421 aagaaacttt caagattttg tttcttgaaa tagtccccgg ttttaaatac gacacggtaa
      481 tcgccagtag tcgcagtttg ctcgggccac agtgccttaa ttcgtccatc cttatctgtt
      541 ttggcggtat taagttgtaa ccagccgtta tccgctttct tttcaagagt gactgtcacg
      601 tcggcagcgg gttttcctgt ttgctggttc aaaatgtgca cgctaagaat gttttgttgt
      661 gcggcataaa ccaaagaagg taatgaaaat gctgccgttg ctaccgagag tactaaataa
      721 cgctttaaca tgtttatatc cttgtcatgt gaatgagttt tgaggatagc gaaaaaacgt
      781 ccagggtaac cttacagcag cattacaatt ttgtaatgaa tcaggttgtt tctataacat
      841 atgatttatg gcatattatt ttcatgaaga ttctacttat tgaagataat caaaggaccc
      901 aggaatgggt aacgcagggg ctttccgaag cgggttatgt cattgatgcc gtttctgatg
      961 gcagagatgg gctttatctt gcgctgaagg atgattatgc attgatcatt ctggatatta
     1021 tgcttccggg tatggatggc tggcagatct tacaaacgtt aagaacggca aagcaaaccc
     1081 ctgttatttg ccttactgca agggattctg tcgatgacag agtcagaggg ttggacagtg
     1141 gggcaaatga ttatctggta aaaccttttt cattttctga gttgctagca agggttcggg
     1201 cacaattaag gcaacatcac gctctgaatt caacattaga aatcagtggc ttaaaaatgg
     1261 actctgtcag tcaaagtgtg agcagggaca atatcagtat tacactgacg cgcaaggagt
     1321 ttcagttact ttggctactg gcctccagag ctggcgagat tatacccaga acggttattg
     1381 cgagtgaaat ttggggaatc aactttgata gtgataccaa tacggtggat gtcgccattc
     1441 gcaggctccg cgcaaaagtt gatgatcctt ttcctgaaaa gctaatcgcc acaatccggg
     1501 ggatgggcta ttcattcgta gcggtaaaaa aataatgaaa agattatcta taaccgtccg
     1561 tttaaccttg ctttttatat ttctgcagtc tgttgctggc gctggaattg tatggactct
     1621 ctataatggc ctggcaagtg agttgaaatg gcgcgatgat acaacactca ttaaccggac
     1681 agcgcagatc aagcagttgt taattgatgg ggtaaatcca gatacgttac ctgtgtactt
     1741 taaccggatg atggatgtta gtcaggatat tttgatcatt catagtgatg gcatcaataa
     1801 aattgttaac cggacaaatg tcagtgatga catgttaaat aacatacctg ctagtgagac
     1861 aatcagcgca gcaggcattt acagaagcat tattaatgat acagagatag atgctttacg
     1921 aattaacatt gatgaagttt cgccatcatt aacggttact gtggctaaat tggcttcagc
     1981 cagacataat atgcttgaac agtataaaat taatagcatt ataatttgca ttgtcgccat
     2041 tatactgtgc tcagtattaa gtccgttgtt aatcaggacg gggttgcgag agatcaaaaa
     2101 gttgagtggc gtaacggaag cgctgaatta taacgatagc cgagtgccgg ttgaggttaa
     2161 cgcattaccg agagaactaa aacctcttgg gcaggcgttg aataaaatgc atcatgcttt
     2221 agtcaaagat tttgagcgcc ttagtcaatt tgctgacgat ctcgctcatg aacttagaac
     2281 gccaattaat gcattactgg gtcagaatca ggttacgctc agtcaaacca gaagtatcgc
     2341 tgaatataaa aaaacaattg ccggaaacat tgaagagctg gaaaatattt cgcggttaac
     2401 agagaatata ctgtttcttg ccagggcaga caaaaacaat gttttggtga aactggattc
     2461 gctttctctc aataaggaag ttgaaaatct tttggactat cttgaatatc tttcagacga
     2521 gaaagagatt tgctttaagg tcaagtgcaa tcaacaaatc tttgcggata aaattttact
     2581 gcaacgaatg ttatcgaatc ttattgttaa cgccattcga tattcaccag aaaaatcgcg
     2641 tattcatata accagttttc ttgatgccaa cggctctctt aatattgata tcgccagtcc
     2701 tggaacgaaa attaatgagc ctgaaaaact cttccgtaga ttttggcggg gagataattc
     2761 gcgtcattcc gtaggtcagg gacttggcct ttctttagtc aaagcgattg ccgaattaca
     2821 tggaggaagt gctacgtatc actatctcag taagcataac gtgttccgaa ttacgttacc
     2881 gcaaagaaat taacatcggg cttcagtacg ccataagtta accgtgaaaa aacgcagcca
     2941 cgtcgtgagt attaacgtgg ctgcgattga ttatgcgctt acatcacaat gtaacaggga
     3001 ttaactcgcg taagctgcca gcatttcctg cgccgccagt ttacccaacg cattcgctgc
     3061 aaaaggactg tcgccggtga gaagtttacg gtccttatgt actcgcccgg tgatgtcgtc
     3121 attaatgatg ttcatgccca ttttcttcaa ctcttcgccg aagtaccagg tgagatgacc
     3181 cggcatatag ccaatatctg gcgtttgttt gtctgcggcg tctgggaatg cgcaaataga
     3241 ataaccattt agtgggttat cgctgtggcg aagtgccaga aaagccgccg gaccgtggca
     3301 aagggagata acaaaacggt catttttgat tgcccactgt aaagcggcag ccacgtcctg
     3361 gctttctggt aaaccaataa gtgcgccatg cccaccagga acaaagattg ctgcatattc
     3421 gctatcagcg ttgaggctgg cgacaacatc cgcgagtttc ttcggattac gtaataacga
     3481 tttatgctgt tcaaagaatg gcattacttt ttcatctttg tgcggcatag cccagtattc
     3541 aaacttggtc atcagaccgg aaatggtcgc cacttcgaat tcgaaacctg cagcatggag
     3601 atgatacaac ggcagcaacg tttcaatcgg atggttaccg gtcgagaaca gttttccgtt
     3661 atcggtcggc aaataacgtt cgtccgcggc gatcaccaga attttatgtt taccgcgata
     3721 cggttttgga tagtccacgc catcaagatc agagacggga ctcgtatatt ggctaagcga
     3781 atattctgaa gggaagaacg cattatcttc agcaatatcg acctgcgggt ttttacttgt
     3841 ttgaacagtc atagtgtatt ccttattgtt gcttagttag ggtaatcact atatttgcga
     3901 ctgagctggt cgctattttt aaccaggctg aaaaattgag tcaatatatc gaaagcggta
     3961 gttggcgggc ataagtatgt aatgatgatg ctgcactggt gttgaatatt attcacgcca
     4021 aactttcctc attgagcaat gtcgtcgcaa gcgcaagtgg cctcaatgat tttagaccat
     4081 tatcagaggt ccggagcggc gggaagagat ttattgctgt ggaataattg tggtactgat
     4141 gattatttat atcccggatt ggtagggaaa agcttttttc atgcactatt tttgcaaata
     4201 tttctatatg ggaagcggtt ttgttacaga ttaaatttat aaagaaatgc atttggggtg
     4261 ttgggttgaa gagtagctgg tagatgttat tgtgccagca ggggcagggg aatgtgccgt
     4321 atcgatatgc tttggcaatt gcgtgtgccg cagatggaga tgaaatgcca ataaaaaagc
     4381 ctgagttcga tgagggacga actcaggctg gtgttatgtt ttacagtaac acgcctcggc
     4441 gcgttggctg attagaactg gtagacgata ccaactgcca gttggtcgtc ggtgttcagt
     4501 ccgtgagcga gaacataatc actcttatca agcaggttga tctgataagc ggtatatatg
     4561 ttcatattct tattgaagta ataccaggta ccgatttcaa tatagttcag acgatcagaa
     4621 tctttgtaac ctgtagactg gccccctgaa tctccagaca accaatatca cttatttaag
     4681 tgatagtctt aatactagtt tttagactag tcattggaga gcagatgatt gatgtcttag
     4741 gaccggagaa acgcagacgg cgtactacac aggaaaagat cgctattgtt cagcagagtt
     4801 ttgaaccggg aatgacggtc tcccttgttg cccggcaaca cggtgtggca gccagccagt
     4861 tatttctctg gcgcaagcaa taccaggagg gaagtcttac tgctgtggcc gccggagaac
     4921 aggtcgttcc tgcctctgaa cttgctgctg ccatgaagca gattaaagaa ctccagcgcc
     4981 tgctcggcaa aaaaacgatg gaaaatgaac tccttaaaga agccgttgaa tatgggcgtg
     5041 caaa
//
LOCUS       DADKXM010000203         5028 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.206, whole genome shotgun sequence.
ACCESSION   DADKXM010000203 DADKXM010000000
VERSION     DADKXM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.206"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..836
                     /locus_tag="JQR05_003814"
     CDS             <1..836
                     /locus_tag="JQR05_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5325817.1"
                     /translation="RNELLHGETLYLDNPRSNKKGRTVRTVDNYIALLCSLLRFAYQS
                     GFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELA
                     ALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRH
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLDARL
                     S"
     gene            1323..2213
                     /gene="sitA"
                     /locus_tag="JQR05_003815"
     CDS             1323..2213
                     /gene="sitA"
                     /locus_tag="JQR05_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY5325818.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDMVKKNHIPAVFSESTISDNPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            2213..3040
                     /gene="sitB"
                     /locus_tag="JQR05_003816"
     CDS             2213..3040
                     /gene="sitB"
                     /locus_tag="JQR05_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY5325819.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            3037..3894
                     /gene="sitC"
                     /locus_tag="JQR05_003817"
     CDS             3037..3894
                     /gene="sitC"
                     /locus_tag="JQR05_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY5325820.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            3891..4748
                     /gene="sitD"
                     /locus_tag="JQR05_003818"
     CDS             3891..4748
                     /gene="sitD"
                     /locus_tag="JQR05_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY5325821.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1288 a         1186 c         1221 g         1333 t
ORIGIN      
        1 accggaatga actgctgcat ggagaaacgc tttacctaga taatccaaga tccaacaaaa
       61 aaggaagaac cgtgcgcaca gttgataact atatcgccct gctctgttcg ttgttacgtt
      121 ttgcgtatca gtcgggattt atatcaacca aaccatttga aggagtaaaa aaattacagc
      181 gaaacagaat aaagcctgac ccgttatcta aaacagaatt caatgcatta atggaaagtg
      241 aaaaaggaca gagccaaaac ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg
      301 aactggcagc tctggcgtgg gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa
      361 gaaacctgac gatacttgat atgttcggtc ccccaaaaac aaatgccggg atccggacgg
      421 taacattact gcagccggct cttgaagcac tgaaggagca atacaaactg accgggcatc
      481 atcgcaaaag cgaaatcact ttttatcatc gggagtacgg cagaactgaa aagcaaaaac
      541 tgcattttgt tttcatgccc agggtgtgta acgaaaaaca gaaaccttat tactcggtaa
      601 gcagtttggg tgcaagatgg aatgcagcag taaaacgtgc tggtattcgc cgccataatc
      661 cgtaccatac gcgacatact tttgcctgct ggctgttgac ggcaggagcg aacccggcat
      721 ttatagccag ccagatgggg catgaaactg cgcaaatggt gtatgaaatt tacggtatgt
      781 ggattgatga catgaacgac gaacagatag ccatgttgga tgcgcggtta tcgtagttgc
      841 aaagtttgcc cccaatttgc cccatttagt accagagaac tgaaataatg caagaaaatc
      901 aacaaattac aaagaaagaa caatacaacc tgaacaagta agggcaaaaa tcacaactat
      961 ctgactcata agtattttat ttatttttca atatgttaaa cattatcagc gacaacaata
     1021 agctacgata acccactctt tttttgcccc ttttttgccc cttctacagc gttttgcccc
     1081 atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag ctgctctact
     1141 tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt aggcctcaaa
     1201 gctgttaatg ataattattt tcattttgta taggttatgc tataaaacat agccgttgct
     1261 atgtttgttc taattttgct cactataggt actaaattat gcactcaata aaaaaagtaa
     1321 ccatgctctt ggggtgcctc gccctcacct gctcgatcgc atttcaggca agtgcaactg
     1381 aaaaattcaa ggtcattaca acatttacca tcatcgcgga tatggccaaa aacgtggctg
     1441 gagatgctgc agaagtctca tccataacca agcctggtgc agaaattcat gagtatcagc
     1501 ctacccctgg cgatattaaa cgtgcgcagg gggcacaact gattctcgcc aatggtatga
     1561 atctggaatt gtggttccaa cgcttttacc agcatctcaa tggggttcca gaagtaattg
     1621 tctcttcggg tgtgacgcca gtagggatca ccgaaggacc ctatgagggc aaacctaatc
     1681 cccatgcatg gatgtcgcca gataatgctc tgatttacgt cgataatatt cgtgatgcgt
     1741 tgataaaata cgatccggca aatgcacaaa cctaccaacg taatgccgat acttataaag
     1801 ccaagattac ccaaaccctt gcccccctgc gtaagcagat tacggaactc cctgagaatc
     1861 agcgatggat ggtcaccagt gaaggggctt tttcttatct cgcacgcgat ttggggctaa
     1921 aagagcttta tctgtggccg attaatgccg atcaacaagg aacaccgcag caggtacgta
     1981 aggttgttga tatggttaag aaaaatcata tcccggcagt ctttagcgag agtacgattt
     2041 ccgataatcc agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc ggtgtgctct
     2101 atgtcgattc cctgagcaca gaaaacggcc cggtacctac gtatatcgac cttcttaagg
     2161 ttactaccag tacgctggta cagggaatta aagccgggaa aagggagaaa taatgatgca
     2221 atctgcaggc attgtcgtta atgatgttac tgtcacctgg cgtaacgggc acacagcact
     2281 gcgcgatgca tccttcacag taccaagtgg atctattgcc gctctggttg gggtaaacgg
     2341 ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga ccagcggaaa
     2401 gatatctgtt ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg ttgcctacgt
     2461 ccctcagtca gaagaggttg actggtcatt tcctgtactg gtggaagatg tggtgatgat
     2521 gggccgctat gggcatatgg gtatgttgcg tatagccaaa aagagggatc gtcagattgt
     2581 caccgatgcg ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa tcggcgagct
     2641 ttccggtgga caaaagaaac gcgtctttct ggcgagagcg attgcacagc agggtgatgt
     2701 gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga aaatcatcag
     2761 cctgttgagg gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc acaatctcgg
     2821 ctcagtcacg acattttgcg attatacggt catggtcaaa ggcaccgtcc tggcaagcgg
     2881 gccgacagac accactttta cagccgagaa cctggagctg gcttttagcg gcgtactgcg
     2941 ccatgtcaca cttaacggct cggaagaaag cattattact gacgatgaac gcccttttgt
     3001 ggcacatcga ccgtcagcgg tgcagaggga agcaagatga acgtgctact ggaacccttc
     3061 agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg tctctgcgct
     3121 ttcctgtcat gctatctgat gctaaaaggc tggtcgctga ttggcgatgc gctgtcgcac
     3181 tctattgtcc cgggtgttgc gggggcatat atgctggggc ttccgttttc tctgggagcc
     3241 ttcttttccg gcggactggc agcgggcagc atgctgtttc ttaaccaacg cacccgctta
     3301 aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg gctgtttatg
     3361 gtgtcattga acccgacatc cgtgaacatt cagaccatcg tcctgggcaa tattctggca
     3421 atcgatccgg cggatatcct gcaactcact atcattggca ttctctcgat catcgtgctt
     3481 tttttcaaat ggaaagatct tatggtgacc tttttcgacg agaatcacgc ccgtgctatt
     3541 ggattacatc cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt ttcgaccgtt
     3601 gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc tggtggtgac tcccggtgca
     3661 acagcatggt tgttaaccga tcgttttccc cgcctgctca taattgctgt aactattggc
     3721 agtgtaacca gtttcctcgg cgcgtgggtc agttactttc tggacggcgc gacaggtgga
     3781 atcatcgtgg tggcgcagac actgctgttt cttttagcat ttgtatttgc gcccacccat
     3841 ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc atgatggcat
     3901 tattactgga gccactgcag ttcaccttta tgagtcacgc actgctgatt tcactggtag
     3961 tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg gcactgatgg
     4021 gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct tacctggatt ctggggttac
     4081 ctctggctac aggggcattc gtcgccggag tattctgcgc ggtggcgacg ggatacctga
     4141 aagacaacag ccgaatcaag caagatacag ttatggggat tgttttttct ggcatgtttg
     4201 ccgcaggtct gatcttgtat attgcaatta aaccagacgt acatcttgac catattcttt
     4261 tcggcgacat gctcgggata accatcggcg atataatcca gacggtgatt attgccgggc
     4321 tggttacact tgttattagc gtaaaatggc gggatttttt gctgttcagc tttgattatc
     4381 aacaggcgca ggtaagcggt ttgcatacga gatggctgca ttatgggctg ctgtgcatgg
     4441 tttccctgac cattgtggcg acgctgaaag cagtaggtat cattctctct atttctctgc
     4501 tcattgcgcc tggcgccatt gcagtactcc tgacacaacg ctttcatatt gcgctcctac
     4561 tggcgactgg catatcggta atagtgtcaa tgactggtgt ctggctgtcc ttttttatcg
     4621 acagcgcacc agctccgacg attgtcgtct tattcgcggt cgtgttcatc atgacgttta
     4681 ccgttaccag catcaacgca cgcacaaagg gaaacgccca tacacaggat ctgttatcac
     4741 ccaattagca caacatccga ggacagcatc gtccgccagc ctacagaagt ccggaaagcc
     4801 atggaaggtc gttgggagga ggtacgaatt ctatcatgca aaaaatacgt aaaatcgata
     4861 acgcctagaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcggcacgt
     4921 tcctgcatac gacgtgccgc agcttctgtt attccacgat aaaattccta accgaattca
     4981 aatccttcag actattcagc tcttccttca gctcccgctg acggcgat
//
LOCUS       DADKXM010000204         4993 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.207, whole genome shotgun sequence.
ACCESSION   DADKXM010000204 DADKXM010000000
VERSION     DADKXM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.207"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JQR05_003819"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JQR05_003819"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQR05_003820"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQR05_003820"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY5325822.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQR05_003821"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQR05_003821"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY5325823.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADCNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQR05_003822"
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQR05_003822"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY5325824.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3361..4695)
                     /locus_tag="JQR05_003823"
     CDS             complement(3361..4695)
                     /locus_tag="JQR05_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325825.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1204 a         1418 c         1186 g         1185 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 cgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgcca acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacatcag caatagcttc tgggttacag tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt tagtcggcac
     3121 tcgcactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aaccaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 agcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aatatcattg tgcaaatgct aatttaatta atacttttta aatattattt
     4861 tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccgggc
     4921 ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa actcctgaca
     4981 ttcttatttc acc
//
LOCUS       DADKXM010000205         4820 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.208, whole genome shotgun sequence.
ACCESSION   DADKXM010000205 DADKXM010000000
VERSION     DADKXM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.208"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1103)
                     /locus_tag="JQR05_003824"
     CDS             complement(<1..1103)
                     /locus_tag="JQR05_003824"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5325826.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMELSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JQR05_003825"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JQR05_003825"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY5325827.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..4765
                     /gene="treF"
                     /locus_tag="JQR05_003826"
     CDS             3116..4765
                     /gene="treF"
                     /locus_tag="JQR05_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY5325828.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPAFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGKP"
BASE COUNT         1220 a         1246 c         1158 g         1196 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aactccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttg
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gcttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     4381 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     4441 gttactgaca cctggcggga ttttggcaag cgagtacgaa accggtgaac agtgggataa
     4501 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     4561 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     4621 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     4681 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     4741 aattggtttg tacggcaaac cataatattt ttacagccag ccgctaactt cctgctggct
     4801 gtaaaattat cctcttcagg
//
LOCUS       DADKXM010000206         4666 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.209, whole genome shotgun sequence.
ACCESSION   DADKXM010000206 DADKXM010000000
VERSION     DADKXM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.209"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            39..773
                     /locus_tag="JQR05_003827"
     CDS             39..773
                     /locus_tag="JQR05_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY5325829.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYNLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(775..1386)
                     /gene="emtA"
                     /locus_tag="JQR05_003828"
     CDS             complement(775..1386)
                     /gene="emtA"
                     /locus_tag="JQR05_003828"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY5325830.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1486..2400
                     /gene="ldcA"
                     /locus_tag="JQR05_003829"
     CDS             1486..2400
                     /gene="ldcA"
                     /locus_tag="JQR05_003829"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY5325831.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2495..4231
                     /locus_tag="JQR05_003830"
     CDS             2495..4231
                     /locus_tag="JQR05_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY5325832.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT         1041 a         1191 c         1196 g         1238 t
ORIGIN      
        1 taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac catgagcagt
       61 tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa
      121 ttcctttgcg tctcagttgg aatggcgggc agctgatcag caaaatactg gcaataaccc
      181 cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg
      241 cacagcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt actgttgaac
      301 aactacagca gagtgaatac ttgcagcttc cggcatttat taccgtaccg cctcccacct
      361 tatggtttgt acaacgacgc cgatatttcc gcatctccgc ccctctccat ccgccttatt
      421 tttgccagac caaactggcg gataacagta cgttacgttt ccgcttgtat aatttgtcgt
      481 taggcggcat gggcgcatta ctggaaacag caaagcctgc cgaattacaa gaaggtatgc
      541 gcttcgctca aattgaagtc aacatggggc aatggggtgt ttttcacttt gacgcccagt
      601 taatctccac cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc accactcccc
      661 gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta cagcggatta
      721 ttttctctct cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac tgatttacat
      781 cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg caggatgatt
      841 tcgcgctacg tgatcgagga actcgtcagc atccaaatcg ttgattttgc tgatcgcctt
      901 tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt atgacaccac
      961 cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg tttccagaat
     1021 attcaggtaa gccgccccca ttgaaatatt acgctctgga tttttcagct cgctggtcgt
     1081 cggctcacca ctccagccca tacggcgata aacatcacgt ccggaggttg aagcttttaa
     1141 ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac ccgattcgat
     1201 agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg ctttctggct
     1261 tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg gcgggttcgt
     1321 atagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa accatctcaa
     1381 tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg gcacacctaa
     1441 gcggcatgat atgcatttcg tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac
     1501 ttaattgccc catcgggtta cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc
     1561 cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt
     1621 tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag actgactacc
     1681 cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt actggcagat
     1741 attgactggc aggcactggt ggctcgccaa caacatgacc cgttactcat ttgcggacat
     1801 agcgatttta ccgccattca gtgtggtctt ctggcgcaag gcaatgtcat cacctttagc
     1861 ggccccatgc tggtagcaaa ctttggtgcg gatgaactga acgcctttac cgagcaccat
     1921 ttctggctgg cgttacgcaa tgaaaccttc accattgaat ggcaagggga aggcccgaca
     1981 tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc actgattggt
     2041 acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat taacgagcac
     2101 cctttccgcg tcgaacgtat gctgttgcag ctctatcatg cgggaatttt gccacgtcag
     2161 aaggcgatta ttctcggtag ctttagcggc agcacgccca atgattatga cgcaggttac
     2221 aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt cgattccact cattaccggc
     2281 ctcgattttg gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca tgccattctg
     2341 aataacaccc gggaaggcac tcagttgacg attagcggtc atcctgttct taaaatgtaa
     2401 aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg gtagggttta
     2461 tataagagac agcgtaatca ggagtaaccg accgttggat gccacaacaa taattagcct
     2521 tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat tttcttcccg
     2581 tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg gagtcgatgg
     2641 cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta acctggcgct
     2701 ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt
     2761 aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt taaccggcat
     2821 gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg gcgctatcgt
     2881 cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg
     2941 tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg tctttctgac
     3001 gattaccctg attgcaatga tccagcagca tgaaagcagt gtcagttgga tgttcgtggt
     3061 cgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg gttatttact
     3121 gctgcaaatg attaatcgca tcgccctgcc tgccggatta tatccattgc tggcattaag
     3181 cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggcagcggta ttctggctgt
     3241 ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg gcatcctgca
     3301 aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc tggggctatt
     3361 ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt cggcatggat
     3421 gatattcttc gcccgtcccc tttcggtatt tgctggattg ctccctttcc gcggtttcaa
     3481 tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc caatcatcct
     3541 ggccgtgttc ccgatgatgg cggggctgga gaatgcgcga ctgttcttta atgtcgcctt
     3601 ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa
     3661 agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc tggatattca
     3721 tccggaaaat ccgtgggagc agtttgttta tcaattgagt gctgataaat ggtgtgtagg
     3781 tgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc tgtttcgtga
     3841 taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt
     3901 aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt cgccaccggt
     3961 cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca aatatgctga
     4021 tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac agcaaacgct
     4081 gggtgaaatt gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt
     4141 tgccgggatg atctggacgg tggccgagaa agaagataat gaagtgttga agattggcgt
     4201 tcgggtggct gaagaggaag ctgaatctta aatttaatgc agcatctatc gccggatgcg
     4261 atgctttact gaacgcagcc tgagcctgta ataaacttgt catttcctga ttttgatatg
     4321 ctcatcccaa catcagaaga aggttaaatt atggatgaca aaccattgca ggctctggct
     4381 aatgaactca aaacaccggc tcaagaaagt cagcgtaagg gctttttccg actgtaacag
     4441 gtaataaaaa ccagccccgc attaccggtc aaccatcagg ggcccctgcc tgacatcgcc
     4501 tgaacatatt cccccacgca acctgccgca ctgacatagc ccacacaata ggcgtttcat
     4561 ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc ttaataacga
     4621 ttatttccgg gtcgttcact ctcgtatttt ctttaatacg tcagag
//
LOCUS       DADKXM010000207         4653 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.210, whole genome shotgun sequence.
ACCESSION   DADKXM010000207 DADKXM010000000
VERSION     DADKXM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.210"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            64..249
                     /locus_tag="JQR05_003831"
                     /pseudo
     CDS             64..249
                     /locus_tag="JQR05_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            391..612
                     /locus_tag="JQR05_003832"
                     /pseudo
     CDS             391..612
                     /locus_tag="JQR05_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(633..695)
                     /locus_tag="JQR05_003833"
                     /pseudo
     CDS             complement(633..695)
                     /locus_tag="JQR05_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1146..2558
                     /gene="ospC1"
                     /locus_tag="JQR05_003834"
     CDS             1146..2558
                     /gene="ospC1"
                     /locus_tag="JQR05_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY5325833.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2662..2811)
                     /locus_tag="JQR05_003835"
                     /pseudo
     CDS             complement(2662..2811)
                     /locus_tag="JQR05_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2887..4584
                     /gene="ospD3"
                     /locus_tag="JQR05_003836"
     CDS             2887..4584
                     /gene="ospD3"
                     /locus_tag="JQR05_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY5325834.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFAENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1622 a          839 c          865 g         1327 t
ORIGIN      
        1 cctgttttta taacggtaat tgcagaccat gacaaaccgc agccgtcagg ctgcctactc
       61 gagtcacaag gctctctgtg tccgatatgc cggcagcgca ttacccatga aactggctgg
      121 aacgtacacc ataaggtgaa gaaagttatg gggggcggtg aagaattatc taaccttgtc
      181 ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag
      241 ggcttataaa ggcttgaggg tgtaccggga aaccggtatg caccgttctt aggggagggc
      301 agtccagcaa tgaactgtcc ttacccgaca aacatccaaa acgaacggaa cgtgctgaaa
      361 tccgcatata acccgttgta aagtgaggaa atggacacct cacttgctca tgagaacgcc
      421 cgcctgcggg cactgttgca gacgcaacag gacactatcc accagatggc tgaatacaac
      481 cgcctgctct cacagcggat ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg
      541 gttgcgaaac tgcagcgtat gcagttcgat aaaagctcag aaaaacttcg cgcaaaaacc
      601 gaacggcaga ttccattttc acgggcgatc tacgccacac aggctctggg ttgcagtgac
      661 tgctccatta aggcgatttt gaactcataa gggaagtaac aaaaaaactg caaaagtaca
      721 ataattatat atggccaaac taacctctat taaaaaaaca tcagtttttc acacttagtt
      781 gagcttcatg aaaaaatcat cgagggtttc attgcagaat acacattcca ctaactggaa
      841 gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt
      901 caataaaatt gatatagtta ttaaattatg aaaataattt cacaaaaaag attaacttga
      961 gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt
     1021 atataatacc cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt
     1081 cataccctgc tatatcttgt agggaaagat attaattaaa actgttttca tataaggctc
     1141 attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta
     1201 tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac
     1261 aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa gatttcttta
     1321 ggaaaactat agcagcacag agttatagta gaatgttctc tcaaggctct aactttaaat
     1381 ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc
     1441 accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt
     1501 ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc
     1561 aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt
     1621 ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc
     1681 atgactttgt gttttttggc gttgaaatct ctagccatca gaaaaaacac cccctgaata
     1741 caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat gactctccat
     1801 atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg
     1861 agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac
     1921 atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc
     1981 tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt
     2041 gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc
     2101 cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag
     2161 aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca
     2221 ctaacaaaaa aattgctctc caggctcttt ttctttcgat tactaatcaa aaagaggatg
     2281 tcgccttata tatattatct aattttgaga taactagaca agatgttatt tccataaagc
     2341 atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg
     2401 agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg
     2461 gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat
     2521 tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc
     2581 aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg
     2641 ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga gctggaaggt
     2701 cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat
     2761 acccccctga gtttcagagt aacggggaca aaccacatca atctttgcca tcaatcatcc
     2821 aaagggcaaa gagtacaaca acactaagtc tgcgtcacaa cccatcaatg aaaggaatat
     2881 atacatatgc catcagtaaa tttaatccca tcaaggaaaa tatgtttgca aaatatgata
     2941 aataaagaca acgtctctgt tgagacaatc cagtctctat tgcactcaaa acaattgcca
     3001 tatttttctg acaagaggag ttttttatta aatctaaatt gccaagttac cgatcactct
     3061 ggaagactta ttgtctgtcg acatttagct tcctactgga tagcacagtt taacaaaagt
     3121 agtggtcacg tggattatca tcactttgct tttccggatg aaattaaaaa ttatgtttca
     3181 gtgagtgaag aagaaaaggc tattaatgtg cctgctatta tttattttgc tgaaaacggt
     3241 tcatggggag atattatttt ttatattttc aatgaaatga tttttcattc cgaaaaaagc
     3301 agagcactag aaataagtac atcaaatcac aatatggcat taggcttgaa gattaaagaa
     3361 actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat
     3421 ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat
     3481 tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat gtctatattt
     3541 gtggacagac atactccaac aagcatgtcc tccataatca gatggcctaa taatttactt
     3601 caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta
     3661 acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc
     3721 aaaaatgacg atggtttttc aggattgctt ttagctttac aaaatgggca ttcagataca
     3781 atcttagcat acggagaact cctggaaact tctggactta accttgataa aacggtagaa
     3841 ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat
     3901 gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc
     3961 gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag
     4021 atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt
     4081 gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca
     4141 aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca
     4201 atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag
     4261 ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga
     4321 tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat
     4381 caggcatcaa aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct
     4441 tttcaaaata ggaattttga ggcaataaaa acttatatgg gaataataaa aaatgaaaat
     4501 attacacctg aagaaatagc agaacacttg gacaaaaaaa atggaagtga ttttctagaa
     4561 attatgaaga atataaaaag ctgaatatta tctcgtggtg atacactgaa tttgaggtaa
     4621 ccggctcatt taaaccgtct ggtctgttac ctc
//
LOCUS       DADKXM010000208         4646 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.211, whole genome shotgun sequence.
ACCESSION   DADKXM010000208 DADKXM010000000
VERSION     DADKXM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.211"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(51..812)
                     /gene="kduD"
                     /locus_tag="JQR05_003837"
     CDS             complement(51..812)
                     /gene="kduD"
                     /locus_tag="JQR05_003837"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY5325835.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(842..1678)
                     /gene="kduI"
                     /locus_tag="JQR05_003838"
     CDS             complement(842..1678)
                     /gene="kduI"
                     /locus_tag="JQR05_003838"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY5325836.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(1965..3139)
                     /locus_tag="JQR05_003839"
                     /pseudo
     CDS             complement(1965..3139)
                     /locus_tag="JQR05_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
     gene            3394..4623
                     /locus_tag="JQR05_003840"
     CDS             3394..4623
                     /locus_tag="JQR05_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY5325837.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNHA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1205 a         1191 c         1030 g         1220 t
ORIGIN      
        1 atataaaaaa ccctgccatg cggcagggtc ataaaagtaa gaggaatgaa ttaacgcgcc
       61 agccaaccgc catccacagc aatggtataa ccatttacat aatctgaagc gctggaggca
      121 agaaacacta ccggccccat caggtcactc ggcagtcccc aacggccagc tggaatgcga
      181 tcgagaattt ccgcgctacg ttgttcatct gcccgcagtt gttgggtatt gttggtcgcc
      241 atgtaacccg gagctatcgc attaacatta atgttgtgtt tagcccattc gtttgccatc
      301 aatcgcgtca cacccatcac gccgcttttt gatgcggtat aagaaggcac acggatcccg
      361 ccctggaagg agagcattga cgcgatattg ataatcttgc cgccattgcc ttgcgcgata
      421 aagtgtttcg ccgctgcctg agacatgaag aatacgctct tgatattcag gttcatgaca
      481 tcgtcccagt ctttttcgct gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta
      541 ttcaccagga tatcaatatg accaaattca gctaccgcgc gatccagcag tccaggaatg
      601 ccatcaatct ttcgcagatc ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc
      661 tgctcgatgg tttcagtcgg ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc
      721 gccagcccca acgccatccc ctggcccagc ccagtatcac aaccagtgac gaccgcaact
      781 ttaccttgga gagaaaatgc acttaaaatc ataacaacac cttattcgtt tatgcccaca
      841 actagcgcaa atctttaacg gccacatggt ccatatcatt aaagacctgg ttttcaccga
      901 ccatgcccca gataaaggta taagctttgg ttccgacgcc ggaatggatc gaccagctcg
      961 gggagatcac cgcctgctcg ttatgcatca caatgtgacg cgtttcttgc ggctgcccca
     1021 tcatgtggaa aacgcaggcg tcatcatcca tattgaaata gaaataaact tccatccggc
     1081 gctcgtgggt gtgacacggc atggtgttcc acaagttacc tggagccagc tccgtcagcc
     1141 ccatactcaa ttgacaggtt tccagtacat ccgggacaaa atatttgtta atcgtgcgac
     1201 ggttactggt gaggttatcg cctaacgtga ctggagatac ttcgtccggt gtgacttttt
     1261 tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg
     1321 tatcaatact ggcaaagaca acttcttttg cacctttacc aacatacagg gcgtcgcggt
     1381 gaccgatttc atagcattga ccatcgacag taatcgtacc ggcaccgcca atattgataa
     1441 cacctaactc gcgacgttca aggaaatagc ttacgccgag ttgtttacca acttccccgc
     1501 caacggaaac cgttttagtt atcggcataa tgccgccaac aataattcgg tcaatgtggc
     1561 tgtaaaccat ggtgtactca tcggcgacaa ataccttttc aaccaaaaat tcattgcgca
     1621 gcccttgggt atccagcgtt tttgcgtgcg caccgtggat gctctgtctt acgtccacat
     1681 caacctccga taatcagtga agcgtgaaaa tagaaaacga tagtgaaacg ctgtttcgtt
     1741 ttctctataa agggatgata tgcccatggg ctagggtttt caattaaaaa taaaacaatg
     1801 tttcattttt aagttgagga ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga
     1861 atggaaaaat gagggagccc agaaaatgta tgtcgacttg ccagccatca ttattcaaca
     1921 gattaatgac ggcagatatt tatctgtaag gaggaaatgc agagctactc gtcacgttca
     1981 atggtcaatg ccacgccctg gcccccgccg atacaaagcg ttgccagccc tttgcgggca
     2041 ttacgtttca ccatttcatg aaccagagaa accaggattc ggcaaccgga agcgcctatc
     2101 gggtgaccga gtgcgatcgc gccaccattg acattgaccc gacgctcatc ccactcaagc
     2161 atcttgccaa ccgaaagcgc ctgtgcagca aacgcttcat tagcctcgat aagatcgaca
     2221 tcagccaact gccagcctac acgctccagg caacggcggg tcgcatacac cggcgcaatt
     2281 cccatcaatg cccaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
     2341 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
     2401 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
     2461 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
     2521 tactttggct cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
     2581 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
     2641 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
     2701 catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat
     2761 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
     2821 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagaccg gaaccgcaaa
     2881 cgtcattaat cgtgattgca gaaacgctgt taggaagacc acctttaata gccgattgcc
     2941 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
     3001 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
     3061 cggcagaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga
     3121 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
     3181 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
     3241 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
     3301 atgggtagat agggcgttaa tctcccatac ttaacttggt ttatggtaaa ttgccctcca
     3361 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
     3421 actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct attccttcct
     3481 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
     3541 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcaaa aacatcagca
     3601 ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg taatctgatt
     3661 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
     3721 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
     3781 gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg tcatgccact
     3841 attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt tctttccatt
     3901 taccttgtcg gtagttggca acctgatcta ttaacaaccc aggtagagtt caatcagaat
     3961 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
     4021 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
     4081 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
     4141 tttagctgcc tgctttctat tccatcttcg tatattgaag cggctaaaga agaaggggtc
     4201 accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc aatttccggg
     4261 attattgtcg cagtagttgc gatgtcgaaa tcattcctag gtacgtactt tggcgttatt
     4321 gaaggtgcca cagaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
     4381 gcatttaacc acgcactatc aattatgttg gtatcgctga ttaccttcat tgtttgttgc
     4441 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
     4501 cttttcatca tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc ctggcgttcc
     4561 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
     4621 taatatccgt tgtggatggt tcaaaa
//
LOCUS       DADKXM010000209         4583 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.212, whole genome shotgun sequence.
ACCESSION   DADKXM010000209 DADKXM010000000
VERSION     DADKXM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.212"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            514..1077
                     /locus_tag="JQR05_003841"
     CDS             514..1077
                     /locus_tag="JQR05_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5325838.1"
                     /translation="MKVSKIALAMGLSMALVSGFANAADSGTTPPANTAAEGGHGHGT
                     VHFKGEIIDAPCSISPESADQTVDLGQVANSALKDKGTSSPVAFNIKLQNCDISTYKT
                     VTATWSGTPDVNMPEAWGITGTASGAAIVLRDASEKEITLGQDTAPTTLIADDTTIAM
                     SAFLKGDGEAIVPGAFTGAADFVLSYQ"
     gene            1153..1674
                     /locus_tag="JQR05_003842"
     CDS             1153..1674
                     /locus_tag="JQR05_003842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5325839.1"
                     /translation="MLRIVFLLLLLVSSTGRAVSHGHGHVQMQGTIIDTACAIATGDV
                     EQGIDFGALTASELINNGRGPSLPFTVHLVNCVLDGTDIRGLNHWKDVRITFVGDADS
                     ENHFALQGDGRGAALVIADSQGGEAKPGEPMPSTPITPGSMALHYQLWLTGGKTIRPG
                     HIHTTVRYFMEYD"
     gene            1674..4271
                     /locus_tag="JQR05_003843"
     CDS             1674..4271
                     /locus_tag="JQR05_003843"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025319.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY5325840.1"
                     /translation="MFNQKSGNNGGLNNVRLNSLTWMLLLVLGGIPAAQSQDNMSTPA
                     NSNIPDEVVEQPNITEFNTDVMDVNDRNNIDVSHFSRAGYVMPGEYPLILVVNGTEIR
                     EVTVSFAPPEDDPQGSSPCLTPEMVKLFGLKDEATKKLIWKGNGDDRCLDEKSLPGMT
                     ATGDLSTGSLKISLPQAWLEYSAPDWDPPSRWDNGVADLLFDYNLNASKSHALSEGGS
                     SATSLSGSGTAGANFGPWRLRADWQASRYSDRGYTSQSMDWSRFYLYRALPKLGARLT
                     LGESDLDSSLFDSFRFIGGSLVTDDNMLPPNLQGYAPEVTGVARSNAKVVISQQGRVI
                     YESQVAAGPFRIQELSSSVSGTLDVRVEEQDGRVQHFQVDTASIPYLTRPGLVRYKAS
                     LGKPTDWKHHVQDVSFATGEFSWGMTNGWSLFGGLVAAKKYAALAVGIGRDLLSFGAV
                     SFDVTESKATLDTGEKKMGGSYRLSYSKRFEKIDGQITFAGYRFSEKDFMSMNDFLET
                     EYDAGSRSDTAWGRQKELYTITVSKAFPDWNMNLYGNYSHRTYWNRPDADRYSLSLSK
                     YMDVGRWKGISLSLTGYRSEAWGVKDDGVYASLSIPWSDGGSLSFNGQSTTAGTTQMV
                     GWYDRIDSNNNYSLQAGTSVGNKASASGYVSHNGDLTNGTLSASYYEGNNTSLGLSLQ
                     GGMTVTRYGAALHPGAMNGGTRLMIDSGDVAGVPLSHSGVLQRTNAFGVGVIGNLNSY
                     WRNSVGVDVNALDDDMEVSHPLTELTMTESAIGYRKIDVLEGRKLMAVVKFADGSSPA
                     FGATVWRGSHQTGVVDDNGSVWLAGVKPGVTMEVREDENPVCQVGIPERLPPENTDQS
                     LLLTCQQKS"
BASE COUNT         1192 a          948 c         1221 g         1222 t
ORIGIN      
        1 gacggtgaaa gcaagaagct attaggcgct ataggtcttt tgacggtttt tgaatttccc
       61 ctcctgatat ctctgttata tttttgtgaa tttctccaat ccaaatcaaa gtcccaataa
      121 gtcatggatg catggttaaa ataactatca tttgtgtaac ctttggtgct tataactttt
      181 ctttattctt ttttcaggat tacctggagg gattgaccaa aaaaacattc attgaatgat
      241 atttttctaa ttaatgaggt tttctaagct cgttgaaata aaaaatatat tttaatcatg
      301 ggtttgcatg cgtttctttc gcctttttta cacaagttga catcgaactc agcaactata
      361 agaatagcca catcaccaga cggtgaccga taaaggaaac atatttaaat gaattggaaa
      421 atatcaccga tttgaagtcg actcgttcgg gtaattatta atactgaatc tttaatgctt
      481 atttcttaag cttgttaaca acgggaatga ataatgaaag tgtccaaaat tgcactggca
      541 atgggtctta gcatggcatt ggtaagcggc ttcgcaaacg ctgctgactc tggtacaact
      601 cctcctgcta atactgctgc agaaggcggc catggtcacg gcactgttca ctttaaaggt
      661 gaaatcattg atgcgccatg ctctatttct ccggaatctg cagaccagac ggttgatctt
      721 ggtcaggtcg ctaactctgc cctgaaagac aaaggtacgt cttctccggt cgctttcaac
      781 attaaactgc aaaactgcga catcagcact tacaaaactg taactgcaac ctggagtggt
      841 acacctgacg ttaacatgcc ggaggcatgg ggtatcactg gtacggcgag cggtgcagct
      901 attgttctgc gcgatgcgtc cgaaaaagag atcaccctgg gtcaagacac tgccccaact
      961 acgctgatcg ctgatgacac cactattgct atgtctgcgt tcctgaaagg cgacggcgaa
     1021 gcgattgtcc caggtgcgtt caccggtgcg gcagacttcg ttctttccta ccagtaagta
     1081 gccgcttaac tgtgctggct ccctggttag cagggagccg ttaagaacgt gaaatcagac
     1141 ggaagtatgg tgatgttgcg tattgttttc ctcctgcttt tgctggtgag ttcgacaggg
     1201 cgcgctgttt cacatggtca cgggcatgta caaatgcaag gaaccattat tgatacagca
     1261 tgtgccattg ctacaggaga tgtggagcag gggatagatt tcggcgcact tacggcaagt
     1321 gaattaatta ataatggccg tggtccttct ttgccattta ccgtccattt agtgaactgc
     1381 gttcttgatg ggacagatat tcgaggcctg aatcactgga aagacgtacg tataacattt
     1441 gtaggcgatg ctgatagcga gaaccatttt gctttacagg gagatggacg tggcgcggct
     1501 ctggtcattg ctgatagcca ggggggtgag gcgaaacctg gagagcctat gccttctaca
     1561 cccattacac ctgggagtat ggcattgcat tatcaattat ggctaaccgg cgggaaaaca
     1621 atccggcctg ggcatattca taccaccgtt cgctacttca tggagtacga ctgatgttta
     1681 atcagaaatc cggaaataac ggcggattaa ataacgtcag attgaattcg ttaacgtgga
     1741 tgctgttgct cgtattgggt ggtattcctg ctgcgcagtc acaagataac atgtcaacgc
     1801 cagcgaattc gaatattcca gatgaggtag tcgagcagcc gaatataacc gagttcaaca
     1861 ccgatgtgat ggacgtaaac gatcgaaata atatcgacgt tagccatttt tcccgtgccg
     1921 ggtacgttat gcccggggaa tatccgctca tattagttgt gaatggaact gaaattcgcg
     1981 aggtcacggt ttcatttgca ccgccggaag atgacccaca aggcagctcc ccgtgtttaa
     2041 caccggaaat ggtaaagctt tttggtctta aagatgaagc aacgaaaaaa ctaatctgga
     2101 aaggtaacgg tgatgatcgt tgtctggatg aaaagtcact tcccggcatg accgctaccg
     2161 gtgaccttag taccggttca cttaagattt ctttacccca ggcctggctg gaatatagcg
     2221 cgccggactg ggatccgcca tcccgctggg ataacggagt ggcagactta ttatttgatt
     2281 acaacctgaa cgcatcgaaa agccatgcgt tatctgaagg tggttccagt gcgaccagct
     2341 taagtggtag cggaacggca ggcgcaaatt ttgggccatg gcgtttacgt gccgactggc
     2401 aagcctcgcg ttatagcgac aggggataca cgtcgcagag tatggactgg agtcgatttt
     2461 atctctaccg cgcgcttcct aagcttggcg ccaggttaac tctgggtgaa tctgatcttg
     2521 attcatcact gtttgatagc ttccgcttta tcggcggtag tctggtgact gatgacaata
     2581 tgctgccgcc taatcttcag ggatatgctc ctgaagtgac gggggtagcc aggtcaaatg
     2641 cgaaggtcgt gataagccag caagggcgcg tgatatatga atcgcaggtg gcagctggtc
     2701 cttttcgtat ccaggaatta agcagttccg tcagcggtac cctggatgtc cgggtggagg
     2761 agcaagatgg acgggtacaa cattttcagg ttgatacggc cagtattccc tatctgacgc
     2821 gtccggggtt ggtgagatac aaagcgtcct taggaaagcc tacggactgg aaacaccatg
     2881 tacaggatgt gtcttttgcg acgggagagt tctcttgggg tatgacaaac ggttggtcac
     2941 tatttggtgg cctggtcgca gccaaaaaat atgcggctct tgcagtgggt attggtcgcg
     3001 atttgctgtc atttggtgcg gtttcgtttg atgtcaccga atctaaggcg acgcttgata
     3061 ctggcgaaaa aaaaatgggt ggttcatacc gcctgagtta ttccaaacgc tttgagaaaa
     3121 tagatggaca gataacgttc gcgggctatc gcttttcaga aaaagacttt atgtcaatga
     3181 acgattttct cgaaacagag tatgacgccg gtagcaggag tgatactgcc tggggaaggc
     3241 agaaagagct ttacaccatc acagtcagta aagcttttcc tgactggaat atgaatctgt
     3301 atggcaatta tagccaccgg acctactgga atcgtcccga tgcagatcgc tatagcctgt
     3361 ctttatcgaa atacatggac gttgggcgtt ggaaggggat tagcctgagc ctgactggct
     3421 atcgcagtga ggcatggggt gtgaaggatg atggtgtata tgccagtctg agcattccct
     3481 ggagtgatgg cggttcattg agtttcaacg ggcaaagcac aacggctggc acaacacaga
     3541 tggttggttg gtacgacaga attgatagta acaataatta cagtctgcaa gccggtacaa
     3601 gtgttgggaa taaggcctcg gcgagtggat atgtgtcaca caatggtgat ctcacgaatg
     3661 gaacgctgag tgcgagttat tacgaaggaa acaatacgtc gttagggctg tctttgcagg
     3721 gcggaatgac agtaactcgt tacggagcgg cgttgcatcc tggtgcgatg aacggcggta
     3781 cgcgactgat gattgattct ggtgatgttg ctggtgtgcc gttaagccat agtggggttc
     3841 tgcaacgcac gaatgccttt ggcgtcggcg tcattggtaa ccttaatagc tactggcgca
     3901 acagtgttgg ggtcgatgtt aatgccctgg acgatgatat ggaagtcagc catccgttaa
     3961 cggaattgac catgactgaa agtgcgatcg gttatcgaaa aatcgatgtt cttgaaggtc
     4021 gaaaattgat ggcggtagtg aagtttgcgg atggtagtag tccggcattt ggcgccaccg
     4081 tctggagagg gagccatcag accggcgtcg ttgatgataa cggtagcgtg tggttagccg
     4141 gggttaagcc gggtgtcacg atggaagtcc gtgaagatga aaatccggta tgtcaggtag
     4201 gtattcctga gcgattaccg ccggaaaata ccgatcaatc gctgttatta acatgccagc
     4261 agaaatcata actcattatg tgagtggggc actgtattgg aaaaacagtg tgtgtagaga
     4321 ttaggagaaa aggacgcggc catggacggt acattaatgt tgataattaa attcagagaa
     4381 tcaaatgaac agaaataaat caattaagtc ccagccgttc ctgaaatctc ttctggtcat
     4441 ggctttagtc agtcagtccg catttcaggt tgctgatgcg gcagttgctc tggatcgtac
     4501 tcgggtaatt tttgaaggcg gtaataagag catgagctta aatattcgta acgataatac
     4561 gaagctccct tatttagcgc aat
//
LOCUS       DADKXM010000210         4500 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.213, whole genome shotgun sequence.
ACCESSION   DADKXM010000210 DADKXM010000000
VERSION     DADKXM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.213"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(90..754)
                     /locus_tag="JQR05_003844"
                     /pseudo
     CDS             complement(90..754)
                     /locus_tag="JQR05_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(952..1989)
                     /gene="curA"
                     /locus_tag="JQR05_003845"
     CDS             complement(952..1989)
                     /gene="curA"
                     /locus_tag="JQR05_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY5325841.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLAPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGEMLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMVQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            2170..2688
                     /gene="mddA"
                     /locus_tag="JQR05_003846"
     CDS             2170..2688
                     /gene="mddA"
                     /locus_tag="JQR05_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY5325842.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPMLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2685..3134
                     /locus_tag="JQR05_003847"
     CDS             2685..3134
                     /locus_tag="JQR05_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5325843.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(3135..3368)
                     /locus_tag="JQR05_003848"
     CDS             complement(3135..3368)
                     /locus_tag="JQR05_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY5325844.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(3454..3627)
                     /locus_tag="JQR05_003849"
     CDS             complement(3454..3627)
                     /locus_tag="JQR05_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY5325845.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFTVALLFSLF"
     gene            3822..3917
                     /gene="yncL"
                     /locus_tag="JQR05_003850"
     CDS             3822..3917
                     /gene="yncL"
                     /locus_tag="JQR05_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY5325846.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(4319..>4500)
                     /locus_tag="JQR05_003851"
     CDS             complement(4319..>4500)
                     /locus_tag="JQR05_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HAY5325847.1"
                     /translation="IDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQE
                     RDYKKFNLSEVFRGL"
BASE COUNT         1117 a         1097 c         1004 g         1282 t
ORIGIN      
        1 tgcgcccgga cgaaattatg gtgtcggcat gaatattgcg tggcgatttg agtaattact
       61 tgatgcccgg tatttgccgg gcatttaatt taacgattgt attgctggta taaaatagca
      121 atatttgttg cataatttca gcaagacaat gtttgcatcc ctctttgtct tttgctttta
      181 acgcagcgag taatagatga tagttttcta tgcgctcccg taattacgct gggttaatcg
      241 cttcataaag ataatgtaaa gcaggtccca tcctgatcca cagttgctca atcatctcac
      301 acaggatggg catatttgag cgatgataaa tcgctaagcg aaatagcctg ttcgcattaa
      361 tgatttgttc catgtcgccc ttttcttgcg cctgctgtaa tttcccgagc aattcctgaa
      421 gttctgctat gtcttgcgag gagaggtttt caacagccag ggcaactgcc attaattcca
      481 gttcgtagcg gatccgattg atttcgtcta actgactttg cctcacttcc ggtactgtaa
      541 atgcttgtgc aggtgcgaca gaaagcgcat tcaccgaaac cagacgtaat aatgcttcac
      601 gcacaggtgt aatactcata cctaattgct ccgccagatt tttagtaatc aggcgtgcgc
      661 caggttttaa tgcgccaata ctaagctgat gtttcaggtc gttctcaacc tgcagagtca
      721 aactgacatg tttcattttt cccgttccag gcattttaat tccttcgcgt gttttctgtc
      781 caaccctgcc tgcatggcag aaggagttca cctggtgtat taaagtgaat aatcataaac
      841 cacccacctt tgttgttatt agcagcagaa caatgacgtg gttagttaat taaagctgaa
      901 aataagcttt ttgcctggat ttttatggcg gcttacgccg ccgttgaaac tttaatcatc
      961 acccgcgacg cggatcacca ctttgccgaa gtttttaccc ttcagcaggc cgataaacgt
     1021 ctgtggcgca ttctctaaac catcagtaat atcttcgcgg tagtggattt tatcctcttt
     1081 cacccattgc accatctcct tctgaaactc atggatgcgg tgaccataat cctgagcgat
     1141 aataaaccct tgcaagcgaa tacgtttttt cagcaccgtt gccatcaaca ggggtaaacg
     1201 atccggaccg ggtggtagct ctgtagcgtt atagctgctc actaagccac aaacgggaat
     1261 gcgcgcagat gtattaagta atggtagcac tgcatcaaat accttaccgc ccacgttttc
     1321 ataatagata tcaatacctt ttgggcacgc tttcgccagt tgttcggcaa aatcgtccgc
     1381 gtggtgatca aggcaaacat cgaagcctaa cacctcggta gcatggcggc atttttccgc
     1441 gccaccggct acccccacca ctctgcaacc tttaagtttg ccgatttgcc ccaccgtcgc
     1501 ccccactggt cctgtcgccg cagctaccac caacatttcg ccctctttag gctgaccgat
     1561 atccagcagc cccatataag cggtaaagcc tggcatcccc agcacaccca gcgaccacga
     1621 tggattttgc ggatgatcgc caagtttcac caggtcatca ccactggata tgtcatagtc
     1681 ctgccatcca ctgtagccca gcacccagtc gccaggctga taatcaggat gattcgactc
     1741 cacgacacgg ctcaccgtac cgccgaccat cacgccgcca atatcaaccg gcggtgaata
     1801 agatggctca tcgctcatac gtccacgcat atacggggcc agggacaaat aaactgtgcg
     1861 cagtaacacc tgtccttcac ccggtgtggc gacatcatct tcttcaagac ggaaattctc
     1921 cggaaccggt gcgccatgtg gacgcgaggc cagaacccaa cgtcgattac gctgcttttg
     1981 ttgccccata atgctctcct gtgcttttgg catgaatcca tcaatagtag caggctcacg
     2041 ggattggttt tgtttgcagc cgtcacatcc cctctgcaac acgcaataat ttcatcgagg
     2101 aatccgttgc ttcgggctgt tttcgatagt attgataaca tcatcatcat tattttattg
     2161 aggccagaca tgtccatccg ttttgcccgc aaagccgact gtgctgccat tgcggaaatt
     2221 tataaccacg ccgtgttgta tacggcggct atctggaatg accaaacggt ggatgcagat
     2281 aaccgcattg cctggtttga agcgcggacc atagcaggtt atccgatgct ggtaagtgaa
     2341 gaagatggcg tagttacggg atatgcctcg tttggcgact ggcgtagttt cgatggtttt
     2401 cgccataccg tggaacattc ggtttatgtc catcccgatc atcagggcaa aggtctgggg
     2461 cgtaaattgt taagccgatt gattgatgaa gcgcgggatt gcgggaagca tgtcatggtc
     2521 gccgggatcg aatcgcaaaa tcaggcttcg ctgcatctgc accagtcgct gggatttgtc
     2581 gtcaccgcac aaatgccgca ggtaggcact aaatttggtc gttggctgga tctgacattt
     2641 atgcagttgc aactcgacga gcgcactgaa ccggacgcga ttggatgaat cagtcgctca
     2701 cccttgcttt tttgattgcc gccggaattg gtctggtagt ccagaacacg ctgatggtgc
     2761 gcatcaccca gacctcctct accattctca tcgccatgtt gctgaactca ctggtgggga
     2821 ttgtgctctt tgtttctatt ttatggttta aacaaggcat ggcggggttt ggcgaactgg
     2881 tgtccagcgt gcgctggtgg acacttatac ctggcttgct gggttcgttt tttgtcttcg
     2941 ccagtatcag cggatatcag aatgtgggag cggcgacaac tattgcggta ctggtcgcca
     3001 gccagctgat tggcggtctg gtgctggata tcttccgcag tcatggcgta ccactacggg
     3061 cgttgttcgg cccaatatgt ggcgcaattt tacttgtggt tggcgcctgg ttggtggcca
     3121 gacgctcatt ttaatcagag gatggtgccg cctttggtta actgttcgtg gcgcgcttcc
     3181 atatcttctt tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg
     3241 gtgtagcgat cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc
     3301 atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga
     3361 tgcgacatac tttctcctta gccacttgtg aaatttatcg attaatccaa atgatataag
     3421 tctgcgtggt gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac
     3481 cgtgaaactc atcggccatg tggcccccac cagaaaggcg cttaagaaac ggatgcgttt
     3541 cttatcgtga gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa
     3601 aacaagcata tgttgataga gagacatgta cgggcgtcca gccatatgaa taaaacgcgc
     3661 gcaaatatgc gtcatttttt gatgtgcatc aagttttttc accagtttga gaaaaaatca
     3721 tcaaacagca acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac
     3781 ccgtactata cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa
     3841 ctgtagtact gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat
     3901 ttggctggtt tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac
     3961 tctctgacag tacgcaagat cttctcatac tctccaggtt tgcaacatta tccttaatat
     4021 ttacttcttt ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc
     4081 tatacaaaca gcttactgat tcaaacggaa tcttgttttc acatttttct atcacgaata
     4141 ctttatacgc gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa
     4201 aagatggcgt aaagtatatg catggcagga tgtttgcaac ctccttgatg acgctgcccc
     4261 attgaaattg caaaccccgc cctggttatc ttgcccattt atcatgcaaa acagataatt
     4321 ataggcctct gaatacctca gaaagattga attttttata gtccctctct tgaatatttc
     4381 catttgatat caatcgattc atctcttcaa caaaataatt tatatcatca cacttgttat
     4441 tatccggaag atctgagtag ttataattcc accattttat tctcaacagt tcatcaataa
//
LOCUS       DADKXM010000211         4447 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.214, whole genome shotgun sequence.
ACCESSION   DADKXM010000211 DADKXM010000000
VERSION     DADKXM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.214"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            143..391
                     /locus_tag="JQR05_003852"
     CDS             143..391
                     /locus_tag="JQR05_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325848.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            724..2247
                     /locus_tag="JQR05_003853"
     CDS             724..2247
                     /locus_tag="JQR05_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5325849.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPLLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2343..2576
                     /locus_tag="JQR05_003854"
                     /pseudo
     CDS             2343..2576
                     /locus_tag="JQR05_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2998..3207
                     /locus_tag="JQR05_003855"
     CDS             2998..3207
                     /locus_tag="JQR05_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325850.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            3324..>4447
                     /locus_tag="JQR05_003856"
     CDS             3324..>4447
                     /locus_tag="JQR05_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5325851.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYT"
BASE COUNT         1264 a          879 c          976 g         1328 t
ORIGIN      
        1 gtgttgcgat acacagctga tgatatgtaa cttctaatta ttaagtataa gtttatagaa
       61 aactcattca tcttattttt gtctgtcgct ttagactata cacagcataa ttttattggg
      121 ttaatatttc tacgaggctg acatgaataa ttcaatccca gagagattta tttttcaatg
      181 tgctttgttt aaaaatctcg agagggaggt gttcatgacg catgggtatg ttgatagtca
      241 tattattgat caggctttac gcttgcgttt aaaagatgaa accagtgtga ttctttctga
      301 tctctatctg caaatattgc agtacattga aatgcataag actacactaa cggatatcat
      361 tattaatgac agggaatccg tgctctctta gaacttgagt aaggttttag aataaacatc
      421 agctgtatca ccatgctgat gcaaagtgag catttcaggc gttatgcttc cttattatgt
      481 ccgcaatatc aggtgtcaag aatggagagt tctcgctctc cattcttgac acctgatatc
      541 ccgcctaact tattttgtac tgcctacatt atgctcaata attaatgggt tagatcaaga
      601 tggagaaaat tttatcttcg gcgtggatat ttcttatgct ctctaccatg catctctgta
      661 gatgccagcg cgagcgaatg aatggcattt tcatgtatta aaccctttgg ccgggacaag
      721 caaatgcgca atacactcat acccatcctc gttgcgatat gcttgtttat taccggtgtc
      781 gctattttga acatccagct ctggtattcc gcaaaagcag agtacctggc gggagcaaga
      841 tatgccgcca acaatatcaa tcatatactt gaagaagcgt cacaggcgac tcaaacagcg
      901 gttaacattg ccgggaagga atgcgacctc gaggagcaat atcagcttgg cactgaagca
      961 gctctgaaac ctcacctgcg cacaatcatc attctcaaac agggaatagt ctggtgtaca
     1021 tccctgcctg ggaatcgggt cctgctgtct cgtattcctg ttgtcccgga cagtaattta
     1081 ctgttggctc cggcaatcga caccgttaat agattaccta tcctgctcta tcagagccaa
     1141 tttgcagata cgcgcatttt ggttacgata agtgatcaac atattcgcgg ggcgcttaat
     1201 gtacccttga aaggggtaag gtatgtatta cgcgtggcgg atgacattat tggaccaacg
     1261 ggtgatgtga tgacgcttaa tggacattat ccctataccg agaaggttca ctccacaaaa
     1321 tatcatttca ctattatctt taacccgccg ctactcttta gcttctacag acttatcgat
     1381 aaaggctttg ggattttaat atttattctg ttaatcgcct gcgccgctgc cttcctgctt
     1441 gatagatatt tcaataaaag cgcaacgcct gaagagatcc tgcgacgggc aataaataat
     1501 ggggagatcg tcccttttta ccaacctgtg gtaaatggtc gggaagggac attgcgggga
     1561 gttgaggtgt tagcccgctg gaaacaacct cacggtggat atatttcacc cgcggcattt
     1621 attccacttg ctgaaaaatc cggattaatc gttccgctta cacaaagcct gatgaatcag
     1681 gttgccagac agatgaacgc tatcgcgagt aaactgccgg aaggttttca tattggaatt
     1741 aattttagcg cttcgcatat tatttcgccg acgtttgtcg acgagtgttt aaatttccgt
     1801 gacagtttta cccgccgcga tttaaacctt gttctggaag tcaccgagcg tgagccattg
     1861 aatgttgatg aaagtctggt tcagcggttg aacattctgc atgaaaatgg ttttgtcatc
     1921 gcgctggatg atttcggtac tggctactca gggctttctt atcttcatga cctgcatatt
     1981 gattatatca aaattgatca cagtttcgtt ggccgcgtaa acgcagaccc agaatcaacc
     2041 cgaattctgg attgtgtatt ggatctggcg cgtaaacttt cgatcagtat cgtcgctgaa
     2101 ggtgtcgaaa cgaaagaaca acttggctat ctgaaccaaa ataatatcac atttcagcag
     2161 ggttattatt tctataaacc tgttacatac atcgacctgg tcaaaattat cctttctaaa
     2221 ccgaaggtga aggttgtggt tgagtgaaaa ttgatcagta aggccatagt gcggtgtaat
     2281 tatagacagc taattagctc gttgcctctt gttactattg ttcattattt tgtttgctat
     2341 aattgtttga aagttttgac aggattgcca ttagtagcat gaacaatagt aataatctgg
     2401 attatttcac tctctatatc atcttttcca ttgcatttat gctgatcacc ctcctggtca
     2461 tccttattgc aaaacccagt accgggctgg gagaagtgct tgtgacgata aatttgctta
     2521 atgcccttgt ttggctggcg atcaatctgg ttaatcgatt taagagaaag actcgtcagc
     2581 cacagggatc agcaataatc tttcagtttc tcactgtcag tatgcggctg aatgggttgc
     2641 tggcagtgaa cgcctggatc attgaaggaa aggaattatt gcgcaaatag ttgtcaacct
     2701 tggtgttatc acagttgttt ttatatatca ccgaaataat taccatggca actattaata
     2761 attttgatgt cgaagagtta tttgttaaac aaaatagtca ccccaaagtg atcaatgtca
     2821 tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat tgtcgctgtt
     2881 tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt ggtaatctga
     2941 gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg ttaatctatg
     3001 aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt gggtaatgca
     3061 tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat aaaagatgga
     3121 aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag ttacgggtat
     3181 ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta acaacgggcg
     3241 gtttatatcc ttctgacaat agtcctctaa gcccagcatt aacagctagc gcaatcgtaa
     3301 gtgaatttgg cggtgctgtt attttgcagg gtaaaaacac gattgtcaca acgggggatt
     3361 actcaattgg tttgctaagt caaacgagtg gaaacctgaa tactgacacc ataataagag
     3421 tcaactctga cggttcggtt accccctctt tttctgacgg agatgataca tttattgtta
     3481 ctgcgggtaa tcacgcagtc ggcgttcttg cgtgtgcatc tcccggaagc gcgcgtgcgt
     3541 gtgtatcttc tcttgatgaa gaaagtactg ccgatacagg aagtaatgaa aataatgcca
     3601 tagcaaaact ggatatggca aaaggggaga tcacaaccca cggaacagaa agttatgccg
     3661 cgtacgctaa tggcactgtc gtcaaggctg gtgacacgct tgattatacc aatgccagcg
     3721 ttacattaac cgatgttgat atcaccacgc atggggacaa tgctcatgcc atcgctgccc
     3781 gccaggggac agtttcattt aaccagggag aaatttatac aacgggtcct gacgccgcga
     3841 ttgctaaaat ttataatggc ggcacggtga cgctgaaaaa tacatctgca gtcgcgcatc
     3901 aggggtctgg aattgtgctg gagtcttcca taaatggtca ggaagcaacg gtagatattt
     3961 tatctggtag ctcactacgg tcagcaaatg aaatcctcta ccataaaaat gagacgagta
     4021 acgtgaccat tacggatagt gaggtatcat cggctgcaga tgtttttatc aataatatta
     4081 aaggtcattt gaccgtcgat gcaactaatt caaaaataac gggttcagct aatatttcaa
     4141 cagatgataa cactcatacc tatctgtcgc tttcagataa tagtacctgg gatattaaag
     4201 ctgactcaac ggtgagcaac ctcacggttg ataatagtac ggtctatatt tcccgggcag
     4261 atggacggga tgtcgaacca acaagattaa caataactga aaattacgtt ggtaataatg
     4321 gcgtattgca tctcagaact gaattggatg atgataattc agctacggat aaagtcgtta
     4381 ttaatggaaa tacctctgga acaacccgag ttaaagttac caatgcagga ggcagcggag
     4441 cttacac
//
LOCUS       DADKXM010000212         4377 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.215, whole genome shotgun sequence.
ACCESSION   DADKXM010000212 DADKXM010000000
VERSION     DADKXM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.215"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1233)
                     /locus_tag="JQR05_003857"
     CDS             complement(<1..1233)
                     /locus_tag="JQR05_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5325852.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHG"
     gene            1450..2373
                     /locus_tag="JQR05_003858"
     CDS             1450..2373
                     /locus_tag="JQR05_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5325853.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2411..4051)
                     /gene="trg"
                     /locus_tag="JQR05_003859"
     CDS             complement(2411..4051)
                     /gene="trg"
                     /locus_tag="JQR05_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY5325854.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SKQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRNRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         1050 a         1153 c         1035 g         1139 t
ORIGIN      
        1 accgtgactg cgtgtctgcg tttcattacc cagatttgac tggaagatcc ccgccgcgct
       61 aacgggcaag aaatcttcat aggtgatggg ttgcgccact acccaaccac gttcaattaa
      121 gggctgtgga tcgtctccag gatgaatcgc ctgacgatgc gcctcacccg aaggcgtcag
      181 acggtaccgg aaccatgcca acccttgctg acgcattaaa aactcactgt caggaaaagt
      241 gcggaaggtt tcctgtaaat gcatttggtg agtgagatta tcctgcccgg ttccagcgtt
      301 acgcagaaga tcatcataca gttgtcgccc tttcggcgtt aatgccacgc cacgctgctc
      361 aatttcacca aagcgcgcgg tatgcgtgcc ctgtttctgc cccgcaaaca acaccgtctc
      421 ttccagtgct ttaaagctgg tctggcgtag taaaatcggt acctcgcggc gcggcggccc
      481 ctcgatgaga attttgggtt caattccgca ttcaggcatc atcgactgca cccggtcaat
      541 atccagcgta cgtggcgtca ggtggttgat atggcatcca ggaaaacaga ccacatcagc
      601 aatcaaccga tgttcgttgt gcaaggcgcg ataggtttct tcatctaccg ttgctaactg
      661 gtgccagcga aacgtttcca gggcttcctg cacaaactcc tgtgcctgtg tttcgttaaa
      721 accgccctgc tgctcatatt cctctaacag ttgtcgacaa cgtggggtga agatatcgcg
      781 ctgacgcaga atctccgccg ctttctggcg caaaatttcg ttctcgataa gctcaaggcg
      841 gagtaaggat gtaaaaacgc ggaagggatt acgcgccaga gaagcatcat caatgggccg
      901 aaatgctgtc gaatgtaccg gcacccctgc ctgcgagaga tcgtaatagc tcaccgggta
      961 catccccata atggcaaaca tccgccgaag agtagcaagc tcttgtgcag tcccaacgcg
     1021 aatcgcccca tgacgttcaa cattcagtcg cgccagctcg tctgcattta ccattttttc
     1081 gtgcagttga ggattgtttt ccagcacagc cagattcaca tcagctacca gttccagcag
     1141 cgtgccatat tgcggaactt cttgctggta catggctgac attgcctgcg aaaactgttc
     1201 ccgaatctca tccgccgtga tgctgttcgc catagtgtct gcctccagtg gtcagtaatc
     1261 tggagtgtag gtaaccgcat tcactctggc gggaagaatt tacaaactgt gatctcgccg
     1321 cgaaaacatc aatattatcc attttgctgt aacataattg ctttaattgt taataatatt
     1381 ttgcaatcaa gttatcataa tcaaacaact tcacttgtca gcgacaccgc ttcgttttta
     1441 acatcgctta tggaaaaaaa tagtctgttt agtcagcgca tccgtttgcg ccaccttcat
     1501 acattcgtag ctgtcgcaca acaaggaact ttggggcgcg cggctgaaac ccttaatttg
     1561 agtcaacctg cgctctctaa tacattgaat gaactggagc agctgacggg cgctcgcttg
     1621 tttgagcgtg gtcgtcaggg ggcgcaactt accttacccg gcgaacaatt tttaacgcat
     1681 gcagtcagag ttcttgacgc catcaacact gccggacagt cgcttcatcg taaagaaggt
     1741 cttaataatg atgtcgtcag ggttggtgca ctacctactg cggcactggg gatattacct
     1801 tcggttatag gtcagtttca tcagcaacaa aaagagacga ccttgcaagt tgcgacaatg
     1861 agtaacccta tgattctggc gggtttaaaa accggggaaa tcgatatcgg cattggtcgg
     1921 atgtcagatc ctgaactgat gaccgggctt aattacgaac tgctgtttct tgaatcgttg
     1981 aagctggttg tccgccctaa tcacccgcta cttcaggaga acgtaacgct aagccgggtg
     2041 ctggaatggc cggtcgttgt atcaccagaa ggcactgcgc cacgccagca ttcagatgca
     2101 ttagtgcaga gccagggctg taaaattcct tcgggttgta tcgaaacgct gtctgcttcg
     2161 ctatctcgtc aacttaccgt tgaatacgat tacgtgtggt ttgtcccttc tggcgctgta
     2221 aaagacgacc tgcgtcatgc cacgctggtg gccctgcctg ttccgggaca tggtgcaggc
     2281 gaaccgattg gaatactgac ccgcgtagat gcgacgttct cttctggttg ccagttgatg
     2341 attaacgcta ttcgaaaatc aatgccgttc tgaaaggtga agggatctgt cgatccctcc
     2401 ttgaacattt tcacaccgta gcgaaactaa ctggttcacc cgctccgcga ggttctgccg
     2461 acacagaatg tttgttcaga cggaatacgt tcaccgcctc agttaatcgt gcagcctgtt
     2521 cttcaaggga caccgccgct gctgaggcct cttctaccag agaagcattc tgttgcgtca
     2581 ccttatccat ttcagaaatc gcctggctaa cctgagttat gcctctactt tgttcatccg
     2641 aggcggcggc gatttcctgc atgatatgtg tgacactcgc gacggcatca acaatagtgc
     2701 tcatggtttt ccctgccgtt gccacctcat ccgaccccag gtcaattaac ctgactgatt
     2761 cactgatcaa gccttcaatc tctttcgctg cctgagcgct gcggcttgcg agtgtccgta
     2821 cttcgctggc gacaacggca aatccacgcc cttgctcacc cgctcgcgcg gcttcaacgg
     2881 cagcattcag tgccagaata ttcgtctgga aagcaatact gttgatgacg gcggtgatct
     2941 cagaaatttt cttcgaactc gtggagatag cgcccatcgt ttttactaca ccggaaaccg
     3001 tctgcccgcc atcgctggct ttaatagaag cctcttgcgc cagtttgctg gcatgatgcg
     3061 cgttatccgc attctgtttc accgtcgcag tgagttgctc catgctggcg gcagtttgtt
     3121 cgatagccgc cgcttgttct tcggtgcgag atgacagatc cgcattgcca gctgaaattt
     3181 cgctggtgcc acgataaatc tcttccgcgc cctgtcgaac agtccctact gtcatcccca
     3241 gtgaatgctg catttgctgt aaatgacgac ttaagcgacc gatttcatta cgacccgccg
     3301 gttcatcatt catcgtcaga tcgccactgg cgattttttc aatccgttgt gcggcatgtt
     3361 gcagtggacg aatgacgatc cgacgtagca ccataaatgt tatcagcgtc atgaccaggg
     3421 caagcacaaa cgcgccaatc atgaacatcc cacccaggcg gttgcgctga tgggccagtt
     3481 ccgccagttg gttggctctg gtgctacgta tcttaacggc tttgttcaaa atatcggtat
     3541 aagcattatc cagcggtcgg atctgcttac tttcatgatt gataatcgct tcaaacatgc
     3601 catttttggc atatttcaac ataggttgca tacccgtgat ataagcctga aagcgttgat
     3661 ttaattcagt gtcgagggct tcatcagcag gtgttttcac cggtcgattc tgataagcac
     3721 gataaccttg ctgcgactgt ttaatctccg attcggcttg cgcaatattt cgcttcattg
     3781 cttccatttc cgcaatacga ctcgcagccc cggcctgaat catattgatc cgggctgaac
     3841 gcaaaaaatc tgaactgttc gctaacccgg tccgaatctc aatctccgca gtaacatcac
     3901 gctgatcgcg atcggcctgc cagaggaaat agccagccag agcagaactt aatgcgaata
     3961 gaactaagat accgccaaga atgcaggcaa ataacggaac cagcctgatg tgatgcaaaa
     4021 aacctaatcg ctgtgaggga gttgtattca ttaccgttgt ctctcgtcca ggtttactcc
     4081 tgatagaaag tcatcggcac ttgacggtaa ttacttatga ccaaaaacct ctttgcgtca
     4141 catttttaca acaattattg cagagaaaat taatgctatt ggaatgaaga gagtaacgaa
     4201 tggctaaact ggctaacccg aatgacgaaa aattctggaa aacagcacgc aatttcgtga
     4261 agtgattatt ttcgtcccta aggaaacctc ataggatgcc tgcgggtgcc tgagactttc
     4321 tgttttcagg ctaaccggga atcagaaaga cgaaggcccc gaccgatatt tctatca
//
LOCUS       DADKXM010000213         4371 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.216, whole genome shotgun sequence.
ACCESSION   DADKXM010000213 DADKXM010000000
VERSION     DADKXM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.216"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..3319)
                     /locus_tag="JQR05_003860"
                     /pseudo
     CDS             complement(1..3319)
                     /locus_tag="JQR05_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(3363..3944)
                     /locus_tag="JQR05_003861"
     CDS             complement(3363..3944)
                     /locus_tag="JQR05_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY5325855.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKNLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
BASE COUNT         1034 a         1031 c         1007 g         1299 t
ORIGIN      
        1 ttcgccgtca gtggtgtcat aaccaatcac cacgccattt tccgttacgc tagtgtcacg
       61 agaacctttg gcaatcacac ggctggaaac ggagctgcta cccagcgcaa taccggtatc
      121 aacggtgctg ctgctgttct gaccaatggc tacgctgctg gcaccagacg cgacagcgtt
      181 ataacctaca gctgtagcgc cgacaccaga ggcgcttgca tcgttaaagg ctaaatcgtt
      241 gtcgttagta cgaacgtagt tgataccagc gccattttct tcgatatagg ttgcgtcggt
      301 gttaccagcg agctgattga taatttgggt gttctgctca atcatcatat tcgtcgcatt
      361 caactgagaa ccgttcactg catcggtact gtcctcacta atactgccat tagcgacatt
      421 agtgatgatg ctggctttac cgtcatggct ggcattgtag gctccagcac cgccattggc
      481 agtctcatcc cacagcaggg cgttatcatc aagcgcatcc agggcgtcac cgacgttgtt
      541 gtactcggcg ttcgcaatgg tgtaagtcgg tgcggtgatg gtgccgtcag cgttgacttc
      601 tgcgtcacca cccagcgcat cagcgatgta cttattggtg gtatagagtt gtgagccgtt
      661 aatcgcgtcg ctgctggcag cagagattgc accgttagcg acgttagtga ttacactggc
      721 agttttatct tttccgtgag cggcgctaaa tgcaccattt tcaccggcgt ccgcatccca
      781 gagcagagcg tcgtcaggag tggtatcgat agcattcagg gcatcaccga cattatcgta
      841 atcagcatta gcaatggtgt acgtcggcgc agtgatggtg ccgtctgcat tgacttcggc
      901 accacccccc cagcgcatca acaacataac tgctcacgcc gtggagttgt gagccgttta
      961 ctgcgtcaga actggagtct gaaatttcac catctgcgac atcagtgatc acgcttgcgt
     1021 caccattagt accgtgtttg gcactgaatt tacctgcggt ggcatcccaa agcagagcat
     1081 cgccgagaga cgtgctaaat gaggagttaa tcgcagccag tgcatcgccg acgttataat
     1141 aatttgtttc accgattttg taggttggac cggtaaacac accgtcagta ttcacagaag
     1201 cattgccgcc gaagtaggtg gcgatattgc tgcttaagtc ataaagctgg ctaccgttaa
     1261 tggcatcgga actggttgca gtgactgtgc cgtccaggat attggtgatt ttgctggcgg
     1321 tattgttgcc atgagctgca gtgaagacac cattgtcctt atcccatttc agcgcatcct
     1381 caccaagatt agtcaccgtc tcagtcagat tagagatgtt ggtggtgtta gtggcgatat
     1441 tggtggtatt cgtcgccacg gcatcgttgg tggttttcag ctgagagccg ttaacggcat
     1501 cggtgctgcc agtcgtcagg tcgccatctt taacgttggt tattttgctg gtggtctcgg
     1561 tgccatgagc tgccgtgaat acaccattgt ccttatccca tttcagcgca tcctcaccaa
     1621 gattagtcac cgtctcagtc aggttagaga tgttggtggt gttagtggca atattggaag
     1681 tgttattggc gatattggtg gtattcgtcg ccacagcatc gttggtggtt ttcagctgag
     1741 agccgttaac agcatcagtg ctgtcagccg tcaggtcggc atctttaacg ttggtgattt
     1801 tgctggtggt atcctggccg tgagctgcac tgaatgcctt tttagtttcg ttccagagca
     1861 gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg gtggtgttgg
     1921 cttcgacatc gtcattggta gctttcagtt gggaaccgtt aaccgcatcc ttactggagg
     1981 ctgaaatcgt gccatccgca acatcggtga ttacgctggc agttgggcta ctagtaccat
     2041 gagcagcgct gtatttgcct ttggtttggt cccattgcag ggtgttttca tcaagtacag
     2101 cgagcgcagc ccctacgtta tttttgctac cattttttaa attgtaggtt ggtgctgtaa
     2161 cagtaccgtc atcaacatct actgcagccc ctcctccaag cctttttgct accgagtcgc
     2221 taatggcata aagctgtgaa ccattaatgg catcgtaact gtcagacttg atcgcgccat
     2281 ttttcacatt aacgatcttg cgttttaatg aactattgcc gacagagacg gtattgtcta
     2341 aatccgcaat ggaatctgcg cccagcgcga cagagttaat accgttggca ctgctattac
     2401 taccaagcgc aagactctta tcaccagttg cattactggc tgtgcctaag gcaatggtgt
     2461 ttaagcctct cgactggcta ccattaccca gagcgatggc atccacgcca tcagctttgt
     2521 tgccctggcc tatggcgatt gcattatctg ccagagcctg gctgccattt cctaatgcga
     2581 tagtattgac tttatcggca aggcttccga accccattgc catgctatta gtcccttttg
     2641 ccttcgcatt attgccaata gcagttgcat tttcagcggc ttcggtctca gcacctatcg
     2701 caatagcgtt cgctgcgctg gcaagtgatt gtctgccaaa tgcgaggctg tcagtaccgc
     2761 tagctacact gctacgcccc agggcaatgg cgtttgctcc ttccgctttg gaagagtccc
     2821 ccatagcaat agacaattta cccaatgctt ttgaataacg gccgagggcc attgagtatt
     2881 ggcccaatga atacgaagag gcacccagtg ctatacttct atcgccttct gatattgcac
     2941 taaccccaaa ggccattgat gcaccaccaa tcgcatgggt ttttgacccg atggcagagc
     3001 tatattggcc ttcagctata gcgtcataac ccacagcagt actggaacca ctggtgttca
     3061 taaaagtatt tgctttcgcc cctttgccta taacaaccca gccgtcgcca gctgatccac
     3121 taccgtaatc aacaccctga ccgttgtcat tcccggcatt cgccaatgcc ccaaacgacg
     3181 acaacattcc acccgcaacc agcgcagaaa ttaacagctt actgcgccca gatttcttgc
     3241 cacggctttt tgccgtttcg ctggtaacag tataattccc tgtcgcaggg ttccagataa
     3301 ctttaaatat tttgttcatt aatgatgctc gctattcctt gttataagcg tactaactgc
     3361 atttattttt tgcttctttt ctgccgattt tcagcagagg gaagattgtc gaactgaata
     3421 ttttccatgt tgatggctaa cttcagatat cccaggttat tgggcgatac acccttcagg
     3481 gtcaaattaa tgtcttgcca ttgacctggt aataatttca atgtggattt cattgatgtg
     3541 ttgaaaagta attgttgcca ccgggcaaac tctttggcat cgacaggttt ttcaccaccc
     3601 catgtcgcat taaaaccaat gtttgagata ggtgtactta ataagttgac cagtgagata
     3661 tgaataacac taccattctc tgtggcgtca atggataaca ctcgacccgc taaagccacc
     3721 gaaccgttgt tcaggatgaa atacttcgtt tttgctgggt taaactcggt aaaaataaaa
     3781 gtattttgtt ggtctttaac ggatttaatt acgtgagtta aattctttgt actgtttatt
     3841 atgccagttt gttgtttttg aatagtttcg agttgactct ttagctgtgc ttgttgtttt
     3901 tcaagtagca caattttatt attatgttct tcttgttgtt tcagtaaact actttctggc
     3961 ttgccttctt caatgtagtc acatcccgtt aatgcgcaga gaactgaaac agtgacagca
     4021 ctgaaaaaaa tcttcttcaa gttcatgtaa aaatccttat ttcctccgac cagcgatcaa
     4081 tgccggtaga gttgtccatt gtattattgg tgagctatgt tgctttacat taatgacagt
     4141 gatgtgtgtc gattaaatat tgttttaagc attcatcata aaaatgtatc attcgtttca
     4201 aaagatgtct gtgtttgtat gaatatatta tttttcgtgg attctaatga tgattattaa
     4261 taaatgtcaa atatgaaaga aaccatgtct gggaaaaatt ttacagataa ttgattttgt
     4321 gaatatgatg attaaaattt cacaattaaa tccgaaaaaa agagacgtaa a
//
LOCUS       DADKXM010000214         4361 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.217, whole genome shotgun sequence.
ACCESSION   DADKXM010000214 DADKXM010000000
VERSION     DADKXM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.217"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..95
                     /locus_tag="JQR05_003862"
     CDS             <1..95
                     /locus_tag="JQR05_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY5325856.1"
                     /translation="LINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            356..922
                     /locus_tag="JQR05_003863"
     CDS             356..922
                     /locus_tag="JQR05_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY5325857.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLLRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            926..1501
                     /locus_tag="JQR05_003864"
     CDS             926..1501
                     /locus_tag="JQR05_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY5325858.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1543..2526)
                     /locus_tag="JQR05_003865"
     CDS             complement(1543..2526)
                     /locus_tag="JQR05_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY5325859.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPDALFIDMRNHYEYEVGHFENALEIPADTFREQLPK
                     AVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLP
                     VRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKY
                     KGCCSEICCEESALPPEEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
     gene            2751..3671
                     /locus_tag="JQR05_003866"
     CDS             2751..3671
                     /locus_tag="JQR05_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY5325860.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RITRWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3843..4040
                     /locus_tag="JQR05_003867"
     CDS             3843..4040
                     /locus_tag="JQR05_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY5325861.1"
                     /translation="MSPCENDIPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            4064..>4361
                     /locus_tag="JQR05_003868"
     CDS             4064..>4361
                     /locus_tag="JQR05_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY5325862.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKVLVKAGDTLLIESLDRLSRQDIDIASE"
BASE COUNT         1092 a         1055 c         1025 g         1189 t
ORIGIN      
        1 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
       61 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
      121 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac
      181 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
      241 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
      301 ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc
      361 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
      421 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
      481 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
      541 gctggttatt cgcgttctgt tgcgtgtcat atctccaccg ccgggtccgc tgccgagtta
      601 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt
      661 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
      721 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
      781 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat
      841 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
      901 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
      961 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
     1021 gcacgccttt gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt
     1081 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa
     1141 tgtgacaatt aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg
     1201 cagtgcggat tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt
     1261 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
     1321 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg
     1381 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
     1441 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
     1501 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
     1561 aatacccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
     1621 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
     1681 ctcactacaa caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg
     1741 gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg
     1801 cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
     1861 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
     1921 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
     1981 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
     2041 gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa
     2101 gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc
     2161 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
     2221 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
     2281 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
     2341 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
     2401 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
     2461 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
     2521 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
     2581 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
     2641 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
     2701 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
     2761 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
     2821 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
     2881 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
     2941 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
     3001 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
     3061 tcacccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
     3121 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
     3181 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
     3241 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
     3301 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccggat catgattacg
     3361 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
     3421 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
     3481 agccagatgg caaagggtat caattgatta tgctgccgcc agagtgttct ccgccactgg
     3541 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
     3601 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
     3661 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
     3721 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
     3781 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
     3841 ctatgtcacc ctgtgaaaat gacatcccta taaactggaa acgaaacctg atcgtcgcct
     3901 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
     3961 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
     4021 tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag
     4081 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
     4141 gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt
     4201 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
     4261 tttaaccgct atcgagaaag tgcttgttaa agctggtgat acgctactaa tcgagagttt
     4321 agaccgtctg tcacgtcagg atattgatat tgcttctgag c
//
LOCUS       DADKXM010000215         4334 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.218, whole genome shotgun sequence.
ACCESSION   DADKXM010000215 DADKXM010000000
VERSION     DADKXM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.218"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..553
                     /locus_tag="JQR05_003869"
     CDS             <1..553
                     /locus_tag="JQR05_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5325863.1"
                     /translation="QVALPAMTVLLGKTTLTTPAGREGLSPRVSYGKGTEGFSLVWTA
                     PGMAEVTLPVTGDNNARAGTFVFRIQAVGVLRHVQGGQPVYVGMYDDLNTNGLPGEST
                     AMKVSDIPGVLQKMFSGEGPGWLQTMTLSGYSGVSHFSDDFLRQVEGVYGAQTVAGSG
                     ELRLNGEMPEHWRVSLSVNIEYQ"
     gene            878..2104
                     /locus_tag="JQR05_003870"
     CDS             878..2104
                     /locus_tag="JQR05_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY5325864.1"
                     /translation="MSGTIPKIKTGENVLSAAIQRIEWLFGTFPSVCLSFSGGKDSTV
                     LFHLVSEVARKKRRRFSVLFIDWEAQYQCTITHIQKMQKMYLDVTDRFYWVALPLTTV
                     NGVSQFQPEWICWEPGVTWVRHPPEYAITDMAYFPFYRYAMTFEEFIPAFSSWFARDK
                     YLVAVLTGVRADESLNRFMGLVSRRKQRYADDKPWTTASPEGFYYTMYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMHRACVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            2089..2727
                     /locus_tag="JQR05_003871"
     CDS             2089..2727
                     /locus_tag="JQR05_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY5325865.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            2955..3347
                     /locus_tag="JQR05_003872"
                     /pseudo
     CDS             2955..3347
                     /locus_tag="JQR05_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3366..3839
                     /locus_tag="JQR05_003873"
     CDS             3366..3839
                     /locus_tag="JQR05_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325866.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            3991..4253
                     /locus_tag="JQR05_003874"
                     /pseudo
     CDS             3991..4253
                     /locus_tag="JQR05_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904911.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1169 a          862 c         1109 g         1194 t
ORIGIN      
        1 tcaggtcgca ctgccggcga tgaccgtttt actgggaaaa accaccctga ccacaccggc
       61 aggacgtgag gggctttcac cccgggtcag ttacggaaaa ggcactgagg ggttttcact
      121 tgtatggaca gcgcctggca tggctgaagt gacgctacct gtcaccggag ataataatgc
      181 ccgggcaggc acatttgttt tcagaattca ggctgtgggg gtgttgcgcc atgtgcaggg
      241 cggacaaccg gtgtatgttg gtatgtatga cgacctgaat acgaatggtt tgccaggtga
      301 aagcactgcc atgaaggttt ctgatattcc gggcgttctg cagaagatgt tcagtgggga
      361 aggccccggc tggcttcaga caatgacact cagtggttat tcaggggtga gtcatttcag
      421 tgatgacttc ctgcgccagg ttgaaggcgt gtatggtgca cagactgtgg ctggcagtgg
      481 tgaattacgg ctgaacggcg agatgccgga acactggagg gtgtcactgt cagtaaacat
      541 tgaatatcag taaggagcag gagtgcgttt tataatccgg agataaggaa cgtcggttac
      601 tgtaagattg gggagttgtg ttgatagcgg gagtggctgg aataagtggt atacatagat
      661 aaaaattaaa actatcacta tttgagtgag ttttttcctg ctctccatgg gctttatagc
      721 tcacaaaaaa tagaataata ttattaatta ttctgctttt ttatgggcat tcccccttga
      781 aaaatgagtt tgtgccagaa tattattaat atgttttttg tgatttacta atcaaggatt
      841 gctgaatagc atccagtact gaatattttg ttcaattatg tcaggaacca taccgaaaat
      901 caaaacaggt gaaaatgttc tgtctgcagc cattcagcgc attgaatggc tatttggaac
      961 gtttccttct gtctgcttgt ccttttctgg tggtaaggat tctaccgtgc ttttccatct
     1021 tgtgtccgaa gtggcccgca aaaagaggcg tcgtttttcg gtattgttca ttgactggga
     1081 agcacaatat caatgcacca ttacgcatat tcagaagatg cagaagatgt acctggatgt
     1141 gacggacagg ttttactggg tggcccttcc cctgactacg gtaaacggtg tctctcagtt
     1201 tcagccggag tggatatgct gggagcctgg tgtgacctgg gttcgccatc caccggaata
     1261 tgctattacg gatatggcgt attttccctt ttaccgatat gccatgacct ttgaggaatt
     1321 tatcccggcg ttttcctcct ggtttgccag ggataaatac ctggtggctg ttctgaccgg
     1381 agtccgggca gatgagtccc tcaaccgctt tatgggactg gtatcccgtc gcaaacagag
     1441 atatgccgat gataaaccct ggactacggc atcacctgaa gggttttatt acaccatgta
     1501 tccgttgtat gactggaaag cccgtgatat ctggatatat aacgccagaa cccgagctat
     1561 ctacaatccc ctgtatgacc tgatgcaccg tgcctgtgtg ccgttacgca acatgcgtgt
     1621 ctgtgagcct tttggtcctg aacagcgtaa ggggctgtgg ctttatcatg ttctggagcc
     1681 ggaaacctgg gccaggatgt gtgagcgggt gtcgggtgct gccagcggtg cgctttatgc
     1741 caatgagagc ggtgcctatt ttgccctgcg taagcgtatc acaaagccgc ctcatcatac
     1801 ctggcgtagc tatgcgatgt tcctgctgga tgtgatgccg gaaagaacgg cagaacatta
     1861 ccgtaataaa attgctgtct acctgcgctg gtatcagacg cggggcttcc cggatgacat
     1921 cccggatgaa caggagaatg acctggggag ccgggatatc ccgtcctggc gacgtatctg
     1981 taagacactc ataaagaatg atttctggtg ccggactctc tccttcagtc cgaacaaacc
     2041 ccggcactat gaacgttacc tgcagcgtat gaaggaaagg aggaaggaat gggggattct
     2101 gtgacaccgg agatacttgg caacatgatt cgtcagtatt tcagtcaggt acgaactgaa
     2161 aaagagagca tacaggctct gaatcatctg cgcagagtgc ttcatgaggt cagtcctttt
     2221 gcacaggagc ctgtggactg tgtgttgtgg gtgaaagcgg atgaggtggt ggccaacgat
     2281 tacaacccta atgtcatggc actaggtgag aaaaaactac tgaaacagtc tctggagaaa
     2341 gatggtttca cacaaccggt ggtggtgtca gaggagaaga atcattatct ggtggtggat
     2401 ggttttcatc gtcagttact ggggcgggaa gcagatacag gaaaacgcct gaggggctgg
     2461 ttgcctgtag cctgtattaa tccggagaga aagggacagg ccgcacgtat tgccgcgacc
     2521 attcgacata accgggccag ggggaaacac cagatcactt taatgtctga tattgtcagg
     2581 gatctttcac gtctgggctg gacgaatgaa cgtattggta cagagctggg aatggaccag
     2641 gatgaagtgc tgaggctgaa acagattagc gggctgacag agttgtttca ggaagaggat
     2701 ttcagctcgg cttggacggt tcgttgatgt tgttaaatat gggcggtatt tctgaaagtt
     2761 gatactttgg gtagaaaaaa tgataccgaa aactatggag gatgtgcttg tcaggtgact
     2821 ggacaccata ggtcttaggc tttgataacg ggataagatg agctgctttt ctcataccat
     2881 caggtgtgat gatggcgcag atttacaaaa tctgattgat tctaaagatg tataagtaaa
     2941 aatcaagctt tgatatgaaa aaacagatat ttattaataa taaacctcct gtagtccctt
     3001 actcagggac gcacgcgaaa atatttaaat acattgaaat ccctctgccg tttttctact
     3061 ttatctatac atcaggcgaa ccattccaca tatctgtcca aaatactgtt atttatgtat
     3121 caaaatacaa tggtatattt attaataaat tagttccttt ttctctttta tttgacaggg
     3181 atatatctgt tcttcaaaga agagatattt gtgttgtgcg atttacgtca gaggaaataa
     3241 gtgaacataa cgttttattt gatcatgaca ttgaacgatt aaaaaaaatt tccaaagctc
     3301 agcttatatc tcccgattat gttttgattg atttttctag tgggggggga gatgaaccct
     3361 atgcaatgtc ctggttaata tctcagtgtg ctcatcagtg cactgataat aaaaagacgg
     3421 agacagatgc catttacgat aaagttcgga gttcttattt gctttcatgt attctgaaaa
     3481 aaaacaaaaa tgttgggctt attcttcatg caccatcatt tgtttctgtg tctgaaaaaa
     3541 ttgccagaat tgtgatggca aattactccc gtaactggag taattccgag ttggccagtg
     3601 cggttttaat gagtgaatct tctttaaaaa gaagaatgta caaagaggtc ggttctataa
     3661 gtacatttgt acataaaata aagcttacag aagctatcag gaaattacga aggacaaata
     3721 ctcccatttc tgtcatatca tctgagttag gttacagttc accatcgtat ttttctaaag
     3781 ttttctttaa atatttaaag acataccctc aaaacataag gaagaaaaat ggtcggtaat
     3841 gtcaccaatt aggaggactt gcagagccgc ttttttacga tactcacccc gtaagtaagc
     3901 gataattggt aacttaaacg cccatctgac atagatggac acctgagtat ggaattacag
     3961 gactggcgaa aagaacctcg taaaaagtat ttggtgcaaa tcgttggttc ctttgctgaa
     4021 tttgaacgcg ccatgcttcg tgaacggaca aagagtggac ttgcagcggc acgccaggac
     4081 gggttggtgg atggcgacta aagttaacac cccagcaaca aaaagagatt gtctcgctcg
     4141 tcacatcggg gcagaaaaca ggtgctgatg ccgcccgtct gtttcgggtt cacccttcca
     4201 ccgtggtgcg tcttctcgcc agacaccgga tgactgagat caaacagaca taatctatgc
     4261 ggagatttac agcagggcat ggttcacaaa tctgacagcg atgaattatc ggcactgtgg
     4321 gcagaaaatg ctcg
//
LOCUS       DADKXM010000216         4266 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.219, whole genome shotgun sequence.
ACCESSION   DADKXM010000216 DADKXM010000000
VERSION     DADKXM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.219"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..361
                     /locus_tag="JQR05_003875"
     CDS             <1..361
                     /locus_tag="JQR05_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325867.1"
                     /translation="ARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQ
                     ILQKSPVFSLGNPIHDSEQKIIYAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVP
                     VLLSNVLIARLAAELLV"
     gene            433..717
                     /gene="ppnP"
                     /locus_tag="JQR05_003876"
     CDS             433..717
                     /gene="ppnP"
                     /locus_tag="JQR05_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY5325868.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            872..1207
                     /locus_tag="JQR05_003877"
                     /pseudo
     CDS             872..1207
                     /locus_tag="JQR05_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            1204..1659
                     /locus_tag="JQR05_003878"
     CDS             1204..1659
                     /locus_tag="JQR05_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325869.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKEFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(1737..2648)
                     /gene="rdgC"
                     /locus_tag="JQR05_003879"
     CDS             complement(1737..2648)
                     /gene="rdgC"
                     /locus_tag="JQR05_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY5325870.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            2773..3681
                     /gene="mak"
                     /locus_tag="JQR05_003880"
     CDS             2773..3681
                     /gene="mak"
                     /locus_tag="JQR05_003880"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY5325871.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGILGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAVGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3824..>4266)
                     /locus_tag="JQR05_003881"
     CDS             complement(3824..>4266)
                     /locus_tag="JQR05_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="member of the major facilitator superfamily (MFS)
                     of transporters; unknown function; may be associated with
                     transport or processing of arabinose polymers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY5325872.1"
                     /translation="VLGNMLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCGGMKTTS
                     LIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTL
                     GLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPLG"
BASE COUNT         1064 a         1030 c         1104 g         1068 t
ORIGIN      
        1 tgcgcgtaat acgatctttc ttgagccgtc gcgaatattg cctccactgg tttcctctat
       61 tgttgaagat catcaggtgg gggttatcgt tccggttgag gagatgctgc ccgttcaggc
      121 gcaaaaatgg caaattttgc agaaatcgcc ggtattttca ttgggtaacc ccattcatga
      181 ttcagaacaa aaaatcattt atgctgggaa agaattactg gcaaaagggg ctgatgtcat
      241 catgctggat tgtttaggat ttcaccaacg tcatcgtgat ttactgcaaa aacagctcga
      301 tgttcctgtc ttgctctcta acgtattgat tgcacggctg gctgcggaat tactggtgta
      361 attttgcgtg acagccagcg cctctggccc ctatagtgaa gtagatgttc aactaccaga
      421 cagggccagt ttatgcttca aagtaatgag tacttttccg gcaaagtgaa atcgatcggc
      481 ttttccagca gcagcactgg tcgcgccagc gtgggtgtta tggttgaagg cgaatacacc
      541 ttcagcaccg ctgagccgga agagatgacg gtaatcagtg gcgcgctgaa tgtgttactg
      601 cctgacgcga ccgactggca agtgtatgaa gccggttcgg tgtttaatgt tcccggtcac
      661 agtgagtttc atcttcaagt tgccgaaccc acctcttatc tgtgccgcta tctgtaattc
      721 ctcgccttcc ccttgaacgg gagggcattt ttctgaaata tcctctcttt aacccataat
      781 aatatttcct ttgctgcgat tttttcaatt tccgatatat tcataattca tcaaggttga
      841 tataaatatc agtgaagatc tccaggtatt ttttgcggaa ctggctacga taaaagataa
      901 atcagatgat gaatggtggc gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac
      961 ggcattacgc aacgcacata ccccggcgtc gttgttaacg acattgactg agcctcagga
     1021 tcggtcgctg gctattaata atccacagct ggctgccgat gtgaaaacgg cgtggttaaa
     1081 agaggatcca tcattactct tatttgtcga acaacccgat ctttcgcagt tacgtgattt
     1141 agtgaaaacc ggggcaacgc ggaaaattcg cagtgaagcg cgtcactggc ttgaggaaaa
     1201 acaatgacac aacgtccatg gagcaagtta cagcgggaga tctatgactt actgacgcca
     1261 acgatcaacc tgcaaattca ctgtacccgt tatcccatgc gcagccaaaa tggtggaagt
     1321 accgatttac cgcgttattg gatcacgttg gataaaaacg tgatctggga ttacccgaaa
     1381 gaatttattg caggtaacgc tggagtgcgt aattttcacg gtgaaacttg ctggtatcct
     1441 tacctgacgg atatttgttc aatttctgat ttattgcgtg aatatattga taccccaaaa
     1501 gcggagctgt taacaaaaca atttaccagc gataaatggg ggttagtgaa tattctgcgt
     1561 gctgctgaca ggcgaatagg gatgaggcga ttagatcagt tacggcgtaa aacgcataat
     1621 attgctgcgc taaaaattat tgctcgccgt agcgaataat tatatgcctg gtgtggcttc
     1681 gtacgccgga taagacgcgg caggcgtcgc atccggcatt aaaggaaaat cagcaattaa
     1741 cgttgtgctt cgccacctaa tccttcaatc aggttttgaa ttaacgctgc cagttcacca
     1801 gtcatcagga tgaaatcggc atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg
     1861 ttttgatcgc gcagctcgtc gcagaactta agacgcttga gcgaaccatc gtcgcacatc
     1921 acaaactgaa tgcgctgctg ccagtcgagc gccagtttag tcaccacttt tccggcttca
     1981 atgtgattgg tgatctcttc gctggtcaga tcttgtttct tcgcgcggat cacgccgcca
     2041 tcttccagca acgatttcag ctcggcttca tcaagcagct ggaagccctg tgccgcacta
     2101 ccggagcgaa cccattcggt cagcgtcagt tcaatcgggt tttccatgct caatggtaca
     2161 accggtaacg accccaggct tttacgcagt aatgccagcg tatcttccgc ttttttggca
     2221 ctggcgcagt ccaccataat caaaccgtta accgtgtcga gccacatcat tgtctggcta
     2281 aaacggctga aagcacgcgg cagcagagag tgcagcactt cgtctttcag cgaatctttt
     2341 tcggttttct tcagcttacg cgcctgttcc gcttccagtt tggcgatttt cgcttccagc
     2401 gcctgtttaa tcaccggaga cgggaggatt ttttcttctt tgcgcgcgca gataacaatt
     2461 tgaccattgg caacgtgcgt taacgcatcg ctgtgcgatc ccatcggagg aacccagccc
     2521 atcttcgcca tgtcctggct gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc
     2581 atctcttctg cacgcagcga aatctcgcgg ctaagacggt aaaccattaa atttttgaac
     2641 cacagcatga taatttccac ggccttgtcg ttaaatttag cgggcatgat aacgaattgt
     2701 cggcggcctt gcattgccaa tccggttgtc cgtctctacg ctattgatat tgaaaaaaat
     2761 aaggagagta cagtgcgtat aggtatcgat ttaggcggca ccaaaactga agtgattgca
     2821 ctgggcgatg caggggagca gttgtaccgc catcgtctgc ccacgccgcg tgatgattac
     2881 cggcagacta ttgaaacgat cgccacgttg gttgatatgg cggagcaggc gacagggcag
     2941 cgcggaacgg taggtatggg cattctaggt tcgatttcgc cctacaccgg tgtggtgaag
     3001 aatgccaatt caacctggct caacggtcag ccattcgata aagacttaag cgcgaggttg
     3061 cagcgggaag tgcggctggc aaatgatgcc aactgtctgg cggtttcaga agcagtagat
     3121 ggcgcggcag tgggagcgca gacggtattt gccgtgatta tcggcacggg atgcggcgcg
     3181 ggcgtggcat tcaatgggcg gacgcatatc ggcggcaatg gcacggcagg tgagtgggga
     3241 cacaatccgc taccgtggat ggacgaagac gaactgcgtt atcgcgagga agtcccttgt
     3301 tattgcggta aacaaggttg tattgaaacc tttatttcgg gcacgggatt cgcgacggat
     3361 tatcgtcgtt tgagcggaca tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa
     3421 agcgatccgg tagcggaact ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg
     3481 gcacatgtcg tgaatattct cgatccggat gtgattgtcc tggggggcgg gatgagcaat
     3541 gtagaccgtt tatatcaaac ggttgggcag ttgattaaac aatttgtctt cggcggcgaa
     3601 tgtgaaacgc cggtgcgtaa ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg
     3661 tggttatggc cacaagagta aaaaacgtag gcaattggcg catcatgcct gatgcgacgc
     3721 ttgccgcgtc ttatcaggcc tacaaaatgt gccagaaccg cgtagggcgg ataaggcgtt
     3781 cacgccgcat ccggcaataa gcaacgcgca ccgtgacagt aatctacccc agtggtttcg
     3841 ccagcaccgg actatccgcc gcttgctggc gcttatagcg accatacagc agcaacgatg
     3901 acatcgcagc aaacgaaagc agggcggcag gcagcgccac gtaattatat gccagcccca
     3961 gcgtcagcat catacctccg caatatgcgc cgacggcgct accgaggtta aacgctattt
     4021 gcccacctgc ggcacctaat aactctccgc ctttggcatt ttgcagtaac aatatttgca
     4081 gaggtgctga aagggcaaat aatcccgcgc aacaaataaa agcaaaaata agcgacgttg
     4141 ttttcatgcc gccgcagaaa aagagcatca gcagtgccag tacaatgata aagtcagtca
     4201 ctgctgcaat gcgcagtggt gaataacgtc ctgaaatcct gccacttagc atatttccca
     4261 gcacca
//
LOCUS       DADKXM010000217         4217 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.220, whole genome shotgun sequence.
ACCESSION   DADKXM010000217 DADKXM010000000
VERSION     DADKXM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.220"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JQR05_003882"
     CDS             complement(<1..209)
                     /locus_tag="JQR05_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5325873.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JQR05_003883"
     tRNA            complement(324..399)
                     /locus_tag="JQR05_003883"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JQR05_003884"
     tRNA            complement(406..480)
                     /locus_tag="JQR05_003884"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JQR05_003885"
     tRNA            complement(597..681)
                     /locus_tag="JQR05_003885"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JQR05_003886"
     tRNA            complement(690..765)
                     /locus_tag="JQR05_003886"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JQR05_003887"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JQR05_003887"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY5325874.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQR05_003888"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQR05_003888"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY5325875.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQR05_003889"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQR05_003889"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY5325876.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAVVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1090 a         1049 c          940 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaac ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 cctgacggat ggccttt
//
LOCUS       DADKXM010000218         4212 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.221, whole genome shotgun sequence.
ACCESSION   DADKXM010000218 DADKXM010000000
VERSION     DADKXM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.221"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(32..3079)
                     /gene="fdnG"
                     /locus_tag="JQR05_003890"
     CDS             complement(32..3079)
                     /gene="fdnG"
                     /locus_tag="JQR05_003890"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2492..2494),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY5325877.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            3311..4192
                     /gene="yddG"
                     /locus_tag="JQR05_003891"
     CDS             3311..4192
                     /gene="yddG"
                     /locus_tag="JQR05_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY5325878.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGVHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSALLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT          927 a         1145 c         1064 g         1076 t
ORIGIN      
        1 cctgcgtttc catagccatc tgttcgcccc cttacgcctt ctcgatgttg actaagaacg
       61 ctttatattc cggcgtttgc gagtttgcat caccgacatt cggcgtcaga gtgttagcga
      121 tataaccttt acgcgcgaca ccctcaaagc cccagtggat tggaataccc accgtttcaa
      181 cctgctgacc gtttacattc agcggtttca gacggcgcgt taccactgcc accgcgcgga
      241 taaagcctcg cttgctggag acagtgacac gatcgccatt attaatgcct ttcgccgccg
      301 cctgcgtttc gctgatttcc acaaactgtt ccggctgagc aattgcgttg agcaacgcgt
      361 gcttggtcca ggtgtggaag tgctcggtca gacgataggt cgtacccaca tacgggaact
      421 gctctttttt acccatccgc agcgcgtctt gttcatacag acgaacaacc gggttagaca
      481 ccacgttcgg atgcagcggg ttagtgccca gcggcgtttc aatcggctcg tagtgttccg
      541 ggaacggacc ttccgccatt ttgttgatgg caaacaggcg tcccatccct tccggctgca
      601 taataaacgg cccggttggc gtacccggtg cggcattgcc gaagtccgga atatcgttac
      661 ccgtccactt actgccgttc cactggatca gcatccgttt cggatcccac ggtttaccgt
      721 tgatatccgc cgaagcacgg ttgtagagta cgcgacggtt gagcggccac gcccaggccc
      781 atcccagcgt attccccaga ccggacgggt cggagttatc gcggtttgcc atctggttac
      841 cctgctctgt ccagctaccg gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac
      901 gcagatgcgc aaagctactc agcaactgac ctttcttcgc aatcagcacg ccattagcat
      961 catagagatc ttccaacgca tagccgttgt tctctttagc tacttcgtca gattgcggtt
     1021 cgtgcggctg cttgtagttc cagctcatct tcatcagcgg ttctacgcct ttaccacctt
     1081 cggcctggta caactcgcgc agatgatggt agatacccgc cagaatttcg ccgtcgttac
     1141 gcgcttcgcc cggcgcgtcc tgacctttcc agtgccactg caaccagcga ccggagttag
     1201 cgatagaacc atcctcttca gcaaagcagg tcgaaggcag acggaatact tcagtctgaa
     1261 tagacgccgg atcgacatcg ttcgactcgc cgtggttctg ccagaaggta gaggtttcag
     1321 tcaccagcgg atcgataacc accatgtact tcagtttgct caggcagctc accactttgt
     1381 ttttatccgg gaaggacgca accgggttaa agccctggca gaaataaccg gtgactttgc
     1441 cttcatccat catgttgaaa tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc
     1501 agtcatagcc ccagttgttc tctttctgcg cggcatcgcc atagaaagat ttcatcaggc
     1561 taacgaagaa cttcggatag ttgctccagt agttcacctg atcagccagc gtcgctttcg
     1621 gcgtgttcgc ttccagatac gactgcaaat caacctgttt ttctgacggc agcgtcagat
     1681 aacctggcag gctggtagag agcagaccta agtcagtcag cccctgaatg ttggagtgac
     1741 cacgcaatgc gttcacgccg ccaccggcca tacccatgtt accgagcagc aactggatca
     1801 tcgccatagt acggatgttc tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca
     1861 ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag cacttcacac actttcagga
     1921 agtcggcttt tggcgtacca cagatgtttt caacaacgtc cggcgtgtag cgggaaacgt
     1981 gcgctttcag caggttccac acacagcgcg gatgagtcag tgtttcatcg cgtttcgcat
     2041 agccgttttc atcgaactga tagttccagg acgatttgtc gtactggcgt ttttcagcgt
     2101 cataaccgct gaacaggcca tcttcgaaag caaaatcatc acgcaccagc aggctggcgt
     2161 tggtgtaatg tttaacgtat tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa
     2221 cgccagacag gaacgtaatg tccgtaccgg aacgaatagg tgcgtaaata tccgccacag
     2281 aagcggtacg cgtaaaacgg ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg
     2341 cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc agcgttaccg cccattacca
     2401 ttacgacgtt agcgtttttg atatccaccc agtggttggt catagcaccg cgaccaaatg
     2461 ttggagcaag acttgctacc gttggtccgt gtcagacgcg cgcctggttg tctaccgcca
     2521 gcatcccgag ggagcgggca aatttctggg tcagcatccc ggtttcgttg ctggcaccgg
     2581 aggcacacag cataccggta gaaagccaac ggtttaccgt tacgccctgc tcgttctttt
     2641 caataaagtt agcgtcacgg tcagctttca tcagcttcgc aatacgggag aatgcttctt
     2701 cccagctaat gcgctgccat ttgtcagaac ctggcgcacg atattccggg tagcgcagac
     2761 ggttttcact gttgacgtaa tccagcaaac cggccccttt cgggcacagc gcaccacggc
     2821 ttaccggatg atccgggtca ccttcaatgt gataaatcgc ttctctggcg ttttttgcgc
     2881 catcacccag gctatacatc aatagcccgc aacctacgga acagtatgtg caggtgttac
     2941 ggatctcttt agcgcgtaat aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg
     3001 caaagcccaa tgccgctacc gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt
     3061 gtctgcgact gacgtccatt gctttcctct tttttcaggg gtattactgc gcgaggaaac
     3121 taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa gaacacgtgt ttgccacaaa
     3181 ataatccgcc tgcgcaaatt gacctacctc aatagcggta gaaaaacgca ccactgcctg
     3241 acaggccagt taaaaaaatg ctataaaatt cagcttaatt tttaacggca agagagacaa
     3301 aacagcgagc atgacacgac aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg
     3361 gagcacgatg gtaggattga ttcgcggtgt cagcgagggg ctcggcccgg tcggcggcgc
     3421 agctgctatc tattcattaa gcgggctgct gttaatcttc acggttggat ttccgcgtat
     3481 tcggcagatt ccgaaaggct atttactcgc cgggagtctg ttattcgtca gctatgaaat
     3541 ctgtctggcg ctttccttag ggtatgcggc gacccgtcat caggcgattg aagtgggtat
     3601 ggtgaactat ctgtggccca gcctgacaat tctctttgcc attctgttta atggtcagaa
     3661 aaccaactgg ctgattgtac caggattatt attagccctg gtcggcgtct gctgggtgtt
     3721 aggcggtgag aatggggtac attatgatga aatcatcaat aatatcacca ccagcccatt
     3781 gagttatttc ctggcgttta ttggtgcgtt tatctgggca gcctattgca cagtaacgaa
     3841 taaatacgca cgcggattta acggaattac cgtttttgtc ctgctaacgg gagcatgtct
     3901 gtggatttac tattttctca cgccacaacc agaaatggta tttagcacgc ccgtcatgat
     3961 taaactcatc tctgcggcat ttaccttagg atttgcttat gctgcatgga atgtcggtat
     4021 attgcatggc aatgtcacca taatggcggt aggctcgtat tttacgcctg tactttcctc
     4081 agcgcttgca gccgtgctgc tcagcgccct gctgtcgttc tcgttctggc aaggcgcgct
     4141 aatggtctgc ggcggttccc tgctctgctg gctggcgaca cgtcgtggtt aaaataattg
     4201 ccgggtctac gg
//
LOCUS       DADKXM010000219         4200 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.222, whole genome shotgun sequence.
ACCESSION   DADKXM010000219 DADKXM010000000
VERSION     DADKXM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.222"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            153..905
                     /locus_tag="JQR05_003892"
     CDS             153..905
                     /locus_tag="JQR05_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY5325879.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VVVQPAIKGYEPDSNGINQSTTLPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            905..1258
                     /locus_tag="JQR05_003893"
     CDS             905..1258
                     /locus_tag="JQR05_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325880.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVRFST
                     EIDTLYGITTVTSEA"
     gene            1258..2457
                     /locus_tag="JQR05_003894"
     CDS             1258..2457
                     /locus_tag="JQR05_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325881.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDGNNVIWRLPASVVVGVDGTVMATARCSVSGAVAALA
                     GTITEINTPTRGWVSVINPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVAGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQVISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGWNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            2454..3134
                     /locus_tag="JQR05_003895"
     CDS             2454..3134
                     /locus_tag="JQR05_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY5325882.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSCELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGILL"
     gene            3134..3478
                     /locus_tag="JQR05_003896"
                     /pseudo
     CDS             3134..3478
                     /locus_tag="JQR05_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3869..>4200
                     /locus_tag="JQR05_003897"
     CDS             3869..>4200
                     /locus_tag="JQR05_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5325883.1"
                     /translation="MDVYANGQHVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYG"
BASE COUNT         1070 a          886 c         1133 g         1111 t
ORIGIN      
        1 aaaaaaagaa gaatgtagat tggtgctatc tgaaaataag aaatgagttc gctaaattaa
       61 cttttcacgg taattgcttc tatcaccatg gaaagaacta gctacataaa acccgccttc
      121 gagtgggttt tttgctttct ggaggcatat gaatgccagt ttctttaagc gctcaacttg
      181 gtagcaaaga acaggccgat gtaagacttg ctggctctgt catgtcggcg ctgcgtgttt
      241 ctatgcccgg catcgtccag tcatttgatc cggacacggt aacggtagtt gttcaaccag
      301 cgattaaagg ctatgagcca gactcaaatg gaatcaatca gtcgacgaca ttacccttgc
      361 tggtggatgt gccagtggta ttcccgcgcg gcggaggctg tacgttgact tttccggtaa
      421 aagccgggga tgagtgtctt gtcgtttttg ccgatcgttg tattgatttc tggtggcaga
      481 gtggcgggat acaggagccg gttgatgaca gaatgcatga tttatcggat gcgttttgta
      541 ttgtaggtcc ccagtcgcag gcgaggaaga ttagcggtat taataccagt gccacacagt
      601 tgcgtagtga cgacggcagt acctattttg agcttaatcc tgataccagg aaaattaaaa
      661 ttgtcgctcc gggtggtctt gatgtggttg cccctctggc tgatttttct gagaaagtaa
      721 ccattcatgg cctgttaacc tggatgggtg gcatggtggg gtctgttgtt tctggtgtgg
      781 cttcaaaaat cactggtgct gttgagtttt tgggtagcgt gaaggctaac ggcaagccaa
      841 tcgatgatac gcacactcat ggtggtgttc agcgcggtgg aagcaatacc gacggagtaa
      901 actgatgcga tacagacgtg aagacgccga tggcgattac acctttggca gcggtgatga
      961 cacctggctg attaactcac cggaggccgt ggcgcaggcg gtaaaaacgc gattcgaatt
     1021 gtggtatggg caatggtttc tcgacaccac cgaggggact ccgtggatcc agtccgtact
     1081 cggtaagcag aagccggaaa cctacaacct ggcgatccgt aagcgcatcc tcgaaacgcg
     1141 gggcgttaaa tcaatcctct ctttcaatac tacggtggat accacgaccc gacgtgtcag
     1201 gttttccact gaaatcgaca ctctctatgg aataacgact gttacatcgg aggcgtaatg
     1261 gctctgaacc ttgattctct cggtttatct gcaaaggtaa ccgcggaggg gatcagtgcg
     1321 cctgattatc agacgatact cagcaccctg attagctatt ttcagcagat ttatggtagt
     1381 gatgcctacc tcgaaccgga cagtaaagac ggtcagatgg tggctctgat ggcgctggcg
     1441 attcatgatg ccaataatat ggctataact gtctacaact gtttttcacc ggcaaccggc
     1501 tatggggctg cactgaccag taacgtgaaa ataaatggta tttcacgtaa aggcgcgacg
     1561 aactctacgg ttgatttgct tcttacagga acagccggaa caaccatcat taatggcagc
     1621 gtgaaagatg gtaataatgt gatatggcgt ttgcctgcgt cagtggtggt cggtgtggat
     1681 ggtacagtga tggcgaccgc aagatgttcc gtcagtggtg cagtggcggc gctggctgga
     1741 actatcactg aaattaatac gccaacccgt ggctgggttt cggtaattaa tcctgctgcg
     1801 gctactgttg gctctccggc agaaactgat gctgagttac gtatccgcca gtcgcaaagt
     1861 gttgcgttgc catcaataac cccatttgaa gcactggatg gtgctgtttc taatgttgcc
     1921 ggtgtaaccc gccacaaact ctatgaaaac gatactggtt cggaggacgg taacgggtta
     1981 ccgccacact ctgttgctgt aattgtggat ggcggtgatg tgacggatat tgctcaggct
     2041 atcagaggga ataaaggcca ggggacagcc actcacggta caacatccgt tacggttccg
     2101 gataaatacg gcaatcccca tgtaatcaaa ttctcgcgtt ccagtgatgt gcctgtttat
     2161 gcccggatta aattaaaagt ttttacgggt tatacctcac agatagggca gcagatccag
     2221 caggttattt ccgactatat caatagtctg acgattggtg attcggtcct tttaagtcgc
     2281 atttactcac cggcgaatct tggcgtggtg agtggctgga atgcacgcta ttacgatatt
     2341 caggagctga cgattgggaa atccccgggg gctttgtcgt catcaaacat tgatatcaga
     2401 tacaacgaat ctgcgtcctg taccccggaa aatatcgtta taacggtgga gtcatgagca
     2461 aatacaccga actaatcacg aactaccacg ccaccaaacc taaatttctt gcgcatgttg
     2521 atctgatgac ccggccactt attgatgttg cggctgccac cagagggctg attactgcat
     2581 ttgatattga ctctgcggtt ggtgtgcaac ttgacattct gggattgtgg atcggacgta
     2641 gccgtgttgt cagccagcct atctcaggtg tctatttcag ctgggatacc gacgggcttg
     2701 gatatgatca gggggtatgg cagggaccat acgatcctga ttccggatac atgtacctca
     2761 gcgatgaaac ttatcgtgtc attcttaaag cgaagattgc gattaataac tgggatggac
     2821 ggaatgattc gcttccggca attcttgacg ctgcaacagc aggatccggg ctgcgaatgc
     2881 agatagtcga taaccaggac atgacgatat cggtctgggt ctttcctgat actgatattt
     2941 cagatgtatc gtgtgagtta atagcggcaa ttaaacaggg gtatctcaca gtaaaagccg
     3001 ccggggtatg ggcgggtggc attgaaacac cttcggtgga aaccccatcg gaaggctcaa
     3061 aattttttgg ttttgatatg gataacgaat tcatcagtgg ttttgatgta ggagcatggg
     3121 gaatattact ctgatggcga aaaatgactt taaagcgttt gcaacgggta aaaatgccaa
     3181 tgttatgtcg caggaggaat gggaagcgtt gcctgcgctt ttatccggat ttacagcagg
     3241 gaaagcatcc agtgcgcaag tcaataaggt tattcggcag gccagcttta ttgctgcagc
     3301 tctggcccag tttgtaagtg acaaaacgca acgggatgtg cttgataatg gtgatctgcc
     3361 cggttttgtt gaattgctgg gatcggggtt tgctgttgaa tacctgagcc gcaagaatcc
     3421 gtttggcgat atcaaatcga atggcacggt gaaaacggct ctcgaaaacc ttggtttaca
     3481 ggaaacggta aacaaggctg gtaacgctgt ccaaaagaca ggcgatacct tgtccggtgg
     3541 acttactttt gaaaacgact caatcctcgc ctggattcgg aatactgact gggcaaagat
     3601 tggttttaaa aatgatgccg acagcgatac tgattcatac atgtggtttg aaacaggtga
     3661 caacggcaat gagtatttca aatggagaca ccgcctcgct ggcggccagc tcaaagagct
     3721 gatgaatctt aaatgggatt cactaaatat tctggttaat gccgtcatta atggttgcct
     3781 tggcattggt acgacgaatg cgttaggtgg aaattcaatt gctttcgggg ataacgatac
     3841 cggcctgaaa cagaacggcg atggtctgct ggatgtttat gcgaatggac agcatgtatt
     3901 tcgttttcag aatggcgtgg ctattgcttt taagaatatt caggccggaa ctgccagaaa
     3961 attcacgtta tccagcgcca acaactccac gaaaaatgca gcgttttatt tgtggggtaa
     4021 tccatccagg cctgttgttg cagagcttgg tgatgattca ggctggcatt ttttcagcca
     4081 gagaaacccg gataacagca tagtgttcac tgttaacgga caggtaattc cgttaaatta
     4141 cggaaacttc gatgcccgct ataaatatcg aacagagggg gtacaggatg tacggtatgg
//
LOCUS       DADKXM010000220         4171 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.223, whole genome shotgun sequence.
ACCESSION   DADKXM010000220 DADKXM010000000
VERSION     DADKXM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.223"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            244..1344
                     /gene="ompC"
                     /locus_tag="JQR05_003898"
     CDS             244..1344
                     /gene="ompC"
                     /locus_tag="JQR05_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY5325884.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1508..1993
                     /locus_tag="JQR05_003899"
     CDS             1508..1993
                     /locus_tag="JQR05_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY5325885.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWDNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            2103..3434
                     /locus_tag="JQR05_003900"
     CDS             2103..3434
                     /locus_tag="JQR05_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY5325886.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            3758..>4171
                     /locus_tag="JQR05_003901"
     CDS             3758..>4171
                     /locus_tag="JQR05_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HAY5325887.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTINLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLC"
BASE COUNT         1342 a          841 c          708 g         1280 t
ORIGIN      
        1 tacggcccgg cagttaatca gataaattcc gacaatatta aaaaataaat ttcaaatacc
       61 agtaattcac cttatatctc atttcgactt cgatcacata tcgataaata atattatttc
      121 atctattttt ctcattgttt ttaatcacca ttctatttta aaaacagtat gtcatttata
      181 aatgacgccg tttaaaattc gtttagaaaa tacgtttaac gataattaaa aggattctgt
      241 taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc gaacgtagca
      301 cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg caaggttacc
      361 gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta tgcccgtctc
      421 ggctttaaag gagaaacgca aatcaatgat caaatgattg gttttggtca ctgggaatat
      481 gattttaaag gctataacga tgaagccaac ggctcgcgcg gcaacaagac ccgtctggcc
      541 tatgctggtt taaaaattag tgaatttggc tctctggact atggtcgtaa ctacggtgtc
      601 ggctacgaca ttggttcatg gaccgatatg ttgccagaat ttggtggcga tacctggagt
      661 cagaaagatg tcttcatgac ataccgtacc accggtctag caacctatcg caactacgat
      721 ttctttggct taattgaagg gctgaacttt gccgcgcaat atcaaggcaa aaatgaacgt
      781 actgacaaca gtcatcttta tggtgctgac tacacgcgtg ccaacggtga cggtttcggt
      841 atctcctcaa cttatgttta tgatggcttt ggtatcggtg cggtatatac caaatccgat
      901 cggacaaatg cgcaggaaag agccgctgct aatcctctca atgcctccgg taagaatgca
      961 gaactatggg ccacaggtat aaagtatgat gccaacaaca tctactttgc agctaattac
     1021 gctgaaacat taaacatgac cacctatggg gatggttata tctctaacaa agcacaaagt
     1081 tttgaagtgg tggcgcaata tcaattcgac ttcggcttgc gcccatcact cgcttacctg
     1141 aaatcgaaag gcagagatct gggccgctac ggcgaccagg acatgattga gtatatcgac
     1201 gttggtgcga cgtatttctt caacaaaaat atgtcgacct atgttgatta taaaatcaac
     1261 ctgattgatg aaagcgactt tacccgtgcc gtagatattc gcaccgataa catcgtcgct
     1321 acgggcatta cctatcagtt ctaacgtcct ctcctgacca gcccttctct gggctggttt
     1381 ttgatttaag tgctgctgta tgactcctta agtaaaaatg agcaattttt gatatttctg
     1441 gtcattcacc tcaattaata ttattccata tcctccgggg acatcttact ataatattta
     1501 ttgatgagtg attatgacca atattaatac agcttgtgta aaaaataatg ccagttatca
     1561 acttaataac gcattaccta acaaagaaac catctccagc aatttttgtg aacgactaga
     1621 acaatgggat aataaatcgg ttaataatgg tgaagaaaga gcaattgccg tggagcgaat
     1681 taaagaagct tacaattcga acatggcatc tcttgatttg tcatatcttg atttgagtga
     1741 attaccgcct atcccctcga cagttaatac gttaaatctg gaaaacaatt gtctaacttg
     1801 tcttgactta gctgacaatg ccagtctcgt taatatcaac ctcagcgtta ataaaattaa
     1861 cacgataacc tttccaaaag aaccaaaact ggaaaacatt tatattgatc ataacaatct
     1921 ggaaagttta gatttaaaaa atcagcattc attggttaat ctggaagcgc aaaataatag
     1981 cctaaaaaaa tgatatttct gatagttata aactgaaatt tcttaatctt gattataaca
     2041 agctagcatc gctggatgtc tcccggcaag aatctctgat tgagttaagt gcccatcaca
     2101 atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc actttaaacg
     2161 acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat ttaaacttaa
     2221 gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca aaacatcttt
     2281 ggcatgtatt agttaacggc atcaacaatg atccacttgc acaaatgcag tactggactg
     2341 cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca ggacttaatt
     2401 taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac
     2461 tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg aatagtaatg
     2521 ctcttgagtc aataaatttt cctcaaggca gaaatgtaag tattacacat atatctatga
     2581 ataataatgc tctcagaaat attgatatag ataggctttc atcagttact tattttagtg
     2641 cggcacataa tcaactagag tttgtgcaat tagaatcttg cgaatggctg caatacctga
     2701 atctcagcca taatcaatta actgatattg ttgcaggtaa taaaaatgaa ctcttactgc
     2761 tggatctatc ccataataaa ctaacaagtt tacacaatgt cttatttccc aacttgaata
     2821 cgttacttat taacaacaat ttgctttctg aaattaaaat attctatagc aacttccgca
     2881 atgttcagac attaaacgct gctaacaacc agttgaaata tataaatctt gatttcctga
     2941 cttatcttcc atctatcaaa agtttaagac tggacaataa taaaataacc cacattgata
     3001 ctaataatac atccgatatt ggaactttat tccccataat aaaacagagc aaaaacttaa
     3061 attttttaaa tgtttctggg aagaacaatt gccctactat gcagctcatg ttatttaatt
     3121 tgttttcccc agcacttaag cttaatactg gcctggcaat tctttcgcct ggtgcatttg
     3181 aagttcactc tgacggaata gatgtggata acgaattgtt tcactatact attaaaaaag
     3241 catatacccc atataatata cacacttaca agacagagga agttgtaaac cagaggaata
     3301 taaaagttaa aaacatgacc ttagatgaaa taaacaatac ttactataat aacgattatt
     3361 acaatcaggc aataagagag gaaccgatag actttctgga cagatcgttt tcctccaatt
     3421 catggccttt ttagtgaata aatcatcact aattctataa gtgtctggtc ttgttgagaa
     3481 tatagttaat atgtaaccct ttagattcac gatctgaagg gttgaaacat gacatacgtt
     3541 caaagattaa cccccgtttg aaggttagct tgttagttac atttagtaac acatatctgg
     3601 ctacttaatc cccattctgc ccccgcggtt acgctttctt atctattctt tcaatcagca
     3661 cttcacccaa ttcatcctta attctaataa ctggctattg cgccttacct cctgttttgt
     3721 cttttttcat taccaatgtg tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa
     3781 tagtcgttat cttttacgcg actggaaacc agaaaatccg gccttctggg aaaataaagg
     3841 aaaacatatt gctcgaagaa acctctggat atcagtcagt tgtctacttc ttgccttctg
     3901 tgtctggatg ctatttagcg cagttaccat taatctcaat aaaatcggtt ttaattttac
     3961 taccgatcaa ctctttttat taaccgcatt accctccgtt tctggcgcat tattgcgtgt
     4021 tccctactcc tttatggtgc ctatattcgg tggacgccga tggacggttt ttagtactgc
     4081 aatcctgatt attccttgcg tctggctcgg aattgccgtg caaaatccga atactccttt
     4141 tgggatattt atcgttatcg ctttgctatg c
//
LOCUS       DADKXM010000221         4123 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.224, whole genome shotgun sequence.
ACCESSION   DADKXM010000221 DADKXM010000000
VERSION     DADKXM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.224"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            81..353
                     /locus_tag="JQR05_003902"
     CDS             81..353
                     /locus_tag="JQR05_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325888.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSSLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
     gene            complement(319..663)
                     /locus_tag="JQR05_003903"
     CDS             complement(319..663)
                     /locus_tag="JQR05_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY5325889.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(668..883)
                     /locus_tag="JQR05_003904"
     CDS             complement(668..883)
                     /locus_tag="JQR05_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY5325890.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1033..1194)
                     /locus_tag="JQR05_003905"
     CDS             complement(1033..1194)
                     /locus_tag="JQR05_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325891.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(1191..1394)
                     /locus_tag="JQR05_003906"
     CDS             complement(1191..1394)
                     /locus_tag="JQR05_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY5325892.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            1639..1974
                     /gene="iraM"
                     /locus_tag="JQR05_003907"
     CDS             1639..1974
                     /gene="iraM"
                     /locus_tag="JQR05_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY5325893.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(2255..2386)
                     /locus_tag="JQR05_003908"
     CDS             complement(2255..2386)
                     /locus_tag="JQR05_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY5325894.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2886..2962)
                     /locus_tag="JQR05_003909"
     tRNA            complement(2886..2962)
                     /locus_tag="JQR05_003909"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2926..2928),aa:Arg,seq:tct)
     gene            complement(3063..3138)
                     /locus_tag="JQR05_003910"
     tRNA            complement(3063..3138)
                     /locus_tag="JQR05_003910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3103..3105),aa:Ile,seq:cat)
     gene            complement(3282..4103)
                     /locus_tag="JQR05_003911"
     CDS             complement(3282..4103)
                     /locus_tag="JQR05_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY5325895.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1168 a         1099 c          771 g         1085 t
ORIGIN      
        1 tctgaaccct ggatatgttt atctgtccca gcctgttgaa tcaggctggg aaaaggtaaa
       61 gacaatcaag aggattattt atggacaata acaccatttc tctacaggag ttgctcgact
      121 gcatttccag gcttcgggat gatgtaaatg ccctcactgt tgcattttca tctctggcct
      181 tatcaattcc cagagaacaa atgcaaccaa cactggcatc gctcctgctt gaatcacgca
      241 accccaaatg gtcccaggaa caacaaaatt ctttcaagtg gctggcggca ttactggaag
      301 aaaaatatgc tggtaaaatt accatttcgg tggagtcttc agagaatcag taattcttcc
      361 tggtagcttt cctttgtagg ttatccacac attctgcgcc tctaaaatta tggggcgctt
      421 ttccggcgac tgctcatccc cttcacataa cccggcagca acatccagga agacctgtct
      481 gatgctcatt ctggctgctg cctcataaaa ctccagcgcg gcaccttcaa cacggtccag
      541 cgagatgtcc aggtcaaaaa tttcaccgtc aaagcgtttt ttgtcccgta acgctaaagt
      601 taccgtaact ttattctcaa aattgcggat ccctttcaca atcagttcat agttttgagt
      661 cattgaatta ctctccccgt gcagccttac gacggtcctc tctgattttg aaatacaggt
      721 tagtcagata tgtcagcagc ccaaacagca gactccccag cacgcctatt gccgcccact
      781 gagacgggga aaccctgtcc agcaactgca ggaaccagta gcccgttccc accgctgacg
      841 tggtgtatga cacacctgtt gtgatttttt ccatctggtc cataccccgt ctcccgttat
      901 ccggaagctg acaacaataa aaaagccacc agttaactac tgatggctct gataactcat
      961 gcaagcgtct cagacgaccc actgacacta ccggtgagtt taacgatacc ttccatttga
     1021 ctggctcact ttttatgatg atgccggtgc atttatctcc agcaccagac tttctatctc
     1081 aacgccatac gttgcatttt tggtaatatc cgtcagcgtc agtgcattta gtcccactgc
     1141 cagactgtct tttatggcct ggaatgccgg gccagtacga tgacgtagta tcactccggc
     1201 tcagttgcac cactgaccac cacatcacct tctgctgcaa tcgcctgcat cagggtataa
     1261 ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt atgtgtcagc
     1321 ttttccgcaa gatctgacgg cggcgccgcc ctgacaacat catagtgttt aaatgccatg
     1381 gttctttcca ccatctgaaa aataattctt taaaatacct gacatgtaat acagaaaaaa
     1441 cacaaaacca taccttaaat aaaaacctga ttatcaagca gatatgcatg gataaactac
     1501 aagacgagat ataaaccacc ctgcatttaa ataaacaata aacaacatca gaaaaataat
     1561 tctgctctat ggtttaattc aaaaatatca tttatacttt tcagaacatc accagcaagg
     1621 cataaacaag gaaactaaat gaagtggatt gtgattgata cagttatcca gccatcatgc
     1681 ggaatatctt tttcagtcat atggagtaaa ataaaattaa taatctggta tcaatcggat
     1741 gctttcttac ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac
     1801 aaagtgctac ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattta
     1861 atcaaaaaca gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat
     1921 aaccgttgca ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt
     1981 tactcacatc tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg
     2041 ctatgtgcca ttgcatggtg ccgggtgcct cccggtgaat tcagtaccag cacctgaatc
     2101 cgcgattatc ccatatacct actcgctgat tgcccctccg cacaggggga ttcaccatgc
     2161 cagtttcttt taacaaactc cccgcaaacc agacaacagt caaccgcctg aattgtgaag
     2221 tatttaaaaa tttctcccgc taactgatac ccggctaaca gtctggcgtt ttctttttca
     2281 gcaacgggaa agcaacaacc accacacccg ccaccagcac accgtcagcc ggcactgaca
     2341 ttatccggct gctgcaatgc cattcacaaa aacagtaagc aatcactttt taccgtaaca
     2401 ggtgataatc cagatatgta tctaccccag atgagtaatc cgaagttcat ccataccaca
     2461 ggtcctggct attctgttgt actcctgaac aagagcaaat aattctgaat tagcaaccat
     2521 gaactcatcg caaaccctct gtatagcatc actattcaga ataataacgt ctcttcccga
     2581 aagacgatca ggagtacaga acaaaactgt caaacggctg aaggcctttg ctcgtgctgc
     2641 attgactata tcaatacgct gcctaaggat gaaacacccc gacgcctcat caatattcac
     2701 tctacccaca ccatatgaat gataaatact taatgctgaa aaaaccatta gaccgtataa
     2761 caaatactca atcaatactt aacagaactt ttatttttga caaacatata atattttcaa
     2821 caatatcctg agccaggtat atttcagtat aaggctctgc cggaaggaat ctggaagaat
     2881 gaatatggcg cgctgtactg gattcgaacc agtgaccgat tgcttagaag gcaattgctc
     2941 tgtccggctg agctaacaac gctgaatacc gataatggac cgccatcggg gacccgcccc
     3001 cgcaccaaca accctgttat cgtgtcgtct gctcttcctg ataagctaat ggcggtttgt
     3061 gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
     3121 ccactgagct aaagggccgg gagcagaata ataacggtcc gtaattaatt tcgcaataaa
     3181 aaacccgctc agcggcgggt tgtagaaact cttctaacgt caggcataaa acgcccatca
     3241 ttatgacgaa tttaccacag attccggaaa aatcaacctt gttacctagt tacctttttt
     3301 aactgccgct cagcccatgc ttcttcaata tcaaaccggg tcaccagcgc atcataaaat
     3361 ttcttaactg ttttttccca tgacgcgcgt gttatctggt ttgtcacctc gcatatagca
     3421 ttaaatgcct ccgttgatgg tagtctttca tagccacgac caccacaacg ctggcagtct
     3481 ctgataacag gcataccacg ttttaccgac tcttcacgat gaatggcgac accacgccca
     3541 cggcaatcct tacaggcggt ggaaacctca ccctttccgc cacactccgg acaggcaact
     3601 tttaccacct ccctgacttt tttccattct tcccagtaag acggatacac acctttcgta
     3661 cactttgccc ataccggcgg cttaccatcc ggatactgga tcttatttgt aaaaactatg
     3721 ctttcaataa atttttcccc atagcaacaa gggcactgct ttttactcgc tgcgctgcgg
     3781 gcataatcct caaaagcgta cgaagccata atgcgcatca ctaccggttt tatttctgcc
     3841 ggaagttttc tcaacgccgc cacacgatcg caccgactga gtgcataatc tgccagtaat
     3901 tctgttgccc gcgccctgtc attcatactg atgcccattt tccccaggaa cgcagaaaaa
     3961 cccatctcag cccgattctg tgtcatgccc tgcgcggcca tcacatcagt gatactcagc
     4021 gcatcttttg acgttgaggc cgatgcatcg gtcaggccag gggattttgg ggagtagtat
     4081 ttcggtaaat cttccagttt cattttttga cctgctctta atg
//
LOCUS       DADKXM010000222         4030 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.225, whole genome shotgun sequence.
ACCESSION   DADKXM010000222 DADKXM010000000
VERSION     DADKXM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.225"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(33..1487)
                     /gene="xylB"
                     /locus_tag="JQR05_003912"
     CDS             complement(33..1487)
                     /gene="xylB"
                     /locus_tag="JQR05_003912"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY5325896.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAMVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="JQR05_003913"
     CDS             complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="JQR05_003913"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY5325897.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3247..>4030
                     /gene="xylF"
                     /locus_tag="JQR05_003914"
     CDS             3247..>4030
                     /gene="xylF"
                     /locus_tag="JQR05_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5325898.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTM"
BASE COUNT         1060 a         1064 c          920 g          986 t
ORIGIN      
        1 tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga
       61 tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt
      121 agatgcgact gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc
      181 gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg
      241 taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcgcc
      301 ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg
      361 ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca
      421 tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc
      481 tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct
      541 gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcgaccca atccagacac
      601 gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag
      661 ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc
      721 gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca
      781 gctgcattgt cgccaccgcc tgcgacaact ggcaccatcg ccatacccca cgctttcgca
      841 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
      901 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
      961 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     1021 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     1081 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     1141 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     1201 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     1261 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     1321 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca aagtggatgc
     1381 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     1441 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     1501 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     1561 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat
     1621 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     1681 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     1741 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     1801 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     1861 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     1921 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     1981 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     2041 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     2101 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     2161 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt accctggaag ccgattttat
     2221 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     2281 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     2341 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     2401 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     2461 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     2521 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     2581 ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     2641 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     2701 caaacatgtc cgccccgttc cagcagaagg tgtgccagta gcaggcggca aaacgcaagt
     2761 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     2821 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     2881 tattgaactc cataatcagg taataccgcg ggtgatggat gatgtcgtaa tattgggcac
     2941 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     3001 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     3061 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     3121 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     3181 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     3241 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     3301 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa
     3361 cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg tgcgaaagta
     3421 tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgatt
     3481 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt
     3541 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat
     3601 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     3661 gccttggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     3721 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattct
     3781 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
     3841 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
     3901 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
     3961 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
     4021 caaactatga
//
LOCUS       DADKXM010000223         3997 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.226, whole genome shotgun sequence.
ACCESSION   DADKXM010000223 DADKXM010000000
VERSION     DADKXM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.226"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..67
                     /locus_tag="JQR05_003915"
     CDS             <1..67
                     /locus_tag="JQR05_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5325899.1"
                     /translation="AQQQQLTSWQEQGHDIFPLGI"
     gene            127..234
                     /locus_tag="JQR05_003916"
     CDS             127..234
                     /locus_tag="JQR05_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY5325900.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(215..946)
                     /gene="yehW"
                     /locus_tag="JQR05_003917"
     CDS             complement(215..946)
                     /gene="yehW"
                     /locus_tag="JQR05_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY5325901.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQATLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGFSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(951..1877)
                     /gene="yehX"
                     /locus_tag="JQR05_003918"
     CDS             complement(951..1877)
                     /gene="yehX"
                     /locus_tag="JQR05_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY5325902.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(1870..3027)
                     /gene="yehY"
                     /locus_tag="JQR05_003919"
     CDS             complement(1870..3027)
                     /gene="yehY"
                     /locus_tag="JQR05_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY5325903.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3034..3951)
                     /gene="osmF"
                     /locus_tag="JQR05_003920"
     CDS             complement(3034..3951)
                     /gene="osmF"
                     /locus_tag="JQR05_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY5325904.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
BASE COUNT          969 a         1214 c         1004 g          810 t
ORIGIN      
        1 cgcccaacag cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg
       61 tatttaatga ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag
      121 tgcattatgc gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc
      181 ggaattggtg gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct
      241 gcaccagcct ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc
      301 cctggatcac atacgcggta ttaaatccgc taaacccgat gatgatgggc gtacccagcg
      361 tactggcccc taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc
      421 gcacgcccgc cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac
      481 ctcgactcat tcccatacct ttcgcaactt ctgtcacgct gggatcaatc gctcccagcc
      541 cggcaagtgt cgcctgcagg atgggcagca caccgtaaag gatcaaggcg ataatcgctg
      601 gttgcagacc aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag
      661 tctgtccaac ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag
      721 taacagcaat tccggcacct gtgccaatga tcaccgcaaa caaactcgaa attcccacca
      781 gccagaaatg agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca
      841 gttgtgggaa caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagcgcca
      901 caaaaagagc aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc
      961 accagcagat cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc
     1021 agcacctcgc atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct
     1081 gccagtgcct caccttctgc ccgttcttcg cgacgcacgt aatccgccac gctacgtaac
     1141 gaaagcaggc gcacccccag ttcactacgt ccaaaaaact ggcggacaaa atcattcgcc
     1201 ggacgagtca gcatcgtcag cggattcccc tgctgcacca cttcaccgtg atccatcaat
     1261 accagatgtt ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatagtg
     1321 cgccccagca aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg
     1381 tccagtgcgc caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcacgc
     1441 gccactccca cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgcaaa
     1501 tttgactcca gccccagtag cgccattaat tcgtcgatac gatcgtcaat ccgcgcccgc
     1561 gaccattttt gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgtgggaac
     1621 aggccaatag attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag
     1681 cgaatttctt ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc
     1741 attttcaggg tggtggattt gccggagcct gatgtgccaa tcagcaccga aaaactccct
     1801 tcctgaaaat tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca
     1861 tggctaaatt caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc
     1921 gtcggtaagc accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc
     1981 gctgcttagc agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc
     2041 aatcaccgcc atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg
     2101 taacgccagc ggtaactgaa catgcaggaa acgctgcgcc ccgctcatcc ccatcgctct
     2161 ggcgctctcc agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag
     2221 cggcagcaag gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat
     2281 gccgagcttc cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag
     2341 gccaaagagc gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc
     2401 ctgccgcgca gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg
     2461 cagcaccgca ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg
     2521 acggttggcg tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca
     2581 cagcggaata atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat
     2641 tcggcggatg gcatcgctac aggccagcaa tgccagcgcc gcagccagcc agaaaccact
     2701 gccgaggctg gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc
     2761 agctccccac accaacaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc
     2821 ttttttagcg gggacaaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca
     2881 gatcgtttgc ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg
     2941 cgcgtaactg ataaacggca gcgccgctgc gattgccgtc agcaacaaca gcagcgccag
     3001 aacaggatta atacgtagat aagtcacggg aaattacttc gcccaccctt tttgtttcag
     3061 gtagtcggca gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg
     3121 ctgcaatgtt tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg
     3181 atactccttc aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc
     3241 ttgcggatcg cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc
     3301 cattgcggca ttaacgccag aggtttgctg ggcagcggct ttaatcgtca ccgccgtgtc
     3361 gccaccagcc agtgacagca actgatcctg accgagctta aagccatagg ctttttcaaa
     3421 cgcgggtaac gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc
     3481 gccctcttgc agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac
     3541 atcctgacgc acggcgatag tccaggtgtt atttgcaggc gcgggcgtca gccagattaa
     3601 cttgttgtgc tccgcatcga gctttttgac tttctcgtag ccctgcccgg catttttcca
     3661 cgctgcatcg ttttcatctt taaagaagaa agcgccattg ccggtatatt ccggatagat
     3721 atccagttca ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt
     3781 attcaccgtc ggtacgccgt ggctttcgag tacctgcaag atgatgttgc cgagcagcgc
     3841 gccttcggta tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct
     3901 cacggctgcc aacataacca gtgaacctgc ccagagcttt aagagtggca tgatgctttc
     3961 ctcggtaatg actccaactt actgatagtg ttttatg
//
LOCUS       DADKXM010000224         3987 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.227, whole genome shotgun sequence.
ACCESSION   DADKXM010000224 DADKXM010000000
VERSION     DADKXM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.227"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..321
                     /locus_tag="JQR05_003921"
     CDS             <1..321
                     /locus_tag="JQR05_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325905.1"
                     /translation="QGGGELPLVPGKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVL
                     AILPRELRHRLCIFDTLERRALLAAQEALSTAIDAHDDAVQAVYRKAHFSGGGASDDS
                     VVVH"
     gene            412..1167
                     /locus_tag="JQR05_003922"
     CDS             412..1167
                     /locus_tag="JQR05_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016060759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5325906.1"
                     /translation="MSMKLMAKAMEIKVGNPLRKLVLIKLADNASDQGECWPSYQYIA
                     DQCEISKRSVINHIAALCESGLVKKITRKGEKGNTSNIYLLHLDGAENLPGNSANNSL
                     HCASDASGSAGVAPVASAGVAPRTSHSFEPVNEPDVGGSADADPQVSHRAKNKIDWQR
                     VLDSYHEILPEMPSVKILTDTRKKNLRTFWQKFGFDQQRWESYLRYVAENCRWMLEDR
                     PNGSGGFWKRKNLDYLITERCYVAVKEARANDR"
     gene            1157..2560
                     /locus_tag="JQR05_003923"
     CDS             1157..2560
                     /locus_tag="JQR05_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY5325907.1"
                     /translation="MTDNTFPVPYRVDAEQAVLGCLMVNTDTERAGLVYSMLKPESFY
                     VAAHRVIFREIRGLFRAGKPTDLLSLANVIEAKKLDAETGGFAYLAEISRSATLSAMV
                     HYAGIVREKSILRYAVEKLHACIGIMNQPTDTSATERLGAVQQVIGTVAEYARTGKTG
                     GLRPVSDVVNDWIDDLERRFSSPENAAGLTLGISSLDRLMAPKHVLRGSLVVIGARPK
                     MGKSASLNRIVTHFALNHRLPTLVFTLEMTDRSLVERMVAQEARVNSEIFYVGASDDS
                     DMARAMAKAAELAESNLMIDSTPGVTLAHVIAECRKVKRQRGVVGLVAIDYLTLMKAE
                     AAERRDIAFGDITTGLKNLAKELDCVVLLLTQLNRKLEDRADKRPTPSDSRDTGQIEQ
                     DCDVWIGLYRDAVYNRNADSQLMELILRLNREGATGTAYALMDNGSVTDVSAEDAARR
                     LRLGGPARKPYAQTEDF"
     gene            2727..3488
                     /locus_tag="JQR05_003924"
     CDS             2727..3488
                     /locus_tag="JQR05_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAY5325908.1"
                     /translation="METVFDALKAMGKATSTELAARLDISREEVLNELWELKKAGFVD
                     KSAYTRRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPLFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWECVCAALRELNKHRDIVRQITDISRRVASEK"
     gene            3504..3896
                     /locus_tag="JQR05_003925"
     CDS             3504..3896
                     /locus_tag="JQR05_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY5325909.1"
                     /translation="MAKVFTPEEREEVKARIVELVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYDRRQN
                     IICRECRKSEVMQRVLAFYQGNFQEVMA"
     gene            3893..>3987
                     /locus_tag="JQR05_003926"
     CDS             3893..>3987
                     /locus_tag="JQR05_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY5325910.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKR"
BASE COUNT         1050 a          891 c         1177 g          869 t
ORIGIN      
        1 caggggggcg gcgaattgcc cctggtacca ggcaaggact ggaacaatca gcagaatatc
       61 tatcaccgtt ggttgaaagg tgaaacgaaa gcgcaaaggg aaaaaattca gaaactgatc
      121 cctgcggttc tggcaattct tccgcgcgag ctgcgtcacc gactctgtat cttcgatacc
      181 ctggaacgcc gtgcattact ggcggcgcag gaagcgttga gtacggcaat tgatgcgcat
      241 gatgacgcag tccaggccgt ttaccgtaaa gcgcatttca gcggtggtgg ggcgtccgac
      301 gattctgtcg ttgtgcattg atgtttatgc cgaacctcct ctggttctgt tgattgggga
      361 atcacagatt atatccggag gaaggttcgg caccagacga ggtttctata tatgagcatg
      421 aaactaatgg caaaagcaat ggaaattaaa gtgggtaatc cattgcgtaa gctggttctg
      481 attaagctgg ctgataatgc cagcgatcag ggcgagtgct ggcccagcta ccagtatatc
      541 gctgaccagt gcgagattag caagcgttct gtgattaatc atattgcggc gctttgtgag
      601 tccgggctgg taaaaaaaat cacccggaaa ggtgaaaaag gtaatacaag taatatctat
      661 ctccttcatc tggatggtgc agaaaattta ccggggaata gtgcaaataa ttcgctacat
      721 tgtgcatcag atgcatcggg tagcgcagga gttgcacctg ttgccagtgc aggagttgca
      781 cccagaacca gtcactcttt tgaaccagtc aatgaacctg atgttggtgg atctgctgac
      841 gcagatccac aggtcagtca tcgcgcaaaa aacaaaatcg actggcagag ggttctggac
      901 agctatcacg aaatcctgcc tgaaatgcca tcagtgaaaa ttctgacgga taccaggaaa
      961 aaaaatctgc ggacgttctg gcagaaattc ggttttgacc agcagcgatg ggagtcctat
     1021 ctgcgatacg ttgctgagaa ctgtcgctgg atgctggaag atcgtcccaa tggcagcggt
     1081 gggttctgga agcgtaaaaa cctggattac ctgatcaccg aacggtgcta tgtcgctgtc
     1141 aaagaggcgc gggcaaatga cagataacac ctttccggtg ccataccggg tcgatgccga
     1201 acaggcagtg ctggggtgcc tgatggtaaa caccgacact gagcgtgcgg ggctggtgta
     1261 ttcgatgctg aaaccggagt cgttttacgt tgcggcgcac agggttattt tccgtgaaat
     1321 tcgcggactt ttccgtgccg gaaagcctac ggacctgctc agccttgcga acgtgatcga
     1381 ggcgaaaaaa ctggatgctg agactggtgg ctttgcgtac cttgctgaaa tcagcaggag
     1441 tgccacgcta tcggcgatgg ttcactacgc cgggattgtg cgggaaaaat ccatcctgcg
     1501 gtacgcggtg gaaaaattac atgcgtgcat cgggatcatg aatcagccga cagacacgag
     1561 tgcgacagaa cgacttggcg cagttcaaca ggtcattggc acggtggcag agtatgccag
     1621 aacaggaaaa acaggcggtc tgcgcccggt aagtgatgtg gttaacgact ggattgatga
     1681 tcttgagcgt cgcttttcca gcccggagaa cgcagcgggt ctgacgctgg ggattagctc
     1741 tctggatcgg cttatggccc ccaaacatgt gctacgtggt tcgctggtag tgattggtgc
     1801 aaggccgaaa atgggtaaaa gcgcctcgct gaacaggatt gtgacgcatt ttgctctgaa
     1861 tcatcgtctg cccacactgg tattcaccct ggaaatgaca gatcgcagcc tggttgagcg
     1921 catggttgcg caggaggcga gggtaaattc tgaaattttt tatgtcggtg ccagtgatga
     1981 ctcggatatg gcgcgggcga tggcgaaagc cgcagaactg gcagaatcaa acctgatgat
     2041 tgacagcaca ccaggggtaa cgctggccca tgtgatcgct gaatgccgga aggttaagcg
     2101 tcagcgtggc gttgttggcc ttgtggcgat cgattacctg acgctgatga aggcggaagc
     2161 cgcggagcgt cgtgatattg ctttcgggga tatcacaacg ggcttaaaaa atctcgctaa
     2221 agagctggac tgcgtggttt tacttctgac gcaacttaac cgaaaactcg aggacagggc
     2281 agataaacgc cccacaccaa gcgacagtcg cgacaccggg cagattgaac aggattgcga
     2341 tgtgtggatt gggttgtacc gggatgctgt ctacaaccgt aacgcagact ctcagcttat
     2401 ggagctgatt ctccgtctga acagggaggg agcaacagga acagcatacg ccctgatgga
     2461 taacggttca gtgactgatg tcagcgcaga ggatgctgcc agacgtctga gacttggtgg
     2521 tccggcacgt aaaccctacg cacagacgga ggatttctga tgcagtcact ttgtgatgtt
     2581 ctggaaaaaa cgctgcttca gttcgggcgg ggggaaacgg cggagcagaa aacacgaaac
     2641 cagaagaatc tggttcatct gagaaaaatg attgatgcag ccaggctggc tgaaaagctg
     2701 gaaaaacaaa accggaggta attttaatgg aaactgtttt tgacgcactg aaagcaatgg
     2761 gaaaagccac atccacagaa cttgctgcgc gacttgatat cagtcgtgaa gaagtgctga
     2821 acgaactatg ggaactgaaa aaggctggtt ttgttgataa aagcgcgtac acccggcgtg
     2881 tggctgataa caacgttcag caggaacagc cagcgcaggc agaactgccg gaagaaacca
     2941 ccacagcaac agtagcgaaa atctcagagt gcgatttaac cgcgacgatt gaacaacgcg
     3001 gaccacaaac ggctgatgag ctggctacat tgtttggtac cacatcacgc aaagtggctt
     3061 caacgctggc aatggcaatc agcaaaggtc gtctgattcg cgtaaatcag ggcggtaaat
     3121 ttcgttactg cataccgggc gataatttac cagcagagcc gaaagcagca tcggtagcgg
     3181 aaactgatgg taaggccttt cctcagcccg caggtgttgc attaccagta caggaggctg
     3241 caacacagga agatattaaa acagaaacgg tggcggacat tgtgcagtcg ctgccattgt
     3301 tcaccgaaac gcgagcggat gacctggttt taccatcgct gcatatggca aaccgcgaac
     3361 tgcgtcgggc gaaaagtcat gtccagaagt gggagtgtgt ctgcgccgcg ctgcgggagc
     3421 tgaacaagca ccgggatatt gttcgacaga ttactgatat ttctcgccgt gttgcatcgg
     3481 aaaagtgatt gccggaggca cctatggcaa aagtatttac accagaagag cgggaagaag
     3541 tgaaggcgcg cattgtggaa ctcgtgcgcc tgagcggacg agaaactttt cgacaactgg
     3601 cagataaaac gggtgtcagt aagaccgcta ttcgtcgttt atctggtgcg cttgcggcca
     3661 gtggtgatgt ctggctctct gattgcgggg tatttccatc agagcaggcg tatcgcgtat
     3721 ggcgtaagac accggagaag gctgctgacc cgacactgat tcgaaagtta cctgacggag
     3781 aaatacgccg ctacgacagg cgtcagaaca taatctgtcg cgagtgccgg aagagtgagg
     3841 ttatgcagcg agtgctggcg ttttatcagg gtaattttca ggaggtgatg gcgtgagggt
     3901 gagagtctat atcgccggtc caatgaccgg gtataaaaat ttcaaccgtg aggcgtttca
     3961 caatgcggaa gaggaactga aacggga
//
LOCUS       DADKXM010000225         3886 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.228, whole genome shotgun sequence.
ACCESSION   DADKXM010000225 DADKXM010000000
VERSION     DADKXM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.228"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..1514)
                     /locus_tag="JQR05_003927"
                     /pseudo
     CDS             complement(1..1514)
                     /locus_tag="JQR05_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            complement(1776..3366)
                     /locus_tag="JQR05_003928"
                     /pseudo
     CDS             complement(1776..3366)
                     /locus_tag="JQR05_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            3751..3886
                     /locus_tag="JQR05_003929"
                     /pseudo
     CDS             3751..3886
                     /locus_tag="JQR05_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
BASE COUNT         1206 a          714 c          639 g         1327 t
ORIGIN      
        1 taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca cgggcgttaa
       61 gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg ttatgtgcaa
      121 caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt ttaggtctgt
      181 aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc cggctagttt
      241 taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc gctaattcga
      301 ccggatcgag agcattggtc atttcatcaa tagatgacag ttatcggtcc tataatgtaa
      361 cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt gtatttgggg
      421 tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc aagtggtttc
      481 atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt tccagctaat
      541 acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat aaattgatcg
      601 ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca tggcgtgact
      661 gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc tcttctgttc
      721 agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt ctcccttagc
      781 aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt ttctccttca
      841 gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc gactgagcca
      901 tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga acaattggag
      961 aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat atcaaataca
     1021 ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac tgcaacatca
     1081 ttagcattat catttactgt catattatta agtaaataaa caatgctgca ccaattaata
     1141 tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat aaactgataa
     1201 cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata tttttctgaa
     1261 tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg gaaacgttct
     1321 atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctagc aatacttgat
     1381 atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa gttggtaaca
     1441 tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt gcctgccaca
     1501 gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata gttaacattt
     1561 tatcttatga catacacctc atattcgatg ggaaaataat tatattttgc acaaatcatt
     1621 cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc ctaaagattg
     1681 tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga aatactggca
     1741 aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa acaactaagc
     1801 cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccagga atttagcccc
     1861 atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg gccaaatttc
     1921 tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt tggtctgaaa
     1981 gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc attgcttcta
     2041 attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaaa tatatttgct
     2101 aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa agcaccgaga
     2161 caataacatt gattttcttc atttcttatt attccgtgat gactggttaa aactttagtt
     2221 tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc accggcagag
     2281 agtttataat tttctagtcg tacagtatag ttaccattaa aattgatttc atgatttcca
     2341 gccagaacga tgacattttt attaatacgg ctatctttat taccctccat atttcgcata
     2401 gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct atcgcctgta
     2461 tgatccccaa ggaagataca aggggttatc gcatcgtcct ttaaaaaaac atgttgatgc
     2521 aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt attattttta
     2581 tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa taaactccaa
     2641 cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac agcgcctact
     2701 cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat cacaccacat
     2761 tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa cttatcctta
     2821 ggataacgtc tctttagaga catcccaacg ccagtcagct ttaaaattac cgctaagaag
     2881 agtaaagaaa ttttcaggta tgatagaaag cccattaact ttataaatta tgtctgattt
     2941 atctccggag aaattaataa aataataacc atccttacac tcaatcttaa ctgtaatggg
     3001 agacgataat tttatcacat ggttattgtc tggtataatt gtaaagataa acgtgttagt
     3061 agtattatcg gcaaaactat tatattcttg aatatctata gatggctcac tattgtaaaa
     3121 ccatattttc tccatctgaa gactatgttc atttaccctc cagtgaccat gttgcaggaa
     3181 atagatgata tgcatgccaa aatcgttatc tatttgcgaa tcattatcca gtttatcaat
     3241 ttcaactatc tttgaacgat gtgttttatc aaccttgcta tggtgttgat gattattaac
     3301 tttatctgaa tgcttatctt tatggtttga agggatgtac ttaaatatat tgtcaattgc
     3361 tttcataaat aacccgcgat atttttgacc tgttagcaac aaataaatga tacatgtctg
     3421 aaagacatat ttacgacgat ataaaacaat aatacttata tgttcgctga ttttaagcac
     3481 cgagactatc cggtctttta ctaaataatg gaaaacaatc catccctgga agacaattgt
     3541 aaaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     3601 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatgcatttg
     3661 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     3721 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     3781 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     3841 tcagggcaac gtcgcgggtc atgtgctgat ttctctggcg gccata
//
LOCUS       DADKXM010000226         3860 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.229, whole genome shotgun sequence.
ACCESSION   DADKXM010000226 DADKXM010000000
VERSION     DADKXM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.229"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..508
                     /locus_tag="JQR05_003930"
     CDS             <1..508
                     /locus_tag="JQR05_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY5325911.1"
                     /translation="ISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDNHTFSS
                     NESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCAHVNHV
                     EALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMRFAQLS
                     TVKSFSPN"
     gene            530..1096
                     /gene="yieF"
                     /locus_tag="JQR05_003931"
     CDS             530..1096
                     /gene="yieF"
                     /locus_tag="JQR05_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY5325912.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMDVIGGACCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1150..2487)
                     /locus_tag="JQR05_003932"
     CDS             complement(1150..2487)
                     /locus_tag="JQR05_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5325913.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2652..3317
                     /gene="yieH"
                     /locus_tag="JQR05_003933"
     CDS             2652..3317
                     /gene="yieH"
                     /locus_tag="JQR05_003933"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY5325914.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3384..3857
                     /locus_tag="JQR05_003934"
     CDS             3384..3857
                     /locus_tag="JQR05_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY5325915.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1030 a          965 c          972 g          893 t
ORIGIN      
        1 tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct
       61 cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca
      121 caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg
      181 ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga
      241 cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa
      301 tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc
      361 cgcgattgaa aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc
      421 ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc
      481 taccgtgaag tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa
      541 attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc
      601 acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc
      661 cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga
      721 agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa
      781 ctactcggta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca
      841 accgctggcc ggtaaaccgg tgttgattca gaccagctca atggacgtga ttggcggcgc
      901 gtgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa
      961 gccggaattt atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat
     1021 tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca
     1081 gcgagttaag atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg
     1141 ctatcgattt taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat
     1201 gattacgcac gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga
     1261 gataaagccc agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt
     1321 aataaacgcc ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt
     1381 catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca ccattcccgc
     1441 cagcggcgac agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc
     1501 ggtacgaccg ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt
     1561 accgataaac gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg
     1621 cgggaatttc cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc
     1681 ggaggagtca aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt
     1741 aaacgacccg gctaaatcta catgacccac aactgtcatt acgctcggcg gtgcagaaac
     1801 gatgccattg tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga
     1861 aaccagcacc gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag
     1921 gatacccaga agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgagtt
     1981 agcgacaatc acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct
     2041 ggtaataccc acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa
     2101 aatggtcaaa agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca
     2161 cggcagcccc attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg
     2221 agccagcgcc accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat
     2281 cagacaggtt gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg
     2341 gttaacaaat acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt
     2401 tcgtgccgta gtaccatgtt cgcgcagttt aaaaacgcgt tccagcatcc tctgaccaga
     2461 agtctgggtg gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc
     2521 gctatcgtat accaaaatgc gacaataggt gcgtttgtga gagacttttt tattggattt
     2581 acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa
     2641 aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg
     2701 acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc
     2761 tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata
     2821 ttgtttccct tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg
     2881 cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct ggagcgctcc
     2941 tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc
     3001 attctatggg caagctgaat atgttgcact acttcccgga taaactgttc agcggctacg
     3061 atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg
     3121 taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa tctggtatcg
     3181 acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg atcgttcacc
     3241 cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa gcgcgtggtt
     3301 gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa ttggcgcaca
     3361 aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat cacggacttt
     3421 attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac atattcttcg
     3481 caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt ctcttattgt
     3541 cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg
     3601 aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt ggcgtcgtta
     3661 tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc tcggagttgt
     3721 ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca ttgctggcag
     3781 gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca tgtcctgaaa
     3841 cagactcttt aagttaagcg
//
LOCUS       DADKXM010000227         3800 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.230, whole genome shotgun sequence.
ACCESSION   DADKXM010000227 DADKXM010000000
VERSION     DADKXM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.230"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            817..1185
                     /locus_tag="JQR05_003935"
                     /pseudo
     CDS             817..1185
                     /locus_tag="JQR05_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1616..2725
                     /gene="fepE"
                     /locus_tag="JQR05_003936"
     CDS             1616..2725
                     /gene="fepE"
                     /locus_tag="JQR05_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY5325916.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2970..3104)
                     /locus_tag="JQR05_003937"
     CDS             complement(2970..3104)
                     /locus_tag="JQR05_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY5325917.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(3129..3563)
                     /locus_tag="JQR05_003938"
                     /pseudo
     CDS             complement(3129..3563)
                     /locus_tag="JQR05_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
BASE COUNT         1017 a          782 c          905 g         1096 t
ORIGIN      
        1 gtcatcgggc attatctaaa cataaaacac tatcagtaag ttggattcat taccctacct
       61 acctcttggc aggaaaaagc gctcgcaaaa acggtgagaa aaaaacaagc ctatatcaga
      121 taacagccct ctcctagaca gggctgttac tttgccgtaa ccacagaatc tgtcatacta
      181 cactaccgcc taagtcagta tacactccgc tagcgctacg tgactggttt agggctgcgt
      241 cccgaaaccc gctaaccacc tctcagctac tggatatcac cccatgccat ctgaacatga
      301 tgaacaactg gattcgactg atacgcagct tcccggcgaa tatgaccttt ttgatttaat
      361 gatgcaggtt actgaagaga acctgcacgc cccggtggat tttgggccgc ctgtcggccg
      421 ggaagcgtta tagctttttc gcttacccgc gtacgtggta atacgccggt attacatctt
      481 atttctttat tttcatgtaa ttacaggtgg ttgtaatacg ttgcgtatta cataaataaa
      541 cggggacttc agtttttacc ttttaattca tgcaattgcg atgaaaatcg cggttgcgta
      601 tggtgtatga ccatttaagg gataatgtaa cctgactttt aaagaatgaa tctatctttg
      661 gctgcgcatg agatcgataa aaagcgttcc acatcaggcg gttcatggat acatgaaccg
      721 gaaaggacaa caataagata attgtaagac attttttgcg tttcaatctt atttttggac
      781 tattatgaga caaatttaag acattatggg cggtatatgg cgaattttaa ccctggtcca
      841 agaacagcca ttgcggctgt cagggattcc aagaacgata tagaaacctg gaagaaaaat
      901 ggtatccgta gggaagagat tgtgactgtg ctgaatgaac gttacggcct gaatatctca
      961 ctggttgcgt ttgataaggc tctttacaga ttgagaaaaa gtacagatga accgtctctt
     1021 gctgatgtgg attctgtgtc tgctgcagaa acgaatttct ctgaagccag tgggcctgac
     1081 aatggttttt ttctgtccgg gaatgacttc atgtgagcag aagaagaatc tcaaagagtg
     1141 gttgagtcat tcagagatag ccttagcagg aagaaaggat tctgattttg aattctaagt
     1201 agtgaaatag tggatcctct actcgaaatt gtcggatacc tgcaattacc taccgtgcct
     1261 tacggcttgg tcaatgcctc actgccacgc aataaattgc gtccccgttc accgttttcg
     1321 gatgggaaga atgccggaaa caggacttac cctggctgaa tggggtgaat tttcgcgcta
     1381 tgcacttgcg tgcatactca tgcatgcagt aaaaacagag cctgcgcgtt tctggcgggt
     1441 tttcgggtgg tttgttgcct gttttgccgg tttcccgtca gaaatgccct gagagcctct
     1501 gaggcggtgc acgcaataaa atgttatgtt aaatgtctgc tgaatggttt ttaggttttt
     1561 tcctaacata tgatagaata aaaaaaccat atgttaatat attgtgtttt tttcaatgaa
     1621 aatctcaaag gtctctgacg aacatttttc taattacaac atgttgccga atggtaatga
     1681 agaaattgac ctttttggct tgattagcgt tttatgggca gcaaaaataa aaattgttgg
     1741 ggtgattcta ttttttgctt gtattggctt tcttgtttct tttgttttgc cacaaaaatg
     1801 gactagttct gctgtggtta ctcctgctga gtctgttcaa tggagtgaat taaataagga
     1861 gctttccaag cttcatgttc tgggagtgga ttttgatatc aatcgtgata gcgcatttgc
     1921 tctgtttatt aagaaatttc agtccgttaa ttcgttgaat gagtatatga gatcatctcc
     1981 ttatgttatg gagattatta aaaagaataa cattagcgct ctcgaactgc atcgtgcaat
     2041 tgttgagttg agtaaaaata tgaagtcgat tgatgataat gccagtaaga aaaacgacaa
     2101 gtcatcattg tatgtatcat ggactctgag ttttactgct ccaacaagta aagaagctca
     2161 cgatgtgttg tctgggtata ttaattatgt ttcttccctt gttgtaaggg atttgatgga
     2221 agatataaga aataaactag aagttaaaac taatgttgaa aaagaaattc ttgcactgga
     2281 tgagataaaa attagaaacc agctgaatgc agatattcga cgcctcaatt attcactgga
     2341 ggttgctaat gcggctggaa taaaaaaacc tgtatacagc aatggtcaga ttatgaagga
     2401 tgacccagat tttcctgtgg ctctcggttc tgatggtata gcaactaaat tgaacatcaa
     2461 aaaatcaatc aaggatgttt cggaattgag tggggagttg cgaaatcgtc aatatgttgt
     2521 gaatcaattg gttgtggcga aagtggggga tgttgatttt atgccgtttc agtatcaatt
     2581 gagcccaacg ttaccggtca gaaaggatgg gccaagtaat gccataattg taattctttc
     2641 ttctgtcatt ggagggatgc ttgcatgtgg ctatatactt ttacgtcatg ccatggaggc
     2701 cagaaagatg agtgtgattg agtaaaagtt gtgctgatgc atcattttat aagcaacttt
     2761 tataaaaagt tcaaaggatc ttcttgagat cattccctta cagccgtagt cttctactct
     2821 agaaaacgaa aaaaccacct ggggaggtgg tttttcgaag gttcagtaag ttggggaact
     2881 tctgaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatctgtcct tctagtgtag
     2941 cctcagtaag gctgccactt caagaactct caatacatct ctcgcacatc ctgtttacca
     3001 gtggccgctg ccaatggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3121 cgaactgcct acccggaact gagtgtcagg cgtggaatga gacgaacgcg gccataacag
     3181 cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg
     3241 gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3301 tttttgtgat gcttgtcagg ggggcggagc ctatggaaaa acgccaccgg cgcggccttt
     3361 tttcgggtcc tcttttttgc tcacatgttt tttccggtgt tatcccctga ttctgtggat
     3421 aaccgtatat ccgtctttgc gtgagcagac accgctcgcc gcagccgaac gaccgagcgc
     3481 agcgagtcag tgagcgagga agcggaagag cgccggaagc tgcattttct ccttacgtat
     3541 ctgtgcagca tttcacacct catatgtcgc tgttctctca ccatctgctc tgccgccgca
     3601 taattaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     3661 acccgctaaa accccctgac gcgccctgac gggcttgtct gctgacgaaa acgcccacgg
     3721 gaaagccgtg accgcctccg tcagcttcgt tatgttcctg tttcactttc ggcctcaaag
     3781 cgaatctgga tgctgttctg
//
LOCUS       DADKXM010000228         3795 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.231, whole genome shotgun sequence.
ACCESSION   DADKXM010000228 DADKXM010000000
VERSION     DADKXM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.231"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JQR05_003939"
     CDS             complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JQR05_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY5325918.1"
                     /translation="MRNKADNKKRNFLTHSEIESLLKAANTGPHATRNYCLILLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     "
     gene            1933..2650
                     /gene="nanC"
                     /locus_tag="JQR05_003940"
                     /pseudo
     CDS             1933..2650
                     /gene="nanC"
                     /locus_tag="JQR05_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
     gene            2670..3776
                     /locus_tag="JQR05_003941"
     CDS             2670..3776
                     /locus_tag="JQR05_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY5325919.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSYAPMGMAGHVTFVHNGKAYVTGGVNQNIFNAYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT         1191 a          750 c          777 g         1077 t
ORIGIN      
        1 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc
       61 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
      121 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
      181 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
      241 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
      301 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
      361 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
      421 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
      481 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
      541 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
      601 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
      661 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
      721 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
      781 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac tagccgtctc
      841 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
      901 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
      961 ttcgtcagct taggccatga gattgttaca ttccttttta tcctagagat atgggggaac
     1021 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
     1081 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     1141 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
     1201 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta
     1261 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
     1321 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
     1381 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
     1441 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     1501 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     1561 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     1621 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
     1681 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
     1741 atctggatct ctgtttattt aaataatgtg aaaagagaat tttcacagga gaccttatac
     1801 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
     1861 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
     1921 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
     1981 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
     2041 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
     2101 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcacatcaa
     2161 tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga cggtgcgccc
     2221 gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc
     2281 ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta
     2341 ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata
     2401 tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa
     2461 acagaatgat tatcgctatg caaaccacaa gaaatgggca acggaaaatg cctttgttct
     2521 acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc ttgaccgcca
     2581 gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt
     2641 taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg cttgctatca
     2701 tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg ccatttaaaa
     2761 gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacag
     2821 catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta gctgcattcc
     2881 ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg tatgtgtttg
     2941 gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta cacaaataca
     3001 accccaaaac caatagctgg gttaaattga tgtcgtacgc gccgatgggc atggcgggtc
     3061 atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct
     3121 tcaatgccta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct atagataaaa
     3181 tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag tttctgttgt
     3241 cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg tacggaacgg
     3301 ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac
     3361 caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga
     3421 ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca
     3481 atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa aattaccaga
     3541 acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat attcatcttt
     3601 ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat
     3661 cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc ggcaaagcgg
     3721 tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa aactaacgct
     3781 tcagggcccc ggtaa
//
LOCUS       DADKXM010000229         3652 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.232, whole genome shotgun sequence.
ACCESSION   DADKXM010000229 DADKXM010000000
VERSION     DADKXM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.232"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..411
                     /locus_tag="JQR05_003942"
                     /pseudo
     CDS             1..411
                     /locus_tag="JQR05_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            996..1712
                     /locus_tag="JQR05_003943"
     CDS             996..1712
                     /locus_tag="JQR05_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325920.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGSYEINDVYVRK"
     gene            2264..2668
                     /locus_tag="JQR05_003944"
     CDS             2264..2668
                     /locus_tag="JQR05_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325921.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2731..3030
                     /locus_tag="JQR05_003945"
     CDS             2731..3030
                     /locus_tag="JQR05_003945"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5325922.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3027..3365
                     /locus_tag="JQR05_003946"
                     /pseudo
     CDS             3027..3365
                     /locus_tag="JQR05_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3407..3616)
                     /locus_tag="JQR05_003947"
                     /pseudo
     CDS             complement(3407..3616)
                     /locus_tag="JQR05_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1009 a          766 c          850 g         1027 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcaccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt
      301 gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt
      361 atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat
      421 tgtgggccat aaatattcca aaaaaatagc ccgctccgtt tattaccgga acgggcgaga
      481 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      541 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc
      601 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      661 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      721 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      781 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      841 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      901 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      961 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
     1021 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
     1081 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
     1141 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
     1201 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
     1261 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
     1321 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
     1381 tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1441 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1501 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1561 gttataataa tagttggtat tatgttcttg ctcgttatga ttcacggctc gttttgtcta
     1621 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatctt
     1681 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaat
     1741 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1801 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1861 cagtaaaaca ctcagggggg actcagtcca gattatgtaa tcagataaat tgccaaacat
     1921 cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1981 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     2041 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     2101 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     2161 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     2221 gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata ctgcgctgat
     2281 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     2341 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     2401 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2461 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2521 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2581 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2641 tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca cgcggctaag
     2701 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2761 acacgtaaac agcattcacc tgaattttgc agtgaagccc tgaagcttgc tgaacgcatc
     2821 ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat ctcagctcta tgcgtggcgc
     2881 agcaaactgc agcaacagat gacctcgtct gagcgtgaaa gtgaactggc cgctgaaaat
     2941 gcccgcctga agcggcaact ggcggagcag gcagaggaac tggctatcct ccaaaaggcc
     3001 gcgacatact ttgcgaagcg cctgaaatga agtatgtctt tattgaaaaa catcaggctg
     3061 agttcagcat caaggcaatg tgccgcgtgc tccgggtggc ccgcagcggc tggtatacgt
     3121 ggtgtcagcg gcggacaggg ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg
     3181 ttgtcctcgc ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg
     3241 atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc
     3301 gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac cgcgcacatg
     3361 gcctgaggtg tactggcaat agcggacacc accatttgtt ctttttctaa gcagtcatct
     3421 gataatattt tatcctgaag gctgccgggg agatattccc cagacgagag tgacgacgct
     3481 gacgattgta gaaaatctca atgtattccc gtattactga gatggcctca tcccggctct
     3541 taaaacgata gtggctcagg ctttcatttt tcagcgttcc ccagaagctt tccatcggag
     3601 cgttgtcgta acagtttgag gtagcctgag tttaacggac actccttcct ga
//
LOCUS       DADKXM010000230         3645 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.233, whole genome shotgun sequence.
ACCESSION   DADKXM010000230 DADKXM010000000
VERSION     DADKXM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.233"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /gene="aldA"
                     /locus_tag="JQR05_003948"
     CDS             <1..1064
                     /gene="aldA"
                     /locus_tag="JQR05_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY5325923.1"
                     /translation="IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTG
                     NTIVIKPSEFTPNNTIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMT
                     GSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCN
                     CAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARA
                     VEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAIS
                     MANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGA
                     DGKHGLHEYLQTQVVYLQS"
     gene            complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JQR05_003949"
                     /pseudo
     CDS             complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JQR05_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2296..2826
                     /gene="cybB"
                     /locus_tag="JQR05_003950"
     CDS             2296..2826
                     /gene="cybB"
                     /locus_tag="JQR05_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY5325924.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3071..3244
                     /locus_tag="JQR05_003951"
     CDS             3071..3244
                     /locus_tag="JQR05_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325925.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JQR05_003952"
     CDS             complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JQR05_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY5325926.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT          934 a          818 c          913 g          980 t
ORIGIN      
        1 ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
       61 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
      121 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatacga
      181 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
      241 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
      301 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
      361 ccaaagtgtg tctggaatta ggtggtaaag caccggctat cgtaatggac gatgccgatc
      421 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
      481 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatcggctgg
      541 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
      601 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
      661 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
      721 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
      781 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
      841 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
      901 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
      961 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     1021 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     1081 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     1141 gctgcgtgac gaagccatat tcgttatcgt atcaggcgac cgttttcacc agttgtaaat
     1201 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     1261 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     1321 cttgtttaag tgcgttattc acctcttcgg cagtcacttt ttttccgaga atggacacca
     1381 gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc agtttgccgc
     1441 tcagttccgg gatcaccaga ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat
     1501 tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg tgggccatcc accagtgact
     1561 gggtgccagt ataggcatga atggtcgtca tcgtgccgac ttctatcccg aaactgtcat
     1621 gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg gaaacaatgg
     1681 tgtcgttgcc atccagagtg tcgtcattaa cgttataaac gatggttttc atttcaccgg
     1741 caggagcgga aatcaacacc ttcttcgcac cagcatcaag atgcgcctgc gatttatcgg
     1801 cggaggtata aaagccagta cattcgacaa tgatttctgc ccctttcgct ttccacggaa
     1861 tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg ataagtgaat
     1921 cttccgtaaa atcaacgctc caggggaacg gaccatagtt tgaatcatgt ttcagcagat
     1981 aggcgagaat ttttggggaa gtaagatcat taatagcgac aacgtctatg ttgcttttga
     2041 cttcaagtaa acgacgcaac accagtcgac cgatacgacc aaaaccgtta ataccaactt
     2101 tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt ccaggcttag
     2161 accaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt actgaaaacg
     2221 ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga aaattatgtt
     2281 aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat tcactggctg
     2341 gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcgtggttt cttcccacgt
     2401 agcgatcggc cgctcatcaa catgattcat gtttcctgtg gcatctcaat tctcgtgctg
     2461 atggtggttc gtcttttgtt aagactgaaa tacccaaccc cgccgattat acctaagccg
     2521 aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct cctgtttatt
     2581 gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt
     2641 ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag cttaaagtcg
     2701 tggcatgaga cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc tgcggctgca
     2761 ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc gcgtaaacgt
     2821 tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta caggcgaaga
     2881 atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata aaaccataat
     2941 ccttgcccat acgtccatct ggcttatttt taatcaattc acccgatctt tgatctcatc
     3001 aacggtatca aaataaacca gcgtataacg ttcaaatcgc acataagatt cgataaacca
     3061 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
     3121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
     3181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
     3241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcagatgc gcggggaaaa
     3301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
     3361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg ccagaatcgt
     3421 aatgcagata atgagcagac acaccaccag agggttgcgc ttcatagcct ttctccttgc
     3481 cggatggcgg gtaagaggct aacatctgaa ttgcttggtt cattcgttgg cctcggtttg
     3541 tactgacccc aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc
     3601 atttggagtc agacctttta atttcaggct aattcttctg ctgtt
//
LOCUS       DADKXM010000231         3588 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.234, whole genome shotgun sequence.
ACCESSION   DADKXM010000231 DADKXM010000000
VERSION     DADKXM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.234"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(623..2332)
                     /locus_tag="JQR05_003953"
     CDS             complement(623..2332)
                     /locus_tag="JQR05_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY5325927.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2765..3484)
                     /locus_tag="JQR05_003954"
     CDS             complement(2765..3484)
                     /locus_tag="JQR05_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY5325928.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1069 a          718 c          639 g         1162 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggtatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcttg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcgctat aagatagcga gctcgtcaac gatacagatg
     2461 atctgattat attgtgcaaa aggagaacag gaaaataggc actggaaaag atgaaggcct
     2521 gatgggagca ttaactaatg caaaatccga caggttaata acccgaaaag caatcaaacg
     2581 actcaaacag gagatgcacg ctgaagtggt ggacaactgt tcggatttca gatgactagc
     2641 tcagcagttt atgagtcgaa tgctctgtca cgggcaggaa tcaggctgca tacgctgtgc
     2701 caaatctaat cgtagttatt ctaaccagtt ttcaagctac aaggtggtgt agctggcatc
     2761 ttctctactc tatcatcaaa cgataaaatg gttcctctcg taacatttgc tcttgccttt
     2821 cacttccatc tcgatcgctt cgtagttcat tacgatagct gacatatttc cagtcttcag
     2881 gtctaacatc tgacgccgga tactccccgg gagcgatttt gcttcttaat agattagact
     2941 cgagacacat tataaattgt cgtattttat gaagtaataa agcactatat tgcgagcatt
     3001 cctgatctga ttttacatat agcgtaaact gagctcctat ccccacacga gattgctgag
     3061 aaactcgatt catatcagtt atcttccatt tatctatagg actgtcctct gaaaataata
     3121 aaccagacaa aatttggaat gccagaggaa cttgctccct agctatacta atatgaaact
     3181 tgtccccaac aaaatccccc ttactctgag gattttcacg acatgcatga ataaacacat
     3241 ctccactatg gttcacaatc tggaatcctt ggtacatgac agaaaaaaat tgacatatat
     3301 cagagtaagt tggcacgttc gatgctactt tgaaatagta cgctgggtat tgccgtattt
     3361 ggttgtacaa tatattgaag ttatttttca atctttcatt cgcagaaagc atctgcggta
     3421 tttcagacct cacaacattc aaagaatcta aatttagttt tagacagggc ttttttatgg
     3481 gcatagaaaa cgtcctctat aaaatagata tattatctta tattgattgt ttatcatctt
     3541 ttataaaacg atacattata tgtaatgtat gtaagcgtaa gggtcttc
//
LOCUS       DADKXM010000232         3565 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.235, whole genome shotgun sequence.
ACCESSION   DADKXM010000232 DADKXM010000000
VERSION     DADKXM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.235"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..236
                     /locus_tag="JQR05_003955"
     CDS             <1..236
                     /locus_tag="JQR05_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY5325929.1"
                     /translation="EVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADY
                     PHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            246..1787
                     /locus_tag="JQR05_003956"
     CDS             246..1787
                     /locus_tag="JQR05_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY5325930.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRWVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGLHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            1784..>3565
                     /locus_tag="JQR05_003957"
     CDS             1784..>3565
                     /locus_tag="JQR05_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY5325931.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSKLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFTLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNSGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRLGGAALNAGQVIAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALVNENGMALVTVKEKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEITELFMWRHMALTSAA"
BASE COUNT          845 a          898 c         1005 g          817 t
ORIGIN      
        1 atgaagtggt agaaaaagaa caggtggaaa gctggatggc gaaccagtta gcgcagctgg
       61 aaaaatgcgg tccggtggca ctggcgcata ttaagcaact gattctggca ctggaaaata
      121 aacatgccga ttacccgcac cagctactgg ccggattaat gtctgccact caggattgcc
      181 agcaggcgac gcgcgccttt gccgaaaaaa gtagcgtgag cttccataat caataaggaa
      241 attgtatgcg taagataaca accgccgagg cactggcggc gcaaattcag gacggtgcga
      301 ctattgctat tagcggtaac ggcggcggta tggtagaagc cgaccatatt ctggctgcta
      361 ttgaagcgcg tttcctgcaa accggacacc ctcgcgacct gacgctaatc cattcgctgg
      421 gtattggcga tcgcgactgc aaaggcacta accgttttgc gcacgcagag atgctcaaac
      481 gcattattgc cgggcacttt acctggtcgc ccaaaatgca ggcgctggta aaaaataaca
      541 cgattgaagc ctactgcttt cctggtggcg tgattcaggc actgctacgg gagatcggct
      601 ccggacgtcc ggggcttttt acccacgttg ggctgggatc gtttgttgat ccacgcaatg
      661 gcggcggtaa gtcgaatgaa tgcactaccg acgacctggt agaactgatt gaaatcgacg
      721 gtgaaaccaa acttcgttat cgccctttca aagtggatta cgcgattttg cgtggcactt
      781 atgccgatcc tcgaggcaac gtcagccttg aagaagaagc gattgatatg gatagctatt
      841 ccatggcgct ggcagcacac aacagcggcg gcaaagtgtt cgtacaggta cgcgatgtgc
      901 tggaagctgg cgcgattgaa ccacgctggg tcaaattacc gggcattctg gttgatggca
      961 tcgttgaaca ccgcgaacaa ccacaaacct atcttggcgg ttacgacctg accatcagcg
     1021 gccaacatcg ccgtctgagt tctaacgacg ccattgaact ggttagtcat ccggtacgtc
     1081 gcctgattgc ccgtcgggcg gcacgcgaac tggtagctgg cgcttcaacc aactttggct
     1141 ttggtattcc tgggggtatt ccaggcgtag cgctgcgcga aggcgtgcct taccaaagtt
     1201 tatggctgag cgtcgagcaa ggcctgcata acggtatgat gctggatgat gcgtttttcg
     1261 gctgcgcccg caacgccgat gccattattc catcactcga tcaatttgag ttctacagtg
     1321 gcggagggat cgatatcacc ttcctcggca tgggagagat ggatcagtac ggtaacgtca
     1381 acgtatccca tctcaatggc aatctcattg gtcccggcgg ttttctcgaa attgcgcaaa
     1441 acgcccgtaa agtggtgttc tgcggcacgt tcgacgccaa aggtagcaag attgatgtaa
     1501 cgccagatgg cttgcatatc acccagtcag gtcaaatccc taaactggtt acccaggtgg
     1561 aaaaaatcac ttttagcgcc gcctacgcac agcaaagtgg tcaggaagtg ttgtatatca
     1621 ctgaacgcgc agtattccag ttaacggcag aaggcgttga attaattgaa atcgcgccgg
     1681 gtgtggagat tgagcgcgac attctgccgt atatggcttt ccgtccaatt atcaagcacc
     1741 cacgcctgat ggaaagtagc ctgtttacgc cgatggagga tgcatgagtc agcttgatga
     1801 caccatcctt gatgcgctga cgcacgttac tttcccaaag ggttttgcac aggcagagcc
     1861 cgcatgggtt gtcacggtag acggtgttga ttacccactc tggcaaacag atgccctggt
     1921 agtcggcagt ggtgcagccg ggctgcgtgc agctgtggaa cttaaacgcc gccagcaaaa
     1981 tgtgctgatc gccaccgccg ggttatatat ggggacgtcg gcatgttctg gttccgataa
     2041 acagacactg tttaccgccg ctaccgcggg caacggcgac aacttcacca aactggcaga
     2101 ggcactggcg agcggtgggg cgatggatca cgacaccgct tatgtcgaag cggtaggttc
     2161 tctgcacact cttggcgggt tgcaatatct tggtctggaa ttaccggaag atcgctatgg
     2221 cgcgattctt cgttatcaaa ccgaccatga cgaagccggg cgtgcaacct cgtgtgggcc
     2281 gcgtacctca aagttaatgg tgaaagtgct gttggaagaa gtacagcgcc tcgccattcc
     2341 agtgttgacc agtgcaacag tgattaaact gctgcatcag cgtgacgaaa acggcgaaga
     2401 ccgtgtggcg ggggcaatcc tcgcgaccgg tcatcgcgcc cataaccctt gggggctggc
     2461 aattgtgact gcgcccaatg tggtactggc aacgggcgga ccgggcgagc tttatcgcga
     2521 cagtgtttat ccacataaat gcttcggctc gctggggctg gcgctggagg aaggtctgac
     2581 gctaaccaat ctgaccgaaa gccagtttgg tataggcaca ccgcgcagca cgtttccgtg
     2641 gaatttgtcc ggcacctatg tgcaggtgat cccgtatatc tattccgtgg atgctgaggg
     2701 taacgagtat aacttcctcg cggattacta tcgcaccacc caagagctgg cttcaaacat
     2761 cttccgtaaa ggctatcagt ggccgttcca cgccacgcgg gtaatggatt ttggctccag
     2821 cttgttagat atggcggtgg cgcaagagca gcaacatggt cgtcgggtgt ttatggattt
     2881 caatcgcaat cctgaagcgg tgcctggtga tttgcccttc acattagatc gactggacga
     2941 cgacgttcgc gcgtatctgg aaaataatga cgctctggca ccatcgccca ttgaacggct
     3001 gcaacgaatg aatccgctgt ctatctcgct gtataagatg cacggttacg atctcaccac
     3061 gcagccattg cagtttgcca tgaataatca gcacatgaat agcggcattg aagtggatat
     3121 ctggggacaa acatccctgc ccggttgttt tgcggtggga gaagtagccg gaactcacgg
     3181 cgttacccga ctaggcggtg cggcgctgaa tgccggacag gttattgccg tgcgtctggc
     3241 gcgctttatt ggttgcacac aaaagcggtc tgttgagggt gatgtcgcgc agctggtagc
     3301 ctcatctctg aatatagtca gagaagttat cgctcaggca ctggtaaatg agaacggaat
     3361 ggcgctggta acggtgaaag aaaaaattca ggcgcgaatg tctgattacg ccggatttat
     3421 ttgtcatgcc gataaagttc gtcacgccac ccgtgacgcc ctgctgctaa gtgagtttgt
     3481 acaacaacac ggtttaacca tcaatcacgc aggcgaaatt accgaactat ttatgtggcg
     3541 gcatatggcg ctgacttctg ccgcc
//
LOCUS       DADKXM010000233         3562 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.236, whole genome shotgun sequence.
ACCESSION   DADKXM010000233 DADKXM010000000
VERSION     DADKXM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.236"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..591
                     /locus_tag="JQR05_003958"
     CDS             <1..591
                     /locus_tag="JQR05_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5325932.1"
                     /translation="LPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQE
                     KIAQLPNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYE
                     KVEIIRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPS
                     AFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            593..1657
                     /locus_tag="JQR05_003959"
     CDS             593..1657
                     /locus_tag="JQR05_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5325933.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(1700..1900)
                     /gene="glgS"
                     /locus_tag="JQR05_003960"
     CDS             complement(1700..1900)
                     /gene="glgS"
                     /locus_tag="JQR05_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY5325934.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            2169..2798
                     /locus_tag="JQR05_003961"
     CDS             2169..2798
                     /locus_tag="JQR05_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY5325935.1"
                     /translation="MNLFADHNTPYLFAISFVLLIGLLEIFALICSHMLSGTLDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLNGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLQEPEEGKIFTKGDKVLIICRLSATRYLVENNPWPQIL"
     gene            2825..>3562
                     /locus_tag="JQR05_003962"
     CDS             2825..>3562
                     /locus_tag="JQR05_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY5325936.1"
                     /translation="MDDIVNSVPSWTFTAIIAVCVLFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIE"
BASE COUNT         1070 a          737 c          728 g         1027 t
ORIGIN      
        1 ctaccctatc tggcgcaaac atggatagaa gatacgaaag gcaataaatc acgtgacttt
       61 attgtcacgg ttccccctat ggtacgttta aatcccagtg agcaaattca aataagaatg
      121 ataagtcagg aaaaaattgc tcaactgcct aatgacagag aaacgctctt ctattttaac
      181 gtgcgagaaa taccgccaaa aacagataaa aaaaatgtga tgcaagtcac tatgcagcac
      241 gcattgaaac tattctggcg accaaaagct attgaattag aagatgatgg ggtaatgtct
      301 tacgaaaaag ttgaaatcat ccgtagaaat gatggttcga tccgctttaa caataagatg
      361 ccttatcacg ttacgctggg ttatatcgga acaaacggaa tcacaatgtt gccacaaacg
      421 caaagtttaa tggtaacccc ttatagccat gcagatacgc agtttaaaaa tgttccgtca
      481 gcttttcagg ttggttatat caatgatttt ggtggattaa gtttttatga aataaactgc
      541 ccgacggtta ataactcttg taatgtttcc gtagccaaac gagataaata atatgcgcta
      601 cttattaatt gtaattatat ttattatagg ttttagtgca ttacctgtat gggcaatgga
      661 ctgctatgct gaacatgaag agggaaatac cgtagtcatt ggttacgtac caagaatttc
      721 tatccccagt aatggtaaaa aaggtgataa gatctggcaa agcagtgagt attttatgaa
      781 tgtcttctgt aataatgcat tacctgcccc atcacctgga gaagaatacc catcagcctg
      841 gactaatata atgatgtttt taccaggcgg tcaggacttt tataatcaga actcctatat
      901 ttttggcgta acatacaatg gagttgatta tgattcgact gcccctaatg ctatcgcagc
      961 accagaatgc attgatataa aaggagcggg tacctttaat aatcactata aaaaccccgc
     1021 tgtctgtagt ggaggtcctg agcctcaatt atcagtaacc tttccagctc gcgtacaact
     1081 ctatattaaa ttagcaaaaa atgccaatag agttaataaa aacctggtgt tgcctgatga
     1141 atatatcgct ctagagttta aaggtatgag tggcgcagga gctatagaag tagacaaaaa
     1201 tttgaccttc aggattcgtg gattaaataa tattcatgtc cttgactgct ttgttaatgt
     1261 cgcactggaa ccagctgatg gcgttgttga ctttggtaaa ataaattccc gaacaattaa
     1321 aaataccacc gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg
     1381 tactgagcaa ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag
     1441 ccatctggat atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac
     1501 ggaatttaac cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac
     1561 cgcaccattc agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt
     1621 tagtaaagac gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg
     1681 ggccatcagg cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt
     1741 ggcaatacca ggcataacgt gcgcaaaacc aggttctatg ttcttcatcc atgttaccag
     1801 taacggccag aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca
     1861 ggaaatcgaa attattcaaa gaattaagac tatgatccat aagcacatcc tccggttatc
     1921 gcataccttt taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc
     1981 ctttaaacat aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa
     2041 aaaccatatt aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa
     2101 gacaaatgta agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg
     2161 agtgacatat gaatttattc gccgaccata acacccctta tcttttcgct atttcctttg
     2221 tactgttgat cggtctttta gaaatttttg cgcttatctg tagccacatg ctctccggta
     2281 ctcttgatgc acatcttgac cattatgatt ccataaccac aggtcatatt agccaggcac
     2341 ttcattatct caatatcgga agattgccag ctctcgtcgt tctctgctta ctcgctggtt
     2401 tttttggtct taatggcatc ctgttgcaac atgcctgcat catggtctgg caatcaccac
     2461 tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac gattattgcg gtgcattata
     2521 ccggtaaaat agttgcgccc tggattccac gcgaccatag ttcagctatt acagaagaag
     2581 aatatattgg tagcatggcg ttaattaccg gtcatcaagc cacttcaggt aatccctgcg
     2641 aaggaaaact caccgatcaa tttggccaaa tacattattt actacaggag cctgaagaag
     2701 gaaaaatttt cactaaagga gacaaagtgt taattatttg tcgactttct gcaactcgat
     2761 atttggtgga aaacaatccc tggccccaaa tattgtaatt tattaatcaa aaggaaattt
     2821 aataatggat gatatcgtta attctgtgcc ttcctggacg tttaccgcaa ttattgccgt
     2881 atgcgttctg tttattattg gaattatttt cgccaggctc tatcgtcgcg cttcggcaga
     2941 gcaagcattt gttcgtactg gtttaggtgg gcaaaaagtg gtgatgagcg gtggcgcaat
     3001 cgtgatgccg atctttcatg aaataatccc catcaatatg aatactttga agctggaagt
     3061 cagccgctca accattgata gcctgattac gaaagatcgt atgcgtgtcg atgtagtagt
     3121 cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc gccactgcag cgcagacgtt
     3181 ggggcaacgc acgctatcac cagaagactt acgtatgttg gttgaagata aatttgtcga
     3241 tgccctccgt gcaacagctg cacaaatgac catgcatgag ttacaggata cccgcgagaa
     3301 cttcgtgcag ggagtacaaa atactgtcgc agaagacctg tcgaaaaacg gtcttgagct
     3361 ggaaagcgtt tcacttacca actttaacca gacctcgaaa gaacatttca atccgaacaa
     3421 tgcctttgac gccgaaggtt taaccaaact gactcaggag acggagcgcc gtcgtcgcga
     3481 acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt gagaaaaacc gtgatgcact
     3541 ttcgcgcaag ttggagattg aa
//
LOCUS       DADKXM010000234         3558 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.237, whole genome shotgun sequence.
ACCESSION   DADKXM010000234 DADKXM010000000
VERSION     DADKXM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.237"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..692
                     /locus_tag="JQR05_003963"
     CDS             <1..692
                     /locus_tag="JQR05_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5325937.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPCELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            794..2248
                     /gene="amn"
                     /locus_tag="JQR05_003964"
     CDS             794..2248
                     /gene="amn"
                     /locus_tag="JQR05_003964"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY5325938.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPSKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2591..3307
                     /locus_tag="JQR05_003965"
     CDS             2591..3307
                     /locus_tag="JQR05_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5325939.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          969 a          772 c          789 g         1028 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgtgc gaacttttcc
      241 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgg cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaaatatat
      901 tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg tctatccatc
      961 actgaccgta acctgggacg gtagcacaac aaatccctcc aaaacgcgcg catttggtcg
     1021 ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
     1081 tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct ctgtgcaacc
     1141 ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
     1201 ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc aaatcggcga
     1261 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
     1321 gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc cagtagaaca
     1381 ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
     1441 gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg
     1501 gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     1561 aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta ttactctggt
     1621 gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg cagtgctacg
     1681 cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagcc aggccattgg
     1741 agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc
     1801 gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg acgctaccaa
     1861 gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt
     1921 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     1981 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     2041 ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg gtgagattaa
     2101 gcttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc tgcaaattgg
     2161 cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg
     2221 aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca gaaatagatg
     2281 tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgcgggct gcttgcttcc
     2341 ttgggcagat gcgagtttta ttatcgtctt aatgatttcc acatattaaa agcaagtatg
     2401 ctttcaaaac acaattataa aaaatcccgc caacaacatt agtttttata aaattaaata
     2461 taagattatg gctttagaat atttttattt ctaatagacg agatttttcc tgttatgata
     2521 taatatgtcg aattaacaca tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga
     2581 gatcattcca gtgggacgta aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg
     2641 tgcaacgtct aaaatttatg caaagttcgg tgtagaaatc tatgctgctg ccaaacaagg
     2701 tgaacccgat ccagaattaa acacatcttt aaaattcgtt attgaacgtg caaagcaggc
     2761 acaagttcca aagcacgtta ttgataaagc aattgataaa gccaaaggcg gcggagatga
     2821 aacgttcgta cagggacgtt atgaaggctt tggtcctaat ggctcaatga ttattgctga
     2881 gacattgact tcaaatgtta accgtacgat tgctaacgtt cgcacaattt tcaataaaaa
     2941 aggcggcaat atcggagcgg caggttctgt cagctatatg tttgacaata cgggtgtgat
     3001 tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt ttgcttgaag ctgaagttga
     3061 tgttcgtgat gtaactgaag aagaaggtaa tattgttatt tatactgaac ctactgacct
     3121 tcataaagga atcgcggcgc taaaagcagc tggaatcact gagttctcaa caacagaatt
     3181 agaaatgatt gctcaatctg aagttgagct ttccccagaa gatttagaaa tctttgaagg
     3241 gcttgttgat gcccttgaag atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa
     3301 tctctaatta tattttaaag aaatctgtct ttatggcaga tttctttaat ctcatataat
     3361 tcttataaaa aatataatgt ttaactcgtc atattgctta taaaaaatcc ccgttcccag
     3421 agaaataata tttattaaaa tggcaggtct tctttttctg attaccgaag acaaagtggc
     3481 aattacgtat agtttcccga taaagacgcg acagctacat cccgcataag gcatttttct
     3541 ctttatcttt gtacggta
//
LOCUS       DADKXM010000235         3533 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.238, whole genome shotgun sequence.
ACCESSION   DADKXM010000235 DADKXM010000000
VERSION     DADKXM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.238"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..454
                     /locus_tag="JQR05_003966"
     CDS             <1..454
                     /locus_tag="JQR05_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5325940.1"
                     /translation="LFVLNPANENLLRIMYIGAPLAKDRETLFFTSVRAVPSTTKREE
                     GNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKI
                     GGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            479..3078
                     /locus_tag="JQR05_003967"
                     /pseudo
     CDS             479..3078
                     /locus_tag="JQR05_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3069..>3533
                     /locus_tag="JQR05_003968"
     CDS             3069..>3533
                     /locus_tag="JQR05_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5325941.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGG"
BASE COUNT          972 a          777 c          896 g          888 t
ORIGIN      
        1 gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca ttggtgcgcc
       61 gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc cttcaacaac
      121 gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca aacttttctg
      181 gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc gttgcacttc
      241 gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc tgacagacct
      301 gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc ccctttgata aataccaatt
      361 ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca atgactacgg
      421 ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc ttcagttaag agagcgagat
      481 gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat cgtttattgg
      541 tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct ggttcgatcc
      601 tgcttttttc aaagatgatc cctcaatggt ggctgatttg tctcgtttcg aaaaaggaca
      661 aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa ttgtagatac
      721 gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct gtttgacgac
      781 tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta aacaactgga
      841 caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa cgtttaatgt
      901 gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta atgcccgtgg
      961 ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg gatattcgtt
     1021 tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca gctattttct
     1081 gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt caacatggag
     1141 tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc agcgggcggt
     1201 tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg attttttcga
     1261 tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc cagacagcct
     1321 gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga taacgattaa
     1381 gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg aaattagtga
     1441 tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag cggacggcag
     1501 cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc aggggcgaat
     1561 caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc aggagagcaa
     1621 atttgcccag gccacgttgc agtggggcgg accgtgggga acgacatggt atggtggtgg
     1681 acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc ttggcgattt
     1741 cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc aaagcgaaca
     1801 taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg gcactaattt
     1861 tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg acaccatgta
     1921 taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc cgatgtggtc
     1981 gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata tctcccagca
     2041 attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact ggcataccga
     2101 tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc tctcgctggg
     2161 ggtttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg tgtttgcact
     2221 aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct ataccaggaa
     2281 aaaaattacg cctggatgac ctctaacacc agtatcgata acgaagggca cattacacaa
     2341 aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag cgtgcaacag
     2401 ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta caaaggggcg
     2461 tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca acaactgaac
     2521 tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg gcaatcgctg
     2581 ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt ggcgaacagc
     2641 accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac ttcttatcgg
     2701 gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct tgaaaatgca
     2761 gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa tgcccatgcg
     2821 ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt tggcgcgata
     2881 gcgacgctgg acggcataca gactaatagc ggcattattg atgatgatgg ctcgctctat
     2941 atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc tcccgatcaa
     3001 atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat tacgcgaatg
     3061 gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga ctattttttg
     3121 ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tatcgggcag tgtcatacca
     3181 ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aactctggag gaagaagaaa
     3241 atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat gattattatg
     3301 tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc gccgcggatt
     3361 caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt gccgccaaag
     3421 ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac gtgggcacag
     3481 gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt ggc
//
LOCUS       DADKXM010000236         3526 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.239, whole genome shotgun sequence.
ACCESSION   DADKXM010000236 DADKXM010000000
VERSION     DADKXM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.239"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..858)
                     /locus_tag="JQR05_003969"
     CDS             complement(<1..858)
                     /locus_tag="JQR05_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY5325942.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGQVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQCFFRDHNI
                     TIEMTESDAE"
     gene            complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JQR05_003970"
     CDS             complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JQR05_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY5325943.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2888..>3526)
                     /gene="bglG"
                     /locus_tag="JQR05_003971"
     CDS             complement(2888..>3526)
                     /gene="bglG"
                     /locus_tag="JQR05_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY5325944.1"
                     /translation="HIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHCQFAIKRFQ
                     QNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSG
                     NMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSD
                     ESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT          930 a          946 c          801 g          849 t
ORIGIN      
        1 ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacactgcat
       61 atagccggga tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc
      121 ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacct gcccaccgag
      181 aagcatattg ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc
      241 actggcaacc agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc
      301 cagccccacg cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca
      361 taacgctact ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc
      421 gatgaccgcc cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta
      481 atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc
      541 gtaaaacgct aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc
      601 ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc
      661 cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg
      721 cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc
      781 atcttccagc caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt
      841 ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg
      901 ttcacctgcg cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt
      961 actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa
     1021 agaaatcagc cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa
     1081 tttgccgtcc agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc
     1141 tgactcaatg ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg
     1201 agaacgcact tcaccaaccg agggcagaat ggcgatacct ttacccaaca ggccactggc
     1261 aaacgtggta tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat
     1321 accgccctgc tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc
     1381 tggctgacgt ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat
     1441 ggcaatgaca ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat
     1501 ggtctgcatg aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta
     1561 gccaatgatg gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg
     1621 cggcaggttg acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc
     1681 tgatcccgcc accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc
     1741 cccgacctgc gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac
     1801 ggtgaagtta ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat
     1861 ttgccagaag ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag
     1921 ataaccggcg gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt
     1981 gacgggtgtg ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg
     2041 taaccacgca ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg
     2101 aataaccgat gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg
     2161 cgcatccgct ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc
     2221 accaatcacc atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag
     2281 gattatcggg aagaaccaaa acaaggcatc actggcgctg aataaaatta aataagtacc
     2341 actttgttcg gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc
     2401 ggttgccgcc atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg
     2461 gtttagcaga ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc
     2521 tgccacgctg ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa
     2581 ctgaccaccg ctttccacca ccataataat accgggggtc tttttcagta cctcagcttg
     2641 cgctttgctt tcatccttta acttaaaacg taatcgcgtt gcgcaatgca tcagactcac
     2701 aatgttatct gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg
     2761 ccctctaccg ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga
     2821 atcaagcaat ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa
     2881 taatatttca gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg
     2941 cgggtgaaat tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt
     3001 gccatgcttg cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa
     3061 ttgaagcatg ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat
     3121 aactcaagct ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt
     3181 cgcgcattaa ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga
     3241 ccagatgcat ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat
     3301 caataatggt taacgcttct tcccctagct ggaactcttt cgggtaaagc cgctggatat
     3361 cccacagcaa cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc
     3421 aatggtcagt tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta
     3481 aagagataag acgatcacag gttgccatca cctcaagagg aatatg
//
LOCUS       DADKXM010000237         3512 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.240, whole genome shotgun sequence.
ACCESSION   DADKXM010000237 DADKXM010000000
VERSION     DADKXM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.240"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..91)
                     /locus_tag="JQR05_003972"
     CDS             complement(<1..91)
                     /locus_tag="JQR05_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY5325945.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGG"
     gene            complement(91..306)
                     /locus_tag="JQR05_003973"
     CDS             complement(91..306)
                     /locus_tag="JQR05_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY5325946.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWLLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(423..498)
                     /locus_tag="JQR05_003974"
     tRNA            complement(423..498)
                     /locus_tag="JQR05_003974"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Thr,seq:tgt)
     gene            complement(504..578)
                     /locus_tag="JQR05_003975"
     tRNA            complement(504..578)
                     /locus_tag="JQR05_003975"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(543..545),aa:Asn,seq:gtt)
     gene            complement(839..1462)
                     /locus_tag="JQR05_003976"
     CDS             complement(839..1462)
                     /locus_tag="JQR05_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY5325947.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGLAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAGTR"
     gene            complement(1459..1647)
                     /locus_tag="JQR05_003977"
     CDS             complement(1459..1647)
                     /locus_tag="JQR05_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY5325948.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1644..2006)
                     /locus_tag="JQR05_003978"
     CDS             complement(1644..2006)
                     /locus_tag="JQR05_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY5325949.1"
                     /translation="MNEYRFTLPYPPSLNTYWRRRGSRYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKARLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(2003..2773)
                     /locus_tag="JQR05_003979"
     CDS             complement(2003..2773)
                     /locus_tag="JQR05_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY5325950.1"
                     /translation="MNAVIIANAEIVPIEFNGERVVTYQMIADIHGVPVATIQNGYAR
                     NSEYFIDREDTYFIDFHENTFRRGFEIPPRGMRVFTESGYLMLVKTLRDETAWRVQRE
                     LVRNYFRGQKEDKSALPDFTNPAEAARAWANEYEQKVKLEQQLAIAAPKVEFADRVGE
                     ASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVN
                     TNHGIQISFTTKITGCGQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2788..3024)
                     /locus_tag="JQR05_003980"
                     /pseudo
     CDS             complement(2788..3024)
                     /locus_tag="JQR05_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3099..3491)
                     /locus_tag="JQR05_003981"
     CDS             complement(3099..3491)
                     /locus_tag="JQR05_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004968782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY5325951.1"
                     /translation="MLETDQAWEVFEKLEDCYFSQTSKSAGHHEKKTSELSAKDANSL
                     VWLWDYANRSQALFRELYPALRQIQSNYSGRCHDYGHEFSYVIGMARDVLINHTRDVD
                     INEPDGPTNLSAWVRLKNKELPPSLHHY"
BASE COUNT          830 a          894 c          797 g          991 t
ORIGIN      
        1 taccacctgg gccagtgatt aacacagatg ctatagcaac agccccgcca ccaatagcaa
       61 ctgcaacagc cttgcgtaat gatggcgaca ttattcacct ctcgcagcct tacgcttgtc
      121 ttctcttatt ttgaaataca gatttgtcag ataagtcaga agccccagaa gcagacttcc
      181 cagcacacca atcgcagccc actgtgacgg actgacctga tcaagccact gtaaaagcca
      241 gtagccggca ctgccggcgg aggtgccgta ggcaatgccc gttgacattt tgtccataga
      301 tttcataacc tcacctccgc aaataacgga tggcgtagtt cttatattgg gaaggggaaa
      361 agaaggccgc agcgtaactg tcactgatga attcaggata gccagtggct acggctcagt
      421 tatggtgctg gttaacggac ttgaaccgct acccattcgt ttacaaggcg actgctctac
      481 cattagagct aaaccagcat atttggcggg acagcgtgga ctcgaaccac gataagaagg
      541 ttaacagcct tccgtaatga cctttatacg actgacccaa ataaaaaatc ccgaaagcag
      601 gctctattcc tatgtgctct cgagtttatg atgcgcatgt cagtgcgctt agcgaaaaac
      661 gccgtaaacc ctcgcccgac agggcgggga gcagtcacaa cttaaagatg aggtgggtaa
      721 aaaaccgaaa gaatgtgaac ggatataaac ctgccattct tgagtcaaat ttacccaact
      781 ttattcaaaa agtcaatatc atgccgttaa gatgttgcca tccgtggcaa tcatgccgct
      841 aacgtgtgcc cgcattcaaa atgttgtctg cgattgactc ttctttgtgg cattgcacca
      901 ccagagcgtc atacagcggc ttaacagtgc gtgaccatgt gggttgggtg aggtttggga
      961 ttagcatcgt tacagcgcga tatgctgcac ttgctggcat ccttgaatag ccgacacctt
     1021 tgcatcttcc gcactctttc tcgacaactc tcccccactg ctctgttttg gctatatcaa
     1081 ctgccagacc tgttccgtgg caatctctgc atcttgcgcc cggcgtcgcg gcactacggc
     1141 aataatccgc ataagcgaat gttgcgagca cttgcagtac ctttgcctta gtatttcctt
     1201 cgagctttgc cacaccacgg tatttccccg ataccttgtg tgcaaattgc atcagatagt
     1261 tgatagcctt ttgtttgtcg ttctggctga gttcatgctt accgcaaaat gcagccattc
     1321 cgaatccggc ttgtgattgc gccatcccca tagcagccat cacatcagta ccggaaagag
     1381 agtcagaagc cgtagcccgt ggtgagtcgc tcatcatcgg gctttttggc gaatgaaatt
     1441 tagctacgct ttcgagtctc atcgtcttcc cctcttgccc tgtttgacca tcaggacgcc
     1501 gttaactatt acgtgacgct cgcctttgct gtctcggttg tacttgagca ctgttcctct
     1561 tgcgcaggaa agcatcctcg ccacttcggt ctgattgcct cgtgtctgga taagaagctc
     1621 tggtatcgtt tgaattgtgg cgttcatacg ttctccagtt cggtgatttt tattccaagc
     1681 cttccgcctg gtactttcac accacgaatt acgcgaatgt catcgaattg ctcgtcgtct
     1741 tccgcaaatc cggcgtggat aagagagtcg agtagcccct tcaggatgtt atcgaggtcg
     1801 cggcggcggg agtctggaac gtctgcgatg actttgatgc ggagtcgtgc tttggtgaaa
     1861 atatctaact tgagttggcg gatgatttgc tgaacgtctt ttcggtattt ctggccttta
     1921 tcgctgatgt agtatcggct tccccgtctt cgccagtagg tattcagcga tggtgggtat
     1981 ggaagcgtga atctgtactc attcatgcag cctcccctgt tactttcagc attccgttat
     2041 cgagcagctt tctggtcagc cactgttggc cacacccggt gatttttgtg gtgaacgata
     2101 tctgtattcc gtgatttgtg ttgactgctg tttctttcac tgtgaaatag ccgcgatcca
     2161 tatattcctg cattggcaca ttgcgccggg aacctgaagc aataaggatt ttgtgatcac
     2221 gcatccacgc aaacagtttg tttgggccaa taccaacaac ctttgcaaag tttccaatca
     2281 aaattccgct ggcctcgcca acgcgatcgg caaactcaac tttaggtgcg gcaatcgcga
     2341 gttgttgctc cagcttcact ttctgctcat attcgtttgc ccacgctctg gcggcttcag
     2401 cagggtttgt gaagtcgggc aaggctgact tatcttcttt ttggccacga aaataattgc
     2461 gaacaagctc acgttgcact ctccacgccg tctcgtcgcg cagggtttta accagcatca
     2521 aatagcctga ttcagtgaaa actcgcatgc cacgaggagg aatttcaaaa ccccttctaa
     2581 aggtattttc atgaaaatca ataaaatatg tatcttccct gtctatgaaa tactcagagt
     2641 ttcgcgcata gccattttga atagtggcta ctggaacccc gtgaatatca gcaatcattt
     2701 gatacgtaac aacacgctcg ccattaaact caatcggcac gatttctgcg ttggcaataa
     2761 ttacggcatt catgcaacac ctccttcaac gacaataatg ctgtcgcgct taccttgttc
     2821 gccttcgaag acgtaatgct cgtactgaac attgaaccct aagttattga ttctttcgga
     2881 aacctcaaat tgaggatgcc ggataacacc atttttagcc agcgtttcga tggtacgttt
     2941 cacattgtca tgacgcttac ccaccaactc agcgatttca atgctggtca ttttgatggc
     3001 gttgctattt atcagctcgt tcattgtcat gtcctctcat attgaaaatt cagcaataaa
     3061 aaacccagcc gaagctgggt cgttgcgttg gcaatctgtc agtagtgatg tagtgaagga
     3121 ggtaattctt tgttcttaag tcttacccat gcagaaagat tcgttggtcc gtctggctca
     3181 ttaatatcaa catctcgtgt gtgattgatt aaaacgtctc tcgccattcc gataacatac
     3241 gagaactcat gaccgtagtc gtggcatctg ccggaatagt tagactgaat ttgtcttagc
     3301 gccggataca gttcgcggaa taatgcctgt gagcggttgg cataatccca caaccatacc
     3361 agactgtttg catcttttgc agaaagctcg ctggttttct tctcgtgatg acctgcgctt
     3421 ttcgaagtct ggctgaaata gcagtcttcc agtttttcga acacttccca cgcctgatcg
     3481 gtttcgagca ttttggcgtg gcgtgcggct cc
//
LOCUS       DADKXM010000238         3470 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.241, whole genome shotgun sequence.
ACCESSION   DADKXM010000238 DADKXM010000000
VERSION     DADKXM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.241"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(91..711)
                     /gene="tqsA"
                     /locus_tag="JQR05_003982"
                     /pseudo
     CDS             complement(91..711)
                     /gene="tqsA"
                     /locus_tag="JQR05_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            1123..1488
                     /gene="mdtJ"
                     /locus_tag="JQR05_003983"
     CDS             1123..1488
                     /gene="mdtJ"
                     /locus_tag="JQR05_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY5325952.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1475..1804
                     /gene="mdtI"
                     /locus_tag="JQR05_003984"
     CDS             1475..1804
                     /gene="mdtI"
                     /locus_tag="JQR05_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY5325953.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1843..2664)
                     /locus_tag="JQR05_003985"
     CDS             complement(1843..2664)
                     /locus_tag="JQR05_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY5325954.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYRHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(2764..2847)
                     /locus_tag="JQR05_003986"
     CDS             complement(2764..2847)
                     /locus_tag="JQR05_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325955.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(2940..3275)
                     /locus_tag="JQR05_003987"
     CDS             complement(2940..3275)
                     /locus_tag="JQR05_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY5325956.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT          871 a          798 c          835 g          966 t
ORIGIN      
        1 tatcgtatgg tttttcatat tcatacattt ttattaggga tttatagctg tttaactaag
       61 tgtggttaat tgcgtggtta atttgactta tgtaagcatg ctgatggctg ttttcagcac
      121 cagataatga gaaacactgt caatcgcacg ttgaatcgcc gccatccctt caaccggacg
      181 cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact tcgagcagca taaacagcac
      241 cgtcagcagc aataaaaata ttgatgacat ggcattagat aactgcgtca ataagttggt
      301 gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc agttggtcaa ctgagacgtc
      361 aatccctacg cgttgcaaca acggctcaag agcttgcagc ggcgtcataa tagagttgcg
      421 atattgcggt aacgtccgcg tcaactcgtt gagcgcggaa cccagataag ctaatagcaa
      481 caccatcgcc atcacgatga ttgtcatcaa aatcgacact gccagtacac gcggcacacg
      541 ccagcggacc atatgttgca ccagcgggtt aagaataaca gcaataaata atgcgagaat
      601 aaacggcacg atgatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc
      661 caacataatg acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc
      721 cgttttttct tcctttgcta ataataatgt ctttttcgct ccgtcatcat aacgaaaatc
      781 aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat tcttaagcta tgatacaaat
      841 caggcgtgtt caactaccga ggacaattat catctgcgat gacgagaagc aacactgcgg
      901 ataattgtaa tattatggac aatttgttca ggacgttatt ttctacattt acccatcttc
      961 gtacctcatc cactatcttg ctcgtaggtg agcagcactg gcgcaacgcg ctttagtcct
     1021 ttcttctgcc taagcttgcc ctatgcgcag ggcttgacca ctttactttg gtttcgctga
     1081 attaagcgaa aattaaaata gttctcttgc aggagaagga caatgtatat ttattggatt
     1141 ttattaggtc tggctattgc tacagaaatt accggtacgc tgtcaatgaa atgggcgagc
     1201 gtcagtgagg gaaatggcgg ctttatttta atgctggtga tgatttctct gtcgtatata
     1261 tttctctctt tcgccgttaa aaaaatcgcc ttaggcgtag cttatgcgct gtgggaaggt
     1321 atcggtattt tatttattac cttgtttagc gttttgttat tcgacgaaag tttatcgctg
     1381 atgaaaattg ccgggttaac caccctggtc gccgggatag tgttgatcaa atcaggtacc
     1441 cgtaaagcgc gtaaacctga actggaggtg aaccatggcg cagtttgaat gggttcacgc
     1501 cgcctggctg gcattggcaa tcgtgctgga aatcgttgct aacgtctttt tgaaattttc
     1561 tgacggcttt cgtcgcaaaa tatttggctt gctctccctg gcggcggtgc tggcggcctt
     1621 tagtgcgctt tctcaggccg ttaaagggat cgacttgtct gtcgcttatg cattgtgggg
     1681 cgggtttggt attgccgcca cgttagccgc aggttggatc ttgtttggtc aacggttaaa
     1741 tcgtaaaggc tggattggcc tggtcttgct gttggctgga atgatcatgg tgaaacttgc
     1801 ctgatgaaga cgctgcccgc gctgtcgggc agcgtttgaa cattattttt gcgacagttg
     1861 atccagcttg tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc gccagcgatc
     1921 tttcgcggca ggagccgaac tttgcacccc aattaattgc cagccgtcat cggtatgcaa
     1981 catcagaggc gaaccgctgt cacccggcaa ggtatcgcac tgatgtgaca tcaccgacgt
     2041 ttgcgcccag ccagtcactt cacagttttg atgacggtat aacgtatcga gatgatcttc
     2101 agggtatccc gcctgagtca ctttacgacc agccgatttt aatgcggcag taagcgcggc
     2161 tttatctccc tcaaataacg gcaacggtgt aatgccagaa gggggattac gtagcacaat
     2221 caatccgaag tcccacggtg cagctgcggg aggaacaatc caaccatccc catctgcttt
     2281 taaccgcttt cccagtgtcg gatcaacgcg gccttctatg tcgtggatct catagcgcca
     2341 aagaccttta tttgacacaa aacgcagcgc taccgcctta tccgctttac ccttaggcgg
     2401 cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt gcaatcagcg tcgccgtaca
     2461 taaattgccg ctggccgttt ccagttgccc aaccgcatcc cacggtgatt gggtcgtgtc
     2521 attcactggc acacgatcat catgaccaaa aaacagggtg ctgacctcat cgtttgccga
     2581 tttggcaacg tctggtttat ctgcaaacac aaaagcagac gtcaaactaa ttgcacccaa
     2641 cactacagca atggttgtac gcatatcaca ctctggtggg taattatgat tattaaaagt
     2701 aaaccctcat aaatactata gacgggacgg gatgaaagtg ggagtaaaat cagataacta
     2761 cagtcaggaa agataaaaac gggcggcaat cagtgcgcat aaaataagga tgataagaat
     2821 gaactcaaag cgataacggc ccaccatact accctccgct aaaggcggtg ctaaacgtta
     2881 cccgcttagc accgtaattt tcaggcgcga gggggcgcgc cagcattact gttgaaaact
     2941 tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat ggccgtgttt cccggcgtgt
     3001 ttcttcgctg cctgcgcttt ttgttcaggg gctttctgtt cagctttcgt atttttatga
     3061 tgctttttag ccgcctgcgc tttctgggca ggtgctgctt tatgctgttt tttatgatgt
     3121 gtagttttcg ctggcgctgc tttggtggtc gtcgcagtcg gagcaggtgt ggtcgcagtc
     3181 tctgcagcaa aggcggcaga agacagaccc atagcagcgg caacaaccag agctaatact
     3241 tttttcattg tcataccctc aatttgtttt ttcatttaac cccactgcgg ggccgttgaa
     3301 ataactatat ccctgggaat ggcagacttc cgtgagtggt tggtttcagc gtgtaacgat
     3361 atgtacaaac gctgaataaa ttacagcgtt gataataacg tattgtgact taaggaaaat
     3421 ttagctacca ataatagtag tcttgatcgg gttaactatt atactactta
//
LOCUS       DADKXM010000239         3408 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.242, whole genome shotgun sequence.
ACCESSION   DADKXM010000239 DADKXM010000000
VERSION     DADKXM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.242"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            122..673
                     /locus_tag="JQR05_003988"
     CDS             122..673
                     /locus_tag="JQR05_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY5325957.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            733..3237
                     /locus_tag="JQR05_003989"
     CDS             733..3237
                     /locus_tag="JQR05_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5325958.1"
                     /translation="MYKKLKLTAISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DLENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPT
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTTMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDLGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNEISHTASWYNSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3253..>3408
                     /locus_tag="JQR05_003990"
     CDS             3253..>3408
                     /locus_tag="JQR05_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5325959.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSK"
BASE COUNT         1052 a          715 c          687 g          954 t
ORIGIN      
        1 tttagataaa ctaattttat aaaaatcaca aaacaaaata taatatttta atgattggta
       61 gtccttaaat aacagttact aatcattctc ttttttactt atctttatat aaaggaatat
      121 tatgtctgct tttaaaaagt cacttcttgt tgctggtgta gcaatgattc tttccaataa
      181 cgtgtttgct gatgaaggtc acggtattgt taaatttaaa ggtgaagtta tctctgctcc
      241 atgttctatt aaaccaggtg acgaagattt aactgtaaat ctgggtgaag tagcagatac
      301 cgtactgaaa agcgatcaga aatctttggc tgaacctttc actattcacc tgcaagactg
      361 catgttgagc cagggtggaa ccacttattc aaaagcgaag gttactttca ccacagcgaa
      421 tactatgacg ggtcagactg acctgctgaa aaacaccaaa gaaactgaaa tcggtggtgc
      481 cacaggcgtt ggcgtacgta ttctggatag ccagagcggc gaagtgactt taggtactcc
      541 ggtagtaatt acattcaaca atactaacag ctatcaggaa ctgaacttca aagctcgtat
      601 ggaatccccg ggcaaagatg ctaccccggg caacgtttac gctcaggctg actataagat
      661 tgcttacgag taatcacatt aggccggggt cgcccggcca catcattaac ggattaatga
      721 taagtggaac agatgtataa aaaattaaaa ttaaccgcaa taagcgaatt gattaaaaat
      781 atttattgtt cattatccgt tctcatcatt ggttgtgcgt cagcttatgc cgttgaattc
      841 aacaaagatt taatcgaagc cgaagatctt gaaaacgtta acctttccca atttgaaact
      901 gatggccaat tacccgtagg taaatactca ttaaacgctc ttattaataa taaaaggacg
      961 ccaatccatc tggacctgca atgggtatta attgataacc aaactgcagt ttgtctgact
     1021 ccagagcaat taacattatt aggctttact gatgagatta ttgaagaggc tcagcaaaac
     1081 ctgatcgatg gttgttaccc tatcgaaaaa gaaaaacaaa ttacaactta tctcgataaa
     1141 gggaaaatgc aattatccat atctgcacct caggcatggt taaaatacaa agatgcaaac
     1201 tggacgcctc ctgaactttg ggatcatggt attgctgggg catttcttga ctacaattta
     1261 tatgcctctc attatgcacc acatcagggc gataattcac aaaatataag ttcctatggg
     1321 caggctgggg ttaatcttgg ggcctggcgc ctgcgtactg attaccagta cgatcagtca
     1381 tttaacaatg gtaaaagcca ggcgaccaac ctggattttc cgcgtattta tttgtttcgc
     1441 ccaatcccag caatgaatgc aaaactaact ataggtcaat acgatactga atcctctatt
     1501 ttcgactctt tccatttttc tggcatttcg ttgaaaagcg atgagaatat gttaccgaca
     1561 gacctacgtg gttacgcacc gcaaatcacg ggtgtcgcac aaacgaatgc aaaggtcact
     1621 gtctcacaga acaaccgtat tatttatcaa gaaaatgttc ctccaggccc atttgctatt
     1681 accaatttat tcaatacatt acaggggcaa cttgacgtca aggttgaaga agaggacgga
     1741 cgcgttacgc aatggcaagt tgcatctaat agtattcctt atctgacgcg taaagggcag
     1801 attcgctaca ccactactat gggtaaaccg accagcgttg gtggtgattc cttacaacaa
     1861 cccttcttct ggactggtga attctcatgg ggttggctga acaatgtatc cctgtatggt
     1921 ggttcagttt taacaaaccg tgattatcaa tctctggctg ccggcgttgg ttttaacctt
     1981 aactcattgg gttcattatc ttttgatgtc acacgatctg atgctcagtt gcataatcag
     2041 gataaagaaa cgggttatag ctaccgcgct aactattcaa aacgttttga atctaccggt
     2101 agccagctca ctttcgctgg ttaccgtttc tctgataaaa actttgtgac aatgaatgaa
     2161 tatatcaatg acactaacca ttacacgaat tatcagaatg aaaaagagag ttatattgtc
     2221 acgtttaacc agtatcttga atcattaagg ttaaatacat acgtaagttt ggctcgtaat
     2281 acttactggg acgccagcag taatgtgaat tattcattat cacttagccg cgattttgat
     2341 ctcgggccat taaaaaacgt ctccacttct ctaacattta gccgaataaa ctgggaagaa
     2401 gacaatcagg atcaactgta cctaaatatt tcgattccct ggggaaccag tagaacattg
     2461 agctatggta tgcaacgaaa tcaggataac gagatttcgc atactgcttc gtggtataac
     2521 tcttccgatc gaaataattc ctggagcgtt tctgcttcag gcgacaatga tgaattcaaa
     2581 gatatgaaag cgtcactacg cgccagttat cagcataata ccgagaacgg tcgtctctat
     2641 ctctccggga catcacagcg agacagttat tattctctga atgccagttg gaatggttca
     2701 ttcacggcga ctcgtcacgg agccgctttc catgactata gtggtagtgc tgactcgcgt
     2761 tttatgatcg acgcagatgg tgcagaagat attccgttga acaataaacg cgcggtaact
     2821 aatcggtatg gcatcggagt tattccatca gtcagcagtt acataacaac atcattaagc
     2881 gttgataccc gaaatcttcc agaaaatgtg gatatcgaaa actcggttat caccaccacc
     2941 ttaaccgaag gtgctattgg ctacgccaaa cttgataccc gcaagggcta tcaaatcatg
     3001 ggggttattc gcctagcaga tggtagtcac ccaccactgg ggattagcgt aaaagataaa
     3061 accagccata aagaattagg actggttgct gatggcggct ttgtatacct caacggcatt
     3121 caggatgaca gcaaacttac tttacgctgg ggtgataaat cctgttttat tcaaccaccc
     3181 aatagcagca acttaaccac cggaacggtt attttaccgt gtattagcca aaattaattc
     3241 aggatgaaac taatgcgcta cttaaacaca aagaatctca ttgcagcagg agtattactc
     3301 gcctgcatga gcagcattgc ctggggtgcc atcatcccgg accgtacccg tatcataatg
     3361 aatgagtcag ataaagggga agcattaaaa cttactaatc aaagcaaa
//
LOCUS       DADKXM010000240         3243 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.243, whole genome shotgun sequence.
ACCESSION   DADKXM010000240 DADKXM010000000
VERSION     DADKXM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.243"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..345
                     /locus_tag="JQR05_003991"
     CDS             <1..345
                     /locus_tag="JQR05_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5325960.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
     gene            335..1129
                     /locus_tag="JQR05_003992"
     CDS             335..1129
                     /locus_tag="JQR05_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5325961.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1151..2575
                     /gene="patD"
                     /locus_tag="JQR05_003993"
     CDS             1151..2575
                     /gene="patD"
                     /locus_tag="JQR05_003993"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAY5325962.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSITTGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            2785..3138
                     /locus_tag="JQR05_003994"
                     /pseudo
     CDS             2785..3138
                     /locus_tag="JQR05_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
     gene            complement(3159..>3243)
                     /locus_tag="JQR05_003995"
     CDS             complement(3159..>3243)
                     /locus_tag="JQR05_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5325963.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          761 a          718 c          896 g          868 t
ORIGIN      
        1 atcctgcccg ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct
       61 gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg
      121 ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg
      181 cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg
      241 attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg
      301 tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg ctccgttttt
      361 cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc
      421 cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct
      481 gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact
      541 taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc
      601 gctgtggaga cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat
      661 tgcgctgccg ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct
      721 ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt
      781 taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga
      841 tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc
      901 gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac
      961 ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc
     1021 gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc
     1081 gatcctgggg gcctggtggc taacccgcga aggcgacaat ggtcaataac cactgataca
     1141 ggaataagct atgcaacata agttactgat taacggagaa ctggttagcg gcgaagggga
     1201 aaaacagcct gtctataatc cggcaacggg ggacgtttta ctggagattg ccgaggcatc
     1261 cgcagagcag gtcgatgctg ctgtgcgtgc ggcagatgca gcatttgctg aatgggggca
     1321 aaccacaccg aaagcgcgtg cggaatgcct gctgaaactg gcggatgtta tcgaagaaaa
     1381 tggtcaggtt tttgccgaac tggaatcccg taattgtggc aaaccgctgc atagtgcgtt
     1441 caatgatgaa atcccggcga ttgtcgatgt ttttcgcttt ttcgcgggtg cggcgcgctg
     1501 tctgaatggt ctggcggcag gtgaatatct tgaaggtcat acttcgatga tccgtcgcga
     1561 tccgttgggg gtcgtggctt ctatcgcacc gtggaattat ccgctgatga tggccgcgtg
     1621 gaaacttgct ccggcgctgg cggcagggaa ctgcgtagtg cttaaaccat cagaaattac
     1681 cccgctgacc gcgttgaagt tggcagagct ggcgaaagat atcttcccgg caggcgtgat
     1741 taacatactg tttggcagag gcaaaacggt gggtgatccg ctgaccggtc atcccaaagt
     1801 gcggatggtg tcgctgacgg gctctatcac caccggcgag cacatcatca gccataccgc
     1861 gtcgtccatt aagcgtactc atatggaact tggtggcaaa gcgccagtga ttgtttttga
     1921 tgatgcggat attgaagcag tggtcgaagg tgtacgtaca tttggctatt acaatgctgg
     1981 acaggattgt actgcggctt gtcggatcta cgcgcaaaaa ggcatttacg atacgctggt
     2041 ggaaaaactg ggtgctgcgg tggcaacgtt aaaatctggt gcgccggatg acgagtctac
     2101 ggagcttgga cctttaagct cgctggcgca tctcgaacgc gtcagcaagg cagtagaaga
     2161 ggcgaaagcg acagggcaca tcaaagtgat cactggcggt gaaaagcgca agggtaatgg
     2221 ctattactat gcgccgacgc tgctggctgg cgcattacag gacgatgcca tcgtgcaaaa
     2281 agaggtattt ggtccagtag tgagtgttac gcccttcgac aacgaagaac aggtggtgaa
     2341 ctgggcgaat gacagccagt acggacttgc atcttcggta tggacgaaag atgtgggcag
     2401 ggcgcatcgc gtcagcgcac ggctgcaata tggttgtacc tgggtcaata cccatttcat
     2461 gctggtaagt gaaatgccgc acggtgggca gaaactttct ggttacggca aggatatgtc
     2521 actttatggg ctggaggatt acaccgtcgt ccgccacgtc atggttaaac attaagttag
     2581 atgttagtgg ctgttatagg acggccacta tcgttttagg ttctgagttt aatggcggtt
     2641 aagtattaga atgattacat taataactag cgtaaaaatt aggctaaatg tttgatggcg
     2701 atttttcatc ctgtcacagt tgattagcat aaggctttag agtatcgtta ttaaactaat
     2761 aatttataag tagaagtatt aacaatgaaa gttcaattgt acaaattcac ggaagataaa
     2821 aataaaaccc ttaccttcag atggacgaaa aaacactttg agttttgtat ggataataaa
     2881 atatttttaa tatcataagg ggaagaagtc gtataaagaa aggaatttat ttcttttctc
     2941 aaaaggtgac aagataacaa ttgaagataa tgttattgct gaagaatatt caacaatgcc
     3001 tgtgaaaaat ttctccagtg tcggcgcttt ttcatttccg acctgccatt tttcagggaa
     3061 tatacgtata ggtcgatttt gtagtattga ataatgggtg gaaatcatcc tttgaatcgt
     3121 tttactactc acatgatgac ttaggtaatg actccaactt actgatagtg ttttatgttc
     3181 agataatgcc cgatgacctt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc
     3241 cgt
//
LOCUS       DADKXM010000241         3171 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.244, whole genome shotgun sequence.
ACCESSION   DADKXM010000241 DADKXM010000000
VERSION     DADKXM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.244"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..2075)
                     /locus_tag="JQR05_003996"
     CDS             complement(<1..2075)
                     /locus_tag="JQR05_003996"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY5325964.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIDQAGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKEMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADL"
     gene            complement(2096..2722)
                     /locus_tag="JQR05_003997"
     CDS             complement(2096..2722)
                     /locus_tag="JQR05_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY5325965.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGGLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT          844 a          792 c          662 g          873 t
ORIGIN      
        1 agatcggcgg caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca
       61 agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca
      121 cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc
      181 caggaacaga taagatcgtg ttcattacgc atctctttgg tttgcatcgg ttttaccgtg
      241 taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc
      301 tcttcgccag tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta
      361 cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta
      421 tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc
      481 gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca
      541 cgttgcagtt tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag
      601 tcacggttga acatacagtt aacaatggaa ccgacctgcg ctgacgcttc attggcatgg
      661 tgaaccggat agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta
      721 cccaaattcc agcgttctgc gatggcatat gaaccatcag ccaggtgact cagctcaccc
      781 acacgatgcg ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt
      841 tgatcccagc gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg
      901 ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca
      961 tcgaacaaat tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa
     1021 tctttcggcc cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca
     1081 cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg
     1141 atcgggcaag agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag
     1201 tcaaaaacgg atttataggt acgaaagccg accgtattct gattgcctgg cggaatttca
     1261 cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag
     1321 cgtgacttgg gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg
     1381 ttattcgcac caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga
     1441 tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc
     1501 attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga
     1561 ggaataaggt tttccccagc ctgatcaata gccgccacac aggtttccgg actggtcaat
     1621 cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttct
     1681 aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata
     1741 ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaaaccccc catatgggca
     1801 tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac gcgagaacta
     1861 cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt cgcttcatcg
     1921 aaaggtttcg tgcctagcgg tacttcgtca tacataattt tgtagccgaa gcccatgcca
     1981 ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt cgtgagattg
     2041 actcttaata tattacctgt ccaaccgtta gccatgattt ttttctttgc aagattcaca
     2101 cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg tttgcgcatt
     2161 caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg gccatcatcc
     2221 acgggcatgc cgtagtacag gcgctacagc caatacaacg tttatgatcg acggtaatac
     2281 agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg cattgcggct
     2341 ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg ccgccgccag
     2401 agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg gagaagaatg
     2461 tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg caacccgtgc
     2521 atcgcgctcg ctgggtcacc agcccccctt tgggtgtatt tatcaaccct actgtgccgc
     2581 tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca attgttagac
     2641 cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca atatctaata
     2701 gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag tgctgacaat
     2761 tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc caacaccaga
     2821 tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac taatccttta
     2881 tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg ttatcaatta
     2941 aaattacaaa gcaagaaatt ataagcgaac tgatatttat tatcatttga aataaattta
     3001 acttaagcga caatcatttg tgacgttcat tgcagaaaat aaaaataaca ttatcgctat
     3061 aaattaaaat acataccgaa attcgtataa tttcagcagt tgttatttag aatgattaac
     3121 tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc t
//
LOCUS       DADKXM010000242         3125 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.245, whole genome shotgun sequence.
ACCESSION   DADKXM010000242 DADKXM010000000
VERSION     DADKXM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.245"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            553..1314
                     /locus_tag="JQR05_003998"
     CDS             553..1314
                     /locus_tag="JQR05_003998"
                     /inference="COORDINATES: protein motif:HMM:NF012234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY5325966.1"
                     /translation="MNILPAGASVINDFSHHIDLSVEKAWMLASIRFGDQKIRSGWLL
                     LAWLTTPELCRVLKNICAPLAELPVDELLDILPSLITSSPELTEMPYDGSDLNSAVPG
                     EASQVFAGESTGSKSALAKYCQDMTALAREGNIDPVTGREHEIRTMTDILLRRRQNNP
                     LLTGEAGVGKTAVVEGFALAIAGGEVPPSLRNVRLLSLDVGALLAGASMKGEFESRLK
                     ALLEEAAHSPQPVILFVDEVHTLVVHPVRGMLPTC"
     gene            1422..2951
                     /locus_tag="JQR05_003999"
     CDS             1422..2951
                     /locus_tag="JQR05_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY5325967.1"
                     /translation="MQVAEPEEVAAVEMVRGLVTTLEKHHDVLILDEAVRAAVQLSHR
                     YIPARQLPDKAISLLDTAAARVALSLHTPPARVQYLRQQLNAAELELELLLKQEKMGI
                     RSERGDVLAARIISLNADLSDTEARWQQELELVHRLQEQRAAEPGERDEITLQQAETA
                     LRDWQGENPVVFPEVSAAVVAAIVSDWTGIPAGRMVKDEASQILELPARLAQRVTGQR
                     DALAQIGERIQTARAGLGDQRKPVGVFLLAGPSGVGKTETALALAEAIYGGEQNLITI
                     NMSEFQESHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELF
                     FQVFDKGAMEDGEGVHIDFKNTTLLLTTNAGAELISQLCEDPTLMPDSAGLKEALMPE
                     LRKHFPAAFLGRVTVIPYLPLDETARGVIARLHLGRLMDRMAEQHGVSLVYGDELITH
                     IVSRCPMHETGARLLIGYIEQHILPQLSRYWLQAMTEKTAITQIRIRVDDDEQVIFET
                     D"
BASE COUNT          736 a          737 c          865 g          787 t
ORIGIN      
        1 tatcactgtg tagagtgttt ctttcaaaag aataagtttt ctaaattttt agataagcaa
       61 tgctgatcaa tttactttat caatataagt tttcgcggaa atatatatgg cgatttttaa
      121 tttaatatat tcatggtgtg tatgaaattg gcttactcta ttgaatatta ccatgaaaaa
      181 gatactacct gctatattac attgtgttct gtgatatgca tcattgtttc attctgcctt
      241 tttgataatt ctattaatta gaagttttaa tcggtacagt tatgtttatg tatcatttat
      301 atgttattac atcatcaggt gtaacagatg gacactcctg tttcgcgcag tgcactatat
      361 ggaaagctaa ccaaatcttt gtttagatct ttggaatctg cgacaacgtt ctgtaaatta
      421 cgcgctaatc cttatgtcga actgacgcat tggttgcatc agttaataca acaaccagag
      481 aatgacattc acaatgtgct tcgacattat caaataacat ctgctgatgt tgagaaggcg
      541 ttaattcagc aattgaatat attgcctgcc ggagcgagtg taattaacga tttttcccat
      601 catatcgatc tttctgttga aaaagcctgg atgttggcaa gtatccgctt tggcgatcaa
      661 aaaattcgta gtggatggtt attacttgcc tggttaacca cgccagaatt gtgtcgggtg
      721 ttaaaaaata tttgtgcgcc attggcagag ctaccggtag atgaactatt agatattctt
      781 ccgtctttaa tcacgtcatc tcctgaatta acagaaatgc cttatgacgg ctcggattta
      841 aattctgcag taccggggga agctagccag gtctttgccg gcgaatcaac tggaagcaaa
      901 tccgcgctgg cgaaatactg tcaggacatg actgcgctgg cccgtgaagg caatatcgat
      961 ccggtcaccg gtcgcgaaca cgagatccgt actatgacgg atattctgct gcgccgtcgc
     1021 cagaacaacc cgctactgac cggcgaagcg ggagtcggga aaacggcagt ggtggaagga
     1081 ttcgcgctgg cgattgccgg gggggaagta ccgccttcac tgcgtaacgt gcggttgctg
     1141 tcactggatg ttggcgcgct gctggctggg gcgtccatga agggtgaatt tgaatcccgc
     1201 ctgaaagcgt tgctggaaga ggccgcgcac tcgccgcagc cggtaatcct ttttgttgat
     1261 gaagttcaca cgctggtggt acatccggta cgggggatgc tgccaacctg ctgaaaccgg
     1321 cgctggcgcg tggcaccctg cgcaccatcg gcgcaaccac ctggagcgag tacaaacgcc
     1381 atatcgaaaa ggatccggcg ctgactcggc gttttcaggt attgcaggtt gctgagccgg
     1441 aagaggttgc ggctgttgag atggtacgcg ggttagtcac cacgcttgaa aaacaccatg
     1501 acgtgctgat ccttgatgag gccgtgcgcg ccgccgtaca gctttctcac cgttacattc
     1561 cggcccggca actacccgat aaggccatca gcctgctgga tacggcggcg gcccgtgtag
     1621 cgctgtcgct gcataccccg cctgcgagag tccagtatct gcgccagcag ctcaacgctg
     1681 ccgaactgga gctggagttg ttattaaagc aggaaaaaat gggtatccgc tcggagcggg
     1741 gcgatgtact ggcggcgcgg attatatcgc tgaatgccga tctttctgac accgaagcgc
     1801 ggtggcagca ggaactggaa ctggttcacc gattacagga acagcgtgcc gcagaacccg
     1861 gcgagcgtga cgaaatcacg ctgcaacagg cagaaacggc attaagagac tggcagggcg
     1921 aaaaccccgt cgtgttcccg gaagtcagcg cggcagtggt ggccgcaatt gtttctgact
     1981 ggaccggtat tcccgccgga cgcatggtga aagatgaagc cagtcagatc ctggaactgc
     2041 ccgcccgtct ggcgcagcgt gttaccgggc agagggacgc gctggcgcag attggcgagc
     2101 gtatccagac cgcgcgggcg ggtttaggtg accagcgtaa gcctgtgggc gtctttttgc
     2161 tggccgggcc gtccggtgtt ggcaagactg agaccgctct ggcgctggca gaggctatct
     2221 atggcggtga gcagaacctg atcaccatca acatgagcga gtttcaggag tcccacaccg
     2281 tttccacgct gaaaggcgca ccgcccggct acgtgggtta tggcgaaggc ggcgtgttga
     2341 cagaagcggt acgccgccat ccctggagcg tggtattgct ggatgaaatt gaaaaagccc
     2401 atcacgacgt ccacgagctg ttcttccagg tgtttgataa gggggcgatg gaagatggcg
     2461 aaggtgtcca catcgatttc aaaaatacca cgctgttgtt gactactaac gcgggagcgg
     2521 aacttatcag ccagctctgt gaggatccga cgctgatgcc tgattcggca ggactaaaag
     2581 aggcgttaat gccggagtta cgtaaacact tcccggcggc tttcctgggg cgtgtgacgg
     2641 ttattcctta tttaccgctg gatgagaccg cgcgtggtgt tattgcccgc ctgcatcttg
     2701 gcagattgat ggatcggatg gcggagcagc atggcgtttc actggtgtat ggcgatgaat
     2761 tgattacgca tattgtgtcg cgctgcccga tgcacgaaac cggcgctcgt ttgctcatcg
     2821 gctatatcga acaacatatc ctcccacaac tgtcccgtta ctggttgcag gcgatgacag
     2881 agaaaacggc catcacgcaa atccgcattc gtgttgatga cgatgaacag gttatttttg
     2941 aaacggatta atggagcatc cttatggcaa aagaattaag tggcgcactg tgggtcagtc
     3001 gctttcctgg aagttcttcc actaatgatt tgcaggggac ttttcgtgca tcagtagata
     3061 attttttgcg cgcgcttggt aatgcgcgtg caagggtcag tatctctgta acatatcgcc
     3121 ctccc
//
LOCUS       DADKXM010000243         3123 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.246, whole genome shotgun sequence.
ACCESSION   DADKXM010000243 DADKXM010000000
VERSION     DADKXM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.246"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(15..893)
                     /locus_tag="JQR05_004000"
     CDS             complement(15..893)
                     /locus_tag="JQR05_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325968.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(890..3118)
                     /gene="crfC"
                     /locus_tag="JQR05_004001"
     CDS             complement(890..3118)
                     /gene="crfC"
                     /locus_tag="JQR05_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY5325969.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYLGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT          758 a          873 c          775 g          717 t
ORIGIN      
        1 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgaccat tgcccccacc
       61 aaacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc
      121 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
      181 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
      241 cgcgtggtcg gtgccgcatt gatactctct gccagatgtt gtgtcgcttt ctcgcgcaaa
      301 tcacccagca acaacaccac cgcttgcggg cttcccagac ggcaggcatc gccaaatgca
      361 ccccagcgca actcctccag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
      421 gccccagcac gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg
      481 cctcgttgcg ttaacgcctt ttccttccag cgacaacggg ctgaaaacca ctcatacaag
      541 gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc
      601 agcagcgcca gatgctgcgc gatctgcgtc agcgccagat gaccgggatc cagcatcgac
      661 gccagttttt ccaccattgt cagtcgcagg ctaagcggcg catttaacat gccgccgttc
      721 gtccatgtct gaagctgcac acgcgaaaga agttcctgca tcagtcgttc acgaaactgc
      781 tgctgatggg cttgaggatg gcgggcatca tcgccctgcg ccagatccac taaaaagcgc
      841 ggatgaaccg actccagcac gcgaccgggg ccgtccagta acgtcttggt caatatcgtt
      901 ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg agcgtcggtg taaatccagc
      961 tgtgagtcat cacagtctgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
     1021 gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttctgtcaaa catgaagcga
     1081 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
     1141 ctttagccat ttgttggcaa tagagatcga cttctgcgct taatgctcgt tgcaacacac
     1201 tgtaatcaac cgtttctgtc ggggatttct catttccccg tccccagtcg ggctgattca
     1261 accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt
     1321 cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actacgccgt
     1381 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
     1441 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg
     1501 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
     1561 agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaaggcg gctaactgcg
     1621 cattcaccgc atcttgctgt tgacgcagaa agtggttggc ggaggtcagg gccagctcga
     1681 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
     1741 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
     1801 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc
     1861 gcagagcata caatgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg
     1921 cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca
     1981 gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt
     2041 gttgctctgg cgctggtaac ttaccgttgt tggctaactc atagcgcgcc cgattcgcca
     2101 ggtagcccca catcgacgac accgggaata tctgctgtgg cgtaatacag cctttcatca
     2161 gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat
     2221 cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac
     2281 ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg
     2341 aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg
     2401 gcccgggggt atccagtaac gtcaactgac cgagataact ttccagcccc gccagatgga
     2461 caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat
     2521 cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa
     2581 taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa
     2641 gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac
     2701 gcaggcgctg ttgtaattgt tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca
     2761 gtaccggttc cttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc
     2821 gattacgatt aggcagaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg
     2881 ctttcatggt accgacaatt gccagcacca tttccagtcg ggaaatttta cgcaactcat
     2941 tattcagcat cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg
     3001 gcgcgtcgtc tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag
     3061 aaagctgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag
     3121 aaa
//
LOCUS       DADKXM010000244         3043 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.247, whole genome shotgun sequence.
ACCESSION   DADKXM010000244 DADKXM010000000
VERSION     DADKXM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.247"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            5..537
                     /locus_tag="JQR05_004002"
                     /pseudo
     CDS             5..537
                     /locus_tag="JQR05_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            544..777
                     /gene="mbeD"
                     /locus_tag="JQR05_004003"
     CDS             544..777
                     /gene="mbeD"
                     /locus_tag="JQR05_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY5325970.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(1322..1789)
                     /locus_tag="JQR05_004004"
     CDS             complement(1322..1789)
                     /locus_tag="JQR05_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325971.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(2858..>3043)
                     /locus_tag="JQR05_004005"
     CDS             complement(2858..>3043)
                     /locus_tag="JQR05_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY5325972.1"
                     /translation="KQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHGD
                     ELYRSCLARFGDDGENL"
BASE COUNT          735 a          685 c          854 g          769 t
ORIGIN      
        1 ttctatgagc aatcttttac agacgggcgc ggagttcgag aaaaagctga aagagagaga
       61 gccgaatcta cagagaaaat gctgaacgac gagttcagga agctcgaaga atctgtaagc
      121 aaggctgtga catcaaacga gacgaaaatc aaagacgcta tagccctgtt cacagcttcg
      181 acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg
      241 aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat ggtcattttc
      301 ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggatgataca gacgaacctg
      361 gaagaaatca ggaagcagaa cgagagtctg gagaagctga acgcgaagac atggggcgtg
      421 cgttatcacg aggggagcaa cggacgtttt ctggtgttgc ccgaggggat gaaagccgaa
      481 acgaactgga cgatggaaaa cgggaaacag aatgccgtaa ggctggtaca ggagtaacag
      541 agcatgacgg agctggaaag acatttgctg aacgcattag agcaactgca acaggactat
      601 atgcagcggc tgaacgaatg ggagagcgcc ttcgtggaat tgcagaaaat gttttcgctt
      661 acgcaacggg acaacgcgat gctgaacgag cgggtcatgc agttgagtca gcaggtgcag
      721 cacttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag gagataaggg
      781 acgaacggct gatacaggaa tgcgaacatg cgttatccct ggagcgtgag cgccagctgg
      841 aaatacagga acggacactg gatggccctt cgctgggatg gtaaccagag acaggctaaa
      901 atgttggccg cgcgtttccg gtgcattttt tgggataatg gttagttggg ggcaccggga
      961 acgcgcttca gtcagacctt gctgaaaaca caggaacgcg ttaaaacgca tacgggcgcg
     1021 attctggtgt gtcttttgtt gctgtgtttt gcctctattg ccagatcgcg ctcattcccc
     1081 tgaacttctg ttttcccgct caggtccccg tcttcttcgc ttccgaaagc atgaaaaaaa
     1141 tccgtttcag ctgtctctgg ctggcaattc ctgcgtgatt cacatggctg catagctatg
     1201 catgtggtta aaatttaccg ggtgcgatcg cggcagtttt tcggggggtt tgttgccatt
     1261 tttacctgtc tgctgccgtg atcgcaccga acgcgtttca gcggtgcgta caattaagat
     1321 attacgttaa attgtgaggg aatggtcccc atatactgac tttatcatag ggatagggtt
     1381 ggtagtgggt cgattgggtt tcggcattgt ttccgttaat gccaattgca tttttgtatt
     1441 tgatgcaaca gaaggcgtga gattctcctg ggcctggaaa ggataagtgt ttagaattga
     1501 tggcaaaata tcttccgtcc gatttttgaa gtagttgttg tttggctgtc tcccaaaggg
     1561 tgctgttaca aacttcttga atttgtagtt tttgcagggc tcttattact gcgttgtcat
     1621 gttccaattc tttaggggag tgtattattc tctcttttaa taagctgttt accacagggt
     1681 ttagttgttg tctaaagcac gcagataatg ccattgctac acaattttgt tcccgaggaa
     1741 gcatgtttgg cggggtatgc cgtgtttgat gtgatatgtc tattctcata gaaggtctct
     1801 ggcttatagc atcaacaaat acggctataa aacaaaattt tcatagagtg attcaagatc
     1861 ttcttgagat ccttttttgc ggcgcgtaat ctgctgcctg caaacaaaaa aaccaccgct
     1921 accaacggtg gtttgtttgc cggaatctag agctaccaac tctttcttcc gaaggtaact
     1981 ggcttggtgg agcacagata ccaaatgctg tccttctagt gtagccgcag ttaggccacc
     2041 acttcaagaa ctcttaatat ctcaatccac cttgtccagt taccaatggc tgctgccagt
     2101 ggcgttaagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2161 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2221 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2281 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2341 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2401 cggtttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccat cggcgcggcc
     2461 ttcttcctgt tcctggtctt ttgctcacat gttctttccg gcgttatccc ctgattctgt
     2521 ggataacagc atttcgtttt tgagtgagta gataccgctc gccgcagtcg aacgaccgag
     2581 cgtagcgagt cagtgagcga ggaagcggaa aagcgcttgg acgtgcattt tctccttacg
     2641 catctgtgcg gcatttcaca cccggtatgg cgcacttttc atacaatccg cactgatgcc
     2701 gcatggttaa gccattatac actccgctat cgctacgtga ctgggtcatg gctacgccct
     2761 gacacccgct aaaacctgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc
     2821 acagacaagc tgtgaccgtc tccgggagct gcatatgtca gaggttttca ccgtcatccc
     2881 cgaaacgtgc gagacagctg cggtaaagct catcgccgtg gtcgtgaagc gattcacaga
     2941 tgtctgcctg ttcatccgcg tccagttcgt tgagcttctc cagcagcgtt aatgtctggc
     3001 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg ttt
//
LOCUS       DADKXM010000245         2965 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.248, whole genome shotgun sequence.
ACCESSION   DADKXM010000245 DADKXM010000000
VERSION     DADKXM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.248"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(370..1167)
                     /locus_tag="JQR05_004006"
     CDS             complement(370..1167)
                     /locus_tag="JQR05_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5325973.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHDIHRVKPLMPALCRIRQGNKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1177..1728)
                     /gene="ybcL"
                     /locus_tag="JQR05_004007"
     CDS             complement(1177..1728)
                     /gene="ybcL"
                     /locus_tag="JQR05_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY5325974.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKISVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1897..2229)
                     /gene="emrE"
                     /locus_tag="JQR05_004008"
     CDS             complement(1897..2229)
                     /gene="emrE"
                     /locus_tag="JQR05_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY5325975.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2574..2888)
                     /gene="fliE"
                     /locus_tag="JQR05_004009"
     CDS             complement(2574..2888)
                     /gene="fliE"
                     /locus_tag="JQR05_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY5325976.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT          884 a          661 c          566 g          854 t
ORIGIN      
        1 ttctgggacg tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat
       61 tagatttaat aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt
      121 taattatccc ctcaacctct tgtaaatttc ccactcttga gctccacata gtccaaacgc
      181 cggatgcaaa aaaaatcaca cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa
      241 ttatatgtgc ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca
      301 ataggttgag tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat
      361 aaaatacttt tactctctgg acgcttttct tagctctctt ggtgttatct taaaacgatt
      421 atggaacctt tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct
      481 gatgggtttt actgtcgttt gtatagcaga caacgcatta tttaacctca cgtcgtccaa
      541 tatactttga aaacttattc cctcgcttgc cagacggcga tgcaatgtgg aaacagaaat
      601 gtagagatat ctcgctacct tgtttgctgt ccattttgtt ccaggttcgg atagcattag
      661 gttataacaa cgacttatta atgattgttt actatctgat aaaagtaaac aattaacatg
      721 atgtgctcct aatgaaagta gaacgcccat tgctaagtgt tcctgaattt gcgttgagaa
      781 gcctcgggaa atagatgttt ttagttgctc ccaacaatat attaactcgg ggttctgagg
      841 taagcagaaa cctgtcgtgt tacgaatttg atcagttatc gcataaaatt tttgaaactt
      901 ttcaattaga tcaatgggat aataaagcat ttctgcaaga taaagacctg cttctggata
      961 attcacaata taaaattcat atccacaggg aaataatatt atctgattat tatcaacagt
     1021 tagactatgc gtctcccaat tgataacttt atttccctga cggatacgac ataaagctgg
     1081 cataagaggc ttaaccctgt gaatatcatg atgtttatgc atccgtattt cttcaatctt
     1141 taggttagac ttatcttttg ccagcatact cttaccctac tttatctcat aaactggtgt
     1201 tatctcagcg gttgcaattt tattggcatt aagcatataa ccaactaacg ctccgctgga
     1261 attagaatcc acagaaatct tatcagtttt tagagcccat actttaaact ggtaatgatg
     1321 gggtttatct cctttgggag gacatgctcc accaaatcct gcataaccaa aatcatttcg
     1381 gccttgaaca gcaccagtcg gaagttttgt accatcacgt cttcctgcat cagtgggcaa
     1441 atatgttaca gttgctggaa tattagcaac agtccagtgc caccatccac tgcctgtagg
     1501 agcatctgga tcatacacag ttacggcaaa gcttttggtg ccttcaggag caccagacca
     1561 ggttaatgag ggcgatgtat taccaccttc acacccaaat ccagaaaata catgagacgt
     1621 tgtaagttgc tctcctgttt ttatttcatt actagtgacc tgaaatgctg cggcctgcgc
     1681 agaaaatgtt atgaatgcca atacacttga aacgataagt tttttcataa aaacctcatt
     1741 gttgtgacct atcgttattt tatttgatgt gccttgatct cattatgcat aaaggcgcaa
     1801 tgatcgcgca aatgcaatca taattataaa ggtgccccga acattagcca taatatgcac
     1861 aaatcagatt cctgttatct caaggataat tattttttaa tgcggtgcac ttcgtgacaa
     1921 caagttaatg actaacacac cggcacaaat caacatcatg cctatgatag ctggcaggtc
     1981 cagtcgttga ccaaaaaatc cccaggaaag caaactaatc agaacgatac cgactcctga
     2041 ccagatagca taagcaatcc ctgtaggtat ataagccagc gtctgagcta ataaccagaa
     2101 tgatgcacaa tagcaaataa ttgtgccaac agagggccat aaccgtgtaa acccctctga
     2161 aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcgccaccaa gataaatata
     2221 agggttcata gcatattctt tcctgttcat actggagaga attgtactac agtttgaaca
     2281 caactcaccg ttttcatcct ggtgtatgtg tatccaaggg gctctggcgt ggcaagagat
     2341 aaaggcagga acaataatga tcagaaggaa ccccaggcta acgaatgagt tcactcttct
     2401 tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg gatcgtggat ggcagaaaac
     2461 aaccaggatc aggtatccat tttaaaccag aaattgagtg agtttgcccc atccatgccc
     2521 cacgctgtgg gatcggatat aattaaacaa gcatagtcaa caggttacac aacctacacc
     2581 tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
     2641 tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc cacgccgggt
     2701 tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg tttgtgtatc gcttatgcga
     2761 tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag tgattcctgc
     2821 gcacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc
     2881 gctgacattt tcgtctcccg gataatttct ggtaacaaag cctaccagta agtcaataag
     2941 acaaaggcgc taaatagcaa caaaa
//
LOCUS       DADKXM010000246         2936 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.249, whole genome shotgun sequence.
ACCESSION   DADKXM010000246 DADKXM010000000
VERSION     DADKXM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.249"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            5..535
                     /locus_tag="JQR05_004010"
     CDS             5..535
                     /locus_tag="JQR05_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY5325977.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            542..775
                     /gene="mbeD"
                     /locus_tag="JQR05_004011"
     CDS             542..775
                     /gene="mbeD"
                     /locus_tag="JQR05_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY5325978.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFGELQKMFSLTQRDN
                     AMLNGRVMKLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(1350..2468)
                     /locus_tag="JQR05_004012"
     CDS             complement(1350..2468)
                     /locus_tag="JQR05_004012"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY5325979.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIANHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2586..2675)
                     /locus_tag="JQR05_004013"
     CDS             complement(2586..2675)
                     /locus_tag="JQR05_004013"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325980.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
BASE COUNT          918 a          517 c          668 g          833 t
ORIGIN      
        1 ttttatgagc aatcttttac agacgggtgc ggagttcgag aaaaagctga aagagagagc
       61 cgaatctaca gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag
      121 ggaactgacc tcaaacgaat ctttaatcag gaacgctatc aacgaccata catcagccct
      181 gaaagaactt ctggagaggt atcagaaaac gaccgtcgat acgatggacg cacactggaa
      241 aaccgtactg aaaatgagcg tgaaacgctg gctgtggctg attatcgtct cagtactgat
      301 gtttgccacg accgggagcc tgctgtggta ccagggcatg aagataaacg cgaacatgaa
      361 tattctccgc gagcagaaag agagtctgga gaagctgaac gcgaagacat ggggcgtgcg
      421 ttatcacgag gacagcaacg gacgttttct ggtgttgccg aaggggatga aagccgaaac
      481 gaactggacg aaggacaacg gcaaactgaa cgcagtgaga ctggtacagg agtgacagag
      541 catgacggag ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat
      601 gcagcggctg aacgaatggg agagcgcctt cggggaattg cagaaaatgt tttcgcttac
      661 gcaacgggac aacgcgatgc tgaacgggcg ggtcatgaag ttgagtcagc aggtgcagca
      721 cttgagcgag cggacagaac gcttgagcca gttatacagc gagaacagga gataagggat
      781 gaacggctga tgcaggaacg cgaacatgag ttatctctgg aacgggagcg ccagccggaa
      841 attcaggaac ggacactgga tggcccttcg ctgggatggt aaccagagac aggctaaaat
      901 gttggccgcg cgtttccggt gcattttttg ggataatggt tagttggggg caccgggaac
      961 gcgcttcagt cagaccttgc tgaaaacaca ggaacgcgtt aaaacgcata cgggcgcgat
     1021 tctggtgtgt ctttgttgct gtgttttgcc gttattgcca gatcgcgctc attcccctga
     1081 atttctgttt ttccgctccg gttccgtctt ctgcctttcc gaaagtatga aaaaatccgt
     1141 ttcagcagtc tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt
     1201 ggttaaaatt tatcgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac
     1261 ctgtctgctg ccgtgatcgc accgaacgcg ttttggcggt gcgtacaatg agatgttatg
     1321 gtaaattcaa gtcttagatt ttatgatgtt taattagctt tttttgcgtt tataatgttc
     1381 atctttttta ttaaaattga ttcaatcttt gatgtgggta ttaatatttt ctttgacaaa
     1441 tagaaagtta aaaaaaacac cattaatagt ataaataaat acccaaaaac agaagcgtta
     1501 ttaatttcaa ttattgatgt taatctgtat attactacat gctgaatcaa catggatatg
     1561 tagatgcatt tagcatatga gttgaaaaaa tagaggttaa atttcttatt aattattaat
     1621 gctgctttgt aaatgataat gcttatgcac attaaaggta ttattgataa ctggtgattg
     1681 gctatgtttg ttttgaactc attaggccct attaataata ctgtcaatat acatataaca
     1741 accgatagaa taactgtggt gtttttttgt aatttatttt tgtatttaaa tgcaagcatc
     1801 ccaaccataa actcaggaag tcggcaagca aaaaaccaaa atgggaagtt taaaaatgat
     1861 gatgagatat ttgatattgg gaatgttgta attgttatca caaataaaaa aaatatgttg
     1921 cgtttaaatg taaaggtatt cataacgtga aaaactatcg gtgatatcac gtacagcata
     1981 ataacgcacc caataaacca ctcgcctgta aggaaaagaa aatcataaga gaaatgggcg
     2041 tttaatatac catcaagggc aaaaaatact ggaattattc tagtgtactg gcctcctgtt
     2101 gtcattattg atacgagagg gactgtatta attatctctt tatttaaata agacaataaa
     2161 attaagagta aagagatgaa aaagtaagtg ataatataag gagtaaggat ccttactcct
     2221 ttgtcaagga aataatcaat ggtattcttt ctatctagtg aaataaatgc caaatatcca
     2281 gatataaaaa aaaacaagct cacacctacc tggccaagaa cccctccata aaatgatggt
     2341 atgaaagata gcctggtttg gctaaatatg tagccataat gagatataat aacaaaaatg
     2401 caggctgtta ctcgcaatgt atcaattgac ggatctctat tagacataac ctttatttcg
     2461 ctgttcaaat tatcgccttt agttgatgat gtaaaattaa cgctataaat aagctacaca
     2521 ttctagagca gcgaaaggat gattcaagta tttttaataa taaaacgcat tgataatgtt
     2581 tatgttcaga tggtgctgat agctttgtca tgctgttcca ctgatgttga taacgacaga
     2641 gacttttgtc cttttcttgt tagatgctgc ctcagattcg gattatggcg ttaaaggggt
     2701 ttgacgctca accgaacggt ttcacacgtt aagagctttt tgtctgataa atcaaaatat
     2761 aatggacatt gaaaaacgcc ctcaaaacgg tgtcttttta atcgaagatt ggcacaaaac
     2821 ggacaccacc gtccattagt cgatttttta gacggctttt ctcgcctaac gtgagttttc
     2881 gttcggttga gcatcagacc ccttttatat tgtagatttc tatttttaga cagttg
//
LOCUS       DADKXM010000247         2876 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.250, whole genome shotgun sequence.
ACCESSION   DADKXM010000247 DADKXM010000000
VERSION     DADKXM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.250"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..289
                     /locus_tag="JQR05_004014"
     CDS             <1..289
                     /locus_tag="JQR05_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY5325981.1"
                     /translation="PRSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRP
                     ERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            529..789
                     /locus_tag="JQR05_004015"
     CDS             529..789
                     /locus_tag="JQR05_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY5325982.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1013..1087
                     /locus_tag="JQR05_004016"
     CDS             1013..1087
                     /locus_tag="JQR05_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY5325983.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1080..1937
                     /gene="repA"
                     /locus_tag="JQR05_004017"
     CDS             1080..1937
                     /gene="repA"
                     /locus_tag="JQR05_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY5325984.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YNRLATASP"
     gene            2637..2828
                     /locus_tag="JQR05_004018"
     CDS             2637..2828
                     /locus_tag="JQR05_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325985.1"
                     /translation="MAENGYGLAGLGVGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          737 a          744 c          735 g          660 t
ORIGIN      
        1 gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc atgttgatgt
       61 ggttatcgtt gagctgcgtt cgttcagtat tgttgcagca gtagagctgg atgatgccag
      121 ccatttacga ccggaacgca gacgccggga tattcttctg gaagaggttc tgaggcaggc
      181 tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga ccggagaatg
      241 gctgaataca acaggggctg atcagcagtc accggaacat cgtagctgac gccttcgcgt
      301 tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttcctcgctc ccggcgtttc
      361 cataactgaa aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt
      421 tgatttgtgc gccaacacag ctcttcgcca caattctcaa gtcgctgatt tcaaaaaact
      481 gtagtatcct ctgcgaaacg atccctgttt gagtattgag gaggcgagat gtcgcagaca
      541 gaaaatgcag tgacttcctc attgagtcaa aagcggtttg tgcgcagagg taagcctatg
      601 actgactctg agaaacaaat ggccgctgtt gcaagaaaac gtcttacaca caaagagata
      661 aaagtttttg tcaaaaatcc tctgaaagat ctcatggttg agtactgcga gagagagggg
      721 ataacacagg ctcagttcgt tgagaaaatc atcaaagatg aactgcagag actggatata
      781 ctaaagtaaa gactttactt tgtggcgtag catgctagat tactgatcgt ttaaggaatt
      841 ttatggctgg ccacgccata aggtggcagg gaactggttc tgatgtggat gtacaggagc
      901 cagaaaagca aaaaccccga taatcttctt taactttggc gagtcagaaa gattaccggg
      961 gcccacttaa accgtatagc caacaatcaa gctatgcggg gagtatagtt atatgcccgg
     1021 aaaagttcaa gacttctttc tgtgctcgct ccttctgcgc attgtaagtg caggatggtg
     1081 tgactgatct tcaacaaact tattaccgcc aggtaaagaa cccgaatccg gtgttcacac
     1141 cccgtgaagg tgcccggacg cttccgttct gcgggaaact gatggaaaag gcggtgggct
     1201 tcacctcccg ttttgatttc gccattcatg tggcacatgc ccgttcgctt ggtctgcgtc
     1261 ggcgcatgcc accggtacta cgtcgccggg ccattgatgc gctgctgcag gggctgtgtt
     1321 ttcactatga cccactggcc aaccgcgtcc agtgttccat caccacgctg gccattgagt
     1381 gcggactggc gacggagtct gctgccggaa aactctccat cacccgggcc acccgggccc
     1441 tgacgttcct tgcagagctg ggactgatta cctaccagac ggaatatgac ccgcttatcg
     1501 ggtgctacat tccgaccgat atcacgttca catctgcact gtttgctgcc ctcgatgtat
     1561 cagaggaggc agtggccgcc gcgcgccgca gtcgtgtgga atgggaaaac agacagcgca
     1621 aaaagcaggg gctggatacc ctgggtatgg atgaactgat ggcgaaagcc tggcgttttg
     1681 ttcgtgagcg tttccgcagt taccagacag agcttaagtc ccgtggaatg aagcgtgccc
     1741 gtgcgcgtcg tgatgcggac aggcagcgtc aggatattgt caccctggtg aaacggcaac
     1801 tgacgcgtga aatctcggaa gggcgcttca ctgccagtcg tgaggcggta aaacgtgaag
     1861 tggagcgtcg tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac aaccggctgg
     1921 ccacagcttc cccctgaaag tgacctcctc agaataatcc ggcacgcgcc ggaggcattc
     1981 gcacgcctga agcccgccag tgcacaaaaa atcagcgcta catacaaaaa acaacctcat
     2041 catccacctt ctggtgcatc cggtcccccc tgtttttgat acaaaacacg cctcacagac
     2101 ggggaatttt gcttatccac attaaactgc aagggacttc accataaggt tacaaccgtt
     2161 cacgtcataa agcgccagcc gtcagcccta cagggtgcga tgtatctttt aaacacctgt
     2221 ttatatctcc tttaaactac ttaattacat tcatttaaaa agaaaacctg ttcactgcct
     2281 gtcctttgga cagacagata tgcacctccc accgcaagcg gcgggccccg accgaagctg
     2341 gtttagttac aacatgcaaa cataaacccc taaaaaaccc aagtccgacg cagaactgaa
     2401 accacaacgc ccctcctcat aactgaaaag cgccgccgcc cccgcccgaa gggcgggaac
     2461 agagtcgctt ttaattatga atgttgtaac tacatcatca tcgctgtcag tcttctggct
     2521 ggacgtatcg agtacacgct cgtaagcggc cctcacggcc cgctaacgcg gagatacgcc
     2581 ccgacttcgg gtaaacccta gtcgggacca ctccgaccgc gcacagaagc tctatcatgg
     2641 ctgaaaacgg gtatggcttg gcagggctgg gggtgggtaa ggtgaaatct gtcaatcagt
     2701 accggctgac gccgggcttc ggcggtttta ctccggtatc acatgtaacc acagcgtgcc
     2761 gccttccatg ccgctggcgc ggcatcagaa taatacaagc agcatttaat gcatttgcaa
     2821 aagtgtgagt aataaagact tgttaagtgg tcgtttcagg gatatcgtta agactt
//
LOCUS       DADKXM010000248         2872 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.251, whole genome shotgun sequence.
ACCESSION   DADKXM010000248 DADKXM010000000
VERSION     DADKXM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.251"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            27..944
                     /gene="rbn"
                     /locus_tag="JQR05_004019"
     CDS             27..944
                     /gene="rbn"
                     /locus_tag="JQR05_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY5325986.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            1132..2351
                     /gene="elaD"
                     /locus_tag="JQR05_004020"
                     /pseudo
     CDS             1132..2351
                     /gene="elaD"
                     /locus_tag="JQR05_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          902 a          628 c          539 g          803 t
ORIGIN      
        1 aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact tcagccggtg
       61 tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga
      121 gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct
      181 ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat catctttttg
      241 gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt
      301 atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga
      361 ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc
      421 gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag
      481 aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg
      541 gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat ggaaggcaga
      601 ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg
      661 ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg
      721 aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac
      781 gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt acccacgtca
      841 gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg tcaattttcc
      901 cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa
      961 aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca cccacacagg
     1021 catattgagc gatttttgat atttattcat ctccttacgc actatattca gagcaataag
     1081 ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc catgatggtt
     1141 acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac atttgcagaa
     1201 aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt atccggagat
     1261 gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt tggaaaaaaa
     1321 gctgaagata ttctttacca tattgttaga aacccaacaa atgagaccct atcgattatc
     1381 cgacttatta aaaatgcctg tttaaaatta tataatttgg cacataccgc aaccaactcc
     1441 caccttaaac caactggtcc ggataattca gatgttctcc tgttcaaaaa gctattctcc
     1501 ccttcgaaat taatgacaat tatcggtaat gaaattcctc ttatatctga aaaacagtcg
     1561 ctttcaaagg tgcttttaaa tgatgagaat aatgaactga gtgatggtac aaacttctgg
     1621 gataaaaatc gtcaattaac cacagatgaa atagcttgct atcttcagaa gatcgccgct
     1681 aatgcaaaaa atactcaagt caattatcct actggtctct acgtccccta ctccaccaga
     1741 actcacctgg aagacgctct caatgaaaat attaagagcg atccatcatg gccgaatgaa
     1801 gtccagttat tccccataaa tactggcgga cactggatat tagtttcgct acagaaaaca
     1861 gtaaataaaa aaataataaa ctacaaataa aatgcgtcat attcaactca ttgcgtgcac
     1921 taggccatga taaagaaaat tcacttaagc gtgtcattaa tagttttaat tctgaactca
     1981 tgggagaaat gtcgaataat aatataaaag ttcatttaaa tgaatcagag ataatatttt
     2041 tacatgccga tcttcagcaa tacttaagcc aaagttgcgg tgcgtttgtg tgcatggcag
     2101 cccaggaagt gattgaacaa agggaaagca attctgacag cgccccctat acgttattaa
     2161 aaaactatgc tgacagattt aaaaaatatt cagcagaaga gcagtacgaa attgattttc
     2221 aacatcgact ggtaaacaga aattgttatt tagataaata tggcgatgca cgaatcaatg
     2281 cttcttatac acaattagaa ataaaacact cacaacccaa aaatagagca tccggcaaaa
     2341 gagtgagtta acatcattgt taatcaattc gaccagtcag taaatatgct gactggtcga
     2401 acataatttg ctaaaaagtc atcactgact gatgtcagtc ccaccatctg ccagttcaat
     2461 ctacgggtat ttccagcgta tcgcggcacg cgattgctgg tctgttgatg tagtggattg
     2521 tagttgctga cttttcttac cctcagattt ctgccccact tgtcaattca atctgccaat
     2581 aaataacata attatatttt tattcctcac agaaattcct ttttaaaaat agcgaaccca
     2641 ctgtaaaaaa cactttttgt atacctctac caagcgtaat aaaataaacg ctcataattc
     2701 attataaata ataatgaaat aacgcctttt attgataatt tacatatgaa aaaaattttc
     2761 cttttgttat attatttctg ttttcactcc ctctgattat ttttaccaac cctgggtcac
     2821 tgcattatcg ccaacgcccc gacacgtaag tcctgagcaa ttagaaaaaa ca
//
LOCUS       DADKXM010000249         2867 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.252, whole genome shotgun sequence.
ACCESSION   DADKXM010000249 DADKXM010000000
VERSION     DADKXM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.252"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            139..1320
                     /gene="nimT"
                     /locus_tag="JQR05_004021"
     CDS             139..1320
                     /gene="nimT"
                     /locus_tag="JQR05_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY5325987.1"
                     /translation="MTCSTSLSGKNRIVLIAGILIIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYMIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFSFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1375..1722
                     /locus_tag="JQR05_004022"
     CDS             1375..1722
                     /locus_tag="JQR05_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY5325988.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JQR05_004023"
     CDS             complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JQR05_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY5325989.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            2181..>2867
                     /gene="dgcP"
                     /locus_tag="JQR05_004024"
     CDS             2181..>2867
                     /gene="dgcP"
                     /locus_tag="JQR05_004024"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY5325990.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGD"
BASE COUNT          663 a          723 c          709 g          772 t
ORIGIN      
        1 tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg aacttagcga
       61 taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt
      121 tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc
      181 cttatcgctg gcattctgat aattgccaca acattacgcg tcacctttac cggcgcagcg
      241 ccgttactgg atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta
      301 accaccctgc cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggcgcga
      361 cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca
      421 attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg
      481 attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc
      541 gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct
      601 atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg
      661 tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagtca gcaacatgca
      721 aatttgagta cctcacgcgc cttacatact cggggtatct ggcgttcgcc acttgcctgg
      781 caggtaacat tgtttcttgg gatcaactca ctggtctatt acatgattat tggctggctt
      841 ccggcgatcc tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg
      901 ctgcaactag ccacagcagc tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa
      961 gaccagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc
     1021 tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcagttttgg ttccggcgca
     1081 acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca tcaggcggcg
     1141 gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgcta
     1201 atgggtaaaa tacacgatgc taacggtaac tggtccgtac cactgatggg tgttgcaata
     1261 ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa
     1321 tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag ggagaacaaa cacgatgaat
     1381 attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc
     1441 gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa
     1501 gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca
     1561 acctttcgcg agcaatatcg cgcagaactg gcgcaacacg agcaagaagg aaagcggctg
     1621 gcggacatcg ccaaaaaaca gccgctgacc ctgctctacg cagcaaaaaa caccacgcag
     1681 aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta cggcatccgg
     1741 cggttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat caatcgtttc
     1801 accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt
     1861 cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt
     1921 aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc aaggcagaac
     1981 aaaactgata attttcattt attgatctca catatttatc caagcttaga gtatcgcggt
     2041 atcgttttgt tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt
     2101 tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag cacttacccg
     2161 ccgtatcctg gtgttgttcc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg
     2221 ccaaagaaca gtcactggaa agtctggtcc gacagcttct ggagatgctg gaaatggtca
     2281 ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta
     2341 tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt
     2401 actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccctgacc
     2461 gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa
     2521 ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc
     2581 agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata
     2641 ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa tgctgcgctg attgcgcaat
     2701 cgtataccga ctcgttaacc gggctaccga atcggcgggc gatttttgaa aatctgacga
     2761 cgctgttttc cctcgcccgg catcttaacc ataagataat gatcgcgttt atcgatctgg
     2821 ataacttcaa attaatcaat gatcgttttg gtcataatag tggcgat
//
LOCUS       DADKXM010000250         2861 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.253, whole genome shotgun sequence.
ACCESSION   DADKXM010000250 DADKXM010000000
VERSION     DADKXM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.253"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            141..485
                     /gene="tdcR"
                     /locus_tag="JQR05_004025"
     CDS             141..485
                     /gene="tdcR"
                     /locus_tag="JQR05_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY5325991.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            741..1281
                     /locus_tag="JQR05_004026"
                     /pseudo
     CDS             741..1281
                     /locus_tag="JQR05_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..2482
                     /locus_tag="JQR05_004027"
                     /pseudo
     CDS             1303..2482
                     /locus_tag="JQR05_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         1001 a          520 c          483 g          857 t
ORIGIN      
        1 aaatgaacag gatatgtgcg accactcaca aattaacttt cattactttc cagggtatcg
       61 ttattacaat taaatacctt taaatatcaa caagttaaag cataaaaatc agcataaagc
      121 ctgattttta tcaaaaaaaa atgacgggga taaccatatt ttatggcgat aacatcattc
      181 gttatgtggt taatacaaaa aaggggctga gaccatattt caagcaatta ccggataatt
      241 accaggcaaa atttgagtta aatttaatga gcaagttctc aaattttatt ataaataaac
      301 cattttcagc gataaatact gcggcacgtc acattttttc acgctatttg ttggagaaca
      361 aacatttatt ttatcaatat tttaaaattt cgaatacagg tattgatcat ctcgaacaat
      421 tgattaacgt caactttttc tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc
      481 cataaattct tctcccctca taggcgacga atagcatttt gtgttgagga tcacaaaacg
      541 aataattgct ggtcgccgcg ataaggtcag acgaagtcaa caagggggat tttcactgtg
      601 ctttacatca gcgtaggaca cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag
      661 cgttttttga tagtgcgtaa gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca
      721 tgacgacaag gcaggcatca atgaaaggct tcccaattgc acatattttt cacccttcaa
      781 tcccgccaat gcacgcagtg gttaacaatc acaatagaaa tattgattat tggacggtaa
      841 aaagaaagtt tgcagaaatt gtctccacca atgacgttaa taaaatttac agtataagta
      901 atgaactgcg gagagtatta tctgcaatta ctgcattgaa tttctatcaa ggcgatgttc
      961 cttctgtcat gatccgaatc caaccggaaa atatgagtcc attcatcata gatatttcta
     1021 caggagaaca tgatgattat atcatacaaa cattagatgt aggcactttt gcaccttttg
     1081 gtgaacaatg tacttgctca gccgtcaata aaaaagagct ggaatgtatt aaggagacga
     1141 tttctaagta ttgcgcaaag ttcacccaaa aagaagccat cttaacaccc cctaggcact
     1201 ttaataaaac ctgcattact tcagattgtt ggcaaattct cttttttttc acccgatcat
     1261 tttaataatg atttttattg attatttata aaggataaaa taatgttccc tgtttcatca
     1321 attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc
     1381 cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct
     1441 attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt
     1501 gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt
     1561 cgtctggatt ttttaggtta taacgcccaa attattcaac gatcggacaa tacctgcgaa
     1621 cttaccatta atgaaccgtt aaaaaaccag gaaatatcta caggcaatat caatgttaat
     1681 tgcccattaa aagatattta caatgaaatc aagaggttga acgtaatctt tagttgtggg
     1741 actggagata tagttgatct atcctctctg gacattacgt aatgtcgatt tagattatta
     1801 tgatttcaca gataaacata tggctaatac tattctaaat cattttaaat tggattcaac
     1861 aaattttact aatgccaaca tgtttcaggt taattttgtt agttcaacac aaaacgccac
     1921 aatctcctgg gattatttac taaaaataac gcctgtttta ataagcatta gcgatatgta
     1981 ttctgaagaa aaaatcaagt ttgtcgaaag ttgtttaaat gagcttggag acattaccga
     2041 agaacaatta aaaattatga gatttgcaat tataaaatct ataccaaggg caactcttac
     2101 agataaatta gaaaatgaat taacaaaaga aatatataaa agctcatcga aaatcaacaa
     2161 ttgcttgaac agaattaaat taccagagat gaaagaattc tcatcaaaaa aaaatatatg
     2221 attacatcga tacaatcatt gaagattatg aaaatatcaa agaaaatgct tatctggtcg
     2281 ccccccaaat taattatact atggatttaa acatagaaga ctctagctca gaagagttaa
     2341 taccctcgag aaagacgaaa attctccgga caatggcttt gaggtcgggg aatataacac
     2401 atatgaagca tataactcag agaatcaata ttttaccaga gaggactata cgtataatta
     2461 cgacctttta aatgcaatat agacttaata acttcttaca aagtctatta ttaataccgt
     2521 agatattcat cttaccctgt gcaccaaaag caaaaactta tcgacgcggg tttgaaactg
     2581 atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag gcctacgcca tctctgcaat
     2641 atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa
     2701 taaagcgcac tcaacaatct gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa
     2761 ctgaccgata agccgctttc ttttgggtat agtgtcgtgg acagtcattc atctttctgc
     2821 ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt a
//
LOCUS       DADKXM010000251         2783 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.254, whole genome shotgun sequence.
ACCESSION   DADKXM010000251 DADKXM010000000
VERSION     DADKXM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.254"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JQR05_004028"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JQR05_004028"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY5325992.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(1698..>2783)
                     /locus_tag="JQR05_004029"
     CDS             complement(1698..>2783)
                     /locus_tag="JQR05_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY5325993.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          736 a          651 c          672 g          724 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccacttcagg cgtgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaa
//
LOCUS       DADKXM010000252         2780 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.255, whole genome shotgun sequence.
ACCESSION   DADKXM010000252 DADKXM010000000
VERSION     DADKXM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.255"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..484
                     /locus_tag="JQR05_004030"
     CDS             <1..484
                     /locus_tag="JQR05_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HAY5325994.1"
                     /translation="KTAPFSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQA
                     TKAAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLK
                     ERPFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA
                     "
     gene            612..1136
                     /gene="yfdR"
                     /locus_tag="JQR05_004031"
     CDS             612..1136
                     /gene="yfdR"
                     /locus_tag="JQR05_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY5325995.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPTPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFKELTES"
     gene            1245..2111
                     /locus_tag="JQR05_004032"
     CDS             1245..2111
                     /locus_tag="JQR05_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325996.1"
                     /translation="MNQNPFSFYDFLGYLIPGGFFILLMYFCGLTFDLDIVIDLTELL
                     RGQSQIFGILNYGSIVIISYIAGHFISITSAFFIEKYMNEELQYPSEYLFNKLSDDKK
                     NVLTPSSDKQNEDTKRKIKYCIIKVMLFPIIPWDYATQKLCYSQSLPFQLANTTWLMI
                     KEGYEKKFRIDDKLLNIRTGLHDDLFRLAYHYVYEFSNQHQTKIQNYVALYGFCRNIC
                     LVFVITFWISFLTLIYRAIEGGAVSYISISTLLSFFFVYVFYVGFVKFYRRYTLEVLM
                     AFAVLQSKDAIN"
     gene            2152..2451
                     /locus_tag="JQR05_004033"
     CDS             2152..2451
                     /locus_tag="JQR05_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator protein PrtN"
                     /protein_id="HAY5325997.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKAKAKAKAN
                     ACKLPVPTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
BASE COUNT          873 a          546 c          553 g          808 t
ORIGIN      
        1 aaagacagca ccgttctctg ctctgttgtc tgttaatggc gagcgtcatt cccagaagtc
       61 actggcagaa tggattgaag actgggccga ctaccttgtg ggctttgatg ctaatggtga
      121 cgctattcag gcaacaaaag cggctgcggc tgtccgtaaa atcacgattg aagcaaacca
      181 gaccgctgat tttgaagata atgacttcag cggcaaacgc tccctgatgg agtctgtcga
      241 agcgaaaacc aaagatatta tgccagtggc atttgaattt aaatgcgttc cgtttgaagg
      301 tctgaaagaa cgtccattta aattacgcct cagcattatc actggcgatc gtcctgtact
      361 ggttctgcgc attattcagc tggaggcggt gcaggaagaa atggctaacg aatttcgtga
      421 tctgcttgtt gagaaattca aagacagcaa agtagaaacc tttattggta ctttcaccgc
      481 ctgatttcat tactgcaaat gcccctgcgg gggcatttat ggaaacgtaa ttgactcaat
      541 aatcgccgga tggtgagggc ttccttttac cagaattcag cgtggtgcag cacatatacg
      601 cggagaacaa aatgtcattt attaaaactt tttccgggaa gcatttttat tatgacagga
      661 taaataaaga cgacatcgtg attaacgata tcgcggtttc cctttcaaat atctgtcgct
      721 ttgccggtca tctttcacac ttctacagtg tcgcccaaca tgcggtgctt tgcagccagc
      781 tggtgccgca ggaatttgct tttgaagcgt taatgcatga tgcaacagaa gcgtattgcc
      841 aggacatccc cacgccgctg aaacgccttc ttcctgacta taaacggatg gaagaaaaaa
      901 tagacgccgt aatccgtgag aaatacgggt tacccacggt tatgagcacg cctgtgaaat
      961 atgccgatct catcatgctg gcaaccgaac gccgcgatct cgggcttgat gatggctctt
     1021 tctggcctgt actggaaggt atcccggcaa cagagatgtt caaagttatt ccactgtcgc
     1081 caggccatgc ctatgggatg tttatggaac gctttaaaga gttaactgag tcgtaaaaat
     1141 cagcacgtac gaattcaaac tctgccataa aagaacatat aagtaattta ttaacatata
     1201 gatataggtt atattacaaa ttgaaaaatt attggagagc aacaatgaat caaaatccat
     1261 tctcattcta tgactttctt ggatatttaa taccaggtgg tttttttatc ctattaatgt
     1321 atttctgtgg tttgacattc gatctagata ttgttattga cttaactgaa ttgctcagag
     1381 gtcaaagtca aatctttggc attttgaact atggttcaat agtaatcata tcttacatag
     1441 ctggacactt tatatctatc acatccgcat tttttattga aaaatatatg aacgaagaat
     1501 tgcaatatcc gtcagaatat ctatttaata agttatcaga tgacaaaaaa aacgtcttaa
     1561 caccatcaag tgacaaacag aacgaagaca caaagagaaa aataaaatac tgcataatca
     1621 aagtaatgtt attcccgata ataccatggg attacgccac tcaaaaacta tgttattcac
     1681 aatcattgcc atttcaatta gctaatacca catggctaat gatcaaagaa ggttatgaaa
     1741 aaaaattcag aatagacgat aaattattaa acatcaggac aggtcttcat gatgatcttt
     1801 tcagattggc ttatcattac gtctatgaat tttctaatca acatcaaaca aaaattcaaa
     1861 actatgttgc attatatggt ttttgcagaa atatttgttt agtatttgta attacatttt
     1921 ggatatcatt tctaacatta atttatcgtg ctatagaagg tggggcggtg tcttatattt
     1981 ctatttcgac gctacttagc tttttcttcg tctatgtttt ttatgttgga ttcgttaagt
     2041 tttatagacg atatacttta gaggtattaa tggcatttgc tgtacttcag agcaaagatg
     2101 ctattaatta atatattacc ctcgggtgca gccgggataa tggagaaatt tatgaacacc
     2161 ttgtttttac tgatggctga attcaatacc tcaaacattg aactctcagc agttagccaa
     2221 aagtactttg gtatgagtcc agccacagca gaagcaaaag caaaagcaaa agcaaaagca
     2281 aacgcttgta agttgcccgt tccaacatat cgcatcggca catcacaaaa agcaaaacgt
     2341 tgcatcaata ttcaggatct tgcggaatac atagacaaaa gacgagaaga aggacgtatc
     2401 gagtgggaac aggtcagaac aggcaaacag aagggcaaag aacatcacta aagaaaaaac
     2461 ccgcctaaag gcgggttttc aaaaagcacc agctatgatc atgctgcttt gcgacgacga
     2521 agcttaccct gctgctcttt accagagaca gtagcgtgag tgaacgcatt aggagcagcc
     2581 ttcatcagaa cttcaaagca gcccccatac ctgcgaatgc tttcattgtg tcgaacttaa
     2641 gctgtggctt ggttgctttt tgatcttcca tagaaaactc cagaagttat accgaaacaa
     2701 ttcctgttgt ttactcatca tcaatagatg atacgcaata tttattttta aatttaaggt
     2761 tctttggcgt aacttcatca
//
LOCUS       DADKXM010000253         2743 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.256, whole genome shotgun sequence.
ACCESSION   DADKXM010000253 DADKXM010000000
VERSION     DADKXM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.256"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(101..472)
                     /locus_tag="JQR05_004034"
                     /pseudo
     CDS             complement(101..472)
                     /locus_tag="JQR05_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(469..954)
                     /locus_tag="JQR05_004035"
     CDS             complement(469..954)
                     /locus_tag="JQR05_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5325998.1"
                     /translation="MGRPSFNEAWSAFGKVNFPVADVGRIIGGNVGQNISGGYFENAC
                     PIRMSYVLNATGFPISRSSPYATVSGSDKKFYIYRVNDMIEHLTQIMGKPDLVINNPT
                     PGDFTGKKGIIVVKGHGWSNARGHVTLWDGSVCSDQCHLLNDPDNGTFVPEVGILWVL
                     P"
     gene            complement(1007..2503)
                     /gene="tssC"
                     /locus_tag="JQR05_004036"
                     /pseudo
     CDS             complement(1007..2503)
                     /gene="tssC"
                     /locus_tag="JQR05_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181815.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            complement(2546..>2743)
                     /locus_tag="JQR05_004037"
     CDS             complement(2546..>2743)
                     /locus_tag="JQR05_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY5325999.1"
                     /translation="DALNQLLEARTQLANLQTYMDGKAGAENLINKLLQDPTLLKALA
                     SAPKPVAQQESVLSGETGTAD"
BASE COUNT          760 a          653 c          560 g          770 t
ORIGIN      
        1 tttatttaaa ttaattttgg tttatccgct tacaaataaa ccattacttt acaacactct
       61 tccacatatg gaatcggcag aacaaaggat tgaatatatt tcatttctta acactcctgg
      121 aaatcaactc atccaattca tttccatgaa tgaaatctat acatttcata gtgctgaagt
      181 ctgatgctat agaaccatca tattttaatg atacatattt tctcgctatc gcatctattt
      241 cacgataaat ttctacaggt tgctgaccat attcaagcta agcactagct gtagctctgg
      301 catcatcctg aacggcatca tttttataca ccaatgacaa acaatgactc aaagcccaat
      361 tgcgtaagag ctctgactga gaatattttt cagtcaatgc ttcctgagca taagataatt
      421 gacatatctg tagcccacag aggccccaaa atattaattt cagccatttc atggtagtac
      481 ccacaggatt ccgacttcag gaacaaatgt tccattatct ggatcattta gtagatgaca
      541 ttgatctgaa caaacgctac catcccataa tgttacatgt ccccgagcat tgctccagcc
      601 atggcctttt acaacaatga tgcctttttt acctgtaaag tcaccaggag ttggattatt
      661 aatgacaaga tctggcttcc ccataatctg ggtcagatgt tcaatcatat cattcacacg
      721 ataaatataa aatttcttat cagaaccact aaccgttgca tagggagaac tacgggatat
      781 cggaaatccc gtagcattca aaacataact catacgaatt ggacaagcat tttcaaaata
      841 gccacctgaa atattttgcc caacatttcc gccaattata cgaccgacat cagcaacggg
      901 aaaattcact tttccaaatg ccgaccatgc ctcattaaat gaaggtctac ccattttctt
      961 taccctctaa aaaatctgca tcacaagctt tatgaaatat aataaatcag gcattaccct
     1021 gttttaccga cggcagtttt gtgaccagac gcagtgatcc tgttaaccct tcaagctgaa
     1081 aatgcgggcg caggaagaat ttagcatcgt aataacccgg attaccttcc acctcttcca
     1141 ccactacttc agcagctgcc agtgggcgac gagctttggt ttcctgtgag gagttcaccg
     1201 gatcggcatc gacgtagttc atgatccact cattgagcca gcgctgcata tcttcacgct
     1261 ctttgaacga gccgatctta tctcgcacga tacatttgag gaagtgcgca aaccgtgaac
     1321 atgcgaaaag atatggcaaa cgagctgaaa gattggcgtt ggccgtagcg tccggatcgt
     1381 aatactcctg cggtttttgt agagactgcg caccaataaa ggcggcgtaa tcagagtttt
     1441 tgcggtggat aagtgggata aaaccatttt tcgccagttc agcttcacga cggtcagaga
     1501 tggcaatttc tgtcgggcat ttcatatcca cgccgccgtc atcggtcggg aaggtatgac
     1561 agggcagatt ttccaccgcg ccaccagatt ccacaccacg gatcaacgta caccaaccat
     1621 aatgtttgaa agaacggttg atatttacac ccatcgcata ggcggcattg ctccagacat
     1681 atttgctgtg atctgcgcca tccgcatctt cttcaaaatc aaactcatcg acagggtttg
     1741 ttttcgcacc ataaggcaaa cgggagagaa aacgtggcat cgtcaggcca atgtagcgag
     1801 aatcctcact ttttcgcagc gagttccacg gtgcgtattc gaggttttgg gtcacgattt
     1861 tggtcaaatc acgcgggtta gccagttcct gccaggagtc catctgcaac actgacggag
     1921 aggccccggc aataaacggc gcatgggctg aggctgctac tttggcaatg gaaccgagca
     1981 gatccacatc cggtggcgtg tgatcaaaat aataatccgc aataatacaa ccgtaaggtt
     2041 ctccacctaa ctgtccatat tcagcttcat aaagcttttt gaacataggg ctttgatccc
     2101 acgcgatccc tttgtaacgc ttcatatttt tccgcaattc atcctttgaa agattcataa
     2161 agcggatttt taatttttca tcggtttcag tgttgtaaac caaatgttcc atcccgcgcc
     2221 aggaggacta cagtttctga aattccggat gactcaggat gagtttgatc tgctcagtta
     2281 atttgaaatc aatctgctca ataatcgcag caatactttt ataagcgtcg tcactgacgg
     2341 tgacggtatt tgtcagagcc tgttgggcca gcgtctgaac tgctgcctca acagcctttg
     2401 ctgcctgagg tgtcttggga cggaaggctt tatccaggag cgaatcaaat tcagtgtcct
     2461 gataggcagt ggtttgcgca gcagagagat cgccagtcgc cagaagagta tcgtttacca
     2521 tgaaagttcc ttaataaaat cgcagttagt ctgctgttcc cgtttcacct gataagacac
     2581 tttcctgttg agcgacaggt tttggcgcac tggccagagc tttcagtaac gtagggtctt
     2641 gtaatagttt gttgataaga ttttctgctc ccgctttacc atccatatac gtttgtaggt
     2701 tggcaagctg agtacgcgct tccagcagct ggttcagggc atc
//
LOCUS       DADKXM010000254         2667 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.257, whole genome shotgun sequence.
ACCESSION   DADKXM010000254 DADKXM010000000
VERSION     DADKXM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.257"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..548
                     /gene="ynaI"
                     /locus_tag="JQR05_004038"
                     /pseudo
     CDS             1..548
                     /gene="ynaI"
                     /locus_tag="JQR05_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
     gene            complement(599..2212)
                     /locus_tag="JQR05_004039"
     CDS             complement(599..2212)
                     /locus_tag="JQR05_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5326000.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2549..>2667)
                     /locus_tag="JQR05_004040"
     CDS             complement(2549..>2667)
                     /locus_tag="JQR05_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5326001.1"
                     /translation="LDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          714 a          632 c          586 g          735 t
ORIGIN      
        1 atattctgag taatttcttt tccgggatca tgctctattt cgaccgtcct ttcagtattg
       61 gcgactggat ccgttcaccg gacagaaata tcgaaggtac agtagcggaa attggctggc
      121 gaattaccaa aattacgacc tttgataatc gtccattgta cgtaccgaac tcgctgtttt
      181 cgtcgatcag cgtagaaaac ccatgacgaa tgaccaaccg ccgcattacc acgaccattg
      241 gtttacgtta tgaggatgcg gcaaaagtgg gcgttattgt cgaagccgta cgtgagatgc
      301 tgaaaaatca cccggccatc gaccagcgac aaaccttact ggtttatttc aaccagtttg
      361 ctgactcgtc attgaatatt atggtttatt gctttaccaa aaccacggta tgggctgagt
      421 ggcttgctgc acagcaagac gtttatttga agattatcga tattgtacag tcacacggcg
      481 cggactttgc cttccccagc cagacgctgt atatggataa cattacaccg ccggaacagg
      541 gtcgctaaat cagacatcac tggcgttatc cacaagggta acgccagttc ccatcacatc
      601 aatgcttcac aatatacata gtccgactgt acgctacatc ttcagggtta ttaatgggat
      661 aacctttcag ccacggtttg attaatcgtc cattggtata ttgataaatt ggtgcaatcg
      721 gtgcttgctc catgaggatt ttttctgccg cgttgtaatc ggcattacgc gctttaacgg
      781 tattttccgt cgatgcctgg gccagaactt tgtcgtatgc cggattgtta aagcgtgaaa
      841 tatttcctga atgcattgac gttaataatg tcaggaaagt ggagggttca ttataatcac
      901 ccacccatga ggcgcggata acatcaaaat tgccggtgtt acggctatcg atataggttt
      961 tccattcctg attttgcaat ttaacatcta cgccaaggtt ctttttccac atcgatgcta
     1021 cagcaatcgc aattttttga tggttttctg aagtgttata caaaagcgtc agcttcagcg
     1081 gtttttgcgg accataacca gctgcgctca acaaagtttt tgcctgcgca ttcagttctt
     1141 cctgactcat ttgttcaaac ggcgaaggtt ccggcgtaaa tccggcggta acatctggta
     1201 taaaatgcca cgctggcttt tcgcccgtcc ccaacacttt ttcggtcatc aggcggcgat
     1261 ctatcgtcat acttaatgcc aggcgaacgc gctgatctgc cgtcggccct ttttgcgtgt
     1321 taaaggcata ataataggtc ccgagctgcg gtggcgtata aacctgcccc ggaatatcct
     1381 tcaacagctt ctgatacata tttttcggga aggattcggt gatatcaata tcccccgcga
     1441 ggtaacgctt agtggctgcg gattcctgat taattggcag gaaggtcact ttttgcagca
     1501 ccgttttggc gttatcccaa taatgggtat tcggtaccac gaccagtttt tcattgacta
     1561 cgcgctcttt aagaacataa gcgccattgc cgatcagatt tccgggtttc gtccactctt
     1621 taccgctttc tacgttggct ttttgcaccg ggaagaaggc aaagttagcg gttaaattca
     1681 caaaccacgg caacggttta tcaagctgaa ttttcaaagt atgggcatca actgcggtga
     1741 cgccaagctg atcaggcgta gctttaccat caataatcgc ctgtgcgttg ttgattcccg
     1801 ccagcgcggc aaaccatgca aatggcgaca atgtttttgg gtccaccaga cgttgccagc
     1861 tgtagacaaa atcttgcgcc gttaccggtg tgccatccgc ccattttgcg ttatcgcgca
     1921 gggtaaaagt ccagatacgg ttgtcattac ttttccattg agtcgcgacg ccggggacaa
     1981 tctccccttt ttcgttctga ttcaccagac cttcaaacag atcgcgaatg acctgaatct
     2041 ctggcaggcc cacggcttta gcgggatcca gcgacgcagg ctcatcttta atgtggcgca
     2101 ccagctcctg cttctctgcc agtactgtgc cgctcggaac ttctgcagca tacgaaagag
     2161 aaatgctgct gaccaacagc gcacaacacg taactgaaac agagtgcttc ataagtttcc
     2221 ctctaaccga cattaaaatt gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca
     2281 acttccatca aaaaagtgac aaaggaagca tcaaatgttt taagtatcaa tatatcaaat
     2341 cagatttagt gtaaataata gacgccaaaa tgcggcaaag cacatagaac accgaacact
     2401 acgttcgggc ttcgcacgtt caaaccagtt ttcggtatca taatcagcat ctgtcgccat
     2461 tgtacgaaga ataaaaaaca accaaaacgt tgaaaggaac tgagaatacg ttaagagaag
     2521 aattaaatta atgtatcagc aagtagtgct aaacagcacc tttatacttc agcctatcta
     2581 taaataatga gaatgccgag ccagcatgtc gacggtgtgg atagtacagg tgataaccgg
     2641 gtaaatctgg agtgaattta tccaaaa
//
LOCUS       DADKXM010000255         2644 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.258, whole genome shotgun sequence.
ACCESSION   DADKXM010000255 DADKXM010000000
VERSION     DADKXM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.258"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            335..1855
                     /locus_tag="JQR05_004041"
     CDS             335..1855
                     /locus_tag="JQR05_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY5326002.1"
                     /translation="MFLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLLSMVISIEI
                     SSIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNI
                     TVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKETVTNDSYS
                     HEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRG
                     QLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNS
                     IDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVG
                     THLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYP
                     SEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHN
                     MFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKTELIKQLQLFGDR"
     gene            complement(1924..2644)
                     /locus_tag="JQR05_004042"
                     /pseudo
     CDS             complement(1924..2644)
                     /locus_tag="JQR05_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="replicase"
BASE COUNT          890 a          474 c          465 g          815 t
ORIGIN      
        1 tcagtagcgg aacaggaggg acagctgatg gaaatggaag ccaccggagc acctcaaaag
       61 caccgtcatc cactaaatca gtaagttgga agcatcaccg cactagcaga cagttaatta
      121 aaaaagtata atccctctcc ggatttattg aataatggag aattatgctg ttttctatgt
      181 cttatttagt caaatgcgct tatcaatatt gtgataagct cccgagtcta aacttaactt
      241 actgatttta ggttgaggaa gaccggcatt tttgtagatc cacggcataa tatctattta
      301 ttcaacaacg ccccccaaaa aaaggttttt ataaatgttt ttaatttcaa tcattcaaat
      361 gttctcggtt atgattataa tgcttaatat aaaaataaaa gatgcattta gagcaattat
      421 tctgctttta cttctattac tatccatggt tatatctatt gaaataagca gcattatatc
      481 aataggtgag tacataccca cacttgcact agcaaatata tcagagacaa atagcgttgg
      541 ctggaaaata aaactactac cttttcttgt gttcttgtca tttatgattg catcattgat
      601 tttgaaaaga ttgataaaag aaaaaaaaat aagaaatata acggttgttg tttttttatt
      661 atcattgtta ataccaatat ctccaataaa tgcttttttt tcttcatcat atgaagttta
      721 ttcggaagca aaagaataca atatgtcacc acatgaaata agtgatataa agtcaaaata
      781 cacaaaagaa acagtaacaa acgacagtta ctcacatgaa ataataaatg gaaacggaaa
      841 aaatatttta gtaatattta ttgaaggtat gtcttcattg gtaatatcta aggagctaac
      901 cccgaatcta tatgaattta gaaaaaaatc aatatcattt aacaactatt acaaccatac
      961 cgcagctaca tttagaggtt taagaggaca gttaacctca tcatatcaaa tgaccggtgg
     1021 gtaccataaa gataattctg gccttggcca agcatctaaa gtagagatcg aacataaact
     1081 aaatacttac aattacataa caaaattacc aatcatactt gaggaaaatg gttaccatac
     1141 atattttcaa gcatctaatt caatagatga cccattgagt ttaatgctat caactttaaa
     1201 gtttaatcat atatacggaa gggaggacta taacaaaaag tcaggtgaac tcacagacaa
     1261 agagtctttc gatttattat ttaaaaaatt aaatgaggct aaacaacctt tcttctatgg
     1321 tgtttatact gtaggtacac accttggctt agatagccct gatgtaaagt tttatgatgg
     1381 gagaaatgaa tatttaaatc actttcatca attagaccat tggtttggtg attttatatc
     1441 tcattttaat aagacaagct tatcgaaaga tacaattgtc ataatcacaa gtgaccatgc
     1501 aagttaccca tcagaagatt acaaaaaaac attcaaaact gataatactt tttttgttga
     1561 tcagattcca ttaataatat ggagtaatgg tgttgagttc aaagacatta atgcacacgg
     1621 tttaaattca ctatctttaa cgccaacaat actagatatt cttgggatca aggagtcaca
     1681 caacatgttc ataggtgagt ctctatttga aaaagaaaaa aataaaaatg aactcaactg
     1741 tacatctgca ataggtgacc tgtatgtgaa aacgtgtaat ggaaaaatgg aaatcgagaa
     1801 aagaaaagaa aaaacagaat taataaaaca attgcaactt tttggtgata gataattaaa
     1861 aggcggtttt accccatgtt gattattagt ccacgtgggg ttagtttaaa atgataataa
     1921 aacttacttt ttttcattct tgagccgcct gtagaatgtg gctcgactaa cacctaaaga
     1981 ctcccattct ttttttaaag ttatatgaat taatattaaa actaccttta ggccgtcctc
     2041 ctgaattgct tagctttcct cctttacccc ctctctttct ttgaatttct gaacgggcgt
     2101 attttttaca tactcaataa aacgatcctc tgaaaaatta ttcgctgtcc attttgagat
     2161 gctttttgat attccaagaa cttcattttc acttaatggt gatacgaatt gtaaattgta
     2221 ggcacaggct cgttcatagc aggcttgtaa ccactgctca tactcaggcc acccctgacg
     2281 gatggcacga tatgcccact tgcgggtttt gtcgaacaga gtgcagttac ggcctaaacc
     2341 ataatcggcg gcaattttcc agtgactgtg gttcggattc ttgcaaatta gccccgaata
     2401 tcccgtgtca gctcccaatt ttctacgtag cgcattctct acggcagctg cgtagcgtaa
     2461 cggtttcatt tttccgtcag gtgctgtgcg tatagaggtt tccagcgcat aaagcaggtg
     2521 cgcgtgtccg ttttcggggt tggtgatggt cagcgtaggt gcgggagcgt tgcggtcgct
     2581 ccagtcaatg gctgcgccga tgcggtcaac atcaaatccc agccagaaca tggcgtgagg
     2641 ctga
//
LOCUS       DADKXM010000256         2567 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.259, whole genome shotgun sequence.
ACCESSION   DADKXM010000256 DADKXM010000000
VERSION     DADKXM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.259"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JQR05_004043"
     CDS             complement(<1..209)
                     /locus_tag="JQR05_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traK family protein"
                     /protein_id="HAY5326003.1"
                     /translation="MRKNNTAVIFGGLFFSCSVMAGAGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALT"
     gene            complement(196..762)
                     /gene="traE"
                     /locus_tag="JQR05_004044"
     CDS             complement(196..762)
                     /gene="traE"
                     /locus_tag="JQR05_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAY5326004.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGGNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSVDASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(784..1095)
                     /gene="traL"
                     /locus_tag="JQR05_004045"
     CDS             complement(784..1095)
                     /gene="traL"
                     /locus_tag="JQR05_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAY5326005.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(1110..1475)
                     /gene="traA"
                     /locus_tag="JQR05_004046"
     CDS             complement(1110..1475)
                     /gene="traA"
                     /locus_tag="JQR05_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAY5326006.1"
                     /translation="MNAILSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMASAGKGTDLMAKGQETVKATFGKDSSIVTWVILAEVLVGAVMYMMTKNVKFL
                     AGFAILSVFIAVGMAVVGL"
     gene            complement(1509..1736)
                     /gene="traY"
                     /locus_tag="JQR05_004047"
     CDS             complement(1509..1736)
                     /gene="traY"
                     /locus_tag="JQR05_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAY5326007.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            complement(1830..2516)
                     /locus_tag="JQR05_004048"
     CDS             complement(1830..2516)
                     /locus_tag="JQR05_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAY5326008.1"
                     /translation="MCALDPKERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENISFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIRPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYSVK"
BASE COUNT          765 a          612 c          510 g          680 t
ORIGIN      
        1 ttggtcagag caccatccag actgttcacg gcgataatcc ggtcaccggg aacggtaaac
       61 agattcgggc tggtattgct gatggcaata ctggcctgac caccgttcac cattggcacc
      121 acggtggggg ccagcgtacc ggctccggcc atcacgctgc aggaaaaaaa caggccgccg
      181 aatattactg ccgtattatt ttttctcatc gtctgtttcc ccgaaattat ccagccaggt
      241 caccccgttt tcccgcttca gaataaggat gtaatgtttg atatcagtga aaggttttga
      301 atcacctatc caggttttaa gcacaccacg aatttccaca cggccatact gaggccagac
      361 acgaacactg gtctggaaaa aggctgagtt cacgttatcg tttttaatac gcttcgcttc
      421 ttcagccaga ataactttca tctggttctg tgcgcccggg cggatatatt gcagaagcgc
      481 ctgatgtgag gcatcgacgg tttctgatga aacattcaga cggagggcaa taaatgacag
      541 cgccatctgc tgtaaataag aggcatcgac actgttctgc gacacggcaa agggggcatt
      601 aaatgccatt ggcgtcacgg cagtgcgttg ctcattctga agacggtagt tattcccccc
      661 ctgaatgacg ttaacagaga gactgagaac aataagcact gacataaata taaaggcgat
      721 ggccattaca cgactggtac ttaaacgggc accgtgttcc atataacaat cctgatatca
      781 gttctattta atccactgcc ggaaacacga atcgggaaca ttatgaaaaa taccgcgcag
      841 cagggctgtt ggcatatacc agtaaatcag gtcacgtaac caggaactgc cccgcccttt
      901 tttcagtttt ttaatcccga aataaaccag aaccgctgca ccaataccga acagatattt
      961 cgatgttgtg ataccccagc caatacagat tgctgcgggg atcagttcat ccagtggcag
     1021 gccaaaccag cggctctggt tggtcagtgt ttccgggaaa cgatatttct taagtttatt
     1081 ctcgtctccc gacatcgttt tatttcctgt cagaggccaa cgacggccat acccacagca
     1141 ataaatacag agagtatggc aaaaccggcc aggaacttga cgtttttggt catcatgtac
     1201 atgactgcac cgaccagcac ttcagccagg ataacccatg taacaatact ggagtcctta
     1261 ccgaaagttg ctttaaccgt ttcctgacct ttagccatca ggtccgtgcc tttaccagca
     1321 gaagccatag ctaactcagg gaacaacatc atcaaaacag caaccgggat cacggcacga
     1381 accaggcgaa gcatattcag acgtgtaaat ttagaaaaaa acgacttctt tttgacgggc
     1441 gcagaagcac cctgaacact caaaatagca ttcataagtt acctctattg tagatggatt
     1501 aataaaaact aaagttcttt aaatgttgac tcgctctctt ctgtcacctc cctgaaaatt
     1561 tcttcattat aaaaatcagg aaaccgcttc agatgatccc tgagacggat ctggacctct
     1621 atcgttttac ttctgccact acgatctttg gcccgaataa gtcggttatt tgtatcttca
     1681 tcgagataca ccgagaccgt tctgcttata ccgcctctcg cgttacgcct cctcactcaa
     1741 ttcacctctg tttcccgact aataaaattc tgattgcaca gaaaccgatg aaaaaaaaca
     1801 cgccaaaatt ggcgtgtctt atttaacatt tacttaacgc tataaaattc acgaattata
     1861 cttaatagcg aatctatttc tcctttatag atacatgaca ctctgaataa atcaaagtgt
     1921 tcaaaaaatt cttttatttt ccgtttaact ggtctaattc gatcctcaga tgctctgtct
     1981 gtaataccca actctctggc aattgttgcg tgaccaacac ccagcatata aaaagagaaa
     2041 acaaccaatg ttcctggaga aagatcattc catatcgtta atttatcaga agtatttgta
     2101 ttacttccac tctgatttaa cgcaaaaaat ggataatcag gaaacggatt aatttgccat
     2161 aaaacaacag attcgtcatt ataaaaagaa atattctcca tccttatctg atagatttcg
     2221 ccatgaaaat taaaacgacg acaaaatgca gagccatgtc caagagcctg gcattccagc
     2281 tcaagtgatg aaagaaaaat ttcaacctgc aggcgtatat aactctctcc ggataaaggt
     2341 ttatcctctc tggaaaatag ctcaataaac gcgccattgg cataaaggat tttccggttt
     2401 ttgttgcgga cacataccgg aacaggagaa ttcctataga tattctgaac gttaagaatg
     2461 tatttattta cagattgact gttaagtggc ctttctttag gatccagcgc acacatagga
     2521 acctccgtta aggattctat ggacagtcga tgcagggagg ggataac
//
LOCUS       DADKXM010000257         2546 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.260, whole genome shotgun sequence.
ACCESSION   DADKXM010000257 DADKXM010000000
VERSION     DADKXM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.260"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            123..302
                     /locus_tag="JQR05_004049"
     CDS             123..302
                     /locus_tag="JQR05_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326009.1"
                     /translation="MTLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDF
                     MMACDMYFRWGHFNK"
     gene            complement(495..582)
                     /locus_tag="JQR05_004050"
     tRNA            complement(495..582)
                     /locus_tag="JQR05_004050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(546..548),aa:Ser,seq:gga)
     gene            816..1754
                     /gene="ghrA"
                     /locus_tag="JQR05_004051"
     CDS             816..1754
                     /gene="ghrA"
                     /locus_tag="JQR05_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY5326010.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGCKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1809..2546
                     /locus_tag="JQR05_004052"
     CDS             1809..2546
                     /locus_tag="JQR05_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY5326011.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHLVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
BASE COUNT          617 a          576 c          647 g          706 t
ORIGIN      
        1 ttcaggcgaa tgctgtttac ggggtttttt actggttgat actgtttttg tcatgtgagt
       61 cacctctgac tgagagttta ctcacttagc cgcgtgtcca ctattgctgg gtaagatcat
      121 ggattacgct ggtgtcggta ttggcaacgc ttgtggaatg gcggatttgg tttgaagcct
      181 attttttact ctgctatgta ccgtgcttaa tttactattt tttccattgt cgattcctct
      241 ggcataacga ttttatgatg gcatgtgaca tgtatttccg ttgggggcat tttaataagt
      301 gaggaagtta caggaagtga ccagataacg catatatgtt ctgtactatc ttgcgcattt
      361 tgattgttga ctgagtaacc agacagttga tgtgcacaat ttcccctcgc cctaacagac
      421 gtgggcgggg gcacccggtg tgtacaatat acgaaaaaaa agcccgtact ttcgtacgcg
      481 ctcttcttta aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca
      541 cactttccag gcgtgctcct tcagccactc ggacacctca ccaaattgtc gttcctgtct
      601 tgctggaacg ggcgctaatt tagggaaatc atgacctgag gtcaacaaac tttttgaaaa
      661 aatcgcgagt ttattcaaac ttcaatcaat gtgtggtttt aataagcgaa atctgctttt
      721 tttgccaccg accacggatt tgttatgctg gtggcctttg tagatcataa cgataagtgc
      781 gaataaattt cgcacaacgc ttttcgggag tcagtatgga tatcatcttt tatcacccaa
      841 cgttcgatac ccaatggtgg attgaggcac tgcgcaaagc tattcctcag gcaagagtca
      901 gggcatggaa aagcggagat aatgactctg ctgattatgc tttagtctgg catcctcctg
      961 ttgaaatgct ggcagggcgc gatcttaaag cggtgttcgc actcggggcc ggtgttgatt
     1021 ctattttgag caagctacag gcacaccctg aaatgctgaa cccttctgtt ccactttttc
     1081 gcctggaaga taccggtatg ggcgagcaaa tgcaggaata tgctgtcagt caggtgctgc
     1141 attggtttcg acgttttgac gattatcgca tccagcaaaa tagttcgcat tggcaaccgc
     1201 tgcctgaata tcatcgggaa gattttacca tcggcatttt gggcgcaggc gtactgggct
     1261 gtaaagttgc tcagagtctg caaacctggc gctttccgct gcgttgctgg agtcgaaccc
     1321 gtaaatcgtg gcctggcgtg caaagctttg ccggacggga agaactgtct gcatttctga
     1381 gtcaatgtcg ggtattgatt aatttgttac cgaatacccc tgaaaccgtc ggcattatta
     1441 atcaacaatt actcgaaaaa ttacctgatg gcgcgtatct cctcaacctg gcgcgtggtg
     1501 ttcatgttgt ggaagatgac ctgctcgcgg cgctggatag cgggaaagtt aaaggcgcaa
     1561 tgctggatgt ttttaatcgt gaacccttac cgcctgaaag tccgctctgg caacatccac
     1621 gcgtgacgat aacaccacat gtcgccgcga ttacccgtcc cgctgaagct gtggagtaca
     1681 tttctcgcac tattgcccag ctcgaaaaag gggagagggt ctgcgggcaa gtcgaccgcg
     1741 cacgcggcta ctaataaagc atcaggattc ctgctatcct tggcgggaat tgaatacagg
     1801 agagagttat gtatcccgtc gaccttcata tgcataccgt tgccagcaca catgcatata
     1861 gcacattgag tgattacatt gcccaggcca aacaaaaggg cattaaactt tttgcgatca
     1921 ccgatcatgg cccggatatg gaagatgcgc cgcatcactg gcacttcatt aacatgcgta
     1981 tctggccgcg agtggttgat ggggtaggga tcctgcgcgg cattgaagct aacattaaaa
     2041 atgttgatgg tgaaattgac tgcagcggta aaatgtttga ctcgctggat ctgattattg
     2101 ccggttttca tgagccggtt tttgcgccac atgacaaagc gaccaataca caagcgatga
     2161 tcgccactat cgccagcggc aatgtgcata taataagcca tcccggaaat ccgaaatatg
     2221 aaatagatgt taaagccgtt gctgaagcag ccgcgaaaca tctggtggcg ctggaaatca
     2281 ataattcctc atttttacac tcacgtaagg gcagtgaaga caactgtcgt gcggtagctg
     2341 cagcggtacg tgatgctgga ggttgggtgg cattaggctc ggattctcac accgcgttta
     2401 ccatggggga atttgaagag tgtcttaaaa tcctcgacgc ggtagatttt ccgccagagc
     2461 gcattttgaa tgtttctccg cgccgcttac tgaacttcct tgaatctcgc ggtatggcac
     2521 cgattgcgga atttgcagac ctttaa
//
LOCUS       DADKXM010000258         2501 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.261, whole genome shotgun sequence.
ACCESSION   DADKXM010000258 DADKXM010000000
VERSION     DADKXM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.261"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(105..278)
                     /locus_tag="JQR05_004053"
     CDS             complement(105..278)
                     /locus_tag="JQR05_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5326012.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            346..961
                     /gene="tnpA"
                     /locus_tag="JQR05_004054"
                     /pseudo
     CDS             346..961
                     /gene="tnpA"
                     /locus_tag="JQR05_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1289..2371
                     /locus_tag="JQR05_004055"
     CDS             1289..2371
                     /locus_tag="JQR05_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY5326013.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFVDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          734 a          543 c          516 g          708 t
ORIGIN      
        1 aagtttttgc aggcctgctc agggtctttc agtgactgtt gcaatggctg tgacggagca
       61 tctttaatcc attgcgtttc agtggcattt ttccactcaa ccaatcagga agccattttc
      121 gcgtaaggga tgtctttgtt accggcctta tgattctcat tctgacgtgc cagagtacga
      181 ctgaaaacga aacgacatgc gcctgcgaag cgcctcattt cacgcgcctg ttgaccaccg
      241 ggtcttaacc ggaatttgaa tacctgtagt cgtttcacag ggcaaattat actttggtct
      301 gtgaaaaaaa ctgatatccg acgcggaagg cactgtgttt tcctgatgca tgctcaccgg
      361 gtctttgtta ccaagtaccg acgccagatt tttgaccatg acgcgacaga aaaactacgt
      421 acttactttt caaatgtatg tgctgatttt gaagctgaac tggttgaaat ggatggcgaa
      481 ccagagcacg tccatctgtt aattaactat ccccccaaac tggcgatatc cagtctggta
      541 aacaaacagc ctcaaaggcg tatcgggtag gttactgcga cgggatcgac cagatattgc
      601 tgccaggtat taccgcaaag atgttttgtg gcgtcccggc tattttgcca gtagctgcgg
      661 aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt
      721 gaaaaaacga gccttatatc cccgccctga aggacgggct tttacggtgc actggataag
      781 cctggagatt atctacacct gcatggacat cattgtacta tgccccgtac atgccccatc
      841 acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca gtttgtctca
      901 tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc gtattaaata
      961 aaaccacctc aattacatta ataaatctaa ataaatatca tgcaaataat ttccatggtt
     1021 atttttaggc tatttaaatt aaaaaaataa attatgagtc aatcataaat aacaaccttt
     1081 aatataattt gactaccaaa tttatattcg ttcacaaaat agtcatgcat tgtgtgcaat
     1141 agaaacagtt cagataaaaa tagggatcta cttcacaatt taaatgagaa actaaaactt
     1201 acatcttgaa ataatctcat tgattggatg aatatttgta gtgcagtgac atcattttta
     1261 aataatagtt caaataaaag ggctcacgat gaaaaaatta acagtggcaa tttctgctgt
     1321 agctgcatca gtactgatgg cgatgtctgc tcaggcagca gagatttaca ataaagacag
     1381 taacaagctg gatctgtacg gaaaagttaa tgctaagcac tacttctctt ctaacgatgc
     1441 agatgatggc gacacaactt atgttcgtct gggcttcaaa ggcgaaactc aaatcaacga
     1501 tcaactgact ggtttcggtc agtgggaata tgaattcaaa ggcaaccgtg ctgaatctca
     1561 aggttcttcc aaagataaaa cccgtcttgc cttcgctggc ctgaaattcg ttgactacgg
     1621 ctccatcgat tatggccgta actacggtgt agcatacgat atcggtgcat ggaccgacgt
     1681 tctgccagaa ttcggtggcg atacctggac ccaaacagat gtgttcatga ctggtcgcac
     1741 cacaggtgtt gcaacctatc gtaacaacga cttctttggt ctggttgatg gtctgaactt
     1801 tgctgctcag tatcagggta aaaatgaccg cactgacgta actgaagcta acggtgatgg
     1861 tttcggtttc tccactactt atgagtatga aggattcggt gtaggtgcaa cctatgctaa
     1921 atctgaccgc actaatgatc aggttatcta cggtaacaac agcctgaatg catctggtca
     1981 aaatgctgaa gtatgggcag ctggtctgaa atatgatgcg aacaacatct atctggctac
     2041 cacctattct gaaacccaga acatgactgt ttttggtaat aaccatattg ccaacaaagc
     2101 acaaaacttc gaagtagttg cacaatatca gttcgacttc ggtctgcgtc cgtccgttgc
     2161 ttacctgcaa tctaaaggaa aagacttggg tgcgtggggt gatcaggacc tgattgaata
     2221 tattgatgta ggtgcaacct attacttcaa caaaaatatg tccacttttg ttgattacaa
     2281 aatcaacctg attgataaga gcgatttcac gaaagcatct ggcgttgcta ccgatgatat
     2341 cgttgctgta ggattggttt accagttcta atctgattac tcaaagatat gttgtgggag
     2401 gctttgcctc cccaacatat aagtggcgcc ctcaagccac tttccttaga agcactatct
     2461 tgcttctgac tatataaacc ttctgttata tattaccctt t
//
LOCUS       DADKXM010000259         2439 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.262, whole genome shotgun sequence.
ACCESSION   DADKXM010000259 DADKXM010000000
VERSION     DADKXM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.262"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..565
                     /locus_tag="JQR05_004056"
     CDS             <1..565
                     /locus_tag="JQR05_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5326014.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JQR05_004057"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JQR05_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY5326015.1"
                     /translation="MEHSRIKKRNVALIEKCIMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JQR05_004058"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JQR05_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY5326016.1"
                     /translation="MTYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JQR05_004059"
     CDS             2167..2403
                     /locus_tag="JQR05_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY5326017.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          629 a          535 c          624 g          651 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcatcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccggtttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga aatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggtcataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADKXM010000260         2432 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.263, whole genome shotgun sequence.
ACCESSION   DADKXM010000260 DADKXM010000000
VERSION     DADKXM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.263"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..310
                     /locus_tag="JQR05_004060"
                     /pseudo
     CDS             1..310
                     /locus_tag="JQR05_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            336..644
                     /locus_tag="JQR05_004061"
     CDS             336..644
                     /locus_tag="JQR05_004061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326018.1"
                     /translation="MNVEKEIYTGLYLFGNMKLNTSCGGVSAVIKVPTYNSNVLFFIN
                     DSHVSEEDSMENAKCYKFNLEELESIQKAIERALNDIRNLESLSRRNLLKDKAYRNPI
                     "
     gene            815..895
                     /locus_tag="JQR05_004062"
     CDS             815..895
                     /locus_tag="JQR05_004062"
                     /inference="COORDINATES: protein motif:HMM:NF015490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326019.1"
                     /translation="MWYYYTDLTGTVQEVTASDGTLVWAG"
     gene            883..1779
                     /locus_tag="JQR05_004063"
     CDS             883..1779
                     /locus_tag="JQR05_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY5326020.1"
                     /translation="MGWLTSRTGQMYDHDHYYYDKAGNLLTDEYQSAVMDNRLPGYGR
                     DRYRYNEWGELTERRDQQLEWNAQGQLTRVISSNSDTRYQYDALGRCISKATYGRHTD
                     RGERSRTTFVWEGFRLLQETTWQGKRTYLYDAEQPYTPVAAITGRGESQKIWYYHTDL
                     TGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSGAYFEQPQRLPGQYFDEETGLHYNL
                     FRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLGWIDPLGLNSLGIDSNNIFRGDGNY
                     KGGGIGQPKSGTISAQDIIDHL"
     gene            1879..2082
                     /locus_tag="JQR05_004064"
     CDS             1879..2082
                     /locus_tag="JQR05_004064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326021.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQIPGNKVSRCN"
     gene            2100..2420
                     /locus_tag="JQR05_004065"
     CDS             2100..2420
                     /locus_tag="JQR05_004065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326022.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
BASE COUNT          765 a          437 c          657 g          573 t
ORIGIN      
        1 ttatggttta ctcaaaacac aatatcagac gtgtttacac aaggaaaatg ggccgggaga
       61 tcggttgaag aagcgattca tgaggctagt gtcacggggc ggcttcctga tgggctgagt
      121 attagttatg aaaagataat tctgccaagc ggggaggagg tttgggcgac tctaaataat
      181 cgtacgcttt atgtggccca acaggcaaat gttacaatta acccagtaga taaaaatgga
      241 aaaggattaa atcagttgaa taaacttttg gatggaaggg cacccactcc agaacaaccg
      301 gatgttttaa taaaacactg ctaacaggat gcgttatgaa tgtagaaaaa gaaatttata
      361 cagggcttta tttgtttgga aatatgaaat tgaacacatc atgcggaggt gtatctgcag
      421 ttatcaaagt accgacatat aatagtaatg ttttattttt tataaatgat tcacatgtaa
      481 gtgaagaaga tagcatggaa aatgcgaaat gttataaatt taacttagaa gaattagaaa
      541 gtatacaaaa ggcgattgaa agagctttaa atgacatacg aaatctagag tctttatcac
      601 gcaggaattt gcttaaggat aaagcttata gaaaccccat ataatccagc actctgaagt
      661 aatgcgggcg cacaacttgc cctcatatag acgcagggaa tacaagctgt tgctggaaat
      721 accatagcag agcgagcgca ggatgtatct ttacgactcg gagcaggcgt acaccccggt
      781 ggcgagcgtg acggggcgct gagaaagccg aaaaatctgg tattactaca cagacctgac
      841 aggcacggtg caggaggtga ccgcgtcgga cgggacgctg gtgtgggctg gctgaccagc
      901 cgcacgggtc agatgtacga ccatgaccat tattattacg acaaagcggg caacctgctg
      961 acggacgagt atcagagcgc ggtgatggat aaccgcctgc cgggctacgg gcgtgaccgt
     1021 taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcaact ggagtggaac
     1081 gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg atacgcggta tcagtatgat
     1141 gcgctgggca ggtgcatcag caaggcgaca tatggccgcc atacggaccg gggagaacga
     1201 agccggacga cgtttgtgtg ggaagggttc cggctgttgc aggagacaac gtggcaggga
     1261 aagcggacat acctgtacga cgcggaacag ccgtacacgc cggtggccgc catcacgggg
     1321 cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac ggtacacgag
     1381 gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac
     1441 cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct gccggggcag
     1501 tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc gccggagtgc
     1561 gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt atatgcttat
     1621 gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg aatagattca
     1681 aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca gccaaagagt
     1741 ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga tggtcgttta
     1801 acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga ttttttctca
     1861 aaaaaccatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa aaataatcag
     1921 atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa
     1981 attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga ggtgttagtt
     2041 catgggcaaa ttcctggaaa taaagttagc cgatgtaatt agttagtgga gaggaagttg
     2101 tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc catgtgctaa
     2161 aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat tgtttagatt
     2221 gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg agtccagaag
     2281 agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga gctaatttga
     2341 atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat aaaaacggca
     2401 atgaaataga gtctgactaa attttattga ta
//
LOCUS       DADKXM010000261         2316 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.264, whole genome shotgun sequence.
ACCESSION   DADKXM010000261 DADKXM010000000
VERSION     DADKXM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.264"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..520
                     /locus_tag="JQR05_004066"
     CDS             <1..520
                     /locus_tag="JQR05_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5326023.1"
                     /translation="WVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENKE
                     SLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRSD
                     HSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDY
                     GGSSGTETRPLQ"
     gene            549..>2316
                     /locus_tag="JQR05_004067"
     CDS             549..>2316
                     /locus_tag="JQR05_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5326024.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVLGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          692 a          544 c          504 g          576 t
ORIGIN      
        1 atgggtagat aatatagatg gtaaaagccg tgccccattt attataaccc caccgctatt
       61 tcgtcttgag gctggcgatg actcatcact gcgaattatt aaaacagctg ataacctgcc
      121 tgaaaataaa gagtcgctgt tctacattaa tgttcgtgcc attccagcaa agaaaaaatc
      181 agatgatgtt aatgctaacg agttgacgct ggtatttaaa acacggatca aaatgtttta
      241 tcgccccgca cacctgaagg gacgggtaaa cgatgcgtgg aaatcactgg aatttaaacg
      301 tagtgaccat tcactcaata tatataaccc aactgaatat tacgtcgtat ttgccggact
      361 ggcagtcgat aaaaccgatc tcacaagcaa aattgaatat atcgcgcccg gagaacataa
      421 acagttacca cttccagcat ctggcggaaa gaacgtgaaa tgggctgcga tcaacgatta
      481 tggcggcagt tccgggacag aaactcgtcc actgcaataa aaaatataaa aacacaggtc
      541 atcagggaat gcctcaacga caccaccagg gacataaacg cacaccgaaa cagttggcgc
      601 tcattatcaa acgttgtttg ccgatggtgc tcactggcag cggcatgctt tgcactaccg
      661 ctaacgccga agagtattat ttcgacccca ttatgctgga aactacaaaa agtggtatgc
      721 aaacaaccga tctgtcacgt ttttcaaaaa aatacgcaca actaccagga acttatcagg
      781 ttgatatctg gctgaataaa aagaaggttt cacagaaaaa aattacattt accgccaatg
      841 cagagcaact tctgcagcca cagtttacgg tagaacaact acgtgagctg ggtattaagg
      901 tggatgaaat cccggcgctg gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac
      961 aaatcattcc cggtacagct gctgaatttg atttcaatca tcagcgactt aatttgagca
     1021 ttccccaaat tgcactgtac cgtgatgcaa gaggttacgt ctccccttct cgttgggacg
     1081 atggtatacc aacgctgttt accaactact cgtttacagg ttctgataac cgttaccgcc
     1141 agggcaatcg tagccaacga cagtacctga atatgcaaaa tggtgctaat tttggcccct
     1201 ggcgattacg taactattct acgtggacac gcaacgatca ggcgtcaagc tggaacacta
     1261 tcagtagtta tttacaacgt gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa
     1321 gcgccaccag cggcagtatt ttttccagct acacctttac tggcgtgcaa ctcgcttccg
     1381 acgataatat gttgccaaac agccagcgcg gatttgcccc aacggtactc ggtatcgcaa
     1441 acagtagtgc aatcgtgact atcaggcaaa atggttatgt gatctatcaa agcaacgtgc
     1501 cagcgggtgc ctttgaaatt aacgatctct acccctcttc caacagcggc gatttagaag
     1561 tcacgattga agaaagtgac ggtacacaac gtcgctttat ccagccttat tcttcattac
     1621 ccatgatgca gcgacctggg catctaaaat atagcgcgac cgctggacgc tatcgcgctg
     1681 atgcaaacag tgatagcaag gaacccgaat ttgctgaagc cacggcaata tatggtttga
     1741 ataatacttt tacgctgtat ggcggcctgc tcggttctga agattattat gcgctgggga
     1801 tcggtatcgg cggcacactt ggcgcactgg gcgcgttgtc gatggatatc aacagagctg
     1861 acacccaatt cgataaccag cactcttttc atggctatca atggcgtacg cagtacatca
     1921 aagatatccc ggaaaccaac accaatatcg ctgttagcta ctatcgctat accaacgatg
     1981 gctattttag ttttgatgaa gccaataccc gcaattgggg ctataacagt cgccaaaaaa
     2041 gtgaaattca attcaacatc agccagacaa tatttgatgg ggtaagtctg tatgcctccg
     2101 gttcacagca agactattgg ggcaataacg agaaaaacag taatatctct gttggggttt
     2161 ccggccagca atggggaatt ggttacagcc tgaattatca atacagccgc tacactgatg
     2221 aaaataatga ccgtgcactc tctttgaatc tcagtattcc gttagaacgc tggtggattt
     2281 gcccctatat ttccagacat ctgttatcac ttaacc
//
LOCUS       DADKXM010000262         2315 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.265, whole genome shotgun sequence.
ACCESSION   DADKXM010000262 DADKXM010000000
VERSION     DADKXM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.265"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            27..980
                     /locus_tag="JQR05_004068"
                     /pseudo
     CDS             27..980
                     /locus_tag="JQR05_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1021..2016)
                     /gene="pgl"
                     /locus_tag="JQR05_004069"
     CDS             complement(1021..2016)
                     /gene="pgl"
                     /locus_tag="JQR05_004069"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY5326025.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          625 a          582 c          534 g          574 t
ORIGIN      
        1 cgtcgcttcc attttccgga gtaataatga agcatgaatt atcaagtatg aaggcatttg
       61 tcatactggc agagtccagt tcatttaaca atgccgctaa attactcaat attacgcaac
      121 ctgcattaac gcgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc
      181 gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctcccg gaggcgcggg
      241 aattaataaa gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc
      301 gtggtatggt gacattagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa
      361 tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga
      421 ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg
      481 tcacaaattc atccattgat tttaccccac tggtcaacga gccattcgtg ctggcctgtc
      541 ggcgtgacca tccattggcc aaaaagtagc ttgtagaatg gcaggagtta gtgggttaca
      601 aaatgattgg cgtacgttcc tccagcggca accgattgtt gatagaacaa cagctagccg
      661 acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac
      721 tagtggaggc aggactgggg atttcggcgc ttcctggtct ggcaatgccc catgcgccct
      781 attcttcgat tattggtatc ccactggtgg aacccgttat acgtcggaca ttggggatta
      841 ttcgccgcaa agatgccgtg ctttctccgg cagctgaacg cttttttgcc ttgctgatta
      901 atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg
      961 cgttacagga gataagttaa tacaccggcg caggagagac tgcgccggtt aaatcagcgg
     1021 ttagtgtgcg ttaaccacca cccacattgg tccctgcccg accgcatagc ggcctttttc
     1081 atgcagtagc ccctgctcgc caacaatttc gtataccgag atgtggtgag atttttgccc
     1141 agcggcaatc agatacttgc cgctgtaatc aacattgaag ccgcgcggct gggtttccgt
     1201 tggctggaag ccttctttac tcaacacgct gccatcttcc gaaacgctga aaacggtaat
     1261 cagactggcg gtacggtcgc aggcgtataa atggcgacca tccggggtga tatgaatatc
     1321 agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc
     1381 gatattgccg tgcggatctt tcagttccca gacatccact gagctgttta actcattgac
     1441 gcaatacgca tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac
     1501 ggtagtcact tccgcagggt cctgcgccac cagatgacca tcatcgctga ccgtaaacag
     1561 gcaaatgcga tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt
     1621 ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgcccactg gcaggccatc
     1681 ttccagacgc gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc
     1741 ctggtgatcg gtggaaatat gcgtcggact acccggcagc acagactctg cggcaaaggt
     1801 cagtgcgcca tcgtccgggg caatacgata cgccaggacg cgaaactcag ggcgaacacc
     1861 aacatagaga taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc
     1921 gacaacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg
     1981 gctctcaggg ctggcgatat aaactgtttg cttcatgaac acacctcaaa aatgataaat
     2041 cccttatctt tcatacacat aacaacactt actggataaa aaacagacag caaaacacga
     2101 taaaatacaa tatcgcacat caattaaatc cataagttag cactccattt cgaatggcat
     2161 attactaaaa tattagtcac cgacacgaat ttgacaatgc cacagatatt ttcgcgacta
     2221 ttcagggagt atgactctcc ccgcttttgt ttacctgtat caacgaccgt aaacactatg
     2281 ccataacaac ttctggccaa cttcccattc gctca
//
LOCUS       DADKXM010000263         2302 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.266, whole genome shotgun sequence.
ACCESSION   DADKXM010000263 DADKXM010000000
VERSION     DADKXM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.266"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            157..513
                     /locus_tag="JQR05_004070"
     CDS             157..513
                     /locus_tag="JQR05_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326026.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTADQTRTVTG
                     VLRITDTNNDIFTVLL"
     gene            510..1751
                     /locus_tag="JQR05_004071"
     CDS             510..1751
                     /locus_tag="JQR05_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326027.1"
                     /translation="MSENKSFSTAVPAVQISDSGLNVPDEADILSGRLADFSAALGGA
                     MSTSLSSPQGQLASSESAIIADKNDQLLYIVNQINPDFSSGRFQDAIGRIYFLERRGA
                     TGTTVTATCTGLVGTLIPAGSMAQDEAGYKYVSQSDVTIGASGQVDAVFLNLSTGPVG
                     CPVGTLNKIYKAIPGWSGVTNASAGVPGSDEETRADFENRRRNSVARNARNILEAIRG
                     EILSTVENVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAP
                     PGIDMNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQ
                     QSVLDAFNGTDGGLRARGASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVT
                     FGIDEIPVLDVSDISVKLQEA"
     gene            1753..2302
                     /locus_tag="JQR05_004072"
                     /pseudo
     CDS             1753..2302
                     /locus_tag="JQR05_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
BASE COUNT          536 a          568 c          647 g          551 t
ORIGIN      
        1 ggcgataccg ccgtcagtaa ggggcttgac gttaccgggc agtccaggct ttccggcggt
       61 tcgaacatcg ggggtattga ttttggaaac cacgttcacg gcggcgttga gtccggcggc
      121 tcaaccacgc agggaccccg gtaaacagga gaaaacatgc agtcacgatc acttcttctt
      181 gacaccggga catgggatat cctgcttgat gataccggaa atcttgccat tactgataat
      241 ccccatgcgg tagctcagga tgtggcgtgt gcgtgcagta cttttctggg ggagtgctgg
      301 tacgactcaa cggctggcat accttactgg ccacgcatcc tcggacactg gcccggcaca
      361 caactggtga atgccactct gcaacaggaa gcacttaaac tgccgaccgt gagcgccgct
      421 gtctgccagg tcacagctga tcaaacccgg acagtaacag gtgtgctgcg tattacagat
      481 accaataatg acatttttac ggtactgcta tgagtgaaaa taaatctttt tctaccgcag
      541 tgcccgctgt acagatttcg gatagcgggc tgaatgtgcc ggatgaagcg gatattctga
      601 gcggcaggct tgctgatttt tcggcggcgc tgggcggggc catgagtacc agtctgagca
      661 gtccgcaggg acaacttgca tccagcgaaa gcgccattat tgcggataaa aacgatcagt
      721 tgctgtatat cgttaaccag atcaaccctg acttttccag tggtcgcttt caggatgcaa
      781 taggcaggat ttatttcctg gagcgccgcg gggccacagg tacgacagta acagcgacct
      841 gtaccgggct ggttggcacg ctgatcccgg cgggcagtat ggcgcaggat gaggccggtt
      901 ataagtatgt cagtcagtca gacgtcacta tcggcgcatc agggcaggtt gatgcggtat
      961 ttctgaattt gtccaccggc cccgtcggct gtcctgtggg gacgctgaat aaaatttata
     1021 aggcaatacc tggctggtca ggtgtcacta acgccagtgc cggtgtgccg ggcagcgatg
     1081 aggaaacccg tgcggacttt gaaaaccgcc gacgtaattc agttgcccgt aatgcccgga
     1141 atattctgga agccatccgg ggtgaaatac tctccacggt ggaaaacgtg gtggatgttt
     1201 acgtcaccca taatccgaaa aaaacagaac aaaaagccgg agtcagtcag tatccattaa
     1261 cacccggttc gctttatgtt ggcgtgtacg gcggcagccc ggcagatatc gcggcggcca
     1321 tctggcgtaa ggctccgccg ggtattgata tgaatggcaa cacaacgttc actgttgcag
     1381 ataaggagta cgatccgccg tatcctgaat acgtgatcag ctggcagaca ctcaaacccg
     1441 tcagtctgca tgtcagtgtg acgctgaaaa aaagtgacta cctgccttca gatattaccc
     1501 gacaggtaca gcaatccgtg ctggacgcat ttaatggtac agatggtggt ctgcgggcaa
     1561 ggggtgcctc tgttgtctct gccgggcgct actatgccgg catttacaaa accgatccgg
     1621 aacatattga tattctgggc cttactgtga gccgtgacgg ttcgtcatgg acaacggctg
     1681 tcactttcgg gatagatgag attccggttc tggatgtgtc ggatatcagt gtgaaactcc
     1741 aggaggcata acgtgcagaa tgtggctgcc actgtgcttg cgcagtatgc tgccagcccc
     1801 cgactcaatg ccctcattaa cagctttaac gcagcgcttt cccccgacag ttttatcagt
     1861 gatttttatg gtcttatctg gaacatcgat accgcagaaa agtatggtct tgatgtctgg
     1921 ggaaagattg tgggtgtcag tcgccggctg acggtaaagg acgattttaa ttacctggct
     1981 tcagcgagtc caggatggac accccggtaa tggatgatcc ctgtccgttt aatcaggcac
     2041 cgttttacaa cggaaaatcg gatacccgga ctgttgacct gtctgatgct gtataccggc
     2101 ggctgatact gatgaaagcc atgtcgaaca ttactgactg ttccgttccg gatattaacc
     2161 ggatgctgag atttatgttc ggaaaaaaac ggcgggctta tgttctgaat aatggtgggc
     2221 tgaggatgag ttacatcttt gagtccgcgc tctcgttggc agaactggcg attatccagt
     2281 cgtcgggtgc actgccatcc cc
//
LOCUS       DADKXM010000264         2246 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.267, whole genome shotgun sequence.
ACCESSION   DADKXM010000264 DADKXM010000000
VERSION     DADKXM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.267"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            33..728
                     /gene="aqpZ"
                     /locus_tag="JQR05_004073"
     CDS             33..728
                     /gene="aqpZ"
                     /locus_tag="JQR05_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY5326028.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLINIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1049..2242
                     /locus_tag="JQR05_004074"
     CDS             1049..2242
                     /locus_tag="JQR05_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY5326029.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATVALINGASLTLTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          552 a          490 c          587 g          617 t
ORIGIN      
        1 tttcacaggg tcaattttta attgaggtgg atatgttcag aaaattagca gctgaatgtt
       61 ttggtacttt ctggcttgtt tttggtggct gtggtagtgc tgtactggcc gcaggcttcc
      121 cggaattagg cataggtttt gccggcgtgg cgctggcatt tggtttgacc gttctgacga
      181 tggccttcgc tgttggtcat atttctggtg gtcattttaa cccggcggtc actattggtt
      241 tatgggctgg cggacgtttt ccggcaaaag aagtcgttgg ctacgtaatt gcccaggttg
      301 tcggcggtat tgttgcagcg gcgctgctgt atttaattgc cagtggtaaa acgggttttg
      361 acgcggcagc cagcggtttt gcttctaacg gttatggcga gcattcacca ggcggttatt
      421 ccatgctttc cgcgctggta gttgaactgg tattgagtgc gggcttcctg ttagtgatcc
      481 acggtgcaac cgacaaattc gctccggcag gttttgcgcc gatcgctatt ggtctggcct
      541 taaccctgat tcacttaatc aatattccgg tgactaacac ttctgttaac ccggcgcgca
      601 gcaccgcggt tgctatcttc cagggcggct gggcattaga acaactgtgg ttcttctggg
      661 tggtgccaat tgtcggcggc attatcggtg gtctgattta ccggactctg ctggaaaagc
      721 gtgattaatt agcattggca ttttatctgc cctggttgtt ttatcttcga cattacaaac
      781 cgcacgggta ttggtggcgt caaaatcgct gaagcgttcc ccgaaagccg gggcagccca
      841 catggatgtg ggctgagggc gcgtcttaca ggacgttacc tcgcgcccga cccggttgcc
      901 gaaggggata agctgaaggt accgcgcagc ggcgattttg ttcgccagag cccgggggtg
      961 caggggctga ggaatcccca gaaaacgaga cagacatagc attatgtgat ctactgattt
     1021 gtgccaacaa ttcaatgaga tcactgctat gcctgttcat aaagtatgtc agaatttctt
     1081 ccgggatgcc ttagcaccat ttcatcagta ccgacaaaat gccctgatgg atgccacagt
     1141 ggcgttaatc aacggagcat ctctgacgct gaccagtatc ggacgttttt tgccgggtaa
     1201 tgcacaagtt aagaataaga tcaaacgtat tgatcgcctc atgggtaatg aggcgctcca
     1261 tcgcgatatt ccaatgattt tcaggaatat cacttcaatg ctgacacggc agttatcttt
     1321 atgtgtaata gccgtagact ggagtggcta tccctcccag gagcatcatg ttcttcgtgc
     1381 cagccttctg tgtgacgggc gttccattcc gttattgagt aaggttgttc cttctgaaaa
     1441 acaaaataat ccattgatac aacatgattt ccttgattcc cttgctcaat ccctgccccc
     1501 cgatgccagg gttattattg tcacggatgc tggatttcag agcgcatggt ttcaccatat
     1561 tacttctctg ggctgggatt ttattggtcg gatcagaaat aacgttcagt attgcctcga
     1621 taacgcgcct gaaagatggt taaaagtgtc ggatagccct gaatgtaaaa caccggaata
     1681 tatgggggct ggaaggctgg ttaaagaaag gaaaaaaagc atccgggggc atttttacac
     1741 ctataagaaa tcggcaaaag ggcgaaaaaa gaaacgttcg aaaggtcaga gtggtttaaa
     1801 caaaacagac aaagaacaga gcaaatcagc aaaagaagcc tggttgatct tcagcagtac
     1861 aaatgacttc agagcgcgag aaatcatcaa gttatacagt cggcgaatgc agattgagca
     1921 aaactttcgt gatgaaaaga acgggcgctt cggatttggt cttcgggcca gcaaaagtcg
     1981 ttcaacagga agaattctgg ttctgagtct gctggcaacg ttgagcacta tcgtaatgtg
     2041 gttactcggt tatcacgctg aaaataaagg gttacacctg aaatatcagg ctaacagcat
     2101 taaatcccgg cgggttatct catatctgac gttagcgaag aatgttcttc gacactcgcc
     2161 acttatttta agacgaacag tactgagcac agttctaaac catctgtcca gaacctaccg
     2221 aaatatggtg ctggtttatt agcagt
//
LOCUS       DADKXM010000265         2167 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.268, whole genome shotgun sequence.
ACCESSION   DADKXM010000265 DADKXM010000000
VERSION     DADKXM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.268"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            207..1922
                     /locus_tag="JQR05_004075"
     CDS             207..1922
                     /locus_tag="JQR05_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY5326030.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1919..>2167
                     /locus_tag="JQR05_004076"
     CDS             1919..>2167
                     /locus_tag="JQR05_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY5326031.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNIST"
BASE COUNT          460 a          558 c          563 g          586 t
ORIGIN      
        1 tttcgttttt tacatctgaa ctttgcaatt ttccattcat tttaatatcc ctccggcgaa
       61 ttatgctcac aacctgaact taacagaaaa caatccgttc agggatacga cgtttgtatt
      121 ttaagaatct gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg
      181 acataaacat aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt
      241 ttacgctgct ggtggcggtg atcacctggt ggaaggttcg caaaacagat accggatcgc
      301 aacaaggcta ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa
      361 tgttaaccaa cctttccacg gaacaactgg ttggtctttc cgggcaggcc tacaaaagcg
      421 gcatgtcggt gatgggctgg gaagtgactt cagcggtgac gctgatcttc ctcgcgctaa
      481 tctttttacc gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac
      541 gttatgataa aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct
      601 gctttctgcc gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg
      661 gggaatcgct acagatttct cacggtgcgg ctatctggct actggtaatt ttgcttggtc
      721 tggcgggaat tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca
      781 tcaacggtat tgggctggtt atcggcgggt tgatggtgcc ggtatttggc ctgatcgcga
      841 tgggcaaggg cagctttatg cagggcattg agcaaattac caccgttcac gccgagaaat
      901 taaactcagt cggtggcccg accgatccct tgccgattgg cgcggcattt accggtttaa
      961 ttctggtgaa caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt
     1021 caaaaagcct ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg
     1081 acccgctggt actggtgctg ccagggttga ttgcgtttca tctgtatcag gatctaccga
     1141 aagccgacat ggcctacccg acgctggtca ataacgttct gccagtgcca ctggtgggtt
     1201 tcttcggcgc ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg
     1261 ccagtacatt attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc
     1321 agcagctggt caccgtcggg cgcaaatttg gtttctttat cgctatcgtt tcggttctgg
     1381 tagcgccgtg gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa cagctcaacg
     1441 gcatttacaa cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc
     1501 cggcgctggc ggcaaaagtg gcgatgggga ttggcataat cagctacatc accatcaact
     1561 atctggtgaa gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg
     1621 tggtcgtgat gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag
     1681 atgcgtttgc ggtggacatg aaaccgtgga aaaacgtcaa gatcgcgtca attggtatcc
     1741 tgttcgcgat gattggcgtc tatgtcgggc tggctgaatt cggcggctac ggtacgcgct
     1801 ggttagcgat gatcagttat ttcatcgctg ccgtagtgat tgtctacctg atttttgaca
     1861 gctggcggca tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag gatagcctat
     1921 gaaacgcccc aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg
     1981 ctatagcggt aaaccgctga atacgcaaaa tattgatagt ctggcggcgg aaggtattcg
     2041 ctttaattcc gcctacactt gttcaccggt ttgtacgcct gcacgcgccg gactattcac
     2101 cggtatctac gctaaccagt ccggcccgtg gaccaacaac gtcgcgccag gcaaaaacat
     2161 ctccact
//
LOCUS       DADKXM010000266         2163 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.269, whole genome shotgun sequence.
ACCESSION   DADKXM010000266 DADKXM010000000
VERSION     DADKXM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.269"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JQR05_004077"
     CDS             complement(<1..251)
                     /locus_tag="JQR05_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326032.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
     gene            complement(488..700)
                     /locus_tag="JQR05_004078"
     CDS             complement(488..700)
                     /locus_tag="JQR05_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5326033.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1098..1463)
                     /locus_tag="JQR05_004079"
     CDS             complement(1098..1463)
                     /locus_tag="JQR05_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY5326034.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPLGDMVEIVEMAMRKAGN
                     SPVTPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(1463..2128)
                     /locus_tag="JQR05_004080"
     CDS             complement(1463..2128)
                     /locus_tag="JQR05_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326035.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
BASE COUNT          542 a          565 c          453 g          603 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgaaaaac atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt
      421 agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
      481 tggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc tggccggttc
      541 ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg acggttaaac
      601 agatgacgat cagggcgatt aacatcgcct tttgctgctt catagcctgc ttctccttgc
      661 ctttcggcac gtaagaggct aacctacatt tgtgagacat agattgggcc tcagattaat
      721 gttaagcgtc ttgcaggacg cgtaatgtta actggggctt ttctctgtct gccttacagt
      781 ggcatgcccg aggcagacag cctcaagcac ccgcagcaat cttaccgata ccgataagaa
      841 aacgctattt tttattgcca gcaccttcta tccaggctaa tttatctgcg tcagaacggc
      901 gcaatgcgct cgcctgagat acgtttactt gccatggtaa caacaggtaa cggcaattgc
      961 tgtttctgtt ttgtttcctt caaaaacccc ggaccatcaa tccggggttt ttgtttgtta
     1021 accccaacgg caaatcgaat acaccaccag cgccaccgcc atcgcaattc ctaccgtggt
     1081 gaatgcctca ggccaggtca ttgattcacc tcctgcggtg gttccggtag cggcatccag
     1141 tgagttgagc tctctacttc aacgccgtct ttgtccacaa aagccatctt attcccgcca
     1201 cgagtggggc aaaaaacctt atcccaggaa ccggggaata cattcccgag atcatcgaga
     1261 agaatcacat cgcaatattc agcaggaact gcatcactac agcttatcca gccagccgga
     1321 gttaccggag agttgccagc cttacgcatg gccatttcca cgatttcaac catatctcct
     1381 agtggaattt tacaaagttg cccaatacat ttctgctgcc tggcatattc gagaatgtgc
     1441 tccagtttgg ttcgattaat cattatttat ctcccttaag catggcagcg cggcaggcgt
     1501 tccagccatc aacataatca aacgtattac tatcgtttgg cccgatttca tccggcacta
     1561 tcggctctgg ttggatagtg acgttggcaa aggcagcacg caaaccggtt ttaatttctt
     1621 cgatttcatc agagccaagc gatgaatctg acagtgcgtg atggaatgcg taagccatgt
     1681 cgtcgtttac tgcaaccggc actgccggcg ctgcgtaaag cggtgttata tctgcccgaa
     1741 aatcacatgc tttatgcagt cgtacccaac gctcaacttc cgctttgtca aaatatatag
     1801 cagtgaacgt attagattcc tggtcaactt gagtaaacgt caccttccac gctacagcta
     1861 cggctttcca tccatcaggt tgtcgcattc gcggggcagc ataaagctca tgcattccat
     1921 caggcagcga gtgacccaca tactcaccga acccgtcaat acacatgccc ccatcttcaa
     1981 caatgcattc cgctacaggc tctgcttcca gtgatgccag agcgataagc gccagttcac
     2041 gaatttcacc gccgtctata tcgtcaatat catcgcggcc agaaatgtta gccagccatt
     2101 gcagtcgctc tttggtaata gtggtcatgc cgtagcccct tcttgatatt tttcaaacca
     2161 gaa
//
LOCUS       DADKXM010000267         2145 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.270, whole genome shotgun sequence.
ACCESSION   DADKXM010000267 DADKXM010000000
VERSION     DADKXM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.270"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..161
                     /locus_tag="JQR05_004081"
     CDS             <1..161
                     /locus_tag="JQR05_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5326036.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JQR05_004082"
     CDS             655..1233
                     /locus_tag="JQR05_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326037.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JQR05_004083"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JQR05_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2145
                     /locus_tag="JQR05_004084"
                     /pseudo
     CDS             1584..2145
                     /locus_tag="JQR05_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          688 a          392 c          529 g          536 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agaccggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagata atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaattat agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataat
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacaa ctggcagaga tggctgtcgc
     1921 tggcggccaa cagtgacgtc cccatgatga agaatgtggc taaaacgata gggaaaaggt
     1981 tgtacggtat cctgaacgcc atgcgccatg gcgtatcaaa cggaaatgcg gaagcactga
     2041 acagcaagat caggctgctg agaataaaag ccaagggcta ccgcaaccgg gagcgcttta
     2101 agctgggagt gatgttccac tatgggaaac tgaatatggc gttct
//
LOCUS       DADKXM010000268         2108 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.271, whole genome shotgun sequence.
ACCESSION   DADKXM010000268 DADKXM010000000
VERSION     DADKXM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.271"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..533
                     /locus_tag="JQR05_004085"
     CDS             <1..533
                     /locus_tag="JQR05_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY5326038.1"
                     /translation="EKLQQAVSKLHIGDGLEKGVTIGPLIDEKAVAKVEEYIADALEK
                     GARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLAPLFRFKDEADVIAQAN
                     DTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVAPFGGIKASGLGREGSK
                     YGIEDYLEIKYMCIGL"
     gene            547..1827
                     /gene="gabT"
                     /locus_tag="JQR05_004086"
     CDS             547..1827
                     /gene="gabT"
                     /locus_tag="JQR05_004086"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY5326039.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAKNCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
BASE COUNT          481 a          555 c          653 g          419 t
ORIGIN      
        1 ccgaaaaatt acagcaggcg gtgagcaaac tgcacatcgg cgacgggctg gagaaaggcg
       61 tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag tatattgccg
      121 atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcgcacgaa cgcggcggca
      181 acttcttcca gccaaccatt ctggtggacg ttccggccaa cgccaaagtg tcgaaagaag
      241 agacgttcgg ccccctcgcc ccactgtttc gttttaaaga tgaagccgat gtgattgcac
      301 aagccaatga caccgagttt ggtcttgccg cctatttcta cgcccgtgat ttaagccgcg
      361 tcttccgcgt cggcgaggcg ctggagtacg gcatcgtcgg catcaatacc ggcattattt
      421 ccaatgaagt ggccccattc ggcggcatca aagcctcggg tctgggtcgt gaaggttcga
      481 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
      541 atgcgaatga acagcaataa agagttaatg cagcgccgca gtcaggcgat tccccgtggc
      601 gttgggcaaa ttcacccgat tttcgctgac cgcgcgaaaa actgccgggt gtgggacgtt
      661 gaaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac cgggcacctg
      721 catccgaagg tggtggccgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc
      781 caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg
      841 ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg
      901 gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag cggcgcgtat
      961 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     1021 atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct gcacggcata
     1081 agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc cgcgccggaa
     1141 gatatcgccg ccatcatgat tgagccggtt cagggcgaag gcggtttcta cgccgcgtcg
     1201 ccagccttta tgcagcgttt acgcgctctg tgtgacgagc atgggatcat gctgattgcc
     1261 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcagatgggc
     1321 gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc gctggcgggc
     1381 gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg cggcacctat
     1441 gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt cgagcaggaa
     1501 aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt gttggcgata
     1561 gccgaaaaac acccggagat cggcgacgta cgcgggctgg gagcgatgat tgccatcgag
     1621 ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga gatcgtggca
     1681 cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     1741 atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga gattatcggt
     1801 cagtgttttg atgaggcgaa gcagtagcac cgcccccatg caggaggcga cgctgcgcgt
     1861 cttgtccggc ctacgagaat ccaagtcgga taaggcgttt acgccgcatc cgacaatctg
     1921 tacgtgaaca ggaataaatc tatatcggcc ggataaggcg gagccgctct ccggcaaaaa
     1981 gaatcaataa caattaaacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     2041 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacacga gttaggcggc
     2101 gggctgaa
//
LOCUS       DADKXM010000269         2105 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.272, whole genome shotgun sequence.
ACCESSION   DADKXM010000269 DADKXM010000000
VERSION     DADKXM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.272"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..814
                     /locus_tag="JQR05_004087"
                     /pseudo
     CDS             1..814
                     /locus_tag="JQR05_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1253..2090
                     /locus_tag="JQR05_004088"
                     /pseudo
     CDS             1253..2090
                     /locus_tag="JQR05_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          522 a          511 c          633 g          439 t
ORIGIN      
        1 ccagtacacc tcagttctgg gccttgcccg tagcagttat ttttatcacc gggctgcact
       61 gaaagccggt gataaatacg ccacgatacg taagatgctg acagatatct ttaacagtaa
      121 ttaccagtgt tatggctatc gtcacctgca tgcgatgctc aggcatgagg gggggcggct
      181 atcagaaaag gttgtacgca gacttatggt ggaggaacag cttgttgtca gtcgtaaccg
      241 tcgtcgtcgc tacagctcat attgcggaga aatcggaccg gctccggata accttatcgc
      301 cagagatttt aaggcggagc aacctaatca gaaatagctg acagatatca cggagttcca
      361 gttccctgca ggtaaagtct ggctgtcacc agtggtggac tgcttcgatg gaaaagttgt
      421 gagctggtct ctcagtacac gccctgatgc tgaactggtc aacactatgc tggatagcgc
      481 tgtcgaaacg ttaaatgctg gcgaacgacc ggtgatacac agtgacagag gtgggcatta
      541 tcgctggcca ggctggctgg aaagagtgaa tgcagcaggt cttattcgct caatgtcccg
      601 taaaggatgt tcacctgata atgctgcatg cgaaggcttt ttcggcagac tgaaaacgga
      661 aatgtattat gggcgtaaat ggtcgggcat cacgccagaa aagttcatgc agcaagtgga
      721 tgcttacatc agatggtata acgagcggcg tataaaatta tcgctgggtg ccgtcagccc
      781 caaaatgtac cgccaacaat gcgggctgga atgataaagc agtccaggaa atcgtccgca
      841 tccccccttc accatttgga ttgtccgctc cacccaacat gttgtttcct taaggttcct
      901 acaccacaaa ggaaactctc atgctgcgca gtggatggct gacgttgccg acaggcggga
      961 acttcgccag ttcaggcaga caccggaaca gcgcttcacg caggaacagg agcatctgca
     1021 gccgctgctg ggtacggact tcgatatccg tcatgtctcc tgggatggct atatcgaggt
     1081 tggcgggaac cgttacagtg tgccggaaag cctgtgtggt cagctggtat caatacggat
     1141 ctcgctggat gatgaactgc ggatctacag taatgagcaa caggtagcat cgcaccgtct
     1201 ctgttcggct gcatacggct ggcagaccgt gccggagcat cacgctccac tctggcagca
     1261 ggcttcggag cgcatggccg aacggctggg ggagatacag aaacgggtaa tcactgtctg
     1321 tgaccgggaa gcggatatct ggcattacct gtattataaa gtcagccacg gacagcgtgg
     1381 tgcgtgctgc acagaatcgc cggctggaag aggcacccgg caagctcttc gaactgccgg
     1441 aagtcctggc aaccgcagga agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag
     1501 cccgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat cccgacaaca
     1561 gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag gacggtgcct
     1621 gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca
     1681 gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa agtggtggta
     1741 catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg ttatccaggc
     1801 gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa
     1861 tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg ttaagctgga
     1921 aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg atggcatgac
     1981 agcaaacgca caggtcgtcc cggttggggc gtcatgtggg atggctggtc agacttcagg
     2041 atatggttga gggttacctg gtgatgaagt ctcttgatca ggagctctga tcaagagaca
     2101 gcagc
//
LOCUS       DADKXM010000270         2102 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.273, whole genome shotgun sequence.
ACCESSION   DADKXM010000270 DADKXM010000000
VERSION     DADKXM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.273"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>2102
                     /locus_tag="JQR05_004089"
     CDS             <1..>2102
                     /locus_tag="JQR05_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY5326040.1"
                     /translation="YRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGD
                     GLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQF
                     SPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYL
                     VEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMT
                     RGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYR
                     AGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFR
                     LPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQL
                     LYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPVSREGSETVSDASGHVALNLPA
                     ADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAAL
                     ESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLS
                     HAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLL
                     SHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLD
                     IRVKTDKTHYQPGELVNV"
BASE COUNT          498 a          549 c          568 g          487 t
ORIGIN      
        1 tatcggcgct atcagatgga agagtacggc ggcgtggacg ttcgcctgta tcgtattcct
       61 gacccgatgg catttttgcg ccagcagaaa aacctgcatc gcattgtggt gcaaccgcaa
      121 tatctgggcg acgggctgaa caatacgctg acctggctgt gggataactg gtacggcaaa
      181 tctcgccgcg tgatgcagcg tactttctct tctcagtcac ggcagaatgt gactcaggca
      241 ttacccgaat tacatctcgg caatgccatt attaaacctt cccgttatgt acagaacaac
      301 cagttttccc cgctgaaaaa atatcccttg gtggaacagt tccgttatcc actatggcag
      361 gctaaaccgg tcgagccgca gcaaggggta aaactggaag gcgcatccag caatttcatc
      421 tcgccgcagc cgggtaatat ttatattcct ctcggccaac aagagccggg gctgtacctc
      481 gtcgaggcga tggtaggtgg gtatcgggcg acgacggtgg tgtttgtttc cgataccgtg
      541 gcgcttagca aagtgtcagg taacgagctt ctggtatgga ccgcgggtaa aaaacagggt
      601 gaagcgaagc ccggctcaga gatcttgtgg actgacggtc ttggcgtgat gacccgcggg
      661 gtgaccgatg acagcggtac tttgcagtta caacatatat cgccagaacg ttcatacatt
      721 ctgggtaagg atgctgaagg cggcgttttt gtctccgaga acttcttcta cgaaagcgaa
      781 atctacaaca cccgcttgta tatttttacc gatcgcccgc tatatcgcgc aggcgatcgt
      841 gtcgatgtta aagtgatggg ccgcgagttc cgcgatccgt tgcattcatc ccccatcgtc
      901 agcgccccgg cgaagctttc ggtgctggac gctaacggca gtctgttgca aaccgtcgat
      961 gtcacgctgg atgcgcgcaa tggcgggcag ggaagtttcc gcctgccaga aaatgccgtt
     1021 gccggaggtt atgagttacg tcttgcttac cgcaatcagg tctatagcag cagttttcgc
     1081 gtggcaaact acatcaagcc acatttcgag attggtttag ctctcgacaa aaaagagttc
     1141 aaaactggcg aagcggtcag cggcaaactg caactgctct acccggatgg cgagccggta
     1201 aaaaatgccc gcgtgcagtt aagtttgcgc gctcagcaat tatcaatggt cggtaacgat
     1261 ttgcgttatg ccggacgttt ccccgtgtcg cgggaaggca gcgaaacggt gtccgacgcc
     1321 agcggtcatg tggcgttaaa tctccccgcc gccgataaac cgagccgcta tttgttaacc
     1381 gtctccgcca gtgacggcgc ggcgtatcgc gtcaccacca ccaaagagat cctcattgaa
     1441 cgcggcctgg cgcattactc tttaagtacc gccgcacaat acagtaatag cggcgagtcg
     1501 gttgtgttcc gttatgccgc gctggaatct tcaaaacagg ttcctgttac gtatgaatgg
     1561 ttgcgtctcg aagaccgcac gagccatagc ggagagctac cgtcaggcgg caaatccttt
     1621 accgtcaatt tcgctaaacc tggcaactac aatctgacat tacgcgataa agacggctta
     1681 attctcgctg ggttaagtca tgccgtcagc ggtaagggca gcacggcgca tactggtacg
     1741 gtagatatcg tggcggataa aacgctgtac cagccaggcg aaaccgcgaa gatgctgatt
     1801 actttcccgg agccaattga tgaagcgtta ttgacgctgg aacgcgatcg cgtggaacag
     1861 cagtcgctgc tttcgcatcc ggcaaactgg ctaacgctac aacgtttaaa cgatacccag
     1921 tatgaagccc gggttccagt gagcaattcc tttgcgccta acatcacttt ttcggtgctg
     1981 tatacccgta atggtcagta cagttttcag aacgccggga tcaaagttgc cgttcctcag
     2041 cttgatatcc gggtgaaaac ggacaaaacc cattaccagc ctggtgaact ggtcaatgtc
     2101 ga
//
LOCUS       DADKXM010000271         2043 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.274, whole genome shotgun sequence.
ACCESSION   DADKXM010000271 DADKXM010000000
VERSION     DADKXM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.274"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(220..384)
                     /locus_tag="JQR05_004090"
     CDS             complement(220..384)
                     /locus_tag="JQR05_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326041.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="JQR05_004091"
     CDS             1286..1570
                     /locus_tag="JQR05_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5326042.1"
                     /translation="MIYGGFMKSSVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="JQR05_004092"
     CDS             1558..2043
                     /locus_tag="JQR05_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5326043.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          527 a          426 c          583 g          507 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgct gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgtgaga gtgtcacgta cggttccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgttt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caagtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 ggtgaagctg tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tag
//
LOCUS       DADKXM010000272         2016 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.275, whole genome shotgun sequence.
ACCESSION   DADKXM010000272 DADKXM010000000
VERSION     DADKXM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.275"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1414
                     /gene="fimD"
                     /locus_tag="JQR05_004093"
     CDS             <1..1414
                     /gene="fimD"
                     /locus_tag="JQR05_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
                     /protein_id="HAY5326044.1"
                     /translation="GGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHE
                     GDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDCDYDGEHRDE
                     PIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSW
                     VGISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRN
                     SNGQNSWLAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYER
                     DQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAV
                     MPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQ
                     GGKPVPFGSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLP
                     KQSLQQAVTVISAVCTHPGS"
     gene            1427..>2016
                     /locus_tag="JQR05_004094"
     CDS             1427..>2016
                     /locus_tag="JQR05_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY5326045.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPRQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGEMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGLMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVP"
BASE COUNT          515 a          438 c          515 g          548 t
ORIGIN      
        1 cggcgggacg caattatctg ccaattacac cgccttttta ttaggactgg ggcgcaatct
       61 cggaaactgg ggggcagtgt cgctggatgt gacccatgcg cgcagtcagt tagccgacga
      121 cagtcgtcat gagggggatt ccattcgctt cctctatgcg aaatcgatga acaccttcgg
      181 caccaatttt cagttaatgg gttaccgcta ttcgacacaa ggtttttata cccttgatga
      241 tgttgcgtat cgtcgaatgg aggggtacga atatgattgc gattacgacg gtgagcatcg
      301 ggatgaaccg ataatcgtga attaccacaa tttacgcttt agccgtaaag accgtttgca
      361 gttaaatatt tcacaatcac ttaatgactt tggctcgctt tatatctctg gtacccatca
      421 aaaatactgg aatacttcgg attcagatac ctggtatcag gtggggtata ccagcagctg
      481 ggttggcatc agttattcac tctcattttc gtggaatgaa tctgtaggga tccccgataa
      541 cgaacgtatt gtcggactta atgtttcagt gcctttcaat gttctgacca aacgtcgcta
      601 cacccgggaa aatgcgctcg accgcgctta tgcctccttt aacgccaacc gtaacagcaa
      661 cgggcaaaat agctggctgg caggtgtagg tgggacctta ctggaaggcc acaacctgag
      721 ttatcacgta agccagggtg atacctcgaa taatgggtat acgggcagcg ccacggcaaa
      781 ctggcaggcc gcttacggta cgctgggggt cgggtataac tacgagcgcg atcaacatga
      841 cgttaactgg cagctgtctg gcggtgtggt cgggcacgaa aatggcataa cgctgagcca
      901 gcctttaggg gataccaatg ttttgattaa agcgcctggc gcaggcggtg tacgcattga
      961 aaatcaaact ggcattttaa ccgactggcg cggctatgcg gtgatgccgt atgccacggt
     1021 ttatcggtat aaccgtatcg cacttgatac caatacgatg gggaattcca tcgatgttga
     1081 aaaaaatatt agcagcgttg tgccgacgca aggcgcgttg gttcgtgcca attttgatac
     1141 ccgcataggc gtgcgggcgc tcattaccgt tacccagggc ggaaaaccgg tgccgtttgg
     1201 atcactggta cgggaaaaca gtaccggaat aaccagtatg gtgggtgatg acgggcaagt
     1261 ttatttaagt ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa
     1321 ctcacgctgc attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt
     1381 tatttcggca gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg
     1441 cagattatta ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg
     1501 tgcaaatagt actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag
     1561 caccaataac cagccaaggc agattgttgt tttaccggaa aaatccggct gggtaggtgt
     1621 ctcagcaatt tgtccacccg gtacgctggt gaattataca taccgtagtt atgtcaccaa
     1681 ctttattgtt caggaaacta tcgataatta taaatatatg caattaaatg attatctatt
     1741 aggtgagatg agtctggttg atagtgtgat ggatattcag ttccccccgc aaaattatat
     1801 tcggatggga acagatccta acgtttcgca aaaccttcca ttcgggctga tggattctcg
     1861 tttaatattt cgtttaaagg ttattcgtcc ctttattaac atggtggaga tccccagaca
     1921 ggtgatgttt accgtgtatg tgacatcaac gccttacgat ccgttggtta cacctgttta
     1981 taccattagt tttggtggcc gggttgaagt accgca
//
LOCUS       DADKXM010000273         2011 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.276, whole genome shotgun sequence.
ACCESSION   DADKXM010000273 DADKXM010000000
VERSION     DADKXM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.276"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(246..1394)
                     /locus_tag="JQR05_004095"
     CDS             complement(246..1394)
                     /locus_tag="JQR05_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5326046.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            1709..>2011
                     /locus_tag="JQR05_004096"
     CDS             1709..>2011
                     /locus_tag="JQR05_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326047.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVK"
BASE COUNT          623 a          511 c          435 g          442 t
ORIGIN      
        1 ccatcgcaac tgtttttcct gaacattccc agccatagac aaatccttcg ccaaaattaa
       61 aggggcatct gtaaaacgag ggattatgcg cggcaaataa gaagtgcggt aatagcgaaa
      121 tgcaaaatta taacctgctg cacattttat ttttaaacga ataaaatttg gaaaaaagca
      181 agtcagatag aagaaacaaa aaagcagagt gtgaaacagt aagttcacac tttgcttgaa
      241 ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgaag cagcatcagc
      301 aaatagataa acgatacgct ggcgatcatg ataaacaata aattatcgga gaaattctgc
      361 attagcatag cggtaaatga cgggccaagc agacttccca cggtatagct caacagtaag
      421 gcttggttca tcgccaccag ttgatgatgt tcaactttct cacaagccca ggccatcgcc
      481 accggatata gcgtaaagcc agcggcaccg aggatgaata acgctggggc catcgccgcc
      541 tggctaagca tcgcaatact gccgagaatg acgacaaaga cctgaacacg caaaaccagc
      601 agtcgaccaa acttatccgc cagacgtcca atcggccatt gtccgaggat acccgcactg
      661 accagtaccg ccatccagaa accaatactg gcattgctca cccctttgtg attgaggtac
      721 agaggcatca ggccatatag agatcccaga acgattcctg agataatgca gccattcacg
      781 ccaagccgcg cctgacggag ttttagcatt gccgtaattg acgtcgaatc atggttttca
      841 gcctgctgat tcaggacacg cgtaaacaac agcggtaaga tccctgccaa tgtcaaacct
      901 gtaacccacg gcaatacgct catcagctcg gttgagactt tgctgaccag taactggcct
      961 aaaaacgttc ccacgtaata aaccatcata tacgcagcaa gcaaacgccc acggttacgt
     1021 gacgtcccac tgcacatcag cgcgctctca acaaccaccc aaatcatggc acagccgacg
     1081 cccgcgacaa aacgccacgc caaccagctc cagaaaccaa tcatcaggcc aaggccggca
     1141 cagccagcag caaaaatgaa ggaggacaga taatagctgc ggttaaagcc aatgcgttta
     1201 atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga tgagctgaca
     1261 acgcctacct gccatgtgga catgtgttcc tgggcgagcc aaagcggcac gagtgtattt
     1321 aacaccgcga tcgccagagt caacaaaagc aggccagaca gcaaaagcat gacaggccgg
     1381 gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc atcatgccac
     1441 cgccaaaaac attgtcaatg ggtgaaaata cgcgcatatc gcagcggcgg tattatcgat
     1501 ttatattact cattcataac gctaatgctg aagagatgat taatctcatc tgattgtttt
     1561 tattgatcta acacaaattt catacgcttc gttcagtcga aaaatttcat ggtcacagca
     1621 aactcatatt tcccgtctat gctttcagag caatgcatcg cgtctgaccg atttcagacg
     1681 taccactgac tctcgaaagg agaagaggat gaccaaaccg tatgttcgtc ttgataaaaa
     1741 tgatgctgcc gttttgcttg ttgatcacca ggctggttta ctttcccttg tacgggatat
     1801 cgaacccgat aagtttaaaa ataacgtgct ggcgctgggt gacctggcta agtatttcaa
     1861 cttaccaact attcttacca ccagttttga aaccggtcct aacggcccat tagttccaga
     1921 actgaaagca caattccccg atgcacctta cattgctcgc ccgggcaata ttaacgcctg
     1981 ggataacgaa gattttgtaa aagcagtcaa a
//
LOCUS       DADKXM010000274         2007 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.277, whole genome shotgun sequence.
ACCESSION   DADKXM010000274 DADKXM010000000
VERSION     DADKXM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.277"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            42..1238
                     /gene="hemY"
                     /locus_tag="JQR05_004097"
     CDS             42..1238
                     /gene="hemY"
                     /locus_tag="JQR05_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY5326048.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
BASE COUNT          544 a          483 c          502 g          478 t
ORIGIN      
        1 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
       61 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
      121 atgtgctgat ccagaccgac aactacaata tcgaaaccag cgtcacgggc ctggcgatca
      181 tattgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
      241 gcactggcgc gcacacccgt ggatggtttg tcggacgtaa gcgtcgccgt gcgcgtaagc
      301 agaccgaaca ggcgctgctg aaactggcgg aaggtgatta tcagcaagtt gaaaagctga
      361 tggcgaaaaa tgccgatcac gctgaacaac cggtggtgaa ctatctactg gctgccgaag
      421 ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcggagc
      481 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
      541 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
      601 ctgaagtatt acgtctggcg gaacaggcgt atatccgcac aggggcatgg agttctctgc
      661 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
      721 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
      781 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg
      841 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
      901 tcgacggcct gaaacgccag tatgacgatc gcctactgct gccgatcccg cgtctgaaaa
      961 ccaataaccc ggaacagctg gaaaaagtgc tacgccagca aatcaaaaac gtcggtgatc
     1021 gcccgctgtt gtggagcaca ttgggtcagt cactgatgaa gcacggtgaa tggcaggaag
     1081 catcgctcgc cttccgcgcg gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
     1141 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
     1201 gtttgatgtt aacgttgcag aataacccgc cacagtagtt ccttctcacc cggaggaaag
     1261 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcatt
     1321 aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg
     1381 atgaggcata agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt
     1441 ccggagttta tgaggcacta aggcgaacat aagagatgga atgagcatct actcgtttat
     1501 tatgccacag agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa
     1561 tcctctttgc ttcctgagta ataacttcct gagtgaatat ttaacctgag cttgatccta
     1621 cacatactta ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt
     1681 taaaactaaa aacagcatca ataatcaacg cgatataata aacctgcctt acatatcaac
     1741 tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat
     1801 aaataattta tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca
     1861 attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg
     1921 aattttcgtt ttcacccaaa cgtcttgttg ttgctgtcgc cgccgctctt cctctcatgg
     1981 ccagcgcagc agataccccg tcaactg
//
LOCUS       DADKXM010000275         1979 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.278, whole genome shotgun sequence.
ACCESSION   DADKXM010000275 DADKXM010000000
VERSION     DADKXM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.278"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            123..1354
                     /gene="mdfA"
                     /locus_tag="JQR05_004098"
                     /pseudo
     CDS             123..1354
                     /gene="mdfA"
                     /locus_tag="JQR05_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(1395..1679)
                     /locus_tag="JQR05_004099"
     CDS             complement(1395..1679)
                     /locus_tag="JQR05_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326049.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1765..>1979)
                     /gene="ybjI"
                     /locus_tag="JQR05_004100"
     CDS             complement(1765..>1979)
                     /gene="ybjI"
                     /locus_tag="JQR05_004100"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY5326050.1"
                     /translation="LWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKY
                     RAGSNNREGVLDVIDKVLKHEAPFDQ"
BASE COUNT          417 a          473 c          538 g          551 t
ORIGIN      
        1 gcgtagctat actcgcaata atgtaagaat gtgcttaacc gtggtttcag ctacaaaatt
       61 cgctttctcg ttagctgcgc ttttattaaa ctctgcgcga ttattattgg cgaagaaatt
      121 gcatgcaaaa taaattagct tccggtgcca ggcttggacg tcaggcgtta cttttccctc
      181 tctgtctggt gctttacgaa ttttcaacct atatcggcaa cgatatgatc caacccggta
      241 tgttggccgt ggtggaacaa tatcaggcgg gcattgattg ggttcctact tcgatgaccg
      301 cgtatctggc gggcgggatg tttttacaat ggctcctggg gtcgctgtcg gatcgtattg
      361 gtcgccgtcc ggtgatgctg gcgggtgtgg tgtggtttat cgtcacctgt ctggcaatat
      421 tactggcgca aaacattgaa caattcaccc tgttgcgctt cttgcagggc ataatcctct
      481 gtttcattgg cgctgtggga tacgccgcaa ttcaggaatc cttcgaagag gcggtttgta
      541 tcaagatcac cgcgctgatg gcgaacgtgg cgctgattgc tccgctactt ggtccgctgg
      601 tgggcgcggc gtggatccat gtgctgcctt gggaggggat gtttgttttg tttgccgcat
      661 tggcagcgat ctcctttttc ggtctgcaac gagctatgcc tgaaaccgcc acgcgtatag
      721 gcgagaaact gtcactgaaa gaactcggtc gtgactataa gctggtgctg aagaacggcc
      781 gctttgtggc ggggcgctgg cgctgggatt cgttagcctg ccattgctgg cgtggatcgc
      841 ccagtcgccg attatcatca ttaccgggga gcagttgagc agctatgaat atggcttgct
      901 gcaagtgcct attttcgggg cgttaattgc gggtaacttg ctgttagcgc gtctgacctc
      961 gcgccgcacc gtacgttcgc tgattattat gggcggctgg ccgattatga ttggtctgtt
     1021 ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga ccgccgggtt
     1081 aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat tagccctgtt
     1141 tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc aaatgctgat
     1201 ctttaccgtc ggtattgaaa tcagcaaaca tgcctggctg aacgggggca acggactgtt
     1261 taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta tctttttaaa
     1321 agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgattga tttgtgcgat
     1381 caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac catcccttca
     1441 cctacgctgg caagatggcg aacataagga tgcgggcggt aagccggagc tggcgcagga
     1501 gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac gctgactgaa
     1561 tcaggaacca caaccacttt gtatccactc ggcacatcga cggtgacact ttgcgccatc
     1621 gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt cataatcatt
     1681 tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca ttcaggcatt
     1741 gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt taagaacttt
     1801 atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt ttgccgctgc
     1861 gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac gcagcatctc
     1921 aatatcgtta ccgccatcgc caaagaccac aacttcgctg tcgtctattc cccataatt
//
LOCUS       DADKXM010000276         1948 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.279, whole genome shotgun sequence.
ACCESSION   DADKXM010000276 DADKXM010000000
VERSION     DADKXM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.279"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="JQR05_004101"
     CDS             complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="JQR05_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY5326051.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAYQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYCINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          620 a          336 c          307 g          685 t
ORIGIN      
        1 tccaggattc ccggggcgtt caccacatgc aatgtgttgt tgcaggggaa cggtctgcac
       61 catcccctat atcgatcaat ataacaaaaa tgacaacgtc agattcaggg cacaacctaa
      121 atatatttta ggtcacctcc caaatcgttt gcctgatacc gctccatttt ttaataaaaa
      181 atcaattgtt tttgaatctt tgcttttcat tgcattgtca agcatagtgt ccccactatt
      241 tgctttttga aatcttttgt ttacatcaac caatccatta ttaatacaat attctagcac
      301 tttatagttg gcactatctt cactaagtaa atactcaaca tcatacagct cacaatacat
      361 attgttgttc attttctcca tctctgcgac atcttgtttt gtaaaattaa atttagatag
      421 taaataaagt gccatatcag attttgcatt gcctagagaa taagcaagag cttgataagc
      481 cattttttta tcagtcacct tattgttaat ttcttcataa ttggatgctt taatggcatc
      541 ctccaaagat atatctctga gtcttatctt tttgaagtta tctgtgctta gcattcttgg
      601 tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa caggatcaat
      661 gtttttatta taacaaaact ctcggaatct ttgatcttta ctctttctta taaaatcaat
      721 aagatgcaac ccaattccaa gcctcatatc tttagtatta aatattggca catcagtttt
      781 tccctgatta ccatgcacat acctgctaac ttcatccaca acctctttaa agttatctaa
      841 gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata gcattatcaa agtggtcggt
      901 taatgtcata tatccatatg gagagtcatg atcaatgata tacgcattag caccaaaatc
      961 cacagtatga tgtgttttat ttagtgggag tgtttcttga tgattggaaa tttcaacccc
     1021 aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc tcagagaatg tgtttttata
     1081 tgtttgtata ttctcagact taattttatc aaaggactta atattaagag tattatttga
     1141 caggttagcg ttactttgat gtctgaagtt aaaatcagaa ttaataataa tagagagcaa
     1201 gtgacgttca tccgaagaaa gaggatgagt tttttgtatt atttcagaaa gatagtgtct
     1261 ggacacctta tcgaggtgct caagtgaccg aaatgatgac cgtgctccag atggcgcttc
     1321 aatagaaaga tttaatgatt tgaaactggt cccttgagag aacattttac tataactttg
     1381 cgctgctata gacttcctta aaaagctttt cacagtatca gcacagtgat ctaaatgttt
     1441 ttcacttatg ccagaagagg atgagtttgt tacgcatgat atgttttttt gtaatgcttt
     1501 tgtatctttg ttcggattta tttttccatc aacaagatca atattgttct gaacattaat
     1561 atgatttact gcttcaggta ttttcataaa acatccccca ttatgtgcgt ttttatctgc
     1621 agttatttag aataatatta tctaacttaa tattttcttg tatgtattat aacgaacata
     1681 ttttaatata ttgtacgatt ttcactctga aatcccctga gtttaatcac acttccttta
     1741 ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg cgccttgtta
     1801 tgtagttaat gctcagaata tgatagataa aatagagacg cagaacaggg catgctcata
     1861 acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg cgctcaacaa
     1921 aaagagagct aaatagctgc gcggaata
//
LOCUS       DADKXM010000277         1934 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.280, whole genome shotgun sequence.
ACCESSION   DADKXM010000277 DADKXM010000000
VERSION     DADKXM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.280"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            411..1219
                     /locus_tag="JQR05_004102"
                     /pseudo
     CDS             411..1219
                     /locus_tag="JQR05_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(1240..1803)
                     /gene="smrA"
                     /locus_tag="JQR05_004103"
     CDS             complement(1240..1803)
                     /gene="smrA"
                     /locus_tag="JQR05_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY5326052.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKAAQAKQENWERHAKRSR"
BASE COUNT          482 a          496 c          464 g          492 t
ORIGIN      
        1 acttcaatac cctggactta ctcaccagtc ctgtctggat tgtttcgccc tttgaggaac
       61 agttaattta tgccaatagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc
      121 tacgaaccgg accctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc
      181 tgcaaaacca acacgatatt atcgaaatcc tcactgttca gcgtaaagaa gaggaaacag
      241 cattaagctg tcggcttgtt ttgcgagagc tgacagaaac agaaccggtg attattttcg
      301 aaggtatcga agcgccggca acgctgggtt taaaagccag tcgctcggca aattatcagc
      361 gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga
      421 ttgacccgtc acgagatgga caaatcgtcg atgttaacct caccgcgctc aatttctatg
      481 gttataacca tgaaacgatg tgccagaaac atacctggga aataaatatg cttgggcgtc
      541 gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg cggtcataac cctttgaatt
      601 ttgttcataa actggcggat ggatctactc gtcatgtgca gacctatgcc ggaccgattg
      661 aaatttatgg cgacaagctc atgttatgta ttgtgcatga tattactgag caaaaacggc
      721 tggaggagca gctggaacat gctgctcacc atgacgcgat gaccggatta ctgaatcggc
      781 gacagtttta tcacattacg gaaccaggcc aaatgcagca tctcgccatc gctcaggatt
      841 acagcttgtt gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt
      901 ctaaagggat gaggtgttat gcgccctcgc ccgtcccctc gaaagttgcg ctcgcaaagg
      961 cgatttggtg tttcgttggg gaggcgaaga gtttgtctta ttgctaccaa gaaccccact
     1021 ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc gtggcaaaag tgagtatttc
     1081 gggcttacca cgctttaccg tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat
     1141 cgatgaactg tttaaacgcg ttgatgatgc tttgtatcgg gcgaaaaatg atggacgcaa
     1201 ccgcgtgctg gcggcataag ccgcggatgc gtctcgagat caacgactgc gcttagcgtg
     1261 gcgctcccag ttttcttgct tcgcctgcgc cgctttacgt agtgcgacgt aacacgcccc
     1321 gctgccgcca tgatgcggta gcgcggtgca atatgcctga acatcatcaa attcggtcag
     1381 ccagcgcgcc acatagctgc ggacaatatt ggcatgtgat ttatcttccc gccctttacc
     1441 atgaataatc agcacattac gcaaaccatc cgccagggct tgttgaatga aactgaacac
     1501 cattttgcgg cactcttcca ccggctggcg caaaagattc aggctcgcct gttgcggata
     1561 tttaccactg cgcagcttat ccagcacccc atgctgcaac ccttcctgcc gaaactccag
     1621 cggctgactt agcgggatga tgtcgagaaa tccggtagtg aggaaattat caagctgcag
     1681 cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga tgccagtgga catctgtagc
     1741 acgtttcagc ggctggacat cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt
     1801 catgtaacct cctatgcacc ttcacacgtc gtgattcaga cgggttaatt gttcatactt
     1861 actgcatcgt tcccttcaca gattgaaaca atactctact tcatacctgt gagtatctgc
     1921 gcaggcgggg catt
//
LOCUS       DADKXM010000278         1931 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.281, whole genome shotgun sequence.
ACCESSION   DADKXM010000278 DADKXM010000000
VERSION     DADKXM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.281"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            62..706
                     /locus_tag="JQR05_004104"
     CDS             62..706
                     /locus_tag="JQR05_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326053.1"
                     /translation="MLYAARKRGLEIGIFCTIHTLRLHFEEHLPLVVAGRRLGVPKST
                     VCSMFVRFRKAGLSWPLPAGMSERELDARLYGSASTVPVVLTESTVMPEVPGVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNHGFDIIYMLSTHHQRFTFVRLFDPYLIGSRPTFSHLAHHHIS"
     gene            complement(800..1447)
                     /locus_tag="JQR05_004105"
                     /pseudo
     CDS             complement(800..1447)
                     /locus_tag="JQR05_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440616.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1446..1931
                     /locus_tag="JQR05_004106"
                     /pseudo
     CDS             1446..1931
                     /locus_tag="JQR05_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          417 a          561 c          506 g          447 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagaa
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgtg gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccatcaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cgctgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ccgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgtt
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tgataggcaa ttcaggtaat aaaaaagccg gagctccctt tcctgcacgt ctcattgccg
     1861 tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg actgctcagc gagaatcgtc
     1921 gaaaaggacg a
//
LOCUS       DADKXM010000279         1879 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.282, whole genome shotgun sequence.
ACCESSION   DADKXM010000279 DADKXM010000000
VERSION     DADKXM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.282"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..263)
                     /locus_tag="JQR05_004107"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JQR05_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /locus_tag="JQR05_004108"
     CDS             complement(378..1016)
                     /locus_tag="JQR05_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326054.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="JQR05_004109"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="JQR05_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          544 a          461 c          351 g          523 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg gctaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa ttacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgagtaag
     1741 cgtacagcct gaaccgtctg gtcagaatct gacgaattag acaaagtggt gtccaccaaa
     1801 taagtagtgg gaaccaaagt gtcagatatg cagaaaaatg tgactcccgg caggcgaaaa
     1861 ggctgcccta attatcctc
//
LOCUS       DADKXM010000280         1863 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.283, whole genome shotgun sequence.
ACCESSION   DADKXM010000280 DADKXM010000000
VERSION     DADKXM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.283"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JQR05_004110"
     CDS             <1..246
                     /locus_tag="JQR05_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326055.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JQR05_004111"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JQR05_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY5326056.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JQR05_004112"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JQR05_004112"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY5326057.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
BASE COUNT          514 a          468 c          464 g          417 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtcgga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaa
//
LOCUS       DADKXM010000281         1834 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.284, whole genome shotgun sequence.
ACCESSION   DADKXM010000281 DADKXM010000000
VERSION     DADKXM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.284"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            209..997
                     /gene="virF"
                     /locus_tag="JQR05_004113"
     CDS             209..997
                     /gene="virF"
                     /locus_tag="JQR05_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY5326058.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(939..1260)
                     /locus_tag="JQR05_004114"
                     /pseudo
     CDS             complement(939..1260)
                     /locus_tag="JQR05_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1361..>1834
                     /locus_tag="JQR05_004115"
     CDS             1361..>1834
                     /locus_tag="JQR05_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326059.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRPQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRL"
BASE COUNT          576 a          329 c          368 g          561 t
ORIGIN      
        1 tccctttcag tgcaaatact tagcttgttg cacagagaaa tagaagctgc ataagctctt
       61 tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt acttaatcta tcttaataac
      121 ggaaagattt tgtatacaat cactgttaca gaaatttctt agttactctg taaacactaa
      181 atatagtttg gtatattctg ttgaatttat gatggatatg ggacataaaa acaaaataga
      241 tataaaggtt cgcttgcata actatattat tttatatgca aaaaggtgtt caatgacggt
      301 tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta tagagcgaaa
      361 tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg agattataag
      421 ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt attcatttca
      481 acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc tctctgagga
      541 ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca aaagaaagat
      601 ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat atacttcgat
      661 atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg aaaaaaacat
      721 cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag aaatagctgt
      781 tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg atattcgcat
      841 gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg tatcaagact
      901 tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt atggtataac
      961 tccaaagaaa ttttacttat atcataaaaa attttaaatg cttcatagcc catcgctatt
     1021 gccagatggg ttttccccac acctgatggc cccagtaata cgatattttc attacgttct
     1081 atgaagctga gtgagcgtaa cgactggagt tgcttctgcg gtgctctggt ggcgaatgtg
     1141 aagtcatact cttcgaacgt tttcaccgcc gggaaggctg ccattcgggt atacatcgcc
     1201 tgtttacgtt gatgacgtgc cagtttttct tcatgaagca tgatgctcca ggaagtccat
     1261 ataactccat tcctggtcta ctgcctgttg tgacagcgca ggcgctgcgc ttatgtactg
     1321 cacccatttt gttggacgat gaaatggaat agcccctgat atgtcaaagc caaaataccc
     1381 ttttgaaaag cgccttgaag tcgtgaatca ctacttcact actgatgatg gttacaggat
     1441 catctcggca cgttttggtg tcccccgacc ccaggtcagg acatgggttg ctctctatga
     1501 aaaacatgga gaaaaaggct taattcccaa acctaaaggt gttagtgctg atccagagtt
     1561 gcgtattaag gtcgtgaaag ctgtgattga gcagcacatg tcccttaatc aggctgctgc
     1621 tcactttatg cttgctggta gtggttctgt agccaggtgg ctgaaggttt atgaagagcg
     1681 cggagaagct ggtttacgcg cgctcaagat tggcaccaaa agaaacattg caatatcagt
     1741 tgatccagaa aaagcggcat cagcattgga gctgtcaaaa gaccgacgca ttgaggatct
     1801 tgaaaggcaa gttcgatttc ttgaaacgcg gctt
//
LOCUS       DADKXM010000282         1748 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.285, whole genome shotgun sequence.
ACCESSION   DADKXM010000282 DADKXM010000000
VERSION     DADKXM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.285"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..849)
                     /locus_tag="JQR05_004116"
     CDS             complement(<1..849)
                     /locus_tag="JQR05_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5326060.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIHSLTPFSLPMDLTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
     gene            complement(1238..1504)
                     /gene="ospE2"
                     /locus_tag="JQR05_004117"
     CDS             complement(1238..1504)
                     /gene="ospE2"
                     /locus_tag="JQR05_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY5326061.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1613..1748)
                     /locus_tag="JQR05_004118"
                     /pseudo
     CDS             complement(1613..1748)
                     /locus_tag="JQR05_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          498 a          280 c          362 g          608 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacgtctcg ggtaaggcag gtaagctttc
      481 cagttgatta aattgagcat gtattacctt aagcattggt ggtaaatcag ggagatgtgt
      541 taataggttt tttcttattt ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt
      601 taaatctaac tcagataaat ttaattctgc ctcttgattt tgtaaacata ttttcagcct
      661 attgaaggcg atattgcgtt gctcgcctgg ggtaccgttc ttttcccatt cactccaggc
      721 ttttaaataa aattcattac aagatggagt cagatccata ggtaaagaaa atggagttaa
      781 cgagtgtata ttgtttattt gatgcataat accagaaccg attgcctgta tattggttga
      841 tttaatcatt ataactagaa ttcttatcaa cgataagaat tctagtgaaa tagaatatag
      901 cttattaatt ttaaaacagt ttgctttgta ccctttctcc gggtattgct ttccccctaa
      961 gccgtctatg ggaaggagtc tgctgttatt cctgagaatg gtaattcagc cataaaaagg
     1021 tatttaaggt cattgcgtct ctccctgtac tcgaataatg gtgaagaaag catgatgaat
     1081 atatcacgat aacgttcagt acgtatcgag gataaatgga gttactcctc tctgaaatag
     1141 aattactgct ctattttttt ctgttttcag aagttattag taacaaatcc tgatatattt
     1201 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gaaatcagaa taccgttggc
     1261 tttgtttttc atcaattccg gcttcattta tctttgtcaa ccattgcatt tctgtagcag
     1321 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
     1381 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
     1441 atacttcaag gatgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
     1501 gcatattact tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg
     1561 ctaaatgaag tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga
     1621 atatgtgttt ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa
     1681 gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc
     1741 aagatata
//
LOCUS       DADKXM010000283         1710 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.286, whole genome shotgun sequence.
ACCESSION   DADKXM010000283 DADKXM010000000
VERSION     DADKXM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.286"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            265..534
                     /locus_tag="JQR05_004119"
                     /pseudo
     CDS             265..534
                     /locus_tag="JQR05_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            786..1376
                     /gene="ospG"
                     /locus_tag="JQR05_004120"
     CDS             786..1376
                     /gene="ospG"
                     /locus_tag="JQR05_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY5326062.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          501 a          358 c          402 g          449 t
ORIGIN      
        1 gacgaggtac tggccctgcg attgtctgaa aacggctcac aactgcacca ttcataatca
       61 catcgcataa aactgtcagc gccagtgata ggacaggtga aacctgtcaa tacgtaccgg
      121 cttacgccgg gcttcggcgg ttttactcct ctgccatatg caacaacgga gtgccgcctt
      181 acatgccgct ggcgcggcat atcctggtga cgatatctga acagttatat actgtgtata
      241 tacgtggtaa tgacgaggtg gcaaatggca caggttaata tgagtgtaag aatcgacgca
      301 gaactgaagg atgcttgtat ggccgctgca aaaagcatgg accgtaatgg ctctcagtta
      361 atccgggatt ttatgcgcca gaccgttgaa cggcagcaca attcttggtt ccgtgaccag
      421 gttgcggcag gccgtcagta actcgaatgc ggtgatgtgc ttccccatga catggtcgaa
      481 tcctctgctg ctgcatggcg ggatgaaatg agcaggaaga ttgctgataa atgatggaga
      541 tgttctggac catgctggcc atccaggaca ggaagcgcat tcgtagtaca ttgcggagca
      601 gaacctgatg gccgccattg aaccggacga acggattggt tattcagcca gcagcctggc
      661 agggcagccg tataaaggcc gcaacggtcg tgtggaagga acatctggac cacacaaggt
      721 agcgtgtaac gttattttgt gtgaaaatct cttgtgattt ttaaatatcg taacaaaggt
      781 atgacatgaa aataacatct accattattc aaacaccttt tccatttgag aataataatt
      841 ctcatgctgg catagtaacg gagcccattc tcggtaagtt aataggtcag gggtcgacag
      901 cagaaatctt tgaagatgtg aatgattcat ctgctttgta taaaaagtat gatcttattg
      961 gcaaccagta caatgagatt ctggaaatgg cttggcaaga atctgagctt tttaatgctt
     1021 tttatggcga tgaagcatcc gttgttatac agtatggcgg agatgtgtac ctccgaatgc
     1081 tgcgcgtgcc tgggactccc cttagtgaca ttgatacagc tgatatccct gataatatag
     1141 agagccttta tctacagttg atatgtaaat tgaatgagtt gagtataatc cattacgatc
     1201 ttaatacagg taatatgctg tatgataaag aaagtgaaag tttattccca atagattttc
     1261 gcaatattta tgctgaatat tacgctgcaa ccaaaaaaga taaagagatt atcgaccgac
     1321 gattacaaat gcgtacaaat gatttttatt cgttattaaa caggaaatat ttatagccgt
     1381 atttgttgat gctataagcc agaggctctt ctatgagaat atggggacca ttccctcgcg
     1441 atattaacgt aatatcttaa ttgtatgcac cgccagaatg cgttcagtgc gatcacggca
     1501 gcagacaggt aaaaatagca acaaaccacc cgaaaaaccg ccgcgatcgc gtatgataaa
     1561 ttttaaccac atgcatacct gtgcagcaag gcgaatcacg cacggatttc cagccagaga
     1621 cacctgaaac gtaaccggct catttaaacc gtctggtctg ttacctccgg ttccacaaaa
     1681 ataatgtcca tcattttgaa gtggacacta
//
LOCUS       DADKXM010000284         1663 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.287, whole genome shotgun sequence.
ACCESSION   DADKXM010000284 DADKXM010000000
VERSION     DADKXM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.287"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(118..723)
                     /locus_tag="JQR05_004121"
     CDS             complement(118..723)
                     /locus_tag="JQR05_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY5326063.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(894..>1663)
                     /locus_tag="JQR05_004122"
     CDS             complement(894..>1663)
                     /locus_tag="JQR05_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY5326064.1"
                     /translation="LQRLLTRFNDPEGGSNLAKNQYLSTSMKQKIWQRALSHRKNNPK
                     ADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVL
                     KELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWW
                     STLAKSNLTFFKFGALSNRHTPPAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPL
                     LALELVNPELDLVRQLALNGKTRAIREQAMKKLDELY"
BASE COUNT          487 a          405 c          348 g          423 t
ORIGIN      
        1 gaatacgtaa tgtaacttca tccccttttc tctcaggtaa ccgccatagc agaaaacggt
       61 ttatcagcgt tgctaaaatt aacaacgcga aaagtgttgc cagagatttt tccagcatta
      121 tctttcagcg ctcataagaa gcacactccg acaacgatta atcgcagtac agataagcag
      181 aattgaagca atgctccata aaagattatt ccattgcatc cactgtggat aaatggcaac
      241 ggccagaccc cacagaccaa atattaacgc gcgatcactt ttgccaaatg gaccggcata
      301 actgcgaaca ccgttaatcg tctgcgcgag taaaccgcaa aactcggtca atatggtgca
      361 aaataacatg agtatcacga gtgatgcgtt actttccggt aaaaataaaa acggtaaata
      421 gagcgcaata tcggaaataa catcgccagt ctcattcaaa atcgccccca gacgtgtttg
      481 ctgattgcac tcacgcgcca acatgccatc cagcgcattg agcgccatac ggataaaaag
      541 cacgatgggc aatagcaaaa agaggatggg ttgtgccacc aacaccaaca gcaatccggt
      601 aaaaagagaa agcgccagtg ccgtaagagt gatgtgattc gctgtaacgt ggtgcttata
      661 aagccaaaac atcgtcggcc ttaacagcga ctgaaagagc ggttttatct ggtagagcgt
      721 cattatcaat ccttgataat tcggtttgcg ctatggtgca tcaattcccc tgacggagat
      781 atcgcaacac gccgcttcat taaaaattca gaatatatca aggtattggg ggctcactgt
      841 caggaatgat tatccccaat aaatcaagta gttaaaaatt attacgctgt tattcaatac
      901 aactcatcaa gtttcttcat cgcttgttcc cgtattgcgc gcgtcttacc gttaagcgcc
      961 agctggcgga ctaaatccag ttcagggtta acaagctcta acgccagcaa aggatcacgt
     1021 ttcagcctcg ccttcaatac atcaactgga aagcggggat tattcatcgc cacaatccac
     1081 cactcgggat caatttctgc cgccagtgcc gcaggggggg tgtggcggtt gctaagtgcg
     1141 ccgaacttga aaaatgtgag attgcttttc gccagtgtac tccaccatat acgatccggt
     1201 tgttttgcca gtacatccag ttgtgctaac gcatcggggt cgagtgcata ataaacacgt
     1261 aatgcttcat caacctgcgc acgggagcta ttttcctgcc tcagaaaaaa cttcgcccaa
     1321 atatcattgc gtggcgtgtt ttgctgtaat tccttgagaa caacagcggg taaattatcc
     1381 caactaacta atgcactctc taaaaagtcc cagtcttcca aacgtatcag ggcggcggca
     1441 tttaatagca tctggtcatc aacttcgccg tggctaatca gctcagacaa aatcatatca
     1501 gcgctggttt catatgcatc tgaatctgct ttcggattat ttttccgatg cgataaagca
     1561 cgttgccaaa ttttttgttt catcgacgtg ctcagatact gatttttcgc cagattactc
     1621 cccccctcag gatcgttgaa acgtgtcaat aaccgttgta aag
//
LOCUS       DADKXM010000285         1637 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.288, whole genome shotgun sequence.
ACCESSION   DADKXM010000285 DADKXM010000000
VERSION     DADKXM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.288"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..1007)
                     /locus_tag="JQR05_004123"
                     /pseudo
     CDS             complement(1..1007)
                     /locus_tag="JQR05_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1342..1560)
                     /locus_tag="JQR05_004124"
     CDS             complement(1342..1560)
                     /locus_tag="JQR05_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326065.1"
                     /translation="MFKCNCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
BASE COUNT          448 a          391 c          348 g          450 t
ORIGIN      
        1 acgcactgct tttgtactgg aaagtgagat ttttagacag aacgcgcttc gcctgtaggc
       61 tgaagatata ccccagttcc tcttcagaat ggtgcacatc aagatgagca ttatcagtag
      121 tacgaggcga cgtagcgaac cggttgttat aggcttcaat aaaggtcggc aaccatgcat
      181 ttgctgtttc aatatcactg atattctgaa gccgcatttc tttgaccagc ctgtcctgta
      241 gtgtctgatt ggcgcgctct acccgccctt ttgcctgcgg gctgttggca tggattggct
      301 cgatgcccag tgtctttatc gcacgagtga actgggtcag ctcaccttcc cgctctgggt
      361 tatttaccct gaatatactg tgtctatcag agtagagagc gagcggtacg ccatgatcat
      421 taaggtaacc ccggagggtt tccatgtaag cccgggttgt ttcagcaggc acaaaacgca
      481 acgccatcaa cgcactggtg gcatcatcaa tgaaaacgat cagtgtacat ctggggcctc
      541 gattttcaaa ccagtcatga ggtgagccat caatctggat cagttcaccg taagatggtc
      601 gtcgcatgcg gcgttgatat atacgggcaa ttttacgacg gcgttcacgc cataccccct
      661 cttctatcat ccactttctg agagtttcaa cggataaaga taaaccgtgt atctcgcgca
      721 atttttcgca cgcaaacgta ggtccaaaat cagcgtaacg gcctttgagg agtgaaatta
      781 ctgttgctct gaattcagta gaaaaggaat tattaggacg ctttccacgt cggtgggaaa
      841 ccagaccaga aggcccttca tttctatacc gttgcaccag acgtttaact agaaatgcca
      901 atgcgtgccg cagcttgttc ctgagtaata tgtcgattaa ttgactcctg aataatctga
      961 agtcggtgga gttccttatg actcatcgta acagtctctt tgatcatgaa aaatccccca
     1021 gagaattgtc tggggacatt ttagaatggt tcaaagggga cattacagct tggtgttaac
     1081 aatagcctgc ttctccttgc ctttcggcac gtaagaggct aacctacatg tgtctggcat
     1141 gaaattggcc tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta actggggctt
     1201 ttctctacct gccgttggtg ttcgtgcccg aggcagatag cctcaagcac ccgcagtcat
     1261 tctacttaac taagatttcc ccgcaaaccg tttttgtcca gcacagtaaa tatccaacta
     1321 aaccaatgga gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
     1381 ccctaaaaaa agggcattta tatatccaaa tattaatatc aaaacatcaa ctttttccat
     1441 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1501 aaactgcaat gcgtttttca ttattcctcc tacaatcaat gtgcaattac atttaaacac
     1561 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1621 caaactcttt agtctaa
//
LOCUS       DADKXM010000286         1630 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.289, whole genome shotgun sequence.
ACCESSION   DADKXM010000286 DADKXM010000000
VERSION     DADKXM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.289"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..1046)
                     /locus_tag="JQR05_004125"
     CDS             complement(<1..1046)
                     /locus_tag="JQR05_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY5326066.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1538..>1630
                     /locus_tag="JQR05_004126"
     CDS             1538..>1630
                     /locus_tag="JQR05_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY5326067.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIV"
BASE COUNT          480 a          271 c          346 g          533 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacaatacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct ccccccccga
     1501 cttttgacgc agtgtcttgt taacaggggg atttaccatg cttattggct atgtacgcgt
     1561 atcaacaaat gaccagaata ccgatctaca acgtaatgcg tttatttgtg ccggaagtga
     1621 gttgattgtt
//
LOCUS       DADKXM010000287         1617 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.290, whole genome shotgun sequence.
ACCESSION   DADKXM010000287 DADKXM010000000
VERSION     DADKXM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.290"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(820..1044)
                     /locus_tag="JQR05_004127"
     CDS             complement(820..1044)
                     /locus_tag="JQR05_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY5326068.1"
                     /translation="MISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQRGRST
                     AVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1253..1617)
                     /locus_tag="JQR05_004128"
                     /pseudo
     CDS             complement(1253..1617)
                     /locus_tag="JQR05_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          364 a          451 c          383 g          419 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacataac ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gaggggcccc aattaagttg tcaagcatgt ttactgcgca atcgcttggt
      421 gctccgtccc tgtaccccgc attggtattc tggcgctcat ggggcttcca cttgcgcttc
      481 tcccttcaca tcggggtgac aggtccacgt tccccacaag agcctgaatc gacttcacgc
      541 cgcttttatg ccggatgccg cgcaggcagt aaacatatcc cacaggaacg actccaccat
      601 tgttttgaca tcatctatat gctttcgaca catcatcagc ggttcacttt cgttcgtctc
      661 ttcgaaccat acctgatcgg ctcacgtccg accttttccc atctcgctca ccaccacatc
      721 tcttaaatgc agcagcagtg gatggtttgg tgcctgcgcc tgcacgccga caccgagggg
      781 ccttccctca tctcttgtgc cgcatggctg cacagggact cacgtcccca ggacgcgcct
      841 tcgtggcaca cagtcatcgg catagcaaca gtaagcaacg gcaggtttcc attgccagtt
      901 ttctttgaca gcagtacttc ggcctcgctg gatactgtga ttccagtacc agcgatcctt
      961 tctggctttt ccactcaggt agcgtttatt cagatactgg tcgaactcat tcagtatgat
     1021 gttcgacagc aacggcgaga tcacaccgcc ctgtgcatac tttcactgac cgcccggaag
     1081 agaccaacat caacacggcg ttttccacag cagtctcata aaacgtgcat cactgatcct
     1141 gcggcagacg gctttcatca gcagccggtg atgtactgtg tcgaagtagc tggacaggtc
     1201 accttcaata acccggcgcc ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga
     1261 tgtctcccgc ccacttctgg ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt
     1321 ctgatacagg caggccgtgt gcacggtaac tgatcgggct gaacttctgg gaggcttttg
     1381 cgctcagttc ctgacggcgc agactggctg ccacggtttt tacgttaaag tggtaaccag
     1441 agcacacagt tcatccgtca ggcgtggggc accgtaacgc tggactgggt aaaagccttg
     1501 cggacaacgc tgtcgcagtg ctgacggaac tgctgacgtg tgtttatcct tgtccgccgc
     1561 tgacaccacg tataccagcc gctgcgggcc acccggagta cgcggcacat tgccttg
//
LOCUS       DADKXM010000288         1596 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.291, whole genome shotgun sequence.
ACCESSION   DADKXM010000288 DADKXM010000000
VERSION     DADKXM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.291"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            3..386
                     /locus_tag="JQR05_004129"
     CDS             3..386
                     /locus_tag="JQR05_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5326069.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(387..1295)
                     /locus_tag="JQR05_004130"
     CDS             complement(387..1295)
                     /locus_tag="JQR05_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5326070.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            1395..>1596
                     /locus_tag="JQR05_004131"
     CDS             1395..>1596
                     /locus_tag="JQR05_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY5326071.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
BASE COUNT          423 a          354 c          381 g          438 t
ORIGIN      
        1 tggtgctggt ggcaattctg attatgctgc cgactctcgg catcggtgat atgaacaatc
       61 tctataaatg gctgttgaat cttaactcgg tagtgatgcc gctgcgttat ttatgggtat
      121 ttgttgcatt tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta
      181 ttcgtaataa gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctgtg
      241 cctgtttgac ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt
      301 tggcgctgaa tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc
      361 tggcgcgtaa agcgaatagc aaataattat tgtggtcggt gacgctgact ctggcagatc
      421 tgacccgttt ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt
      481 gggtaataga cttggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa
      541 cgaccagtat caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc
      601 atcaaaacgg cctccagttc agcatcaaca tcatccagaa tgatatttcc tgcgacagca
      661 atttccagct cctttgcaaa ttgccagtgg aaaggtttgc cgctgggata acgaaaaaca
      721 acacattgat gattaagcag atcatgagga tggcggggtt tgccatatct tgcaaagtac
      781 tctggcgttg ctgctacaca taatttcact ggtgggccga tcgccacgct gatcatatct
      841 ttttcgacga tgccactaag tcgaacgcca gcgtcaaagc cctgttggac gatatcgata
      901 agagaatcgt cagttgtgag ctctacttta atatcaggat attcacgtgt gaaaccaacc
      961 agtaaaggca ttaaaaatat acgtgcagct acccgggcgg cattgatttt gagcgttcct
     1021 gtcggtgtca gacgaaaatc attcatctca tctaacatga tctgaatttc gtcgaaagct
     1081 gggcgcaatc gttcatagag attagaaccc gcttccgtta acgatacgct tcgggttgtc
     1141 cgattgaata agcgaatttt aagacgttgt tccagtgttt taatactatg gctaatggcg
     1201 gacgaggata agcctaactc atcgcctgct gcacgaaagc tttgattacg ggccacggca
     1261 aagaaagtgg caaagtcaga catattcatc cgcattagtg aaaatccttc attaactcat
     1321 cctgaatcat cacgtttaca ggattatgcc gcctgcgtaa agtgcgctcc agaacttaac
     1381 gtggaggtaa aattatgcag tctgaacgta tttatttggt atgggcccat cctcgtcatg
     1441 attcattgac cgcaaatatt gctgatgcga tccatcagcg ggcaatggag cggaaaatac
     1501 aggtgacgga actcgattta tatcggcgta atttcaaccc ggtgatgacg tcggaagatg
     1561 aaccagactg gaagaatatg gataaacgtt attctc
//
LOCUS       DADKXM010000289         1587 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.292, whole genome shotgun sequence.
ACCESSION   DADKXM010000289 DADKXM010000000
VERSION     DADKXM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.292"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..398)
                     /locus_tag="JQR05_004132"
                     /pseudo
     CDS             complement(1..398)
                     /locus_tag="JQR05_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(373..1173)
                     /gene="otsB"
                     /locus_tag="JQR05_004133"
     CDS             complement(373..1173)
                     /gene="otsB"
                     /locus_tag="JQR05_004133"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY5326072.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(1340..>1587)
                     /gene="araH"
                     /locus_tag="JQR05_004134"
     CDS             complement(1340..>1587)
                     /gene="araH"
                     /locus_tag="JQR05_004134"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY5326073.1"
                     /translation="PMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVEN
                     AMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT          385 a          425 c          377 g          400 t
ORIGIN      
        1 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
       61 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
      121 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      181 aaggtcctgt tcgctgagtt aaaagaggcc cacgtaatgt tacctttttt cacctttttt
      241 agcggctgat cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgcggct
      301 ttcagtgccc ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt
      361 ggtgcaatcc ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc
      421 tcttttttgt tgtaatgcgg tggttatcat ttcaagccag ctccagacat ccggcacacc
      481 cgccagtcgc catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag
      541 tcggttaacg actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt
      601 tcgcccgata aaaggagctt cctgcataaa ggctgcaatc gcttcacctt tactggtacc
      661 tctcggtttg atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg
      721 agtaatacgt tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg
      781 cagcgcaaaa gccatccctt tcgcctccag ctccgcgccg ggatactgag cgatgaccgt
      841 atgcagttgc acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgtgtttt
      901 accattgatg tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt
      961 tgccagtgcg tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact
     1021 tgcggttgcc agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg
     1081 atgcggtttg atttccgcca gcgttccatc aagatcaaaa aaccaggcat atttcgcgga
     1141 tagttcaggg gtttcggtta acggttctgt cacccggctc tcctccttct cttttcttat
     1201 tgtgttgcgc agaccgcgct ttacagacat gtaagtatag acagtgtgac ggaggtcgcc
     1261 atttgcaacg aagaacagcc aaccgttgtg gcggctggct gtggtgggaa aaaacgttaa
     1321 attgttgtgg aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga
     1381 tcaccgctgc cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa
     1441 gcaggttcat ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga
     1501 tttttccgat gccacctttc agagaaacgc cacctaaaac gcaggcggag ataacaataa
     1561 gctcataacc aatcgacgtc attggct
//
LOCUS       DADKXM010000290         1577 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.293, whole genome shotgun sequence.
ACCESSION   DADKXM010000290 DADKXM010000000
VERSION     DADKXM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.293"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..780)
                     /locus_tag="JQR05_004135"
     CDS             complement(<1..780)
                     /locus_tag="JQR05_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5326074.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISATGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
     gene            complement(1381..>1577)
                     /locus_tag="JQR05_004136"
     CDS             complement(1381..>1577)
                     /locus_tag="JQR05_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY5326075.1"
                     /translation="AVDALKEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKDSAK
                     TFVQETLKDTWESADEWFVH"
BASE COUNT          415 a          267 c          377 g          518 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct gttgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta atgttgtcag gcctaattca gacaagtcca ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt tattcttctg
     1141 gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt gttttgctga
     1201 gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca tgattggcgg
     1261 gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc ggctggctgg
     1321 tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt tacgtgctaa
     1381 ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt cctgaacaaa
     1441 agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt tgctggtggc
     1501 ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta attcttcctt
     1561 taacgcatcc acagcac
//
LOCUS       DADKXM010000291         1575 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.294, whole genome shotgun sequence.
ACCESSION   DADKXM010000291 DADKXM010000000
VERSION     DADKXM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.294"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..148)
                     /locus_tag="JQR05_004137"
                     /pseudo
     CDS             complement(1..148)
                     /locus_tag="JQR05_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            511..1260
                     /locus_tag="JQR05_004138"
     CDS             511..1260
                     /locus_tag="JQR05_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY5326076.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1430..>1575)
                     /locus_tag="JQR05_004139"
     CDS             complement(1430..>1575)
                     /locus_tag="JQR05_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326077.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT          426 a          337 c          334 g          478 t
ORIGIN      
        1 atgttttcag accaaactgc tcctgtatga cccggtaatc gtatgcgcag tactgtgaac
       61 ctcgatcgga gtggtggatt agcccggcag gtgggcgctg gctcctgagc accataaaca
      121 gggctttacc cgtcaatatt ggctccttct gatgccattc tatttcagga aggagtgtcc
      181 gttaaactca ggctacctca aaaagttatc ggtgcgttta tcgaaaaaca tatgttctac
      241 taattggagt cattgtctgt aaaagcaact caaaaataca agttgcttat tccccttgtt
      301 ggtaagaact taattctaac tgtttgttct gggtgtttta aacgtcctcc tttttatgct
      361 tgttcataac ggatatttaa taggtaacct ttgttttagc atcttctgat ttatttaaat
      421 tatgtgatag cgcacacaat tttattatcc agtactttat actcttaata aaataattat
      481 aaatatttat cctctataag gagatgttat atgaaaagac agctttttac tcttagtatt
      541 gtcggggtat tttctttaaa tacctttgca tcattccctc cgggaaatga tgtgacaaca
      601 aaacctgacc tttactacct gacaaacgat aatgctattg acagtctggc attattaccg
      661 cctccaccac aaatcggaag tattgccttc ctgaatgacc aggccatgta tgaaaagggg
      721 cgtttattgc gaaatactga aagagggaag ctggcagctg aggatgccaa tctcagtagt
      781 ggtggcgtgg caaatgtatt ttctgctgct tttggttctc cgataacggc taaagattca
      841 ccagaattgc ataaattact gacaaatatg attgaggatg ccggagacct ggcaacgcgt
      901 tcagcaaaag aatattatat gcgtattcga ccttttgctt tttatggtgt ttctacgtgt
      961 aatacaaaag aacaggatac attatccaga aatggctctt atccatcagg tcatacatcg
     1021 attggttggg caacagcact tgtattgtcc gaaataaatc cggcacgcca ggataccatt
     1081 ctgaaacggg gttatgaact gggggacagc agggttattt gcggttatca ctggcaaagt
     1141 gatgttgacg cagcacgtat tgttggctct gctattgtag caacactgca ctcaaatcct
     1201 gtgttccagg cacaattaca aaaagcgaaa gatgaattcg ctaacaatca gaaaaaataa
     1261 tgcttttagc cagtacgcct tataaccccg accttcaggg ctaatgccag tcagttaagc
     1321 aactgactgg ctctttttcg gggctgtggg gtatttccag ggcctctcct ttaccactct
     1381 cgggaaggcc ctttcccttc ttgtcggtaa tttcactagg ctgtgtccct taattacgta
     1441 agcgttgata aaagagtcgg atgcagccca gtttcaccat cgccaggtaa tttctcgccg
     1501 ttttgtcgta acgcgtggcg atgcggcgat attctttcag ccgcccaaag caccgttcaa
     1561 cgacattacg attgc
//
LOCUS       DADKXM010000292         1571 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.295, whole genome shotgun sequence.
ACCESSION   DADKXM010000292 DADKXM010000000
VERSION     DADKXM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.295"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..120)
                     /locus_tag="JQR05_004140"
     CDS             complement(<1..120)
                     /locus_tag="JQR05_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326078.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINS"
     gene            complement(146..1477)
                     /gene="tdcC"
                     /locus_tag="JQR05_004141"
     CDS             complement(146..1477)
                     /gene="tdcC"
                     /locus_tag="JQR05_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY5326079.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
BASE COUNT          487 a          382 c          378 g          324 t
ORIGIN      
        1 cgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg catcgagtac
       61 ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa attcattcat
      121 ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc aggatggtca
      181 gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc gccagagacg
      241 gcgctttacg gatagcatac atcggcaaca ggcacagcag ggatgcgata atcggtgcgc
      301 ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc caggtggagc
      361 ccatgatgaa gatcatgctg agagtattca gtttacccag cgacactttg gttttgtcac
      421 ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc agatagtgac
      481 cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat tccagtgtaa
      541 tcgcgaacgt tgttttcgta ccggtcatgg acgcaaagtg gttagccaga taagaaagca
      601 ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagagta aacaggcagc
      661 taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa atgatttggg
      721 aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca cgtttagaaa
      781 ccacaaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg gaaatcccca
      841 gccagacagt aatcaggata ccgtcatgac cggttaacga cagcgaaccg aggtcaacct
      901 ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc accaggctgg
      961 cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta ccaaaccaga
     1021 tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc agcggtgcaa
     1081 atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg ccataaatcc
     1141 acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg ccagttttac
     1201 caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca gaaagacaca
     1261 gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc aacatcagaa
     1321 gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc ccggcgccga
     1381 ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag gacgattgtt
     1441 ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa cgaattaatt
     1501 aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg ccgccggaaa
     1561 taatactgac g
//
LOCUS       DADKXM010000293         1549 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.296, whole genome shotgun sequence.
ACCESSION   DADKXM010000293 DADKXM010000000
VERSION     DADKXM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.296"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            351..476
                     /locus_tag="JQR05_004142"
     CDS             351..476
                     /locus_tag="JQR05_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAY5326080.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="JQR05_004143"
     CDS             complement(921..1397)
                     /locus_tag="JQR05_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5326081.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          406 c          385 g          399 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcgccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggca gcgctcaagg cgctgccgga
      781 aacgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcgacg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DADKXM010000294         1478 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.297, whole genome shotgun sequence.
ACCESSION   DADKXM010000294 DADKXM010000000
VERSION     DADKXM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.297"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            49..1029
                     /locus_tag="JQR05_004144"
     CDS             49..1029
                     /locus_tag="JQR05_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY5326082.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          408 a          344 c          343 g          383 t
ORIGIN      
        1 taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat gaacgcaata
       61 atatcgcccg attattacta tgttcttacc gttgctggtc agtctaatgc catggcgtat
      121 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat taaacaatta
      181 gcgagatttg cgcatacgca tccaggaggc ccgccatgtc actttaacga cattattcca
      241 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
      301 aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg
      361 cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg cggatcggct
      421 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgc
      481 cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg agccgcactg
      541 gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
      601 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
      661 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc gtggttttgc
      721 ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc tatttatggc
      781 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
      841 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
      901 tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca tttcagcacg
      961 gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca gttttggcgc
     1021 gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca gagtagacaa
     1081 caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac agcaggcaat
     1141 tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac cgttgttatt
     1201 gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta tggctcggaa
     1261 atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc gcagcaccgt
     1321 cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga caatgatgat
     1381 atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc attttctttc
     1441 attcctaaaa gcacccgcca ttaattccag gcgcatta
//
LOCUS       DADKXM010000295         1467 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.298, whole genome shotgun sequence.
ACCESSION   DADKXM010000295 DADKXM010000000
VERSION     DADKXM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.298"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(272..>1467)
                     /gene="ospC4"
                     /locus_tag="JQR05_004145"
     CDS             complement(272..>1467)
                     /gene="ospC4"
                     /locus_tag="JQR05_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY5326083.1"
                     /translation="SIEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLL
                     SIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFF
                     GVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQS
                     FFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFC
                     YNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNK
                     VTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLL
                     SKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILG
                     KRFEI"
BASE COUNT          459 a          260 c          244 g          504 t
ORIGIN      
        1 cccgtaaaga caggagtcaa gttcccgctc cgacataact gcgtgcaatg gattttgaag
       61 gaaccgtcag cccattcccc ggaggcaaac atgggctggc aaaagtgtag cggtattagg
      121 cacagctatt taggaaagag ggaccatctc catctcatta aatggaccac gccagacaat
      181 tcactgtcat gctgatgctg cactgtgggg acagaattac tcatgatgac ttcgataatc
      241 gacgacatta ttatttggcc gagcttttag gtcatatctc aaatcgtttg cctaatattg
      301 ctccattttt taataaaaaa tcaatcattt ttgaatcttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt gctttttgaa attttttgtt tacatcaacc aatccattat
      421 tgataaaata ttctagcact ttatagttgg caccatcttt actaagtaag tattcaacat
      481 catacagatt acaatatcta ttgttgttca ttttctccat ctctgcgaca tcttgttttg
      541 taaaattaaa tttagatagt aaataaagtg ctatatcagc ttttttattg cctaaagaat
      601 aagcaagggc ctgatgagcc atttttttat cagtcacttt attgttaatc tcttcataat
      661 tagatgcttt aatcgcatcc tccaaagata tgtctctgag ttttattttt ttgaagttat
      721 ctgtacttag cattcttggt atatgatact caagctgaaa cacaaagttt ataattctat
      781 ccagactaac aggatcaata tttttgttat aacaaaactc tctgaatcct tgatctttac
      841 tctttcttat aaaatcaata agatgcaacc caattccaag cctcatatct ttagtgttaa
      901 atattggaac atcagttttc ccctgattac catgcacata cctgctaact tcatccacaa
      961 cctctttaaa gttatctaag aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag
     1021 cattatcaaa gtgatcggtt aatgtcatat atccatatgg agagtcatga tcaatgatat
     1081 acgcattggc gccaaaatct acagtatgat gtgttttatt tagtgggagt ttttcttgat
     1141 gattggaaat ttcaacccca aaaaaaacaa aatcgtggtt agagatttct ttaatatcct
     1201 cagagtatgt attcttatgt gtttgtatat tttcagactg aattttatca aaggatttaa
     1261 tattaagaat attatttgac agattagagt tactttgatg tctgaagtta aaattagaat
     1321 taataataat agaaagtaag tgacgttcgt ctgaagaaag aggatgaact ttttgtatta
     1381 tctcagagat atagtgcctg gacaccttat cgagatgctc aagtgaccga aatgatgacc
     1441 gtgctccaga tggcgcttca atagaaa
//
LOCUS       DADKXM010000296         1421 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.299, whole genome shotgun sequence.
ACCESSION   DADKXM010000296 DADKXM010000000
VERSION     DADKXM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.299"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..1406
                     /locus_tag="JQR05_004146"
     rRNA            <1..1406
                     /locus_tag="JQR05_004146"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          321 c          440 g          302 t
ORIGIN      
        1 gccgaggcgt gatgacgagg cactacggtg ctgaagcaac aaatgccctg cttccaggaa
       61 aagcctctaa gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta
      121 gagaatacca aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa
      181 cttcgggaga aggcacgctg atacgtaggt gaagcgactt gctcgtggag ctgaaatcag
      241 tcgaagatac cagctggctg caactgttta ttaaaaacac agcactgtgc aaacacgaaa
      301 gtggacgtat acggtgtgac gcctgcccgg tgccggaagg ttaattgatg gggttagcgc
      361 aagcgaagct cttgatcgaa gccccggtaa acggcggccg taactataac ggtcctaagg
      421 tagcgaaatt ccttgtcggg taagttccga cctgcacgaa tggcgtaatg atggccaggc
      481 tgtctccacc cgagactcag tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc
      541 aagacggaaa gaccccgtga acctttacta tagcttgaca ctgaacattg agccttgatg
      601 tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa
      661 ataccaccct ttaatgtttg atgttctaac gtggacccgt gatccgggtt gcggacagtg
      721 tctggtgggt agtttgactg gggcggtctc ctcctaaaga gtaacggagg agcacgaagg
      781 ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca gcttgactgc
      841 gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg ttctgaatgg
      901 aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat accgcccaag
      961 agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc ctggggctga
     1021 agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct gggtttagaa
     1081 cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg gagaactgag gggggctgct
     1141 cctagtacga gaggaccgga gtggacgcat cactggtgtt cgggttgtca tgccaatggc
     1201 actgcccggt agctaaatgc ggaagagata agtgctgaaa gcatctaagc acgaaacttg
     1261 ccccgagatg agttctccct gaccctttaa gggtcctgaa ggaacgttga agacgacgac
     1321 gttgataggc cgggtgtgta agcgcagcga tgcgttgagc taaccggtac taatgaaccg
     1381 tgaggcttaa ccttacaacg ccgaagatgt tttggcggat t
//
LOCUS       DADKXM010000297         1413 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.300, whole genome shotgun sequence.
ACCESSION   DADKXM010000297 DADKXM010000000
VERSION     DADKXM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.300"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>1413
                     /locus_tag="JQR05_004147"
     rRNA            <1..>1413
                     /locus_tag="JQR05_004147"
                     /product="23S ribosomal RNA"
BASE COUNT          385 a          303 c          448 g          277 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gcgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tgctgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtt
//
LOCUS       DADKXM010000298         1399 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.301, whole genome shotgun sequence.
ACCESSION   DADKXM010000298 DADKXM010000000
VERSION     DADKXM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.301"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..441)
                     /locus_tag="JQR05_004148"
     CDS             complement(<1..441)
                     /locus_tag="JQR05_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY5326084.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEW"
     gene            complement(438..728)
                     /locus_tag="JQR05_004149"
     CDS             complement(438..728)
                     /locus_tag="JQR05_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY5326085.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(728..1327)
                     /locus_tag="JQR05_004150"
     CDS             complement(728..1327)
                     /locus_tag="JQR05_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY5326086.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          322 a          387 c          307 g          383 t
ORIGIN      
        1 ccactcaggg tgtgcgtcat tcagcaattc agccatttta cgcttagtca tcccccgccc
       61 ttcataccgt tgccggagga cactaatcaa tccaggatgc tctgccagca cctcacttat
      121 gactctatca atacataacg cctctgcatc agtacaatgc gccagccagg tcttttgctt
      181 gccattgatc atctctcgca aaaacgcttc cagctcaggt ttctctattc ccgctttttt
      241 catcctgcgc agggcttcat tgacggctgt tttcgtcaat tttttggatg ccagcaactg
      301 gttaaacata tttcccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag
      361 ttttccctga atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc
      421 tgtatttgtt gggtaaatca taaataacct tcctttctcc agatttcttg tgtgcgaaaa
      481 acaccttctg catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc
      541 ccgtcgatta aatcgtggca tgagctacag gcaatcgctg cctgcatatc gtgtggtttt
      601 gtcgctgttc cgcacgtccc cgccagcctg taatgcgcca gcacagaagt ttcgggattg
      661 tgattgcagt agccaggaat tctgacggtg cacatctgcc cccgcgccgc tttacgtaaa
      721 tccaccatta cgcaaactcc agtaactgcg cggccacatt ttcgacttcc tccggagagg
      781 aaaatttacg gaacaggatc cagttccaca gcacattcag tacagattta taaacctgct
      841 gaaactcggt ttcgtccatg ttcgcaaatg cgatagattt tgccctgcgc ccacgactac
      901 catcaggata aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag
      961 cgtcaaacga ctttagcaat gccgtatccc gggttctgca tgtcgcaact gtattaaggt
     1021 attgctctgc ggcatcactc agggctggag tgtgttcccg acctactgat tcgcacaggt
     1081 aatcaacgaa gcctgatacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
     1141 tccagtaatc aaatcccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
     1201 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc aaaaaatcgc
     1261 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
     1321 gcgccatcgt agttctccgc tggcgcagta gaatgggtgt tcagcccgtt atgtagtata
     1381 ccagaattaa tgccaatac
//
LOCUS       DADKXM010000299         1396 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.302, whole genome shotgun sequence.
ACCESSION   DADKXM010000299 DADKXM010000000
VERSION     DADKXM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.302"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            43..630
                     /locus_tag="JQR05_004151"
     CDS             43..630
                     /locus_tag="JQR05_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326087.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(692..976)
                     /locus_tag="JQR05_004152"
     CDS             complement(692..976)
                     /locus_tag="JQR05_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY5326088.1"
                     /translation="MEFPIQGEMVDIVAHGELGGDFSLVPDSYVTMGPKSIMAAKNLL
                     IIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELAFG"
     gene            complement(984..1379)
                     /locus_tag="JQR05_004153"
     CDS             complement(984..1379)
                     /locus_tag="JQR05_004153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326089.1"
                     /translation="MSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKP
                     WYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYREHDQKL
                     AREGGLDLVVLGLGADSFLWQFAEYDPFP"
BASE COUNT          359 a          322 c          331 g          384 t
ORIGIN      
        1 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
       61 ccgttacccc aattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat
      121 aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt
      181 tattgcgttg atgaagttga acatctctgt ccgttgtgta ttgcggacgg ttctgctgct
      241 gaaaagtttg caggtagttt tcaggatgat gccagcatag aaggtgttga atttgagtat
      301 gatgaagagg acgaatttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag
      361 ttggttgaac gcacgccagg ttatcatgga tggcaacagg aattctggct tgcgcattgt
      421 ggcgatttct gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat
      481 gaatttgcca accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct
      541 aaatgcctgc aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc
      601 aagctgagac tgtggggtga tttttcgtag ttatttaaat aatgagaaca ggccggagcg
      661 taattcatac atccggcctt atttcttcag cttaaccgaa cgctaattct gcggcagcgg
      721 ctttatcagc gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt
      781 cggtaaccgg accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa
      841 ttatcagtaa gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag
      901 gcactaacga aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct
      961 ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa
     1021 aatgaatctg cacctaatcc cagcaccacc aaatccaggc cgccttcacg cgccagtttc
     1081 tgatcatgct cgcggtagtt atcaatagtg agcttctgga tattctcttc tttgatcccc
     1141 gcaggggtga aaaacagatt acgcagattg gtaatcgtta cgccttctcc ctctttgccg
     1201 cgaaagggaa tttcatcgaa attatagaaa tagcagttat cgtaccaagg cttaccttta
     1261 accagggtgg tgaggtattc atacatgcct ttgggcgtgc taccggcggt aattgccagg
     1321 ttaacacgac gcgtcttcga catataacca agcagatggt gtgccgcgac acggctcatt
     1381 tcctggtaat cttcgg
//
LOCUS       DADKXM010000300         1385 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.303, whole genome shotgun sequence.
ACCESSION   DADKXM010000300 DADKXM010000000
VERSION     DADKXM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.303"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            155..1208
                     /locus_tag="JQR05_004154"
                     /pseudo
     CDS             155..1208
                     /locus_tag="JQR05_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
     gene            1284..>1385
                     /locus_tag="JQR05_004155"
     CDS             1284..>1385
                     /locus_tag="JQR05_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326090.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWV"
BASE COUNT          376 a          285 c          341 g          383 t
ORIGIN      
        1 ttgggtggct ggtcgttaaa aattttaact gcatttagcc aacttaaatt aatgaaaaaa
       61 tgttattaat cgttgagcta aagtcattag agatgctttg tccttaatgt aaccatatcg
      121 caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat
      181 gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac
      241 gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg
      301 tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc
      361 gcgtgctgat gtcgattatc tgtttgctca agttatcgtc catgagcaac gtgtcgatac
      421 cacgcctaac tgcggcaata tgctgtctgg cgtaggggca ttcgccattg aaaatggttt
      481 gattgcagcg acttcgccag ttacccgtgt acgtatccgc aacgtcaata cgggtacgtt
      541 catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat
      601 tgacggcagt accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgctggaa
      661 cgaaaaccgg aaaagttttc ccgactgata atcagattga ttattttgac gatgtcccgg
      721 tgacctgtat cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa
      781 caggttatga attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat
      841 ctatccggct acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc
      901 ctaaacctgt gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta
      961 tgccgcattc ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt
     1021 gtgcattgga aggcaccgtc acccgacaaa tcgtcccttc tgtaggatat ggcaatatca
     1081 atattgaaca ccccagtggt gcgctcgacg ttcatttaag taatgaaggt caggatgcca
     1141 cgacgttacg cgcatctgtt attcggacaa ccagaaaaat attttccggt gaagtttatc
     1201 ttccctgaaa aaattcgttg tcaggataag gacaatcaat aaaggacttc tgtatgagtc
     1261 atacagaaag aacaggattt taaatgaata agaaatcgtt atggaagcta attctgatat
     1321 tagcgatccc atgtattatt ggttttatgc cagctccggc aggattaagc gaactggcgt
     1381 gggtg
//
LOCUS       DADKXM010000301         1381 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.304, whole genome shotgun sequence.
ACCESSION   DADKXM010000301 DADKXM010000000
VERSION     DADKXM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.304"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..211
                     /locus_tag="JQR05_004156"
     CDS             <1..211
                     /locus_tag="JQR05_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY5326091.1"
                     /translation="GHTVLNPAVLPDGLTQPQYMDICMAMIRCVDAIYMLKGWQRSAG
                     AKAELALAEILGHAVIFQETASVQD"
     gene            198..653
                     /locus_tag="JQR05_004157"
     CDS             198..653
                     /locus_tag="JQR05_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY5326092.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDVLTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            751..1017
                     /locus_tag="JQR05_004158"
     CDS             751..1017
                     /locus_tag="JQR05_004158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326093.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALASLE
                     AEPVAWKAIFTQIDHEYNTFTALYSDKAEAERWVRLHKVGDFRA"
     gene            1140..1271
                     /locus_tag="JQR05_004159"
                     /pseudo
     CDS             1140..1271
                     /locus_tag="JQR05_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          399 a          275 c          376 g          331 t
ORIGIN      
        1 agggcatacc gtcttaaacc cggcagtact tccggacggg ctgacacagc cgcagtacat
       61 ggatatctgc atggcgatga ttcgttgtgt ggatgcgatt tacatgctga aaggctggca
      121 gcggtcagca ggcgctaagg cagaactggc gctggcggag atactggggc atgcggtgat
      181 ttttcaggag acagccagtg tgcaagacta attaccaggc attacgtgaa cgttattcac
      241 caattcaggt gccggaatgt cctgtttgtg gcgatgaaat gtcgatacag cgcatatttt
      301 ccagaacgca tattgtgtat gcctgcacag gtgaggggga tgatggatat tttaaaactg
      361 gtcgaacttt tgcggatgag cattacctga aatcgcgcgt aaccgtcgtt gatgttagcg
      421 atcctgacgt actggcttta ctgaaagagc tggaagttaa agacaaacgc attgcagaat
      481 tgacagatgt gcttacacaa atgattaatg cgcacaaaac cacaattcgt tttggtcatg
      541 aacgcataac tgaatgtggt ggtgattgcg actcgccgga aaagatgatt tcagaaaatc
      601 cggatatcag aatggcagag gctgttttga gagcaggaat aaaaactgaa taattaaatt
      661 tagcactgta aataaaattt aatccttaac cggagggatt tctgcaccct cagaacatca
      721 ggaggccgcc cgaaatggcg gtaatgaaaa atgactgaat taacaaaaga gcaattaatc
      781 gaagaagcta aattaaaaat agcgattgcg aaatgccatc ccaattcagg cgtggcacga
      841 gtagagggtg agttattcaa aattgcactg gcatcgctgg aagctgagcc ggtggcgtgg
      901 aaggcaatct tcacgcaaat tgaccatgaa tataatacgt tcaccgctct gtattctgac
      961 aaagcagaag cagaacggtg ggtgcgactg cataaagtag gtgattttcg ggcataacac
     1021 cactttatgc agcatcgcca gcgctggtag tgcctgataa gttgccgcgt gaatacataa
     1081 gaggttggcc tcttgcgtat agtgattatg ctgaaggatg gaacgattgt cgtgcagcca
     1141 tgcttcaggg atggcagcca tgccatcaaa ttaccgcctc aatggcaaaa gccattgcgc
     1201 ttaaacttgg tgctgaactg aataacgaag aagcagagat tttcgcggat gggtataacg
     1261 ccgccatgct tcatgccgga aactgaaccg tagcaggagg tgaatcgatg agctggcctg
     1321 aagcattcac aacggtagga attgcaatgg cggtggcgct ggttgtgtat tcgatttgcc
     1381 g
//
LOCUS       DADKXM010000302         1349 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.305, whole genome shotgun sequence.
ACCESSION   DADKXM010000302 DADKXM010000000
VERSION     DADKXM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.305"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..263)
                     /locus_tag="JQR05_004160"
     CDS             complement(<1..263)
                     /locus_tag="JQR05_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326094.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSV"
     gene            288..527
                     /gene="relB"
                     /locus_tag="JQR05_004161"
     CDS             288..527
                     /gene="relB"
                     /locus_tag="JQR05_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY5326095.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            527..814
                     /gene="relE"
                     /locus_tag="JQR05_004162"
     CDS             527..814
                     /gene="relE"
                     /locus_tag="JQR05_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY5326096.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            886..1041
                     /gene="hokD"
                     /locus_tag="JQR05_004163"
     CDS             886..1041
                     /gene="hokD"
                     /locus_tag="JQR05_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY5326097.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          375 a          281 c          349 g          344 t
ORIGIN      
        1 aacactgaca tgctaaagca aaaaatagat gaataagttg agttgtgcat atgtagcctg
       61 accgtcacaa agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaaag
      121 agctgacctg cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca
      181 gtgatcaccg ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg
      241 tagtgcgata cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta
      301 acctgcgtat tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg
      361 taactccttc tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc
      421 cgttcaaaca gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac
      481 ggcttcgtaa tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct
      541 ggattttgac gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca
      601 gttgaaaaag aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg
      661 tggtatgcct gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca
      721 ggttatagac gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga
      781 agtatatagc gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc
      841 accgaaggtg acacttctgc tttgcgttga caggagaagc aggctatgaa gcagcaaaag
      901 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
      961 aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc tgtcttcaca
     1021 gcttacgaac ctgaggagta agagacccgg cgagtgagaa atccctcgcc acctctgatg
     1081 tggcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt gtttttattt
     1141 tcaacgcatt tgaagttctg gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
     1201 cagggataaa agggatggac cccgaacagg ggagtgctac ttatctggaa ggattctgat
     1261 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
     1321 tgaaaatgga aatattgtta gtcattacc
//
LOCUS       DADKXM010000303         1286 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.306, whole genome shotgun sequence.
ACCESSION   DADKXM010000303 DADKXM010000000
VERSION     DADKXM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.306"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..714)
                     /locus_tag="JQR05_004164"
     CDS             complement(<1..714)
                     /locus_tag="JQR05_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5326098.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNGELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAE"
     gene            852..>1286
                     /locus_tag="JQR05_004165"
     CDS             852..>1286
                     /locus_tag="JQR05_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5326099.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          314 a          301 c          311 g          360 t
ORIGIN      
        1 ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca aagtagcgta
       61 acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg cctgacgcag
      121 ttctccgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg cgtattcttc
      181 gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca gagcatcaat
      241 agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga ccgcactaac
      301 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      361 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      421 atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      481 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      541 cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg ggtgagatac
      601 cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      661 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattcagtt
      721 cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat attgatcatt
      781 aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa tagattgtat
      841 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
      901 aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
      961 agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1021 gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct atattgcatg
     1081 acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc gggacaatac
     1141 gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg cgcacattcc
     1201 tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg accgatgtcg
     1261 tttctgaacg ttttgatgca ggcgtc
//
LOCUS       DADKXM010000304         1261 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.307, whole genome shotgun sequence.
ACCESSION   DADKXM010000304 DADKXM010000000
VERSION     DADKXM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.307"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..84
                     /locus_tag="JQR05_004166"
     CDS             <1..84
                     /locus_tag="JQR05_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326100.1"
                     /translation="RDELIDTTQARPIQYCINGTWYNAGSI"
     gene            87..620
                     /locus_tag="JQR05_004167"
     CDS             87..620
                     /locus_tag="JQR05_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5326101.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQMENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(649..1176)
                     /locus_tag="JQR05_004168"
     CDS             complement(649..1176)
                     /locus_tag="JQR05_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5326102.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWAEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEERARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1178..>1261)
                     /locus_tag="JQR05_004169"
     CDS             complement(1178..>1261)
                     /locus_tag="JQR05_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326103.1"
                     /translation="SADNIAGVYYRPIQKYINGTWYNVASV"
BASE COUNT          360 a          283 c          276 g          342 t
ORIGIN      
        1 agagatgaac taattgatac aactcaggca aggccaattc agtattgcat taatgggacg
       61 tggtataacg cggggagtat ttaacgatga tgcacttaaa aaacatttct gctggtaatc
      121 ctgaaacaaa agagcaatac cagctaacga aacaatttaa catcaaatgg ctttatacag
      181 aggatgggaa aaactggtat gaggaacaaa agaactttca gcctgatacg ttgaaaatgg
      241 tctatgacca caacggcgtt attatttgta ttgaaaagga tgtttcagca attaatccgg
      301 aaggcgcaag cgtcgttgag gttcctgata ttacagcaaa tcgccgggct gatatttcag
      361 gtaaatggat gttcaaagat ggcatagtga taaagcgaac ttataccggg gaagagcaga
      421 ggcaacaaat ggaaaatgaa aagcaaagcc tgttgcaact tgtcagggat aaaacccagc
      481 tatgggactc acagctacgg ctgggcatca tttccgccgg gaataagcag aaattaaccg
      541 agtggatgct ctacgcgcag aaggtcgaat ccacagacac ctccagtctg ccagtaacgt
      601 ttcccgaaca accagaatga gagaaggccc gctatcgggc cttaattttt actctggttt
      661 ttgtggccat tctggctttg ccgtatccac acggctgacc agaacactgt agcgttccca
      721 tgactccagt cgtgcgcgtt cctcatctgt tgccatattc agcctgacag agcgctccag
      781 cggctggatg actgattcag cttcggaaag caatgcggct ttttgtgatt cggcctgttg
      841 ctgctgttcg tctgccgtat aaatccgctt aaccacagct ccgtccttaa acatccactt
      901 cccggaatca tcggcacgac gatttgctgt tatatcgggg acctcaacga cgctaaagcc
      961 ttcaggatta agcgtggagg catctttagt gatggcgaca ataatattat tttcatcgta
     1021 aacaatcttt attgtatctg gctgaaagtt cttcacttcc ccataccagt tttttccgtc
     1081 ttctgcccac aaccagatta cgtcaaaatt ctttgttaat tcgtactgtt ccagtgtttt
     1141 agcgttaccc gctttaatgt tctttaagtg catcatatta aacgctcgct acattatacc
     1201 aggtgccatt tatatacttt tgaatcggtc tgtaataaac acccgcaata ttatcggcag
     1261 a
//
LOCUS       DADKXM010000305         1242 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.308, whole genome shotgun sequence.
ACCESSION   DADKXM010000305 DADKXM010000000
VERSION     DADKXM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.308"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(11..202)
                     /locus_tag="JQR05_004170"
     CDS             complement(11..202)
                     /locus_tag="JQR05_004170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326104.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            339..1242
                     /locus_tag="JQR05_004171"
                     /pseudo
     CDS             339..1242
                     /locus_tag="JQR05_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          328 a          309 c          359 g          246 t
ORIGIN      
        1 gtagtgtccg ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg
       61 cggcaaccaa tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt
      121 cggcagcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt
      181 cagcagcgaa gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg
      241 cgacggcggc agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag
      301 cagcaaaacg ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga
      361 aaaaggggat agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga
      421 cagcgaaagc ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc
      481 cggctctgac cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga
      541 tcgccagtac ggcttatgtt atggccgcga ttgctgccat cgtggactcg tcgcctgatg
      601 cactgaacac gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca
      661 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg
      721 ggcttgctac tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg
      781 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg
      841 aatacctcgg tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg
      901 ataccttgtc cggtggactt acttttgaaa acgactcaat ccttgcttgg attcggaata
      961 ctgactgggc gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt
     1021 ggtttgaaac ggcggataac ggtaatgaat atttcaaatg gagacacgcc tcgctggcgg
     1081 ccgggttaaa gacctgatga atctcaaatg ggatacacta aatattctgg ttaatgccgt
     1141 cattaatggt tgccttggca ttggtacgac gaatgcgtta ggtggaagtt caatcgtttt
     1201 aggagataac gataccgggt ttaaacaaaa tggcgatggt ct
//
LOCUS       DADKXM010000306         1241 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.309, whole genome shotgun sequence.
ACCESSION   DADKXM010000306 DADKXM010000000
VERSION     DADKXM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.309"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JQR05_004172"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JQR05_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY5326105.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1019..1198
                     /locus_tag="JQR05_004173"
                     /pseudo
     CDS             1019..1198
                     /locus_tag="JQR05_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          405 a          179 c          247 g          410 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatgttcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgatg ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt atgttgtaat ttatttattg aggttttcta
      841 tggaattctt tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg
      901 agcaaggaaa tgtaatccgg gtaagtatat ttgttttaca tcatatttta gccaagctca
      961 tttattccta ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat
     1021 acgagggagg ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat
     1081 gaagtccttg tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat
     1141 ggtaaaggcg ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttgccgggt
     1201 agtacttcca atcattagaa tatgtgcttt tcaatgaagg a
//
LOCUS       DADKXM010000307         1232 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.310, whole genome shotgun sequence.
ACCESSION   DADKXM010000307 DADKXM010000000
VERSION     DADKXM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.310"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            161..1105
                     /gene="lpxM"
                     /locus_tag="JQR05_004174"
                     /gene_synonym="msbB"
     CDS             161..1105
                     /gene="lpxM"
                     /locus_tag="JQR05_004174"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5326106.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          358 a          235 c          282 g          357 t
ORIGIN      
        1 cgaaaacgat acgctttgtt ggattatatc catgaaactg ctatccgctc tttggatgca
       61 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      121 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      181 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 caaaataggg cgttgggtgg gcagattgag cagaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaatat
      421 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      481 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa
      541 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
      601 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
      661 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
      721 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
      781 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
      841 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
      901 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
      961 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     1021 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     1081 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     1141 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     1201 ctgtgtagat gcagttcgtt aaatttaatt ta
//
LOCUS       DADKXM010000308         1230 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.311, whole genome shotgun sequence.
ACCESSION   DADKXM010000308 DADKXM010000000
VERSION     DADKXM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.311"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..449)
                     /locus_tag="JQR05_004175"
     CDS             complement(<1..449)
                     /locus_tag="JQR05_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5326107.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPL"
     gene            complement(465..1208)
                     /locus_tag="JQR05_004176"
     CDS             complement(465..1208)
                     /locus_tag="JQR05_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY5326108.1"
                     /translation="MISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNY
                     LLIGFNCFELFLLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAA
                     WLLMLPTINIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLG
                     GVITTMIASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLL
                     LGHGGLLDRIDSLIFTAPVFFYFIRYCCY"
BASE COUNT          392 a          316 c          250 g          272 t
ORIGIN      
        1 agcggcacat acaatttaac cttaaaggcc ggagaagcca gcaccagccc acgaattgca
       61 ggtgcataat catgaaccca tgtggcaaca agcactgcgc cgacgctttg cgcgatcact
      121 accacctctt ccagtccgac ctggctgtcg ctggcagcaa aacggacaaa ttcatcgaca
      181 tcctgcactg agcgcataag agacgggcta taaccacgcg gccccgaatt ttttccatgc
      241 ccccgggcat cccaggcata aaaagcagta tcaggcatcg ccagttcatc aacgagatgt
      301 tgtagacgac cagaatgttc atgcccgcga tgaaataaga cgatgacctt tttcgcccct
      361 ggctgtaaag tgggccagtg ccgataatac aacgctgtat tatcactggt ggtaaaaaaa
      421 tgttccccag ggatgcgtga attttccatt ttctctattt tccttcagta acagcagtag
      481 cgaataaaat aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt
      541 ccgccatgtc ctagtaatag ttttccacta tctttaacac caatatctcg tttgatggct
      601 gacatcacta catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc
      661 tgtaatgtat ttagcggtgt cagcaacggt ccaataatca gtgacgcaat catggtggtg
      721 attacgcccc ccagcagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt
      781 cttctgccgc aggatttccc ccataaatac tgtgcaatat cgtttgactc ggttaacgcc
      841 aaaagaaata acaccagtaa cgccccgccc tggatattta tggttggcaa cattaatagc
      901 caggcggcat gactcaaggc aaaaacggtc attatccatc cccaaaaaat ggcgctcacg
      961 gtatgctgaa aaccggaggg gtcacccaca aaaactcgcc aggtggctaa tatcagaaac
     1021 ccagtcaaag gtataaataa cagaaacagc tcaaagcaat taaaaccgat aagcaaatag
     1081 ttaagaggaa taacccaata taaccaacgc gaaaaatgta cggatataag cgtacaatat
     1141 tctttcaatg ccagaaaact tatcagcgta aaaaacgtca acgtcatcca gcgtgggcct
     1201 gaaatcacca gtgaaaaaca aatgacgatg
//
LOCUS       DADKXM010000309         1199 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.312, whole genome shotgun sequence.
ACCESSION   DADKXM010000309 DADKXM010000000
VERSION     DADKXM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.312"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JQR05_004177"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JQR05_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY5326109.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          214 c          259 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaataatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DADKXM010000310         1185 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.313, whole genome shotgun sequence.
ACCESSION   DADKXM010000310 DADKXM010000000
VERSION     DADKXM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.313"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..331
                     /locus_tag="JQR05_004178"
     CDS             <1..331
                     /locus_tag="JQR05_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY5326110.1"
                     /translation="ISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMV
                     DDYNSLR"
     gene            complement(381..>1185)
                     /locus_tag="JQR05_004179"
     CDS             complement(381..>1185)
                     /locus_tag="JQR05_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY5326111.1"
                     /translation="ITLLREQLDIPLFDSDLIGLYFACALERHQNERQPIILLSDQNA
                     IATINQLAIERDVLHCRVIIARSLSELVAIREEIDPLLIINNSHYLLDDAVNNYITVK
                     NIITAAGIEQIKHFLATAFIRQQPERFFSAPGSFHYSNVRGESWQHITRQICAQLVAQ
                     HHITADEAQRIIAREGEGENLIVNRLAIPHCWSEQERRFRGFFITLAQPVEVNNEVIN
                     HVLIACAAADARHELKIFSYLASILCQHPAEVIAGLTGYEAFMELLHKG"
BASE COUNT          337 a          261 c          292 g          295 t
ORIGIN      
        1 gatttcagcg aaacaaatta ataacctgat atcgcaagag aagtttgatg ttgatgcggc
       61 aatgaagaaa gtgtctgaac tggaaacgct ggtggcgcag gccaaagaag cggataaagg
      121 cggcatgaat ttctcgttta ttaattcggc aggccagtat caactcgagg ctaaaaaata
      181 cgttcgccgc atcagagata aagtcccgta ctctgactgg gataaagagc aacttcagga
      241 tgcaaactca agctggatgg tcgaagactc tttcccgaga gcattacgcg agtacaacga
      301 aatggttgat gactataata gcctgcgtta aacgtttttg atgataaatg cagtgtcgga
      361 tgcggcgcat ccgacacagt tcaccccttg tgaagtaact ccataaatgc ctcatatcct
      421 gttaacccgg caataacctc cgccggatgc tgacacaata tgcttgccag atagctaaat
      481 atttttagct catgacgcgc atcggcggcg gcgcaggcga tcaagacatg gttaatgact
      541 tcgttattca cctcaactgg ttgggcgagg gtaataaaaa atccacgaaa acgtcgctcc
      601 tgttcgctcc agcaatgtgg gatggcgagg cgattaacaa tcaggttttc accttcgcct
      661 tcgcgggcga tgatccgttg cgcttcatcg gcggtaatat ggtgttgtgc gactaattgc
      721 gcacaaattt gccgggtaat atgttgccag ctttcaccgc gtacattcga ataatgaaaa
      781 cttccggggg cagagaaaaa acgctccggc tgttggcgaa taaatgccgt cgccagaaaa
      841 tgttttattt gttcgatacc ggcagccgta atgatatttt ttacggtgat gtaattattt
      901 accgcgtcat ccagtaaata atggctgttg ttaatgatca ataacggatc aatctcttcg
      961 cgaatggcaa caagttcgct taagctacgg gcaataatca cccgacaatg taaaacatcg
     1021 cgctcaatgg cgagctgatt aatagtggca atcgcgttct ggtccgagag caaaatgatc
     1081 ggctggcgtt cgttttgatg ccgttccagc gcacaggcaa aatacaaacc gatcagatcg
     1141 ctgtcgaaaa ggggaatatc gagctgttca cgcagtagcg taata
//
LOCUS       DADKXM010000311         1164 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.314, whole genome shotgun sequence.
ACCESSION   DADKXM010000311 DADKXM010000000
VERSION     DADKXM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.314"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            211..>1164
                     /locus_tag="JQR05_004180"
     CDS             211..>1164
                     /locus_tag="JQR05_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5326112.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT          245 a          362 c          309 g          248 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 ctcatcacgc tgcgtaagca aagagacgag gtggcgcttg aggtagaaca gcgagttctt
     1021 gctcaccctc tttacccggt cctgaccagt atgcccggag tcggtgtcag gaccgcagcc
     1081 agactcctca ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat
     1141 gctggccttg cgccggtaac tcgg
//
LOCUS       DADKXM010000312         1139 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.315, whole genome shotgun sequence.
ACCESSION   DADKXM010000312 DADKXM010000000
VERSION     DADKXM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.315"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..186
                     /locus_tag="JQR05_004181"
     CDS             <1..186
                     /locus_tag="JQR05_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY5326113.1"
                     /translation="KQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHAD
                     ELYRSCLARFGDDGENL"
     gene            465..777
                     /locus_tag="JQR05_004182"
                     /pseudo
     CDS             465..777
                     /locus_tag="JQR05_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            802..963
                     /locus_tag="JQR05_004183"
     CDS             802..963
                     /locus_tag="JQR05_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY5326114.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHYKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT          271 a          302 c          308 g          258 t
ORIGIN      
        1 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg
       61 ctggagaagc tcaacgaact ggacgcggat gaacaggctg atatctgtga atcgcttcac
      121 gaccacgccg atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac
      181 ctctgacata tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc
      241 agacaagccc gtcagggcgc gtcagcaggt tttagcgggt gtcagggcgc agccatgacc
      301 cagtcacgta gcgatagcgg agtgtatact ggcttaatat gttacagcgg agtaacactt
      361 aatgaaagtg cttcatgtga caggggaaaa tgcagctgca gggcgtacag gagaaaatgg
      421 aggttacggt gatatgacgc ctcctcgctc actcggtcgc tgccgctcct gccgttcgac
      481 tgcggcgggc gttacctgct cgcggataag tgcaaaggca tacggcatga caagcggggc
      541 agaaaaagga tgcggcgctg ccgtttttcc ataggctccg cccccctgac aagcatcaca
      601 gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      661 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      721 ttccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggtaggca
      781 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgacta ccacgcccgt
      841 tccggtaact atcaacttga gtccaacccg gaaagacact acaaatcgcc agtggcggta
      901 gccattggta acggatttag aggagttagt cttgaagtta tgcgcctgtt aaggctaaac
      961 tgaaaggaca aatttggtta ctgcgctcct cataagccag ttaccttggt tcaaagagtt
     1021 ggttgctctg agaaccttcg agaaaccgcc ttgcagggcg gttttttcgt ttctgggaaa
     1081 gagactacga cgacaagcaa atgatctcaa gtagatctgt tgttaattgt cctgttcca
//
LOCUS       DADKXM010000313         1099 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.316, whole genome shotgun sequence.
ACCESSION   DADKXM010000313 DADKXM010000000
VERSION     DADKXM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.316"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..557
                     /locus_tag="JQR05_004184"
     CDS             <1..557
                     /locus_tag="JQR05_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY5326115.1"
                     /translation="MNQGNRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSG
                     QQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIA
                     PVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNI
                     KSNNANNWYLTIINDHGNYISDKI"
     gene            573..>1099
                     /locus_tag="JQR05_004185"
     CDS             573..>1099
                     /locus_tag="JQR05_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5326116.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKST"
BASE COUNT          353 a          196 c          255 g          295 t
ORIGIN      
        1 tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg gattgatgat ggcgatacgt
       61 cgttaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg gcaaaaatag
      121 gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg cccactaata
      181 aagaaagcat tttttatctg aatgttctgg atattccacc aaatagtcca gagcaagaag
      241 gcaagaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac cggccagcgg
      301 gtattgctcc ggtaaataaa gcgaccttta aaaagttact ggtaaatcgc agtggcaatg
      361 gattggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc aaagctaata
      421 atgttaaagt caattatgaa actattatga ttgccccctt agaaagtcag agtgttaata
      481 tcaaaagtaa taatgcaaat aactggtatc tgaccattat caatgaccac ggcaactata
      541 ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca cttgcatcag
      601 caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc tttgataccc
      661 attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt gatgataatc
      721 aacccttacc ggggcagtat gacatcgata tttatgtcaa taagcaatgg cgcgggaaat
      781 atgagattat tgttaaagac aacccgcaag aaacatgttt atcaagagaa gttatcaagc
      841 ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca tttgagcaac
      901 ttgttcaggg tgggagctat agctgggata tcggggtttt tcgtctcgat ttcagtgtcc
      961 cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc accggaaaac tgggagcggg
     1021 gtattaatgc gttttatacc tcttattatg tgagtcagta ttacagcgac tataaagcgt
     1081 cgggtaacaa caagagtac
//
LOCUS       DADKXM010000314         1085 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.317, whole genome shotgun sequence.
ACCESSION   DADKXM010000314 DADKXM010000000
VERSION     DADKXM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.317"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>1085
                     /locus_tag="JQR05_004186"
     CDS             <1..>1085
                     /locus_tag="JQR05_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326117.1"
                     /translation="LSDGKNENGQLFDKQKALKRIKELEGEQIKTARREMVLAVVGTM
                     KAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQ
                     EKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGEEEIFTGLASLNDLVRLA
                     AAMGSEFPFDEYAEVQKLPVIDVEFSHLVGMDECQGTLTLVDTPGPNEAGQPQMEVMM
                     RDQLQKASAVLAVMDYTQMNSKADEEVRKELNAIADVSVGRLFVLVNKFDEKDRNGDG
                     ADAVCQKVPAMLNSDVLPASRVYPGSSRQAYLANRALHELRKNGTLPVDEAWVDDFVR
                     EAFGRMKKDYVCKDSELATEGATDLWE"
BASE COUNT          294 a          264 c          307 g          220 t
ORIGIN      
        1 gttatctgac ggtaagaacg aaaacgggca gctttttgat aaacagaaag cactgaaaag
       61 aattaaggag ctggagggcg aacaaatcaa aaccgcccgc agggagatgg tgctggctgt
      121 tgtcggcacg atgaaagcag gcaaatcaac caccatcaac gccattgtgg ggcaggaaat
      181 tctgcctaac cgtaaccgcc ccatgacctc tgtaccgacg ctcatccgcc acgttcccgg
      241 aaaaactgag ccggttctcc atctggaaca tattcagcct gtccgcaatt tattaatcac
      301 actgcaggaa aaactcgcca ccccggcagg acagcaggtc gcacagaccc tgcagcaaac
      361 cggggatacc cgcgaactgc tggatattct gacggatgat ggctggctca aaaatgaata
      421 ccacggggag gaggaaatct ttaccggact ggcatcgtta aacgatctgg ttcgtcttgc
      481 tgcggcaatg gggagtgaat ttccttttga tgaatacgca gaagtgcaga aactgccggt
      541 gatcgacgtg gaattcagcc atctggtggg gatggatgaa tgccagggaa cactcacact
      601 ggtggatacc cccggcccta atgaagccgg acaaccgcag atggaagtga tgatgcggga
      661 tcaactgcag aaagcctctg cggtgctggc ggtgatggac tacacccaga tgaactcgaa
      721 agccgatgag gaggttcgta aagagcttaa tgccattgct gatgtgtcag tcggccgcct
      781 gtttgtgctg gtcaataaat ttgatgaaaa agaccgtaat ggcgatgggg ctgatgcagt
      841 ctgtcagaag gtaccggcaa tgctgaacag cgatgtgctg cccgcctccc gcgtttatcc
      901 cggatcctca cgccaggcat acctggctaa ccgtgcgctt catgagttac ggaaaaacgg
      961 aacccttcct gttgatgaag cctgggtcga tgattttgtc agggaagcct tcggtcgcat
     1021 gaaaaaagat tacgtctgta aagacagcga actggcaact gaaggggcga cagacctgtg
     1081 ggaag
//
LOCUS       DADKXM010000315         1071 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.318, whole genome shotgun sequence.
ACCESSION   DADKXM010000315 DADKXM010000000
VERSION     DADKXM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.318"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..215
                     /locus_tag="JQR05_004187"
     CDS             <1..215
                     /locus_tag="JQR05_004187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5326118.1"
                     /translation="YKNEGTAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLK
                     ARAITVNGNASQGTIEALINVIYTWQ"
     gene            274..>1071
                     /locus_tag="JQR05_004188"
     CDS             274..>1071
                     /locus_tag="JQR05_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5326119.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG"
BASE COUNT          310 a          221 c          251 g          289 t
ORIGIN      
        1 attacaaaaa tgaaggtact gccgaaaata ttcagataga gctgagggat gaccaggatg
       61 ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga tgagatcact cgtaatgcac
      121 agtttccact taaggcaaga gctatcacgg tgaatggaaa cgcaagccag ggaacgatcg
      181 aggcgctaat caatgtgatc tacacctggc aataacgtcg attcgatttt cttagccttc
      241 tttttaaatc aatttttaac cttaggatat atcatgggta aaacaatcag tataaaggtc
      301 ctgttcggta tttatcttct attaatggca ggcaaagttt ttgccttcag ttgtaatgtc
      361 gatggcgggt caagtattgg agcaggtaca acctctgttt atgttaatct cgaccctgta
      421 atacagccgg gccagaatct ggttgtagac ttgtctcagc atataagttg ctggaatgat
      481 tacggcggct ggtacgacac tgatcatata aacctggtac aaggctcagc tttcgcaggc
      541 tccctacagt catataaagg atcgctgtac tggaataatg tgacctaccc cttcccgttg
      601 acgacaaata ccaacgtatt ggatattggt gataagacac cgatgccatt accgttaaaa
      661 ctctatatta ccccagttgg ggcggccggt ggagttgtca taaaagcggg ggaagtcatt
      721 gcccgtattc atatgtataa aattgcaaca ttaggaagcg gaaatccgcg taattttacg
      781 tggaatatta tttcaaataa cagtgttgtt atgccaacag gcggctgcac cgttgatagt
      841 cgtaatgtca ccgtggatct cccggacttt cctggaagcg cagaaatacc ccttggcgta
      901 tattgctcaa gcgaacaaaa gctctcattt tatctttcgg gtgccaccac tgacagtagc
      961 cgacaagtgt ttgctaacac agcacctgat gcaacaaaag catcgggagt gggtgtcact
     1021 ctaatgcgta atggaaaaat actggctacg ggagaaaatg tctccctggg g
//
LOCUS       DADKXM010000316         1061 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.319, whole genome shotgun sequence.
ACCESSION   DADKXM010000316 DADKXM010000000
VERSION     DADKXM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.319"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(451..736)
                     /locus_tag="JQR05_004189"
                     /pseudo
     CDS             complement(451..736)
                     /locus_tag="JQR05_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(885..>1061)
                     /locus_tag="JQR05_004190"
     CDS             complement(885..>1061)
                     /locus_tag="JQR05_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5326120.1"
                     /translation="QTERLLTWRGMYQQLLKVDPLKCELCGGQMRFTGLKMGYRLAEQ
                     IMMHEPLARMRWCG"
BASE COUNT          335 a          235 c          194 g          297 t
ORIGIN      
        1 ttcccgtatt ggaaattaat aaagcagaga caaacacaat atatttaata ctcactaatt
       61 tagagttaat aactcacacc ccatttttat gaaaattaca taaaaataat ttagaaaaaa
      121 aataaacaaa tatccacccg ttaaaaacaa aaaaaccaaa tagtgccaaa acatgaacca
      181 tttttactat ttatatttgc gttgatttgg cgtttacctg cgacgcaatt atataacaat
      241 tgattaatgc tttggttaat taacatttac atacattaat aatgtaacgc tattgaggta
      301 taatatataa tgcgggagtt gacatgggat cccgtatatc agcagttatt tttactcggc
      361 agttcattga ttattatcaa taataaacgt aaaccacgtg gccgaaatat tgatgggata
      421 acaccaagag gaaaagggtg atgactccag gaacatatgt ttttcgataa aagcaccgat
      481 aactttttca tgaaatttta cagagcgaga gaaacagatg gttctgcgag ccaggcgttt
      541 gattcgggtt ctcaggggca gattgttgcg ctcaatgcgc tgagtaaaaa tcttaccagt
      601 cagatacttc tctttcggta actctctggt gtaactaccc cattcgtcag tggtcaacat
      661 accgatggta aaagggggaa gcagcgccag cagttcatcg acaggaactt aaatccccaa
      721 aagattagaa aagcaccgaa gaatgcgcgt ttattcaaca cgacctgatc atcacgtaat
      781 tggtatagac acttgatatt gcttacattg attgattcaa tatatctctg atggtttttt
      841 gctccgttcc cctcaacatg gacgcaactt cccctctgcg gcgctcagcc gcaccaccgc
      901 attcgtgcca gcggctcatg catcataatc tgctctgcca gacggtaacc catcttcagt
      961 cccgtaaacc gcatctgacc tccgcacagc tcgcacttca gcgggtcaac cttcagtaac
     1021 tgctgataca tccctctcca tgtgagtaag cgctccgtct g
//
LOCUS       DADKXM010000317         1054 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.320, whole genome shotgun sequence.
ACCESSION   DADKXM010000317 DADKXM010000000
VERSION     DADKXM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.320"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..743
                     /locus_tag="JQR05_004191"
     rRNA            <1..743
                     /locus_tag="JQR05_004191"
                     /product="16S ribosomal RNA"
     gene            800..876
                     /locus_tag="JQR05_004192"
     tRNA            800..876
                     /locus_tag="JQR05_004192"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Ile,seq:gat)
     gene            919..994
                     /locus_tag="JQR05_004193"
     tRNA            919..994
                     /locus_tag="JQR05_004193"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:952..954,aa:Ala,seq:tgc)
BASE COUNT          265 a          244 c          305 g          240 t
ORIGIN      
        1 gtaaacgatg tcgacttgga ggttgtgccc ttgaggcgtg gcttccggag ctaacgcgtt
       61 aagtcgaccg cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc
      121 cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctggtct
      181 tgacatccac ggaagttttc agagatgaga atgtgccttc gggaaccgtg agacaggtgc
      241 tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa
      301 cccttatcct ttgttgccag cggtccggcc gggaactcaa aggagactgc cagtgataaa
      361 ctggaggaag gtggggatga cgtcaagtca tcatggccct tacgaccagg gctacacacg
      421 tgctacaatg gcgcatacaa agagaagcga cctcgcgaga gcaagcggac ctcataaagt
      481 gcgtcgtagt ccggattgga gtctgcaact cgactccatg aagtcggaat cgctagtaat
      541 cgtggatcag aatgccacgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac
      601 catgggagtg ggttgcaaaa gaagtaggta gcttaacctt cgggagggcg cttaccactt
      661 tgtgattcat gactggggtg aagtcgtaac aaggtaaccg taggggaacc tgcggttgga
      721 tcacctcctt accttaaaga agcgttcttt gtagtgctca cacagattgt ctgatgaaaa
      781 tgagcagtaa aacctctaca ggcttgtagc tcaggtggtt agagcgcacc cctgataagg
      841 gtgaggtcgg tggttcaagt ccactcaggc ctaccaaatt tgcacggcaa atttgaagag
      901 gttttaacta catgttatgg ggctatagct cagctgggag agcgcctgct ttgcacgcag
      961 gaggtctgcg gttcgatccc gcatagctcc accatctctg tagtgattaa ataaaaaata
     1021 cttcagagtg tacctgcaaa ggttcactgc gaag
//
LOCUS       DADKXM010000318         1046 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.321, whole genome shotgun sequence.
ACCESSION   DADKXM010000318 DADKXM010000000
VERSION     DADKXM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.321"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            241..435
                     /gene="bfd"
                     /locus_tag="JQR05_004194"
     CDS             241..435
                     /gene="bfd"
                     /locus_tag="JQR05_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY5326121.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            508..984
                     /gene="bfr"
                     /locus_tag="JQR05_004195"
     CDS             508..984
                     /gene="bfr"
                     /locus_tag="JQR05_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY5326122.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(981..>1046)
                     /locus_tag="JQR05_004196"
     CDS             complement(981..>1046)
                     /locus_tag="JQR05_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY5326123.1"
                     /translation="FGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          304 a          217 c          245 g          280 t
ORIGIN      
        1 aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt
       61 acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat
      121 ttgagctcga aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt
      181 gctattgtaa tcataaccat tctcatttac actttgcgca gaatttgaac gggagcaatc
      241 atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag
      301 tttcatccgc aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt
      361 aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt
      421 aaagagattg cctgaagccc acgcttattt tttgacatcc cgctagcccc atctacgctc
      481 taatagtgga agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc
      541 aataaactat tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg
      601 tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag
      661 atgaaacacg ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta
      721 caggatttag gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg
      781 cgattagaac tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt
      841 catgactatg ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc
      901 gactggctcg aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa
      961 tcacaaatta aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa
     1021 cccgcagccg ccagaaatgg cccaaa
//
LOCUS       DADKXM010000319         1041 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.322, whole genome shotgun sequence.
ACCESSION   DADKXM010000319 DADKXM010000000
VERSION     DADKXM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.322"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            92..274
                     /locus_tag="JQR05_004197"
     CDS             92..274
                     /locus_tag="JQR05_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY5326124.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     RVTELFALLHTEEGCC"
     gene            complement(375..704)
                     /locus_tag="JQR05_004198"
     CDS             complement(375..704)
                     /locus_tag="JQR05_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY5326125.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          268 a          238 c          239 g          296 t
ORIGIN      
        1 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
       61 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
      121 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
      181 tggagcgcac aacgtgttag taaccagtgc agttaataac aatagagtta cagaactttt
      241 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
      301 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
      361 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
      421 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
      481 cgatcttcat agtcaccttt catgctggcg atgatgccct ggattgcttc aacgctcatc
      541 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
      601 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
      661 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
      721 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
      781 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
      841 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
      901 agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
      961 attggtgttt ccacgacttt caggtcatgg tggttatcga gcaactgacg cagctcttcc
     1021 acagcatcgt tcaatttttc c
//
LOCUS       DADKXM010000320         1038 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.323, whole genome shotgun sequence.
ACCESSION   DADKXM010000320 DADKXM010000000
VERSION     DADKXM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.323"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..261
                     /locus_tag="JQR05_004199"
     CDS             <1..261
                     /locus_tag="JQR05_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326126.1"
                     /translation="DAEVLRFDSFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG
                     YPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVAPPF"
     gene            261..>1038
                     /locus_tag="JQR05_004200"
     CDS             261..>1038
                     /locus_tag="JQR05_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326127.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPLVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPV"
BASE COUNT          224 a          265 c          309 g          240 t
ORIGIN      
        1 gacgcagaag ttctgcgctt tgatagtttt gacgatgccg ttgaatgctg cgcagacctg
       61 aatattccgt tctttgtgga tgcgggaaac aaaaagctgg tcttctggtt tgtacgtgtt
      121 gatgacgaag ggtatccgga aatcgcccgc tgcacggagc gggaatttgc gaccattctt
      181 gccggtatca gcgccggcgg tatgtactgc ccggagtgtg gcacggttca ctggccggac
      241 ggagtcgccc cgcccttctg atgcttcccc gttttgccga tatttttcag cagggaaacc
      301 gctggcttaa ctggctggag aaacaaccgg aaggggcagt ccgtccgttg gtaattgaat
      361 cagtgacaaa aatcatggcc tgcgggacca cactgatggg ctacacgcag tggtgttgtt
      421 catcaccgga ctgttgccat acaaaaaagg tctgcttccg gtgtaaaagc cgctcctgcc
      481 cgcactgcgg ggtgaaggct ggcgcacagt ggatacagta tctgctgagc ctggtccccg
      541 actgcccgtg gcagcatatt gtgttcacac ttccctgcca gtactggtcc ctggtgttcc
      601 acaaccggtg gttactggca gagatgagcc gcattgcagc ggatgtgata ctggaaatct
      661 gccatcagac agatgtggag ccggggatat tcacggtgat ccacacatgg gggcgtgacc
      721 agcagtggca tccgcatatc catttatcga caactgccgg tggtgtgacg tcgggccaca
      781 cctggaaaaa tcttcatttt tacgcccgta aggtgatgag catgtggcgc taccggataa
      841 cacggttgct gtcacggaaa taccctgacc tggtgatgcc ggatgcgctg gcagcagaag
      901 gaagcagtaa acgggaatgg aatcgcttcc tggacacgca ttaccgccgt ggctggaatg
      961 tcaacgtatc ccgggtgatg gataatgcca cccatgtggc ggtgtacttt ggctcttacc
     1021 tgaaaaagcc accggtgc
//
LOCUS       DADKXM010000321         1023 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.324, whole genome shotgun sequence.
ACCESSION   DADKXM010000321 DADKXM010000000
VERSION     DADKXM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.324"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            49..372
                     /locus_tag="JQR05_004201"
     CDS             49..372
                     /locus_tag="JQR05_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY5326128.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            362..>1023
                     /locus_tag="JQR05_004202"
     CDS             362..>1023
                     /locus_tag="JQR05_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY5326129.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQAIAEGVTRGIDALYHAGEIKGRQDVIQALTEAGIDVVRVTRSSISIADPN
                     GG"
BASE COUNT          242 a          242 c          351 g          188 t
ORIGIN      
        1 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gcgagaggat gctcacgata
       61 cgggttactg atgatgaaca tgcccggtta ctggaacgct gtgagggtaa acagctggcg
      121 gtatggatgc ggcgggtctg cctgggtgag ccggttgccc gttccgggaa actgccgaca
      181 ctggcaccgc cgttactgcg tcagctggct gccatcggga ataacctgaa tcagacagcg
      241 aggaaggtga acagcgggca gtggtcttcc ggtaaccggg ttcaggtggt ggccgcactg
      301 atggccatcg gggacgagct gcgccggctg cgtctggccg tcagggaaca ggggacgcgg
      361 gatgatagtt aagtttcatg cccggggaaa aggtggtggc agtggtccgg ttgattactt
      421 gctgggaagg gagcgtaacc gggaaggggc aacggtgctt cagggtaatc cggaagaagt
      481 acgggaactc atcgatgcca cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt
      541 cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt
      601 actgatgccc ggtctcgaaa aaaatcagta cagcatcctg tgggtggagc accaggacaa
      661 gggacggctt gagctgaatt ttgtcattcc gaacatggag ctacagaccg gaaaacgcct
      721 ccagccgtac tacgaccgcg cagacaggcc tagaattgat gcctggcaga cgctggttaa
      781 tcaccattac gggctgcatg acccgaacgc cccggagaac cgcaggacgc tgacactccc
      841 tgataatctg ccggaaacga aacaggcgat tgctgagggc gttacacgcg ggatagacgc
      901 gctttaccat gccggagaga taaaaggccg tcaggatgtg attcaggcgc tcacagaggc
      961 agggattgac gtggtcaggg tgacgcgaag cagtatcagc attgcagacc cgaacggcgg
     1021 gaa
//
LOCUS       DADKXM010000322         1013 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.325, whole genome shotgun sequence.
ACCESSION   DADKXM010000322 DADKXM010000000
VERSION     DADKXM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.325"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQR05_004203"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQR05_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY5326130.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          293 a          164 c          244 g          312 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aac
//
LOCUS       DADKXM010000323         1013 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.326, whole genome shotgun sequence.
ACCESSION   DADKXM010000323 DADKXM010000000
VERSION     DADKXM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.326"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..997)
                     /locus_tag="JQR05_004204"
                     /pseudo
     CDS             complement(1..997)
                     /locus_tag="JQR05_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          302 a          264 c          219 g          228 t
ORIGIN      
        1 tcagggtcag aaaatagcca ttaacgggcg taccagaagc gttggtacga cataaactgg
       61 ctggaatata tttgctgtca gcatcaccta atagcacttt aagcggggca tcaatcgaga
      121 ataccagcgc agctacttgt cccagggtat tcgcaatggc gtaaatcacc attaaagtat
      181 tacccatgtt gtaatactcg cccagcttct gaaaggcgta atattgaccg ttcgtcatta
      241 agtcatccgg gatattacgc gagtcaaaca tcatccccat cgccagtgag cccagaatgg
      301 cacaaaccgc aaccatcacc gccaggcata gcatcccttt tggaaattct tttcctgggt
      361 tgcgcgtttg attaacgtaa ggagaaatct tctctgctcc gccaaccgcg aaaaccagca
      421 ttgaaatagt ggtaatgtag gtaaagtcga tatgaggaat gaacgtttcc caggtaatgt
      481 ttgtggtcgc aatatgcact tcagtaatcg caggcgcggt taccgccatc gccacataca
      541 ggagcgacat aacaaacatc gcaatccctg ccacagaacc gacgattttc agcgatttca
      601 taccgcgtga agcaacccac ataaagaaga taaacagcac cagcgttaac ccctgtaacg
      661 ctacgactga atattctttg attagcgaac cgtcgccttt catcgcccaa ccgagcgcaa
      721 tcagaattgc ctagggtttt tgtgccagat agggaatatg caccacccag taggtccacg
      781 cggcgagata agccagtccg ggtcccatcg tgtgtttaat ccaggtactg accccgccct
      841 tcccatcttt gaaggtcgag cctaactgac caacaattag cgcataaggt gtgaaataga
      901 gtgcaaagat aaacacccat gaaaaaacaa ccaccagccc ctggttggca tagttattaa
      961 caacgttgcc aaaaccccag acagtgataa aagacatcag cgcaatgttg tac
//
LOCUS       DADKXM010000324          996 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.327, whole genome shotgun sequence.
ACCESSION   DADKXM010000324 DADKXM010000000
VERSION     DADKXM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.327"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..534
                     /locus_tag="JQR05_004205"
                     /pseudo
     CDS             1..534
                     /locus_tag="JQR05_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          303 a          222 c          210 g          261 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 gaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcatta
//
LOCUS       DADKXM010000325          989 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.328, whole genome shotgun sequence.
ACCESSION   DADKXM010000325 DADKXM010000000
VERSION     DADKXM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.328"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(21..161)
                     /locus_tag="JQR05_004206"
     CDS             complement(21..161)
                     /locus_tag="JQR05_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326131.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            300..971
                     /gene="queE"
                     /locus_tag="JQR05_004207"
     CDS             300..971
                     /gene="queE"
                     /locus_tag="JQR05_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY5326132.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          282 a          203 c          231 g          273 t
ORIGIN      
        1 agcgcattat aaatttctca ttaaatctgg ataaataaat tgacggcggc atcggtaatt
       61 ttagattcgt tatcattaaa aactaataca gaaaaaataa atataatttt ggtgaatgtt
      121 ttgacatgat taaatccatt ttctttattt tcttctgaca taatacgtta ctccctttaa
      181 ttattaacaa tatgtttcgt atttttcttt aaatctgcat gtaacagaat gttaataaga
      241 catgattgtt gcggcaggat ctgcaataat taacaattat ccacttcaca gagaatgcta
      301 tgcagtaccc gattaacgag atgttccaga ccctgcaagg tgagggttac tttaccggcg
      361 tccccgccat ttttattcgt ttacagggat gcccggttgg ctgtgcctgg tgcgacacca
      421 aacacacctg ggaaaagctt gaggatcggg aagtctccct tttcagcatt ctggcgaaga
      481 ccaaagagag tgataagtgg ggggcggcga gcagtgaaga cttgctggct gtcattggtc
      541 gtcagggata taccgcgcgg catgtggtga ttacgggtgg tgagccttgc attcatgatt
      601 tgctgccact gactgacctg ctcgaaaaga acggttttag ctgccagatc gaaaccagtg
      661 gaactcatga agtacgctgc acaccgaata cctgggttac cgtatcgcca aagctgaaca
      721 tgcgcggcgg ctatgaagtg ttgtcacagg cactggagcg agccaacgaa atcaagcatc
      781 cagtggggcg cgtacgcgat attgaagcac tggatgaact actggcaacg ctgaccgatg
      841 ataaaccgcg agtcattgca ctgcagccga ttagccaaaa ggatgatgcc acacgattgt
      901 gcattgaaac ctgtattgcg cgtaattggc gtttgtcgat gcaaacacat aaatatttaa
      961 atattgcctg attacacatt tataagcgt
//
LOCUS       DADKXM010000326          983 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.329, whole genome shotgun sequence.
ACCESSION   DADKXM010000326 DADKXM010000000
VERSION     DADKXM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.329"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQR05_004208"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQR05_004208"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY5326133.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          258 a          219 c          223 g          283 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaactaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccgtaagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgaaaaa aaacctcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADKXM010000327          979 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.330, whole genome shotgun sequence.
ACCESSION   DADKXM010000327 DADKXM010000000
VERSION     DADKXM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.330"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JQR05_004209"
     CDS             complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JQR05_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAY5326134.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(562..>979)
                     /locus_tag="JQR05_004210"
     CDS             complement(562..>979)
                     /locus_tag="JQR05_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5326135.1"
                     /translation="NCELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVK
                     CTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDA
                     AAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          258 a          266 c          220 g          235 t
ORIGIN      
        1 atcgactacg ccaaattata cgaattaata taatattaat tataattgat cataaatatc
       61 gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata attggcaaaa
      121 aagtttagtg tgacattgcc gtttgcatct acttcctcag tcgggctcgc ctcccccagt
      181 gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgcgc cattgcggga
      241 tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa aatcgcaact
      301 gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt ggtatcgcca
      361 gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc aatatccgct
      421 gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt aatcgttcct
      481 aaagggtcag ctgcccatcc actggaactc cacagtaacc cgcagaaaca gctaaagaat
      541 actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca gcgcgctaaa
      601 cggcccttcg gtcggttttt gaccggtaat actgataggc caggcgcgaa gtgtgacatt
      661 ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt taggcgtgat
      721 cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg acaccatcgc
      781 ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg cagcaacatt
      841 cgtacatttg accgcaatgc tcttggtttg cggcatgacg ccagctggtc gattccccgg
      901 ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa caatctgccc
      961 ggcatttaat tcgcagttt
//
LOCUS       DADKXM010000328          962 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.331, whole genome shotgun sequence.
ACCESSION   DADKXM010000328 DADKXM010000000
VERSION     DADKXM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.331"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..483)
                     /locus_tag="JQR05_004211"
     CDS             complement(1..483)
                     /locus_tag="JQR05_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5326136.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="JQR05_004212"
     CDS             complement(474..776)
                     /locus_tag="JQR05_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5326137.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          293 a          235 c          194 g          240 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctgggta caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aa
//
LOCUS       DADKXM010000329          949 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.332, whole genome shotgun sequence.
ACCESSION   DADKXM010000329 DADKXM010000000
VERSION     DADKXM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.332"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..586
                     /locus_tag="JQR05_004213"
     CDS             <1..586
                     /locus_tag="JQR05_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAY5326138.1"
                     /translation="LQDKTIHVFGPPGTRAMTAASWQVFDRDITLRMEEEGKPDPRKL
                     VTAQDVGEGVVYKDDTVTVTALKVPHSPFPDGEAFAYRFDTQGKRIVFSGDTSYFPPL
                     ATFSEGADILVHEAVHEPSVAKLADSIGNGKTLAKAIASHHTTIEDVGKIANEAKVKK
                     LILSHLVPITVPDETWKQEAQKTIQALSSLVMKI"
BASE COUNT          277 a          239 c          202 g          231 t
ORIGIN      
        1 tctgcaggac aaaactattc atgtattcgg tccgccaggc acccgagcca tgaccgctgc
       61 cagttggcag gtcttcgatc gggacatcac actgcgtatg gaagaagaag gaaaacccga
      121 tcctcgcaaa ctcgttacag ctcaagatgt tggtgaaggc gtagtttata aagatgatac
      181 cgtgaccgtg actgcattaa aggtccctca ttctcctttc cctgacggtg aggcttttgc
      241 ttatcgcttc gacacacaag gcaagcgaat tgtcttctca ggagatacct cgtactttcc
      301 cccactggca actttctcag aaggcgctga tatacttgtg catgaggccg tacatgagcc
      361 ctccgtcgct aagctggcag acagtattgg caatggtaaa acactggcaa aagccattgc
      421 ttctcaccat acgactattg aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa
      481 actcatcctt agccacctgg taccaatcac cgttccggat gaaacatgga agcaagaagc
      541 acagaaaact attcaggccc tatcgtcgtt ggtcatgaaa atatgacgat taccattcca
      601 ttcgccccat aaaaaattcc gtccccgtta gcccttgtta gcggggaata ttatgatttg
      661 ccttactgta agttgatcac gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa
      721 gtaccaacgt tcgcctgaaa cgtctcttcc cgacaaccgc gttcaatgct tcaattaaat
      781 aacaaaaaca acacacaaaa ttaacaatcg tattccgatt aatactgtag aggtcgacat
      841 gaaaccagaa gatttccgcg ccagtaccca atgtcctttc accggggaag agtatctgaa
      901 aagcctgcag gatggtcgcg agatctatat ctatggcgag cgagtgaaa
//
LOCUS       DADKXM010000330          944 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.333, whole genome shotgun sequence.
ACCESSION   DADKXM010000330 DADKXM010000000
VERSION     DADKXM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.333"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>944
                     /locus_tag="JQR05_004214"
     CDS             <1..>944
                     /locus_tag="JQR05_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HAY5326139.1"
                     /translation="RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRT
                     PDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLI
                     EQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKL
                     KTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEI
                     ELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQ
                     LSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQL"
BASE COUNT          257 a          234 c          280 g          173 t
ORIGIN      
        1 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
       61 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      121 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      181 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      241 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      301 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      361 gggactgtta aatgcctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      421 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      481 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      541 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      601 catgcgcttc cccggaggag aaatggcgga tctgtacagt catcgctggg aaatcgaact
      661 gggatacagg gagataaaac agacgatgca actgagcagg ctgacgctga gaagtaaaaa
      721 gccggagctt gtggagcaag agctgtgggg tgtcttactg gcttataatc tggtgagata
      781 tcagatgatt aaaatggcgg aacatctgaa aggttactgg ccgaatcaac tgagtttctc
      841 agaatcatgc ggaatggtga tgagaatgct gatgacattg cagggcgctt caccgggacg
      901 tataccggag ctgatgcgcg atcttgcaag tatgggacaa cttg
//
LOCUS       DADKXM010000331          937 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.334, whole genome shotgun sequence.
ACCESSION   DADKXM010000331 DADKXM010000000
VERSION     DADKXM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.334"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..269)
                     /locus_tag="JQR05_004215"
     CDS             complement(<1..269)
                     /locus_tag="JQR05_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY5326140.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVA"
     gene            complement(371..925)
                     /locus_tag="JQR05_004216"
     CDS             complement(371..925)
                     /locus_tag="JQR05_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5326141.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
BASE COUNT          249 a          265 c          224 g          199 t
ORIGIN      
        1 aacgcgacaa taccggttat gacataaccg agaatttcca gatgtggtgc cagtgcacta
       61 ccgtaaataa tgaccatcgc ccacaccacg ccactaagca gggttgcagc ggagatcgcc
      121 agccctttca gcccaccttg cgggcaagca aagtaggccg tacagcccag gaagcccgcc
      181 cagcttagta agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt
      241 gtgattgcaa tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg
      301 aatgagatcg agcacacatt ttatgcaaca acaaattatc tgttgcagtt atgagtgacg
      361 atgatcacat ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatggcat
      421 cacgggttag cggtgccatg gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc
      481 cgaggaacgc accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc
      541 tctcatcttc cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt
      601 gatcggctgg cgtatccgcc agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg
      661 cttccgttgc gtcctcaacc catgcgctac gatatggcgg cacggcacat tcatcgccga
      721 taaacaacgc attgtaatcg gcagagactt gcgtcatatc acagcttttc tgtaaacgcg
      781 tcagtaactc atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcagag
      841 taaacagcgg caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac
      901 gacagaggat agaaaactcg ttcattaata tatttcc
//
LOCUS       DADKXM010000332          910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.335, whole genome shotgun sequence.
ACCESSION   DADKXM010000332 DADKXM010000000
VERSION     DADKXM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.335"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..446)
                     /locus_tag="JQR05_004217"
     CDS             complement(<1..446)
                     /locus_tag="JQR05_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY5326142.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAW"
     gene            complement(397..>910)
                     /locus_tag="JQR05_004218"
     CDS             complement(397..>910)
                     /locus_tag="JQR05_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326143.1"
                     /translation="TRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCY
                     WPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLV
                     RGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSG
                     AMPGLFIRTS"
BASE COUNT          193 a          251 c          231 g          235 t
ORIGIN      
        1 ctccacgcca tatgtgtttg tgctgtgaca gattttgcca gcatgactac cggggacagg
       61 cggcccagtg agtcgggacc agcattccag atacgaccgt tccattcaaa cgtgaacggc
      121 ttcgcctcct gttctgcgcg ccatgcttca atttcctgac gtctggcctc tctggccgct
      181 tccagcattt ctggtgtcac agtgaatggg gctatctcac cccatttgcc actttgcagt
      241 tcctgccaga ttcgctgacc cgtcggtgcg acatcatcag cggtggctgt gtaggggact
      301 gcctggtccc tgtcgtcaaa aaaaacgtca cagtctactg cgccactttc ggtataacgg
      361 ggattaatga tttttttaat ttccacggtg cattcctcac gatgtgcgaa taaaaagccc
      421 gggcattgcg ccagagacat gtgcatccgg caccccggac agggcgcaat atgaccccgg
      481 taatgaatgc tctgaacatc ccgtaatgaa aaattgtggg gatgctatat acgttccggt
      541 gggagtactg ggcactgaaa tccccaccgg tcccagtcgt gagcctctgt atgactgccc
      601 cctgacaagt ctgatgactt tatcaccgtc agcttctccc tggtacgcag caataatcag
      661 cccgccaatg tcagggtctc cccatctgtt gcggacagag ctcgccacga ttctgtaaat
      721 aatatcttct gtggttatat ttattttcac ccagccagtc agtctggata tgggccagta
      781 gcagtagcgg gtgtgataaa tggggccgtt aatgccgtaa aaagtaaggg atttggctct
      841 gtacctcggt tctgttgtct cagggcgtgc atcagtccac cggatgctga gcaccccttc
      901 aaaccgtgtg
//
LOCUS       DADKXM010000333          896 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.336, whole genome shotgun sequence.
ACCESSION   DADKXM010000333 DADKXM010000000
VERSION     DADKXM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.336"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..829)
                     /locus_tag="JQR05_004219"
     CDS             complement(<1..829)
                     /locus_tag="JQR05_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY5326144.1"
                     /translation="MASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDW
                     LDSHGFFWASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQA
                     TGREAAFGESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPP
                     GLLRSVAITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKL
                     GGLIRFFHGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIR
                     "
BASE COUNT          190 a          279 c          217 g          210 t
ORIGIN      
        1 accgtatggc cattgccgca cccggataag gcaggttcgc cagcacccgc ggaatatagt
       61 tgcgggtctc ctccggagca gcagccagtc ccttacgctg aacatttccc tctccccagt
      121 tatatgccgc cagagcctta gccagatcgc catgaaaaaa acgtatcagg ccaccaagtt
      181 ttctcgcggc ggcatcagcg gctttttcgg gatcaaaggc atcgttcccc ctcagaccaa
      241 attcactggc cgtcttcggc atgaactgaa acagtcccat cgcaccagca ggtgagacgg
      301 caaactgatt accacgggat tcggtgatcg caacgctgcg cagcagtccg ggcggcaggt
      361 tatatttttc ctccagttga gacagtttcg gttgcagcca gcctaacagg gcttccccgg
      421 tcttcgtcgg tcgctggcgg ttttgcatgg cattaccgag tttttcctgc gtcgcacgca
      481 taccctgtag ccaggattca ccgaaggctg cttccctccc ggttgcctga gaagcctgag
      541 tatccagcat gccctgctgc catactgtgg gtgattgtgc ccgggtgatg ttgccaggct
      601 tttctccggc atccagtttt gtctgatacg tctccatctc tttcttattg aaaaagaaag
      661 tcccgtctga agcccagaaa aaaccatgtg aatccagcca gtccttattt tccctgccaa
      721 caatggatgt cagtaatccg tcaacaaccg ggtaaagcgc cgttatcgca aaaagaaggc
      781 ctccggggcc actgagggct tttgtcagcc cccgcaccca tgacgccact ttcagcccga
      841 tcagcgtaat aatgacattc tgccagcccc ccagttctcc ggcagcctta ttcgcc
//
LOCUS       DADKXM010000334          866 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.337, whole genome shotgun sequence.
ACCESSION   DADKXM010000334 DADKXM010000000
VERSION     DADKXM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.337"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            72..>866
                     /gene="ltrA"
                     /locus_tag="JQR05_004220"
     CDS             72..>866
                     /gene="ltrA"
                     /locus_tag="JQR05_004220"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY5326145.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHHLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHC"
BASE COUNT          209 a          206 c          269 g          182 t
ORIGIN      
        1 acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa tggtacaggg acggagtaaa
       61 ccgtagtagc ggtgaaggac cgtaatgggt ccggagcaaa gggcttgccc cattgtgctg
      121 atggtgcggc ggcaaccacg ggcgacaatg ccgatgggag gactgctgta aaatcggcaa
      181 agccatttcc tgtaagtaaa cgacaggtat gggaagctta taaacgagta aaagccaacc
      241 gtggggcggc aggcattgac gggcagacac tggccgggtt tgacgagaat gttacagaca
      301 atctctacaa actgtggaac agaatggctt ccggcagtta tatgccgcag gcggtaaggc
      361 gtgtggacat tcccaaagct gacggtggtg tacgtccgct cggtattccg gccgtgtcgg
      421 accggatcgc ccagatggtc gttaagcaga tactcgagcc ggtactggaa cccctgttcc
      481 atgcagactc gtatggttat cgaccgggta aatccgcaca ccaggcgatt gcgcaggcga
      541 ggaaacgctg ctggaagttt gactgggtgg tggaagtcga tattaaaggc ttctttgatg
      601 atatcgatca tcacctgcta ctgaagacag tgcagcatca tactcaggca cgctgggtgg
      661 tgatgtacat tgagcgcagg ctgaaggcac cggtgcaaat gccggatgga gcaatgctgg
      721 cacggggtcg aggaacgccg cagggcggcg taatctcccc cctgctctca aatctgttcc
      781 tgcactatgc ctttgatatg tggatgcaac gtcagtttcc cggcgttccc ttcgaaaggt
      841 acgccgatga tgttgtatgc cactgt
//
LOCUS       DADKXM010000335          865 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.338, whole genome shotgun sequence.
ACCESSION   DADKXM010000335 DADKXM010000000
VERSION     DADKXM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.338"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..361
                     /locus_tag="JQR05_004221"
                     /pseudo
     CDS             1..361
                     /locus_tag="JQR05_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
     gene            271..498
                     /locus_tag="JQR05_004222"
     CDS             271..498
                     /locus_tag="JQR05_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY5326146.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            476..772
                     /locus_tag="JQR05_004223"
     CDS             476..772
                     /locus_tag="JQR05_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY5326147.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          281 a          189 c          220 g          175 t
ORIGIN      
        1 agcgacccga atgattcgaa atggattccc agtcatcata tctcctcaaa tgaagtcaaa
       61 aaaacggaaa atgaagtgcg acagactgaa gataaacagc accagaacag cgaaccggag
      121 gagggaacac cctgcaatca gactggcgag gataactgcc ctgactgtgg cgcggtgatg
      181 ggagacgcaa cttatcagga aacttttgac gaaaaaaaac cagatgaagc acaggaagaa
      241 gagccgaaga aaacggaaaa agctgatgat ttgttaccgg agaacgctgg cagcgatcag
      301 cataatgata gcaataatga aactggcgaa gatgaactca actggaaaaa acaggtgttg
      361 attgcggcgg tttacggttt atgtgcgaat cctgcgtgca tagcctatgc gcctgcaatc
      421 cctgacatcg caatgatgat tgccaacaag cttgaaaatt ttggagtgac cagtgatgag
      481 cgcttacccg atttttgatc gtattgaaga aatggcatgg gctcgccact accagcagat
      541 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      601 cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat
      661 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      721 catggctgaa accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg
      781 gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt
      841 gctggcgacg gcaaaaccta cacag
//
LOCUS       DADKXM010000336          852 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.339, whole genome shotgun sequence.
ACCESSION   DADKXM010000336 DADKXM010000000
VERSION     DADKXM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.339"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            142..>852
                     /locus_tag="JQR05_004224"
     CDS             142..>852
                     /locus_tag="JQR05_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY5326148.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGT"
BASE COUNT          239 a          189 c          218 g          206 t
ORIGIN      
        1 gtcgactaac aaaaaatggc tgtttgtgaa aaaaattcta aaggttgttt tacgacagac
       61 gataacaggg ttgacggcga ttgagccgac gggtggaaac ccaaaacgta atcaacgact
      121 tgcaatatag gataacgaat catggcacaa gtcattaata ccaacagcct ctcgctgatc
      181 actcaaaata atatcaacaa gaaccagtct gcgctgtcga gttctatcga gcgtctgtct
      241 tctggcttgc gtattaacag cgcgaaggat gacgccgcgg gtcaggcgat tgctaaccgt
      301 tttacttcta acattaaagg cctgactcag gctgcacgta acgccaacga cggtatttct
      361 gttgcacaga ccactgaagg cgcgctgtcc gaaatcaaca acaacttaca gcgtatccgt
      421 gagctgacgg ttcaggcttc taccgggact aactctgatt cggatctgga ctccattcag
      481 gacgaaatca aatcccgtct cgacgaaatt gaccgcgtat ccggtcagac ccagttcaac
      541 ggcgtgaacg tactggcaaa agacggttcg atgaaaattc aggttggtgc gaatgacggc
      601 cagactatca ctattgatct gaagaaaatt gactctgata cgctggggct gaatgggttt
      661 aatgtgaatg gtggcggggc tgtggctaat actgctgcat ctaaagctga cttggtagct
      721 gctaatgcaa cagtggtcgg caacaaatat actgtgagtg cgggttacga tgctgctaaa
      781 gcgtctgatt tgctggctgg agttagtgat ggtgatactg ttcaggcaac cattaataac
      841 ggcttcggaa cg
//
LOCUS       DADKXM010000337          820 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.340, whole genome shotgun sequence.
ACCESSION   DADKXM010000337 DADKXM010000000
VERSION     DADKXM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.340"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..215
                     /locus_tag="JQR05_004225"
     CDS             <1..215
                     /locus_tag="JQR05_004225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326149.1"
                     /translation="CADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIP
                     AGISADGMYCPECGTVHWPDGVIPPF"
     gene            263..692
                     /locus_tag="JQR05_004226"
                     /pseudo
     CDS             263..692
                     /locus_tag="JQR05_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          203 a          164 c          263 g          190 t
ORIGIN      
        1 gttgcgcaga cctgaatatt ccgttctttg tggatgccgg aaacaaaaaa ctggtcttct
       61 ggtttgtacg tgttgatgac gaagggtatc cggaaattgc ccgctgcacg gagcgggaat
      121 ttgcgacaat tcctgccggt atcagcgccg acggcatgta ttgccctgaa tgcggtacgg
      181 ttcactggcc ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt
      241 tcagcaggga aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct
      301 ggaacattat gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg
      361 agaagaaaac cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc
      421 ggataagggg ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg
      481 aagatgtggt gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga
      541 gagggatgta tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc
      601 agatgcggtt tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg
      661 agccgctggc tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg
      721 ttgaggggaa cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata
      781 ttaagtgtct ataccgatta cttgatgatc aggttgtttt
//
LOCUS       DADKXM010000338          798 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.341, whole genome shotgun sequence.
ACCESSION   DADKXM010000338 DADKXM010000000
VERSION     DADKXM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.341"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..668)
                     /gene="pdeC"
                     /locus_tag="JQR05_004227"
     CDS             complement(<1..668)
                     /gene="pdeC"
                     /locus_tag="JQR05_004227"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY5326150.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRP"
BASE COUNT          236 a          173 c          172 g          217 t
ORIGIN      
        1 gggcgttttg tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc
       61 ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag
      121 gttttcgtca ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca
      181 ctccagaaga gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta
      241 tagccagaga aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta
      301 taatcggccg gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa
      361 tggttatcac gggcatagat caattcatta atataaagat agccacgaat aatattcaac
      421 attcgctttt gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc
      481 actaaatctg cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt
      541 cggagctgat tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg
      601 atgggaaaga gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga
      661 tgactcattg ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaccctg
      721 gctaccgacg tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat
      781 tgataccttt ttaatcgt
//
LOCUS       DADKXM010000339          785 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.342, whole genome shotgun sequence.
ACCESSION   DADKXM010000339 DADKXM010000000
VERSION     DADKXM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.342"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..785
                     /locus_tag="JQR05_004228"
                     /pseudo
     CDS             1..785
                     /locus_tag="JQR05_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          183 a          194 c          240 g          168 t
ORIGIN      
        1 taagcaatac caggaaggaa gtcttactgc tgtggccgcc ggagaacagg ttgttcctgc
       61 ctctgaactt gctgccgcca tgaagcagat taaagaactc cagcgcctgc tcggcaagaa
      121 aacgatggaa aatgaactcc tcaaagaagc cgttgaatat ggacgggcaa aaaagtggat
      181 agcgcacgcg cccttattgc ccggggatgg ggagtaagct tagtcagccg ttgtctccgg
      241 gtgtcgcgtg cgcagttgca cgtcattctc agacgaaccg atgactggat ggatggccgc
      301 cgcagtcgtc acactgatga tacggatgtg cttctccgta tacaccatgt tatcggagag
      361 ctgcccacgt atggttatcg tcgggtatgg gcgctgcttc gcagacaggc agaacttgat
      421 ggtatgcctg cgatcaatgc caaacgtgtt taccggatca tgcgccagaa tgcgctgttg
      481 cttgagcgaa aacctgctgt accgccatcg aaacgggcac atacaggcag agtggccgtg
      541 aaagaaagca atcagcgatg gtgctctgac gggttcgagt tctgctgtga taacggagag
      601 agactgcgtg tcacgttcgc gctggactgc tgtgatcgtg aggcactgca ctgggcggtg
      661 actaccggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc ggtggaacgc
      721 cgcttcggca acgatcttcc gtcgtctcca gtggagtggc tgacggataa tggttcatgc
      781 taccg
//
LOCUS       DADKXM010000340          781 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.343, whole genome shotgun sequence.
ACCESSION   DADKXM010000340 DADKXM010000000
VERSION     DADKXM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.343"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..689
                     /locus_tag="JQR05_004229"
                     /pseudo
     CDS             1..689
                     /locus_tag="JQR05_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          208 a          165 c          189 g          219 t
ORIGIN      
        1 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
       61 cagtttgaac tagacctgga agcatcaaac agacacaaca ctgaccaaac tcgtttggca
      121 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      181 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      241 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      301 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      361 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaacggt ttctcatttg
      421 ctggtgctta tgcaaactct gaccgtactg attggcagtc tggtgatggt aaaggcgaaa
      481 gagccgaagt ttgggcgttg tcaacaaaat atgacgctaa caatgtttat gctgctgtga
      541 tgtacggtga atctcacaac atgaattccg acgacggtga tgttgtgaac aaaacacaaa
      601 acttcgaagc tgttctgcaa tatcagttcg actttggtct gcgtccatcc atcggttact
      661 cctattctaa agcactggac gtagcaggtt ggatttgccc ctatatttcc agacacctgt
      721 tatcacttaa cccattactg gcttgctgcc gtagatattc ccgtggcgag cgataaccca
      781 g
//
LOCUS       DADKXM010000341          764 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.344, whole genome shotgun sequence.
ACCESSION   DADKXM010000341 DADKXM010000000
VERSION     DADKXM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.344"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(683..757)
                     /locus_tag="JQR05_004230"
     tRNA            complement(683..757)
                     /locus_tag="JQR05_004230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(722..724),aa:Gly,seq:tcc)
BASE COUNT          233 a          190 c          158 g          183 t
ORIGIN      
        1 atcccgcccc cccgacagac ctgtgctatc ggaaagaaaa aaggccatca gcaaaactct
       61 gatggcctga atcaccttta ccaatattgt atgaaaaaac acgcacgact aattgacaat
      121 aattttcatt tccattaaaa aagatcgcgt agcattctta attcatgagg aactttaccc
      181 gccagcaatc tgagtagcga aagctgttca tccccaatgt ttttgctggc gggtcctttt
      241 ttctacggcc ctcctccccg gagggggggc atttcattat ttttctaatc gttactggtg
      301 agaaggcgga agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg
      361 caaaatctga tttccagctc acacataccg agcactgtac aaaaaaccgc caaccaaggc
      421 agttaagagt gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat
      481 ttacaacacc agaatgatgc attatcagtc ccctgccaga ggttcactac gtaacctgac
      541 cgacaatgca ccattctggt gctgaaaaaa gagcactgga actgcaacaa aacaacatca
      601 cagaacagga gatggtaagg agtcagacat tcccacacaa tattgtgtca gtgcaaataa
      661 caaccttcgt ctcaaatctc gctggagcgg gcagcgggaa tcgaacccgc atcatcagct
      721 tggaaggctg aggtaatagc cattatacga tgcccgcata tggt
//
LOCUS       DADKXM010000342          756 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.345, whole genome shotgun sequence.
ACCESSION   DADKXM010000342 DADKXM010000000
VERSION     DADKXM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.345"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..347
                     /locus_tag="JQR05_004231"
     CDS             <1..347
                     /locus_tag="JQR05_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY5326151.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          218 a          182 c          167 g          189 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca
      481 tgtgttttct ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgta gagggttttt atgcgtattg aaatctgcat
      721 agccaaagag aaaatgacta aaatgccaac cggtgc
//
LOCUS       DADKXM010000343          752 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.346, whole genome shotgun sequence.
ACCESSION   DADKXM010000343 DADKXM010000000
VERSION     DADKXM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.346"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..488)
                     /locus_tag="JQR05_004232"
     CDS             complement(<1..488)
                     /locus_tag="JQR05_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY5326152.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QK"
     gene            complement(635..711)
                     /locus_tag="JQR05_004233"
     tRNA            complement(635..711)
                     /locus_tag="JQR05_004233"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Pro,seq:tgg)
BASE COUNT          185 a          192 c          187 g          188 t
ORIGIN      
        1 tgtttttgca ggagcgtcag ggcacgcatc accagtttat gcgtgggtct gccgcctttg
       61 gtcacgcgat attgattgtg gatctcagcc ggaccatcca gcgataaccc aacaagaaaa
      121 ttattttctg ccagaaatgc acaccattcg tcatcgagca gcacgccgtt agtctggaaa
      181 ctgttactta tcttcctgcc agcaccatat ttcgcctgta gcgccacggc acggcggtaa
      241 aactccagcc cgagtagcgt tggttcgccg ccctgccagg taaaagccac ttcgttttgc
      301 agttcgctgg cggcgatata gtgacggata tacgcttcca gcgtgtcatc gtccatatgc
      361 gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta gtcacagttc
      421 agattacaat cggaaccgct cggtttcgcc atcacatgaa aagcacgcgt tggaacctgt
      481 tgcagcatgg tcgctccttg cctgaagtca tccttcaata cgttaagggc gaaatcatac
      541 cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg agggtgacga aatgcacaga
      601 aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg attcgaacct
      661 ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc gaaatactgc
      721 tttttgaatt tttagttcaa ttctttaaag tc
//
LOCUS       DADKXM010000344          745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.347, whole genome shotgun sequence.
ACCESSION   DADKXM010000344 DADKXM010000000
VERSION     DADKXM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.347"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..341
                     /gene="dgcP"
                     /locus_tag="JQR05_004234"
     CDS             <1..341
                     /gene="dgcP"
                     /locus_tag="JQR05_004234"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY5326153.1"
                     /translation="LFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLR
                     ERIQQQIRGEYHLGDVDLYYPSASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKT
                     PFVAHPALHS"
     gene            374..472
                     /gene="yoaK"
                     /locus_tag="JQR05_004235"
     CDS             374..472
                     /gene="yoaK"
                     /locus_tag="JQR05_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY5326154.1"
                     /translation="MRIGIIFPVVIFITAVVFLACFFIGGYAAPGA"
     gene            475..549
                     /gene="yoaJ"
                     /locus_tag="JQR05_004236"
     CDS             475..549
                     /gene="yoaJ"
                     /locus_tag="JQR05_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY5326155.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT          193 a          155 c          179 g          218 t
ORIGIN      
        1 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
       61 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
      121 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
      181 ttgatttgta ttatcccagt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
      241 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
      301 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
      361 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
      421 cgtattttta gcatgttttt ttattggcgg ctatgctgcc ccgggagcat aaagatgaaa
      481 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
      541 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
      601 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
      661 ctgaagcggc gcacgatcgt aatcgtgcgc ttccagcgtg gggcgggcga tttcaaaata
      721 cccctggctt gtttctattt gctca
//
LOCUS       DADKXM010000345          728 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.348, whole genome shotgun sequence.
ACCESSION   DADKXM010000345 DADKXM010000000
VERSION     DADKXM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.348"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(439..>728)
                     /locus_tag="JQR05_004237"
     CDS             complement(439..>728)
                     /locus_tag="JQR05_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5326156.1"
                     /translation="IGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQ
                     GFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
BASE COUNT          222 a          129 c          132 g          245 t
ORIGIN      
        1 cacttcaaag cataatctat cgttcatttt ctgagcctgt aaaacatgtg gtgatatagt
       61 ctgagagcat aaaagttgag attctgtgta tcaagtcctg tcttggcacc gaatgaatat
      121 tgcttacgaa gccccttcat gctttccttg agaacaataa ttttatcttt atgtgaatct
      181 cattttttat aatgatgtca gatgattgaa atgctaaatt atatctgaat caacttttcc
      241 tttgctaaag gtatcgaaat aactgcaagg ctgtattttg taattgccaa tttaaaaatg
      301 tcaatttgtt tttaattatg tttttgtaat aagtttgaag acgcaaaaaa ataatataat
      361 aacaaaaatc ctccagcctt gtaggccaga ggataaaata cttaattgag gaaaacatca
      421 gggaaaagta tttctgactt aactttcccg gctggagagt gaatcaatgt aatcagcgta
      481 ccattgcatc atttcacggc ggccatcgag atacagggca tgattatagg tacccctgat
      541 ggaattttta tccacatgag ctaactgcat ttcaatccag gcggaattaa agccttgttc
      601 atgcagaatt gtactcatgg tgtgtctgaa accgtgccct gtgagtcttc ccttatatcc
      661 aagtaattca attacttggt taatgctttc cttactaatt ggtttgcggg gatcatttct
      721 accgataa
//
LOCUS       DADKXM010000346          724 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.349, whole genome shotgun sequence.
ACCESSION   DADKXM010000346 DADKXM010000000
VERSION     DADKXM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.349"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(246..>724)
                     /gene="sseA"
                     /locus_tag="JQR05_004238"
     CDS             complement(246..>724)
                     /gene="sseA"
                     /locus_tag="JQR05_004238"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY5326157.1"
                     /translation="AGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHEN
                     TPQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFG
                     RGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT          185 a          197 c          160 g          182 t
ORIGIN      
        1 tcggactaat aagccaggtt attttattac cgaacacggc ggaaagggcc ggtgtcgata
       61 atacgctaaa taaacaataa ttactctctt ttgcttgaca aaaagagagt tactggtgag
      121 tattgttttg ctgtgaagca cctgcgttgc tcatgtaaca caagtcaacc tgctggaaag
      181 gccgcgaacc agaccagcaa taaaaaaacg ccaaattcgg cggtttttta ttgcctgtaa
      241 aatacttatt tcactggctc aaccggtaaa tctgcccgcg cgccccattc actccatgcg
      301 ccgtcgtaca gtttcacgtt tggcacatcc agcgtcgcga gtgctaacaa aaccacggct
      361 gccgttacac cagagccgca gctgacgata atcggtttgt catagctgac gccgcgacca
      421 aaaaatatcg catccagttc atcggtcgtt tttagttcgc cttcgcgcac cagttccgtc
      481 cacggaacgt tcaatgcacc gggaatatgt ccgcgacgta aacctgggcg aggttcatca
      541 acttctgcgt taaaacgtgc agccgggcgg gcatcaataa tttgcggcgt attttcatgg
      601 cttgccaata acacatcggt tactttcacc actgcttcag gattaaacgc ggcgttaaac
      661 tctccttccg gcagctctac tgcaccttct tctaatagca ggtcatcgcg ctgccagcct
      721 gcaa
//
LOCUS       DADKXM010000347          702 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.350, whole genome shotgun sequence.
ACCESSION   DADKXM010000347 DADKXM010000000
VERSION     DADKXM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.350"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            57..143
                     /locus_tag="JQR05_004239"
                     /pseudo
     CDS             57..143
                     /locus_tag="JQR05_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(153..233)
                     /locus_tag="JQR05_004240"
                     /pseudo
     CDS             complement(153..233)
                     /locus_tag="JQR05_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            444..533
                     /locus_tag="JQR05_004241"
                     /pseudo
     CDS             444..533
                     /locus_tag="JQR05_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            542..>702
                     /locus_tag="JQR05_004242"
     CDS             542..>702
                     /locus_tag="JQR05_004242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326158.1"
                     /translation="MSLSLVGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEA
                     KVRLEAPGR"
BASE COUNT          185 a          146 c          167 g          204 t
ORIGIN      
        1 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
       61 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      121 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
      181 caccactgca tcatttctct gcgcttatcg agatactgag catggttgta aattcaacta
      241 acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa aatcaccttg cgctaatgct
      301 ctgtatcagg tcactaatac cagctaagta gttgattcat agtgactgca tatgttgtgc
      361 tttgtcgcat tttgtagtct attatttaat ctgtaacaac gataatacaa tgaattttaa
      421 ttggtttttg tgattttgtc cttcttctga catggaagga cacccgatgg atacccaacg
      481 attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga tagcagcgtg
      541 cttgtccctg tctcttgtgg gaacagggct tgctaaagct gatgattcgc ttccttccag
      601 taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca gttttagcag
      661 cagtgaagag gcgaaagtgc gactggaagc gccggggcgt ga
//
LOCUS       DADKXM010000348          696 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.351, whole genome shotgun sequence.
ACCESSION   DADKXM010000348 DADKXM010000000
VERSION     DADKXM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.351"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..473
                     /locus_tag="JQR05_004243"
     CDS             <1..473
                     /locus_tag="JQR05_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5326159.1"
                     /translation="GGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGET
                     ATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAK
                     VCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          172 a          162 c          230 g          132 t
ORIGIN      
        1 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
       61 cctcccggct ggcacattac aacggagggg cgagcctgag cttccgttac ctggaccaca
      121 aaacgggaga aacggcgacg gaaacgctga cacagcgtga gctggtcgcg aggctgaaac
      181 agcacatccc ggagaagttt tttaagatgg tgaggtactt cgggttcctt gccaaccggg
      241 tgtgtggaga gaagctgccg caggtgtacc gtgctctggg gatggataaa ccggaaccag
      301 tggcgaaggt gtgctatgca caaatggtga agcagttcct gagtcgtgac ccgttcgaat
      361 gcgtgctgtg tggcggccgg atggtatacc gccgggccat tgcggggctg aatgtggagg
      421 ggctgaagaa aaacgcgcgg gatatcagtc tgctgaggta tatgccggcc tgacacaggc
      481 taagtgtgcc cgggaatcgg tgcaagtata aaaaacagcc tgttgcggta taagaatcga
      541 ccatgcagag agagaaaaca ccggagtgga gggaaaagca gaaaagctca cgtggcataa
      601 ggcgtggaca gcggtatcgt ctggtttttc aatttcctat acgtgaacta tggctcgcta
      661 cgaccttccc gatgaggcat ggaccatcat caagcc
//
LOCUS       DADKXM010000349          690 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.352, whole genome shotgun sequence.
ACCESSION   DADKXM010000349 DADKXM010000000
VERSION     DADKXM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.352"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            27..690
                     /locus_tag="JQR05_004244"
                     /pseudo
     CDS             27..690
                     /locus_tag="JQR05_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          155 a          178 c          193 g          164 t
ORIGIN      
        1 tataatggtg tttttgaggt gctcccgtgg cttcagtctc cgtctgctgt ccctcctgtt
       61 ccgctactga aggcgtgatg cgtaatggca aaagtactgc cgggcatcaa cgttatctct
      121 gctctcactg ccgtaaaaca tggcagctca ccttcactta tgccgcttct cagcccggta
      181 cacaccagaa aatcattgat atggctatga acggcgtcgg ttgccgtgcc accgcacgac
      241 taatgggcgt gggcctcaac accattttgc gccatttaaa aaactcaggc cgcagtcagt
      301 aacctcccgg atacagccgg gcagtgatgt cattgtctgt gcggagatgg acgagcaatg
      361 gggctacgtc ggtgcaaaat cacgtcagcg ctggctgttt tacgcgtatg acaggatacg
      421 cagggcggtg gtagcccacg ttttcggtga acgcactatg gccacactgg agcgtcttct
      481 gagcctgctg tcagtttttg acgtggtggt atggatgacg gacggctggc cgatgtatga
      541 gtcacgtctg aagggaaaac tgcacgtcat cagtaagcgt tacacgcagc gaattgagcg
      601 acacaacctg aatctgagac agcatctggc aaggctgggc aggaagtccc tgtcgttctc
      661 aaaatcggtg gagctgcatg acaaagtcat
//
LOCUS       DADKXM010000350          685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.353, whole genome shotgun sequence.
ACCESSION   DADKXM010000350 DADKXM010000000
VERSION     DADKXM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.353"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            67..282
                     /gene="xisR"
                     /locus_tag="JQR05_004245"
     CDS             67..282
                     /gene="xisR"
                     /locus_tag="JQR05_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY5326160.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            284..>685
                     /locus_tag="JQR05_004246"
     CDS             284..>685
                     /locus_tag="JQR05_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAY5326161.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNY"
BASE COUNT          231 a          126 c          158 g          170 t
ORIGIN      
        1 cctcattaat aatcaacatc gggcgtcaat ttcagcctaa cattggcgcc tgccagaggt
       61 gatgcgatgg cacaagtaat ctttaatgaa gagtggatgg ttgaatacgg cctgatgctt
      121 cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg ggtggaaggc
      181 ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg gattatctgg
      241 tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta aattaccaac
      301 aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag gaaaacgatg
      361 tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg ccgggaattt
      421 aagagcgttg atagttcatg aaatcagttc cggtgaattt gagtatttaa gacgttttcc
      481 ccagtccagc actggggcaa aaatggtgac aacgagggtc ataaaaacat tcggggagct
      541 ttgtgatatc tggacaaaaa ttaaagagac agagttaaca acaaacacaa tgaagaaaac
      601 gaaatcacaa ttaaaaacac tcaggataat aatttgtgag agtaccccga tatcgcatat
      661 tcgttatagc gatatcttaa actac
//
LOCUS       DADKXM010000351          682 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.354, whole genome shotgun sequence.
ACCESSION   DADKXM010000351 DADKXM010000000
VERSION     DADKXM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.354"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..682
                     /locus_tag="JQR05_004247"
                     /pseudo
     CDS             1..682
                     /locus_tag="JQR05_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          168 a          176 c          185 g          153 t
ORIGIN      
        1 aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc gatatcacca
       61 cccacagcct ttatgcctgg ataaagaagt acggtccgga ttcttccact aataaagaac
      121 agtcagatgc tcaggccgag atccgccgtc tccagaaaga gctgaaacgg gttaccgacg
      181 aacgggacat attaaaaaaa gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc
      241 ctttatccgt gacaactcct gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt
      301 tcatcccagt ggtttttacg cctggcttca gcagccgcat tcacaacgcc atcaggcaga
      361 cctgagactg acaggacaga ttaaacagtt ctggctggaa tcgggatgcg tctatggtta
      421 tcgcaaaatc catctggatc tgcgtgacag cgggcaacag tgcggagtaa acagagtctg
      481 gagactgatg aaacgtgtcg gaataaaggc tcaggtcgga taccgaagcc cgcgggcacg
      541 taaaggcgag gccagtatcg tgtcacccaa caggctccag cgacagttca atccggatgc
      601 tcctgatgag cgttgggtaa cggacataac ctacatcagg acccacgaag gctggctgta
      661 tcttgccgtt gttgttgatc tg
//
LOCUS       DADKXM010000352          673 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.355, whole genome shotgun sequence.
ACCESSION   DADKXM010000352 DADKXM010000000
VERSION     DADKXM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.355"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            84..>673
                     /locus_tag="JQR05_004248"
     CDS             84..>673
                     /locus_tag="JQR05_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY5326162.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQL"
BASE COUNT          175 a          178 c          175 g          145 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgctcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagaacatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca act
//
LOCUS       DADKXM010000353          665 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.356, whole genome shotgun sequence.
ACCESSION   DADKXM010000353 DADKXM010000000
VERSION     DADKXM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.356"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..550
                     /locus_tag="JQR05_004249"
     CDS             <1..550
                     /locus_tag="JQR05_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5326163.1"
                     /translation="DFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIA
                     MGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFS
                     QKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQI
                     KGESYRLRQKRKAGVIAEANPE"
BASE COUNT          187 a          163 c          164 g          151 t
ORIGIN      
        1 tgacttcaca ttcgccaccg gagcaccgca gaagcaactc cagtcgttac gctcactcag
       61 cttcatagaa cgtaatgaaa atatcgtatt actggggcca tcaggtgtgg ggaaaaccca
      121 tctggcaata gcgatgggct atgaagcagt ccgtgcaggt atcaaagttc gcttcacaac
      181 agcagcagat ctgttacttc agttatctac ggcacaacgt cagggccgtt ataaaacgac
      241 gcttcagcgt ggagtaatgg ccccccgcct gctcatcatt gatgaaatag gctatctgcc
      301 gttcagtcag aaagaagcaa aactgttctt ccaggtcatc gctaaacgtt acgaaaagag
      361 cgcaatgatc ctgacatcca atctaccgtt cgggcagtgg gatcaaacgt tcgccggtga
      421 tgcagcactg acctcagcga tgctgggccg tatcttacac cactcacatg tcgttcaaat
      481 caaaggagaa agctatcgac tcagacagaa acgaaaggcc ggggttatag cagaagctaa
      541 tcctgagtaa aacggtggat caatattggg ccgttggtgg agatataagt ggatcacttt
      601 tcatccgtcg ttgacacaac actggctctg gtatgcgtac aacactaaaa ctggcggtgt
      661 gttgg
//
LOCUS       DADKXM010000354          660 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.357, whole genome shotgun sequence.
ACCESSION   DADKXM010000354 DADKXM010000000
VERSION     DADKXM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.357"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            246..524
                     /locus_tag="JQR05_004250"
                     /pseudo
     CDS             246..524
                     /locus_tag="JQR05_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            584..>660
                     /locus_tag="JQR05_004251"
     CDS             584..>660
                     /locus_tag="JQR05_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5326164.1"
                     /translation="MTKQYDVTWLFSEDEKNWYEELKNFA"
BASE COUNT          197 a          124 c          169 g          170 t
ORIGIN      
        1 ctggcggccg ggttaaagac ctgatgaatc tcaaatggga tacactaaat attctggtta
       61 atgccgtcat taatggttgt cttggaattg gtacgacgaa tgcgttaggt ggaagttcaa
      121 tcgttttagg agataacgat accgggttta aacaaaatgg cgatggtctg cttgatgttt
      181 atgcgaatgg acagcgtgta tttcgtttcc agaatggcag tatccagagt aataaagctg
      241 taaacgttac aggacgggta acaccgtcag actacggaaa ctttgatgcc cgttaccagc
      301 agcgaaatgg tggcgtgcag gatgtgcggt atggttatga aatgtattac aaccctggca
      361 gcaacaccgt ttcatggacg tttcgctcac cttcgggaca cgggctgtca gggatatcga
      421 tatcggatac cggccgtaac tcagcggata acgtcaacgg tgtgtattac cgaccgctgc
      481 aaaaactgat taatggcacc tggtataacg tagcgagtat ttaacaatgt tgcatttaaa
      541 aaatattact gcgggtaatc cgaaaacagc agaacaatat cagatgacaa aacagtatga
      601 tgtcacctgg cttttttcgg aagacgaaaa aaactggtat gaagagctga agaatttcgc
//
LOCUS       DADKXM010000355          652 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.358, whole genome shotgun sequence.
ACCESSION   DADKXM010000355 DADKXM010000000
VERSION     DADKXM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.358"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /gene="yqeC"
                     /locus_tag="JQR05_004252"
     CDS             complement(<1..584)
                     /gene="yqeC"
                     /locus_tag="JQR05_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAY5326165.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAADEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHP"
BASE COUNT          179 a          169 c          149 g          155 t
ORIGIN      
        1 gggtggcgaa ccagcgcaac gagatcgctc agttgcaagg ttgcatcagg tgttaacccg
       61 gtaatatcag caaactgcga ccagcgatgg acattttctg tgctcacttt cgcgcccaaa
      121 atatgtcctc ccatcacagc aatcacgcag caactgcttt taggtatgca aggttcgtgc
      181 tcatcagccg cttttaacgg cattgcacgc gagccatctg cttcaatgag aattacgtca
      241 cactctggtc gttgcaccag tgcatcaatc gcttctggcg taaatccctg cacttttccc
      301 tggttcgctt tccagctgtg aaaacaaaat gaaatggggg atgtaagaga cgcatgagga
      361 agcatggcgg gatcacgaca gaaaaccacg ggccagtgag acgttggcat aaacatatgt
      421 gtcgtcgtag tgattaacac acgcctgccg ctagcctgga gcagttctgc cagccaaaaa
      481 agcaggctgg ttttcccccc agcaccaaca acagaaatca cagtgggacg tttctgcgca
      541 cctaaatcaa tgaataatgc cgatgggtca actatacttt tcacgagtct ttatgacctc
      601 tctgggataa attatcctca acttaatgca tcagaaagta acgcaattat ca
//
LOCUS       DADKXM010000356          650 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.359, whole genome shotgun sequence.
ACCESSION   DADKXM010000356 DADKXM010000000
VERSION     DADKXM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.359"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(54..242)
                     /locus_tag="JQR05_004253"
     CDS             complement(54..242)
                     /locus_tag="JQR05_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326166.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            358..650
                     /locus_tag="JQR05_004254"
                     /pseudo
     CDS             358..650
                     /locus_tag="JQR05_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          173 a          154 c          181 g          142 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca gccgtcaggc tgccgtggtt
      361 cagcgcctga tgggaatcct ctcacttaag gcagcgatta aggtcaagcg ataccgctct
      421 tacagaggag aggtagggca aaccgccccc aatgttctcc aaagagattt caaggctacg
      481 cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg gcgcaagctg
      541 tatttgcctc cagtaataga tctcttcaac aacgaagtta tttcttacag cctttcggaa
      601 agaccagtga tgaacatggt cgagaatatg ctcgatcagg cattcaaaaa
//
LOCUS       DADKXM010000357          645 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.360, whole genome shotgun sequence.
ACCESSION   DADKXM010000357 DADKXM010000000
VERSION     DADKXM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.360"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..298)
                     /locus_tag="JQR05_004255"
     CDS             complement(<1..298)
                     /locus_tag="JQR05_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326167.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEEL"
BASE COUNT          141 a          185 c          156 g          163 t
ORIGIN      
        1 tcagttcctc aggtaacagc cagtagagcc ggtaccggga tccgagcccc cgccagtcag
       61 ggtcactatt cccctccgtg tcggtaaaaa acaggtcccc ctgaaacggc aggtatccgt
      121 accggacaat ccggttatta ttcagacaca gcacgccata caggcacggt tcaccattaa
      181 caatgatatc gatatacatc cccgtagtac gctgattcag gcgaatggtg cagacctgag
      241 cgcccagtgt caccgtaaac tgctgggctt taacgggaga taaaacaatt tccagcatca
      301 ggtgatcccc ctgttcgtgg cgcttcgtct gtcagcgtct gacggttgtg tcaccgacgc
      361 cgtaactggc tgcgttttaa ccgatgcggc cccttttgct ttatcgttgt ccgtgggaga
      421 cgacttatcc gtacttccca ctgacacctc tcctgtattc attacggtct gaaataccgc
      481 agtaaccgtc agtaaggtcg gaccattatt acttcgcatt cggtagtcat atttcacaag
      541 gtcgcaggat gtccatgtct tgtccggcgt ctcaatatcg tagagtcctg ccgtggtacg
      601 catcctttca agcgtttcca gcacattcgt ccgcgaggtg gtgga
//
LOCUS       DADKXM010000358          636 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.361, whole genome shotgun sequence.
ACCESSION   DADKXM010000358 DADKXM010000000
VERSION     DADKXM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.361"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            148..354
                     /locus_tag="JQR05_004256"
     CDS             148..354
                     /locus_tag="JQR05_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY5326168.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          176 a          142 c          141 g          177 t
ORIGIN      
        1 tgtgaatgta aataaaccat caataagcaa aaataagtgg tcggatgagt agtaaaatta
       61 cacaaaaggc ggtactattt tcatcagata aacaaattca tttttccggt gctattcacc
      121 gggtagatac aaaaggggga gaaaagtatg gaactctaca gagaatatcc tgcatggctt
      181 atctttttac gccgaacata tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt
      241 ttctggaaag accgcgcccg cttttacagc tacctgcacc gcgtctggtc gaaaaccagc
      301 gataaaccgg tgtggatgga tcaggccgaa aaggcaaccg gtgattttta ctgattgcct
      361 gcgcgcttaa gccagcctcg tatcaggggg ctggtttagg tgttcaacat caacgtcttc
      421 catgataatt aacatggaag acgtttccat cgtattattc atttcatcac tgttttagca
      481 tcacgtttca atagtatcct ttcatagggc tacccaggat gcggtcacct tcattgtcgc
      541 tctaagcgac tagatcatca gaaatctcca gttagattgc atacttcctt acacactttc
      601 gtgaaacttc agggtaaggt gagaaagccg tcatcc
//
LOCUS       DADKXM010000359          626 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.362, whole genome shotgun sequence.
ACCESSION   DADKXM010000359 DADKXM010000000
VERSION     DADKXM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.362"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>626)
                     /locus_tag="JQR05_004257"
     CDS             complement(<1..>626)
                     /locus_tag="JQR05_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5326169.1"
                     /translation="DVKKRIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGT
                     LSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRANEKWVTDVTEFAVNGRKLYLSP
                     VIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNI
                     LKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAV"
BASE COUNT          162 a          153 c          119 g          192 t
ORIGIN      
        1 acagcatcct tcagttcgct tatattactg aactcatcaa gataaaaaca ctccgacttt
       61 aaggttccaa agaaacactc caccacagca ttatccagac aattgccttt tctggacatg
      121 ctttgtttaa taccatgttc tttaaggata ttttgatatc ttctcatacg atactgccat
      181 ccctggtcag agtgcagaac aggatgctcg tgaggattaa gctttttgaa tgcctgatcg
      241 agcatattct caaccatgtt catcactggt ctttccgaaa ggctgtaaga aataacttcg
      301 ttgttgaaga gatctattac tggagacaaa tacagcttgc gcccattgac tgcaaattca
      361 gtaacatcgg taacccactt ctcgtttgcc cgcgtagcct tgaaatctct ttggagaaca
      421 ttaggggcgg tttgccctac ctctcctctg taagagcggt atcgcttgac cttaatcgct
      481 gctttaagtg agagggttcc catcaggcgc tgaacagctt tatggttaat ctgtttccct
      541 tctcgatgaa gagacagcgt taccctacgg tatccgtatc ggcctctatt ctcgtgataa
      601 atctcaccaa tacgcttttt aacgtc
//
LOCUS       DADKXM010000360          622 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.363, whole genome shotgun sequence.
ACCESSION   DADKXM010000360 DADKXM010000000
VERSION     DADKXM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.363"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..109)
                     /locus_tag="JQR05_004258"
     CDS             complement(<1..109)
                     /locus_tag="JQR05_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY5326170.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDN"
     gene            305..592
                     /locus_tag="JQR05_004259"
     CDS             305..592
                     /locus_tag="JQR05_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HAY5326171.1"
                     /translation="MAQVYFDTLKFVETLEAAGMPAAQARAISAAVKDSHEAAEVATK
                     RDLNDVRTELKNDIAALDAKIDKLSLQLTVRFGGMLVVAVGALTAIIKLPL"
BASE COUNT          171 a          172 c          151 g          128 t
ORIGIN      
        1 aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg ttggcgtaac
       61 cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata aatcgcgaat
      121 gtgactatgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct ccgtaaacat
      181 ccggggtgct aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccggatta
      241 acgttgtcgt caacagccac tacaggctaa actaacaata ttccccaaca acgaaggatt
      301 taacatggct caggtctatt ttgatacact gaagttcgtc gaaaccctgg aagccgcagg
      361 aatgccggca gctcaggcgc gcgctatatc tgccgcagtg aaagattctc acgaagccgc
      421 agaagtcgcc acaaagcgcg acctgaatga tgtcagaact gaactaaaaa acgacattgc
      481 agcccttgat gcaaaaatag acaaactcag cctgcaactg accgtcagat ttggtggcat
      541 gctcgtcgtt gctgttggcg cactgacagc catcatcaaa ttacctcttt aaattaccag
      601 cccgttcggg gctggtttac ct
//
LOCUS       DADKXM010000361          608 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.364, whole genome shotgun sequence.
ACCESSION   DADKXM010000361 DADKXM010000000
VERSION     DADKXM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.364"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..608
                     /locus_tag="JQR05_004260"
                     /pseudo
     CDS             1..608
                     /locus_tag="JQR05_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          160 a          148 c          170 g          130 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccggtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccggggcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aacgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgca
//
LOCUS       DADKXM010000362          587 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.365, whole genome shotgun sequence.
ACCESSION   DADKXM010000362 DADKXM010000000
VERSION     DADKXM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.365"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JQR05_004261"
     CDS             complement(<1..491)
                     /locus_tag="JQR05_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5326172.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIMPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINA"
BASE COUNT          139 a          179 c          132 g          137 t
ORIGIN      
        1 gcattaatca caccctcagg cgcggatatc tccactttcc agggagatac aatccgtatc
       61 tggttgtcat caaataccac aaactggacg ggatcaccgt tgagcacgcc acccagccag
      121 atggcatcag cataattatg ggtacgcttt gatcccggca tcgcagcctg acgcgtggct
      181 tttaccgcac tgatatcccg atcgcagatg gcaagaaaac cgatatcccc cacatgtggc
      241 ggcataatca ccgcattacc gcccccctgt agtcgccata cgggaatgtt ataaatcacc
      301 tcatgttcaa ccggggaacc gtctgctgca acgcccatta ccatcggtcg ggcctcaata
      361 aatccctctt ccacggctac cacccgtccc agagtgataa acgcatgacg tccgagaaac
      421 tgccgcagca taaagtcctg tgcattgatt tcgctgttca cgtctgtcgg gttactgagt
      481 ggttgtgcca ttttcttcag ccctcatcat ggtacagttg gaactccacg gaccgcccac
      541 ggttcgcgaa gtaatggtgt gtatcacccc tgtgatcgta taatcgc
//
LOCUS       DADKXM010000363          580 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.366, whole genome shotgun sequence.
ACCESSION   DADKXM010000363 DADKXM010000000
VERSION     DADKXM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.366"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..128
                     /locus_tag="JQR05_004262"
     CDS             <1..128
                     /locus_tag="JQR05_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5326173.1"
                     /translation="GRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            125..>580
                     /locus_tag="JQR05_004263"
     CDS             125..>580
                     /locus_tag="JQR05_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5326174.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEM"
BASE COUNT          170 a          151 c          154 g          105 t
ORIGIN      
        1 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggatcgcg
       61 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
      121 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
      181 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
      241 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
      301 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
      361 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
      421 caccagtccc ccgtcagaaa acccgatcgc cagtaagcct gaatccccgg gacgaaaatc
      481 ttcacgaaaa ccgctgccgg cagaacttcc ccgggagaca catcgccttc tgcctgctga
      541 aaccagttgc ccggcctgtg gaggtgttct gaaagaaatg
//
LOCUS       DADKXM010000364          579 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.367, whole genome shotgun sequence.
ACCESSION   DADKXM010000364 DADKXM010000000
VERSION     DADKXM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.367"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            289..570
                     /locus_tag="JQR05_004264"
     CDS             289..570
                     /locus_tag="JQR05_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326175.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          156 a          135 c          134 g          154 t
ORIGIN      
        1 aaaataattc attgatattc aaaattaatt ttctacagga atacagaggc ggcgggaaga
       61 tatgccctta atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta
      121 atatggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt
      181 gtttcatttt tgtgttctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa
      241 caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc
      301 accactggcg tggttcttct tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc
      361 agcacactca acatccgttt tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc
      421 ggtctgggcg ttgtttccgc ctgttggttc gcaatggggt tgcttggcat cacaatggcg
      481 gacatcaccg ctatctggca caacatcgag tcggtgatga tagaagagat gaatcagaca
      541 ccgccacaat ggccaatgat tttgacctga tacgtagaa
//
LOCUS       DADKXM010000365          579 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.368, whole genome shotgun sequence.
ACCESSION   DADKXM010000365 DADKXM010000000
VERSION     DADKXM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.368"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..304
                     /locus_tag="JQR05_004265"
     CDS             <1..304
                     /locus_tag="JQR05_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5326176.1"
                     /translation="PSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTK
                     GMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          168 a          125 c          118 g          168 t
ORIGIN      
        1 tccctcagcg tggattgccg ctcatgcagg agcagataat cccttcgcca tcccattaag
       61 cgccgttgtc ggtattccgc tgtatatccg ggctgaggcg gttatccctc tggcatctgt
      121 tctgatgaca aaaggaatgg gtctgggagc attaatggca ttaatcatcg gcagtgccgg
      181 cgcaagcctg acggaagtga tattacttaa atcaatgttc agaatgccga tgatagctgc
      241 attcctgacg gttatattag gtatggctat cttgatgggc tatttgactc aattgctatt
      301 ttaaatagat gccagcagta aactataaga tgcatagtta gtgccatatc cttctaagta
      361 gaaaatcata gacaggataa tccttaaaaa tcacaaatga tttaactaat gcattgagaa
      421 tgatgacggc tcggtcaaca agtcatcaac agacaataac cacgtaatca tgcttctctg
      481 acattttatc ggagaagcaa tgactctttg tctcagtacc ctcctccttt ttaaaaggca
      541 gatttcagtc gctaatacaa cacattttta aatacactt
//
LOCUS       DADKXM010000366          574 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.369, whole genome shotgun sequence.
ACCESSION   DADKXM010000366 DADKXM010000000
VERSION     DADKXM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.369"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..236)
                     /locus_tag="JQR05_004266"
     CDS             complement(<1..236)
                     /locus_tag="JQR05_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAY5326177.1"
                     /translation="MKIVLLGTKGGPSLLKADRLPMATALIIGNKTWLIDAGYGASLQ
                     LVKNNIPLRQIDNILLTHLHSDHILDYPSVLMNA"
     gene            complement(344..>574)
                     /locus_tag="JQR05_004267"
     CDS             complement(344..>574)
                     /locus_tag="JQR05_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5326178.1"
                     /translation="LGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSIN
                     QIALDLGYKDPAYFARFFNRMMGCSPTQFRGR"
BASE COUNT          157 a          120 c          140 g          157 t
ORIGIN      
        1 catgcattca ttaagactga tggatagtcc agaatatgat cactgtgtaa atgggtgagt
       61 aaaatattat caatctggcg cagtggaata ttatttttta ccagttgtaa gctggccccg
      121 taaccagcat caattaacca ggttttattg ccaataataa gtgccgttgc catcggtaaa
      181 cggtcagctt ttaataaaga agggccacct ttcgttccaa gtagtacaat tttcatttat
      241 tgcggttcgg aggcgtaggt gaatgccact ggcgcaatca ggcacgccac aaatagtgag
      301 cgtaaagata ttttcatgtc gagtctctca ggttgataaa tggtcagcga ccacggaatt
      361 gtgttggtga gcaacccatc attcggttga aaaaacgggc gaaataggcg ggatctttgt
      421 agccaagatc aagggctatc tggttgatgc tttgctcgct atagagcaga agccgtctgg
      481 cttcccggct cactcgttcg aaaatcaatc tcttcggcga ttgattggca aaacgtcggc
      541 acagctcggt aagccgtgat tcactcatac cgag
//
LOCUS       DADKXM010000367          573 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.370, whole genome shotgun sequence.
ACCESSION   DADKXM010000367 DADKXM010000000
VERSION     DADKXM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.370"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..365)
                     /locus_tag="JQR05_004268"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="JQR05_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            288..539
                     /locus_tag="JQR05_004269"
     CDS             288..539
                     /locus_tag="JQR05_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326179.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          156 a          132 c          142 g          143 t
ORIGIN      
        1 gccagtgttg ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa
       61 gggcaacatc agcgtgagcg actggcgcag atgttatccg ccggggcgag agctttttaa
      121 tgtccgtatg acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc
      181 gccgttatgt gccatttcaa cgatctgctc cttaacgccg agtttctggc ttcataagag
      241 taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg ccgggaacaa
      301 ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac agtaacggta
      361 gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt gaagacatta
      421 ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg gtggcagttg
      481 gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca ataatctaaa
      541 tagctgcgct taataccgct acacttttgc cag
//
LOCUS       DADKXM010000368          553 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.371, whole genome shotgun sequence.
ACCESSION   DADKXM010000368 DADKXM010000000
VERSION     DADKXM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.371"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..498)
                     /gene="acrE"
                     /locus_tag="JQR05_004270"
     CDS             complement(<1..498)
                     /gene="acrE"
                     /locus_tag="JQR05_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAY5326180.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESA"
BASE COUNT          123 a          142 c          145 g          143 t
ORIGIN      
        1 agcgctttcg actgtggctt ttgcggcaat cacggcggca tcggcctggc gagcatcagc
       61 aatggcctgg tcgtactcct gctggctgat gtatttcgta cccacgagcg gaacgtaacg
      121 ttttaccgtc aaatgcgcga tggcggcggc ggcttcactt ttcgccagtt cgcctttcgc
      181 gctgtcataa tttgcctgat aggtcgcggg atcgatctgg tatagggact ggcctgcttg
      241 tacatcgctg ccttcagtga aattacgatt cagtacgatc ccgctaacct gtgggcgaac
      301 ttcggctata cggtaagcat tggtgcggcc tggtaattca gtcttaactt ctaacggggc
      361 cgttttcaca atatgaacgg taacctgcgg ttcaccgacg tgagctttct cttctccctt
      421 atcgttacaa ccggcgatta aagccgcgga gatcagaata aaggagggca ggaggaaaaa
      481 cctggcatgt ttcgtcatta ctattcctca aaaaaccaaa agcgcgttat ttacccagaa
      541 aggcaacgcg tta
//
LOCUS       DADKXM010000369          550 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.372, whole genome shotgun sequence.
ACCESSION   DADKXM010000369 DADKXM010000000
VERSION     DADKXM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.372"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..208
                     /locus_tag="JQR05_004271"
     CDS             <1..208
                     /locus_tag="JQR05_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5326181.1"
                     /translation="VYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHM
                     DLYDGKAYVAEAISVLAPFFEETL"
     gene            208..>550
                     /locus_tag="JQR05_004272"
     CDS             208..>550
                     /locus_tag="JQR05_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5326182.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          145 a          113 c          148 g          144 t
ORIGIN      
        1 agtgtacctg actcagccga tgcagattgt ggcgggcagc caggcaggga gcaaatggat
       61 gagtgacgac ctgtacgatc gggcatcaag ccaggataaa cgctatcaca ttgttgaagg
      121 cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa tttcagtatt
      181 agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa tagtgatatg
      241 gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc aaaacagtat
      301 ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtc tggcaatatc
      361 tatggcaagg cattggctga aaaaggctat gtcgttctcg cttatgacgc cagtttccag
      421 ggagaatctg gtggggagcc tcgttggata gaagatccga cacagcgcgt agaggatatt
      481 agccgtgtta tcgattatgc agtaactctg ccatatgttg atgctgaacg cattggcgtg
      541 ctgggtgtgt
//
LOCUS       DADKXM010000370          547 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.373, whole genome shotgun sequence.
ACCESSION   DADKXM010000370 DADKXM010000000
VERSION     DADKXM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.373"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>547
                     /locus_tag="JQR05_004273"
     CDS             <1..>547
                     /locus_tag="JQR05_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5326183.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLR"
BASE COUNT          178 a          116 c          120 g          133 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgaga
//
LOCUS       DADKXM010000371          541 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.374, whole genome shotgun sequence.
ACCESSION   DADKXM010000371 DADKXM010000000
VERSION     DADKXM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.374"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..90
                     /gene="yjdI"
                     /locus_tag="JQR05_004274"
     CDS             <1..90
                     /gene="yjdI"
                     /locus_tag="JQR05_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY5326184.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            102..374
                     /locus_tag="JQR05_004275"
     CDS             102..374
                     /locus_tag="JQR05_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5326185.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          173 a           96 c          144 g          128 t
ORIGIN      
        1 tgggtcatgc cggatgaagt cgacgtcgcc actgtggtta aagtgattga tacgtgcccg
       61 agcggcgcgc tgaaataccg tcataaataa gcgagggtaa aatggaaata tgcgaaggcc
      121 acaataattt ttacattaat gacgaacaag gcaagcaaat cgctgaaatt gtctttgtgc
      181 cgaccggaga gaatttagcg attatcgaac ataccgatgt cgatgaaagc ctgaaagggc
      241 aagggattgg taaacagctg gttgcgaaag tcgtggaaaa aatgcgtcgg gaaaaacgaa
      301 aaattatccc attatgtcca tttgcgaaac acgaatttga taaaacgcgg gagtatgatg
      361 atattcgcag ttgatgggag agtacagagt cacgatattt ttcttgctct ccgcggtgtg
      421 atgcaggaga gcatctgaaa ggtaggggga tgcacaaaga atgggcagag agcgcgtttt
      481 tttgtcccaa gtcatcccct ttactgagca aaaaaaagaa tatctcctat atgagaatca
      541 t
//
LOCUS       DADKXM010000372          531 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.375, whole genome shotgun sequence.
ACCESSION   DADKXM010000372 DADKXM010000000
VERSION     DADKXM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.375"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..333)
                     /locus_tag="JQR05_004276"
     CDS             complement(<1..333)
                     /locus_tag="JQR05_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5326186.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMG"
BASE COUNT          156 a          129 c          104 g          142 t
ORIGIN      
        1 ccccatctcg acaatttcta ccggaattcc tatgcgggca tgcacttccg cgacggtatg
       61 ctggatttgt attagccttt cgacatcgtc aacctgggca gaaagcacgt taataatcca
      121 gcgttccatc gcacttttca actgccgctc aacttgttca ttactcaaga attcttcggc
      181 atgcgggtcg atgcggacaa ttcgataaaa ctcgatactc agataatgag catgcgcaag
      241 ggcaatttct gcggctttag cacgaatgag aggatctgcc tgttcgacaa gtccggtcca
      301 ctcatctttc attcttttaa aatacatctc cataattcac acccttataa ggctgggaaa
      361 tcagacggaa taaaaacgaa acgcaacgtg cgagatcgac taactgcacc atattctcct
      421 gaaatatgaa gatatactga aaagaaataa gcgacttaag acagtttcaa tctacgctac
      481 tgttcttcag aagagtatag cccatcgtaa ttatttttcg gtgacagcga a
//
LOCUS       DADKXM010000373          509 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.376, whole genome shotgun sequence.
ACCESSION   DADKXM010000373 DADKXM010000000
VERSION     DADKXM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.376"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JQR05_004277"
     CDS             <1..489
                     /locus_tag="JQR05_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5326187.1"
                     /translation="KKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     LLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          162 a          104 c          112 g          131 t
ORIGIN      
        1 aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctacaaag cgacattacc aatcattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta tgatgaaaaa
      301 aaacatctcc tgactattga aattcggcca ccaatggatg cttgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gccacatccg
      421 gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaagtatt
//
LOCUS       DADKXM010000374          507 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.377, whole genome shotgun sequence.
ACCESSION   DADKXM010000374 DADKXM010000000
VERSION     DADKXM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.377"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            5..507
                     /locus_tag="JQR05_004278"
                     /pseudo
     CDS             5..507
                     /locus_tag="JQR05_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          113 c          126 g          110 t
ORIGIN      
        1 aataatgagc agaaaaaccc aacgttactc taaagagttc aaagccgaag ctgtcagaac
       61 ggttcttgaa aatcaacttt cgatcagtga aggcgcttcc cgattatctc ttcctgaagg
      121 cactttagga caatgggtta ccgccgccag aaaagggctc ggtactcctg gttcccgcac
      181 ggtggctgaa ctggaatctg aaattctgca actgcgtaag gcgttaaatg aagctcgcct
      241 tgagcgagat atattaaaaa aagcaacagc gtattttgca caggagtcgc tgaaaaatac
      301 gcgttaatcg aacaatggcg acaacaattt cccattgaag cgatgtgtca ggtatttggt
      361 gtatccagga gcggttatta caactgggta cagcatgaac cctcagacag aaaacaaagt
      421 gatgagcggc taaaactgga gattaaggtg gcacatatcc gcactcgcga aacatatgga
      481 acccggcggc tccagacgga gctggca
//
LOCUS       DADKXM010000375          487 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.378, whole genome shotgun sequence.
ACCESSION   DADKXM010000375 DADKXM010000000
VERSION     DADKXM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.378"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..139
                     /locus_tag="JQR05_004279"
     CDS             <1..139
                     /locus_tag="JQR05_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5326188.1"
                     /translation="SDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPVSPEIS
                     S"
     gene            163..>487
                     /locus_tag="JQR05_004280"
     CDS             163..>487
                     /locus_tag="JQR05_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326189.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEE"
BASE COUNT          137 a          108 c          123 g          119 t
ORIGIN      
        1 cagcgacgaa gataaagaaa agctgaaagc ctgtcgtatt tacgcgaaat cgctgcaggc
       61 gatggatttc agcaccatca ctgataaatc ctcatacaac gccattgaat ggcccgtctc
      121 tccggaaatt tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt agtgatatca
      181 ggtgcgctga ctgatggcgc aggtatcccc atgtccggat accatattat tctgaaatcc
      241 cgggtaaaca ccccggaagt ggtgatgcac actgttgctg atgtgatgac aggaaacgat
      301 ggtgaatact gtttccatgc gcggactgga aaatatggtg tgtatctgaa acaggactgg
      361 cacaacgagt acaacgttgg cgacattgct gtatatgagg actcaaagcc cggcacgctg
      421 aatgactttc tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt
      481 gaggaaa
//
LOCUS       DADKXM010000376          474 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.379, whole genome shotgun sequence.
ACCESSION   DADKXM010000376 DADKXM010000000
VERSION     DADKXM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.379"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            64..270
                     /locus_tag="JQR05_004281"
     CDS             64..270
                     /locus_tag="JQR05_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326190.1"
                     /translation="MTETTSQQDDYEGICLEPDSFAVNVYHLLHATQVLHTSSNNETK
                     TLGSEILNFACEYAKAAVEKELAQ"
BASE COUNT          160 a           94 c           93 g          127 t
ORIGIN      
        1 aggagtgtcc gttaaactca ggctacctca gtcaaaaaca ggctcgcatg tacttcagga
       61 gcaatgacag aaacaacctc acagcaagat gattatgaag gcatttgtct ggagcctgat
      121 tcatttgcgg taaatgttta tcatttattg catgcaacac aggtgttaca tacgtccagt
      181 aataacgaaa caaaaacact tggcagtgaa attctgaatt ttgcatgtga atatgcaaaa
      241 gccgctgttg aaaaagaatt agcgcaataa caacaaatat gccctgaacg tttattgcgg
      301 ttttatcgcc ggggattgtt acaaccttaa tccacaggag gctttattat gacttttata
      361 aagaatatgg catcacacaa gaccgccagc cttattgcac aatacggtga aaattacatg
      421 catattgcct gcttatttct gcgtaaagca tacgggaaat aacaaatgac acat
//
LOCUS       DADKXM010000377          468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.380, whole genome shotgun sequence.
ACCESSION   DADKXM010000377 DADKXM010000000
VERSION     DADKXM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.380"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>468
                     /locus_tag="JQR05_004282"
     CDS             <1..>468
                     /locus_tag="JQR05_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326191.1"
                     /translation="MVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYN
                     VLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSP"
BASE COUNT          121 a          114 c          148 g           85 t
ORIGIN      
        1 catggtgcgc tgggtatcag aaatggcaga caaactccgt cctctgtata tagcgctgaa
       61 tgactatgtt ctggaggcag gaaaggtgca cgcagatgac actccggtga aagtactggc
      121 cccggggaac ggaaagacga aaacgggtcg tctgtgggta tacgtcaggg atgatcgtaa
      181 tgcgggttca tccctgccgg cagccgtctg gttcgcgtat tcggcagatc gcaaaggaga
      241 acatccgcag ctccacctgg caaagtatca gggcgtactg caggctgatg cctatgcagg
      301 ttataacgta ctgtacgaaa cgggccgggt gaaggaagcc gggtgcctgg cccacgcccg
      361 ccgaaaaatc catgacgagg atgtgcgccg tccgacagaa atgactcagg aagcgctcag
      421 acggatagca gagttatacg acatagaagc ggagatacgt ggcagtcc
//
LOCUS       DADKXM010000378          455 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.381, whole genome shotgun sequence.
ACCESSION   DADKXM010000378 DADKXM010000000
VERSION     DADKXM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.381"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            29..455
                     /locus_tag="JQR05_004283"
                     /pseudo
     CDS             29..455
                     /locus_tag="JQR05_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a          101 c          115 g           95 t
ORIGIN      
        1 aatagaatgg catcagaagg agccaataat gagcagaaaa aaccaacgtt actctacgga
       61 gtttaaagcc gaagccgtca aaacggttcc tgaaaatcag ctttcgatca gtgaaggcgc
      121 ttcccgatta tctgttcctg aaggcacttt aggacaatgg gttaccgccg caagaaaagg
      181 gctcaataca ccaggctccc gcacagtggc tgagctggaa tctgaagtta tgcaactgcg
      241 taaggcttta aatgaagcac gacttgagcg agatatatta aaaaaagcaa cagcgtattt
      301 tgcccaggag tcgctgaaaa atacgcgtta gtcgaacaat ggcgacaaca attccctgtt
      361 gaagcgatgt gtcgggtacc tggtgtatcc aggagcggtt attacgaccg ggtacagcat
      421 gccccctcag acagaaaaca aagtgatgag cggct
//
LOCUS       DADKXM010000379          452 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.382, whole genome shotgun sequence.
ACCESSION   DADKXM010000379 DADKXM010000000
VERSION     DADKXM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.382"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..339
                     /locus_tag="JQR05_004284"
     CDS             <1..339
                     /locus_tag="JQR05_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326192.1"
                     /translation="DDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRTVGV
                     QDVRYGHEIYYSTGSNTVSWRFRAPAGHGLSGISISDTGKNSADNVNGVYYRPLQKLI
                     NGTWYNVASV"
BASE COUNT          141 a           92 c          109 g          110 t
ORIGIN      
        1 gatgattcag gctggcattt tttcagccag aggaatacag ataacagcat cacgttcgcc
       61 gtaaacggac aggtaattcc gttaaattac ggaaacttcg atgcccgcta taaatatcga
      121 acagtggggg tacaggatgt acggtatggc catgaaatat attacagcac cggcagtaac
      181 accgtttcgt ggagatttcg cgcacctgcc ggacacggat tgtcagggat atcgatatcg
      241 gatactggta aaaattcagc ggataacgtc aacggcgtgt attaccgccc gcttcagaaa
      301 ctgattaatg gcacctggta taacgtggcg agtgtttaac aatgttgcat ttaaaaaata
      361 ttactgcagg taatccgaaa accgtggaac aatatcagct gacaaaacaa tatgatgtca
      421 cctggctttt ttcggaagac ggaaaaaact gg
//
LOCUS       DADKXM010000380          448 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.383, whole genome shotgun sequence.
ACCESSION   DADKXM010000380 DADKXM010000000
VERSION     DADKXM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.383"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            161..>448
                     /locus_tag="JQR05_004285"
     CDS             161..>448
                     /locus_tag="JQR05_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5326193.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIV"
BASE COUNT          138 a           81 c           91 g          138 t
ORIGIN      
        1 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
       61 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
      121 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      181 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      421 gtttgccaca gcattgcaat ccatagtg
//
LOCUS       DADKXM010000381          438 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.384, whole genome shotgun sequence.
ACCESSION   DADKXM010000381 DADKXM010000000
VERSION     DADKXM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.384"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>438)
                     /locus_tag="JQR05_004286"
     CDS             complement(<1..>438)
                     /locus_tag="JQR05_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY5326194.1"
                     /translation="RAGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPR
                     LTKSEQTAYGNALGFSYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVE
                     LDQTWSGIGMTISTALMPYALKLSHWLETLGDWMQQHPEEVNR"
BASE COUNT          100 a          131 c          109 g           98 t
ORIGIN      
        1 acctgttcac ttcctccgga tgttgctgca tccagtcacc gagtgtctcc agccagtggc
       61 tgagcttcag cgcgtacggc atcaacgcgg tgctaatcgt catcccgatg ccggaccagg
      121 tctgatcgag ctcaacaagc tcttttcgta gcttacgggc cagcgctgta cgccccggcg
      181 taacctgcga acgggaagta aattcgtcca catctttcag cgcatgaccg gagccaagaa
      241 actgctgtcc ggcataactg aagcccagcg cgttgccgta ggcagtttgt tcggactttg
      301 tcagtcgcgg aaaaataacc gccagcttgc gcatgatggc ttcagtgctg tcagtattta
      361 aatcaacact aatgcccgcc ctggctgcaa cctgaaacaa atcctgtaac acaggatcaa
      421 aggaccttcc ggctctga
//
LOCUS       DADKXM010000382          434 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.385, whole genome shotgun sequence.
ACCESSION   DADKXM010000382 DADKXM010000000
VERSION     DADKXM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.385"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(1..105)
                     /locus_tag="JQR05_004287"
                     /pseudo
     CDS             complement(1..105)
                     /locus_tag="JQR05_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672075.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            236..>434
                     /locus_tag="JQR05_004288"
     CDS             236..>434
                     /locus_tag="JQR05_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5326195.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVIIRPKLACSRCDVIV"
BASE COUNT          112 a          124 c          107 g           91 t
ORIGIN      
        1 atccccgcca gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcta
       61 cagagctgaa aacccacaag gccatgcccc gcatggccgg gtaaagcgga gccggcggcg
      121 acttcctttg tgctctgtcc gagaccggca ggaaaaaggc ggggataatg agatggtcac
      181 ccccacccta acagcagtac gctgttaagg tgaatccctt aacggagaga ccatcataca
      241 gaacatcaca cttattggta tcgatctcgg aaagcattcc ttccacattc attgtcaaga
      301 caagtctggt aaggctcttc tgcgtaaaaa atttacccgt actaaactga tgcagtttct
      361 ggcaacatgt ccgccctcag ttgttgtaat catacgtccc aaactggcct gtagccggtg
      421 tgatgtcatc gttc
//
LOCUS       DADKXM010000383          425 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.386, whole genome shotgun sequence.
ACCESSION   DADKXM010000383 DADKXM010000000
VERSION     DADKXM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.386"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(311..>425)
                     /locus_tag="JQR05_004289"
     CDS             complement(311..>425)
                     /locus_tag="JQR05_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5326196.1"
                     /translation="YLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          151 a           64 c           67 g          143 t
ORIGIN      
        1 atctattttc attgatttga ttcaatataa caatagatta cagaggatta acttccagtt
       61 acttaaaaaa taaagaaaaa agtgtggaaa caggagaatt taagtgatgt gtaaacttat
      121 gaatgatttt gtttatttat tttgaagaac ataaaccatt agattttgta tgggtatggt
      181 taacttaatg tttattatta taattaagaa tcacacttac aataacatca attaataaat
      241 ggttaatcat ttttgatata tcgtaagaat aatgtagttt ttaacaccat ccctggtatc
      301 tcaactatct ctataaaaca gcatgacgct gtcgtcctct ggctctatcc cagatgccgt
      361 aaaaacgccc tgcattgctg gcggtatacc agacagtatg acgaatattg cgatgcccaa
      421 gataa
//
LOCUS       DADKXM010000384          424 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.387, whole genome shotgun sequence.
ACCESSION   DADKXM010000384 DADKXM010000000
VERSION     DADKXM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.387"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..205)
                     /locus_tag="JQR05_004290"
     CDS             complement(<1..205)
                     /locus_tag="JQR05_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326197.1"
                     /translation="MRLWADGRLIFEGDITDAYADYNQAPDIPLILTGQIHFNLRNQP
                     AADFSAKGDVAVADIIRALASTVG"
BASE COUNT          103 a          127 c           94 g          100 t
ORIGIN      
        1 gcccgacagt tgacgccagc gcacggataa tatctgcaac cgcaacatcg ccctttgcgc
       61 tgaaatctgc cgccggctga ttacgcagat taaaatgaat ctgtcctgtc agaatgagcg
      121 gaatatccgg cgcctggttg tagtcggcgt atgcatcagt aatatcacct tcaaaaataa
      181 gccgcccgtc ggcccacagc cgcattttat ttgcggtact ctgctcctgt cgccacacac
      241 cgcgccagct taaattcgtc atatgcgccg gtgacattcc ccagatatac agcgtgattt
      301 gtgtaccagc cacccctcca tatacggcaa tactggcaaa acagcttgcg tcggtaatgg
      361 tcagcatatt gccctggcca ttaccaaacg ttcgtccgtc tgaaagagtg aactccaccg
      421 ttat
//
LOCUS       DADKXM010000385          420 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.388, whole genome shotgun sequence.
ACCESSION   DADKXM010000385 DADKXM010000000
VERSION     DADKXM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.388"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>420
                     /locus_tag="JQR05_004291"
     CDS             <1..>420
                     /locus_tag="JQR05_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY5326198.1"
                     /translation="IATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHV
                     AIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSR
                     LTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLL"
BASE COUNT           93 a          107 c          134 g           86 t
ORIGIN      
        1 atcgccacaa tggcaacccg gagaggctac cagatgggac gctggcttgc tggcaggctc
       61 atgaaagagc tggggctggt cagctgtcag cagccgactc accggtataa acgtggtggt
      121 catgaacatg ttgctatccc taactacctt gaaaggcagt tcgccgtgac cgagccaaat
      181 caggtgtggt gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc
      241 gttgttctcg acctgttcgc aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac
      301 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
      361 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
//
LOCUS       DADKXM010000386          413 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.389, whole genome shotgun sequence.
ACCESSION   DADKXM010000386 DADKXM010000000
VERSION     DADKXM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.389"
                     /strain="IB1896"
                     /db_xref="taxon:623"
BASE COUNT          109 a          115 c           98 g           91 t
ORIGIN      
        1 gtaatacagc atgtaccaga cccgacacgg catttctgac cagggaaaaa gtcaacgatg
       61 agtagtgata tcagcgcaaa tgacgtcctg tggctccttt gcggtgacca cattcacgtc
      121 tccggcgctg ccagaggata tttatttcaa ggaaacgtag cattatggct agctttaaag
      181 acatctgccc attcgccccc gccattcccc catcttccgt taacccaggc gtacgacgcc
      241 cgccagggcg gctcttcctg tcggtggagc tcaagaatca aattttgcag aacatgaagt
      301 atctcagccc gcaaaaattc agctggggtg atgcccccta gcagatcatt gacctcagta
      361 tcgcgggcaa agtgaacata caggtggaca acaataccat catcacgttg ggg
//
LOCUS       DADKXM010000387          412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.390, whole genome shotgun sequence.
ACCESSION   DADKXM010000387 DADKXM010000000
VERSION     DADKXM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.390"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>412)
                     /locus_tag="JQR05_004292"
     CDS             complement(<1..>412)
                     /locus_tag="JQR05_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY5326199.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEA"
BASE COUNT          117 a          101 c           72 g          122 t
ORIGIN      
        1 agcttcagct agttgcccct taagctcttc agccataatc tgacccttac cagccatctg
       61 tgataatgct gtcagtgctc gcttaatctc ttcttcactt cgccccatca gacgagaata
      121 gccaatcata tttctaaaaa gttcttcaga accttgaatg cctagagact ctctagctga
      181 agcaccgaaa ccagcaaact gtgattgcag ttcagcgata tctaaacccg ttgtttgagc
      241 taatgacttt gtgaaatctt ttacttgatt tgcatcttta gaaaatacga aatcaattga
      301 acgttcagca cttgcacgtt gaaggccaac ttccattact cgctgataag cggatagtac
      361 agcctgaaag ctgataagta cacctgctgt tttaactgct gctgaactta aa
//
LOCUS       DADKXM010000388          409 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.391, whole genome shotgun sequence.
ACCESSION   DADKXM010000388 DADKXM010000000
VERSION     DADKXM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.391"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(195..>409)
                     /locus_tag="JQR05_004293"
     CDS             complement(195..>409)
                     /locus_tag="JQR05_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HAY5326200.1"
                     /translation="ETATLTINGRISPPTCSMAMVNSQPQQHCGQLTYNVDTRHQVSS
                     PVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          138 a           71 c           67 g          133 t
ORIGIN      
        1 ttgttttatt gatttaaaac aaattaacca atgcaatata aatacaacaa atacttaaga
       61 gtattaaata tttatcacat ctattaatta atgtagattt atgtaaatgg tattgttgat
      121 atcaataaaa aagacgaaat catttatata aatgatttcg tctttctaaa tgcaattaaa
      181 atcaggttgc caaattaatc gtagcgattt aacacgattc gacgtttgct atcgctacct
      241 gcaacaacca cttccgtcgt aacacctttg acgggcgaag aaacctgatg acgggtgtca
      301 acattatacg ttaactgacc acaatgctgt tgtggttgac tattaaccat agccatactg
      361 catgttgggg gactgatcct gccattaatc gttaatgtgg cagtctctg
//
LOCUS       DADKXM010000389          405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.392, whole genome shotgun sequence.
ACCESSION   DADKXM010000389 DADKXM010000000
VERSION     DADKXM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.392"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..392)
                     /locus_tag="JQR05_004294"
     CDS             complement(<1..392)
                     /locus_tag="JQR05_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5326201.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKRCLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSL"
BASE COUNT          107 a           76 c          115 g          107 t
ORIGIN      
        1 agtgaaacat caagagcgac caggtgaggc ggaagagccg ggagcctgct cagtctgtta
       61 tcgctggctt taagtacagt taaggacgga gggagttctg gcagagagcg tagctcattg
      121 ctagagatgt taagttcttg tatgtgcggg ggcaggtatg ggagagagcg taatctgagt
      181 aaacttaaat tgagggctgg ctcttgaaaa gccagacatc ttttcagtaa tcgaactgcc
      241 tgtgttcggt cttctgttgc agcaccttcc ttggcccagt tatcccagat gcggtgataa
      301 tccgtgatat tttgctcttc tacggataaa cgggatatac atcctaaacc acaaaaggag
      361 cggaaaaaag aaggattgtg ggcaggtttc atttattatt gatcc
//
LOCUS       DADKXM010000390          404 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.393, whole genome shotgun sequence.
ACCESSION   DADKXM010000390 DADKXM010000000
VERSION     DADKXM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.393"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>404
                     /locus_tag="JQR05_004295"
     CDS             <1..>404
                     /locus_tag="JQR05_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5326202.1"
                     /translation="FLDAGGLRDIEVEAVTKMLACGTRILGVKEYNCDKPDCPHVRYV
                     TNSCGSRACPSCGKKATDLWIETQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDV
                     CRLAVENLLYAARKRGQEPGIFCAIHTYGRRL"
BASE COUNT           84 a          117 c          123 g           80 t
ORIGIN      
        1 ttcctggatg cgggcggtct gcgcgatatc gaggttgagg ctgtcacaaa aatgctggcc
       61 tgcggcacgc ggatactggg cgtgaaggaa tacaactgcg ataaacctga ctgcccccac
      121 gtcagatacg tcactaactc atgcggcagc cgtgcctgcc cgtcctgcgg aaaaaaggcc
      181 acagacctgt ggatagagac acagctgaat cgtcttcctg actgcgactg ggtacacctg
      241 gtcttcaccc tgccggacac gctgtggccg gtgttcgaaa gcaaccggtg gctgctgaat
      301 gacgtgtgcc gtctggcggt ggagaatctg ctgtatgccg cccggaaacg ggggcaggaa
      361 cccggtatct tctgcgccat ccacacgtat ggccgtcgtc tcaa
//
LOCUS       DADKXM010000391          401 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.394, whole genome shotgun sequence.
ACCESSION   DADKXM010000391 DADKXM010000000
VERSION     DADKXM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.394"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(319..393)
                     /locus_tag="JQR05_004296"
     tRNA            complement(319..393)
                     /locus_tag="JQR05_004296"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Gly,seq:tcc)
BASE COUNT          135 a           86 c           90 g           90 t
ORIGIN      
        1 gttgaaattt tgtccatgga tttcatagcc tcacctccgc aaataacgga tggcgtagtt
       61 ttacactgag aaatgaaagg gatttgaaaa gaaaaaaacg caaaagcggg cgaaacgata
      121 tatacagtaa ggaaagcact ctatccaaca aaccacccac agttaatcgg aataaaagca
      181 gagtgcttat gaatgatcgc acgcccgaag gttagtattt ctgcacagca attttgcaaa
      241 aaaaggagat cattcataac ttaaacgtct ttcagtcact ccgggatttc ccatcatcgc
      301 agactgaaag actctgactg gagcgggcag cgggaatcga acccgcatca tcagcttgga
      361 aggctgaggt aatagccatt atacgatgcc cgcatatggt g
//
LOCUS       DADKXM010000392          393 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.395, whole genome shotgun sequence.
ACCESSION   DADKXM010000392 DADKXM010000000
VERSION     DADKXM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.395"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            1..280
                     /locus_tag="JQR05_004297"
                     /pseudo
     CDS             1..280
                     /locus_tag="JQR05_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          101 a           90 c          104 g           98 t
ORIGIN      
        1 tagcctgaac gtgtactgtc gtaatggcta ggtggaaatc gacaacaaca tcggtgaaaa
       61 cgcgctgcga tcggtggcgg ttggaagaaa aaattatctc ttcttcggtt ctgacaacgg
      121 tggagaaagc gcggcgatta tctacagtct gctggtcacc tgcaaactga acggagtgga
      181 gccggaggac tggttgcgcg aagtgatcgt gaagctcaat gactggccgt cgaaccgagt
      241 gcatgaactg ctgccctgga acttctcagc tgtaaaataa tctttccgct acgtacttct
      301 ctgggcgctt accactcaac ggcatcgtca aaattatcaa agcgcagaac atagatttat
      361 catgtgtttt atggatggct ttctccatca ggc
//
LOCUS       DADKXM010000393          375 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.396, whole genome shotgun sequence.
ACCESSION   DADKXM010000393 DADKXM010000000
VERSION     DADKXM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.396"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>375
                     /locus_tag="JQR05_004298"
     CDS             <1..>375
                     /locus_tag="JQR05_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5326203.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADKXM010000394          360 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.397, whole genome shotgun sequence.
ACCESSION   DADKXM010000394 DADKXM010000000
VERSION     DADKXM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.397"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            22..291
                     /locus_tag="JQR05_004299"
                     /pseudo
     CDS             22..291
                     /locus_tag="JQR05_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           96 a           73 c           94 g           97 t
ORIGIN      
        1 ggcgcagcac gccacgccaa aatgctcgaa accgatcagg cgtgggatgt gttcgaaaaa
       61 ctggaagact gctatttcag tcaaaaggat ccgtcaacgc cagtttcatg ccagaaaagt
      121 tacgacacgt gagttctctg ttatcagaga ggcggtgtca ctgtttccac aattcagtta
      181 cgggatgatg atattgttat ttcccttgag tcatggctgg aactggcgag agccaatggt
      241 tggtttgttg ttcgcagaga taaactggtg gaaaggttga tgcagcttta aaaaagttct
      301 tgcatttttg cacataaact gcttcaattc cggtaagctt cgcaaagctg tatcgcgagg
//
LOCUS       DADKXM010000395          357 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.398, whole genome shotgun sequence.
ACCESSION   DADKXM010000395 DADKXM010000000
VERSION     DADKXM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.398"
                     /strain="IB1896"
                     /db_xref="taxon:623"
BASE COUNT          140 a           56 c           72 g           89 t
ORIGIN      
        1 gggcgcttac gtaataagta atctgacatc ccccgtgccc agaagaggag agggatatca
       61 agcccagggg ttaataattg atactacggt taaaattgcc ttcagtaaag gaaacgtgat
      121 taaattcagg ggatttcaga gtgaaaacca tacaacatat taaaatctgt tcatttagct
      181 ataatacata caagaaagtg tccagttaga taatgttatc taaataacca cagataaaaa
      241 cgcacataat tggggggggg atgaaaatac ctgaagcagt aaatcatatt aatgttcaga
      301 ataatattga tcttgttgat ggaaaaacga atccgaataa agcaacaaaa gcattac
//
LOCUS       DADKXM010000396          348 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.399, whole genome shotgun sequence.
ACCESSION   DADKXM010000396 DADKXM010000000
VERSION     DADKXM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.399"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(34..306)
                     /locus_tag="JQR05_004300"
     CDS             complement(34..306)
                     /locus_tag="JQR05_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326204.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMHPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           78 a           77 c           96 g           97 t
ORIGIN      
        1 gtgaacaggc gtttcagcgg tcggggctga aaatcaccac cacttcgggt catccgcctt
       61 catctccgct tctgtttcgt ataaatcaaa acgacggcag gtatggcaga acgtgacgta
      121 ttcgtacggt gaatccgggt gcattttgtg gaaggtttcc cagaaacaga ccgtgcagga
      181 gggatgtccg gtgataaagt ctgcaaatac ggacttataa gggtgattat tggctctggc
      241 gacggctttc agaacctctt tcaccattct ggtgtggact ttctggtgct ccaggttgtg
      301 tgacatggga actcattctg gatggttact ctgaaagctc atattctg
//
LOCUS       DADKXM010000397          346 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.400, whole genome shotgun sequence.
ACCESSION   DADKXM010000397 DADKXM010000000
VERSION     DADKXM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.400"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>346)
                     /locus_tag="JQR05_004301"
     CDS             complement(<1..>346)
                     /locus_tag="JQR05_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5326205.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIR"
BASE COUNT           98 a           89 c           64 g           95 t
ORIGIN      
        1 cacgtatcag ttttttttct ttaatagccc gctcaacctg atcataaggt agatacgcca
       61 atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt aaaagctgac
      121 cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga tttgctgtac
      181 ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac actgacgttg
      241 gagcacttcg acgagaaaaa taatccggtg agccaacaat agccattggt atatctggcc
      301 cgattcgaat ggcgatcata tctttatcca tctccccacc cagacg
//
LOCUS       DADKXM010000398          344 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.401, whole genome shotgun sequence.
ACCESSION   DADKXM010000398 DADKXM010000000
VERSION     DADKXM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.401"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>344
                     /locus_tag="JQR05_004302"
     CDS             <1..>344
                     /locus_tag="JQR05_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5326206.1"
                     /translation="AQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAA
                     AGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGRAFLPEDVIRFDTK
                     SDFLAWVRNALP"
BASE COUNT           87 a           89 c          106 g           62 t
ORIGIN      
        1 cgcacagcag acggcgcagg atgtggcggc agccgcaggt tatgcccgcg cagcagagca
       61 ggccaaaaat gacattgatg ctgcgctgac cggcactctg aaaacggcta accatctgtc
      121 tgaaatcgca gcagcaggcg aaaaggcaca acagaagtcc cgggataatc tggggctgaa
      181 aagtgcggcc acgatggaag cacagagcga catttacgac cggacaaaag gccgtctggc
      241 gatacccggc gcattcggct ttgggcgtgc ttttctgcct gaagatgtta tccgttttga
      301 cactaagagt gatttcctgg cctgggtaag gaatgcgctg ccag
//
LOCUS       DADKXM010000399          344 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.402, whole genome shotgun sequence.
ACCESSION   DADKXM010000399 DADKXM010000000
VERSION     DADKXM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.402"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>344
                     /locus_tag="JQR05_004303"
     CDS             <1..>344
                     /locus_tag="JQR05_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5326207.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DADKXM010000400          328 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.403, whole genome shotgun sequence.
ACCESSION   DADKXM010000400 DADKXM010000000
VERSION     DADKXM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.403"
                     /strain="IB1896"
                     /db_xref="taxon:623"
BASE COUNT           70 a           75 c           89 g           94 t
ORIGIN      
        1 gtaacaggat gagtttttct ttcgataata tttccctgaa taacttttcg ctcagtgaaa
       61 gtaacgtact gagtgcagtt cgtggcggcg gtgtcctggg actcattaac agtgtactgg
      121 caccgtcatt cggtatttat tacgcacgga atgatccggc aggtgcccat cagaagggtg
      181 gcaaaccttt ctccccggat tcttttgttg tcgttgaggt gggcgcggag gcttctgttt
      241 ccaccgtccc cgtggagcag ggggcttata ccacctttaa taaaatccag agatcgccgg
      301 aactgcatgt gactttcact gtagaggg
//
LOCUS       DADKXM010000401          320 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.404, whole genome shotgun sequence.
ACCESSION   DADKXM010000401 DADKXM010000000
VERSION     DADKXM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.404"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..301)
                     /locus_tag="JQR05_004304"
     CDS             complement(<1..301)
                     /locus_tag="JQR05_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326208.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNI"
BASE COUNT           88 a           87 c           60 g           85 t
ORIGIN      
        1 ggatattccc gttcagctgg acgatatccg cgacccgtac ctgcggacac agataccacc
       61 gcttcatatc cacaattctg aacagtcctt tcataaaact gacttccctt tctgtcagca
      121 gcgtcttctg acgttgccag tattcaccgc tggacagtat tcgctctgca tcaccggtcc
      181 gtatacctgc cgcatgcaat tgcatcagca aaggagaatg ctgatttaca cttctgagga
      241 taatccggat aagtagcgct gcaataatca gaacaatgag aataaaaata atcaacttca
      301 ttatttatct gctcctggca
//
LOCUS       DADKXM010000402          312 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.405, whole genome shotgun sequence.
ACCESSION   DADKXM010000402 DADKXM010000000
VERSION     DADKXM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.405"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>312)
                     /locus_tag="JQR05_004305"
     CDS             complement(<1..>312)
                     /locus_tag="JQR05_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5326209.1"
                     /translation="LRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLS
                     PAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGY
                     RL"
BASE COUNT           71 a          106 c           55 g           80 t
ORIGIN      
        1 agacggtagc cccgcttcag ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc
       61 agcgggtcaa ccttcagtaa cctctgatac atccctctcc aggtgatttg catcgccgtt
      121 tttctcactg tctccgttat gatgtacacc acttcttcca gtaaccgccg tttcgccgga
      181 ctcaaaaaac cgtagtacct caccatacgg aaccccttat ccgccacatg ccaggagaac
      241 ctttccatga actcatctcc actcatcaac aggtattctt cccgttttgt tcggtgactg
      301 ttgtaacgca ga
//
LOCUS       DADKXM010000403          307 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.406, whole genome shotgun sequence.
ACCESSION   DADKXM010000403 DADKXM010000000
VERSION     DADKXM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.406"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>307
                     /locus_tag="JQR05_004306"
     CDS             <1..>307
                     /locus_tag="JQR05_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY5326210.1"
                     /translation="WVEDEQGNKIQSPLMALPPLQRMEPGSKSLVKIQLAPGVEAKLP
                     QDRESLFYFNLREIPPKSNKANVLQLALQTRVKMFYRPKALAKNQAMNETPWQEQLTL
                     "
BASE COUNT           98 a           66 c           78 g           65 t
ORIGIN      
        1 ttgggtagag gatgagcaag gaaacaaaat acagtcaccg ctgatggcat tgccgccgct
       61 acaacgtatg gagccgggtt caaaaagcct ggtgaaaata cagttagcac caggcgttga
      121 agcaaaattg cctcaggatc gtgaatcgtt gttctatttt aatttacgcg aaattccacc
      181 taaaagtaat aaagcgaacg tcctgcaact ggcgctacag actcgggtga aaatgtttta
      241 tcgcccgaaa gcgctggcaa aaaaccaggc aatgaatgaa acaccgtggc aggagcagtt
      301 gacgctt
//
LOCUS       DADKXM010000404          306 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.407, whole genome shotgun sequence.
ACCESSION   DADKXM010000404 DADKXM010000000
VERSION     DADKXM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.407"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>306
                     /locus_tag="JQR05_004307"
     CDS             <1..>306
                     /locus_tag="JQR05_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY5326211.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
                     "
BASE COUNT           85 a           73 c           90 g           58 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
      301 tcgacg
//
LOCUS       DADKXM010000405          304 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.408, whole genome shotgun sequence.
ACCESSION   DADKXM010000405 DADKXM010000000
VERSION     DADKXM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.408"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..303
                     /locus_tag="JQR05_004308"
     CDS             <1..303
                     /locus_tag="JQR05_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY5326212.1"
                     /translation="KRLKATYRRAKTITLIVDNYIIHKSRETQSWLKGNPKFRVIYQP
                     VYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT           91 a           76 c           77 g           60 t
ORIGIN      
        1 aagcggctta aagcgacata ccgtcgggcg aaaaccatca cgctgatcgt ggacaactac
       61 attatccaca aaagccggga aacacagagc tggctgaagg ggaacccgaa gttcagggtc
      121 atttatcagc cggtttactc gccatgggtg aatcatgttg aacggctatg gcaggcactt
      181 cacgacacaa taacgcgtaa tcatcagtgc cgctcaatgt ggcaactgtt gaaaaaagtt
      241 cgccatttta tggaaaccgt cagcccattc cccggaggca aacatggact ggcaaaagtg
      301 tagc
//
LOCUS       DADKXM010000406          304 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.409, whole genome shotgun sequence.
ACCESSION   DADKXM010000406 DADKXM010000000
VERSION     DADKXM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.409"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..259)
                     /locus_tag="JQR05_004309"
     CDS             complement(<1..259)
                     /locus_tag="JQR05_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY5326213.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML"
BASE COUNT           88 a           72 c           83 g           61 t
ORIGIN      
        1 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
       61 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
      121 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
      181 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
      241 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
      301 actg
//
LOCUS       DADKXM010000407          302 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.410, whole genome shotgun sequence.
ACCESSION   DADKXM010000407 DADKXM010000000
VERSION     DADKXM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.410"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>302
                     /gene="virK"
                     /locus_tag="JQR05_004310"
     CDS             <1..>302
                     /gene="virK"
                     /locus_tag="JQR05_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY5326214.1"
                     /translation="GAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECL
                     AVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIA"
BASE COUNT           79 a           61 c           78 g           84 t
ORIGIN      
        1 ggagccccta aaaataccgg acctgatgtt attcggtgcg caacccgggc ctgttatggg
       61 attttcccaa aacggattat ttttgaagca ttttgtgcgt tgatgaaagc ttgcaatatc
      121 tctgagtgtc tggcagtcag tgagcacagt catgttttcc ggcaattgag atactggtat
      181 cagaaacgca aaacctttgt tgcggtctat agtgatttct gggagtctgt agcaggaaaa
      241 acctgtggtg actggtacag attaccaacc caggtgattc gaaagccgct aagcgatata
      301 gc
//
LOCUS       DADKXM010000408          295 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.411, whole genome shotgun sequence.
ACCESSION   DADKXM010000408 DADKXM010000000
VERSION     DADKXM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.411"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..>295)
                     /locus_tag="JQR05_004311"
     CDS             complement(<1..>295)
                     /locus_tag="JQR05_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5326215.1"
                     /translation="RDVIVCAEMDEQWSYVGAKSRQRWLFYAYDRVRRTVVAHVFGER
                     TMFTLERLLSLLSAFDVVVLMTDGWPLYESRLKEKLHVISKRYTQRIERHNLR"
BASE COUNT           66 a           94 c           70 g           65 t
ORIGIN      
        1 tgcctcagat tatgccgctc aattcgctgc gtatatcgct tgctgataac gtgcagcttt
       61 tccttcaggc gggattcata aagcggccat ccgtccgtca tcaataccac cacgtcaaag
      121 gccgacagca ggctcagaag acgctccagc gtgaacattg tgcgttcacc aaaaacgtgg
      181 gccacgacag tcctgcgtac cctgtcatac gcgtaaaaca gccagcgctg gcgtgattta
      241 gccccgacgt agctccactg ttcgtccatt tccgcgcaga cgatgacgtc cctgc
//
LOCUS       DADKXM010000409          293 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.412, whole genome shotgun sequence.
ACCESSION   DADKXM010000409 DADKXM010000000
VERSION     DADKXM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.412"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(22..98)
                     /locus_tag="JQR05_004312"
     tRNA            complement(22..98)
                     /locus_tag="JQR05_004312"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Arg,seq:tct)
     gene            complement(100..176)
                     /locus_tag="JQR05_004313"
     tRNA            complement(100..176)
                     /locus_tag="JQR05_004313"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Ile,seq:cat)
BASE COUNT           71 a           84 c           73 g           65 t
ORIGIN      
        1 cctgctgagc taagtcggcg ctggcccacc accgaggact cgaacctcgc accgtcaact
       61 tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg
      121 aaccagcgac ctggcgatta tgagtcgctc gctctgacca actgagctaa agggccggag
      181 gcagaataat aaccatatgt catcacatct gcaaactcat ctgaccacca gcgcgtttaa
      241 cgccctgtgc catttttcag gcacaaaaaa acccgcataa agcgggttct ttc
//
LOCUS       DADKXM010000410          263 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.413, whole genome shotgun sequence.
ACCESSION   DADKXM010000410 DADKXM010000000
VERSION     DADKXM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.413"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            <1..>263
                     /locus_tag="JQR05_004314"
     CDS             <1..>263
                     /locus_tag="JQR05_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326216.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREM"
BASE COUNT           53 a           79 c           68 g           63 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gtt
//
LOCUS       DADKXM010000411          263 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.denovo.414, whole genome shotgun sequence.
ACCESSION   DADKXM010000411 DADKXM010000000
VERSION     DADKXM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.denovo.414"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..230)
                     /locus_tag="JQR05_004315"
     CDS             complement(<1..230)
                     /locus_tag="JQR05_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5326217.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGDGDGGLTTAERQRLKEL"
BASE COUNT           56 a           78 c           61 g           68 t
ORIGIN      
        1 tccagctctt tcagacgctg acgttcagcg gtggtgagcc ctccatcacc gtccccggta
       61 tcccgctcat gctggcgaac ccagacacgc agagtctccg gcgtacagcc aatctttgga
      121 gcaatggaac aaattgtcgc ccattgtgag tcatattcgc cctgactttc cagaaccata
      181 cggactgccc gttgacggac ttcgggggaa aaacgagtat ttttagtcat cctgtttacc
      241 tctttctcag gaagtttagt ctc
//
LOCUS       DADKXM010000412        18573 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.guided.073, whole genome shotgun sequence.
ACCESSION   DADKXM010000412 DADKXM010000000
VERSION     DADKXM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.guided.073"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JQR05_004316"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JQR05_004316"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY5326218.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JQR05_004317"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JQR05_004317"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY5326219.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3002
                     /gene="psd"
                     /locus_tag="JQR05_004318"
     CDS             2040..3002
                     /gene="psd"
                     /locus_tag="JQR05_004318"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY5326220.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKD"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JQR05_004319"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JQR05_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY5326221.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMSQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JQR05_004320"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JQR05_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY5326222.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDMNFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JQR05_004321"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JQR05_004321"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY5326223.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JQR05_004322"
                     /pseudo
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JQR05_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
     gene            11316..12050
                     /locus_tag="JQR05_004323"
     CDS             11316..12050
                     /locus_tag="JQR05_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY5326224.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JQR05_004324"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JQR05_004324"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY5326225.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JQR05_004325"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JQR05_004325"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY5326226.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /gene="blaEC"
                     /locus_tag="JQR05_004326"
     CDS             12889..14022
                     /gene="blaEC"
                     /locus_tag="JQR05_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY5326227.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            14111..14644
                     /locus_tag="JQR05_004327"
     CDS             14111..14644
                     /locus_tag="JQR05_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY5326228.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14958)
                     /locus_tag="JQR05_004328"
     CDS             complement(14641..14958)
                     /locus_tag="JQR05_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY5326229.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15134..15280)
                     /gene="ecnB"
                     /locus_tag="JQR05_004329"
     CDS             complement(15134..15280)
                     /gene="ecnB"
                     /locus_tag="JQR05_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY5326230.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15391..15516)
                     /gene="ecnA"
                     /locus_tag="JQR05_004330"
     CDS             complement(15391..15516)
                     /gene="ecnA"
                     /locus_tag="JQR05_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY5326231.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15568..16134)
                     /gene="efp"
                     /locus_tag="JQR05_004331"
     CDS             complement(15568..16134)
                     /gene="efp"
                     /locus_tag="JQR05_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY5326232.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16176..17204
                     /gene="epmB"
                     /locus_tag="JQR05_004332"
     CDS             16176..17204
                     /gene="epmB"
                     /locus_tag="JQR05_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY5326233.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17599..18468
                     /locus_tag="JQR05_004333"
     CDS             17599..18468
                     /locus_tag="JQR05_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5326234.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4690 a         4829 c         4736 g         4318 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgtatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcatgca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagact
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgt cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gatgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg ctgtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg ctttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgagca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcaact tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acagcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattcatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga tacctggatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct agttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcaat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 agaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc cttctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactagccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca tcgcccaggc
    14821 aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg tcaaacggct
    14881 aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac
    14941 taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg tgaaaagagg
    15001 cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg
    15061 aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga cactgctgtc
    15121 gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat
    15181 cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca
    15241 ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa
    15301 tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgatggaa
    15361 atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga tggagttgcc
    15421 gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga gcagcgtgct
    15481 ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg
    15541 cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg
    15601 ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa ccacagcgcc
    15661 agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc
    15721 ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt
    15781 ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcgttgt cgccaattgc
    15841 ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc
    15901 gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt
    15961 agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc
    16021 aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc
    16081 gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc
    16141 ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata
    16201 ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac
    16261 ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa
    16321 agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg
    16381 acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgtccggat
    16441 tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc
    16501 acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc
    16561 gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgcacttg
    16621 agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga
    16681 tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca
    16741 aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc accgaggcgc
    16801 tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac atcaatcatg
    16861 ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt gtcggtgtca
    16921 ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa acgttggcaa
    16981 acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat gtgctagata
    17041 aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg
    17101 agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa attggcggcg
    17161 aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa
    17221 gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt gcattgaaat
    17281 tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat ccggcaagag
    17341 caacaaacgc aagtacagca acctgtcatt aaactgagaa atttaatctt tgcacgttgc
    17401 ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct aacaccaata
    17461 attaaccttt cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac
    17521 tattactacc aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa
    17581 tggttaggga gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc
    17641 aagagtaata atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg
    17701 ttgttcttta tgtctgtcat ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg
    17761 catcaaaagc ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa
    17821 gtcattaaga aaatggcaga aaacatccca gaaattctgg ttgatgagct taaaaatgcc
    17881 gatattaatc gtcgggttaa cacgctggaa cttaccgata atcaaggcga aaatcttacc
    17941 ttcgtattaa cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt
    18001 gaaatgctgg cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca
    18061 ttacgtatta cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggacaat
    18121 ggcaatctgg aatatgacac ctattcccaa ccggaatgga aacataactt gttcgaccat
    18181 tatctggcgg tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca
    18241 gtggtgaaaa cgcgtgaagc aacgccgggt aaagaaatag aggcgatcac tcgccgaatg
    18301 cttgatttca gcccacgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc
    18361 accgtagcgg cgaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat
    18421 cacttacgtg tacaatccac cagcaaaact gcaccacagg cgaaataatc tcatctttgc
    18481 taatgcgagg ctggagccat ccagcctgaa atgactgata acgtgatcgt tggtcatgcc
    18541 ggatgcggcg taaacgcctt atccggccta caa
//
LOCUS       DADKXM010000413         9981 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.guided.141, whole genome shotgun sequence.
ACCESSION   DADKXM010000413 DADKXM010000000
VERSION     DADKXM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.guided.141"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            61..585
                     /locus_tag="JQR05_004334"
                     /pseudo
     CDS             61..585
                     /locus_tag="JQR05_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            701..1261
                     /locus_tag="JQR05_004335"
                     /pseudo
     CDS             701..1261
                     /locus_tag="JQR05_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1318..2775)
                     /gene="pepD"
                     /locus_tag="JQR05_004336"
     CDS             complement(1318..2775)
                     /gene="pepD"
                     /locus_tag="JQR05_004336"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY5326235.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3037..3495
                     /gene="gpt"
                     /locus_tag="JQR05_004337"
     CDS             3037..3495
                     /gene="gpt"
                     /locus_tag="JQR05_004337"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY5326236.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3587..4831
                     /gene="frsA"
                     /locus_tag="JQR05_004338"
     CDS             3587..4831
                     /gene="frsA"
                     /locus_tag="JQR05_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY5326237.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4889..5290
                     /locus_tag="JQR05_004339"
     CDS             4889..5290
                     /locus_tag="JQR05_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY5326238.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JQR05_004340"
     CDS             complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JQR05_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY5326239.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6672..7775
                     /gene="proB"
                     /locus_tag="JQR05_004341"
     CDS             6672..7775
                     /gene="proB"
                     /locus_tag="JQR05_004341"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY5326240.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7787..9040
                     /gene="proA"
                     /locus_tag="JQR05_004342"
     CDS             7787..9040
                     /gene="proA"
                     /locus_tag="JQR05_004342"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY5326241.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDKSAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADSAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9155..9230
                     /locus_tag="JQR05_004343"
     tRNA            9155..9230
                     /locus_tag="JQR05_004343"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9188..9190,aa:Thr,seq:cgt)
     gene            complement(9245..9943)
                     /locus_tag="JQR05_004344"
                     /pseudo
     CDS             complement(9245..9943)
                     /locus_tag="JQR05_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2391 a         2483 c         2609 g         2498 t
ORIGIN      
        1 atcagatcgc acaatccggg ctaagttccc tcaaagtgat ttactattcc gcgcagctat
       61 ttagggcagt ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa
      121 ggcagtgacg acgcgctggc gtttgcgcgt tttaatcgcc acctgatcgc tttgcgcata
      181 atgcaacagg ttaaggccac cggaagtccg gttctggatg tggcgcataa ctttgttagc
      241 gcgtgtcgaa tcggtgatca acaaggggtc ttacatcgta aaggtgccac gcctgatgac
      301 tgtggtctgg tggttattcc cggttcacgc ggtgattact cctggctggt tcagcccgtc
      361 aggagtgagg aaacattgca ttcgctggcg catggggctg ggcgtaaatg ggggcgcacc
      421 gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc aactctcgcg tactgaactt
      481 ggcagtcggg taatttgtcg cgataaacaa ctcatcttcg aagaagcgcc acaagcctat
      541 aaatcggctg aaagtgtggt gcaatgtctg ggcaggccgg attaattatt cctgtcgcgc
      601 ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctata
      661 actctcctca gtgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag
      721 gctaattaaa gaagctgccc gacaagacgt cgcggtaacg gtgctggaaa cagaaacagg
      781 ccgctactct gacacgctgc gttcggcgct gatttctctg gatggcgaca acgcatgggc
      841 gttaagcgaa agctggtgtg gcactattca gtggatttgc ccgagcccgt atcgcccaca
      901 tcacgggcgt aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggagca
      961 atccgatgcg atccggtatg agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa
     1021 taaaaccgac tcggcggtac gcgccacgca tttggcatcc ggtattagcg tgaaggttca
     1081 gtcagagcgc agccagcatg ctaacaaacg gctggcgcga ttgctgattg cctggaagct
     1141 ggatcagcag caataggaaa atagcgcggt gctgaaatca cagcggcgaa tgttccatca
     1201 ccagattgaa cgtggcaacc cgcgacgaac gtttacaggg atggctttta tcgaaggata
     1261 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
     1321 cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc
     1381 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
     1441 gtccatttcc ggatacggtt ttttgaacag accacattct aggcccgcgt ggataatctg
     1501 gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtaccagat gcatcaccgg
     1561 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
     1621 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagtga
     1681 acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
     1741 ggaggtttcg accacacctt ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc
     1801 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
     1861 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
     1921 ctcctgatag gtattcacca gagatttcag tgcgtcgact ttatcagctg cgacagcaat
     1981 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
     2041 gcgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt
     2101 acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa
     2161 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     2221 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     2281 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
     2341 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
     2401 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
     2461 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     2521 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc
     2581 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     2641 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     2701 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     2761 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     2821 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt attgagaata
     2881 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     2941 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     3001 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     3061 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     3121 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt
     3181 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     3241 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     3301 gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac
     3361 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     3421 atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     3481 atctccggtc gctaatcttt tcaacgcctg gcattgccgg gcgttgttct ttttaacttc
     3541 aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     3601 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     3661 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
     3721 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
     3781 aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     3841 atacgatacc gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     3901 gatggtgtgg caacaaaaag cctgtacgga agaagatccg caactcagtg gtcgacactg
     3961 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     4021 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     4081 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     4141 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     4201 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat
     4261 gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     4321 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     4381 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct
     4441 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     4501 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     4561 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     4621 agtgcaagga ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     4681 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     4741 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     4801 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa
     4861 aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag
     4921 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     4981 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     5041 tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta cctacagcta
     5101 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagatactga
     5161 agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct
     5221 gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgatgaac cggtgaagtt
     5281 aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatattcatt aaaactgata
     5341 cgtcatccca accgcgacag tatcatcatt attaatatta agcttattat cgctatccag
     5401 ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat acgtagcacc
     5461 gacgtcgata taattgacca gatcttcatc accgatgccc tcaatatctt tccctttcga
     5521 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     5581 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga
     5641 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     5701 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     5761 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     5821 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     5881 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     5941 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa
     6001 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
     6061 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcagtatc
     6121 actctccgct ttatttccgg caaactccgc ttcccaccga ccataaccag tcagttgatc
     6181 gttaatttga gtttcacctt taaaaccaaa acggatataa ctctggtcgc catctttact
     6241 ggcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc
     6301 gtctttatta tatatttctg cagcctgtac agatgcagat gccacaatgc ccatcaccac
     6361 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     6421 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     6481 cagattaatg atattctgaa aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca
     6541 actgccgcta cgcttgctga tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt
     6601 ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct aaaattgaat
     6661 ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc agtgtgctaa
     6721 caggcggatc gcgccgtctg aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc
     6781 agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc gccgccggac
     6841 gtgagcacct gggttacccg gaactgccag cgactatcgc ctcgaaacaa ctgctggcgg
     6901 cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt tatggcattc
     6961 acgtcgggca aatgctgctg acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg
     7021 cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc aatgagaacg
     7081 atgctgtcgc tacggcagag attaaggtcg gcgataacga taacctttct gcgctggcgg
     7141 cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg
     7201 ctgatccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc attgatgacg
     7261 cactgcgcgc gattgccggt gacagcgttt caggcctcgg aactggcggc atgagtacca
     7321 aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga caccattatt gccgcgggca
     7381 gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg
     7441 cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg
     7501 aaatcacggt agatgaaggg gcaactgccg ccattctgga acgcggcagc tccctgttgc
     7561 cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca
     7621 acctcgaagg tcgcgatatc gcccacggtg tcagtcgtta caacagcgat gcattacgcc
     7681 gtattgccgg acaccactcg caagaaattg atgcaatact gggatatgaa tacggcccgg
     7741 ttgccgttca ccgtgatgac atgattaccc gttaaggagc tggccgatgc tggaacaaat
     7801 gggcattgcc gcgaagcaag cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa
     7861 tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc
     7921 taacgcccag gatgttgctg acgcgcgagc caatggcctt agcgaagcga tgcttgaccg
     7981 tctggcactg acgcccgcac ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa
     8041 cctcgccgat ccggtggggc aggtaatcga tggcggcgta ctggacagcg gcctgcgtct
     8101 tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt
     8161 gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat gcggtgatcc tgcgtggtgg
     8221 taaagaaacc tgtcgcacta acgcggcaac ggtggcggtg attcaggacg ccctgaaatc
     8281 ctgtggctta ccggcgggtg ccgtgcaggc gattgataat cctgaccgtg cgctggtcag
     8341 tgaaatgctg cgtatggata aatacatcga catgctgatc ccgcgtggtg gcgctggttt
     8401 gcataaactg tgccgtgaac agtcgacaat cccggtgatc acaggtggta taggcgtatg
     8461 ccatatttac gttgataaaa gtgcagagat cgctgaagca ttaaaagtga tcgtcaatgc
     8521 gaaaactcag cgtccgagca catgtaatac ggttgaaacg ttgctggtga ataaaaacat
     8581 cgccgatagc ttcctgcccg cattaagcaa acaaatggcg gaaagcggcg tgacattaca
     8641 cgcagattca gctgcgctga cacagttgca ggcaggccct gcgaaggtgg tggcggttaa
     8701 agccgaagag tatgacgatg agtttctgtc attagatttg aacgtcaaaa tcgtcagcga
     8761 tcttgacgat gccatcgccc atattcgtga acacggcaca caacactccg atgcgatcct
     8821 gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa gtggattcgt ccgctgttta
     8881 cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt ggtctgggtg cggaagtggc
     8941 ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg ctggaagcac tgaccactta
     9001 caagtggatc ggcattggtg attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa
     9061 agtagccatt tgattcacaa ggccattgac gcatcgcccg gttagtttta accttgtcca
     9121 ccgtgattca cgttcgtgaa catgtccttt cagggccgat atagctcagt tggtagagca
     9181 gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca ttaaaatcaa
     9241 taagttacac atcattagta ccttccttat tttttgactg ggacaaattt gggaccgatg
     9301 ggttcaggat cgagtctatt tgccgtgcgt gttcggtaag gtgattaggt gcaaggtgag
     9361 catatcgacg aaccatttcg atagactccc agcctcccat ttcctgtaac actgacaacg
     9421 ggactccggc ttgaaccagc caacttgccc aggtgtgtct caagtcgtga aatctgaaat
     9481 catcaatacc agcccgtctc agcgccgctt tccaggctgt gtttgcgtca taccgcatct
     9541 tccttactgt tggcgctttc gttccgtctg gtttggtaca gctttccttg tacacaaata
     9601 cccaacggtg atgattcccg atttgttttt tcaaaacgcg acatgcagta tcattcagcg
     9661 caacgccgat tgcgcggttt gatttactct cttccgggtt tatccatgcc acccggcgct
     9721 gcatatctat ttgttgccat tcaaggttga tgatgttcga gcgtcttaag cctgttgcca
     9781 gtgcaaattc aacaacagac tttaatggct ccggacattc atcaatcagc ctttgtgctt
     9841 catggggctc cagccagcgg atccgtttat tctttggttg aggcacttta ataattggtg
     9901 ccttatccag cattttccat tcacgctctg cggctcttag tagtaatgcc agtcagttaa
     9961 gcaactgact ggctcttttt c
//
LOCUS       DADKXM010000414         8000 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain IB1896
            SAMEA1030433-rid11441703.guided.156, whole genome shotgun sequence.
ACCESSION   DADKXM010000414 DADKXM010000000
VERSION     DADKXM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030433
            Sequence Read Archive: ERR048311
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000945792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000945792.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/02/2021 03:02:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,352
            CDSs (total)                      :: 4,297
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 148 of 316
            Pseudo Genes (incomplete)         :: 83 of 316
            Pseudo Genes (internal stop)      :: 120 of 316
            Pseudo Genes (multiple problems)  :: 31 of 316
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030433-rid11441703.guided.156"
                     /strain="IB1896"
                     /db_xref="taxon:623"
     gene            complement(<1..112)
                     /locus_tag="JQR05_004345"
     CDS             complement(<1..112)
                     /locus_tag="JQR05_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5326242.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRK"
     gene            391..1050
                     /locus_tag="JQR05_004346"
     CDS             391..1050
                     /locus_tag="JQR05_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY5326243.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1251..1628)
                     /locus_tag="JQR05_004347"
     CDS             complement(1251..1628)
                     /locus_tag="JQR05_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5326244.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1695..4661)
                     /locus_tag="JQR05_004348"
     CDS             complement(1695..4661)
                     /locus_tag="JQR05_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY5326245.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4664..5224)
                     /locus_tag="JQR05_004349"
     CDS             complement(4664..5224)
                     /locus_tag="JQR05_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY5326246.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5350..5700)
                     /locus_tag="JQR05_004350"
     CDS             complement(5350..5700)
                     /locus_tag="JQR05_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY5326247.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5903..6916)
                     /gene="intI1"
                     /locus_tag="JQR05_004351"
     CDS             complement(5903..6916)
                     /gene="intI1"
                     /locus_tag="JQR05_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY5326248.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            7065..7856
                     /gene="aadA1"
                     /locus_tag="JQR05_004352"
     CDS             7065..7856
                     /gene="aadA1"
                     /locus_tag="JQR05_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY5326249.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1687 a         2209 c         2366 g         1738 t
ORIGIN      
        1 ttttacggca gtgagagcag agatagcgct gatgtccggc ggtgcttttg ccgttacgca
       61 ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc actggagcac
      121 ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc
      181 gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
      241 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
      301 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
      361 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      421 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      481 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      541 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      601 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      661 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      721 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      781 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      841 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      901 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
      961 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     1021 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
     1081 acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa
     1141 gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt
     1201 gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct
     1261 gaggccagtt tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt
     1321 ctgcctccca gagcctgata aaaacggtta gcgccggggt tggatttttc agcgttccag
     1381 ctaaggctaa ggcattcctg ttcaagcgca agccgggcta taaagcgcat tatcgcttta
     1441 cccgtgccct tatttcgatc gcactgagaa acatacagct ctttaatatg cagctgaccg
     1501 ctgtatcggg gcgagggata aagaatattg caacatgcca ggccagtaat gttattgccg
     1561 cagcgcgctc tgatcaccag ggtaccggaa agccggttga ataacttttt acagagataa
     1621 tccttcatca acgcttcctg aatgatacct tcgccataat aggggtcgtc tcagaaaacg
     1681 gaaaataaag cacgctaagc cggttgcaga ggccgtagcg gcctgaactt ccccgcgccg
     1741 atcttggcgc tgctgcgcca taggtaatca ccggtcaggt tgatgtgctc ccagccgagt
     1801 ggcgacaggt actgcaatag cgagtcatcg acggcatgac cattgccgcg caacgcatgc
     1861 gccgcacgct ccaggtagac cgtgttccac agcacgatgg ccgccgtcac caggttgagg
     1921 ccgctggccc ggtagcgctg ctgctcgaaa ctgcggtcac ggatttcacc aaggcggttg
     1981 aagaacacgg cacgggccag cgcattgcgc gcctcgccct tgttcagccc ggcatgcacg
     2041 cggcggcgta gctcgacgct ttgcagccag tcgaggatga acagcgtgcg ctcgatgcgg
     2101 cccaactcgc gcagcgcgac ggccaagccg ttctggcgcg ggtagctgcc gagtttcctg
     2161 agcatcagcg aggccgtcac cgtgccctgc ttgatcgagg tggccagccg caggatttcg
     2221 tcccaatggg cgcggacgtg cttgatgttg agcgtgccgc cgatcatcgg cttgagcgcg
     2281 tcataggcgg catcgccctt cgggatgtag agcttggtgt cgcccaggtc gcggatgcgc
     2341 ggcgcgaagc ggaagcccaa gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc
     2401 gtgtcggtgt agtgctcctc gatccgcagg tcggattcgt ggtacagcag gccgtcgagc
     2461 acgtaggttg agtcgcgcag gccgacattg accaccttgg tgtggaatgg cgcgtattgg
     2521 tcggagatgt gggtgtagaa agtccgtcct gggctgctgc catattttgg gttgatgtgc
     2581 cccgtgctct ttgccttgct agcggttcgg aaattctgtc cgtccgatga tgatgtggtg
     2641 ccatcgcccc agtgcccggc aaagggatgc cgaaactgag cgttgaccag ttcagccaac
     2701 gctgtcgagt acgtttcgtc gcgggtatgc caggcttgca gccaagcgag cttcgcgtag
     2761 gtcgtgccgg ggcaggactc ggccatcttg gtcaggccca ggttgatcgc gtcggccagg
     2821 atcgtggtca acaacaggtt cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag
     2881 tgccgggtga agcccgtcca ctcatcgact tcgagcagca gttcggtgat cttgacgtgc
     2941 ggcaggacca tggctgtctg gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc
     3001 agcggcgtga tcttcaagcc cgactcggtg atgatggcat ccggcaggtc gttggctgcc
     3061 gccatgcggt tgacggtggc aagttgtgct tccagcagcg tcagccgctc atgcagatat
     3121 tgttcgcagt cggtggccac ggccagcggc aattcgctgg actgcttgag gctggtgaac
     3181 ttctcgggcg gtaccaggta gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag
     3241 atgtcgcccg agcgcaggga gttcttcaac tcggacagcg cgcacagttc gtagtagcgc
     3301 cggtcgatgc cggcgtcggt catcaccagt ttctgccagc gcggcttgat gaagccggtc
     3361 ggtgcatcgg ctggcagctt gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg
     3421 gcatcaagca cgtttttggc ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc
     3481 ggtgcatagc ggcgcagggt ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca
     3541 tcgggttgcg cgagcttctg cgcctcggtg acgctctcgg caaaggaatc ccaggacatg
     3601 acggcctcga tggcggcaaa cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga
     3661 ccgatgcgcc cgtacagacg taccttggcg ttgatggcct tgcctgacgc ctggaactgc
     3721 tgctgatgct tattcttggc agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg
     3781 atgatttcgt cggtgacggt ggccatgccc tcggtggcca gcgccacgag agtggcgtag
     3841 cgccgttgcg gctcgaattt ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca
     3901 atcttgagca ggcggttctg gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc
     3961 tgccatgcct tgaggcgttc gatgtgttcc agcatatgcc gcgaatttgg cttggccgga
     4021 gactggcgca accaagccaa ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg
     4081 tcgaggcggc ggcgatgcgc gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg
     4141 ttagcacggg tgatcgcctc ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg
     4201 accgactgcc gccgcaggtg cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt
     4261 tgcatcgcca gctcggtcag catctggacg gcctgccggt aatggctcat ggtgaagggc
     4321 cggaaaccga acacggtttg cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg
     4381 ccgtactcgt tccagctttc gacgccgacc ttgagctggt cggcgaccag cttcaacaag
     4441 ggcgggaacg gtagttcatc gacgcccagg atgacgccgg gaaagcgcag gtaacagagc
     4501 tgcaccgcga agcccagccg attggctggc ccgcgccgct gtcggatgat cgagaggtcg
     4561 gtatcgttga atgtgtaatg tcggatcagg tcgtccttgg agtccggcaa cgccagcagg
     4621 ctttcccgct cggcggcgga caggatggaa cgacgtggca tatttactga tccgttctca
     4681 agtattgata cagggtttcg cgactgattc cgaattcacg agcaagcttg gtcttttgct
     4741 cgccagcctc gacacgttgg cgcagttcgg caatacgctc agacgacagg gatttcttcc
     4801 tgccacggta agccccgcgt tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga
     4861 tcagggcgcg ctcgaactcg gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg
     4921 gagagtcttc gccagtaaaa ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt
     4981 gtgtcagcgt ttgcacgatc cggcgcaaat catcgagatt gcgcgccagg cgatccatgc
     5041 tatgcaccac cacggtgtcg ccggtgcggg cgaagcttat cagcgcttcc agttgcggac
     5101 gcttgacatc cttgccggat gccttgtcgc taaaagcgcg atcaaccttg acgccttcca
     5161 gttgccgttc cgggttctgg tcgaaggtgc tgaccctgat atacccaatg cgctgtccag
     5221 tcatggaatt ccctgcaaaa tgtcagggaa gactctatga ccttcaacga gatatgtcaa
     5281 taaattcaaa attcaatcct atcctgacgc aatttacaca tggcatctga catcaggtta
     5341 gggtatgcct caacctgacg gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt
     5401 cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc agcccgcaga aatgctccac
     5461 gtactcggcc ccttccggcg tgaaggcggc atcgagcggg atttccttgc agcacacgca
     5521 gcaactggtg ctcggttcat tggcgttcat ggtggtgttc ctccattggt tgacgaagcc
     5581 gacgaaggcc gccgccggca tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc
     5641 cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca ccctcggcta ccacctccat
     5701 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     5761 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
     5821 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
     5881 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
     5941 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
     6001 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
     6061 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
     6121 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
     6181 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
     6241 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
     6301 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
     6361 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
     6421 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
     6481 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
     6541 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
     6601 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
     6661 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     6721 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     6781 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     6841 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     6901 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     6961 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     7021 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     7081 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     7141 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     7201 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     7261 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     7321 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     7381 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     7441 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     7501 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     7561 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     7621 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     7681 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     7741 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     7801 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     7861 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     7921 ctaagcacat aattgctcac agccaaacta tcaggtcaag tcggtgatgc taccaacttg
     7981 ctgatttagt gtataatggt
//
